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Sample records for 1-antichymotrypsin antithrombin iii

  1. Antithrombin III blood test

    MedlinePlus

    ... AT III) is a protein that helps control blood clotting. A blood test can determine the amount of ... may mean you have an increased risk of blood clotting. This can occur when there is not enough ...

  2. Antithrombin III blood test

    MedlinePlus

    ... be due to: Bone marrow transplant Disseminated intravascular coagulation (DIC) AT III deficiency, an inherited condition Liver ... Schmaier AH, Miller JL. Coagulation and fibrinolysis. In: McPherson ... Management by Laboratory Methods . 22nd ed. Philadelphia, PA: ...

  3. [Role of antithrombin iii in cardiac surgery].

    PubMed

    Muedra, V; Barettino, D; D'Ocón, P

    2013-11-01

    Coagulation of blood is of multidisciplinary interest. Cardiac surgery produces major changes in the delicate balance between pro-and anti-coagulant serum factors. The role of antithrombin iii has been analysed after finding evidence that associated decreased levels of protein activity to postoperative morbidity and mortality. Supplementing exogenous antithrombin is considered with the aim of optimising outcomes. Its intrinsic anticoagulant and anti-inflammatory properties have stimulated a growing interest, and suggests new lines of research. PMID:23228672

  4. 21 CFR 864.7060 - Antithrombin III assay.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Antithrombin III assay. 864.7060 Section 864.7060 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7060 Antithrombin...

  5. 21 CFR 864.7060 - Antithrombin III assay.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Antithrombin III assay. 864.7060 Section 864.7060 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7060 Antithrombin...

  6. 21 CFR 864.7060 - Antithrombin III assay.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Antithrombin III assay. 864.7060 Section 864.7060 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7060 Antithrombin...

  7. 21 CFR 864.7060 - Antithrombin III assay.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Antithrombin III assay. 864.7060 Section 864.7060 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7060 Antithrombin...

  8. 21 CFR 864.7060 - Antithrombin III assay.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Antithrombin III assay. 864.7060 Section 864.7060 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7060 Antithrombin...

  9. Molecular evolution of serpins: homologous structure of the human. cap alpha. /sub 1/-antichymotrypsin and. cap alpha. /sub 1/-antitrypsin genes

    SciTech Connect

    Bao, J.; Sifers, R.N.; Kidd, V.J.; Ledley, F.D.; Woo, S.L.C.

    1987-12-01

    ..cap alpha../sub 1/-Antichymotrypsin belongs to a supergene family that includes ..cap alpha../sub 1/-antitrypsin, antithrombin III, ovalbumin, and angiotensinogen. The human chromosomal ..cap alpha../sub 1/-antichymotrypsin gene has been cloned and its molecular structure established. The gene is approximately 12 kb in length and contains five exons and four introns. The locations of the introns within the ..cap alpha../sub 1/-antichymotrypsin gene are identical with those of the human ..cap alpha../sub 1/-antitrypsin and angiotensinogen genes. Other members of this supergene family contain introns located at nonhomologous positions of the genes. The homologous organization of the ..cap alpha../sub 1/-antichymotrypsin and ..cap alpha../sub 1/-antitrypsin genes corresponds with the high degree of homology between their protein sequences and suggest that these loci arose by recent gene duplication. A model is presented for the evolution of both the genomic structure and the protein sequences of the serine protease inhibitor superfamily.

  10. Acquired antithrombin III deficiency: laboratory diagnosis, incidence, clinical implications, and treatment with antithrombin III concentrate.

    PubMed

    Büller, H R; ten Cate, J W

    1989-09-11

    Antithrombin III (ATIII) is the predominant naturally occurring inhibitor of serine proteases generated during blood coagulation [Rosenberg RD: Annu Rev Med 1978; 29: 367-378]. Since 1965, several assays have been developed that allow rapid and precise determination of ATIII in plasma. As a consequence, the existence of acquired ATIII deficiency in many pathologic conditions has been described. Acquired ATIII deficiency is based on decreased synthesis, increased loss or increased consumption, or induced by drugs. An inherited ATIII deficiency is associated with a lifelong tendency to venous thromboembolism. In contrast, the clinical significance of acquired ATIII deficiency has been less well defined. A precise estimate of the risk of thromboembolism in the acquired ATIII deficiency state cannot easily be provided, owing to the lack of studies in consecutive patients. In 1978, a purified human ATIII concentrate became available for clinical investigation. Despite numerous small studies, the value of ATIII replacement therapy in patients with acquired deficiency remains to be demonstrated. PMID:2679070

  11. Acquired antithrombin III deficiency in patients with glomerular proteinuria.

    PubMed

    Thaler, E; Balzar, E; Kopsa, H; Pinggera, W F

    1978-01-01

    Antithrombin III (AT II/III) was determined immunologically and by means of a heparin cofactor assay in plasma samples and 24-hour urine of 15 patients with various degrees of proteinuria, being predominantly of glomerular origin. In urine the AT II/III concentrations were significantly correlated to the concentrations of albumin, plasminogen and IgG. One third of the patients had AT II/III plasma levels below the normal range. The plasma levels showed a significant inverse correlation to the AT II/III and albumin clearance rates. Similarily, the plasminogen concentrations in plasma were decreased in two thirds of the patients, being inversely correlated to the renal plasminogen clearance values. It is proposed that AT II/III deficiency in the nephrotic syndrome is an important pathogenetic factor in venous thrombosis. PMID:689489

  12. 21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues. Alpha-1-antichymotrypsin helps protect tissues against proteolytic (protein-splitting) enzymes released during...

  13. Microheterogeneity of antithrombin III: effect of single amino acid substitutions and relationship with functional abnormalities.

    PubMed

    De Stefano, V; Leone, G; Mastrangelo, S; Lane, D A; Girolami, A; de Moerloose, P; Sas, G; Abildgaard, U; Blajchman, M; Rodeghiero, F

    1994-02-01

    Microheterogeneity of antithrombin III (AT-III) was investigated by crossed immunoelectrofocusing (CIEF) on eleven molecular variants. A normal pattern was found in five variants while two different abnormal CIEF patterns were found in the other four and two variants, respectively. Point mutations causing a major pI change (exceeding 4.0) of the amino acid substituted lead to alterations in the overall microheterogeneity. The variants thus substituted share a first type of abnormal CIEF pattern with alterations throughout the pH range, regardless of the location of the mutation (reactive site and adjacent regions or heparin binding region). Minor amino acid pI changes in these regions do not alter the AT-III overall microheterogeneity, whatever the resulting functional defect. However, if the mutation is placed in the region around positions 404 or 429, then even minor changes of the amino acid pI seem able to alter the overall charge, leading to a second type of abnormal CIEF pattern with the main alteration at pH 4.8-4.6. Neuraminidase treatment leads to disappearance of microheterogeneity except for the variants with the Arg393 to Cys substitution. Addition of thrombin induces CIEF modifications specifically related to the functional defect. A normal formation of thrombin-antithrombin complexes induces a shift towards the more acid pH range, whereas in the variants substituted at the reactive site the CIEF pattern is substantially unaffected by thrombin; variants substituted at positions 382-384 show a maximal thrombin-induced increase of the isoforms at pI 4.8-4.6. Therefore mutant antithrombins with different functional abnormalities but sharing a common CIEF pattern were well distinguished.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:8180341

  14. Decrease in antithrombin III and prothrombin serum levels contribute to coagulation disorders during leptospirosis.

    PubMed

    Fernandes, Luis G V; Filho, Antonio F S; Souza, Gisele O; Vasconcellos, Silvio A; Romero, Eliete C; Nascimento, Ana L T O

    2016-08-01

    Pathogenic bacteria of the genus Leptospira are the causative agent of leptospirosis, an emergent infectious disease that affects humans and animals worldwide. Severe forms of the disease in humans include jaundice, multiple organ failure and intense haemorrhage. Up to now, mechanisms associated with the haemorrhage foci are poorly understood. We report in this work that, despite the low levels of antithrombin III in convalescent human serum samples, virulent, culture-attenuated and saprophyte strains of Leptospira are unable to bind and/or degrade this thrombin inhibitor, suggesting an indirect mechanism of pathogenesis. Lower levels of prothrombin were found in serum samples at the onset and convalescent phase of the disease when compared to normal human sera. The concomitant decreased levels of antithrombin III and prothrombin suggest a process of stimulated coagulation, which is corroborated by the increase of prothrombin fragment F1+2 in the serum samples. Data obtained with hamsters experimentally infected with virulent Leptospira interrogans serovars Kennewicki and Canicola strongly point out that haemorrhage is correlated with decreased levels of thrombin inhibitors and prothrombin. Activated coagulation might lead to an overconsumption of coagulation factors ultimately leading to bleeding and organ failure. PMID:27260249

  15. Antithrombin III and fibrinogen degradation product (fragment E) in diabetic nephropathy.

    PubMed

    Chan, V; Yeung, C K; Chan, T K

    1982-06-01

    Plasma antithrombin III (AtIII), serum fragment E (FgE) and urine AtIII and FgE were measured in 25 diabetic patients with proteinuria above 1 g per day and compared to that in 25 patients with non-diabetic nephropathy, matched for the degree of proteinuria. Plasma AtIII concentrations were normal in both groups but FgE concentrations were increased. The level of plasma AtIII was directly related to HbA1 concentrations in the diabetics. For the same degree of proteinuria, the diabetic patients lost more AtIII and FgE in the urine. Urine AtIII was found to be mostly bound to activated procoagulants. Both urine AtIII and urine FgE correlated inversely with creatinine clearance. It was concluded that intraglomerular thrombosis probably contributes to the deteriorating renal function in diabetic nephropathy and is reflected in the concentrations of urine AtIII and FgE. PMID:7085916

  16. Antithrombin III and fibrinogen degradation product (fragment E) in diabetic nephropathy.

    PubMed Central

    Chan, V; Yeung, C K; Chan, T K

    1982-01-01

    Plasma antithrombin III (AtIII), serum fragment E (FgE) and urine AtIII and FgE were measured in 25 diabetic patients with proteinuria above 1 g per day and compared to that in 25 patients with non-diabetic nephropathy, matched for the degree of proteinuria. Plasma AtIII concentrations were normal in both groups but FgE concentrations were increased. The level of plasma AtIII was directly related to HbA1 concentrations in the diabetics. For the same degree of proteinuria, the diabetic patients lost more AtIII and FgE in the urine. Urine AtIII was found to be mostly bound to activated procoagulants. Both urine AtIII and urine FgE correlated inversely with creatinine clearance. It was concluded that intraglomerular thrombosis probably contributes to the deteriorating renal function in diabetic nephropathy and is reflected in the concentrations of urine AtIII and FgE. Images PMID:7085916

  17. Antithrombin III-beta associates more readily than antithrombin III-alpha with uninjured and de-endothelialized aortic wall in vitro and in vivo

    SciTech Connect

    Witmer, M.R.; Hatton, M.W. )

    1991-05-01

    The properties of two isoforms, alpha and beta, of rabbit antithrombin III (ATIII) were compared in the presence of undamaged or de-endothelialized rabbit aortic wall. Similar quantities of ATIII-alpha and ATIII-beta bound to and rapidly saturated the endothelium in vitro, but the rate of transendothelial passage of ATIII-beta exceeded that of ATIII-alpha by 22%. Furthermore, ATIII-beta was adsorbed approximately twice as rapidly as ATIII-alpha by the subendothelium of the de-endothelialized aorta. Binding of both isoforms was decreased (ATIII-beta more than ATIII-alpha) by pretreating the subendothelial surface with heparitinase. Also, subendothelium-bound ATIII-beta was desorbed more readily than bound ATIII-alpha by thrombin. In vivo, the rate of uptake of iodine-131-labeled ATIII-beta from the circulation by the aortic wall and the major organs was 30-50% faster than that of iodine-125-labeled ATIII-alpha. In contrast, the uptake of {sup 131}I-ATIII-beta by the de-endothelialized aorta in vivo was three times faster than that of {sup 125}I-ATIII-alpha. By these criteria, ATIII-beta is the more active of the two isoforms. We surmise that plasma and, consequently, vessel wall levels of ATIII-beta may be vital for controlling thrombogenic events caused by injury to the vascular wall.

  18. Low molecular weight heparin restores antithrombin III activity from hyperglycemia induced alterations.

    PubMed

    Ceriello, A; Marchi, E; Palazzni, E; Quatraro, A; Giugliano, D

    1990-01-01

    Alteration of antithrombin III (ATIII) activity, glycemia level dependent, exists in diabetes mellitus. In this study the ability of a low molecular weight heparin (LMWH) (Fluxum, Alfa-Wassermann S.p.A., Bologna, Italy), as well as unfractioned héparin, to preserve ATIII activity from glucose-induced alterations, both in vitro and in vivo, is reported. The subcutaneous and intravenous LMWH and heparin administration increases basal depressed ATIII activity in diabetic patients. Heparin shows an equivalent effect on both anti-IIa and anti-Xa activity of ATIII, while LMWH is more effective in preserving the anti-Xa activity. Similarity, heparin preserves ATIII activity from hyperglycemia-induced alterations, during hyperglycemic clamp, and LMWH infusion is able to preserve a significant amount of anti-Xa activity from glucose-induced alterations. Since diabetic patients show a high incidence of thrombotic accidents, LMWH appears to be a promising innovation for the prevention of diabetic thrombophylia. PMID:2196192

  19. 21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Alpha-1-antichymotrypsin immunological test system. 866.5080 Section 866.5080 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues....

  20. 21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Alpha-1-antichymotrypsin immunological test system. 866.5080 Section 866.5080 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues....

  1. 21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Alpha-1-antichymotrypsin immunological test system. 866.5080 Section 866.5080 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues....

  2. 21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Alpha-1-antichymotrypsin immunological test system. 866.5080 Section 866.5080 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues....

  3. Heparin binding domain of antithrombin III: Characterization using a synthetic peptide directed polyclonal antibody

    SciTech Connect

    Smith, J.W.; Dey, B.; Knauer, D.J. )

    1990-09-25

    Antithrombin III (ATIII) is a plasma-borne serine protease inhibitor that apparently forms covalent complexes with thrombin. The interaction between ATIII and thrombin is enhanced several thousandfold by the glycosaminoglycan, heparin. The authors have previously proposed that the heparin binding site of ATIII residues within a region extending from amino acid residues 114-156. Computer-assisted analysis of this region revealed the presence of a 22 amino acid domain (residues 124-145), part of which shows a strong potential for the formation of an amphipathic helix: hydrophobic on one face and highly positively charged on the other. In the presence studies, polyclonal antisera were generated against a synthetic peptide corresponding to residues 124-145 in native human ATIII. Affinity-purified IgG from these antisera, as well as monovalent Fab's derived from them, specifically blocked the binding of heparin to ATIII. Additionally, occupancy of the heparin binding site by these same monovalent and bivalent IgG's at least partially substituted for heparin, accelerating linkage formation between ATIII and thrombin. These results provide the first immunological evidence that region 124-145 is directly involved in the binding of heparin to ATIII and that an antibody-induced conformational change within this region can mediate ATIII activation.

  4. Targeted mutagenesis of zebrafish antithrombin III triggers disseminated intravascular coagulation and thrombosis, revealing insight into function

    PubMed Central

    Liu, Yang; Kretz, Colin A.; Maeder, Morgan L.; Richter, Catherine E.; Tsao, Philip; Vo, Andy H.; Huarng, Michael C.; Rode, Thomas; Hu, Zhilian; Mehra, Rohit; Olson, Steven T.; Joung, J. Keith

    2014-01-01

    Pathologic blood clotting is a leading cause of morbidity and mortality in the developed world, underlying deep vein thrombosis, myocardial infarction, and stroke. Genetic predisposition to thrombosis is still poorly understood, and we hypothesize that there are many additional risk alleles and modifying factors remaining to be discovered. Mammalian models have contributed to our understanding of thrombosis, but are low throughput and costly. We have turned to the zebrafish, a tool for high-throughput genetic analysis. Using zinc finger nucleases, we show that disruption of the zebrafish antithrombin III (at3) locus results in spontaneous venous thrombosis in larvae. Although homozygous mutants survive into early adulthood, they eventually succumb to massive intracardiac thrombosis. Characterization of null fish revealed disseminated intravascular coagulation in larvae secondary to unopposed thrombin activity and fibrinogen consumption, which could be rescued by both human and zebrafish at3 complementary DNAs. Mutation of the human AT3-reactive center loop abolished the ability to rescue, but the heparin-binding site was dispensable. These results demonstrate overall conservation of AT3 function in zebrafish, but reveal developmental variances in the ability to tolerate excessive clot formation. The accessibility of early zebrafish development will provide unique methods for dissection of the underlying mechanisms of thrombosis. PMID:24782510

  5. Formation of thrombin-antithrombin III complex using polyamide and hemophan dialyzers.

    PubMed

    Schultze, G; Hollmann, S; Sinah, P

    1992-06-01

    The recently developed ELISA for the thrombin-antithrombin III complex (TAT) is a sensitive, specific, and simplified means of detecting intravascular coagulation. For further evaluation of the thrombogenicity of a polyamide (P) and a Hemophan (H) hollow-fibre dialyzer a cross-over study was done in ten stable patients on maintenance hemodialysis. At the same doses of heparin (mean bolus of 30 U/kg bw and maintenance doses of 86 U/kg bw), thrombin time and partial thromboplastin time were significantly lower using H. At the end of dialysis TAT was significantly higher in H (mean +/- SEM before HD 3.57 +/- .56, at 240 min 14.9 +/- 6.5 ng/ml, p less than 0.05, Wilcoxon-test) than in P (before HD 4.36 +/- .98, at 240 min 8.95 +/- 3.0 ng/ml, p less than 0.05 H 240 vs. P 240, Wilcoxon-test). Visible clotting was more pronounced in the H filter. Among other favourable features of blood compatibility the polyamide/polyvinylpyrrolidone copolymer with a hydrophilic/hydrophobic microdomain structure has less thrombogenicity. The modified cellulosic membrane H has advantages in complement activation and leukocyte depression, but thrombogenicity seems less favourable since the incorporated diethyl-amino-ethyl groups with their positive charge bind and inactivate negatively charged heparin. PMID:1639530

  6. Structural investigation of the alpha-1-antichymotrypsin: prostate-specific antigen complex by comparative model building.

    PubMed Central

    Villoutreix, B. O.; Lilja, H.; Pettersson, K.; Lövgren, T.; Teleman, O.

    1996-01-01

    Prostate-specific antigen (PSA), produced by prostate cells, provides an excellent serum marker for prostate cancer. It belongs to the human kallikrein family of enzymes, a second prostate-derived member of which is human glandular kallikrein-1 (hK2). Active PSA and hK2 are both 237-residue kallikrein-like proteases, based on sequence homology. An hK2 model structure based on the serine protease fold is presented and compared to PSA and six other serine proteases in order to analyze in depth the role of the surface-accessible loops surrounding the active site. The results show that PSA and hK2 share extensive structural similarity and that most amino acid replacements are centered on the loops surrounding the active site. Furthermore, the electrostatic potential surfaces are very similar for PSA and hK2. PSA interacts with at least two serine protease inhibitors (serpins): alpha-1-antichymotrypsin (ACT) and protein C inhibitor (PCI). Three-dimensional model structures of the uncleaved ACT molecule were developed based upon the recent X-ray structure of uncleaved antithrombin. The serpin was docked both to PSA and hK2. Amino acid replacements and electrostatic complementarities indicate that the overall orientation of the proteins in these complexes is reasonable. In order to investigate PSA's heparin interaction sites, electrostatic computations were carried out on PSA, hK2, protein C, ACT, and PCI. Two heparin binding sites are suggested on the PSA surface and could explain the enhanced complex formation between PSA and PCI, while inhibiting the formation of the ACT-PSA complex, PSA, hK2, and their preliminary complexes with ACT should facilitate the understanding and prediction of structural and functional properties for these important proteins also with respect to prostate diseases. PMID:8732755

  7. Resolution of preoperative portal vein thrombosis after administration of antithrombin III in living donor liver transplantation: case report.

    PubMed

    Imai, H; Egawa, H; Kajiwara, M; Nakajima, A; Ogura, Y; Hatano, E; Ueda, M; Kawaguchi, Y; Kaido, T; Takada, Y; Uemoto, S

    2009-11-01

    A 59-year-old man with hepatitis C virus-associated liver cirrhosis was transferred to our hospital to undergo living donor liver transplantation. Coagulation was impaired (prothrombin time [International Normalized Ratio], 3.27), and antithrombin III (AT-III) activity was 23% (normal, 87%-115%). Contrast-enhanced computed tomography scans revealed portal vein thrombosis (PVT) from the junction between the splenic and superior mesenteric vein to the porta hepatica; the portal vein was completely obstructed (PVT). To prevent further development of PVT, 1500 U of AT-III was administered for 3 days, elevating the AT-III activity to 50%. A contrast-enhanced computed tomography scan obtained 9 days after AT-III administration showed resolution of PVT. Living donor liver transplantation was safely performed without portal vein grafting. Thus, a low AT-III concentration may have an important role in the pathogenesis of PVT in patients with cirrhosis. PMID:19917415

  8. Pivotal Role for α1-Antichymotrypsin in Skin Repair*

    PubMed Central

    Hoffmann, Daniel C.; Textoris, Christine; Oehme, Felix; Klaassen, Tobias; Goppelt, Andreas; Römer, Axel; Fugmann, Burkhard; Davidson, Jeffrey M.; Werner, Sabine; Krieg, Thomas; Eming, Sabine A.

    2011-01-01

    α1-Antichymotrypsin (α1-ACT) is a specific inhibitor of leukocyte-derived chymotrypsin-like proteases with largely unknown functions in tissue repair. By examining human and murine skin wounds, we showed that following mechanical injury the physiological repair response is associated with an acute phase response of α1-ACT and the mouse homologue Spi-2, respectively. In both species, attenuated α1-ACT/Spi-2 activity and gene expression at the local wound site was associated with severe wound healing defects. Topical application of recombinant α1-ACT to wounds of diabetic mice rescued the impaired healing phenotype. LC-MS analysis of α1-ACT cleavage fragments identified a novel cleavage site within the reactive center loop and showed that neutrophil elastase was the predominant protease involved in unusual α1-ACT cleavage and inactivation in nonhealing human wounds. These results reveal critical functions for locally acting α1-ACT in the acute phase response following skin injury, provide mechanistic insight into its function during the repair response, and raise novel perspectives for its potential therapeutic value in inflammation-mediated tissue damage. PMID:21693707

  9. Pivotal role for alpha1-antichymotrypsin in skin repair.

    PubMed

    Hoffmann, Daniel C; Textoris, Christine; Oehme, Felix; Klaassen, Tobias; Goppelt, Andreas; Römer, Axel; Fugmann, Burkhard; Davidson, Jeffrey M; Werner, Sabine; Krieg, Thomas; Eming, Sabine A

    2011-08-19

    α1-Antichymotrypsin (α1-ACT) is a specific inhibitor of leukocyte-derived chymotrypsin-like proteases with largely unknown functions in tissue repair. By examining human and murine skin wounds, we showed that following mechanical injury the physiological repair response is associated with an acute phase response of α1-ACT and the mouse homologue Spi-2, respectively. In both species, attenuated α1-ACT/Spi-2 activity and gene expression at the local wound site was associated with severe wound healing defects. Topical application of recombinant α1-ACT to wounds of diabetic mice rescued the impaired healing phenotype. LC-MS analysis of α1-ACT cleavage fragments identified a novel cleavage site within the reactive center loop and showed that neutrophil elastase was the predominant protease involved in unusual α1-ACT cleavage and inactivation in nonhealing human wounds. These results reveal critical functions for locally acting α1-ACT in the acute phase response following skin injury, provide mechanistic insight into its function during the repair response, and raise novel perspectives for its potential therapeutic value in inflammation-mediated tissue damage. PMID:21693707

  10. Antithrombin Test

    MedlinePlus

    ... deficiency. (For more about excessive clotting (such as deep vein thrombosis, DVT) and antithrombin deficiency, see the " ... affected person may bleed and/or clot. DVT (deep vein thrombosis – a blood clot usually in a ...

  11. Antithrombin III and its interaction with heparin. Comparison of the human, bovine, and porcine proteins by /sup 1/H NMR spectroscopy

    SciTech Connect

    Gettins, P.

    1987-03-10

    /sup 1/H NMR has been used to characterize and compare the structures of antithrombin III from human, bovine, and porcine plasma as well as to investigate the interactions of each of these proteins with heparin fragments of defined length. The amino acid compositions of the three proteins are very similar, which is reflected in the gross features of their /sup 1/H NMR spectra. Human antithrombin III has five histidine residues, bovine has six, and porcine has five. The C(2) proton from each of these residues gives a narrow resonance and titrates with pH; the pK/sub a/'s are in the range 5.15-7.25. It is concluded that all histidines in each protein are surface residues with considerable independent mobility. The carbohydrate chains in each protein also give sharp resonances consistent with a surface location and motional flexibility. The /sup 1/H spectra are sensitive to heparin binding. Although heparin resonances obscure protein resonances in the region 3.2-6.0 ppm, difference spectra between antithrombin III with and without heparin show clear perturbation of a small number of aromatic and aliphatic protein protons. For human antithrombin III, it was shown that heparin fragments 8, 10, and 16 sugar residues in length result in almost identical perturbations to the protein. In contrast, tetrasaccharide results in fewer perturbations. Significantly, intact high molecular weight heparin causes the same spectral perturbations as the 16-residue fragment. These data are discussed in terms of requirements for heparin binding.

  12. Effect of the oral contraceptive pill on protein S and antithrombin-III levels in Malaysian women.

    PubMed

    Wong, K K; Ng, S C; Koong, P L

    Studies focusing on the relationship between oral contraceptive (OC) usage and occurrence of thromboembolism have been conducted for over 3 decades. Those studies centered on the effects OC use has on blood proteins and on measurable physiological changes that occurred in women with venous thrombosis. This article reports the findings of a study that investigated the effects of OC use on the levels of the anticoagulants antithrombin-III (AT-III), protein C (PC), and protein S (PS) in a group of Asian women. Previous studies had mostly been based on Caucasian women. Of the 21 women studied, 16 were Malaysian, 3 were Chinese, and 2 were Indian. Low-dose OCs containing 30 mcg of ethinyl estradiol and 150 mcg of either desogestrel or levonorgestrel were used. Blood was tested before OC use and 3 and 6 months after starting OC use. Levels of AT-III and PS were measured using the Laurell rocket immunoelectrophoresis technique. Statistical analysis was performed using the paired Student's t-test and an analysis of variance test. No statistically significant differences were found for the mean levels of AT-III and total PS when comparing the pre-OC with the 3- and 6-month post-OC values. Earlier studies based mostly on Caucasian women have reported lower levels of both total PS and free PS in OC users. PMID:12288974

  13. Developmental expression of chicken antithrombin III is regulated by increased RNA abundance and intracellular processing.

    PubMed

    Amrani, D L; Rosenberg, J; Samad, F; Bergtrom, G; Banfield, D K

    1993-01-23

    We isolated and sequenced a 432 bp cDNA to cAT-III, that encoded 115 nucleotides of 5' untranslated sequence, a 17 amino acid long signal peptide and residues 1-88 of the mature protein, and used it to prepare a probe for measuring and correlating the developmental changes of steady-state cAT-III mRNA levels with known changes in antigen levels. Densitometric analysis of nuclease protection (n = 2), Northern blot (n = 4), and slot blots (n = 3) of total RNA from chick livers of 16-day-old embryos to 6-day-old chicks showed a 2.6 +/- 0.5-fold increase in steady-state cAT-III mRNA levels. Assay of functional mRNA levels by in vitro translation of poly(A)+ RNA and specific immunoprecipitation of 35S-Met-labelled cAT-III was comparable to RNA analysis (16-day-old embryos vs. 10-day-old hatchlings). We evaluated whether there were developmental differences in post-translational secretion which may also contribute to the regulation of the circulating level of this protein. Pulse-chase studies of freshly-isolated hepatocytes from 16-day-old embryos and 10-day-old hatchlings maintained in suspension demonstrated a approx. 5.0-5.5-fold increase in cAT-III levels at steady-state secretion. The above findings indicate that changes in circulating cAT-III levels during late embryonic development are primarily due to increased abundance of cAT-III mRNA. In addition, we postulate that post-translational intracellular processing may account for further differences in circulating protein levels. PMID:8424948

  14. Use of quantitative affinity chromatography for characterizing high-affinity interactions: binding of heparin to antithrombin III.

    PubMed

    Hogg, P J; Jackson, C M; Winzor, D J

    1991-02-01

    The versatility of quantitative affinity chromatography (QAC) for evaluating the binding of macromolecular ligands to macromolecular acceptors has been increased substantially as a result of the derivation of the equations which describe the partitioning of acceptor between matrix-bound and soluble forms in terms of total, rather than free, ligand concentrations. In addition to simplifying the performance of the binding experiments, this development makes possible the application of the technique to systems characterized by affinities higher than those previously amenable to investigation by QAC. Addition of an on-line data acquisition system to monitor the concentration of partitioning solute in the liquid phase as a function of time has permitted the adoption of an empirical approach for determining the liquid-phase concentration of acceptor in the system at partition equilibrium, a development which decreases significantly the time required to obtain a complete binding curve by QAC. The application of these new QAC developments is illustrated by the determination of binding constants for the interactions of high-affinity heparin (Mr 20,300) with antithrombin III at three temperatures. Association constants of 8.0 +/- 2.2 x 10(7), 3.4 +/- 0.3 x 10(7), and 1.0 +/- 0.2 x 10(7) M-1 were observed at 15, 25, and 35 degrees C, respectively. The standard enthalpy change of -4.2 +/- 0.6 kcal/mol that is calculated from these data is in good agreement with a reported value obtained from fluorescence quenching measurements. PMID:2035830

  15. Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: complex formation as a mechanism of inactivation

    PubMed Central

    Meyer-Siegler, Katherine L.; Cox, Jacob; Leng, Lin; Bucala, Richard; Vera, Pedro L.

    2009-01-01

    Mounting evidence suggests that the proinflammatory cytokine macrophage migration inhibitory factor (MIF) may serve as an important link between chronic inflammation and carcinogenesis as evidenced by the increase in serum MIF found in patients with various cancers. The present study identifies anti-thrombin III (ATIII) as an endogenous MIF binding protein, which reduces MIF biological activity. Serum MIF in bladder cancer patients (TCC stage II, n=50) was increased when compared to normal patients (n=50), while ATIII-MIF complexes were decreased in bladder cancer patient serum. These data suggest that increased circulating levels of bioactive MIF are present in bladder cancer patient serum. PMID:19762145

  16. Congenital antithrombin III deficiency

    MedlinePlus

    ... LE, Heslop HE, Weitz JI, Anastasi J, eds. Hematology: Basic Principles and Practice . 6th ed. Philadelphia, PA: ... Nanda, MD, Assistant Professor of Medicine, Section of Hematology/Oncology, University of Chicago Medicine, Chicago, IL. Review ...

  17. Purification of proplatelet formation (PPF) stimulating factor: thrombin/antithrombin III complex stimulates PPF of megakaryocytes in vitro and platelet production in vivo.

    PubMed

    Ishida, Y; Yano, K; Ito, T; Shigematus, H; Sasaki, K; Kondo, S; Kuriya, S

    2001-02-01

    In this study, the protein which stimulates proplatelet formation (PPF) of megakaryocytes was purified from normal human plasma using 7 steps procedures. Two different protease inhibitors were identified based on their amino acid sequences, i.e. antithrombin III (AT III) and C1 inhibitor. They were included in high density lipoprotein (HDL). HDL was necessary for AT III to be active in PPF in vitro. The biological effects of the AT III/HDL or thrombin-AT III (TAT)/HDL were studied in vitro. PPF of murine megakaryocytes was stimulated by negative control (BSA) (1.8 +/- 0.3%), AT III (2.0 +/- 0.4%), HDL (1.2 +/- 0.9%), AT III/HDL (14.8 +/- 2.1%) or TAT/HDL (23.3 +/- 3.5%), respectively. TAT/HDL also had a synergistic effect with the mpl ligand, judging by the acetylcholinesterase (AchE) expression of murine megakaryocytes (2.7 fold increase). In vivo subcutaneous administration of AT III alone or TAT for 3 days significantly stimulated thrombocytosis (136% and 144%, respectively, p<0.05) and AT III/HDL showed rapid and further stimulation (150%, p <0.01). These results and the previous studies indicate that megakaryocytopoiesis is regulated by the mpl ligand, while a protease/protease inhibitor complex such as TAT, which is involved in the coagulation cascade associated with platelet consumption, might be one of the regulators in platelet production. PMID:11246559

  18. Is there an association of regulatory region polymorphism in the alpha-1-antichymotrypsin gene with sporadic Alzheimer's disease in the northern Han-Chinese population?

    PubMed

    Yu, Guran; Jia, Jianping

    2010-06-01

    Both invitro and invivo alpha-1-antichymotrypsin (ACT) directly inhibits amyloid beta peptide (Abeta) degradation and promotes Abeta deposition. However, whether the genetic variants in the regulatory region (including the promoter and the two enhancers) of the ACT gene affect susceptibility to Alzheimer's disease (AD) remains controversial. Here, we screened ACT promoter and enhancers in 244 patients with sporadic Alzheimer's disease (SAD) and 205 control patients, both of north Han-Chinese origin. Four single nucleotide polymorphisms (SNP) were found: (i) 11510T/C (rs10145747, named as ACT1); 11496G/A (rs4375593, ACT2); (iii) 11491T/C (rs4508366, ACT3); and (iv) 51G/T (rs1884082, ACT4). Neither individual SNP nor haplotypes were associated with AD onset. We concluded that the effect of the variations in the ACT regulatory region must be very limited, if occurring at all. PMID:20378355

  19. Enhancement by heparin of thrombin-induced antithrombin III proteolysis: its relation to the molecular weight and anticoagulant activity of heparin

    SciTech Connect

    Marciniak, E.; Gora-Maslak, G.

    1982-11-01

    Previous findings indicated that binding of heparin to antithrombin III (AT III) facilitates thrombin-induced proteolysis of the inhibitor. Researchers now studied this property of heparin in regard to its molecular weight and anticoagulant activity. Commercial heparin was resolved on Sephadex G-200 into six fractions of decreasing molecular weight. From each fraction high affinity (HA) heparin was isolated by chromatography on AT III-Sepharose and examined in reaction of alpha-thrombin with a molar excess of /sup 125/I AT III. Proteolysis of the inhibitor was assessed by SDS polyacrylamide gel electrophoresis. In the presence of the HA heparin from 18% to 38% of AT III participating in reaction appeared in the form of inactive 50,000-dalton fragment, as opposed to 7% of AT III fragmented in the absence of heparin. Although the ability to potentiate proteolysis was at its peak in the medium-molecular-size heparin fraction, the amount of degraded inhibitor relative to anticoagulant activity increased with decreasing molecular weight of the polysaccharide. These findings are consistent with the possibility that the ability of bound heparin to facilitate the cleavage of AT III by thrombin is generally less contingent upon secondary characteristics of the polysaccharide than the anticoagulant activity.

  20. No genetic effect of {alpha}{sub 1}-antichymotrypsin in Alzheimer disease

    SciTech Connect

    Haines, J.L.; Pritchard, M.L.; Saunders, A.M.

    1996-04-01

    Alzheimer disease (AD) is the most common neurodegenerative disorder for individuals over the age of 40.AD has a complex etiology, and it is likely that multiple genes, acting independently and/or interacting, affect the risk of developing AD. Several genes involved with AD have been described already, but only the APOE gene on chromosome 19q has been shown to affect the risk of the common late onset form of AD. {alpha}{sub 1}-Antichymotrypsin (AACT) is a major component of the amyloid plaques found in the brains of AD patients, and an allele in its gene has been proposed to increase the risk of developing AD when also associated with the APOE-4 allele. We have examined the role of this AACT polymorphism in a large set of families and sporadic cases, and do not see any effect, either alone or in combination with the APOE-4 allele. 18 refs., 3 tabs.

  1. α1-Antichymotrypsin inactivates staphylococcal cysteine protease in cross-class inhibition.

    PubMed

    Wladyka, Benedykt; Kozik, Agata J; Bukowski, Michal; Rojowska, Anna; Kantyka, Tomasz; Dubin, Grzegorz; Dubin, Adam

    2011-05-01

    Staphylococcal cysteine proteases are implicated as virulence factors in human and avian infections. Human strains of Staphylococcus aureus secrete two cysteine proteases (staphopains A and B), whereas avian strains express staphopain C (ScpA2), which is distinct from both human homologues. Here, we describe probable reasons why the horizontal transfer of a plasmid encoding staphopain C between avian and human strains has never been observed. The human plasma serine protease inhibitor α(1)-antichymotrypsin (ACHT) inhibits ScpA2. Together with the lack of ScpA2 inhibition by chicken plasma, these data may explain the exclusively avian occurrence of ScpA2. We also clarify the mechanistic details of this unusual cross-class inhibition. Analysis of mutated ACHT variants revealed that the cleavage of the Leu383-Ser384 peptide bond results in ScpA2 inhibition, whereas hydrolysis of the preceding peptide bond leads to ACHT inactivation. This evidence is consistent with the suicide-substrate-like mechanism of inhibition. PMID:21296644

  2. Alpha1-antichymotrypsin activity correlates with and may modulate matrix metalloproteinase-9 in human acute wounds.

    PubMed

    Reiss, Matthew J; Han, Yuan-Ping; Garner, Warren L

    2009-01-01

    Matrix metalloproteinase-9 (MMP-9) plays a central role in many physiologic processes including acute and the chronic wounds. MMP-9 is not routinely expressed in healthy tissues but is promptly expressed as a proenzyme and converted into active enzyme after tissue injury. The mechanisms involved, including the activators and inhibitors for this enzyme in human tissue remain largely obscure. We recently identified alpha1-antichymotrypsin (alpha1-ACT), an acute phase factor, as a potent inhibitor controlling activation of pro-MMP-9 by human skin. The aim of this study is to establish the clinical relevance of the inhibitor in cutaneous wound healing. Fluids from acute burn blisters and conditioned media from skin explants of burn patients were analyzed. We observed that the presence pro-MMP-9 and its activation correlated with the proximity to and degree of injury. Early after trauma, massive levels of wound alpha1-ACT were associated with an absence of pro-MMP-9 activation. Conversely, the active MMP-9 occurs simultaneously with inactivation of alpha1-ACT. Our results suggest a role for alpha1-ACT as a physiologic inhibitor of MMP-9 activation in human wound healing. PMID:19660051

  3. alpha 1-Antichymotrypsin is the human plasma inhibitor of macrophage ectoenzymes that cleave pro-macrophage stimulating protein.

    PubMed

    Skeel, A; Leonard, E J

    2001-06-15

    Macrophage stimulating protein (MSP) is secreted as 78-kDa single chain pro-MSP, which is converted to biologically active, disulfide-linked alphabeta chain MSP by cleavage at Arg(483)-Val(484). Murine resident peritoneal macrophages have two cell surface proteolytic activities that cleave pro-MSP. One is a pro-MSP convertase, which cleaves pro-MSP to active MSP; the other degrades pro-MSP. The degrading protease is inhibited by soybean trypsin inhibitor or by low concentrations of blood plasma, which allows the convertase to cleave pro-MSP to MSP. Using pro-MSP cleavage as the assay, we purified the inhibitor from human plasma. The bulk of the plasma protein was removed by salting out and by isoelectric precipitation of albumin. Highly purified inhibitor was then obtained in three steps: dye-ligand binding and elution, ion exchange chromatography, and high performance liquid chromatography gel filtration. After SDS-polyacrylamide gel electrophoresis and transfer to a polyvinylidene membrane, N-terminal sequencing of the product identified it as alpha(1)-antichymotrypsin. The mean concentration of alpha(1)-antichymotrypsin in human plasma is 7 micrometer. At this concentration, alpha(1)-antichymotrypsin inhibits both macrophage enzymes. A concentration of 0.4 micrometer, which is in the expected concentration range in extracellular fluid, preferentially inhibits the degrading enzyme, which allows for cleavage to active MSP by the pro-MSP convertase. PMID:11274154

  4. In Vivo Anti-HIV Activity of the Heparin-Activated Serine Protease Inhibitor Antithrombin III Encapsulated in Lymph-Targeting Immunoliposomes

    PubMed Central

    Asmal, Mohammed; Whitney, James B.; Luedemann, Corinne; Carville, Angela; Steen, Robert; Letvin, Norman L.; Geiben-Lynn, Ralf

    2012-01-01

    Endogenous serine protease inhibitors (serpins) are anti-inflammatory mediators with multiple biologic functions. Several serpins have been reported to modulate HIV pathogenesis, or exhibit potent anti-HIV activity in vitro, but the efficacy of serpins as therapeutic agents for HIV in vivo has not yet been demonstrated. In the present study, we show that heparin-activated antithrombin III (hep-ATIII), a member of the serpin family, significantly inhibits lentiviral replication in a non-human primate model. We further demonstrate greater than one log10 reduction in plasma viremia in the nonhuman primate system by loading of hep-ATIII into anti-HLA-DR immunoliposomes, which target tissue reservoirs of viral replication. We also demonstrate the utility of hep-ATIIII as a potential salvage agent for HIV strains resistant to standard anti-retroviral treatment. Finally, we applied gene-expression arrays to analyze hep-ATIII-induced host cell interactomes and found that downstream of hep-ATIII, two independent gene networks were modulated by host factors prostaglandin synthetase-2, ERK1/2 and NFκB. Ultimately, understanding how serpins, such as hep-ATIII, regulate host responses during HIV infection may reveal new avenues for therapeutic intervention. PMID:23133620

  5. Investigating changes in the gas-phase conformation of Antithrombin III upon binding of Arixtra using traveling wave ion mobility spectrometry (TWIMS).

    PubMed

    Zhao, Yuejie; Singh, Arunima; Li, Lingyun; Linhardt, Robert J; Xu, Yongmei; Liu, Jian; Woods, Robert J; Amster, I Jonathan

    2015-10-21

    We validate the utility of ion mobility to measure protein conformational changes induced by the binding of glycosaminoglycan ligands, using the well characterized system of Antithrombin III (ATIII) and Arixtra, a pharmaceutical agent with heparin (Hp) activity. Heparin has been used as a therapeutic anticoagulant drug for several decades through its interaction with ATIII, a serine protease inhibitor that plays a central role in the blood coagulation cascade. This interaction induces conformational changes within ATIII that dramatically enhance the ATIII-mediated inhibition rate. Arixtra is the smallest synthetic Hp containing the specific pentasaccharide sequence required to bind with ATIII. Here we report the first travelling wave ion mobility mass spectrometry (TWIMS) investigation of the conformational changes in ATIII induced by its interaction with Arixtra. Native electrospray ionization mass spectrometry allowed the gentle transfer of the native topology of ATIII and ATIII-Arixtra complex. IM measurements of ATIII and ATIII-Arixtra complex showed a single structure, with well-defined collisional cross section (CCS) values. An average 3.6% increase in CCS of ATIII occurred as a result of its interaction with Arixtra, which agrees closely with the theoretical estimation of the change in CCS based on protein crystal structures. A comparison of the binding behavior of ATIII under both denaturing and non-denaturing conditions confirmed the significance of a folded tertiary structure of ATIII for its biological activity. A Hp oligosaccharide whose structure is similar to Arixtra but missing the 3-O sulfo group on the central glucosamine residue showed a dramatic decrease in binding affinity towards ATIII, but no change in the mobility behavior of the complex, consistent with prior studies that suggested that 3-O sulfation affects the equilibrium constant for binding to ATIII, but not the mode of interaction. In contrast, nonspecific binding by a Hp

  6. Investigating changes in the gas-phase conformation of Antithrombin III upon binding of Arixtra using traveling wave ion mobility spectrometry (TWIMS)

    PubMed Central

    Zhao, Yuejie; Singh, Arunima; Li, Lingyun; Linhardt, Robert J.; Xu, Yongmei; Liu, Jian; Woods, Robert J.

    2015-01-01

    We validate the utility of ion mobility to measure protein conformational changes induced by the binding of glycosaminoglycan ligands, using the well characterized system of Antithrombin III (ATIII) and Arixtra, a pharmaceutical agent with heparin (Hp) activity. Heparin has been used as a therapeutic anticoagulant drug for several decades through its interaction with ATIII, a serine protease inhibitor that plays a central role in the blood coagulation cascade. This interaction induces conformational changes within ATIII that dramatically enhance the ATIII-mediated inhibition rate. Arixtra is the smallest synthetic Hp containing the specific pentasaccharide sequence required to bind with ATIII. Here we report the first travelling wave ion mobility mass spectrometry (TWIMS) investigation of the conformational changes in ATIII induced by its interaction with Arixtra. Native electrospray ionization mass spectrometry allowed the gentle transfer of the native topology of ATIII and ATIII–Arixtra complex. IM measurements of ATIII and ATIII–Arixtra complex showed a single structure, with well-defined collisional cross section (CCS) values. An average 3.6% increase in CCS of ATIII occurred as a result of its interaction with Arixtra, which agrees closely with the theoretical estimation of the change in CCS based on protein crystal structures. A comparison of the binding behavior of ATIII under both denaturing and non-denaturing conditions confirmed the significance of a folded tertiary structure of ATIII for its biological activity. A Hp oligosaccharide whose structure is similar to Arixtra but missing the 3-O sulfo group on the central glucosamine residue showed a dramatic decrease in binding affinity towards ATIII, but no change in the mobility behavior of the complex, consistent with prior studies that suggested that 3-O sulfation affects the equilibrium constant for binding to ATIII, but not the mode of interaction. In contrast, nonspecific binding by a Hp

  7. Time-resolved fluorescence imaging (TRFI) for direct immunofluorescence of PSA and alpha-1-antichymotrypsin in prostatic tissue sections.

    PubMed

    Bjartell, A; Siivola, P; Hulkko, S; Pettersson, K; Rundt, K; Lilja, H; Lövgren, T

    1999-05-01

    We have developed a direct immunofluorescence technique utilising chelates of the lanthanide ions europium and terbium conjugated to monoclonal IgGs (Mabs) against prostate-specific antigen (PSA) and alpha-1-antichymotrypsin (ACT) for the detection and quantification on the same tissue section. Strong signals without disturbance from tissue autofluorescence were demonstrated in paraffin sections of ten benign and six malignant prostate tissue specimens. The signal intensity increased linearly with the amount of labelled Mab until epitope saturation began. The highest concentrations of bound IgG in tissue sections were 27.3 fmol/pixel for ACT and 7.2 for PSA. Time-resolved fluorescence imaging (TRFI) offers an attractive method for histochemical studies based on specific and quantitative detection of fluorescent lanthanide chelates. PMID:12496823

  8. Antithrombin deficiency in pregnancy.

    PubMed

    Durai, Shivani; Tan, Lay Kok; Lim, Serene

    2016-01-01

    We present a case of a 39-year-old, gravida 3 para 2, Chinese female with a history of inherited type 1 Antithrombin deficiency and multiple prior episodes of venous thromboembolism. She presented at 29+4 weeks' gestation with severe pre-eclampsia complicated by haemolysis, elevated liver enzymes and low platelet (HELLP) syndrome. She subsequently underwent an emergency caesarean section for non-reassuring fetal status, which was complicated by postpartum haemorrhage secondary to uterine atony, requiring a B-Lynch suture intraoperatively. PMID:27207982

  9. Association between alpha-1 antichymotrypsin gene A/T polymorphism and primary intracerebral hemorrhage: a meta-analysis.

    PubMed

    Ye, Zusen; Ye, Qiang; Shao, Bei; He, Jincai; Zhu, Zhenguo; Cheng, Jianhua; Chen, Yanyan; Chen, Siyan; Huang, Xiaoya

    2015-01-01

    The present study is to use meta-analysis to explain the association between alpha-1 antichymotrypsin (ACT) gene A/T polymorphism and the risk of primary intracerebral hemorrhage (PICH). Relevant studies before 1 June 2015 were identified by searching PubMed, Cochrane database and Science Citation Index Expanded (SCIE), and the references of retrieved articles. Pooled odds ratios (ORs) with corresponding 95% confidence intervals (95% CIs) were used to assess the strength of the association. Five independent publications, with 774 PICH cases and 940 controls, were included. There was no statistical evidence of association between ACT polymorphism and PICH risk under all genetic models in overall estimates (allele model: OR = 1.01, 95% CI = 0.80-1.28; heterozygote model: OR = 0.93, 95% CI = 0.60-1.45; homozygote model: OR = 1.03, 95% CI = 0.59-1.80; dominant model: OR = 0.97, 95% CI = 0.65-1.46; recessive model: OR = 1.06, 95% CI = 0.72-1.57). No association was found in subgroup analysis based on ethnicity, Hardy-Weinberg equilibrium, location of hematoma and blood pressure. Sensitivity analysis suggested that the combined results were stable and reliable. No significant publication bias was found by Begg's test and Egger's regression test. The results of our meta-analysis indicate that ACT polymorphism is unlikely to contribute to PICH susceptibility. PMID:26885003

  10. Association between alpha-1 antichymotrypsin gene A/T polymorphism and primary intracerebral hemorrhage: a meta-analysis

    PubMed Central

    Ye, Zusen; Ye, Qiang; Shao, Bei; He, Jincai; Zhu, Zhenguo; Cheng, Jianhua; Chen, Yanyan; Chen, Siyan; Huang, Xiaoya

    2015-01-01

    The present study is to use meta-analysis to explain the association between alpha-1 antichymotrypsin (ACT) gene A/T polymorphism and the risk of primary intracerebral hemorrhage (PICH). Relevant studies before 1 June 2015 were identified by searching PubMed, Cochrane database and Science Citation Index Expanded (SCIE), and the references of retrieved articles. Pooled odds ratios (ORs) with corresponding 95% confidence intervals (95% CIs) were used to assess the strength of the association. Five independent publications, with 774 PICH cases and 940 controls, were included. There was no statistical evidence of association between ACT polymorphism and PICH risk under all genetic models in overall estimates (allele model: OR = 1.01, 95% CI = 0.80-1.28; heterozygote model: OR = 0.93, 95% CI = 0.60-1.45; homozygote model: OR = 1.03, 95% CI = 0.59-1.80; dominant model: OR = 0.97, 95% CI = 0.65-1.46; recessive model: OR = 1.06, 95% CI = 0.72-1.57). No association was found in subgroup analysis based on ethnicity, Hardy-Weinberg equilibrium, location of hematoma and blood pressure. Sensitivity analysis suggested that the combined results were stable and reliable. No significant publication bias was found by Begg’s test and Egger’s regression test. The results of our meta-analysis indicate that ACT polymorphism is unlikely to contribute to PICH susceptibility. PMID:26885003

  11. Alzheimer disease A68 proteins injected into rat brain induce codeposits of beta-amyloid, ubiquitin, and alpha 1-antichymotrypsin.

    PubMed Central

    Shin, R W; Bramblett, G T; Lee, V M; Trojanowski, J Q

    1993-01-01

    Aberrantly phosphorylated tau proteins (i.e., A68 or PHF-tau) and beta-amyloid or A4 (beta A4) peptides are major components of pathologic lesions in Alzheimer disease (AD). Although A68 and beta A4 colocalize in AD neurofibrillary tangles (NFTs) and amyloid-rich senile plaques (SPs), the mechanisms leading to the convergence of A68, beta A4, and other proteins in the same AD lesions are unknown. To probe the biological properties of A68 in vivo, and to assess interactions of A68 with endogenous proteins in the rodent brain, we injected A68, dephosphorylated A68 (DEP-A68), and normal adult human tau protein into the hippocampus and neocortex of rats. In marked contrast to DEP-A68 and tau, A68 resisted rapid proteolysis and induced codeposits of three rodent proteins--i.e., beta A4, ubiquitin, and alpha 1-antichymotrypsin (ACT)--that accumulate in AD NFTs and SPs together with A68. These findings suggest that A68 may interact with beta A4, ubiquitin, and ACT in neuronal perikarya as well as in the extracellular space after release of A68 from degenerating neurons. The model system described here will facilitate efforts to elucidate mechanisms leading to the convergence of A68, beta A4, ubiquitin, and ACT in hallmark lesions of AD. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:8393578

  12. Antithrombin activities in childhood malnutrition.

    PubMed Central

    Jiménez, R A; Jiménez, E; Ingram, G I; Mora, L A; Atmetlla, F; Carrillo, J M; Vargas, W

    1979-01-01

    Antithrombin activities in 30 severely malnourished children and 40 normal children were estimated in clotting tests by thrombin neutralisation as anti-Xa and by a heparin antithrombin assay; and by immunodiffusion as alpha 2-globulin and alpha 1-antitrypsin. The patients' mean alpha 2-globulin was severely depressed, and there were less marked depletions in mean values for thrombin neutralisation, anti-Xa, and in the heparin antithrombin assay (which showed the flat curve thought to reflect a thrombotic tendency). The alpha 1-antitrypsin values were normal. The findings support the concept of antithrombin as the summation of alpha 2-globulin and alpha 1-antitrypsin (with alpha 2-macroglobulin); and the low values may be related to the high incidence of thrombosis reported in childhood malnutrition, although it was not seen in these patients. PMID:118190

  13. Magnetic particles as affinity matrix for purification of antithrombin

    NASA Astrophysics Data System (ADS)

    Mercês, A. A. D.; Maciel, J. C.; Carvalho Júnior, L. B.

    2015-11-01

    Immobilization of biomolecules onto insoluble supports is an important tool for the fabrication of a diverse range of functional materials. It provides advantages: enhanced stability and easy separation. In this work two different magnetic composites were synthesized (MAG-PANI-HS and mDAC-HS) to human antithrombin purification. The magnetic particles (MAG) were obtained by co-precipitation method of iron salts II and III and subsequently coated with polyaniline (MAG-PANI particles). Dacron (polyethylene terephthalate) suffered a hydrazinolysis reaction to obtain a powder (Dacron hydrazide) which was subsequently magnetized (mDAC particles) also by co-precipitation method. Heparan sulfate (HS) was immobilized to MAG-PANI and mDAC retained respectively 35μg and 38.6μg per of support. The magnetic composite containing HS immobilized (MAG-PANI-HS and mDAC-HS) was incubated with human blood plasma (1mL) and then washed with NaCl gradients. Electrophoresis of proteins present in eluates showed bands of antithrombin (58kDa). A reduction in the antithrombin activity was detected in plasma that were incubated in the composites magnetic with HS immobilized, suggesting that the antithrombin was removed of the human blood plasma and then purified. Therefore, the above results suggest that both preparations: MAG-PANI-HS and mDAC-HS are able to affinity purify antithrombin, an important component of blood coagulation.

  14. A role for pericellular proteoglycan in the binding of thrombin or antithrombin III by the blood vessel endothelium? The effects of proteoglycan-degrading enzymes and glycosaminoglycan-binding proteins on 125I-thrombin binding by the rabbit thoracic aorta in vitro.

    PubMed

    Hatton, M W; Moar, S L

    1985-04-22

    Rabbit thoracic aorta segments were treated with either proteoglycan-degrading enzymes or with glycosaminoglycan-binding proteins to examine the nature of the endothelial and subendothelial binding sites of 125I-thrombin. Treatment (5-30 min) with enzymes (heparitinase, chondroitinases AC or ABC) caused a decrease in 125I-thrombin binding by the endothelium (30-70%) and by the subendothelial (intima-media) layer (20-50%); a low-specificity protease destroyed endothelial binding almost entirely and reduced binding to the subendothelium by approximately 60% over a similar period. Of the glycosaminoglycan-binding proteins, pretreatment of the aorta wall with protamine caused a 30% decrease in thrombin binding to the endothelium whereas lipoprotein lipase (present during 125I-thrombin uptake) decreased binding by up to 40%. Pretreatment with antithrombin III did not significantly affect binding of either 125I-thrombin or 125I-FPR-inactivated thrombin. In contrast to thrombin, 125I-antithrombin III was not readily uptaken by the aorta segments. These observations indicate that, whereas the minimal binding by 125I-antithrombin III probably does not involve endothelial proteoglycan, a strong case can be made for endothelial and subendothelial proteoglycan binding sites for thrombin. PMID:4024034

  15. Association between α1-antichymotrypsin signal peptide -15A/T polymorphism and the risk of Alzheimer's disease: a meta-analysis.

    PubMed

    Guan, Fulin; Gu, Jiaao; Hu, Fulan; Zhu, Yulan; Wang, Weizhi

    2012-06-01

    No consensus has been recently reached at the relationship between the α1-antichymotrypsin (ACT) signal peptide -15A/T polymorphism and Alzheimer’s disease (AD) risk. Thus, our study aimed to better assess this association by performing a meta-analysis, including 4,212 cases and 4,039 controls from 29 studies. Odds ratios (ORs) with the 95% confidence interval (CI) were used to assess the strength of relationship between ACT -15A/T polymorphism and AD risk. Overall, a borderline statistically significant association was detected under recessive model comparison in all subjects (AA vs. AT+TT: OR 1.12, 95% CI 1.01-1.25, P = 0.04). But in subgroup analysis by ethnicity, no significant association was found in Caucasians, Asians, or Africans. Moreover, after exclusion of one study which affect the heterogeneity, the ACT A allele and AA genotype were statistically associated with late-onset AD (LOAD) risk (AA vs. TT: OR 1.25, 95% CI 1.06-1.48, P = 0.007, A vs. T: OR 1.12, 95% CI 1.03-1.21, P = 0.008), especially in Caucasians. In conclusion, our study suggests that the common α1-antichymotrypsin signal peptide -15A/T polymorphism may not be a major risk factor for AD. However, the polymorphism is capable of increasing LOAD risk. PMID:22294107

  16. Interstitial deletion of chromosome 1q [del(1)(q24q25.3)] identified by fluorescence in situ hybridization and gene dosage analysis of apolipoprotein A-II, coagulation factor V, and antithrombin III

    SciTech Connect

    Takano, Takako; Yamanouchi, Yasuko; Mori, Yosuke

    1997-01-20

    We report on a 12-month-old Japanese boy with an interstitial deletion of the long-arm of chromosome 1 and meningomyelocele, hydrocephalus, anal atresia, atrial septal defect, left renal agenesis, bilateral cryptorchidism, talipes equinovarus, low birth weight, growth/developmental retardation, and many minor anomalies. By conventional GTG-banding, his karyotype was first interpreted as 46,XY,de1(1)(q23q24), but it was corrected as 46,XY.ish del(1)(q24q25.3) by fluorescence in situ hybridization using 11 known cosmid clones as probes. His serum levels of apolipoprotein A-II (gene symbol: APOA2, previously assigned to 1q21-q23) and coagulation factor V (F5, 1q21-q25) were normal, while serum concentration and activity of antithrombin III (AT3, 1q23-q25.1) was low. The results indicated that localization of APOA2 and F5 are proximal to the deleted region and AT3 is located within the deletion extent in the patient. 16 refs., 4 figs.

  17. Insulin-like growth factors (IGFs) stimulate the release of alpha 1-antichymotrypsin and soluble IGF-II/mannose 6-phosphate receptor from MCF7 breast cancer cells.

    PubMed

    Confort, C; Rochefort, H; Vignon, F

    1995-09-01

    The growth of hormone-responsive MCF7 human breast cancer cells is controlled by steroid hormones and growth factors. By metabolic labeling of cells grown in steroid- and growth factor-stripped serum conditions, we show that insulin-like growth factors (IGF-I and IGF-II) increase by approximately 5-fold the release of several proteins including cathepsin D, alpha 1-antichymotrypsin, and soluble forms of the multifunctional IGF-II/mannose 6-phosphate (M6P) receptor. Two soluble forms of IGF-II/M6P receptors were detected, one major (approximately 260 kilodaltons) and one minor (approximately 85 kilodaltons) that probably represents a proteolytic fragment of the larger soluble molecule. IGFs increased receptor release in a dose-dependent fashion with 50-60% of newly synthesized receptor released at 5-10 nM IGFs. The release of IGF-II/M6P receptors correlated with the levels of secreted cathepsin D in different human breast cancer cells or in rats stable transfectants that are constitutively expressing variable levels of human cathepsin D. IGFs had a stronger effect on IGF-II/M6P receptor release, whereas estradiol treatment preferentially enhanced the release of protease and antiprotease. We thus demonstrate that in human breast cancer cells, IGFs not only act as strong mitogens but also regulate release of alpha 1-antichymotrypsin, IGF-II/M6P-soluble receptor, and cathepsin D; three proteins that potentially regulate cell proliferation and/or invasion. PMID:7649082

  18. Antithrombin Attenuates Vascular Leakage via Inhibiting Neutrophil Activation in Acute Lung Injury

    PubMed Central

    Rehberg, Sebastian; Yamamoto, Yusuke; Sousse, Linda E.; Jonkam, Collette; Zhu, Yong; Traber, Lillian D.; Cox, Robert A.; Prough, Donald S.; Traber, Daniel L.; Enkhbaatar, Perenlei

    2014-01-01

    Objective To test the hypothesis that restoration of antithrombin plasma concentrations attenuates vascular leakage by inhibiting neutrophil activation through syndecan-4 receptor inhibition in an established ovine model of acute lung injury. Design Randomized controlled laboratory experiment. Setting University animal research facility. Subjects Eighteen chronically instrumented sheep. Interventions Following combined burn and smoke inhalation injury (40% of total body surface area, third-degree flame burn; 4 × 12 breaths of cold cotton smoke), chronically instrumented sheep were randomly assigned to receive an IV infusion of 6 IU/kg/hr recombinant human antithrombin III or normal saline (n = 6 each) during the 48-hour study period. In addition, six sham animals (not injured, continuous infusion of vehicle) were used to obtain reference values for histological and immunohistochemical analyses. Measurements and Main Results Compared to control animals, recombinant human antithrombin III reduced the number of neutrophils per hour in the pulmonary lymph (p < 0.01 at 24 and 48 hr), alveolar neutrophil infiltration (p = 0.04), and pulmonary myeloperoxidase activity (p = 0.026). Flow cytometric analysis revealed a significant reduction of syndecan-4-positive neutrophils (p = 0.002 vs control at 24 hr). Treatment with recombinant human antithrombin III resulted in a reduction of pulmonary nitrosative stress (p = 0.002), airway obstruction (bronchi: p = 0.001, bronchioli: p = 0.013), parenchymal edema (p = 0.044), and lung bloodless wet-to-dry-weight ratio (p = 0.015). Clinically, recombinant human antithrombin III attenuated the increased pulmonary transvascular fluid flux (12–48 hr: p ≤ 0.001 vs control each) and the deteriorated pulmonary gas exchange (12–48 hr: p < 0.05 vs control each) without increasing the risk of bleeding. Conclusions The present study provides evidence for the interaction between antithrombin and neutrophils in vivo, its pathophysiological

  19. Interaction of thrombin des-ETW with antithrombin III, the Kunitz inhibitors, thrombomodulin and protein C. Structural link between the autolysis loop and the Tyr-Pro-Pro-Trp insertion of thrombin.

    PubMed

    Le Bonniec, B F; Guinto, E R; Esmon, C T

    1992-09-25

    X-ray diffraction studies of human thrombin revealed that compared with trypsin, two insertions (B and C) potentially limit access to the active site groove. When amino acids Glu146, Thr147, and Trp148, adjacent to the C-insertion (autolysis loop), are deleted the resulting thrombin (des-ETW) has dramatically altered interaction with serine protease inhibitors. Whereas des-ETW resists antithrombin III inactivation with a rate constant (Kon) approximately 350-fold slower than for thrombin, des-ETW is remarkably sensitive to the Kunitz inhibitors, with inhibition constants (Ki) decreased from 2.6 microM to 34 nM for the soybean trypsin inhibitor and from 52 microM to 1.8 microM for the bovine pancreatic trypsin inhibitor. The affinity for hirudin (Ki = 5.6 pM) is weakened at least 30-fold compared with recombinant thrombin. The mutation affects the charge stabilizing system and the primary binding pocket of thrombin as depicted by a decrease in Kon for diisopropylfluorophosphate (9.5-fold) and for N alpha-p-tosyl-L-lysine-chloromethyl ketone (51-fold) and a 39-fold increase in the Ki for benzamidine. With peptidyl p-nitroanilide substrates, the des-ETW deletion results in changes in the Michaelis (Km) and/or catalytic (kcat) constants, worsened as much as 85-fold (Km) or 100-fold (kcat). The specific clotting activity of des-ETW is less than 5% that of thrombin and the kcat/Km for protein C activation in the absence of cofactor less than 2%. Thrombomodulin binds to des-ETW with a dissociation constant of approximately 2.5 nM and partially restores its ability to activate protein C since, in the presence of the cofactor, kcat/Km rises to 6.5% that of thrombin. This study suggests that the ETW motif of thrombin prevents (directly or indirectly) its interaction with the two Kunitz inhibitors and is not essential for the thrombomodulin-mediated enhancement of protein C activation. PMID:1326550

  20. Alpha 1-Antichymotrypsin, an Inflammatory Protein Overexpressed in the Brains of Patients with Alzheimer's Disease, Induces Tau Hyperphosphorylation through c-Jun N-Terminal Kinase Activation.

    PubMed

    Tyagi, Ethika; Fiorelli, Tina; Norden, Michelle; Padmanabhan, Jaya

    2013-01-01

    The association of inflammatory proteins with neuritic plaques in the brains of Alzheimer's disease (AD) patients has led to the hypothesis that inflammation plays a pivotal role in the development of pathology in AD. Earlier studies have shown that alpha 1-antichymotrypsin (ACT) enhances amyloid beta fibrillization and accelerated plaque formation in APP transgenic mice. Later studies from our laboratory have shown that purified ACT induces tau hyperphosphorylation and degeneration in neurons. In order to understand the mechanisms by which inflammatory proteins enhance tau hyperphosphorylation, we injected interleukin-1 β (IL-1 β ) intracerebroventricularly into mice expressing human ACT, human tau, or both transgenes. It was found that the hyperphosphorylation of tau in ACT and ACT/htau mice after IL-1 β injection correlated with increased phosphorylation of c-Jun N-terminal kinase (JNK). We verified the involvement of JNK in ACT-induced tau phosphorylation by utilizing JNK inhibitors in cultured primary neurons treated with ACT, and we found that the inhibitor showed complete prevention of ACT-induced tau phosphorylation. These results indicate that JNK is one of the major kinases involved in the ACT-mediated tau hyperphosphorylation and suggest that inhibitors of this kinase may protect against inflammation-induced tau hyperphosphorylation and neurodegeneration associated with AD. PMID:24175110

  1. Plasma levels of alpha1-antichymotrypsin and secretory leukocyte proteinase inhibitor in healthy and chronic obstructive pulmonary disease (COPD) subjects with and without severe α1-antitrypsin deficiency

    PubMed Central

    Hollander, Camilla; Westin, Ulla; Wallmark, Anders; Piitulainen, Eeva; Sveger, Tomas; Janciauskiene, Sabina M

    2007-01-01

    Background Individuals with severe Z α1-antitrypsin (AAT) deficiency have a considerably increased risk of developing chronic obstructive lung disease (COPD). It has been hypothesized that compensatory increases in levels of other protease inhibitors mitigate the effects of this AAT deficiency. We analysed plasma levels of AAT, α1-antichymotrypsin (ACT) and secretory leukocyte protease inhibitor (SLPI) in healthy (asymptomatic) and COPD subjects with and without AAT deficiency. Methods Studied groups included: 71 asymptomatic AAT-deficient subjects (ZZ, n = 48 and SZ, n = 23, age 31 ± 0.5) identified during Swedish neonatal screening for AAT deficiency between 1972 and 1974; age-matched controls (MM, n = 57, age 30.7 ± 0.6); older asymptomatic ZZ (n = 10); healthy MM (n = 20, age 53 ± 9.6); and COPD patients (ZZ, n = 10, age 47.4 ± 11 and MM, n = 10, age 59.4 ± 6.7). Plasma levels of SLPI, AAT and ACT were analysed using ELISA and immunoelectrophoresis. Results No significant difference was found in plasma ACT and SLPI levels between the healthy MM and the ZZ or SZ subjects in the studied groups. Independent of the genetic variant, subjects with COPD (n = 19) had elevated plasma levels of SLPI and ACT relative to controls (n = 153) (49.5 ± 7.2 vs 40.7 ± 9.1 ng/ml, p < 0.001 and 0.52 ± 0.19 vs 0.40 ± 0.1 mg/ml, p < 0.05, respectively). Conclusion Our findings show that plasma levels of ACT and SLPI are not elevated in subjects with genetic AAT deficiency compared MM controls and do not appear to compensate for the deficiency of plasma AAT. PMID:17261175

  2. Is Alpha-1 Antichymotrypsin Gene Polymorphism a Risk Factor for Primary Intracerebral Hemorrhage? A Case-Control Study and Meta-Analysis

    PubMed Central

    Hu, Xin; Li, Yunke; Li, Hao; Fang, Yuan; Liu, Ming; You, Chao

    2015-01-01

    Background Alpha-1 antichymotrypsin (ACT) signal peptide A/T polymorphism has been suggested to play a role in various brain diseases with arterial wall pathology. We conducted a case-control study and a meta-analysis to evaluate the association between this polymorphism and risk of primary intracerebral hemorrhage. Material/Methods A total of 188 patients and 200 age- and sex-matched healthy controls were enrolled in our case-control study. The ACT polymorphism was genotyped by PCR-LDR. Further meta-analysis was conducted by searching literature from PUBMED, EMBASE, and Chinese National Knowledge Infrastructure databases until December 2014, then combining data using STATA10.0. Results Similar genotype distribution was detected between PICH patients and healthy controls (p=0.523). Further analysis based on hypertension and location of hemorrhage did not observe significant association. Multiple logistic regression analysis also failed to identify ACT polymorphism as an independent risk factor for PICH. With regard to meta-analysis, a total of 6 case-control studies including 932 PICH patients and 1140 controls were enrolled. Pooled ORs failed to detect a significant association of ACT signal peptide A/T polymorphism with PICH (dominant model: OR=1.03, 95%CI=0.72–1.46; recessive model: OR=1.08, 95%CI=0.88–1.32). Subgroup analysis based on hypertension revealed no association in hypertensive PICH or in normotensive PICH. Conclusions Our case-control study in a Chinese population did not detect a significant association between ACT signal peptide A/T polymorphism and PICH. Moreover, meta-analysis combining data from relevant studies failed to provide evidence for the association. Further well-designed studies with larger sample sizes are warranted to verify our findings. PMID:26210716

  3. Antithrombin, an Important Inhibitor in Blood Clots.

    PubMed

    Zhu, Ying; Cong, Qing-Wei; Liu, Yue; Wan, Chun-Ling; Yu, Tao; He, Guang; He, Lin; Cai, Lei; Chou, Kuo-Chen

    2016-01-01

    Blood coagulation is healthy and lifesaving because it can stop bleeding. It can, however, be a troublemaker as well, causing serious medical problems including heart attack and stroke. Body has complex blood coagulation cascade to modulate the blood clots. In the environment of plasma, the blood coagulation cascade is regulated by antithrombin, which is deemed one of the most important serine protease inhibitors. It inhibits thrombin; it can inhibit factors IXa and Xa as well. Interestingly, its inhibitory ability will be significantly increased with the existence of heparin. In this minireview paper, we are to summarize the structural features of antithrombin, as well as its heparin binding modes and anti-coagulation mechanisms, in hopes that the discussion and analysis presented in this paper can stimulate new strategies to find more effective approaches or compounds to modulate the antithrombin. PMID:26411319

  4. Neuraxial anesthesia for labor and cesarean delivery in a parturient with hereditary antithrombin deficiency on recombinant human antithrombin infusion therapy.

    PubMed

    Pamnani, Anup; Rosenstein, Megan; Darwich, Alaeldin; Wolfson, Alexander

    2010-09-01

    A recombinant human antithrombin (rhAT; generic name: antithrombin Alfa) has recently been developed. A 37 year-old parturient with hereditary antithrombin deficiency, receiving rhAT infusion therapy, who successfully received an epidural catheter for analgesia and anesthesia during labor and cesarean delivery, is presented. PMID:20868967

  5. Solid serous cystadenoma of the pancreas: a case report of 2 patients revealing vimentin, β-catenin, α-1 antitrypsin, and α-1 antichymotrypsin as new immunohistochemistry staining markers.

    PubMed

    Wu, Wenming; Hong, Xiafei; Li, Ji; Dai, Menghua; Wang, Wenze; Tong, Anli; Zhu, Zhaohui; Dai, Hongmei; Zhao, Yupei

    2015-03-01

    Solid serous cystadenoma (SCA) of the pancreas is a rare type of pancreatic solid tumors. Postoperative pathological evaluation is of particular importance for distinguishing solid SCA of the pancreas from other pancreatic solid tumors. Here we present 2 cases of solid SCA of the pancreas, both preoperatively diagnosed with pancreatic neuroendocrine tumors. One case had positive OctreoScan test. Surgical resections were done for both cases. Postoperative immunohistochemistry assays were conducted with marker panels for SCA and 2 types of pancreatic solid tumors, which were neuroendocrine tumor (pNET) and solid pseudopapillary tumor (SPT). Two cases showed typical staining patterns for SCA markers. Notably, both cases showed positivity for 4 SPT markers (vimentin, β-catenin, α-1 antitrypsin, and α-1 antichymotrypsin). Emphasis should be paid to those 4 new markers for future pathological diagnosis of solid SCA of the pancreas. PMID:25816032

  6. Solid Serous Cystadenoma of the Pancreas: A Case Report of 2 Patients Revealing Vimentin, β-Catenin, α-1 Antitrypsin, and α-1 Antichymotrypsin as New Immunohistochemistry Staining Markers

    PubMed Central

    Wu, Wenming; Hong, Xiafei; Li, Ji; Dai, Menghua; Wang, Wenze; Tong, Anli; Zhu, Zhaohui; Dai, Hongmei; Zhao, Yupei

    2015-01-01

    Abstract Solid serous cystadenoma (SCA) of the pancreas is a rare type of pancreatic solid tumors. Postoperative pathological evaluation is of particular importance for distinguishing solid SCA of the pancreas from other pancreatic solid tumors. Here we present 2 cases of solid SCA of the pancreas, both preoperatively diagnosed with pancreatic neuroendocrine tumors. One case had positive OctreoScan test. Surgical resections were done for both cases. Postoperative immunohistochemistry assays were conducted with marker panels for SCA and 2 types of pancreatic solid tumors, which were neuroendocrine tumor (pNET) and solid pseudopapillary tumor (SPT). Two cases showed typical staining patterns for SCA markers. Notably, both cases showed positivity for 4 SPT markers (vimentin, β-catenin, α-1 antitrypsin, and α-1 antichymotrypsin). Emphasis should be paid to those 4 new markers for future pathological diagnosis of solid SCA of the pancreas. PMID:25816032

  7. Characterization of Thrombate III®, a pasteurized and nanofiltered therapeutic human antithrombin concentrate.

    PubMed

    Cai, Kang; Osheroff, Wendy P; Buczynski, Greg; Hotta, JoAnn; Lang, John; Elliott, Eric; Lee, Douglas C; Roth, Nathan J

    2014-05-01

    Thrombate III(®) is a highly purified antithrombin concentrate that has been used by clinicians worldwide for more than two decades for the treatment of hereditary antithrombin deficiency. The manufacturing process is based on heparin-affinity chromatography and pasteurization. To modernize the process and to further enhance the pathogen safety profile of the final product, despite the absence of infectious disease transmission, a nanofiltration step was added. The biochemical characterization and pathogen safety evaluation of Thrombate III(®) manufactured using the modernized process are presented. Bioanalytical data demonstrate that the incorporation of nanofiltration has no impact on the antithrombin content, potency, and purity of the product. Scaledown models of the manufacturing process were used to assess virus and prion clearance under manufacturing setpoint conditions. Additionally, robustness of virus clearance was evaluated at or slightly outside the manufacturing operating limits. The results demonstrate that pasteurization inactivated both enveloped and non-enveloped viruses. The addition of nanofiltration substantially increased clearance capacities for both enveloped and non-enveloped viruses by approximately 4-6 log10. In addition, the process achieves 6.0 log10 ID50 prion infectivity clearance. Thus, the introduction of nanofiltration increased the pathogen safety margin of the manufacturing process without impacting the key biochemical characteristics of the product. PMID:24477183

  8. Physical mapping of four serpin genes: [alpha][sub 1]-antitrypsin, [alpha][sub 1]-antichymotrypsin, corticosteroid-binding globulin, and protein C inhibitor, within a 280-kb region on chromosome 14q32. 1

    SciTech Connect

    Billingsley, G.D.; Cox, D.W. Univ. of Toronto, Ontario ); Walter, M.A. ); Hammond, G.L. )

    1993-02-01

    Alpha[sub 1]-antitrypsin ([alpha]1AT; protease inhibitor [PI] locus), [alpha][sub 1]-antichymotrypsin ([alpha]1ACT; AACT locus), corticosteroid-binding globulin (CBG; CBG locus), and protein C inhibitor (PCI; PCI locus) are members of the serine protease inhibitor (serpin) superfamily. A noncoding PI-like (PIL) gene has been located 12 kb 3[prime] of the PI gene. The PI, PIL, and AACT loci have been localized to 14q32.1, the CBG locus has been localized to 14q31-14q32.1, and PCI has been mapped to chromosome 14. Genetic linkage analysis suggests tight linkage between PI and AACT. The authors have used pulsed-field gel electrophoresis to generate a physical map linking these five serpin genes. The order of the genetic loci is AACT/PCI-PI-PIL-CBG, with a maximum distance of about 220 kb between the AACT/PCI and PI genes. These genes form a PI cluster at 14q32.1, similar to that of the homologous genes on murine chromosome 12. The close proximity of these genes has implications for disease-association studies. 44 refs., 6 figs., 2 tabs.

  9. Antithrombin Dublin (p.Val30Glu): a relatively common variant with moderate thrombosis risk of causing transient antithrombin deficiency.

    PubMed

    Navarro-Fernández, José; de la Morena-Barrio, María Eugenia; Padilla, José; Miñano, Antonia; Bohdan, Nataliya; Águila, Sonia; Martínez-Martínez, Irene; Sevivas, Teresa S; de Cos, Carmen; Fernández-Mosteirín, Nuria; Llamas, Pilar; Asenjo, Susana; Medina, Pilar; Souto, Juan Carlos; Overvad, Kim; Kristensen, Søren R; Corral, Javier; Vicente, Vicente

    2016-07-01

    The key haemostatic role of antithrombin and the risk of thrombosis associated with its deficiency support that the low incidence of antithrombin deficiency among patients with thrombosis might be explained by underestimation of this disorder. It was our aim to identify mutations in SERPINC1 causing transient antithrombin deficiency. SERPINC1 was sequenced in 214 cases with a positive test for antithrombin deficiency, including 67 with no deficiency in the sample delivered to our laboratory. The p.Val30Glu mutation (Antithrombin Dublin) was identified in five out of these 67 cases, as well as in three out of 127 cases with other SERPINC1 mutations. Genotyping in 1593 patients with venous thrombosis and 2592 controls from two populations, revealed a low prevalent polymorphism (0.3 %) that moderately increased the risk of venous thrombosis (OR: 2.9; 95 % CI: 1.07-8.09; p= 0.03) and identified one homozygous patient with an early thrombotic event. Carriers had normal anti-FXa activity, and plasma antithrombin was not sensitive to heat stress or proteolytic cleavage. Analysis of one sample with transient deficit revealed a type I deficiency, without aberrant or increased latent forms. The recombinant variant, which lacked the two amino-terminal residues, had reduced secretion from HEK-EBNA cells, formed hyperstable disulphide-linked polymers, and had negligible activity. In conclusion, p.Val30Glu by affecting the cleavage of antithrombin's signal peptide, results in a mature protein lacking the N-terminal dipeptide with no functional consequences in normal conditions, but that increases the sensitivity to be folded intracellularly into polymers, facilitating transient antithrombin deficiency and the subsequent risk of thrombosis. PMID:27098529

  10. Antithrombin in the treatment of burn trauma.

    PubMed

    Kowal-Vern, Areta; Orkin, Bruce A

    2016-02-01

    Antithrombin (AT) is a natural anticoagulant with anti-inflammatory properties that has demonstrated value in sepsis, disseminated intravascular coagulation and in burn and inhalation injury. With high doses, AT may decrease blood loss during eschar excision, reducing blood transfusion requirements. There are no human randomized, placebo-controlled studies, which have tested the true benefit of this agent in these conditions. Two main forms of AT are either plasma-derived AT (phAT) and recombinant AT (rhAT). Major ovine studies in burn and smoke inhalation injury have utilized rhAT. There have been no studies which have either translated the basic rhAT research in burn trauma, or determined the tolerance and pharmacokinetics of rhAT concentrate infusions in burn patients. Advantages of rhAT infusions are no risk of blood borne diseases and lower cost. However, the majority of human burn patient studies have been conducted utilizing phAT. Recent Japanese clinical trials have started using phAT in abdominal sepsis successfully. This review examines the properties of both phAT and rhAT, and analyzes studies in which they have been utilized. We believe that it is time to embark on a randomized placebo-controlled multi-center trial to establish the role of AT in both civilian and military patients with burn trauma. PMID:26855890

  11. Antithrombin in the treatment of burn trauma

    PubMed Central

    Kowal-Vern, Areta; Orkin, Bruce A

    2016-01-01

    Antithrombin (AT) is a natural anticoagulant with anti-inflammatory properties that has demonstrated value in sepsis, disseminated intravascular coagulation and in burn and inhalation injury. With high doses, AT may decrease blood loss during eschar excision, reducing blood transfusion requirements. There are no human randomized, placebo-controlled studies, which have tested the true benefit of this agent in these conditions. Two main forms of AT are either plasma-derived AT (phAT) and recombinant AT (rhAT). Major ovine studies in burn and smoke inhalation injury have utilized rhAT. There have been no studies which have either translated the basic rhAT research in burn trauma, or determined the tolerance and pharmacokinetics of rhAT concentrate infusions in burn patients. Advantages of rhAT infusions are no risk of blood borne diseases and lower cost. However, the majority of human burn patient studies have been conducted utilizing phAT. Recent Japanese clinical trials have started using phAT in abdominal sepsis successfully. This review examines the properties of both phAT and rhAT, and analyzes studies in which they have been utilized. We believe that it is time to embark on a randomized placebo-controlled multi-center trial to establish the role of AT in both civilian and military patients with burn trauma. PMID:26855890

  12. Genetics Home Reference: hereditary antithrombin deficiency

    MedlinePlus

    ... legs. This type of clot is called a deep vein thrombosis (DVT). Affected individuals also have an increased risk of ... III Deficiency Health Topic: Blood Clots Health Topic: Deep Vein Thrombosis Health Topic: Pulmonary Embolism Genetic and Rare Diseases ...

  13. Recombinant Human Antithrombin in Pregnant Patients with Hereditary Antithrombin Deficiency: Integrated Analysis of Clinical Data.

    PubMed

    Paidas, Michael J; Triche, Elizabeth W; James, Andra H; DeSancho, Maria; Robinson, Christopher; Lazarchick, John; Ornaghi, Sara; Frieling, Johan

    2016-03-01

    Objectives The purpose of this analysis was to evaluate the use of recombinant human antithrombin (rhAT) in preventing venous thromboembolism (VTE) in pregnant patients with hereditary AT deficiency (HATD). Study Design Data from two clinical trials were pooled. Dosing of rhAT was based on body weight and baseline AT activity, started up to 24 hours before scheduled induction or cesarean delivery, or at the onset of labor. Results A total of 21 pregnant HATD patients were enrolled. Mean rhAT therapy duration was 4.3 days and dose was 245.1 IU/kg/day. All patients achieved target mean AT activity (80-120% of normal) during rhAT therapy. There were no confirmed VTEs during rhAT treatment or within 7 ( ± 1) days after dosing. Two VTE events (one deep vein thrombosis and one pulmonary embolism) occurred 11 and 14 days after discontinuation of rhAT, in patients managed with prophylactic doses of heparin or low-molecular-weight heparin following delivery. Conclusion rhAT was safe and effective in pregnant HATD patients when administered during the peripartum period, the period of highest VTE risk and a time when anticoagulation therapy is normally withheld. Pregnant HATD patients may benefit from therapeutic, rather than prophylactic, doses of anticoagulation after delivery to protect against postpartum VTE. PMID:26461927

  14. Inherited antithrombin deficiency and anabolic steroids: a risky combination.

    PubMed

    Choe, Hannah; Elfil, Mohamed; DeSancho, Maria T

    2016-09-01

    A 20-year-old male with asymptomatic inherited type 1 antithrombin deficiency and a family history of thrombosis started injecting himself with testosterone 250 mg intramuscularly twice weekly for 5 weeks. He presented to the hospital with progressive dyspnea on exertion, chest pain and hemoptysis. Workup revealed bilateral submassive pulmonary embolism and proximal right lower extremity deep vein thrombosis. He was treated with intravenous (IV) unfractionated heparin and underwent catheter-directed thrombolysis with alteplase to the main pulmonary arteries. Postprocedure, he remained on IV alteplase infusion for 24 h and unfractionated heparin in the intensive care unit. Concomitantly he received plasma-derived antithrombin concentrate. He was transitioned to subcutaneous enoxaparin twice daily and discharged from the hospital on oral rivaroxaban 15 mg twice a day. This case highlights the heightened thrombogenic effect of anabolic steroids in the setting of underlying thrombophilia especially in younger subjects. PMID:26588446

  15. Antithrombin controls tumor migration, invasion and angiogenesis by inhibition of enteropeptidase

    PubMed Central

    Luengo-Gil, Ginés; Calvo, María Inmaculada; Martín-Villar, Ester; Águila, Sonia; Bohdan, Nataliya; Antón, Ana I.; Espín, Salvador; Ayala de la Peña, Francisco; Vicente, Vicente; Corral, Javier; Quintanilla, Miguel; Martínez-Martínez, Irene

    2016-01-01

    Antithrombin is a key inhibitor of the coagulation cascade, but it may also function as an anti-inflammatory, anti-angiogenic, anti-viral and anti-apoptotic protein. Here, we report a novel function of antithrombin as a modulator of tumor cell migration and invasion. Antithrombin inhibited enteropeptidase on the membrane surface of HT-29, A549 and U-87 MG cells. The inhibitory process required the activation of antithrombin by heparin, and the reactive center loop and the heparin binding domain were essential. Surprisingly, antithrombin non-covalently inhibited enteropeptidase, revealing a novel mechanism of inhibition for this serpin. Moreover, as a consequence of this inhibition, antithrombin was cleaved, resulting in a molecule with anti-angiogenic properties that reduced vessel-like formation of endothelial cells. The addition of antithrombin and heparin to U-87 MG and A549 cells reduced motility in wound healing assays, inhibited the invasion in transwell assays and the degradation of a gelatin matrix mediated by invadopodia. These processes were controlled by enteropeptidase, as demonstrated by RNA interference experiments. Carcinoma cell xenografts in nude mice showed in vivo co-localization of enteropeptidase and antithrombin. Finally, treatment with heparin reduced experimental metastasis induced by HT29 cells in vivo. In conclusion, the inhibition of enteropeptidase by antithrombin may have a double anti-tumor effect through inhibiting a protease involved in metastasis and generating an anti-angiogenic molecule. PMID:27270881

  16. Antithrombin controls tumor migration, invasion and angiogenesis by inhibition of enteropeptidase.

    PubMed

    Luengo-Gil, Ginés; Calvo, María Inmaculada; Martín-Villar, Ester; Águila, Sonia; Bohdan, Nataliya; Antón, Ana I; Espín, Salvador; Ayala de la Peña, Francisco; Vicente, Vicente; Corral, Javier; Quintanilla, Miguel; Martínez-Martínez, Irene

    2016-01-01

    Antithrombin is a key inhibitor of the coagulation cascade, but it may also function as an anti-inflammatory, anti-angiogenic, anti-viral and anti-apoptotic protein. Here, we report a novel function of antithrombin as a modulator of tumor cell migration and invasion. Antithrombin inhibited enteropeptidase on the membrane surface of HT-29, A549 and U-87 MG cells. The inhibitory process required the activation of antithrombin by heparin, and the reactive center loop and the heparin binding domain were essential. Surprisingly, antithrombin non-covalently inhibited enteropeptidase, revealing a novel mechanism of inhibition for this serpin. Moreover, as a consequence of this inhibition, antithrombin was cleaved, resulting in a molecule with anti-angiogenic properties that reduced vessel-like formation of endothelial cells. The addition of antithrombin and heparin to U-87 MG and A549 cells reduced motility in wound healing assays, inhibited the invasion in transwell assays and the degradation of a gelatin matrix mediated by invadopodia. These processes were controlled by enteropeptidase, as demonstrated by RNA interference experiments. Carcinoma cell xenografts in nude mice showed in vivo co-localization of enteropeptidase and antithrombin. Finally, treatment with heparin reduced experimental metastasis induced by HT29 cells in vivo. In conclusion, the inhibition of enteropeptidase by antithrombin may have a double anti-tumor effect through inhibiting a protease involved in metastasis and generating an anti-angiogenic molecule. PMID:27270881

  17. Identification of Regulatory Mutations in SERPINC1 Affecting Vitamin D Response Elements Associated with Antithrombin Deficiency

    PubMed Central

    Toderici, Mara; de la Morena-Barrio, María Eugenia; Padilla, José; Miñano, Antonia; Antón, Ana Isabel; Iniesta, Juan Antonio; Herranz, María Teresa; Fernández, Nuria; Vicente, Vicente; Corral, Javier

    2016-01-01

    Antithrombin is a crucial anticoagulant serpin whose even moderate deficiency significantly increases the risk of thrombosis. Most cases with antithrombin deficiency carried genetic defects affecting exons or flanking regions of SERPINC1.We aimed to identify regulatory mutations inSERPINC1 through sequencing the promoter, intron 1 and 2 of this gene in 23 patients with antithrombin deficiency but without known genetic defects. Three cases with moderate antithrombin deficiency (63–78%) carried potential regulatory mutations. One located 200 bp before the initiation ATG and two in intron 1. These mutations disrupted two out of five potential vitamin D receptor elements (VDRE) identified in SERPINC1 with different software. One genetic defect, c.42-1060_-1057dupTTGA, was a new low prevalent polymorphism (MAF: 0.01) with functional consequences on plasma antithrombin levels. The relevance of the vitamin D pathway on the regulation of SERPINC1 was confirmed in a cell model. Incubation of HepG2 with paricalcitol, a vitamin D analog, increased dose-dependently the levels of SERPINC1transcripts and antithrombin released to the conditioned medium. This study shows further evidence of the transcriptional regulation of SERPINC1 by vitamin D and first describes the functional and pathological relevance of mutations affecting VDRE of this gene. Our study opens new perspectives in the search of new genetic defects involved in antithrombin deficiency and the risk of thrombosis as well as in the design of new antithrombotic treatments. PMID:27003919

  18. Heparanase Activates Antithrombin through the Binding to Its Heparin Binding Site

    PubMed Central

    Águila, Sonia; Teruel-Montoya, Raúl; Vicente, Vicente; Corral, Javier; Martínez-Martínez, Irene

    2016-01-01

    Heparanase is an endoglycosidase that participates in morphogenesis, tissue repair, heparan sulphates turnover and immune response processes. It is over-expressed in tumor cells favoring the metastasis as it penetrates the endothelial layer that lines blood vessels and facilitates the metastasis by degradation of heparan sulphate proteoglycans of the extracellular matrix. Heparanase may also affect the hemostatic system in a non-enzymatic manner, up-regulating the expression of tissue factor, which is the initiator of blood coagulation, and dissociating tissue factor pathway inhibitor on the cell surface membrane of endothelial and tumor cells, thus resulting in a procoagulant state. Trying to check the effect of heparanase on heparin, a highly sulphated glycosaminoglycan, when it activates antithrombin, our results demonstrated that heparanase, but not proheparanase, interacted directly with antithrombin in a non-covalent manner. This interaction resulted in the activation of antithrombin, which is the most important endogenous anticoagulant. This activation mainly accelerated FXa inhibition, supporting an allosteric activation effect. Heparanase bound to the heparin binding site of antithrombin as the activation of Pro41Leu, Arg47Cys, Lys114Ala and Lys125Alaantithrombin mutants was impaired when it was compared to wild type antithrombin. Intrinsic fluorescence analysis showed that heparanase induced an activating conformational change in antithrombin similar to that induced by heparin and with a KD of 18.81 pM. In conclusion, under physiological pH and low levels of tissue factor, heparanase may exert a non-enzymatic function interacting and activating the inhibitory function of antithrombin. PMID:27322195

  19. Small Molecule Probes That Perturb A Protein-protein Interface In Antithrombin.

    PubMed

    Xin, Dongyue; Holzenburg, Andreas; Burgess, Kevin

    2014-12-01

    Small molecule probes for perturbing protein-protein interactions (PPIs) in vitro can be useful if they cause the target proteins to undergo biomedically relevant changes to their tertiary and quaternary structures. Application of the Exploring Key Orientations (EKO) strategy (J. Am. Chem. Soc., 2013, 135, 167 - 173) to a piperidinone-piperidine chemotype 1 indicated specific derivatives were candidates to perturb a protein-protein interface in the α-antithrombin dimer; those particular derivatives of 1 were prepared and tested. In the event, most of them significantly accelerated oligomerization of monomeric α-antithrombin, which is metastable in its oligomeric state. This assertion is supported by data from gel electrophoresis (non-denaturing PAGE; throughout) and probe-induced loss of α-antithrombin's inhibitor activity in a reaction catalyzed by thrombin. Kinetics of α-antithrombin oligomerization induced by the target compounds were examined. It was found that probes with O-benzyl-protected serine side-chains are the most active catalysts in the series, and reasons for this, based on modeling experiments, are proposed. Overall, this study reveals one of the first examples of small molecules designed to act at a protein-protein interface relevant to oligomerization of a serpin (ie α-antithrombin). The relevance of this to formation of oligomeric serpin fibrils, associated with the disease states known as "serpinopathies", is discussed. PMID:25396040

  20. Antithrombin is protective against myocardial ischemia and reperfusion injury

    PubMed Central

    Wang, Jingying; Wang, Yanqing; Wang, Jinli; Gao, Junjie; Tong, Chao; Manithody, Chandrashekhara; Li, Ji; Rezaie, Alireza R.

    2013-01-01

    Summary Background Antithrombin (AT) is a plasma serpin inhibitor that regulates the proteolytic activity of procoagulant proteases of the clotting cascade. In addition to its anticoagulant activity, AT also possesses potent antiinflammatory properties. Objectives The objective of this study is to investigate the antiinflammatory activity of wild-type AT (AT-WT) and a reactive center loop mutant of AT (AT-RCL) which is not capable of inhibiting thrombin. Methods Cardioprotective activities of AT-WT and AT-RCL were monitored in a mouse model of ischemia/reperfusion injury in which the left anterior descending coronary artery was occluded and then released. Results We demonstrate that AT markedly reduces myocardial infarct size by a mechanism that is independent of its anticoagulant activity. Thus, AT-RCL attenuated myocardial infarct size to the same extent as AT-WT in this acute injury model. Further studies revealed that AT binds to vascular heparan sulfate proteoglycans via its heparin-binding domain to exert its protective activity as evidenced by the therapeutic AT-binding pentasaccharide (fondaparinux) abrogating the cardioprotective activity of AT and a heparin-site mutant of AT exhibiting no cardioprotective property. We further demonstrate that AT up-regulates production of prostacyclin in myocardial tissues and inhibits expression of proinflammatory cytokines TNF-α and IL-6 in vivo by attenuating ischemia/reperfusion-induced JNK and NF-κB signaling pathways. Conclusions Our results suggest that both AT and the non-anticoagulant AT-RCL, through their antiinflammatory signaling effects, elicit potent cardioprotective responses. Thus, AT may have therapeutic potential for treating cardiac ischemia/reperfusion injury. PMID:23582062

  1. Factor VIIa-antithrombin complexes in patients with arterial and venous thrombosis.

    PubMed

    Spiezia, Luca; Rossetto, Valeria; Campello, Elena; Gavasso, Sabrina; Woodhams, Barry; Tormene, Daniela; Simioni, Paolo

    2010-06-01

    Antithrombin (AT), in the presence of heparin, is able to inhibit the catalytic activity of factor VIIa bound to tissue factor (TF) on cell surfaces. The clinical meaning of FVIIa-AT complexes plasma levels is unknown. It was the objective of this study to evaluate FVIIa-AT complexes in subjects with thrombosis. Factor VIIa-AT complexes plasma levels in 154 patients consecutively referred to our Department with arterial or venous thrombosis and in a group of 154 healthy subjects, were measured. Moreover, FVIIa-AT complexes were determined in: i) n = 53 subjects belonging to 10 families with inherited factor VII deficiency; ii) n = 58 subjects belonging to seven families with AT deficiency; iii) n = 49 patients undergoing oral anticoagulant therapy (OAT). Factor VIIa-AT levels were determined by a specific ELISA kit (R&D, Diagnostica Stago, Gennevilliers, France). Factor VIIa-AT complexes mean plasma levels were lower in patients with either acute arterial (136 +/- 40 pM) or venous (142 +/- 53 pM) thrombosis than subjects with previous thrombosis (arterial 164 +/- 33 pM and venous 172 +/- 61 pM, respectively) and than healthy controls (156 +/- 63 pM). Differences between acute and previous thrombosis, were statistically significant (p < 0.05). Subjects with inherited and acquired (under OAT) factor VII deficiency had statistically significant lower FVIIa-AT complexes plasma levels (80 +/- 23 pM and 55 +/- 22 pM, respectively) than controls (150 +/- 51 pM, p < 0.0001 and 156 +/- 63 pM, p < 0.00001, respectively). Factor VIIa-AT complexes are positively correlated with plasma factor VII/VIIa levels. Further investigations are needed to verify the possible role of higher FVIIa-AT complex plasma levels in predicting hypercoagulable states and thrombosis. PMID:20431847

  2. Small Molecule Probes That Perturb A Protein-protein Interface In Antithrombin

    PubMed Central

    Xin, Dongyue; Holzenburg, Andreas

    2014-01-01

    Small molecule probes for perturbing protein-protein interactions (PPIs) in vitro can be useful if they cause the target proteins to undergo biomedically relevant changes to their tertiary and quaternary structures. Application of the Exploring Key Orientations (EKO) strategy (J. Am. Chem. Soc., 2013, 135, 167 – 173) to a piperidinone-piperidine chemotype 1 indicated specific derivatives were candidates to perturb a protein-protein interface in the α-antithrombin dimer; those particular derivatives of 1 were prepared and tested. In the event, most of them significantly accelerated oligomerization of monomeric α-antithrombin, which is metastable in its oligomeric state. This assertion is supported by data from gel electrophoresis (non-denaturing PAGE; throughout) and probe-induced loss of α-antithrombin’s inhibitor activity in a reaction catalyzed by thrombin. Kinetics of α-antithrombin oligomerization induced by the target compounds were examined. It was found that probes with O-benzyl-protected serine side-chains are the most active catalysts in the series, and reasons for this, based on modeling experiments, are proposed. Overall, this study reveals one of the first examples of small molecules designed to act at a protein-protein interface relevant to oligomerization of a serpin (ie α-antithrombin). The relevance of this to formation of oligomeric serpin fibrils, associated with the disease states known as “serpinopathies”, is discussed. PMID:25396040

  3. Acceleration of thrombin-antithrombin complex formation in rat hindquarters via heparinlike molecules bound to the endothelium.

    PubMed Central

    Marcum, J A; McKenney, J B; Rosenberg, R D

    1984-01-01

    We have examined the role of heparinlike molecules in the regulation of coagulation by perfusing rat hindquarters with purified human thrombin and with its plasma inhibitor, antithrombin. Our data indicate that contact of the hemostatic components with the endothelium enhances the rate of thrombin-antithrombin complex formation by as much as 19-fold over the uncatalyzed rate of enzyme-inhibitor interaction. Heparinlike molecules are responsible for the antithrombin accelerating activity. The amount of thrombin-antithrombin complex generated within the hindlimb preparation after pretreatment of the vasculature with purified Flavobacterium heparinase or with addition of platelet Factor IV to the hemostatic components, was equal to the uncatalyzed levels. These heparinlike molecules appear to be tightly bound to the luminal surface of the endothelium, since they could not be detected within the physiologic buffer that was perfused through the animal. The above mucopolysaccharides function in a manner similar to commercial heparin, since modification of antithrombin at a site critical for heparin-dependent acceleration of the protease inhibitor resulted in a level of interaction product identical to the uncatalyzed amount. Finally, addition of diisofluorophosphate-thrombin to the enzyme perfusion stream reduced the amount of thrombin-antithrombin complex formed in the animal by 30-40%, which suggested that thrombin bound to the endothelium as well as enzyme free in solution are accessible to antithrombin that has interacted with heparinlike molecules present on the endothelium. PMID:6746897

  4. Regions flanking exon 1 regulate constitutive expression of the human antithrombin gene.

    PubMed

    Fernandez-Rachubinski, F A; Weiner, J H; Blajchman, M A

    1996-11-15

    We have identified cis-acting elements and trans-acting factors that regulate constitutive expression of the human antithrombin gene. The activity of the sequences flanking the first exon of the gene was investigated using a luciferase-based reporter assay in transiently transfected HepG2, COS1, BSC40, and HeLa cells. Deletion analysis allowed the mapping of two elements able to promote antithrombin gene transcription in HepG2 and COS1 cells. The first element is located upstream of the first exon (-150/+68 nucleotides). The second element is in the first intervening sequence (+300/+700 nucleotides) and functions in an orientation opposite to that of the first. Footprint analysis showed three protected areas in the 5' upstream element at -92/-68 (element A), -14/+37 (element B), and -126/-100 nucleotides (element C). These elements acted as enhancers in luciferase reporter assays. Gel retardation analysis demonstrated that two liver-enriched transcription factors, hepatocyte nuclear factor 4 (HNF4) and CCAAT enhancer-binding protein (C/EBPa), bound to the 5' upstream element. HNF4 bound to elements A and C, whereas C/EBPa bound to element B. Element A also interacted with the ubiquitous nuclear hormone receptors chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1), thyroid hormone receptor alpha (TRalpha), peroxisome proliferator-activated receptor alpha(PPARalpha), and retinoid X receptor alpha (RXRalpha). In HepG2 and BSC40 cells, HNF4, C/EBPalpha, and RXRalpha activated luciferase expression from a reporter construct containing the 5'-upstream minimal antithrombin gene promoter, while COUP-TF1, TRalpha, and HNF3 (alpha or beta) repressed such expression. Our results show that constitutive expression of the human antithrombin gene depends in part upon the interplay of these transcription factors and suggest that signaling pathways regulated by these factors can modulate antithrombin gene transcription. PMID:8910619

  5. Sulfamate proton solvent exchange in heparin oligosaccharides: evidence for a persistent hydrogen bond in the antithrombin-binding pentasaccharide Arixtra.

    PubMed

    Langeslay, Derek J; Young, Robert P; Beni, Szabolcs; Beecher, Consuelo N; Mueller, Leonard J; Larive, Cynthia K

    2012-09-01

    Sulfamate groups (NHSO(3)(-)) are important structural elements in the glycosaminoglycans (GAGs) heparin and heparan sulfate (HS). In this work, proton nuclear magnetic resonance (NMR) line-shape analysis is used to explore the solvent exchange properties of the sulfamate NH groups within heparin-related mono-, di-, tetra- and pentasaccharides as a function of pH and temperature. The results of these experiments identified a persistent hydrogen bond within the Arixtra (fondaparinux sodium) pentasaccharide between the internal glucosamine sulfamate NH and the adjacent 3-O-sulfo group. This discovery provides new insights into the solution structure of the Arixtra pentasaccharide and suggests that 3-O-sulfation of the heparin N-sulfoglucosamine (GlcNS) residues pre-organize the secondary structure in a way that facilitates binding to antithrombin-III. NMR studies of the GlcNS NH groups can provide important information about heparin structure complementary to that available from NMR spectral analysis of the carbon-bound protons. PMID:22593556

  6. Mechanism of poly(acrylic acid) acceleration of antithrombin inhibition of thrombin: implications for the design of novel heparin mimics.

    PubMed

    Monien, Bernhard H; Cheang, Kai I; Desai, Umesh R

    2005-08-11

    The bridging mechanism of antithrombin inhibition of thrombin is a dominant mechanism contributing a massive approximately 2500-fold acceleration in the reaction rate and is also a key reason for the clinical usage of heparin. Our recent study of the antithrombin-activating properties of a carboxylic acid-based polymer, poly(acrylic acid) (PAA), demonstrated a surprisingly high acceleration in thrombin inhibition (Monien, B. H.; Desai, U. R. J. Med. Chem. 2005, 48, 1269). To better understand this interesting phenomenon, we have studied the mechanism of PAA-dependent acceleration in antithrombin inhibition of thrombin. Competitive binding studies with low-affinity heparin and a heparin tetrasaccharide suggest that PAA binds antithrombin in both the pentasaccharide- and the extended heparin-binding sites, and these results are corroborated by molecular modeling. The salt-dependence of the K(D) of the PAA-antithrombin interaction shows the formation of five ionic interactions. In contrast, the contribution of nonionic forces is miniscule, resulting in an interaction that is significantly weaker than that observed for heparins. A bell-shaped profile of the observed rate constant for antithrombin inhibition of thrombin as a function of PAA concentration was observed, suggesting that inhibition proceeds through the "bridging" mechanism. The knowledge gained in this mechanistic study highlights important rules for the rational design of orally available heparin mimics. PMID:16078853

  7. Inhibition of thrombin generation in plasma by fibrin formation (Antithrombin I).

    PubMed

    de Bosch, N B; Mosesson, M W; Ruiz-Sáez, A; Echenagucia, M; Rodriguez-Lemoin, A

    2002-08-01

    The adsorption of thrombin to fibrin during clotting defines "Antithrombin I" activity. We confirmed that thrombin generation in afibrinogenemic or in Reptilase defibrinated normal plasma was higher than in normal plasma. Repletion of these fibrinogen-deficient plasmas with fibrinogen 1 (gamma A/gamma A), whose fibrin has two "low affinity" non-substrate thrombin binding sites, resulted in moderately reduced thrombin generation by 29-37%. Repletion with fibrinogen 2 (gamma'/gamma A), which in addition to low affinity thrombin-binding sites in fibrin, has a "high affinity" non-substrate thrombin binding site in the carboxy-terminal region of its gamma' chain, was even more effective and reduced thrombin generation by 57-67%. Adding peptides that compete for thrombin binding to fibrin [S-Hir53-64 (hirugen) or gamma'414-427] caused a transient delay in the onset of otherwise robust thrombin generation, indicating that fibrin formation is necessary for full expression of Antithrombin I activity. Considered together, 1) the increased thrombin generation in afibrinogenemic or fibrinogen-depleted normal plasma that is mitigated by fibrinogen replacement; 2) evidence that prothrombin activation is increased in afibrinogenemia and normalized by fibrinogen replacement; 3) the severe thrombophilia that is associated with defective thrombin-binding in dysfibrinogenemias Naples I and New York I, and 4) the association of afibrinogenemia or hypofibrinogenemia with venous or arterial thromboembolism, indicate that Antithrombin I (fibrin) modulates thromboembolic potential by inhibiting thrombin generation in blood. PMID:12195697

  8. Cerebral venous sinus thrombosis in the patient with multiple sclerosis associated with congenital antithrombin deficiency.

    PubMed

    Kanaya, Yuhei; Takamatsu, Kazuhiro; Shimoe, Yutaka; Niimi, Hideki; Kitajima, Isao; Kuriyama, Masaru

    2016-04-28

    We report the case of a 25-year-old man with multiple sclerosis (MS) who had severe headache and unconsciousness. He suffered from optic neuritis that had started at age 6. From the age of 12 years, he had suffered from multiple sclerosis (MS) cerebral lesions that relapsed three times over for 5 years. At age 25, he showed a new lesion in the cerebellar cortex, suggesting an exacerbation of the MS. However, magnetic resonance imaging findings the next day showed cerebral venous sinus thrombosis. His laboratory findings showed low antithrombin activity. Genetic analysis revealed a single-base substitution (C>T) at the codon 359 (Arg to STOP) in the 5th exon portion of the antithrombin gene, heterozygote. In the literature review, 17 cases of multiple sclerosis associated with cerebral venous sinus thrombosis, which occurred after the lumbar puncture and the treatment with high-dose methylpredonisolone in 11 of these cases. In our case, antithrombin deficiency, hyperhomocystinemia, infection, and lumbar puncture were suggested as the risk factors. PMID:27010094

  9. Molecular mechanisms of antithrombin-heparin regulation of blood clotting proteinases. a paradigm for understanding proteinase regulation by serpin family protein proteinase inhibitors

    PubMed Central

    Olson, Steven T.; Richard, Benjamin; Izaguirre, Gonzalo; Schedin-Weiss, Sophia; Gettins, Peter G. W.

    2010-01-01

    Serpin family protein proteinase inhibitors regulate the activity of serine and cysteine proteinases by a novel conformational trapping mechanism that may itself be regulated by cofactors to provide a finely-tuned time and location-dependent control of proteinase activity. The serpin, antithrombin, together with its cofactors, heparin and heparan sulfate, perform a critical anticoagulant function by preventing the activation of blood clotting proteinases except when needed at the site of a vascular injury. Here, we review the detailed molecular understanding of this regulatory mechanism that has emerged from numerous X-ray crystal structures of antithrombin and its complexes with heparin and target proteinases together with mutagenesis and functional studies of heparin-antithrombin-proteinase interactions in solution. Like other serpins, antithrombin achieves specificity for its target blood clotting proteinases by presenting recognition determinants in an exposed reactive center loop as well as in exosites outside the loop. Antithrombin reactivity is repressed in the absence of its activator because of unfavorable interactions that diminish the favorable RCL and exosite interactions with proteinases. Binding of a specific heparin or heparan sulfate pentasaccharide to antithrombin induces allosteric activating changes that mitigate the unfavorable interactions and promote template bridging of the serpin and proteinase. Antithrombin has thus evolved a sophisticated means of regulating the activity of blood clotting proteinases in a time and location-dependent manner that exploits the multiple conformational states of the serpin and their differential stabilization by glycosaminoglycan cofactors. PMID:20685328

  10. Dynamic properties of the native free antithrombin from molecular dynamics simulations: computational evidence for solvent- exposed Arg393 side chain.

    PubMed

    Tóth, László; Fekete, Attila; Balogh, Gábor; Bereczky, Zsuzsanna; Komáromi, István

    2015-09-01

    While antithrombin (AT) has small basal inhibitory activity, it reaches its full inhibitory potential against activated blood coagulation factors, FXa, FIXa, and FIIa (thrombin), via an allosteric and/or template (bridging) mechanism by the action of heparin, heparan sulfate, or heparin-mimetic pentasaccharides (PS). From the numerous X-ray structures available for different conformational states of AT, only indirect and incomplete conclusions can be drawn on the inherently dynamic properties of AT. As a typical example, the basal inhibitory activity of AT cannot be interpreted on the basis of "non-activated" free antithrombin X-ray structures since the Arg393 side chain, playing crucial role in antithrombin-proteinase interaction, is not exposed. In order to reveal the intrinsic dynamic properties and the reason of basal inhibitory activity of antithrombin, 2 μs molecular dynamics simulations were carried out on its native free-forms. It was shown from the simulation trajectories that the reactive center loop which is functioning as "bait" for proteases, even without any biasing potential can populate conformational state in which the Arg393 side chain is solvent exposed. It is revealed from the trajectory analysis that the peptide sequences correspond to the helix D extension, and new helix P formation can be featured with especially large root-mean-square fluctuations. Mutual information analyses of the trajectory showed remarkable (generalized) correlation between those regions of antithrombin which changed their conformations as the consequence of AT-PS complex formation. This suggests that allosteric information propagation pathways are present even in the non-activated native form of AT. PMID:25483839

  11. Effects of Preoperative Glycyrrhizin Infusion for the Prevention of Venous Thrombosis on the Tissue Expression of Antithrombin in a Rat Model

    PubMed Central

    Kira, Yukimi

    2016-01-01

    Objective: Using a thrombus model prepared by ligation of the inferior vena cava (IVC), the influences of the glycoside, glycyrrhizin, on plasma antithrombin levels and antithrombin mRNA expression levels in the liver and IVC with the inhibition of venous thrombosis were investigated. Materials and Methods: The rat IVC was exposed and ligated for 24 h immediately after the intravenous administration of 300 mg/kg glycyrrhizin. Among antithrombotic drugs, the Xa inhibitor, fondaparinux sodium, was used as a control drug. Results: The mean thrombus weight was significantly smaller in the glycyrrhizin-treated group (18.3 mg) than in the saline-treated group (34.3 mg). In contrast, the inhibition of thrombosis was not observed in the fondaparinux-treated group. Antithrombin mRNA expression levels in the liver were significantly higher in the ligated groups than in the baseline control group. The mean plasma antithrombin level was significantly lower in the glycyrrhizin group (96.6%) than in the saline group (114.4%), but was not significantly different from that in the baseline control group (102.4%). Conclusion: The pretreatment with glycyrrhizin inhibited venous thrombosis, and antithrombin mRNA expression levels in the liver and IVC as well as plasma antithrombin levels were significantly lower than those in the saline group. PMID:27375802

  12. Molecular-size-dependent variations in the proportions of chains with high binding affinities for antithrombin in rat skin heparin proteoglycans.

    PubMed Central

    Horner, A A

    1989-01-01

    Approximately half of all rat skin heparin proteoglycans have polysaccharide chains that have no sites with high binding affinity for antithrombin. The rest have chains with high-affinity antithrombin-binding-site densities ranging from zero to five sites per chain, with a high degree of variation. Proteoglycans vary in size because of diversity in the number of chains per molecule; the relationship between proteoglycan size and high-affinity antithrombin-binding-site density has not been studied previously. Polydisperse heparin proteoglycans from rat skin, labelled biosynthetically with 35S, were fractionated by gel filtration on Bio-Gel A-150m and arbitrarily divided into five fractions of decreasing average molecular size. Fractionation of these products on antithrombin-agarose showed that the proportion of proteoglycans with high affinity for antithrombin decreased from 39% to 25% as molecular size decreased. However, as the molecular size of high-affinity proteoglycans decreased, the proportion of their chains that had high affinity increased from 29% to 59%. Therefore molecular size is a significant factor in determining the proportion of high-affinity chains in heparin proteoglycans. A model of heparin biosynthesis is proposed in which areas of specific enzyme activity that control the synthesis of the antithrombin-binding-site sequence are sparsely and nonrandomly distributed on mast-cell Golgi membranes. It is postulated that the likelihood of a developing proteoglycan encountering one of these hypothetical areas is molecular-size-dependent. Images Fig. 1. PMID:2590178

  13. A recurrent deletion in the antithrombin gene, AT106-108(-6 bp), identified by DNA heteroduplex detection

    SciTech Connect

    Olds, R.J.; Thein, S.L. ); Lane, D.A. ); Beresford, C.H.; Hughes, P.M. ); Abildgaard, U. )

    1993-04-01

    Antithrombin is the major physiological inhibitor of the activated serine proteinases of the coagulation system. Hereditary deficiency of the inhibitor is transmitted in an autosomal dominant pattern and is associated with a risk of venous thromboembotic disease in affected individuals. In the classical form of deficiency, type Ia, plasma antithrombin is reduced to approximately half normal in both functional and immunological assays. The authors report here the identification of a recurrent mutation as the basis for type Ia deficiency in two independent kindreds, one from New Zealand and the other from Norway, and demonstrate the utility of DNA heteroduplex detection as a method for screening for the presence of mutations. Standard functional and immunological assays for plasma antithrombin showed levels of approximately half normal in several members of both kindreds, consistent with the classification as type Ia deficiency. The plasma of the proband from the Norwegian kindred was examined by crossed immunoelectrophoresis, in the presence or absence of heparin in the first dimension, and an abnormal component that may have represented a variant form of the inhibitor was not identified. In both families affected members have had episodes of venous thrombosis, although some carriers of the abnormal allele, as confirmed in the current study, so far have not had clinical thrombotic disease. 7 refs., 2 figs.

  14. Basement-membrane heparan sulphate with high affinity for antithrombin synthesized by normal and transformed mouse mammary epithelial cells.

    PubMed Central

    Pejler, G; David, G

    1987-01-01

    Basement-membrane proteoglycans, biosynthetically labelled with [35S]sulphate, were isolated from normal and transformed mouse mammary epithelial cells. Proteoglycans synthesized by normal cells contained mainly heparan sulphate and, in addition, small amounts of chondroitin sulphate chains, whereas transformed cells synthesized a relatively higher proportion of chondroitin sulphate. Polysaccharide chains from transformed cells were of lower average Mr and of lower anionic charge density compared with chains isolated from the untransformed counterparts, confirming results reported previously [David & Van den Berghe (1983) J. Biol. Chem. 258, 7338-7344]. A large proportion of the chains isolated from normal cells bound with high affinity to immobilized antithrombin, and the presence of 3-O-sulphated glucosamine residues, previously identified as unique markers for the antithrombin-binding region of heparin [Lindahl, Bäckström, Thunberg & Leder (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 6551-6555], could be demonstrated. A significantly lower proportion of the chains derived from transformed cells bound with high affinity to antithrombin, and a corresponding decrease in the amount of incorporated 3-O-sulphate was observed. PMID:2963617

  15. Structural peculiarity and antithrombin binding region profile of mucosal bovine and porcine heparins.

    PubMed

    Naggi, Annamaria; Gardini, Cristina; Pedrinola, Giacomo; Mauri, Lucio; Urso, Elena; Alekseeva, Anna; Casu, Benito; Cassinelli, Giuseppe; Guerrini, Marco; Iacomini, Marcello; Baigorria, Valentina; Torri, Giangiacomo

    2016-01-25

    The major compositional differences between bovine mucosal heparin (BMH) and the currently employed porcine mucosal heparin (PMH) have been reported to essentially consist of reduced 6-O-sulfation of the glucosamine residues in BMH and somewhat lower 2-O-sulfation of the iduronate residues in PMH. The present work is based on direct comparison of several BMH and PMH commercial preparations. A combined study by 2D (heteronuclear single quantum coherence, HSQC) NMR and ion-pair reversed-phase high performance liquid chromatography (IPRP-HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) on the heparins, extended to the analysis of their heparinases digests and fractions separated by affinity chromatography on antithrombin (AT), confirmed the previously reported lower degree of 6-O-sulfation and showed lower 3-O-sulfated glucosamine content in BMH. More detailed studies allowed the identification of structural variants of AT-binding region (ATBR) structural variants, showing higher content of the N-sulfated components in BMH than in PMH. PMID:26512999

  16. A capillary zone electrophoresis method to detect conformers and dimers of antithrombin in therapeutic preparations.

    PubMed

    Marie, Anne-Lise; Tran, Nguyet Thuy; Saller, François; Abdou, Youmna Mohamed; Zeau, Pascal; Plantier, Jean-Luc; Urbain, Rémi; Borgel, Delphine; Taverna, Myriam

    2016-07-01

    Antithrombin (AT) is a human plasma glycoprotein that possesses anticoagulant and anti-inflammatory properties. However, the native (active) form of AT is unstable and undergoes conformational changes, leading to latent, cleaved, and heterodimeric forms. The presence of these alternative forms mostly inactive can highly impact the quality and therapeutic activity of pharmaceutical AT preparations. We developed a capillary zone electrophoresis method, based on a neutral polyethylene oxide-coated capillary and a buffer close to physiological conditions, enabling the separation of more than eight forms of AT. Several peaks were identified as native, latent, and heterodimeric forms. The CZE method was reproducible with intraday relative standard deviations less than 0.5 and 2% for migration times and peak areas, respectively. The method was applied to the comparison of AT preparations produced by five competitive pharmaceutical companies, and statistical tests were performed. Important differences in the proportion of each form were highlighted. In particular, one AT preparation was shown to contain a high quantity of heterodimer, and two preparations contained high quantities of latent form. In addition, one AT preparation exhibited additional forms, not yet identified. PMID:26989842

  17. Different duplex/quadruplex junctions determine the properties of anti-thrombin aptamers with mixed folding.

    PubMed

    Russo Krauss, Irene; Spiridonova, Vera; Pica, Andrea; Napolitano, Valeria; Sica, Filomena

    2016-01-29

    Mixed duplex/quadruplex oligonucleotides have attracted great interest as therapeutic targets as well as effective biomedical aptamers. In the case of thrombin-binding aptamer (TBA), the addition of a duplex motif to the G-quadruplex module improves the aptamer resistance to biodegradation and the affinity for thrombin. In particular, the mixed oligonucleotide RE31 is significantly more effective than TBA in anticoagulation experiments and shows a slower disappearance rate in human plasma and blood. In the crystal structure of the complex with thrombin, RE31 adopts an elongated structure in which the duplex and quadruplex regions are perfectly stacked on top of each other, firmly connected by a well-structured junction. The lock-and-key shape complementarity between the TT loops of the G-quadruplex and the protein exosite I gives rise to the basic interaction that stabilizes the complex. However, our data suggest that the duplex motif may have an active role in determining the greater anti-thrombin activity in biological fluids with respect to TBA. This work gives new information on mixed oligonucleotides and highlights the importance of structural data on duplex/quadruplex junctions, which appear to be varied, unpredictable, and fundamental in determining the aptamer functional properties. PMID:26673709

  18. Different duplex/quadruplex junctions determine the properties of anti-thrombin aptamers with mixed folding

    PubMed Central

    Russo Krauss, Irene; Spiridonova, Vera; Pica, Andrea; Napolitano, Valeria; Sica, Filomena

    2016-01-01

    Mixed duplex/quadruplex oligonucleotides have attracted great interest as therapeutic targets as well as effective biomedical aptamers. In the case of thrombin-binding aptamer (TBA), the addition of a duplex motif to the G-quadruplex module improves the aptamer resistance to biodegradation and the affinity for thrombin. In particular, the mixed oligonucleotide RE31 is significantly more effective than TBA in anticoagulation experiments and shows a slower disappearance rate in human plasma and blood. In the crystal structure of the complex with thrombin, RE31 adopts an elongated structure in which the duplex and quadruplex regions are perfectly stacked on top of each other, firmly connected by a well-structured junction. The lock-and-key shape complementarity between the TT loops of the G-quadruplex and the protein exosite I gives rise to the basic interaction that stabilizes the complex. However, our data suggest that the duplex motif may have an active role in determining the greater anti-thrombin activity in biological fluids with respect to TBA. This work gives new information on mixed oligonucleotides and highlights the importance of structural data on duplex/quadruplex junctions, which appear to be varied, unpredictable, and fundamental in determining the aptamer functional properties. PMID:26673709

  19. Relationships of plasma factor VIIa-antithrombin complexes to manifest and future cardiovascular disease

    PubMed Central

    Silveira, Angela; Scanavini, Daniela; Boquist, Susanna; Ericsson, Carl-Göran; Hellénius, Mai-Lis; Leander, Karin; de Faire, Ulf; Öhrvik, John; Woodhams, Barry; Morrissey, James H.; Hamsten, Anders

    2011-01-01

    Background Low levels of free activated coagulation factor VII (VIIa) are normally present in plasma to prime the coagulation of blood in normal hemostasis and during thrombus formation. VIIa also circulates in inactive form, in complex with antithrombin (VIIaAT) formed when VIIa is bound to tissue factor (TF). This study evaluated VIIaAT in relation to cardiovascular disease (CVD). Methods We determined the plasma VIIaAT concentration in samples from the Stockholm Coronary Atherosclerosis Risk Factor (SCARF) study, a population-based case-control study of myocardial infarction (MI) and in samples from the Stockholm study of 60-years-old individuals, a prospective study of CVD. VIIaAT was measured with a sandwich ELISA that captures the complex between a monoclonal antibody to VIIa and a polyclonal antibody to AT. Results In the SCARF study (200 post-MI cases, 340 controls), VIIaAT was statistically significantly associated with patient status [odds ratio (95% confidence interval (CI)] 1.51 (1.09–2.08), p=0.0126). The case-control differences were however small, with VIIaAT values that largely overlap between the two groups. When a nested case-control design (211 incident CVD cases and 633 matched controls) was applied on 5- to 7-year follow-up results of the Stockholm prospective study of 60-year-olds, plasma VIIaAT concentration was not associated with incident CVD (odds ratio (95% CI) 1.001 (0.997–1.005), p=0.5447). Conclusions Plasma VIIaAT concentration had no predictive value for future CVD in our study population. Slightly increased plasma VIIaAT concentrations observed after MI may reflect processes that occur in connection with the acute event when TF and VIIa availability is increased. PMID:21925715

  20. Antithrombin Up-regulates AMP-activated Protein Kinase Signaling during Myocardial Ischemia/Reperfusion Injury

    PubMed Central

    Ma, Yina; Wang, Jinli; Gao, Junjie; Yang, Hui; Wang, Yanqing; Manithody, Chandrashekhara; Li, Ji; Rezaie, Alireza R.

    2014-01-01

    Summary Antithrombin (AT) is a protein of the serpin superfamily involved in regulation of the proteolytic activity of the serine proteases of the coagulation system. AT is known to exhibit anti-inflammatory and cardioprotective properties when it binds to heparan sulfate proteoglycans (HSPGs) on vascular cells. AMP-activated protein kinase (AMPK) plays an important cardioprotective role during myocardial ischemia and reperfusion (I/R). To determine whether the cardioprotective signaling function of AT is mediated through the AMPK pathway, we evaluated the cardioprotective activities of wild-type AT and its two derivatives, one having high affinity and the other no affinity for heparin, in an acute I/R injury model in C57BL/6J mice in which the left anterior descending coronary artery was occluded. The serpin derivatives were given 5 min before reperfusion. The results showed that AT-WT can activate AMPK in both in vivo and ex vivo conditions. Blocking AMPK activity abolished the cardioprotective function of AT against I/R injury. The AT derivative having high affinity for heparin was more effective in activating AMPK and in limiting infraction, but the derivative lacking affinity for heparin was inactive in eliciting AMPK-dependent cardioprotective activity. Activation of AMPK by AT inhibited the inflammatory c-Jun N-terminal protein kinase (JNK) pathway during I/R. Further studies revealed that the AMPK activity induced by AT also modulates cardiac substrate metabolism by increasing glucose oxidation but inhibiting fatty acid oxidation during I/R. These results suggest that AT binds to HSPGs on heart tissues to invoke a cardioprotective function by triggering cardiac AMPK activation, thereby attenuating JNK inflammatory signaling pathways and modulating substrate metabolism during I/R. PMID:25230600

  1. Biological Variations of Lupus Anticoagulant, Antithrombin, Protein C, Protein S, and von Willebrand Factor Assays.

    PubMed

    Shou, Weiling; Chen, Qian; Wu, Wei; Cui, Wei

    2016-02-01

    The results of lupus anticoagulant (LA), antithrombin (AT), protein C (PC), and protein S (PS) testing, and the values of von Willebrand factor antigen (VWF:Ag) are important in diagnosis and therapeutic monitoring of thrombosis and hemostasis diseases. Till now, no published study has focused on the biological variations in LA testing, and only a few studies have examined the biological variations of AT, PC, PS, and VWF:Ag. With the latest fully automated instruments and improved reagents, the analytical, within-subject, and between-subject biological variations were estimated for these five coagulant parameters in a cohort of 25 apparently healthy subjects. Blood specimens were collected at 8:00 am, 12:00 pm, and 4:00 pm on days 1, 3, and 5. The analytical biological variation (CV(A)) values of all the parameters were less than 3%. The within-subject biological variation (CV(W)) and between-subject biological variation (CV(G)) values of the LA normalized ratio were 4.64 and 6.83%, respectively. No significant differences were observed in the intraday and interday biological variations of LA tests, or in AT, PC, PS, and VWF:Ag values. Additionally, the utility of the conventional population-based reference intervals of the five coagulation parameters was evaluated by the index of individuality, and data on CV(W) and CV(A) were used to calculate the reference change value to identify the significance of changes in serial results from the same individual. PMID:26516946

  2. A Novel Serpin with Antithrombin-Like Activity in Branchiostoma japonicum: Implications for the Presence of a Primitive Coagulation System

    PubMed Central

    Chao, Yeqing; Fan, Chunxin; Liang, Yujun; Gao, Bei; Zhang, Shicui

    2012-01-01

    Serine protease inhibitors, or serpins, are a group of widely distributed proteins with similar structures that use conformational change to inhibit proteases. Antithrombin (AT) is a member of the serine protease inhibitor superfamily and a major coagulation inhibitor in all vertebrates, but its evolutionary origin remains elusive. In this study we isolated for the first time a cDNA encoding an antithrombin homolog, BjATl, from the protochordate Branchiostoma japonicum. The deduced protein BjATl consisted of 338 amino acids sharing 36.7% to 41.1% identity to known vertebrate ATs. BjATl contains a potential N-linked glycosylation site, two potential heparin binding sites and the reactive center loop with the absolutely conserved sequence Gly-Arg-Ser; all of these are features characteristic of ATs. All three phylogenetic trees constructed using Neighbor-Joining, Maximum-Likelihood and Bayesian-Inference methods also placed BjATl together with ATs. Moreover, BjATl expressed in yeast cells was able to inhibit bovine thrombin activity by forming a SDS-stable BjATl-thrombin complex. It also displays a concentration-dependent inhibition of thrombin that is accelerated by heparin. Furthermore, BjATl was predominantly expressed in the hepatic caecum and hind-gut, agreeing with the expression pattern of AT in mammalian species. All these data clearly demonstrate that BjATl is an ortholog of vertebrate ATs, suggesting that a primitive coagulation system emerged in the protochordate. PMID:22427833

  3. Localization of anticoagulantly active heparan sulfate proteoglycans in vascular endothelium: Antithrombin binding on cultured endothelial cells and perfused rat aorta

    SciTech Connect

    de Agostini, A.I.; Watkins, S.C.; Slayter, H.S.; Youssoufian, H.; Rosenberg, R.D. )

    1990-09-01

    We have studied the interaction of {sup 125}I-antithrombin ({sup 125}I-AT) with microvascular endothelial cells (RFPEC) to localize the cellular site of anticoagulantly active heparan sulfate proteoglycans (HSPG). The radiolabeled protease inhibitor bound specifically to the above HSPG with a Kd of approximately 50 nM. Confluent monolayer RFPEC cultures exhibited a linear increase in the amount of AT bound per cell for up to 16 d, whereas suspension RFPEC cultures possessed a constant number of protease inhibitor binding sites per cell for up to 5 d. These results suggest that monolayer RFPEC cultures secrete anticoagulantly active HSPG, which then accumulate in the extracellular matrix. This hypothesis was confirmed by quantitative light and EM level autoradiography which demonstrated that the AT binding sites are predominantly located in the extracellular matrix with only small quantities of protease inhibitor complexed to the cell surface. We have also pinpointed the in vivo position of anticoagulantly active HSPG within the blood vessel wall. Rat aortas were perfused, in situ, with {sup 125}I-AT, and bound labeled protease inhibitor was localized by light and EM autoradiography. The anticoagulantly active HSPG were concentrated immediately beneath the aortic and vasa vasorum endothelium with only a very small extent of labeling noted on the luminal surface of the endothelial cells. Based upon the above data, we propose a model whereby luminal and abluminal anticoagulantly active HSPG regulate coagulation mechanism activity.

  4. Comparison of biological activities of human antithrombins with high-mannose or complex-type nonfucosylated N-linked oligosaccharides.

    PubMed

    Yamada, Tsuyoshi; Kanda, Yutaka; Takayama, Makoto; Hashimoto, Akitoshi; Sugihara, Tsutomu; Satoh-Kubota, Ai; Suzuki-Takanami, Eri; Yano, Keiichi; Iida, Shigeru; Satoh, Mitsuo

    2016-05-01

    The structure of the N-linked oligosaccharides attached to antithrombin (AT) has been shown to affect its anticoagulant activity and pharmacokinetics. Human AT has biantennary complex-type oligosaccharides with the unique feature of lacking a core fucose, which affects its biological activities by changing its heparin-binding affinity. In human plasma, AT circulates as a mixture of the α-form bearing four oligosaccharides and the β-form lacking an oligosaccharide at Asn135. However, it remains unclear how the immature high-mannose-type oligosaccharides produced by mammalian cells affect biological activities of AT. Here, we succeeded in directly comparing the activities between the high-mannose and complex types. Interestingly, although there were no substantial differences in thrombin inhibitory activity, the high-mannose type showed higher heparin-binding affinity. The anticoagulant activities were increased by heparin and correlated with the heparin-binding affinity, resulting in the strongest anticoagulant activity being displayed in the β-form with the high-mannose type. In pharmacokinetic profiling, the high-mannose type showed a much shorter plasma half-life than the complex type. The β-form was found to have a prolonged plasma half-life compared with the α-form for the high-mannose type; conversely, the α-form showed a longer half-life than the β-form for the complex-type. The present study highlights that AT physiological activities are strictly controlled not only by a core fucose at the reducing end but also by the high-mannose-type structures at the nonreducing end. The β-form with the immature high-mannose type appears to function as a more potent anticoagulant than the AT typically found in human plasma, once it emerges in the blood. PMID:26747427

  5. Circulating microparticles and the risk of thrombosis in inherited deficiencies of antithrombin, protein C and protein S.

    PubMed

    Campello, Elena; Spiezia, Luca; Radu, Claudia M; Bulato, Cristiana; Gavasso, Sabrina; Tormene, Daniela; Woodhams, Barry; Dalla Valle, Fabio; Simioni, Paolo

    2016-01-01

    Many subjects carrying inherited thrombophilic defects will never experience venous thromboembolism (VTE) while other individuals developed recurrent VTE with no known additional risk factors. High levels of circulating microparticles (MP) have been associated with increased risk of VTE in patients with factor V Leiden and prothrombin G20210A mutation, suggesting a possible contribution of MP in the hypercoagulability of mild genetic thrombophilia. The role of MP as additional risk factor of VTE in carriers of natural clotting inhibitors defects (severe thrombophilia) has never been assessed. Plasma levels of annexin V-MP, endothelial-derived MP (EMP), platelet-derived MP (PMP), tissue factor-bearing MP (TF+) and the MP procoagulant activity (PPL) were measured in 132 carriers of natural anticoagulant deficiencies (25 antithrombin, 63 protein C and 64 protein S defect) and in 132 age and gender-matched healthy controls. Carriers of natural anticoagulant deficiencies, overall and separately considered, presented with higher median levels of annexin V-MP, EMP, PMP, TF+MP and PPL activity than healthy controls (p< 0.001, < 0.001, < 0.01, 0.025 and 0.03, respectively). Symptomatic carriers with a previous episode of VTE had significantly higher median levels of annexin-V MP than those without VTE (p=0.027). Carriers with high levels of annexin V-MP, EMP and PMP had an adjusted OR for VTE of 3.36 (95% CI, 1.59 to 7.11), 9.26 (95% CI, 3.55 to 24.1) and 2.72 (95%CI, 1.16 to 6.38), respectively. Elevated levels of circulating MP can play a role in carriers of mild and severe inherited thrombophilia. The clinical implications of this association remain to be defined. PMID:26354831

  6. ACRIM III

    Atmospheric Science Data Center

    2015-12-30

    ACRIM III Data and Information Active Cavity Radiometer Irradiance ... the ACRIMSAT spacecraft on December 20, 1999. ACRIM III data are reprocessed every 90 days to utilize instrument recalibration.   ... ACRIM III Instrument Team Page ACRIM II Data Sets SCAR-B Block:  SCAR-B Products ...

  7. Rat heparan sulphates. A study of the antithrombin-binding properties of heparan sulphate chains from rat adipose tissue, brain, carcase, heart, intestine, kidneys, liver, lungs, skin and spleen.

    PubMed Central

    Horner, A A

    1990-01-01

    Adult male rats were given [35S]sulphate intraperitoneally. Heparan [35S]sulphate (HS) chains were recovered from adipose tissue, brain, carcase, heart, intestine, kidneys, liver, lungs, skin and spleen by digestion with Pronase, precipitation with cetylpyridinium chloride, digestion with chondroitin ABC lyase and DNAase and gradient elution from DEAE-Sephacel. Purity was confirmed by agarose-gel electrophoresis and degradation with HNO2. Fractionation by gradient elution from antithrombin-agarose indicated that the proportion of HS with high binding affinity for antithrombin (HA-HS) ranged from 4.7% (kidneys) to 21.5% (brain). On a mass basis the major sources of HA-HS were carcase, skin and intestine. HA-HS from intestine was arbitrarily divided into subfractions I-VI, with anticoagulant activities ranging from 1 to 60 units/mg [by amidolytic anti-(Factor IIa) assay] and from 4 to 98 units/mg [by amidolytic anti-(Factor Xa) assay], indicating that the antithrombin-binding-site densities of HA-HS chains covered a wide range, as shown previously for rat HA-heparin chains [Horner, Kusche, Lindahl & Peterson (1988) Biochem. J. 251, 141-145]. HA-HS subfractions II, IV and VI were mixed with samples of HA-[3H]heparin chains and rechromatographed on antithrombin-agarose. Affinity for matrix-bound antithrombin did not correlate with anticoagulant activity, e.g. HA-HS subfraction IV [38 anti-(Factor Xa) units/mg] was co-eluted with HA-heparin chains [127 anti-(Factor Xa) units/mg]. Images Fig. 2. PMID:2138457

  8. SAGE III

    Atmospheric Science Data Center

    2016-06-15

    SAGE III Data and Information The Stratospheric Aerosol and Gas ... on the spacecraft. SAGE III produced L1 and L2 scientific data from 5/07/2002 until 12/31/2005. The flight of the second instrument is as ... Guide Documents:  Project Guide Data Products User's Guide  (PDF) Relevant Documents:  ...

  9. A neurodystrophic syndrome resembling carbohydrate-deficient glycoprotein syndrome type III.

    PubMed

    Stibler, H; Gylje, H; Uller, A

    1999-04-01

    A 10-month old girl is described with a serum transferrin isoform abnormality of the same kind as in two previously reported girls with carbohydrate-deficient glycoprotein syndrome type III. This patient presented with joint abnormalities and rapidly developing hypsarrhythmia, hypotonia, psychomotor delay and growth retardation. Fingers, toes, nails and local skin were dysmorphic. She had pale optic discs, thoracic syringomyelia and frontal lobe atrophy at three months. The CDT value in serum was greatly elevated. Several carbohydrate-deficient isoforms were found in transferrin (four), alpha1-antitrypsin (three), antithrombin (two) and thyroxine-binding globulin (four). Mutations in the CDGS 1-gene were excluded. The CDGS III glycoprotein abnormality most probably represents a distinct disorder of glycoprotein metabolism, and needs to be considered in unclear hypsarrhythmia with developmental delay. Dysmorphic features may be added to this syndrome. PMID:10401691

  10. Rat heparins. A study of the relative sizes and antithrombin-binding characteristics of heparin proteoglycans, chains and depolymerization products from rat adipose tissue, heart, lungs, peritoneal cavity and skin.

    PubMed Central

    Horner, A A

    1986-01-01

    35S-labelled heparins were recovered from adipose tissue, hearts, lungs, peritoneal cavities and skins of rats given H2(35)SO4. Their purification involved incubation with Pronase, precipitation with cetylpyridinium chloride in 1.0 M-NaCl, gradient elution from DEAE-Sephacel and incubation with chondroitinase ABC. Each product was divided into proteoglycan and "depolymerization products' fractions by gel filtration on Bio-Gel A-15m. Heparin chains were released from a portion of each proteoglycan fraction by beta-elimination with NaOH. Proteoglycans, chains and depolymerization products were separated by gradient elution from a column of antithrombin-agarose into fractions with no affinity, low affinity and high affinity for antithrombin. The relative sizes of the products were determined by gel filtration on columns of Bio-Gel A-50m, A-15m, A-1.5m and A-0.5m. Skin was the major source of heparin and contained the largest proteoglycans and the lowest proportion of depolymerization products. Lungs contained the smallest proteoglycans, the smallest depolymerization products and the highest proportion of depolymerization products. The highest proportions of proteoglycans, chains and depolymerization products with high affinity for antithrombin were found in adipose tissue. The lowest proportions of each of these fractions were found in the peritoneal cavity. The data suggest that there was relatively little biosynthesis of sites with high affinity for antithrombin in peritoneal-cavity mast cells and that heparin catabolism was most active in lungs. Each source of heparin was unique with respect to both biosynthesis and subsequent breakdown of its proteoglycans. PMID:3827837

  11. Welding III.

    ERIC Educational Resources Information Center

    Allegheny County Community Coll., Pittsburgh, PA.

    Instructional objectives and performance requirements are outlined in this course guide for Welding III, an advanced course in arc welding offered at the Community College of Allegheny County to provide students with the proficiency necessary for industrial certification. The course objectives, which are outlined first, specify that students will…

  12. LANDVIEW III

    EPA Science Inventory

    LandView III is a desktop mapping system that includes database extracts from the Environmental Protection Agency, the Bureau of the Census, The U.S. Geological Survey, the Nuclear Regulatory Commission, the Department of Transportation, and the Federal Emergency Management Agenc...

  13. A comparative evaluation of assays for markers of activated coagulation and/or fibrinolysis: thrombin-antithrombin complex, D-dimer and fibrinogen/fibrin fragment E antigen.

    PubMed

    Boisclair, M D; Lane, D A; Wilde, J T; Ireland, H; Preston, F E; Ofosu, F A

    1990-04-01

    Measurements were made of levels of D-dimer in plasma and serum, thrombin-antithrombin complex (TAT) in plasma and fibrinogen/fibrin fragment E antigen (FgE) in serum in a normal healthy control group and in patients with a range of disorders associated with hypercoagulability. Levels were determined in 31 normal healthy controls, 30 patients with disseminated intravascular coagulation (DIC), 21 patients with deep venous thrombosis (DVT), 27 patients with myocardial infarction (MI), 26 patients with acute leukaemia and 56 patients with liver disease. Considering all subjects, significant correlations were established between the results of all assays. Notably high correlations (r greater than 0.9) were established between plasma and serum levels of D-dimer, between plasma levels of D-dimer and serum levels of FgE, and between serum levels of D-dimer and FgE. All assays showed very high discrimination (sensitivity) between the normal control group and patients with DIC (97-100%), but there were marked differences between the assays in sensitivity for DVT and MI. In general, the FgE assay was more sensitive than the D-dimer assay, whilst both the FgE and D-dimer assays were more sensitive than the TAT assay. The same trends were apparent in the capability of the assays to discriminate between the normal control group and patients with acute leukaemia and liver disease: disorders with an unknown prevalence of activation of coagulation/fibrinolysis. Our results indicated that measurements of fibrinogen/fibrin degradation products (FDPs) in serum were almost unaffected by artefacts. The data further suggested that the broad-spectrum FgE assay was better than the more specific D-dimer assay in detecting clinical hypercoagulability. Our study showed that, in the clinical conditions examined, FDPs were more effective markers of hypercoagulability than TAT. PMID:2189490

  14. Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.

    PubMed

    Mourier, Pierre A J; Herman, Fréderic; Sizun, Philippe; Viskov, Christian

    2016-09-10

    Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism. The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors. Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity. The manufacturing process itself is associated with the introduction of exogenous microheterogeneities that may further affect its biological efficacy. This is important since enoxaparin is prepared by depolymerizing the heparin with the aim of optimizing its biological activity and safety. Changes during its manufacture could thus affect its biological activity and safety. The current study was performed to assess potential differences between the originator enoxaparin and a new generic enoxaparin commercialized by Teva. Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used. The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin. These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade. This study, together with other published analytical reports, describes specific compositional differences between generics and originator LWMHs. However, it is yet to be established whether such variations might have any clinical relevance. PMID:27497655

  15. Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy.

    PubMed

    Gleeson, Birgitta M; Martin, Kenneth; Ali, Mohammed T; Kumar, Arun H S; Pillai, M Gopala-Krishnan; Kumar, Sujith P G; O'Sullivan, John F; Whelan, Derek; Stocca, Alessia; Khider, Wisam; Barry, Frank P; O'Brien, Timothy; Caplice, Noel M

    2015-09-01

    Mesenchymal stem cells (MSCs) are currently under investigation as tools to preserve cardiac structure and function following acute myocardial infarction (AMI). However, concerns have emerged regarding safety of acute intracoronary (IC) MSC delivery. This study aimed to characterize innate prothrombotic activity of MSC and identify means of its mitigation toward safe and efficacious therapeutic IC MSC delivery post-AMI. Expression of the initiator of the coagulation cascade tissue factor (TF) on MSC was detected and quantified by immunofluorescence, FACS, and immunoblotting. MSC-derived TF antigen was catalytically active and capable of supporting thrombin generation in vitro. Addition of MSCs to whole citrated blood enhanced platelet thrombus deposition on collagen at arterial shear, an effect abolished by heparin coadministration. In a porcine AMI model, IC infusion of 25 × 10(6) MSC during reperfusion was associated with a decrease in coronary flow reserve but not when coadministered with an antithrombin agent (heparin). Heparin reduced MSC-associated thrombosis incorporating platelets and VWF within the microvasculature. Heparin-assisted therapeutic MSC delivery also reduced apoptosis in the infarct border zone at 24 hours, significantly improved infarct size, left ventricular (LV) ejection fraction, LV volumes, wall motion, and attenuated histologic evidence of scar formation at 6 weeks post-AMI. Heparin alone or heparin-assisted fibroblast control cell delivery had no such effect. Procoagulant TF activity of therapeutic MSCs is associated with reductions in myocardial perfusion when delivered IC may be successfully managed by heparin coadministration. This study highlights an important mechanistic insight into safety concerns associated with therapeutic IC MSC delivery for AMI. PMID:25969127

  16. Biopsy Quantitative Patohistology and Seral Values of Prostate Specific Antigen-Alpha (1) Antichymotrypsine Complex in Prediction of Adverse Pathology Findings after Radical Prostatectomy.

    PubMed

    Tomasković, Igor; Milicić, Valerija; Tomić, Miroslav; Ruzić, Boris; Ulamec, Monika

    2015-09-01

    In this prospective study we examined the utility of parameters obtained on prostate needle biopsy and prostate specific antigen-alpha(1)-antichymotripsine complex (PSA-ACT) to predict adverse pathologic findings after radical prostatectomy. 45 consecutive patients assigned for radical prostatectomy due to clinically localized prostate cancer were included in the study. Prostate biopsy parameters such as number of positive cores, the greatest percentage of tumor in the positive cores, Gleason score, perineural invasion, unilaterality or bilaterality of the tumor were recorded. PSA-ACT was determined using sandwich immunoassay chemiluminiscent method (Bayer, Tarrytown, New York). We analyzed relationship of preoperative PSA, PSA-ACTand quantitative biopsy parameters with final pathology after prostatectomy. Adverse findings were considered when extracapsular extension of cancer (pT3) was noted. Postoperatively, 29 (64.4%) patients were diagnosed with pT2 disease and 16 (35.6%) with pT3 disease. There was a significant difference in localized vs. locally advanced disease in number of positive biopsy cores (p<0.001), greatest percentage of tumor in the core (p=0.008), localization of the tumor (p=0.003) and perineural invasion (p=0.004). Logistic regression was used to develop a model on the multivariate level. It included number of positive cores and PSA-ACT and was significant on our cohort with the reliability of 82.22%. The combination of PSA-ACT and a large scale of biopsy parameters could be used in prediction of adverse pathologic findings after radical prostatectomy. Clinical decisions and patients counselling could be influenced by these predictors but further confirmation on a larger population is necessary. PMID:26898067

  17. SUPERSTARS III: K-2.

    ERIC Educational Resources Information Center

    North Carolina State Dept. of Public Education, Raleigh.

    SUPERSTARS III is a K-8 program designed as an enrichment opportunity for self-directed learners in mathematics. The basic purpose of SUPERSTARS III is to provide the extra challenge that self-motivated students need in mathematics and to do so in a structured, long-term program that does not impinge on the normal classroom routine or the…

  18. CITY III Player's Manual.

    ERIC Educational Resources Information Center

    Envirometrics, Inc., Washington, DC.

    CITY III is a computer-assisted simulation game in which participants make decisions affecting the economic, governmental, and social conditions of a simulated urban area. In CITY III, the computer stores all the relevant statistics for the area, updates data when changes are made, and prints out yearly reports. The computer also simulates…

  19. CITY III Operator's Manual.

    ERIC Educational Resources Information Center

    Envirometrics, Inc., Washington, DC.

    CITY III is a computer-assisted simulation game of an urban system involving player operation of and interaction with economic, social, and government components. The role of operator in the game is to take the handwritten inputs (decisions) from the CITY III participants, process them, and return output which initiates the next round of…

  20. Fusion Power Demonstration III

    SciTech Connect

    Lee, J.D.

    1985-07-01

    This is the third in the series of reports covering the Fusion Power Demonstration (FPD) design study. This volume considers the FPD-III configuration that incorporates an octopole end plug. As compared with the quadrupole end-plugged designs of FPD-I and FPD-II, this octopole configuration reduces the number of end cell magnets and shortens the minimum ignition length of the central cell. The end-cell plasma length is also reduced, which in turn reduces the size and cost of the end cell magnets and shielding. As a contiuation in the series of documents covering the FPD, this report does not stand alone as a design description of FPD-III. Design details of FPD-III subsystems that do not differ significantly from those of the FPD-II configuration are not duplicated in this report.

  1. Pioneer III Probe

    NASA Technical Reports Server (NTRS)

    1961-01-01

    Looking more like surgeons, these technicians wearing 'cleanroom' attire inspect the Pioneer III probe before shipping it to Cape Canaveral, Florida. Pioneer III was launched on December 6, 1958 aboard a Juno II rocket at the Atlantic Missile Range, Cape Canaveral, Florida. The mission objectives were to measure the radiation intensity of the Van Allen radiation belt, test long range communication systems, the launch vehicle and other subsystems. The Juno II failed to reach proper orbital escape velocity. The probe re-entered the Earth's atmosphere on December 7th ending its brief mission.

  2. Summary of Session III

    SciTech Connect

    Furman, M.A.

    2002-06-19

    This is a summary of the talks presented in Session III ''Simulations of Electron-Cloud Build Up'' of the Mini-Workshop on Electron-Cloud Simulations for Proton and Positron Beams ECLOUD-02, held at CERN, 15-18 April 2002.

  3. The Apple III.

    ERIC Educational Resources Information Center

    Ditlea, Steve

    1982-01-01

    Describes and evaluates the features, performance, peripheral devices, available software, and capabilities of the Apple III microcomputer. The computer's operating system, its hardware, and the commercially produced software it accepts are discussed. Specific applications programs for financial planning, accounting, and word processing are…

  4. CITY III Director's Guide.

    ERIC Educational Resources Information Center

    Envirometrics, Inc., Washington, DC.

    CITY III is a computer-assisted simulation game which allows the participants to make decisions affecting various aspects of the economic, governmental, and social sectors of a simulated urban area. The game director selects one of five possible starting city configurations, may set a number of conditions in the city before the start of play, and…

  5. Structural and functional characterization of cleavage and inactivation of human serine protease inhibitors by the bacterial SPATE protease EspPα from enterohemorrhagic E. coli.

    PubMed

    Weiss, André; Joerss, Hanna; Brockmeyer, Jens

    2014-01-01

    EspPα and EspI are serine protease autotransporters found in enterohemorrhagic Escherichia coli. They both belong to the SPATE autotransporter family and are believed to contribute to pathogenicity via proteolytic cleavage and inactivation of different key host proteins during infection. Here, we describe the specific cleavage and functional inactivation of serine protease inhibitors (serpins) by EspPα and compare this activity with the related SPATE EspI. Serpins are structurally related proteins that regulate vital protease cascades, such as blood coagulation and inflammatory host response. For the rapid determination of serpin cleavage sites, we applied direct MALDI-TOF-MS or ESI-FTMS analysis of coincubations of serpins and SPATE proteases and confirmed observed cleavage positions using in-gel-digest of SDS-PAGE-separated degradation products. Activities of both serpin and SPATE protease were assessed in a newly developed photometrical assay using chromogenic peptide substrates. EspPα cleaved the serpins α1-protease inhibitor (α1-PI), α1-antichymotrypsin, angiotensinogen, and α2-antiplasmin. Serpin cleavage led to loss of inhibitory function as demonstrated for α1-PI while EspPα activity was not affected. Notably, EspPα showed pronounced specificity and cleaved procoagulatory serpins such as α2-antiplasmin while the anticoagulatory antithrombin III was not affected. Together with recently published research, this underlines the interference of EspPα with hemostasis or inflammatory responses during infection, while the observed interaction of EspI with serpins is likely to be not physiologically relevant. EspPα-mediated serpin cleavage occurred always in flexible loops, indicating that this structural motif might be required for substrate recognition. PMID:25347319

  6. Structural and Functional Characterization of Cleavage and Inactivation of Human Serine Protease Inhibitors by the Bacterial SPATE Protease EspPα from Enterohemorrhagic E. coli

    PubMed Central

    Weiss, André; Joerss, Hanna; Brockmeyer, Jens

    2014-01-01

    EspPα and EspI are serine protease autotransporters found in enterohemorrhagic Escherichia coli. They both belong to the SPATE autotransporter family and are believed to contribute to pathogenicity via proteolytic cleavage and inactivation of different key host proteins during infection. Here, we describe the specific cleavage and functional inactivation of serine protease inhibitors (serpins) by EspPα and compare this activity with the related SPATE EspI. Serpins are structurally related proteins that regulate vital protease cascades, such as blood coagulation and inflammatory host response. For the rapid determination of serpin cleavage sites, we applied direct MALDI-TOF-MS or ESI-FTMS analysis of coincubations of serpins and SPATE proteases and confirmed observed cleavage positions using in-gel-digest of SDS-PAGE-separated degradation products. Activities of both serpin and SPATE protease were assessed in a newly developed photometrical assay using chromogenic peptide substrates. EspPα cleaved the serpins α1-protease inhibitor (α1-PI), α1-antichymotrypsin, angiotensinogen, and α2-antiplasmin. Serpin cleavage led to loss of inhibitory function as demonstrated for α1-PI while EspPα activity was not affected. Notably, EspPα showed pronounced specificity and cleaved procoagulatory serpins such as α2-antiplasmin while the anticoagulatory antithrombin III was not affected. Together with recently published research, this underlines the interference of EspPα with hemostasis or inflammatory responses during infection, while the observed interaction of EspI with serpins is likely to be not physiologically relevant. EspPα-mediated serpin cleavage occurred always in flexible loops, indicating that this structural motif might be required for substrate recognition. PMID:25347319

  7. Human plasma alpha-cysteine proteinase inhibitor. Purification by affinity chromatography, characterization and isolation of an active fragment.

    PubMed Central

    Gounaris, A D; Brown, M A; Barrett, A J

    1984-01-01

    Human plasma alpha-cysteine proteinase inhibitor (alpha CPI) was purified by a two-stage method: affinity chromatography on S-carboxymethyl-papain-Sepharose, and high-resolution anion-exchange chromatography. The protein was obtained as a form of Mr about 64 000 and material of higher Mr (about 100 000). In sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with reduction, both forms showed a major component of Mr 64 000. An antiserum was raised against alpha CPI, and 'rocket' immunoassays showed the mean concentration in sera from 19 individuals to be 35.9 mg/dl. Both low-Mr and high-Mr forms of alpha CPI were confirmed to be sialoglycoproteins by the decrease in electrophoretic mobility after treatment with neuraminidase. alpha CPI was shown immunologically to be distinct from antithrombin III and alpha 1-antichymotrypsin, two serine proteinase inhibitors from plasma with somewhat similar Mr values. alpha CPI was also distinct from cystatins A and B, the two intracellular low-Mr cysteine proteinase inhibitors from human liver. Complexes of alpha CPI with papain were detectable in immunoelectrophoresis, but dissociated to free enzyme and intact inhibitor in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The stoichiometry of binding of papain was close to 1:1 for both low-Mr and high-Mr forms. alpha CPI was found to be a tight-binding inhibitor of papain and human cathepsins H and L (Ki 34 pM, 1.1 nM and 62 pM respectively). By contrast, inhibition of cathepsin B was much weaker, Ki being about 35 microM. Dipeptidyl peptidase I also was weakly inhibited. Digestion of alpha CPI with bromelain gave rise to an inhibitory fragment of Mr about 22 000, which was isolated. Images Fig. 2. Fig. 3. Fig. 4. PMID:6548132

  8. Hyper III on ramp

    NASA Technical Reports Server (NTRS)

    1969-01-01

    The Hyper III was a full-scale lifting-body remotely piloted research vehicle (RPRV) built at what was then the NASA Flight Research Center located at Edwards Air Force Base in Southern California. The Flight Research Center (FRC--as Dryden was named from 1959 until 1976) already had experience with testing small-scale aircraft using model-airplane techniques, but the first true remotely piloted research vehicle was the Hyper III, which flew only once in December 1969. At that time, the Center was engaged in flight research with a variety of reentry shapes called lifting bodies, and there was a desire both to expand the flight research experience with maneuverable reentry vehicles, including a high-performance, variable-geometry craft, and to investigate a remotely piloted flight research technique that made maximum use of a research pilot's skill and experience by placing him 'in the loop' as if he were in the cockpit. (There have been, as yet, no female research pilots assigned to Dryden.) The Hyper III as originally conceived was a stiletto-shaped lifting body that had resulted from a study at NASA's Langley Research Center in Hampton, Virginia. It was one of a number of hypersonic, cross-range reentry vehicles studied at Langley. (Hypersonic means Mach 5--five times the speed of sound--or faster; cross-range means able to fly a considerable distance to the left or right of the initial reentry path.) The FRC added a small, deployable, skewed wing to compensate for the shape's extremely low glide ratio. Shop personnel built the 32-foot-long Hyper III and covered its tubular frame with dacron, aluminum, and fiberglass, for about $6,500. Hyper III employed the same '8-ball' attitude indicator developed for control-room use when flying the X-15, two model-airplane receivers to command the vehicle's hydraulic controls, and a telemetry system (surplus from the X-15 program) to transmit 12 channels of data to the ground not only for display and control but for data

  9. Fueling type III secretion

    PubMed Central

    Lee, Pei-Chung

    2015-01-01

    Type III secretion systems are complex nanomachines that export proteins from the bacterial cytoplasm across the cell envelope in a single step. They are at the core of the machinery used to assemble the bacterial flagellum, and the needle complex many Gram-negative pathogens use to inject effector proteins into host cells and cause disease. Several models have been put forward to explain how this export is energized, and the mechanism has been the subject of considerable debate. Here we present an overview of these models and discuss their relative merits. Recent evidence suggests that the proton motive force is the primary energy source for type III secretion, although contribution from refolding of secreted proteins has not been ruled out. The mechanism, by which the proton motive force is converted to protein export, remains enigmatic. PMID:25701111

  10. Cranial mononeuropathy III - diabetic type

    MedlinePlus

    ... gov/ency/article/000692.htm Cranial mononeuropathy III - diabetic type To use the sharing features on this page, please enable JavaScript. Cranial mononeuropathy III -- diabetic type -- is usually a complication of diabetes that causes ...

  11. Migration Type III

    NASA Astrophysics Data System (ADS)

    Artymowicz, Pawel

    2004-03-01

    Migration type IIIMigration of objects embedded in disks (and the accompanying eccentricity evolution) is becoming a major theme in planetary system formation.The underlying physics can be distilled into the notion of disk-planet coupling via Lindblad resonances, which launch waves, sometimes spectacular spiral shock waves in gas disks. The wave pattern exchanges angular momentum with the planet. That causes (i) migration, (ii) eccentricity evolution, and (iii) gap opening by sufficiently massive planets.A competing source of disk-planet interaction, the corotationaltorques, are much less conspicuous (corotation does not produce easilydetectable waves, as galaxy observers can attest) and have often been missed in the analysis of planet migration. If spiral waves are like waves at Goleta beach, then the corotation acts more like a stealthy riptide. Corotationalflows lie at the basis of a new, surprisingly rapid, mode of migration (type III),superseding the standard type II migration (with a gap), and revising the speed of type I migration (without a gap). The talk will contain results obtained at KITP, e.g., an analytical derivation of da/dt in type III motion. It will be illustrated by videos of high-resolution numerical simulations obtained with different implementations of the Piecewise Parabolic Method hydrodynamics.

  12. POPULATION III HYPERNOVAE

    SciTech Connect

    Smidt, Joseph; Whalen, Daniel J.; Wiggins, Brandon K.; Even, Wesley; Fryer, Chris L.; Johnson, Jarrett L.

    2014-12-20

    Population III supernovae have been of growing interest of late for their potential to directly probe the properties of the first stars, particularly the most energetic events that are visible near the edge of the observable universe. Until now, hypernovae, the unusually energetic Type Ib/c supernovae that are sometimes associated with gamma-ray bursts, have been overlooked as cosmic beacons at the highest redshifts. In this, the latest of a series of studies on Population III supernovae, we present numerical simulations of 25-50 M {sub ☉} hypernovae and their light curves done with the Los Alamos RAGE and SPECTRUM codes. We find that they will be visible at z = 10-15 to the James Webb Space Telescope and z = 4-5 to the Wide-Field Infrared Survey Telescope, tracing star formation rates in the first galaxies and at the end of cosmological reionization. If, however, the hypernova crashes into a dense shell ejected by its progenitor, it is expected that a superluminous event will occur that may be seen at z ∼ 20 in the first generation of stars.

  13. Ammonium diphosphitoindate(III)

    PubMed Central

    Hamchaoui, Farida; Rebbah, Houria; Le Fur, Eric

    2013-01-01

    The crystal structure of the title compound, NH4[In(HPO3)2], is built up from InIII cations (site symmetry 3m.) adopting an octa­hedral environment and two different phosphite anions (each with site symmetry 3m.) exhibiting a triangular–pyramidal geometry. Each InO6 octa­hedron shares its six apices with hydrogen phosphite groups. Reciprocally, each HPO3 group shares all its O atoms with three different metal cations, leading to [In(HPO3)2]− layers which propagate in the ab plane. The ammonium cation likewise has site symmetry 3m.. In the structure, the cations are located between the [In(HPO3)2]− layers of the host framework. The sheets are held together by hydrogen bonds formed between the NH4 + cations and the O atoms of the framework. PMID:23633983

  14. Ammonium diphosphitoindate(III).

    PubMed

    Hamchaoui, Farida; Rebbah, Houria; Le Fur, Eric

    2013-04-01

    The crystal structure of the title compound, NH4[In(HPO3)2], is built up from In(III) cations (site symmetry 3m.) adopting an octa-hedral environment and two different phosphite anions (each with site symmetry 3m.) exhibiting a triangular-pyramidal geometry. Each InO6 octa-hedron shares its six apices with hydrogen phosphite groups. Reciprocally, each HPO3 group shares all its O atoms with three different metal cations, leading to [In(HPO3)2](-) layers which propagate in the ab plane. The ammonium cation likewise has site symmetry 3m.. In the structure, the cations are located between the [In(HPO3)2](-) layers of the host framework. The sheets are held together by hydrogen bonds formed between the NH4 (+) cations and the O atoms of the framework. PMID:23633983

  15. Pseudo Class III malocclusion

    PubMed Central

    Al-Hummayani, Fadia M.

    2016-01-01

    The treatment of deep anterior crossbite is technically challenging due to the difficulty of placing traditional brackets with fixed appliances. This case report represents a none traditional treatment modality to treat deep anterior crossbite in an adult pseudo class III malocclusion complicated by severely retruded, supraerupted upper and lower incisors. Treatment was carried out in 2 phases. Phase I treatment was performed by removable appliance “modified Hawley appliance with inverted labial bow,” some modifications were carried out to it to suit the presented case. Positive overbite and overjet was accomplished in one month, in this phase with minimal forces exerted on the lower incisors. Whereas, phase II treatment was performed with fixed appliances (braces) to align teeth and have proper over bite and overjet and to close posterior open bite, this phase was accomplished within 11 month. PMID:27052290

  16. Force constants of phosphorus (III) cyanide and arsenic (III) cyanide

    NASA Astrophysics Data System (ADS)

    Edwards, H. G. M.; Fawcett, V.

    The force constants of phosphorus (III) cyanide and arsenic (III) cyanide have been calculated using a simple valence force-field approximation with interaction constants. Several revisions are proposed to the existing vibrational assignments for the As(CN) 3 species and the vibrational assignments for P(CN) 3 are confirmed.

  17. Title III in Special Education.

    ERIC Educational Resources Information Center

    The Title III Quarterly, 1972

    1972-01-01

    The journal on special education programs funded under Title III of the Elementary and Secondary Education Act contains articles on three projects, abstracts of other projects, a picture story on San Diego Schools' outdoor classroom for special education, and a state by state listing of all Title III special education projects. The programs…

  18. SUPERSTARS III: 3-5.

    ERIC Educational Resources Information Center

    North Carolina State Dept. of Public Education, Raleigh.

    SUPERSTARS III is a K-8 program designed as an enrichment opportunity for self-directed learners in mathematics. The basic purpose of SUPERSTARS III is to provide the extra challenge that self-motivated students need in mathematics and to do so in a structured, long-term program that does not impinge on the normal classroom routine or the…

  19. SUPERSTARS III: 6-8.

    ERIC Educational Resources Information Center

    North Carolina State Dept. of Public Education, Raleigh.

    SUPERSTARS III is a K-8 program designed as an enrichment opportunity for self-directed learners in mathematics. The basic purpose of SUPERSTARS III is to provide the extra challenge that self-motivated students need in mathematics and to do so in a structured, long-term program that does not impinge on the normal classroom routine or the…

  20. PREFACE: Quantum Optics III

    NASA Astrophysics Data System (ADS)

    Orszag, M.; Retamal, J. C.; Saavedra, C.; Wallentowitz, S.

    2007-06-01

    All the 50 years of conscious pondering did not bring me nearer to an answer to the question `what is light quanta?'. Nowadays, every rascal believes, he knows it, however, he is mistaken. (A Einstein, 1951 in a letter to M Besso) Quantum optics has played a key role in physics in the last several decades. On the other hand, in these early decades of the information age, the flow of information is becoming more and more central to our daily life. Thus, the related fields of quantum information theory as well as Bose-Einstein condensation have acquired tremendous importance in the last couple of decades. In Quantum Optics III, a fusion of these fields appears in a natural way. Quantum Optics III was held in Pucón, Chile, in 27-30 of November, 2006. This beautiful location in the south of Chile is near the lake Villarrica and below the snow covered volcano of the same name. This fantastic environment contributed to a relaxed atmosphere, suitable for informal discussion and for the students to have a chance to meet the key figures in the field. The previous Quantum Optics conferences took place in Santiago, Chile (Quantum Optics I, 2000) and Cozumel, Mexico (Quantum Optics II, 2004). About 115 participants from 19 countries attended and participated in the meeting to discuss a wide variety of topics such as quantum-information processing, experiments related to non-linear optics and squeezing, various aspects of entanglement including its sudden death, correlated twin-photon experiments, light storage, decoherence-free subspaces, Bose-Einstein condensation, discrete Wigner functions and many more. There was a strong Latin-American participation from Argentina, Brazil, Chile, Colombia, Peru, Uruguay, Venezuela and Mexico, as well as from Europe, USA, China, and Australia. New experimental and theoretical results were presented at the conference. In Latin-America a quiet revolution has taken place in the last twenty years. Several groups working in quantum optics and

  1. Cranial mononeuropathy III - diabetic type

    MedlinePlus

    Diabetic third nerve palsy; Pupil-sparing third cranial nerve palsy ... Cranial mononeuropathy III - diabetic type -- is a mononeuropathy . This means that only one nerve is damaged. The condition affects the third cranial (oculomotor) ...

  2. The MAX III storage ring

    NASA Astrophysics Data System (ADS)

    Sjöström, M.; Wallén, E.; Eriksson, M.; Lindgren, L.-J.

    2009-04-01

    One of the primary goals of the 700 MeV MAX III synchrotron radiation source is to test and gain experience with new magnet and accelerator technology. Each magnet cell is machined out of two solid iron blocks that are then sandwiched together after coil and quadrupole installation. The MAX III ring makes extensive use of combined function magnets to obtain a compact lattice. In order to obtain flexibility in machine tuning pole face current strips are used in the main dipoles, which also contain the horizontally defocusing gradients. Commissioning finished in 2007 and MAX III is now going into user operation. Over the last year, MAX III has been characterized in order to both obtain calibrated models for operation purposes as well as evaluating the magnet technology. The characterization results will be described in this paper.

  3. The START III bargaining space

    SciTech Connect

    Karas, T.H.

    1998-08-01

    The declining state of the Russian military and precarious Russian economic condition will give the US considerable advantages at the START III bargaining table. Taking the US-RF asymmetries into account, this paper discusses a menu of START III measures the US could ask for, and measures it could offer in return, in attempting to negotiate an equitable treaty. Measures the US might seek in a START III treaty include: further reductions in deployed strategic nuclear warheads, irreversibility of reductions through warhead dismantlement; beginning to bring theater nuclear weapons under mutual control, and increased transparency into the Russian nuclear weapons complex. The US may, however, wish to apply its bargaining advantages to attempting to achieve the first steps toward two long-range goals that would enhance US security: bringing theater nuclear weapons into the US-RF arms control arena, and increasing transparency into the Russian nuclear weapons complex. In exchange for measures relating to these objectives, the US might consider offering to Russia: Further strategic weapons reductions approaching levels at which the Russians believe they could maintain a degree of parity with the US; Measures to decrease the large disparities in potential deliver-system uploading capabilities that appear likely under current START II/START III scenarios; and Financial assistance in achieving START II/START III reductions as rapidly as is technically possible.

  4. Thermal and optical properties of Tb(III), Eu(III) and Tb(III)/Eu(III) co-complexed silicone fluorinated acrylate copolymer

    NASA Astrophysics Data System (ADS)

    Zhai, Yinfeng; Xie, Hongde; Cai, Haijun; Cai, Peiqing; Seo, Hyo Jin

    2015-07-01

    Tb(III), Eu(III) and Tb(III)/Eu(III) activated silicone fluorinated acrylate (SFA) have been successfully synthesized using the method of semi-continuous emulsion polymerization. The copolymers are characterized by flourier transform infrared (FT-IR), thermal gravity analysis (TGA), photoluminescence excitation (PLE) and emission (PL) spectroscopy. The copolymer containing Tb(III) and Eu(III) ions display green and red luminescent colors under UV light excitation, respectively. The TGA curves show the thermal decomposition temperatures of the copolymers are up to about 300 °C. The PL spectra show a strong green emission at 546 nm (5D4 → 7F5) of Tb(III) complexed copolymers, and show a prominent red emission at 615 nm (5D0 → 7F2) of Eu(III) complexed copolymers. Different concentrations of Eu(III) and Tb(III) ions are introduced into the copolymer and the energy transfer from Tb(III) to Eu(III) ions in the copolymer was found. Thus, based on the results it can be suggested that SFA:Eu(III), SFA:Tb(III) and SFA:Tb(III)/Eu(III) can be used potentially as luminescent materials.

  5. III-Nitride nanowire optoelectronics

    NASA Astrophysics Data System (ADS)

    Zhao, Songrui; Nguyen, Hieu P. T.; Kibria, Md. G.; Mi, Zetian

    2015-11-01

    Group-III nitride nanowire structures, including GaN, InN, AlN and their alloys, have been intensively studied in the past decade. Unique to this material system is that its energy bandgap can be tuned from the deep ultraviolet (~6.2 eV for AlN) to the near infrared (~0.65 eV for InN). In this article, we provide an overview on the recent progress made in III-nitride nanowire optoelectronic devices, including light emitting diodes, lasers, photodetectors, single photon sources, intraband devices, solar cells, and artificial photosynthesis. The present challenges and future prospects of III-nitride nanowire optoelectronic devices are also discussed.

  6. Organometallic neptunium(III) complexes.

    PubMed

    Dutkiewicz, Michał S; Farnaby, Joy H; Apostolidis, Christos; Colineau, Eric; Walter, Olaf; Magnani, Nicola; Gardiner, Michael G; Love, Jason B; Kaltsoyannis, Nikolas; Caciuffo, Roberto; Arnold, Polly L

    2016-08-01

    Studies of transuranic organometallic complexes provide a particularly valuable insight into covalent contributions to the metal-ligand bonding, in which the subtle differences between the transuranium actinide ions and their lighter lanthanide counterparts are of fundamental importance for the effective remediation of nuclear waste. Unlike the organometallic chemistry of uranium, which has focused strongly on U(III) and has seen some spectacular advances, that of the transuranics is significantly technically more challenging and has remained dormant. In the case of neptunium, it is limited mainly to Np(IV). Here we report the synthesis of three new Np(III) organometallic compounds and the characterization of their molecular and electronic structures. These studies suggest that Np(III) complexes could act as single-molecule magnets, and that the lower oxidation state of Np(II) is chemically accessible. In comparison with lanthanide analogues, significant d- and f-electron contributions to key Np(III) orbitals are observed, which shows that fundamental neptunium organometallic chemistry can provide new insights into the behaviour of f-elements. PMID:27442286

  7. Terrain Perception for DEMO III

    NASA Technical Reports Server (NTRS)

    Manduchi, R.; Bellutta, P.; Matthies, L.; Owens, K.; Rankin, A.

    2000-01-01

    The Demo III program has as its primary focus the development of autonomous mobility for a small rugged cross country vehicle. In this paper we report recent progress on both stereo-based obstacle detection and terrain cover color-based classification.

  8. Title III hazardous air pollutants

    SciTech Connect

    Todd, R.

    1995-12-31

    The author presents an overview of the key provisions of Title III of the Clean Air Act Amendments of 1990. The key provisions include the following: 112(b) -- 189 Hazardous Air Pollutants (HAP); 112(a) -- Major Source: 10 TPY/25 TPY; 112(d) -- Application of MACT; 112(g) -- Modifications; 112(I) -- State Program; 112(j) -- The Hammer; and 112(r) -- Accidental Release Provisions.

  9. Chromium(III), insoluble salts

    Integrated Risk Information System (IRIS)

    Chromium ( III ) , insoluble salts ; CASRN 16065 - 83 - 1 Human health assessment information on a chemical substance is included in the IRIS database only after a comprehensive review of toxicity data , as outlined in the IRIS assessment development process . Sections I ( Health Hazard Assessments

  10. Precipitates of Al(III), Sc(III), and La(III) at the muscovite-water interface.

    PubMed

    Saslow Gomez, Sarah A; Geiger, Franz M

    2014-11-20

    The interaction of Al(III), Sc(III), and La(III) with muscovite-water interfaces was studied at pH 4 and 10 mM NaCl using second harmonic generation (SHG) and X-ray photoelectron spectroscopy (XPS). SHG data for Sc(III) and La(III) suggest complete and/or partial irreversible adsorption that is attributed by XPS to the growth of Sc(III) and La(III) hydroxides/oxides on the muscovite surface. Al(III) adsorption appears to coincide with the growth of gibbsite (Al(OH)3) deposits on the muscovite surface, as indicated by the magnitude of the interfacial potential computed from the SHG data. This interpretation of the data is consistent with previous studies reporting the epitaxial growth of gibbsite on the muscovite surface under similar conditions. The implication of our findings is that the surface charge density of mica may change (and in the case of Al(III), even flip sign from negative (mica) to positive (gibbsite)) when Al(III), Sc(III), or La(III) is present in aqueous phases in contact with heterogeneous geochemical media rich in mica-class minerals, even at subsaturation conditions. PMID:25380548

  11. NIF Title III engineering plan

    SciTech Connect

    Deis, G

    1998-06-01

    The purpose of this document is to define the work that must be accomplished by the NIF Project during Title III Engineering. This definition is intended to be sufficiently detailed to provide a framework for yearly planning, to clearly identify the specific deliverables so that the Project teams can focus on them, and to provide a common set of objectives and processes across the Project. This plan has been preceded by similar documents for Title I and Title II design and complements the Site Management Plan, the Project Control Manual, the Quality Assurance Program Plan, the RM Parsons NIF Title III Configuration Control Plan, the Integrated Project Schedule, the Preliminary Safety Analysis Report, the Configuration Management Plan, and the Transition Plan.

  12. DSN tracking system: Mark III-77

    NASA Technical Reports Server (NTRS)

    Chaney, W. D.

    1977-01-01

    The Deep Space Network tracking system is described. Revisions of subsystem Mark III-75 are briefly outlined, and the currently used multimission support subsystem Mark III-77 is described. Tracking functions performed are given as well.

  13. Silver europium(III) polyphosphate

    PubMed Central

    Ayadi, Mounir; Férid, Mokhtar; Moine, Bernard

    2009-01-01

    Europium(III) silver polyphosphate, AgEu(PO3)4, was prepared by the flux method. The atomic arrangement is built up by infinite (PO3)n chains (periodicity of 4) extending along the c axis. These chains are joined to each other by EuO8 dodeca­hedra. The Ag+ cations are located in the voids of this arrangement and are surrounded by five oxygen atoms in a distorted [4+1] coordination. PMID:21582031

  14. Organometallic neptunium(III) complexes

    NASA Astrophysics Data System (ADS)

    Dutkiewicz, Michał S.; Farnaby, Joy H.; Apostolidis, Christos; Colineau, Eric; Walter, Olaf; Magnani, Nicola; Gardiner, Michael G.; Love, Jason B.; Kaltsoyannis, Nikolas; Caciuffo, Roberto; Arnold, Polly L.

    2016-08-01

    Studies of transuranic organometallic complexes provide a particularly valuable insight into covalent contributions to the metal–ligand bonding, in which the subtle differences between the transuranium actinide ions and their lighter lanthanide counterparts are of fundamental importance for the effective remediation of nuclear waste. Unlike the organometallic chemistry of uranium, which has focused strongly on UIII and has seen some spectacular advances, that of the transuranics is significantly technically more challenging and has remained dormant. In the case of neptunium, it is limited mainly to NpIV. Here we report the synthesis of three new NpIII organometallic compounds and the characterization of their molecular and electronic structures. These studies suggest that NpIII complexes could act as single-molecule magnets, and that the lower oxidation state of NpII is chemically accessible. In comparison with lanthanide analogues, significant d- and f-electron contributions to key NpIII orbitals are observed, which shows that fundamental neptunium organometallic chemistry can provide new insights into the behaviour of f-elements.

  15. Glycosaminoglycans and mucopolysaccharidosis type III.

    PubMed

    Jakobkiewicz-Banecka, Joanna; Gabig-Ciminska, Magdalena; Kloska, Anna; Malinowska, Marcelina; Piotrowska, Ewa; Banecka-Majkutewicz, Zyta; Banecki, Bogdan; Wegrzyn, Alicja; Wegrzyn, Grzegorz

    2016-01-01

    Mucopolysaccharidosis type III (MPS III), or Sanfilippo syndrome, is a lysosomal storage disease in which heparan sulfate is accumulated in lysosomes, as well as outside of cells, as the primary storage material. This disease is a complex of four conditions caused by dysfunctions of one of genes coding for lysosomal enzymes involved in degradation of heparan sulfate: SGSH (coding for heparan N-sulfatase) - causing MPS IIIA, NAGLU (coding for alpha-N-acetylglucosaminidase) - causing MPS IIIB, HGSNAT (coding for acetyl CoA alpha-glucosaminide acetyltransferase) - causing MPS IIIC), and GNS (coding for N-acetylglucosamine-6-sulfatase) - causing MPS IIID. The primary storage is responsible for some disease symptoms, but other arise as a result of secondary storage, including glycosphingolipids, and subsequent processes, like oxidative stress and neuroinflammation. Central nervous system is predominantly affected in all subtypes of MPS III. Heparan sulfate and its derivatives are the most commonly used biomarkers for diagnosis and prediction procedures. Currently, there is no therapy for Sanfilippo syndrome, however, clinical trials are ongoing for enzyme replacement therapy, gene therapy and substrate reduction therapy (particularly gene expression-targeted isoflavone therapy). PMID:27100513

  16. The gelation mechanism of chromium(III)

    SciTech Connect

    Shu, P.

    1988-05-01

    Chromium(III) is commonly used crosslinker for preparing profile control gels with polymers having carboxylate and amide functionalities. Cr(III) is applied in many forms. For example, it can be used in the form of simple chromic salts of chloride and sulfate, or as complexed Cr(III) used in leather tanning, or as in-situ generated Cr(III) from the redox reaction of dichromate and bisulfite or thiourea. The gelation rate, and gel quality, doped on which form of Cr(III) is used. The author has found that the Cr olates, produced by hydrolysis of Cr(III) ions, are the reactive crosslinking species. The different gelation rates are due to the different degrees of olation. Furthermore, by controlling the degree of hydrolysis Cr(III) derived from various sources mentioned above can exhibit the same gelation rate.

  17. Titan III-C Launch

    NASA Technical Reports Server (NTRS)

    1970-01-01

    This photograph shows a Titan III-C launch vehicle. Titan vehicles are designed to carry payloads equal to the size and weight of those on the space shuttle. The Titan IV Centaur can put 10,000 pound payloads into geosynchronous orbit, 22,300 miles above Earth. For more information about Titan and Centaur, please see chapters 4 and 8, respectively, in Roger Launius and Dennis Jenkins' book To Reach the High Frontier published by The University Press of Kentucky in 2002.

  18. The Mark III vertex chamber

    SciTech Connect

    Adler, J.; Bolton, T.; Bunnell, K.; Cassell, R.; Cheu, E.; Freese, T.; Grab, C.; Mazaheri, G.; Mir, R.; Odian, A.

    1987-07-01

    The design and construction of the new Mark III vertex chamber is described. Initial tests with cosmic rays prove the ability of track reconstruction and yield triplet resolutions below 50 ..mu..m at 3 atm using argon/ethane (50:50). Also performed are studies using a prototype of a pressurized wire vertex chamber with 8 mm diameter straw geometry. Spatial resolution of 35mm was obtained using dimethyl ether (DME) at 1 atm and 30 ..mu..m using argon/ethane (50/50 mixture) at 4 atm. Preliminary studies indicate the DME to adversely affect such materials as aluminized Mylar and Delrin.

  19. Implementing Title III -- Air toxics

    SciTech Connect

    Shaw, B.W.

    1995-12-31

    The South Coast Air Quality Management District (AQMD) is taking three basic approaches to implementing the new National Emissions Standards for Hazardous Air Pollutants (NESHAPs) from the Title III program: accept and implement, as written, the NESHAPs where few sources are located in the South Coast Air Basin; incorporate with simplification of the NESHAP requirements into AQMD rules when many sources are involved; then seek equivalency by the US EPA; and incorporate with a market-based rule (VOC RECLAIM), part of many NESHAPs which control volatile organic compound as HAPs. Whatever the approach, emphasis will be placed on: streamlining and simplification; helping sources understand requirements and comply; and common sense.

  20. Zinc in +III oxidation state

    NASA Astrophysics Data System (ADS)

    Samanta, Devleena; Jena, Puru

    2012-02-01

    The possibility of Group 12 elements, such as Zn, Cd, and Hg existing in an oxidation state of +III or higher has fascinated chemists for decades. Significant efforts have been made in the past to achieve higher oxidation states for the heavier congener mercury (since the 3^rd ionization potential of the elements decrease as we go down the periodic table). It took nearly 20 years before experiment could confirm the theoretical prediction that Hg indeed can exist in an oxidation state of +IV. While this unusual property of Hg is attributed to the relativistic effects, Zn being much lighter than Hg has not been expected to have an oxidation state higher than +II. Using density functional theory we show that an oxidation state of +III for Zn can be realized by choosing specific ligands with large electron affinities i.e. superhalogens. We demonstrate this by a systematic study of the interaction of Zn with F, BO2, and AuF6 ligands whose electron affinities are progressively higher, namely, 3.4 eV, 4.4 eV, and 8.4 eV, respectively. Discovery of higher oxidation states of elements can help in the formulation of new reactions and hence in the development of new chemistry.

  1. DOE/NNSA perspective safeguard by design: GEN III/III+ light water reactors and beyond

    SciTech Connect

    Pan, Paul Y

    2010-12-10

    An overview of key issues relevant to safeguards by design (SBD) for GEN III/IV nuclear reactors is provided. Lessons learned from construction of typical GEN III+ water reactors with respect to SBD are highlighted. Details of SBD for safeguards guidance development for GEN III/III+ light water reactors are developed and reported. This paper also identifies technical challenges to extend SBD including proliferation resistance methodologies to other GEN III/III+ reactors (except HWRs) and GEN IV reactors because of their immaturity in designs.

  2. Dens invaginatus (Type III B)

    PubMed Central

    Kallianpur, Shreenivas; Sudheendra, US; Kasetty, Sowmya; Joshi, Prathamesh

    2012-01-01

    Dens invaginatus or ‘dens in dente’ is a developmental malformation of the tooth resulting from infolding of the dental papilla before calcification. This article presents a case of dens invaginatus occurring in maxillary right lateral incisor of a 45-year-old male patient. The patient presented with pain and clinically missing maxillary right canine. The tooth was found to be non-vital. Radiographic examination revealed the tooth-in-tooth appearance of lateral incisor with a dilated pulp chamber. The crown of impacted canine was found within the pulp chamber of lateral incisor. Owing to this unique clinical presentation, both the lateral incisor and the impacted canine were extracted. Histopathologic examination confirmed the diagnosis of Dens invaginatus Type III B. A brief review on etiopathogenesis, radiographic features and treatment of dens invaginatus has also been included. PMID:22923901

  3. Comparative adsorption of Eu(III) and Am(III) on TPD.

    PubMed

    Fan, Q H; Zhao, X L; Ma, X X; Yang, Y B; Wu, W S; Zheng, G D; Wang, D L

    2015-09-01

    Comparative adsorption behaviors of Eu(III) and Am(III) on thorium phosphate diphosphate (TPD), i.e., Th4(PO4)4P2O7, have been studied using a batch approach and surface complexation model (SCM) in this study. The results showed that Eu(III) and Am(III) adsorption increased to a large extent with the increase in TPD dose. Strong pH-dependence was observed in both Eu(III) and Am(III) adsorption processes, suggesting that inner-sphere complexes (ISCs) were possibly responsible for the adsorption of Eu(III) and Am(III). Meanwhile, the adsorption of Eu(III) and Am(III) decreased to a different extent with the increase in ion strength, which was possibly related to outer-sphere complexes and/or ion exchange. In the presence of fulvic acid (FA), the adsorption of Eu(III) and Am(III) showed high enhancement mainly due to the ternary surface complexes of TPD-FA-Eu(3+) and TPD-FA-Am(3+). The SCM showed that one ion exchange (≡S3Am/Eu) and two ISCs (≡(XO)2Am/EuNO3 and ≡(YO)2Am/EuNO3) seemed more reasonable to quantitatively describe the adsorption edges of both Eu(III) and Am(III). Our findings obviously showed that Eu(III) could be a good analogue to study actinide behaviors in practical terms. However, it should be kept in mind that there are still obvious differences between the characteristics of Eu(III) and Am(III) in some special cases, for instance, the complex ability with organic matter and adsorption affinity to a solid surface. PMID:26198355

  4. Characterization of ribonuclease III from Brucella.

    PubMed

    Wu, Chang-Xian; Xu, Xian-Jin; Zheng, Ke; Liu, Fang; Yang, Xu-Dong; Chen, Chuang-Fu; Chen, Huan-Chun; Liu, Zheng-Fei

    2016-04-01

    Bacterial ribonuclease III (RNase III) is a highly conserved endonuclease, which plays pivotal roles in RNA maturation and decay pathways by cleaving double-stranded structure of RNAs. Here we cloned rncS gene from the genomic DNA of Brucella melitensis, and analyzed the cleavage properties of RNase III from Brucella. We identified Brucella-encoding small RNA (sRNA) by high-throughput sequencing and northern blot, and found that sRNA of Brucella and Homo miRNA precursor (pre-miRNA) can be bound and cleaved by B.melitensis ribonuclease III (Bm-RNase III). Cleavage activity of Bm-RNase III is bivalent metal cations- and alkaline buffer-dependent. We constructed several point mutations in Bm-RNase III, whose cleavage activity indicated that the 133th Glutamic acid residue was required for catalytic activity. Western blot revealed that Bm-RNase III was differently expressed in Brucella virulence strain 027 and vaccine strain M5-90. Collectively, our data suggest that Brucella RNase III can efficiently bind and cleave stem-loop structure of small RNA, and might participate in regulation of virulence in Brucella. PMID:26778206

  5. Mechanisms of Sb(III) Photooxidation by the Excitation of Organic Fe(III) Complexes.

    PubMed

    Kong, Linghao; He, Mengchang

    2016-07-01

    Organic Fe(III) complexes are widely distributed in the aqueous environment, which can efficiently generate free radicals under light illumination, playing a significant role in heavy metal speciation. However, the potential importance of the photooxidation of Sb(III) by organic Fe(III) complexes remains unclear. Therefore, the photooxidation mechanisms of Sb(III) were comprehensively investigated in Fe(III)-oxalate, Fe(III)-citrate and Fe(III)-fulvic acid (FA) solutions by kinetic measurements and modeling. Rapid photooxidation of Sb(III) was observed in an Fe(III)-oxalate solution over the pH range of 3 to 7. The addition of tert-butyl alcohol (TBA) as an ·OH scavenger quenched the Sb(III) oxidation, suggesting that ·OH is an important oxidant for Sb(III). However, the incomplete quenching of Sb(III) oxidation indicated the existence of other oxidants, presumably an Fe(IV) species in irradiated Fe(III)-oxalate solution. In acidic solutions, ·OH may be formed by the reaction of Fe(II)(C2O4) with H2O2, but a hypothetical Fe(IV) species may be generated by the reaction of Fe(II)(C2O4)2(2-) with H2O2 at higher pH. Kinetic modeling provides a quantitative explanation of the results. Evidence for the existence of ·OH and hypothetical Fe(IV) was also observed in an irradiated Fe(III)-citrate and Fe(III)-FA system. This study demonstrated an important pathway of Sb(III) oxidation in surface waters. PMID:27267512

  6. Organoantimony(III)-Containing Tungstoarsenates(III): From Controlled Assembly to Biological Activity.

    PubMed

    Yang, Peng; Bassil, Bassem S; Lin, Zhengguo; Haider, Ali; Alfaro-Espinoza, Gabriela; Ullrich, Matthias S; Silvestru, Cristian; Kortz, Ulrich

    2015-10-26

    A family of three sandwich-type, phenylantimony(III)-containing tungstoarsenates(III), [(PhSb(III) ){Na(H2 O)}As(III) 2 W19 O67 (H2 O)](11-) (1), [(PhSb(III) )2 As(III) 2 W19 O67 (H2 O)](10-) (2), and [(PhSb(III) )3 (B-α-As(III) W9 O33 )2 ](12-) (3), have been synthesized by one-pot procedures and isolated as hydrated alkali metal salts, Cs3 K3.5 Na4.5 [(PhSb(III) ){Na(H2 O)}As(III) 2 W19 O67 (H2 O)]⋅41H2 O (CsKNa-1), Cs4.5 K5.5 [(PhSb(III) )2 As(III) 2 W19 O67 (H2 O)]⋅35H2 O (CsK-2), and Cs4.5 Na7.5 [(PhSb(III) )3 (B-α-As(III) W9 O33 )2 ]⋅42H2 O (CsNa-3). The number of incorporated {PhSb(III) } units could be selectively tuned from one to three by careful control of the reaction parameters. The three compounds were characterized in the solid state by single-crystal XRD, IR spectroscopy, and thermogravimetric analysis. The aqueous solution stability of sandwich polyanions 1-3 was also studied by multinuclear ((1) H, (13) C, (183) W) NMR spectroscopy. Effective inhibitory activity against six different kinds of bacteria was identified for all three polyanions, for which the activity increased with the number of incorporated {PhSb(III) } groups. PMID:26368119

  7. Grant Administration Manual for Title III Coordinators.

    ERIC Educational Resources Information Center

    Mathis, Emily Duncan; Ashmore, Frances W.

    Guidelines for coordinators of programs under Title III of the Higher Education Act of 1965 are presented, based on a national survey of Title III program coordinators. The responsibilities of the coordinator and information on administering the Strengthening Developing Institutions Program (SDIP) grant are covered. The program can either be a…

  8. Preparation of III-V semiconductor nanocrystals

    DOEpatents

    Alivisatos, A.P.; Olshavsky, M.A.

    1996-04-09

    Nanometer-scale crystals of III-V semiconductors are disclosed. They are prepared by reacting a group III metal source with a group V anion source in a liquid phase at elevated temperature in the presence of a crystallite growth terminator such as pyridine or quinoline. 4 figs.

  9. Synthesis, spectroscopic and antimicrobial studies of La(III), Ce(III), Sm(III) and Y(III) Metformin HCl chelates

    NASA Astrophysics Data System (ADS)

    Refat, Moamen S.; Al-Azab, Fathi M.; Al-Maydama, Hussein M. A.; Amin, Ragab R.; Jamil, Yasmin M. S.; Kobeasy, Mohamed I.

    2015-05-01

    Metal complexes of Metformin hydrochloride were prepared using La(III), Ce(III), Sm(III) and Y(III). The resulting complexes were discussed and synthesized to serve as potential insulin-mimetic. Some physical properties and analytical data of the four complexes were checked. The elemental analysis shows that La(III), Ce(III) Sm(III) and Y(III) formed complexes with Metformin in 1:3 (metal:MF) molar ratio. All the synthesized complexes are white and possess high melting points. These complexes are soluble in dimethylsulfoxide and dimethylformamide, partially soluble in hot methanol and insoluble in water and some other organic solvents. From the spectroscopic (infrared, UV-vis and florescence), effective magnetic moment and elemental analyses data, the formula structures are suggested. The results obtained suggested that Metformin reacted with metal ions as a bidentate ligand through its two imino groups. The molar conductance measurements proved that the Metformin complexes are slightly electrolytic in nature. The kinetic thermodynamic parameters such as: E∗, ΔH∗, ΔS∗ and ΔG∗ were estimated from the DTG curves. The antibacterial evaluations of the Metformin and their complexes were also performed against some gram positive, negative bacteria as well as fungi.

  10. The case of King Richard III.

    PubMed

    Skrziepietz, A

    2011-11-01

    In this short essay we will discuss the possible diseases of King Richard III according to the descriptions in Shakespeare's plays King Richard III and Henry VI. Furthermore, it is shown that the description of the defeated enemy as physically and mentally deformed is part of a long tradition which has its roots in Ancient Greece. PMID:22089046

  11. Genes, genetics, and Class III malocclusion.

    PubMed

    Xue, F; Wong, R W K; Rabie, A B M

    2010-05-01

    To present current views that are pertinent to the investigation of the genetic etiology of Class III malocclusion. Class III malocclusion is thought to be a polygenic disorder that results from an interaction between susceptibility genes and environmental factors. However, research on family pedigrees has indicated that Class III malocclusion might also be a monogenic dominant phenotype. Recent studies have reported that genes that encode specific growth factors or other signaling molecules are involved in condylar growth under mechanical strain. These genes, which include Indian hedgehog homolog (IHH), parathyroid-hormone like hormone (PTHLH), insulin-like growth factor-1 (IGF-1), and vascular endothelial growth factor (VEGF), and variations in their levels of expression play an important role in the etiology of Class III malocclusion. In addition, genome-wide scans have revealed chromosomal loci that are associated with Class III malocclusion. It is likely that chromosomal loci 1p36, 12q23, and 12q13 harbor genes that confer susceptibility to Class III malocclusion. In a case-control association study, we identified erythrocyte membrane protein band 4.1 (EPB41) to be a new positional candidate gene that might be involved in susceptibility to mandibular prognathism. Most of the earlier studies on the genetic etiology of Class III malocclusion have focused on the patterns of inheritance of this phenotype. Recent investigations have focused on understanding the genetic variables that affect Class III malocclusion and might provide new approaches to uncovering the genetic etiology of this phenotype. PMID:20477965

  12. Synthesis and in vitro microbial evaluation of La(III), Ce(III), Sm(III) and Y(III) metal complexes of vitamin B6 drug

    NASA Astrophysics Data System (ADS)

    Refat, Moamen S.; Al-Azab, Fathi M.; Al-Maydama, Hussein M. A.; Amin, Ragab R.; Jamil, Yasmin M. S.

    2014-06-01

    Metal complexes of pyridoxine mono hydrochloride (vitamin B6) are prepared using La(III), Ce(III), Sm(III) and Y(III). The resulting complexes are investigated. Some physical properties, conductivity, analytical data and the composition of the four pyridoxine complexes are discussed. The elemental analysis shows that the formed complexes of La(III), Ce(III), Sm(III) and Y(III) with pyridoxine are of 1:2 (metal:PN) molar ratio. All the synthesized complexes are brown in color and possess high melting points. These complexes are partially soluble in hot methanol, dimethylsulfoxide and dimethylformamide and insoluble in water and some other organic solvents. Elemental analysis data, spectroscopic (IR, UV-vis. and florescence), effective magnetic moment in Bohr magnetons and the proton NMR suggest the structures. However, definite particle size is determined by invoking the X-ray powder diffraction and scanning electron microscopy data. The results obtained suggested that pyridoxine reacted with metal ions as a bidentate ligand through its phenolate oxygen and the oxygen of the adjacent group at the 4‧-position. The molar conductance measurements proved that the pyridoxine complexes are electrolytic in nature. The kinetic and thermodynamic parameters such as: Ea, ΔH*, ΔS* and ΔG* were estimated from the DTG curves. The antibacterial evaluation of the pyridoxine and their complexes were also performed against some gram positive, negative bacteria as well as fungi.

  13. Preparation of III-V semiconductor nanocrystals

    DOEpatents

    Alivisatos, A. Paul; Olshavsky, Michael A.

    1996-01-01

    Nanometer-scale crystals of III-V semiconductors are disclosed, They are prepared by reacting a group III metal source with a group V anion source in a liquid phase at elevated temperature in the presence of a crystallite growth terminator such as pyridine or quinoline.

  14. FATE OF CHROMIUM (III) IN CHLORINATED WATER

    EPA Science Inventory

    The oxidation of trivalent chromium, Cr(III), to the more toxic Cr(VI) in chlorinated water is thermodynamically feasible and was the subject of the study. The study found that free available chlorine (FAC) readily converts Cr(III) to Cr(VI) at a rate that is highly dependent upo...

  15. III-V arsenide-nitride semiconductor

    NASA Technical Reports Server (NTRS)

    Major, Jo S. (Inventor); Welch, David F. (Inventor); Scifres, Donald R. (Inventor)

    2000-01-01

    III-V arsenide-nitride semiconductor are disclosed. Group III elements are combined with group V elements, including at least nitrogen and arsenic, in concentrations chosen to lattice match commercially available crystalline substrates. Epitaxial growth of these III-V crystals results in direct bandgap materials, which can be used in applications such as light emitting diodes and lasers. Varying the concentrations of the elements in the III-V materials varies the bandgaps, such that materials emitting light spanning the visible spectra, as well as mid-IR and near-UV emitters, can be created. Conversely, such material can be used to create devices that acquire light and convert the light to electricity, for applications such as full color photodetectors and solar energy collectors. The growth of the III-V material can be accomplished by growing thin layers of elements or compounds in sequences that result in the overall lattice match and bandgap desired.

  16. Title III and toxic torts

    SciTech Connect

    Rodnehausen, G.A.

    1989-07-01

    In July the second annual Toxic Release Inventory (TRI) report under Section 313 of Title III of the Superfund Amendments and Reauthorization Act (SARA), and the computerized, national TRI data-base will be issued. Although the Environmental Protection Agency will not be able to aggregate the July, 1989 reports and issue its own annual report until early next year, we can expect political attention to focus quickly on whether total releases to air, land and water, and in particular total emissions to the air, have increased or decreased from 1987 to 1988. Because the reporting threshold for chemical manufacturing and processing facilities will drop from 75,000 to 50,000 pounds per year, the number of facilities reporting and number of chemicals reported should increase significantly, forcing up total releases. Bringing the totals down will be waste and release reduction efforts already underway in the chemical industry and elsewhere, and perhaps improved accuracy in measurement and estimation of releases. Additions to and deletions from the list of reportable chemicals will also have an effect. Nevertheless, any significant increase in aggregate totals, no matter what the explanation, will be bound to have a political impact on air toxics legislation, and spur public concern with the health risks of air pollution.

  17. Standards in neurosonology. Part III

    PubMed Central

    Tomczyk, Tomasz; Luchowski, Piotr; Kozera, Grzegorz; Kaźmierski, Radosław; Stelmasiak, Zbigniew

    2016-01-01

    The paper presents standards related to ultrasound imaging of the cerebral vasculature and structures. The aim of this paper is to standardize both the performance and description of ultrasound imaging of the extracranial and intracranial cerebral arteries as well as a study of a specific brain structure, i.e. substantia nigra hyperechogenicity. The following aspects are included in the description of standards for each ultrasonographic method: equipment requirements, patient preparation, study technique and documentation as well as the required elements of ultrasound description. Practical criteria for the diagnosis of certain pathologies in accordance with the latest literature were also presented. Furthermore, additional comments were included in some of the sections. Part I discusses standards for the performance, documentation and description of different ultrasound methods (Duplex, Doppler). Part II and III are devoted to standards for specific clinical situations (vasospasm, monitoring after the acute stage of stroke, detection of a right-to-left shunts, confirmation of the arrest of the cerebral circulation, an assessment of the functional efficiency of circle of Willis, an assessment of the cerebrovascular vasomotor reserve as well as the measurement of substantia nigra hyperechogenicity). PMID:27446600

  18. Doublet III Big Dee Project

    SciTech Connect

    Davis, L.G.; Luxon, J.L.

    1985-05-01

    The Doublet III tokamak is presently being reconfigured into a new larger dee-shaped plasma configuration. Experiments will begin in 1986 with a goal of high current, high beta plasma operation at moderate magnetic field. The existing toroidal field coil, Ohmic heating coil, and innermost plasma shaping coils will be retained. A new water-cooled vacuum vessel is being fabricated using a corrugated Inconel sandwich wall construction. Six new water-cooled copper poloidal field coils are also being fabricated. The resultant device along with additional power supplies will provide a capability for plasma currents of 3.5 MA for 1.5 s during the first phase of operations; the tokamak systems are designed for 5 MA operation with additional power systems. The four existing 80 keV, 3 MW neutral beam lines are being modified for optimum torus access and 0.7 s operation. These injectors will be upgraded to allow 5 s operation with new sources in 1987. The device has been designed to accommodate an additional 20 MW of ICRH and ECH power in the future. Limiters and vessel wall protection will be provided for initial operation with up to 40 MJ of input energy. Future installation of additional thermal armor will allow operation with up to 200 MJ of input energy over a 10 s period. Most of the existing diagnostics will be modified as required and reinstalled on the new vessel.

  19. Standards in neurosonology. Part III.

    PubMed

    Wojczal, Joanna; Tomczyk, Tomasz; Luchowski, Piotr; Kozera, Grzegorz; Kaźmierski, Radosław; Stelmasiak, Zbigniew

    2016-06-01

    The paper presents standards related to ultrasound imaging of the cerebral vasculature and structures. The aim of this paper is to standardize both the performance and description of ultrasound imaging of the extracranial and intracranial cerebral arteries as well as a study of a specific brain structure, i.e. substantia nigra hyperechogenicity. The following aspects are included in the description of standards for each ultrasonographic method: equipment requirements, patient preparation, study technique and documentation as well as the required elements of ultrasound description. Practical criteria for the diagnosis of certain pathologies in accordance with the latest literature were also presented. Furthermore, additional comments were included in some of the sections. Part I discusses standards for the performance, documentation and description of different ultrasound methods (Duplex, Doppler). Part II and III are devoted to standards for specific clinical situations (vasospasm, monitoring after the acute stage of stroke, detection of a right-to-left shunts, confirmation of the arrest of the cerebral circulation, an assessment of the functional efficiency of circle of Willis, an assessment of the cerebrovascular vasomotor reserve as well as the measurement of substantia nigra hyperechogenicity). PMID:27446600

  20. Large Hexadecametallic {Mn(III) -Ln(III) } Wheels: Synthesis, Structural, Magnetic, and Theoretical Characterization.

    PubMed

    Vignesh, Kuduva R; Langley, Stuart K; Moubaraki, Boujemaa; Murray, Keith S; Rajaraman, Gopalan

    2015-11-01

    The synthesis, gas sorption studies, magnetic properties, and theoretical studies of new molecular wheels of core type {Mn(III) 8 Ln(III) 8 } (Ln=Dy, Ho, Er, Y and Yb), using the ligand mdeaH2 , in the presence of ortho-toluic or benzoic acid are reported. From the seven wheels studied the {Mn8 Dy8 } and {Mn8 Y8 } analogues exhibit SMM behavior as determined from ac susceptibility experiments in a zero static magnetic field. From DFT calculations a S=16 ground state was determined for the {Mn8 Y8 } complex due to weak ferromagnetic Mn(III) -Mn(III) interactions. Ab initio CASSCF+RASSI-SO calculations on the {Mn8 Dy8 } wheel estimated the Mn(III) -Dy(III) exchange interaction as -0.1 cm(-1) . This weak exchange along with unfavorable single-ion anisotropy of Dy(III) /Mn(III) ions, however, led to the observation of SMM behavior with fast magnetic relaxation. The orientation of the g-anisotropy of the Dy(III) ions is found to be perpendicular to the plane of the wheel and this suggests the possibility of toroidal magnetic moments in the cluster. The {Mn8 Ln8 } clusters reported here are the largest heterometallic Mn(III) Ln(III) wheels and the largest {3d-4f} wheels to exhibit SMM behavior reported to date. PMID:26403264

  1. Mechanisms of Eu(III) and Cm(III) Association With Chlorella Vulgaris

    NASA Astrophysics Data System (ADS)

    Ozaki, T.; Kimura, T.; Ohnuki, T.; Francis, A. J.

    2002-12-01

    Association of Eu(III) and Cm(III) with Chlorella vulgaris and cellulose was studied by a batch method, time-resolved laser-induced fluorescence spectroscopy (TRLFS) and extended X-ray absorption fine structure (EXAFS). The kinetics study performed by a batch method showed that the maximum adsorption of Eu(III) and Cm(III) on C. vulgaris was attained within three minutes of contact time, and afterwards the percentage adsorption decreased with time due to exudates released from C. vulgaris with affinity for Eu(III) and Cm(III). TRLFS showed that the short-term adsorption of Eu(III) on C. vulgaris was attributed to their coordination with the cell wall components comprised of cellulose. TRLFS also demonstrated that Eu(III) coordinated with the functional groups of cellulose very weakly in spite of the large distribution coefficients observed. EXAFS analysis showed the local structure around the Eu(III) adsorbed on cellulose and with C. vulgaris was similar. These results indicate that the reactions both at cell surfaces through the adsorption as well as in solution phases through chelation with the exudates are important in estimating the environmental behavior of Eu(III) and Cm(III) in aqueous environments.

  2. Separation studies of As(III), Sb(III) and Bi(III) by reversed-phase paper chromatographic technique

    SciTech Connect

    Raman, B.; Shinde, V.M.

    1987-07-01

    Reversed-phase paper chromatographic separations of As(III), Sb(III) and Bi(III) have been carried out on Whatman No 1 filter paper impregnated with triphenylphosphine oxide as stationary phase and using organic complexing agents such as sodium acetate, sodium succinate and sodium malonate solutions as active mobile phases. Results for the separation of binary and ternary mixtures are reported and the method has been successfully applied to the separation and detection of these elements present in real samples and at ppm level concentration.

  3. Rapid photooxidation of Sb(III) in the presence of different Fe(III) species

    NASA Astrophysics Data System (ADS)

    Kong, Linghao; He, Mengchang; Hu, Xingyun

    2016-05-01

    The toxicity and mobility of antimony (Sb) are strongly influenced by the redox processes associated with Sb. Dissolved iron (Fe) is widely distributed in the environment as different species and plays a significant role in Sb speciation. However, the mechanisms of Sb(III) oxidation in the presence of Fe have remained unclear because of the complexity of Fe and Sb speciation. In this study, the mechanisms of Sb(III) photooxidation in the presence of different Fe species were investigated systematically. The photooxidation of Sb(III) occurred over a wide pH range, from 1 to 10. Oxygen was not a predominant or crucial factor in the Sb(III) oxidation process. The mechanism of Sb(III) photooxidation varied depending on the Fe(III) species. In acidic solution (pH 1-3), dichloro radicals (radCl2-) and hydroxyl radicals (radOH) generated by the photocatalysis of FeCl2+ and FeOH2+ were the main oxidants for Sb(III) oxidation. Fe(III) gradually transformed into the colloid ferric hydroxide (CFH) and ferrihydrite in circumneutral and alkaline solutions (pH 4-10). Photooxidation of Sb(III) occurred through electron transfer from Sb(III) to Fe(III) along with the reduction of Fe(III) to Fe(II) through a ligand-to-metal charge-transfer (LMCT) process. The photocatalysis of different Fe(III) species may play an important role in the geochemical cycle of Sb(III) in surface soil and aquatic environments.

  4. Neptunium(III) application in extraction chromatography.

    PubMed

    Guérin, Nicolas; Nadeau, Kenny; Larivière, Dominic

    2011-12-15

    This paper describes a novel strategy for actinide separation by extraction chromatography with Np(III) valence adjustment. Neptunium(IV) was reduced to Np(III) using Cr(II) and then selectively separated from uranium (IV) on a TEVA resin. After elution, Np(III) was retained on a DGA resin in order to remove any detrimental chromium impurities. Neptunium(III) formation was demonstrated by the complete and selective elution of Np from TEVA resin (99 ± 7%) in less than 12 mL of 9M HCl from U(IV) (0.7 ± 0.7%). It was determined by UV-visible and kinetic studies that Cr(II) was the only species responsible for the elution of Np(IV) as Np(III) and that the Cr(II) solution could be prepared from 2 to 30 min before its use without the need of complex degassing systems to prevent the oxidation of Np(III) by oxygen. The methodology proposed here with TEVA/DGA resins provides removal of Cr(III) impurities produced at high decontamination factors (2.8 × 10(3) and 7.3 × 10(4) respectively). PMID:22099641

  5. Timely management of developing class III malocclusion.

    PubMed

    Yelampalli, M R; Rachala, M R

    2012-01-01

    Timing of orthodontic treatment, especially for children with developing class III malocclusions, has always been somewhat controversial, and definitive treatment tends to be delayed for severe class III cases. Developing class III patients with moderate to severe anterior crossbite and deep bite may need early intervention in some selected cases. Class III malocclusion may develop in children as a result of an inherent growth abnormality, i.e. true class III malocclusion, or as a result of premature occlusal contacts causing forward functional shift of the mandible, which is known as pseudo class III malocclusion. These cases, if not treated at the initial stage of development, interfere with normal growth of the jaw bases and may result in severe facial deformities. The treatment should be carried out as early as possible for permitting normal growth of the skeletal bases. This paper deals with the selection of an appropriate appliance from the various current options available for early intervention in developing class III malocclusion through two case reports. PMID:22565523

  6. Photodetectors using III-V nitrides

    DOEpatents

    Moustakas, Theodore D.; Misra, Mira

    1997-01-01

    A photodetector using a III-V nitride and having predetermined electrical properties is disclosed. The photodetector includes a substrate with interdigitated electrodes formed on its surface. The substrate has a sapphire base layer, a buffer layer formed from a III-V nitride and a single crystal III-V nitride film. The three layers are formed by electron cyclotron resonance microwave plasma-assisted molecular beam epitaxy (ECR-assisted MBE). Use of the ECR-assisted MBE process allows control and predetermination of the electrical properties of the photodetector.

  7. Photodetectors using III-V nitrides

    DOEpatents

    Moustakas, T.D.; Misra, M.

    1997-10-14

    A photodetector using a III-V nitride and having predetermined electrical properties is disclosed. The photodetector includes a substrate with interdigitated electrodes formed on its surface. The substrate has a sapphire base layer, a buffer layer formed from a III-V nitride and a single crystal III-V nitride film. The three layers are formed by electron cyclotron resonance microwave plasma-assisted molecular beam epitaxy (ECR-assisted MBE). Use of the ECR-assisted MBE process allows control and predetermination of the electrical properties of the photodetector. 24 figs.

  8. Genetics Home Reference: mucolipidosis III alpha/beta

    MedlinePlus

    ... Health Conditions mucolipidosis III alpha/beta mucolipidosis III alpha/beta Enable Javascript to view the expand/collapse ... PDF Open All Close All Description Mucolipidosis III alpha/beta is a slowly progressive disorder that affects ...

  9. Complexation of N4-Tetradentate Ligands with Nd(III) and Am(III)

    SciTech Connect

    Ogden, Mark D.; Sinkov, Sergey I.; Meier, G. Patrick; Lumetta, Gregg J.; Nash, Kenneth L.

    2012-12-06

    To improve understanding of aza-complexants in trivalent actinide–lanthanide separations, a series of tetradentate N-donor ligands have been synthesized and their complexation of americium(III) and neodymium(III) investigated by UV–visible spectrophotometry in methanolic solutions. The six pyridine/alkyl amine/imine ligands are N,N0-bis(2-methylpyridyl)-1,2-diaminoethane, N,N0-bis(2-methylpyridyl)-1,3-diaminopropane, trans-N,N-bis(2-pyridylmethyl)-1,2-diaminocyclohexane (BPMDAC), N,N’-bis(2-pyridylmethyl)piperazine, N,N’-bis-[pyridin-2-ylmethylene]ethane-1,2-diamine, and trans-N,Nbis-([pyridin-2-ylmethylene]-cyclohexane-1,2-diamine. Each ligand has two pyridine groups and two aliphatic amine/imine N-donor atoms arranged with different degrees of preorganization and structural backbone rigidity. Conditional stability constants for the complexes of Am(III) and Nd(III) by these ligands establish the selectivity patterns. The overall selectivity of Am(III) over Nd(III) is similar to that reported for the terdentate bis(dialkyltriazinyl)pyridine molecules. The cyclohexane amine derivative (BPMDAC) is the strongest complexant and shows the highest selectivity for Am(III) over Nd(III) while the imines appear to prefer a bridging arrangement between two cations. These results suggest that this series of ligands could be employed to develop an enhanced actinide(III)– lanthanide(III) separation system.

  10. Extraction chromatographic separation of Am(III) and Eu(III) by TPEN-immobilized gel

    SciTech Connect

    Takeshita, K.; Ogata, T.; Oaki, H.; Inaba, Y.; Mori, A.; Yaita, T.; Koyama, S.I.

    2013-07-01

    A TPEN derivative with 4 vinyl groups, N,N,N',N' -tetrakis-(4-propenyloxy-2-pyridylmethyl)ethylenediamine (TPPEN) was synthesized for the separation of trivalent minor actinides (Am(III)) and lanthanides (Eu(III)). A co-polymer gel with TPPEN and N-isopropylacrylamide (NIPA) showed a high separation factor of Am(III) over Eu(III) (SF[Am/Eu]), which was evaluated to be 26 at pH=5. Thin film of NIPA-TPPEN gel (average thickness: 2-40 nm) was immobilized on the pore surface in porous silica particles (particle diameter : 50 μm, average pore diameter : 50 and 300 nm) and a chromatographic column (diameter: 6 mm, height: 11 mm) packed with the gel-coated particles was prepared. A small amount of weakly acidic solution (pH=4) containing Am(III) and Eu(III) was supplied in the column and the elution tests of Am(III) and Eu(III) were carried out. Eu(III) was recovered separately by a weakly acidic eluent (pH=4) at 313 K and Am(III) by a highly acidic eluent (pH=2) at 298 K. These results suggest that the contentious separation of minor actinides and lanthanides is attainable by a new extraction chromatographic process with two columns adjusted to 298 K and 313 K. (authors)

  11. Potentiometry: A Chromium (III) -- EDTA Complex

    ERIC Educational Resources Information Center

    Hoppe, J. I.; Howell, P. J.

    1975-01-01

    Describes an experiment that involves the preparation of a chromium (III)-EDTA compound, a study of its infrared spectrum, and the potentiometric determination of two successive acid dissociation constants. (Author/GS)

  12. Synthesis, thermal and spectroscopic behaviors of metal-drug complexes: La(III), Ce(III), Sm(III) and Y(III) amoxicillin trihydrate antibiotic drug complexes

    NASA Astrophysics Data System (ADS)

    Refat, Moamen S.; Al-Maydama, Hussein M. A.; Al-Azab, Fathi M.; Amin, Ragab R.; Jamil, Yasmin M. S.

    2014-07-01

    The metal complexes of Amoxicillin trihydrate with La(III), Ce(III), Sm(III) and Y(III) are synthesized with 1:1 (metal:Amox) molar ratio. The suggested formula structures of the complexes are based on the results of the elemental analyses, molar conductivity, (infrared, UV-visible and fluorescence) spectra, effective magnetic moment in Bohr magnetons, as well as the thermal analysis (TG), and characterized by X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The results obtained suggested that Amoxicillin reacted with metal ions as tridentate ligands, coordinating the metal ion through its amino, imino, and β-lactamic carbonyl. The kinetic thermodynamic parameters such as: Ea, ΔH*, ΔS* and ΔG* were estimated from the DTG curves.

  13. SAGE III/Meteor - 3M

    NASA Technical Reports Server (NTRS)

    1999-01-01

    Full view of the SAGE III Bench Checkout Unit, Collimated Source Bench (CSB), Portable Image Generator (PIG) on tripod, and Stratospheric Aerosol Gastropheric Experiment (SAGE)/Meteor - 3M flight instrument. Photographed in building 1250, 40 foot clean room.

  14. SAGE III/Meteor - 3M

    NASA Technical Reports Server (NTRS)

    1999-01-01

    Back view of the SAGE III Bench Checkout Unit, Portable Image Generator (PIG) on tripod, and the Stratospheric Aerosol Gastropheric Experiment (SAGE)/Meteor - 3M flight instrument. Photographed in building 1250, 40 foot clean room.

  15. Sorption of indium (III) onto carbon nanotubes.

    PubMed

    Alguacil, F J; Lopez, F A; Rodriguez, O; Martinez-Ramirez, S; Garcia-Diaz, I

    2016-08-01

    Indium has numerous applications in different industrial sectors and is not an abundant element. Therefore appropriate technology to recover this element from various process wastes is needed. This research reports high adsorption capacity of multiwalled carbon nanotubes (MWCNT) for In(III). The effects of pH, kinetics, isotherms and adsorption mechanism of MWCNT on In(III) adsorption were investigated and discussed in detail. The pH increases improves the adsorption capacity for In(III). The Langmuir adsorption model is the best fit with the experimental data. For the kinetic study, the adsorption onto MWCNT could be fitted to pseudo second-order. The adsorption of indium(III) can be described to a mechanism which consists of a film diffusion controlled process. Metal desorption can be achieved with acidic solutions. PMID:27085001

  16. SAGE III capabilities and global change

    NASA Technical Reports Server (NTRS)

    Mccormick, M. Patrick

    1991-01-01

    The science objectives of the satellite-borne SAGE III are presented as they pertain to detecting global change. SAGE III is the proposed follow on and improved version of SAM II, SAGE I and SAGE II which have measured stratospheric and, in some cases, tropospheric species since late 1978. Specifically, SAGE III will measure profiles of aerosols, ozone, water vapor, nitrogen dioxide and trioxide, neutral density, temperature, clouds, and chlorine dioxide using the solar and lunar occultation techniques. These techniques are inherently self-calibrating, provide high vertical resolution, and use well-behaved data retrievals making them ideal for trend detection and global change studies. The potential capabilities of SAGE III are illustrated by using data and results from SAM II, SAGE I and SAGE II.

  17. SAGE III solar ozone measurements: Initial results

    NASA Technical Reports Server (NTRS)

    Wang, Hsiang-Jui; Cunnold, Derek M.; Trepte, Chip; Thomason, Larry W.; Zawodny, Joseph M.

    2006-01-01

    Results from two retrieval algorithms, o3-aer and o3-mlr , used for SAGE III solar occultation ozone measurements in the stratosphere and upper troposphere are compared. The main differences between these two retrieved (version 3.0) ozone are found at altitudes above 40 km and below 15 km. Compared to correlative measurements, the SAGE II type ozone retrievals (o3-aer) provide better precisions above 40 km and do not induce artificial hemispheric differences in upper stratospheric ozone. The multiple linear regression technique (o3_mlr), however, can yield slightly more accurate ozone (by a few percent) in the lower stratosphere and upper troposphere. By using SAGE III (version 3.0) ozone from both algorithms and in their preferred regions, the agreement between SAGE III and correlative measurements is shown to be approx.5% down to 17 km. Below 17 km SAGE III ozone values are systematically higher, by 10% at 13 km, and a small hemispheric difference (a few percent) appears. Compared to SAGE III and HALOE, SAGE II ozone has the best accuracy in the lowest few kilometers of the stratosphere. Estimated precision in SAGE III ozone is about 5% or better between 20 and 40 km and approx.10% at 50 km. The precision below 20 km is difficult to evaluate because of limited coincidences between SAGE III and sondes. SAGE III ozone values are systematically slightly larger (2-3%) than those from SAGE II but the profile shapes are remarkably similar for altitudes above 15 km. There is no evidence of any relative drift or time dependent differences between these two instruments for altitudes above 15-20 km.

  18. The Mark III Hypercube-Ensemble Computers

    NASA Technical Reports Server (NTRS)

    Peterson, John C.; Tuazon, Jesus O.; Lieberman, Don; Pniel, Moshe

    1988-01-01

    Mark III Hypercube concept applied in development of series of increasingly powerful computers. Processor of each node of Mark III Hypercube ensemble is specialized computer containing three subprocessors and shared main memory. Solves problem quickly by simultaneously processing part of problem at each such node and passing combined results to host computer. Disciplines benefitting from speed and memory capacity include astrophysics, geophysics, chemistry, weather, high-energy physics, applied mechanics, image processing, oil exploration, aircraft design, and microcircuit design.

  19. Population III Stars Around the Milky Way

    NASA Astrophysics Data System (ADS)

    Komiya, Yutaka; Suda, Takuma; Fujimoto, Masayuki Y.

    2016-03-01

    We explore the possibility of observing Population III (Pop III) stars, born of primordial gas. Pop III stars with masses below 0.8 M⊙ should survive to date though are not yet observed, but the existence of stars with low metallicity as [{{Fe}}/{{H}}]\\lt -5 in the Milky Way halo suggests the surface pollution of Pop III stars with accreted metals from the interstellar gas after birth. In this paper, we investigate the runaway of Pop III stars from their host mini-halos, considering the ejection of secondary members from binary systems when their massive primaries explode as supernovae. These stars save them from surface pollution. By computing the star formation and chemical evolution along with the hierarchical structure formation based on the extended Press-Schechter merger trees, we demonstrate that several hundreds to tens of thousands of low-mass Pop III stars escape from the building blocks of the Milky Way. The second and later generations of extremely metal-poor stars also escaped from the mini-halos. We discuss the spatial distributions of these escaped stars by evaluating the distances between the mini-halos in the branches of merger trees under the spherical collapse model of dark matter halos. It is demonstrated that the escaped stars distribute beyond the stellar halo with a density profile close to the dark matter halo, while Pop III stars are slightly more centrally concentrated. 6%-30% of the escaped stars leave the Milky Way and go out into the intergalactic space. Based on the results, we discuss the feasibility of observing the Pop III stars with the pristine surface abundance.

  20. Lanthanum(III) and praseodymium(III) derivatives with dithiocarbamates derived from α-amino acids

    NASA Astrophysics Data System (ADS)

    Rai, Anita; Sengupta, Soumitra K.; Pandey, Om P.

    2006-06-01

    Lanthanum(III) and praseodymium(III) complexes with dithiocarbamates have been synthesized by the reactions of lanthanum(III) and praseodymium(III) chloride with barium dithiocarbamate and complexes of type [LnCl(L)H 2O] n have been obtained (where Ln = La(III) or Pr(III); L = barium salt of dithiocarbamate derived from glycine, L-leucine, L-valine, DL-alanine). The complexes have been characterized by elemental analysis, molar conductance, electronic absorption and fluorescence, infrared, far infrared, 1H NMR spectral studies. The presence of coordinated water molecule is inferred from thermogravimetric analysis which indicates the loss of one water molecule at 150-170 °C. The oscillator strength, Judd-Ofelt intensity parameter, stimulated emission cross-section, etc. have been obtained for different transitions of Pr 3+.

  1. Gallium(iii) and iron(iii) complexes of quinolone antimicrobials.

    PubMed

    Mjos, Katja Dralle; Cawthray, Jacqueline F; Polishchuk, Elena; Abrams, Michael J; Orvig, Chris

    2016-08-16

    Iron is an essential nutrient for many microbes. According to the "Trojan Horse Hypothesis", biological systems have difficulties distinguishing between Fe(3+) and Ga(3+), which constitutes the antimicrobial efficacy of the gallium(iii) ion. Nine novel tris(quinolono)gallium(iii) complexes and their corresponding iron(iii) analogs have been synthesized and fully characterized. Quinolone antimicrobial agents from three drug generations were used in this study: ciprofloxacin, enoxacin, fleroxacin, levofloxacin, lomefloxacin, nalidixic acid, norfloxacin, oxolinic acid, and pipemidic acid. The antimicrobial efficacy of the tris(quinolono)gallium(iii) complexes was studied against E. faecalis and S. aureus (both Gram-positive), as well as E. coli, K. pneumonia, and P. aeruginosa (all Gram-negative) in direct comparison to the tris(quinolono)iron(iii) complexes and the corresponding free quinolone ligands at various concentrations. For the tris(quinolono)gallium(iii) complexes, no combinational antimicrobial effects between Ga(3+) and the quinolone antimicrobial agents were observed. PMID:27315225

  2. Deficiencies of Natural Anticoagulants, Protein C, Protein S, and Antithrombin

    MedlinePlus

    ... V Leiden . Circulation . 2003 ; 107 : e94 – e97 FREE Full Text 2. ↵ Varga EA , Moll S . Prothrombin 20210 mutation (factor II mutation) . Circulation . 2004 ; 110 : e15 – e18 FREE Full Text CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon ...

  3. Design of Integrated III-Nitride/Non-III-Nitride Tandem Photovoltaic Devices

    SciTech Connect

    Toledo, N. G.; Friedman, D.J.; Farrell, R. M.; Perl, E. E.; Lin, C. T.; Bowers, J. E.; Speck, J. S.; Mishra, U. K.

    2012-03-01

    The integration of III-nitride and non-III-nitride materials for tandem solar cell applications can improve the efficiency of the photovoltaic device due to the added power contributed by the III-nitride top cell to that of high-efficiency multi-junction non-III-nitride solar cells if the device components are properly designed and optimized. The proposed tandem solar cell is comprised of a III-nitride top cell bonded to a non-III-nitride, series-constrained, multi-junction subcell. The top cell is electrically isolated, but optically coupled to the underlying subcell. The use of a III-nitride top cell is potentially beneficial when the top junction of a stand-alone non-III-nitride subcell generates more photocurrent than the limiting current of the non-III-nitride subcell. Light producing this excess current can either be redirected to the III-nitride top cell through high energy photon absorption, redirected to the lower junctions through layer thickness optimization, or a combination of both, resulting in improved total efficiency. When the non-III-nitride cell's top junction is the limiting junction, the minimum power conversion efficiency that the III-nitride top cell must contribute should compensate for the spectrum filtered from the multi-junction subcell for this design to be useful. As the III-nitride absorption edge wavelength, {lambda}{sub N}, increases, the performance of the multi-junction subcell decreases due to spectral filtering. In the most common spectra of interest (AM1.5G, AM1.5 D, and AM0), the technology to grow InGaN cells with {lambda}{sub N}<520 nm is found to be sufficient for III-nitride top cell applications. The external quantum efficiency performance, however, of state-of-the-art InGaN solar cells still needs to be improved. The effects of surface/interface reflections are also presented. The management of these reflection issues determines the feasibility of the integrated III-nitride/non-III-nitride design to improve overall cell

  4. Association of Eu(III) and Cm(III) With Halophiles

    NASA Astrophysics Data System (ADS)

    Ozaki, T.; Takenaka, Y.; Ohnuki, T.; Gillow, J. B.; Francis, A. J.

    2003-12-01

    Halophiles live in high ionic strength brine. The mechanisms of metal association with these microorganisms are poorly understood. In this study, we determined the distribution of Eu(III) and Cm(III) on halophiles, Halomonas sp. (WIPP1A) which was isolated from the Waste Isolation Pilot Plant (WIPP) repository in Carlsbad, US., Halomonas elongata (ATCC33173), Halobacterium salinarum (ATCC19700), and Halobacterium halobium (ATCC43214) and examined the coordination environment of Eu(III) adsorbed on the cells by time-resolved laser-induced fluorescence spectroscopy (TRLFS). The cells of Halomonas sp. and H. elongata were grown in media containing 10 - 15 w/v% and 3.5 - 30 w/v% NaCl, respectively. Halobacterium salinarum and H. halobium were grown in media containing 25 w/v% NaCl. The logarithmic distribution coefficient (log Kd) was measured by using the cells at the late exponential phase. After washing the cells with the same concentrations of NaCl, the cells were mixed with 1x10-6 mol dm-3 Eu(III) and 1x10-8 mol dm-3 Cm(III) at pH 5 in the same concentrations of NaCl and log Kd of Eu(III) and Cm(III) was determined. For Halomonas sp. and H. elongata, log Kd was determined as a function of NaCl concentrations. The coordination environment of Eu(III) adsorbed on the cells was estimated by TRLFS. For TRLFS measurements, samples were prepared by adding cells to a solution of 1x10-3 mol dm-3 Eu(III) with the same concentrations of NaCl as the culture media. For Halomonas sp. and H. elongata, log Kd of Cm(III) was apparently larger than that of Eu(III) at all the NaCl concentrations examined. On the other hand, log Kd of Eu(III) and Cm(III) for H. salinarum and H. halobium was almost identical. Our previous study demonstrated that non-halophiles, Chlorella vulgaris, Bacillus subtilis, and Pseudomonas fluorescens show no preferences between these elements. Chemical properties of Eu(III) and Cm(III) are almost identical. Our findings suggest that the difference in log Kd

  5. Sparkle/PM3 Parameters for the Modeling of Neodymium(III), Promethium(III), and Samarium(III) Complexes.

    PubMed

    Freire, Ricardo O; da Costa, Nivan B; Rocha, Gerd B; Simas, Alfredo M

    2007-07-01

    The Sparkle/PM3 model is extended to neodymium(III), promethium(III), and samarium(III) complexes. The unsigned mean error, for all Sparkle/PM3 interatomic distances between the trivalent lanthanide ion and the ligand atoms of the first sphere of coordination, is 0.074 Å for Nd(III); 0.057 Å for Pm(III); and 0.075 Å for Sm(III). These figures are similar to the Sparkle/AM1 ones of 0.076 Å, 0.059 Å, and 0.075 Å, respectively, indicating they are all comparable models. Moreover, their accuracy is similar to what can be obtained by present-day ab initio effective potential calculations on such lanthanide complexes. Hence, the choice of which model to utilize will depend on the assessment of the effect of either AM1 or PM3 on the quantum chemical description of the organic ligands. Finally, we present a preliminary attempt to verify the geometry prediction consistency of Sparkle/PM3. Since lanthanide complexes are usually flexible, we randomly generated 200 different input geometries for the samarium complex QIPQOV which were then fully optimized by Sparkle/PM3. A trend appeared in that, on average, the lower the total energy of the local minima found, the lower the unsigned mean errors, and the higher the accuracy of the model. These preliminary results do indicate that attempting to find, with Sparkle/PM3, a global minimum for the geometry of a given complex, with the understanding that it will tend to be closer to the experimental geometry, appears to be warranted. Therefore, the sparkle model is seemingly a trustworthy semiempirical quantum chemical model for the prediction of lanthanide complexes geometries. PMID:26633229

  6. The Berkeley SETI Program: SERENDIP III

    NASA Astrophysics Data System (ADS)

    Donnelly, C.; Bowyer, S.; Werthimer, D.; Ng, D.; Cobb, J.

    1993-05-01

    The Berkeley SETI (Search for Extraterrestrial Intelligence) program, named SERENDIP, was begun in the late 1970's. It is aimed at detecting narrow band radio signals from extraterrestrial civilizations. The SERENDIP I, II and III systems have operated autonomously in a piggyback search mode, conducting unobtrusive, long-term observations on the world's largest radio telescopes. The latest generation SERENDIP instrument, SERENDIP III, is a four million channel FFT-based spectrum analyzer operating at 0.6 Hz resolution with a 1.7 second integration time. SERENDIP III has been operating at the NAIC Arecibo Observatory since April, 1992. Several independent criteria suggest that this search is the most sensitive SETI search in operation. To date SERENDIP III has accumulated over 3600 hours of high quality telescope time, observing 75% of the sky visible by the Arecibo telescope. Over this period SERENDIP III has analyzed over 30 trillion spectral bins, and recorded information on 110 million strong narrow band signals in the 424--435 MHz band. A handful of these signals have survived our RFI rejection and signal detection algorithms, and have thus been added to our list of ETI candidate signals. A follow-up observation program will be conducted next year in an attempt to verify each of these candidate signals. This work has been supported by NASA grant NAGW-2722.

  7. Stoichiometries of arsenazo III-Ca complexes.

    PubMed Central

    Palade, P; Vergara, J

    1983-01-01

    The equilibrium interactions of the metallochromic indicator arsenazo III with calcium at physiological ionic strength and pH were investigated spectrophotometrically and with the aid of a calcium electrode. Evidence suggests the formation of more than one dye-calcium complex. The analysis of data obtained over a 10,000-fold range of dye concentrations concludes that at the concentrations used for in vitro biochemical studies (10--100 microM), arsenazo III absorbance changes in response to calcium binding primarily involve the formation of a complex involving two dye molecules and two calcium ions. At millimolar dye concentrations, typical of physiological calcium transient determinations in situ, a second complex involving two arsenazo III molecules and one calcium ion is additionally formed. A third complex, involving one arsenazo III molecule and one calcium ion, is formed at very low dye concentrations. The results reported here suggest that equilibrium calibration of the dye with calcium cannot be used directly to satisfactorily relate transient absorbance changes in physiological preparations to calcium concentration changes since several stoichiometrically distinct complexes with different absorbances could be formed at different rates. The results of this study do not permit the elucidation of a unique kinetic scheme of arsenazo III complexation with calcium; for this, in vitro kinetic analysis is required. Results of similar analysis of the dye interaction with magnesium are also reported, and these appear compatible with a much simpler model of complexation. PMID:6626673

  8. A semisimultaneous inversion algorithm for SAGE III

    NASA Astrophysics Data System (ADS)

    Ward, Dale M.

    2002-12-01

    The Stratospheric Aerosol and Gas Experiment (SAGE) III instrument was successfully launched into orbit on 10 December 2001. The planned operational species separation inversion algorithm will utilize a stepwise retrieval strategy. This paper presents an alternative, semisimultaneous species separation inversion that simultaneously retrieves all species over user-specified vertical intervals or blocks. By overlapping these vertical blocks, retrieved species profiles over the entire vertical range of the measurements are obtained. The semisimultaneous retrieval approach provides a more straightforward method for evaluating the error coupling that occurs among the retrieved profiles due to various types of input uncertainty. Simulation results are presented to show how the semisimultaneous inversion can enhance understanding of the SAGE III retrieval process. In the future, the semisimultaneous inversion algorithm will be used to help evaluate the results and performance of the operational inversion. Compared to SAGE II, SAGE III will provide expanded and more precise spectral measurements. This alone is shown to significantly reduce the uncertainties in the retrieved ozone, nitrogen dioxide, and aerosol extinction profiles for SAGE III. Additionally, the well-documented concern that SAGE II retrievals are biased by the level of volcanic aerosol is greatly alleviated for SAGE III.

  9. Hybrid III-V/silicon lasers

    NASA Astrophysics Data System (ADS)

    Kaspar, P.; Jany, C.; Le Liepvre, A.; Accard, A.; Lamponi, M.; Make, D.; Levaufre, G.; Girard, N.; Lelarge, F.; Shen, A.; Charbonnier, P.; Mallecot, F.; Duan, G.-H.; Gentner, J.-.; Fedeli, J.-M.; Olivier, S.; Descos, A.; Ben Bakir, B.; Messaoudene, S.; Bordel, D.; Malhouitre, S.; Kopp, C.; Menezo, S.

    2014-05-01

    The lack of potent integrated light emitters is one of the bottlenecks that have so far hindered the silicon photonics platform from revolutionizing the communication market. Photonic circuits with integrated light sources have the potential to address a wide range of applications from short-distance data communication to long-haul optical transmission. Notably, the integration of lasers would allow saving large assembly costs and reduce the footprint of optoelectronic products by combining photonic and microelectronic functionalities on a single chip. Since silicon and germanium-based sources are still in their infancy, hybrid approaches using III-V semiconductor materials are currently pursued by several research laboratories in academia as well as in industry. In this paper we review recent developments of hybrid III-V/silicon lasers and discuss the advantages and drawbacks of several integration schemes. The integration approach followed in our laboratory makes use of wafer-bonded III-V material on structured silicon-on-insulator substrates and is based on adiabatic mode transfers between silicon and III-V waveguides. We will highlight some of the most interesting results from devices such as wavelength-tunable lasers and AWG lasers. The good performance demonstrates that an efficient mode transfer can be achieved between III-V and silicon waveguides and encourages further research efforts in this direction.

  10. Failures in Phase III: Causes and Consequences.

    PubMed

    Seruga, Bostjan; Ocana, Alberto; Amir, Eitan; Tannock, Ian F

    2015-10-15

    Phase III randomized controlled trials (RCT) in oncology fail to lead to registration of new therapies more often than RCTs in other medical disciplines. Most RCTs are sponsored by the pharmaceutical industry, which reflects industry's increasing responsibility in cancer drug development. Many preclinical models are unreliable for evaluation of new anticancer agents, and stronger evidence of biologic effect should be required before a new agent enters the clinical development pathway. Whenever possible, early-phase clinical trials should include pharmacodynamic studies to demonstrate that new agents inhibit their molecular targets and demonstrate substantial antitumor activity at tolerated doses in an enriched population of patients. Here, we review recent RCTs and found that these conditions were not met for most of the targeted anticancer agents, which failed in recent RCTs. Many recent phase III RCTs were initiated without sufficient evidence of activity from early-phase clinical trials. Because patients treated within such trials can be harmed, they should not be undertaken. The bar should also be raised when making decisions to proceed from phase II to III and from phase III to marketing approval. Many approved agents showed only better progression-free survival than standard treatment in phase III trials and were not shown to improve survival or its quality. Introduction of value-based pricing of new anticancer agents would dissuade the continued development of agents with borderline activity in early-phase clinical trials. When collaborating with industry, oncologists should be more critical and better advocates for cancer patients. PMID:26473191

  11. 46 CFR 50.30-20 - Class III pressure vessels.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Class III pressure vessels. 50.30-20 Section 50.30-20... Fabrication Inspection § 50.30-20 Class III pressure vessels. (a) Class III pressure vessels shall be subject... specifically exempted by other regulations in this subchapter. (b) For Class III welded pressure vessels,...

  12. 46 CFR 50.30-20 - Class III pressure vessels.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Class III pressure vessels. 50.30-20 Section 50.30-20... Fabrication Inspection § 50.30-20 Class III pressure vessels. (a) Class III pressure vessels shall be subject... specifically exempted by other regulations in this subchapter. (b) For Class III welded pressure vessels,...

  13. 46 CFR 50.30-20 - Class III pressure vessels.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Class III pressure vessels. 50.30-20 Section 50.30-20... Fabrication Inspection § 50.30-20 Class III pressure vessels. (a) Class III pressure vessels shall be subject... specifically exempted by other regulations in this subchapter. (b) For Class III welded pressure vessels,...

  14. 46 CFR 50.30-20 - Class III pressure vessels.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Class III pressure vessels. 50.30-20 Section 50.30-20... Fabrication Inspection § 50.30-20 Class III pressure vessels. (a) Class III pressure vessels shall be subject... specifically exempted by other regulations in this subchapter. (b) For Class III welded pressure vessels,...

  15. 46 CFR 50.30-20 - Class III pressure vessels.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Class III pressure vessels. 50.30-20 Section 50.30-20... Fabrication Inspection § 50.30-20 Class III pressure vessels. (a) Class III pressure vessels shall be subject... specifically exempted by other regulations in this subchapter. (b) For Class III welded pressure vessels,...

  16. Psychometric Testing of the FACES III with Rural Adolescents

    ERIC Educational Resources Information Center

    Ide, Bette; Dingmann, Colleen; Cuevas, Elizabeth; Meehan, Maurita

    2010-01-01

    This study tests the validity and reliability of the Family Adaptability and Cohesion Scale III (FACES III) in two samples of rural adolescents. The underlying theory is the linear 3-D circumplex model. The FACES III was administered to 1,632 adolescents in Grades 7 through 12 in two counties in a rural western state. The FACES III Scale and the…

  17. Molten-Salt-Based Growth of Group III Nitrides

    DOEpatents

    Waldrip, Karen E.; Tsao, Jeffrey Y.; Kerley, Thomas M.

    2008-10-14

    A method for growing Group III nitride materials using a molten halide salt as a solvent to solubilize the Group-III ions and nitride ions that react to form the Group III nitride material. The concentration of at least one of the nitride ion or Group III cation is determined by electrochemical generation of the ions.

  18. Teachers' Guide to Music Appreciation III A and III B in the Senior High School.

    ERIC Educational Resources Information Center

    Scott, J. Mark; Dawkins, Barbara R.

    This guide to music appreciation courses was developed for use in senior high schools in Duval County, Jacksonville, Florida. Music Appreciation III A examines the development of music, from the Gothic period through the Classical period. Music Appreciation III B examines the development of music from the Romantic period through the 1970s.…

  19. Hexaammine Complexes of Cr(III) and Co(III): A Spectral Study.

    ERIC Educational Resources Information Center

    Brown, D. R.; Pavlis, R. R.

    1985-01-01

    Procedures are provided for experiments containing complex ions with octahedral symmetry, hexaamminecobalt(III) chloride and hexaamminechromium(III) nitrate, so students can interpret fully the ultra violet/visible spectra of the complex cations in terms of the ligand field parameters, 10 "Dq," the Racah interelectron repulsion parameters, "B,"…

  20. Effects of difructose anhydride III (DFA III) administration on bile acids and growth of DFA III-assimilating bacterium Ruminococcus productus on rat intestine.

    PubMed

    Minamida, Kimiko; Kaneko, Maki; Ohashi, Midori; Sujaya, I Nengah; Sone, Teruo; Wada, Masaru; Yokota, Atsushi; Hara, Hiroshi; Asano, Kozo; Tomita, Fusao

    2005-06-01

    The growth of DFA III-assimilating bacteria in the intestines of rats fed 3% DFA III for 2 weeks was examined. Sixty-four percent of the DFA III intake had been assimilated on day 3 of ingestion, and almost all of the DFA III was assimilated at the end of the experiment. The DFA III-assimilating bacterium, Ruminococcus productus, in DFA III-fed rats was in the stationary state of 10(8)-10(9) cells/g dry feces within a week from 10(6) cells/g dry feces on day 1 of DFA III ingestion. The number of R. productus cells was associated with the amount of DFA III excreted in the feces. The acetic acid produced from DFA III by R. productus lowered the cecal pH to 5.8. In control-fed rats and DFA III-fed rats, 94% of secondary bile acids and 94% of primary bile acids, respectively, were accounted for in the total bile acids analyzed. DFA III ingestion increased the ratio of primary bile acids and changed the composition of fecal bile acids. In conclusion, R. productus assimilated DFA III, produced short chain fatty acids, and the cecal pH was lowered. The acidification of rat intestine perhaps inhibited secondary bile acid formation and decreased the ratio of secondary bile acids. Therefore, it is expected that DFA III may prevent colorectal cancer and be a new prebiotic candidate. PMID:16233830

  1. Class III viral membrane fusion proteins

    PubMed Central

    Backovic, Marija

    2010-01-01

    SUMMARY Accumulating structural studies of viral fusion glycoproteins have revealed unanticipated structural relationships between unrelated virus families and allowed the grouping of these membrane fusogens into three distinct classes. Here we review the newly identified group of class III viral fusion proteins, whose members include fusion proteins from rhabdoviruses, herpesviruses and baculoviruses. While clearly related in structure, the class III viral fusion proteins exhibit distinct structural features in their architectures as well as in their membrane-interacting fusion loops, which are likely related to their virus-specific differences in cellular entry. Further study of the similarities and differences in the class III viral fusion glycoproteins may provide greater insights into protein:membrane interactions that are key to promoting efficient bilayer fusion during virus entry. PMID:19356922

  2. The Spatial Infrared Imaging Telescope III.

    NASA Astrophysics Data System (ADS)

    Bartschi, B. Y.; Morse, D. E.; Woolston, T. L.

    1996-06-01

    The Spatial Infrared Imaging Telescope III (SPIRIT III) is a mid- through long-wave infrared instrumentation package built and managed by the Space Dynamics Laboratory at Utah State University for the Midcourse Space Experiment. SPIRIT III contains a radiometer and an auto-aligning interferometer-spectrometer that share a telescope designed for high off-axis rejection. A solid-hydrogen cryostat, the first of its kind to be used in a space/satellite application, cools the entire sensor system to cryogenic operating temperatures. This hardware will measure the spectral, spatial, temporal, and intensity characteristics of Earth-limb backgrounds, celestial objects, and other upper atmospheric phenomena. Collected data will provide answers to fundamental questions about Department of Defense surveillance systems and supply invaluable information for system planners and designers of future threat detection systems.

  3. Radiation shielding design considerations for Doublet III

    SciTech Connect

    Engholm, B.A.

    1980-06-01

    Calculations and measurements were made of the bremsstrahlung (x-ray) doses resulting from runaway electron shots at Doublet III. The analysis considered direct, wall-scattered, and skyshine contributions. Reasonably good agreement was obtained between calculations and measurements. The x-ray dose in the control room was about 1 mR per runaway shot, while that at the north boundary was undetectable, with a calculated value of 0.05 mR per shot. These low doses attest to the adequacy of the 2 ft concrete shadow shield surrounding the Doublet III room. Exploratory shielding analyses were performed for possible neutron generation if Doublet III were operated with neutral beam injection in an aggressive D-D mode.

  4. SAGE III Aerosol Extinction Validation in the Arctic Winter: Comparisons with SAGE II and POAM III

    NASA Technical Reports Server (NTRS)

    Thomason, L. W.; Poole, L. R.; Randall, C. E.

    2007-01-01

    The use of SAGE III multiwavelength aerosol extinction coefficient measurements to infer PSC type is contingent on the robustness of both the extinction magnitude and its spectral variation. Past validation with SAGE II and other similar measurements has shown that the SAGE III extinction coefficient measurements are reliable though the comparisons have been greatly weighted toward measurements made at mid-latitudes. Some aerosol comparisons made in the Arctic winter as a part of SOLVE II suggested that SAGE III values, particularly at longer wavelengths, are too small with the implication that both the magnitude and the wavelength dependence are not reliable. Comparisons with POAM III have also suggested a similar discrepancy. Herein, we use SAGE II data as a common standard for comparison of SAGE III and POAM III measurements in the Arctic winters of 2002/2003 through 2004/2005. During the winter, SAGE II measurements are made infrequently at the same latitudes as these instruments. We have mitigated this problem through the use potential vorticity as a spatial coordinate and thus greatly increased the number of coincident events. We find that SAGE II and III extinction coefficient measurements show a high degree of compatibility at both 1020 nm and 450 nm except a 10-20% bias at both wavelengths. In addition, the 452 to 1020-nm extinction ratio shows a consistent bias of approx. 30% throughout the lower stratosphere. We also find that SAGE II and POAM III are on average consistent though the comparisons show a much higher variability and larger bias than SAGE II/III comparisons. In addition, we find that the two data sets are not well correlated below 18 km. Overall, we find both the extinction values and the spectral dependence from SAGE III are robust and we find no evidence of a significant defect within the Arctic vortex.

  5. Complexation of Cm(III)/Eu(III) with Silicate in Basic Solutions

    SciTech Connect

    Wang, Zheming; Felmy, Andrew R; Xia, Yuanxian; Qafoku, Odeta; Yantasee, Wassana; Cho, Herman M

    2005-12-01

    The complexation of Cm(III) and Eu(III) with dissolved silica was studied by time resolved laser fluorescence spectroscopy (TRLFS) in basic solutions over a range of total silica concentrations and ionic strengths (NaNO3). In highly basic solutions, both the fluorescence spectra and lifetime data indicate the formation of Eu(III)/Cm(III) complexes with oligomeric silicates as well as hydroxide groups and/or nitrate in the presence of concentrated NaNO3. At high silica concentration the inner-sphere complexation caused the shift of the fluorescence spectral maximum for Cm(III)(aq) from 594 nm to up to 607 nm and a significant increase of the hypersensitive 5D0 → 7F2 band around 615 nm relative to the non-hypersensitive 5D0 → 7F1 band at 592 nm for Eu(III). At the same time, the fluorescence lifetime increased from 68 s to up to 202 s for Cm(III) in 0.1 M NaNO3 and from 115 s to 1.8 ms for Eu(III) in 3.0 M and 5.0 M NaNO3, consistent with the removal of 6 or more water molecules upon silicate complexation. Linear correlations between the spectral intensity of Cm(III) complexes and the concentrations of the dissolved silicates suggest that Cm(III) complexation with the silicate dimer, Si2O2(OH)22-, may play a role.

  6. Transcribing RNA polymerase III observed by electron cryomicroscopy.

    PubMed

    Hoffmann, Niklas A; Jakobi, Arjen J; Vorländer, Matthias K; Sachse, Carsten; Müller, Christoph W

    2016-08-01

    Electron cryomicroscopy reconstructions of elongating RNA polymerase (Pol) III at 3.9 Å resolution and of unbound Pol III (apo Pol III) in two distinct conformations at 4.6 Å and 4.7 Å resolution allow the construction of complete atomic models of Pol III and provide new functional insights into the adaption of Pol III to fulfill its specific transcription tasks. PMID:27059519

  7. Factor structure of the MCMI-III.

    PubMed

    Craig, R J; Bivens, A

    1998-02-01

    The factor structure of the Millon Clinical Multiaxial Inventory (Millon, 1994; MCMI-III) was assessed among 444 African American inpatient substance abusers and constitutes the first factor analysis of the MCMI-III. We found 3 main factors: General Maladjustment, Paranoid Behavior/Thinking With Detached Emotionality, and Antisocial Acting Out. These factors were essentially similar to previous findings of factor studies with the MCMI and MCMI-II across diverse populations. This factor invariance should lend credibility to the revised test and spur additional research into its psychometric properties. PMID:9615431

  8. Supported liquid membrane system for Cr(III) separation from Cr(III)/Cr(VI) mixtures.

    PubMed

    Religa, P; Rajewski, J; Gierycz, P; Swietlik, R

    2014-01-01

    This paper presents the results of analyses of the chromium(III) transport process from mixtures of Cr(III)/Cr(VI) ions using supported liquid membranes (SLM), in which dinonylnaphthalene sulfonic acid (DNNSA) and di(2-ethylhexyl) phosphoric acid (D2EHPA) were used as carriers. In both cases the membrane worked as a selective barrier for Cr(VI) ions. The increase in both the time of Cr(VI) ions-carrier interaction and the Cr(VI) concentration in the feed phase negatively influenced the Cr(III) separation. The polarizing layer consisting of Cr(VI) ions prevents the access of Cr(III) ions to the inter phase surface and leads to the deactivation of the carrier, which is the result of the strong oxidation properties of Cr(VI) ions. These factors meant that, in the case of the membrane with DNNSA, the membrane could not be used for the effective separation of Cr(III) from the Cr(III)/Cr(VI) mixture. On the other hand, the membrane with D2EHPA can be used for fast and efficient transport of Cr(III) ions, but only for strictly defined process parameters, i.e. where the level of chromium(VI) concentration is below 10(-3)M and with intensive feed phase mixing. PMID:24960010

  9. Academic Achievement of NCAA Division III Athletes

    ERIC Educational Resources Information Center

    Barlow, Kathy A.; Hickey, Ann

    2014-01-01

    A study of 215 athletes at a small private liberal arts Division III college revealed that athletes (a) begin their college experience with SATs no different from non-athletes; (b) attain GPAs that do not significantly differ from those of nonathletes; (c) achieve GPAs that do not significantly differ between their "in-season" semester…

  10. Perspectives of precipitation science: Part III

    NASA Astrophysics Data System (ADS)

    Michaelides, Silas

    2016-03-01

    This Special Issue of Atmospheric Research entitled "Perspectives of Precipitation Science: Part III" encompasses selected papers that were presented at the General Assembly of the European Geosciences Union, held in Vienna, Austria, from 27 April to 2 May 2014, within the framework of the (General) Session "Precipitation: Measurement, Climatology, Remote Sensing, and Modeling".

  11. Constraining the Statistics of Population III Binaries

    NASA Technical Reports Server (NTRS)

    Stacy, Athena; Bromm, Volker

    2012-01-01

    We perform a cosmological simulation in order to model the growth and evolution of Population III (Pop III) stellar systems in a range of host minihalo environments. A Pop III multiple system forms in each of the ten minihaloes, and the overall mass function is top-heavy compared to the currently observed initial mass function in the Milky Way. Using a sink particle to represent each growing protostar, we examine the binary characteristics of the multiple systems, resolving orbits on scales as small as 20 AU. We find a binary fraction of approx. 36, with semi-major axes as large as 3000 AU. The distribution of orbital periods is slightly peaked at approx. < 900 yr, while the distribution of mass ratios is relatively flat. Of all sink particles formed within the ten minihaloes, approx. 50 are lost to mergers with larger sinks, and 50 of the remaining sinks are ejected from their star-forming disks. The large binary fraction may have important implications for Pop III evolution and nucleosynthesis, as well as the final fate of the first stars.

  12. Photodetectors using III-V nitrides

    DOEpatents

    Moustakas, T.D.

    1998-12-08

    A bandpass photodetector using a III-V nitride and having predetermined electrical properties is disclosed. The bandpass photodetector detects electromagnetic radiation between a lower transition wavelength and an upper transition wavelength. That detector comprises two low pass photodetectors. The response of the two low pass photodetectors is subtracted to yield a response signal. 24 figs.

  13. International Space Programs. Aerospace Education III.

    ERIC Educational Resources Information Center

    Bulmer, S. B.

    This book, one in the series on Aerospace Education III, is a collection of the diverse information available regarding the international space programs. The five goals listed for the book are: to examine the Soviet space program, to understand the future of Soviet space activity, to examine other national and international space programs, to…

  14. Leveraging Information Technology. Track III: Organizational Issues.

    ERIC Educational Resources Information Center

    CAUSE, Boulder, CO.

    Seven papers from the 1987 CAUSE conference's Track III, Organizational Issues, are presented. They include: "Learning Resources and Technologies: A Unified Organizational Reorientation to Administering Educational Support Services" (Morrell D. Boone); "IRM: A Short-Lived Concept?" (James I. Penrod and Michael G. Dolence); "Organizing to Manage…

  15. ESEA Title III 1972 - PACE in Pennsylvania.

    ERIC Educational Resources Information Center

    Research and Information Services for Education, King of Prussia, PA.

    This document is a collection of abstracts of all ESEA Title III educational innovation projects funded or operating in Pennsylvania during 1972. Each abstract contains the name of the local supporting agency, the project number, financial information, target population, major objectives, activities, evaluation design, findings to date,…

  16. Photodetectors using III-V nitrides

    DOEpatents

    Moustakas, Theodore D.

    1998-01-01

    A bandpass photodetector using a III-V nitride and having predetermined electrical properties. The bandpass photodetector detects electromagnetic radiation between a lower transition wavelength and an upper transition wavelength. That detector comprises two low pass photodetectors. The response of the two low pass photodetectors is subtracted to yield a response signal.

  17. Type III functional response in Daphnia.

    PubMed

    Sarnelle, Orlando; Wilson, Alan E

    2008-06-01

    The functional response of Daphnia, a common pelagic herbivore in lakes, was assessed with a combination of secondary and meta-analyses of published data and new data from an experiment conducted using very low food levels. Secondary analyses of literature data (28 studies, n = 239-393) revealed a significant positive influence of food concentration on Daphnia clearance rate at low food levels, i.e., evidence of an overall Type III functional response. This result was not an artifact of including data from Daphnia that were exhausted from prolonged food deprivation (more than three hours at very low food). Meta-analysis of Daphnia clearance rate vs. food concentration across a range of low food concentrations (eight studies) showed a significantly positive slope across studies, which also supports the presence of a Type III response. Congruent with these analyses of published data, the feeding experiment showed clear evidence of a Type III functional response for D. pulicaria feeding on Ankistrodesmus falcatus. Food levels at which Daphnia clearance rate declined with decreasing food were near the minimum resource requirement for Daphnia population maintenance at steady state (R*). We suggest that Type III responses are more common than previously believed, perhaps because of the relative paucity of observations at low food levels, and that reduced prey mortality at low phytoplankton densities could be a stabilizing mechanism for Daphnia-phytoplankton systems under resource scarcity. PMID:18589536

  18. The Changing Nature of Division III Athletics

    ERIC Educational Resources Information Center

    Beaver, William

    2014-01-01

    Non-selective Division III institutions often face challenges in meeting their enrollment goals. To ensure their continued viability, these schools recruit large numbers of student athletes. As a result, when compared to FBS (Football Bowl Division) institutions these schools have a much higher percentage of student athletes on campus and a…

  19. Constraining the statistics of Population III binaries

    NASA Astrophysics Data System (ADS)

    Stacy, Athena; Bromm, Volker

    2013-08-01

    We perform a cosmological simulation in order to model the growth and evolution of Population III (Pop III) stellar systems in a range of host minihalo environments. A Pop III multiple system forms in each of the 10 minihaloes, and the overall mass function is top-heavy compared to the currently observed initial mass function in the Milky Way. Using a sink particle to represent each growing protostar, we examine the binary characteristics of the multiple systems, resolving orbits on scales as small as 20 au. We find a binary fraction of ˜35 per cent, with semi-major axes as large as 3000 au. The distribution of orbital periods is slightly peaked at ≲ 900 yr, while the distribution of mass ratios is relatively flat. Of all sink particles formed within the 10 minihaloes, ˜50 per cent are lost to mergers with larger sinks, and ˜50 per cent of the remaining sinks are ejected from their star-forming discs. The large binary fraction may have important implications for Pop III evolution and nucleosynthesis, as well as the final fate of the first stars.

  20. Estimation of the standard entropies of some Am(III) and Cm(III) compounds

    NASA Astrophysics Data System (ADS)

    Konings, R. J. M.

    2001-05-01

    The standard entropies S°(298.15 K) of some actinide(III) compounds have been estimated using a semi-empirical method describing the total entropy as the sum of the lattice entropy Slat and the excess entropy Sexs. The validity of the applied approach has been verified for the iso-electronic lanthanide(III) compounds for which a good agreement with experimental values has been obtained. The present results for the actinide(III) compounds are compared to previous estimates. Significant differences have been found, in particular for the americium compounds.

  1. The capillary pumped loop III (CAPL III) flight demonstration description and status

    NASA Astrophysics Data System (ADS)

    Kim, Jeong (Jake) H.; Cheung, Kwok-Hung; Butler, Dan; Ku, Jentung; Haught, Eric; Kroliczeck, Edward J.; Cullimore, Brent; Baumann, Jane

    1997-01-01

    To realize the full benefits of capillary pump loop (CPL) devices, for use in spacecraft thermal control subsystems, a reliable, load sharing, multiple evaporator system must be developed and successfully demonstrated in space. The Capillary Pumped Loop Flight Experiment 3 (CAPL III) will be the second attempt to flight demonstrate a multiple evaporator CPL in space environment. Using the lessons learned from CAPL I, which was flown aboard STS-60 in February 1994, new hardware and concepts are being developed for CAPL III to enable load sharing between evaporators, reliable system start-up/re-start, and reliable continuous operation. Started in May 1996, CAPL III is primarily a joint venture between the Naval Research Laboratory and the NASA-Goddard Space Flight Center, with Swales and Associates, Inc. as an industry partner. The program is scheduled to meet an STS flight opportunity in mid-1998. This paper will present the requirements and the preliminary design description of the CAPL III CPL system.

  2. Kinetics of the reaction between Pu(III) and Fe(III) in nitric acid solution

    SciTech Connect

    Koltunov, V.S.; Zhuravleva, G.I.

    1988-01-01

    The kinetics of the reaction between Pu/sup (III)/ and Fe/sup (III)/ in an aqueous nitric acid solution in the presence of persulfate ions at a constant ionic strength of the solution was studied spectrophotometrically. It was shown that the reaction is first order with respect to the reagents; its rate is practically independent of the concentration of the H/sup +/ and S/sub 2/O/sub 8//sup 2 -/ ions and with increase in the analytical HNO/sub 3/ concentration at variable ionic strength, it first increases, and then decreases. The temperature dependence of the reaction was determined, and its thermodynamic activation parameters were calculated. A possible mechanism for the reaction between Pu/sup (III)/ and Fe/sup (III)/ was proposed.

  3. Theoretical insights into the separation of Am(III) over Eu(III) with PhenBHPPA.

    PubMed

    Wu, Han; Wu, Qun-Yan; Wang, Cong-Zhi; Lan, Jian-Hui; Liu, Zhi-Rong; Chai, Zhi-Fang; Shi, Wei-Qun

    2015-10-14

    Due to the similar chemical properties of actinides An(iii) and lanthanides Ln(iii), their separation in spent nuclear fuel reprocessing is extremely challenging. A 1,10-phenanthroline dipicolinamide-based ligand (PhenBHPPA) has been identified to possess a selectivity for Am(iii) over Eu(iii) and could potentially be used for group actinide extraction. In this study, quasi-relativistic density functional theoretical calculations have been used to disclose the interaction mechanisms of Am(iii) and Eu(iii) complexes with PhenBHPPA. The electronic structures, bonding nature, QTAIM (Quantum Theory of Atoms in Molecules) analyses and thermodynamic behaviors of the Am(iii) and Eu(iii) complexes with PhenBHPPA have been explored in detail. According to the Wiberg bond indices (WBIs) and QTAIM analyses, interactions between the ligand and metal cations (Am(iii) and Eu(iii)) exhibit a weakly covalent character. Thermodynamic analyses show that the charged complexes [ML(NO3)2](+) appear to be the most stable species in the complexation processes. Moreover, it is more energetically favorable for PhenBHPPA to bind to Am(iii) compared to Eu(iii). Our study could render new insights into understanding the selectivity of the ligand towards minor actinides and the separation of An(iii) from Ln(iii) via liquid-liquid extraction. PMID:26332357

  4. Functional conservation of RNase III-like enzymes: studies on a Vibrio vulnificus ortholog of Escherichia coli RNase III.

    PubMed

    Lee, Minho; Ahn, Sangmi; Lim, Boram; Lee, Dong-Ho; Lee, Kangseok

    2014-04-01

    Bacterial ribonuclease III (RNase III) belongs to the RNase III enzyme family, which plays a pivotal role in controlling mRNA stability and RNA processing in both prokaryotes and eukaryotes. In the Vibrio vulnificus genome, one open reading frame encodes a protein homologous to E. coli RNase III, designated Vv-RNase III, which has 77.9 % amino acid identity to E. coli RNase III. Here, we report that Vv-RNase III has the same cleavage specificity as E. coli RNase III in vivo and in vitro. Expressing Vv-RNase III in E. coli cells deleted for the RNase III gene (rnc) restored normal rRNA processing and, consequently, growth rates of these cells comparable to wild-type cells. In vitro cleavage assays further showed that Vv-RNase III has the same cleavage activity and specificity as E. coli RNase III on RNase III-targeted sequences of corA and mltD mRNA. Our findings suggest that RNase III-like proteins have conserved cleavage specificity across bacterial species. PMID:24241331

  5. Rhodium(i), rhodium(iii) and iridium(iii) carbaporphyrins.

    PubMed

    Adiraju, Venkata A K; Ferrence, Gregory M; Lash, Timothy D

    2016-09-21

    Treatment of a benzocarbaporphyrin with [Rh(CO)2Cl]2 in refluxing dichloromethane gave a rhodium(i) dicarbonyl complex, and further reaction in refluxing pyridine afforded an organometallic rhodium(iii) derivative. The carbaporphyrin also reacted with [Ir(COD)Cl]2 and pyridine in refluxing p-xylene to generate a related iridium(iii) compound. These novel metalated porphyrinoids retained strongly diatropic characteristics and were fully characterized by XRD. PMID:27529466

  6. A XAFS study of plain and composite iron(III) and chromium(III) hydroxides.

    PubMed

    Papassiopi, N; Pinakidou, F; Katsikini, M; Antipas, G S E; Christou, C; Xenidis, A; Paloura, E C

    2014-09-01

    Reduction of hexavalent Cr(VI) to the trivalent state is the common strategy for remediation of Cr(VI) contaminated waters and soils. In the presence of Fe the resulting compounds are usually mixed Fe(III)-Cr(III) phases, while, under iron-free conditions, reduction leads to formation of plain Cr(III) hydroxides. Environmental stability of these compounds depends on their structure and is important to understand how different precipitation conditions affect the local atomic order of resulting compounds and thus their long term stability. In current study, typical Cr(VI) environmental remediation products, i.e. plain and mixed Fe(III)-Cr(III) hydroxides, were synthesized by hydrolysis and redox reactions and their structure was studied by X ray diffraction and X ray absorption fine structure techniques. Plain Cr(III) hydroxide was found to correspond to the molecular formula Cr(OH)3·3H2O and was identified as crystalline in XRD. However, the same compound when examined by EXAFS did not exhibit any clear local order in the range of EXAFS detectable distances, i.e. between 0 and 5Å. Namely, EXAFS spectroscopy detected only contribution from the first nearest neighboring (Cr-O) shell, suggesting that CrO6 octahedra interconnection is loose, in accordance with the suggested anti-bayerite structure of this compound. Mixed Fe(III)-Cr(III) systems resembled 2-line ferrihydrite irrespective of the synthesis route. Analysis of Fe-K-EXAFS and Cr-K-EXAFS spectra indicated that FeO6 octahedra are bonded by sharing both edges and corners, while CrO6 octahedra seem to prefer edge sharing linkage. EXAFS data also suggest that Fe-Cr hydroxide produced by hydrolysis presents a better arrangement of CrO6 octahedra compared to the redox product. PMID:24997915

  7. Characterization of Human RNA Polymerase III Identifies Orthologues for Saccharomyces cerevisiae RNA Polymerase III Subunits

    PubMed Central

    Hu, Ping; Wu, Si; Sun, Yuling; Yuan, Chih-Chi; Kobayashi, Ryuji; Myers, Michael P.; Hernandez, Nouria

    2002-01-01

    Unlike Saccharomyces cerevisiae RNA polymerase III, human RNA polymerase III has not been entirely characterized. Orthologues of the yeast RNA polymerase III subunits C128 and C37 remain unidentified, and for many of the other subunits, the available information is limited to database sequences with various degrees of similarity to the yeast subunits. We have purified an RNA polymerase III complex and identified its components. We found that two RNA polymerase III subunits, referred to as RPC8 and RPC9, displayed sequence similarity to the RNA polymerase II RPB7 and RPB4 subunits, respectively. RPC8 and RPC9 associated with each other, paralleling the association of the RNA polymerase II subunits, and were thus paralogues of RPB7 and RPB4. Furthermore, the complex contained a prominent 80-kDa polypeptide, which we called RPC5 and which corresponded to the human orthologue of the yeast C37 subunit despite limited sequence similarity. RPC5 associated with RPC53, the human orthologue of S. cerevisiae C53, paralleling the association of the S. cerevisiae C37 and C53 subunits, and was required for transcription from the type 2 VAI and type 3 human U6 promoters. Our results provide a characterization of human RNA polymerase III and show that the RPC5 subunit is essential for transcription. PMID:12391170

  8. III-V nanowire growth mechanism: V/III ratio and temperature effects.

    PubMed

    Dayeh, Shadi A; Yu, Edward T; Wang, Deli

    2007-08-01

    We have studied the dependence of Au-assisted InAs nanowire (NW) growth on InAs(111)B substrates as a function of substrate temperature and input V/III precursor ratio using organometallic vapor-phase epitaxy. Temperature-dependent growth was observed within certain temperature windows that are highly dependent on input V/III ratios. This dependence was found to be a direct consequence of the drop in NW nucleation and growth rate with increasing V/III ratio at a constant growth temperature due to depletion of indium at the NW growth sites. The growth rate was found to be determined by the local V/III ratio, which is dependent on the input precursor flow rates, growth temperature, and substrate decomposition. These studies advance understanding of the key processes involved in III-V NW growth, support the general validity of the vapor-liquid-solid growth mechanism for III-V NWs, and improve rational control over their growth morphology. PMID:17608541

  9. Adsorption behavior of Eu(III) on partially Fe(III)- or Ti(IV)-coated silica

    PubMed Central

    2012-01-01

    The adsorption behavior of Eu(III) onto silica surface, which was partially coated with Fe(III) or Ti(IV), was investigated to determine Fe(III) or Ti(IV) effects on the surface reaction of lanthanides on mineral surfaces in groundwater. Compared with a parallel uncoated silica, the Fe(III)-coated silica did not enhance the adsorption of Eu(III). However, enhanced adsorption of Eu(III) on the Ti(IV)-coated silica was observed by increasing the amount of Ti(IV) on the silica surface. PMID:22221407

  10. Hyper III on ramp, front view

    NASA Technical Reports Server (NTRS)

    1969-01-01

    The Hyper III was a low-cost test vehicle for an advanced lifting-body shape. Like the earlier M2-F1, it was a 'homebuilt' research aircraft, i.e., built at the Flight Research Center (FRC), later redesignated the Dryden Flight Research Center. It had a steel-tube frame covered with Dacron, a fiberglass nose, sheet aluminum fins, and a wing from an HP-11 sailplane. Construction was by volunteers at the FRC. Although the Hyper III was to be flown remotely in its initial tests, it was fitted with a cockpit for a pilot. On the Hyper III's only flight, it was towed aloft attached to a Navy SH-3 helicopter by a 400-foot cable. NASA research pilot Bruce Peterson flew the SH-3. After he released the Hyper III from the cable, NASA research pilot Milt Thompson flew the vehicle by radio control until the final approach when Dick Fischer took over control using a model-airplane radio-control box. The Hyper III flared, then landed and slid to a stop on Rogers Dry Lakebed. The Flight Research Center (FRC--as Dryden was named from 1959 until 1976) already had experience with testing small-scale aircraft using model-airplane techniques, but the first true remotely piloted research vehicle was the Hyper III, which flew only once in December 1969. At that time, the Center was engaged in flight research with a variety of reentry shapes called lifting bodies, and there was a desire both to expand the flight research experience with maneuverable reentry vehicles, including a high-performance, variable-geometry craft, and to investigate a remotely piloted flight research technique that made maximum use of a research pilot's skill and experience by placing him 'in the loop' as if he were in the cockpit. (There have been, as yet, no female research pilots assigned to Dryden.) The Hyper III as originally conceived was a stiletto-shaped lifting body that had resulted from a study at NASA's Langley Research Center in Hampton, Virginia. It was one of a number of hypersonic, cross

  11. Background investigation in EDELWEISS-III

    NASA Astrophysics Data System (ADS)

    Scorza, Silvia

    2015-08-01

    Protection from and rejection of backgrounds is a key issue for the EDELWEISS-III direct dark matter detection experiment which aims at exploring the 10-9 pb cross-section region for spin-independent WIMP-nucleon interaction. The detector is located in the low radioactivity environment of the Modane Underground Laboratory and consists of 36 advanced FID germanium detectors operating at 18 mK in a dilution refrigerator in order to identify eventual rare nuclear recoils induced by elastic scattering of WIMPs from our Galactic halo. I will discuss the background and the methods of rejecting it with the FID detectors. Detector performances and the first analysis of data acquired in a long-term campaign will be presented as well. The FID detector technology is not limited to EDELWEISS-III but can further be employed in the next generation of cryogenic detector experiments.

  12. Doublet III beamline: as-built

    SciTech Connect

    Harder, C.R.; Holland, M.M.; Parker, J.W.; Gunn, J.; Resnick, L.

    1980-03-01

    In order to fully exploit Doublet III capabilities and to study new plasma physics regimes, a Neutral Beam Injector System has been constructed. Initially, a two beamline system will supply 7 MW of heat to the plasma. The system is currently being expanded to inject approx. 20 MW of power (6 beamlines). Each beamline is equipped with two Lawrence Berkeley Laboratory type rectangular ion sources with 10 cm x 40 cm extraction grids. These sources will accelerate hydrogen ions to 80 keV, with extracted beam currents in excess of 80 A per source expected. The first completed source is currently being tested and conditioned on the High Voltage Test Stand at Lawrence Livermore Laboratory. This paper pictorially reviews the as-built Doublet III neutral beamline with emphasis on component relation and configuration relative to spatial and source imposed design constraints.

  13. Mucolipidosis Type III α/β

    PubMed Central

    Kerr, Darcy A.; Memoli, Vincent A.; Cathey, Sara S.; Harris, Brent T.

    2014-01-01

    We report findings from an autopsy of a 45-year-old woman with the rare lysosomal storage disease mucolipidosis type III α/β. Her disease manifested most notably as multiple bone and cartilage problems with tracheal and bronchial malacia. Principal autopsy findings included gross abnormalities in bone and cartilage with corresponding microscopic cytoplasmic lysosomal granules. These cytoplasmic granules were also seen in histologic preparations of the brain, myocardium, heart valves, and fibroblasts of the liver and skin by light and electron microscopy. By electron microscopy there were scattered, diffuse vesicular cytoplasmic granules in neurons and glia and an increase in lysosomal structures with fine electron lucent granularity in the above tissue types. Our findings help elaborate current understanding of this disease and differentiate it from the mucopolysaccharidoses and related disorders. To our knowledge, this is the first report to document pathologic findings in a patient with mucolipidosis type III α/β by autopsy. PMID:21466370

  14. Flexor tendon repair in zone III.

    PubMed

    Al-Qattan, Mohammad M

    2011-01-01

    There is a paucity of the literature on the outcome of zone III flexor tendon injuries. In this paper, we report on the results of zone III flexor tendon repair in 35 consecutive adult patients with clean cut lacerations of both flexor tendons in 42 fingers. There were 25 men and 10 women with an average age of 32 years. Repair of both flexor tendons was performed using 'figure of eight' core sutures and a continuous epitendinous suture. Postoperatively, an immediate active range of motion protocol was applied to ensure full active extension of the interphalangeal joints. The results were assessed using the Strickland-Glogovac grading system. There were no ruptures. One patient with two injured fingers developed complex regional pain syndrome and the final outcome was fair in both fingers. In the remaining 34 patients (40 fingers), 33 patients (38 fingers) had an excellent outcome and the remaining patient (two fingers) had a good outcome. PMID:20807720

  15. Luminescence of europium (III) complexes for visualization

    NASA Astrophysics Data System (ADS)

    Kolontaeva, Olga A.; Pozharov, Mikhail V.; Korolovich, Vladimir F.; Khokhlova, Anastasia R.; Kirdyanova, Anna N.; Burmistrova, Natalia A.; Zakharova, Tamara V.; Goryacheva, Irina Y.

    2016-04-01

    With the purpose to develop bright non-toxic luminescent label for theranostic application we have studied complexation of lanthanide dipicolinates (2,6-pyridinedicarboxylates) by sodium alginate and effect of thermal exposure of synthesized micro-capsules on their luminescent properties. Synthesized micro-capsules are stable in acidic medium but dissolve at pH ~ 4 due to transformation of cationic europium dipicolinate complex to anionic. Luminescence studies have shown that emission spectra of europium(III)-alginate complexes (both chloride and dipicolinate) contain two intensive bands characteristic to Eu3+ ion (5D0 --> 7F1 (590 nm) and 5D0 --> 7F1 (612 nm)). We have also found that at 160ºC europium(III)- alginate micro-capsules decompose to black, soot-like substance, therefore, their thermal treatment must be performed in closed environment (i.e., sealed ampoules).

  16. LSPRAY-III: A Lagrangian Spray Module

    NASA Technical Reports Server (NTRS)

    Raju, M. S.

    2008-01-01

    LSPRAY-III is a Lagrangian spray solver developed for application with parallel computing and unstructured grids. It is designed to be massively parallel and could easily be coupled with any existing gas-phase flow and/or Monte Carlo Probability Density Function (PDF) solvers. The solver accommodates the use of an unstructured mesh with mixed elements of either triangular, quadrilateral, and/or tetrahedral type for the gas flow grid representation. It is mainly designed to predict the flow, thermal and transport properties of a rapidly vaporizing spray because of its importance in aerospace application. The manual provides the user with an understanding of various models involved in the spray formulation, its code structure and solution algorithm, and various other issues related to parallelization and its coupling with other solvers. With the development of LSPRAY-III, we have advanced the state-of-the-art in spray computations in several important ways.

  17. Background investigation in EDELWEISS-III

    SciTech Connect

    Scorza, Silvia

    2015-08-17

    Protection from and rejection of backgrounds is a key issue for the EDELWEISS-III direct dark matter detection experiment which aims at exploring the 10{sup −9} pb cross-section region for spin-independent WIMP-nucleon interaction. The detector is located in the low radioactivity environment of the Modane Underground Laboratory and consists of 36 advanced FID germanium detectors operating at 18 mK in a dilution refrigerator in order to identify eventual rare nuclear recoils induced by elastic scattering of WIMPs from our Galactic halo. I will discuss the background and the methods of rejecting it with the FID detectors. Detector performances and the first analysis of data acquired in a long-term campaign will be presented as well. The FID detector technology is not limited to EDELWEISS-III but can further be employed in the next generation of cryogenic detector experiments.

  18. Chukar III-R Reconnaissance System

    NASA Astrophysics Data System (ADS)

    Toops, Laurence C.

    1990-02-01

    This paper describes Northrop's developmental Chukar 111-R reconnaissance system, which is based on the Chukar III target drone. Some military needs for reconnaissance and the advantages of employing an unmanned air vehicle to satisfy these needs are noted. Next, features incorporated into the new Chukar III-R reconnaissance system are described. These features include a high performance unmanned air vehicle (UAV), an infrared line scanner for imaOng targets, radio position-fix enhanced navigation, and a new mission planning and control station. Sensor slight test results, a pay-load mockup, and mission planning and image exploitation capabilities are discussed. The advantages of high speed and low observability are cited. Launch and retrieval techniques are described.

  19. MAVIS III -- A Windows 95/NT Upgrade

    SciTech Connect

    Hardwick, M.F.

    1997-12-01

    MAVIS (Modeling and Analysis of Explosive Valve Interactions) is a computer program that simulates operation of explosively actuated valve. MAVIS was originally written in Fortran in the mid 1970`s and was primarily run on the Sandia Vax computers in use through the early 1990`s. During the mid to late 1980`s MAVIS was upgraded to include the effects of plastic deformation and it became MAVIS II. When the Vax computers were retired, the Gas Transfer System (GTS) Development Department ported the code to the Macintosh and PC platforms, where it ran as a simple console application. All graphical output was lost during these ports. GTS code developers recently completed an upgrade that provides a Windows 95/NT MAVIS application and restores all of the original graphical output. This upgrade is called MAVIS III version 1.0. This report serves both as a user`s manual for MAVIS III v 1.0 and as a general software development reference.

  20. Antisites in III-V semiconductors: Density functional theory calculations

    NASA Astrophysics Data System (ADS)

    Chroneos, A.; Tahini, H. A.; Schwingenschlögl, U.; Grimes, R. W.

    2014-07-01

    Density functional based simulation, corrected for finite size effects, is used to investigate systematically the formation of antisite defects in III-V semiconductors (III = Al, Ga, and In and V = P, As, and Sb). Different charge states are modelled as a function of the Fermi level and under different growth conditions. The formation energies of group III antisites ( III V q) decrease with increasing covalent radius of the group V atom though not group III radius, whereas group V antisites ( V I I I q) show a consistent decrease in formation energies with increase in group III and group V covalent radii. In general, III V q defects dominate under III-rich conditions and V I I I q under V-rich conditions. Comparison with equivalent vacancy formation energy simulations shows that while antisite concentrations are always dominant under stoichiometric conditions, modest variation in growth or doping conditions can lead to a significantly higher concentration of vacancies.

  1. Engineering design of ARIES-III

    SciTech Connect

    Sze, D.K.; Wong, C.; Cheng, E.

    1993-07-01

    An efficient organic cooled low activation ferritic steel first wall and shield has been designed for the D-{sup 3}He power reactor ARIES-III. The design allows removal of the large surface heat load without exceeding temperature and stress design limits. The structure is expected to last for the whole reactor life. The major concerns regarding using the organic coolant in fusion reactors have been greatly alleviated.

  2. Organic coolant for ARIES-III

    SciTech Connect

    Sze, D.K. ); Sviatoslavsky, I.; Sawan, M. ); Gierszewski, P. ); Hollies, R. ); Sharafat, S. ); Herring, S. )

    1991-04-01

    ARIES-III is a D-He{sub 3} reactor design study. It is found that the organic coolant is well suited for the D-He{sub 3} reactor. This paper discusses the unique features of the D-He{sub 3} reactor, and the reason that the organic coolant is compatible with those features. The problems associated with the organic coolant are also discussed. 8 refs., 2 figs., 6 tabs.

  3. Biosorption of Cr(III) and Fe(III) in single and binary systems onto pretreated orange peel.

    PubMed

    Lugo-Lugo, V; Barrera-Díaz, C; Ureña-Núñez, F; Bilyeu, B; Linares-Hernández, I

    2012-12-15

    Trivalent chromium and iron are the products of the traditional reduction of hexavalent chromium by ferrous salts in industrial wastewater. Although there have been a few studies of Cr(III) adsorption, none have considered the effect of Fe(III) on Cr(III) adsorption in a binary system representing expected products of hexavalent chromium in industrial wastewater. The biosorption of Cr(III) and Fe(III) ions onto pretreated ground orange peel in single and binary systems was studied in batch experiments using a variety of techniques. The kinetic results showed a rapid rate of biosorption of Cr(III) and Fe(III) in single and binary systems and mutual interference effects in the competitive binary Cr(III)-Fe(III) system. Second order kinetic models showed the best fit for all systems. The behavior of competitive Cr(III)-Fe(III) biosorption were successfully described by the multicomponent Langmuir model, obtaining maximum capacities for Cr(III) and Fe(III) of 9.43 and 18.19 mg/g respectively. SEM/EDS results confirmed that the metals adsorb on the surface and FTIR identified the hydroxyl groups on the carboxylic acids as the active binding sites. PMID:22892173

  4. First heterometallic Ga(III)-Dy(III) single-molecule magnets: implication of Ga(III) in extracting Fe-Dy interaction.

    PubMed

    Chen, Sihuai; Mereacre, Valeriu; Anson, Christopher E; Powell, Annie K

    2016-05-31

    The compounds of the system [M4M'2(μ3-OH)2(nbdea)4(C6H5CO2)8]·MeCN, where M = Ga(III), M' = Dy(III) (), M = Fe(III), M' = Y(III) () are isostructural to the known [Fe4Dy2] compound (). Those of the system [M4M'4(μ3-OH)4(nbdea)4(m-CH3C6H4CO2)12]·nMeCN, where M = Ga(III), M' = Dy(III), n = 4 (), M = Fe(III), M' = Y(III), n = 1 () are isostructural to the [Fe4Dy4] compound (). This allows for comparisons between single ion effects of the paramagnetic ions. The structures were determined using single crystal analysis. Magnetic susceptibility measurements reveal that the Ga(III)-Dy(III) compounds and are SMMs. The energy barrier for is close to that for the known isostructural Fe4Dy2 compound (), but with a significantly increased relaxation time. PMID:27184869

  5. Dry etching of III-V nitrides

    SciTech Connect

    Pearton, S.J.; Shul, R.J.; McLane, G.F.; Constantine, C.

    1995-12-01

    The chemical inertness and high bond strengths of the III-V nitrides lead to slower plasma etching rates than for more conventional III-V semiconductors under the same conditions. High ion density conditions (>3{times}l0{sup 9}cm{sup {minus}3}) such as those obtained in ECR or magnetron reactors produce etch rates up to an order of magnitude higher than for RIE, where the ion densities are in the 10{sup 9}cm{sup {minus}3} range. We have developed smooth anisotropic dry etches for GaN, InN, AlN and their alloys based on Cl{sub 2}/CH{sub 4}/H{sub 2}/Ar, BCl{sub 3}/Ar, Cl{sub 2}/H{sub 2}, Cl{sub 2}/SF{sub 6}, HBr/H{sub 2} and HI/H{sub 2} plasma chemistries achieving etch rates up to {approximately}4,000{angstrom}/min at moderate dc bias voltages ({le}-150V). Ion-induced damage in the nitrides appears to be less apparent than in other III-V`s. One of the key remaining issues is the achievement of high selectivities for removal of one layer from another.

  6. Energy source of flagellar type III secretion.

    PubMed

    Paul, Koushik; Erhardt, Marc; Hirano, Takanori; Blair, David F; Hughes, Kelly T

    2008-01-24

    Bacterial flagella contain a specialized secretion apparatus that functions to deliver the protein subunits that form the filament and other structures to outside the membrane. This apparatus is related to the injectisome used by many gram-negative pathogens and symbionts to transfer effector proteins into host cells; in both systems this export mechanism is termed 'type III' secretion. The flagellar secretion apparatus comprises a membrane-embedded complex of about five proteins, and soluble factors, which include export-dedicated chaperones and an ATPase, FliI, that was thought to provide the energy for export. Here we show that flagellar secretion in Salmonella enterica requires the proton motive force (PMF) and does not require ATP hydrolysis by FliI. The export of several flagellar export substrates was prevented by treatment with the protonophore CCCP, with no accompanying decrease in cellular ATP levels. Weak swarming motility and rare flagella were observed in a mutant deleted for FliI and for the non-flagellar type-III secretion ATPases InvJ and SsaN. These findings show that the flagellar secretion apparatus functions as a proton-driven protein exporter and that ATP hydrolysis is not essential for type III secretion. PMID:18216859

  7. 1,2,4-Diazaphospholide complexes of yttrium(iii), dysprosium(iii), erbium(iii), and europium(ii,iii): synthesis, X-ray structural characterization, and EPR analysis.

    PubMed

    Wang, Yongli; Guo, Wenzhen; Liu, Dongling; Yang, Ying; Zheng, Wenjun

    2016-01-21

    Several structurally characterized heteroleptic, charge-separated heterobimetallic, and polymeric alkali metal ate complexes of 1,2,4-diazaphospholide Y(iii), Dy(iii), Er(iii), Eu(iii), and Eu(ii) were prepared via the reaction of MCl3 and K[3,5-R2dp] in varied ratios at 200-220 °C (M = Y, Dy, Er, Eu; R = tBu, Ph). PMID:26666366

  8. 1,2,4-Diazaphospholide complexes of lanthanum(iii), cerium(iii), neodymium(iii), praseodymium(iii), and samarium(iii): synthesis, X-ray structural characterization, and magnetic susceptibility studies.

    PubMed

    Zhao, Minggang; Wang, Lixia; Li, Pangpang; Ma, Jianping; Zheng, Wenjun

    2016-07-01

    A few heteroleptic, charge-separated heterobimetallic, and polymeric alkali metalate complexes of 1,2,4-diazaphospholide lanthanum(iii), cerium(iii), neodymium(iii), praseodymium(iii), and samarium(iii) were simply prepared via the metathesis reaction of MCl3 (THF)m (m = 1-2) and K[3,5-R2dp] ([3,5-R2dp](-) = 3,5-di-substituent-1,2,4-diazaphospholide; R = tBu, Ph) in a varied ratio (1 : 3, 1 : 4, and 1 : 5, respectively) at room temperature in tetrahydrofuran. All the complexes were fully characterized by (1)H, (13)C{(1)H}, (31)P{(1)H}, IR, and X-ray single crystal diffraction analysis despite their paramagnetism (excluding La(iii) complexes). The structures of the complexes were found to feature varied coordination modes. The magnetic properties of several compounds were studied by magnetic susceptibility, and the complexes presented the magnetic moments close to or lower than the theoretical values for the free ions in the trivalent oxidation states (Pr(3+), Nd(3+)). PMID:27326667

  9. 49 CFR 172.440 - RADIOACTIVE YELLOW-III label.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 2 2012-10-01 2012-10-01 false RADIOACTIVE YELLOW-III label. 172.440 Section 172... REQUIREMENTS, AND SECURITY PLANS Labeling § 172.440 RADIOACTIVE YELLOW-III label. (a) Except for size and color, the RADIOACTIVE YELLOW-III label must be as follows: EC02MR91.034 (b) In addition to complying...

  10. 49 CFR 172.440 - RADIOACTIVE YELLOW-III label.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 2 2011-10-01 2011-10-01 false RADIOACTIVE YELLOW-III label. 172.440 Section 172... REQUIREMENTS, AND SECURITY PLANS Labeling § 172.440 RADIOACTIVE YELLOW-III label. (a) Except for size and color, the RADIOACTIVE YELLOW-III label must be as follows: EC02MR91.034 (b) In addition to complying...

  11. 49 CFR 172.440 - RADIOACTIVE YELLOW-III label.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 2 2013-10-01 2013-10-01 false RADIOACTIVE YELLOW-III label. 172.440 Section 172... REQUIREMENTS, AND SECURITY PLANS Labeling § 172.440 RADIOACTIVE YELLOW-III label. (a) Except for size and color, the RADIOACTIVE YELLOW-III label must be as follows: EC02MR91.034 (b) In addition to complying...

  12. 49 CFR 172.440 - RADIOACTIVE YELLOW-III label.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false RADIOACTIVE YELLOW-III label. 172.440 Section 172... REQUIREMENTS, AND SECURITY PLANS Labeling § 172.440 RADIOACTIVE YELLOW-III label. (a) Except for size and color, the RADIOACTIVE YELLOW-III label must be as follows: EC02MR91.034 (b) In addition to complying...

  13. 49 CFR 172.440 - RADIOACTIVE YELLOW-III label.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 2 2014-10-01 2014-10-01 false RADIOACTIVE YELLOW-III label. 172.440 Section 172... REQUIREMENTS, AND SECURITY PLANS Labeling § 172.440 RADIOACTIVE YELLOW-III label. (a) Except for size and color, the RADIOACTIVE YELLOW-III label must be as follows: EC02MR91.034 (b) In addition to complying...

  14. Korean Cultural Influences on the Millon Clinical Multiaxial Inventory III.

    ERIC Educational Resources Information Center

    Gunsalus, Ae-Jung Chang; Kelly, Kevin R.

    2001-01-01

    Investigates the effect of Korean culture on the results of the Millon Clinical Multiaxial Inventory-III (MCMI-III) by comparing profiles of 147 Korean and 132 American college students. Results indicate that MCMI-III personality profile differences exist between Korean and American college students. Discusses implications for mental health…

  15. Brazilian Adaptation of the Woodcock-Johnson III Cognitive Tests

    ERIC Educational Resources Information Center

    Wechsler, Solange Muglia; Nunes, Carlos Sancineto; Schelini, Patricia Waltz; Pasian, Sonia Regina; Homsi, Silvia Vertoni; Moretti, Lucia; Anache, Alexandra Ayach

    2010-01-01

    An adaptation of the standard battery of Woodcock-Johnson III Tests of Cognitive Abilities (WJ-III) for Brazilian children and youth was investigated. The sample was composed of 1094 students (54 percent girls), ages 7-17, living in Sao Paulo state (91 percent). Items from Brazilian school books as well as from the WJ-III Spanish version…

  16. Current Use of DSM-III-R in Counselor Education.

    ERIC Educational Resources Information Center

    Ritchie, Martin H.; And Others

    1991-01-01

    Surveyed 146 counselor education programs to investigate proportion of programs that teach use of "Diagnostic and Statistical Manual of Mental Disorders-3rd Edition," revised (DSM-III-R), program tracks requiring DSM-III-R training, and differences between accredited and nonaccredited programs in DSM-III-R coverage. Found that most programs taught…

  17. 40 CFR Appendix III to Part 261 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false III Appendix III to Part 261 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE Appendix III to Part 261...

  18. Microbial As(III) Oxidation in Water Treatment Plant Filters

    EPA Science Inventory

    Arsenic exists in two oxidation states in water - arsenite [As(III)] and arsenate [As(V)]. As(III) is relatively mobile in water and difficult to remove by arsenic-removal treatment processes. Source waters that contain As(III) must add a strong oxidant such as free chlorine or p...

  19. 76 FR 60511 - Amendment of Marine Safety Manual, Volume III

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-29

    ... Federal Register (73 FR 3316). Background and Purpose Chapter 16 of Volume III of the Marine Safety Manual... SECURITY Coast Guard Amendment of Marine Safety Manual, Volume III AGENCY: Coast Guard, DHS. ACTION: Notice... Offshore Units. The policy is currently found in Chapter 16 of the Marine Safety Manual, Volume III....

  20. 76 FR 33589 - Standards Improvement Project-Phase III

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ...Phase III of the Standards Improvement Project (SIP-III) is the third in a series of rulemaking actions to improve and streamline OSHA standards. The Standards Improvement Project removes or revises individual requirements within rules that are confusing, outdated, duplicative, or inconsistent. OSHA identified several requirements for SIP-III (e.g., rigging, NIOSH records, and training......

  1. Luminescent properties of europium(III) and terbium(III) complexes with para- and ortho-ethoxybenzoic acids

    NASA Astrophysics Data System (ADS)

    Panyushkin, V. T.; Mutuzova, M. Kh.; Shamsutdinova, M. Kh.

    2016-02-01

    The luminescent properties of europium(III) and terbium(III) complexes with para- and ortho-ethoxybenzoic acids are studied. The excitation energies of the triplet states of ligands are determined, a hypothesis is made about the efficient luminescence of europium(III) and terbium(III) complexes, the geometry of the coordination polyhedron of a europium complex is established, and the luminescence quantum yields of the complexes in solution are determined.

  2. [Ln(III)-Mn(II)-Ln(III)] heterometallic compounds: rare linear SMMs with divalent manganese ions.

    PubMed

    Li, Xiao-Lei; Min, Fan-Yong; Wang, Chao; Lin, Shuang-Yan; Liu, Zhiliang; Tang, Jinkui

    2015-02-21

    The reaction of Mn(OAc)2·4H2O and Ln(NO3)3·6H2O with N-(2-aminopropyl)-2-hydroxybenzamide and salicylic aldehyde in methanol/methylene dichloride produces yellow crystals of Ln2Mn(C7H5O2)8 (Ln = Gd (), Tb (), Dy (), Ho () and Er ()), in the presence of triethylamine. Three metal ions are connected by six μ2-phenolate oxygen atoms of six salicylic aldehyde ligands, resulting in perfect linear [Ln(III)-Mn(II)-Ln(III)] structures. Magnetic studies of these complexes have been performed and AC susceptibility measurements show the presence of a temperature-dependent out-of-phase ac signal for complexes and indicating single-molecule magnet (SMM) behavior. The Dy(III)2Mn(II) compound shows double relaxation pathways which may originate from the single ion behavior of individual Dy(III) ions and the weak coupling between Dy(III) and Mn(II) ions, respectively. The Ueff of 92.4(2) K is a relatively high value among 3d-4f SMMs. Moreover, complexes and represent the first linear Mn-Ln SMMs containing only divalent manganese ions as far as we know. The result suggests the positive effects of magnetic coupling to enhance their SMM behavior, presenting a promising strategy for constructing efficient heterometallic SMMs. PMID:25601415

  3. Does As(III) interact with Fe(II), Fe(III) and organic matter through ternary complexes?

    PubMed

    Catrouillet, Charlotte; Davranche, Mélanie; Dia, Aline; Bouhnik-Le Coz, Martine; Demangeat, Edwige; Gruau, Gérard

    2016-05-15

    Up until now, only a small number of studies have been dedicated to the binding processes of As(III) with organic matter (OM) via ionic Fe(III) bridges; none was interested in Fe (II). Complexation isotherms were carried out with As(III), Fe(II) or Fe(III) and Leonardite humic acid (HA). Although PHREEQC/Model VI, implemented with OM thiol groups, reproduced the experimental datasets with Fe(III), the poor fit between the experimental and modeled Fe(II) data suggested another binding mechanism for As(III) to OM. PHREEQC/Model VI was modified to take various possible As(III)-Fe(II)-OM ternary complex conformations into account. The complexation of As(III) as a mononuclear bidentate complex to a bidentate Fe(II)-HA complex was evidenced. However, the model needed to be improved since the distribution of the bidentate sites appeared to be unrealistic with regards to the published XAS data. In the presence of Fe(III), As(III) was bound to thiol groups which are more competitive with regards to the low density of formed Fe(III)-HA complexes. Based on the new data and previously published results, we propose a general scheme describing the various As(III)-Fe-MO complexes that are able to form in Fe and OM-rich waters. PMID:26939079

  4. The role of Ce(III) in BZ oscillating reactions

    NASA Astrophysics Data System (ADS)

    Nogueira, Paulo A.; Varela, Hamilton; Faria, Roberto B.

    2012-03-01

    Herein we present results on the oscillatory dynamics in the bromate-oxalic acid-acetone-Ce(III)/Ce(IV) system in batch and also in a CSTR. We show that Ce(III) is the necessary reactant to allow the emergence of oscillations. In batch, oscillations occur with Ce(III) and also with Ce(IV), but no induction period is observed with Ce(III). In a CSTR, no oscillations were found using a freshly prepared Ce(IV), but only when the cerium-containing solution was aged, allowing partial conversion of Ce(IV) to Ce(III) by reaction with acetone.

  5. Antioxidant property of quercetin-Cr(III) complex: The role of Cr(III) ion

    NASA Astrophysics Data System (ADS)

    Chen, Weijun; Sun, Shaofang; cao, Wei; Liang, Yan; Song, Jirong

    2009-01-01

    Flavonoid-metal complex is reported to exhibit a higher antioxidant activity than parent flavonoid. In this paper, experimental and theoretical methods are applied to study the antioxidant properties of quercetin and quercetin-Cr(III) complex, to find out the antioxidant activity variation and the role of Cr(III) ion on the antioxidant activity of the complex. Bond dissociation energy (BDE) and ionization potential (IP) of quercetin and the complex are calculated at the B3LYP/6-311++G(2d,2p)//B3LYP/LANL2DZ level. The experimental results show that the complex has a higher DPPH radical scavenging activity than quercetin. The calculated results show that the complex displays lower BDE and IP than quercetin. The IP of the complex declines obviously, indicating that the Cr (III) ion has more impact on the electron donating ability than on the hydrogen atom transferring ability of the complex.

  6. Redox stability of As(III) on schwertmannite surfaces.

    PubMed

    Paikaray, Susanta; Essilfie-Dughan, Joseph; Göttlicher, Jörg; Pollok, Kilian; Peiffer, Stefan

    2014-01-30

    As(III)-enriched mine discharge often drains through Fe(III)-mineral abundant land covers which makes the understanding of its fate and redox behaviour extremely important. We therefore conducted batch kinetic and equilibrium studies at pH 3.0±0.05 in anoxic media coupled with spectroscopic and microscopic examinations at variable conditions to understand possible As(III) binding mechanisms and the redox stability of As(III) on schwertmannite, a prominent ferric mineral in acid mine drainage environments. Schwertmannite acted as an efficient scavenger for As(III) compared to goethite at identical sorbent:solute ratios. As K-edge X-ray absorption near-edge structure (XANES) demonstrated partial oxidation of sorbed As(III) to As(V) on both the minerals depending on the Fe(III)/As(III) ratios (goethite acted as a better oxidant than schwertmannite). Sorbed As(III) and As(V) coordinated in a bidentate binuclear binding mechanism with As(III)/As(V)-O and As(III)/As(V)-Fe interatomic distances as 1.78/1.69 and 3.37/3.31Å, respectively. Scanning (SEM-EDX) and transmission (TEM) electron microscopic, and IR spectroscopic measurements revealed the formation of As-containing surface coatings by sorbed As on schwertmannite. PMID:24361800

  7. Spectroscopic characterization of bioactive carboxyamide with trinuclear lanthanide (III) ions.

    PubMed

    Singh, Bibhesh K; Prakash, Anant; Adhikari, Devjani

    2009-10-15

    Complexes of La(III), Sm(III), Eu(III) and Tb(III) with bioactive carboxyamide ligands N',N''-bis(3-caboxy-1-oxophenelenyl)2-amino-N-arylbenzamidine have been synthesized and characterized by various physico-chemical techniques. Mass spectrum explains the successive degradation of the molecular species in solution and justifies ML complexes. Vibrational spectra indicate coordination of Ln(III) with amide and carboxylate oxygen of the ligand along with nitrate ions. The magnetic moment of Sm(III) and Eu(III) complexes showed slightly higher-values which originated due to low J-J separation leading to thermal population of next higher energy J levels and susceptibility due to first order Zeeman effect. The strong luminescence emitting peaks at 587 nm for Eu(III) and 543 nm for Tb(III) can be observed, which could be attributed to the ligand have an enhanced effect to the luminescence intensity of the Eu(III) and Tb(III). The thermal behaviour of complexes shows that water molecules and nitrate ion are removed in first step followed by the removal of two molecules of nitrate ions and then decomposition of the ligand molecule in subsequent step. Kinetic and thermodynamic parameters were computed from the thermal data using Coats and Redferm method, which confirm first order kinetics. PMID:19716336

  8. A deficiency of O III in the Io plasma torus

    NASA Technical Reports Server (NTRS)

    Brown, R. A.; Shemansky, D. E.; Johnson, R. E.

    1983-01-01

    Evidence for a deficiency of O III ions in the Io plasma torus is reported and implications of this deficiency for the physical processes controlling the plasma are considered. Observations of the O III 5007-A as well as Cl III and S III emissions from the Io plasma torus were made by a ground-based echelle spectrograph and intensified Reticon detector in February and May, 1981. The O III observations allow an upper limit of 4/cu cm to be placed on torus O III abundance, which is inconsistent with expectations for a low density plasma controlled by electron collisions. The inclusion of ion-ion and ion-atom charge exchange reactions and a depleted high energy electron component in the model is found to suppress O III levels, however observed limiting values are only achieved if it is assumed that the O III is kinetically hot. In addition, the charge-exchange model developed is inconsistent with previous observations of the kinetics of the S II-S III system. The present observations also establish upper limits of 2 R on 5518-A and 5538-A Cl III emission, and an emission rate of 58 + or - 40 R for the S III 6312-A line in the hot torus.

  9. Selectivity of azine ligands toward lanthanide(III)/actinide(III) differentiation: a relativistic DFT based rationalization.

    PubMed

    Zaiter, Abdellah; Amine, Boudersa; Bouzidi, Yamina; Belkhiri, Lotfi; Boucekkine, Abdou; Ephritikhine, Michel

    2014-05-01

    Polyazines emerge as highly selective ligands toward actinide versus lanthanide separation. Electronic structures of several mono- and polyazine f-complexes of general formula MX3L (M(+3) = Ce, Nd, Eu, U, Am, and Cm; X = RCp(-) or NO3(-); L = N-donor ligand) related to Ln(III)/An(III) differentiation have been investigated using scalar relativistic ZORA/DFT calculations. In all cases, DFT calculations predict shorter An-N bonds than Ln-N ones whatever the azine used, in good agreement with available experimental data. The An-N bonds are also characterized by higher stretching frequencies than Ln-N bonds. The electronic structures of all species have been studied using different population analyses, among them natural population (NPA) and the quantum theory of atoms in molecule approach (QTAIM), as well as using different bond indices. The ability for Ln(III)/An(III) differentiation of the terdentate bipyrazolate BPPR ligand in the M(BPPR)(NO3)3 complexes (M(3+) = Ce, Eu, U and Am ; R = H, 2,2-dimethylpropyl) where BPP = 2,6-bis(dialkyl-1H-pyrazol-3-yl)pyridine has been studied, with a special emphasis on the total metal-ligand bonding energy (TBE). The ZORA/DFT approach was found to properly reproduce the higher selectivity of the polyazine BPP ligand compared to monoazines, especially for the Eu(III)/Am(III) pair operating in spent nuclear fuel, using computed TBEs as criterion. Moreover, the orbital part of the total bonding energy appears also to rationalize well the observed selectivity. PMID:24749559

  10. 9,10-phenanthrenesemiquinone radical complexes of ruthenium(III), osmium(III) and rhodium(III) and redox series.

    PubMed

    Biswas, Manas Kumar; Patra, Sarat Chandra; Maity, Amarendra Nath; Ke, Shyue-Chu; Weyhermüller, Thomas; Ghosh, Prasanta

    2013-05-14

    Reactions of 9,10-phenanthrenequinone (PQ) in toluene with [M(II)(PPh3)3X2] at 298 K afford green complexes, trans-[M(PQ)(PPh3)2X2] (M = Ru, X = Cl, 1; M = Os, X = Br, 2) in moderate yields. Reaction of anhydrous RhCl3 with PQ and PPh3 in boiling ethanol affords the dark brown paramagnetic complex, cis-[Rh(PQ)(PPh3)2Cl2] (3) in good yields. Diffusion of iodine solution in n-hexane to the trans-[Os(PQ) (PPh3)2(CO)(Br)] solution in CH2Cl2 generates the crystals of trans-[Os(PQ)(PPh3)2(CO)(Br)](+)I3(-), (4(+))I3(-)), in lower yields. Single crystal X-ray structure determinations of 1·2toluene, 2·CH2Cl2 and 4(+)I3(-), UV-vis/NIR absorption spectra, EPR spectra of 3, electrochemical activities and DFT calculations on 1, 2, trans-[Ru(PQ)(PMe3)2Cl2] (1Me), trans-[Os(PQ)(PMe3)2Br2] (2Me), cis-[Rh(PQ)(PMe3)2Cl2] (3Me) and their oxidized and reduced analogues including trans-[Os(PQ)(PMe3)2(CO)(Br)](+) (4Me(+)) substantiated that 1-3 are the 9,10-phenanthrenesemiquinone radical (PQ(˙-)) complexes of ruthenium(III), osmium(III) and rhodium(III) and are defined as trans/cis-[M(III)(PQ(˙-))(PPh3)2X2] with a minor contribution of the resonance form trans/cis-[M(II)(PQ)(PPh3)2X2]. Two comparatively longer C-O (1.286(4) Å) and the shorter C-C lengths (1.415(7) Å) of the OO-chelate of 1·2toluene and 2·CH2Cl2 and the isotropic fluid solution EPR signal at g = 1.999 of 3 are consistent with the existence of the reduced PQ(˙-) ligand in 1-3 complexes. Anisotropic EPR spectra of the frozen glasses (g11 = g22 = 2.0046 and g33 = 1.9874) and solids (g11 = g22 = 2.005 and g33 = 1.987) instigate the contribution of the resonance form, cis-[Rh(II)(PQ)(PPh3)2Cl2] in 3. DFT calculations established that the closed shell singlet (CSS) solutions of 1Me and 2Me are unstable due to open shell singlet (OSS) perturbation. However, the broken symmetry (BS) (1,1) Ms = 0 solutions of 1Me and 2Me are respectively 22.6 and 24.2 kJ mole(-1) lower in energy and reproduced the experimental bond

  11. Unequivocal synthetic pathway to heterodinuclear (4f,4f') complexes: magnetic study of relevant (Ln(III), Gd(III)) and (Gd(III), Ln(III)) complexes.

    PubMed

    Costes, Jean-Pierre; Nicodème, Franck

    2002-08-01

    The tripodal ligand tris[4-(2-hydroxy-3-methoxyphenyl)-3-aza-3-buten]amine (LH(3)) is capable of coordinating to two different lanthanide ions to give complexes formulated as [LLnLn'(NO(3))(3)].x H(2)O. The stepwise synthetic procedure consists of introducing first a Ln(III) ion in the inner N(4)O(3) coordination site. The isolated neutral complex LLn is then allowed to react with a second and different Ln' ion that occupies the outer O(6) site, thus yielding a [LLnLn'(NO(3))(3)].x H(2)O complex. A FAB(+) study has confirmed the existence of (Ln, Ln') entities as genuine, when the Ln' ion in the outer site has a larger ionic radius than the Ln ion in the inner site. The qualitative magnetic study of the (Gd, Ln) and (Ln, Gd) complexes, based on the comparison of the magnetic properties of (Gd, Ln) (or (Ln, Gd)) pairs and (Y, Ln) (or (Ln, La)) pairs, is very informative. Indeed, these former complexes are governed by the thermal population of the Ln(III) Stark levels and the Ln-Gd interaction, while the latter are influenced by the thermal population of the Ln(III) Stark levels. We have been able to show that a ferromagnetic interaction exists at low temperature in the (Gd, Nd), (Gd, Ce), and (Yb, Gd) complexes. In contrast, an antiferromagnetic interaction occurs in the (Dy, Gd) and (Er, Gd) complexes. Although we cannot give a quantitative value to these interactions, we can affirm that their magnitudes are weak since they are only perceptible at very low temperature. PMID:12203324

  12. Space Processing Applications Rocket project SPAR III

    NASA Technical Reports Server (NTRS)

    Reeves, F.

    1978-01-01

    This document presented the engineering report and science payload III test report and summarized the experiment objectives, design/operational concepts, and final results of each of five scientific experiments conducted during the third Space Processing Applications Rocket (SPAR) flight flown by NASA in December 1976. The five individual SPAR experiments, covering a wide and varied range of scientific materials processing objectives, were entitled: Liquid Mixing, Interaction of Bubbles with Solidification Interfaces, Epitaxial Growth of Single Crystal Film, Containerless Processing of Beryllium, and Contact and Coalescence of Viscous Bodies.

  13. Have We Finally Found Pop III Stars?

    NASA Astrophysics Data System (ADS)

    Kohler, Susanna

    2015-08-01

    Elusive Population: Population III stars — the theoretical generation of extremely metal-poor stars that should have been formed in the early universe before metals existed — have been conspicuously absent in observations. But a team led by David Sobral (Institute of Astrophysics and Space Sciences, University of Lisbon, and Leiden Observatory) may have changed this paradigm with their recent detection of an extremely bright galaxy in the early universe. The team's broad survey of distant galaxies using ESO's Very Large Telescope provides a glimpse of the universe as it was only 800 million years after the Big Bang. The survey uncovered several unusually bright galaxies — including the brightest galaxy ever seen at this distance, an important discovery by itself. But further scrutiny of this galaxy, named CR7, produced an even more exciting find: a bright pocket of the galaxy contained no sign of any metals. Follow-up with other telescopes confirmed this initial detection. Formation Waves: Sobral and his team postulate that we are observing this galaxy at just the right time to have caught a cluster of Population III stars — the bright, metal-free region of the galaxy — at the end of a wave of early star formation. The observations of CR7 also suggest the presence of regular stars in clumps around the metal-free pocket. These older, surrounding clusters may have formed stars first, helping to ionize a local bubble in the galaxy and allowing us to now observe the light from CR7. It was previously thought that Population III stars might only be found in small, dim galaxies, making them impossible for us to detect. But CR7 provides an interesting alternative: this galaxy is bright, and the candidate Population III stars are surrounded by clusters of normal stars. This suggests that these first-generation stars might in fact be easier to detect than was originally thought. Additional follow-up observations with other telescopes will help to confirm the

  14. Diluted magnetic III-V semiconductors

    NASA Astrophysics Data System (ADS)

    Munekata, H.; Ohno, H.; von Molnar, S.; Segmüller, Armin; Chang, L. L.; Esaki, L.

    1989-10-01

    A new diluted magnetic III-V semiconductor of In1-xMnxAs (x<=0.18) has been produced by molecular-beam epitaxy. Films grown at 300 °C are predominantly ferromagnetic and their properties suggest the presence of MnAs clusters. Films grown 200 °C, however, are predominantly paramagnetic, and the lattice constant decreases with increasing Mn composition; both are indicative of the formation of a homogeneous alloy. These films have n-type conductivity and reduced band gaps.

  15. The III-Bi binary compounds

    NASA Astrophysics Data System (ADS)

    Keen, Benjamin

    Bismuth containing III-V alloys such as GaAsBi, GaSbBi, InSbBi and InAsBi have recently become of great interest in the development of optical devices in the infrared spectrum. Difficulties in fabricating these materials stems, in part, from the lack of experimental data on the characteristics of the III-Bi family of compounds: AlBi, GaBi, and InBi. This thesis outlines the growth conditions and characteristics of the MBE deposition of InBi. To date, InBi remains the only one of the three compounds that has been experimentally reported, and the difficulties associated with the growth of AlBi and GaBi are also described herein. InBi thin films were grown on GaAs substrates at temperatures ranging from 50 °C to 100 °C. Unlike other III-V materials, which require a group V overpressure during deposition, to achieve stoichiometric quantities of indium and bismuth an In:Bi BEP ratio of 4:3 was found to be necessary. InBi samples were studied by a variety of measurement techniques, including SEM, EDX, XRD, HAXPES, and HRTEM. Films were found to grow in a 3-D Volmer-Weber mode, forming hemispherical droplets on the substrate surface. These droplets indicated clear signs of Ostwald ripening during growth, but maintained their distribution after deposition. InBi samples are believed to be semi-metallic, confirming some of the properties predicted by density functional theory (DFT) calculations. However, analysis of the crystal structure at the substrate/droplet interface indicates the epitaxial growth of InBi is in the zinc-blende configuration, instead of the PbO configuration, in direct opposition to predictions by DFT and experimental data reported from bulk crystal studies. Attempts to grow the other III-Bi materials, GaBi and AlBi, by MBE also resulted in 3-D droplet formation, but both gallium and aluminum failed to incorporate with bismuth to form a compound. Instead, the materials formed segregated regions in the droplets, clearly visible to SEM and EDX

  16. Prototype studies for the CLEO III RICH

    SciTech Connect

    Kopp, S.; Artuso, M.; Efimov, A.; Gao, M.; Playfer, S.; Mountain, R.; Muheim, F.; Stone, S.

    1996-06-01

    The authors describe a prototype RICH detector that has been built as part of design work for the CLEO III RICH. Cherenkov photons are produced in a LiF radiator, and are detected in a multiwire chamber with a CaF{sub 2} entrance window containing a gas mixture of methane and TEA. Signals are read out from 2016 cathode pads using low noise Viking chips. First results from this prototype show a yield of 13 photoelectrons per image in agreement with the design studies.

  17. III Astronomy School: the world of stars

    NASA Astrophysics Data System (ADS)

    Valdivielso, L.

    2015-05-01

    The Astronomy School is an activity organized by the Centro de Estudios de Física del Cosmos de Aragón. It is celebrated every year at the end of the summer or the beginning of autumn with the purpose of bringing Astronomy and Astrophysics to the population from an educational and a training point of view. Its III Edition, held in October 2013, has been financed by the Spanish Astronomical Society and was focused on stellar physics. This contribution describes the School and the results.

  18. Arthroscopic fixation of type III acromioclavicular dislocations.

    PubMed

    Somers, Jan F A; Van der Linden, Dietert

    2007-10-01

    Type III Acromio-Clavicular Joint dislocations can be treated successfully by surgical stabilisation in situ, with or without reconstruction of the coracoclavicular ligaments. The authors describe a simple and reliable mode of fixation, performed arthroscopically. The technique can be used for in situ fixation, or as part of an arthroscopically assisted Weaver and Dunn procedure. Using a metallic anchor loaded with a braided polyfilament suture, a strong and reliable fixation of the clavicle to the coracoid process is obtained. No hardware removal is necessary. Concomitant glenohumeral pathology can be treated simultaneously. PMID:18019910

  19. Proteome of Geobacter sulfurreducens grown with Fe(III) oxide or Fe(III) citrate as the electron acceptor.

    SciTech Connect

    Ding, Y-H R.; Hixson, Kim K.; Aklujkar, Ma; Lipton, Mary S.; Smith, Richard D.; Lovley, Derek R.; Mester, Tunde

    2008-12-01

    e(III) oxides are the most abundant source of reducible Fe(III) by microorganisms in most soils and sediments, yet few studies on the physiology of Fe(III)-reducing microorganisms during growth on Fe(III) oxide have been conducted because of the technical difficulties in working with cell growth and harvest in the presence of Fe(III) oxides. Geobacter sulfurreducens is a representative of the Geobacter species that predominate in a variety of subsurface environments in which Fe(III) oxide is important. In order to better understand the physiology of Geobacter species during growth on Fe(III) oxide, the proteome of G. sulfurreducens grown on Fe(III) oxide was compared with the proteome of cells grown with soluble Fe(III) citrate. Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) revealed 19 proteins that were more abundant during growth on Fe(III) oxide than on soluble Fe(III). These included proteins related to protein synthesis, electron transfer and energy production, oxidative stress, protein folding, outer membrane proteins, nitrogen metabolism and hypothetical proteins. Further analysis of the proteome with the accurate mass and time (AMT) tag method revealed additional proteins associated with growth on Fe(III) oxide. These included the outer-membrane c-type cytochrome, OmcS and OmcG, which genetic studies have suggested are required for Fe(III) oxide reduction. Furthermore, several other cytochromes, as yet unstudied, were detected to be significantly up regulated during growth on Fe(III) oxide and other proteins of unknown function were more abundant during growth on Fe(III) oxide than on soluble Fe(III). PilA, the structural protein for pili, which is required for Fe(III) oxide reduction, and other pilin-associated proteins were also more abundant during growth on Fe(III) oxide. Confirmation of the differential expression of proteins known to be important in Fe(III) oxide reduction was observed, and an additional number of previously

  20. Defining the RNA polymerase III transcriptome: Genome-wide localization of the RNA polymerase III transcription machinery in human cells

    PubMed Central

    Canella, Donatella; Praz, Viviane; Reina, Jaime H.; Cousin, Pascal; Hernandez, Nouria

    2010-01-01

    Our view of the RNA polymerase III (Pol III) transcription machinery in mammalian cells arises mostly from studies of the RN5S (5S) gene, the Ad2 VAI gene, and the RNU6 (U6) gene, as paradigms for genes with type 1, 2, and 3 promoters. Recruitment of Pol III onto these genes requires prior binding of well-characterized transcription factors. Technical limitations in dealing with repeated genomic units, typically found at mammalian Pol III genes, have so far hampered genome-wide studies of the Pol III transcription machinery and transcriptome. We have localized, genome-wide, Pol III and some of its transcription factors. Our results reveal broad usage of the known Pol III transcription machinery and define a minimal Pol III transcriptome in dividing IMR90hTert fibroblasts. This transcriptome consists of some 500 actively transcribed genes including a few dozen candidate novel genes, of which we confirmed nine as Pol III transcription units by additional methods. It does not contain any of the microRNA genes previously described as transcribed by Pol III, but reveals two other microRNA genes, MIR886 (hsa-mir-886) and MIR1975 (RNY5, hY5, hsa-mir-1975), which are genuine Pol III transcription units. PMID:20413673

  1. White Light Emissive Dy(III) Single-Molecule Magnets Sensitized by Diamagnetic [Co(III) (CN)6 ](3-) Linkers.

    PubMed

    Chorazy, Szymon; Rams, Michał; Nakabayashi, Koji; Sieklucka, Barbara; Ohkoshi, Shin-Ichi

    2016-05-23

    The self-assembly of Dy(III) -3-hydroxypyridine (3-OHpy) complexes with hexacyanidocobaltate(III) anions in water produces cyanido-bridged {[Dy(III) (3-OHpy)2 (H2 O)4 ] [Co(III) (CN)6 ]}⋅H2 O (1) chains. They reveal a single-molecule magnet (SMM) behavior with a large zero direct current (dc) field energy barrier, ΔE=266(12) cm(-1) (≈385 K), originating from the single-ion property of eight-coordinated Dy(III) of an elongated dodecahedral geometry, which are embedded with diamagnetic [Co(III) (CN)6 ](3-) ions into zig-zag coordination chains. The SMM character is enhanced by the external dc magnetic field, which results in the ΔE of 320(23) cm(-1) (≈460 K) at Hdc =1 kOe, and the opening of a butterfly hysteresis loop below 6 K. Complex 1 exhibits white Dy(III) -based emission realized by energy transfer from Co(III) and 3-OHpy to Dy(III) . Low temperature emission spectra were correlated with SMM property giving the estimation of the zero field ΔE. 1 is a unique example of bifunctional magneto-luminescent material combining white emission and slow magnetic relaxation with a large energy barrier, both controlled by rich structural and electronic interplay between Dy(III) , 3-OHpy, and [Co(III) (CN)6 ](3-) . PMID:26990787

  2. The influence of triplet energy levels of bridging ligands on energy transfer processes in Ir(III)/Eu(III) dyads.

    PubMed

    Jiang, Weili; Lou, Bin; Wang, Jianqiang; Lv, Hongbin; Bian, Zuqiang; Huang, Chunhui

    2011-11-21

    A series of N^N,O^O-bridging ligands based on substituted 1-(pyridin-2-yl)-3-methyl-5-pyrazolone and their corresponding heteroleptic iridium(III) complexes as well as Ir-Eu bimetallic complexes were synthesized and fully characterized. The influence of the triplet energy levels of the bridging ligands on the energy transfer (ET) process from the Ir(III) complexes to Eu(III) ions in solution was investigated at 77 K in Ir(III)/Eu(III) dyads. Photophysical experiment results show the bridging ligands play an important role in the ET process. Only when the triplet energy level of the bridging ligand was lower than the triplet metal-to-ligand charge transfer ((3)MLCT) energy level of the Ir moiety, was pure emission from the Eu(III) ion observed, implying complete ET took place from the Ir moiety to the Eu(III) ion. PMID:21931913

  3. Extraction Based on in situ Formation of Dithiocarbamate for Separation of Am(III) from Ln(III)

    SciTech Connect

    Miyashita, Sunao; Yanaga, Makoto; Okuno, Kenji; Suganuma, Hideo; Satoh, Isamu

    2007-07-01

    A new solvent extraction technique based on in situ extractant formation of dithiocarbamate derivatives was constructed for the purpose of separation of Am(III) from Ln(III). Ammonium salts of dithiocarbamate in this technique are formed during the extraction course by the reaction between secondary amines and carbon disulfide in organic phase. The effects of substituent of secondary amines against the behavior of in situ formation of dithiocarbamate and the distribution behaviors of Am(III) and Ln(III)(especially Eu(III)) into nitrobenzene phase using in situ formation of dithiocarbamate were investigated. It was revealed that amines containing substituent in {alpha} position of amine were not suited that for in situ extractant formation method. The values of separation factor of Am(III)/Eu(III) >10{sup 4} were obtained by the new method using five di-substituted amines/CS{sub 2}/nitrobenzene system. (authors)

  4. Sources of type III solar microwave bursts

    NASA Astrophysics Data System (ADS)

    Zhdanov, Dmitriy; Lesovoi, Sergey; Tokhchukova, Susanna

    2016-06-01

    Microwave fine structures allow us to study plasma evolution in an energy release region. The Siberian Solar Radio Telescope (SSRT) is a unique instrument designed to examine fine structures at 5.7 GHz. A complex analysis of data from RATAN-600, 4-8 GHz spectropolarimeter, and SSRT, simultaneously with extreme UV data, made it possible to localize sources of III type microwave drift bursts in August 10, 2011 event within the entire frequency band of burst occurrences, as well as to determine the most probable region of primary energy release. To localize sources of III type bursts from RATAN-600 data, an original method for data processing has been worked out. At 5.7 GHz, the source of bursts was determined along two coordinates whereas at 4.5, 4.7, 4.9, 5.1, 5.3, 5.5 and 6.0 GHz, their locations were identified along one coordinate. The size of the burst source at 5.1 GHz was found to be maximum as compared to source sizes at other frequencies.

  5. Radioactivity backgrounds in ZEPLIN-III

    NASA Astrophysics Data System (ADS)

    Araújo, H. M.; Akimov, D. Yu.; Barnes, E. J.; Belov, V. A.; Bewick, A.; Burenkov, A. A.; Chepel, V.; Currie, A.; Deviveiros, L.; Edwards, B.; Ghag, C.; Hollingsworth, A.; Horn, M.; Kalmus, G. E.; Kobyakin, A. S.; Kovalenko, A. G.; Lebedenko, V. N.; Lindote, A.; Lopes, M. I.; Lüscher, R.; Majewski, P.; Murphy, A. St. J.; Neves, F.; Paling, S. M.; Pinto da Cunha, J.; Preece, R.; Quenby, J. J.; Reichhart, L.; Scovell, P. R.; Silva, C.; Solovov, V. N.; Smith, N. J. T.; Smith, P. F.; Stekhanov, V. N.; Sumner, T. J.; Thorne, C.; Walker, R. J.

    2012-03-01

    We examine electron and nuclear recoil backgrounds from radioactivity in the ZEPLIN-III dark matter experiment at Boulby. The rate of low-energy electron recoils in the liquid xenon WIMP target is 0.75 ± 0.05 events/kg/day/keV, which represents a 20-fold improvement over the rate observed during the first science run. Energy and spatial distributions agree with those predicted by component-level Monte Carlo simulations propagating the effects of the radiological contamination measured for materials employed in the experiment. Neutron elastic scattering is predicted to yield 3.05 ± 0.5 nuclear recoils with energy 5-50 keV per year, which translates to an expectation of 0.4 events in a 1 yr dataset in anti-coincidence with the veto detector for realistic signal acceptance. Less obvious background sources are discussed, especially in the context of future experiments. These include contamination of scintillation pulses with Cherenkov light from Compton electrons and from β activity internal to photomultipliers, which can increase the size and lower the apparent time constant of the scintillation response. Another challenge is posed by multiple-scatter γ-rays with one or more vertices in regions that yield no ionisation. If the discrimination power achieved in the first run can be replicated, ZEPLIN-III should reach a sensitivity of ˜1 × 10-8pb · yr to the scalar WIMP-nucleon elastic cross-section, as originally conceived.

  6. Sloan Digital Sky Survey III (SDSS-III), Data Release 8

    DOE Data Explorer

    Building on the legacy of the Sloan Digital Sky Survey (SDSS) and SDSS-II, the SDSS-III Collaboration is working to map the Milky Way, search for extrasolar planets, and solve the mystery of dark energy. SDSS-III's first release, Data Release 8 (DR8), became available in the first half of 2012. DR8 contains all the images ever taken by the SDSS telescope. Together, these images make up the largest color image of the sky ever made. A version of the DR8 image is shown to the right. DR8 also includes measurements for nearly 500 million stars, galaxies, and quasars, and spectra for nearly two million. All of DR8's images, spectra, and measurements are available to anyone online. You can browse through sky images, look up data for individual objects, or search for objects anywhere using any criteria. SDSS-III will collect data from 2008 to 2014, using the 2.5-meter telescope at Apache Point Observatory. SDSS-III consists of four surveys, each focused on a different scientific theme. These four surveys are: 1) Baryon Oscillation Spectroscopic Survey (BOSS); 2) SEGUE-2 (Sloan Extension for Galactic Understanding and Exploration); 3) The APO Galactic Evolution Experiment (APOGEE); and 4) The Multi-object APO Radial Velocity Exoplanet Large-area Survey (MARVELS). [Copied with edits from http://www.sdss3.org/index.php

  7. Lanthanum(III) and praseodymium(III) complexes with isatin thiosemicarbazones

    NASA Astrophysics Data System (ADS)

    Rai, Anita; Sengupta, Soumitra K.; Pandey, Om P.

    2005-09-01

    Ten new lanthanum(III) and praseodymium(III) complexes of the general formula Na[La(L) 2H 2O] (Ln = La(III) or Pr(III); LH 2 = thiosemicarbazones) derived from the condensation of isatin with 4-phenyl thiosemicarbazide, 4-(4-chlorophenyl) thiosemicarbazide, 4-(2-nitrophenyl) thiosemicarbazide, 4-(2-bromophenyl) thiosemicarbazide and 4-(2-methylphenyl) thiosemicarbazide, have been synthesized in methanol in presence of sodium hydroxide. The XRD spectra of the complexes were monitored to verify complex formation. The complexes have also been characterized by elemental analysis, molar conductance, electronic absorption and fluorescence, infrared, far infrared, 1H and 13C NMR spectral studies. Thermal studies of these complexes have been carried out in the temperature range 25-800 °C using TG, DTG and DTA techniques. All these complexes decompose gradually with the formation of Ln 2O 3 as the end product. The Judd-ofelt intensity parameter, oscillator strength, transition probability, stimulated emission cross section for different transitions of Pr 3+ for 4-phenyl thiosemicarbazones have been calculated.

  8. Electrochemistry and spectroscopy of ortho-metalated complexes of Ir(III) and Rh(III)

    SciTech Connect

    Ohsawa, Y.; Sprouse, S.; King, K.A.; DeArmond, M.K.; Hanck, K.W.; Watts, R.J.

    1987-02-26

    The electrochemical and UV-visible spectroscopic properties of Rh(III) and Ir(III) complexes of the ortho-metalating (NC) ligands, 2-phenylpyridine (ppy) and benzo(h)quinone (bzq), have been studied. Cyclic voltammetric studies of several of the dimeric species, (M(NC)/sub 2/Cl)/sub 2/, indicate metal-centered oxidation occurs at moderate potentials. Cationic monomers of the type M(NC)/sub 2/(NN)/sup +/ where (NN) = 2,2'-bipyridine or 1,10-phenanthroline have been prepared by reaction of the chelating ligands with the parent dimers. Cyclic voltammetric studies of these monomers indicate that several reversible ligand-centered reductions are generally observed and that the chelating ligand is more easily reduced than is the ortho-metalating ligand. Spectroscopic studies of the mixed ligand monomers indicate that dual emissions from MLCT states associated with the ortho-metalating and chelating ligands occur in the Ir(III) complexes whereas a single emission from a ligand-localized excited state is observed in the Rh(III) complexes. These results are discussed in terms of electronic and nuclear coupling factors analogous to those encountered in descriptions of bimolecular energy and electron-transfer processes.

  9. Stratospheric Aerosol and Gas Experiment III on the International Space Station (SAGE III/ISS)

    NASA Technical Reports Server (NTRS)

    Gasbarre, Joseph; Walker, Richard; Cisewski, Michael; Zawodny, Joseph; Cheek, Dianne; Thornton, Brooke

    2015-01-01

    The Stratospheric Aerosol and Gas Experiment III on the International Space Station (SAGE III/ISS) mission will extend the SAGE data record from the ideal vantage point of the International Space Station (ISS). The ISS orbital inclination is ideal for SAGE measurements providing coverage between 70 deg north and 70 deg south latitude. The SAGE data record includes an extensively validated data set including aerosol optical depth data dating to the Stratospheric Aerosol Measurement (SAM) experiments in 1975 and 1978 and stratospheric ozone profile data dating to the Stratospheric Aerosol and Gas Experiment (SAGE) in 1979. These and subsequent data records, notably from the SAGE II experiment launched on the Earth Radiation Budget Satellite in 1984 and the SAGE III experiment launched on the Russian Meteor-3M satellite in 2001, have supported a robust, long-term assessment of key atmospheric constituents. These scientific measurements provide the basis for the analysis of five of the nine critical constituents (aerosols, ozone (O3), nitrogen dioxide (NO2), water vapor (H2O), and air density using O2) identified in the U.S. National Plan for Stratospheric Monitoring. SAGE III on ISS was originally scheduled to fly on the ISS in the same timeframe as the Meteor-3M mission, but was postponed due to delays in ISS construction. The project was re-established in 2009.

  10. Apoprotein C-III: A review of its clinical implications.

    PubMed

    Jin, Jing-Lu; Guo, Yuan-Lin; Li, Jian-Jun

    2016-09-01

    Apoprotein C-III (apoC-III), originating from the apoA-I/C-III/A-IV gene cluster affected by multiple regulating factors, has been demonstrated to have a validated link with hypertriglyceridemia in humans. Following genome studies establishing the impact of apoC-III on both plasma triglyceride (TG) level and cardiovascular disease (CVD), apoC-III offers us a novel explanation attempting to resolve the long-existing confusion with regard to the atherogenic effect of TG. Notably, apoC-III exerts its atherogenic effect by means of not only intervening in the function and metabolism of various lipid molecules, but also accelerating pro-inflammatory effects between monocytes and endothelial cells. Data have suggested that diabetes, a common endocrine disease, also correlates closely with apoC-III in its apoptosis process of islet βcells. In fact, apoC-III genes, with various mutations among individuals, are also found to have relevance to other diseases, including fatty liver disease. Fortunately, besides present day therapeutic strategies, such as lifestyle changes and lipid-lowering drug treatments, a promising new antisense drug specifically targeting on apoC-III gene expression opens up new avenues. This article mainly summarizes the clinical implication of apoC-III and its future directions of treatment. PMID:27318213

  11. Molecular magnets based on homometallic hexanuclear lanthanide(III) complexes.

    PubMed

    Das, Sourav; Hossain, Sakiat; Dey, Atanu; Biswas, Sourav; Sutter, Jean-Pascal; Chandrasekhar, Vadapalli

    2014-05-19

    The reaction of lanthanide(III) chloride salts (Gd(III), Dy(III), Tb(III), and Ho(III)) with the hetero donor chelating ligand N'-(2-hydroxy-3-methoxybenzylidene)-6-(hydroxymethyl)picolinohydrazide (LH3) in the presence of triethylamine afforded the hexanuclear Ln(III) complexes [{Ln6(L)2(LH)2}(μ3-OH)4][MeOH]p[H2O]q[Cl]4·xH2O·yCH3OH (1, Ln = Gd(III), p = 4, q = 4, x = 8, y = 2; 2, Ln = Dy(III), p = 2, q = 6, x = 8, y = 4; 3, Ln = Tb(III), p = 2, q = 6, x = 10, y = 4; 4, Ln = Ho(III), p = 2, q = 6, x = 10, y = 2). X-ray diffraction studies revealed that these compounds possess a hexanuclear [Ln6(OH)4](14+) core consisting of four fused [Ln3(OH)](8+) subunits. Both static (dc) and dynamic (ac) magnetic properties of 1-4 have been studied. Single-molecule magnetic behavior has been observed in compound 2 with an effective energy barrier and relaxation time pre-exponential parameters of Δ/kB = 46.2 K and τ0 = 2.85 × 10(-7) s, respectively. PMID:24766539

  12. Overview of the Stratospheric Aerosol and Gas Experiment III (SAGE III) on the International Space Station

    NASA Astrophysics Data System (ADS)

    Flittner, David; Pitts, Michael; Zawodny, Joe; Hill, Charles; Damadeo, Robert; Moore, Randy; Cisewski, Michael

    2012-07-01

    The Stratospheric Aerosol and Gas Experiment (SAGE) III is the fourth generation of solar occultation instruments operated by NASA, the first coming under a different acronym, to investigate the Earth's upper atmosphere. Three flight-ready SAGE III instruments were built by Ball Aerospace in the late 1990s, with one launched aboard the former Russian Avaiation and Space Agency (now known as Roskosmos) Meteor-3M (M3M) platform on 10 December 2001 (continuing until the platform lost power in 2006). Another of the original instruments was manifested for the International Space Station (ISS) in the 2004 time frame, but was delayed because of budgetary considerations. Fortunately, that SAGE III/ISS mission was restarted in 2009 with a major focus upon filling an anticipated gap in ozone and aerosol observations in the second half of this decade. This exciting mission utilizes contributions from both the Science Mission Directorate and the Human Exploration and Operations Mission Directorate within the National Aeronautics and Space Administration and the European Space Agency to enable scientific measurements that will provide the basis for the analysis of five of the nine critical constituents identified in the U.S. National Plan for Stratospheric Monitoring. A related paper by Anderson et al. discusses the. Presented here is an overview of the mission architecture, its implementation and the data that will be produced by SAGE III/ISS, including their expected accuracy and coverage. The 52-degree inclined orbit of the ISS is well-suited for solar occultation and provides near-global observations on a monthly basis with excellent coverage of low and mid-latitudes. This is similar to that of the SAGE II mission (1985-2005), whose data set has served the international atmospheric science community as a standard for stratospheric ozone and aerosol measurements. The nominal science products include vertical profiles of trace gases, such as ozone, nitrogen dioxide and water

  13. Synthesis, characterization and stability of Cr(III) and Fe(III) hydroxides.

    PubMed

    Papassiopi, N; Vaxevanidou, K; Christou, C; Karagianni, E; Antipas, G S E

    2014-01-15

    Chromium is a common contaminant of soils and aquifers and constitutes a major environmental problem. In nature, chromium usually exists in the form of two oxidation states, trivalent, Cr(III), which is relatively innocuous for biota and for the aquatic environment, and hexavalent, Cr(VI) which is toxic, carcinogenic and very soluble. Accordingly, the majority of wastewater and groundwater treatment technologies, include a stage where Cr(VI) is reduced to Cr(III), in order to remove chromium from the aqueous phase and bind the element in the form of environmentally stable solid compounds. In the absence of iron the final product is typically of the form Cr(OH)3·xH2O whereas in the presence of iron the precipitate is a mixed Fe(1-x)Crx(OH)3 phase. In this study, we report on the synthesis, characterisation and stability of mixed (Fex,Cr1-x)(OH)3 hydroxides as compared to the stability of Cr(OH)3. We established that the plain Cr(III) hydroxide, abiding to the approximate molecular formula Cr(OH)3·3H2O, was crystalline, highly soluble, i.e. unstable, with a tendency to transform into the stable amorphous hydroxide Cr(OH)3(am) phase. Mixed Fe0.75Cr0.25(OH)3 hydroxides were found to be of the ferrihydrite structure, Fe(OH)3, and we correlated their solubility to that of a solid solution formed by plain ferrihydrite and the amorphous Cr(III) hydroxide. Both our experimental results and thermodynamic calculations indicated that mixed Fe(III)-Cr(III) hydroxides are more effective enhancers of groundwater quality, in comparison to the plain amorphous or crystalline Cr(III) hydroxides, the latter found to have a solubility typically higher than 50μg/l (maximum EU permitted Cr level in drinking water), while the amorphous Cr(OH)3(am) phase was within the drinking water threshold in the range 5.7

  14. Synthesis of Imine-Naphthol Tripodal Ligand and Study of Its Coordination Behaviour towards Fe(III), Al(III), and Cr(III) Metal Ions

    PubMed Central

    Kaur, Kirandeep

    2014-01-01

    A hexadentate Schiff base tripodal ligand is synthesized by the condensation of tris (2-aminoethyl) amine with 2-hydroxy-1-naphthaldehyde and characterized by various spectroscopic techniques like UV-VIS, IR, NMR, MASS, and elemental analysis. The solution studies by potentiometric and spectrophotometric methods are done at 25 ± 1°C, µ = 0.1 M KCl, to calculate the protonation constants of the ligand and formation constants of metal complexes formed by the ligand with Fe(III), Al(III), and Cr(III) metal ions. The affinity of the ligand towards Fe(III) is compared with deferiprone (a drug applied for iron intoxication) and transferrin (the main Fe(III) binding protein in plasma). Structural analysis of the ligand and the metal complexes was done using semiempirical PM6 method. Electronic and IR spectra are calculated by semiempirical methods and compared with experimental one. PMID:25294978

  15. 40 CFR Table 2 to Subpart III of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart III

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (40 CFR Part 63, Subpart A) to Subpart III 2 Table 2 to Subpart III of Part 63 Protection of... Pollutants for Flexible Polyurethane Foam Production Pt. 63, Subpt. III, Table 2 Table 2 to Subpart III of Part 63—Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart III Subpart...

  16. 40 CFR Table 2 to Subpart III of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart III

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CFR Part 63, Subpart A) to Subpart III 2 Table 2 to Subpart III of Part 63 Protection of Environment... Flexible Polyurethane Foam Production Pt. 63, Subpt. III, Table 2 Table 2 to Subpart III of Part 63—Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart III Subpart A reference...

  17. Variation in plasmonic (electronic) spectral parameters of Pr (III) and Nd (III) with varied concentration of moderators

    SciTech Connect

    Mishra, Shubha; Limaye, S. N.

    2015-07-31

    It is said that the -4f shells behave as core and are least perturbed by changes around metal ion surrounding. However, there are evidences that-4f shells partially involved in direct moderator interaction. A systematic investigation on the plasmonic (electronic) spectral studies of some Rare Earths[RE(III).Mod] where, RE(III) = Pr(III),Nd(III) and Mod(moderator) = Y(III),La(III),Gd(III) and Lu(III), increased moderator concentration from 0.01 mol dm{sup −3} to 0.025 mol dm{sup −3} keeping the metal ion concentration at 0.01mol dm{sup −3} have been carried out. Variations in oscillator strengths (f), Judd-Ofelt parameters (T{sub λ}),inter-electronic repulsion Racah parameters (δE{sup k}),nephelauxetic ratio (β), radiative parameters (S{sub ED},A{sub T},β{sub R},T{sub R}). The values of oscillator strengths and Judd-Ofelt parameters have been discussed in the light of coordination number of RE(III) metal ions, denticity and basicity of the moderators. The [RE(III).Mod] bonding pattern has been studies in the light of the change in Racah parameters and nephelauxetic ratio.

  18. III-V nanowires and nanowire optoelectronic devices

    NASA Astrophysics Data System (ADS)

    Zhang, Yunyan; Wu, Jiang; Aagesen, Martin; Liu, Huiyun

    2015-11-01

    III-V nanowires (NWs) have been envisioned as nanoscale materials for next-generation technology with good functionality, superior performance, high integration ability and low cost, because of their special growth modes and unique 1D structure. In this review, we summarize the main challenges and important progress of the fabrication and applications of III-V NWs. We start with the III-V NW growth, that significantly influences the NW morphology and crystal quality. Attention is then given to the fabrication of some advanced III-V structures composed of axial and radial junctions. After that, we review the advantages, challenges, and major breakthroughs of using III-V NWs as solar energy harvesters and light emitters. Finally, we attempt to give a perspective look on the future development trends and the remaining challenges in the research field of III-V NWs.

  19. Apolipophorin III: a lipid-triggered molecular switch.

    PubMed

    Weers, Paul M M; Ryan, Robert O

    2003-12-01

    Apolipophorin III (apoLp-III) is a low molecular weight exchangeable apolipoprotein that plays an important role in the enhanced neutral lipid transport during insect flight. The protein exists in lipid-free and lipid-bound states. The lipid-bound state is the active form of the protein and occurs when apoLp-III associates with lipid-enriched lipophorins. ApoLp-III is well characterized in two evolutionally divergent species: Locusta migratoria and Manduca sexta. The two apolipoproteins interact in a similar manner with model phospholipid vesicles, and transform them into discoidal particles. Their low intrinsic stability in the lipid-free state likely facilitates interaction with lipid surfaces. Low solution pH also favors lipid binding interaction through increased exposure of hydrophobic surfaces on apoLp-III. While secondary structure is maintained under acidic conditions, apoLp-III tertiary structure is altered, adopting molten globule-like characteristics. In studies of apoLp-III interaction with natural lipoproteins, we found that apoLp-III is readily displaced from the surface of L. migratoria low-density lipophorin by recombinant apoLp-III proteins from either L. migratoria or M. sexta. Thus, despite important differences between these two apoLp-IIIs (amino acid sequence, presence of carbohydrate), their functional similarity is striking. This similarity is also illustrated by the recently published NMR solution structure of M. sexta apoLp-III wherein its molecular architecture closely parallels that of L. migratoria apoLp-III. PMID:14599497

  20. ORTHODONTIC TREATMENT ALTERNATIVE TO A CLASS III SUBDIVISION MALOCCLUSION

    PubMed Central

    Janson, Guilherme; de Souza, José Eduardo Prado; Barros, Sérgio Estelita Cavalcante; Andrade, Pedro; Nakamura, Alexandre Yudi

    2009-01-01

    Class III malocclusions are considered one of the most complex and difficult orthodontic problems to diagnose and treat. Skeletal and/or dental asymmetries in patients presenting with Class III malocclusions can worsen the prognosis. Recognizing the dentoalveolar and skeletal characteristics of subdivision malocclusions and their treatment possibilities is essential for a favorable nonsurgical correction. Therefore, this article presents a nonsurgical asymmetric extraction approach to Class III subdivision malocclusion treatment which can significantly improve the occlusal and facial discrepancies. PMID:19668997

  1. AUTOCLASS III - AUTOMATIC CLASS DISCOVERY FROM DATA

    NASA Technical Reports Server (NTRS)

    Cheeseman, P. C.

    1994-01-01

    The program AUTOCLASS III, Automatic Class Discovery from Data, uses Bayesian probability theory to provide a simple and extensible approach to problems such as classification and general mixture separation. Its theoretical basis is free from ad hoc quantities, and in particular free of any measures which alter the data to suit the needs of the program. As a result, the elementary classification model used lends itself easily to extensions. The standard approach to classification in much of artificial intelligence and statistical pattern recognition research involves partitioning of the data into separate subsets, known as classes. AUTOCLASS III uses the Bayesian approach in which classes are described by probability distributions over the attributes of the objects, specified by a model function and its parameters. The calculation of the probability of each object's membership in each class provides a more intuitive classification than absolute partitioning techniques. AUTOCLASS III is applicable to most data sets consisting of independent instances, each described by a fixed length vector of attribute values. An attribute value may be a number, one of a set of attribute specific symbols, or omitted. The user specifies a class probability distribution function by associating attribute sets with supplied likelihood function terms. AUTOCLASS then searches in the space of class numbers and parameters for the maximally probable combination. It returns the set of class probability function parameters, and the class membership probabilities for each data instance. AUTOCLASS III is written in Common Lisp, and is designed to be platform independent. This program has been successfully run on Symbolics and Explorer Lisp machines. It has been successfully used with the following implementations of Common LISP on the Sun: Franz Allegro CL, Lucid Common Lisp, and Austin Kyoto Common Lisp and similar UNIX platforms; under the Lucid Common Lisp implementations on VAX/VMS v5

  2. J/psi spectroscopy from Mark III

    SciTech Connect

    Mallik, U.

    1987-02-01

    The Mark III detector at the SPEAR e/sup +/e/sup -/ storage ring at SLAC has accumulated a data sample of 5.8 x 10/sup 6/ J/psi produced. The status of the xi(2230) observed in the radiative J/psi decay is described. The status of the glueball candidates eta(1440) (iota(1440)) and f/sub 2/(1720) (theta(1720)) are probed with a systematic comparison between the radiative and the hadronic decays of J/psi. Finally, an understanding of quark correlations is attempted from a systematic study of the J/psi decaying into Vector-Pseudoscalar, Vector-Tensor and Vector-Scalar nonets.

  3. The NATO III 5 MHz Distribution System

    NASA Technical Reports Server (NTRS)

    Vulcan, A.; Bloch, M.

    1981-01-01

    A high performance 5 MHz distribution system is described which has extremely low phase noise and jitter characteristics and provides multiple buffered outputs. The system is completely redundant with automatic switchover and is self-testing. Since the 5 MHz reference signals distributed by the NATO III distribution system are used for up-conversion and multiplicative functions, a high degree of phase stability and isolation between outputs is necessary. Unique circuit design and packaging concepts insure that the isolation between outputs is sufficient to quarantee a phase perturbation of less than 0.0016 deg when other outputs are open circuited, short circuited or terminated in 50 ohms. Circuit design techniques include high isolation cascode amplifiers. Negative feedback stabilizes system gain and minimizes circuit phase noise contributions. Balanced lines, in lieu of single ended coaxial transmission media, minimize pickup.

  4. POPULATION III GAMMA-RAY BURSTS

    SciTech Connect

    Meszaros, P.; Rees, M. J.

    2010-06-01

    We discuss a model of Poynting-dominated gamma-ray bursts from the collapse of very massive first generation (Pop. III) stars. From redshifts of order 20, the resulting relativistic jets would radiate in the hard X-ray range around 50 keV and above, followed after roughly a day by an external shock component peaking around a few keV. On the same timescales an inverse Compton component around 75 GeV may be expected, as well as a possible infrared flash. The fluences of these components would be above the threshold for detectors such as Swift and Fermi, providing potentially valuable information on the formation and properties of what may be the first luminous objects and their black holes in the high redshift universe.

  5. Propellant management operations on GSTAR-III

    NASA Astrophysics Data System (ADS)

    Oberg, D. J.; Misra, P.

    1993-06-01

    The GSTAR III satellite has depleted most of its hydrazine fuel supply, and will continue to operate with nearly empty fuel tanks for several years by allowing the inclination to grow. Temperature differentials on pairs of tanks within a half system cause the fuel to be pumped back and forth between the tanks on a daily basis. The depletion of any one tank expels helium into the lines, creating maneuver difficulties and an uncertainty in estimation of available fuel. A model of fuel flow, based on the Ideal Gas Law, has been developed to estimate the level of fuel in each tank at all times, and a thermal model of the propulsion system has been developed which uses measured temperatures to estimate convection from flowing hydrazine and verify the fuel flow model. Tank heaters have been used to control fuel distribution with apparent success.

  6. Benchmark On Sensitivity Calculation (Phase III)

    SciTech Connect

    Ivanova, Tatiana; Laville, Cedric; Dyrda, James; Mennerdahl, Dennis; Golovko, Yury; Raskach, Kirill; Tsiboulia, Anatoly; Lee, Gil Soo; Woo, Sweng-Woong; Bidaud, Adrien; Patel, Amrit; Bledsoe, Keith C; Rearden, Bradley T; Gulliford, J.

    2012-01-01

    The sensitivities of the keff eigenvalue to neutron cross sections have become commonly used in similarity studies and as part of the validation algorithm for criticality safety assessments. To test calculations of the sensitivity coefficients, a benchmark study (Phase III) has been established by the OECD-NEA/WPNCS/EG UACSA (Expert Group on Uncertainty Analysis for Criticality Safety Assessment). This paper presents some sensitivity results generated by the benchmark participants using various computational tools based upon different computational methods: SCALE/TSUNAMI-3D and -1D, MONK, APOLLO2-MORET 5, DRAGON-SUSD3D and MMKKENO. The study demonstrates the performance of the tools. It also illustrates how model simplifications impact the sensitivity results and demonstrates the importance of 'implicit' (self-shielding) sensitivities. This work has been a useful step towards verification of the existing and developed sensitivity analysis methods.

  7. Advanced Emissions Control Development Program: Phase III

    SciTech Connect

    G.T. Amrhein; R.T. Bailey; W. Downs; M.J. Holmes; G.A. Kudlac; D.A. Madden

    1999-07-01

    The primary objective of the Advanced Emissions Control Development Program (AECDP) is to develop practical, cost-effective strategies for reducing the emissions of air toxics from coal-fired boilers. The project goal is to effectively control air toxic emissions through the use of conventional flue gas clean-up equipment such as electrostatic precipitators (ESPs), fabric filters (baghouses - BH), and wet flue gas desulfurization systems (WFGD). Development work concentrated on the capture of trace metals, fine particulate, hydrogen chloride and hydrogen fluoride, with an emphasis on the control of mercury. The AECDP project is jointly funded by the US Department of Energy's Federal Energy Technology Center (DOE), the Ohio Coal Development Office within the Ohio Department of Development (OCDO), and Babcock and Wilcox, a McDermott company (B and W). This report discusses results of all three phases of the AECDP project with an emphasis on Phase III activities. Following the construction and evaluation of a representative air toxics test facility in Phase I, Phase II focused on characterization of the emissions of mercury and other air toxics and the control of these emissions for typical operating conditions of conventional flue gas clean-up equipment. Some general comments that can be made about the control of air toxics while burning a high-sulfur bituminous coal are as follows: (1) particulate control devices such as ESP's and baghouses do a good job of removing non-volatile trace metals, (2) particulate control devices (ESPs and baghouses) effectively remove the particulate-phase mercury, but the particulate-phase mercury was only a small fraction of the total for the coals tested, (3) wet scrubbing can effectively remove hydrogen chloride and hydrogen fluoride, and (4) wet scrubbers show good potential for the removal of mercury when operated under certain conditions, however, for certain applications, system enhancements can be required to achieve high

  8. Neptunium(III) copper(I) diselenide

    PubMed Central

    Wells, Daniel M.; Skanthakumar, S.; Soderholm, L.; Ibers, James A.

    2009-01-01

    The title compound, NpCuSe2, is the first ternary neptunium transition-metal chalcogenide. It was synthesized from the elements at 873 K in an evacuated fused-silica tube. Single crystals were grown by vapor transport with I2. NpCuSe2 crystallizes in the LaCuS2 structure type and can be viewed as a stacking of layers of CuSe4 tetra­hedra and of double layers of NpSe7 monocapped trigonal prisms along [100]. Because there are no Se—Se bonds in the structure, the formal oxidation states of Np/Cu/Se may be assigned as +III/+I/−II, respectively. PMID:21582032

  9. Aqua-bromidobis(dimethyl-glyoximato)cobalt(III).

    PubMed

    Meera, Parthasarathy; Amutha Selvi, Madhavan; Jothi, Pachaimuthu; Dayalan, Arunachalam

    2011-04-01

    In the title complex, [CoBr(C(4)H(7)N(2)O(2))(2)(H(2)O)], a crystallo-graphic mirror plane bis-ects the mol-ecule, perpendicular to the glyoximate ligands. The geometry around the cobalt(III) atom is approximately octa-hedral with the four glyoximate N atoms forming the square base. A bromide ion and the O atom of a water mol-ecule occupy the remaining coordination sites. The N-Co-N bite angles are 82.18 (4) and 80.03 (16)°. The glyoximate moieties form strong intra-molecular O-H⋯O hydrogen bonds. The coordinated water mol-ecule forms an inter-molecular O-H⋯O hydrogen bond with a glyoximate O atom, thereby generating supra-molecular chains parallel to [010]. PMID:21753964

  10. Titan III - Commercial access to space

    NASA Astrophysics Data System (ADS)

    Gizinski, Stephen J., III; Herrington, Douglas B.

    1988-06-01

    The commercial Titan III launch vehicle is discussed, reviewing the history of the Titan program, the technical aspects of the launcher, and the market outlook. The solid rocket motors of the boost vehicle, core, attitude control system, and payload carrier are described. The vehicle can carry one or two payloads taking up a space of up to 3.65 m in diameter and 10.7 m in length. The avionics, communications, and electrical power systems of the vehicle are examined and the range of perigree stages with which the vehicle is compatible is given. An overview of the mission and the launch facilities is presented and future markets for commercial satellites are considered.

  11. DECIMETRIC TYPE III BURSTS: GENERATION AND PROPAGATION

    SciTech Connect

    Li, B.; Cairns, Iver H.; Robinson, P. A.; Yan, Y. H.

    2011-09-01

    Simulations are presented for decimetric type III radio bursts at 2f{sub p} , where f{sub p} is the local electron plasma frequency. The simulations show that 2f{sub p} radiation can be observed at Earth in two scenarios for the radiation's generation and propagation. In Scenario A, radiation is produced and propagates in warm plasmas in the lower corona that are caused by previous magnetic reconnection outflows and/or chromospheric evaporation. In Scenario B radiation is generated in normal plasmas, then due to its natural directivity pattern and refraction, radiation partly propagates into nearby regions, which are hot because of previous reconnection/evaporation. The profiles of plasma density n{sub e} (r) and electron temperature T{sub e} (r) in the lower corona (r - R{sub sun} {approx}< 100 Mm) are found to be crucial to whether radiation can be produced and escape at observable levels against the effects of free-free absorption, where r is the heliocentric distance. Significantly, the observed wide ranges of radiation properties (e.g., drift rates) require n{sub e} (r) with a large range of scale heights h{sub s} , consistent nonetheless for Scenario B with short observed EUV loops. This is relevant to problems with large h{sub s} inferred from tall EUV loops. The simulations suggest: (1) n{sub e} (r) with small h{sub s} , such as n{sub e} (r){proportional_to}(r - R{sub sun}){sup -2.38} for flaring regions, are unexpectedly common deep in the corona. This result is consistent with recent work on n{sub e} (r) for r {approx} (1.05-2)R{sub sun} extracted from observed metric type IIIs. (2) The dominance of reverse-slope bursts over normal bursts sometimes observed may originate from asymmetric reconnection/acceleration, which favors downgoing beams.

  12. A possible role for chromium(III) in genotoxicity.

    PubMed Central

    Snow, E T

    1991-01-01

    Chromium is found in the environment in two major forms: reduced CrIII and CrVI, or chromate. Chromate, the most biologically active species, is readily taken up by living cells and reduced intracellularly, via reactive intermediates, to stable CrIII species. CrIII, the most abundant form of chromium in the environment, does not readily cross cell membranes and is relatively inactive in vivo. However, intracellular CrIII can react slowly with both nucleic acids and proteins and can be genotoxic. We have investigated the genotoxicity of CrIII in vitro using a DNA replication assay and in vivo by CaCl2-mediated transfection of chromium-treated DNA into Escherichia coli. When DNA replication was measured on a CrIII-treated template using purified DNA polymerases (either bacterial or mammalian), both the rate of DNA replication and the amount of incorporation per polymerase binding event (processivity) were greatly increased relative to controls. When transfected into E. coli, CrIII-treated M13mp2 bacteriophage DNA showed a dose-dependent increase in mutation frequency. These results suggest that CrIII alters the interaction between the DNA template and the polymerase such that the binding strength of the DNA polymerase is increased and the fidelity of DNA replication is decreased. These interactions may contribute to the mutagenicity of chromium ions in vivo and suggest that CrIII can contribute to chromium-mediated carcinogenesis. Images FIGURE 3. PMID:1935855

  13. Expression of Secretogranin III in Chicken Endocrine Cells

    PubMed Central

    Morikawa, Satomi; Shinmura, Naoki; Moki, Hiroaki; Yasui, Tadashi; Tsukise, Azuma; Torii, Seiji; Watanabe, Tsuyoshi; Maeda, Yoshinori; Hosaka, Masahiro

    2015-01-01

    The expression of secretogranin III (SgIII) in chicken endocrine cells has not been investigated. There is limited data available for the immunohistochemical localization of SgIII in the brain, pituitary, and pancreatic islets of humans and rodents. In the present study, we used immunoblotting to reveal the similarities between the expression patterns of SgIII in the common endocrine glands of chickens and rats. The protein–protein interactions between SgIII and chromogranin A (CgA) mediate the sorting of CgA/prohormone core aggregates to the secretory granule membrane. We examined these interactions using co-immunoprecipitation in chicken endocrine tissues. Using immunohistochemistry, we also examined the expression of SgIII in a wide range of chicken endocrine glands and gastrointestinal endocrine cells (GECs). SgIII was expressed in the pituitary, pineal, adrenal (medullary parts), parathyroid, and ultimobranchial glands, but not in the thyroid gland. It was also expressed in GECs of the stomach (proventriculus and gizzard), small and large intestines, and pancreatic islet cells. These SgIII-expressing cells co-expressed serotonin, somatostatin, gastric inhibitory polypeptide, glucagon-like peptide-1, glucagon, or insulin. These results suggest that SgIII is expressed in the endocrine cells that secrete peptide hormones, which mature via the intragranular enzymatic processing of prohormones and physiologically active amines in chickens. PMID:25673289

  14. Antisites in III-V semiconductors: Density functional theory calculations

    SciTech Connect

    Chroneos, A.; Tahini, H. A.; Schwingenschlögl, U.; Grimes, R. W.

    2014-07-14

    Density functional based simulation, corrected for finite size effects, is used to investigate systematically the formation of antisite defects in III-V semiconductors (III = Al, Ga, and In and V = P, As, and Sb). Different charge states are modelled as a function of the Fermi level and under different growth conditions. The formation energies of group III antisites (III{sub V}{sup q}) decrease with increasing covalent radius of the group V atom though not group III radius, whereas group V antisites (V{sub III}{sup q}) show a consistent decrease in formation energies with increase in group III and group V covalent radii. In general, III{sub V}{sup q} defects dominate under III-rich conditions and V{sub III}{sup q} under V-rich conditions. Comparison with equivalent vacancy formation energy simulations shows that while antisite concentrations are always dominant under stoichiometric conditions, modest variation in growth or doping conditions can lead to a significantly higher concentration of vacancies.

  15. Synthesis, characterization, molecular docking and DNA binding studies of Al(III), Ga(III) and In(III) water-soluble complexes

    NASA Astrophysics Data System (ADS)

    Shorkaei, Mohammad Ranjkesh; Asadi, Zahra; Asadi, Mozaffar

    2016-04-01

    In this work three new water-soluble aluminum(III), gallium(III) and indium(III) Schiff base complexes; Na2[M(L)NO3]; where L denotes; N,N'-bis(5-sulfosalicyliden)-1,2-phenylendiamin (salsophen) were synthesized and characterized by UV-vis, 1HNMR, FT-IR spectroscopy, thermal gravimetry (TG) and elemental analysis. To study the biological preference with the molecular target DNA, interaction of these complexes with DNA have been explored by employing various biophysical methods including absorption spectra, fluorescence spectra, cyclic voltammetry and viscosity measurement. The Kb values at 298 K were found to be 1.17 × 104 for Al(III), 1.35 × 104 for Ga(III) and 1.64 × 104 M-1 for In(III) complexes, respectively. These results suggesting the greater binding propensity of In(III) complexes. Additionally molecular docking was carried out to ascertain the mode of action towards the molecular target DNA.

  16. Selective Extraction of Americium(III) over Europium(III) with the Pyridylpyrazole Based Tetradentate Ligands: Experimental and Theoretical Study.

    PubMed

    Wang, Jieru; Su, Dongping; Wang, Dongqi; Ding, Songdong; Huang, Chao; Huang, Huang; Hu, Xiaoyang; Wang, Zhipeng; Li, Shimeng

    2015-11-16

    1,3-Bis[3-(2-pyridyl)pyrazol-1-yl]propane (Bippp) and 1,2-bis[3-(2-pyridyl)pyrazyl-1-methyl]benzene (Dbnpp), the pyridylpyrazole based tetradentate ligands, were synthesized and characterized by MS, NMR, and FT-IR. The solvent extraction and complexation behaviors of Am(III) and Eu(III) with the ligands were investigated experimentally and theoretically. In the presence of 2-bromohexanoic acid, the two ligands can effectively extract Am(III) over Eu(III) and other rare earth(III) metals (RE(III)) in HNO3 solution with the separation factors (SFAm/RE) ranging from 15 to 60. Slope analyses showed that both Am(III) and Eu(III) were extracted as monosolvated species, which agrees well with the results observed from X-ray crystallography and MS analyses. The stability constants (log K) obtained from UV-vis titration for Eu(III) complexes with Bippp and Dbnpp are 4.75 ± 0.03 and 4.45 ± 0.04, respectively. Both UV-vis titration and solvent extraction studies indicated that Bippp had stronger affinity for Eu(III) than Dbnpp, which is confirmed by density functional theory (DFT) calculations. DFT calculations revealed that the AmL(NO3)3 (L = Bippp and Dbnpp) complexes are thermodynamically more stable in water than their Eu(III) analogues, which is caused by greater covalency of the Am-N than Eu-N bonds. Theoretical studies gave an insight into the nature of the M(III)-ligand bonding interactions. PMID:26517736

  17. Oxalate complexation with aluminum(III) and iron(III) at moderately elevated temperatures

    SciTech Connect

    Tait, C.D.; Janecky, D.R.; Clark, D.L.; Bennett, P.C.

    1992-05-01

    To add to our understanding of the weathering of rocks in organic rich environments such as sedimentary brines and oil field waters, we have examined the temperature dependent complexation of aluminum with oxalate. Raman vibrational studies show that even the association constant for the highly charged Al(ox){sub 3}{sup 3{minus}} unexpectedly increases with moderate temperature increases to 80{degrees}C. To evaluate the potential importance of these Al-oxalate species in complex natural systems, temperature dependent competition experiments Fe(III) and Al(III) for oxalate have been initiated. Similar to aluminum, ferric oxalates show increases in association constants at higher temperatures. In competition experiments, the first association constant for Fe(ox){sup +} increases faster than that for Al(ox){sup +} to 90{degrees}C.

  18. Preparation and spectroscopic studies of antimony(III) and bismuth(III) halodithiocarbamate derivatives

    NASA Astrophysics Data System (ADS)

    Giusti, Aleardo; Preti, Carlo; Tosi, Giuseppe; Zannini, Paolo

    1983-04-01

    The complexes of antimony(III) and bismuth(III) with piperidine (Pipdtc), morpholine (Morphdtc) and thiomorpholinedithiocarbamate (Timdtc) of general formula Sb 2-(Rdtc) 3X 3 and M(Rdtc)X 2 (M is antimony or bismuth, X a halogen and Rdtc the dithiocarbamates) have been prepared and characterized by spectroscopic methods. The IR spectra suggest that the dithiocarbamate group coordinates as a bidentate ligand; the metal-sulphur and metal-halide stretching modes have also been assigned. The spectral data are discussed and compared with those of the corresponding trisdithiocarbamate and monohalobisdithiocarbamate derivatives. The molecular weight determinations indicate that all these dithiocarbamate complexes are dimeric. Tentative stereochemistries are proposed and discussed on the basis of the results obtained.

  19. Oxalate complexation with aluminum(III) and iron(III) at moderately elevated temperatures

    SciTech Connect

    Tait, C.D.; Janecky, D.R.; Clark, D.L. ); Bennett, P.C. . Dept. of Geological Sciences)

    1992-01-01

    To add to our understanding of the weathering of rocks in organic rich environments such as sedimentary brines and oil field waters, we have examined the temperature dependent complexation of aluminum with oxalate. Raman vibrational studies show that even the association constant for the highly charged Al(ox){sub 3}{sup 3{minus}} unexpectedly increases with moderate temperature increases to 80{degrees}C. To evaluate the potential importance of these Al-oxalate species in complex natural systems, temperature dependent competition experiments Fe(III) and Al(III) for oxalate have been initiated. Similar to aluminum, ferric oxalates show increases in association constants at higher temperatures. In competition experiments, the first association constant for Fe(ox){sup +} increases faster than that for Al(ox){sup +} to 90{degrees}C.

  20. A Qualitative Study of Recovery from Type III-B and III-C Tibial Fractures

    PubMed Central

    Shauver, Melissa S.; Aravind, Maya S.; Chung, Kevin C.

    2011-01-01

    The literature has shown that long-term outcomes for both below-knee amputation and reconstruction following type III-B and III-C tibial fracture are poor. Yet, patients often report satisfaction with their treatment and/or outcomes. The aim of this study is to explore the relationship between patient outcomes and satisfaction after open tibial fractures via qualitative methodology. Twenty patients who were treated for open tibial fractures at one institution were selected using purposeful sampling and interviewed in-person in a semi-structured manner. Data were analyzed using grounded theory methodology. Despite reporting marked physical and psychosocial deficits, participants relayed high satisfaction. We hypothesize that the use adaptive coping techniques successfully reduces stress, which leads to an increase in coping self-efficacy that results in the further use of adaptive coping strategies, culminating in personal growth. This stress reduction and personal growth leads to satisfaction despite poor functional and emotional outcomes. PMID:20948418

  1. Exceptional Oxygen Sensing Properties of New Blue Light-Excitable Highly Luminescent Europium(III) and Gadolinium(III) Complexes

    PubMed Central

    Borisov, Sergey M.; Fischer, Roland; Saf, Robert; Klimant, Ingo

    2016-01-01

    New europium(III) and gadolinium(III) complexes bearing 8-hydroxyphenalenone antenna combine efficient absorption in the blue part of the spectrum and strong emission in polymers at room temperature. The Eu(III) complexes show characteristic red luminescence whereas the Gd(III) dyes are strongly phosphorescent. The luminescence quantum yields are about 20% for the Eu(III) complexes and 50% for the Gd(III) dyes. In contrast to most state-of-the-art Eu(III) complexes the new dyes are quenched very efficiently by molecular oxygen. The luminescence decay times of the Gd(III) complexes exceed 1 ms which ensures exceptional sensitivity even in polymers of moderate oxygen permeability. These sensors are particularly suitable for trace oxygen sensing and may be good substitutes for Pd(II) porphyrins. The photophysical and sensing properties can be tuned by varying the nature of the fourth ligand. The narrow-band emission of the Eu(III) allows efficient elimination of the background light and autofluorescence and is also very attractive for use e.g. in multi-analyte sensors. The highly photostable indicators incorporated in nanoparticles are promising for imaging applications. Due to the straightforward preparation and low cost of starting materials the new dyes represent a promising alternative to the state-of-the-art oxygen indicators particularly for such applications as e.g. food packaging. PMID:27158252

  2. Synthesis and Structure of Hexatungstochromate(III), [H3Cr(III)W6O24]6-.

    PubMed

    Liu, Wenjing; Lin, Zhengguo; Bassil, Bassem S; Al-Oweini, Rami; Kortz, Ulrich

    2015-01-01

    The hexatungstochromate(III) [H(3)Cr(III)W(6)O(24)](6-) (1) was synthesized in aqueous, basic medium by simple reaction of chromium(III) nitrate nonahydrate and sodium tungstate dihydrate in a 1:6 ratio. Polyanion 1 represents the first Anderson-Evans type heteropolytungstate with a trivalent hetero element. The sodium salt of 1 with the formula Na(6)[H(3)Cr(III)W(6)O(24)]·22H(2)O (1a) was fully characterized in the solid state by single crystal XRD, FT-IR spectroscopy, and thermogravimetric analysis. PMID:26507761

  3. Soluble Manganese(III) in the Marine Environment

    NASA Astrophysics Data System (ADS)

    Luther, G. W., III; Oldham, V.; Madison, A.; Tebo, B.; Jones, M.; Jensen, L.; Owings, S.; Mucci, A.; Sundby, B.

    2014-12-01

    Recent field studies have confirmed the presence of soluble manganese(III), which along with Mn(II) passes through a 0.2 μm filter, in suboxic marine waters. Here we applied a spectrophotometric method using a soluble porphyrin as a competitive ligand to calculate the concentrations and kinetics of Mn(II) and Mn(III) recovery. Data will be presented from the suboxic porewaters of the Saint Lawrence estuary, the suboxic and anoxic waters of the Chesapeake Bay and the oxygenated surface waters of a coastal waterway bordered by wetlands and salt marshes in Delaware. Soluble Mn(III) accounts for up to 100% of the dissolved Mn pool with concentrations ranging from the detection limit of 50 nM to 80 μM at the oxic/anoxic interface of the non-sulfidic porewaters from the hemipelagic sediments of the St. Lawrence Estuary. Data indicate weak-ligand complexation of Mn(III) formed from Mn(II) oxidation as well as reduction of MnO2. Complexation of Mn(III) in the anoxic waters of Chesapeake Bay appears stronger as the porphyrin could not outcompete the natural ligands binding Mn(III). Mn(III) complexes were reduced in the presence of hydroxylamine or hydrogen sulfide and detected as Mn(II). Soluble Mn(III) comprised up to 52 % of total dissolved Mn. Profiles over the course of a five day cruise showed that high Mn(III) concentrations (7.3 μM) were observed at low H2S (4.9 μM) whereas low Mn(III) (1.1 μM) was detected at high H2S (40 μM). The presence of Mn(III) in sulfidic waters indicated that it is kinetically stabilized in situ by strong ligands so reduction to Mn(II) was incomplete. One electron reductive dissolution of solid MnO2 particles formed at the oxic-anoxic interface appear to be the source of Mn(III). Lastly, soluble Mn(III) was detected in the oxygenated surface waters of a coastal waterway (salinity ranging from freshwater to 31) bordered by wetlands and salt marshes in Delaware. Soluble Mn(III) made up 0-49 % of the total dissolved Mn (maximum of 1.92

  4. Biochemical and Structural Properties of Mouse Kynurenine Aminotransferase III

    SciTech Connect

    Han, Q.; Robinson, H; Cai, T; Tagle, D; Li, J

    2009-01-01

    Kynurenine aminotransferase III (KAT III) has been considered to be involved in the production of mammalian brain kynurenic acid (KYNA), which plays an important role in protecting neurons from overstimulation by excitatory neurotransmitters. The enzyme was identified based on its high sequence identity with mammalian KAT I, but its activity toward kynurenine and its structural characteristics have not been established. In this study, the biochemical and structural properties of mouse KAT III (mKAT III) were determined. Specifically, mKAT III cDNA was amplified from a mouse brain cDNA library, and its recombinant protein was expressed in an insect cell protein expression system. We established that mKAT III is able to efficiently catalyze the transamination of kynurenine to KYNA and has optimum activity at relatively basic conditions of around pH 9.0 and at relatively high temperatures of 50 to 60C. In addition, mKAT III is active toward a number of other amino acids. Its activity toward kynurenine is significantly decreased in the presence of methionine, histidine, glutamine, leucine, cysteine, and 3-hydroxykynurenine. Through macromolecular crystallography, we determined the mKAT III crystal structure and its structures in complex with kynurenine and glutamine. Structural analysis revealed the overall architecture of mKAT III and its cofactor binding site and active center residues. This is the first report concerning the biochemical characteristics and crystal structures of KAT III enzymes and provides a basis toward understanding the overall physiological role of mammalian KAT III in vivo and insight into regulating the levels of endogenous KYNA through modulation of the enzyme in the mouse brain.

  5. Synthesis and structural characterization of new dithiocarbamate complexes from Sb(III) and Bi(III)

    SciTech Connect

    Jamaluddin, Nur Amirah; Baba, Ibrahim

    2013-11-27

    Twenty new antimony and bismuth dithiocarbamate complexes which employed ten different type of amines have been successfully synthesized. The synthesized complexes with metal to dithiocarbamate ratio at 1:3. Elemental analysis of the complexes gave the general formula of MCl[S{sub 2}CNR’R”]{sub 2} where M = Sb(III), Bi(III); R’ = methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, benzyl; R” = ethanol, methyl, ethyl, propyl, isopropyl, cyclohexyl, benzyl. The complexes were analysed by IR and NMR spectroscopy. The crystal structure of five-coordinated antimony (III) complex have been determined by X-ray single crystal diffraction. Single crystal X-ray diffraction studies on SbCl[S{sub 2}CN(C{sub 4}H{sub 9})(C{sub 2}H{sub 5})]{sub 2} adopted a triclinic system with a space group P1 with a = 10.0141(8) Å, b = 10.1394(7) Å, c = 11.8665(9) Å, α = 67.960°, β =87.616°, γ = 80.172°.

  6. Synthesis and structural characterization of new dithiocarbamate complexes from Sb(III) and Bi(III)

    NASA Astrophysics Data System (ADS)

    Jamaluddin, Nur Amirah; Baba, Ibrahim

    2013-11-01

    Twenty new antimony and bismuth dithiocarbamate complexes which employed ten different type of amines have been successfully synthesized. The synthesized complexes with metal to dithiocarbamate ratio at 1:3. Elemental analysis of the complexes gave the general formula of MCl[S2CNR'R"]2 where M = Sb(III), Bi(III); R' = methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, benzyl; R" = ethanol, methyl, ethyl, propyl, isopropyl, cyclohexyl, benzyl. The complexes were analysed by IR and NMR spectroscopy. The crystal structure of five-coordinated antimony (III) complex have been determined by X-ray single crystal diffraction. Single crystal X-ray diffraction studies on SbCl[S2CN(C4H9)(C2H5)]2 adopted a triclinic system with a space group P1 with a = 10.0141(8) Å, b = 10.1394(7) Å, c = 11.8665(9) Å, α = 67.960°, β =87.616°, γ = 80.172°.

  7. Gd(III)-Gd(III) distance measurements with chirp pump pulses

    NASA Astrophysics Data System (ADS)

    Doll, Andrin; Qi, Mian; Wili, Nino; Pribitzer, Stephan; Godt, Adelheid; Jeschke, Gunnar

    2015-10-01

    The broad EPR spectrum of Gd(III) spin labels restricts the dipolar modulation depth in distance measurements between Gd(III) pairs to a few percent. To overcome this limitation, frequency-swept chirp pulses are utilized as pump pulses in the DEER experiment. Using a model system with 3.4 nm Gd-Gd distance, application of one single chirp pump pulse at Q-band frequencies leads to modulation depths beyond 10%. However, the larger modulation depth is counteracted by a reduction of the absolute echo intensity due to the pump pulse. As supported by spin dynamics simulations, this effect is primarily driven by signal loss to double-quantum coherence and specific to the Gd(III) high spin state of S = 7/2. In order to balance modulation depth and echo intensity for optimum sensitivity, a simple experimental procedure is proposed. An additional improvement by 25% in DEER sensitivity is achieved with two consecutive chirp pump pulses. These pulses pump the Gd(III) spectrum symmetrically around the observation position, therefore mutually compensating for dynamical Bloch-Siegert phase shifts at the observer spins. The improved sensitivity of the DEER data with modulation depths on the order of 20% is due to mitigation of the echo reduction effects by the consecutive pump pulses. In particular, the second pump pulse does not lead to additional signal loss if perfect inversion is assumed. Moreover, the compensation of the dynamical Bloch-Siegert phase prevents signal loss due to spatial dependence of the dynamical phase, which is caused by inhomogeneities in the driving field. The new methodology is combined with pre-polarization techniques to measure long distances up to 8.6 nm, where signal intensity and modulation depth become attenuated by long dipolar evolution windows. In addition, the influence of the zero-field splitting parameters on the echo intensity is studied with simulations. Herein, larger sensitivity is anticipated for Gd(III) complexes with zero

  8. CONVERSION EXTRACTION DESULFURIZATION (CED) PHASE III

    SciTech Connect

    James Boltz

    2005-03-01

    This project was undertaken to refine the Conversion Extraction Desulfurization (CED) technology to efficiently and economically remove sulfur from diesel fuel to levels below 15-ppm. CED is considered a generic term covering all desulfurization processes that involve oxidation and extraction. The CED process first extracts a fraction of the sulfur from the diesel, then selectively oxidizes the remaining sulfur compounds, and finally extracts these oxidized materials. The Department of Energy (DOE) awarded Petro Star Inc. a contract to fund Phase III of the CED process development. Phase III consisted of testing a continuous-flow process, optimization of the process steps, design of a pilot plant, and completion of a market study for licensing the process. Petro Star and the Degussa Corporation in coordination with Koch Modular Process Systems (KMPS) tested six key process steps in a 7.6-centimeter (cm) (3.0-inch) inside diameter (ID) column at gas oil feed rates of 7.8 to 93.3 liters per hour (l/h) (2.1 to 24.6 gallons per hour). The team verified the technical feasibility with respect to hydraulics for each unit operation tested and successfully demonstrated pre-extraction and solvent recovery distillation. Test operations conducted at KMPS demonstrated that the oxidation reaction converted a maximum of 97% of the thiophenes. The CED Process Development Team demonstrated that CED technology is capable of reducing the sulfur content of light atmospheric gas oil from 5,000-ppm to less than 15-ppm within the laboratory scale. In continuous flow trials, the CED process consistently produced fuel with approximately 20-ppm of sulfur. The process economics study calculated an estimated process cost of $5.70 per product barrel. The Kline Company performed a marketing study to evaluate the possibility of licensing the CED technology. Kline concluded that only 13 refineries harbored opportunity for the CED process. The Kline study and the research team's discussions with

  9. 40 CFR Appendixes II-Iii to Part 264 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false II Appendixes II-III to Part 264 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Appendixes II-III...

  10. Avatar Gender and Ethical Choices in "Fable III"

    ERIC Educational Resources Information Center

    Schrier, Karen

    2012-01-01

    This study investigates how players make ethical decisions in "Fable III," a video game, with consideration to avatar gender. Thirty males, 18 to 34 years old, were recruited; 20 were assigned to play "Fable III," with half assigned to play as a male avatar (Condition 1), and half assigned as a female avatar (Condition 2). Any ethical thinking…

  11. VAC protocol for treatment of dogs with stage III hemangiosarcoma.

    PubMed

    Alvarez, Francisco J; Hosoya, Kenji; Lara-Garcia, Ana; Kisseberth, William; Couto, Guillermo

    2013-01-01

    Hemangiosarcomas (HSAs) are aggressive tumors with a high rate of metastasis. Clinical stage has been considered a negative prognostic factor for survival. The study authors hypothesized that the median survival time (MST) of dogs with metastatic (stage III) HSA treated with a vincristine, doxorubicin, and cyclophosphamide (VAC) chemotherapy protocol would not be different than those with stage I/II HSA. Sixty-seven dogs with HSA in different anatomic locations were evaluated retrospectively. All dogs received the VAC protocol as an adjuvant to surgery (n = 50), neoadjuvant (n = 3), or as the sole treatment modality (n = 14). There was no significant difference (P = 0.97) between the MST of dogs with stage III and stage I/II HSA. For dogs presenting with splenic HSA alone, there was no significant difference between the MST of dogs with stage III and stage I/II disease (P = 0.12). The overall response rate (complete response [CR] and partial response [PR]) was 86%). No unacceptable toxicities were observed. Dogs with stage III HSA treated with the VAC protocol have a similar prognosis to dogs with stage I/II HSA. Dogs with HSA and evidence of metastases at the time of diagnosis should not be denied treatment. PMID:24051260

  12. Age Dedifferentiation Hypothesis: Evidence form the WAIS III.

    ERIC Educational Resources Information Center

    Juan-Espinosa, Manuel; Garcia, Luis F.; Escorial, Sergio; Rebollo, Irene; Colom, Roberto; Abad, Francisco J.

    2002-01-01

    Used the Spanish standardization of the Wechsler Adult Intelligence Scale III (WAIS III) (n=1,369) to test the age dedifferentiation hypothesis. Results show no changes in the percentage of variance accounted for by "g" and four group factors when restriction of range is controlled. Discusses an age indifferentation hypothesis. (SLD)

  13. 40 CFR Table 1 to Subpart III of... - Emission Limitations

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 8 2010-07-01 2010-07-01 false Emission Limitations 1 Table 1 to... Before November 30, 1999 Pt. 62, Subpt. III, Table 1 Table 1 to Subpart III of Part 62—Emission Limitations For the air pollutant You must meet this emission limitation a Using this averaging time...

  14. An Exploration of Challenges Facing Division III Athletic Directors

    ERIC Educational Resources Information Center

    Engbers, Jeffrey L.

    2011-01-01

    The purpose of this study was to establish a basic understanding of the challenges associated with directing athletic programs at NCAA Division III Institutions. Specifically, this study identified the frequency, intensity, and time allocated to common challenges facing the position of the NCAA Division III AD. The challenges were examined using…

  15. Genome sequesnce of lineage III Listeria monocytogenes strain HCC23

    Technology Transfer Automated Retrieval System (TEKTRAN)

    More than 98% of reported human listeriosis cases are caused by Listeria monocytogenes serotypes within lineages I and II. Serotypes within lineage III (4a and 4c) are commonly isolated from environmental and food specimens. We report the first complete genome sequence of a lineage III isolate, HCC2...

  16. 32 CFR 2003.3 - Functions (Article III).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Functions (Article III). 2003.3 Section 2003.3 National Defense Other Regulations Relating to National Defense INFORMATION SECURITY OVERSIGHT OFFICE...) BYLAWS, RULES, AND APPEAL PROCEDURES Bylaws § 2003.3 Functions (Article III). In carrying out its...

  17. 32 CFR 2003.3 - Functions (Article III).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Functions (Article III). 2003.3 Section 2003.3 National Defense Other Regulations Relating to National Defense INFORMATION SECURITY OVERSIGHT OFFICE...) BYLAWS, RULES, AND APPEAL PROCEDURES Bylaws § 2003.3 Functions (Article III). In carrying out its...

  18. 7. Photograph of a line drawing. 'PART III, SECTION 1, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDING NO. 10, PRODUCER GAS & EXHAUSTER BLDG., PLANT A.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant A, Parts I, II, III. (Nashville, TN: Office of District Engineer, 1944). - Holston Army Ammunition Plant, Producer Gas Plant, Kingsport, Sullivan County, TN

  19. Extrapyramidal Symptoms and Medication Use in Mucopolysaccharidosis Type III

    ERIC Educational Resources Information Center

    Tchan, Michel C.; Sillence, David

    2009-01-01

    Background: We report the case of a 16-year-old male with Mucopolysaccharidosis III type A (Sanfilippo syndrome) who was commenced on risperidone for behaviour management. He rapidly developed extrapyramidal symptoms that have not resolved. Method: The medication histories of 20 patients with Mucopolysaccharidosis III seen at a Lysosomal Storage…

  20. Molecular structures of unbound and transcribing RNA polymerase III

    PubMed Central

    Hoffmann, Niklas A.; Jakobi, Arjen J.; Moreno-Morcillo, Maria; Glatt, Sebastian; Kosinski, Jan; Hagen, Wim J. H.; Sachse, Carsten; Müller, Christoph W.

    2015-01-01

    Transcription of genes encoding small structured RNAs such as tRNAs, spliceosomal U6 snRNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase. The cryo-EM structures of the S. cerevisiae Pol III elongating complex at 3.9 Å resolution and the apo Pol III enzyme in two different conformations at 4.6 and 4.7 Å resolution, respectively, allow for the first time to build a 17-subunit atomic model of Pol III. The reconstructions reveal the precise orientation of the C82/C34/C31 heterotrimer in close proximity to the stalk. The C53/C37 heterodimer positions residues involved in transcription termination close to the non-template DNA strand. In the apo Pol III structures, the stalk adopts different orientations coupled with closed and open conformations of the clamp. Our results provide novel insights into Pol III-specific transcription and the adaptation of Pol III towards its small transcriptional targets. PMID:26605533

  1. 40 CFR Appendixes I-Ii to Part 268 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false I Appendixes I-II to Part 268 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) LAND DISPOSAL RESTRICTIONS Appendixes I-II to Part 268...

  2. Interpretation of the WISC-III and Its Subtests.

    ERIC Educational Resources Information Center

    Nicholson, Charles L.; Alcorn, Charles L.

    The use of the Wechsler Intelligence Scale for Children, Third Edition (WISC-III) and its interpretation in educational use are discussed. To measure intelligence, Wechsler believed one must measure the various aptitudes that contribute to the total behavior of the individual. The WISC-III has six verbal subtests and seven performance subtests.…

  3. 25 CFR 522.12 - Revocation of class III gaming.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Revocation of class III gaming. 522.12 Section 522.12 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR APPROVAL OF CLASS II AND CLASS III ORDINANCES AND RESOLUTIONS SUBMISSION OF GAMING ORDINANCE OR RESOLUTION § 522.12 Revocation of class...

  4. 40 CFR Table 1 to Subpart III of... - Emission Limitations

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 8 2011-07-01 2011-07-01 false Emission Limitations 1 Table 1 to... Before November 30, 1999 Pt. 62, Subpt. III, Table 1 Table 1 to Subpart III of Part 62—Emission Limitations For the air pollutant You must meet this emission limitation a Using this averaging time...

  5. 40 CFR Table 1 to Subpart III of... - Emission Limitations

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 9 2014-07-01 2014-07-01 false Emission Limitations 1 Table 1 to... Before November 30, 1999 Pt. 62, Subpt. III, Table 1 Table 1 to Subpart III of Part 62—Emission Limitations For the air pollutant You must meet this emission limitation a Using this averaging time...

  6. 77 FR 64397 - Order of Succession for HUD Region III

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-19

    ... URBAN DEVELOPMENT Order of Succession for HUD Region III AGENCY: Office of Field Policy and Management, HUD. ACTION: Notice of Order of Succession. SUMMARY: In this notice, the Assistant Deputy Secretary... Order of Succession for the Philadelphia Regional Office and its Field Offices (Region III). This...

  7. 40 CFR Appendixes I-Ii to Part 268 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false I Appendixes I-II to Part 268 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) LAND DISPOSAL RESTRICTIONS Appendixes I-II to Part 268...

  8. Occurrence and speciation of polymeric chromium(III), monomeric chromium(III) and chromium(VI) in environmental samples.

    PubMed

    Hu, Ligang; Cai, Yong; Jiang, Guibin

    2016-08-01

    Laboratory experiments suggest that polymeric Cr(III) could exist in aqueous solution for a relative long period of time. However, the occurrence of polymeric Cr(III) has not been reported in environmental media due partially to the lack of method for speciating polymeric Cr. We observed an unknown Cr species during the course of study on speciation of Cr in the leachates of chromated-copper-arsenate (CCA)-treated wood. Efforts were made to identify structure of the unknown Cr species. Considering the forms of Cr existed in the CCA-treated woods, we mainly focused our efforts to determine if the unknown species were polymeric Cr(III), complex of Cr/As or complex of Cr with dissolved organic matter (DOM). In order to evaluate whether polymeric Cr(III) largely exist in wood leachates, high performance liquid chromatography coupled with inductively coupled mass spectrometry (HPLC-ICPMS was used) for simultaneous speciation of monomeric Cr(III), polymeric Cr(III), and Cr(VI). In addition to wood leachates where polymeric Cr (III) ranged from 39.1 to 67.4%, occurrence of the unknown Cr species in other environmental matrices, including surface waters, tap and waste waters, was also investigated. It was found that polymeric Cr(III) could exist in environmental samples containing μg/L level of Cr, at a level up to 60% of total Cr, suggesting that polymeric Cr(III) could significantly exist in natural environments. Failure in quantifying polymeric Cr(III) would lead to the underestimation of total Cr and bias in Cr speciation. The environmental implication of the presence of polymeric Cr(III) species in the environment deserves further study. PMID:27156211

  9. Complexation of trivalent cations (Al(III), Cr(III), Fe(III)) with two phosphonic acids in the pH range of fresh waters.

    PubMed

    Lacour, S; Deluchat, V; Bollinger, J C; Bernard Serpaud

    1998-08-01

    The complex formation constants of two phosphonic acids, HEDP and ATMP, with three trivalent metallic cations, Al(III), Cr(III) and Fe(III), have been determined by acid-base titration at 25 degrees C and constant ionic strength (0.1 mol l(-1), KNO(3)), using Martell and Motekaitis' computer programs. Species distribution curves showed that all three cations are in complex form in the pH range of fresh waters (5-9). The study of different cation/ligand ratios proved that both ligands mainly form anionic soluble complexes for systems having an excess of ligand-as protonated and unprotonated forms and especially ternary complexes with HEDP. For higher metal concentrations (excess of cation), weakly soluble species of HEDP and ATMP were formed with Al(III) and Cr(III). Two insoluble complexes with ATMP have been identified by SEM/EDAX as AlH(3)X((s)) and Cr(2)X((s)). Regarding Fe(III) species, Fe(OH)(3(s)) precipitate seems to predominate in solution. PMID:18967224

  10. Synthesis procedure optimization and characterization of europium (III) tungstate nanoparticles

    NASA Astrophysics Data System (ADS)

    Rahimi-Nasrabadi, Mehdi; Pourmortazavi, Seied Mahdi; Ganjali, Mohammad Reza; Reza Banan, Ali; Ahmadi, Farhad

    2014-09-01

    Taguchi robust design as a statistical method was applied for the optimization of process parameters in order to tunable, facile and fast synthesis of europium (III) tungstate nanoparticles. Europium (III) tungstate nanoparticles were synthesized by a chemical precipitation reaction involving direct addition of europium ion aqueous solution to the tungstate reagent solved in an aqueous medium. Effects of some synthesis procedure variables on the particle size of europium (III) tungstate nanoparticles were studied. Analysis of variance showed the importance of controlling tungstate concentration, cation feeding flow rate and temperature during preparation of europium (III) tungstate nanoparticles by the proposed chemical precipitation reaction. Finally, europium (III) tungstate nanoparticles were synthesized at the optimum conditions of the proposed method. The morphology and chemical composition of the prepared nano-material were characterized by means of X-ray diffraction, scanning electron microscopy, transmission electron microscopy, FT-IR spectroscopy and fluorescence.

  11. Protein-water dynamics in antifreeze protein III activity

    NASA Astrophysics Data System (ADS)

    Xu, Yao; Bäumer, Alexander; Meister, Konrad; Bischak, Connor G.; DeVries, Arthur L.; Leitner, David M.; Havenith, Martina

    2016-03-01

    We combine Terahertz absorption spectroscopy (THz) and molecular dynamics (MD) simulations to investigate the underlying molecular mechanism for the antifreeze activity of one class of antifreeze protein, antifreeze protein type III (AFP-III) with a focus on the collective water hydrogen bond dynamics near the protein. After summarizing our previous work on AFPs, we present a new investigation of the effects of cosolutes on protein antifreeze activity by adding sodium citrate to the protein solution of AFP-III. Our results reveal that for AFP-III, unlike some other AFPs, the addition of the osmolyte sodium citrate does not affect the hydrogen bond dynamics at the protein surface significantly, as indicated by concentration dependent THz measurements. The present data, in combination with our previous THz measurements and molecular simulations, confirm that while long-range solvent perturbation is a necessary condition for the antifreeze activity of AFP-III, the local binding affinity determines the size of the hysteresis.

  12. Assessing Old Order Amish outpatients with the MCMI-III.

    PubMed

    Knabb, Joshua J; Vogt, Ronald G

    2011-05-01

    In this study, we examined Millon Clinical Multiaxial Inventory-III (MCMI-III; Millon, 2009) characteristics in an Old Order Amish outpatient sample (n = 166), with a comparison group of Old Order Amish who were not receiving mental health treatment at the time of testing (n = 80). We also graphically compared the 2 Amish groups to a non-Amish psychiatric sample in the literature. Consistent with our hypotheses, the Old Order Amish outpatients scored significantly higher than the Old Order Amish comparison group on the majority of MCMI-III scales, with mostly medium effect sizes, suggesting that the MCMI-III is a useful personality instrument in discriminating between Old Order Amish clinical and nonclinical groups. In addition, the Amish outpatients scored similar to a non-Amish psychiatric sample in the literature on most personality scales. Future MCMI-III studies with the Amish are needed to replicate and generalize our findings. PMID:21516588

  13. Methods for forming group III-arsenide-nitride semiconductor materials

    NASA Technical Reports Server (NTRS)

    Major, Jo S. (Inventor); Welch, David F. (Inventor); Scifres, Donald R. (Inventor)

    2002-01-01

    Methods are disclosed for forming Group III-arsenide-nitride semiconductor materials. Group III elements are combined with group V elements, including at least nitrogen and arsenic, in concentrations chosen to lattice match commercially available crystalline substrates. Epitaxial growth of these III-V crystals results in direct bandgap materials, which can be used in applications such as light emitting diodes and lasers. Varying the concentrations of the elements in the III-V crystals varies the bandgaps, such that materials emitting light spanning the visible spectra, as well as mid-IR and near-UV emitters, can be created. Conversely, such material can be used to create devices that acquire light and convert the light to electricity, for applications such as full color photodetectors and solar energy collectors. The growth of the III-V crystals can be accomplished by growing thin layers of elements or compounds in sequences that result in the overall lattice match and bandgap desired.

  14. Type III Radio Bursts and Microflares

    NASA Astrophysics Data System (ADS)

    Christe, S.; Krucker, S.; Arzner, K.; Lin, R. P.

    2003-05-01

    We present recent observations of microflares observed simultaneously in EUV (TRACE), radio (Nancay, Phoenix-2), and X-rays (RHESSI). During a period of 15 min on 19 July 2002 14:23-14:35 UT, RHESSI observed microflares approximately every 2 minutes. Each microflare was accompagnied by a radio Type III burst. The largest flare (14:29:25 UT) was also accompagnied by a cluster of decimetric radio spikes in the frequency range 1 to 2 GHz. In addition, FeXII (195 Å) images provided by TRACE show two jets-like emissions originating from a complex double arche structure. The centroid of the jets were found to travel at apparent speeds of ˜ 100 km s-1, consistent with observations by Shimojo et al. (1996). X-ray images show non-thermal emission (9-30 keV) from the footpoints of the TRACE arches. Strong correlation in flux amplitude is found between emissions in the radio ( ˜1340 MHz) and non-thermal X-ray (9-30 keV integrated). The event is interpreted as an anemone-jet in the model by Shibata et al. (1994). This research is supported by NASA contract NAS 5-98033.

  15. Small-satellite technology and applications III

    SciTech Connect

    Horais, B.J.

    1993-01-01

    This third conference on small-satellite applications has combined a number of significant and timely presentations on the status, in the US and on the international front, of this emerging industry. Presentations by all of the major Department of Defense activities in this field, including an overview of the ARPA CAMEO multispectral remote sensing satellite program, space activities at the Air Force Phillips Laboratory, and a space systems capabilities overview of the Naval Research Laboratory, are complemented by presentations from several international activities on their accomplishments and progress in the development of remote sensing satellite programs. For example: Spar Aerospace of Canada presented an overview of the progress they have made in establishing a space program through the use of small satellites, and the University of Surrey and Spar Aerospace provided an overview of the application of image compression schemes to imagery obtained from their UoSAT series of satellites. In addition, a number of papers were presented that summarize the state of technology in supporting activities, such as the development of the low-cost composite standardized satellite bus structures, accurate star trackers, and the application of JPEG and MPEG compression capabilities. Small-Satellite Technology and Applications III also addresses the business and cost estimating aspects of the emerging small-satellite industry as a means of increasing the overall awareness of the community in all aspects of developing a small-satellite remote sensing capability. Separate abstracts were prepared for 25 papers in this conference.

  16. The luminosity of Population III star clusters

    NASA Astrophysics Data System (ADS)

    DeSouza, Alexander L.; Basu, Shantanu

    2015-06-01

    We analyse the time evolution of the luminosity of a cluster of Population III protostars formed in the early Universe. We argue from the Jeans criterion that primordial gas can collapse to form a cluster of first stars that evolve relatively independently of one another (i.e. with negligible gravitational interaction). We model the collapse of individual protostellar clumps using non-axisymmetric numerical hydrodynamics simulations. Each collapse produces a protostar surrounded by a massive disc (i.e. Mdisc /M* ≳ 0.1), whose evolution we follow for a further 30-40 kyr. Gravitational instabilities result in the fragmentation and the formation of gravitationally bound clumps within the disc. The accretion of these fragments by the host protostar produces accretion and luminosity bursts on the order of 106 L⊙. Within the cluster, we show that a simultaneity of such events across several protostellar cluster members can elevate the cluster luminosity to 5-10 times greater than expected, and that the cluster spends ˜15 per cent of its star-forming history at these levels. This enhanced luminosity effect is particularly enabled in clusters of modest size with ≃10-20 members. In one such instance, we identify a confluence of burst events that raise the luminosity to nearly 1000 times greater than the cluster mean luminosity, resulting in L > 108 L⊙. This phenomenon arises solely through the gravitational-instability-driven episodic fragmentation and accretion that characterizes this early stage of protostellar evolution.

  17. Space Phase III - The commercial era dawns

    NASA Technical Reports Server (NTRS)

    Allnutt, R. F.

    1983-01-01

    After the 'Phase I' of space activities, the period bounded by Sputnik and Apollo, 'Phase II', has been entered, a phase in which concerns over the use and the protection of space assets which support national security predominate. However, it is only when the commercial motive becomes prominent that human activity in new regions truly prospers and enters periods of exponential growth. It is believed that there are increasing signs that such a period, called 'Space Phase III', may be coming soon. A description is presented of developments and results upon which this conclusion is based. Since 1980, there have been three developments of great importance for the future of space activities. Six highly successful flights have demonstrated that the Space Shuttle concept works. A series of Soviet missions are related to the emergence of a capability to construct and service modular space stations. Successful tests of the European Ariane 1 indicate an end to U.S. monopoly with respect to the provision of launch services to the Western World.

  18. Neptunium Binding Kinetics with Arsenazo(III)

    SciTech Connect

    Leigh R. Martin; Aaron T. Johnson; Stephen P. Mezyk

    2014-08-01

    This document has been prepared to meet FCR&D level 2 milestone M2FT-14IN0304021, “Report on the results of actinide binding kinetics with aqueous phase complexants” This work was carried out under the auspices of the Thermodynamics and Kinetics of Advanced Separations Systems FCR&D work package. The report details kinetics experiments that were performed to measure rates of aqueous phase complexation for pentavalent neptunium with the chromotropic dye Arsenazo III (AAIII). The studies performed were designed to determine how pH, ionic strength and AAIII concentration may affect the rate of the reaction. A brief comparison with hexavalent neptunium is also made. It was identified that as pH was increased the rate of reaction also increased, however increasing the ionic strength and concentration of AAIII had the opposite effect. Interestingly, the rate of reaction of Np(VI) with AAIII was found to be slower than that of the Np(V) reaction.

  19. Joint Eglin Acoustic Week III Data Report

    NASA Technical Reports Server (NTRS)

    Watts, Michael E.; Conner, David A.; Smith, Charles D.

    2010-01-01

    A series of three flight tests have been conducted at an Eglin Air Force Base remote test range located in the Florida panhandle. The first was the "Acoustics Week" flight test conducted in September 2003. The second was the NASA Heavy Lift Rotorcraft Acoustic Flight Test conducted in October-November 2005. The most recent was the Eglin Acoustic Week III test conducted in August-September 2007. This series of tests acquired acoustic data for a number of rotary and fixed wing aircraft and are used to generate noise semi-spheres used in predicting the acoustic footprint for prescribed flight operations. This extensive database can be used to determine the impact of flight operations on communities around a terminal area as well as for prediction code validations. Another valuable use of the semi-spheres is determining the long-range propagation of noise for civilian and military purposes. This paper describes the third test in this series. Data described in this report were acquired during testing of the MD-902 and Mi-8M aircraft. In addition, data acquired during a set of atmospheric propagation tests is also described.

  20. Stellar populations in Active Galactic Nuclei III

    NASA Astrophysics Data System (ADS)

    Boisson, C.; Joly, M.; Pelat, D.; Ward, M. J.

    2004-12-01

    In this paper we apply the stellar population synthesis method previously described in Boisson et al. (\\cite{Boisson2000}) to five more AGN. The analysis of these new data strengthen our previous conclusions: i) homogeneity of the stellar population within a class of nuclear activity regardless of the morphological type of the host galaxy; ii) populations within the nuclear regions of LINERs and Seyfert 2s are different: LINERs have a very old metal-rich population while in the Seyfert 2s a contribution of a weak burst of star formation is observed together with the old high metallicity component; iii) in the circum-nuclar region (200 pc ≤D≤1 kpc) of all the active galaxies in our sample, except for NGC 2992, we detect an old burst of star formation (0.2-1 Gyr),which is contrary to what is observed in normal galaxies. We note that the broad OIλ8446 Å emission line detected in the spectrum of the nucleus of NGC 2992 confirms its classification as a Seyfert 1. Based on observations collected at the New Technology Telescope of the European Southern Observatory, La Silla, Chile.

  1. ADVANCED HYBRID PARTICULATE COLLECTOR - PHASE III

    SciTech Connect

    Stanley J. Miller; Ye Zhuang; Michael E. Collings; Michelle R. Olderbak

    2000-10-01

    A new concept in particulate control, called an advanced hybrid particulate collector (AHPC), is being developed under funding from the U.S. Department of Energy. The AHPC combines the best features of electrostatic precipitators (ESPs) and baghouses in a unique configuration. The AHPC concept consists of a combination of fabric filtration and electrostatic precipitation in the same housing, providing major synergism between the two collection methods, both in the particulate collection step and in the transfer of dust to the hopper. The AHPC provides ultrahigh collection efficiency, overcoming the problem of excessive fine-particle emission with conventional ESPs, and it solves the problem of reentrainment and re-collection of dust in conventional baghouses. In Phase II, a 2.5-MW-scale AHPC was designed, constructed, installed, and tested at the Big Stone power station. For Phase III, further testing of an improved version of the 2.5-MW-scale AHPC at the Big Stone power station is being conducted to facilitate commercialization of the AHPC technology.

  2. Structural Characterization of Sm(III)(EDTMP).

    PubMed

    Yang, Y; Pushie, M J; Cooper, D M L; Doschak, M R

    2015-11-01

    Samarium-153 ethylenediamine-N,N,N',N'-tetrakis(methylenephosphonic acid) ((153)Sm-EDTMP, or samarium lexidronam), also known by its registered trademark name Quadramet, is an approved therapeutic radiopharmaceutical used in the palliative treatment of painful bone metastases. Typically, patients with prostate, breast, or lung cancer are most likely to go on to require bone pain palliation treatment due to bone metastases. Sm(EDTMP) is a bone-seeking drug which accumulates on rapidly growing bone, thereby delivering a highly region-specific dose of radiation, chiefly through β particle emission. Even with its widespread clinical use, the structure of Sm(EDTMP) has not yet been characterized at atomic resolution, despite attempts to crystallize the complex. Herein, we prepared a 1:1 complex of the cold (stable isotope) of Sm(EDTMP) under alkaline conditions and then isolated and characterized the complex using conventional spectroscopic techniques, as well as with extended X-ray absorption fine structure (EXAFS) spectroscopy and density functional structure calculations, using natural abundance Sm. We present the atomic resolution structure of [Sm(III)(EDTMP)-8H](5-) for the first time, supported by the EXAFS data and complementary spectroscopic techniques, which demonstrate that the samarium coordination environment in solution is in agreement with the structure that has long been conjectured. PMID:26437889

  3. Magnetic Fields in Population III Star Formation

    SciTech Connect

    Turk, Matthew J.; Oishi, Jeffrey S.; Abel, Tom; Bryan, Greg

    2012-02-22

    We study the buildup of magnetic fields during the formation of Population III star-forming regions, by conducting cosmological simulations from realistic initial conditions and varying the Jeans resolution. To investigate this in detail, we start simulations from identical initial conditions, mandating 16, 32 and 64 zones per Jeans length, and studied the variation in their magnetic field amplification. We find that, while compression results in some amplification, turbulent velocity fluctuations driven by the collapse can further amplify an initially weak seed field via dynamo action, provided there is sufficient numerical resolution to capture vortical motions (we find this requirement to be 64 zones per Jeans length, slightly larger than, but consistent with previous work run with more idealized collapse scenarios). We explore saturation of amplification of the magnetic field, which could potentially become dynamically important in subsequent, fully-resolved calculations. We have also identified a relatively surprising phenomena that is purely hydrodynamic: the higher-resolved simulations possess substantially different characteristics, including higher infall-velocity, increased temperatures inside 1000 AU, and decreased molecular hydrogen content in the innermost region. Furthermore, we find that disk formation is suppressed in higher-resolution calculations, at least at the times that we can follow the calculation. We discuss the effect this may have on the buildup of disks over the accretion history of the first clump to form as well as the potential for gravitational instabilities to develop and induce fragmentation.

  4. Solvation structure and thermodynamics for Pr(III), Nd(III) and Dy(III) complexes in ionic liquids evaluated by Raman spectroscopy and DFT calculation

    NASA Astrophysics Data System (ADS)

    Kuribara, Keita; Matsumiya, Masahiko; Tsunashima, Katsuhiko

    2016-12-01

    The coordination states of trivalent praseodymium, neodymium, and dysprosium complexes in the ionic liquid, triethyl-n-pentylphosphonium bis(trifluoromethyl-sulfonyl) amide ([P2225][TFSA]) were investigated by Raman spectroscopy. The effect of the concentration of rare earth ions on the Raman spectra was investigated, ranging from 0.23 to 0.45 mol kg-1 of Pr(III), Nd(III), and Dy(III) in [P2225][TFSA]. Based on a conventional analysis, the solvation numbers, n, of Pr(III), Nd(III), and Dy(III) in [P2225][TFSA] were determined to be 4.99, 5.01, and 5.00 at 298 K and 5.04, 5.06, and 5.07 at 373 K, respectively. Thermodynamic properties such as ΔisoG, ΔisoH, and ΔisoS for the isomerism of [TFSA]- from trans- to cis-coordinated isomer in the bulk and the first solvation sphere of the central RE3+ (RE = Pr, Nd, and Dy) cation in [P2225][TFSA] were evaluated from the temperature dependence of the Raman bands, measured at temperatures ranging from 298 to 398 K. Regarding the bulk properties, ΔisoG(bulk), ΔisoH(bulk), and TΔisoS(bulk) at 298 K were found to be -1.06, 6.86, and 7.92 kJ mol-1, respectively. The trans-[TFSA]- was a dominant contributor to the enthalpy, as shown by the positive value of ΔisoH(bulk). The value of TΔisoS(bulk) was slightly larger than that of ΔisoH(bulk), and cis-[TFSA]- was, therefore, entropy-controlled in [P2225][TFSA]. In contrast, in the first solvation sphere of the RE3+ cation, ΔisoH(RE) became remarkably negative, suggesting that cis-[TFSA]- isomers were stabilized by enthalpic contributions. Furthermore, ΔisoH(RE) contributed to the remarkable decrease in ΔisoG(RE), and this result clearly indicates that cis-[TFSA]- conformers bound to RE3+ cations are the preferred coordination state of [RE(III)(cis-TFSA)5]2- in [P2225][TFSA]. Moreover, optimized geometries and binding energies of [Pr(III)(cis-TFSA)5]2-, [Nd(III)(cis-TFSA)5]2-, and [Dy(III)(cis-TFSA)5]2- clusters were also investigated by DFT calculations using the ADF

  5. Systematic Study of a Family of Butterfly-Like {M2Ln2} Molecular Magnets (M = Mg(II), Mn(III), Co(II), Ni(II), and Cu(II); Ln = Y(III), Gd(III), Tb(III), Dy(III), Ho(III), and Er(III)).

    PubMed

    Moreno Pineda, Eufemio; Chilton, Nicholas F; Tuna, Floriana; Winpenny, Richard E P; McInnes, Eric J L

    2015-06-15

    A family of 3d-4f [M(II)2Ln(III)2(μ3-OH)2(O2C(t)Bu)10](2-) "butterflies" (where M(II) = Mg, Co, Ni, and Cu; Ln(III) = Y, Gd, Tb, Dy, Ho, and Er) and [Mn(III)2Ln(III)2(μ3-O)2(O2C(t)Bu)10](2-) molecules (where Ln(III) = Y, Gd, Tb, Dy, Ho, and Er) has been synthesized and characterized through single-crystal X-ray diffraction, SQUID magnetometry, and ab initio calculations. All dysprosium- and some erbium-containing tetramers showed frequency-dependent maxima in the out-of-phase component of the susceptibility associated with slow relaxation of magnetization, and hence, they are single-molecule magnets (SMMs). AC susceptibility measurements have shown that the SMM behavior is entirely intrinsic to the Dy and Er sites and the magnitude of the energy barrier is influenced by the interactions between the 4f and the 3d metal. A trend is observed between the strength of the 3d-4f exchange interaction between and the maximum observed in the χ″M(T). PMID:26016421

  6. Heteronuclear Ir(III)-Ln(III) Luminescent Complexes: Small-Molecule Probes for Dual Modal Imaging and Oxygen Sensing.

    PubMed

    Jana, Atanu; Crowston, Bethany J; Shewring, Jonathan R; McKenzie, Luke K; Bryant, Helen E; Botchway, Stanley W; Ward, Andrew D; Amoroso, Angelo J; Baggaley, Elizabeth; Ward, Michael D

    2016-06-01

    Luminescent, mixed metal d-f complexes have the potential to be used for dual (magnetic resonance imaging (MRI) and luminescence) in vivo imaging. Here, we present dinuclear and trinuclear d-f complexes, comprising a rigid framework linking a luminescent Ir center to one (Ir·Ln) or two (Ir·Ln2) lanthanide metal centers (where Ln = Eu(III) and Gd(III), respectively). A range of physical, spectroscopic, and imaging-based properties including relaxivity arising from the Gd(III) units and the occurrence of Ir(III) → Eu(III) photoinduced energy-transfer are presented. The rigidity imposed by the ligand facilitates high relaxivities for the Gd(III) complexes, while the luminescence from the Ir(III) and Eu(III) centers provide luminescence imaging capabilities. Dinuclear (Ir·Ln) complexes performed best in cellular studies, exhibiting good solubility in aqueous solutions, low toxicity after 4 and 18 h, respectively, and punctate lysosomal staining. We also demonstrate the first example of oxygen sensing in fixed cells using the dyad Ir·Gd, via two-photon phosphorescence lifetime imaging (PLIM). PMID:27219675

  7. Ditopic CMPO-pillar[5]arenes as unique receptors for efficient separation of americium(III) and europium(III).

    PubMed

    Fang, Yuyu; Yuan, Xiangyang; Wu, Lei; Peng, Zhiyong; Feng, Wen; Liu, Ning; Xu, Dingguo; Li, Shoujian; Sengupta, Arijit; Mohapatra, Prasanta K; Yuan, Lihua

    2015-03-11

    A unique host-guest recognition process involving a new class of homoditopic CMPO-pillar[5]arenes and lanthanides was revealed to proceed in a stepwise manner, and correlated with the efficient separation of americium(III) and europium(III) under acidic feed conditions. PMID:25671799

  8. Iron(III) hydroxide-loaded coral limestone as an adsorbent for arsenic(III) and arsenic (V)

    SciTech Connect

    Maeda, Shigeru; Ohki, Akira; Saikoji, Shunsuke; Naka, Kensuke )

    1992-04-01

    Trace levels of As(III) and As(V) in aqueous media were effectively adsorbed onto a coral limestone loaded by Fe(OH){sub 3}. The adsorption of As(III) was almost comparable to that of As(V). The adsorption of As(III) and As(V) was almost independent of the pH of the aqueous phase (pH range: 3-10) because of a self-buffering effect of the coral. The addition of such anions as chloride, nitrate, sulfate, and acetate in the aqueous phase did not significantly affect the adsorption of As(III), whereas the addition of phosphate brought about a great decrease in the adsorption. The arsenic adsorption was effectively applied to the column method. Unloaded coral itself was effective as an adsorbent for As(V) when Fe(III) coexisted in the aqueous solutions.

  9. III-V semiconductor devices integrated with silicon III-V semiconductor devices integrated with silicon

    NASA Astrophysics Data System (ADS)

    Hopkinson, Mark; Martin, Trevor; Smowton, Peter

    2013-09-01

    The integration of III-V semiconductor devices with silicon is one of the most topical challenges in current electronic materials research. The combination has the potential to exploit the unique optical and electronic functionality of III-V technology with the signal processing capabilities and advanced low-cost volume production techniques associated with silicon. Key industrial drivers include the use of high mobility III-V channel materials (InGaAs, InAs, InSb) to extend the performance of Si CMOS, the unification of electronics and photonics by combining photonic components (GaAs, InP) with a silicon platform for next-generation optical interconnects and the exploitation of large-area silicon substrates and high-volume Si processing capabilities to meet the challenges of low-cost production, a challenge which is particularly important for GaN-based devices in both power management and lighting applications. The diverse nature of the III-V and Si device approaches, materials technologies and the distinct differences between industrial Si and III-V processing have provided a major barrier to integration in the past. However, advances over the last decade in areas such as die transfer, wafer fusion and epitaxial growth have promoted widespread renewed interest. It is now timely to bring some of these topics together in a special issue covering a range of approaches and materials providing a snapshot of recent progress across the field. The issue opens a paper describing a strategy for the epitaxial integration of photonic devices where Kataria et al describe progress in the lateral overgrowth of InP/Si. As an alternative, Benjoucef and Reithmaier report on the potential of InAs quantum dots grown direct onto Si surfaces whilst Sandall et al describe the properties of similar InAs quantum dots as an optical modulator device. As an alternative to epitaxial integration approaches, Yokoyama et al describe a wafer bonding approach using a buried oxide concept, Corbett

  10. EGFRvIII and c-Met pathway inhibitors synergize against PTEN-null/EGFRvIII+ glioblastoma xenografts

    PubMed Central

    Lal, Bachchu; Goodwin, Courtney R.; Sang, Yingying; Foss, Catherine A.; Cornet, Kathrine; Muzamil, Sameena; Pomper, Martin G.; Kim, Jin; Laterra, John

    2010-01-01

    Receptor tyrosine kinase (RTK) systems, such as hepatocyte growth factor (HGF) and its receptor c-Met, and EGFR, are responsible for the malignant progression of multiple solid tumors. Recent research shows that these RTK systems co-modulate overlapping and dynamically adaptable oncogenic downstream signaling pathways. This paper investigates how EGFRvIII, a constitutively active EGFR deletion mutant, alters tumor growth and signaling responses to RTK inhibition in PTEN-null/HGF+/c-Met+ glioma xenografts. We show that a neutralizing anti-HGF mAb (L2G7) potently inhibits tumor growth and the activation of Akt and MAPK in PTEN-null/HGF+/c-Met+/EGFRvIII−U87 glioma xenografts (U87wt). Isogenic EGFRvIII+ U87 xenografts (U87-EGFRvIII), which grew 5-times more rapidly than U87-wt xenografts, were unresponsive to EGFRvIII inhibition by erlotinib and were only minimally responsive to anti-HGF mAb. EGFRvIII-expression diminished the magnitude of Akt inhibition and completely prevented MAPK inhibition by L2G7. Despite the lack of response to L2G7 or erlotinib as single agents, their combination synergized to produce substantial anti-tumor effects (inhibited tumor cell proliferation, enhanced apoptosis, arrested tumor growth, prolonged animal survival), against subcutaneous and orthotopic U87-EGFRvIII xenografts. The dramatic response to combining HGF:c-Met and EGFRvIII pathway inhibitors in U87-EGFRvIII xenografts occurred in the absence of Akt and MAPK inhibition. These findings show that combining c-Met and EGFRvIII pathway inhibitors can generate potent anti-tumor effects in PTEN-null tumors. They also provide insights into how EGFRvIII and c-Met may alter signaling networks and reveal the potential limitations of certain biochemical biomarkers to predict the efficacy of RTK inhibition in genetically diverse cancers. PMID:19584231

  11. Specific Reagent for Cr(III): Imaging Cellular Uptake of Cr(III) in Hct116 Cells and Theoretical Rationalization.

    PubMed

    Ali, Firoj; Saha, Sukdeb; Maity, Arunava; Taye, Nandaraj; Si, Mrinal Kanti; Suresh, E; Ganguly, Bishwajit; Chattopadhyay, Samit; Das, Amitava

    2015-10-15

    A new rhodamine-based reagent (L1), trapped inside the micellar structure of biologically benign Triton-X 100, could be used for specific recognition of Cr(III) in aqueous buffer medium having physiological pH. This visible light excitable reagent on selective binding to Cr(III) resulted in a strong fluorescence turn-on response with a maximum at ∼583 nm and tail of that luminescence band extended until 650 nm, an optical response that is desired for avoiding the cellular autofluorescence. Interference studies confirm that other metal ions do not interfere with the detection process of Cr(III) in aqueous buffer medium having pH 7.2. To examine the nature of binding of Cr(III) to L1, various spectroscopic studies are performed with the model reagent L2, which tend to support Cr(III)-η(2)-olefin π-interactions involving two olefin bonds in molecular probe L1. Computational studies are also performed with another model reagent LM to examine the possibility of such Cr(III)-η(2)-olefin π-interactions. Presumably, polar functional groups of the model reagent LM upon coordination to the Cr(III) center effectively reduce the formal charge on the metal ion and this is further substantiated by results of the theoretical studies. This assembly is found to be cell membrane permeable and shows insignificant toxicity toward live colon cancer cells (Hct116). Confocal laser scanning microscopic studies further revealed that the reagent L1 could be used as an imaging reagent for detection of cellular uptake of Cr(III) in pure aqueous buffer medium by Hct116 cells. Examples of a specific reagent for paramagnetic Cr(III) with luminescence ON response are scanty in the contemporary literature. This ligand design helped us in achieving the turn on response by utilizing the conversion from spirolactam to an acyclic xanthene form on coordination to Cr(III). PMID:26390369

  12. Adsorptive separation of rhodium(III) using Fe(III)-templated oxine type of chemically modified chitosan

    SciTech Connect

    Alam, M.S.; Inoue, Katsutoshi; Yoshizuka, Kazuharu; Ishibashi, Hideaki

    1998-03-01

    The oxine type of chemically modified chitosan was prepared by the template crosslinking method using Fe(III) as a template ion. Batchwise adsorption of rhodium(III) on this chemically modified chitosan was examined from chloride media in the absence and presence of a large amount of tin(II). It was observed that the Fe(III)-templated oxine type of chemically modified chitosan shows better performance for rhodium adsorption than that of the original chitosan. When Sn(II) is absent from the solution, Rh(III) is hardly adsorbed on the modified chitosan and the order of selectivity of the adsorption of Rh(III), Pt(IV), and Cu(II) was found to be Pt(IV) > Cu(II) {approx} Rh(III). On the other hand, adsorption of rhodium is significantly increased in the presence of Sn(II) and the selectivity order of the adsorption was drastically changed to Rh(III) > Pt(IV) {much_gt} Cu(II), which ensures selective separation of Rh(III) from their mixture. Adsorption of Rh(III) increases with an increase in the concentration of Sn(II) in the aqueous solution, and maximum adsorption is achieved at a molar ratio, [Sn]/[Rh], of >6. The adsorption of Rh(III) decreases at a high concentration of hydrochloric acid. The maximum adsorption capacity was evaluated to be 0.92 mol/kg-dry adsorbent. Stripping tests of rhodium from the loaded chemically modified chitosan were carried out using different kinds of stripping agents containing some oxidizing agent. The maximum stripping of rhodium under these experimental conditions was found to be 72.5% by a single contact with 0.5 M HCl + 8 M HNO{sub 3}.

  13. 40 CFR Table 2 to Subpart III of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart III

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (40 CFR Part 63, Subpart A) to Subpart III 2 Table 2 to Subpart III of Part 63 Protection of... Hazardous Air Pollutants for Flexible Polyurethane Foam Production Pt. 63, Subpt. III, Table 2 Table 2 to Subpart III of Part 63—Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart...

  14. 40 CFR Table 2 to Subpart III of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart III

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (40 CFR Part 63, Subpart A) to Subpart III 2 Table 2 to Subpart III of Part 63 Protection of... Hazardous Air Pollutants for Flexible Polyurethane Foam Production Pt. 63, Subpt. III, Table 2 Table 2 to Subpart III of Part 63—Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart...

  15. Group-III Nitride Field Emitters

    NASA Technical Reports Server (NTRS)

    Bensaoula, Abdelhak; Berishev, Igor

    2008-01-01

    Field-emission devices (cold cathodes) having low electron affinities can be fabricated through lattice-mismatched epitaxial growth of nitrides of elements from group III of the periodic table. Field emission of electrons from solid surfaces is typically utilized in vacuum microelectronic devices, including some display devices. The present field-emission devices and the method of fabricating them were developed to satisfy needs to reduce the cost of fabricating field emitters, make them compatible with established techniques for deposition of and on silicon, and enable monolithic integration of field emitters with silicon-based driving circuitry. In fabricating a device of this type, one deposits a nitride of one or more group-III elements on a substrate of (111) silicon or other suitable material. One example of a suitable deposition process is chemical vapor deposition in a reactor that contains plasma generated by use of electron cyclotron resonance. Under properly chosen growth conditions, the large mismatch between the crystal lattices of the substrate and the nitride causes strains to accumulate in the growing nitride film, such that the associated stresses cause the film to crack. The cracks lie in planes parallel to the direction of growth, so that the growing nitride film becomes divided into microscopic growing single-crystal columns. The outer ends of the fully-grown columns can serve as field-emission tips. By virtue of their chemical compositions and crystalline structures, the columns have low work functions and high electrical conductivities, both of which are desirable for field emission of electrons. From examination of transmission electron micrographs of a prototype device, the average column width was determined to be about 100 nm and the sharpness of the tips was determined to be characterized by a dimension somewhat less than 100 nm. The areal density of the columns was found to about 5 x 10(exp 9)/sq cm . about 4 to 5 orders of magnitude

  16. PREFACE: Nuclear Physics in Astrophysics III

    NASA Astrophysics Data System (ADS)

    Bemmerer, D.; Grosse, E.; Junghans, A. R.; Schwengner, R.; Wagner, A.

    2008-01-01

    The Europhysics Conference `Nuclear Physics in Astrophysics III' (NPA3) took place from 26 31 March 2007 in Dresden, Germany, hosted by Forschungszentrum Dresden-Rossendorf. The present special issue of Journal of Physics G: Nuclear and Particle Physics contains all peer-reviewed contributions to the proceedings of this conference. NPA3 is the third conference in the Nuclear Physics in Astrophysics series of conferences devoted to the interplay between nuclear physics and astrophysics. The first and second editions of the series were held in 2002 and 2005 in Debrecen, Hungary. NPA3 has been organized under the auspices of the Nuclear Physics Board of the European Physical Society as its XXI Divisional Conference. The conference marks the 50th anniversary of the landmark paper B2FH published in 1957 by E M Burbidge, G R Burbidge, W A Fowler and F Hoyle. A public lecture by Claus Rolfs (Ruhr-Universität Bochum, Germany) commemorated the progress achieved since 1957. NPA3 aimed to bring together experimental and theoretical nuclear physicists, astrophysicists and astronomers to address the important part played by nuclear physics in current astrophysical problems. A total of 130 participants from 71 institutions in 26 countries attended the conference, presenting 33 invited and 38 contributed talks and 25 posters on six subject areas. The astrophysical motivation and the nuclear tools employed to address it are highlighted by the titles of the subject areas: Big Bang Nucleosynthesis Stellar Nucleosynthesis and Low Cross Section Measurement Explosive Nucleosynthesis and Nuclear Astrophysics with Photons Nuclei far from Stability and Radioactive Ion Beams Dense Matter in Neutron Stars and Relativistic Nuclear Collisions Neutrinos in Nuclear Astrophysics The presentations and discussions proved that Nuclear Astrophysics is a truly interdisciplinary subject. The remarkable progress in astronomical observations achieved in recent years is matched by advances in

  17. Carrier dynamics in III-nitride semiconductors

    NASA Astrophysics Data System (ADS)

    Liu, Kai

    In the past decade, III-nitride semiconductors had a considerable impact in solid state lighting and high speed high power electronics. As technology develops, high Al content III-nitride semiconductors lead the edge of research. It opens the door to many applications especially portable ones: from homeland security, bio-analytical, medical diagnostic, air and water disinfection, sterilization, chemical sensing systems, non-line-of-sight (NLOS) communications, to high-density optical data storage. In this thesis, we first study GaN epilayers, as well as more complicate high Al content AlGaN/AlGaN MQW structures used as active media for deep UV LEDs. We theoretically study the photoluminescence (PL) dynamics in high quality GaN epilayers by establishing a new decay model. In our model, surface recombination, diffusion, and re-absorption are taken into account. Our model is in excellent agreement with experimental data obtained by time-resolved PL. Our results show that the carrier diffusion and surface recombination play key roles in the PL decay. For high Al content AlGaN/AlGaN MQW structures, we first present the investigation of built-in electric fields in AlxGa1-xN/Al yGa1-yN MQWs embedded into p-i-n structure by using photoluminescence experiments. By comparison of the Stark shifts induced by the p-i-n structure and by photo-excited free carrier screening, we evaluate the intrinsic electric field induced by piezoelectric and spontaneous polarizations. Furthermore we investigate carrier dynamics in sets of identically grown Al0.35Ga0.65N/Al 0.49Ga0.51N MQW structures with well widths varying from 1.65 to 5.0 nm by TR-PL and LITG techniques. We observed screening of the built-in electric field by free non-equilibrium carriers and localization governed PL kinetics at different decay stages. A decrease of carrier lifetime with increasing well width is observed and attributed to the carrier localization occurring due to well width fluctuations of the quantum well

  18. Comparison of fatigue crack propagation in Modes I and III

    SciTech Connect

    Ritchie, R.O.

    1985-06-01

    The propagation behavior of fatigue cracks in Mode III (anti-plane shear), measured under cyclic torsion, is described and compared with more commonly encountered behavior under Mode I (tensile opening) loads. It is shown that a unique, global characterization of Mode III growth rates, akin to the Paris ''law'' in Mode I, is only possible if characterizating parameters appropriate to large-scale yielding are employed and allowance is made for crack tip shielding from sliding crack surface interference (i.e., friction and abrasion) between mating fracture surfaces. Based on the crack tip stress and deformation fields for Mode III stationary cracks, the cyclic crack tip displacement, (..delta..CTD/sub III/, and plastic strain intensity range ..delta..GAMMA/sub III/, have been proposed and are found to provide an adequate description of behavior in a range of steels, provided crack surface interference is minimized. The magnitude of this interference, which is somewhat analogous to crack closure in Mode I, is further examined in the light of the complex fractography of torsional fatigue failures and the question of a ''fatigue threshold'' for Mode III crack growth. Finally, micro-mechanical models for cyclic crack extension in anti-plane shear are briefly described, and the contrasting behavior between Mode III and Mode I cracks subjected to simple variable amplitude spectra is examined in terms of the differing role of crack tip blunting and closure in influencing shear, as opposed to tensile opening, modes of crack growth.

  19. Targeting ApoC-III to Reduce Coronary Disease Risk.

    PubMed

    Khetarpal, Sumeet A; Qamar, Arman; Millar, John S; Rader, Daniel J

    2016-09-01

    Triglyceride-rich lipoproteins (TRLs) are causal contributors to the risk of developing coronary artery disease (CAD). Apolipoprotein C-III (apoC-III) is a component of TRLs that elevates plasma triglycerides (TGs) through delaying the lipolysis of TGs and the catabolism of TRL remnants. Recent human genetics approaches have shown that heterozygous loss-of-function mutations in APOC3, the gene encoding apoC-III, lower plasma TGs and protect from CAD. This observation has spawned new interest in therapeutic efforts to target apoC-III. Here, we briefly review both currently available as well as developing therapies for reducing apoC-III levels and function to lower TGs and cardiovascular risk. These therapies include existing options including statins, fibrates, thiazolidinediones, omega-3-fatty acids, and niacin, as well as an antisense oligonucleotide targeting APOC3 currently in clinical development. We review the mechanisms of action by which these drugs reduce apoC-III and the current understanding of how reduction in apoC-III may impact CAD risk. PMID:27443326

  20. Microbial and geochemical controls on dissimilatory Fe(III) reduction

    SciTech Connect

    Urrutia, M.M.; Roden, E.E.; Zachara, J.M.

    1995-12-31

    This study investigates the influence of coating bacterial oxide surfaces with Fe(II) on the rate and extent of Fe(III) reduction. Limitations imposed by the microorganisms themselves on the rate and extent of reduction of solid phase Fe(III) were also examined. Shewanella alga (strain BrY) cells were coated with Fe(II) and used as inocula for Fe(III) reduction experiments. The coating considerably slowed down the initial rate of Fe(III) reduction in comparison with uncoated cells, proceeding at a rate similar to that exhibited by uncoated cells at the latest stages of growth. Furthermore, the presence of chelators (EDTA and NTA) with a high affinity for Fe(II) was related to further reduction of medium surface area Goethite (MSA Gt) by BrY, which supports the idea of cessation of reduction by occlusion of the active surfaces. Reinoculation experiments, in which fresh cells were added after completion of a whole cell cycle, showed further reduction of Fe(III) with either MSA Gt, or hematite, high surface area and MSA Gt. Together, findings support the hypothesis that the binding, and eventual coating, of Fe(III) oxide and bacterial surfaces with Fe(II) is involved in the slowdown and eventual cessation of Fe(III) reduction within a cell growth cycle.

  1. Retrotransposon profiling of RNA polymerase III initiation sites.

    PubMed

    Qi, Xiaojie; Daily, Kenneth; Nguyen, Kim; Wang, Haoyi; Mayhew, David; Rigor, Paul; Forouzan, Sholeh; Johnston, Mark; Mitra, Robi David; Baldi, Pierre; Sandmeyer, Suzanne

    2012-04-01

    Although retroviruses are relatively promiscuous in choice of integration sites, retrotransposons can display marked integration specificity. In yeast and slime mold, some retrotransposons are associated with tRNA genes (tDNAs). In the Saccharomyces cerevisiae genome, the long terminal repeat retrotransposon Ty3 is found at RNA polymerase III (Pol III) transcription start sites of tDNAs. Ty1, 2, and 4 elements also cluster in the upstream regions of these genes. To determine the extent to which other Pol III-transcribed genes serve as genomic targets for Ty3, a set of 10,000 Ty3 genomic retrotranspositions were mapped using high-throughput DNA sequencing. Integrations occurred at all known tDNAs, two tDNA relics (iYGR033c and ZOD1), and six non-tDNA, Pol III-transcribed types of genes (RDN5, SNR6, SNR52, RPR1, RNA170, and SCR1). Previous work in vitro demonstrated that the Pol III transcription factor (TF) IIIB is important for Ty3 targeting. However, seven loci that bind the TFIIIB loader, TFIIIC, were not targeted, underscoring the unexplained absence of TFIIIB at those sites. Ty3 integrations also occurred in two open reading frames not previously associated with Pol III transcription, suggesting the existence of a small number of additional sites in the yeast genome that interact with Pol III transcription complexes. PMID:22287102

  2. Assessment of Sleep in Children with Mucopolysaccharidosis Type III

    PubMed Central

    Mahon, Louise Victoria; Lomax, Michelle; Grant, Sheena; Cross, Elaine; Hare, Dougal Julian; Wraith, James Ed; Jones, Simon; Bigger, Brian; Langford-Smith, Kia; Canal, Maria

    2014-01-01

    Sleep disturbances are prevalent in mucopolysaccharidosis Type III (MPS III), yet there is a lack of objective, ecologically valid evidence detailing sleep quantity, quality or circadian system. Eight children with MPS III and eight age-matched typically developing children wore an actigraph for 7–10 days/nights. Saliva samples were collected at three time-points on two separate days, to permit analysis of endogenous melatonin levels. Parents completed a sleep questionnaire and a daily sleep diary. Actigraphic data revealed that children with MPS III had significantly longer sleep onset latencies and greater daytime sleep compared to controls, but night-time sleep duration did not differ between groups. In the MPS III group, sleep efficiency declined, and sleep onset latency increased, with age. Questionnaire responses showed that MPS III patients had significantly more sleep difficulties in all domains compared to controls. Melatonin concentrations showed an alteration in the circadian system in MPS III, which suggests that treatment for sleep problems should attempt to synchronise the sleep-wake cycle to a more regular pattern. Actigraphy was tolerated by children and this monitoring device can be recommended as a measure of treatment success in research and clinical practice. PMID:24504123

  3. Clinical management of grade III oligodendroglioma.

    PubMed

    Simonetti, G; Gaviani, P; Botturi, A; Innocenti, A; Lamperti, E; Silvani, A

    2015-01-01

    Oligodendrogliomas represent the third most common type of glioma, comprising 4%-15% of all gliomas and can be classified by degree of malignancy into grade II and grade III, according to WHO classification. Only 30% of oligodendroglial tumors have anaplastic characteristics. Anaplastic oligodendroglioma (AO) is often localized as a single lesion in the white matter and in the cortex, rarely in brainstem or spinal cord. The management of AO is deeply changed in the recent years. Maximal safe surgical resection followed by radiotherapy (RT) was considered as the standard of care since paramount findings regarding molecular aspects, in particular co-deletion of the short arm of chromosome 1 and the long arm of chromosome 19, revealed that these subsets of AO, benefit in terms of overall survival (OS) and progression-free survival (PFS), from the addition of chemotherapy to RT. Allelic losses of chromosomes 1p and 19q occur in 50%-70% of both low-grade and anaplastic tumors, representing a strong prognostic factor and a powerful predictor of prolonged survival. Several other molecular markers have potential clinical significance as IDH1 mutations, confirming the strong prognostic role for OS. Malignant brain tumors negatively impacts on patients' quality of life. Seizures, visual impairment, headache, and cognitive disorders can be present. Moreover, chemotherapy and RT have important side effects. For these reasons, "health-related quality of life" is becoming a topic of growing interest, investigating on physical, mental, emotional, and social well-being. Understanding the impact of medical treatment on health-related quality of life will probably have a growing effect both on health care strategies and on patients. PMID:26251628

  4. Antimony Based III-V Thermophotovoltaic Devices

    SciTech Connect

    CA Wang

    2004-06-09

    Antimony-based III-V thermophotovoltaic (TPV) cells are attractive converters for systems with low radiator temperature around 1100 to 1700 K, since these cells potentially can be spectrally matched to the thermal source. Cells under development include GaSb and the lattice-matched GaInAsSb/GaSb and InPAsSb/InAs quaternary systems. GaSb cell technology is the most mature, owing in part to the relative ease in preparation of the binary alloy compared to quaternary GaInAsSb and InPAsSb alloys. Device performance of 0.7-eV GaSb cells exceeds 90% of the practical limit. GaInAsSb TPV cells have been the primary focus of recent research, and cells with energy gap E{sub g} ranging from {approx}0.6 to 0.49 eV have been demonstrated. Quantum efficiency and fill factor approach theoretical limits. Open-circuit voltage factor is as high as 87% of the practical limit for the higher-E{sub g} cells, but degrades to below 80% with decreasing E{sub g} of the alloy, which might be due to Auger recombination. InPAsSb cells are the least studied, and a cell with E{sub g} = 0.45-eV has extended spectral response out to 4.3 {micro}m. This paper briefly reviews the main contributions that have been made for antimonide-based TPV cells, and suggests additional studies for further performance enhancements.

  5. Clinical management of grade III oligodendroglioma

    PubMed Central

    Simonetti, G; Gaviani, P; Botturi, A; Innocenti, A; Lamperti, E; Silvani, A

    2015-01-01

    Oligodendrogliomas represent the third most common type of glioma, comprising 4%–15% of all gliomas and can be classified by degree of malignancy into grade II and grade III, according to WHO classification. Only 30% of oligodendroglial tumors have anaplastic characteristics. Anaplastic oligodendroglioma (AO) is often localized as a single lesion in the white matter and in the cortex, rarely in brainstem or spinal cord. The management of AO is deeply changed in the recent years. Maximal safe surgical resection followed by radiotherapy (RT) was considered as the standard of care since paramount findings regarding molecular aspects, in particular co-deletion of the short arm of chromosome 1 and the long arm of chromosome 19, revealed that these subsets of AO, benefit in terms of overall survival (OS) and progression-free survival (PFS), from the addition of chemotherapy to RT. Allelic losses of chromosomes 1p and 19q occur in 50%–70% of both low-grade and anaplastic tumors, representing a strong prognostic factor and a powerful predictor of prolonged survival. Several other molecular markers have potential clinical significance as IDH1 mutations, confirming the strong prognostic role for OS. Malignant brain tumors negatively impacts on patients’ quality of life. Seizures, visual impairment, headache, and cognitive disorders can be present. Moreover, chemotherapy and RT have important side effects. For these reasons, “health-related quality of life” is becoming a topic of growing interest, investigating on physical, mental, emotional, and social well-being. Understanding the impact of medical treatment on health-related quality of life will probably have a growing effect both on health care strategies and on patients. PMID:26251628

  6. Chemical Properties And Toxicity of Chromium(III) Nutritional Supplements

    SciTech Connect

    Levina, A.; Lay, P.A.

    2009-05-19

    The status of Cr(III) as an essential micronutrient for humans is currently under question. No functional Cr(III)-containing biomolecules have been definitively described as yet, and accumulated experience in the use of Cr(III) nutritional supplements (such as [Cr(pic){sub 3}], where pic = 2-pyridinecarboxylato) has shown no measurable benefits for nondiabetic people. Although the use of large doses of Cr(III) supplements may lead to improvements in glucose metabolism for type 2 diabetics, there is a growing concern over the possible genotoxicity of these compounds, particularly of [Cr(pic){sub 3}]. The current perspective discusses chemical transformations of Cr(III) nutritional supplements in biological media, with implications for both beneficial and toxic actions of Cr(III) complexes, which are likely to arise from the same biochemical mechanisms, dependent on concentrations of the reactive species. These species include: (1) partial hydrolysis products of Cr(III) nutritional supplements, which are capable of binding to biological macromolecules and altering their functions; and (2) highly reactive Cr(VI/V/IV) species and organic radicals, formed in reactions of Cr(III) with biological oxidants. Low concentrations of these species are likely to cause alterations in cell signaling (including enhancement of insulin signaling) through interactions with the active centers of regulatory enzymes in the cell membrane or in the cytoplasm, while higher concentrations are likely to produce genotoxic DNA lesions in the cell nucleus. These data suggest that the potential for genotoxic side-effects of Cr(III) complexes may outweigh their possible benefits as insulin enhancers, and that recommendations for their use as either nutritional supplements or antidiabetic drugs need to be reconsidered in light of these recent findings.

  7. Where are the Low-mass Population III Stars?

    NASA Astrophysics Data System (ADS)

    Ishiyama, Tomoaki; Sudo, Kae; Yokoi, Shingo; Hasegawa, Kenji; Tominaga, Nozomu; Susa, Hajime

    2016-07-01

    We study the number and the distribution of low-mass Population III (Pop III) stars in the Milky Way. In our numerical model, hierarchical formation of dark matter minihalos and Milky-Way-sized halos are followed by a high-resolution cosmological simulation. We model the Pop III formation in H2 cooling minihalos without metal under UV radiation of the Lyman–Werner bands. Assuming a Kroupa initial mass function (IMF) from 0.15 to 1.0 M ⊙ for low-mass Pop III stars, as a working hypothesis, we try to constrain the theoretical models in reverse by current and future observations. We find that the survivors tend to concentrate on the center of halo and subhalos. We also evaluate the observability of Pop III survivors in the Milky Way and dwarf galaxies, and constraints on the number of Pop III survivors per minihalo. The higher latitude fields require lower sample sizes because of the high number density of stars in the galactic disk, the required sample sizes are comparable in the high- and middle-latitude fields by photometrically selecting low-metallicity stars with optimized narrow-band filters, and the required number of dwarf galaxies to find one Pop III survivor is less than 10 at <100 kpc for the tip of red giant stars. Provided that available observations have not detected any survivors, the formation models of low-mass Pop III stars with more than 10 stars per minihalo are already excluded. Furthermore, we discuss the way to constrain the IMF of Pop III stars at a high mass range of ≳10 M ⊙.

  8. Dissimilatory Fe(III) and Mn(IV) reduction.

    PubMed Central

    Lovley, D R

    1991-01-01

    The oxidation of organic matter coupled to the reduction of Fe(III) or Mn(IV) is one of the most important biogeochemical reactions in aquatic sediments, soils, and groundwater. This process, which may have been the first globally significant mechanism for the oxidation of organic matter to carbon dioxide, plays an important role in the oxidation of natural and contaminant organic compounds in a variety of environments and contributes to other phenomena of widespread significance such as the release of metals and nutrients into water supplies, the magnetization of sediments, and the corrosion of metal. Until recently, much of the Fe(III) and Mn(IV) reduction in sedimentary environments was considered to be the result of nonenzymatic processes. However, microorganisms which can effectively couple the oxidation of organic compounds to the reduction of Fe(III) or Mn(IV) have recently been discovered. With Fe(III) or Mn(IV) as the sole electron acceptor, these organisms can completely oxidize fatty acids, hydrogen, or a variety of monoaromatic compounds. This metabolism provides energy to support growth. Sugars and amino acids can be completely oxidized by the cooperative activity of fermentative microorganisms and hydrogen- and fatty-acid-oxidizing Fe(III) and Mn(IV) reducers. This provides a microbial mechanism for the oxidation of the complex assemblage of sedimentary organic matter in Fe(III)- or Mn(IV)-reducing environments. The available evidence indicates that this enzymatic reduction of Fe(III) or Mn(IV) accounts for most of the oxidation of organic matter coupled to reduction of Fe(III) and Mn(IV) in sedimentary environments. Little is known about the diversity and ecology of the microorganisms responsible for Fe(III) and Mn(IV) reduction, and only preliminary studies have been conducted on the physiology and biochemistry of this process. PMID:1886521

  9. The Association of Human Apolipoprotein C-III Sialylation Proteoforms with Plasma Triglycerides

    PubMed Central

    Yassine, Hussein N.; Trenchevska, Olgica; Ramrakhiani, Ambika; Parekh, Aarushi; Koska, Juraj; Walker, Ryan W.; Billheimer, Dean; Reaven, Peter D.; Yen, Frances T.; Nelson, Randall W.; Goran, Michael I.; Nedelkov, Dobrin

    2015-01-01

    Introduction Apolipoprotein C-III (apoC-III) regulates triglyceride (TG) metabolism. In plasma, apoC-III exists in non-sialylated (apoC-III0a without glycosylation and apoC-III0b with glycosylation), monosialylated (apoC-III1) or disialylated (apoC-III2) proteoforms. Our aim was to clarify the relationship between apoC-III sialylation proteoforms with fasting plasma TG concentrations. Methods In 204 non-diabetic adolescent participants, the relative abundance of apoC-III plasma proteoforms was measured using mass spectrometric immunoassay. Results Compared with the healthy weight subgroup (n = 16), the ratios of apoC-III0a, apoC-III0b, and apoC-III1 to apoC-III2 were significantly greater in overweight (n = 33) and obese participants (n = 155). These ratios were positively correlated with BMI z-scores and negatively correlated with measures of insulin sensitivity (Si). The relationship of apoC-III1 / apoC-III2 with Si persisted after adjusting for BMI (p = 0.02). Fasting TG was correlated with the ratio of apoC-III0a / apoC-III2 (r = 0.47, p<0.001), apoC-III0b / apoC-III2 (r = 0.41, p<0.001), apoC-III1 / apoC-III2 (r = 0.43, p<0.001). By examining apoC-III concentrations, the association of apoC-III proteoforms with TG was driven by apoC-III0a (r = 0.57, p<0.001), apoC-III0b (r = 0.56. p<0.001) and apoC-III1 (r = 0.67, p<0.001), but not apoC-III2 (r = 0.006, p = 0.9) concentrations, indicating that apoC-III relationship with plasma TG differed in apoC-III2 compared with the other proteoforms. Conclusion We conclude that apoC-III0a, apoC-III0b, and apoC-III1, but not apoC- III2 appear to be under metabolic control and associate with fasting plasma TG. Measurement of apoC-III proteoforms can offer insights into the biology of TG metabolism in obesity. PMID:26633899

  10. Surprising Coordination for Plutonium in the First Plutonium (III) Borate

    SciTech Connect

    Wang, Shuao; Alekseev, Evgeny V.; Depmeier, Wulf; Albrecht-Schmitt, Thomas E.

    2011-02-22

    The first plutonium(III) borate, Pu2[B12O18(OH)4Br2(H2O)3]·0.5H2O, has been prepared by reacting plutonium(III) with molten boric acid under strictly anaerobic conditions. This compound contains a three-dimensional polyborate network with triangular holes that house the plutonium(III) sites. The plutonium sites in this compound are 9- and 10-coordinate and display atypical geometries.

  11. Inductrack III configuration--a maglev system for high loads

    DOEpatents

    Post, Richard F

    2013-11-12

    Inductrack III configurations are suited for use in transporting heavy freight loads. Inductrack III addresses a problem associated with the cantilevered track of the Inductrack II configuration. The use of a cantilevered track could present mechanical design problems in attempting to achieve a strong enough track system such that it would be capable of supporting very heavy loads. In Inductrack III, the levitating portion of the track can be supported uniformly from below, as the levitating Halbach array used on the moving vehicle is a single-sided one, thus does not require the cantilevered track as employed in Inductrack II.

  12. Inductrack III configuration--a maglev system for high loads

    DOEpatents

    Post, Richard F

    2015-03-24

    Inductrack III configurations are suited for use in transporting heavy freight loads. Inductrack III addresses a problem associated with the cantilevered track of the Inductrack II configuration. The use of a cantilevered track could present mechanical design problems in attempting to achieve a strong enough track system such that it would be capable of supporting very heavy loads. In Inductrack III, the levitating portion of the track can be supported uniformly from below, as the levitating Halbach array used on the moving vehicle is a single-sided one, thus does not require the cantilevered track as employed in Inductrack II.

  13. The Stratospheric Aerosol and Gas Experiment (SAGE III)

    NASA Technical Reports Server (NTRS)

    Thomason, Larry W.

    1998-01-01

    Three SAGE III instruments are being built by Ball Aerospace & Technologies Corporation in Boulder, Colorado (USA). SAGE III is a fourth generation instrument that incorporates robust elements of its predecessors [SAM II, SAGE, SAGE II] while incorporating new design elements. The first of these will be launched aboard a Russian Meteor/3M platform in May 1999. SAGE III will add measurements of O2-A band from which density and temperature profiles are retrieved. This feature should improve refraction and Rayleigh computations over earlier. Additionally, the linear array of detectors will permit on-orbit wavelength calibration from observations of the exo-atmospheric solar Fraunhofer spectrum.

  14. III-V Growth on Silicon Toward a Multijunction Cell

    SciTech Connect

    Geisz, J.; Olson, J.; McMahon, W.; Friedman, D.; Kibbler, A.; Kramer, C.; Young, M.; Duda, A.; Ward, S.; Ptak, A.; Kurtz, S.; Wanlass, M.; Ahrenkiel, P.; Jiang, C. S.; Moutinho, H.; Norman, A.; Jones, K.; Romero, M.; Reedy, B.

    2005-11-01

    A III-V on Si multijunction solar cell promises high efficiency at relatively low cost. The challenges to epitaxial growth of high-quality III-Vs on Si, though, are extensive. Lattice-matched (LM) dilute-nitride GaNPAs solar cells have been grown on Si, but their performance is limited by defects related to the nitrogen. Advances in the growth of lattice-mismatched (LMM) materials make more traditional III-Vs, such as GaInP and GaAsP, very attractive for use in multijunction solar cells on silicon.

  15. Luminescent cyclometallated iridium(III) complexes having acetylide ligands

    SciTech Connect

    Thompson, Mark E.; Bossi, Alberto; Djurovich, Peter Ivan

    2014-09-02

    The present invention relates to phosphorescent (triplet-emitting) organometallic materials. The phosphorescent materials of the present invention comprise Ir(III)cyclometallated alkynyl complexes for use as triplet light-emitting materials. The Ir(III)cyclometallated alkynyl complexes comprise at least one cyclometallating ligand and at least one alkynyl ligand bonded to the iridium. Also provided is an organic light emitting device comprising an anode, a cathode and an emissive layer between the anode and the cathode, wherein the emissive layer comprises a Ir(III)cyclometallated alkynyl complex as a triplet emitting material.

  16. No evidence for [O III] variability in Mrk 142

    NASA Astrophysics Data System (ADS)

    Barth, Aaron J.; Bentz, Misty C.

    2016-05-01

    Using archival data from the 2008 Lick AGN Monitoring Project, Zhang & Feng claimed to find evidence for flux variations in the narrow [O III] emission of the Seyfert 1 galaxy Mrk 142 over a two-month time span. If correct, this would imply a surprisingly compact size for the narrow-line region. We show that the claimed [O III] variations are merely the result of random errors in the overall flux calibration of the spectra. The data do not provide any support for the hypothesis that the [O III] flux was variable during the 2008 monitoring period.

  17. High efficiency III-nitride light-emitting diodes

    DOEpatents

    Crawford, Mary; Koleske, Daniel; Cho, Jaehee; Zhu, Di; Noemaun, Ahmed; Schubert, Martin F; Schubert, E. Fred

    2013-05-28

    Tailored doping of barrier layers enables balancing of the radiative recombination among the multiple-quantum-wells in III-Nitride light-emitting diodes. This tailored doping enables more symmetric carrier transport and uniform carrier distribution which help to reduce electron leakage and thus reduce the efficiency droop in high-power III-Nitride LEDs. Mitigation of the efficiency droop in III-Nitride LEDs may enable the pervasive market penetration of solid-state-lighting technologies in high-power lighting and illumination.

  18. Mexican-American male batterers on the MCMI-III.

    PubMed

    Sugihara, Y; Warner, J A

    1999-08-01

    This study examined personality characteristics of Mexican-American male batterers. 60 Mexican-American male batterers (M = 33.6 yr.) in the court system in South Texas took the MCMI-III and their MCMI-III scores were compared with the scores of a community sample of 45 Mexican-American individuals (M = 30.4 yr.). The batterers frequently scored higher than the nonbatterers on the Avoidant and Passive-Aggressive scales, while nonbatterers frequently scored higher on the Histrionic scale. The batterers scored significantly higher on 18 out of 24 MCMI-III scales, while nonbatterers scored significantly higher on two scales. PMID:10575982

  19. Bioactivity of pyridine-2-thiolato-1-oxide metal complexes: Bi(III), Fe(III) and Ga(III) complexes as potent anti-Mycobacterium tuberculosis prospective agents.

    PubMed

    Machado, Ignacio; Marino, Leonardo Biancolino; Demoro, Bruno; Echeverría, Gustavo A; Piro, Oscar E; Leite, Clarice Q F; Pavan, Fernando R; Gambino, Dinorah

    2014-11-24

    In the search for new therapeutic tools against tuberculosis and to further address the therapeutic potential of pyridine-2-thiol 1-oxide (Hmpo) metal complexes, two new octahedral [M(III)(mpo)3] complexes, with M = Ga or Bi, were synthesized and characterized in the solid state and in solution. Attempts to crystallize [Ga(III)(mpo)3] in CH2Cl2 led to single crystals of the reaction product [GaCl(mpo)2], where the gallium(III) ion is in a square basis pyramidal environment, trans-coordinated at the basis to two pyridine-2-thiolato 1-oxide anions acting as bidentate ligands through their oxygen and sulfur atoms. The biological activity of the new [M(III)(mpo)3] complexes together with that of the previously reported Fe(III) analogous compound and the pyridine-2-thiol 1-oxide sodium salt (Na mpo) was evaluated on Mycobacterium tuberculosis. The compounds showed excellent activity, both in the standard strain H37Rv ATCC 27294 (pan-susceptible) and in five clinical isolates that are resistant to the standard first-line anti-tuberculosis drugs isoniazid and rifampicin. These pyridine-2-thiol 1-oxide derivatives are promising compounds for the treatment of resistant tuberculosis. PMID:25261824

  20. The core helium flash revisited. III. From Population I to Population III stars

    NASA Astrophysics Data System (ADS)

    Mocák, M.; Campbell, S. W.; Müller, E.; Kifonidis, K.

    2010-09-01

    Context. Degenerate ignition of helium in low-mass stars at the end of the red giant branch phase leads to dynamic convection in their helium cores. One-dimensional (1D) stellar modeling of this intrinsically multi-dimensional dynamic event is likely to be inadequate. Previous hydrodynamic simulations imply that the single convection zone in the helium core of metal-rich Pop I stars grows during the flash on a dynamic timescale. This may lead to hydrogen injection into the core and to a double convection zone structure as known from one-dimensional core helium flash simulations of low-mass Pop III stars. Aims: We perform hydrodynamic simulations of the core helium flash in two and three dimensions to better constrain the nature of these events. To this end we study the hydrodynamics of convection within the helium cores of a 1.25 M_⊙ metal-rich Pop I star (Z = 0.02), and, for the first time, a 0.85 M_⊙ metal-free Pop III star (Z = 0) near the peak of the flash. These models possess single and double convection zones, respectively. Methods: We use 1D stellar models of the core helium flash computed with state-of-the-art stellar evolution codes as initial models for our multidimensional hydrodynamic study, and simulate the evolution of these models with the Riemann solver based hydrodynamics code Herakles, which integrates the Euler equations coupled with source terms corresponding to gravity and nuclear burning. Results: The hydrodynamic simulation of the Pop I model involving a single convection zone covers 27 h of stellar evolution, while the hydrodynamic simulations of a double convection zone, in the Pop III model, span 1.8 h of stellar life. We find differences between the predictions of mixing length theory and our hydrodynamic simulations. The simulation of the single convection zone in the Pop I model shows a strong growth of the size of the convection zone due to turbulent entrainment. We therefore predict that for the Pop I model a hydrogen injection

  1. Adsorption characteristics of U(VI) on Fe(III)Cr(III) (oxy)hydroxides synthesized at different temperatures.

    PubMed

    Ahn, Hyangsig; Jo, Ho Young; Lee, Young Jae; Kim, Geon-Young

    2016-07-01

    In this study, the adsorption behavior of U(VI) on (oxy)hydroxides synthesized at different temperatures (25 and 75 °C) was investigated. Four (oxy)hydroxides were synthesized by drying slurries of Fe(III) and Fe(III)Cr(III) (oxy)hydroxide in a vacuum desiccator (25 °C) or in an oven (75 °C). Batch adsorption tests were conducted using the (oxy)hydroxides thus synthesized and groundwater containing uranium ions. In general, the U(VI) removal fraction significantly increased with increasing pH from 3 to 5, remained constant with increasing pH from 5 to 9, and decreased at pH greater than 9, regardless of the type of (oxy)hydroxides and solid-to-liquid ratio. The effect of pH on the U(VI) removal fraction was more significant at a low solid-to-liquid ratio. The oven-dried Fe(III) (oxy)hydroxide exhibited a U(VI) removal fraction lower than that of the vacuum-dried one, whereas the oven-dried Fe(III)Cr(III) (oxy)hydroxide exhibited a U(VI) removal fraction higher than that exhibited by the vacuum-dried one. X-ray photoelectron spectroscopy (XPS) analysis results indicated that the difference in the U(VI) removal fraction is attributed to the dissolution and precipitation of the Fe(III) (oxy)hydroxide during oven drying and dehydration of the Fe(III)Cr(III) (oxy)hydroxide during oven drying. PMID:27060782

  2. Sparkle/AM1 Parameters for the Modeling of Samarium(III) and Promethium(III) Complexes.

    PubMed

    Freire, Ricardo O; da Costa, Nivan B; Rocha, Gerd B; Simas, Alfredo M

    2006-01-01

    The Sparkle/AM1 model is extended to samarium(III) and promethium(III) complexes. A set of 15 structures of high crystallographic quality (R factor < 0.05 Å), with ligands chosen to be representative of all samarium complexes in the Cambridge Crystallographic Database 2004, CSD, with nitrogen or oxygen directly bonded to the samarium ion, was used as a training set. In the validation procedure, we used a set of 42 other complexes, also of high crystallographic quality. The results show that this parametrization for the Sm(III) ion is similar in accuracy to the previous parametrizations for Eu(III), Gd(III), and Tb(III). On the other hand, promethium is an artificial radioactive element with no stable isotope. So far, there are no promethium complex crystallographic structures in CSD. To circumvent this, we confirmed our previous result that RHF/STO-3G/ECP, with the MWB effective core potential (ECP), appears to be the most efficient ab initio model chemistry in terms of coordination polyhedron crystallographic geometry predictions from isolated lanthanide complex ion calculations. We thus generated a set of 15 RHF/STO-3G/ECP promethium complex structures with ligands chosen to be representative of complexes available in the CSD for all other trivalent lanthanide cations, with nitrogen or oxygen directly bonded to the lanthanide ion. For the 42 samarium(III) complexes and 15 promethium(III) complexes considered, the Sparkle/AM1 unsigned mean error, for all interatomic distances between the Ln(III) ion and the ligand atoms of the first sphere of coordination, is 0.07 and 0.06 Å, respectively, a level of accuracy comparable to present day ab initio/ECP geometries, while being hundreds of times faster. PMID:26626380

  3. Structural Properties of the Cr(III)-Fe(III) (Oxy)hydroxide Compositional Series: Insights for a Nanomaterial "Solid Solution"

    SciTech Connect

    Michel, Y.; Michel, F; Zhang, L; Harrington, R; Parise, J; Reeder, R

    2010-01-01

    Chromium(III) (oxy)hydroxide and mixed Cr(III)-Fe(III) (oxy)hydroxides are environmentally important compounds for controlling chromium speciation and bioaccessibility in soils and aquatic systems and are also industrially important as precursors for materials and catalyst synthesis. However, direct characterization of the atomic arrangements of these materials is complicated because of their amorphous X-ray properties. This study involves synthesis of the complete Cr(III)-Fe(III) (oxy)hydroxide compositional series, and the use of complementary thermal, microscopic, spectroscopic, and scattering techniques for the evaluation of their structural properties. Thermal analysis results show that the Cr end member has a higher hydration state than the Fe end member, likely associated with the difference in water exchange rates in the first hydration spheres of Cr(III) and Fe(III). Three stages of weight loss are observed and are likely related to the loss of surface/structural water and hydroxyl groups. As compared to the Cr end member, the intermediate composition sample shows lower dehydration temperatures and a higher exothermic transition temperature. XANES analysis shows Cr(III) and Fe(III) to be the dominant oxidation states. XANES spectra also show progressive changes in the local structure around Cr and Fe atoms over the series. Pair distribution function (PDF) analysis of synchrotron X-ray total scattering data shows that the Fe end member is nanocrystalline ferrihydrite with an intermediate-range order and average coherent domain size of 27 {angstrom}. The Cr end member, with a coherent domain size of 10 {angstrom}, has only short-range order. The PDFs show progressive structural changes across the compositional series. High-resolution transmission electron microscopy (HRTEM) results also show the loss of structural order with increasing Cr content. These observations provide strong structural evidence of chemical substitution and progressive structural

  4. Extended Deterrence, Nuclear Proliferation, and START III

    SciTech Connect

    Speed, R.D.

    2000-06-20

    Early in the Cold War, the United States adopted a policy of ''extended nuclear deterrence'' to protect its allies by threatening a nuclear strike against any state that attacks these allies. This threat can (in principle) be used to try to deter an enemy attack using conventional weapons or one using nuclear, chemical, or biological weapons. The credibility of a nuclear threat has long been subject to debate and is dependent on many complex geopolitical factors, not the least of which is the military capabilities of the opposing sides. The ending of the Cold War has led to a significant decrease in the number of strategic nuclear weapons deployed by the United States and Russia. START II, which was recently ratified by the Russian Duma, will (if implemented) reduce the number deployed strategic nuclear weapons on each side to 3500, compared to a level of over 11,000 at the end of the Cold War in 1991. The tentative limit established by Presidents Clinton and Yeltsin for START III would reduce the strategic force level to 2000-2500. However, the Russians (along with a number of arms control advocates) now argue that the level should be reduced even further--to 1500 warheads or less. The conventional view is that ''deep cuts'' in nuclear weapons are necessary to discourage nuclear proliferation. Thus, as part of the bargain to get the non-nuclear states to agree to the renewal of the Nuclear Non-Proliferation Treaty, the United States pledged to work towards greater reductions in strategic forces. Without movement in the direction of deep cuts, it is thought by many analysts that some countries may decide to build their own nuclear weapons. Indeed, this was part of the rationale India used to justify its own nuclear weapons program. However, there is also some concern that deep cuts (to 1500 or lower) in the U.S. strategic nuclear arsenal could have the opposite effect. The fear is that such cuts might undermine extended deterrence and cause a crisis in confidence

  5. 4. Historic American Buildings Survey George Harkness III, Photographer, April ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey George Harkness III, Photographer, April 10, 1934 MEASURING THE WEST ELEVATION (REAR) - Jean Baptiste Valle House, 99 South Main Street (Northwest corner of Main & Market Streets), Sainte Genevieve, Ste. Genevieve County, MO

  6. Synthesis, structure and luminescence studies of Eu(III), Tb(III), Sm(III), Dy(III) cationic complexes with acetylacetone and bis(5-(pyridine-2-yl)-1,2,4-triazol-3-yl)propane☆

    PubMed Central

    Gusev, Alexey N.; Hasegawa, Miki; Shimizu, Tomohito; Fukawa, Tomonori; Sakurai, Shoya; Nishchymenko, Galyna A.; Shul’gin, Victor F.; Meshkova, Svetlana B.; Linert, Wolfgang

    2013-01-01

    Studies concerning synthesis, structure and luminescence of eight-coordinate Eu, Tb, Sm and Dy complexes of the type [Ln(acac)2(L)]Cl (Hacac = pentanedione-2,4 and L = bis(5-(pyridine-2-yl)-1,2,4-triazol-3-yl)propane) are reported in detail. The obtained complexes were investigated by various means including elemental- and thermogravimetric analysis, IR- and electron transition spectroscopy. The structure of the Tb complex was determined by single-crystal X-ray crystallography: Tb is eight-coordinate, and L acting only as a tetradentate chelate together with two bidentate acac ligands. Photophysical studies of the complexes were carried out. The Tb(III) and Eu(III) complexes show strong emissions both in solid state and solution. The intensity of the luminescence of Dy(III) and Sm(III) are relatively weak. The factors determining the intensity of the photoluminescence are discussed. PMID:24068839

  7. Assessing PTSD with the Millon Clinical Multiaxial Inventory-III.

    PubMed

    Craig, R J; Olson, R

    1997-12-01

    We studied the utility of the Millon Clinical Multiaxial Inventory-III (MCMI-III) in assessing substance-abusing (n = 228), combat-related PTSD patients (n = 32). The MCMI-III produced a code type (16A) that was quite different from MCMI-I and MCMI-II code types (8A2) among similar populations. The PTSD Scale (R) successfully differentiated between a PTSD and non-PTSD substance-abusing group using mean Base Rate scores, was the best predictor of PTSD in a multiple regression equation, and the scale's sensitivity and specificity in detecting and/or ruling out the disorder was above that provided by chance alone and higher than the values reported in the test manual for that scale. The MCMI-III may be used as a broad band screening instrument for PTSD, at least among patients with combat-related stress. PMID:9403398

  8. Zone III flexor tendon injuries - A proposed modification to rehabilitation.

    PubMed

    Chinchalkar, Shrikant J; Pipicelli, Joey G; Agur, Anne; Athwal, George S

    2015-01-01

    In this manuscript, these authors have utilized years of clinical experience to suggest rehabilitation modifications for Zone III flexor tendon injuries. - VictoriaPriganc, PhD, OTR, CHT, CLT, Practice Forum Editor. PMID:26089286

  9. III-V High-Efficiency Multijunction Photovoltaics (Fact Sheet)

    SciTech Connect

    Not Available

    2011-06-01

    Capabilities fact sheet that includes scope, core competencies and capabilities, and contact/web information for III-V High-Efficiency Multijunction Photovoltaics at the National Center for Photovoltaics.

  10. 5. Historic American Buildings Survey Photographer, Robert Fulton III, June ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey Photographer, Robert Fulton III, June 1967 INTERIOR, LOOKING WEST - Third Congregational Society, Church of the Redeemer, 292 Orange Street, New Haven, New Haven County, CT

  11. 5. Historic American Buildings Survey Robert Fulton III, Photographer June ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey Robert Fulton III, Photographer June 1967 FRONT FACADE FACING NEW HAVEN GREEN - First Church of Christ, Congregational, Temple Street, New Haven, New Haven County, CT

  12. 3. Historic American Buildings Survey Robert Fulton III, Photographer June ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey Robert Fulton III, Photographer June 1967 MAIN BANKING ROOM ENTRANCE, FACING SOUTH - Townsend City Savings Bank, 793 Chapel Street, New Haven, New Haven County, CT

  13. 3. Historic American Buildings Survey Robert Fulton III, Photographer, June ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey Robert Fulton III, Photographer, June 1967 REAR OF CITY HALL - New Haven City Hall & Courthouse, Church Street, between Court & Elm Streets, New Haven, New Haven County, CT

  14. 3. Historic American Buildings Survey Robert Fulton III, Photographer, June ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey Robert Fulton III, Photographer, June 1964 NORTHWEST (REAR) FACADE, FACING HIGH STREET - Yale University, Dwight Hall, 69 High Street, New Haven, New Haven County, CT

  15. 6. Historic American Buildings Survey Robert Fulton III, Photographer, June ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Historic American Buildings Survey Robert Fulton III, Photographer, June 1967 CHOIR LOFT, LOOKING EAST - Third Congregational Society, Church of the Redeemer, 292 Orange Street, New Haven, New Haven County, CT

  16. 2. Historic American Buildings Survey Photographer, Robert Fulton III, June ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey Photographer, Robert Fulton III, June 1967 EXTERIOR, FACING WALL STREET - Third Congregational Society, Church of the Redeemer, 292 Orange Street, New Haven, New Haven County, CT

  17. 40 CFR 300.220 - Related Title III issues.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... are found in 40 CFR part 355. ... 40 Protection of Environment 27 2010-07-01 2010-07-01 false Related Title III issues. 300.220 Section 300.220 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND,...

  18. 40 CFR 300.220 - Related Title III issues.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... are found in 40 CFR part 355. ... 40 Protection of Environment 28 2014-07-01 2014-07-01 false Related Title III issues. 300.220 Section 300.220 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND,...

  19. 40 CFR 300.220 - Related Title III issues.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... are found in 40 CFR part 355. ... 40 Protection of Environment 29 2013-07-01 2013-07-01 false Related Title III issues. 300.220 Section 300.220 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND,...

  20. 40 CFR 300.220 - Related Title III issues.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... are found in 40 CFR part 355. ... 40 Protection of Environment 29 2012-07-01 2012-07-01 false Related Title III issues. 300.220 Section 300.220 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND,...

  1. 40 CFR 300.220 - Related Title III issues.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... are found in 40 CFR part 355. ... 40 Protection of Environment 28 2011-07-01 2011-07-01 false Related Title III issues. 300.220 Section 300.220 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND,...

  2. Genetics Home Reference: Pol III-related leukodystrophy

    MedlinePlus

    ... Pol III-related leukodystrophy often have abnormalities in eye movement, such as progressive vertical gaze palsy, which is restricted up-and-down eye movement that worsens over time. Nearsightedness is common in ...

  3. Components of Adult Class III Malocclusion in an Iranian Population

    PubMed Central

    Koodaryan, Roodabeh; Rafighi, Ali; Hafezeqoran, Ali

    2009-01-01

    Background and aims Class III malocclusions are considered complex and difficult orthodontic problems to diagnose and treat. The purpose of this study was to investigate the morphologic characteristics of the craniofacial complex of adults with Class III malocclusion in an Iranian population. Materials and methods Lateral cephalometric radiographs of 50 patients with Class III malocclusion (20 males and 30 females; age range of 18-27 years) were selected on the basis of molar relationship, concave profile and an overjet of ≤ 0 mm. The standard values of 19 soft tissue measurements were determined using McNamara, Burstone and Viazis methods. Results Adults with Class III malocclusion exhibited distinct craniofacial morphologic characteristics manifested by a com-bination of retrusion of maxilla and protrusion of mandible. Conclusion The most prevalent component was mandibular prognathism, normal maxilla and LAFH on the basis of the component analysis. PMID:23230476

  4. REVISITING THE FIRST GALAXIES: THE EPOCH OF POPULATION III STARS

    SciTech Connect

    Muratov, Alexander L.; Gnedin, Oleg Y.; Zemp, Marcel; Gnedin, Nickolay Y.

    2013-08-10

    We investigate the transition from primordial Population III (Pop III) star formation to normal Pop II star formation in the first galaxies using new cosmological hydrodynamic simulations. We find that while the first stars seed their host galaxies with metals, they cannot sustain significant outflows to enrich the intergalactic medium, even assuming a top-heavy initial mass function. This means that Pop III star formation could potentially continue until z Almost-Equal-To 6 in different unenriched regions of the universe, before being ultimately shut off by cosmic reionization. Within an individual galaxy, the metal production and stellar feedback from Pop II stars overtake Pop III stars in 20-200 Myr, depending on galaxy mass.

  5. CVD diamond screens for photon beam imaging at PETRA III

    NASA Astrophysics Data System (ADS)

    Degenhardt, M.; Aprigliano, G.; Schulte-Schrepping, H.; Hahn, U.; Grabosch, H.-J.; Wörner, E.

    2013-03-01

    PETRA III, the most brilliant storage-ring-based synchrotron radiation source in the world, started its operation in 2009. It features 14 undulator beamlines and will be extended by further 10 beamlines in the PETRA III extension project. During the startup phase of the 14 PETRA III beamlines, fluorescence monitors based on CVD diamond screens have proven to be a very powerful tool for the monitoring of the attenuated undulator beams and for the commissioning of the optical components, e.g. slit systems and monochromators. They served as the essential instrument for the initial setup of the positron beam orbit to align the undulator photon beam along the beamline. The application of CVD diamond screens for the beam imaging at PETRA III beamlines is presented. Images taken during the beam adjustment and the beamline commissioning are shown.

  6. Decametric and hectometric Solar Type III bursts at Saturn's orbit

    NASA Astrophysics Data System (ADS)

    Boudjada, Mohammed Y.; Sawas, Sami; Galopeau, Patrick H. M.; Maksimovic, Milan

    2015-04-01

    We report on solar radio bursts observed by RPWS experiment onboard Cassini spacecraft. We consider Type III solar bursts observed in the frequency range from 1 MHz to 16 MHz. Those bursts are probably generated in the solar corona and the interplanetary medium. We show that the Type III burst occurrence is depending on the solar activity. We attempt to localize the regions where the Type III burst is probably emitted. We consider that the electrons at the origin of the Solar Type III bursts follow the interplanetary magnetic field. The trajectory is an Archimedean spiral contained in the ecliptic plane. We discuss our results taking into consideration on the one hand the spacecraft positions with regards to the source location, and on the other hand the temporal and spectral radio beam variation when combining Cassini and Wind observations.

  7. 4. Historic American Buildings Survey George Harkness III, Photographer, April ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey George Harkness III, Photographer, April 10, 1934 VIEW OF DOME AND LANTERN FROM NORTH - Old St. Louis Courthouse, Fourth to Broadway, Market to Chestnut Streets, Saint Louis, Independent City, MO

  8. 3. Historic American Buildings Survey George Harkness III, Photographer, April ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey George Harkness III, Photographer, April 10, 1934 VIEW OF DOME AND DRUM FROM NORTH - Old St. Louis Courthouse, Fourth to Broadway, Market to Chestnut Streets, Saint Louis, Independent City, MO

  9. An oxygen-sensitive luminescent Dy(iii) complex.

    PubMed

    Nakai, Hidetaka; Seo, Juncheol; Kitagawa, Kazuhiro; Goto, Takahiro; Matsumoto, Takahiro; Ogo, Seiji

    2016-06-21

    This paper presents the first dysprosium(iii) complex, [{((MeMe)ArO)3tacn}Dy(III)(THF)] (1(Dy)), that shows oxygen-sensitive luminescence. The synthesis, structure and oxygen-sensitive luminescence properties of 1(Dy) are reported (Φ = 0.050 and τ = 17.7 μs under N2, Φ = 0.011 and τ = 4.1 μs under O2 and KSV = 305 M(-1) in THF; KSV = 0.0077%(-1) in polystyrene film). The oxygen sensitive mechanism of 1(Dy) is discussed based on the photophysical properties of the corresponding gadolinium(iii) complex, [{((MeMe)ArO)3tacn}Gd(III)(THF)]. PMID:27191980

  10. Studies of Iron(III) Porphyrinates Containing Silylthiolate Ligands

    PubMed Central

    Meininger, Daniel J.; Caranto, Jonathan D.; Arman, Hadi D.

    2014-01-01

    The chemistry of several iron(III) porphyrinates containing silylthiolate ligands is described. The complexes are prepared by protonolysis reactions of silanethiols with the iron(III) precursors, [Fe(OMe)(TPP)] and [Fe(OH)(H2O)(TMP)] (TPP = dianion of meso-tetraphenylporphine; TMP = dianion of meso-tetramesitylporphine). Each of the compounds has been fully characterized in solution and the solid state. The stability of the silylthiolate complexes versus other iron(III) porphyrinate complexes containing sulfur-based ligands allows for an examination of their reactivity with several biologically relevant small molecules including H2S, NO, and 1-methylimidazole. Electrochemically, the silylthiolate complexes display a quasi-reversible one-electron oxidation event at potentials higher than that observed for an analogous arylthiolate complex. The behavior of these complexes versus other sulfur-ligated iron(III) porphyrinates is discussed. PMID:24138018

  11. Substructure Main Bridge, River Piers I, II, III & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Substructure - Main Bridge, River Piers I, II, III & IV - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  12. Development of a C3-symmetric benzohydroxamate tripod: Trimetallic complexation with Fe(III), Cr(III) and Al(III)

    NASA Astrophysics Data System (ADS)

    Baral, Minati; Gupta, Amit; Kanungo, B. K.

    2016-06-01

    The design, synthesis and physicochemical characterization of a C3-symmetry Benzene-1,3,5-tricarbonylhydroxamate tripod, noted here as BTHA, are described. The chelator was built from a benzene as an anchor, symmetrically extended by three hydroxamate as ligating moieties, each bearing O, O donor sites. A combination of absorption spectrophotometry, potentiometry and theoretical investigations are used to explore the complexation behavior of the ligand with some trivalent metal ions: Fe(III), Cr(III), and Al(III). Three protonation constants were calculated for the ligand in a pH range of 2-11 in a highly aqueous medium (9:1 H2O: DMSO). A high rigidity in the molecular structure restricts the formation of 1:1 (M/L) metal encapsulation but shows a high binding efficiency for a 3:1 metal ligand stoichiometry giving formation constant (in β unit) 28.73, 26.13 and 19.69 for [M3L]; Mdbnd Fe(III), Al(III) and Cr(III) respectively, and may be considered as an efficient Fe-carrier. The spectrophotometric study reveals of interesting electronic transitions occurred during the complexation. BTHA exhibits a peak at 238 nm in acidic pH and with the increase of pH, a new peak appeared at 270 nm. A substantial shifting in both of the peaks in presence of the metal ions implicates a s coordination between ligand and metal ions. Moreover, complexation of BTHA with iron shows three distinct colors, violet, reddish orange and yellow in different pH, enables the ligand to be considered for the use as colorimetric sensor.

  13. Development of a C3-symmetric benzohydroxamate tripod: Trimetallic complexation with Fe(III), Cr(III) and Al(III).

    PubMed

    Baral, Minati; Gupta, Amit; Kanungo, B K

    2016-06-01

    The design, synthesis and physicochemical characterization of a C3-symmetry Benzene-1,3,5-tricarbonylhydroxamate tripod, noted here as BTHA, are described. The chelator was built from a benzene as an anchor, symmetrically extended by three hydroxamate as ligating moieties, each bearing O, O donor sites. A combination of absorption spectrophotometry, potentiometry and theoretical investigations are used to explore the complexation behavior of the ligand with some trivalent metal ions: Fe(III), Cr(III), and Al(III). Three protonation constants were calculated for the ligand in a pH range of 2-11 in a highly aqueous medium (9:1 H2O: DMSO). A high rigidity in the molecular structure restricts the formation of 1:1 (M/L) metal encapsulation but shows a high binding efficiency for a 3:1 metal ligand stoichiometry giving formation constant (in β unit) 28.73, 26.13 and 19.69 for [M3L]; MFe(III), Al(III) and Cr(III) respectively, and may be considered as an efficient Fe-carrier. The spectrophotometric study reveals of interesting electronic transitions occurred during the complexation. BTHA exhibits a peak at 238nm in acidic pH and with the increase of pH, a new peak appeared at 270nm. A substantial shifting in both of the peaks in presence of the metal ions implicates a s coordination between ligand and metal ions. Moreover, complexation of BTHA with iron shows three distinct colors, violet, reddish orange and yellow in different pH, enables the ligand to be considered for the use as colorimetric sensor. PMID:26970809

  14. Suppressors of superoxide production from mitochondrial complex III

    PubMed Central

    Orr, Adam L.; Vargas, Leonardo; Turk, Carolina N.; Baaten, Janine E.; Matzen, Jason T.; Dardov, Victoria J.; Attle, Stephen J.; Li, Jing; Quackenbush, Douglas C.; Goncalves, Renata L. S.; Perevoshchikova, Irina V.; Petrassi, H. Michael; Meeusen, Shelly L.; Ainscow, Edward K.; Brand, Martin D.

    2015-01-01

    Mitochondrial electron transport drives ATP synthesis but also generates reactive oxygen species (ROS), which are both cellular signals and damaging oxidants. Superoxide production by respiratory complex III is implicated in diverse signaling events and pathologies but its role remains controversial. Using high-throughput screening we identified compounds that selectively eliminate superoxide production by complex III without altering oxidative phosphorylation; they modulate retrograde signaling including cellular responses to hypoxic and oxidative stress. PMID:26368590

  15. Suppressors of superoxide production from mitochondrial complex III.

    PubMed

    Orr, Adam L; Vargas, Leonardo; Turk, Carolina N; Baaten, Janine E; Matzen, Jason T; Dardov, Victoria J; Attle, Stephen J; Li, Jing; Quackenbush, Douglas C; Goncalves, Renata L S; Perevoshchikova, Irina V; Petrassi, H Michael; Meeusen, Shelly L; Ainscow, Edward K; Brand, Martin D

    2015-11-01

    Mitochondrial electron transport drives ATP synthesis but also generates reactive oxygen species, which are both cellular signals and damaging oxidants. Superoxide production by respiratory complex III is implicated in diverse signaling events and pathologies, but its role remains controversial. Using high-throughput screening, we identified compounds that selectively eliminate superoxide production by complex III without altering oxidative phosphorylation; they modulate retrograde signaling including cellular responses to hypoxic and oxidative stress. PMID:26368590

  16. Progress Towards III-V Photovoltaics on Flexible Substrates

    NASA Technical Reports Server (NTRS)

    McNatt, Jeremiah S.; Pal, AnnaMaria T.; Clark, Eric B.; Sayir, Ali; Raffaelle, Ryne P.; Bailey, Christopher G.; Hubbard, Seth M.; Maurer, William F.; Fritzemeier, Les

    2008-01-01

    Presented here is the recent progress of the NASA Glenn Research Center OMVPE group's efforts in the development of high efficiency thin-film polycrystalline III-V photovoltaics on optimum substrates. By using bulk polycrystalline germanium (Ge) films, devices of high efficiency and low mass will be developed and incorporated onto low-cost flexible substrates. Our progress towards the integration of high efficiency polycrystalline III-V devices and recrystallized Ge films on thin metal foils is discussed.

  17. Silver(II) Oxide or Silver(I,III) Oxide?

    ERIC Educational Resources Information Center

    Tudela, David

    2008-01-01

    The often called silver peroxide and silver(II) oxide, AgO or Ag[subscript 2]O[subscript 2], is actually a mixed oxidation state silver(I,III) oxide. A thermochemical cycle, with lattice energies calculated within the "volume-based" thermodynamic approach, explain why the silver(I,III) oxide is more stable than the hypothetical silver(II) oxide.…

  18. PLCO Ovarian Phase III Validation Study — EDRN Public Portal

    Cancer.gov

    Our preliminary data indicate that the performance of CA 125 as a screening test for ovarian cancer can be improved upon by additional biomarkers. With completion of one additional validation step, we will be ready to test the performance of a consensus marker panel in a phase III validation study. Given the original aims of the PLCO trial, we believe that the PLCO represents an ideal longitudinal cohort offering specimens for phase III validation of ovarian cancer biomarkers.

  19. 43. Photograph of a line drawing. 'PART III, SECTION 1, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    43. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS H-1 TO H-10 INCL., GRINDING, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

  20. 38. Photograph of a line drawing. 'PART III, SECTION 1, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS G-1 TO G-10 INCL., PURIFICATION, MANUFACTURING AREA, PLAN 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

  1. Different Interaction Mechanisms of Eu(III) and (243)Am(III) with Carbon Nanotubes Studied by Batch, Spectroscopy Technique and Theoretical Calculation.

    PubMed

    Wang, Xiangxue; Yang, Shubin; Shi, Weiqun; Li, Jiaxing; Hayat, Tasawar; Wang, Xiangke

    2015-10-01

    Herein the sorption of Eu(III) and (243)Am(III) on multiwalled carbon nanotubes (CNTs) are studied, and the results show that Eu(III) and (243)Am(III) could form strong inner-sphere surface complexes on CNT surfaces. However, the sorption of Eu(III) on CNTs is stronger than that of (243)Am(III) on CNTs, suggesting the difference in the interaction mechanisms or properties of Eu(III) and (243)Am(III) with CNTs, which is quite different from the results of Eu(III) and (243)Am(III) interaction on natural clay minerals and oxides. On the basis of the results of density functional theory calculations, the binding energies of Eu(III) on CNTs are much higher than those of (243)Am(III) on CNTs, indicating that Eu(III) could form stronger complexes with the oxygen-containing functional groups of CNTs than (243)Am(III), which is in good agreement with the experimental results of higher sorption capacity of CNTs for Eu(III). The oxygen-containing functional groups contribute significantly to the uptake of Eu(III) and (243)Am(III), and the binding affinity increases in the order of ≡S-OH < ≡S-COOH < ≡S-COO(-). This paper highlights the interaction mechanism of Eu(III) and (243)Am(III) with different oxygen-containing functional groups of CNTs, which plays an important role for the potential application of CNTs in the preconcentration, removal, and separation of trivalent lanthanides and actinides in environmental pollution cleanup. PMID:26371690

  2. Mode III fatigue crack propagation in low alloy steel

    NASA Astrophysics Data System (ADS)

    Ritchie, R. O.; McClintock, F. A.; Nayeb-Hashemi, H.; Ritter, M. A.

    1982-01-01

    To provide a basis for estimating fatigue life in large rotating generator shafts subjected to transient oscillations, a study is made of fatigue crack propagation in Mode III (anti-plane shear) in torsionally-loaded spheroidized AISI4340 steel, and results compared to analogous behavior in Mode I. Torsional S/N curves, determined on smooth bars containing surface defects, showed results surprisingly close to expected unnotched Mode I data, with lifetime increasing from 104 cycles at nominal yield to 106 cycles at half yield. Fatigue crack growth rates in Mode III, measured on circumferentially-notched samples, were found to be slower than in Mode I, although still power-law related to the alternating stress intensity (△K III) for small-scale yielding. Mode III growth rates were only a small fraction (0.002 to 0.0005) of cyclic crack tip displacements (△CTD III) per cycle, in contrast to Mode I where the fraction was much larger (0.1 to 0.01). A micromechanical model for Mode III growth is proposed, where crack advance is considered to take place by a Mode II coalescence of cracks, initiated at inclusions ahead of the main crack front. This mechanism is consistent with the crack increment being a small fraction of △CTDIII per cycle.

  3. FORMATION CRITERIA AND THE MASS OF SECONDARY POPULATION III STARS

    SciTech Connect

    Susa, Hajime; Umemura, Masayuki; Hasegawa, Kenji E-mail: umemura@ccs.tsukuba.ac.jp

    2009-09-01

    We explore the formation of secondary Population III (Pop III) stars under radiation hydrodynamic (RHD) feedback by a preformed massive star. To properly treat RHD feedback, we perform three-dimensional RHD simulations incorporating the radiative transfer of ionizing photons as well as H{sub 2} dissociating photons from a preformed star. A collapsing gas cloud is settled at a given distance from a 120 M{sub sun} Pop III star, and the evolution of the cloud is pursued including RHD feedback. We derive the threshold density depending on the distance, above which the cloud can keep collapsing owing to the shielding of H{sub 2} dissociating radiation. We find that an H{sub 2} shell formed ahead of an ionizing front works effectively to shield the H{sub 2} dissociating radiation, leading to the positive feedback for the secondary Pop III star formation. Also, near the threshold density, the envelope of gas cloud is stripped significantly by a shock associated with an ionizing front. By comparing the mass accretion timescale with the Kelvin-Helmholtz timescale, we estimate the mass of secondary Pop III stars. It turns out that the stripping by a shock can reduce the mass of secondary Pop III stars down to {approx}20 M{sub sun}.

  4. Effect of Population III Multiplicity on Dark Star Formation

    NASA Technical Reports Server (NTRS)

    Stacy, Athena; Pawlik, Andreas H.; Bromm, Volker; Loeb, Abraham

    2012-01-01

    We numerically study the mutual interaction between dark matter (DM) and Population III (Pop III) stellar systems in order to explore the possibility of Pop III dark stars within this physical scenario. We perform a cosmological simulation, initialized at z approx. 100, which follows the evolution of gas and DM. We analyze the formation of the first mini halo at z approx. 20 and the subsequent collapse of the gas to densities of 10(exp 12)/cu cm. We then use this simulation to initialize a set of smaller-scale 'cut-out' simulations in which we further refine the DM to have spatial resolution similar to that of the gas. We test multiple DM density profiles, and we employ the sink particle method to represent the accreting star-forming region. We find that, for a range of DM configurations, the motion of the Pop III star-disk system serves to separate the positions of the protostars with respect to the DM density peak, such that there is insufficient DM to influence the formation and evolution of the protostars for more than approx. 5000 years. In addition, the star-disk system causes gravitational scattering of the central DM to lower densities, further decreasing the influence of DM over time. Any DM-powered phase of Pop III stars will thus be very short-lived for the typical multiple system, and DM will not serve to significantly prolong the life of Pop III stars.

  5. Mechanism of Ribonuclease III Catalytic Regulation by Serine Phosphorylation

    PubMed Central

    Gone, Swapna; Alfonso-Prieto, Mercedes; Paudyal, Samridhdi; Nicholson, Allen W.

    2016-01-01

    Ribonuclease III (RNase III) is a conserved, gene-regulatory bacterial endonuclease that cleaves double-helical structures in diverse coding and noncoding RNAs. RNase III is subject to multiple levels of control, reflective of its global regulatory functions. Escherichia coli (Ec) RNase III catalytic activity is known to increase during bacteriophage T7 infection, reflecting the expression of the phage-encoded protein kinase, T7PK. However, the mechanism of catalytic enhancement is unknown. This study shows that Ec-RNase III is phosphorylated on serine in vitro by purified T7PK, and identifies the targets as Ser33 and Ser34 in the N-terminal catalytic domain. Kinetic experiments reveal a 5-fold increase in kcat and a 1.4-fold decrease in Km following phosphorylation, providing a 7.4–fold increase in catalytic efficiency. Phosphorylation does not change the rate of substrate cleavage under single-turnover conditions, indicating that phosphorylation enhances product release, which also is the rate-limiting step in the steady-state. Molecular dynamics simulations provide a mechanism for facilitated product release, in which the Ser33 phosphomonoester forms a salt bridge with the Arg95 guanidinium group, thereby weakening RNase III engagement of product. The simulations also show why glutamic acid substitution at either serine does not confer enhancement, thus underscoring the specific requirement for a phosphomonoester. PMID:27150669

  6. Uranium (III) precipitation in molten chloride by wet argon sparging

    NASA Astrophysics Data System (ADS)

    Vigier, Jean-François; Laplace, Annabelle; Renard, Catherine; Miguirditchian, Manuel; Abraham, Francis

    2016-06-01

    In the context of pyrochemical processes for nuclear fuel treatment, the precipitation of uranium (III) in molten salt LiCl-CaCl2 (30-70 mol%) at 705 °C is studied. First, this molten chloride is characterized with the determination of the water dissociation constant. With a value of 10-4.0, the salt has oxoacid properties. Then, the uranium (III) precipitation using wet argon sparging is studied. The salt is prepared using UCl3 precursor. At the end of the precipitation, the salt is totally free of solubilized uranium. The main part is converted into UO2 powder but some uranium is lost during the process due to the volatility of uranium chloride. The main impurity of the resulting powder is calcium. The consequences of oxidative and reductive conditions on precipitation are studied. Finally, coprecipitation of uranium (III) and neodymium (III) is studied, showing a higher sensitivity of uranium (III) than neodymium (III) to precipitation.

  7. Assessment of hoist failure rate for Payload Transporter III

    SciTech Connect

    Demmie, P.N.

    1994-02-01

    Assessment of the hoist failure rate for the Payload Transporter Type III (PT-III) hoist was completed as one of the ground transportation tasks for the Minuteman II (MMIII) Weapon System Safety Assessment. The failures of concern are failures that lead to dropping a reentry system (RS) during hoist operations in a silo or the assembly, storage, and inspection building for a MMIII wing. After providing a brief description of the PT-III hoist system, the author summarizes his search for historical data from industry and the military services for failures of electric hoist systems. Since such information was not found, the strategy for assessing a failure rate was to consider failure mechanisms which lead to load-drop accidents, estimate their rates, and sum the rates for the PT-III hoist failure rate. The author discusses failure mechanisms and describes his assessment of a chain failure rate that is based on data from destructive testing of a chain of the type used for the PT-III hoist and projected usage rates for hoist operations involving the RS. The main result provides upper bounds for chain failure rates that are based on these data. No test data were found to estimate failure rates due to mechanisms other than chain failure. The author did not attempt to quantify the effects of human factors on the PT-III hoist failure rate.

  8. Involvement of RNA Polymerase III in Immune Responses

    PubMed Central

    White, Robert J.

    2015-01-01

    Inflammation in the tumor microenvironment has many tumor-promoting effects. In particular, tumor-associated macrophages (TAMs) produce many cytokines which can support tumor growth by promoting survival of malignant cells, angiogenesis, and metastasis. Enhanced cytokine production by TAMs is tightly coupled with protein synthesis. In turn, translation of proteins depends on tRNAs, short abundant transcripts that are made by RNA polymerase III (Pol III). Here, we connect these facts by showing that stimulation of mouse macrophages with lipopolysaccharides (LPS) from the bacterial cell wall causes transcriptional upregulation of tRNA genes. The transcription factor NF-κB is a key transcription factor mediating inflammatory signals, and we report that LPS treatment causes an increased association of the NF-κB subunit p65 with tRNA genes. In addition, we show that p65 can directly associate with the Pol III transcription factor TFIIIB and that overexpression of p65 induces Pol III-dependent transcription. As a consequence of these effects, we show that inhibition of Pol III activity in macrophages restrains cytokine secretion and suppresses phagocytosis, two key functional characteristics of these cells. These findings therefore identify a radical new function for Pol III in the regulation of macrophage function which may be important for the immune responses associated with both normal and malignant cells. PMID:25776554

  9. Empirical evaluation of the MCMI-III personality disorder scales.

    PubMed

    Rossi, Gina; Hauben, Claudia; Van den Brande, Iris; Sloore, Hedwig

    2003-04-01

    The MCMI-III personality disorder scales were empirically validated with a sample of 870 clinical patients and inmates. Prevalence rates of personality disorders were in general lower on the MCMI-III than clinical ratings, but trait prevalence was generally higher; thus a base rate of 75 on the MCMI-III could be a guideline in the screening of trait prevalence. However, the sensitivity of some MCMI-III scales was very low. Moreover, the correlations of most personality disorder scales of the MCMI-III were significant and positive with corresponding measures on clinical ratings and MMPI-2 personality disorder scales, but these were, in general, not significantly higher than some other correlations. As a consequence the discriminant validity seems to be questionable. The MCMI-III alone cannot be used as a diagnostic inventory, but the test could be useful as a screening device as a part of a multimethod approach that allows aggregation over measures in making diagnostic decisions. PMID:12785653

  10. Revisiting The First Galaxies: The Epoch of Population III Stars

    NASA Astrophysics Data System (ADS)

    Muratov, Alexander; Gnedin, O. Y.; Gnedin, N. Y.; Zemp, M. K.

    2013-01-01

    We study the formation of the first galaxies using new hydrodynamic cosmological simulations with the ART code. Our simulations feature a recently developed model for dust-based formation of molecular gas. Here, we develop and implement a new recipe for the formation of metal-free Pop III stars. We reach a spatial resolution of 2 pc at z=10 and resolve star-forming galaxies with the masses above 10^6 solar masses. We find the epoch during which Pop III stars dominate the energy and metal budget of the universe to be short-lived. While these stars seed their host galaxies with metals, they cannot drive significant outflows to enrich the IGM in our simulations. Feedback from pair instability supernovae causes Pop III star formation to self-terminate within their host galaxies, but is not strong enough to suppress star formation in external galaxies. Within any individual galaxy, Pop II stars overtake Pop III stars within ~50-150 Myr. A threshold of M = 3 * 10^6 solar masses separates galaxies that lose a significant fraction of their baryons due to Pop III feedback from those that do not. Understanding the nature of the transition between Pop III and Pop II star formation is of key importance for studying the dawn of galaxy formation.

  11. Conformational Dynamics of DNA Repair by Escherichia coli Endonuclease III*

    PubMed Central

    Kuznetsov, Nikita A.; Kladova, Olga A.; Kuznetsova, Alexandra A.; Ishchenko, Alexander A.; Saparbaev, Murat K.; Zharkov, Dmitry O.; Fedorova, Olga S.

    2015-01-01

    Escherichia coli endonuclease III (Endo III or Nth) is a DNA glycosylase with a broad substrate specificity for oxidized or reduced pyrimidine bases. Endo III possesses two types of activities: N-glycosylase (hydrolysis of the N-glycosidic bond) and AP lyase (elimination of the 3′-phosphate of the AP-site). We report a pre-steady-state kinetic analysis of structural rearrangements of the DNA substrates and uncleavable ligands during their interaction with Endo III. Oligonucleotide duplexes containing 5,6-dihydrouracil, a natural abasic site, its tetrahydrofuran analog, and undamaged duplexes carried fluorescent DNA base analogs 2-aminopurine and 1,3-diaza-2-oxophenoxazine as environment-sensitive reporter groups. The results suggest that Endo III induces several fast sequential conformational changes in DNA during binding, lesion recognition, and adjustment to a catalytically competent conformation. A comparison of two fluorophores allowed us to distinguish between the events occurring in the damaged and undamaged DNA strand. Combining our data with the available structures of Endo III, we conclude that this glycosylase uses a multistep mechanism of damage recognition, which likely involves Gln41 and Leu81 as DNA lesion sensors. PMID:25869130

  12. Mechanism of Ribonuclease III Catalytic Regulation by Serine Phosphorylation

    NASA Astrophysics Data System (ADS)

    Gone, Swapna; Alfonso-Prieto, Mercedes; Paudyal, Samridhdi; Nicholson, Allen W.

    2016-05-01

    Ribonuclease III (RNase III) is a conserved, gene-regulatory bacterial endonuclease that cleaves double-helical structures in diverse coding and noncoding RNAs. RNase III is subject to multiple levels of control, reflective of its global regulatory functions. Escherichia coli (Ec) RNase III catalytic activity is known to increase during bacteriophage T7 infection, reflecting the expression of the phage-encoded protein kinase, T7PK. However, the mechanism of catalytic enhancement is unknown. This study shows that Ec-RNase III is phosphorylated on serine in vitro by purified T7PK, and identifies the targets as Ser33 and Ser34 in the N-terminal catalytic domain. Kinetic experiments reveal a 5-fold increase in kcat and a 1.4-fold decrease in Km following phosphorylation, providing a 7.4–fold increase in catalytic efficiency. Phosphorylation does not change the rate of substrate cleavage under single-turnover conditions, indicating that phosphorylation enhances product release, which also is the rate-limiting step in the steady-state. Molecular dynamics simulations provide a mechanism for facilitated product release, in which the Ser33 phosphomonoester forms a salt bridge with the Arg95 guanidinium group, thereby weakening RNase III engagement of product. The simulations also show why glutamic acid substitution at either serine does not confer enhancement, thus underscoring the specific requirement for a phosphomonoester.

  13. Solvolysis of the Tumor-Inhibiting Ru(III)-Complex trans-Tetrachlorobis(Indazole)Ruthenate(III).

    PubMed

    Pieper, T; Peti, W; Keppler, B K

    2000-01-01

    The ruthenium(III) complex Hlnd trans-[RuCl(4),(ind)(2)], with two trans-standing indazole (ind) ligands bound to ruthenium via nitrogen, shows remarkable activity in different tumor models in vitro and in vivo. The solvolysis of the complex trans-[RuCl(4),(ind)(2)](-) has been investigated by means of spectroscopic techniques (UV/vis, NMR)in different solvents. We investigated the indazolium as well as the sodium salt, the latter showing improved solubility in water. In aqueous acetonitrile and ethanol the solvolysis results in one main solvento complex. The hydrolysis of the complex is more complicated and depends on the pH of the solution as well as on the buffer system. PMID:18475949

  14. Form III of 2,2',4,4',6,6'-hexanitroazobenzene (HNAB-III).

    PubMed

    Rodriguez, Mark A; Campana, Charles F; Rae, A David; Graeber, Edward; Morosin, Bruno

    2005-03-01

    The crystal structure of form III of the title compound, HNAB [systematic name: bis(2,4,6-trinitrophenyl)diazene], C12H4N8O12, has finally been solved as a pseudo-merohedral twin (monoclinic space group P2(1), rather than the orthorhombic space group C222(1) suggested by diffraction symmetry) using a dual space recycling method. The significant differences in the room-temperature densities of the three crystalline forms allow examination of molecular differences due to packing arrangements. An interesting relationship with the stilbene analog, HNS, is discussed. Interatomic separations are compared with other explosives and/or nitro-containing compounds. PMID:15750234

  15. Characterisation of serpin polymers in vitro and in vivo.

    PubMed

    Belorgey, Didier; Irving, James A; Ekeowa, Ugo I; Freeke, Joanna; Roussel, Benoit D; Miranda, Elena; Pérez, Juan; Robinson, Carol V; Marciniak, Stefan J; Crowther, Damian C; Michel, Claire H; Lomas, David A

    2011-03-01

    Neuroserpin is a member of the serine protease inhibitor or serpin superfamily of proteins. It is secreted by neurones and plays an important role in the regulation of tissue plasminogen activator at the synapse. Point mutations in the neuroserpin gene cause the autosomal dominant dementia familial encephalopathy with neuroserpin inclusion bodies or FENIB. This is one of a group of disorders caused by mutations in the serpins that are collectively known as the serpinopathies. Others include α(1)-antitrypsin deficiency and deficiency of C1 inhibitor, antithrombin and α(1)-antichymotrypsin. The serpinopathies are characterised by delays in protein folding and the retention of ordered polymers of the mutant serpin within the cell of synthesis. The clinical phenotype results from either a toxic gain of function from the inclusions or a loss of function, as there is insufficient protease inhibitor to regulate important proteolytic cascades. We describe here the methods required to characterise the polymerisation of neuroserpin and draw parallels with the polymerisation of α(1)-antitrypsin. It is important to recognise that the conditions in which experiments are performed will have a major effect on the findings. For example, incubation of monomeric serpins with guanidine or urea will produce polymers that are not found in vivo. The characterisation of the pathological polymers requires heating of the folded protein or alternatively the assessment of ordered polymers from cell and animal models of disease or from the tissues of humans who carry the mutation. PMID:21115126

  16. Complexation of Curium(III) with DTPA at 10–70 °C: Comparison with Eu(III)–DTPA in Thermodynamics, Luminescence, and Coordination Modes

    SciTech Connect

    Tian, Guoxin; Zhang, Zhiyong; Martin, Leigh R.; Rao, Linfeng

    2015-02-16

    Separation of trivalent actinides (An(III)) from trivalent lanthanides (Ln(III)) is a challenging task because of their nearly identical chemical properties. Diethylenetriaminepentaacetate (DTPA), a key reagent used in the TALSPEAK process that effectively separates An(III) from Ln(III), is believed to play a critical role in the An(III)/Ln(III) separation. However, the underlying principles for the separation based on the difference in the complexation of DTPA with An(III) and Ln(III) remain unclear. In this work, the complexation of DTPA with Cm(III) at 10-70 ºC was investigated by spectrophotometry, luminescence spectroscopy, and microcalorimetry, in conjunction with computational methods. The binding strength, the enthalpy of complexation, the coordination modes, and the luminescence properties are compared between the Cm(III)-DTPA and Eu(III)-DTPA systems. The experimental and computational data have demonstrated that the difference between Cm(III) and Eu(III) in the binding strength with DTPA can be attributed to the stronger covalence bonding between Cm(III) and the nitrogen donors of DTPA.

  17. Complexation of Nd(III) with tetraborate ion and its effect on actinide (III) solubility in WIPP brine

    SciTech Connect

    Borkowski, Marian; Richmann, Michael K; Reed, Donald T; Yongliang, Xiong

    2010-01-01

    The potential importance of tetraborate complexation on lanthanide(III) and actinide(III) solubility is recognized in the literature but a systematic study of f-element complexation has not been performed. In neodymium solubility studies in WIPP brines, the carbonate complexation effect is not observed since tetraborate ions form a moderately strong complex with neodymium(III). The existence of these tetraborate complexes was established for low and high ionic strength solutions. Changes in neodymium(III) concentrations in undersaturation experiments were used to determine the neodymium with tetraborate stability constants as a function of NaCl ionic strength. As very low Nd(III) concentrations have to be measured, it was necessary to use an extraction pre-concentration step combined with ICP-MS analysis to extend the detection limit by a factor of 50. The determined Nd(III) with borate stability constants at infinite dilution and 25 C are equal to log {beta}{sub 1} = 4.55 {+-} 0.06 using the SIT approach, equal to log {beta}{sub 1} = 4.99 {+-} 0.30 using the Pitzer approach, with an apparent log {beta}{sub 1} = 4.06 {+-} 0.15 (in molal units) at I = 5.6 m NaCl. Pitzer ion-interaction parameters for neodymium with tetraborate and SIT interaction coefficients were also determined and reported.

  18. Adsorption mechanism of gallium(III) and indium(III) onto {gamma}-Al{sub 2}O{sub 3}

    SciTech Connect

    Lin, C.F.; Tsay, C.W.; Lee, D.Y.; Lo, S.L.; Yasunaga, Tatsuya; Chang, K.S.

    1997-04-01

    The transport of heavy metals in the aquatic environment has long been the primary interest of environmental engineers and geochemists. The adsorption mechanism of trivalent Ga and In onto {gamma}-Al{sub 2}O{sub 3} was investigated using a triple-layer model simulation and pressure-jump technique. Bidentate Ga{sup 3+} and In{sup 3+} and monodentate GaOH{sup 2+}/InOH{sup 2+} are the most likely surface species responsible for Ga(III)/In(III) adsorption. Sorption of Ga(III) and In(III) can be interpreted as an associative process. The adsorption pathway is a two-step mechanism: proton release from surface hydroxyl group(s) followed by coordination of Ga(III)/In(III) species to the depronated site(s). Intrinsic adsorption rate constants cannot be estimated with a liner free-energy relationship between the adsorption rate constant and the rate of water exchange, which is developed solely based on the dissociative sorption mechanism of divalent ions.

  19. Phylogenetic analysis of dissimilatory Fe(III)-reducing bacteria

    USGS Publications Warehouse

    Lonergan, D.J.; Jenter, H.L.; Coates, J.D.; Phillips, E.J.P.; Schmidt, T.M.; Lovley, D.R.

    1996-01-01

    Evolutionary relationships among strictly anaerobic dissimilatory Fe(III)- reducing bacteria obtained from a diversity of sedimentary environments were examined by phylogenetic analysis of 16S rRNA gene sequences. Members of the genera Geobacter, Desulfuromonas, Pelobacter, and Desulfuromusa formed a monophyletic group within the delta subdivision of the class Proteobacteria. On the basis of their common ancestry and the shared ability to reduce Fe(III) and/or S0, we propose that this group be considered a single family, Geobacteraceae. Bootstrap analysis, characteristic nucleotides, and higher- order secondary structures support the division of Geobacteraceae into two subgroups, designated the Geobacter and Desulfuromonas clusters. The genus Desulfuromusa and Pelobacter acidigallici make up a distinct branch with the Desulfuromonas cluster. Several members of the family Geobacteraceae, none of which reduce sulfate, were found to contain the target sequences of probes that have been previously used to define the distribution of sulfate-reducing bacteria and sulfate-reducing bacterium-like microorganisms. The recent isolations of Fe(III)-reducing microorganisms distributed throughout the domain Bacteria suggest that development of 16S rRNA probes that would specifically target all Fe(III) reducers may not be feasible. However, all of the evidence suggests that if a 16S rRNA sequence falls within the family Geobacteraceae, then the organism has the capacity for Fe(III) reduction. The suggestion, based on geological evidence, that Fe(III) reduction was the first globally significant process for oxidizing organic matter back to carbon dioxide is consistent with the finding that acetate-oxidizing Fe(III) reducers are phylogenetically diverse.

  20. Phylogenetic analysis of dissimilatory Fe(III)-reducing bacteria.

    PubMed Central

    Lonergan, D J; Jenter, H L; Coates, J D; Phillips, E J; Schmidt, T M; Lovley, D R

    1996-01-01

    Evolutionary relationships among strictly anaerobic dissimilatory Fe(III)-reducing bacteria obtained from a diversity of sedimentary environments were examined by phylogenetic analysis of 16S rRNA gene sequences. Members of the genera Geobacter, Desulfuromonas, Pelobacter, and Desulfuromusa formed a monophyletic group within the delta subdivision of the class Proteobacteria. On the basis of their common ancestry and the shared ability to reduce Fe(III) and/or S0, we propose that this group be considered a single family, Geobacteraceae. Bootstrap analysis, characteristic nucleotides, and higher-order secondary structures support the division of Geobacteraceae into two subgroups, designated the Geobacter and Desulfuromonas clusters. The genus Desulfuromusa and Pelobacter acidigallici make up a distinct branch within the Desulfuromonas cluster. Several members of the family Geobacteraceae, none of which reduce sulfate, were found to contain the target sequences of probes that have been previously used to define the distribution of sulfate-reducing bacteria and sulfate-reducing bacterium-like microorganisms. The recent isolations of Fe(III)-reducing microorganisms distributed throughout the domain Bacteria suggest that development of 16S rRNA probes that would specifically target all Fe(III) reducers may not be feasible. However, all of the evidence suggests that if a 16S rRNA sequence falls within the family Geobacteraceae, then the organism has the capacity for Fe(III) reduction. The suggestion, based on geological evidence, that Fe(III) reduction was the first globally significant process for oxidizing organic matter back to carbon dioxide is consistent with the finding that acetate-oxidizing Fe(III) reducers are phylogenetically diverse. PMID:8636045

  1. Peroxiredoxin III protects pancreatic ß cells from apoptosis.

    PubMed

    Wolf, Gabriele; Aumann, Nicole; Michalska, Marta; Bast, Antje; Sonnemann, Jürgen; Beck, James F; Lendeckel, Uwe; Newsholme, Philip; Walther, Reinhard

    2010-11-01

    Type 1 diabetes mellitus is characterized by a progressive autoimmune destruction of insulin-producing β cells. Macrophages and T lymphocytes release cytokines, which induce the synthesis of oxygen and nitrogen radicals in the pancreatic islets. The resulting cellular and mitochondrial damage promotes β cell death. β cells are very sensitive to the autoimmune free radical-dependent attack due to their low content of antioxidant enzymes such as glutathione peroxidase and catalase. A focal point of β cell protection should be the control of the mitochondrial redox status, which will result in the preservation of metabolic stimulus-secretion coupling. For this reason, there is a considerable interest in the mitochondrial peroxiredoxin III (PRX III), a thioredoxin-dependent peroxide reductase, which was shown to be able to protect against both oxidative and nitrosative stress. Using the Tet-On-system, we generated stably transfected rat insulinoma cells over- or under-expressing PRX III in a doxycyclin-dependent manner to analyze the effect of increased or decreased amounts of cellular PRX III, following treatment with several stressors. We provide evidence that PRX III protects pancreatic β cells from cell stress induced by accumulation of hydrogen peroxide, or the induction of inducible nitric oxide synthase or caspase-9 and -3 by pro-inflammatory cytokines or streptozotocin. Basal insulin secretion was markedly decreased in cells expressing lower levels of PRX III. We suggest PRX III may be a suitable target for promoting deceleration or even prevention of stress-associated apoptosis in pancreatic β cells and the manifestation of insulin-dependent diabetes mellitus. PMID:20807727

  2. Heterometallic trinuclear {CoLn(III)} (Ln = Gd, Tb, Ho and Er) complexes in a bent geometry. Field-induced single-ion magnetic behavior of the Er(III) and Tb(III) analogues.

    PubMed

    Goura, Joydeb; Brambleby, Jamie; Topping, Craig V; Goddard, Paul A; Suriya Narayanan, Ramakirushnan; Bar, Arun Kumar; Chandrasekhar, Vadapalli

    2016-05-31

    Through the use of a multi-site compartmental ligand, 2-methoxy-6-[{2-(2-hydroxyethylamino)ethylimino}methyl]phenol (LH3), the family of heterometallic, trinuclear complexes of the formula [CoLn(L)2(μ-O2CCH3)2(H2O)3]·NO3·xMeOH·yH2O has been expanded beyond Ln = Dy(III) to include Gd(III) (), Tb(III) (), Ho(III) () and Er(III) () for , and (x = 1; y = 1) and for (x = 0; y = 2). The metallic core of these complexes consists of a (Co(III)-Ln(III)-Co(III)) motif bridged in a bent geometry resulting in six-coordinated distorted Co(III) octahedra and nine-coordinated Ln(III) monocapped square-antiprisms. The magnetic characterization of these compounds reveals the erbium and terbium analogues to display a field induced single-ion magnetic behavior similar to the dysprosium analogue but at lower temperatures. The energy barrier for the reversal of the magnetization of the CoTb(III) analogue is Ueff ≥ 15.6(4) K, while for the CoEr(III) analogue Ueff ≥ 9.9(8) K. The magnetic properties are discussed in terms of distortions of the 4f electron cloud. PMID:27180723

  3. Characterization of Fe (III)-reducing enrichment culture and isolation of Fe (III)-reducing bacterium Enterobacter sp. L6 from marine sediment.

    PubMed

    Liu, Hongyan; Wang, Hongyu

    2016-07-01

    To enrich the Fe (III)-reducing bacteria, sludge from marine sediment was inoculated into the medium using Fe (OH)3 as the sole electron acceptor. Efficiency of Fe (III) reduction and composition of Fe (III)-reducing enrichment culture were analyzed. The results indicated that the Fe (III)-reducing enrichment culture with the dominant bacteria relating to Clostridium and Enterobacter sp. had high Fe (III) reduction of (2.73 ± 0.13) mmol/L-Fe (II). A new Fe (III)-reducing bacterium was isolated from the Fe (III)-reducing enrichment culture and identified as Enterobacter sp. L6 by 16S rRNA gene sequence analysis. The Fe (III)-reducing ability of strain L6 under different culture conditions was investigated. The results indicated that strain L6 had high Fe (III)-reducing activity using glucose and pyruvate as carbon sources. Strain L6 could reduce Fe (III) at the range of NaCl concentrations tested and had the highest Fe (III) reduction of (4.63 ± 0.27) mmol/L Fe (II) at the NaCl concentration of 4 g/L. This strain L6 could reduce Fe (III) with unique properties in adaptability to salt variation, which indicated that it can be used as a model organism to study Fe (III)-reducing activity isolated from marine environment. PMID:26896316

  4. III-Nitride Vertical-Cavity Surface-Emitting Lasers

    NASA Astrophysics Data System (ADS)

    Leonard, John T.

    Vertical-cavity surface-emitting lasers (VCSELs) have a long history of development in GaAs-based and InP-based systems, however III-nitride VCSELs research is still in its infancy. Yet, over the past several years we have made dramatic improvements in the lasing characteristics of these highly complex devices. Specifically, we have reduced the threshold current density from ˜100 kA/cm2 to ˜3 kA/cm2, while simultaneously increasing the output power from ˜10 muW to ˜550 muW. These developments have primarily come about by focusing on the aperture design and intracavity contact design for flip-chip dual dielectric DBR III-nitride VCSELs. We have carried out a number of studies developing an Al ion implanted aperture (IIA) and photoelectrochemically etched aperture (PECA), while simultaneously improving the quality of tin-doped indium oxide (ITO) intracavity contacts, and demonstrating the first III-nitride VCSEL with an n-GaN tunnel junction intracavity contact. Beyond these most notable research fronts, we have analyzed numerous other parameters, including epitaxial growth, flip-chip bonding, substrate removal, and more, bringing further improvement to III-nitride VCSEL performance and yield. This thesis aims to give a comprehensive discussion of the relevant underlying concepts for nonpolar VCSELs, while detailing our specific experimental advances. In Section 1, we give an overview of the applications of VCSELs generally, before describing some of the potential applications for III-nitride VCSELs. This is followed by a summary of the different material systems used to fabricate VCSELs, before going into detail on the basic design principles for developing III-nitride VCSELs. In Section 2, we outline the basic process and geometry for fabricating flip-chip nonpolar VCSELs with different aperture and intracavity contact designs. Finally, in Section 3 and 4, we delve into the experimental results achieved in the last several years, beginning with a discussion on

  5. Heterogeneity of collagens in rabbit cornea: type III collagen

    SciTech Connect

    Cintron, C.; Hong, B.S.; Covington, H.I.; Macarak, E.J.

    1988-05-01

    Whole neonate rabbit corneas and adult corneas containing 2-week-old scars were incubated in the presence of (/sup 14/C) glycine. Radiolabeled collagen extracted from the corneas and scar tissue were analyzed by sodium dodecylsulfate/polyacrylamide gel electrophoresis and fluorography to determine the types and relative quantity of collagen polypeptides present and synthesized by these tissues. In addition to other collagen types, type III was found in both neonate cornea and scar tissue from adult cornea, albeit in relatively small quantities. Type III collagen in normal cornea was associated with the residue after pepsin digestion and formic acid extraction of the tissue, and the same type of collagen was extracted from scar tissue after similar treatment. Type III collagen-specific monoclonal antibody bound to developing normal corneas and healing adult tissue sections, as determined by immunofluorescence. Antibody binding was localized to the endothelium and growing Descemet's membrane in fetal and neonate corneas, and restricted to the most posterior region of the corneal scar tissue. Although monoclonal antibody to keratan sulfate, used as a marker for stromal fibroblasts, bound to most of the scar tissue, the antibody failed to bind to the posterior scar tissue positive for type III collagen. We conclude that endothelial cells from fetal and neonate rabbit cornea and endothelium-derived fibroblasts from healing wounds of adult cornea synthesize and deposit type III collagen. Moreover, this collagen appears to be incorporated into the growing Descemet's membrane of normal corneas and narrow posterior portion of the scar tissue.

  6. The III-V photocathode - A major detector development.

    NASA Technical Reports Server (NTRS)

    Spicer, W. E.; Bell, R. L.

    1972-01-01

    Description of new (III-V) photocathodes which show improvements in sensitivity of as much as ten to a hundred times over conventional cathodes in the near infrared and useful improvements at shorter wavelengths. The development stems from a combination of basic knowledge of the photoemission process, gained in the 1950s, and the advancing understanding of the technology of III-V materials, in the 1960s. The superior performance of these cathodes is due to the fact that the vacuum level at the surface lies below the bottom of the conduction band in the bulk of the material. Consequently, the threshold of response is set by the III-V bandgap. The bandgap (and the threshold of response) can be varied by alloying different III-V materials together. A reduction in thermionic emission is realized with these cathodes. At present no semitransparent III-V cathodes with comparably interesting performance are available. The problems hindering further improvements, as well as the problems of placing these cathodes in practical multipliers and image tubes, are discussed briefly.

  7. Structural and electronic properties of III-V bismuth compounds

    NASA Astrophysics Data System (ADS)

    Ferhat, M.; Zaoui, A.

    2006-03-01

    We have performed ab initio self-consistent calculations based on the full potential linear augmented plane-wave method with the generalized gradient approximation to investigate the structural and the electronic properties of the less known bismuth III-V compounds: BBi, AlBi, GaBi, and InBi. Ground state parameters are computed and compared with available theoretical and experimental works. The zinc-blende phase is found to be the most stable for BBi, AlBi, and GaBi, while InBi prefers the tetragonal PbO structure. The relativistic contraction of the 6s orbital of Bi has strong effect on the band structure of III-Bi compounds, which exhibits some features that differ considerably from those of typical III-V semiconductors. In particular, we found an inverted band gap, which reflects a semimetallic character of these systems. Their bonding nature is analyzed in terms of valence charge density transfer, showing three different natures of the bond. Besides, the calculated valence charge density for BBi shows an anomalous behavior characterized by a charge transfer toward the cation B atom, while the others III-Bi behave as the typical III-V compounds with a small charge transfer to the anion bismuth atom.

  8. A well-defined terminal vanadium(III) oxo complex.

    PubMed

    King, Amanda E; Nippe, Michael; Atanasov, Mihail; Chantarojsiri, Teera; Wray, Curtis A; Bill, Eckhard; Neese, Frank; Long, Jeffrey R; Chang, Christopher J

    2014-11-01

    The ubiquity of vanadium oxo complexes in the V+ and IV+ oxidation states has contributed to a comprehensive understanding of their electronic structure and reactivity. However, despite being predicted to be stable by ligand-field theory, the isolation and characterization of a well-defined terminal mononuclear vanadium(III) oxo complex has remained elusive. We present the synthesis and characterization of a unique terminal mononuclear vanadium(III) oxo species supported by the pentadentate polypyridyl ligand 2,6-bis[1,1-bis(2-pyridyl)ethyl]pyridine (PY5Me2). Exposure of [V(II)(NCCH3)(PY5Me2)](2+) (1) to either dioxygen or selected O-atom-transfer reagents yields [V(IV)(O)(PY5Me2)](2+) (2). The metal-centered one-electron reduction of this vanadium(IV) oxo complex furnishes a stable, diamagnetic [V(III)(O)(PY5Me2)](+) (3) species. The vanadium(III) oxo species is unreactive toward H- and O-atom transfer but readily reacts with protons to form a putative vanadium hydroxo complex. Computational results predict that further one-electron reduction of the vanadium(III) oxo species will result in ligand-based reduction, even though pyridine is generally considered to be a poor π-accepting ligand. These results have implications for future efforts toward low-valent vanadyl chemistry, particularly with regard to the isolation and study of formal vanadium(II) oxo species. PMID:25097094

  9. Leica Dmc III Calibration and Geometric Sensor Accuracy

    NASA Astrophysics Data System (ADS)

    Mueller, C.; Neumann, K.

    2016-03-01

    As an evolution of the successful DMC II digital camera series, Leica Geosystems has introduced the Leica DMC III digital aerial camera using, for the first time in the industry, a large-format CMOS sensor as a panchromatic high-resolution camera head. This paper describes the Leica DMC III calibration and its quality assurance and quality control (QA/QC) procedures. It will explain how calibration was implemented within the production process for the Leica DMC III camera. Based on many years of experience with the DMC and DMC II camera series, it is know that the sensor flatness has a huge influence on the final achievable results. The Leica DMC III panchromatic CMOS sensor with its 100.3mm x 56.9mm size shows remaining errors in a range of 0.1 to 0.2μm for the root mean square and shows maximum values not higher that 1.0μm. The Leica DMC III is calibrated based on a 5cm Ground Sample Distance (GSD) grid pattern flight and evaluated with three different flying heights at 5cm, 8cm and 11cm GSD. The geometric QA/QC has been performed using the calibration field area, as well as using an independent test field. The geometric performance and accuracy is unique and gives ground accuracies far better than the flown GSD.

  10. III-V aresenide-nitride semiconductor materials and devices

    NASA Technical Reports Server (NTRS)

    Major, Jo S. (Inventor); Welch, David F. (Inventor); Scifres, Donald R. (Inventor)

    1997-01-01

    III-V arsenide-nitride semiconductor crystals, methods for producing such crystals and devices employing such crystals. Group III elements are combined with group V elements, including at least nitrogen and arsenic, in concentrations chosen to lattice match commercially available crystalline substrates. Epitaxial growth of these III-V crystals results in direct bandgap materials, which can be used in applications such as light emitting diodes and lasers. Varying the concentrations of the elements in the III-V crystals varies the bandgaps, such that materials emitting light spanning the visible spectra, as well as mid-IR and near-UV emitters, can be created. Conversely, such material can be used to create devices that acquire light and convert the light to electricity, for applications such as full color photodetectors and solar energy collectors. The growth of the III-V crystals can be accomplished by growing thin layers of elements or compounds in sequences that result in the overall lattice match and bandgap desired.

  11. Possible Population III remnants at redshift 3.5

    NASA Astrophysics Data System (ADS)

    Crighton, Neil H. M.; O'Meara, John M.; Murphy, Michael T.

    2016-03-01

    The first stars, known as Population III (PopIII), produced the first heavy elements, thereby enriching their surrounding pristine gas. Previous detections of metals in intergalactic gas clouds, however, find a heavy element enrichment larger than 1/1000 times that of the solar environment, higher than expected for PopIII remnants. In this letter, we report the discovery of a Lyman limit system (LLS) at z = 3.53 with the lowest metallicity seen in gas with discernable metals, 10-3.41±0.26 times the solar value, at a level expected for PopIII remnants. We make the first relative abundance measurement in such low metallicity gas: the carbon-to-silicon ratio is 10-0.26±0.17 times the solar value. This is consistent with models of gas enrichment by a PopIII star formation event early in the Universe, but also consistent with later, Population II enrichment. The metals in all three components comprising the LLS, which has a velocity width of 400 km s-1, are offset in velocity by ˜+6 km s-1 from the bulk of the hydrogen, suggesting the LLS was enriched by a single event. Relative abundance measurements in this near-pristine regime open a new avenue for testing models of early gas enrichment and metal mixing.

  12. 12 CFR Appendix III to Part 27 - Fair Housing Lending Inquiry/Application Log Sheet

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Fair Housing Lending Inquiry/Application Log Sheet III Appendix III to Part 27 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY FAIR HOUSING HOME LOAN DATA SYSTEM Pt. 27, App. III Appendix III to Part 27—Fair Housing...

  13. 17 CFR Table III to Subpart E of... - Civil Monetary Penalty Inflation Adjustments

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Civil Monetary Penalty Inflation Adjustments III Table III to Subpart E of Part 201 Commodity and Securities Exchanges SECURITIES..., Table III Table III to Subpart E of Part 201—Civil Monetary Penalty Inflation Adjustments U.S....

  14. 46 CFR Appendix III to Part 150 - Testing Procedures for Determining Exceptions to the Chart

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 5 2013-10-01 2013-10-01 false Testing Procedures for Determining Exceptions to the Chart III Appendix III to Part 150 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES Pt. 150, App. III Appendix III to Part 150—Testing Procedures for Determining Exceptions to...

  15. 19 CFR Annex III to Part 351 - Deadlines for Parties in Antidumping Investigations

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 3 2011-04-01 2011-04-01 false Deadlines for Parties in Antidumping Investigations III Annex III to Part 351 Customs Duties INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE ANTIDUMPING AND COUNTERVAILING DUTIES Pt. 351, Annex III Annex III to Part 351—Deadlines...

  16. 19 CFR Annex III to Part 351 - Deadlines for Parties in Antidumping Investigations

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Deadlines for Parties in Antidumping Investigations III Annex III to Part 351 Customs Duties INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE ANTIDUMPING AND COUNTERVAILING DUTIES Pt. 351, Annex III Annex III to Part 351—Deadlines...

  17. 40 CFR Appendix III to Part 600 - Sample Fuel Economy Label Calculation

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 30 2011-07-01 2011-07-01 false Sample Fuel Economy Label Calculation III Appendix III to Part 600 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) ENERGY POLICY FUEL ECONOMY AND CARBON-RELATED EXHAUST EMISSIONS OF MOTOR VEHICLES Pt. 600, App. III Appendix III to Part 600—Sample Fuel Economy...

  18. 40 CFR Appendix III to Part 600 - Sample Fuel Economy Label Calculation

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 31 2012-07-01 2012-07-01 false Sample Fuel Economy Label Calculation III Appendix III to Part 600 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) ENERGY POLICY FUEL ECONOMY AND GREENHOUSE GAS EXHAUST EMISSIONS OF MOTOR VEHICLES Pt. 600, App. III Appendix III to Part 600—Sample Fuel Economy...

  19. 40 CFR Appendix III to Part 600 - Sample Fuel Economy Label Calculation

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 31 2013-07-01 2013-07-01 false Sample Fuel Economy Label Calculation III Appendix III to Part 600 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) ENERGY POLICY FUEL ECONOMY AND GREENHOUSE GAS EXHAUST EMISSIONS OF MOTOR VEHICLES Pt. 600, App. III Appendix III to Part 600—Sample Fuel Economy...

  20. 17 CFR Table III to Subpart E of... - Civil Monetary Penalty Inflation Adjustments

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 2 2011-04-01 2011-04-01 false Civil Monetary Penalty Inflation Adjustments III Table III to Subpart E of Part 201 Commodity and Securities Exchanges SECURITIES..., Table III Table III to Subpart E of Part 201—Civil Monetary Penalty Inflation Adjustments U.S....