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Sample records for 1h-31 cross-polarization edited

  1. A practical guide for the setup of a 1H-31P-13C double cross-polarization (DCP) experiment.

    PubMed

    Ciesielski, Wlodzimierz; Kassassir, Hassan; Potrzebowski, Marek J

    2011-01-01

    O-phospho-L-threonine is a convenient sample to setup a (1)H-(31)P-(13)C double cross-polarization (DCP) Hartmann-Hahn match. The (1)H-(31)P-(13)C technique is extremely sensitive to the rate of the sample spinning. Both zero-quantum (ZQ) and double-quantum (DQ) cross-polarization operate at an average spinning rate (6-7 kHz). At higher spinning rates (10 kHz), the DQCP mechanism dominates and leads to a reduction of signal intensity, in particular for lower (31)P RF field strength. The application of two shape pulses during the second cross-polarization greatly improves the signal to noise ratio allowing the recording of better quality spectra. (31)P-(13)C spectrally induced filtering in combination with cross-polarization (SPECIFIC-CP) experiments can be carried out under ZQCP and DQCP conditions if careful attention is paid to the choice of RF field amplitudes and carriers Ω. Application of 1D and 2D (1)H-(31)P-(13)C experiments is demonstrated on model samples; disodium ATP hydrate and O-phospho-L-tyrosine. PMID:21440422

  2. Optimized Spectral Editing of 13C MAS NMR Spectra of Rigid Solids Using Cross-Polarization Methods

    NASA Astrophysics Data System (ADS)

    Sangill, R.; Rastrupandersen, N.; Bildsoe, H.; Jakobsen, H. J.; Nielsen, N. C.

    Combinations of 13C magic-angle spinning (MAS) NMR experiments employing cross polarization (CP), cross polarization-depolarization (CPD), and cross polarization-depolarization-repolarization are analyzed quantitatively to derive simple and general procedures for optimized spectral editing of 13C CP/MAS NMR spectra of rigid solids by separation of the 13C resonances into CH n subspectra ( n = 0, 1, 2, and 3). Special attention is devoted to a differentiation by CPD/MAS of CH and CH 2 resonances since these groups behave quite similarly during spin lock under Hartmann-Hahn match and are therefore generally difficult to distinguish unambiguously. A general procedure for the design of subexperiments and linear combinations of their spectra to provide optimized signal-to-noise ratios for the edited subspectra is described. The technique is illustrated by a series of edited 13C CP/MAS spectra for a number of rigid solids ranging from simple organic compounds (sucrose and l-menthol) to complex pharmaceutical products (calcipotriol monohydrate and vitamin D 3) and polymers (polypropylene, polyvinyl alcohol, polyvinyl chloride, and polystyrene).

  3. Cross polarization compatible dialysis chip.

    PubMed

    Kornreich, Micha; Heymann, Michael; Fraden, Seth; Beck, Roy

    2014-10-01

    We visualize birefringence in microliter sample volumes using a microfluidic dialysis chip optimized for cross polarization microscopy. The chip is composed of two overlapping polydimethylsiloxane (PDMS) channels separated by a commercial cellulose ester membrane. Buffer exchange in the sample chamber is achieved within minutes by dialyzing under continuous reservoir flow. Using fd virus as a birefringent model system, we monitor the fd virus isotropic to liquid crystal phase transition as a function of ionic strength. We show that the reorientation of the fd virus spans a few tens of seconds, indicative of fast ion exchange across the membrane. Complete phase separation reorganization takes minutes to hours as it involves diffusive virus mass transport within the storage chamber. PMID:25105977

  4. Cross-polarized excitons in carbon nanotubes.

    PubMed

    Kilina, Svetlana; Tretiak, Sergei; Doorn, Stephen K; Luo, Zhengtang; Papadimitrakopoulos, Fotios; Piryatinski, Andrei; Saxena, Avadh; Bishop, Alan R

    2008-05-13

    Polarization of low-lying excitonic bands in finite-size semiconducting single-walled carbon nanotubes (SWNTs) is studied by using quantum-chemical methodologies. Our calculations elucidate properties of cross-polarized excitons, which lead to the transverse optical absorption of nanotubes and presumably couple to intermediate-frequency modes recently observed in resonance Raman excitation spectroscopy. We identify up to 12 distinct excitonic transitions below the second fundamental band associated with the E(22) van Hove singularity. Calculations for several chiral SWNTs distinguish the optically active "bright" excitonic band polarized parallel to the tube axis and several optically "weak" cross-polarized excitons. The rest are optically (near) forbidden "dark" transitions. An analysis of the transition density matrices related to excitonic bands provides detailed information about delocalization of excitonic wavefunction along the tube. Utilization of the natural helical coordinate system accounting for the tube chirality allows one to disentangle longitudinal and circumferential components. The distribution of the transition density matrix along a tube axis is similar for all excitons. However, four parallel-polarized excitons associated with the E(11) transition are more localized along the circumference of a tube, compared with others related to the E(12) and E(21) cross-polarized transitions. Calculated splitting between optically active parallel- and cross-polarized transitions increases with tube diameter, which compares well with experimental spectroscopic data. PMID:18463293

  5. Cross-polarized excitons in carbon nanotubes

    PubMed Central

    Kilina, Svetlana; Tretiak, Sergei; Doorn, Stephen K.; Luo, Zhengtang; Papadimitrakopoulos, Fotios; Piryatinski, Andrei; Saxena, Avadh; Bishop, Alan R.

    2008-01-01

    Polarization of low-lying excitonic bands in finite-size semiconducting single-walled carbon nanotubes (SWNTs) is studied by using quantum-chemical methodologies. Our calculations elucidate properties of cross-polarized excitons, which lead to the transverse optical absorption of nanotubes and presumably couple to intermediate-frequency modes recently observed in resonance Raman excitation spectroscopy. We identify up to 12 distinct excitonic transitions below the second fundamental band associated with the E22 van Hove singularity. Calculations for several chiral SWNTs distinguish the optically active “bright” excitonic band polarized parallel to the tube axis and several optically “weak” cross-polarized excitons. The rest are optically (near) forbidden “dark” transitions. An analysis of the transition density matrices related to excitonic bands provides detailed information about delocalization of excitonic wavefunction along the tube. Utilization of the natural helical coordinate system accounting for the tube chirality allows one to disentangle longitudinal and circumferential components. The distribution of the transition density matrix along a tube axis is similar for all excitons. However, four parallel-polarized excitons associated with the E11 transition are more localized along the circumference of a tube, compared with others related to the E12 and E21 cross-polarized transitions. Calculated splitting between optically active parallel- and cross-polarized transitions increases with tube diameter, which compares well with experimental spectroscopic data. PMID:18463293

  6. Ultra-broadband perfect cross polarization conversion metasurface

    NASA Astrophysics Data System (ADS)

    Dong, Guoxiang; Shi, Hongyu; Xia, Song; Zhang, Anxue; Xu, Zhuo; Wei, Xiaoyong

    2016-04-01

    We propose a metasurface with multiple plasmon resonances that achieves an ultra-broadband perfect cross polarization conversion. The metasurface is composed of an array of unit resonators, three plasmon resonances are excited in the unit resonator, which leads to an ultra-broadband perfect cross polarization conversion. The cross polarization conversion efficiency is higher than 99%, and the bandwidth of the converter is 53.7% of the central wavelength. Both numerical and experimental results were used to validate the ultra-broadband perfect cross polarization converter presented here.

  7. Cross polarization in beam waveguide-fed Cassegrain reflector antennas

    NASA Astrophysics Data System (ADS)

    Houshmand, Bijan

    1991-02-01

    The sensitivity of the cross-polarization level to a deviation from the geometrical condition derived by Mizusawa and Kitsuregawa (1973) for different geometrical configurations is studied. This condition restricts the number of possible beam waveguide configurations for beam waveguide-fed Cassegrain reflector (BFCR) antennas. For a symmetrical feed, this condition results in a symmetrical aperture distribution with no cross-polarized component. By examining a number of beam waveguide configurations satisfying the condition, it was observed that for linearly polarized feed, the cross-polarization level is very sensitive to a deviation from this condition. For circularly polarized feed, deviation from this condition does not increase the cross-polarization level; however, it results in the squinting of the beam for BFCRs.

  8. Reducing Cross-Polarized Radiation From A Microstrip Antenna

    NASA Technical Reports Server (NTRS)

    Huang, John

    1991-01-01

    Change in configuration of feed of nominally linearly polarized microstrip-patch transmitting array antenna reduces cross-polarized component of its radiation. Patches fed on opposing sides, in opposite phases. Combination of spatial symmetry and temporal asymmetry causes copolarized components of radiation from fundamental modes of patches to reinforce each other and cross-polarized components of radiation from higher-order modes to cancel each other.

  9. Structural characterization of chemical warfare agent degradation products in decontamination solutions with proton band-selective (1)H-(31)P NMR spectroscopy.

    PubMed

    Koskela, Harri; Hakala, Ullastiina; Vanninen, Paula

    2010-06-15

    Decontamination solutions, which are usually composed of strong alkaline chemicals, are used for efficient detoxification of chemical warfare agents (CWAs). The analysis of CWA degradation products directly in decontamination solutions is challenging due to the nature of the matrix. Furthermore, occasionally an unforeseen degradation pathway can result in degradation products which could be eluded to in standard analyses. Here, we present the results of the application of proton band-selective (1)H-(31)P NMR spectroscopy, i.e., band-selective 1D (1)H-(31)P heteronuclear single quantum coherence (HSQC) and band-selective 2D (1)H-(31)P HSQC-total correlation spectroscopy (TOCSY), for ester side chain characterization of organophosphorus nerve agent degradation products in decontamination solutions. The viability of the approach is demonstrated with a test mixture of typical degradation products of nerve agents sarin, soman, and VX. The proton band-selective (1)H-(31)P NMR spectroscopy is also applied in characterization of unusual degradation products of VX in GDS 2000 solution. PMID:20507069

  10. Fracture Flow Channel Imaging Using Cross-Polarized GPR Signals

    NASA Astrophysics Data System (ADS)

    Tsoflias, G. P.; Perll, C.; Baker, M.; Becker, M.

    2014-12-01

    Fractures control the flow of fluids in rocks with important implications for groundwater resources, contaminant transport, geothermal resources, sequestration of carbon dioxide, and the development of unconventional hydrocarbon resources. Ground penetrating radar (GPR) can be used to image fractured rock and monitor the flow of fluids in the subsurface. Conventional GPR imaging uses single-polarization, co-polarized signals. Changes in reflected signal amplitude result from changes in fracture aperture and changes in fluid electrical properties introduced by tracers or contaminants. Recent research has also shown changes in radar reflected signal phase resulting from changes in fluid electrical conductivity. However, the radar response is dependent on the polarization of EM waves. This study investigates the use of cross-polarized GPR signals for imaging flow channeling at a discrete horizontal fracture. Numerical modeling demonstrated that cross-polarized GPR data are able to image fracture channels when the axis of the channel is oriented obliquely to the EM wavefield orientation. Summation of the cross-polarized and co-polarized components results in an accurate representation of the total scattered energy from the channel. Multipolarization, time-lapse 3D GPR field data were used investigate GPR imaging of flow channeling in a discrete subhorizontal fracture. The GPR surveys were conducted during background fresh fracture water conditions and during six varying orientation dipole flow saline tracer tests. The cross-polarized data revealed flow channeling which is in agreement with the co-polarized GPR data and with independent hydraulic tests. In addition, the cross-polarized components showed changes in flow channeling as a result of changing dipole flow orientation and position. This study demonstrates that cross-polarized GPR signals can be used to enhance imaging of flow in fractured rock.

  11. Coherent Backscattering in the Cross-Polarized Channel

    NASA Technical Reports Server (NTRS)

    Mischenko, Michael I.; Mackowski, Daniel W.

    2011-01-01

    We analyze the asymptotic behavior of the cross-polarized enhancement factor in the framework of the standard low-packing-density theory of coherent backscattering by discrete random media composed of spherically symmetric particles. It is shown that if the particles are strongly absorbing or if the smallest optical dimension of the particulate medium (i.e., the optical thickness of a plane-parallel slab or the optical diameter of a spherically symmetric volume) approaches zero, then the cross-polarized enhancement factor tends to its upper-limit value 2. This theoretical prediction is illustrated using direct computer solutions of the Maxwell equations for spherical volumes of discrete random medium.

  12. Spatio-Temporal Dynamics of Cross Polarized Wave Generation

    NASA Astrophysics Data System (ADS)

    Adams, Daniel; Squier, Jeff; Durfee, Charles

    2009-10-01

    We use time-domain Spatially and Spectrally Resolved Interferometry (SSRI) to investigate cross-polarized wave (XPW) generation in barium fluoride. We find that the XPW pulse is √3 smaller than the input in the spatiotemporal domain regardless of the input chirp. Additionally, we calculate a temporally dependent focal length resulting from the nonlinear interaction, and discuss its implications.

  13. Cross polarization with long delayed contact in rotating solids

    NASA Astrophysics Data System (ADS)

    Ding, Shangwu; McDowell, Charles A.

    1996-06-01

    The cross polarization NMR spectra and cross relaxation rates of polycrystalline hexamethylbenzene and adamantane were obtained by employing a long delay before contact for samples spinning at, and off, the magic angle with respect to the static magnetic field. The results are compared with those obtained by using the conventional cross polarization pulse sequence. The results support the memory effect explanation of origin of the SPEDA spectra in contrast with the assumptions that the high resolution SPEDA spectra arising either, from the nearly isotropic motion of a very small fraction, or a pool of highly thermo-activated molecules, or from special spin pairs within the crystallites which are orientated at the magic angle with respect to the static magnetic field.

  14. NMR cross polarization in solids using multipulse irradiation

    NASA Astrophysics Data System (ADS)

    Quiroga, L.; Virlet, J.

    1984-12-01

    A double resonance NMR scheme (MPSLCP) is presented in which a WHH-4 type multipulse irradiation of the abundant spins I is applied during cross polarization. When using the HW-8 sequence, the locking Hamiltonian for the I spins is provided by the average pulse length error Hamiltonian. Double resonance is obtained with a weak pulse irradiation of the rare spin S, whose amplitude matches (Hartmann-Hahn condition) the average pulse error field. Experimental results on 13C-1H in powdered adamantane are presented. Owing to the high symmetry of both the crystal and the molecular reorientations, the 13C multipulse cross-polarization rise is described by considering one carbon coupled to N equivalent protons and applying the calculations of L. Muller and R. R. Ernst [Mol. Phys. 38, 963 (1979)] for an SIN system with, here N=196 (or better N=235). Such a cross-polarization scheme (MPSLCP) is shown to be applicable to rigid organic solids. For S spins with very low gyromagnetic ratio and/or far from I spins, MPSLCP (i) is selective; (ii) increases the rate of polarization (it is suggested that this might help for quantitative analysis purposes); (iii) provides a very important rf power saving (104 here for adamantane) at the S spin frequency.

  15. Tunable graphene-based dual-frequency cross polarization converters

    NASA Astrophysics Data System (ADS)

    Ding, Jun; Arigong, Bayaner; Ren, Han; Shao, Jin; Zhou, Mi; Lin, Yuankun; Zhang, Hualiang

    2015-03-01

    In this paper, we proposed a novel cross-polarization converter that simultaneously works at two frequencies in the reflection mode, which is constructed of an L-shape perforated graphene sheet printed on a dielectric spacer backed by a gold layer. For the normal incidence, the optical rotation at these two working frequencies originates from the simultaneous excitation of both eigenmodes characterized as the localized surface plasmon resonances. In addition, both working frequencies can be tuned within a large frequency range by varying the Fermi energy of the graphene, which opens up tremendous opportunities to develop voltage-controlled tunable devices at mid-IR frequencies.

  16. Cross-polarized interferometry of a Jovian decametric radio storm

    NASA Astrophysics Data System (ADS)

    Phillips, J. A.; Carr, T. D.; Greenman, W. B.; Levy, J.

    1987-09-01

    Observations of an 18-MHz Io-A storm have been conducted using a 46 km cross-polarized interferometer at the University of Florida. The pre- and postdetection correlation properties of LH and RH elliptically polarized L bursts have been studied. Fringe visibility measurements suggest that two discrete and independent sources are sometimes simultaneously active, the polarization sense of one being LH and that of the other RH. A scintillation analysis supports the conclusion that two or more sources were active in such cases.

  17. Cross-polarization for dissolution dynamic nuclear polarization.

    PubMed

    Batel, Michael; Däpp, Alexander; Hunkeler, Andreas; Meier, Beat H; Kozerke, Sebastian; Ernst, Matthias

    2014-10-21

    Dynamic nuclear polarization (DNP) in combination with subsequent dissolution of the sample allows the detection of low-γ nuclei in the solution state with a signal gain of up to tens of thousand times compared to experiments starting from Boltzmann conditions. The long polarization build-up times of typically more than one hour are a drawback of this technique. The combination of dissolution DNP with cross-polarization (CP) in the solid state was shown to have the potential to overcome this disadvantage. In this article we discuss the cross-polarization step under dissolution DNP conditions in more detail. We show that adiabatic half-passage pulses allow us to enhance the CP efficiency in power-limited DNP probes. As a low-power alternative to Hartmann-Hahn CP we also demonstrate the applicability of frequency-swept de- and re-magnetization pulses for polarization transfer via dipolar order. We investigate the implications and restrictions of the common solid-state DNP mechanisms to the DNP-CP technique and apply a spin-thermodynamic model based on the thermal-mixing mechanism. The model allows us to investigate the dynamics of the polarization levels in a system with two nuclear Zeeman reservoirs and explains the enhanced DNP efficiency upon solvent deuteration within a spin-thermodynamic picture. PMID:25182534

  18. Cross Polarization for 1H NMR Image Contrast in Solids

    NASA Astrophysics Data System (ADS)

    Nakai, Toshihito; Fukunaga, Yasuhiro; Nonaka, Masayuki; Matsui, Shigeru; Inouye, Tamon

    1998-09-01

    A novel1H imaging method for solids, yielding images reflecting1H-13C dipolar interactions through cross relaxation timeTIS, is presented. Phase-alternating multiple-contact cross polarization (PAMC CP) was incorporated into the magic-echo frequency-encoding imaging scheme; the PAMC CP sequence may partly but efficiently destroy the initial1H magnetization depending on theTISvalues. A theory describing the effects of the PAMC CP sequence was developed, which was used for the assessment of the sequence as well as the analysis for the experimental results. It was demonstrated that theTIS-weighted1H image and theTISmapping for a phantom, constituted of adamantane and ferrocene, can distinguish these compounds clearly.

  19. Cross-Polar Aircraft Trajectory Optimization and Potential Climate Impact

    NASA Technical Reports Server (NTRS)

    Sridhar, Banavar; Chen, Neil; Ng, Hok

    2011-01-01

    Cross-Polar routes offer new opportunities for air travel markets. Transpolar flights reduce travel times, fuel burns, and associated environmental emissions by flying direct paths between many North American and Asian cities. This study evaluates the potential benefits of flying wind-optimal polar routes and assessed their potential impact on climate change. An optimization algorithm is developed for transpolar flights to generate wind-optimal trajectories that minimize climate impact of aircraft, in terms of global warming potentials (relative to warming by one kg of CO2) of several types of emissions, while avoiding regions of airspace that facilitate persistent contrail formation. Estimations of global warming potential are incorporated into the objective function of the optimization algorithm to assess the climate impact of aircraft emissions discharged at a given location and altitude. The regions of airspace with very low ambient temperature and areas favorable to persistent contrail formation are modeled as undesirable regions that aircraft should avoid and are formulated as soft state constraints. The fuel burn and climate impact of cross-polar air traffic flying various types of trajectory including flightplan, great circle, wind-optimal, and contrail-avoidance are computed for 15 origin-destination pairs between major international airports in the U.S. and Asia. Wind-optimal routes reduce average fuel burn of flight plan routes by 4.4% on December 4, 2010 and 8.0% on August 7, 2010, respectively. The tradeoff between persistent contrail formation and additional global warming potential of aircraft emissions is investigated with and without altitude optimization. Without altitude optimization, the reduction in contrail travel times is gradual with increase in total fuel consumption. When altitude is optimized, a one percent increase in additional global warming potential, a climate impact equivalent to that of 4070kg and 4220kg CO2 emission, reduces 135

  20. Dynamic interleaved 1H/31P STEAM MRS at 3 Tesla using a pneumatic force-controlled plantar flexion exercise rig

    PubMed Central

    Meyerspeer, M.; Krššák, M.; Kemp, G.J.; Roden, M.; Moser, E.

    2016-01-01

    1 Objective To develop a measurement method for interleaved acquisition of 1H and 31P STEAM localised spectra of exercising human calf muscle. 2 Materials and Methods A nonmagnetic exercise rig with a pneumatic piston and sensors for force and pedal angle was constructed to enable plantar flexion measured in the 3 Tesla MR scanner, which holds the dual tuned (1H,31P) surface coil used for signal transmission and reception. 3 Results 31P spectra acquired in interleaved mode benefit from higher SNR (factor of 1.34± 0.06 for PCr) compared to standard acquisition due to the Nuclear Overhauser effect (NOE) and substantial PCr/Pi changes during exercise can be observed in 31P spectra. 1H spectral quality is equal to that in single mode experiments and allows Cr2 changes to be monitored. 4 Conclusion The feasibility of dynamic interleaved localised 1H and 31P spectroscopy during plantar flexion exercise has been demonstrated using a custom-built pneumatic system for muscle activation. This opens the possibility of studying the dynamics of metabolism with multi nuclear MRS in a single run. PMID:16320091

  1. Outcome-related metabolomic patterns from 1H/31P NMR after mild hypothermia treatments of oxygen–glucose deprivation in a neonatal brain slice model of asphyxia

    PubMed Central

    Liu, Jia; Litt, Lawrence; Segal, Mark R; Kelly, Mark J S; Yoshihara, Hikari A I; James, Thomas L

    2011-01-01

    Human clinical trials using 72 hours of mild hypothermia (32°C–34°C) after neonatal asphyxia have found substantially improved neurologic outcomes. As temperature changes differently modulate numerous metabolite fluxes and concentrations, we hypothesized that 1H/31P nuclear magnetic resonance (NMR) spectroscopy of intracellular metabolites can distinguish different insults, treatments, and recovery stages. Three groups of superfused neonatal rat brain slices underwent 45 minutes oxygen–glucose deprivation (OGD) and then were: treated for 3 hours with mild hypothermia (32°C) that began with OGD, or similarly treated with hypothermia after a 15-minute delay, or not treated (normothermic control group, 37°C). Hypothermia was followed by 3 hours of normothermic recovery. Slices collected at different predetermined times were processed, respectively, for 14.1 Tesla NMR analysis, enzyme-linked immunosorbent assay (ELISA) cell-death quantification, and superoxide production. Forty-nine NMR-observable metabolites underwent a multivariate analysis. Separated clustering in scores plots was found for treatment and outcome groups. Final ATP (adenosine triphosphate) levels, severely decreased at normothermia, were restored equally by immediate and delayed hypothermia. Cell death was decreased by immediate hypothermia, but was equally substantially greater with normothermia and delayed hypothermia. Potentially important biomarkers in the 1H spectra included PCr-1H (phosphocreatine in the 1H spectrum), ATP-1H (adenosine triphosphate in the 1H spectrum), and ADP-1H (adenosine diphosphate in the 1H spectrum). The findings suggest a potential role for metabolomic monitoring during therapeutic hypothermia. PMID:20717124

  2. Outcome-related metabolomic patterns from 1H/31P NMR after mild hypothermia treatments of oxygen-glucose deprivation in a neonatal brain slice model of asphyxia.

    PubMed

    Liu, Jia; Litt, Lawrence; Segal, Mark R; Kelly, Mark J S; Yoshihara, Hikari A I; James, Thomas L

    2011-02-01

    Human clinical trials using 72 hours of mild hypothermia (32°C-34°C) after neonatal asphyxia have found substantially improved neurologic outcomes. As temperature changes differently modulate numerous metabolite fluxes and concentrations, we hypothesized that (1)H/(31)P nuclear magnetic resonance (NMR) spectroscopy of intracellular metabolites can distinguish different insults, treatments, and recovery stages. Three groups of superfused neonatal rat brain slices underwent 45 minutes oxygen-glucose deprivation (OGD) and then were: treated for 3 hours with mild hypothermia (32°C) that began with OGD, or similarly treated with hypothermia after a 15-minute delay, or not treated (normothermic control group, 37°C). Hypothermia was followed by 3 hours of normothermic recovery. Slices collected at different predetermined times were processed, respectively, for 14.1 Tesla NMR analysis, enzyme-linked immunosorbent assay (ELISA) cell-death quantification, and superoxide production. Forty-nine NMR-observable metabolites underwent a multivariate analysis. Separated clustering in scores plots was found for treatment and outcome groups. Final ATP (adenosine triphosphate) levels, severely decreased at normothermia, were restored equally by immediate and delayed hypothermia. Cell death was decreased by immediate hypothermia, but was equally substantially greater with normothermia and delayed hypothermia. Potentially important biomarkers in the (1)H spectra included PCr-(1)H (phosphocreatine in the (1)H spectrum), ATP-(1)H (adenosine triphosphate in the (1)H spectrum), and ADP-(1)H (adenosine diphosphate in the (1)H spectrum). The findings suggest a potential role for metabolomic monitoring during therapeutic hypothermia. PMID:20717124

  3. Optically enhanced nuclear cross polarization in acridine-doped fluorene

    SciTech Connect

    Oshiro, C.M.

    1982-06-01

    The objective of this work has been to create large polarizations of the dilute /sup 13/C nuclei in the solid state. The idea was to create /sup 1/H polarizations larger than Boltzmann and to use the proton enhanced nuclear induction spectroscopy cross polarization technique to then transfer this large polarization to the /sup 13/C spin system. Optical Nuclear Polarization (ONP) of acridine-doped fluorene single crystals was studied. In addition, ONP of powdered samples of the acridine-doped fluorene was studied. In general, many compounds do not crystallize easily or do not form large crystals suitable for NMR experiments. Powdered, amorphous and randomly dispersed samples are generally far more readily available than single crystals. One objective of this work has been to (first) create large /sup 1/H polarizations. Although large optical proton polarizations in single crystals have been reported previously, optically generated polarizations in powdered samples have not been reported. For these reasons, ONP studies of powdered samples of the acridine-doped fluorene were also undertaken. Using ONP in combination with the proton enhanced nuclear induction spectroscopy experiment, large /sup 13/C polarizations have been created in fluorene single crystals. These large /sup 13/C polarizations have permitted the determination of the seven incongruent chemical shielding tensors of the fluorene molecule. Part 2 of this thesis describes the proton enhanced nuclear induction spectroscopy experiment. Part 3 describes the ONP experiment. Part 4 is a description of the experimental set-up. Part 5 describes the data analysis for the determination of the chemical shielding tensors. Part 6 presents the results of the ONP experiments performed in this work and the chemical shielding tensors determined.

  4. Cross polarization optical coherence tomography for diagnosis of oral soft tissues

    NASA Astrophysics Data System (ADS)

    Gladkova, Natalia; Karabut, Maria; Kiseleva, Elena; Robakidze, Natalia; Muraev, Alexander; Fomina, Julia

    2011-03-01

    We consider the capabilities of cross-polarization OCT (CP OCT) focused on comparison of images resulting from cross-polarization and co-polarization scattering simultaneously for diagnosis of oral soft tissues. CP OCT was done for 35 patients with dental implants and 30 patients with inflammatory intestine diseases. Our study showed good diagnostic capabilities of CP OCT for detecting soft tissue pathology in the oral cavity. The cross-polarized images demonstrate the ability of tissue to depolarize. CP OCT demonstrates clinical capabilities for early diagnosis of inflammatory intestine diseases by the state of oral cavity mucosa and for early detection of gingivitis in patients above implant.

  5. Ice Shelf Modeling: A Cross-Polar Bayesian Statistical Approach

    NASA Astrophysics Data System (ADS)

    Kirchner, N.; Furrer, R.; Jakobsson, M.; Zwally, H. J.

    2010-12-01

    Ice streams interlink glacial terrestrial and marine environments: embedded in a grounded inland ice such as the Antarctic Ice Sheet or the paleo ice sheets covering extensive parts of the Eurasian and Amerasian Arctic respectively, ice streams are major drainage agents facilitating the discharge of substantial portions of continental ice into the ocean. At their seaward side, ice streams can either extend onto the ocean as floating ice tongues (such as the Drygalsky Ice Tongue/East Antarctica), or feed large ice shelves (as is the case for e.g. the Siple Coast and the Ross Ice Shelf/West Antarctica). The flow behavior of ice streams has been recognized to be intimately linked with configurational changes in their attached ice shelves; in particular, ice shelf disintegration is associated with rapid ice stream retreat and increased mass discharge from the continental ice mass, contributing eventually to sea level rise. Investigations of ice stream retreat mechanism are however incomplete if based on terrestrial records only: rather, the dynamics of ice shelves (and, eventually, the impact of the ocean on the latter) must be accounted for. However, since floating ice shelves leave hardly any traces behind when melting, uncertainty regarding the spatio-temporal distribution and evolution of ice shelves in times prior to instrumented and recorded observation is high, calling thus for a statistical modeling approach. Complementing ongoing large-scale numerical modeling efforts (Pollard & DeConto, 2009), we model the configuration of ice shelves by using a Bayesian Hiearchial Modeling (BHM) approach. We adopt a cross-polar perspective accounting for the fact that currently, ice shelves exist mainly along the coastline of Antarctica (and are virtually non-existing in the Arctic), while Arctic Ocean ice shelves repeatedly impacted the Arctic ocean basin during former glacial periods. Modeled Arctic ocean ice shelf configurations are compared with geological spatial

  6. Cross-polarization level in radiation from a microstrip dipole antenna

    NASA Astrophysics Data System (ADS)

    Hoorfar, Ahmad; Gupta, Kuldip C.; Chang, David C.

    1988-09-01

    A description is given of the cross-polarization level in radiation from a small horizontal electric dipole (HED) on a flat grounded dielectric substrate. The study is directed toward the design of a very low cross-polarization level in a linear array of microstrip antenna elements. Field expressions for a copolarized and crosspolarized microstrip HED for any arbitrary dipole orientation and array direction are derived.

  7. Spin-locking and cross-polarization under magic-angle spinning of uniformly labeled solids.

    PubMed

    Hung, Ivan; Gan, Zhehong

    2015-07-01

    Spin-locking and cross-polarization under magic-angle spinning are investigated for uniformly (13)C and (15)N labeled solids. In particular, the interferences from chemical shift anisotropy, and (1)H heteronuclear and (13)C homonuclear dipolar couplings are identified. The physical origin of these interferences provides guidelines for selecting the best (13)C and (15)N polarization transfer rf fields. Optimal settings for both the zero- and double-quantum cross-polarization transfer mechanisms are recommended. PMID:25965280

  8. Spin-locking and cross-polarization under magic-angle spinning of uniformly labeled solids

    NASA Astrophysics Data System (ADS)

    Hung, Ivan; Gan, Zhehong

    2015-07-01

    Spin-locking and cross-polarization under magic-angle spinning are investigated for uniformly 13C and 15N labeled solids. In particular, the interferences from chemical shift anisotropy, and 1H heteronuclear and 13C homonuclear dipolar couplings are identified. The physical origin of these interferences provides guidelines for selecting the best 13C and 15N polarization transfer rf fields. Optimal settings for both the zero- and double-quantum cross-polarization transfer mechanisms are recommended.

  9. Dual-band cross polarization converter in bi-layered complementary chiral metamaterial

    NASA Astrophysics Data System (ADS)

    Ma, Xiaolong; Xiao, Zhongyin; Liu, Dejun

    2016-05-01

    In this article, a new chiral metamaterial with giant optical activity (90°) around 225 and 285 THz has been proposed which can be acted as a dual-band cross polarization converter (CPC). During the frequency range of proposed CPC, the maximum transmission coefficient and cross polarization conversion efficiency are up to 0.55 and 0.998, respectively. Importantly, the corresponding ellipticities are almost zero at 225 and 285 THz.

  10. Quantification of cross polarization with relaxation compensated reciprocity relation in NMR

    NASA Astrophysics Data System (ADS)

    Shu, Jie; Chen, Qun; Zhang, Shanmin

    2008-09-01

    The reciprocity relation in solid state NMR has been extended to include the effects of spin-lattice relaxation in the rotating frame. This method was successfully applied to the experiments of Hartmann-Hahn cross polarization, making the originally non-quantified NMR spectra quantitative. In addition, it provides detailed dynamics of cross polarization that is often obscured by spin-lattice relaxation in the rotating frame and by some other effects.

  11. High-contrast coherent population trapping based on crossed polarizers method.

    PubMed

    Yano, Yuichiro; Goka, Shigeyoshi

    2014-12-01

    A method based on crossed polarizers to observe high-contrast coherent population trapping (CPT) resonance has been developed. Because crossed polarizers have a simple optical system, our method is suitable for chip-scale atomic clocks (CSACs). In CPT, the Faraday rotation in a linearly polarized light field (lin||lin) was calculated using two pairs of Λ-system models; the spectrum of the Faraday rotation is also estimated. After measuring the contrast and linewidth with the crossed-polarizer method, a comparison of the theoretical model and experimental data showed they were in good agreement. Moreover, the experimental results showed that a high contrast (88.4%) and narrow linewidth (1.15 kHz) resonance could be observed using a Cs gas cell and D1-line verticalcavity surface-emitting laser (VCSEL). PMID:25474771

  12. Broadband cross polarization converter using plasmon hybridizations in a ring/disk cavity.

    PubMed

    Shi, Hongyu; Li, Jianxing; Zhang, Anxue; Wang, Jiafu; Xu, Zhuo

    2014-08-25

    In this paper, we report on the design, fabrication and subsequent investigation of a broad band cross polarization converter based on a C2-symmetric ring/disk cavity. Different plasmon hybridization modes are excited in the ring/disk cavity and enable the polarization manipulations. The designed cross polarization converter can convert the x or y polarized incident wave to its cross polarized wave in the frequency range from 9.65 to 14.16 GHz with a bandwidth of ~38% of the central wavelength and an efficiency higher than 80%. At 9.25 GHz and 14.35 GHz, the x (y) polarized incident wave is converted to a left (right) handed and right (left) handed circularly polarized wave, respectively. PMID:25321298

  13. A Low Cross-Polarization Smooth-Walled Horn with Improved Bandwidth

    NASA Technical Reports Server (NTRS)

    Zeng, Lingzhen; Bennett, Charles L.; Chuss, David T.; Wollack, Edward J.

    2009-01-01

    Corrugated feed horns offer excellent beam symmetry, main beam efficiency, and cross-polar response over wide bandwidths, but can be challenging to fabricate. An easier-to-manufacture smooth-walled feed is explored that approximates these properties over a finite bandwidth. The design, optimization and measurement of a monotonically-profiled, smooth-walled scalar feedhorn with a diffraction-limited approximately 7 degrees full width at half maximum (FWHM) is presented. The feed was demonstrated to have low cross polarization (<-30 dB) across the frequency range 33-45 GHz (30% fractional bandwidth). A return loss better than -28 dB was measured across the band.

  14. Cross polarization caused by perturbed circular cross sections of waveguides and horn antennas

    NASA Astrophysics Data System (ADS)

    Lier, Erik

    1987-03-01

    The cross polarization caused by a perturbed cross section of the conical hybrid-mode horn is analyzed. The perturbed cross section is assumed to be slightly elliptical. The theory of Lier and Bergh (1986) for cross polarization in a smooth-walled waveguide supporting the TE11-mode is referred and applied to the HE11 mode as well. Simple analytical formulas which are sufficiently accurate for small ellipticites of the cross-section ellipse are presented. These show that the tolerances on the waveguide diameter are extremely strong, typically on the order of 0.02-0.04 mm in the horn throat for typical horn geometries at 12 GHz.

  15. A Low Cross-Polarization Smooth-Walled Horn with Improved Bandwidth

    NASA Technical Reports Server (NTRS)

    Zeng, Lingzhen; Bennette, Charles L.; Chuss, David T.; Wollack, Edward J.

    2009-01-01

    Corrugated feed horns offer excellent beam symmetry, main beam efficiency, and cross-polar response over wide bandwidths, but can be challenging to fabricate. An easier-to-manufacture smooth-walled feed is explored that approximates these properties over a finite bandwidth. The design, optimization and measurement of a monotonically-profiled, smooth-walled scalar feedhorn with a diffraction-limited approx. 14deg FWHM beam is presented. The feed was demonstrated to have low cross polarization (<-30 dB) across the frequency range 33-45 GHz (30% fractional bandwidth). A power reflection below -28 dB was measured across the band.

  16. Cross-polarized wave generation by effective cubic nonlinear optical interaction.

    PubMed

    Petrov, G I; Albert, O; Etchepare, J; Saltiel, S M

    2001-03-15

    A new cubic nonlinear optical effect in which a linearly polarized wave propagating in a single quadratic medium is converted into a wave that is cross polarized to the input wave is observed in BBO crystal. The effect is explained by cascading of two different second-order processes: second-harmonic generation and difference frequency mixing. PMID:18040322

  17. On the cross-polarization characteristics of crooked wire antennas designed by genetic-algorithms

    NASA Technical Reports Server (NTRS)

    Rengarajan, S. R.; Rahmat-Samii, Y.

    2002-01-01

    In many modern communication applications there is a need for simple circularly polarized antennas for hemispherical coverage with good axial ratio or low value of cross polarization. We revisited the crooked wire antenna because of its simplicity. This paper presents results of our investigation on the crooked wire antennas and other elements.

  18. Simultaneous cross polarization to 13C and 15N with 1H detection at 60 kHz MAS solid-state NMR

    NASA Astrophysics Data System (ADS)

    Das, Bibhuti B.; Opella, Stanley J.

    2016-01-01

    We describe high resolution MAS solid-state NMR experiments that utilize 1H detection with 60 kHz magic angle spinning; simultaneous cross-polarization from 1H to 15N and 13C nuclei; bidirectional cross-polarization between 13C and 15N nuclei; detection of both amide nitrogen and aliphatic carbon 1H; and measurement of both 13C and 15N chemical shifts through multi-dimensional correlation experiments. Three-dimensional experiments correlate amide 1H and alpha 1H selectively with 13C or 15N nuclei in a polypeptide chain. Two separate three-dimensional spectra correlating 1Hα/13Cα/1HN and 1HN/15N/1Hα are recorded simultaneously in a single experiment, demonstrating that a twofold savings in experimental time is potentially achievable. Spectral editing using bidirectional coherence transfer pathways enables simultaneous magnetization transfers between 15N, 13Cα(i) and 13C‧(i-1), facilitating intra- and inter-residue correlations for sequential resonance assignment. Non-uniform sampling is integrated into the experiments, further reducing the length of experimental time.

  19. Circularly Polarized Microwave Antenna Element with Very Low Off-Axis Cross-Polarization

    NASA Technical Reports Server (NTRS)

    Greem. David; DuToit, Cornelis

    2013-01-01

    The goal of this work was to improve off-axis cross-polarization performance and ease of assembly of a circularly polarized microwave antenna element. To ease assembly, the initial design requirement of Hexweb support for the internal circuit part, as well as the radiating disks, was eliminated. There is a need for different plating techniques to improve soldering. It was also desirable to change the design to eliminate soldering as well as the need to use the Hexweb support. Thus, a technique was developed to build the feed without using solder, solving the lathing and soldering issue. Internal parts were strengthened by adding curvature to eliminate Hexweb support, and in the process, the new geometries of the internal parts opened the way for improving the off-axis cross-polarization performance as well. The radiating disks curvatures were increased for increased strength, but it was found that this also improved crosspolarization. Optimization of the curvatures leads to very low off-axis cross-polarization. The feed circuit was curved into a cylinder for improved strength, eliminating Hexweb support. An aperture coupling feed mechanism eliminated the need for feed pins to the disks, which would have required soldering. The aperture coupling technique also improves cross-polarization performance by effectively exciting the radiating disks very close to the antenna s central axis of symmetry. Because of the shape of the parts, it allowed for an all-aluminum design bolted together and assembled with no solder needed. The advantage of a solderless design is that the reliability is higher, with no single-point failure (solder), and no need for special plating techniques in order to solder the unit together. The shapes (curved or round) make for a more robust build without extra support materials, as well as improved offaxis cross-polarization.

  20. Tri-band transparent cross-polarization converters using a chiral metasurface

    NASA Astrophysics Data System (ADS)

    Shi, Hong-Yu; Li, Jian-Xing; Zhang, An-Xue; Wang, Jia-Fu; Xu, Zhuo

    2014-11-01

    A chiral metasurface is proposed to realize a tri-band polarization angle insensitive cross-polarization converter. The unit cell of the chiral metamaterial is composed by four twisted anisotropic structure pairs in four-fold rotation symmetry. The simulation results show that this device can work at 9.824 GHz, 11.39 GHz, and 13.37 GHz with low loss and a high polarization conversion ratio (PCR) of more than 99%. The proposed design can transmit the co-polarization wave at 14.215 GHz, like a frequency selective surface. The study of the current and electric fields distributions indicates that the cross-polarization transmission is due to electric dipole coupling.

  1. Wideband tunable mid-infrared cross polarization converter using rectangle-shape perforated graphene.

    PubMed

    Yang, Chen; Luo, Yi; Guo, Jiaxin; Pu, Yang; He, De; Jiang, Yadong; Xu, Jimmy; Liu, Zhijun

    2016-07-25

    The strong plasmonic response and wide electrostatic tunability of graphene make it a promising material for developing infrared optoelectronic components. In this paper, we present a mid-infrared wideband tunable cross polarization converter using periodically perforated graphene. The polarization converter consists of a metal ground plane, an insulator layer, and a rectangle-shape periodically perforated graphene sheet. By superimposing two localized surface plasmon modes, the polarization converter transforms a linear polarization to its cross polarization over a bandwidth as wide as ~5% of the central frequency (46.8THz) with a peak conversion ratio exceeding 90%. The polarization conversion performance is maintained over a wide range of incident angles up to 50°, and is highly tunable by electrostatic tuning of the graphene Fermi energy. Our proposed device enables the manipulation of light polarization for potential mid-infrared applications. PMID:27464143

  2. Ergodicity and efficiency of cross-polarization in NMR of static solids

    NASA Astrophysics Data System (ADS)

    Nevzorov, Alexander A.

    2011-04-01

    Cross-polarization transfer is employed in virtually every solid-state NMR experiment to enhance magnetization of low-gamma spins. Theory and experiment is used to assess the magnitude of the final quasistationary magnetization amplitude. The many-body density matrix equation is solved for relatively large (up to N = 14) spin systems without the spin-temperature assumption for the final spin states. Simulations show that about 13% of the thermodynamic limit is still retained within the proton bath. To test this theoretical prediction, a combination of a reverse cross-polarization experiment and multiple contacts is employed to show that the thermodynamic limit of magnetization cannot be transferred from high- to low-gamma nuclei in a single contact. Multiple contacts, however, fully transfer the maximum magnetization. A simple diffusion on a cone model shows that slow dynamics can affect the build up profile for the transferred magnetization.

  3. Ergodicity and efficiency of cross-polarization in NMR of static solids.

    PubMed

    Nevzorov, Alexander A

    2011-04-01

    Cross-polarization transfer is employed in virtually every solid-state NMR experiment to enhance magnetization of low-gamma spins. Theory and experiment is used to assess the magnitude of the final quasistationary magnetization amplitude. The many-body density matrix equation is solved for relatively large (up to N=14) spin systems without the spin-temperature assumption for the final spin states. Simulations show that about 13% of the thermodynamic limit is still retained within the proton bath. To test this theoretical prediction, a combination of a reverse cross-polarization experiment and multiple contacts is employed to show that the thermodynamic limit of magnetization cannot be transferred from high- to low-gamma nuclei in a single contact. Multiple contacts, however, fully transfer the maximum magnetization. A simple diffusion on a cone model shows that slow dynamics can affect the build up profile for the transferred magnetization. PMID:21296016

  4. Surface configuration as an explanation for lithology-related cross-polarized radar image anomalies

    NASA Technical Reports Server (NTRS)

    Mccauley, J. R.

    1973-01-01

    One problem that has persisted since the development of multipolarized radar is the cause or causes of differential depolarization which is expressed as tonal reversals between like- and cross-polarized images of certain outcrops. Rocks producing anomalously low returns on the cross-polarized image could be classed into three general types: (1) certain geologically recent lava flows (late Pleistocene and Holocene), (2) some tertiary volcanics and (3) certain massive sandstones. Differential depolarization has been produced by volcanic rocks of various compositions including rhyolite, rhyodacite, dacite, andesite, and basalt. This has led to the conclusion that differential depolarization is not directly caused by any compositional factor. However, the study of aerial photos and subsequent field observation have led to the conclusion that the weathering and other surface characteristics of the outcrops are responsible for their appearance on multipolarized imagery.

  5. Unidirectional cross polarization rotator with enhanced broadband transparency by cascading twisted nanobars

    NASA Astrophysics Data System (ADS)

    Wang, Ying-Hua; Shao, Jian; Li, Jie; Zhu, Ming-Jie; Li, Jiaqi; Dong, Zheng-Gao

    2016-05-01

    We demonstrate the optical activity for linear polarization by twisting cascading multilayer nanobars, for which the x- (y-)polarized light is significantly transformed to a y- (x-)polarized one with enhanced transmittance in a unidirectional manner, and the bandwidth can be broadened by increasing the cascading number of layers. The polarization conversion rate reaches nearly 100% with a maximum cross-polarization transmission coefficient larger than 0.95. This phenomenon is attributed to the chiral structural arrangement and anisotropic resonance of nanobars, which consequently leads to different cross-polarization conversions between forward and backward incident lights, and thus the unidirectional transmission with an extinction ratio up to 103. These characteristics show application potential in optical nano-devices.

  6. Tailored low-power cross-polarization under fast magic-angle spinning

    NASA Astrophysics Data System (ADS)

    Demers, Jean-Philippe; Vijayan, Vinesh; Becker, Stefan; Lange, Adam

    2010-08-01

    High static magnetic fields and very fast magic-angle spinning (MAS) promise to improve resolution and sensitivity of solid-state NMR experiments. The fast MAS regime has permitted the development of low-power cross-polarization schemes, such as second-order cross-polarization (SOCP), which prevent heat deposition in the sample. Those schemes are however limited in bandwidth, as weak radio-frequency (RF) fields only cover a small chemical shift range for rare nuclei (e.g. 13C). Another consideration is that the efficiency of cross-polarization is very sensitive to magnetization decay that occurs during the spin-lock pulse on the abundant nuclei (e.g. 1H). Having characterized this decay in glutamine at 60 kHz MAS, we propose two complementary strategies to tailor cross-polarization to desired spectral regions at low RF power. In the case of multiple sites with small chemical shift dispersion, a larger bandwidth for SOCP is obtained by slightly increasing the RF power while avoiding recoupling conditions that lead to fast spin-lock decay. In the case of two spectral regions with large chemical shift offset, an extension of the existing low-power schemes, called MOD-CP, is introduced. It consists of a spin-lock on 1H and an amplitude-modulated spin-lock on the rare nucleus. The range of excited chemical shifts is assessed by experimental excitation profiles and numerical simulation of an I 2S spin system. All SOCP-based schemes exhibit higher sensitivity than high-power CP schemes, as demonstrated on solid (glutamine) and semi-solid (hydrated, micro-crystalline ubiquitin) samples.

  7. Optical cross-polarization converter with an octave bandwidth based on anisotropic plasmonic meta-surfaces

    NASA Astrophysics Data System (ADS)

    Zhang, Liang; Song, Zhengyong; Huo Liu, Qing

    2015-07-01

    We show that the linear polarization state of electromagnetic waves can be efficiently rotated to its orthogonal direction by an alternative design of anisotropic plasmonic meta-surfaces. Numerical results demonstrate that the reflection coefficient converting to cross-polarization after reflection is larger than 71% within an octave frequency band from ∼232 \\text{THz} to ∼490 \\text{THz} . The designed system is much thinner than conventional geometric-optics devices, and takes favorable advantage of little energy loss.

  8. Nonlinear spectral cleaning of few-cycle pulses via cross-polarized wave (XPW) generation

    NASA Astrophysics Data System (ADS)

    Jullien, A.; Durfee, C. G.; Trisorio, A.; Canova, L.; Rousseau, J.-P.; Mercier, B.; Antonucci, L.; Chériaux, G.; Albert, O.; Lopez-Martens, R.

    2009-08-01

    The characterization of a temporal filter based on cross-polarized wave generation working in the few-optical-cycle regime is presented. We show that this device dramatically improves the spectral quality of the ultrashort seed pulses, opening the way to the production of sub-10 fs pulses with high incoherent and coherent contrast. The dispersion compensation conditions for an optimized behavior of the process are experimentally and theoretically discussed.

  9. DESIGN AND PERFORMANCE OF A LOW-FREQUENCY CROSS-POLARIZED LOG-PERIODIC DIPOLE ANTENNA

    SciTech Connect

    Raja, K. Sasikumar; Kathiravan, C.; Ramesh, R.; Rajalingam, M.; Barve, Indrajit V.

    2013-07-01

    We report the design and performance of a cross-polarized log-periodic dipole (CLPD) antenna for observations of polarized radio emission from the solar corona at low frequencies. The measured isolation between the two mutually orthogonal log-periodic dipole antennas was as low as Almost-Equal-To - 43 dBm in the 65-95 MHz range. We carried out observations of the solar corona at 80 MHz with the above CLPD and successfully recorded circularly polarized emission.

  10. CTS attenuation and cross-polarization measurements at 11.7 GHz

    NASA Technical Reports Server (NTRS)

    Vogel, W. J.; Straiton, A. W.

    1977-01-01

    The results of data obtained 80 days preceding the solar eclipse shutdown of the CTS 11.7 GHz righthand circularly polarized beacon transmitter are presented. Attenuation and cross polarization isolation were measured. It was determined that depolarization presents a serious limitation to satellite system reliability when frequency reuse by polarization diversity is employed. A 27 db isolation margin would reduce reliability below 99.95%. For the same percentage the required fade margin was below 3 db.

  11. Variable-effective-field cross polarization. An approach to broadband Hartmann-Hahn matching in MAS NMR

    NASA Astrophysics Data System (ADS)

    Kolbert, Andrew C.; Gann, Sheryl L.

    1994-07-01

    A method for broadening the Hartmann-Hahn matching condition for cross polarization under magic angle spinning is presented. Variable-effective-field cross polarization (VEFCP) employs different effective raido-frequency (rf) fields on the 1H channel during mixing, while the 13C rf field remains constant. VEFCP is shown to result in both a broader matching condition, and a more rapid polarization transfer than ordinary cross polarization at the Hartmann-Hahn match. Experimental results on both adamantane and polycarbonate are discussed.

  12. Hybridizing cross-polarization with NOE or refocused-INEPT enhances the sensitivity of MAS NMR spectroscopy.

    PubMed

    Zhang, Rongchun; Mroue, Kamal H; Ramamoorthy, Ayyalusamy

    2016-05-01

    Heteronuclear cross polarization (CP) has been commonly used to enhance the sensitivity of dilute low-γ nuclei in almost all solid-state NMR experiments. However, CP relies on heteronuclear dipolar couplings, and therefore the magnetization transfer efficiency becomes inefficient when the dipolar couplings are weak, as is often the case for mobile components in solids. Here, we demonstrate methods that combine CP with heteronuclear Overhauser effect (referred to as CP-NOE) or with refocused-INEPT (referred to as CP-RINEPT) to overcome the efficiency limitation of CP and enhance the signal-to-noise ratio (S/N) for mobile components. Our experimental results reveal that, compared to the conventional CP, significant S/N ratio enhancement can be achieved for resonances originating from mobile components, whereas the resonance signals associated with rigid groups are not significantly affected due to their long spin-lattice relaxation times. In fact, the S/N enhancement factor is also dependent on the temperature, CP contact time as well as on the system under investigation. Furthermore, we also demonstrate that CP-RINEPT experiment can be successfully employed to independently detect mobile and rigid signals in a single experiment without affecting the data collection time. However, the resolution of CP spectrum obtained from the CP-RINEPT experiment could be slightly compromised by the mandatory use of continuous wave (CW) decoupling during the acquisition of signals from rigid components. In addition, CP-RINEPT experiment can be used for spectral editing utilizing the difference in dynamics of different regions of a molecule and/or different components present in the sample, and could also be useful for the assignment of resonances from mobile components in poorly resolved spectra. Therefore, we believe that the proposed approaches are beneficial for the structural characterization of multiphase and heterogeneous systems, and could be used as a building block in

  13. Hybridizing cross-polarization with NOE or refocused-INEPT enhances the sensitivity of MAS NMR spectroscopy

    NASA Astrophysics Data System (ADS)

    Zhang, Rongchun; Mroue, Kamal H.; Ramamoorthy, Ayyalusamy

    2016-05-01

    Heteronuclear cross polarization (CP) has been commonly used to enhance the sensitivity of dilute low-γ nuclei in almost all solid-state NMR experiments. However, CP relies on heteronuclear dipolar couplings, and therefore the magnetization transfer efficiency becomes inefficient when the dipolar couplings are weak, as is often the case for mobile components in solids. Here, we demonstrate methods that combine CP with heteronuclear Overhauser effect (referred to as CP-NOE) or with refocused-INEPT (referred to as CP-RINEPT) to overcome the efficiency limitation of CP and enhance the signal-to-noise ratio (S/N) for mobile components. Our experimental results reveal that, compared to the conventional CP, significant S/N ratio enhancement can be achieved for resonances originating from mobile components, whereas the resonance signals associated with rigid groups are not significantly affected due to their long spin-lattice relaxation times. In fact, the S/N enhancement factor is also dependent on the temperature, CP contact time as well as on the system under investigation. Furthermore, we also demonstrate that CP-RINEPT experiment can be successfully employed to independently detect mobile and rigid signals in a single experiment without affecting the data collection time. However, the resolution of CP spectrum obtained from the CP-RINEPT experiment could be slightly compromised by the mandatory use of continuous wave (CW) decoupling during the acquisition of signals from rigid components. In addition, CP-RINEPT experiment can be used for spectral editing utilizing the difference in dynamics of different regions of a molecule and/or different components present in the sample, and could also be useful for the assignment of resonances from mobile components in poorly resolved spectra. Therefore, we believe that the proposed approaches are beneficial for the structural characterization of multiphase and heterogeneous systems, and could be used as a building block in

  14. Efficient cross-polarization using a composite 0° pulse for NMR studies on static solids

    NASA Astrophysics Data System (ADS)

    Fukuchi, Masashi; Ramamoorthy, Ayyalusamy; Takegoshi, K.

    2009-02-01

    In most solid-state NMR experiments, cross-polarization is an essential step to detect low-γ nuclei such as 13C and 15N. In this study, we present a new cross-polarization scheme using spin-locks composed of composite 0° pulses in the RF channels of high-γ and low-γ nuclei to establish the Hartmann-Hahn match. The composite 0° pulses with no net nutation-angle{(2 π) X - (2 π) -X - (2 π) Y - (2 π) -Y -} n applied simultaneously to both high-γ ( I) and low-γ ( S) nuclei create an effective heteronuclear dipolar Hamiltonian Hd(0)={d}/{2}(2IZSZ+IXSX+IYSY), which is capable of transferring the Z-component of the I spin magnetization to the Z-component of the S spin magnetization. It also retains a homonuclear dipolar coupling Hamiltonian that enables the flip-flop transfer among abundant spins. While our experimental results indicate that the new pulse sequence, called composite zero cross- polarization (COMPOZER-CP) performs well on adamantane, it is expected to be more valuable to study semi-solids like liquid crystalline materials and model lipid membranes. Theoretical analysis of COMPOZER-CP is presented along with experimental results. Our experimental results demonstrate that COMPOZER-CP overcomes the RF field inhomogeneity and Hartmann-Hahn mismatch for static solids. Experimental results comparing the performance of COMPOZER-CP with that of the traditional constant-amplitude CP and rampCP sequences are also presented in this paper.

  15. Single Shot Polarization Characterization of XUV FEL Pulses from Crossed Polarized Undulators

    PubMed Central

    Ferrari, E.; Allaria, E.; Buck, J.; De Ninno, G.; Diviacco, B.; Gauthier, D.; Giannessi, L.; Glaser, L.; Huang, Z.; Ilchen, M.; Lambert, G.; Lutman, A. A.; Mahieu, B.; Penco, G.; Spezzani, C.; Viefhaus, J.

    2015-01-01

    Polarization control is a key feature of light generated by short-wavelength free-electron lasers. In this work, we report the first experimental characterization of the polarization properties of an extreme ultraviolet high gain free-electron laser operated with crossed polarized undulators. We investigate the average degree of polarization and the shot-to-shot stability and we analyze aspects such as existing possibilities for controlling and switching the polarization state of the emitted light. The results are in agreement with predictions based on Gaussian beams propagation. PMID:26314764

  16. Characterization of atherosclerotic plaques by cross-polarization optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Gubarkova, Ekaterina V.; Dudenkova, Varvara V.; Feldchtein, Felix I.; Timofeeva, Lidia B.; Kiseleva, Elena B.; Kuznetsov, Sergei S.; Moiseev, Alexander A.; Gelikonov, Gregory V.; Vitkin, Alex I.; Gladkova, Natalia D.

    2016-02-01

    We combined cross-polarization optical coherence tomography (CP OCT) and non-linear microscopy based on second harmonic generation (SHG) and two-photon-excited fluorescence (2PEF) to assess collagen and elastin fibers in the development of the atherosclerotic plaque (AP). The study shows potential of CP OCT for the assessment of collagen and elastin fibers condition in atherosclerotic arteries. Specifically, the additional information afforded by CP OCT, related to birefringence and cross-scattering properties of arterial tissues, may improve the robustness and accuracy of assessment about the microstructure and composition of the plaque for different stages of atherosclerosis.

  17. Single shot polarization characterization of XUV FEL pulses from crossed polarized undulators

    DOE PAGESBeta

    Ferrari, E.; Allaria, E.; Buck, J.; De Ninno, G.; Diviacco, B.; Gauthier, D.; Giannessi, L.; Glaser, L.; Huang, Z.; Ilchen, M.; et al

    2015-08-28

    Polarization control is a key feature of light generated by short-wavelength free-electron lasers. In this work, we report the first experimental characterization of the polarization properties of an extreme ultraviolet high gain free-electron laser operated with crossed polarized undulators. We research the average degree of polarization and the shot-to-shot stability and we analyze aspects such as existing possibilities for controlling and switching the polarization state of the emitted light. The results are in agreement with predictions based on Gaussian beams propagation.

  18. Observation of solid-state 103Rh NMR by cross-polarization.

    PubMed

    Phillips, Brian L; Houston, Jacqueline R; Feng, Jian; Casey, William H

    2006-03-29

    Using 103Rh[1H] cross-polarization (CP) methods, we have obtained solid-state 103Rh NMR spectra for diamagnetic Rh(III) compounds. The isotropic chemical shift and chemical shift anisotropy (CSA) are reported for a crystalline form of the dihydroxy-bridged Rh(III) dimer and for a salt of the oxo-centered acetate-bridged Rh(III) trimer, from analysis of conventional CP/MAS spectra. Comparison of the CP kinetics of the dimer with new crystal structure data suggests a strategy for predicting 103Rh CP/MAS properties in solids. PMID:16551090

  19. Low-temperature cross polarization in view of enhancing dissolution Dynamic Nuclear Polarization in NMR

    NASA Astrophysics Data System (ADS)

    Jannin, Sami; Bornet, Aurélien; Colombo, Sonia; Bodenhausen, Geoffrey

    2011-12-01

    Dynamic Nuclear Polarization (DNP) induced by saturation of ESR transitions of TEMPO at 1.2 K and 3.35 T is characterized by build-up rates that are typically 5 times faster for protons than for the carboxylic carbon-13 in acetate. We show that cross polarization from protons to carbon-13 allows one to achieve a polarization P( 13C) >20% in less than 10 min, twice as much as has been previously reported, in one-fifth of the time. This should open the way to an unprecedented improvement in the efficiency of dissolution DNP.

  20. Impact of cross-polarization isolation on polarimetric target decomposition and target detection

    NASA Astrophysics Data System (ADS)

    Xu, Feng; Wang, Haipeng; Jin, Ya-Qiu; Liu, Xiuqing; Wang, Robert; Deng, Yunkai

    2015-04-01

    Cross-polarization isolation is one of the key engineering parameters for a polarimetric radar system. Previous studies focused more on the calibration of cross-talk contamination. This paper presents a numerical evaluation of the requirement for cross-polarization isolation from the data users' perspective, i.e., the quantitative impact of polarization cross talk on polarimetric target decomposition and the associated applications such as classification and detection. Sensitivity analyses of several commonly used target decomposition parameters suggest that a theoretical lower bound of -32 dB isolation level is preferred to avoid any significant impact on these parameters. Our analyses with both simulated and real synthetic aperture radar (SAR) data show that a level of -25 dB would be acceptable for general terrain surface classification. This requirement is also true for man-made target detection application. Using simulated SAR images of man-made targets in natural environment, sensitivity analyses on two polarimetric detectors, Yang and Marino, both suggest that target detection performance would break down rapidly if isolation deteriorates from -25 dB to -20 dB.

  1. Influence of dental resin material composition on cross-polarization-optical coherence tomography imaging

    PubMed Central

    Lammeier, Carmen; Li, YuPing; Lunos, Scott; Fok, Alex; Rudney, Joel

    2012-01-01

    Abstract. The purpose of this study was to investigate cross-polarization-optical coherence tomography (CP-OCT) signal attenuation through different resin material compositions. Four distinct composite systems were used: Filtek supreme ultra (FSU) (3M ESPE), IPS empress direct (EMD) (Ivoclar Vivadent), estelite sigma quick (SQK) (Tokuyama Dental), and Z100 (3M ESPE). Cross-sectional images of different composite-demineralized phantoms (n=108) were collected using a 1310-nm intraoral cross-polarization swept source OCT (CP-OCT) imaging system. %T quantified the CP-OCT signal attenuation. Scanning electron microscopy, transmission electron microscopy, and energy-dispersive x-ray spectrometer chemical analysis was utilized to determine how different matrix/filler compositions affected attenuation of the near infrared (NIR) signal. CP-OCT imaging of dental resin composites showed enormous variation in signal attenuation. For each of our composite systems, there was not a consistent attenuation difference in the NIR signal for A to D shades. The four composites had similar measured backscattering values but attenuated the overall signal to different degrees. When comparing the A2 shades between the four different composite systems, the order of highest to lowest of %T was EMD>Z100, FSU>SQK (ANOVA, Tukey, p<0.0001). As a result, we demonstrate the importance of understanding how the constituents of composite materials affect CP-OCT signal attenuation. PMID:23224001

  2. Monitoring tooth demineralization using a cross polarization optical coherence tomographic system with an integrated MEMS scanner

    NASA Astrophysics Data System (ADS)

    Fried, Daniel; Staninec, Michal; Darling, Cynthia; Kang, Hobin; Chan, Kenneth

    2012-01-01

    New methods are needed for the nondestructive measurement of tooth demineralization and remineralization to monitor the progression of incipient caries lesions (tooth decay) for effective nonsurgical intervention and to evaluate the performance of anti-caries treatments such as chemical treatments or laser irradiation. Studies have shown that optical coherence tomography (OCT) has great potential to fulfill this role since it can be used to measure the depth and severity of early lesions with an axial resolution exceeding 10-μm, it is easy to apply in vivo and it can be used to image the convoluted topography of tooth occlusal surfaces. In this paper we present early results using a new cross-polarization OCT system introduced by Santec. This system utilizes a swept laser source and a MEMS scanner for rapid acquisition of cross polarization images. Preliminary studies show that this system is useful for measurement of the severity of demineralization on tooth surfaces and for showing the spread of occlusal lesions under the dentinal-enamel junction.

  3. Evaluation of cross-polarized near infrared hyperspectral imaging for early detection of dental caries

    NASA Astrophysics Data System (ADS)

    Usenik, Peter; Bürmen, Miran; Fidler, Aleš; Pernuš, Franjo; Likar, Boštjan

    2012-01-01

    Despite major improvements in dental healthcare and oral hygiene, dental caries remains one of the most prevalent oral diseases and represents the primary cause of oral pain and tooth loss. The initial stages of dental caries are characterized by demineralization of enamel crystals and are difficult to diagnose. Near infrared (NIR) hyperspectral imaging is a new promising technique for detection of early changes in the surfaces of carious teeth. This noninvasive imaging technique can characterize and differentiate between the sound tooth surface and initial or advanced tooth caries. The absorbing and scattering properties of dental tissues reflect in distinct spectral features, which can be measured, quantified and used to accurately classify and map different dental tissues. Specular reflections from the tooth surface, which appear as bright spots, mostly located around the edges and the crests of the teeth, act as a noise factor which can significantly interfere with the spectral measurements and analysis of the acquired images, degrading the accuracy of the classification and diagnosis. Employing cross-polarized imaging setup can solve this problem, however has yet to be systematically evaluated, especially in broadband hyperspectral imaging setups. In this paper, we employ cross-polarized illumination setup utilizing state-of-the-art high-contrast broadband wire-grid polarizers in the spectral range from 900 nm to 1700 nm for hyperspectral imaging of natural and artificial carious lesions of various degrees.

  4. Nondestructive monitoring of the repair of natural occlusal lesions using cross polarization optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Kang, Hobin; Darling, Cynthia L.; Fried, Daniel

    2012-01-01

    Previous remineralization studies employing cross polarization sensitive optical coherence tomography (CP-OCT), have been limited to the repair of artificial enamel-like lesions. In this study we attempted to remineralize existing occlusal lesions on extracted teeth. Lesions were imaged before and after exposure to an acidic remineralization regimen and the integrated reflectivity and lesion depth was calculated. Automated integration routines worked well for assessing the integrated reflectivity for the lesion areas after remineralization. Polarized light microscopy was also used to examine the lesions areas after sectioning the teeth. An acidic remineralization solution was used to remineralize the lesions. The integrated reflectivity significantly increased after exposure to the remineralization solution which suggests that the acidic solution caused additional demineralization as opposed to the desired remineralization.

  5. Square-wave switching by crossed-polarization gain modulation in vertical-cavity semiconductor lasers

    SciTech Connect

    Mulet, J.; Giudici, M.; Javaloyes, J.; Balle, S.

    2007-10-15

    We study experimentally and theoretically the effects of crossed-polarization reinjection (XPR) on the output characteristics of a vertical-cavity semiconductor laser. We find a set of parameters values for which each polarization component develops a square-wave modulation at a period close to twice the reinjection delay. We analyze the regularity of this modulation in terms of the laser pumping current and of the reinjection level. These observations are numerically reproduced within the spin-flip model modified to account for XPR. In particular, the degradation of the square-wave switching is linked to the finite value of the spin-flip rate, and it occurs when the current approaches the boundaries of polarization bistability.

  6. Improving temporal contrast by cross-polarized wave generation at a sub-petawatt laser facility

    NASA Astrophysics Data System (ADS)

    Xie, Na; Wang, Xiaodong; Huang, Wanqing; Sun, Li; Guo, Yi; Li, Qing; Zhao, Runchang; Su, Jingqin

    2011-10-01

    Temporal contrast is an important factor affecting the application of ultraintense and ultrashort laser systems. In this paper, we employ cross-polarized wave (XPW) generation to improve the temporal contrast for ultraintense and ultrashort pulses in a 300 TW Ti:Sapphire laser facility, i.e. the super intense laser for experiment on the extremes (SILEX-I). We designed a double chirped-pulse amplification (CPA) system with an intermediate nonlinear temporal pulse filter based on XPW generation and the estimated output energy is more than 300 mJ for the new front-end system. The experimental results show that the output energy of the double CPA system is greater than 370 mJ. The amplified spontaneous emission (ASE) pedestal is suppressed significantly and the temporal contrast is improved by more than two orders of magnitude.

  7. The hat feed - A dual-mode rear-radiating waveguide antenna having low cross polarization

    NASA Astrophysics Data System (ADS)

    Kildal, Per-Simon

    1987-09-01

    The hat antenna is a new self-supported rear-radiating waveguide feed, related to existing feeds known as splashplate and waveguide cup antennas. An analytical model for the radiation pattern of the hat antenna, a model which includes the axial waveguide itself is presented. This model shows that by exciting the feed with two modes it is possible to use the waveguide constructively as one of the dominant radiating parts of the feed instead of having to live with it as an undesirable blockage effect. Thereby aperture efficiencies up to 81 percent and cross-polar sidelobes down to -30 dB are available. The results are confirmed by measurements on a practical model, but the bandwidth is narrow.

  8. Efficient cross polarized wave generation for compact, energy-scalable, ultrashort laser sources.

    PubMed

    Ramirez, L P; Papadopoulos, D N; Pellegrina, A; Georges, P; Druon, F; Monot, P; Ricci, A; Jullien, A; Chen, X; Rousseau, J P; Lopez-Martens, R

    2011-01-01

    The generation of high contrast and ultrashort laser pulses via a compact and energy-scalable cross polarized wave filter is presented. The setup incorporates a waveguide spatial filter into a single crystal XPW configuration, enabling high energy and high intensity transmission, efficient contrast enhancement and pulse shortening at the multi-mJ level. Excellent XPW conversion of up to 33% (global efficiency: 20%, intensity transmission: 40%) led to an output energy of 650 µJ for an input of 3.3 mJ. Additionally, efficient conversion under specific input phase conditions, allowed pulse shortening from 25 fs to 9.6 fs, indicating the prospective application of this setup as a high energy, ultrabroad laser source. PMID:21263545

  9. Low-cost label-free biosensors using photonic crystals embedded between crossed polarizers.

    PubMed

    Nazirizadeh, Yousef; Bog, Uwe; Sekula, Sylwia; Mappes, Timo; Lemmer, Uli; Gerken, Martina

    2010-08-30

    There is a strong need for low-cost biosensors to enable rapid, on-site analysis of biological, biomedical, or chemical substances. We propose a platform for label-free optical biosensors based on applying the analyte onto a surface-functionalized photonic crystal slab and performing a transmission measurement with two crossed polarization filters. This dark-field approach allows for efficient background suppression as only the photonic crystal guided-mode resonances interacting with the functionalized surface experience significant polarization rotation. We present a compact biosensor demonstrator using a low-cost light emitting diode and a simple photodiode capable of detecting the binding kinetics of a 2.5 nM solution of the protein streptavidin on a biotin-functionalized photonic crystal surface. PMID:20940807

  10. Remineralization of root caries monitored using cross polarization optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Darling, Cynthia L.; Staninec, Michal; Chan, Kenneth H.; Kang, Hobin; Fried, Daniel

    2012-01-01

    Previous studies have demonstrated that polarization sensitive optical coherence tomography (PS-OCT) can be used to image caries lesions in dentin, measure nondestructively the severity of dentin demineralization, and determine the efficacy of intervention with anticaries agents including fluoride and lasers. However, those studies were limited to artificial lesions on dentin and roots surfaces. The objective of this study is to determine if a cross polarization OCT system (CP-OCT) can be used to nondestructively measure a reduction in the reflectivity of natural root caries lesions after exposure to a remineralization solution. CPOCT images of 11 teeth with existing root lesions were acquired before and after exposure to a remineralizing solution for 20 days. The integrated reflectivity was calculated after integrating to a fixed depth of 200-μm. There was a significant decrease in the integrated reflectivity after exposure to the remineralizing solution.

  11. Ray Scattering by an Arbitrarily Oriented Spheroid: 2. Transmission and Cross-polarization Effects

    NASA Technical Reports Server (NTRS)

    Lock, James A.

    1996-01-01

    Transmission of an arbitrarily polarized plane wave by an arbitrarily oriented spheroid in the short-wavelength limit is considered in the context of ray theory. The transmitted electric field is added to the diffracted plus reflected ray-theory electric field that was previously derived to obtain an approximation to the far-zone scattered intensity in the forward hemisphere. Two different types of cross-polarization effects are found. These are: (a) a rotation of the polarization state of the transmitted rays from when they are referenced with respect to their entrance into the spheroid to when they are referenced with respect to their exit from it and (b) a rotation of the polarization state of the transmitted rays when they are referenced with respect to the polarization state of the diffracted plus reflected rays.

  12. Quantitative solid-state 13C NMR with signal enhancement by multiple cross polarization

    NASA Astrophysics Data System (ADS)

    Johnson, Robert L.; Schmidt-Rohr, Klaus

    2014-02-01

    A simple new method is presented that yields quantitative solid-state magic-angle spinning (MAS) 13C NMR spectra of organic materials with good signal-to-noise ratios. It achieves long (>10 ms) cross polarization (CP) from 1H without significant magnetization losses due to relaxation and with a moderate duty cycle of the radio-frequency irradiation, by multiple 1-ms CP periods alternating with 1H spin-lattice relaxation periods that repolarize the protons. The new method incorporates previous techniques that yield less distorted CP/MAS spectra, such as a linear variation (“ramp”) of the radio-frequency field strength, and it overcomes their main limitation, which is T1ρ relaxation of the spin-locked 1H magnetization. The ramp of the radio-frequency field strength and the asymptotic limit of cross polarization makes the spectral intensity quite insensitive to the exact field strengths used. The new multiCP pulse sequence is a “drop-in” replacement for previous CP methods and produces no additional data-processing burden. Compared to the only reliable quantitative 13C NMR method for unlabeled solids previously available, namely direct-polarization NMR, the measuring time is reduced by more than a factor of 50, enabling higher-throughput quantitative NMR studies. The new multiCP technique is validated with 14-kHz MAS on amino-acid derivatives, plant matter, a highly aromatic humic acid, and carbon materials made by low-temperature pyrolysis.

  13. Cross-Polar Aircraft Trajectory Optimization and the Potential Climate Impact

    NASA Technical Reports Server (NTRS)

    Ng, Hok K.; Sridhar, Banavar; Grabbe, Shon; Chen, Neil

    2011-01-01

    Cross-Polar routes offer new opportunities for air travel markets. Transpolar flights reduce travel times, fuel burns, and associated environmental emissions by flying direct paths between many North American and Asian cities. This study evaluates the potential benefits of flying wind-optimal polar routes and assessed their potential impact on climate change. An optimization algorithm is developed for transpolar flights to generate wind-optimal trajectories that minimize climate impact of aircraft, in terms of global warming potentials (relative to warming by one kg of CO2) of several types of emissions, while avoiding regions of airspace that facilitate persistent contrail formation. Estimations of global warming potential are incorporated into the objective function of the optimization algorithm to assess the climate impact of aircraft emissions discharged at a given location and altitude. The regions of airspace with very low ambient temperature and areas favorable to persistent contrail formation are modeled as undesirable regions that aircraft should avoid and are formulated as soft state constraints. The fuel burn and climate impact of cross-polar air traffic flying various types of trajectory including flight plan, great circle, wind-optimal, and contrail-avoidance are computed for 15 origin-destination pairs between major international airports in the U.S. and Asia. Wind-optimal routes reduce average fuel burn of flight plan routes by 4.4% on December 4, 2010 and 8.0% on August 7, 2010, respectively. The tradeoff between persistent contrail formation and additional global warming potential of aircraft emissions is investigated with and without altitude optimization. Without altitude optimization, the reduction in contrail travel times is gradual with increase in total fuel consumption. When altitude is optimized, a one percent increase in additional global warming potential, a climate impact equivalent to that of 4070kg and 4220kg CO2 emission, reduces 135

  14. A cross-polarized freeform illumination design for glare reduction in fruit quality inspection

    NASA Astrophysics Data System (ADS)

    Keresztes, Janos C.; Koshel, R. John; Chipman, Russel; Stover, John C.; Saeys, Wouter

    2015-09-01

    Common illumination systems in short wave infrared (SWIR) hyperspectral imaging (HSI) include direct or indirect tungsten halogen lights. While direct lights provide more radiation onto the samples than dome setups, thus being more energy efficient, the acquired images often suffer from specular reflections and gloss. Glare artifacts in images increase variability in the data limiting the accuracy of machine vision algorithms for defect detection and quality inspection, or even providing false positives. Although domes are known to provide a near Lambertian illumination and glare free images, glossy regions and heterogeneities may remain in the data in practice. More particularly, in the field of fruit and vegetable quality inspection, due to their waxy surface, it remains challenging to design an efficient realistic lighting system. This paper suggests a new approach to optimize the illumination of fruit and vegetables based on measurements of the bidirectional reflectance distribution function (BRDF), shape and Stokes parameters. From these measured values, a BRDF model is loaded into ray-tracing software for realistic illumination engineering in order to determine the most suitable illumination scheme. This concept is applied to apples and a cross polarizer (CP) with freeform optics (FO) optical configuration is proposed, which allows the FO to be optimized to maximize uniformity in the field of view of the imager and removes the parallel polarized gloss on the apples. The performance of this CP illumination system was determined experimentally for a set of apples. This cross polarized (CP) illumination system provided a uniformity (U) of 92% and an efficiency (ν) of 90%, while U = 87% and ν = 14% for an ideal dome configuration when illuminating a rectangular target. The simulated imaged apples with assigned optical properties performed better with CP (U=80%) than when using a dome (U=73%) by 7%. Finally, the sensitivity of the design for the light

  15. 13C and 31P chemical shielding tensors of a single crystal of dipotassium α- D-glucose-1-phosphate dihydrate. An application of a 13C-{ 1H, 31P} triple-resonance technique

    NASA Astrophysics Data System (ADS)

    McDowell, C. A.; Naito, A.; Sastry, D. L.; Takegoshi, K.

    The 13C NMR spectra of a single crystal of dipotassium α- D-glucose-l-phosphate dehydrate for different orientations in the external magnetic field, were recorded by using 1H and 31P double nuclear decoupling. To overcome difficulties encountered because of the high 13C RF power required to achieve the Hartmann-Hahn condition, a new cross-polarization method (K. Takegoshi and C. A. McDowell, J. Magn. Reson.67, 356 (1986)) was used. The directions of the most shielded principal value of the 13C chemical shielding tensors for the C2-C6 carbon nuclei in the glucose group were along the CO bond, and that for the CI carbon nucleus made an angle of 42† with the C1-O5 bond direction in the O1-C1-O5 plane. The 31P chemical shielding tensors are axially symmetric and the direction of the least shielded principal value is almost parallel to the P-O1(R) bond, which is the longest among the four PO bonds in the phosphate moiety.

  16. Realization of broadband cross-polarization conversion in transmission mode in the terahertz region using a single-layer metasurface.

    PubMed

    Liu, Wenwei; Chen, Shuqi; Li, Zhancheng; Cheng, Hua; Yu, Ping; Li, Jianxiong; Tian, Jianguo

    2015-07-01

    We present the design specifications and in-depth analysis of a terahertz (THz) broadband cross-polarization converter composed of a single-layer metasurface. This device can convert linearly polarized light into its cross-polarization in transmission mode. Different from other polarization conversion devices, this effect results from the suppression and enhancement for different electric components. The broadband characteristic is also achieved by specific partial symmetries designed in the structure. The proposed polarization converter can aid in the development of novel plasmonic polarization devices, and can help to overcome certain limitations of the customary designs that have been proposed thus far. PMID:26125398

  17. Dynamics of cross polarization in solid state nuclear magnetic resonance experiments of amorphous and heterogeneous natural organic substances.

    PubMed

    Conte, Pellegrino; Berns, Anne E

    2008-09-01

    Nuclear magnetic resonance (NMR) experiments on carbon-13 in the solid state were done with cross polarization (CP) and magic angle spinning (MAS) in order to overcome the low NMR sensitivity of (13)C and the chemical shift anisotropy, respectively. In the present research, CPMAS (13)C-NMR spectra were collected by modulating the contact time needed for cross polarization (variable contact times experiments, VCT) on two different humic acids (a soil-HA and a coal-HA). VCT data were fitted by a model containing either a monotonic or a non-monotonic cross polarization term. The non-monotonic model, which fitted the experimental results better than the monotonic one, provided two cross-polarization rates, thereby suggesting that two different mechanisms for the energy transfer from protons to carbons arise in amorphous and heterogeneous humic substances. The first mechanism was a fast proton-to-carbon energy transfer due to protons directly bound to carbons. The second mechanism was related to a slow transfer mediated by local segmental motions. Different domains in the humic acids were identified. Soil-HA was made of rigid domains, containing mainly aromatic and carboxylic moieties, and fast moving domains, containing alkyl, C-O and C-O groups. Coal-HA showed a rigid aromatic domain that was differentiated from a very mobile domain made of alkyl and COOH groups. PMID:18781033

  18. Parallel and series FED microstrip array with high efficiency and low cross polarization

    NASA Technical Reports Server (NTRS)

    Huang, John (Inventor)

    1995-01-01

    A microstrip array antenna for vertically polarized fan beam (approximately 2 deg x 50 deg) for C-band SAR applications with a physical area of 1.7 m by 0.17 m comprises two rows of patch elements and employs a parallel feed to left- and right-half sections of the rows. Each section is divided into two segments that are fed in parallel with the elements in each segment fed in series through matched transmission lines for high efficiency. The inboard section has half the number of patch elements of the outboard section, and the outboard sections, which have tapered distribution with identical transmission line sections, terminated with half wavelength long open-circuit stubs so that the remaining energy is reflected and radiated in phase. The elements of the two inboard segments of the two left- and right-half sections are provided with tapered transmission lines from element to element for uniform power distribution over the central third of the entire array antenna. The two rows of array elements are excited at opposite patch feed locations with opposite (180 deg difference) phases for reduced cross-polarization.

  19. Cross-polarization phenomena in the NMR of fast spinning solids subject to adiabatic sweeps

    SciTech Connect

    Wi, Sungsool E-mail: lucio.frydman@weizmann.ac.il; Gan, Zhehong; Schurko, Robert; Frydman, Lucio E-mail: lucio.frydman@weizmann.ac.il

    2015-02-14

    Cross-polarization magic-angle spinning (CPMAS) experiments employing frequency-swept pulses are explored within the context of obtaining broadband signal enhancements for rare spin S = 1/2 nuclei at very high magnetic fields. These experiments employ adiabatic inversion pulses on the S-channel ({sup 13}C) to cover a wide frequency offset range, while simultaneously applying conventional spin-locking pulse on the I-channel ({sup 1}H). Conditions are explored where the adiabatic frequency sweep width, Δν, is changed from selectively irradiating a single magic-angle-spinning (MAS) spinning centerband or sideband, to sweeping over multiple sidebands. A number of new physical features emerge upon assessing the swept-CP method under these conditions, including multiple zero- and double-quantum CP transfers happening in unison with MAS-driven rotary resonance phenomena. These were examined using an average Hamiltonian theory specifically designed to tackle these experiments, with extensive numerical simulations, and with experiments on model compounds. Ultrawide CP profiles spanning frequency ranges of nearly 6⋅γB{sub 1}{sup s} were predicted and observed utilizing this new approach. Potential extensions and applications of this extremely broadband transfer conditions are briefly discussed.

  20. Clinical cross-polarization optical coherence tomography assessment of subsurface enamel below dental resin composite restorations

    PubMed Central

    Lenton, Patricia; Rudney, Joel; Fok, Alex; Jones, Robert S.

    2014-01-01

    Abstract. A newly designed intraoral swept source cross-polarization optical coherence tomography (CP-OCT) imaging system was used to examine the integrity of the subsurface enamel below resin composite restorations placed in primary teeth. CP-OCT analysis was performed using images obtained from resin composite restoration in 62 (n=62) pediatric subjects. Clinical examination was performed by a single examiner prior to CP-OCT imaging and analysis. CP-OCT images are presented using a unique combined intensity image, where a false color scale is overlaid on the grayscale intensity image. There was a clear difference in the distribution of the mean-backscattered intensity (mR) between restorations recently placed and those possessing frank cavitation (Student’s t-test, P<0.0001). For mR above 15.49 dB, the sensitivity was 80% and specificity 86%. The Youden index J was 0.8 above 12.3 dB where sensitivity was 100% and specificity was 80%. CP-OCT imaging may be used to confirm the subsurface marginal integrity below resin composite restorations but with careful consideration of limitations of the imaging modality. CP-OCT imaging may be a useful adjunct to clinical visual investigation to confirm that a composite margin has a sound and well-adapted interface. PMID:26158031

  1. Cross-polarization phenomena in the NMR of fast spinning solids subject to adiabatic sweeps.

    PubMed

    Wi, Sungsool; Gan, Zhehong; Schurko, Robert; Frydman, Lucio

    2015-02-14

    Cross-polarization magic-angle spinning (CPMAS) experiments employing frequency-swept pulses are explored within the context of obtaining broadband signal enhancements for rare spin S = 1/2 nuclei at very high magnetic fields. These experiments employ adiabatic inversion pulses on the S-channel ((13)C) to cover a wide frequency offset range, while simultaneously applying conventional spin-locking pulse on the I-channel ((1)H). Conditions are explored where the adiabatic frequency sweep width, Δν, is changed from selectively irradiating a single magic-angle-spinning (MAS) spinning centerband or sideband, to sweeping over multiple sidebands. A number of new physical features emerge upon assessing the swept-CP method under these conditions, including multiple zero- and double-quantum CP transfers happening in unison with MAS-driven rotary resonance phenomena. These were examined using an average Hamiltonian theory specifically designed to tackle these experiments, with extensive numerical simulations, and with experiments on model compounds. Ultrawide CP profiles spanning frequency ranges of nearly 6⋅γB1 (s) were predicted and observed utilizing this new approach. Potential extensions and applications of this extremely broadband transfer conditions are briefly discussed. PMID:25681899

  2. Nonlinear dynamics of double-pass cross-polarized wave generation in the saturation regime.

    PubMed

    Iliev, Marin; Meier, Amanda K; Greco, Michael; Durfee, Charles G

    2015-01-10

    The conversion efficiency of cross-polarized wave (XPW) generation can be improved using two separate thinner nonlinear crystals versus a single thick one, due to the evolution of the beam sizes and individual phases after the first crystal. In this paper, we present an alternative scheme in which a curved mirror is used to reimage a plane just after the BaF2 crystal for a second pass. We also develop a simple analytic model for XPW conversion that describes the origin of a nonlinear phase mismatch and nonlinear lensing for both the fundamental wave and XPW. Coupled with the numerical solution for the process and the Fresnel propagation after the first pass, we also explore the factors that affect the efficiency of saturated, seeded XPW conversion. These include the development of the on-axis relative phase difference in the first crystal and after it (during free-space propagation), mode matching, wavefront curvature difference, and crystal tuning angle. We also experimentally demonstrate that the beam quality of the XPW signal after the second pass can be improved by the reimaging. PMID:25967620

  3. Broadband adiabatic inversion pulses for cross polarization in wideline solid-state NMR spectroscopy

    NASA Astrophysics Data System (ADS)

    Harris, Kristopher J.; Lupulescu, Adonis; Lucier, Bryan E. G.; Frydman, Lucio; Schurko, Robert W.

    2012-11-01

    Efficient acquisition of ultra-wideline solid-state NMR powder patterns is a continuing challenge. In particular, when the breadth of the powder pattern is much larger than the cross-polarization (CP) excitation bandwidth, transfer efficiencies suffer and experimental times are greatly increased. Presented herein is a CP pulse sequence with an excitation bandwidth that is up to ten times greater than that available from a conventional spin-locked CP pulse sequence. The pulse sequence, broadband adiabatic inversion CP (BRAIN-CP), makes use of the broad, uniformly large frequency profiles of chirped inversion pulses, to provide these same characteristics to the polarization transfer process. A detailed theoretical analysis is given, providing insight into the polarization transfer process involved in BRAIN-CP. Experiments on spin-1/2 nuclei including 119Sn, 199Hg and 195Pt nuclei are presented, and the large bandwidth improvements possible with BRAIN-CP are demonstrated. Furthermore, it is shown that BRAIN-CP can be combined with broadband frequency-swept versions of the Carr-Purcell-Meiboom-Gill experiment (for instance with WURST-CPMG, or WCPMG for brevity); the combined BRAIN-CP/WCPMG experiment then provides multiplicative signal enhancements of both CP and multiple-echo acquisition over a broad frequency region.

  4. Clinical cross-polarization optical coherence tomography assessment of subsurface enamel below dental resin composite restorations.

    PubMed

    Lenton, Patricia; Rudney, Joel; Fok, Alex; Jones, Robert S

    2014-04-01

    A newly designed intraoral swept source cross-polarization optical coherence tomography (CP-OCT) imaging system was used to examine the integrity of the subsurface enamel below resin composite restorations placed in primary teeth. CP-OCT analysis was performed using images obtained from resin composite restoration in 62 ([Formula: see text]) pediatric subjects. Clinical examination was performed by a single examiner prior to CP-OCT imaging and analysis. CP-OCT images are presented using a unique combined intensity image, where a false color scale is overlaid on the grayscale intensity image. There was a clear difference in the distribution of the mean-backscattered intensity (mR) between restorations recently placed and those possessing frank cavitation (Student's t-test, [Formula: see text]). For mR above 15.49 dB, the sensitivity was 80% and specificity 86%. The Youden index J was 0.8 above 12.3 dB where sensitivity was 100% and specificity was 80%. CP-OCT imaging may be used to confirm the subsurface marginal integrity below resin composite restorations but with careful consideration of limitations of the imaging modality. CP-OCT imaging may be a useful adjunct to clinical visual investigation to confirm that a composite margin has a sound and well-adapted interface. PMID:26158031

  5. Longitudinal monitoring of demineralization peripheral to orthodontic brackets using cross polarization optical coherence tomography

    PubMed Central

    Nee, Alexander; Chan, Kenneth; Kang, Hobin; Staninec, Michal; Darling, Cynthia L.; Fried, Daniel

    2014-01-01

    Objectives The aim of this study was to test the hypothesis that cross-polarization optical coherence tomography (CP-OCT) can be used to longitudinally monitor demineralization peripheral to orthodontic brackets in an extended clinical study. Methods A high-speed CP-OCT system was used to acquire 3D volumetric images of the area at the base of orthodontic brackets over a period of 12-months after placement. The reflectivity was measured at 3-month intervals for 12-months to determine if there was increased demineralization. Two teeth were monitored on twenty test subjects and the brackets were bonded using two types of adhesives This was a randomized controlled clinical study with a split mouth design such that each subject served as his or her own control. On one side, the control premolar was bonded with a bonding agent (Adper Scotchbond from 3M ESPE, St. Paul, MN) and composite (Transbond XT from 3M Unitek, Monrovia, CA) that lacked fluoride. On the other side, the experimental premolar was bonded with a fluoride releasing glass ionomer cement (GC Fuji Ortho LC from GC America, Alsip, IL). Results There was a small but significant increase in the calculated lesion depth and integrated reflectivity over that depth (Δ R) for both adhesive types (p<0.0001) indicating increasing demineralization with time. There was no significant difference in the lesion depth (p=0.22) and Δ R (p=0.91) between the groups with the fluoride releasing glass ionomer cement and the conventional composite. Conclusions CP-OCT was able to measure a significant increase in demineralization (P<0.0001) at the base of orthodontic brackets over a period of 12-months. PMID:24561340

  6. Heteronuclear cross-polarization in multinuclear multidimensional NMR: Prospects for triple-resonance CP

    SciTech Connect

    Majumdar, A.; Zuiderweg, E.R.P.

    1994-12-01

    Heteronuclear multiple-pulse-based Cross Polarization (HECP) between scalar coupled spins is gaining an important role in high-resolution multidimensional NMR of isotopically labeled biomolecules, especially in experiments involving net magnetization transfer. It has generally been observed that in these situations, the performance of HECP is superior to that of INEPT-based sequences. In particular, HECP-based three-dimensional HCCH spectroscopy is more efficient than the INEPT version of the same experiment. Differences in sensitivity have been intuitively attributed to relaxation effects and technical factors such as radiofrequency (rf) inhomogeneity We present theoretical analyses and computer simulations to probe the effects of these factors. Relaxation effects were treated phenomenologically; we found that relaxation differences are relatively small (up to 25%) between pulsed-free-precession (INEPT) and HECP-although always in favor of HECP. We explored the rf effects by employing a Gaussian distribution of rf amplitude over sample volume. We found that inhomogeneity effects significantly favor HECP over INEPT, especially under conditions of {open_quotes}matched {close_quotes} inhomogeneity in the two rf coils. The differences in favor of HECP indicate that an extension of HECP to triple resonance experiments (TRCP) in I -> S -> Q net transfers might yield better results relative to analogous INEPT-based net transfers. We theoretically analyze the possibilities of TRCP and find that transfer functions are critically dependent on the ratio J{sub IS}/J{sub SQ}. When J{sub IS} equals J{sub SQ}, we find that 100% transfer is possible for truly simultaneous TRCP and this transfer is obtained in a time 1.41 /J. The TRCP time requirement compares favorably with optimally concatenated INEPT-transfers, where net transfer I -> S -> Q is complete at 1.5 /J.

  7. Improved Cross Polarization and Broad Impedance Bandwidth from Simple Single Element Shorted Rectangular Microstrip Patch: Theory and Experiment

    NASA Astrophysics Data System (ADS)

    Poddar, Rakshapada; Chakraborty, Subhradeep; Chattopadhyay, Sudipta

    2016-01-01

    A simple, compact and single element rectangular microstrip antenna with three pairs of shorting plates has been proposed and investigated experimentally for broad impedance bandwidth and improved cross polarized (XP) radiation compared to maximum co-polarized (CO) gain without affecting the co-polarized radiation pattern. Around 25-40 dB isolation between copolarized radiation to cross polarized radiation (CO-XP isolation) along with 1.32 GHz impedance bandwidth is achieved with the proposed structure. The present structure is very simple and easy to manufacture and provides high CO-XP isolation over entire angular range around the broadside direction. Moreover, the present structure is free from back radiation in terms of XP fields. The present investigation provides an insightful, visualization-based understanding of concurrent improvement in impedance bandwidth and the XP radiation characteristics with the present structure.

  8. Phase dynamics in vertical-cavity surface-emitting lasers with delayed optical feedback and cross-polarized reinjection

    NASA Astrophysics Data System (ADS)

    Javaloyes, J.; Marconi, M.; Giudici, M.

    2014-08-01

    We study theoretically the nonlinear polarization dynamics of vertical-cavity surface-emitting lasers in the presence of an external cavity providing delayed optical feedback and cross-polarized reinjection. We show that, far from the laser threshold, the dynamics remains confined close to the equatorial plane of a Poincaré sphere with a fixed radius. It entails that the evolution of the system is described by two phase variables: the orientation phase of the quasilinear polarization and the optical phase of the field. We explore the complex modal structure given by the double reinjection configuration and how it evolves between the cases of single cross-polarized reinjection and single optical feedback, hence disclosing the relationship with the Lang-Kobayashi model. We also reinterpret the square-wave switching observed by J. Mulet et al. [Phys. Rev. A 76, 043801 (2007), 10.1103/PhysRevA.76.043801] in terms of phase kinks.

  9. Proton observed phosphorus editing (POPE) for in vivo detection of phospholipid metabolites.

    PubMed

    Wijnen, Jannie P; Klomp, Dennis W J; Nabuurs, Christine I H C; de Graaf, Robin A; van Kalleveen, Irene M L; van der Kemp, Wybe J M; Luijten, Peter R; Kruit, Mark C; Webb, Andrew; Kan, Hermien E; Boer, Vincent O

    2016-09-01

    The purpose of this article was to compare the sensitivity of proton observed phosphorus editing (POPE) with direct (31) P MRS with Ernst angle excitation for (1) H-(31) P coupled metabolites at 7 T. POPE sequences were developed for detecting phosphocholine (PC), phosphoethanolamine (PE), glycerophosphocholine (GPC), and glycerophosphoethanolamine (GPE) on the (1) H channel, thereby using the enhanced sensitivity of the (1) H nuclei over (31) P detection. Five healthy volunteers were examined with POPE and (31) P-MRS. POPE editing showed a more than doubled sensitivity in an ideal phantom experiment as compared with direct (31) P MRS with Ernst angle excitation. In vivo, despite increased relaxation losses, significant gains in signal-to-noise ratio (SNR) of 30-40% were shown for PE and GPE + PC levels in the human brain. The SNR of GPC was lower in the POPE measurement compared with the (31) P-MRS measurement. Furthermore, selective narrowband editing on the (31) P channel showed the ability to separate the overlapping GPE and PE signals in the (1) H spectrum. POPE can be used for enhanced detection of (1) H-(31) P coupled metabolites in vivo. Copyright © 2015 John Wiley & Sons, Ltd. PMID:26601921

  10. Background-free imaging of plasmonic structures with cross-polarized apertureless scanning near-field optical microscopy.

    PubMed

    Esslinger, M; Dorfmüller, J; Khunsin, W; Vogelgesang, R; Kern, K

    2012-03-01

    We present advances in experimental techniques of apertureless scanning near-field optical microscopy (aSNOM). The rational alignment procedure we outline is based upon a phase singularity that occurs while scanning polarizers around the nominal cross-polarized configuration of s-polarized excitation and p-polarized detection. We discuss the theoretical origin of this topological feature of the setup, which is robust against small deviations, such as minor tip misalignment or shape variations. Setting the polarizers to this singular configuration point eliminates all background signal, allowing for reproducible plasmonic eigenmode mapping with optimal signal-to-noise ratio. PMID:22462926

  11. Measurement of energy contrast of amplified ultrashort pulses using cross-polarized wave generation and spectral interferometry.

    PubMed

    Iliev, Marin; Meier, Amanda K; Galloway, Benjamin; Adams, Daniel E; Squier, Jeff A; Durfee, Charles G

    2014-07-28

    We present a method using spectral interferometry (SI) to characterize a pulse in the presence of an incoherent background such as amplified spontaneous emission (ASE). The output of a regenerative amplifier is interfered with a copy of the pulse that has been converted using third-order cross-polarized wave generation (XPW). The ASE shows as a pedestal background in the interference pattern. The energy contrast between the short-pulse component and the ASE is retrieved. The spectra of the interacting beams are obtained through an improvement to the self-referenced spectral interferometry (SRSI) analysis. PMID:25089416

  12. Wide-Field-of-View Millimeter-Wave Telescope Design with Ultra-Low Cross-Polarization

    SciTech Connect

    Bernacki, Bruce E.; Kelly, James F.; Sheen, David M.; Hatchell, Brian K.; Valdez, Patrick LJ; Tedeschi, Jonathan R.; Hall, Thomas E.; McMakin, Douglas L.

    2012-05-01

    As millimeter-wave arrays become available, off-axis imaging performance of the fore optics increases in importance due to the relatively large physical extent of the arrays. Typically, simple optical telescope designs are adapted to millimeter-wave imaging but single-mirror spherical or classic conic designs cannot deliver adequate image quality except near the optical axis. Since most millimeter-wave designs are quasi-optical, optical ray tracing and commercial design software can be used to optimize designs to improve off-axis imaging as well as minimize cross-polarization. Methods that obey the Dragone-Mizuguchi condition for the design of reflective millimeter-wave telescopes with low cross-polarization also provide additional degrees of freedom that offer larger fields of view than possible with single-reflector designs. Dragone’s graphical design method does not lend itself readily to computer-based optical design approaches, but subsequent authors expanded on Dragone’s geometric design approach with analytic expressions that describe the location, shape, off-axis height and tilt of the telescope elements that satisfy Dragone’s design rules and can be used as a first-order design for subsequent computer-based design and optimization. We investigate two design variants that obey the Dragone-Mizuguchi conditions that exhibit ultra-low polarization crosstalk and a large diffraction-limited field of view well suited to millimeter-wave imaging arrays.

  13. Assessing ex vivo dental biofilms and in vivo composite restorations using cross-polarization optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Jones, R.; Aparicio, C.; Chityala, R.; Chen, R.; Fok, A.; Rudney, J.

    2012-01-01

    A cross-polarization 1310-nm optical coherence tomography system (CP-OCT), using a beam splitter based design, was used to assess ex vivo growth of complex multi-species dental biofilms. These biofilm microcosms were derived from plaque samples along the interface of composite or amalgam restoration in children with a history of early childhood caries. This paper presents a method of measuring the mean biofilm height of mature biofilms using CP-OCT. For our in vivo application, the novel swept source based CP-OCT intraoral probe (Santec Co. Komaki, Japan) dimensions and system image acquisition speed (20 image frames/second) allowed imaging pediatric subjects as young as 4 years old. The subsurface enamel under the interface of composite resin restorations of pediatric subjects were imaged using CP-OCT. Cavitated secondary caries is clearly evident from sound resin composite restorations.

  14. A Cross-Polarization Based Rotating-Frame Separated-Local-Field NMR Experiment Under Ultrafast MAS Conditions

    PubMed Central

    Zhang, Rongchun; Damron, Joshua; Vosegaard, Thomas

    2014-01-01

    Rotating-frame separated-local-field solid-state NMR experiments measure highly resolved heteronuclear dipolar couplings which, in turn, provide valuable interatomic distances for structural and dynamic studies of molecules in the solid-state. Though many different rotating-frame SLF sequences have been put forth, recent gains in ultrafast MAS technology have considerably simplified pulse sequence requirements due to the suppression of proton-proton dipolar interactions. In this study we revisit a simple two-dimensional 1H-13C dipolar coupling/chemical shift correlation experiment using 13C detected Cross-Polarization with a Variable Contact time (CPVC) and systematically study the conditions for its optimal performance at 60 kHz MAS. In addition, we demonstrate the feasibility of a proton-detected version of the CPVC experiment. The theoretical analysis of the CPVC pulse sequence under different Hartmann-Hahn matching conditions confirms that it performs optimally under the ZQ (w1H-w1C=±wr) condition for polarization transfer. The limits of the cross polarization process are explored and precisely defined as a function of offset and Hartmann-Hahn mismatch via spin dynamics simulation and experiments on a powder sample of uniformly 13C-labeled L-isoleucine. Our results show that the performance of the CPVC sequence and subsequent determination of 1H-13C dipolar couplings are insensitive to 1H/13C frequency offset frequency when high RF fields are used on both RF channels. Conversely, the CPVC sequence is quite sensitive to the Hartmann-Hahn mismatch, particularly for systems with weak heteronuclear dipolar couplings. We demonstrate the use of the CPVC based SLF experiment as a tool to identify different carbon groups, and hope to motivate the exploration of more sophisticated 1H detected avenues for ultrafast MAS. PMID:25486635

  15. Scattering of a cross-polarized linear wave by a soliton at an optical event horizon in a birefringent nanophotonic waveguide

    NASA Astrophysics Data System (ADS)

    Ciret, Charles; Gorza, Simon-Pierre

    2016-06-01

    The scattering of a linear wave on an optical event horizon, induced by a cross polarized soliton, is experimentally and numerically investigated in integrated structures. The experiments are performed in a dispersion-engineered birefringent silicon nanophotonic waveguide. In stark contrast with co-polarized waves, the large difference between the group velocity of the two cross-polarized waves enables a frequency conversion almost independent on the soliton wavelength. It is shown that the generated idler is only shifted by 10 nm around 1550 nm over a pump tuning range of 350 nm. Simulations using two coupled full vectorial nonlinear Schr\\"odinger equations fully support the experimental results.

  16. Scattering of a cross-polarized linear wave by a soliton at an optical event horizon in a birefringent nanophotonic waveguide.

    PubMed

    Ciret, Charles; Gorza, Simon-Pierre

    2016-06-15

    The scattering of a linear wave on an optical event horizon, induced by a cross-polarized soliton, is experimentally and numerically investigated in integrated structures. The experiments are performed in a dispersion-engineered birefringent silicon nanophotonic waveguide. In stark contrast with copolarized waves, the large difference between the group velocity of the two cross-polarized waves enables a frequency conversion almost independent of the soliton wavelength. It is shown that the generated idler is only shifted by 10 nm around 1550 nm over a pump tuning range of 350 nm. Simulations using two coupled full vectorial nonlinear Schrödinger equations fully support the experimental results. PMID:27304314

  17. The Technique of Film Editing. Enlarged Edition.

    ERIC Educational Resources Information Center

    Reisz, Karel; Millar, Gavin

    Film editing is discussed from the point of view, not only of the person in the cutting room, but also of the person who has responsibility for the final film. Part I outlines the history of editing from the silent film to 1953. It discusses the practice of editing for action, dialogue, comedy, and montage sequences, as well as in documentaries,…

  18. Modular video endoscopy for in vivo cross-polarized and vital-dye fluorescence imaging of Barrett's-associated neoplasia

    NASA Astrophysics Data System (ADS)

    Thekkek, Nadhi; Pierce, Mark C.; Lee, Michelle H.; Polydorides, Alexandros D.; Flores, Raja M.; Anandasabapathy, Sharmila; Richards-Kortum, Rebecca R.

    2013-02-01

    A modular video endoscope is developed and tested to allow imaging in different modalities. This system incorporates white light imaging (WLI), cross-polarized imaging (CPI), and vital-dye fluorescence imaging (VFI), using interchangeable filter modules. CPI and VFI are novel endoscopic modalities that probe mucosal features associated with Barrett's neoplasia. CPI enhances vasculature, while VFI enhances glandular architecture. In this pilot study, we demonstrate the integration of these modalities by imaging areas of Barrett's metaplasia and neoplasia in an esophagectomy specimen. We verify that those key image features are also observed during an in vivo surveillance procedure. CPI images demonstrate improved visualization of branching blood vessels associated with neoplasia. VFI images show glandular architecture with increased glandular effacement associated with neoplasia. Results suggests that important pathologic features seen in CPI and VFI are not visible during standard endoscopic white light imaging, and thus the modalities may be useful in future in vivo studies for discriminating neoplasia from Barrett's metaplasia. We further demonstrate that the integrated WLI/CPI/VFI endoscope is compatible with complementary high-resolution endomicroscopy techniques such as the high-resolution microendoscope, potentially enabling two-step ("red-flag" widefield plus confirmatory high-resolution imaging) protocols to be enhanced.

  19. Broadband cross-polarization-based heteronuclear dipolar recoupling for structural and dynamic NMR studies of rigid and soft solids.

    PubMed

    Kharkov, B B; Chizhik, V I; Dvinskikh, S V

    2016-01-21

    Dipolar recoupling is an essential part of current solid-state NMR methodology for probing atomic-resolution structure and dynamics in solids and soft matter. Recently described magic-echo amplitude- and phase-modulated cross-polarization heteronuclear recoupling strategy aims at efficient and robust recoupling in the entire range of coupling constants both in rigid and highly dynamic molecules. In the present study, the properties of this recoupling technique are investigated by theoretical analysis, spin-dynamics simulation, and experimentally. The resonance conditions and the efficiency of suppressing the rf field errors are examined and compared to those for other recoupling sequences based on similar principles. The experimental data obtained in a variety of rigid and soft solids illustrate the scope of the method and corroborate the results of analytical and numerical calculations. The technique benefits from the dipolar resolution over a wider range of coupling constants compared to that in other state-of-the-art methods and thus is advantageous in studies of complex solids with a broad range of dynamic processes and molecular mobility degrees. PMID:26801025

  20. Quantitative (13)C Solid-State NMR Spectra by Multiple-Contact Cross-polarization for Drug Delivery: From Active Principles to Excipients and Drug Carriers.

    PubMed

    Saïdi, Fadila; Taulelle, Francis; Martineau, Charlotte

    2016-08-01

    In this contribution, we present an analysis of the main parameters influencing the efficiency of the (1)H → (13)C multiple-contact cross-polarization nuclear magnetic resonance (NMR) experiment in the context of solid pharmaceutical materials. Using the optimum experimental conditions, quantitative (13)C NMR spectra are then obtained for porous metal-organic frameworks (potential drug carriers) and for components present in drug formulations (active principle ingredient and excipients, amorphous or crystalline). Finally, we show that mixtures of components can also be quantified with this method and, hence, that it represents an ideal tool for quantification of pharmaceutical formulations by (13)C cross-polarization under magic-angle spinning NMR in the industry as it is robust and easy to set up, much faster than direct (13)C polarization and is efficient for samples at natural abundance. PMID:27372550

  1. Basic Wiring. Third Edition. Teacher Edition [and] Student Edition.

    ERIC Educational Resources Information Center

    Kaltwasser, Stan; Flowers, Gary; Blasingame, Don; Batson, Larry; Ipock, Dan; Carroll, Charles; Friesen, Wade; Fleming, Glenn

    This publication contains both a teacher edition and a student edition of materials for a foundation course in an electrical wiring program. The course introduces basic concepts and skills that are prerequisites to residential wiring and commercial and industrial wiring courses. The contents of the materials are tied to measurable and observable…

  2. Major Appliance Repair. Teacher Edition and Student Edition. Second Edition.

    ERIC Educational Resources Information Center

    Smreker, Gene; Calvert, King

    This second edition contains teacher and student guides for 14 units of instruction in major appliance repair. Each unit in the teacher edition includes some or all of the following basic components: objective sheet, suggested activities, answers to assignment sheets, answers to the written test, written test, a unit evaluation form, teacher…

  3. Diesel Technology: Introduction. Teacher Edition [and] Student Edition. Second Edition.

    ERIC Educational Resources Information Center

    Joerschke, John D.; Eichhorn, Lane

    This complete teacher edition of a diesel technology course consists of introductory pages, teacher pages, and the student edition. The introductory pages provide these tools: training and competency profile; National Automotive Technicians Education Foundation Crosswalk; instructional/task analysis; basic skills icons and classifications; basic…

  4. The levels of edit, second edition

    NASA Technical Reports Server (NTRS)

    Vanburen, R.; Buehler, M. F.

    1980-01-01

    The editorial process is analyzed, and five levels of edit are identified. These levels represent cumulative combinations of nine types of edit: Coordination, Policy, Integrity, Screening, Copy Clarification, Format, Mechanical Style, Language, and Substantive. The levels and types of edit, although developed for specific use with external reports at the Jet Propulsion Laboratory, cover the general range of technical editing, especially as it applies to an in-house technical publications organization. Each type of edit is set forth in terms of groups of actions to be performed by editor. The edit-level concept has enhanced understanding and communication among editors, authors, and publications managers concerning the specific editorial work to be done on each manuscript. It has also proved useful as a management tool for estimating and monitoring cost.

  5. Cross-polarized optical absorption of single-walled carbon nanotubes probed by photoluminescence excitation spectroscopy, UV-Vis-IR and polarized Raman Scatterings

    NASA Astrophysics Data System (ADS)

    Maruyama, Shigeo

    2008-03-01

    Because of the depolarization effect, or so-called antenna effect, optical absorption of single-walled carbon nanotubes (SWNTs) is weak when excited by light polarized perpendicular to the nanotube axis. However, in photoluminescence (PL) excitation spectra of isolated SWNTs, PL peaks due to cross-polarized excitation can be clearly identified. By decomposing the cross-polarized component, the optical transition energy of E12 or E21 can be? measured, and the smaller exciton binding energy for perpendicular excitations is concluded [1]. Cross-polarized absorption is dominant in the absorption of a vertically aligned film of SWNTs [2] when excited from the top of the film. In our previous study, a pi-plasmon absorption at 5.25 eV was revealed in contrast to 4.5 eV for parallel excitation [3]. Resonant Raman scattering from such a film is also influenced by the cross-polarized excitation [4]. Even though a Kataura plot for the E33 and E44 range has been proposed by using such a vertically aligned film [5], polarized Raman scattering spectra reveal more complicated features in the system because of the small bundle size, typically 5-8 nanotubes [6]. References: [1] Y. Miyauchi, M. Oba, S. Maruyama, Phys. Rev. B 74 (2006) 205440. [2] Y. Murakami, S. Chiashi, Y. Miyauchi, M. Hu, M. Ogura, T. Okubo, S. Maruyama, Chem. Phys. Lett. 385 (2004) 298. [3] Y. Murakami, E. Einarsson, T. Edamura, S. Maruyama, Phys. Rev. Lett. 94 (2005) 087402. [4] Y. Murakami, S. Chiashi, E. Einarsson, S. Maruyama, Phys. Rev. B 71 (2005) 085403. [5] P. T. Araujo, S. K. Doorn, S. Kilina, S. Tretiak, E. Einarsson, S. Maruyama, H. Chacham, M. A. Pimenta, A. Jorio, Phys. Rev. Lett. 88 (2007) 067401. [6] E. Einarsson, H. Shiozawa, C. Kramberger, M. H. Ruemmeli, A. Gruneis, T. Pichler, S. Maruyama, J. Phys. Chem. C (2007) published on Web.

  6. Statistical study of the effect of ULF fluctuations in the IMF on the cross polar cap potential drop for northward IMF

    NASA Astrophysics Data System (ADS)

    Kim, H.-J.; Lyons, L.; Boudouridis, A.; Pilipenko, V.; Ridley, A. J.; Weygand, J. M.

    2011-10-01

    Recent studies showed that, regardless of the orientation of the Interplanetary Magnetic Field (IMF), ULF wave activity in the solar wind can substantially enhance the convection in the high latitude ionosphere, suggesting that ULF fluctuations may also be an important contributor to the coupling of the solar wind to the magnetosphere-ionosphere system. We conduct a statistical study to understand the effect of ULF power in the IMF on the cross polar cap potential, primarily focusing on northward IMF. We have analyzed the Assimilative Mapping of Ionospheric Electrodynamics (AMIE) calculations of the polar cap potential, a IMF ULF index that is defined as the logarithm of Pc5 ULF power in IMF, and solar wind velocity and dynamic pressure for 249 days in 2003. We find that, separated from the effects of solar wind speed and dynamic pressure, the average cross polar cap potentials show a roughly linear dependence on the ULF index, with a partial correlation coefficient of 0.19. Highly structured convection flow patterns with a number of localized vortices are often observed under fluctuating northward IMF. For such a convection configuration, it is hard to estimate properly the cross polar cap potential drop, as the enhanced flows around the vortices that may be associated with IMF fluctuations do not necessarily yield a large potential drop. Thus, despite the relatively small correlation coefficient, the linear trend we found gives support to the significant role of IMF ULF fluctuations on the coupling of the solar wind to the magnetosphere-ionosphere system.

  7. Wikipedia editing dynamics

    NASA Astrophysics Data System (ADS)

    Gandica, Y.; Carvalho, J.; Sampaio dos Aidos, F.

    2015-01-01

    A model for the probabilistic function followed in editing Wikipedia is presented and compared with simulations and real data. It is argued that the probability of editing is proportional to the editor's number of previous edits (preferential attachment), to the editor's fitness, and to an aging factor. Using these simple ingredients, it is possible to reproduce the results obtained for Wikipedia editing dynamics for a collection of single pages as well as the averaged results. Using a stochastic process framework, a recursive equation was obtained for the average of the number of edits per editor that seems to describe the editing behavior in Wikipedia.

  8. Wikipedia editing dynamics.

    PubMed

    Gandica, Y; Carvalho, J; Sampaio Dos Aidos, F

    2015-01-01

    A model for the probabilistic function followed in editing Wikipedia is presented and compared with simulations and real data. It is argued that the probability of editing is proportional to the editor's number of previous edits (preferential attachment), to the editor's fitness, and to an aging factor. Using these simple ingredients, it is possible to reproduce the results obtained for Wikipedia editing dynamics for a collection of single pages as well as the averaged results. Using a stochastic process framework, a recursive equation was obtained for the average of the number of edits per editor that seems to describe the editing behavior in Wikipedia. PMID:25679673

  9. ST5 Observations of the Imbalance of Region 1 and 2 Field-Aligned Currents and its Implication to the Cross-Polar Cap Pedersen Currents

    NASA Technical Reports Server (NTRS)

    Le, Guan; Slavin, J. A.; Strangeway, Robert

    2010-01-01

    In this study, we use the in-situ magnetic field observations from Space Technology 5 mission to quantify the imbalance of Region 1 (R1) and Region 2 (R2) currents. During the three-month duration of the ST5 mission, geomagnetic conditions range from quiet to moderately active. We find that the R1 current intensity is consistently stronger than the R2 current intensity both for the dawnside and the duskside large-scale field-aligned current system. The net currents flowing into (out of) the ionosphere in the dawnside (duskside) are in the order of 5% of the total R1 currents. We also find that the net currents flowing into or out of the ionosphere are controlled by the solar windmagnetosphere interaction in the same way as the field-aligned currents themselves are. Since the net currents due to the imbalance of the R1 and R2 currents require that their closure currents flow across the polar cap from dawn to dusk as Pedersen currents, our results indicate that the total amount of the cross-polar cap Pedersen currents is in the order of approximately 0.1 MA. This study, although with a very limited dataset, is one of the first attempts to quantify the cross-polar cap Pedersen currents. Given the importance of the Joule heating due to Pedersen currents to the high-latitude ionospheric electrodynamics, quantifying the cross-polar cap Pedersen currents and associated Joule heating is needed for developing models of the magnetosphere-ionosphere coupling.

  10. ST5 Observations of the Imbalance of Region 1 and 2 Field-Aligned Currents and Its Implication to the Cross-Polar Cap Pedersen Currents

    NASA Technical Reports Server (NTRS)

    Le, Guan; Slavin, J. A.; Strangeway, Robert

    2011-01-01

    In this study, we use the in-situ magnetic field observations from Space Technology 5 mission to quantify the imbalance of Region 1 (R1) and Region 2 (R2) currents. During the three-month duration of the ST5 mission, geomagnetic conditions range from quiet to moderately active. We find that the R1 current intensity is consistently stronger than the R2 current intensity both for the dawnside and the duskside large-scale field-aligned current system. The net currents flowing into (out of) the ionosphere in the dawnside (duskside) are in the order of 5% of the total R1 currents. We also find that the net currents flowing into or out of the ionosphere are controlled by the solar wind-magnetosphere interaction in the same way as the field-aligned currents themselves are. Since the net currents due to the imbalance of the R1 and R2 currents require that their closure currents flow across the polar cap from dawn to dusk as Pedersen currents, our results indicate that the total amount of the cross-polar cap Pedersen currents is in the order of 0.1 MA. This study, although with a very limited dataset, is one of the first attempts to quantify the cross-polar cap Pedersen currents. Given the importance of the Joule heating due to Pedersen currents to the high-latitude ionospheric electrodynamics, quantifying the cross-polar cap Pedersen currents and associated Joule heating is needed for developing models of the magnetosphere-ionosphere coupling.

  11. Development of a cross-polarization scattering system for the measurement of internal magnetic fluctuations in the DIII-D tokamak

    SciTech Connect

    Rhodes, T. L. Peebles, W. A.; Crocker, N. A.; Nguyen, X.

    2014-11-15

    The design and performance of a new cross-polarization scattering (CPS) system for the localized measurement of internal magnetic fluctuations is presented. CPS is a process whereby magnetic fluctuations scatter incident electromagnetic radiation into a perpendicular polarization which is subsequently detected. A new CPS design that incorporates a unique scattering geometry was laboratory tested, optimized, and installed on the DIII-D tokamak. Plasma tests of signal-to-noise, polarization purity, and frequency response indicate proper functioning of the system. CPS data show interesting features related to internal MHD perturbations known as sawteeth that are not observed on density fluctuations.

  12. Protonation of carbon single-walled nanotubes studied using 13C and 1H-13C cross polarization nuclear magnetic resonance and Raman spectroscopies.

    PubMed

    Engtrakul, Chaiwat; Davis, Mark F; Gennett, Thomas; Dillon, Anne C; Jones, Kim M; Heben, Michael J

    2005-12-14

    The reversible protonation of carbon single-walled nanotubes (SWNTs) in sulfuric acid and Nafion was investigated using solid-state nuclear magnetic resonance (NMR) and Raman spectroscopies. Magic-angle spinning (MAS) was used to obtain high-resolution 13C and 1H-13C cross polarization (CP) NMR spectra. The 13C NMR chemical shifts are reported for bulk SWNTs, H2SO4-treated SWNTs, SWNT-Nafion polymer composites, SWNT-AQ55 polymer composites, and SWNTs in contact with water. Protonation occurs without irreversible oxidation of the nanotube substrate via a charge-transfer process. This is the first report of a chemically induced change in a SWNT 13C resonance brought about by a reversible interaction with an acidic proton, providing additional evidence that carbon nanotubes behave as weak bases. Cross polarization was found to be a powerful technique for providing an additional contrast mechanism for studying nanotubes in contact with other chemical species. The CP studies confirmed polarization transfer from nearby protons to nanotube carbon atoms. The CP technique was also applied to investigate water adsorbed on carbon nanotube surfaces. Finally, the degree of bundling of the SWNTs in Nafion films was probed with the 1H-13C CP-MAS technique. PMID:16332107

  13. Rapid solid-state NMR of deuterated proteins by interleaved cross-polarization from 1H and 2H nuclei

    NASA Astrophysics Data System (ADS)

    Bjerring, Morten; Paaske, Berit; Oschkinat, Hartmut; Akbey, Ümit; Nielsen, Niels Chr.

    2012-01-01

    We present a novel sampling strategy, interleaving acquisition of multiple NMR spectra by exploiting initial polarization subsequently from 1H and 2H spins, taking advantage of their different T1 relaxation times. Different 1H- and 2H-polarization based spectra are in this way simultaneously recorded improving either information content or sensitivity by adding spectra. The so-called Relaxation-optimized Acquisition of Proton Interleaved with Deuterium (RAPID) 1H → 13C/ 2H → 13C CP/MAS multiple-acquisition method is demonstrated by 1D and 2D experiments using a uniformly 2H, 15N, 13C-labeled α-spectrin SH3 domain sample with all or 30% back-exchanged labile 2H to 1H. It is demonstrated how 1D 13C CP/MAS or 2D 13C- 13C correlation spectra initialized with polarization from either 1H or 2H may be recorded simultaneously with flexibility to be added or used individually for spectral editing. It is also shown how 2D 13C- 13C correlation spectra may be recorded interleaved with 2H- 13C correlation spectra to obtain 13C- 13C correlations along with information about dynamics from 2H sideband patterns.

  14. Developing new levels of edit

    SciTech Connect

    Prono, J.; DeLanoy, M.; Deupree, R.; Skiby, J.; Thompson, B.

    1998-07-01

    In 1985, the writing and editing group at Los Alamos National Laboratory established four levels of edit for technical reports. When a survey in 1994 showed that both authors and editors felt the levels were not meeting author needs, the authors set about revising them. Their goals were to simplify the editing process, focus editing on improving technical clarity, and ensure that value was added in editing. This paper describes the revision process and product -- three author-based levels of edit.

  15. Digital Video Editing

    ERIC Educational Resources Information Center

    McConnell, Terry

    2004-01-01

    Monica Adams, head librarian at Robinson Secondary in Fairfax country, Virginia, states that librarians should have the technical knowledge to support projects related to digital video editing. The process of digital video editing and the cables, storage issues and the computer system with software is described.

  16. Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles.

    PubMed

    Koroloff, Sophie N; Nevzorov, Alexander A

    2015-07-01

    Solid-state NMR (ssNMR) of oriented membrane proteins (MPs) is capable of providing structural and dynamic information at nearly physiological conditions. However, NMR experiments performed on oriented membrane proteins generally suffer from low sensitivity. Moreover, utilization of high-power radiofrequency (RF) irradiations for magnetization transfer may give rise to sample heating, thereby decreasing the efficiency of conventional cross-polarization schemes. Here we have optimized the recently developed repetitive cross-polarization (REP-CP) sequence (Tang et al., 2011) to further increase the magnetization transfer efficiency for membrane proteins reconstituted in magnetically aligned bicelles and compared its performance to single-contact Hartmann-Hahn cross-polarization (CP), CP-MOIST and the adiabatic transfer. It has been found that employing the REP-CP sequence at RF amplitudes of 19kHz instead of the commonly used higher RF fields (>45kHz) enhances the efficiency of REP-CP. An additional 30% signal can be obtained as compared to the previously published REP-CP, and 20% when compared to the re-optimized REP-CP at 50kHz RF fields. Moreover, the (15)N signal gain of low-power REP-CP was found to be 40% over the adiabatic CP and up to 80% over CP-MOIST. Thus, the low-power REP-CP sequence surpasses all of the previous CP schemes in addition of having the tremendous advantage of reducing the RF powers by a factor of seven, thereby preserving the liquid-like bicelle sample. By contrast, in purely static (NAL crystal) and semi-rigid systems (Pf1 phage), the adiabatic CP was found to be more effective. Periodic oscillations of the intensity profile (distinct from the transient oscillations) as a function of the CP contact time and B1 RF field strengths were observed during the REP-CP optimization with the oscillations becoming more pronounced with lower RF fields. Many-spin simulations were performed to explain the oscillations and their periodicity. PMID

  17. Wavelength reuse in a bidirectional radio-over-fiber link based on cross-gain and cross-polarization modulation in a semiconductor optical amplifier.

    PubMed

    Zhu, Beibei; Pan, Shilong; Zhu, Dan; Yao, Jianping

    2013-09-15

    Wavelength reuse in a bidirectional radio-over-fiber link is proposed and demonstrated based on the cross-gain modulation (XGM) and the cross-polarization modulation in a semiconductor optical amplifier (SOA). With a polarization beam splitter placed at the remote antenna unit, the polarization-modulated signal generated at the SOA is converted into noninverted and inverted intensity-modulated signals. The noninverted signal is used to cancel the inverted XGM-induced intensity-modulated signal to form a clean optical carrier for wavelength reuse in upstream signal transmission, while the inverted intensity-modulated signal is combined constructively to enhance the XGM-induced signal, providing robust downlink service. A bidirectional transmission of 5 GHz RF signal carrying a 50 MBaud 16 quadrature amplitude modulation baseband signal is experimentally implemented. The error vector magnitude degradation due to the fiber transmission for both the downlink and uplink signal is about 0.2%. PMID:24104797

  18. Marine target detection in quad-pol synthetic aperture radar imagery based on the relative phase of cross-polarized channels

    NASA Astrophysics Data System (ADS)

    Wang, Yunhua; Li, Huimin; Zhang, Yanmin; Guo, Lixin

    2015-01-01

    A focus on marine target detection in noise corrupted fully polarimetric synthetic aperture radar (SAR) is presented. The property of the relative phase between two cross-polarized channels reveals that the relative phases evaluated within sea surface area or noise corrupted area are widely spread phase angle region [-π,π] due to decorrelation effect; however, the relative phases are concentrated to zero and ±π for real target and its first-order azimuth ambiguities (FOAAs), respectively. Exploiting this physical behavior, the reciprocal of the mean square value of the relative phase (RMSRP) is defined as a new parameter for target detection, and the experiments based on fully polarimetric Radarsat-2 SAR images show that the strong noise and the FOAAs can be effectively suppressed in RMSRP image. Meanwhile, validity of the new parameter for target detection is also verified by two typical Radarsat-2 SAR images, in which targets' ambiguities and strong noise are present.

  19. Use of 2D images of depth and integrated reflectivity to represent the severity of demineralization in cross-polarization optical coherence tomography.

    PubMed

    Chan, Kenneth H; Chan, Andrew C; Fried, William A; Simon, Jacob C; Darling, Cynthia L; Fried, Daniel

    2015-01-01

    Several studies have demonstrated the potential of cross-polarization optical coherence tomography (CP-OCT) to quantify the severity of early caries lesions (tooth decay) on tooth surfaces. The purpose of this study is to show that 2D images of the lesion depth and the integrated reflectivity can be used to accurately represent the severity of early lesions. Simulated early lesions of varying severity were produced on tooth samples using simulated lesion models. Methods were developed to convert the 3D CP-OCT images of the samples to 2D images of the lesion depth and lesion integrated reflectivity. Calculated lesion depths from OCT were compared with lesion depths measured from histological sections examined using polarized light microscopy. The 2D images of the lesion depth and integrated reflectivity are well suited for visualization of early demineralization. PMID:24307350

  20. Understanding and optimizing microstrip patch antenna cross polarization radiation on element level for demanding phased array antennas in weather radar applications

    NASA Astrophysics Data System (ADS)

    Vollbracht, D.

    2015-11-01

    The antenna cross polarization suppression (CPS) is of significant importance for the accurate calculation of polarimetric weather radar moments. State-of-the-art reflector antennas fulfill these requirements, but phased array antennas are changing their CPS during the main beam shift, off-broadside direction. Since the cross polarization (x-pol) of the array pattern is affected by the x-pol element factor, the single antenna element should be designed for maximum CPS, not only at broadside, but also for the complete angular electronic scan (e-scan) range of the phased array antenna main beam positions. Different methods for reducing the x-pol radiation from microstrip patch antenna elements, available from literature sources, are discussed and summarized. The potential x-pol sources from probe fed microstrip patch antennas are investigated. Due to the lack of literature references, circular and square shaped X-Band radiators are compared in their x-pol performance and the microstrip patch antenna size variation was analyzed for improved x-pol pattern. Furthermore, the most promising technique for the reduction of x-pol radiation, namely "differential feeding with two RF signals 180° out of phase", is compared to single fed patch antennas and thoroughly investigated for phased array applications with simulation results from CST MICROWAVE STUDIO (CST MWS). A new explanation for the excellent port isolation of dual linear polarized and differential fed patch antennas is given graphically. The antenna radiation pattern from single fed and differential fed microstrip patch antennas are analyzed and the shapes of the x-pol patterns are discussed with the well-known cavity model. Moreover, two new visual based electromagnetic approaches for the explanation of the x-pol generation will be given: the field line approach and the surface current distribution approach provide new insight in understanding the generation of x-pol component in microstrip patch antenna radiation

  1. CRISPR Genome Editing

    Cancer.gov

    A research article about a technique for gene editing known as CRISPR-Cas9. The technique has made it much easier and faster for cancer researchers to study mutations and test new therapeutic targets.

  2. Developing new levels of edit

    SciTech Connect

    Prono, J.K.

    1997-06-01

    Since 1985, Los Alamos National Laboratory (LANL) staff have had four levels of edit to choose from for technical reports. When a CQI survey showed that both authors and editors felt the levels were not meeting author needs, LANL set about revising them. The goals were to simplify the editing process, focus editing on improving technical clarity, and ensure value added in editing. This paper describes the revision process and product--three author-based levels of edit.

  3. Modular video endoscopy for in vivo cross-polarized and vital-dye fluorescence imaging of Barrett’s-associated neoplasia

    PubMed Central

    Pierce, Mark C.; Lee, Michelle H.; Polydorides, Alexandros D.; Flores, Raja M.; Anandasabapathy, Sharmila; Richards-Kortum, Rebecca R.

    2013-01-01

    Abstract. A modular video endoscope is developed and tested to allow imaging in different modalities. This system incorporates white light imaging (WLI), cross-polarized imaging (CPI), and vital-dye fluorescence imaging (VFI), using interchangeable filter modules. CPI and VFI are novel endoscopic modalities that probe mucosal features associated with Barrett’s neoplasia. CPI enhances vasculature, while VFI enhances glandular architecture. In this pilot study, we demonstrate the integration of these modalities by imaging areas of Barrett’s metaplasia and neoplasia in an esophagectomy specimen. We verify that those key image features are also observed during an in vivo surveillance procedure. CPI images demonstrate improved visualization of branching blood vessels associated with neoplasia. VFI images show glandular architecture with increased glandular effacement associated with neoplasia. Results suggests that important pathologic features seen in CPI and VFI are not visible during standard endoscopic white light imaging, and thus the modalities may be useful in future in vivo studies for discriminating neoplasia from Barrett’s metaplasia. We further demonstrate that the integrated WLI/CPI/VFI endoscope is compatible with complementary high-resolution endomicroscopy techniques such as the high-resolution microendoscope, potentially enabling two-step (“red-flag” widefield plus confirmatory high-resolution imaging) protocols to be enhanced. PMID:23370452

  4. Cross-polarization magic-angle spinning nuclear magnetic resonance study of platinum complexes containing the cis-P2PtC2 fragment.

    PubMed

    Challoner, R; Sebald, A

    1995-01-01

    31P and 195Pt cross-polarization magic-angle spinning nuclear magnetic resonance (CP-MAS NMR) spectra of three platinum complexes of formal oxidation state Pt(0) and Pt(II), respectively, are reported. All three complexes, (Ph3P)2Pt(C2H4) (1), (Et2P-CH2-CH2-PEt2)Pt(C identical to C-H)2 (2) and (Ph2P-CH2-CH2-PPh2)Pt(C identical to C-C(CH3)=CH2)2 (3) contain the square-planar cis-P2PtC2 fragment and show unusual NMR spectroscopic properties insofar that the 195Pt shielding patterns are fairly narrow in relation to what one would generally have to expect for 195Pt in square-planar coordination. Another unexpected NMR property of the cis-P2PtC2 fragment in 1-3 is the absence of spinning frequency-dependent second-order effects in this solid-state ABX spin system. PMID:7894980

  5. Broadband photonic single sideband frequency up-converter based on the cross polarization modulation effect in a semiconductor optical amplifier for radio-over-fiber systems.

    PubMed

    Lee, Seung-Hun; Kim, Hyoung-Jun; Song, Jong-In

    2014-01-13

    A broadband photonic single sideband (SSB) frequency up-converter based on the cross polarization modulation (XPolM) effect in a semiconductor optical amplifier (SOA) is proposed and experimentally demonstrated. An optical radio frequency (RF) signal in the form of an optical single sideband (OSSB) is generated by the photonic SSB frequency up-converter to solve the power fading problem caused by fiber chromatic dispersion. The generated OSSB RF signal has almost identical optical carrier power and optical sideband power. This SSB frequency up-conversion scheme shows an almost flat electrical RF power response as a function of the RF frequency in a range from 31 GHz to 75 GHz after 40 km single mode fiber (SMF) transmission. The photonic SSB frequency up-conversion technique shows negligible phase noise degradation. The phase noise of the up-converted RF signal at 49 GHz for an offset of 10 kHz is -93.17 dBc/Hz. Linearity analysis shows that the photonic SSB frequency up-converter has a spurious free dynamic range (SFDR) value of 79.51 dB · Hz(2/3). PMID:24514980

  6. Editing graphs for maximum effect

    SciTech Connect

    Murphy, P.W.; Rhiner, R.W.

    1991-01-08

    The paper contains over eighty rules for editing graphs, arranged under nine major headings in a logical sequence for editing all the graphs in a manuscript. It is excerpted from a monograph used at the Lawrence Livermore National Laboratory to train beginning technical editors in editing graphs; a corresponding Hypercard stack is also used in this training. 6 refs., 4 figs.

  7. MENTAL DEFICIENCY. SECOND EDITION.

    ERIC Educational Resources Information Center

    HILLIARD, L.T.; KIRMAN, BRIAN H.

    REVISED TO INCLUDE LEGISLATIVE AND ADMINISTRATIVE PROCEDURES NEW IN BRITAIN SINCE THE 1957 EDITION, THE TEXT INCLUDES RECENT ADVANCES IN ETIOLOGY, PATHOLOGY, AND TREATMENT OF MENTAL DEFICIENCY. CONSIDERATION OF THE BACKGROUND OF MENTAL DEFICIENCY INCLUDES HISTORICAL AND LEGAL ASPECTS, THE SOCIAL BACKGROUND OF MENTAL DEFECT, PRENATAL CAUSES OF…

  8. Behaviour Recovery. Second Edition

    ERIC Educational Resources Information Center

    Rogers, Bill

    2004-01-01

    This second edition of Behaviour Recovery puts emphasis on teaching behaviour concerning children with emotional and behavioural disorders (EBD). These children have many factors in their lives that affect their behaviour over which schools have limited control. This book acknowledges the challenge and explores the practical realities, options and…

  9. Beginning to edit physics

    SciTech Connect

    Murphy, P.W.

    1995-02-01

    A physicist-turned-editor shows you the basics required for copyediting physics papers (physical quantities, symbols, units, scientific notation, the structure of mathematical expressions, the nature of graphs), and points the way to learning enough ``editorial physics`` to begin substantive editing.

  10. Contaminant hydrogeology. 2. edition

    SciTech Connect

    Fetter, C.W.

    1999-08-01

    This book is a major revision of the 1993 edition and includes the important practical concepts that have since emerged. Contents include: mass transport in saturated media; transformation, retardation, and attenuation of solutes; flow and mass transport in the vadose zone; multiphase flow; inorganic chemicals in groundwater; organic compounds in groundwater; groundwater and soil monitoring; and site remediation.

  11. Editing Technical Writing.

    ERIC Educational Resources Information Center

    Samson, Donald C., Jr.

    Intended for students in upper-division technical communication courses and professionals in business and government who want to learn how to edit technical writing, this book describes what technical editors do and how they do it. Throughout the book are exercises that students can use as self-tests; answer keys are provided for checking work.…

  12. Characterization of high-tannin fractions from humus by carbon-13 cross-polarization and magic-angle spinning nuclear magnetic resonance.

    PubMed

    Lorenz, Klaus; Preston, Caroline M

    2002-01-01

    Condensed tannins can be found in various parts of many plants. Unlike lignin there has been little study of their fate as they enter the soil organic matter pool and their influence on nutrient cycling, especially through their protein-binding properties. We extracted and characterized tannin-rich fractions from humus collected in 1998 from a black spruce [Picea mariana (Mill.) Britton et al.] forest in Canada where a previous study (1995) showed high levels (3.8% by weight) of condensed tannins. A reference tannin purified from black spruce needles was characterized by solution 13C nuclear magnetic resonance (NMR) as a pure procyanidin with mainly cis stereochemistry and an average chain length of four to five units. The colorimetric proanthocyanidin (PA) assay, standardized against the black spruce tannin, showed that both extracted humus fractions had higher tannin contents than the original humus (2.84% and 11.17% vs. 0.08%), and accounted for 32% of humus tannin content. Consistent with the results from the chemical assay, the aqueous fraction showed higher tannin signals in the 13C cross-polarization and magic-angle spinning (CPMAS) NMR spectrum than the emulsified one. As both tannin-rich humus fractions were depleted in N and high in structures derived from lignin and cutin, they did not have properties consistent with recaldtrant tannin-protein complexes proposed as a mechanism for N sequestration in humus. Further studies are needed to establish if tannin-protein structures in humus can be detected or isolated, or if tannins contribute to forest management problems observed in these ecosystems by binding to and slowing down the activity of soil enzymes. PMID:11931430

  13. Carbon-13 cross-polarization magic-angle-spinning nuclear magnetic resonance investigation of the interactions between maleic anhydride grafted polypropylene and wood polymers.

    PubMed

    Rude, Erica; Laborie, Marie-Pierre G

    2008-05-01

    The chemical interactions between maleic anhydride grafted polypropylene (MAPP) and wood were studied with solid-state carbon-13 cross-polarization magic-angle-spinning nuclear magnetic resonance ((13)C CPMAS NMR) spectroscopy. MAPP was synthesized with 100% (13)C enrichment at the C(1) and C(4) carbons to allow detection of the [1,4-(13)C(2)]MAPP functional groups and was melt blended with cellulose, lignin, and maple wood. In the cellulose/MAPP blend, changes in (13)C CPMAS NMR corrected signal intensities for the anhydride and dicarboxylic maleic acid functionalities suggested that esterification may have occurred predominantly from the more numerous diacid carbons. A single proton longitudinal relaxation in the rotating frame, (H)T(1rho), for the MAPP and the cellulose carbons in the blend suggested that they were spin coupled, i.e., homogeneous on a 10-200 Angstrom scale. Esterification was also suggested in the lignin/MAPP blend. Furthermore, the more significant changes in the intensities of the carbonyl signals and (H)T(1rho) values suggested that lignin may be more reactive to MAPP than cellulose. Finally, when maple was melt blended with MAPP, the same trends in the (13)C CP-MAS NMR spectra and (H)T(1rho) behavior were observed as when MAPP was blended with cellulose or lignin. This study therefore clarifies that during melt compounding of wood with MAPP, esterification occurs with wood polymers, preferentially with lignin. Understanding the interactions of MAPP with wood is of significance for the development of natural-fiber-reinforced thermoplastic composites. PMID:18498698

  14. Video Editing System

    NASA Technical Reports Server (NTRS)

    Schlecht, Leslie E.; Kutler, Paul (Technical Monitor)

    1998-01-01

    This is a proposal for a general use system based, on the SGI IRIS workstation platform, for recording computer animation to videotape. In addition, this system would provide features for simple editing and enhancement. Described here are a list of requirements for the system, and a proposed configuration including the SGI VideoLab Integrator, VideoMedia VLAN animation controller and the Pioneer rewritable laserdisc recorder.

  15. Comparisons of lipid dynamics and packing in fully interdigitated monoarachidoylphosphatidylcholine and non-interdigitated dipalmitoylphosphatidylcholine bilayers: cross polarization/magic angle spinning 13C-NMR studies.

    PubMed

    Wu, W G; Chi, L M

    1990-07-24

    13C-NMR spectra have been obtained at 50.3 MHz for monoarachidoylphosphatidylcholine (MAPC) and dipalmitoylphosphatidylcholine (DPPC) dispersions from 25 degrees C to 55 degrees C and for DPPC polycrystals at 25 degrees C using the cross polarization/magic angle spinning technique. Differential scanning calorimetric studies on DPPC and MAPC dispersions show comparable lipid phase transitions with transition temperatures at 41 degrees C and 45 degrees C, respectively, and thus enable the comparison of thermal, structural and dynamic differences between these two systems at corresponding temperatures. Conformational-dependent 13C chemical shift studies on DPPC dispersions demonstrate not only the coexistence of the tilted gel (L beta') and liquid-crystalline (L alpha) phases in the rippled gel (P beta') phase, but also the presence of an intermediate third microscopic phase as evidenced by three resolvable peaks for omega - 1 methylene carbon signals at the temperature interval between Tp and Tm. By comparing chemical shifts of MAPC in the hydrocarbon chain region with those of DPPC at similar reduced temperatures, it can be concluded that the packings are perturbed markedly in the middle segment of the fatty acyl chain during the lamellar to micellar transition. However, terminal methylene and methyl groups of interdigitated MAPC lamellae were found to be more ordered than those of non-interdigitated DPPC bilayers in the gel state. Interestingly, the terminal methyl groups of MAPC in the micelles remain to be relatively ordered; in fact, they are more ordered than the corresponding acyl chain end of DPPC in the liquid-crystalline state. Analysis of data obtained from rotating frame proton spin-lattice relaxation measurements shows a highly mobile phosphocholine headgroup, a rigid carbonyl group and an ordered hydrocarbon chain for lamellar MAPC in the interdigitated state. Furthermore, results suggest that free rotations of the glycerol C2-C3 bond within MAPC

  16. REDIdb: the RNA editing database

    PubMed Central

    Picardi, Ernesto; Regina, Teresa Maria Rosaria; Brennicke, Axel; Quagliariello, Carla

    2007-01-01

    The RNA Editing Database (REDIdb) is an interactive, web-based database created and designed with the aim to allocate RNA editing events such as substitutions, insertions and deletions occurring in a wide range of organisms. The database contains both fully and partially sequenced DNA molecules for which editing information is available either by experimental inspection (in vitro) or by computational detection (in silico). Each record of REDIdb is organized in a specific flat-file containing a description of the main characteristics of the entry, a feature table with the editing events and related details and a sequence zone with both the genomic sequence and the corresponding edited transcript. REDIdb is a relational database in which the browsing and identification of editing sites has been simplified by means of two facilities to either graphically display genomic or cDNA sequences or to show the corresponding alignment. In both cases, all editing sites are highlighted in colour and their relative positions are detailed by mousing over. New editing positions can be directly submitted to REDIdb after a user-specific registration to obtain authorized secure access. This first version of REDIdb database stores 9964 editing events and can be freely queried at . PMID:17175530

  17. Editing the Small Magazine. 2nd Edition Revised.

    ERIC Educational Resources Information Center

    Ferguson, Rowena

    Designed for the aspiring journalist and the professional editor, this revised second edition serves as a standard reference work as well as an ideal means of keeping abreast of new trends and developments in the editing of small magazines. It describes the latest technological developments in type-setting and production; discusses and evaluates…

  18. Direct volume editing.

    PubMed

    Bürger, Kai; Krüger, Jens; Westermann, Rüdiger

    2008-01-01

    In this work we present basic methodology for interactive volume editing on GPUs, and we demonstrate the use of these methods to achieve a number of different effects. We present fast techniques to modify the appearance and structure of volumetric scalar fields given on Cartesian grids. Similar to 2D circular brushes as used in surface painting we present 3D spherical brushes for intuitive coloring of particular structures in such fields. This paint metaphor is extended to allow the user to change the data itself, and the use of this functionality for interactive structure isolation, hole filling, and artefact removal is demonstrated. Building on previous work in the field we introduce high-resolution selection volumes, which can be seen as a resolution-based focus+context metaphor. By utilizing such volumes we present a novel approach to interactive volume editing at sub-voxel accuracy. Finally, we introduce a fast technique to paste textures onto iso-surfaces in a 3D scalar field. Since the texture resolution is independent of the volume resolution, this technique allows structure-aligned textures containing appearance properties or textual information to be used for volume augmentation and annotation. PMID:18988988

  19. Natural Hazards, Second Edition

    NASA Astrophysics Data System (ADS)

    Rouhban, Badaoui

    Natural disaster loss is on the rise, and the vulnerability of the human and physical environment to the violent forces of nature is increasing. In many parts of the world, disasters caused by natural hazards such as earthquakes, floods, landslides, drought, wildfires, intense windstorms, tsunami, and volcanic eruptions have caused the loss of human lives, injury, homelessness, and the destruction of economic and social infrastructure. Over the last few years, there has been an increase in the occurrence, severity, and intensity of disasters, culminating with the devastating tsunami of 26 December 2004 in South East Asia.Natural hazards are often unexpected or uncontrollable natural events of varying magnitude. Understanding their mechanisms and assessing their distribution in time and space are necessary for refining risk mitigation measures. This second edition of Natural Hazards, (following a first edition published in 1991 by Cambridge University Press), written by Edward Bryant, associate dean of science at Wollongong University, Australia, grapples with this crucial issue, aspects of hazard prediction, and other issues. The book presents a comprehensive analysis of different categories of hazards of climatic and geological origin.

  20. Children in Sport. Second Edition.

    ERIC Educational Resources Information Center

    Magill, Richard A., Ed.; And Others

    This anthology of papers is designed to serve both as a textbook and as a synthesis of research efforts in youth sport. Half of the 20 papers are reprinted from the first edition of this volume; of the remainder, some were written especially for this edition, while others are culled from journals and conference proceedings. Five subject sections…

  1. Drug Abuse Films, Second Edition.

    ERIC Educational Resources Information Center

    National Coordinating Council on Drug Education, Washington, DC.

    This second edition updates and expands a 1971 evaluation of films and audiovisuals related to drug education performed by the National Coordinating Council on Drug Education. Materials in this edition are evaluated both for accuracy and effectiveness as a communications tool. They are separated into two sections--films and other audiovisuals…

  2. Exploring the conformational energy landscape of glassy disaccharides by cross polarization magic angle spinning 13C nuclear magnetic resonance and numerical simulations. II. Enhanced molecular flexibility in amorphous trehalose

    NASA Astrophysics Data System (ADS)

    Lefort, Ronan; Bordat, Patrice; Cesaro, Attilio; Descamps, Marc

    2007-01-01

    This paper uses chemical shift surfaces to simulate experimental C13 cross polarization magic angle spinning spectra for amorphous solid state disaccharides, paying particular attention to the glycosidic linkage atoms in trehalose, sucrose, and lactose. The combination of molecular mechanics with density functional theory/gauge invariant atomic orbital ab initio methods provides reliable structural information on the conformational distribution in the glass. The results are interpreted in terms of an enhanced flexibility that trehalose possesses in the amorphous solid state, at least on the time scale of C13 nuclear magnetic resonance measurements. Implications of these findings for the fragility of trehalose glass and bioprotectant action are discussed.

  3. Residential and Light Commercial HVAC. Teacher Edition and Student Edition. Second Edition.

    ERIC Educational Resources Information Center

    Stephenson, David

    This package contains teacher and student editions of a residential and light commercial heating, ventilation, and air conditioning (HVAC) course of study. The teacher edition contains information on the following: using the publication; national competencies; competency profile; related academic and workplace skills list; tools, equipment, and…

  4. Graphic Arts: Process Camera, Stripping, and Platemaking. Fourth Edition. Teacher Edition [and] Student Edition.

    ERIC Educational Resources Information Center

    Multistate Academic and Vocational Curriculum Consortium, Stillwater, OK.

    This publication contains both a teacher edition and a student edition of materials for a course in graphic arts that covers the process camera, stripping, and platemaking. The course introduces basic concepts and skills necessary for entry-level employment in a graphic communication occupation. The contents of the materials are tied to measurable…

  5. Oxyacetylene Welding and Oxyfuel Cutting. Third Edition. Teacher Edition [and] Student Edition [and] Student Workbook.

    ERIC Educational Resources Information Center

    Knapp, John; Harper, Eddie

    This Oklahoma curriculum guide, which includes a teacher edition, a student edition, and a student workbook, provides three units for a course on oxyacetylene welding, oxyfuel cutting, and cutting done with alternative fuels such as MAPP, propane, and natural gas. The three units are: "Oxyacetylene Welding"; "Oxyfuel Cutting"; and "Oxyacetylene…

  6. Fundamentals of Welding. Teacher Edition [and] Student Edition [and] Student Workbook. Second Edition.

    ERIC Educational Resources Information Center

    Fortney, Clarence; Gregory, Mike; New, Larry

    Teacher and student editions and a student workbook for fundamentals of welding comprise the first of six in a series of competency-based instructional materials for welding programs. Introductory pages in the teacher edition are training and competency profile, instructional/task analysis, basic skills icons and classifications, basic skills…

  7. Introduction to Surgical Technology. Third Edition. Teacher Edition [and] Student Edition.

    ERIC Educational Resources Information Center

    Bushey, Vicki; Hildebrand, Bob; Hildebrand, Dinah; Johnson, Dave; Sikes, John; Tahah, Ann; Walker, Susan; Zielsdorf, Lani

    These teacher and student editions provide instructional materials for an introduction to surgical technology course. Introductory materials in the teacher edition include information on use, instructional/task analysis, academic and workplace skill classifications and definitions, related academic and workplace skill list, and crosswalk to…

  8. Ethics manual: fifth edition.

    PubMed

    Snyder, Lois; Leffler, Cathy

    2005-04-01

    Medicine, law, and social values are not static. Reexamining the ethical tenets of medical practice and their application in new circumstances is a necessary exercise. The fifth edition of the College's Ethics Manual covers emerging issues in medical ethics and revisits old ones. It reflects on many of the ethical tensions faced by internists and their patients and attempts to shed light on how existing principles extend to emerging concerns. In addition, by reiterating ethical principles that have provided guidance in resolving past ethical problems, the Manual may help physicians avert future problems. The Manual is not a substitute for the experience and integrity of individual physicians, but it may serve as a reminder of the shared obligations and duties of the medical profession. PMID:15809467

  9. Tool steels. 5. edition

    SciTech Connect

    Roberts, G.; Krauss, G.; Kennedy, R.

    1998-12-31

    The revision of this authoritative work contains a significant amount of new information from the past nearly two decades presented in an entirely new outline, making this a must have reference for engineers involved in tool-steel production, as well as in the selection and use of tool steels in metalworking and other materials manufacturing industries. The chapter on tool-steel manufacturing includes new production processes, such as electroslag refining, vacuum arc remelting, spray deposition processes (Osprey and centrifugal spray), and powder metal processing. The seven chapters covering tool-steel types in the 4th Edition have been expanded to 11 chapters covering nine main groups of tool steels as well as other types of ultrahigh strength steels sometimes used for tooling. Each chapter discusses in detail processing, composition, and applications specific to the particular group. In addition, two chapters have been added covering surface modification and trouble shooting production and performance problems.

  10. Volcanoes, Third Edition

    NASA Astrophysics Data System (ADS)

    Nye, Christopher J.

    It takes confidence to title a smallish book merely “Volcanoes” because of the impliction that the myriad facets of volcanism—chemistry, physics, geology, meteorology, hazard mitigation, and more—have been identified and addressed to some nontrivial level of detail. Robert and Barbara Decker have visited these different facets seamlessly in Volcanoes, Third Edition. The seamlessness comes from a broad overarching, interdisciplinary, professional understanding of volcanism combined with an exceptionally smooth translation of scientific jargon into plain language.The result is a book which will be informative to a very broad audience, from reasonably educated nongeologists (my mother loves it) to geology undergraduates through professional volcanologists. I bet that even the most senior professional volcanologists will learn at least a few things from this book and will find at least a few provocative discussions of subjects they know.

  11. Practical writing and editing techniques

    SciTech Connect

    Hallinan, E.J.

    1984-01-01

    Technical writing and editing involve more than a knowledge of style and format. Often a draft must be prepared from existing documents and edited for content as well as style. A practical approach to writing and editing tasks includes increasing writer/editor exposure with cognizant personnel, researching source and legal documents, participating in project meetings, and reviewing the document by checking for inconsistencies and selectively questioning the contents. This approach will help the writer/editor prepare a reasonably accurate, politically acceptable document with a minimum of supervision.

  12. Edit while watching: home video editing made easy

    NASA Astrophysics Data System (ADS)

    Campanella, Marco; Weda, Hans; Barbieri, Mauro

    2007-01-01

    In recent years, more and more people capture their experiences in home videos. However, home video editing still is a difficult and time-consuming task. We present the Edit While Watching system that allows users to automatically create and change a summary of a home video in an easy, intuitive and lean-back way. Based on content analysis, video is indexed, segmented, and combined with proper music and editing effects. The result is an automatically generated home video summary that is shown to the user. While watching it, users can indicate whether they like certain content, so that the system will adapt the summary to contain more content that is similar or related to the displayed content. During the video playback users can also modify and enrich the content, seeing immediately the effects of their changes. Edit While Watching does not require a complex user interface: a TV and a few keys of a remote control are sufficient. A user study has shown that it is easy to learn and to use, even if users expressed the need for more control in the editing operations and in the editing process.

  13. Water quality management library. 2. edition

    SciTech Connect

    Eckenfelder, W.W.; Malina, J.F.; Patterson, J.W.

    1998-12-31

    A series of ten books offered in conjunction with Water Quality International, the Biennial Conference and Exposition of the International Association on Water Pollution Research and Control (IAWPRC). Volume 1, Activated Sludge Process, Design and Control, 2nd edition, 1998: Volume 2, Upgrading Wastewater Treatment Plants, 2nd edition, 1998: Volume 3, Toxicity Reduction, 2nd edition, 1998: Volume 4, Municipal Sewage Sludge Management, 2nd edition, 1998: Volume 5, Design and Retrofit of Wastewater Treatment Plants for Biological Nutrient Removal, 1st edition, 1992: Volume 6, Dynamics and Control of the Activated Sludge Process, 2nd edition, 1998: Volume 7: Design of Anaerobic Processes for the Treatment of Industrial and Municipal Wastes, 1st edition, 1992: Volume 8, Groundwater Remediation, 1st edition, 1992: Volume 9, Nonpoint Pollution and Urban Stormwater Management, 1st edition, 1995: Volume 10, Wastewater Reclamation and Reuse, 1st edition, 1998.

  14. Marine botany. Second edition

    SciTech Connect

    Dawes, C.J.

    1998-12-01

    Marine plants are a diverse group that include unicellular algae, seaweeds, seagrasses, salt marshes, and mangrove forests. They carry out a variety of ecological functions and serve as the primary producers in coastal wetlands and oceanic waters. The theme that connects such a wide variety of plants is their ecology, which was also emphasized in the 1981 edition. The goal of this revision is to present taxonomic, physiological, chemical, and ecological aspects of marine plants, their adaptations, and how abiotic and biotic factors interact in their communities. The data are presented in a concise, comparative manner in order to identify similarities and differences between communities such as salt marsh and mangroves or subtidal seaweeds and seagrasses. To accomplish this, the text is organized into five chapters that introduce the marine habitats, consider abiotic and biotic factors, and anthropogenic influences on the communities followed by seven chapters that deal with microalgae, seaweeds, salt marshes, mangroves, seagrasses, and coral reefs. Two appendixes are included; one presents simple field techniques and the other is a summary of seaweed uses.

  15. To edit or not to edit: regulation of ADAR editing specificity and efficiency.

    PubMed

    Deffit, Sarah N; Hundley, Heather A

    2016-01-01

    Hundreds to millions of adenosine (A)-to-inosine (I) modifications are present in eukaryotic transcriptomes and play an essential role in the creation of proteomic and phenotypic diversity. As adenosine and inosine have different base-pairing properties, the functional consequences of these modifications or 'edits' include altering coding potential, splicing, and miRNA-mediated gene silencing of transcripts. However, rather than serving as a static control of gene expression, A-to-I editing provides a means to dynamically rewire the genetic code during development and in a cell-type specific manner. Interestingly, during normal development, in specific cells, and in both neuropathological diseases and cancers, the extent of RNA editing does not directly correlate with levels of the substrate mRNA or the adenosine deaminase that act on RNA (ADAR) editing enzymes, implying that cellular factors are required for spatiotemporal regulation of A-to-I editing. The factors that affect the specificity and extent of ADAR activity have been thoroughly dissected in vitro. Yet, we still lack a complete understanding of how specific ADAR family members can selectively deaminate certain adenosines while others cannot. Additionally, in the cellular environment, ADAR specificity and editing efficiency is likely to be influenced by cellular factors, which is currently an area of intense investigation. Data from many groups have suggested two main mechanisms for controlling A-to-I editing in the cell: (1) regulating ADAR accessibility to target RNAs and (2) protein-protein interactions that directly alter ADAR enzymatic activity. Recent studies suggest cis- and trans-acting RNA elements, heterodimerization and RNA-binding proteins play important roles in regulating RNA editing levels in vivo. WIREs RNA 2016, 7:113-127. doi: 10.1002/wrna.1319. PMID:26612708

  16. CTD Writing and Editing Standards

    SciTech Connect

    Caruthers, C.M.

    1991-03-01

    The Computer and Telecommunication Division (CTD) recognizes that the communication of clear, accurate, reasonably complete information is essential to the success of its Laboratory mission. CTD therefore encourages all Division personnel to adhere to the principles of good writing and to the standards for grammar, usage, style, formats, and publication procedures that are described in CTD Writing and Editing Standards. We encourage CTD personnel to read CTD Writing and Editing Standards and to use it continually as a desktop reference. It will help CTD writers to produce better documents consistent with CTD standards in less time. Applying the principles specified in this document on how to write and organize technical information will speed up the editing, review, and revision processes. CTD Writing and Editing Standards complements the Argonne National Laboratory Technical Publications Guide, which serves as the basic Argonne documentation reference on issues concerning DOE orders and guidelines, NRC directives, and other sponsor requirements. However, this Laboratory-wide document does not address matters of grammar or style. Documents recommended in CTD Writing and Editing Standards are usually available for purchase at the Document Distribution Counter (Building 221, Room A-134) or through the mail (by calling extension 2-5405 and ordering copies).

  17. Distortion-free {sup 13}C NMR spectroscopy in coal: {sup 1}H rotating-frame dynamic nuclear polarization and {sup 1}H-{sup 13}C cross-polarization

    SciTech Connect

    Wind, R.A.

    1993-12-31

    A {sup 1}H-{sup 13}C cross-polarization (CP) experiment is described in which the {sup 1}H magnetization, used in CP, is obtained via dynamic nuclear polarization (DNP) in the proton rotating frame (RF DNP). This experiment can be carried out in coal and other solids containing unpaired electrons. In this so-called RF DNP-CP experiment, interplay effects between the {sup 1}H-{sup 13}C polarization-transfer times and the {sup 1}H rotating-frame relaxation time are avoided; thus {sup 13}C spectral distortions due to these effects are prevented. Moreover, multiple-contact RF DNP-CP experiments are possible, and these experiments reduce the measuring time of a {sup 13}C spectrum. An application of the RF DNP-CP technique in a low-volatile bituminous coal is given. 25 refs., 3 figs.

  18. A study of conformational stability of poly(L-alanine), poly(L-valine), and poly(L-alanine)/poly(L-valine) blends in the solid state by (13)C cross-polarization/magic angle spinning NMR.

    PubMed

    Murata, Katsuyoshi; Kuroki, Shigeki; Kimura, Hideaki; Ando, Isao

    2002-06-01

    13C cross-polarization/magic angle spinning (CP/MAS) NMR and (1)H T(1rho) experiments of poly(L-alanine) (PLA), poly(L-valine) (PLV), and PLA/PLV blends have been carried out in order to elucidate the conformational stability of the polypeptides in the solid state. These were prepared by adding a trifluoroacetic acid (TFA) solution of the polymer with a 2.0 wt/wt % of sulfuric acid (H(2)SO(4)) to alkaline water. From these experimental results, it is clarified that the conformations of PLA and PLV in their blends are strongly influenced by intermolecular hydrogen-bonding interactions that cause their miscibility at the molecular level. PMID:11948439

  19. Understanding Editing Behaviors in Multilingual Wikipedia.

    PubMed

    Kim, Suin; Park, Sungjoon; Hale, Scott A; Kim, Sooyoung; Byun, Jeongmin; Oh, Alice H

    2016-01-01

    Multilingualism is common offline, but we have a more limited understanding of the ways multilingualism is displayed online and the roles that multilinguals play in the spread of content between speakers of different languages. We take a computational approach to studying multilingualism using one of the largest user-generated content platforms, Wikipedia. We study multilingualism by collecting and analyzing a large dataset of the content written by multilingual editors of the English, German, and Spanish editions of Wikipedia. This dataset contains over two million paragraphs edited by over 15,000 multilingual users from July 8 to August 9, 2013. We analyze these multilingual editors in terms of their engagement, interests, and language proficiency in their primary and non-primary (secondary) languages and find that the English edition of Wikipedia displays different dynamics from the Spanish and German editions. Users primarily editing the Spanish and German editions make more complex edits than users who edit these editions as a second language. In contrast, users editing the English edition as a second language make edits that are just as complex as the edits by users who primarily edit the English edition. In this way, English serves a special role bringing together content written by multilinguals from many language editions. Nonetheless, language remains a formidable hurdle to the spread of content: we find evidence for a complexity barrier whereby editors are less likely to edit complex content in a second language. In addition, we find that multilinguals are less engaged and show lower levels of language proficiency in their second languages. We also examine the topical interests of multilingual editors and find that there is no significant difference between primary and non-primary editors in each language. PMID:27171158

  20. Understanding Editing Behaviors in Multilingual Wikipedia

    PubMed Central

    Hale, Scott A.; Kim, Sooyoung; Byun, Jeongmin; Oh, Alice H.

    2016-01-01

    Multilingualism is common offline, but we have a more limited understanding of the ways multilingualism is displayed online and the roles that multilinguals play in the spread of content between speakers of different languages. We take a computational approach to studying multilingualism using one of the largest user-generated content platforms, Wikipedia. We study multilingualism by collecting and analyzing a large dataset of the content written by multilingual editors of the English, German, and Spanish editions of Wikipedia. This dataset contains over two million paragraphs edited by over 15,000 multilingual users from July 8 to August 9, 2013. We analyze these multilingual editors in terms of their engagement, interests, and language proficiency in their primary and non-primary (secondary) languages and find that the English edition of Wikipedia displays different dynamics from the Spanish and German editions. Users primarily editing the Spanish and German editions make more complex edits than users who edit these editions as a second language. In contrast, users editing the English edition as a second language make edits that are just as complex as the edits by users who primarily edit the English edition. In this way, English serves a special role bringing together content written by multilinguals from many language editions. Nonetheless, language remains a formidable hurdle to the spread of content: we find evidence for a complexity barrier whereby editors are less likely to edit complex content in a second language. In addition, we find that multilinguals are less engaged and show lower levels of language proficiency in their second languages. We also examine the topical interests of multilingual editors and find that there is no significant difference between primary and non-primary editors in each language. PMID:27171158

  1. A structural determinant required for RNA editing

    PubMed Central

    Tian, Nan; Yang, Yun; Sachsenmaier, Nora; Muggenhumer, Dominik; Bi, Jingpei; Waldsich, Christina; Jantsch, Michael F.; Jin, Yongfeng

    2011-01-01

    RNA editing by adenosine deaminases acting on RNAs (ADARs) can be both specific and non-specific, depending on the substrate. Specific editing of particular adenosines may depend on the overall sequence and structural context. However, the detailed mechanisms underlying these preferences are not fully understood. Here, we show that duplex structures mimicking an editing site in the Gabra3 pre-mRNA unexpectedly fail to support RNA editing at the Gabra3 I/M site, although phylogenetic analysis suggest an evolutionarily conserved duplex structure essential for efficient RNA editing. These unusual results led us to revisit the structural requirement for this editing by mutagenesis analysis. In vivo nuclear injection experiments of mutated editing substrates demonstrate that a non-conserved structure is a determinant for editing. This structure contains bulges either on the same or the strand opposing the edited adenosine. The position of these bulges and the distance to the edited base regulate editing. Moreover, elevated folding temperature can lead to a switch in RNA editing suggesting an RNA structural change. Our results indicate the importance of RNA tertiary structure in determining RNA editing. PMID:21427087

  2. Gas Metal Arc Welding and Flux-Cored Arc Welding. Third Edition. Teacher Edition [and] Student Edition [and] Student Workbook.

    ERIC Educational Resources Information Center

    Knapp, John; Harper, Eddie

    This packet, containing a teacher's edition, a student edition, and a student workbook, introduces students to high deposition welding and processes for "shielding" a weld. In addition to general information, the teacher edition consists of introductory pages and teacher pages, as well as unit information that corresponds to the materials in the…

  3. Gas Tungsten Arc Welding and Plasma Arc Cutting. Teacher Edition [and] Student Edition [and] Student Workbook. Second Edition.

    ERIC Educational Resources Information Center

    Harper, Eddie; Knapp, John

    This packet of instructional materials for a gas tungsten arc welding (GTAW) and plasma arc cutting course is comprised of a teacher edition, student edition, and student workbook. The teacher edition consists of introductory pages and teacher pages. Introductory pages include training and competency profile, state duty/task crosswalk,…

  4. Genome editing comes of age.

    PubMed

    Kim, Jin-Soo

    2016-09-01

    Genome editing harnesses programmable nucleases to cut and paste genetic information in a targeted manner in living cells and organisms. Here, I review the development of programmable nucleases, including zinc finger nucleases (ZFNs), TAL (transcription-activator-like) effector nucleases (TALENs) and CRISPR (cluster of regularly interspaced palindromic repeats)-Cas9 (CRISPR-associated protein 9) RNA-guided endonucleases (RGENs). I specifically highlight the key advances that set the foundation for the rapid and widespread implementation of CRISPR-Cas9 genome editing approaches that has revolutionized the field. PMID:27490630

  5. Language Editing at Astronomy & Astrophysics

    NASA Astrophysics Data System (ADS)

    Adams, J.

    2011-07-01

    In 2002, the A&A Board of Directors voted that all articles must be written in English and decided to improve the overall quality of the language in the articles with the help of a team of language editors. This article reviews the general advantages of editing the English expression and describes both the aims of this effort and its place in the full publication process. This is followed by the Guide to language editing that has been available on the Journal's website for several years now.

  6. Concepts of genetics: II edition

    SciTech Connect

    Klug, W.S.; Cummings, M.R.

    1986-01-01

    This book provides an introduction to the molecule, and progresses logically through cellular genetics and the genetics of organisms to the larger picture of population genetics. The Second Edition features new chapters on quantitative inheritance and recombinant DNA, a new appendix with a human gene map and coverage of gene disorders, expanded coverage of bacterial and viral genetics, and consolidated coverage of sex linkage, sex determination, sex chromosome abberations, and sex differentiation. Dozens of new figures are added in this edition. All diagrams, photographs, and tables work hand-in-hand with the text to explain important concepts. Practical exercises with answers at the back of the text provide immediate feedback.

  7. [An introduction on the editions of Zhang Gao's Yi shuo (Medical Narrations)].

    PubMed

    Wang, Xuguang; Lu, Xiang

    2014-11-01

    Zhang Gao's Yi shuo (Medical Narrations) of the Southern Song Dynasty had 2 kinds of editions: domestic editions and foreign editions. The former includes 1 Song edition, 14 Ming editions, 3 Qing editions and 25 editions after the Republic of China. The latter, mainly 2 classes, the Japanese edition and Korean printing type edition. In the Ming Dynasty, the editions of Yi shuo generated 2 branches: inherited edition and supplementary edition. The inherited editions include Gu Dingfang's edition, Zhang Yaode's edition, Wu Mianxue's edition, Wu Zhongheng's edition, Wang Kentang's edition, editions from Si ku quan shu (Imperial Collection of Four), stereotype edition of the 3th year of Xuantong reign (1911) from Shanghai Civilization Bookstore, edition of the 2(nd) year of Manji of Japan etc. The supplemental editions include Zhang Zili's edition, Shen Fan's edition, Fu Feng'ao's edition, transcript of the late Ming Dynasty preserved in the Library of Peking University, and Korean printing type edition etc. PMID:25620361

  8. Power Product Equipment Technician: Equipment Systems. Teacher Edition. Student Edition.

    ERIC Educational Resources Information Center

    Hilley, Robert

    This packet contains teacher and student editions on the topic of equipment systems, intended for the preparation of power product equipment technicians. This publication contains seven units: (1) principles of power transmission; (2) mechanical drive systems; (3) principles of fluid power; (4) hydraulic and pneumatic drive systems; (5) wheel and…

  9. Shielded Metal Arc Pipe Welding. Teacher Edition. Second Edition.

    ERIC Educational Resources Information Center

    Fortney, Clarence; And Others

    This second edition of the shielded metal arc pipe welding curriculum guide presents both basic and advanced pipe welding skills. All specifications for procedure and welder qualification are presented according to national standards. The standards also include the test position for both groove and fillet pipe welding. The guide contains three…

  10. Civil Technology Applications. Teacher Edition [and] Student Edition.

    ERIC Educational Resources Information Center

    Schertz, Karen

    Teacher and student editions of Civil Technology Applications are one in a series of competency-based instructional materials for drafting and civil technology programs. It includes the technical content and tasks necessary for a student to be employed as a drafter or civil technician in a civil engineering firm. Introductory pages in the teacher…

  11. Controlled flexibility in technical editing - The levels-of-edit concept at JPL

    NASA Technical Reports Server (NTRS)

    Buehler, M. F.

    1977-01-01

    The levels-of-edit concept, which can be used to specify the amount of editorial effort involved in the preparation of a manuscript for publication, is discussed. Nine types of editing are identified and described. These include coordination edit (preparing estimates, gathering cost data, monitoring production processes), policy edit, integrity edit (making sure that parts of a publication match in a physical or numerical sense), screening edit (ensuring that the quality of camera-ready copy is sufficient for external publication), copy clarification edit, format edit, mechanical style edit, language edit, and substantive edit (reviewing the manuscript for content coherence, emphasis, subordination and parallelism). These functions are grouped into five levels of edit. An edit-level number is assigned to each manuscript, providing a quantitative and qualitative indicator of the editing to be done which is clearly understood by authors, managers, and editors alike. In addition, clear boundaries are drawn between normal and extraordinary editing tasks. Individual organizations will group various edits in different ways to reflect their needs and priorities; the essential element of the system is unambiguous definition and coding of the types and amount of work to be done.

  12. Strategies of Qualitative Inquiry. Third Edition

    ERIC Educational Resources Information Center

    Denzin, Norman K., Ed.; Lincoln, Yvonna S., Ed.

    2007-01-01

    "Strategies of Qualitative Inquiry, Third Edition," the second volume in the paperback version of "The SAGE Handbook of Qualitative Research, 3rd Edition," consists of Part III of the handbook ("Strategies of Inquiry"). "Strategies of Qualitative Inquiry, Third Edition" presents the major tactics--historically, the research methods--that…

  13. 48 CFR 53.102 - Current editions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Current editions. 53.102... AND FORMS FORMS General 53.102 Current editions. The form prescriptions in subpart 53.2 and the illustrations in subpart 53.3 contain current edition dates. Contracting officers shall use the current...

  14. 48 CFR 53.102 - Current editions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 2 2014-10-01 2014-10-01 false Current editions. 53.102... AND FORMS FORMS General 53.102 Current editions. The form prescriptions in subpart 53.2 and the illustrations in subpart 53.3 contain current edition dates. Contracting officers shall use the current...

  15. 48 CFR 53.102 - Current editions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false Current editions. 53.102... AND FORMS FORMS General 53.102 Current editions. The form prescriptions in subpart 53.2 and the illustrations in subpart 53.3 contain current edition dates. Contracting officers shall use the current...

  16. 48 CFR 53.102 - Current editions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 2 2013-10-01 2013-10-01 false Current editions. 53.102... AND FORMS FORMS General 53.102 Current editions. The form prescriptions in subpart 53.2 and the illustrations in subpart 53.3 contain current edition dates. Contracting officers shall use the current...

  17. 48 CFR 53.102 - Current editions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 2 2011-10-01 2011-10-01 false Current editions. 53.102... AND FORMS FORMS General 53.102 Current editions. The form prescriptions in subpart 53.2 and the illustrations in subpart 53.3 contain current edition dates. Contracting officers shall use the current...

  18. Strengthening Family Resilience, Second Edition

    ERIC Educational Resources Information Center

    Walsh, Froma

    2006-01-01

    In a fully revised, updated, and expanded second edition, this informative clinical resource and text presents Froma Walsh's family resilience framework for intervention and prevention with clients dealing with adversity. Drawing on extensive research and clinical experience, the author describes key processes in resilience for practitioners to…

  19. Teaching Population Concepts. Revised Edition.

    ERIC Educational Resources Information Center

    King, Pat; Landahl, John

    This edition is designed to help teachers provide their students with some basic population concepts with stress placed on the elements of decision making. In the first section of the pamphlet, some of the basic concepts of population study are presented. These include populations, growth rates, birth and death rates, doubling time, migration, age…

  20. Veterinary Microbiology, 3rd Edition

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Veterinary Microbiology, Third Edition is organized into four sections and begins with an updated and expanded introductory section on infectious disease pathogenesis, diagnosis and clinical management. The second section covers bacterial and fungal pathogens, and the third section describes viral d...

  1. Nuclear Electricity. 5th Edition.

    ERIC Educational Resources Information Center

    Hore-Lacy, Ian

    Educators must address the need for young people to be informed about both the scientific concepts and the reasons for controversy when dealing with controversial issues. Young people must be given the opportunity to form their own opinions when presented with evidence for conflicting arguments. Previous editions of "Nuclear Electricity" have…

  2. The Horizon Report. 2007 Edition

    ERIC Educational Resources Information Center

    New Media Consortium, 2007

    2007-01-01

    This fourth edition of the New Media Consortium's (NMC) annual "Horizon Report" describes the continuing work of the Horizon Project, a research-oriented effort that seeks to identify and describe emerging technologies likely to have a large impact on teaching, learning, or creative expression within higher education. Drawing on ongoing…

  3. Biology Teachers' Handbook, Second Edition.

    ERIC Educational Resources Information Center

    Klinckmann, Evelyn; And Others

    This is the second edition of the handbook written for teachers using the Biological Sciences Curriculum Study (BSCS) materials. Section one describes the historical background, aims and emphases of the BSCS materials and includes behavioral objectives for teaching inquiry processes. Section two includes 44 "Invitations to Enquiry." Section three,…

  4. A Writer's Reference. Third Edition.

    ERIC Educational Resources Information Center

    Hacker, Diana

    Designed to save the user time and packaged in a compact size which lies flat, this book is easy to consult while revising and editing a written draft. The book's "main menu," just inside the front cover, displays the contents as briefly and simply as possible. Each of the 12 sections in the book's main menu leads the user to a tabbed divider, on…

  5. Teaching Reading Sourcebook, Second Edition

    ERIC Educational Resources Information Center

    Honig, Bill; Diamond, Linda; Gutlohn, Linda

    2008-01-01

    The "Teaching Reading Sourcebook, Second Edition" is a comprehensive reference about reading instruction. Organized according to the elements of explicit instruction (what? why? when? and how?), the "Sourcebook" includes both a research-informed knowledge base and practical sample lesson models. It teaches the key elements of an effective reading…

  6. The Foundation Directory, Edition 4.

    ERIC Educational Resources Information Center

    Lewis, Marianna O., Ed.; Bowers, Patricia, Ed.

    The fourth edition of "The Foundation Directory" lists and describes 5,454 foundations and surveys their grants. The directory was prepared from foundation reports and government records. The foundations listed either have assets of $500.00 or made grants totally at least $25,000.00 in the year of record. Education is the leading beneficiary of…

  7. Promoting School Success. Third Edition

    ERIC Educational Resources Information Center

    Lovitt, Thomas C.

    2007-01-01

    Like its two predecessors, "Preventing School Dropouts" [C1991] and "Preventing School Failure" [C2000], this third edition is a book about teaching. Although primarily written for teachers, tutors and parents may also find this book helpful. It is a collection of carefully selected teaching techniques aimed at helping young adults learn important…

  8. Resident Care Guide. Third Edition.

    ERIC Educational Resources Information Center

    Woodbridge State School, NJ.

    The third edition of the Woodbridge State School Cottage Life Department Resident Care Guide is explained to be a developmental status scale devised in 1969 as part of a 5-year study for the purposes of measuring the entire population's self-help training abilities. The department is said to serve 954 residents; 424 are non-ambulatory and 530 are…

  9. Modern Indian Psychology. Revised Edition.

    ERIC Educational Resources Information Center

    Bryde, John F.

    Written on the basis of senior Indian verbal relatings collected over a 23-year span, this revised edition on modern Indian psychology incorporates suggestions from Indian students and their teachers, Indian and non-Indian social studies experts, and other Indian people. The book contains 6 major divisions: (1) "Culture and Indian Values" relates…

  10. Money and Schools. Fifth Edition

    ERIC Educational Resources Information Center

    Thompson, David C.; Crampton, Faith E.; Wood, R. Craig

    2012-01-01

    In the new edition of this essential, all-inclusive text, the authors provide more important research for future principals and others enrolled in graduate-level school finance courses. Written in a style that is highly readable, the book offers strong connections to real-world experiences. Readers get both a broad overview of funding concepts and…

  11. Unlocking Mathematics Teaching. Second Edition

    ERIC Educational Resources Information Center

    Koshy, Valsa, Ed.; Murray, Jean, Ed.

    2011-01-01

    Now in a fully updated second edition, "Unlocking Mathematics Teaching" is a comprehensive guide to teaching mathematics in the primary school. Combining theory and practice, selected experts outline the current context of mathematics education. They suggest strategies, activities and examples to help develop readers understanding and confidence…

  12. The Horizon Report. 2006 Edition

    ERIC Educational Resources Information Center

    New Media Consortium, 2006

    2006-01-01

    This third edition of the New Media Consortium's (NMC) annual "Horizon Report" describes the continuing work of the Horizon Project, a research-oriented effort that seeks to identify and describe emerging technologies likely to have a large impact on teaching, learning, or creative expression within higher education. Drawing on ongoing discussions…

  13. Writing Back: Revising and Editing.

    ERIC Educational Resources Information Center

    Johnstone, Velerie M.

    1990-01-01

    Discusses a "write-back" strategy for use when there is insufficient class time for conferencing with peers or the teacher. Notes that the teacher agrees to respond to students' drafts within 24 hours. Discusses 3 options available to students: (1) write back/respond; (2) edit; and (3) writing back. (RS)

  14. The Horizon Report. 2004 Edition

    ERIC Educational Resources Information Center

    New Media Consortium, 2004

    2004-01-01

    This first edition of the New Media Consortium's (NMC) annual "Horizon Report" details findings of the Horizon Project, a research-oriented effort that seeks to identify and describe emerging technologies likely to have a large impact on teaching, learning, or creative expression within higher education. Drawing on an ongoing series of interviews…

  15. The Horizon Report. 2005 Edition

    ERIC Educational Resources Information Center

    New Media Consortium, 2005

    2005-01-01

    This second edition of the New Media Consortium's (NMC) annual "Horizon Report" describes the continuing work of the Horizon Project, a research-oriented effort that seeks to identify and describe emerging technologies likely to have a large impact on teaching, learning, or creative expression within higher education. Drawing on an ongoing series…

  16. Are substitution rates and RNA editing correlated?

    PubMed Central

    2010-01-01

    Background RNA editing is a post-transcriptional process that, in seed plants, involves a cytosine to uracil change in messenger RNA, causing the translated protein to differ from that predicted by the DNA sequence. RNA editing occurs extensively in plant mitochondria, but large differences in editing frequencies are found in some groups. The underlying processes responsible for the distribution of edited sites are largely unknown, but gene function, substitution rate, and gene conversion have been proposed to influence editing frequencies. Results We studied five mitochondrial genes in the monocot order Alismatales, all showing marked differences in editing frequencies among taxa. A general tendency to lose edited sites was observed in all taxa, but this tendency was particularly strong in two clades, with most of the edited sites lost in parallel in two different areas of the phylogeny. This pattern is observed in at least four of the five genes analyzed. Except in the groups that show an unusually low editing frequency, the rate of C-to-T changes in edited sites was not significantly higher that in non-edited 3rd codon positions. This may indicate that selection is not actively removing edited sites in nine of the 12 families of the core Alismatales. In all genes but ccmB, a significant correlation was found between frequency of change in edited sites and synonymous substitution rate. In general, taxa with higher substitution rates tend to have fewer edited sites, as indicated by the phylogenetically independent correlation analyses. The elimination of edited sites in groups that lack or have reduced levels of editing could be a result of gene conversion involving a cDNA copy (retroprocessing). If so, this phenomenon could be relatively common in the Alismatales, and may have affected some groups recurrently. Indirect evidence of retroprocessing without a necessary correlation with substitution rate was found mostly in families Alismataceae and Hydrocharitaceae

  17. Diesel Technology: Steering and Suspension. Second Edition. Teacher Edition [and] Student Edition.

    ERIC Educational Resources Information Center

    Miller, Roger; Scarberry, Terry; Tesch, Carl; Kellum, Mary

    These teacher and student editions on steering and suspension are part of the diesel mechanics series of instructional materials. The series aligns with the medium/heavy duty truck task list developed by the National Automotive Technicians Education Foundation and used by the National Institute for Automotive Service Excellence in the…

  18. Diesel Technology: Safety Skills. Teacher Edition [and] Student Edition. Second Edition.

    ERIC Educational Resources Information Center

    Kellum, Mary

    Teacher and student editions of this document are one in a series of competency-based instructional materials for diesel technology programs. The series aligns with the medium/heavy diesel duty truck task list used by the National Institute for Automotive Service Excellence in the certification of medium/heavy duty truck technicians. Introductory…

  19. Statistical Physics Approaches to RNA Editing

    NASA Astrophysics Data System (ADS)

    Bundschuh, Ralf

    2012-02-01

    The central dogma of molecular Biology states that DNA is transcribed base by base into RNA which is in turn translated into proteins. However, some organisms edit their RNA before translation by inserting, deleting, or substituting individual or short stretches of bases. In many instances the mechanisms by which an organism recognizes the positions at which to edit or by which it performs the actual editing are unknown. One model system that stands out by its very high rate of on average one out of 25 bases being edited are the Myxomycetes, a class of slime molds. In this talk we will show how the computational methods and concepts from statistical Physics can be used to analyze DNA and protein sequence data to predict editing sites in these slime molds and to guide experiments that identified previously unknown types of editing as well as the complete set of editing events in the slime mold Physarum polycephalum.

  20. Spectrally edited 2D 13Csbnd 13C NMR spectra without diagonal ridge for characterizing 13C-enriched low-temperature carbon materials

    NASA Astrophysics Data System (ADS)

    Johnson, Robert L.; Anderson, Jason M.; Shanks, Brent H.; Fang, Xiaowen; Hong, Mei; Schmidt-Rohr, Klaus

    2013-09-01

    Two robust combinations of spectral editing techniques with 2D 13Csbnd 13C NMR have been developed for characterizing the aromatic components of 13C-enriched low-temperature carbon materials. One method (exchange with protonated and nonprotonated spectral editing, EXPANSE) selects cross peaks of protonated and nearby nonprotonated carbons, while the other technique, dipolar-dephased double-quantum/single-quantum (DQ/SQ) NMR, selects signals of bonded nonprotonated carbons. Both spectra are free of a diagonal ridge, which has many advantages: Cross peaks on the diagonal or of small intensity can be detected, and residual spinning sidebands or truncation artifacts associated with the diagonal ridge are avoided. In the DQ/SQ experiment, dipolar dephasing of the double-quantum coherence removes protonated-carbon signals; this approach also eliminates the need for high-power proton decoupling. The initial magnetization is generated with minimal fluctuation by combining direct polarization, cross polarization, and equilibration by 13C spin diffusion. The dipolar dephased DQ/SQ spectrum shows signals from all linkages between aromatic rings, including a distinctive peak from polycondensed aromatics. In EXPANSE NMR, signals of protonated carbons are selected in the first spectral dimension by short cross polarization combined with dipolar dephasing difference. This removes ambiguities of peak assignment to overlapping signals of nonprotonated and protonated aromatic carbons, e.g. near 125 ppm. Spin diffusion is enhanced by dipolar-assisted rotational resonance. Before detection, Csbnd H dipolar dephasing by gated decoupling is applied, which selects signals of nonprotonated carbons. Thus, only cross peaks due to magnetization originating from protonated C and ending on nearby nonprotonated C are retained. Combined with the chemical shifts deduced from the cross-peak position, this double spectral editing defines the bonding environment of aromatic, COO, and Cdbnd O carbons

  1. The evolution of chloroplast RNA editing.

    PubMed

    Tillich, Michael; Lehwark, Pascal; Morton, Brian R; Maier, Uwe G

    2006-10-01

    RNA editing alters the nucleotide sequence of an RNA molecule so that it deviates from the sequence of its DNA template. Different RNA-editing systems are found in the major eukaryotic lineages, and these systems are thought to have evolved independently. In this study, we provide a detailed analysis of data on C-to-U editing sites in land plant chloroplasts and propose a model for the evolution of RNA editing in land plants. First, our data suggest that the limited RNA-editing system of seed plants and the much more extensive systems found in hornworts and ferns are of monophyletic origin. Further, although some eukaryotic editing systems appear to have evolved to regulate gene expression, or at least are now involved in gene regulation, there is no evidence that RNA editing plays a role in gene regulation in land plant chloroplasts. Instead, our results suggest that land plant chloroplast C-to-U RNA editing originated as a mechanism to generate variation at the RNA level, which could complement variation at the DNA level. Under this model, many of the original sites, particularly in seed plants, have been subsequently lost due to mutation at the DNA level, and the function of extant sites is merely to conserve certain codons. This is the first comprehensive model for the evolution of the chloroplast RNA-editing system of land plants and may also be applicable to the evolution of RNA editing in plant mitochondria. PMID:16835291

  2. Stochastic molecular motions in the nematic, smectic-A, and solid phases of p,p{sup '}-di-n-heptyl-azoxybenzene as seen by quasielastic neutron scattering and {sup 13}C cross-polarization magic-angle-spinning NMR

    SciTech Connect

    ZajaPc, Wojciech; Urban, Stanislaw; Domenici, Valentina; Geppi, Marco; Veracini, Carlo Alberto; Telling, Mark T. F.; Gabrys, Barbara J.

    2006-05-15

    Molecular rotational dynamics in p,p{sup '}-di-n-heptyl-azoxybenzene was studied by means of quasielastic neutron scattering (QENS) and {sup 13}C cross-polarization magic-angle-spinning (CPMAS) NMR. Fast reorientation of the hydrogen nuclei was observed by QENS in the two liquid crystalline (LC) phases nematic and smectic A, as well as in the crystalline phase. The latter could not be restricted to the -CH{sub 3} rotations alone, and a clear indication was found of some other reorientation motions persisting in the crystal. Two Lorentz-type components convoluted with the resolution function gave an excellent fit to the QENS spectra in both LC phases. The narrow (slow) component was attributed to the reorientation of the whole molecule around the long axis. The corresponding characteristic time of {approx}130 ps agreed well with the values obtained in recent dielectric relaxation and {sup 2}H NMR studies. The full width at half maximum of the broader (fast) component shows a quadratic Q dependence (Q is the momentum transfer). Hence the corresponding motions could be described by a stretched exponential correlation function and were interpreted as various ''crankshaft-type'' motions within the alkyl tails. The {sup 13}C CPMAS experiments fully corroborated the QENS results, sometimes considered ambiguous in complex systems.

  3. Stochastic molecular motions in the nematic, smectic-A, and solid phases of p,p'-di-n-heptyl-azoxybenzene as seen by quasielastic neutron scattering and 13C cross-polarization magic-angle-spinning NMR.

    PubMed

    Zajac, Wojciech; Urban, Stanisław; Domenici, Valentina; Geppi, Marco; Veracini, Carlo Alberto; Telling, Mark T F; Gabryś, Barbara J

    2006-05-01

    Molecular rotational dynamics in p,p'-di-n-heptyl-azoxybenzene was studied by means of quasielastic neutron scattering (QENS) and 13C cross-polarization magic-angle-spinning (CPMAS) NMR. Fast reorientation of the hydrogen nuclei was observed by QENS in the two liquid crystalline (LC) phases nematic and smectic A, as well as in the crystalline phase. The latter could not be restricted to the -CH3 rotations alone, and a clear indication was found of some other reorientation motions persisting in the crystal. Two Lorentz-type components convoluted with the resolution function gave an excellent fit to the QENS spectra in both LC phases. The narrow (slow) component was attributed to the reorientation of the whole molecule around the long axis. The corresponding characteristic time of approximately 130 ps agreed well with the values obtained in recent dielectric relaxation and 2H NMR studies. The full width at half maximum of the broader (fast) component shows a quadratic Q dependence (Q is the momentum transfer). Hence the corresponding motions could be described by a stretched exponential correlation function and were interpreted as various "crankshaft-type" motions within the alkyl tails. The 13C CPMAS experiments fully corroborated the QENS results, sometimes considered ambiguous in complex systems. PMID:16802951

  4. An agent based model of genotype editing

    SciTech Connect

    Rocha, L. M.; Huang, C. F.

    2004-01-01

    This paper presents our investigation on an agent-based model of Genotype Editing. This model is based on several characteristics that are gleaned from the RNA editing system as observed in several organisms. The incorporation of editing mechanisms in an evolutionary agent-based model provides a means for evolving agents with heterogenous post-transcriptional processes. The study of this agent-based genotype-editing model has shed some light into the evolutionary implications of RNA editing as well as established an advantageous evolutionary computation algorithm for machine learning. We expect that our proposed model may both facilitate determining the evolutionary role of RNA editing in biology, and advance the current state of research in agent-based optimization.

  5. Image Editing Via Searching Source Image

    NASA Astrophysics Data System (ADS)

    Yu, Han; Deng, Liang-Jian

    Image editing has important applications by changing the image texture, illumination, target location, etc. As an important application of Poisson equation, Poisson image editing processes images on the gradient domain and has been applied to seamless clone, selection editing, image denoising, etc. In this paper, we present a new application of Poisson image editing, which is based on searching source image. The main feature of the new application is all modifying information comes from the source image. Experimental results show that the proposed application performs well.

  6. Fuel Cell Handbook, Fourth Edition

    SciTech Connect

    Stauffer, D.B; Hirschenhofer, J.H.; Klett, M.G.; Engleman, R.R.

    1998-11-01

    Robust progress has been made in fuel cell technology since the previous edition of the Fuel Cell Handbook was published in January 1994. This Handbook provides a foundation in fuel cells for persons wanting a better understanding of the technology, its benefits, and the systems issues that influence its application. Trends in technology are discussed, including next-generation concepts that promise ultra high efficiency and low cost, while providing exceptionally clean power plant systems. Section 1 summarizes fuel cell progress since the last edition and includes existing power plant nameplate data. Section 2 addresses the thermodynamics of fuel cells to provide an understanding of fuel cell operation at two levels (basic and advanced). Sections 3 through 6 describe the four major fuel cell types and their performance based on cell operating conditions. The section on polymer electrolyte membrane fuel cells has been added to reflect their emergence as a significant fuel cell technology. Phosphoric acid, molten carbonate, and solid oxide fuel cell technology description sections have been updated from the previous edition. New information indicates that manufacturers have stayed with proven cell designs, focusing instead on advancing the system surrounding the fuel cell to lower life cycle costs. Section 7, Fuel Cell Systems, has been significantly revised to characterize near-term and next-generation fuel cell power plant systems at a conceptual level of detail. Section 8 provides examples of practical fuel cell system calculations. A list of fuel cell URLs is included in the Appendix. A new index assists the reader in locating specific information quickly.

  7. Pediatric surgical pathology. Second edition

    SciTech Connect

    Dehner, L.P.

    1987-01-01

    The edition provides view of congenital, hereditary, infectious, and inflammatory neoplastic diseases occurring during the first two decades of life, with special reference to clinical, laboratory, and roentgenographic features. Material includes observations from some of the major national studies on Wilms' tumor and rhabdomyosarcomas, the new classification of pediatric malignant lymphomas, a discussion of the role of immunocytochemistry as it applies to the diagnosis of childhood infections and neoplasms, an examination of graft-versus-host disease in the liver and intestinal tract and more.

  8. NASA Pocket Statistics: 1997 Edition

    NASA Technical Reports Server (NTRS)

    1997-01-01

    POCKET STATISTICS is published by the NATIONAL AERONAUTICS AND SPACE ADMINISTRATION (NASA). Included in each edition is Administrative and Organizational information, summaries of Space Flight Activity including the NASA Major Launch Record, Aeronautics and Space Transportation and NASA Procurement, Financial and Workforce data. The NASA Major Launch Record includes all launches of Scout class and larger vehicles. Vehicle and spacecraft development flights are also included in the Major Launch Record. Shuttle missions are counted as one launch and one payload, where free flying payloads are not involved. All Satellites deployed from the cargo bay of the Shuttle and placed in a separate orbit or trajectory are counted as an additional payload.

  9. The Technology of Human Editing (The Friendly Editor).

    ERIC Educational Resources Information Center

    Bush, Don

    1992-01-01

    Contrasts robotic editing with human editing (discussing descriptive grammar, periodic sentences, theme-rheme concept, right-branching, zeugma, and Irish bulls). Maintains that, for any editing that requires thinking, humans are always superior. (SR)

  10. Recovering Microwave Cross-Polarization Losses

    NASA Technical Reports Server (NTRS)

    Seidel, B. L.; Stelzried, C. T.; Ohlson, J. E.

    1985-01-01

    Reception improved by adding normally discarded portion of signal. For signal enhancement, major and minor signals combined in slaved closedloop receiver channels. Both receiver channels served by common local oscillator controlled by phase-locked loop in main channel. 10-MHz intermediate-frequency (IF) signals of channels coherently summed. For polarization tracking, phasemeter added to measure phase difference between points A and B. With new circuit, low-level signal used at receiver to augment main signal.

  11. Study of lignification by noninvasive techniques in growing maize internodes. An investigation by Fourier transform infrared cross-polarization-magic angle spinning 13C-nuclear magnetic resonance spectroscopy and immunocytochemical transmission electron microscopy.

    PubMed

    Joseleau, J P; Ruel, K

    1997-07-01

    Noninvasive techniques were used for the study in situ of lignification in the maturing cell walls of the maize (Zea mays L.) stem. Within the longitudinal axis of a developing internode all of the stages of lignification can be found. The synthesis of the three types of lignins, p-hydroxyphenylpropane (H), guaiacyl (G), and syringyl (S), was investigated in situ by cross-polarization-magic angle spinning 13C-solid-state nuclear magnetic resonance, Fourier transform infrared spectroscopy, and immunocytochemical electron microscopy. The first lignin appearing in the parenchyma is of the G-type preceeding the incorporation of S nuclei in the later stages. However, in vascular bundles, typical absorption bands of S nuclei are visible in the Fourier transform infrared spectra at the earliest stage of lignification. Immunocytochemical determination of the three types of lignin in transmission electron microscopy was possible thanks to the use of antisera prepared against synthetic H, G, and the mixed GS dehydrogenative polymers (K. Ruel, O. Faix, J.P. Joseleau [1994] J Trace Microprobe Tech 12: 247-265). The specificity of the immunological probes demonstrated that there are differences in the relative temporal synthesis of the H, G, and GS lignins in the different tissues undergoing lignification. Considering the intermonomeric linkages predominating in the antigens used for the preparation of the immunological probes, the relative intensities of the labeling obtained provided, for the first time to our knowledge, information about the macromolecular nature of lignins (condensed versus noncondensed) in relation to their ultrastructural localization and development stage. PMID:9232887

  12. The zebrafish genome editing toolkit.

    PubMed

    Ata, H; Clark, K J; Ekker, S C

    2016-01-01

    Zebrafish (Danio rerio) is a unique model organism at the functional intersection between a high fecundity and conserved vertebrate physiology while being amenable to a multitude of genome editing techniques. The genome engineering field has experienced an unprecedented rate of growth in the recent years since the introduction of designer endonucleases, such as zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats-Cas9 systems. With the ever-evolving toolset available to the scientific community, the important question one should ask is not simply how to make a mutant line, but rather how best to do so. For this purpose, understanding the toolset is just one end of the equation; understanding how DNA is repaired once double-strand breaks are induced by designer endonucleases, as well as understanding proper fish handling and line maintenance techniques, are also essential to rapidly edit the zebrafish genome. This chapter is outlined to provide a bird's-eye view on each of these three components. The goal of this chapter is to facilitate the adoption of the zebrafish as a model to study human genetic disease and to rapidly analyze the function of the vertebrate genome. PMID:27443924

  13. Automatic data editing: a brief introduction

    SciTech Connect

    Liepins, G.E.

    1982-01-01

    This paper briefly discusses the automatic data editing process: (1) check the data records for consistency, (2) analyze the inconsistent records to determine the inconsistent variables. It is stated that the application of automatic data editing is broad, and two specific examples are cited. One example, that of a vehicle maintenance data base is used to illustrate the process.

  14. Managing the Incompetent Teacher. Second Edition.

    ERIC Educational Resources Information Center

    Bridges, Edwin M.

    Featuring the same practical guidelines for ridding schools of incompetent teachers as the 1984 edition, this new edition incorporates substantially revised material on three topics: criteria and information sources for evaluating teaching effectiveness, remediation procedures, and grounds for dismissal. The book presents an eight-step systematic,…

  15. Editing Technical Proposals (The Friendly Editor).

    ERIC Educational Resources Information Center

    Bush, Don

    1994-01-01

    Makes suggestions for editing technical proposals. Discusses the marketeers, the hierarchy of hype, how to save days, managing story boards, expediting a laborious process, teaching engineers to write, writing incrementally, the art group, and the editing task. Argues that the best proposals come from starting to write early. (SR)

  16. Sex Differences in Cognitive Abilities. Fourth Edition

    ERIC Educational Resources Information Center

    Halpern, Diane F.

    2011-01-01

    The fourth edition of "Sex Differences in Cognitive Abilities" critically examines the breadth of research on this complex and controversial topic, with the principal aim of helping the reader to understand where sex differences are found--and where they are not. Since the publication of the third edition, there have been many exciting and…

  17. Web Thermo Tables (WTT) - Professional Edition

    National Institute of Standards and Technology Data Gateway

    SRD 203 NIST/TRC Web Thermo Tables (WTT) - Professional Edition (Online Subscription)   WTT - Professional Edition, a Web version of the TRC Thermodynamic Tables, represents a complete collection of critically evaluated thermodynamic property data primarily for pure organic compounds. As of Nov. 2011, WTT contains information on 23999 compounds.

  18. Web Thermo Tables (WTT) - Lite Edition

    National Institute of Standards and Technology Data Gateway

    SRD 202 NIST/TRC Web Thermo Tables (WTT) - Lite Edition (Online Subscription)   WTT - Lite Edition, a Web version of the TRC Thermodynamic Tables, represents a collection of critically evaluated thermodynamic property data for 150 commonly-used (primarily organic) pure compounds.

  19. Novel modes of RNA editing in mitochondria.

    PubMed

    Moreira, Sandrine; Valach, Matus; Aoulad-Aissa, Mohamed; Otto, Christian; Burger, Gertraud

    2016-06-01

    Gene structure and expression in diplonemid mitochondria are unparalleled. Genes are fragmented in pieces (modules) that are separately transcribed, followed by the joining of module transcripts to contiguous RNAs. Some instances of unique uridine insertion RNA editing at module boundaries were noted, but the extent and potential occurrence of other editing types remained unknown. Comparative analysis of deep transcriptome and genome data from Diplonema papillatum mitochondria reveals ∼220 post-transcriptional insertions of uridines, but no insertions of other nucleotides nor deletions. In addition, we detect in total 114 substitutions of cytosine by uridine and adenosine by inosine, amassed into unusually compact clusters. Inosines in transcripts were confirmed experimentally. This is the first report of adenosine-to-inosine editing of mRNAs and ribosomal RNAs in mitochondria. In mRNAs, editing causes mostly amino-acid additions and non-synonymous substitutions; in ribosomal RNAs, it permits formation of canonical secondary structures. Two extensively edited transcripts were compared across four diplonemids. The pattern of uridine-insertion editing is strictly conserved, whereas substitution editing has diverged dramatically, but still rendering diplonemid proteins more similar to other eukaryotic orthologs. We posit that RNA editing not only compensates but also sustains, or even accelerates, ultra-rapid evolution of genome structure and sequence in diplonemid mitochondria. PMID:27001515

  20. Michigan Library Statistical Report. 1997 Edition.

    ERIC Educational Resources Information Center

    Michigan Library, Lansing.

    This 1997 edition focuses on statistical data supplied by Michigan public libraries, public library cooperatives, and those public libraries which serve as regional or subregional outlets for blind and physically handicapped patrons. Statistics on academic libraries are also presented in this edition, and summary statistics for prior fiscal years…

  1. New Edition of Chinese Biochemistry Textbook.

    ERIC Educational Resources Information Center

    Jian-Chuan, Ma

    1988-01-01

    Discusses the four previous editions of the biochemistry medical textbooks called the "Nationwide Unified Textbooks." Notes the new (1989) edition is much smaller, is organized differently, has new material, has a reorganized Dynamic Biochemistry core, and shows great importance to clinical biochemistry. (MVL)

  2. Teaching Strategies for Ethnic Studies. Fifth Edition.

    ERIC Educational Resources Information Center

    Banks, James A.

    A major goal of this fifth edition is to help present and future teachers acquire the knowledge, concepts, strategies, and resources needed to integrate information about ethnic groups into the mainstream curriculum. This edition incorporates new terms and concepts that are emerging in the field of multicultural education. Information on ethnic…

  3. Handbook of Reference Sources. Third Edition.

    ERIC Educational Resources Information Center

    Nichols, Margaret Irby

    This third edition of popular and useful reference works, which emphasizes the needs of small libraries, contains 975 annotated entries and lists 201 additional titles (most with bibliographic and order information) in the annotations, representing an expansion of 30 percent over the second edition. The appendix lists 116 basic or core reference…

  4. Teaching Visually Impaired Children. 3rd Edition

    ERIC Educational Resources Information Center

    Bishop, Virginia E.

    2004-01-01

    In this exceptional new third edition, the author has retained much of the practical "how to" approach of the previous editions, but adds depth in two dimensions: learning theory and the educational process. This book is "so comprehensive in scope and complete in detail that it would be the most likely recommended" (from the foreword by Dr.…

  5. Exposure Factors Handbook 2011 Edition (Final)

    EPA Science Inventory

    edition_cover.jpg" vspace = "5" hspace="5" align="right" border="2" alt="Cover of the Exposure Factors Handbook: 2011 Edition"> The mission of National Center for Environmental Assessment (NCEA) of EPA's ...

  6. Applications of genome editing in insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insect genome editing was first reported 1991 in Drosophila melanogaster but the technology used was not portable to other species. Not until the recent development of facile, engineered DNA endonuclease systems has gene editing become widely available to insect scientists. Most applications in inse...

  7. Novel modes of RNA editing in mitochondria

    PubMed Central

    Moreira, Sandrine; Valach, Matus; Aoulad-Aissa, Mohamed; Otto, Christian; Burger, Gertraud

    2016-01-01

    Gene structure and expression in diplonemid mitochondria are unparalleled. Genes are fragmented in pieces (modules) that are separately transcribed, followed by the joining of module transcripts to contiguous RNAs. Some instances of unique uridine insertion RNA editing at module boundaries were noted, but the extent and potential occurrence of other editing types remained unknown. Comparative analysis of deep transcriptome and genome data from Diplonema papillatum mitochondria reveals ∼220 post-transcriptional insertions of uridines, but no insertions of other nucleotides nor deletions. In addition, we detect in total 114 substitutions of cytosine by uridine and adenosine by inosine, amassed into unusually compact clusters. Inosines in transcripts were confirmed experimentally. This is the first report of adenosine-to-inosine editing of mRNAs and ribosomal RNAs in mitochondria. In mRNAs, editing causes mostly amino-acid additions and non-synonymous substitutions; in ribosomal RNAs, it permits formation of canonical secondary structures. Two extensively edited transcripts were compared across four diplonemids. The pattern of uridine-insertion editing is strictly conserved, whereas substitution editing has diverged dramatically, but still rendering diplonemid proteins more similar to other eukaryotic orthologs. We posit that RNA editing not only compensates but also sustains, or even accelerates, ultra-rapid evolution of genome structure and sequence in diplonemid mitochondria. PMID:27001515

  8. Accounting for Independent Schools. Second Edition.

    ERIC Educational Resources Information Center

    National Association of Independent Schools, Boston, MA.

    This is a thoroughly revised edition of the 1969 publication, "Accounting for Independent Schools," a guide that attempted to codify basic accounting principles and practices for specific application to independent schools. The focus of the second edition is more on refining practices than on initiating them, and more on extending the managerial…

  9. Non-GMO genetically edited crop plants.

    PubMed

    Kanchiswamy, Chidananda Nagamangala; Malnoy, Mickael; Velasco, Riccardo; Kim, Jin-Soo; Viola, Roberto

    2015-09-01

    Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome editing complex is degraded in the recipient cells. PMID:25978870

  10. America's Children and the Environment, Third Edition

    EPA Science Inventory

    America's Children and the Environment is the U.S. EPA's report of children's environmental health indicators. Two editions of the report have been published, in 2000 and 2003, and a website is maintained with updated values for the indicators. The new Third Edition of America'...

  11. AV Instruction Media and Methods; Third Edition.

    ERIC Educational Resources Information Center

    Brown, James W., Ed.; And Others

    In addition to updating material covered in the second edition and providing more illustrative material, this edition attempts to offer a systematic approach to the integration of instructional aids into the curriculum. It discusses the use of significant new developments in instructional technology-multimedia packages or kits, programed…

  12. Exploring the RNA editing potential of RNA-Seq data by ExpEdit.

    PubMed

    D'Antonio, Mattia; Picardi, Ernesto; Castrignanò, Tiziana; D'Erchia, Anna Maria; Pesole, Graziano

    2015-01-01

    Revealing the impact of A-to-I RNA editing in RNA-Seq experiments is relevant in humans because RNA editing can influence gene expression. In addition, its deregulation has been linked to a variety of human diseases. Exploiting the RNA editing potential in complete RNA-Seq datasets, however, is a challenging task. Indeed, no dedicated software is available, and sometimes deep computational skills and appropriate hardware resources are required. To explore the impact of known RNA editing events in massive transcriptome sequencing experiments, we developed the ExpEdit web service application. In the present work, we provide an overview of ExpEdit as well as methodologies to investigate known RNA editing in human RNA-Seq datasets. PMID:25577388

  13. Ebola virus RNA editing depends on the primary editing site sequence and an upstream secondary structure.

    PubMed

    Mehedi, Masfique; Hoenen, Thomas; Robertson, Shelly; Ricklefs, Stacy; Dolan, Michael A; Taylor, Travis; Falzarano, Darryl; Ebihara, Hideki; Porcella, Stephen F; Feldmann, Heinz

    2013-01-01

    Ebolavirus (EBOV), the causative agent of a severe hemorrhagic fever and a biosafety level 4 pathogen, increases its genome coding capacity by producing multiple transcripts encoding for structural and nonstructural glycoproteins from a single gene. This is achieved through RNA editing, during which non-template adenosine residues are incorporated into the EBOV mRNAs at an editing site encoding for 7 adenosine residues. However, the mechanism of EBOV RNA editing is currently not understood. In this study, we report for the first time that minigenomes containing the glycoprotein gene editing site can undergo RNA editing, thereby eliminating the requirement for a biosafety level 4 laboratory to study EBOV RNA editing. Using a newly developed dual-reporter minigenome, we have characterized the mechanism of EBOV RNA editing, and have identified cis-acting sequences that are required for editing, located between 9 nt upstream and 9 nt downstream of the editing site. Moreover, we show that a secondary structure in the upstream cis-acting sequence plays an important role in RNA editing. EBOV RNA editing is glycoprotein gene-specific, as a stretch encoding for 7 adenosine residues located in the viral polymerase gene did not serve as an editing site, most likely due to an absence of the necessary cis-acting sequences. Finally, the EBOV protein VP30 was identified as a trans-acting factor for RNA editing, constituting a novel function for this protein. Overall, our results provide novel insights into the RNA editing mechanism of EBOV, further understanding of which might result in novel intervention strategies against this viral pathogen. PMID:24146620

  14. Fuel Cell Handbook, Fifth Edition

    SciTech Connect

    Energy and Environmental Solutions

    2000-10-31

    Progress continues in fuel cell technology since the previous edition of the Fuel Cell Handbook was published in November 1998. Uppermost, polymer electrolyte fuel cells, molten carbonate fuel cells, and solid oxide fuel cells have been demonstrated at commercial size in power plants. The previously demonstrated phosphoric acid fuel cells have entered the marketplace with more than 220 power plants delivered. Highlighting this commercial entry, the phosphoric acid power plant fleet has demonstrated 95+% availability and several units have passed 40,000 hours of operation. One unit has operated over 49,000 hours. Early expectations of very low emissions and relatively high efficiencies have been met in power plants with each type of fuel cell. Fuel flexibility has been demonstrated using natural gas, propane, landfill gas, anaerobic digester gas, military logistic fuels, and coal gas, greatly expanding market opportunities. Transportation markets worldwide have shown remarkable interest in fuel cells; nearly every major vehicle manufacturer in the U.S., Europe, and the Far East is supporting development. This Handbook provides a foundation in fuel cells for persons wanting a better understanding of the technology, its benefits, and the systems issues that influence its application. Trends in technology are discussed, including next-generation concepts that promise ultrahigh efficiency and low cost, while providing exceptionally clean power plant systems. Section 1 summarizes fuel cell progress since the last edition and includes existing power plant nameplate data. Section 2 addresses the thermodynamics of fuel cells to provide an understanding of fuel cell operation at two levels (basic and advanced). Sections 3 through 8 describe the six major fuel cell types and their performance based on cell operating conditions. Alkaline and intermediate solid state fuel cells were added to this edition of the Handbook. New information indicates that manufacturers have stayed

  15. Image editing with spatiograms transfer.

    PubMed

    Papadakis, Nicolas; Bugeau, Aurélie; Caselles, Vicent

    2012-05-01

    Histogram equalization is a well-known method for image contrast enhancement. Nevertheless, as histograms do not include any information on the spatial repartition of colors, their application to local image editing problems remains limited. To cope with this lack of spatial information, spatiograms have been recently proposed for tracking purposes. A spatiogram is an image descriptor that combines a histogram with the mean and the variance of the position of each color. In this paper, we address the problem of local retouching of images by proposing a variational method for spatiogram transfer. More precisely, a reference spatiogram is used to modify the color value of a given region of interest of the processed image. Experiments on shadow removal and inpainting demonstrate the strength of the proposed approach. PMID:22249712

  16. Evolution of RNA editing in trypanosome mitochondria

    PubMed Central

    Simpson, Larry; Thiemann, Otavio H.; Savill, Nicholas J.; Alfonzo, Juan D.; Maslov, D. A.

    2000-01-01

    Two different RNA editing systems have been described in the kinetoplast-mitochondrion of trypanosomatid protists. The first involves the precise insertion and deletion of U residues mostly within the coding regions of maxicircle-encoded mRNAs to produce open reading frames. This editing is mediated by short overlapping complementary guide RNAs encoded in both the maxicircle and the minicircle molecules and involves a series of enzymatic cleavage-ligation steps. The second editing system is a C34 to U34 modification in the anticodon of the imported tRNATrp, thereby permitting the decoding of the UGA stop codon as tryptophan. U-insertion editing probably originated in an ancestor of the kinetoplastid lineage and appears to have evolved in some cases by the replacement of the original pan-edited cryptogene with a partially edited cDNA. The driving force for the evolutionary fixation of these retroposition events was postulated to be the stochastic loss of entire minicircle sequence classes and their encoded guide RNAs upon segregation of the single kinetoplast DNA network into daughter cells at cell division. A large plasticity in the relative abundance of minicircle sequence classes has been observed during cell culture in the laboratory. Computer simulations provide theoretical evidence for this plasticity if a random distribution and segregation model of minicircles is assumed. The possible evolutionary relationship of the C to U and U-insertion editing systems is discussed. PMID:10860961

  17. Changing genetic information through RNA editing

    NASA Technical Reports Server (NTRS)

    Maas, S.; Rich, A.

    2000-01-01

    RNA editing, the post-transcriptional alteration of a gene-encoded sequence, is a widespread phenomenon in eukaryotes. As a consequence of RNA editing, functionally distinct proteins can be produced from a single gene. The molecular mechanisms involved include single or multiple base insertions or deletions as well as base substitutions. In mammals, one type of substitutional RNA editing, characterized by site-specific base-modification, was shown to modulate important physiological processes. The underlying reaction mechanism of substitutional RNA editing involves hydrolytic deamination of cytosine or adenosine bases to uracil or inosine, respectively. Protein factors have been characterized that are able to induce RNA editing in vitro. A supergene family of RNA-dependent deaminases has emerged with the recent addition of adenosine deaminases specific for tRNA. Here we review the developments that have substantially increased our understanding of base-modification RNA editing over the past few years, with an emphasis on mechanistic differences, evolutionary aspects and the first insights into the regulation of editing activity.

  18. NRP 7th Edition: Are You Prepared?

    PubMed

    Zaichkin, Jeanette; Mccarney, Linda; Weiner, Gary

    2016-01-01

    The seventh edition of the American Academy of Pediatrics/American Heart Association Neonatal Resuscitation Program (NRP) materials must be in use by January 1, 2017. As in previous editions, changes in resuscitation science are based on an international review and consensus of current resuscitation science. The seventh edition NRP materials also include enhancements to training materials aimed at improving the quality of NRP instruction and providing the opportunity for ongoing education. A standardized approach to instructor training, an online Instructor Toolkit, eSim cases, and a new learning management system are among the new resources. PMID:27461196

  19. Marketing/Planning Library and Information Services. Second Edition.

    ERIC Educational Resources Information Center

    Weingand, Darlene E.

    In the first edition of this book, the concepts of marketing and planning library and information services were presented as effective managerial strategies. Several paragraphs from the introduction to the first edition are reproduced, with author commentary, in this edition as an affirmation that the message is still true. In this second edition,…

  20. RNA editing generates cellular subsets with diverse sequence within populations.

    PubMed

    Harjanto, Dewi; Papamarkou, Theodore; Oates, Chris J; Rayon-Estrada, Violeta; Papavasiliou, F Nina; Papavasiliou, Anastasia

    2016-01-01

    RNA editing is a mutational mechanism that specifically alters the nucleotide content in transcribed RNA. However, editing rates vary widely, and could result from equivalent editing amongst individual cells, or represent an average of variable editing within a population. Here we present a hierarchical Bayesian model that quantifies the variance of editing rates at specific sites using RNA-seq data from both single cells, and a cognate bulk sample to distinguish between these two possibilities. The model predicts high variance for specific edited sites in murine macrophages and dendritic cells, findings that we validated experimentally by using targeted amplification of specific editable transcripts from single cells. The model also predicts changes in variance in editing rates for specific sites in dendritic cells during the course of LPS stimulation. Our data demonstrate substantial variance in editing signatures amongst single cells, supporting the notion that RNA editing generates diversity within cellular populations. PMID:27418407

  1. RNA editing generates cellular subsets with diverse sequence within populations

    PubMed Central

    Harjanto, Dewi; Papamarkou, Theodore; Oates, Chris J.; Rayon-Estrada, Violeta; Papavasiliou, F. Nina; Papavasiliou, Anastasia

    2016-01-01

    RNA editing is a mutational mechanism that specifically alters the nucleotide content in transcribed RNA. However, editing rates vary widely, and could result from equivalent editing amongst individual cells, or represent an average of variable editing within a population. Here we present a hierarchical Bayesian model that quantifies the variance of editing rates at specific sites using RNA-seq data from both single cells, and a cognate bulk sample to distinguish between these two possibilities. The model predicts high variance for specific edited sites in murine macrophages and dendritic cells, findings that we validated experimentally by using targeted amplification of specific editable transcripts from single cells. The model also predicts changes in variance in editing rates for specific sites in dendritic cells during the course of LPS stimulation. Our data demonstrate substantial variance in editing signatures amongst single cells, supporting the notion that RNA editing generates diversity within cellular populations. PMID:27418407

  2. Research Review: Magazine Editors and Editing Practices.

    ERIC Educational Resources Information Center

    Jolliffe, Lee

    1994-01-01

    Reviews and critiques literature in the subfield of magazine editing research, chiefly biographical studies of individual editors and various types of studies of editorial practices, including surveys, magazine content analyses, and close qualitative examinations of editors' relationships with others. (SR)

  3. ASTM standards on thermocouples, 2nd edition

    SciTech Connect

    Not Available

    1986-01-01

    This edition has been expanded to include sixteen selected ASTM methods and practices on thermocouples. It also contains a Temperature Electromotive Force (EMF) Table for the NICROSIL-NISIL Thermocouple System.

  4. An Automatic Editing Algorithm for GPS data

    NASA Astrophysics Data System (ADS)

    Blewitt, Geoffrey

    1990-03-01

    An algorithm has been developed to edit automatically Global Positioning System data such that outlier deletion, cycle slip identification and correction are independent of clock instability, selective availability, receiver-satellite kinematics, and tropospheric conditions. This algorithm, called TurboEdit, operates on undifferenced, dual frequency carrier phase data, and requires (1) the use of P code pseudorange data and (2) a smoothly varying ionospheric electron content. The latter requirement can be relaxed if the analysis software incorporates ambiguity resolution techniques to estimate unresolved cycle slip parameters. TurboEdit was tested on the large data set from the CASA Uno experiment, which contained over 2500 cycle slips. Analyst intervention was required on 1% of the station-satellite passes, almost all of these problems being due to difficulties in extrapolating variations in the ionospheric delay. The algorithm is presently being adapted for real time data editing in the Rogue receiver for continuous monitoring applications.

  5. A genome editing primer for the hematologist.

    PubMed

    Hoban, Megan D; Bauer, Daniel E

    2016-05-26

    Gene editing enables the site-specific modification of the genome. These technologies have rapidly advanced such that they have entered common use in experimental hematology to investigate genetic function. In addition, genome editing is becoming increasingly plausible as a treatment modality to rectify genetic blood disorders and improve cellular therapies. Genome modification typically ensues from site-specific double-strand breaks and may result in a myriad of outcomes. Even single-strand nicks and targeted biochemical modifications that do not permanently alter the DNA sequence (epigenome editing) may be powerful instruments. In this review, we examine the various technologies, describe their advantages and shortcomings for engendering useful genetic alterations, and consider future prospects for genome editing to impact hematology. PMID:27053532

  6. An automatic editing algorithm for GPS data

    NASA Technical Reports Server (NTRS)

    Blewitt, Geoffrey

    1990-01-01

    An algorithm has been developed to edit automatically Global Positioning System data such that outlier deletion, cycle slip identification, and correction are independent of clock instability, selective availability, receiver-satellite kinematics, and tropospheric conditions. This algorithm, called TurboEdit, operates on undifferenced, dual frequency carrier phase data, and requires the use of P code pseudorange data and a smoothly varying ionospheric electron content. TurboEdit was tested on the large data set from the CASA Uno experiment, which contained over 2500 cycle slips.Analyst intervention was required on 1 percent of the station-satellite passes, almost all of these problems being due to difficulties in extrapolating variations in the ionospheric delay. The algorithm is presently being adapted for real time data editing in the Rogue receiver for continuous monitoring applications.

  7. The New Grolier Multimedia Encyclopedia 1994 Edition.

    ERIC Educational Resources Information Center

    Online Libraries and Microcomputers, 1994

    1994-01-01

    Reviews the 1994 edition of "The New Grolier Multimedia Encyclopedia" which is available on CD-ROM. Topics addressed include video files; content; ease of use; system requirements; cost; and print capabilities. (LRW)

  8. Computing services writing and editing standards

    SciTech Connect

    Caruthers, C.M.; Heiberger, A.A.

    1987-11-01

    Computing Services Writing and Editing Standards explains how to write and organize technical information clearly and concisely; it also establishes Argonne National Laboratory Computing Services standards for grammar, usage, style, formats, and publication procedures. This manual will enable writers to produce better documents consistent with Computing Services standards in less time. Applying the specified principles will also speed up the editing, review, and revision processes.

  9. Looking forward to genetically edited fruit crops.

    PubMed

    Nagamangala Kanchiswamy, Chidananda; Sargent, Daniel James; Velasco, Riccardo; Maffei, Massimo E; Malnoy, Mickael

    2015-02-01

    The availability of genome sequences for many fruit crops has redefined the boundaries of genetic engineering and genetically modified (GM) crop plants. However commercialization of GM crops is hindered by numerous regulatory and social hurdles. Here, we focus on recently developed genome-editing tools for fruit crop improvement and their importance from the consumer perspective. Challenges and opportunities for the deployment of new genome-editing tools for fruit plants are also discussed. PMID:25129425

  10. An efficient 1H/31P double-resonance solid-state NMR probe that utilizes a scroll coil

    PubMed Central

    Grant, Christopher V.; Sit, Siu-Ling; De Angelis, Anna A.; Khuong, Kelli S.; Wu, Chin H.; Plesniak, Leigh A.; Opella, Stanley J.

    2007-01-01

    The construction and performance of a scroll coil double-resonance probe for solid-state NMR on stationary samples is described. The advantages of the scroll coil at the high resonance frequencies of 1H and 31P include: high efficiency, minimal perturbations of tuning by a wide range of samples, minimal RF sample heating of high dielectric samples of biopolymers in aqueous solution, and excellent RF homogeneity. The incorporation of a cable tie cinch for mechanical stability of the scroll coil is described. Experimental results obtained on a Hunter Killer Peptide 1 (HKP1) interacting with phospholipid bilayers of varying lipid composition demonstrate the capabilities of this probe on lossy aqueous samples. PMID:17719813

  11. An efficient (1)H/(31)P double-resonance solid-state NMR probe that utilizes a scroll coil.

    PubMed

    Grant, Christopher V; Sit, Siu-Ling; De Angelis, Anna A; Khuong, Kelli S; Wu, Chin H; Plesniak, Leigh A; Opella, Stanley J

    2007-10-01

    The construction and performance of a scroll coil double-resonance probe for solid-state NMR on stationary samples is described. The advantages of the scroll coil at the high resonance frequencies of (1)H and (31)P include: high efficiency, minimal perturbations of tuning by a wide range of samples, minimal RF sample heating of high dielectric samples of biopolymers in aqueous solution, and excellent RF homogeneity. The incorporation of a cable tie cinch for mechanical stability of the scroll coil is described. Experimental results obtained on a Hunter Killer Peptide 1 (HKP1) interacting with phospholipid bilayers of varying lipid composition demonstrate the capabilities of this probe on lossy aqueous samples. PMID:17719813

  12. High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing.

    PubMed

    Simpson, Rachel M; Bruno, Andrew E; Bard, Jonathan E; Buck, Michael J; Read, Laurie K

    2016-05-01

    Uridine insertion/deletion RNA editing in kinetoplastids entails the addition and deletion of uridine residues throughout the length of mitochondrial transcripts to generate translatable mRNAs. This complex process requires the coordinated use of several multiprotein complexes as well as the sequential use of noncoding template RNAs called guide RNAs. The majority of steady-state mitochondrial mRNAs are partially edited and often contain regions of mis-editing, termed junctions, whose role is unclear. Here, we report a novel method for sequencing entire populations of pre-edited partially edited, and fully edited RNAs and analyzing editing characteristics across populations using a new bioinformatics tool, the Trypanosome RNA Editing Alignment Tool (TREAT). Using TREAT, we examined populations of two transcripts, RPS12 and ND7-5', in wild-typeTrypanosoma brucei We provide evidence that the majority of partially edited sequences contain junctions, that intrinsic pause sites arise during the progression of editing, and that the mechanisms that mediate pausing in the generation of canonical fully edited sequences are distinct from those that mediate the ends of junction regions. Furthermore, we identify alternatively edited sequences that constitute plausible alternative open reading frames and identify substantial variability in the 5' UTRs of both canonical and alternatively edited sequences. This work is the first to use high-throughput sequencing to examine full-length sequences of whole populations of partially edited transcripts. Our method is highly applicable to current questions in the RNA editing field, including defining mechanisms of action for editing factors and identifying potential alternatively edited sequences. PMID:26908922

  13. [Textual research on the edition of WU Kun's books].

    PubMed

    Wang, Xu-Guang; Lu, Xiang

    2013-03-01

    WU Kun is a famous physician of the Xin'an School. He wrote a 6-volume Yi fang kao (Textual Research on Recipes) in 1584, a 24-volume Wu zhu su wen (Wu's Annotation of Plain Questions) in 1594, a 6-volume Zhen fang liu ji (Six Collections of Acupuncture and Prescription) in 1618 block-printed and funded by CHENG Biao, and a 2-volume Mai yu (Language of Pulse) with writing date unknown. For Yi fang kao, there were 4 editions, including Ming-dynasty block-printed edition, Japanese and Korean block-printed edition, old hand-copied edition and modern printing edition. For Wu zhu su wen, there were 5 editions, Ming-Qing-dynasty block-printed edition, Japanese block-printed edition, old hand-copied edition, photocopy and modern edition. For Zhen fang liu ji, there were Ming-dynasty block-printed edition, hand-copied edition, photocopy and modern edition. Mai yu always was printed together with Yi fang kao, and its editions were same as those of Yi fang kao, except some modern printing edition with Mai yu excluded. PMID:24135480

  14. Applications of genome editing in insects.

    PubMed

    Reid, William; O'Brochta, David A

    2016-02-01

    Insect genome editing was first reported 1991 in Drosophila melanogaster but the technology used was not portable to other species. Not until the recent development of facile, engineered DNA endonuclease systems has gene editing become widely available to insect scientists. Most applications in insects to date have been technical in nature but this is rapidly changing. Functional genomics and genetics-based insect control efforts will be major beneficiaries of the application of contemporary gene editing technologies. Engineered endonucleases like Cas9 make it possible to create powerful and effective gene drive systems that could be used to reduce or even eradicate specific insect populations. 'Best practices' for using Cas9-based editing are beginning to emerge making it easier and more effective to design and use but gene editing technologies still require traditional means of delivery in order to introduce them into somatic and germ cells of insects-microinjection of developing embryos. This constrains the use of these technologies by insect scientists. Insects created using editing technologies challenge existing governmental regulatory structures designed to manage genetically modified organisms. PMID:27436552

  15. On the evaluation of segmentation editing tools

    PubMed Central

    Heckel, Frank; Moltz, Jan H.; Meine, Hans; Geisler, Benjamin; Kießling, Andreas; D’Anastasi, Melvin; dos Santos, Daniel Pinto; Theruvath, Ashok Joseph; Hahn, Horst K.

    2014-01-01

    Abstract. Efficient segmentation editing tools are important components in the segmentation process, as no automatic methods exist that always generate sufficient results. Evaluating segmentation editing algorithms is challenging, because their quality depends on the user’s subjective impression. So far, no established methods for an objective, comprehensive evaluation of such tools exist and, particularly, intermediate segmentation results are not taken into account. We discuss the evaluation of editing algorithms in the context of tumor segmentation in computed tomography. We propose a rating scheme to qualitatively measure the accuracy and efficiency of editing tools in user studies. In order to objectively summarize the overall quality, we propose two scores based on the subjective rating and the quantified segmentation quality over time. Finally, a simulation-based evaluation approach is discussed, which allows a more reproducible evaluation without the need for human input. This automated evaluation complements user studies, allowing a more convincing evaluation, particularly during development, where frequent user studies are not possible. The proposed methods have been used to evaluate two dedicated editing algorithms on 131 representative tumor segmentations. We show how the comparison of editing algorithms benefits from the proposed methods. Our results also show the correlation of the suggested quality score with the qualitative ratings. PMID:26158063

  16. Book Review: New Perspectives on Technical Editing

    NASA Astrophysics Data System (ADS)

    Murphy, A. J. (Ed.); Sterken, Christiaan

    2012-08-01

    New Perspectives on Technical Editing by Avon J. Murphy (ed.) ISBN : 978-0895033949 (2010) Baywood Publishing Company Inc, Hardcover, 210 pages, 35.5 GBP This book presents a collection of 10 chapters dealing with diverse aspects of technical editing (ie, editorial planning, and analysis and structural changes made to other people's technological documents): research in technical editing, trends and teaching of technical editing, copyediting, and technical journal editing. The role and function of the modern journal and book editor is also dealt with in detail. Each chapter is written by an expert in the field: senior editors, university professors in technical communication, technical writers and linguists. The ever-evolving role of the editor is clearly elucidated in several historical reviews, and in the descriptions of the expectations for the future. A very striking aspect of this book is its extensive collection of bibliographic resources: every chapter lists dozens of very useful references, and the closing chapter, and annotated bibliography, contain many not so well known references, and are most useful. All in all, the book is a treasure trove listing more than 400 references, in addition to numerous webpage URLs embedded in the texts. The book is designed to help the reader to understand current practices and norms in technical editing, and to help to take action in editing as well as in teaching and educating would-be editors. The audience for this book thus includes editors and teachers, but also writers, researchers and students. A deep reading of this book will result in a better understanding of the difference between full technical editing and its much narrower component so well known as copyediting, and will convince any prospective editor that editing should not be undertaken if the people involved do not master the art of precision and accuracy in technical (as well as in human) communication, do not possess the technical know how and computer

  17. Residential Wiring. Fourth Edition. Teacher Edition [and] Student Guide [and] Student Workbook.

    ERIC Educational Resources Information Center

    Taylor, Mark

    Residential Wiring, the second publication in a series of three wiring publications, prepares students for entry-level employment in the residential wiring trade. Instructional materials include a teacher edition, student guide, and student workbook. The teacher edition begins with introductory pages, including a training and competency profile,…

  18. Biological Science: An Ecological Approach. BSCS Green Version. Teacher's Edition. Sixth Edition.

    ERIC Educational Resources Information Center

    Biological Sciences Curriculum Study, Colorado Springs.

    This book is the teacher's edition to the 1987 edition of the Biological Sciences Curriculum Study Green Version textbook. It contains directions for teaching with this version, a description of the accompanying materials, teaching strategies by chapters, lists of useful software, safety guidelines, a materials list, chemical safety information,…

  19. The Express-Lane Edit: Making Editing Useful for Young Adolescents

    ERIC Educational Resources Information Center

    Anderson, Jeff

    2008-01-01

    Editing is a powerful tool for writers, but are our methods of teaching it really demonstrating that power for young adolescents? The author, frustrated with students' inability to edit, blames his own approach and, beginning with a grocery store epiphany, works to develop a more effective system. Elements of his successful approach include time…

  20. Re-editing the paradigm of Cytidine (C) to Uridine (U) RNA editing

    PubMed Central

    Fossat, Nicolas; Tam, Patrick P L

    2014-01-01

    Cytidine (C) to Uridine (U) RNA editing is a post-trancriptional modification that until recently was known to only affect Apolipoprotein b (Apob) RNA and minimally require 2 components of the C to U editosome, the deaminase APOBEC1 and the RNA-binding protein A1CF. Our latest work has identified a novel RNA-binding protein, RBM47, as a core component of the editosome, which can substitute A1CF for the editing of ApoB mRNA. In addition, new RNA species that are subjected to C to U editing have been identified. Here, we highlight these recent discoveries and discuss how they change our view of the composition of the C to U editing machinery and expand our knowledge of the functional attributes of C to U RNA editing. PMID:25585043

  1. Triplex-mediated genome targeting and editing.

    PubMed

    Reza, Faisal; Glazer, Peter M

    2014-01-01

    Genome targeting and editing in vitro and in vivo can be achieved through an interplay of exogenously introduced molecules and the induction of endogenous recombination machinery. The former includes a repertoire of sequence-specific binding molecules for targeted induction and appropriation of this machinery, such as by triplex-forming oligonucleotides (TFOs) or triplex-forming peptide nucleic acids (PNAs) and recombinagenic donor DNA, respectively. This versatile targeting and editing via recombination approach facilitates high-fidelity and low-off-target genome mutagenesis, repair, expression, and regulation. Herein, we describe the current state-of-the-art in triplex-mediated genome targeting and editing with a perspective towards potential translational and therapeutic applications. We detail several materials and methods for the design, delivery, and use of triplex-forming and recombinagenic molecules for mediating and introducing specific, heritable, and safe genomic modifications. Furthermore we denote some guidelines for endogenous genome targeting and editing site identification and techniques to test targeting and editing efficiency. PMID:24557900

  2. BOOK REVIEW: Quantum Gravity: third edition Quantum Gravity: third edition

    NASA Astrophysics Data System (ADS)

    Rovelli, Carlo

    2012-09-01

    The request by Classical and Quantum Gravity to review the third edition of Claus Kiefer's 'Quantum Gravity' puts me in a slightly awkward position. This is a remarkably good book, which every person working in quantum gravity should have on the shelf. But in my opinion quantum gravity has undergone some dramatic advances in the last few years, of which the book makes no mention. Perhaps the omission only attests to the current vitality of the field, where progress is happening fast, but it is strange for me to review a thoughtful, knowledgeable and comprehensive book on my own field of research, which ignores what I myself consider the most interesting results to date. Kiefer's book is unique as a broad introduction and a reliable overview of quantum gravity. There are numerous books in the field which (often notwithstanding titles) focus on a single approach. There are also countless conference proceedings and article collections aiming to be encyclopaedic, but offering disorganized patchworks. Kiefer's book is a careful and thoughtful presentation of all aspects of the immense problem of quantum gravity. Kiefer is very learned, and brings together three rare qualities: he is pedagogical, he is capable of simplifying matter to the bones and capturing the essential, and he offers a serious and balanced evaluation of views and ideas. In a fractured field based on a major problem that does not yet have a solution, these qualities are precious. I recommend Kiefer's book to my students entering the field: to work in quantum gravity one needs a vast amount of technical knowledge as well as a grasp of different ideas, and Kiefer's book offers this with remarkable clarity. This novel third edition simplifies and improves the presentation of several topics, but also adds very valuable new material on quantum gravity phenomenology, loop quantum cosmology, asymptotic safety, Horava-Lifshitz gravity, analogue gravity, the holographic principle, and more. This is a testament

  3. Composition of the editing complex of Trypanosoma brucei.

    PubMed

    Stuart, K; Panigrahi, A K; Schnaufer, A; Drozdz, M; Clayton, C; Salavati, R

    2002-01-29

    The RNA editing that produces most functional mRNAs in trypanosomes is catalysed by a multiprotein complex. This complex catalyses the endoribonucleolytic cleavage, uridylate addition and removal, and RNA ligation steps of the editing process. Enzymatic and in vitro editing analyses reveal that each catalytic step contributes to the specificity of the editing and, together with the interaction between gRNA and the mRNA, results in precisely edited mRNAs. Tandem mass spectrometric analysis was used to identify the genes for several components of biochemically purified editing complexes. Their identity and presence in the editing complex were confirmed using immunochemical analyses utilizing mAbs specific to the editing complex components. The genes for two RNA ligases were identified. Genetic studies show that some, but not all, of the components of the complex are essential for editing. The TbMP52 RNA ligase is essential for editing while the TbMP48 RNA ligase is not. Editing was found to be essential in bloodstream form trypanosomes. This is surprising because mutants devoid of genes encoding RNAs that become edited survive as bloodstream forms but encouraging since editing complex components may be targets for chemotherapy. PMID:11839184

  4. Ethical and regulatory aspects of genome editing.

    PubMed

    Kohn, Donald B; Porteus, Matthew H; Scharenberg, Andrew M

    2016-05-26

    Gene editing is a rapidly developing area of biotechnology in which the nucleotide sequence of the genome of living cells is precisely changed. The use of genome-editing technologies to modify various types of blood cells, including hematopoietic stem cells, has emerged as an important field of therapeutic development for hematopoietic disease. Although these technologies offer the potential for generation of transformative therapies for patients suffering from myriad disorders of hematopoiesis, their application for therapeutic modification of primary human cells is still in its infancy. Consequently, development of ethical and regulatory frameworks that ensure their safe and effective use is an increasingly important consideration. Here, we review a number of issues that have the potential to impact the clinical implementation of genome-editing technologies, and suggest paths forward for resolving them such that new therapies can be safely and rapidly translated to the clinic. PMID:27053531

  5. Genome editing in zebrafish: a practical overview.

    PubMed

    Sertori, Robert; Trengove, Monique; Basheer, Faiza; Ward, Alister C; Liongue, Clifford

    2016-07-01

    Zebrafish is a powerful model for the study of vertebrate development, being amenable to a wide range of genetic and other manipulations to probe the molecular basis of development and its perturbation in disease. Over recent years, genome editing approaches have become increasingly used as an efficient and sophisticated approach to precisely engineer the zebrafish genome, which has further enhanced the utility of this organism. This review provides a practical overview of genome editing and its application in zebrafish research, including alternate strategies for introducing and screening for specific genetic changes. PMID:26654901

  6. Therapeutic Genome Editing: Prospects and Challenges

    PubMed Central

    Cox, David Benjamin Turitz; Platt, Randall Jeffrey; Zhang, Feng

    2015-01-01

    Recent advances in the development of genome editing technologies based on programmable nucleases have significantly improved our ability to make precise changes in the genomes of eukaryotic cells. Genome editing is already broadening our ability to elucidate the contribution of genetics to disease by facilitating the creation of more accurate cellular and animal models of pathological processes. A particularly tantalizing application of programmable nucleases is the potential to directly correct genetic mutations in affected tissues and cells to treat diseases that are refractory to traditional therapies. Here we discuss current progress towards developing programmable nuclease-based therapies as well as future prospects and challenges. PMID:25654603

  7. Astronomy For Dummies, 2nd Edition

    NASA Astrophysics Data System (ADS)

    Maran, Stephen P.

    2005-04-01

    An accessible guide to the wonders of the night sky, now updated From asteroids to black holes, from quasars to white dwarfs, this new edition of Astronomy For Dummies takes backyard stargazers on a grand tour of the universe. Featuring star maps, charts, gorgeous full-color photographs, and easy-to-follow explanations, this fact-filled guide gives readers a leg up on the basic principles of astronomy and shows how to get the most out of binoculars, telescopes, planetarium visits, and other fun astronomical activities. This updated edition includes an updated color signature and covers the many discoveries made in recent years, as well as new astronomy Web sites.

  8. Transportation Energy Data Book, Edition 19

    SciTech Connect

    Davis, S.C.

    1999-09-01

    The Transportation Energy Data Book: Edition 19 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Transportation Technologies in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest editions of the Data Book are available to a larger audience via the Internet (http://www-cta.ornl.gov/data/tedb.htm).

  9. [On two editions of Yunyu Xuanji and their source].

    PubMed

    Deng, Yue-E

    2013-01-01

    Yunyu Xuanji is considered as one of the 400 key ancient books in the 'TCM Ancient Books Protection and Use Project' by the State Bureau of TCM. Two editions of this book are hand-written copies. The Qianyao edition was finished in the 52(nd) year of the Kangxi Period (1713). The other edition (Taoshi Xianyishukoucongshu) was probably done in the Late Qing Dynasty or the period of the Republic of China. By careful comparison, the contents of the two editions are basically the same, and only the preface of the former edition has been given some changes, which were probably made by later generations. The second manuscript was written in a more standard mode. Therefore the two editions were written according to the same literature and it is possible that the latter one is the copied edition of the Qianyao edition. PMID:23879984

  10. A systematic study of genetic algorithms with genotype editing

    SciTech Connect

    Huang, C. F.; Rocha, L. M.

    2004-01-01

    This paper presents our systematic study on an RNA-editing computational model of Genetic Algorithms (GA). This model is constructed based on several genetic editing characteristics that are gleaned from the RNA editing system as observed in several organisms. We have expanded the traditional Genetic Algorithm with artificial editing mechanisms as proposed by [15]. The incorporation of editing mechanisms provides a means for artificial agents with genetic descriptions to gain greater phenotypic plasticity, which may be environmentally regulated. The systematic study of this RNA-editing model has shed some light into the evolutionary implications of RNA editing and how to select proper RNA editors for design of more robust GAS. The results will also show promising applications to complex real-world problems. We expect that the framework proposed will both facilitate determining the evolutionary role of RNA editing in biology, and advance the current state of research in Evolutionary Computation.

  11. Shielded Metal Arc Welding and Carbon Arc Cutting--Air. Teacher Edition [and] Student Edition [and] Student Workbook. Third Edition.

    ERIC Educational Resources Information Center

    Harper, Eddie; Knapp, John

    This document contains the teacher and student texts and student workbook for a secondary-level course in shielded metal arc welding (SMAW) and carbon arc cutting that consists of units on the following topics: SMAW safety; SMAW equipment, applications, and techniques; hardfacing; and carbon arc cutting--air. The teacher edition includes the…

  12. Gender Balance Analysis of the Metropolitan Achievement Tests, 1978 Edition.

    ERIC Educational Resources Information Center

    Jensen, Marjane; Beck, Michael D.

    The 1978 edition of the Metropolitan Achievement Tests was analyzed for sex stereotyping and for the use of male, female, or neuter references and a comparison was made with the 1970 edition. There was less bias in the new edition, and there was relatively little sex stereotyping with respect to occupations, activities, and roles for females.…

  13. Annotated Bibliography on Offender Job Training and Placement. Second Edition.

    ERIC Educational Resources Information Center

    Clem, Constance, Ed.

    This second edition of an annotated bibliography on offender job training and placement lists nearly 400 items from the collection of the National Institute of Corrections (NIC) Information Center in Longmont, Colorado, including 85 items added since the first edition in 1997. Items new to this edition are marked with an asterisk. Citations have…

  14. Genetic Architecture of Mitochondrial Editing in Arabidopsis thaliana

    PubMed Central

    Bentolila, Stéphane; Elliott, Leah E.; Hanson, Maureen R.

    2008-01-01

    We have analyzed the mitochondrial editing behavior of two Arabidopsis thaliana accessions, Landsberg erecta (Ler) and Columbia (Col). A survey of 362 C-to-U editing sites in 33 mitochondrial genes was conducted on RNA extracted from rosette leaves. We detected 67 new editing events in A. thaliana rosette leaves that had not been observed in a prior study of mitochondrial editing in suspension cultures. Furthermore, 37 of the 441 C-to-U editing events reported in A. thaliana suspension cultures were not observed in rosette leaves. Forty editing sites that are polymorphic in extent of editing were detected between Col and Ler. Silent editing sites, which do not change the encoded amino acid, were found in a large excess compared to nonsilent sites among the editing events that differed between accessions and between tissue types. Dominance relationships were assessed for 15 of the most polymorphic sites by evaluating the editing values of the reciprocal hybrids. Dominance is more common in nonsilent sites than in silent sites, while additivity was observed only in silent sites. A maternal effect was detected for 8 sites. QTL mapping with recombinant inbred lines detected 12 major QTL for 11 of the 13 editing traits analyzed, demonstrating that efficiency of editing of individual mitochondrial C targets is generally governed by a major factor. PMID:17565941

  15. Soil Carbon Sequestration and the Greenhouse Effect (2nd Edition)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This volume is a second edition of the book “Soil Carbon Sequestration and The Greenhouse Effect”. The first edition was published in 2001 as SSSA Special Publ. #57. The present edition is an update of the concepts, processes, properties, practices and the supporting data. All chapters are new co...

  16. Administration of the Small Public Library. Fourth Edition.

    ERIC Educational Resources Information Center

    Weingand, Darlene E.

    Since the publication of its first edition in 1965, this book has been a standard resource for setting up and managing cutting-edge small public library facilities. Completely revised and updated, this fourth edition continues that tradition with many more figures (28 in this edition), case studies and sample policies, and new content on grant…

  17. The role of RNA editing in dynamic environments

    SciTech Connect

    Rocha, L. M.; Huang, C. F.

    2004-01-01

    This paper presents a computational methodology based on Genetic Algorithms with Genotype Editing (GAE) for investigating the role of RNA editing in dynamic environments. This model is constructed based on several genetic editing characteristics that are gleaned from the RNA editing system as observed in several organisms. We have previously expanded the traditional Genetic Algorithm (GA) with artificial editing mechanisms (Rocha, 1995, 1997), and studied the benefits of including straightforward Genotype Editing in GA for several machine learning problems (Huang and Rocha, 2003, 2004). We show that the incorporation of genotype editing provides a means for artificial agents with genetic descriptions to gain greater phenotypic plasticity. Artificial agents use genotype edition to their advantage by linking it to environmental context. The ability to link changes in the environment with editing parameters gives organisms an adaptive advantage as genotype expression can become contextually regulated. The study of this RNA editing model in changing environments has shed some light into the evolutionary implications of RNA editing. We expect that our methodology will both facilitate determining the evolutionary role of RNA editing in biology, and advance the current state of research in Evolutionary Computation and Artificial Life.

  18. Adenosine to Inosine editing frequency controlled by splicing efficiency.

    PubMed

    Licht, Konstantin; Kapoor, Utkarsh; Mayrhofer, Elisa; Jantsch, Michael F

    2016-07-27

    Alternative splicing and adenosine to inosine (A to I) RNA-editing are major factors leading to co- and post-transcriptional modification of genetic information. Both, A to I editing and splicing occur in the nucleus. As editing sites are frequently defined by exon-intron basepairing, mRNA splicing efficiency should affect editing levels. Moreover, splicing rates affect nuclear retention and will therefore also influence the exposure of pre-mRNAs to the editing-competent nuclear environment. Here, we systematically test the influence of splice rates on RNA-editing using reporter genes but also endogenous substrates. We demonstrate for the first time that the extent of editing is controlled by splicing kinetics when editing is guided by intronic elements. In contrast, editing sites that are exclusively defined by exonic structures are almost unaffected by the splicing efficiency of nearby introns. In addition, we show that editing levels in pre- and mature mRNAs do not match. This phenomenon can in part be explained by the editing state of an RNA influencing its splicing rate but also by the binding of the editing enzyme ADAR that interferes with splicing. PMID:27112566

  19. Human Resources Administration: A School-Based Perspective. Fourth Edition

    ERIC Educational Resources Information Center

    Smith, Richard

    2009-01-01

    Enhanced and updated, this Fourth Edition of Richard E. Smith's highly successful text examines the growing role of the principal in planning, hiring, staff development, supervision, and other human resource functions. The Fourth Edition includes new sections on ethics, induction, and the role of the mentor teacher. This edition also introduces…

  20. Genome-wide identification of RNA editing in hepatocellular carcinoma.

    PubMed

    Kang, Lin; Liu, Xiaoqiao; Gong, Zhoulin; Zheng, Hancheng; Wang, Jun; Li, Yingrui; Yang, Huanming; Hardwick, James; Dai, Hongyue; Poon, Ronnie T P; Lee, Nikki P; Mao, Mao; Peng, Zhiyu; Chen, Ronghua

    2015-02-01

    We did whole-transcriptome sequencing and whole-genome sequencing on nine pairs of Hepatocellular carcinoma (HCC) tumors and matched adjacent tissues to identify RNA editing events. We identified mean 26,982 editing sites with mean 89.5% canonical A→G edits in each sample using an improved bioinformatics pipeline. The editing rate was significantly higher in tumors than adjacent normal tissues. Comparing the difference between tumor and normal tissues of each patient, we found 7 non-synonymous tissue specific editing events including 4 tumor-specific edits and 3 normal-specific edits in the coding region, as well as 292 edits varying in editing degree. The significant expression changes of 150 genes associated with RNA editing were found in tumors, with 3 of the 4 most significant genes being cancer related. Our results show that editing might be related to higher gene expression. These findings indicate that RNA editing modification may play an important role in the development of HCC. PMID:25462863

  1. Standards of Good Practice for Education Abroad. Fourth Edition

    ERIC Educational Resources Information Center

    Forum on Education Abroad, 2011

    2011-01-01

    This fourth edition of the Forum on Education Abroad's "Standards of Good Practice for Education Abroad" augments previous editions of the "Standards." Since the last edition was published in 2008, Forum member institutions and organizations have implemented the Standards in program development and assessment, using the Standards in the Forum's…

  2. The Chloroplast Genome of Pellia endiviifolia: Gene Content, RNA-Editing Pattern, and the Origin of Chloroplast Editing

    PubMed Central

    Grosche, Christopher; Funk, Helena T.; Maier, Uwe G.; Zauner, Stefan

    2012-01-01

    RNA editing is a post-transcriptional process that can act upon transcripts from mitochondrial, nuclear, and chloroplast genomes. In chloroplasts, single-nucleotide conversions in mRNAs via RNA editing occur at different frequencies across the plant kingdom. These range from several hundred edited sites in some mosses and ferns to lower frequencies in seed plants and the complete lack of RNA editing in the liverwort Marchantia polymorpha. Here, we report the sequence and edited sites of the chloroplast genome from the liverwort Pellia endiviifolia. The type and frequency of chloroplast RNA editing display a pattern highly similar to that in seed plants. Analyses of the C to U conversions and the genomic context in which the editing sites are embedded provide evidence in favor of the hypothesis that chloroplast RNA editing evolved to compensate mutations in the first land plants. PMID:23221608

  3. The boundaries of partially edited transcripts are not conserved in kinetoplastids: implications for the guide RNA model of editing.

    PubMed Central

    Landweber, L F; Fiks, A G; Gilbert, W

    1993-01-01

    We have studied partially edited molecules for the cytochrome-c oxidase subunit III (COIII) transcript from two species of the insect trypanosome Herpetomonas. We found unexpected patterns of editing, in which editing does not proceed strictly 3' to 5', in 24 of 61 partially edited clones. A comparison of the partially edited molecules between the two kinetoplastid species revealed an 8- to 10-nt shift in precisely defined editing boundaries, sites at which editing pauses before binding of the next guide RNA after formation of a stable duplex between a guide RNA and mRNA. This suggests that the region of base pairing between individual guide RNAs and the COIII transcript is not strictly conserved in kinetoplastids, implying gradual evolution of the editing process. Images Fig. 2 Fig. 3 PMID:8415685

  4. An Active Approach to Hindi (Temporary Edition).

    ERIC Educational Resources Information Center

    Sharma, D.N.; Stone, James W.

    This preliminary edition of the Foreign Service Institute's text in elementary Hindi is intended to "make it possible for students to begin using Hindi, from the first hour of class, to obtain information and exchange opinions about topics which are important to them." It is based on Stevick's "Microwave" style of lesson organization, which…

  5. The Academic Job Search Handbook. Third Edition.

    ERIC Educational Resources Information Center

    Heiberger, Mary Morris; Vick, Julia Miller

    This handbook provides specific advice on all aspects of job-seeking in an increasingly tight academic market, from the timetable for the application process through seeking tenure. New material in this edition provides more information for candidates in scientific and technical fields and more advice for those applying to technical and community…

  6. Populations. Basic Edition. Science for Micronesia.

    ERIC Educational Resources Information Center

    Trust Territory of the Pacific Islands Dept. of Education, Saipan.

    This teacher's guide is for an elementary school science unit designed for use with third grade (or older) children in the Trust Territory of Micronesia. Although there is a degree of similarity to curriculum materials developed for the Science Curriculum Improvement Study, this Micronesian unit does not purport to be an adaptation or edition of…

  7. Regression Analysis by Example. 5th Edition

    ERIC Educational Resources Information Center

    Chatterjee, Samprit; Hadi, Ali S.

    2012-01-01

    Regression analysis is a conceptually simple method for investigating relationships among variables. Carrying out a successful application of regression analysis, however, requires a balance of theoretical results, empirical rules, and subjective judgment. "Regression Analysis by Example, Fifth Edition" has been expanded and thoroughly…

  8. Part C Updates. 10th Edition

    ERIC Educational Resources Information Center

    Goode, Sue; Lazara, Alex; Danaher, Joan

    2008-01-01

    "Part C Updates" is a compilation of information on various aspects of the Early Intervention Program for Infants and Toddlers with Disabilities (Part C) of the Individuals with Disabilities Education Act (IDEA). This is the tenth volume in a series of compilations, which included two editions of Part H Updates, the former name of the program.…

  9. Enriching Student Teaching Relationships. Supervising Teacher Edition.

    ERIC Educational Resources Information Center

    Clothier, Grant; Kingsley, Elizabeth

    This training series was developed to improve the working relationships between supervising teachers and their student teachers. This supervising teacher's edition contains suggestions for such teachers as regards various activities dealing with the supervising/teaching situation, behavior problems, change, conference sessions, communication,…

  10. Introduction to Energy - 2nd Edition

    NASA Astrophysics Data System (ADS)

    Cassedy, Edward S.; Grossman, Peter Z.

    1998-12-01

    Energy issues such as pollution, resource depletion, global warming, nuclear power and waste are problems that demand timely solutions. This book provides a critical examination of the resources, market forces, and social impacts of modern energy production. The book addresses the dilemmas that have arisen due to society's crucial dependence on energy, particularly fossil fuels, and explores the available alternative energy producing technologies. The second edition has increased emphasis on those issues at the forefront of the current energy debate: energy sustainability, climate change, and the radical restructuring of the power industry due to de-regulation. Assuming no prior technical expertise and avoiding complex mathematical formulation, it is directed at a broad readership. The second edition will follow the first in proving especially useful as a textbook for undergraduate programs in Science, Technology and Society (STS), and as a supplementary text in a variety of courses which touch upon energy studies, including environmental and technology policy, environmental, mineral and business law, energy and resource economics. Fully updated second edition of successful first edition that was adopted on Science, Technology and Society courses Provides a critical examination of all aspects of modern energy production for non-technical readers For a broad readership from a variety of backgrounds

  11. The Complete Learning Disabilities Directory, 2007 Edition

    ERIC Educational Resources Information Center

    Grey House Publishing, 2006

    2006-01-01

    This is the thirteenth edition of the most comprehensive reference book covering the wide range of learning disabilities resources available nationwide. This directory contains well over 7,000 entries and covers the schools, learning centers, vocational training programs, associations and organizations, and government agencies involved in learning…

  12. Collecting and Interpreting Qualitative Materials. Third Edition

    ERIC Educational Resources Information Center

    Denzin, Norman K., Ed.; Lincoln, Yvonna, Ed.

    2007-01-01

    This book is the third volume of the paperback versions of "The SAGE Handbook of Qualitative Research, Third Edition." This portion of the handbook considers the tasks of collecting, analyzing, and interpreting empirical materials, and comprises the Handbook's Parts IV ("Methods of Collecting and Analyzing Empirical Materials") and V ("The Art and…

  13. Handbook of Qualitative Research. Second Edition.

    ERIC Educational Resources Information Center

    Denzin, Norman K., Ed.; Lincoln, Yvonna S., Ed.

    This handbook's second edition represents the state of the art for the theory and practice of qualitative inquiry. It features eight new topics, including autoethnography, critical race theory, applied ethnography, queer theory, and "testimonio"every chapter in the handbook has been thoroughly revised and updated. The book contains:"Preface" (1…

  14. Marching to Different Drummers. 2nd Edition.

    ERIC Educational Resources Information Center

    Guild, Pat Burke; Garger, Stephen

    First published in 1985, this revised edition focuses on diversity in education, exploring differences in style to help educators better fulfill their responsibilities and assist people in realizing their potential. Among the new chapters are a discussion of the importance of knowledge about students' culture, learning styles in light of recent…

  15. Guide to Productivity Improvement Projects. Third Edition.

    ERIC Educational Resources Information Center

    International City Management Association, Washington, DC.

    This edition features over 400 projects that have effected cost savings and/or improved services in a broad range of functional areas of city management, a jurisdictional index as well as a topical index, in-depth reports on selected projects, and project description forms that readers can use to share their successful projects with other readers.…

  16. Grants for Higher Education. 2012 Digital Edition

    ERIC Educational Resources Information Center

    Foundation Center, 2011

    2011-01-01

    This publication is only available as a downloadable file. See who's giving and getting grants in your field. Strengthen your search for funds with the Foundation Center's digital edition of "Grants for Higher Education." This new "Grant Guide" reveals the scope of current foundation giving in the field. You'll find descriptions of 19,705 grants…

  17. Grants for Children & Youth--2012 Digital Edition

    ERIC Educational Resources Information Center

    Foundation Center, 2011

    2011-01-01

    This publication is only available as a downloadable file. See who's giving and getting grants in your field. Strengthen your search for funds with the Foundation Center's digital edition of "Grants for Children & Youth." This new "Grant Guide" reveals the scope of current foundation giving in the field. You'll find descriptions of 37,992 grants…

  18. Grants for Employment--2012 Digital Edition

    ERIC Educational Resources Information Center

    Foundation Center, 2011

    2011-01-01

    This publication is only available as a downloadable file. See who's giving and getting grants in your field. Strengthen your search for funds with the Foundation Center's digital edition of "Grants for Employment." This new "Grant Guide" reveals the scope of current foundation giving in the field. You'll find descriptions of 4,129 grants of…

  19. Researching Society and Culture. Third Edition

    ERIC Educational Resources Information Center

    Seale, Clive, Ed.

    2012-01-01

    Clear, coherent and trusted, this book is the perfect guide to the main social research methods in use today. The much anticipated Third Edition of Clive Seale's bestselling title further expands its coverage to provide an authoritative introduction to all of the social research methods used to analyze qualitative and quantitative data. Written by…

  20. Compact Geography of the Netherlands. (Third Edition).

    ERIC Educational Resources Information Center

    Information and Documentation Centre for the Geography of the Netherlands, Utrecht.

    This document presents information on the physical and human geography of the Netherlands. In this third revised edition, the contents have been brought up-to-date and expanded. In addition, sections on physical planning and environmental problems have been added. The booklet is intended for use by geography classroom teachers and students at…

  1. More Than A Native Speaker, Revised Edition

    ERIC Educational Resources Information Center

    Snow, Don, Ed.

    2006-01-01

    This revised edition includes an expanded discussion of student-directed language learning, workbook activities for volunteer teachers enrolled in courses or studying the book individually, and a full array of culture-based discussion topics for use as supplementary activities or core material for an English course. The book includes 16 chapters.…

  2. Positive Behavior Support Training Curriculum. Second Edition

    ERIC Educational Resources Information Center

    Reid, Dennis H.; Parsons, Marsha B.

    2007-01-01

    The American Association on Intellectual and Developmental Disabilities' (AAIDD's) Positive Behavior Support Training Curriculum," Second Edition" ("PBSTC"), is a curriculum for training direct support personnel and their supervisors in the values and practices of Positive Behavior Support. The curriculum is designed for direct support persons and…

  3. The WRITEACOURSE Language: Programming Manual. Revised Edition.

    ERIC Educational Resources Information Center

    Zosel, Mary; And Others

    WRITEACOURSE is a programing language for man-computer interactions. It was originally designed for writing computer assisted instruction courses, but it can also be used to control a remote terminal in a variety of applications which involve display and editing of characters. It is not suited for applications which use the computer as an…

  4. 10 Tempting Image-Editing Tasks

    ERIC Educational Resources Information Center

    Guhin, Paula

    2009-01-01

    Asking students to manipulate digital photos on the computer is one of the easiest ways the author knows to engage their attention. It's fabulous fun for them and a great teaching tool for educators. In this article, the author presents 10 ways to impress students with image-editing software. These are: (1) filters are fascinating; (2) get a move…

  5. Designing Effective Instruction. 5th Edition

    ERIC Educational Resources Information Center

    Morrison, Gary R.; Ross, Steven M.; Kemp, Jerrold E.

    2006-01-01

    This valuable resource provides instructional designers with the guidance they need to meet the challenge of creating effective and efficient instruction. Maintaining a careful balance between theory and application, the Fifth Edition presents a practical, easy-to-follow approach to instructional design that can be applied to K-12 classrooms,…

  6. Media Research Techniques. 2nd Edition.

    ERIC Educational Resources Information Center

    Berger, Arthur Asa

    Suggesting it is a good idea for students to try their hands at doing media research themselves, this book presents a number of research projects that students will find interesting and that they can do with minimum experience in a limited amount of time. The second edition has added chapters on experimentation, historical research, comparative…

  7. The Art of Electronics - 2nd Edition

    NASA Astrophysics Data System (ADS)

    Horowitz, Paul; Hill, Winfield

    1989-09-01

    This is the thoroughly revised and updated second edition of the hugely successful The Art of Electronics. Widely accepted as the single authoritative text and reference on electronic circuit design, both analog and digital, the original edition sold over 125,000 copies worldwide and was translated into eight languages. The book revolutionized the teaching of electronics by emphasizing the methods actually used by citcuit designers - a combination of some basic laws, rules to thumb, and a large nonmathematical treatment that encourages circuit values and performance. The new Art of Electronics retains the feeling of informality and easy access that helped make the first edition so successful and popular. It is an ideal first textbook on electronics for scientists and engineers and an indispensable reference for anyone, professional or amateur, who works with electronic circuits. The best self-teaching book and reference book in electronics Simply indispensable, packed with essential information for all scientists and engineers who build electronic circuits Totally rewritten chapters on microcomputers and microprocessors The first edition of this book has sold over 100,000 copies in seven years, it has a market in virtually all research centres where electronics is important

  8. Does Money Matter in Education? Second Edition

    ERIC Educational Resources Information Center

    Baker, Bruce D.

    2016-01-01

    This second edition policy brief revisits the long and storied literature on whether money matters in providing a quality education. It includes research released since the original brief in 2012 and covers a handful of additional topics. Increasingly, political rhetoric adheres to the unfounded certainty that money does not make a difference in…

  9. PIRLS 2016 Assessment Framework. 2nd Edition

    ERIC Educational Resources Information Center

    Mullis, Ina V. S., Ed.; Martin, Michael O., Ed.

    2015-01-01

    The "PIRLS 2016 Assessment Framework, 2nd Edition" provides the foundation for the three international assessments planned as part of the International Association for the Evaluation of Educational Achievement's Progress in International Reading Literacy Study (PIRLS) 2016: PIRLS, PIRLS Literacy, and ePIRLS. PIRLS represents the…

  10. Television and the American Family. Second Edition.

    ERIC Educational Resources Information Center

    Bryant, Jennings, Ed.; Bryant, J. Alison, Ed.

    Noting drastic changes in both television and the family since the 1990 edition, this revised volume provides an extensive consideration of television's role in the American family, from the uses families make of television and how extensions such as remote controls and VCRs affect usage, to the meanings families have for television, families'…

  11. An ADHD Primer. 2nd Edition

    ERIC Educational Resources Information Center

    Weyandt, Lisa L.

    2007-01-01

    Filled with current, practical, and useful information for professionals and individuals, this second edition summarizes the literature concerning ADHD across the lifespan. It offers a better understanding of the disorder by addressing the potential causes of ADHD, the developmental course, and numerous treatment approaches. The author delivers…

  12. A Snapshot of Photo Editing Options

    ERIC Educational Resources Information Center

    Bolkan, J.V.

    2004-01-01

    Plenty of digital imaging professionals claim that Adobe's Photoshop CS is the best photo editing application money can buy. This document reviews Adobe's Photoshop CS and its worthy competitors. In addition to Adobe, the following programs are reviewed in this document: (1) Adobe Photoshop Elements 2.0; (2) Arcsoft PhotoImpression; (3) Jasc Paint…

  13. Ladybugs of South Dakota, 2nd edition

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Images of the 80 species of Coccinellidae, commonly known as lady beetles, that occur in South Dakota are presented in taxonomic order. The second edition updates information, including the addition of a species new to South Dakota. Information on each species includes genus-species name, sub-fami...

  14. Total Quality Management in Education. Second Edition.

    ERIC Educational Resources Information Center

    Sallis, Edward

    Quality is at the top of most agendas, and improving quality is probably the most important task facing any institution. In addition, quality is difficult to define or measure. This book, the second edition of "Total Quality Management in Education," introduces the key concepts of Total Quality Management (TQM) and demonstrates how they can be put…

  15. Children, Play, and Development. Fourth Edition

    ERIC Educational Resources Information Center

    Hughes, Fergus P.

    2010-01-01

    Children, Play, and Development, Fourth Edition, discusses the relationship of play to the physical, social, intellectual, and emotional growth of the child. Author Fergus P. Hughes focuses on the historical, sociocultural, and ethological context of play; the role of development in play; and the wide range of theories that provide a framework for…

  16. Brief Articles for Latino Parents, 1999 Edition.

    ERIC Educational Resources Information Center

    ERIC Clearinghouse on Rural Education and Small Schools, Charleston, WV.

    This packet contains six briefs developed specifically for Spanish-speaking Latino parents, and English translations of the briefs. These briefs state what researchers and practitioners have learned about various ways parents can help their children do well in school. Earlier editions of brief articles for parents have been used in various ways by…

  17. Engineered Viruses as Genome Editing Devices

    PubMed Central

    Chen, Xiaoyu; Gonçalves, Manuel A F V

    2016-01-01

    Genome editing based on sequence-specific designer nucleases, also known as programmable nucleases, seeks to modify in a targeted and precise manner the genetic information content of living cells. Delivering into cells designer nucleases alone or together with donor DNA templates, which serve as surrogate homologous recombination (HR) substrates, can result in gene knockouts or gene knock-ins, respectively. As engineered replication-defective viruses, viral vectors are having an increasingly important role as delivery vehicles for donor DNA templates and designer nucleases, namely, zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated Cas9 (CRISPR−Cas9) nucleases, also known as RNA-guided nucleases (RGNs). We review this dual role played by engineered viral particles on genome editing while focusing on their main scaffolds, consisting of lentiviruses, adeno-associated viruses, and adenoviruses. In addition, the coverage of the growing body of research on the repurposing of viral vectors as delivery systems for genome editing tools is complemented with information regarding their main characteristics, pros, and cons. Finally, this information is framed by a concise description of the chief principles, tools, and applications of the genome editing field as a whole. PMID:26336974

  18. A Dictionary of American Idioms. Third Edition.

    ERIC Educational Resources Information Center

    Makkai, Adam; And Others

    This revised, updated, and expanded edition defines more than 8000 idiomatic words and phrases of American English. Each alphabetical entry has a grammatical explanation and an example sentence. The dictionary, which includes a preface in nine different languages, is a tool to help increase fluency in English for students, business travelers, and…

  19. Needed Research in Business Education. Sixth Edition.

    ERIC Educational Resources Information Center

    Rader, Martha H.; Wilhelm, William J.

    Areas of business education that need to be researched were identified through a two-round study. First, 2 groups of more than 40 Delta Pi Epsilon members generated ideas for research topics at regional and national conferences. The resulting list of topics was edited and rated by a Delphi panel consisting of 15 educators from postsecondary and…

  20. Medication Administration Technician. Update. Teacher Edition.

    ERIC Educational Resources Information Center

    Oklahoma State Dept. of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This teacher's edition contains curriculum materials for an upgrading course for medication administration technicians who have completed initial training. The course consists of six units of instruction that cover the following: (1) controlling infection; (2) administering medication; (3) obtaining vital signs; (4) discussing legal issues and…

  1. Greenhouse Management and Operations. Teacher Edition.

    ERIC Educational Resources Information Center

    Gowdy, Mary Ann Schwartz

    This document is the teacher's edition of a module containing 16 instructional units covering competencies for students with career aspirations in horticulture. It is designed to provide high school students with an in-depth perspective of both the technical and the commercial aspects of running a greenhouse. The 16 units cover the following…

  2. The Complete Learning Disabilities Directory. 2011 Edition

    ERIC Educational Resources Information Center

    Grey House Publishing, 2010

    2010-01-01

    Published for over a decade, this directory continues to be a successful, sought-after resource, providing valuable information to professionals, families, and individuals in the learning disabilities community. Supported by the National Center for Learning Disabilities, this 2011 edition brings together the most up-to-date information on LD…

  3. Case Studies for Inclusive Schools. Second Edition

    ERIC Educational Resources Information Center

    Anderson, Peggy L.

    2005-01-01

    Case Studies for "Inclusive Schools, Second Edition" presents a sampling of case studies that contain realistic problems concerning inclusion issues for teacher education students to solve. This format was chosen because the case study approach to learning is gaining in popularity as it provides students with an opportunity to apply information…

  4. Writing in the Content Areas. Second Edition

    ERIC Educational Resources Information Center

    Benjamin, Amy

    2005-01-01

    Do you spend entirely too much time correcting your students' papers? Do your students' essays and term papers take side trips to nowhere? Is their writing riddled with mechanical errors? Do their lab reports and essays lack specificity and clarity? Writing in the Content Areas, Second Edition is for middle and high school content area teachers…

  5. ETC 408/508: Technical Editing

    ERIC Educational Resources Information Center

    Charlton, Michael

    2013-01-01

    The course will focus on the role of the editor in organizational settings, including creating successful writer/editor collaboration. Students will gain practice in editing documents for grammar, syntax, organization, style, emphasis, document design, graphics, and user-centered design. The course will provide an introduction to technology for…

  6. ETC 408/508: Technical Editing

    ERIC Educational Resources Information Center

    Charlton, Michael

    2013-01-01

    ETC 408/508: Technical Editing is a cross-listed undergraduate and graduate course at Missouri Western State University, an open admissions public university with approximately 6,000 students. 508 is an elective course for students in the Master of Applied Arts in Written Communication degree and highly recommended for those in the Technical…

  7. Teachers and the Law. Sixth Edition.

    ERIC Educational Resources Information Center

    Fischer, Louis; Schimmel, David; Stellman, Leslie

    This book is about teachers and the laws that affect them. New to this sixth edition are new court cases and a chapter that highlights likely controversies in the coming years, including school choice, high-stakes testing, control of the Internet, and gang clothing. The book is divided into two parts. Part I, "The Legal Aspects of Teaching,"…

  8. The NMC Horizon Report: 2013 Museum Edition

    ERIC Educational Resources Information Center

    Johnson, L.; Adams Becker, S.; Freeman, A.

    2013-01-01

    The "NMC Horizon Report: 2013 Museum Edition," is a co-production with the Marcus Institute for Digital Education in the Arts (MIDEA), and examines six emerging technologies for their potential impact on and use in education and interpretation within the museum environment: BYOD (Bring Your Own Device), crowdsourcing, electronic…

  9. School Safety. Communique Special Edition, Fall 1996.

    ERIC Educational Resources Information Center

    National Association of School Psychologists, Washington, DC.

    "Communique" is the "official newsletter of the National Association of School Psychologists" (NASP). This "Special Edition" of "Communique" is a compilation of twelve previously-published articles/pieces all of which touch on the theme of "School Safety." Articles in this issue include (1) "Creating Systemic Change: A Psychologist and Principal…

  10. Asia: A Guide to Paperbacks. Revised Edition.

    ERIC Educational Resources Information Center

    Embree, Ainslie T., Ed.

    The guide includes in-print titles which were listed in the original guide and the supplement, new titles which appeared between December 1965 and December 1967, books published in 1968 provided by some publishers, and a few titles omitted from earlier editions. All the books are listed alphabetically by author within five subject areas:…

  11. ASIA A Guide to Paperbacks. Revised Edition.

    ERIC Educational Resources Information Center

    Embree, Ainslie T., Ed.

    The guide includes in-print titles which were listed in the original guide and the supplement, new titles which appeared between December 1965 and December 1967, books published in 1968 provided by some publishers, and a few titles omitted from earlier editions. All the books are listed alphabetically by author within five subject areas:…

  12. Literature for Today's Young Adults. Second Edition.

    ERIC Educational Resources Information Center

    Nilsen, Alleen Pace; Donelson, Kenneth L.

    Reflecting the rapid changes that have occurred in young adult literature, this second edition provides teachers with the history and background needed to stay current with what adolescents are reading and how such literature can be taught. The book is organized much as a literature course is taught: first, an introduction to young adults and…

  13. Literature for Today's Young Adults. Fourth Edition.

    ERIC Educational Resources Information Center

    Nilsen, Alleen Pace; Donelson, Kenneth L.

    Designed to help teachers open young minds to literature, this book presents criteria for evaluating books in all genres and their suggested classroom uses, an examination of hotly debated topics, and an overview of the significance of young adult literature. The fourth edition of the book features 30 boxed inserts containing essays by some of the…

  14. College Student Press Law. Second Edition.

    ERIC Educational Resources Information Center

    Trager, Robert; Dickerson, Donna L.

    This second edition of a monograph provides updated information on court decisions concerning college student publications and underground newspapers to acquaint advisers, administrators, and students with college student press law. Chapters of the monograph examine freedom of speech on the college campus; the relationship between colleges and…

  15. Editing Distance Education Materials. Knowledge Series.

    ERIC Educational Resources Information Center

    Swales, Christine

    Distance education (DE) materials take a learner-centered approach rather than the traditionally content-centered approach of textbooks. This fact has several implications for the editing of DE materials. The role of the editor within the DE organization will depend on the organization's size and structure. The basic features of the DE program or…

  16. Kids & Family Reading Report™. 5th Edition

    ERIC Educational Resources Information Center

    Scholastic Inc., 2015

    2015-01-01

    This report presents the 5th Edition of Scholastic's biannual study of children's and parents' attitudes and behaviors about reading. The latest research touches on reading aloud to children of all ages, the impact of reading independently for fun at school and at home, the importance of frequent reading, and the books children want most to read.…

  17. Making Classroom Assessment Work. Third Edition

    ERIC Educational Resources Information Center

    Davies, Anne

    2011-01-01

    3rd Edition! When should we assess, and when should we evaluate? What might be the results of evaluating too early or too much? How do we know if we are evaluating the right things? How do we know what makes sense for the learner and for the course? These questions are at the heart of "Making Classroom Assessment Work." This book combines powerful…

  18. Part C Updates. 11th Edition

    ERIC Educational Resources Information Center

    Danaher, Joan; Goode, Sue; Lazara, Alex

    2010-01-01

    "Part C Updates" is a compilation of information on various aspects of the Early Intervention Program for Infants and Toddlers with Disabilities (Part C) of the Individuals with Disabilities Education Act (IDEA). This is the eleventh volume in a series of compilations, which included two editions of Part H Updates, the former name of the program.…

  19. Health Activities Project (HAP), Trial Edition II.

    ERIC Educational Resources Information Center

    Buller, Dave; And Others

    Contained within this Health Activities Project (HAP) trial edition (set II) are a teacher information folio and numerous student activity folios which center around the idea that students in grades 5-8 can control their own health and safety. Each student folio is organized into a Synopsis, Health Background, Materials, Setting Up, and Activities…

  20. Computing Services Writing and Editing Standards

    SciTech Connect

    Caruthers, C.; Heiberger, A.

    1985-08-01

    This report provides information that will enable writers to produce better documents consistent with Computing Services standards in less time. This document provides advice for organizing and writing technical information clearly and concisely, and it explains Computing Services usage standards. Applying the principles in this document will speed the writing, editing, review, and revision process in Computing Services.

  1. BOOK REVIEW: ENVIRONMENTAL ENGINEERING, 5TH EDITION

    EPA Science Inventory

    Book Review of Environmental Engineering, 5th Edition (Joseph A. Salvato, Nelson L. Nemerow, Franklin J. Agardy (Editors), John Wiley and Sons, Inc. Hoboken, New Jersey. 2003.). Author wrote review per the request of the Editor-in-Chief of the Journal of Environmental Quality.

  2. Career Choice and Development. Third Edition.

    ERIC Educational Resources Information Center

    Brown, Duane; And Others

    This book contains 12 papers examining established and newly emerging theories of career choice and development. Following prefaces to the third, second, and first editions by Duane Brown and Linda Brooks, the following papers are included: "Introduction to Theories of Career Development and Choice: Origins, Evolution, and Current Efforts" (Duane…

  3. Effective Literacy Instruction, K-8. Fourth Edition.

    ERIC Educational Resources Information Center

    Leu, Donald J., Jr.; Kinzer, Charles K.

    Systematically integrating technology with accepted practices for teaching reading in a balanced literacy education program, this book helps to develop insightful teachers empowered to make thoughtful decisions about reading instruction. Updated and extensively revised with new material throughout, this fourth edition features a balanced…

  4. How To Integrate the Curricula. Second Edition.

    ERIC Educational Resources Information Center

    Fogarty, Robin

    This second edition of a book on integrating the curriculum calls the driving force behind integrated curricula the "four winds of change": theorists, educators, parents, and students who demand a curriculum that will apply research on how the brain works, address standards, support lifelong learning, center on the student, and offer meaningful…

  5. Engineered Viruses as Genome Editing Devices.

    PubMed

    Chen, Xiaoyu; Gonçalves, Manuel A F V

    2016-03-01

    Genome editing based on sequence-specific designer nucleases, also known as programmable nucleases, seeks to modify in a targeted and precise manner the genetic information content of living cells. Delivering into cells designer nucleases alone or together with donor DNA templates, which serve as surrogate homologous recombination (HR) substrates, can result in gene knockouts or gene knock-ins, respectively. As engineered replication-defective viruses, viral vectors are having an increasingly important role as delivery vehicles for donor DNA templates and designer nucleases, namely, zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated Cas9 (CRISPR-Cas9) nucleases, also known as RNA-guided nucleases (RGNs). We review this dual role played by engineered viral particles on genome editing while focusing on their main scaffolds, consisting of lentiviruses, adeno-associated viruses, and adenoviruses. In addition, the coverage of the growing body of research on the repurposing of viral vectors as delivery systems for genome editing tools is complemented with information regarding their main characteristics, pros, and cons. Finally, this information is framed by a concise description of the chief principles, tools, and applications of the genome editing field as a whole. PMID:26336974

  6. Handbook of Infant Mental Health. Second Edition.

    ERIC Educational Resources Information Center

    Zeanah, Charles H., Jr., Ed.

    This revised edition offers an interdisciplinary analysis of the developmental, clinical, and social aspects of mental health from birth to age 3. Chapters are organized into five areas, covering the context of mental health, risk and protective factors, assessment, psychopathology, intervention, and applications of infant mental health. The…

  7. Schools without Drugs. What Works. Revised Edition.

    ERIC Educational Resources Information Center

    Department of Education, Washington, DC.

    This revised edition focuses on the prevention of drug use among school students, with increased attention to alcohol, tobacco, and steroids. The handbook, which begins with an introduction by Secretary of Education, Lauro F. Cavazos, provides new information about the effects of alcohol on young people; statistics on the harm it causes; and…

  8. Group Counseling: Concepts and Procedures. Third Edition.

    ERIC Educational Resources Information Center

    Berg, Robert C.; Landreth, Garry L.; Fall, Kevin A.

    This third edition is designed to be used as a primary source for traditional courses in group counseling. Providing a thorough discussion of the rationale for using group counseling, this book examines the differing approaches of each author to group counseling, outlines practical suggestions on the skills needed for effective facilitation of…

  9. Health Education Teaching Ideas: Secondary. Revised Edition.

    ERIC Educational Resources Information Center

    Loya, Richard, Ed.; Bensley, Loren B., Jr., Ed.

    Part I of this teaching guide contains teaching strategies which originally appeared in the "Journal of Health Education" (JHE) and were included in the first edition of this guide, published in 1983. Part II includes teaching strategies published in JHE since 1983. The guide is designed to be a reference for those seeking workable ideas in…

  10. A Model for Flexibly Editing CSCL Scripts

    ERIC Educational Resources Information Center

    Sobreira, Pericles; Tchounikine, Pierre

    2012-01-01

    This article presents a model whose primary concern and design rationale is to offer users (teachers) with basic ICT skills an intuitive, easy, and flexible way of editing scripts. The proposal is based on relating an end-user representation as a table and a machine model as a tree. The table-tree model introduces structural expressiveness and…

  11. Best Practices in Literacy Instruction. Third Edition

    ERIC Educational Resources Information Center

    Gambrell, Linda B., Ed.; Morrow, Lesley Mandel, Ed.; Pressley, Michael, Ed.; Guthrie, John T., Ed.

    2006-01-01

    Now in a thoroughly revised and expanded third edition, this evidence-based book distills the latest knowledge about literacy teaching and learning into clear strategies for helping all children succeed. Within a comprehensive conceptual framework, the field's leading authorities provide eminently practical recommendations to guide instructional…

  12. The Landscape of Qualitative Research. Third Edition

    ERIC Educational Resources Information Center

    Denzin, Norman K., Ed.; Lincoln, Yvonna, Ed.

    2007-01-01

    This book, the first volume of the paperback versions of the "The SAGE Handbook of Qualitative Research, Third Edition," takes a look at the field from a broadly theoretical perspective, and is composed of the Handbook's Parts I ("Locating the Field"), II ("Major Paradigms and Perspectives"), and VI ("The Future of Qualitative Research"). "The…

  13. Academic Skills Problems Workbook. Revised Edition

    ERIC Educational Resources Information Center

    Shapiro, Edward S.

    2004-01-01

    An ideal companion to "Academic Skills Problems, Third Edition," this user-friendly workbook offers numerous opportunities for practicing and mastering direct assessment and intervention procedures. Practice exercises and forms--some of which are reprinted from the text--are presented in a convenient 8 1/2" x 11" format with permission to…

  14. Critical Social Theories. 2nd Edition

    ERIC Educational Resources Information Center

    Agger, Ben

    2006-01-01

    Praised for its clarity and accessibility, this fully updated edition of "Critical Social Theories" presents a comprehensive analysis of leading social and cultural theories today. Diverse perspectives are addressed from feminism and cultural studies to postmodernism and critical theory. Written accessibly for students and faculty, the second…

  15. The Community College Story. Third Edition

    ERIC Educational Resources Information Center

    Vaughan, George B.

    2006-01-01

    This concise history of community colleges touches on major themes, including open access and equity, comprehensiveness, community-based philosophy, commitment to teaching, and lifelong learning. The third edition includes revised text as well as updated statistical information, time line, reading list, and Internet resources. In the more than a…

  16. Best Practices in Literacy Instruction. Second Edition.

    ERIC Educational Resources Information Center

    Morrow, Lesley Mandel, Ed.; Gambrell, Linda B., Ed.; Pressley, Michael, Ed.

    Now revised and updated, this book's second edition aims to guide teachers in providing effective, engaging literacy instruction that meets the challenges of today's legislative mandates. Identified in the book are principles of best practice that reflect cutting-edge scientific research as well as decades of hands-on classroom experience.…

  17. The staging system: Display and edit module

    NASA Technical Reports Server (NTRS)

    Edwards, E.; Bernier, L.

    1976-01-01

    The Display and Edit (D and E) Module described is one of six major modules being developed for the STAGING (STructural Analysis through Generalized INteractive Graphics) System. Several remarks are included concerning the computer environment and the architecture of the data base. The utility of this module is emphasized.

  18. Foundations of Mental Health Counseling. Second Edition.

    ERIC Educational Resources Information Center

    Weikel, William J., Ed.; Palmo, Artis J., Ed.

    The mental health counseling profession has gained increasing influence in the last 20 years. The purpose of this edited collection of articles is to chart the antecedents to, the present status of, and the future trends for this group of professionals. The book draws together historical tracings, rationales, conceptual models, and other…

  19. Business Management for Independent Schools. Fourth Edition.

    ERIC Educational Resources Information Center

    National Association of Independent Schools, Boston, MA.

    This fourth edition of a guide for independent school business managers has been produced in looseleaf format so that changes may be made promptly as decisions of regulatory bodies require modifications in current practice. Fourteen chapters are organized under three broad topic headings. Chapters in part 1, Accounting and Financial Reporting,…

  20. The High School Mathematics Library. Eighth Edition.

    ERIC Educational Resources Information Center

    Schaaf, William L.

    This eighth edition of the bibliography reflects changes in emphasis in the secondary school mathematics curriculum. The booklet is addressed primarily to high school students of all ability levels and to their mathematics teachers, and should also be of interest to students and instructors of mathematics in two-year colleges as well as librarians…

  1. Polyhedron Models for the Classroom. Second Edition.

    ERIC Educational Resources Information Center

    Wenninger, Magnus J.

    This second edition explains the historical background and techniques for constructing various types of polyhedra. Seven center-fold sheets are included, containing full-scale drawings from which nets or templates may be made to construct the models shown and described in the text. Details are provided for construction of the five Platonic solids,…

  2. Michigan Library Statistical Report, 1998 Edition.

    ERIC Educational Resources Information Center

    Krefman, Naomi, Comp.; Krueger, Beth, Comp.; Sachau, Denise, Comp.

    The 1998 edition of the Michigan Library Statistical Report presents data provided by public libraries, public library cooperatives, and those public libraries which serve as regional or subregional outlets to provide services for blind and physically handicapped patrons. The reporting year for the public libraries is the most recent fiscal year…

  3. Michigan Library Statistical Report, 2001 Edition.

    ERIC Educational Resources Information Center

    Krefman, Naomi, Comp.; Dwyer, Molly, Comp.; Wetzel, Beth, Comp.

    The 2001 edition of the "Michigan Library Statistical Report" presents data for fiscal year 2000 provided by public libraries, library cooperatives, and those public libraries that serve as regional or subregional outlets to provide services for blind and physically handicapped patrons. Statewide summaries are presented, after statistical tables…

  4. Michigan Library Statistical Report, 2000 Edition.

    ERIC Educational Resources Information Center

    Krefman, Naomi, Comp.; Dwyer, Molly, Comp.; Krueger, Beth, Comp.

    The 2000 edition of the Michigan Library Statistical Report presents data provided by public libraries, library cooperatives and those public libraries that serve as regional or subregional outlets for providing services to blind and physically handicapped patrons. All legally established public libraries in Michigan were invited to respond. Data…

  5. The Excellence of Play. Second Edition

    ERIC Educational Resources Information Center

    Moyles, Janet, Ed.

    2005-01-01

    This second edition of "The Excellence of Play" encapsulates all of the many changes that have taken place in early childhood in the last decade. It examines the vital importance of play as a tool for learning and teaching for children and practitioners, supporting all those who work in early childhood education and care in developing and…

  6. The Technique of Television Production. Revised Edition.

    ERIC Educational Resources Information Center

    Millerson, Gerald

    In discussing the technical aspects of television production, this book covers both equipment and techniques used in these areas: camera, lighting, sound, settings, and make-up. Composition of images according to camera movement, placement of subjects, editing, and aural composition are also covered. Steps in the technical planning of a telecast…

  7. World Bank Atlas. [Twenty-Fourth Edition.

    ERIC Educational Resources Information Center

    World Bank, Washington, DC.

    This edition of the World Bank Atlas presents curent economic and social data for 185 countries and territories in the world. A number of maps, tables, and graphs highlight key relationships and trends in the development of the countries. The atlas includes data on population, gross national product (GNP), share of agriculture in gross domestic…

  8. Handbook of paediatric radiography. Second edition

    SciTech Connect

    Gyll, C.

    1985-01-01

    This book gives some ideas on how to achieve good radiographs of children. In this second edition most papers are expanded and brought up to date, the paper on the neonate completely rewritten, and a discussion of child development and child psychology added.

  9. Recommended Reference Books in Paperback. Third Edition.

    ERIC Educational Resources Information Center

    Lang, Jovian P.; O'Gorman, Jack

    Completely revised and updated from the last edition (1992), this annotated, evaluative bibliography presents more than 1,000 outstanding titles chosen for their quality, economy, and availability. Thirty-six chapters describe and judge these affordable paperbacks for libraries with limited budgets. Subject matter includes: general reference, area…

  10. The Young Child and Mathematics. Second Edition

    ERIC Educational Resources Information Center

    Copley, Juanita V.

    2010-01-01

    This second edition of the bestselling book "The Young Child and Mathematics" reflects recent developments in math education in a wealth of vignettes from classrooms, activity ideas, and strategies for teaching young children about math processes and concepts. Using standards and guidelines from the National Council of Teachers of Mathematics…

  11. Powerful Designs for Professional Learning. Second Edition

    ERIC Educational Resources Information Center

    Easton, Lois Brown, Ed.

    2008-01-01

    An expanded second edition of the popular "Powerful Designs for Professional Learning" introduces new chapters on classroom walk-throughs, differentiated coaching, dialogue, and video. Written by educators who have successfully done this work, the chapters describe how 23 significant professional learning strategies work in practice, a rationale…

  12. Emotional Intelligence in Everyday Life. Second Edition

    ERIC Educational Resources Information Center

    Beck, John H., Ed.

    2006-01-01

    Since the release of the very successful first edition in 2001, the field of emotional intelligence has grown in sophistication and importance. Many new and talented researchers have come into the field and techniques in EI measurement have dramatically increased so that we now know much more about the distinctiveness and utility of the different…

  13. Nanoparticles for Site Specific Genome Editing

    NASA Astrophysics Data System (ADS)

    McNeer, Nicole Ali

    Triplex-forming peptide nucleic acids (PNAs) can be used to coordinate the recombination of short 50-60 by "donor DNA" fragments into genomic DNA, resulting in site-specific correction of genetic mutations or the introduction of advantageous genetic modifications. Site-specific gene editing in hematopoietic stem and progenitor cells (HSPCs) could result in treatment or cure of inherited disorders of the blood such as beta-thalassemia. Gene editing in HSPCs and differentiated T cells could help combat HIV/AIDs by modifying receptors, such as CCR5, necessary for R5-tropic HIV entry. However, translation of genome modification technologies to clinical practice is limited by challenges in intracellular delivery, especially in difficult-to-transfect hematolymphoid cells. In vivo gene editing could also provide novel treatment for systemic monogenic disorders such as cystic fibrosis, an autosomal recessive disorder caused by mutations in the cystic fibrosis transmembrane receptor. Here, we have engineered biodegradable nanoparticles to deliver oligonucleotides for site-specific genome editing of disease-relevant genes in human cells, with high efficiency, low toxicity, and editing of clinically relevant cell types. We designed nanoparticles to edit the human beta-globin and CCR5 genes in hematopoietic cells. We show that poly(lactic-co-glycolic acid) (PLGA) nanoparticles can delivery PNA and donor DNA for site-specific gene modification in human hematopoietic cells in vitro and in vivo in NOD-scid IL2rgammanull mice. Nanoparticles delivered by tail vein localized to hematopoietic compartments in the spleen and bone marrow of humanized mice, resulting in modification of the beta-globin and CCR5 genes. Modification frequencies ranged from 0.005 to 20% of cells depending on the organ and cell type, without detectable toxicity. This project developed highly versatile methods for delivery of therapeutics to hematolymphoid cells and hematopoietic stem cells, and will help to

  14. Variable Frequency of Plastid RNA Editing among Ferns and Repeated Loss of Uridine-to-Cytidine Editing from Vascular Plants

    PubMed Central

    Guo, Wenhu; Grewe, Felix; Mower, Jeffrey P.

    2015-01-01

    The distinct distribution and abundance of C-to-U and U-to-C RNA editing among land plants suggest that these two processes originated and evolve independently, but the paucity of information from several key lineages limits our understanding of their evolution. To examine the evolutionary diversity of RNA editing among ferns, we sequenced the plastid transcriptomes from two early diverging species, Ophioglossum californicum and Psilotum nudum. Using a relaxed automated approach to minimize false negatives combined with manual inspection to eliminate false positives, we identified 297 C-to-U and three U-to-C edit sites in the O. californicum plastid transcriptome but only 27 C-to-U and no U-to-C edit sites in the P. nudum plastid transcriptome. A broader comparison of editing content with the leptosporangiate fern Adiantum capillus-veneris and the hornwort Anthoceros formosae uncovered large variance in the abundance of plastid editing, indicating that the frequency and type of RNA editing is highly labile in ferns. Edit sites that increase protein conservation among species are more abundant and more efficiently edited than silent and non-conservative sites, suggesting that selection maintains functionally important editing. The absence of U-to-C editing from P. nudum plastid transcripts and other vascular plants demonstrates that U-to-C editing loss is a recurrent phenomenon in vascular plant evolution. PMID:25568947

  15. Cloud Properties of CERES-MODIS Edition 4 and CERES-VIIRS Edition 1

    NASA Technical Reports Server (NTRS)

    Sun-Mack, Sunny; Minnis, Patrick; Chang, Fu-Lung; Hong, Gang; Arduini, Robert; Chen, Yan; Trepte, Qing; Yost, Chris; Smith, Rita; Brown, Ricky; Chu, Churngwei; Heckert, Elizabeth; Gibson, Sharon; Heck, Patrick W.

    2015-01-01

    The Clouds and Earth's Radiant Energy System (CERES) analyzes MODerate-resolution Imaging Spectroradiometer (MODIS) data and Visible Infrared Imaging Radiometer Suite (VIIRS) to derive cloud properties that are combine with aerosol and CERES broadband flux data to create a multi-parameter data set for climate study. CERES has produced over 15 years of data from Terra and over 13 years of data from Aqua using the CERES-MODIS Edition-2 cloud retrieval algorithm. A recently revised algorithm, CERESMODIS Edition 4, has been developed and is now generating enhanced cloud data for climate research (over 10 years for Terra and 8 years for Aqua). New multispectral retrievals of properties are included along with a multilayer cloud retrieval system. Cloud microphysical properties are reported at 3 wavelengths, 0.65, 1.24, and 2.1 microns to enable better estimates of the vertical profiles of cloud water contents. Cloud properties over snow are retrieved using the 1.24-micron channel. A new CERES-VIIRS cloud retrieval package was developed for the VIIRS spectral complement and is currently producing the CERES-VIIRS Edition 1 cloud dataset. The results from CERES-MODIS Edition 4 and CERES-VIIRS Edition 1 are presented and compared with each other and other datasets, including CALIPSO, CloudSat and the CERES-MODIS Edition-2 results.

  16. Editor meets silencer: crosstalk between RNA editing and RNA interference

    PubMed Central

    Nishikura, Kazuko

    2010-01-01

    The most prevalent type of RNA editing is mediated by ADAR (adenosine deaminase acting on RNA) enzymes, which convert adenosines to inosines (a process known as A→I RNA editing) in double-stranded (ds)RNA substrates. A→I RNA editing was long thought to affect only selected transcripts by altering the proteins they encode. However, genome-wide screening has revealed numerous editing sites within inverted Alu repeats in introns and untranslated regions. Also, recent evidence indicates that A→I RNA editing crosstalks with RNA-interference pathways, which, like A→I RNA editing, involve dsRNAs. A→I RNA editing therefore seems to have additional functions, including the regulation of retrotransposons and gene silencing, which adds a new urgency to the challenges of fully understanding ADAR functions. PMID:17139332

  17. Presenting the 3rd edition of WRB

    NASA Astrophysics Data System (ADS)

    Schad, Peter

    2014-05-01

    The third edition of the international soil classification system "World Reference Base for Soil Resources" (WRB) will be presented during der 20th World Congress of Soil Science, Jeju, Korea, June 9-12. The second edition was published in 2006 and the first in 1998, which, in turn, was based on the Legends of the FAO Soil Map of the World. Now, after eight years of experience with the second edition, time was due for a revision. The major changes are: 1. The second edition had two different qualifier sequences for naming soils (IUSS Working Group WRB, 2006, update 2007) and for creating map legends (Guidelines for creating small-scale map legends using the WRB; IUSS Working Group WRB, 2010). The third edition has one sequence for both. The qualifiers for every Reference Soil Group are subdivided into a small number of main qualifiers that are ranked and a larger number of additional qualifiers that are not ranked and given in an alphabetical order. The name of a pedon must comprise all applying qualifiers. The name of a map unit comprises a specified small number of main qualifiers, depending on scale, whereas all other qualifiers are optional. 2. For some soils, problems have been reported. Albeluvisols are difficult to detect in the field and cover only small surfaces. They have been replaced by Retisols, which have a broader definition that is easier to identify in the field. 3. The use of some diagnostics was difficult. Examples are: The argic horizon had too low limit values, so we had much more soils with argic horizons than justified. The definitions of the cambic horizon and the gleyic and stagnic properties were not precise enough. Organic material, mollic and umbric horizons had an unnecessary complicated definition. 4. Some changes in the key to the Reference Soil Groups seemed to be justified. Fluvisols were moved further down, Durisols and Gypsisols switched their position, also Arenosols and Cambisols. The soils with an argic horizon were brought

  18. Catalog of Infrared Observations, Third Edition

    NASA Technical Reports Server (NTRS)

    Gezari, Daniel Y.; Schmitz, Marion; Pitts, Patricia S.; Mead, Jaylee M.

    1993-01-01

    The Far Infrared Supplement contains a subset of the data in the full Catalog of Infrared Observations (all observations at wavelengths greater than 4.6 microns). The Catalog of Infrared Observations (CIO), NASA RP-1294, is a compilation of infrared astronomical observational data obtained from an extensive literature search of scientific journals and major astronomical catalogs and surveys. The literature search is complete for years 1965 through 1990 in this Third Edition. The Catalog contains about 210,000 observations of roughly 20,000 individual sources and supporting appendices. The expanded Third Edition contains coded IRAS 4-band data for all CIO sources detected by IRAS. The appendices include an atlas of infrared source positions (also included in this volume), two bibliographies of Catalog listings, and an atlas of infrared spectral ranges. The complete CIO database is available to qualified users in printed, microfiche, and magnetic-tape formats.

  19. Transportation Energy Data Book, Edition 18

    SciTech Connect

    Davis, Stacy C.

    1998-09-01

    The Transportation Energy Data Book: Edition 18 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Transportation Technologies in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. This edition of the Data Book has 11 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 - energy Chapter 3 - emissions; Chapter 4 - transportation and the economy; Chapter 5 - highway vehicles; Chapter 6 - Light vehicles; Chapter 7 - heavy vehicles; Chapter 8 - alternative fuel vehicles; Chapter 9 - fleet vehicles; Chapter 10 - household vehicles; and Chapter 11 - nonhighway modes. The sources used represent the latest available data.

  20. Engineering Delivery Vehicles for Genome Editing.

    PubMed

    Nelson, Christopher E; Gersbach, Charles A

    2016-06-01

    The field of genome engineering has created new possibilities for gene therapy, including improved animal models of disease, engineered cell therapies, and in vivo gene repair. The most significant challenge for the clinical translation of genome engineering is the development of safe and effective delivery vehicles. A large body of work has applied genome engineering to genetic modification in vitro, and clinical trials have begun using cells modified by genome editing. Now, promising preclinical work is beginning to apply these tools in vivo. This article summarizes the development of genome engineering platforms, including meganucleases, zinc finger nucleases, TALENs, and CRISPR/Cas9, and their flexibility for precise genetic modifications. The prospects for the development of safe and effective viral and nonviral delivery vehicles for genome editing are reviewed, and promising advances in particular therapeutic applications are discussed. PMID:27146557

  1. Motion Editing for Time-Varying Mesh

    NASA Astrophysics Data System (ADS)

    Xu, Jianfeng; Yamasaki, Toshihiko; Aizawa, Kiyoharu

    2008-12-01

    Recently, time-varying mesh (TVM), which is composed of a sequence of mesh models, has received considerable interest due to its new and attractive functions such as free viewpoint and interactivity. TVM captures the dynamic scene of the real world from multiple synchronized cameras. However, it is expensive and time consuming to generate a TVM sequence. In this paper, an editing system is presented to reuse the original data, which reorganizes the motions to obtain a new sequence based on the user requirements. Hierarchical motion structure is observed and parsed in TVM sequences. Then, the representative motions are chosen into a motion database, where a motion graph is constructed to connect those motions with smooth transitions. After the user selects some desired motions from the motion database, the best paths are searched by a modified Dijkstra algorithm to achieve a new sequence. Our experimental results demonstrate that the edited sequences are natural and smooth.

  2. Radiation Therapy Physics, 3rd Edition

    NASA Astrophysics Data System (ADS)

    Hendee, William R.; Ibbott, Geoffrey S.; Hendee, Eric G.

    2004-08-01

    The Third Edition of Radiation Therapy Physics addresses in concise fashion the fundamental diagnostic radiologic physics principles as well as their clinical implications. Along with coverage of the concepts and applications for the radiation treatment of cancer patients, the authors have included reviews of the most up-to-date instrumentation and critical historical links. The text includes coverage of imaging in therapy planning and surveillance, calibration protocols, and precision radiation therapy, as well as discussion of relevant regulation and compliance activities. It contains an updated and expanded section on computer applications in radiation therapy and electron beam therapy, and features enhanced user-friendliness and visual appeal with a new, easy-to-follow format, including sidebars and a larger trim size. With its user-friendly presentation and broad, comprehensive coverage of radiotherapy physics, this Third Edition doubles as a medical text and handy professional reference.

  3. Selective access and editing in a database

    NASA Technical Reports Server (NTRS)

    Maluf, David A. (Inventor); Gawdiak, Yuri O. (Inventor)

    2010-01-01

    Method and system for providing selective access to different portions of a database by different subgroups of database users. Where N users are involved, up to 2.sup.N-1 distinguishable access subgroups in a group space can be formed, where no two access subgroups have the same members. Two or more members of a given access subgroup can edit, substantially simultaneously, a document accessible to each member.

  4. Medical Imaging Physics, 4th Edition

    NASA Astrophysics Data System (ADS)

    Hendee, William R.; Ritenour, E. Russell

    2002-05-01

    This comprehensive publication covers all aspects of image formation in modern medical imaging modalities, from radiography, fluoroscopy, and computed tomography, to magnetic resonance imaging and ultrasound. It addresses the techniques and instrumentation used in the rapidly changing field of medical imaging. Now in its fourth edition, this text provides the reader with the tools necessary to be comfortable with the physical principles, equipment, and procedures used in diagnostic imaging, as well as appreciate the capabilities and limitations of the technologies.

  5. Transportation Energy Data Book: Edition 34

    SciTech Connect

    Davis, Stacy Cagle; Williams, Susan E; Boundy, Robert Gary

    2015-08-01

    The Transportation Energy Data Book: Edition 34 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Vehicle Technologies Office. Designed for use as a desk-top reference, the Data Book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest edition of the Data Book is available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 energy; Chapter 3 highway vehicles; Chapter 4 light vehicles; Chapter 5 heavy vehicles; Chapter 6 alternative fuel vehicles; Chapter 7 fleet vehicles; Chapter 8 household vehicles; Chapter 9 nonhighway modes; Chapter 10 transportation and the economy; Chapter 11 greenhouse gas emissions; and Chapter 12 criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the reader s convenience.

  6. Transportation Energy Data Book: Edition 30

    SciTech Connect

    Davis, Stacy Cagle; Diegel, Susan W; Boundy, Robert Gary

    2011-07-01

    The Transportation Energy Data Book: Edition 30 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Vehicle Technologies Program. Designed for use as a desk-top reference, the Data Book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest edition of the Data Book is available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 energy; Chapter 3 highway vehicles; Chapter 4 light vehicles; Chapter 5 heavy vehicles; Chapter 6 alternative fuel vehicles; Chapter 7 fleet vehicles; Chapter 8 household vehicles; Chapter 9 nonhighway modes; Chapter 10 transportation and the economy; Chapter 11 greenhouse gas emissions; and Chapter 12 criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the reader s convenience.

  7. Transportation Energy Data Book: Edition 31

    SciTech Connect

    Davis, Stacy Cagle; Diegel, Susan W; Boundy, Robert Gary

    2012-08-01

    The Transportation Energy Data Book: Edition 31 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Vehicle Technologies Program. Designed for use as a desk-top reference, the Data Book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest edition of the Data Book is available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 energy; Chapter 3 highway vehicles; Chapter 4 light vehicles; Chapter 5 heavy vehicles; Chapter 6 alternative fuel vehicles; Chapter 7 fleet vehicles; Chapter 8 household vehicles; Chapter 9 nonhighway modes; Chapter 10 transportation and the economy; Chapter 11 greenhouse gas emissions; and Chapter 12 criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the reader s convenience.

  8. Transportation Energy Data Book. Edition 33

    SciTech Connect

    Davis, Stacy Cagle; Williams, Susan E.; Boundy, Robert Gary

    2014-07-01

    The Transportation Energy Data Book: Edition 33 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Vehicle Technologies Office. Designed for use as a desk-top reference, the Data Book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest edition of the Data Book is available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 energy; Chapter 3 highway vehicles; Chapter 4 light vehicles; Chapter 5 heavy vehicles; Chapter 6 alternative fuel vehicles; Chapter 7 fleet vehicles; Chapter 8 household vehicles; Chapter 9 nonhighway modes; Chapter 10 transportation and the economy; Chapter 11 greenhouse gas emissions; and Chapter 12 criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the reader s convenience.

  9. Transportation Energy Data Book: Edition 32

    SciTech Connect

    Davis, Stacy Cagle; Diegel, Susan W; Boundy, Robert Gary

    2013-08-01

    The Transportation Energy Data Book: Edition 32 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Vehicle Technologies Office. Designed for use as a desk-top reference, the Data Book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest edition of the Data Book is available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 energy; Chapter 3 highway vehicles; Chapter 4 light vehicles; Chapter 5 heavy vehicles; Chapter 6 alternative fuel vehicles; Chapter 7 fleet vehicles; Chapter 8 household vehicles; Chapter 9 nonhighway modes; Chapter 10 transportation and the economy; Chapter 11 greenhouse gas emissions; and Chapter 12 criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the reader s convenience.

  10. Transportation Energy Data Book: Edition 28

    SciTech Connect

    Davis, Stacy Cagle; Diegel, Susan W; Boundy, Robert Gary

    2009-06-01

    The Transportation Energy Data Book: Edition 28 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with U.S Department of Energy, Office of Energy Efficiency and Renewable Energy, Vehicle Technologies Program and the Hydrogen, Fuel Cells, and Infrastructure Technologies Program. Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest edition of the Data Book are available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 energy; Chapter 3 highway vehicles; Chapter 4 light vehicles; Chapter 5 heavy vehicles; Chapter 6 alternative fuel vehicles; Chapter 7 fleet vehicles; Chapter 8 household vehicles; and Chapter 9 nonhighway modes; Chapter 10 transportation and the economy; Chapter 11 greenhouse gas emissions; and Chapter 12 criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the readers convenience.

  11. Transportation Energy Data Book: Edition 29

    SciTech Connect

    Davis, Stacy Cagle; Diegel, Susan W; Boundy, Robert Gary

    2010-07-01

    The Transportation Energy Data Book: Edition 29 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Vehicle Technologies Program. Designed for use as a desk-top reference, the Data Book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest edition of the Data Book is available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 energy; Chapter 3 highway vehicles; Chapter 4 light vehicles; Chapter 5 heavy vehicles; Chapter 6 alternative fuel vehicles; Chapter 7 fleet vehicles; Chapter 8 household vehicles; Chapter 9 nonhighway modes; Chapter 10 transportation and the economy; Chapter 11 greenhouse gas emissions; and Chapter 12 criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the reader s convenience.

  12. Transportation Energy Data Book: Edition 27

    SciTech Connect

    Davis, Stacy Cagle; Diegel, Susan W; Boundy, Robert Gary

    2008-06-01

    The Transportation Energy Data Book: Edition 27 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Planning, Budget Formulation, and Analysis, under the Energy Efficiency and Renewable Energy (EERE) program in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest editions of the Data Book are available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 energy; Chapter 3 highway vehicles; Chapter 4 light vehicles; Chapter 5 heavy vehicles; Chapter 6 alternative fuel vehicles; Chapter 7 fleet vehicles; Chapter 8 household vehicles; and Chapter 9 nonhighway modes; Chapter 10 transportation and the economy; Chapter 11 greenhouse gas emissions; and Chapter 12 criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the readers convenience.

  13. Transportation Energy Data Book (Edition 20)

    SciTech Connect

    Davis, S.C.

    2000-10-09

    The ''Transportation Energy Data Book: Edition 20'' is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Transportation Technologies in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest editions of the Data Book are available to a larger audience via the Internet (www-cta.ornl.gov/data/tedb.htm). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2--energy; Chapter 3--greenhouse gas emissions; Chapter 4--criteria pollutant emissions; Chapter 5--transportation and the economy; Chapter 6--highway vehicles; Chapter 7--light vehicles; Chapter 8--heavy vehicles; Chapter 9--alternative fuel vehicles; Chapter 10--fleet vehicles; Chapter 11--household vehicles; and Chapter 12--nonhighway modes. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the readers convenience.

  14. Transportation Energy Data Book: Edition 25

    SciTech Connect

    Davis, Stacy Cagle; Diegel, Susan W

    2006-06-01

    The Transportation Energy Data Book: Edition 25 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Planning, Budget Formulation, and Analysis, under the Energy Efficiency and Renewable Energy (EERE) program in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest editions of the Data Book are available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 - energy; Chapter 3 - highway vehicles; Chapter 4 - light vehicles; Chapter 5 - heavy vehicles; Chapter 6 - alternative fuel vehicles; Chapter 7 - fleet vehicles; Chapter 8 - household vehicles; and Chapter 9- nonhighway modes; Chapter 10 - transportation and the economy; Chapter 11 - greenhouse gas emissions; and Chapter 12 - criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the readers convenience.

  15. Transportation Energy Data Book: Edition 24

    SciTech Connect

    Davis, S.C.

    2005-03-08

    The ''Transportation Energy Data Book: Edition 24'' is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Planning, Budget Formulation, and Analysis, under the Energy Efficiency and Renewable Energy (EERE) program in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest editions of the Data Book are available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2--energy; Chapter 3--highway vehicles; Chapter 4--light vehicles; Chapter 5--heavy vehicles; Chapter 6--alternative fuel vehicles; Chapter 7--fleet vehicles; Chapter 8--household vehicles; and Chapter 9--nonhighway modes; Chapter 10--transportation and the economy; Chapter 11--greenhouse gas emissions; and Chapter 12--criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the readers convenience.

  16. Transportation Energy Data Book: Edition 21

    SciTech Connect

    Davis, S.C.

    2001-09-13

    The ''Transportation Energy Data Book: Edition 21'' is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Transportation Technologies in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest editions of the Data Book are available to a larger audience via the Internet (www-cta.ornl.gov/data/tedb.htm). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2--energy; Chapter 3--greenhouse gas emissions; Chapter 4--criteria pollutant emissions; Chapter 5--transportation and the economy; Chapter 6--highway vehicles; Chapter 7--light vehicles; Chapter 8--heavy vehicles; Chapter 9--alternative fuel vehicles; Chapter 10--fleet vehicles; Chapter 11--household vehicles; and Chapter 12--nonhighway modes. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the readers convenience.

  17. Transportation Energy Data Book: Edition 23

    SciTech Connect

    Davis, S.C.

    2003-10-24

    The ''Transportation Energy Data Book: Edition 23'' is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Planning, Budget Formulation, and Analysis, under the Energy Efficiency and Renewable Energy (EERE) program in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest editions of the Data Book are available to a larger audience via the Internet (www-cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2--energy; Chapter 3--highway vehicles; Chapter 4--light vehicles; Chapter 5--heavy vehicles; Chapter 6--alternative fuel vehicles; Chapter 7--fleet vehicles; Chapter 8--household vehicles; and Chapter 9--nonhighway modes; Chapter 10--transportation and the economy; Chapter 11--greenhouse gas emissions; and Chapter 12--criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the readers convenience.

  18. Transportation Energy Data Book: Edition 26

    SciTech Connect

    Davis, Stacy Cagle; Diegel, Susan W

    2007-07-01

    The Transportation Energy Data Book: Edition 26 is a statistical compendium prepared and published by Oak Ridge National Laboratory (ORNL) under contract with the Office of Planning, Budget Formulation, and Analysis, under the Energy Efficiency and Renewable Energy (EERE) program in the Department of Energy (DOE). Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. The latest editions of the Data Book are available to a larger audience via the Internet (cta.ornl.gov/data). This edition of the Data Book has 12 chapters which focus on various aspects of the transportation industry. Chapter 1 focuses on petroleum; Chapter 2 - energy; Chapter 3 - highway vehicles; Chapter 4 - light vehicles; Chapter 5 - heavy vehicles; Chapter 6 - alternative fuel vehicles; Chapter 7 - fleet vehicles; Chapter 8 - household vehicles; and Chapter 9- nonhighway modes; Chapter 10 - transportation and the economy; Chapter 11 - greenhouse gas emissions; and Chapter 12 - criteria pollutant emissions. The sources used represent the latest available data. There are also three appendices which include detailed source information for some tables, measures of conversion, and the definition of Census divisions and regions. A glossary of terms and a title index are also included for the readers convenience.

  19. Towards GERB Edition 2 TOA fluxes

    NASA Astrophysics Data System (ADS)

    Ipe, Alessandro; Baudrez, Edward; Clerbaux, Nicolas; Moreels, Johan; Urbain, Manon; Velazquez Blazquez, Almudena

    2016-04-01

    The Geostationary Earth Radiation Budget (GERB) dataset currently covers more than 10 years from 2004 and makes it an unique record for the climate and the numerical weather prediction scientific communities through assimilation in various models and climate studies. Indeed, the geostationary platform of this broadband radiometer flying together with the Spinning Enhanced Visible and InfraRed Imager (SEVIRI) on board of the Meteosat Second Generation (MSG) satellites allows to estimate TOA solar and thermal fluxes every 15 minutes at spatial resolutions upto 10 km (nadir). In this contribution, we will discuss the improvements that were developped for the Edition 1 post-processing. These includes terminator and sunglint modeling through scene identification extrapolation. Moreover, with the experience acquired by generating the Edition 1 dataset as well as through its critical assessment, an improved Edition 2 of the processing is been implemented. This second version aims to fulfill climate data record standards. Such goal will be achieved by improving the scene identification for the selection of solar angular dependency models (ADMs), the solar and thermal narrow-to-broadband conversion schemes, as well as including new thermal ADMs for radiance-to-flux conversion and GERB instrument ageing correction schemes.

  20. Genetic map of Salmonella typhimurium, edition VIII.

    PubMed Central

    Sanderson, K E; Hessel, A; Rudd, K E

    1995-01-01

    We present edition VIII of the genetic map of Salmonella typhimurium LT2. We list a total of 1,159 genes, 1,080 of which have been located on the circular chromosome and 29 of which are on pSLT, the 90-kb plasmid usually found in LT2 lines. The remaining 50 genes are not yet mapped. The coordinate system used in this edition is neither minutes of transfer time in conjugation crosses nor units representing "phage lengths" of DNA of the transducing phage P22, as used in earlier editions, but centisomes and kilobases based on physical analysis of the lengths of DNA segments between genes. Some of these lengths have been determined by digestion of DNA by rare-cutting endonucleases and separation of fragments by pulsed-field gel electrophoresis. Other lengths have been determined by analysis of DNA sequences in GenBank. We have constructed StySeq1, which incorporates all Salmonella DNA sequence data known to us. StySeq1 comprises over 548 kb of nonredundant chromosomal genomic sequences, representing 11.4% of the chromosome, which is estimated to be just over 4,800 kb in length. Most of these sequences were assigned locations on the chromosome, in some cases by analogy with mapped Escherichia coli sequences. PMID:7603411

  1. Genome Editing in Human Pluripotent Stem Cells.

    PubMed

    Smith, Cory; Ye, Zhaohui; Cheng, Linzhao

    2016-01-01

    Pluripotent stem cells (PSCs), defined by their capacity for self-renewal and differentiation into all cell types, are an integral tool for basic biological research and disease modeling. However, full use of PSCs for research and regenerative medicine requires the ability to precisely edit their DNA to correct disease-causing mutations and for functional analysis of genetic variations. Recent advances in DNA editing of human stem cells (including PSCs) have benefited from the use of designer nucleases capable of making double-strand breaks (DSBs) at specific sequences that stimulate endogenous DNA repair. The clustered, regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has become the preferred designer nuclease for genome editing in human PSCs and other cell types. Here we describe the principles for designing a single guide RNA to uniquely target a gene of interest and describe strategies for disrupting, inserting, or replacing a specific DNA sequence in human PSCs. The improvements in efficiency and ease provided by these techniques allow individuals to precisely engineer PSCs in a way previously limited to large institutes and core facilities. PMID:27037079

  2. TALEN gene editing takes aim on HIV.

    PubMed

    Benjamin, Ronald; Berges, Bradford K; Solis-Leal, Antonio; Igbinedion, Omoyemwen; Strong, Christy L; Schiller, Martin R

    2016-09-01

    Transcription activator-like effector nucleases (TALENs) are one of several types of programmable, engineered nucleases that bind and cleave specific DNA sequences. Cellular machinery repairs the cleaved DNA by introducing indels. In this review, we emphasize the potential, explore progress, and identify challenges in using TALENs as a therapeutic tool to treat HIV infection. TALENs have less off-target editing and can be more effective at tolerating HIV escape mutations than CRISPR/Cas-9. Scientists have explored TALEN-mediated editing of host genes such as viral entry receptors (CCR5 and CXCR4) and a protein involved in proviral integration (LEDGF/p75). Viral targets include the proviral DNA, particularly focused on the long terminal repeats. Major challenges with translating gene therapy from bench to bedside are improving cleavage efficiency and delivery, while minimizing off-target editing, cytotoxicity, and immunogenicity. However, rapid improvements in TALEN technology are enhancing cleavage efficiency and specificity. Therapeutic testing in animal models of HIV infection will help determine whether TALENs are a viable HIV treatment therapy. TALENs or other engineered nucleases could shift the therapeutic paradigm from life-long antiretroviral therapy toward eradication of HIV infection. PMID:27170155

  3. The levels of edit. [technical writing in science

    NASA Technical Reports Server (NTRS)

    Vanburen, R.; Buehler, M. F.; Wallenbrock, D. (Editor)

    1976-01-01

    The editorial process is analyzed, and five levels of edit are identified. These levels represent cumulative combinations of nine types of edit: (1) coordination, (2) policy, (3) integrity, (4) screening, (5) copy clarification, (6) Mechanical Style, (7) Language, and (9) substantive. The levels and types of edit, although developed for specific use with external reports at the Jet Propulsion Laboratory, cover the general range of technical editing, especially as it applies to an in-house technical publications organization. Each type of edit is set forth in terms of groups of actions to be performed by the editor. The edit-level concept has enhanced understanding and communication among editors, authors, and publications managers concerning the specific editorial work to be done on each manuscript. It has also proved useful as a management tool for estimating and monitoring cost.

  4. Functions and Regulation of RNA Editing by ADAR Deaminases

    PubMed Central

    Nishikura, Kazuko

    2010-01-01

    One type of RNA editing converts adenosines to inosines (A→I editing) in double-stranded RNA (dsRNA) substrates. A→I RNA editing is mediated by adenosine deaminase acting on RNA (ADAR) enzymes. A→I RNA editing of protein-coding sequences of a limited number of mammalian genes results in recoding and subsequent alterations of their functions. However, A→I RNA editing most frequently targets repetitive RNA sequences located within introns and 5′ and 3′ untranslated regions (UTRs). Although the biological significance of noncoding RNA editing remains largely unknown, several possibilities, including its role in the control of endogenous short interfering RNAs (esiRNAs), have been proposed. Furthermore, recent studies have revealed that the biogenesis and functions of certain microRNAs (miRNAs) are regulated by the editing of their precursors. Here, I review the recent findings that indicate new functions for A→I editing in the regulation of noncoding RNAs and for interactions between RNA editing and RNA interference mechanisms. PMID:20192758

  5. Editing of plastid RNA in Arabidopsis thaliana ecotypes.

    PubMed

    Tillich, Michael; Funk, Helena T; Schmitz-Linneweber, Christian; Poltnigg, Peter; Sabater, Bartolomé; Martin, Mercedes; Maier, Rainer M

    2005-09-01

    Post-transcriptional maturation of plastid-encoded mRNAs from land plants includes editing by making cytidine to uridine alterations at highly specific positions; this usually restores codon identities for conserved amino acids that are important for the proper function of the affected proteins. In contrast to the rather constant number of editing sites their location varies greatly, even between closely related taxa. Here, we experimentally determined the specific pattern of editing sites (the editotype) of the plastid genome of Arabidopsis thaliana ecotype Columbia (Col-0). Based on phylogenetic analyses of plastid open reading frames, we identified 28 editing sites. Two editing events in the genes matK and ndhB seem to have evolved late during the evolution of flowering plants. Strikingly, they are embedded in almost identical sequence elements and seem to be phylogenetically co-processed. This suggests that the two sites are recognized by the same trans-factor, which could help to explain the hitherto enigmatic gain of editing sites in evolution. In order to trace variations in editotype at the subspecies level we examined two other A. thaliana accessions, Cape Verde Islands (Cvi-0) and Wassilewskija (Ws-2), for the Col-0 editing sites. Both Cvi-0 and Ws-2 possess and process the whole set of editing sites as determined in Col-0, but the consequences of RNA editing differ at one position between the ecotypes. PMID:16115067

  6. DNA-free genome editing methods for targeted crop improvement.

    PubMed

    Kanchiswamy, Chidananda Nagamangala

    2016-07-01

    Evolution of the next-generation clustered, regularly interspaced, short palindromic repeat/Cas9 (CRISPR/Cas9) genome editing tools, ribonucleoprotein (RNA)-guided endonuclease (RGEN) RNPs, is paving the way for developing DNA-free genetically edited crop plants. In this review, I discuss the various methods of RGEN RNPs tool delivery into plant cells and their limitations to adopt this technology to numerous crop plants. Furthermore, focus is given on the importance of developing DNA-free genome edited crop plants, including perennial crop plants. The possible regulation on the DNA-free, next-generation genome-edited crop plants is also highlighted. PMID:27100964

  7. Measuring Changes in Salaries and Wages in Public Schools: 2000 Edition. 27th Annual Edition.

    ERIC Educational Resources Information Center

    Williams, Alicia R.; Cooke, Willa D.; Davis, Andrea M.; Miller, Oronde A.; Lewis, JoAnn

    This annual survey reports comparable salary data for 22 professional positions. The purpose of this edition is to aid in the analysis of trends in average salaries and wages paid public-school employees in the six component groups of school personnel over the previous 10 years. This report shows how the Composite Indicator of Changes (CIC) in…

  8. Anatomy and Physiology. Module Set II: Major Body Systems. Teacher Edition [and] Student Edition. Surgical Technology.

    ERIC Educational Resources Information Center

    Hilley, Robert

    This document, which is the second part of a two-part set of modules on anatomy and physiology for future surgical technicians, contains the teacher and student editions of an introduction to anatomy and physiology that consists of modules on the following body systems: integumentary system; skeletal system; muscular system; nervous system;…

  9. Surgical application of lasers. 2nd edition

    SciTech Connect

    Dixon, J.A.

    1987-01-01

    Lasers have been successfully used in several new clinical areas such as cardiovascular, orthopedic, and pulmonary surgery as well as in specialties covered in the first edition including otorhinolaryngology, dermatology and plastic surgery, gastroenterology, and urology. These advances are all discussed in this text. Introductory chapters cover the background of laser surgery, techniques and instrumentation and safety procedures. The remaining chapters cover lasers in specific fields such as endoscopic surgery, gynecology, neurosurgery and many more. The final chapters provide an overview of photodynamic therapy and the future of laser surgery.

  10. Emerging technologies planning guide, 1993 edition

    SciTech Connect

    Not Available

    1992-11-01

    Information system technology enhancements during the next five years are expected to provide some of the most significant individual and organization work improvements ever made in the office environment. This guide is an aid to planning these technologies and assessing their roles in improving the effectiveness of Headquarters programs. Their implementation will cost-effectively support Departmental operations and the National Energy Strategy. At the hear of this process is an understanding of the relationship which exists between technology introduction and the planning, budgeting and acquisition process. The 1993 edition of this guide covers the 1993--1997 time frame.

  11. Oilfields of the World. Third edition

    SciTech Connect

    Tiratsoo, E.N.

    1985-01-01

    This third edition (updated to 1984) covers all of the world's major producing areas (both onshore and offshore) on six continents. It offers essential geologic, reserves, and production data on 13 nations that have become commercial oil producers in the last five years: Benin, Cameroon, Congo Republic, Ghana, Ivory Coast, Sudan, Zaire, Greece, The Phillippines, Sharjah, Thailand, Guatemala, and Surinam. Numerous maps display the geologic details of each area. This book also contains full-color maps of the oil and gas fields of the North Sea, Persian Gulf, Mexico, Venezuela, and Brazil.

  12. Color and Light in Nature, Second Edition

    NASA Astrophysics Data System (ADS)

    Lynch, David K.; Livingston, William

    This beautiful and informative guide provides clear explanations of all naturally occurring optical phenomena seen with the naked eye, including shadows, halos, water optics, eclipses and mirages. Separating myth from reality, it outlines the basic principles involved, and supports them with many figures and references, and a wealth of rare and spectacular photographs, many in full color, illustrates the phenomena throughout. In this new edition the authors have added over 50 new color images and provide new material on experiments readers can conduct themselves, such as how to photograph geostationary satellites with your own camera.

  13. Viewing and editing assembled sequences using Consed.

    PubMed

    Gordon, David

    2003-08-01

    Consed is a program for viewing and editing assemblies prepared by the Phrap assembly program. This unit presents a general introduction to Consed. It also provides instructions for identifying problem regions and searching in Consed. Additional protocols describe creating tags, picking primers, correcting joins and adding and removing reads. Instructions are also given for the use of Assembly View, predicting the results of a restriction digestion, the use of Consed in conjunction with PolyPhred, and using Autofinish, as well as a procedure for running Phred and Phrap. PMID:18428695

  14. Multifrequency Catalogue of Blazars - 5th Edition

    NASA Astrophysics Data System (ADS)

    Massaro, E.; Maselli, A.; Leto, C.; Marchegiani, P.; Perri, M.; Giommi, P.; Piranomonte, S.

    2014-12-01

    The 5th Edition of the Multifrequency Catalogue of Blazars is one of the most complete lists of Active Galactic Nuclei whose emission properties are recognised as typical of blazars. It includes the list of sources and an essential compilation of multifrequency data from radio to gamma rays. The source list for the entire sky is also available online at the ASDC web site (http://www.asdc.asi.it/bzcat/) where it is frequently updated to add new blazars and to improve the database.

  15. Earth's Dynamic Systems, 8th Edition

    NASA Astrophysics Data System (ADS)

    Winterer, Edward L.

    From the very first edition, Hamblin and Christiansen's elementary geology text, Earth's Dynamic Systems, has stood above its competitors in the quality of its illustrations— all of them now in color. These are exceptionally well planned to bring out essential points of the text and are models of clarity and artistic design, especially the three-dimensional cutaway diagrams of tectonic and geomorphic features. Many new drawings and photos have been incorporated in this 8th edition, including dramatic pictures of planetary surfaces. Each of the 25 chapters begins with an opening statement that puts the chapter in a larger context and closes with a list of key terms, some thought-provoking review questions, a list of suggested readings in more advanced works, and—a novel and useful feature—a list of Web sites germane to the chapter. An illustrated glossary and a useful index complete the book. Pages feature wake-up questions for the student, such as “How do we know that streams erode the valleys through which they flow?”

  16. DNA evolution and successive file editions

    NASA Astrophysics Data System (ADS)

    Zebende, Gilney F.; Penna, Thadeu J. P.; Oliveira, Paulo Murilo C. de

    Sequences of nucleotides along DNA chains are known to present long range correlations. These correlations are small for simple species (algae) and increase for more complex ones. Scanning DNA chains one finds pieces called exons which are known to code some protein sequence, and others called introns whose usefulness is debatable and do not code protein sequences. By reading only exons (skipping introns), one always gets no correlation at all, in spite of observing a large correlation by reading the whole DNA sequence. The proposed explanation is that introns are fossil DNA parts no longer in use after evolutional replacement by new, better material (current exons). Sucessive editions of the files stored in a diskette follow the same dynamic mechanism proposed for DNA evolution. Current versions of the files play the role of exons, whereas introns correspond to old versions no longer in use (but still partially stored on the disk). We find that correlations indeed increase as more and more editions are performed. This artificial system has the advantage, over real DNA data, of allowing experiments.

  17. Handbook of corrosion data, 2nd edition

    SciTech Connect

    Craig, B.; Anderson, D.

    1995-12-31

    As in the prior edition, in one convenient volume this book makes it easy to find what effect environment has on the corrosion of metals and alloys. Coverage on all the environments in the first edition has been updated and expanded and some 80 or more environments have been added, including food products (chocolate, milk, cider, beer, etc.), fruit juices (grape, pineapple, lemon, etc.), soil, blood, gasoline, fertilizers, etc. Presentation of the tabular information for all environments has been standardized throughout the book. The environments are listed alphabetically. Each listing includes a general description of the conditions, a comment on the corrosion characteristics of various alloys in such a situation, a bibliography of recent articles specific to the environment, tables consolidating and comparing corrosion rates at various temperatures and concentrations for various alloys, and graphical information. also included are summaries on the general corrosion characteristics of major metals and alloys. This separate section of the book considers each material group, such as aluminum, stainless steel, zinc and so forth. Additional tables are presented here to give the corrosion characteristics of various alloys in hundreds of environments.

  18. Digital In, Digital Out: Digital Editing with Firewire.

    ERIC Educational Resources Information Center

    Doyle, Bob; Sauer, Jeff

    1997-01-01

    Reviews linear and nonlinear digital video (DV) editing equipment and software, using the IEEE 1394 (FireWire) connector. Includes a chart listing specifications and rating eight DV editing systems, reviews two DV still-photo cameras, and previews beta DV products. (PEN)

  19. Recollection Rejection: How Children Edit Their False Memories.

    ERIC Educational Resources Information Center

    Brainerd, C. J.; Reyna, V. F.

    2002-01-01

    Presents new measure of children's use of an editing operation that suppresses false memories by accessing verbatim traces of true events. Application of the methodology showed that false-memory editing increased dramatically between early and middle childhood. Measure reacted appropriately to experimental manipulations. Developmental reductions…

  20. Foundations of Psychological Testing: A Practical Approach. Second Edition

    ERIC Educational Resources Information Center

    McIntire, Sandra A.; Miller, Leslie A.

    2006-01-01

    The second edition of "Foundations of Psychological Testing: A Practical Approach" is a text for undergraduate students new to the field of psychological testing. Using a conversational format, the authors aim to prepare students to be informed consumers as test users or test takers. Features new to the second edition include: (1) New Content; (2)…

  1. The Best of "The Running Record." Revised Edition.

    ERIC Educational Resources Information Center

    Reading Recovery Council of North America, Columbus, OH.

    This revised edition of the first volume of the "Best of the Running Record Newsletter" contains 23 articles published between March 1989 and Spring 1998--some selections are from the now out-of-print first edition. Articles are arranged by subject matter to assist the reader in finding articles which address a particular point of interest.…

  2. Invitations to Science Inquiry. Supplement to First & Second Edition.

    ERIC Educational Resources Information Center

    Liem, Tik L.

    This supplement to the first and second editions of "Invitations to Science Inquiry" contains about 100 more discrepant events (occurrences or happenings which go against what we usually think likely) presented in the second edition plus 50 additional discrepant events. Discrepant events function by causing dissonance between what is physically…

  3. Helping Children through Books: A Selected Booklist. Third Revised Edition.

    ERIC Educational Resources Information Center

    Pearl, Patricia

    An update of a bibliotherapy bibliography compiled by the Church and Synagogue Library Association (CSLA) a decade ago, this list includes books intended for children from a preschool to a sixth-grade reading level. Although the first edition included works concerning religion, this edition does not, since those works are already covered in other…

  4. An Introduction to Music Therapy: Theory and Practice. Third Edition

    ERIC Educational Resources Information Center

    Davis, William B.; Gfeller, Kate E.; Thaut, Michael H.

    2008-01-01

    "An Introduction to Music Therapy: Theory and Practice, Third Edition," provides a comprehensive overview of the practice of music therapy for the 21st century. It looks at where we have been, where we are today, and where we might be in the future. Combining sound pedagogy with recent research findings, this new edition has been updated and…

  5. Supervision: A Guide to Instructional Leadership-2nd Edition

    ERIC Educational Resources Information Center

    Burke, Peter J.; Krey, Robert D.

    2005-01-01

    The first edition of this book, titled "A Design for Instructional Supervision", provided a structural framework for an effective program of instructional supervision. The basic cognitive thrust of this second edition, "Supervision: A Guide to Instructional Leadership", remains the same as the first. What has changed is the attention to the…

  6. Classroom Discourse: The Language of Teaching and Learning. Second Edition.

    ERIC Educational Resources Information Center

    Cazden, Courtney B.

    Thirteen years after the first edition of this book, significant changes in social and intellectual life have made the subject of classroom discourse more important than ever, and this new edition integrates current perspectives and research. New features in the book include: a new rationale for the importance of student-teacher talk (the…

  7. Test Scores and What They Mean. Sixth Edition.

    ERIC Educational Resources Information Center

    Lyman, Howard B.

    The first edition of this book was written to give information about testing to people whose work gave them access to test results, but whose training included little or nothing about the use and interpretation of tests. Later editions have been intended for a broader audience as the need for understanding what test scores really mean has…

  8. Compendium of Wheat Diseases and Pests, Third Edition

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Compendium of Wheat Diseases and Pests, Third Edition, is a practical guidebook for the identification and management of over 150 important diseases, insects, and other disorders of wheat. Over 70 expert authors contributed diagnostic photographs and authoritative chapters to this edition. For e...

  9. Peace and World Order Studies: A Curriculum Guide. Fourth Edition.

    ERIC Educational Resources Information Center

    Wien, Barbara J., Ed.

    The fourth edition of this curriculum guide will help college, university, and secondary school educators design and update courses, familiarize themselves with new literature and resources, and plan and justify new academic programs in the study of global problems. While syllabus categories remain the same as in previous editions, several new…

  10. OCME: Out-of-Core Mesh Editing made practical.

    PubMed

    Ganovelli, Fabio; Scopigno, Roberto

    2012-01-01

    OCME (Out-of-Core Mesh Editing) comprises a novel data structure and related algorithms for out-of-core editing of large meshes. Triangles are inserted in a multigrid on the basis of their size in average constant time. OCME maintains no explicit hierarchy, so inserting, modifying, or deleting data doesn't require costly refitting. PMID:24806001

  11. The Handbook of Literacy Assessment and Evaluation. Second Edition.

    ERIC Educational Resources Information Center

    Harp, Bill

    This handbook gives teachers, reading specialists, administrators, or students concise, up-to-date information on the most popular assessment and evaluation tools in literacy. This second edition retains many of the tools reviewed in the first edition and adds 12 new tools. The first section reviews 24 tools that are teacher-made. The second…

  12. Annual Editions: Early Childhood Education 06/07

    ERIC Educational Resources Information Center

    Paciorek, Karen Menke, Ed.

    2006-01-01

    This 27th edition of "Annual Editions: Early Childhood Education" provides convenient, inexpensive access to current articles selected from the best of the public press. Organizational features include: an annotated listing of selected World Wide Web sites; an annotated table of contents; a topic guide; a general introduction; brief overviews for…

  13. Problem-Solving Strategies for Writing. Third Edition.

    ERIC Educational Resources Information Center

    Flower, Linda

    Reflecting new development in the field of rhetoric and composition, this textbook's third edition incorporates major changes which propose to turn theory into practical advice. These additions in the third edition draw on a new theoretical understanding of how writers operate within a discourse community, on a new research-based view of the…

  14. Read and Respond: A Text/Anthology. Third Edition.

    ERIC Educational Resources Information Center

    Swinton, Janet R.; Agopsowicz, William J.

    Retaining the feature from earlier editions that student comprehension is assessed through the writing of summaries rather than from objective test questions, this book, a combination text and anthology, is designed to improve students' reading and writing skills. New features for the third edition of this book include a new chapter on studying…

  15. Curriculum Based Assessment: A Primer. 3rd Edition

    ERIC Educational Resources Information Center

    Hargis, Charles H.

    2004-01-01

    The use of curriculum based assessment (CBA) to ensure learning disabled and low achieving students adequate educational opportunity remains the focus in this direct and comprehensive third edition. The additions to this edition are in the way of providing detail and explanation in the context of current and emerging issues in educational…

  16. 76 FR 68740 - Trademark Manual of Examining Procedure, Eighth Edition

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-07

    ... Patent and Trademark Office Trademark Manual of Examining Procedure, Eighth Edition AGENCY: United States... edition of the Trademark Manual of Examining Procedure (``TMEP''), and made available an archived copy of..., Alexandria, VA 22313-1451, marked to the attention of Editor, Trademark Manual of Examining Procedure, or...

  17. 76 FR 38639 - Trademark Board Manual of Procedure, Third Edition

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ... United States Patent and Trademark Office Trademark Board Manual of Procedure, Third Edition AGENCY... and Trademark Office (``USPTO'') issued the third edition of the Trademark Board Manual of Procedure... Judge and Editor, Trademark Board Manual of Procedure, or by hand delivery to the Trademark...

  18. 75 FR 69921 - Trademark Manual of Examining Procedure, Seventh Edition

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... Patent and Trademark Office Trademark Manual of Examining Procedure, Seventh Edition AGENCY: United... Trademark Office (``USPTO'') issued the seventh edition of the Trademark Manual of Examining Procedure... to the attention of Editor, Trademark Manual of Examining Procedure, or by hand delivery to...

  19. Textual Challenges: A Brief Guide to Choosing Shakespearean Editions

    ERIC Educational Resources Information Center

    Cornell, Christine; Malcolmson, Patrick

    2012-01-01

    How should educators go about selecting appropriate editions of Shakespeare's plays for use in political science courses? Shakespeare is turning up on many politics syllabi, but, at times, the editions chosen seem to reflect primarily a concern for price or publisher reputation over pedagogical and scholarly considerations. This article offers an…

  20. Qualitative Data Analysis: A Methods Sourcebook. Third Edition

    ERIC Educational Resources Information Center

    Miles, Matthew B.; Huberman, A. Michael; Saldana, Johnny

    2014-01-01

    The Third Edition of Miles & Huberman's classic research methods text is updated and streamlined by Johnny Saldaña, author of "The Coding Manual for Qualitative Researchers." Several of the data display strategies from previous editions are now presented in re-envisioned and reorganized formats to enhance reader accessibility and…

  1. Technical Editing as Quality Assurance: Adding Value to Content.

    ERIC Educational Resources Information Center

    Corbin, Michelle; Moell, Pat; Boyd Mike

    2002-01-01

    Reviews typical software testing activities and then compares those activities to technical editing activities. Shows that by providing quality assurance through content editing, technical editors add value to the information development process and help give users the quality content they deserve. Concludes that product information must go…

  2. Elementary Teachers Guide to Free Curriculum Materials. Thirtieth Edition.

    ERIC Educational Resources Information Center

    Suttles, Patricia H., Ed.

    The thirtieth annual edition of the guide lists 1,705 free curriculum materials, 959 new in this edition, which are selected to help elementary school teachers and librarians to choose current and relevant materials which encourage the student to inquire, to discover, and to develop the ideals that will enable him to function effectively in…

  3. Conversion Tables. Volume 2, Dewey-LC. Second Edition.

    ERIC Educational Resources Information Center

    Scott, Mona L.

    This volume contains tables that list Dewey Decimal classification numbers (i.e., call numbers) with corresponding Library of Congress classification numbers and related Library of Congress subject headings. Materials referenced in the conversion tables are the 21st edition of the "Dewey Decimal Classification", the most current edition of the…

  4. Conversion Tables. Volume 1, LC-Dewey. Second Edition.

    ERIC Educational Resources Information Center

    Scott, Mona L.

    This volume contains tables that list Library of Congress classification numbers (i.e., call numbers) with corresponding Dewey Decimal classification numbers and related Library of Congress subject headings. Materials referenced in the conversion tables are the 21st edition of the "Dewey Decimal Classification," the most current edition of the…

  5. The NMC Horizon Report: 2015 Higher Education Edition

    ERIC Educational Resources Information Center

    Johnson, L.; Adams Becker, S.; Estrada, V.; Freeman, A.

    2015-01-01

    The "NMC Horizon Report: 2015 Higher Education Edition" is a collaborative effort between the New Media Consortium (NMC) and the EDUCAUSE Learning Initiative (ELI). This 12th edition describes annual findings from the NMC Horizon Project, an ongoing research project designed to identify and describe emerging technologies likely to have…

  6. Seeing the Whole through Social Studies. Second Edition.

    ERIC Educational Resources Information Center

    Lindquist, Tarry

    Social studies currently has greater significance as teachers and students interact with content in a meaningful way. The second edition of this book describes a child-centered classroom where students can expose and explore critical content and connect their learning to the real world. This edition includes new features: "Teacher's Notebook"…

  7. Financing Education in a Climate of Change. Eighth Edition.

    ERIC Educational Resources Information Center

    Brimley, Vern, Jr.; Garfield, Rulon R.

    Since the publication of the seventh edition of this textbook in 1999, there have been many new developments in the education finance arena. Those changes are discussed in this eighth edition. Additional new material includes Internet resources, new exercises for further "laboratory" work, updated figures and tables, and fresh information on court…

  8. Coming on Center: Essays in English Education. Second Edition.

    ERIC Educational Resources Information Center

    Moffett, James

    This revised edition contains seven of James Moffett's talks and articles written since the original edition of "Coming on Center" was published in 1981, along with several of the earlier articles that are still timely. The book provides practical recommendations for specific ways of teaching reading and writing, shows why some conventional means…

  9. Introduction to Educational Administration: Standards, Theories, and Practice. Second Edition

    ERIC Educational Resources Information Center

    Fiore, Douglas J.

    2009-01-01

    Organized around the ISLLC standards, this text introduces students to the concepts and theories of educational leadership. The new edition adds coverage of such topics as data usage, ethics, innovative hiring practices, and student discipline. Appearing in the second edition are chapter-ending sections called "Point-Counterpoint" which prompt…

  10. Environmental Restoration and Waste Management: An Introduction. Student Edition.

    ERIC Educational Resources Information Center

    Department of Energy, Washington, DC.

    This technical document focuses on the Department of Energy's (DOE) efforts to restore the environment and manage nuclear waste. This student edition was rewritten and edited by a team of high school students in order to make it "user-friendly" for high school students and the general public. The document focuses on the efforts of the Office of…

  11. Quantifying On and Off-Target Genome Editing

    PubMed Central

    Hendel, Ayal; Fine, Eli J; Bao, Gang; Porteus, Matthew H

    2015-01-01

    Genome editing with engineered nucleases is a rapidly growing field, thanks to transformative technologies that allow researchers to precisely alter genomes for numerous applications, including basic research, biotechnology, and human gene therapy. While the capability to make precise and controlled changes at specified sites throughout the genome has grown tremendously in recent years, we still lack a comprehensive and standardized battery of assays for measuring the different genome editing outcomes created at endogenous genomic loci. Here, we review the existing assays for quantifying on and off-target genome editing and describe their utility in advancing the technology. We also highlight unmet assay needs for quantifying on and off-target genome editing outcomes and discuss their importance for the genome editing field. PMID:25595557

  12. Quantifying on- and off-target genome editing.

    PubMed

    Hendel, Ayal; Fine, Eli J; Bao, Gang; Porteus, Matthew H

    2015-02-01

    Genome editing with engineered nucleases is a rapidly growing field thanks to transformative technologies that allow researchers to precisely alter genomes for numerous applications including basic research, biotechnology, and human gene therapy. While the ability to make precise and controlled changes at specified sites throughout the genome has grown tremendously in recent years, we still lack a comprehensive and standardized battery of assays for measuring the different genome editing outcomes created at endogenous genomic loci. Here we review the existing assays for quantifying on- and off-target genome editing and describe their utility in advancing the technology. We also highlight unmet assay needs for quantifying on- and off-target genome editing outcomes and discuss their importance for the genome editing field. PMID:25595557

  13. Germline genome-editing research and its socioethical implications.

    PubMed

    Ishii, Tetsuya

    2015-08-01

    Genetically modifying eggs, sperm, and zygotes ('germline' modification) can impact on the entire body of the resulting individual and on subsequent generations. With the advent of genome-editing technology, human germline gene modification is no longer theoretical. Owing to increasing concerns about human germline gene modification, a voluntary moratorium on human genome-editing research and/or the clinical application of human germline genome editing has recently been called for. However, whether such research should be suspended or encouraged warrants careful consideration. The present article reviews recent research on mammalian germline genome editing, discusses the importance of public dialogue on the socioethical implications of human germline genome-editing research, and considers the relevant guidelines and legislation in different countries. PMID:26078206

  14. Divergent RNA editing frequencies in hornwort mitochondrial nad5 sequences.

    PubMed

    Duff, R Joel

    2006-02-01

    Hornwort mitochondrial genomes have some of the highest rates of RNA editing among plants. Comparison of eleven partial mitochondrial nad5 genomic and cDNA sequences from diverse taxa of hornworts reveal 125 edited sites in only 1107 nt. No single sample exhibits more than half of these sites. Ten of the 11 hornwort taxa have between 35 and 54 edited sties each; whereas, the eleventh taxon, Leiosporoceros, which represents a potential sister taxa to all other hornworts, has only eight sites. Comparison of multiple cDNA sequences from several individuals reveals the presence of many immature transcripts showing the heterogonous nature of the progression of editing. Phylogenetic analyses of hornwort genomic and cDNAs sequences reveal that 65 of the 94 phylogenetically informative sites within the hornwort clade are edited positions. PMID:16376027

  15. Genome editing: intellectual property and product development in plant biotechnology.

    PubMed

    Schinkel, Helga; Schillberg, Stefan

    2016-07-01

    Genome editing is a revolutionary technology in molecular biology. While scientists are fascinated with the unlimited possibilities provided by directed and controlled changes in DNA in eukaryotes and have eagerly adopted such tools for their own experiments, an understanding of the intellectual property (IP) implications involved in bringing genome editing-derived products to market is often lacking. Due to the ingenuity of genome editing, the time between new product conception and its actual existence can be relatively short; therefore knowledge about IP of the various genome editing methods is relevant. This point must be regarded in a national framework as patents are instituted nationally. Therefore, when designing scientific work that could lead to a product, it is worthwhile to consider the different methods used for genome editing not only for their scientific merits but also for their compatibility with a speedy and reliable launch into the desired market. PMID:27146974

  16. Profiling RNA editing in human tissues: towards the inosinome Atlas

    PubMed Central

    Picardi, Ernesto; Manzari, Caterina; Mastropasqua, Francesca; Aiello, Italia; D’Erchia, Anna Maria; Pesole, Graziano

    2015-01-01

    Adenine to Inosine RNA editing is a widespread co- and post-transcriptional mechanism mediated by ADAR enzymes acting on double stranded RNA. It has a plethora of biological effects, appears to be particularly pervasive in humans with respect to other mammals, and is implicated in a number of diverse human pathologies. Here we present the first human inosinome atlas comprising 3,041,422 A-to-I events identified in six tissues from three healthy individuals. Matched directional total-RNA-Seq and whole genome sequence datasets were generated and analysed within a dedicated computational framework, also capable of detecting hyper-edited reads. Inosinome profiles are tissue specific and edited gene sets consistently show enrichment of genes involved in neurological disorders and cancer. Overall frequency of editing also varies, but is strongly correlated with ADAR expression levels. The inosinome database is available at: http://srv00.ibbe.cnr.it/editing/. PMID:26449202

  17. Commercial and Industrial Wiring. Fourth Edition. Teacher Edition [and] Student Guide [and] Student Workbook 1 [and] Student Workbook 2.

    ERIC Educational Resources Information Center

    Kaltwasser, Stan; Flowers, Gary

    Commercial and Industrial Wiring, third in a series of three wiring publications, includes the additional technical knowledge and applications required for job entry in the commercial and industrial wiring trade. Instructional materials include a teacher edition, student guide, and two student workbooks. The teacher edition begins with…

  18. First Season Catfish Farming. A Workbook for Beginning Pond and Cage Culture of Channel Catfish. Teacher Edition and Student Edition.

    ERIC Educational Resources Information Center

    Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This workbook, comprised of both the teacher and student editions, presents guidelines useful for first-year catfish farmers in Oklahoma using pond or cage cultures to raise channel catfish. The teacher edition is a set of unit guidelines only. Contents include a list of suggested readings, important addresses with types of information available…

  19. Basic Wiring. Fourth Edition. Teacher Edition [and] Student Guide [and] Student Workbook 1 [and] Student Workbook 2.

    ERIC Educational Resources Information Center

    Kaltwasser, Stan; Flowers, Gary; Blasingame, Don

    Basic Wiring, first in a series of three wiring publications, serves as the foundation for students enrolled in a wiring program. It is a prerequisite to Commercial and Industrial Wiring or Residential Wiring. Instructional materials include a teacher edition, student guide, and two student workbooks. The teacher edition begins with introductory…

  20. High-Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells

    PubMed Central

    Carlson-Stevermer, Jared; Goedland, Madelyn; Steyer, Benjamin; Movaghar, Arezoo; Lou, Meng; Kohlenberg, Lucille; Prestil, Ryan; Saha, Krishanu

    2016-01-01

    Summary CRISPR-Cas9 gene editing of human cells and tissues holds much promise to advance medicine and biology, but standard editing methods require weeks to months of reagent preparation and selection where much or all of the initial edited samples are destroyed during analysis. ArrayEdit, a simple approach utilizing surface-modified multiwell plates containing one-pot transcribed single-guide RNAs, separates thousands of edited cell populations for automated, live, high-content imaging and analysis. The approach lowers the time and cost of gene editing and produces edited human embryonic stem cells at high efficiencies. Edited genes can be expressed in both pluripotent stem cells and differentiated cells. This preclinical platform adds important capabilities to observe editing and selection in situ within complex structures generated by human cells, ultimately enabling optical and other molecular perturbations in the editing workflow that could refine the specificity and versatility of gene editing. PMID:26771356

  1. Occurrence of plastid RNA editing in all major lineages of land plants

    PubMed Central

    Freyer, Regina; Kiefer-Meyer, Marie-Christine; Kössel, Hans

    1997-01-01

    RNA editing changes posttranscriptionally single nucleotides in chloroplast-encoded transcripts. Although much work has been done on mechanistic and functional aspects of plastid editing, little is known about evolutionary aspects of this RNA processing step. To gain a better understanding of the evolution of RNA editing in plastids, we have investigated the editing patterns in ndhB and rbcL transcripts from various species comprising all major groups of land plants. Our results indicate that RNA editing occurs in plastids of bryophytes, fern allies, true ferns, gymnosperms, and angiosperms. Both editing frequencies and editing patterns show a remarkable degree of interspecies variation. Furthermore, we have found that neither plastid editing frequencies nor the editing pattern of a specific transcript correlate with the phylogenetic tree of the plant kingdom. The poor evolutionary conservation of editing sites among closely related species as well as the occurrence of single species-specific editing sites suggest that the differences in the editing patterns and editing frequencies are probably due both to independent loss and to gain of editing sites. In addition, our results indicate that RNA editing is a relatively ancient process that probably predates the evolution of land plants. This supposition is in good agreement with the phylogenetic data obtained for plant mitochondrial RNA editing, thus providing additional evidence for common evolutionary roots of the two plant organellar editing systems. PMID:9177209

  2. Mine ventilation and air conditioning. 3. edition

    SciTech Connect

    Hartman, H.L.; Mutmansky, J.M.; Ramani, R.V.; Wang, Y.J.

    1998-12-31

    This revised edition presents an engineering design approach to ventilation and air conditioning as part of the comprehensive environmental control of the mine atmosphere. It provides an in-depth look, for practitioners who design and operate mines, into the health and safety aspects of environmental conditions in the underground workplace. The contents include: Environmental control of the mine atmosphere; Properties and behavior of air; Mine air-quality control; Mine gases; Dusts and other mine aerosols; Mine ventilation; Airflow through mine openings and ducts; Mine ventilation circuits and networks; Natural ventilation; Fan application to mines; Auxiliary ventilation and controlled recirculation; Economics of airflow; Control of mine fires and explosions; Mine air conditioning; Heat sources and effect in mines; Mine air conditioning systems; Appendices; References; Answers to selected problems; and Index.

  3. Stirling engine design manual. Second edition

    SciTech Connect

    Martini, W.R.

    1983-01-01

    This manual is intended to serve as an introduction to Stirling cycle heat engines, as a key to the available literature on Stirling engines and to identify non-proprietary Stirling engine design methodologies. Two different fully described Stirling engines are discussed. Engine design methods are categorized as first-order, second-order, and third-order with increased order number indicating increased complexity. FORTRAN programs are listed for both an isothermal second-order design program and an adiabatic second-order design program. Third-order methods are explained and enumerated. In this second edition of the Stirling Engine Design Manual the references have been updated. A revised personal and corporate author index is given and an expanded directory lists over 80 individuals and companies active in Stirling engines.

  4. Astronomical catalog desk reference, 1994 edition

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The Astronomical Catalog Desk Reference is designed to aid astronomers in locating machine readable catalogs in the Astronomical Data Center (ADC) archives. The key reference components of this document are as follows: A listing of shortened titles for all catalogs available from the ADC (includes the name of the lead author and year of publication), brief descriptions of over 300 astronomical catalogs, an index of ADC catalog numbers by subject keyword, and an index of ADC catalog numbers by author. The heart of this document is the set of brief descriptions generated by the ADC staff. The 1994 edition of the Astronomical Catalog Desk Reference contains descriptions for over one third of the catalogs in the ADC archives. Readers are encouraged to refer to this section for concise summaries of those catalogs and their contents.

  5. Stirling engine design manual, 2nd edition

    NASA Technical Reports Server (NTRS)

    Martini, W. R.

    1983-01-01

    This manual is intended to serve as an introduction to Stirling cycle heat engines, as a key to the available literature on Stirling engines and to identify nonproprietary Stirling engine design methodologies. Two different fully described Stirling engines are discussed. Engine design methods are categorized as first order, second order, and third order with increased order number indicating increased complexity. FORTRAN programs are listed for both an isothermal second order design program and an adiabatic second order design program. Third order methods are explained and enumerated. In this second edition of the manual the references are updated. A revised personal and corporate author index is given and an expanded directory lists over 80 individuals and companies active in Stirling engines.

  6. National solar energy education directory. Second edition

    SciTech Connect

    Corcoleotes, G; Cronin, S; Kramer, K; O'Connor, K

    1980-01-01

    The information contained in this directory is derived from responses to a national survey of educational institutions and organizations involved in solar energy educational activities beyond the secondary school level. Phone calls and follow-up mail requests were used to gather additional information when necessary. Every survey instrument was read, coded, and edited before entry into the data base from which this directory was produced. The Directory is organized alphabetically by state. Institutions and organizations within each state are categorized according to type (Colleges and Universities, Junior/Community Colleges, Vocational/Technical Schools, and Other Educational Institutions and Organizations) and listed alphabetically within these categories. Within each institutional listing the amount of information provided will vary according to the completeness of the survey response received from that institution. (MHR)

  7. TALE: a tale of genome editing.

    PubMed

    Zhang, Mingjie; Wang, Feng; Li, Shifei; Wang, Yan; Bai, Yun; Xu, Xueqing

    2014-01-01

    Transcription activator-like effectors (TALEs), first identified in Xanthomonas bacteria, are naturally occurring or artificially designed proteins that modulate gene transcription. These proteins recognize and bind DNA sequences based on a variable numbers of tandem repeats. Each repeat is comprised of a set of ∼ 34 conserved amino acids; within this conserved domain, there are usually two amino acids that distinguish one TALE from another. Interestingly, TALEs have revealed a simple cipher for the one-to-one recognition of proteins for DNA bases. Synthetic TALEs have been used to successfully target genes in a variety of species, including humans. Depending on the type of functional domain that is fused to the TALE of interest, these proteins can have diverse biological effects. For example, after binding DNA, TALEs fused to transcriptional activation domains can function as robust transcription factors (TALE-TFs), while fused to restriction endonucleases (TALENs) can cut DNA. Targeted genome editing, in theory, is capable of modifying any endogenous gene sequence of interest; this can be performed in cells or organisms, and may be applied to clinical gene-based therapies in the future. With current technologies, highly accurate, specific, and reliable gene editing cannot be achieved. Thus, recognition and binding mechanisms governing TALE biology are currently hot research areas. In this review, we summarize the major advances in TALE technology over the past several years with a focus on the interaction between TALEs and DNA, TALE design and construction, potential applications for this technology, and unique characteristics that make TALEs superior to zinc finger endonucleases. PMID:24291598

  8. Identification of candidate mitochondrial RNA editing ligases from Trypanosoma brucei.

    PubMed Central

    McManus, M T; Shimamura, M; Grams, J; Hajduk, S L

    2001-01-01

    Most mitochondrial genes of Trypanosoma brucei do not contain the necessary information to make translatable mRNAs. These transcripts must undergo RNA editing, a posttranscriptional process by which uridine residues are added and deleted from mitochondrial mRNAs. RNA editing is believed to be catalyzed by a ribonucleoprotein complex containing endonucleolytic, terminal uridylyl transferase (TUTase), 3' uridine-specific exonucleolytic (U-exo), and ligase activities. None of the catalytic enzymes for RNA editing have been identified. Here we describe the identification of two candidate RNA ligases (48 and 52 kDa) that are core catalytic components of the T. brucei ribonucleoprotein editing complex. Both enzymes share homology to the covalent nucleotidyl transferase superfamily and contain five key signature motifs, including the active site KXXG. In this report, we present data on the proposed 48 kDa RNA editing ligase. We have prepared polyclonal antibodies against recombinant 48 kDa ligase that specifically recognize the trypanosome enzyme. When expressed in trypanosomes as an epitope-tagged fusion protein, the recombinant ligase localizes to the mitochondrion, associates with RNA editing complexes, and adenylates with ATP. These findings provide strong support for the enzymatic cascade model for kinetoplastid RNA editing. PMID:11233974

  9. Explanatory Supplement to the Astronomical Almanac, Third Edition

    NASA Astrophysics Data System (ADS)

    Seidelmann, P. Kenneth; Urban, S. E.

    2010-01-01

    "The Explanatory Supplement to the Astronomical Almanac" (hereafter "The Explanatory Supplement") is a comprehensive reference book on the topic of positional astronomy, covering the theories and algorithms used to produce "The Astronomical Almanac" (AsA), an annual publication produced jointly by the Nautical Almanac Office of the US Naval Observatory (USNO) and Her Majesty's Nautical Almanac Office (HMNAO) of the UK Hydrographic Office. The first edition of The Explanatory Supplement appeared in 1961 and was reprinted with amendments during the 1970s. The second edition was printed in 1992 and reprinted until 2006. Since the second edition, several changes have taken place in positional astronomy regarding reference systems and internationally accepted models, data sets, and computational methods; these have been incorporated into the AsA. Additionally, the data presented in the AsA have been modified over the years, with new tables being added and some being discontinued. Given these changes, a new edition of The Explanatory Supplement is appropriate. The third edition has been in development for the last few years and will be available in 2010. The book is organized similarly to the second (1991) edition, with each chapter written by subject matter experts. Authors from USNO and HMNAO contributed to the majority of the book, but there are authors from Jet Propulsion Laboratory, Technical University of Dresden, National Geospatial-Intelligence Agency, University of Texas Austin, and University of Virginia. This paper will discuss this latest edition of the Explanatory Supplement.

  10. The clinical applications of genome editing in HIV.

    PubMed

    Wang, Cathy X; Cannon, Paula M

    2016-05-26

    HIV/AIDS has long been at the forefront of the development of gene- and cell-based therapies. Although conventional gene therapy approaches typically involve the addition of anti-HIV genes to cells using semirandomly integrating viral vectors, newer genome editing technologies based on engineered nucleases are now allowing more precise genetic manipulations. The possible outcomes of genome editing include gene disruption, which has been most notably applied to the CCR5 coreceptor gene, or the introduction of small mutations or larger whole gene cassette insertions at a targeted locus. Disruption of CCR5 using zinc finger nucleases was the first-in-human application of genome editing and remains the most clinically advanced platform, with 7 completed or ongoing clinical trials in T cells and hematopoietic stem/progenitor cells (HSPCs). Here we review the laboratory and clinical findings of CCR5 editing in T cells and HSPCs for HIV therapy and summarize other promising genome editing approaches for future clinical development. In particular, recent advances in the delivery of genome editing reagents and the demonstration of highly efficient homology-directed editing in both T cells and HSPCs are expected to spur the development of even more sophisticated applications of this technology for HIV therapy. PMID:27053530

  11. RCARE: RNA Sequence Comparison and Annotation for RNA Editing

    PubMed Central

    2015-01-01

    The post-transcriptional sequence modification of transcripts through RNA editing is an important mechanism for regulating protein function and is associated with human disease phenotypes. The identification of RNA editing or RNA-DNA difference (RDD) sites is a fundamental step in the study of RNA editing. However, a substantial number of false-positive RDD sites have been identified recently. A major challenge in identifying RDD sites is to distinguish between the true RNA editing sites and the false positives. Furthermore, determining the location of condition-specific RDD sites and elucidating their functional roles will help toward understanding various biological phenomena that are mediated by RNA editing. The present study developed RNA-sequence comparison and annotation for RNA editing (RCARE) for searching, annotating, and visualizing RDD sites using thousands of previously known editing sites, which can be used for comparative analyses between multiple samples. RCARE also provides evidence for improving the reliability of identified RDD sites. RCARE is a web-based comparison, annotation, and visualization tool, which provides rich biological annotations and useful summary plots. The developers of previous tools that identify or annotate RNA-editing sites seldom mention the reliability of their respective tools. In order to address the issue, RCARE utilizes a number of scientific publications and databases to find specific documentations respective to a particular RNA-editing site, which generates evidence levels to convey the reliability of RCARE. Sequence-based alignment files can be converted into VCF files using a Python script and uploaded to the RCARE server for further analysis. RCARE is available for free at http://www.snubi.org/software/rcare/. PMID:26043858

  12. User-friendly graph editing for procedural modeling of buildings.

    PubMed

    Patow, Gustavo

    2012-01-01

    A proposed rule-based editing metaphor intuitively lets artists create buildings without changing their workflow. It's based on the realization that the rule base represents a directed acyclic graph and on a shift in the development paradigm from product-based to rule-based representations. Users can visually add or edit rules, connect them to control the workflow, and easily create commands that expand the artist's toolbox (for example, Boolean operations or local controlling operators). This approach opens new possibilities, from model verification to model editing through graph rewriting. PMID:24804948

  13. Educational Measurement. Fourth Edition. ACE/Praeger Series on Higher Education

    ERIC Educational Resources Information Center

    Brennan, Robert L., Ed.

    2006-01-01

    "Educational Measurement" has been the bible in its field since the first edition was published by ACE in 1951. The importance of this fourth edition of "Educational Measurement" is to extensively update and extend the topics treated in the previous three editions. As such, the fourth edition documents progress in the field and…

  14. Review of the Fourth Edition of Soil Microbiology, Ecology, and Biochemistry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The 4th edition of Soil Microbiology, Ecology, and Biochemistry Edited by Eldor Paul continues in the vein of the 3rd edition by providing an excellent, broad-reaching introduction to soil biology. The new edition improves on the previous by providing extensive supplementary materials, links to outs...

  15. Educational Measurement. Fourth Edition. ACE/Praeger Series on Higher Education

    ERIC Educational Resources Information Center

    Brennan, Robert L., Ed.

    2006-01-01

    "Educational Measurement" has been the bible in its field since the first edition was published by ACE in 1951. The importance of this fourth edition of "Educational Measurement" is to extensively update and extend the topics treated in the previous three editions. As such, the fourth edition documents progress in the field and provides critical…

  16. Resources on Professional Development Schools: An Annotated Bibliography and Resource Guide. Third Edition.

    ERIC Educational Resources Information Center

    Abdal-Haqq, Ismat, Comp.

    This third edition includes 136 annotations and abstracts on Professional Development Schools (PDSs). Most resources are published since 1997. Because earlier editions of this publication are now out of print, annotations of several works from the second edition were chosen for inclusion in the current edition. In many cases, the older entries…

  17. Simultaneous Spectral Editing for γ-Aminobutyric Acid and Taurine Using Double Quantum Coherence Transfer

    NASA Astrophysics Data System (ADS)

    Lei, Hao; Peeling, James

    2000-03-01

    Conventional double quantum (DQ) editing techniques recover resonances of one metabolite at a time and are thus inefficient for monitoring metabolic changes involving several metabolites. A DQ coherence transfer double editing sequence using a dual-band DQ coherence read pulse is described here. The sequence permits simultaneous spectral editing for two metabolites with similar J coupling constants in a single scan. Simultaneous editing for taurine and γ-aminobutyric acid (GABA) is demonstrated using solution phantoms and rat brain tissue. Selectivity of the double editing sequence for the target metabolites is as good as that achieved using conventional DQ editing which selects each metabolite individually. With experimental parameters of the double editing sequence chosen to optimize GABA editing, the sensitivity for GABA detection is the same as that with GABA editing only, while the sensitivity for taurine detection is decreased slightly compared to that with taurine editing only.

  18. Air Sampling Instruments for Evaluation of Atmospheric Contaminants. Fourth Edition.

    ERIC Educational Resources Information Center

    American Conference of Governmental Industrial Hygienists, Cincinnati, OH.

    This text, a revision and extension of the first three editions, consists of papers discussing the basic considerations in sampling air for specific purposes, sampler calibration, systems components, sample collectors, and descriptions of air-sampling instruments. (BT)

  19. Advancing Crop Transformation in the Era of Genome Editing

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than thirty years of technological advances. Genome editing provides new opportunities to...

  20. Towards social acceptance of plant breeding by genome editing.

    PubMed

    Araki, Motoko; Ishii, Tetsuya

    2015-03-01

    Although genome-editing technologies facilitate efficient plant breeding without introducing a transgene, it is creating indistinct boundaries in the regulation of genetically modified organisms (GMOs). Rapid advances in plant breeding by genome-editing require the establishment of a new global policy for the new biotechnology, while filling the gap between process-based and product-based GMO regulations. In this Opinion article we review recent developments in producing major crops using genome-editing, and we propose a regulatory model that takes into account the various methodologies to achieve genetic modifications as well as the resulting types of mutation. Moreover, we discuss the future integration of genome-editing crops into society, specifically a possible response to the 'Right to Know' movement which demands labeling of food that contains genetically engineered ingredients. PMID:25726138

  1. The emerging role of RNA editing in plasticity

    PubMed Central

    Rosenthal, Joshua J. C.

    2015-01-01

    ABSTRACT All true metazoans modify their RNAs by converting specific adenosine residues to inosine. Because inosine binds to cytosine, it is a biological mimic for guanosine. This subtle change, termed RNA editing, can have diverse effects on various RNA-mediated cellular pathways, including RNA interference, innate immunity, retrotransposon defense and messenger RNA recoding. Because RNA editing can be regulated, it is an ideal tool for increasing genetic diversity, adaptation and environmental acclimation. This review will cover the following themes related to RNA editing: (1) how it is used to modify different cellular RNAs, (2) how frequently it is used by different organisms to recode mRNA, (3) how specific recoding events regulate protein function, (4) how it is used in adaptation and (5) emerging evidence that it can be used for acclimation. Organismal biologists with an interest in adaptation and acclimation, but with little knowledge of RNA editing, are the intended audience. PMID:26085659

  2. Application of binomial-edited CPMG to shale characterization

    NASA Astrophysics Data System (ADS)

    Washburn, Kathryn E.; Birdwell, Justin E.

    2014-09-01

    Unconventional shale resources may contain a significant amount of hydrogen in organic solids such as kerogen, but it is not possible to directly detect these solids with many NMR systems. Binomial-edited pulse sequences capitalize on magnetization transfer between solids, semi-solids, and liquids to provide an indirect method of detecting solid organic materials in shales. When the organic solids can be directly measured, binomial-editing helps distinguish between different phases. We applied a binomial-edited CPMG pulse sequence to a range of natural and experimentally-altered shale samples. The most substantial signal loss is seen in shales rich in organic solids while fluids associated with inorganic pores seem essentially unaffected. This suggests that binomial-editing is a potential method for determining fluid locations, solid organic content, and kerogen-bitumen discrimination.

  3. A-to-I RNA editing is induced upon hypoxia.

    PubMed

    Nevo-Caspi, Yael; Amariglio, Ninette; Rechavi, Gideon; Paret, Gideon

    2011-06-01

    Regulating gene expression is part of a cell's response to hypoxia. A-to-I RNA editing is an epigenetic phenomenon that can contribute to RNA and protein levels and to isoform diversity. In this study, we identified alterations in the levels of RNA editing following hypoxic stress in three genes: MED13, STAT3, and F11R. Changes in editing levels were associated with changes in RNA levels. These results suggest that A-to-I RNA editing may be one of the mechanisms used by cells to regulate changes in gene expression after hypoxia. These findings could lead to a novel therapeutic approach and better health care for children with hypoxemia. PMID:21330951

  4. Efficient parallel algorithms for string editing and related problems

    NASA Technical Reports Server (NTRS)

    Apostolico, Alberto; Atallah, Mikhail J.; Larmore, Lawrence; Mcfaddin, H. S.

    1988-01-01

    The string editing problem for input strings x and y consists of transforming x into y by performing a series of weighted edit operations on x of overall minimum cost. An edit operation on x can be the deletion of a symbol from x, the insertion of a symbol in x or the substitution of a symbol x with another symbol. This problem has a well known O((absolute value of x)(absolute value of y)) time sequential solution (25). The efficient Program Requirements Analysis Methods (PRAM) parallel algorithms for the string editing problem are given. If m = ((absolute value of x),(absolute value of y)) and n = max((absolute value of x),(absolute value of y)), then the CREW bound is O (log m log n) time with O (mn/log m) processors. In all algorithms, space is O (mn).

  5. Epigenome Editing: State of the Art, Concepts, and Perspectives.

    PubMed

    Kungulovski, Goran; Jeltsch, Albert

    2016-02-01

    Epigenome editing refers to the directed alteration of chromatin marks at specific genomic loci by using targeted EpiEffectors which comprise designed DNA recognition domains (zinc finger, TAL effector, or modified CRISPR/Cas9 complex) and catalytic domains from a chromatin-modifying enzyme. Epigenome editing is a promising approach for durable gene regulation, with many applications in basic research including the investigation of the regulatory functions and logic of chromatin modifications and cellular reprogramming. From a clinical point of view, targeted regulation of disease-related genes offers novel therapeutic avenues for many diseases. We review here the progress made in this field and discuss open questions in epigenetic regulation and its stability, methods to increase the specificity of epigenome editing, and improved delivery methods for targeted EpiEffectors. Future work will reveal if the approach of epigenome editing fulfills its great promise in basic research and clinical applications. PMID:26732754

  6. Model for Codon Position Bias in RNA Editing

    NASA Astrophysics Data System (ADS)

    Liu, Tsunglin; Bundschuh, Ralf

    2005-08-01

    RNA editing can be crucial for the expression of genetic information via inserting, deleting, or substituting a few nucleotides at specific positions in an RNA sequence. Within coding regions in an RNA sequence, editing usually occurs with a certain bias in choosing the positions of the editing sites. In the mitochondrial genes of Physarum polycephalum, many more editing events have been observed at the third codon position than at the first and second, while in some plant mitochondria the second codon position dominates. Here we propose an evolutionary model that explains this bias as the basis of selection at the protein level. The model predicts a distribution of the three positions rather close to the experimental observation in Physarum. This suggests that the codon position bias in Physarum is mainly a consequence of selection at the protein level.

  7. A model for codon position bias in RNA editing

    NASA Astrophysics Data System (ADS)

    Bundschuh, Ralf; Liu, Tsunglin

    2006-03-01

    RNA editing can be crucial for the expression of genetic information via inserting, deleting, or substituting a few nucleotides at specific positions in an RNA sequence. Within coding regions in an RNA sequence, editing usually occurs with a certain bias in choosing the positions of the editing sites. In the mitochondrial genes of Physarum polycephalum, many more editing events have been observed at the third codon position than at the first and second, while in some plant mitochondria the second codon position dominates. Here we propose an evolutionary model that explains this bias as the basis of selection at the protein level. The model predicts a distribution of the three positions rather close to the experimental observation in Physarum. This suggests that the codon position bias in Physarum is mainly a consequence of selection at the protein level.

  8. A Method for Genome Editing in Human Pluripotent Stem Cells.

    PubMed

    Smith, Cory; Ye, Zhaohui; Cheng, Linzhao

    2016-01-01

    Human pluripotent stem cells (PSCs) hold great potential for regenerative medicine and currently are being used as a research tool for basic discovery and disease modeling. To evaluate the role of a single genetic variant, a system of genome editing is needed to precisely mutate any desired DNA sequence in isolation and measure its effect on phenotype when compared to the isogenic parental PSC from which it was derived. This protocol describes the general targeting schemes used by researchers to edit PSCs to knock out, knock-in, or precisely alter a single nucleotide, covering conditions for electroporation, clonal isolation, and screening of edited PSCs for the targeted mutation. These recent advances simplify the procedure for genome editing, allowing individual researchers to induce nearly any desired mutation to further study its function or to reverse a disease-causing variant for future applications in regenerative medicine. PMID:27037073

  9. The universe, life, and intelligence (Sixth enlarged edition)

    NASA Astrophysics Data System (ADS)

    Shklovskii, Iosif Samuilovich

    This classic work examines the possibility of the existence of life (including intelligent life) on other planetary systems. This enlarged edition includes essays on the search for extraterrestrial civilizations and the possibility of communication with intelligent beings on other planets.

  10. Application of binomial-edited CPMG to shale characterization

    USGS Publications Warehouse

    Washburn, Kathryn E.; Birdwell, Justin E.

    2014-01-01

    Unconventional shale resources may contain a significant amount of hydrogen in organic solids such as kerogen, but it is not possible to directly detect these solids with many NMR systems. Binomial-edited pulse sequences capitalize on magnetization transfer between solids, semi-solids, and liquids to provide an indirect method of detecting solid organic materials in shales. When the organic solids can be directly measured, binomial-editing helps distinguish between different phases. We applied a binomial-edited CPMG pulse sequence to a range of natural and experimentally-altered shale samples. The most substantial signal loss is seen in shales rich in organic solids while fluids associated with inorganic pores seem essentially unaffected. This suggests that binomial-editing is a potential method for determining fluid locations, solid organic content, and kerogen–bitumen discrimination.

  11. Tethers in space handbook, second edition

    NASA Technical Reports Server (NTRS)

    Penzo, Paul A. (Editor); Ammann, Paul W. (Editor)

    1989-01-01

    The Tethers in Space Handbook, Second Edition represents an update to the initial volume issued in September 1986. As originally intended, this handbook is designed to serve as a reference manual for policy makers, program managers, educators, engineers, and scientists alike. It contains information for the uninitiated, providing insight into the fundamental behavior of tethers in space. For those familiar with space tethers, it includes a summary of past and ongoing studies and programs, a complete bibliography of tether publications, and names, addresses, and phone numbers of workers in the field. Perhaps its most valuable asset is the brief description of nearly 50 tether applications which have been proposed and analyzed over the past 10 years. The great variety of these applications, from energy generation to boosting satellites to gravity wave detection is an indication that tethers will play a significant part in the future of space development. This edition of the handbook preserves the major characteristics of the original; however, some significant rearrangements and additions have been made. The first section on Tether Programs has been brought up to date, and now includes a description of TSS-2, the aerodynamic NASA/Italian Space Agency (ASI) mission. Tether Applications follows, and this section has been substantially rearranged. First, the index and cross-reference for the applications have been simplified. Also, the categories have changed slightly, with Technology and Test changed to Aerodynamics, and the Constellations category removed. In reality, tether constellations may be applicable to many of the other categories, since it is simply a different way of using tethers. Finally, to separate out those applications which are obviously in the future, a Concepts category has been added. A new section included here on Conference Summaries recognizes the fact that the tether community is growing internationally, and that meetings provide a means of

  12. Uncovering RNA Editing Sites in Long Non-Coding RNAs

    PubMed Central

    Picardi, Ernesto; D’Erchia, Anna Maria; Gallo, Angela; Montalvo, Antonio; Pesole, Graziano

    2014-01-01

    RNA editing is an important co/post-transcriptional molecular process able to modify RNAs by nucleotide insertions/deletions or substitutions. In human, the most common RNA editing event involves the deamination of adenosine (A) into inosine (I) through the adenosine deaminase acting on RNA proteins. Although A-to-I editing can occur in both coding and non-coding RNAs, recent findings, based on RNA-seq experiments, have clearly demonstrated that a large fraction of RNA editing events alter non-coding RNAs sequences including untranslated regions of mRNAs, introns, long non-coding RNAs (lncRNAs), and low molecular weight RNAs (tRNA, miRNAs, and others). An accurate detection of A-to-I events occurring in non-coding RNAs is of utmost importance to clarify yet unknown functional roles of RNA editing in the context of gene expression regulation and maintenance of cell homeostasis. In the last few years, massive transcriptome sequencing has been employed to identify putative RNA editing changes at genome scale. Despite several efforts, the computational prediction of A-to-I sites in complete eukaryotic genomes is yet a challenging task. We have recently developed a software package, called REDItools, in order to simplify the detection of RNA editing events from deep sequencing data. In the present work, we show the potential of our tools in recovering A-to-I candidates from RNA-Seq experiments as well as guidelines to improve the RNA editing detection in non-coding RNAs, with specific attention to the lncRNAs. PMID:25538940

  13. [The Washington summit: orange light for genome editing?].

    PubMed

    Jordan, Bertrand

    2016-02-01

    The summit organised in early December 2015 considered in depth the various issues (technical, scientific, societal and ethical) raised by the prospect of genome editing using the extremely effective CRISPR system. Germline editing (for therapeutic or "enhancement" purposes) was stated to be irresponsible under current conditions, but the possibility that this could be considered in the future was not excluded; a mechanism for monitoring progress and possibly revising recommendations was proposed. PMID:26936181

  14. 3D Human Motion Editing and Synthesis: A Survey

    PubMed Central

    Wang, Xin; Chen, Qiudi; Wang, Wanliang

    2014-01-01

    The ways to compute the kinematics and dynamic quantities of human bodies in motion have been studied in many biomedical papers. This paper presents a comprehensive survey of 3D human motion editing and synthesis techniques. Firstly, four types of methods for 3D human motion synthesis are introduced and compared. Secondly, motion capture data representation, motion editing, and motion synthesis are reviewed successively. Finally, future research directions are suggested. PMID:25045395

  15. Molecular Biology of the Cell, Sixth Edition; ISBN: 9780815344643; and Molecular Biology of the Cell, Sixth Edition, The Problems Book; ISBN 9780815344537

    PubMed Central

    Bustin, Stephen

    2015-01-01

    The latest edition maintains the excellence and appeal of the previous editions. Its clear text and outstanding illustrations, together with the complementary problems book make this an essential companion for students and lecturers alike.

  16. Pervasive RNA editing among hornwort rbcL transcripts except Leiosporoceros.

    PubMed

    Duff, R Joel; Moore, Francisco B-G

    2005-11-01

    RNA editing affecting chloroplast and mitochondrial genomes has been identified in all major clades of land plants. The frequency of edited sites varies greatly between lineages but hornworts represent an extreme in propensity for editing in both their chloroplast and mitochondrial genomes. cDNA sequences from seven taxonomically diverse hornwort rbcL sequences combined with a survey of 13 additional DNA sequences for potential edited sites demonstrate the presence of 62 edited sites and predict a minimum of 10 additional sites. These 72 total edited sites represent 43 C-to-U and 28 U-to-C nucleotide conversions, with 1 site exhibiting editing in both directions. With one exception, all taxa are heavily edited, with each having from 20 to 34 edited sites. However, a single sample, Leiosporoceros, is shown to lack edited sites. Phylogenetic reconstruction of hornworts results in ambiguous resolution of Leiosporoceros depending on whether edited sites are maintained or eliminated from the analyses. Depending on the inferred relationship of Leiosporoceros to the hornworts, at least two explanations for the origin and maintenance of pervasive editing in hornworts are possible. The absence of edited sites in Leiosporoceros could represent either the absence or a low level of editing ability in the common ancestor of hornworts, as represented by Leiosporoceros, or the loss of editing sites in this lineage after the primary diversification events in the group. PMID:16177870

  17. Fundamentals of Physics, 6th Edition Enhanced Problems Version

    NASA Astrophysics Data System (ADS)

    Halliday, David; Resnick, Robert; Walker, Jearl

    2002-04-01

    No other text on the market today can match the success of Halliday, Resnick and Walker's Fundamentals of Physics. This text continues to outperform the competition year after year, and the new edition will be no exception. Intended for Calculus-based Physics courses, the 6th edition of this extraordinary text is a major redesign of the best-selling 5th edition, which still maintains many of the elements that led to its enormous success. Jearl Walker adds his unique style to this edition with the addition of new problems designed to capture, and keep, students' attention. Nearly all changes are based on suggestions from instructors and students using the 5th edition, from reviewer comments, and from research done on the process of learning. The primary goal of this text is to provide students with a solid understanding of fundamental physics concepts, and to help them apply this conceptual understanding to quantitative problem solving. The principal goal of Halliday-Resnick-Walker is to provide instructors with a tool by which they can teach students how to effectively read scientific material and successfully reason through scientific questions. To sharpen this tool, the Enhanced Problems Version of the sixth edition of Fundamentals of Physics contains over 1000 new, high-quality problems that require thought and reasoning rather than simplistic plugging of data into formulas.

  18. Genome editing in pluripotent stem cells: research and therapeutic applications.

    PubMed

    Deleidi, Michela; Yu, Cong

    2016-05-01

    Recent progress in human pluripotent stem cell (hPSC) and genome editing technologies has opened up new avenues for the investigation of human biology in health and disease as well as the development of therapeutic applications. Gene editing approaches with programmable nucleases have been successfully established in hPSCs and applied to study gene function, develop novel animal models and perform genetic and chemical screens. Several studies now show the successful editing of disease-linked alleles in somatic and patient-derived induced pluripotent stem cells (iPSCs) as well as in animal models. Importantly, initial clinical trials have shown the safety of programmable nucleases for ex vivo somatic gene therapy. In this context, the unlimited proliferation potential and the pluripotent properties of iPSCs may offer advantages for gene targeting approaches. However, many technical and safety issues still need to be addressed before genome-edited iPSCs are translated into the clinical setting. Here, we provide an overview of the available genome editing systems and discuss opportunities and perspectives for their application in basic research and clinical practice, with a particular focus on hPSC based research and gene therapy approaches. Finally, we discuss recent research on human germline genome editing and its social and ethical implications. PMID:26930470

  19. Statistical optimisation techniques in fatigue signal editing problem

    NASA Astrophysics Data System (ADS)

    Nopiah, Z. M.; Osman, M. H.; Baharin, N.; Abdullah, S.

    2015-02-01

    Success in fatigue signal editing is determined by the level of length reduction without compromising statistical constraints. A great reduction rate can be achieved by removing small amplitude cycles from the recorded signal. The long recorded signal sometimes renders the cycle-to-cycle editing process daunting. This has encouraged researchers to focus on the segment-based approach. This paper discusses joint application of the Running Damage Extraction (RDE) technique and single constrained Genetic Algorithm (GA) in fatigue signal editing optimisation.. In the first section, the RDE technique is used to restructure and summarise the fatigue strain. This technique combines the overlapping window and fatigue strain-life models. It is designed to identify and isolate the fatigue events that exist in the variable amplitude strain data into different segments whereby the retention of statistical parameters and the vibration energy are considered. In the second section, the fatigue data editing problem is formulated as a constrained single optimisation problem that can be solved using GA method. The GA produces the shortest edited fatigue signal by selecting appropriate segments from a pool of labelling segments. Challenges arise due to constraints on the segment selection by deviation level over three signal properties, namely cumulative fatigue damage, root mean square and kurtosis values. Experimental results over several case studies show that the idea of solving fatigue signal editing within a framework of optimisation is effective and automatic, and that the GA is robust for constrained segment selection.

  20. Statistical optimisation techniques in fatigue signal editing problem

    SciTech Connect

    Nopiah, Z. M.; Osman, M. H.; Baharin, N.; Abdullah, S.

    2015-02-03

    Success in fatigue signal editing is determined by the level of length reduction without compromising statistical constraints. A great reduction rate can be achieved by removing small amplitude cycles from the recorded signal. The long recorded signal sometimes renders the cycle-to-cycle editing process daunting. This has encouraged researchers to focus on the segment-based approach. This paper discusses joint application of the Running Damage Extraction (RDE) technique and single constrained Genetic Algorithm (GA) in fatigue signal editing optimisation.. In the first section, the RDE technique is used to restructure and summarise the fatigue strain. This technique combines the overlapping window and fatigue strain-life models. It is designed to identify and isolate the fatigue events that exist in the variable amplitude strain data into different segments whereby the retention of statistical parameters and the vibration energy are considered. In the second section, the fatigue data editing problem is formulated as a constrained single optimisation problem that can be solved using GA method. The GA produces the shortest edited fatigue signal by selecting appropriate segments from a pool of labelling segments. Challenges arise due to constraints on the segment selection by deviation level over three signal properties, namely cumulative fatigue damage, root mean square and kurtosis values. Experimental results over several case studies show that the idea of solving fatigue signal editing within a framework of optimisation is effective and automatic, and that the GA is robust for constrained segment selection.