Regulation of mRNA decay is an important step modulating gene expression. The stability of numerous eukaryotic mRNAs is controlled by adenosine/uridine-rich elements (AREs) located in their 3?UTR. In Saccharomyces cerevisiae, the Cth2 protein stimulates the decay of target ARE mRNAs on iron starvation. Cth2, and its mammalian homologue tristetraprolin, contains a characteristic tandem CCCH ...

. Effects of tactile interference stimulation on somatosensory evoked magnetic fields following tibial nerve1 Title: Transient and steady-state responses to mechanical stimulation of different fingers reveal finger stimulation evokes transient ERP responses that are smaller than the linear summation of ERP

Finger millet is an allotetraploid (2n = 4x = 36) grass that belongs to the Chloridoideae subfamily. A comparative analysis has been carried out to determine the relationship of the finger millet genome with that of rice. Six of the nine finger millet homoeologous groups corresponded to a single rice chromosome each. Each of the ...

Angioleiomyomas in the finger are rare and their preoperative diagnosis is difficult. Most of them are not associated with arteries and their chief complaint does not reveal pulsation tumor. We report a case of the angioleiomyoma in the finger which was misdiagnosed as arteriovenous fistula of tumor, based on the presence of pulsating ...

The creation of the artificial RING finger as ubiquitin-ligating enzyme (E3) has been demonstrated. In this study, by the alpha-helical region substitution between the EL5 RING finger and the Williams-Beuren syndrome transcription factor (WSTF) PHD finger, the artificial E3 (WSTF PHD_RING finger) was newly created. ...

"The Earth behaved much like putting your finger into a sponge ball and watching it slowly bounce back," said Christopher Cox, a research scientist ...

... finger printing using microsatellite markers revealed ... human neurofibromatosis (NF) tumor xenografts in ... bones and thus produce skeletal instability. ...

Helix 38 (H38) of the large ribosomal subunit, with a length of 110 �, reaches the small subunit through intersubunit bridge B1a. Previous cryo-EM studies revealed that the tip of H38 moves by more than 10 � from the non-ratcheted to the ratcheted state of the ribosome while mutational studies implicated a key role of flexible H38 in attenuation of translocation and in ...

Examination of the enzyme structure for acetylcholinesterase (AChE) reveals two sites of ligand interaction: The peripheral site (P-site) located at the entrance of the gorge, and the acylation site (A-site) at the base of the gorge. Our goal is to develo...

Examination of the enzyme structure for acetylcholinesterase (AChE) reveals two sites of ligand interaction: The peripheral site (P-site) located at the entrance of the gorge, and the acylation site (A-site) at the base of the gorge. Our goal is to develo...

Examination of the enzyme structure for acetylcholinesterase (AChE) reveals two sites of ligand interaction: The peripheral site (P-site) located at the entrance of the gorge, and the acylation site (A-site) at the base of the gorge. Our goal is to develo...

The degloving injuries of the digits and palm remain a persistent challenge. We used an antero- lateral thigh flap to treat an 18-year-old, right-handed male worker with degloving injuries of the index, middle and ring fingers. The flap was designated to wrap the entire circumference of three fingers sustaining degloving injury and to form mitten-hand. The ...

To improve the DNA hydrolytic activity of the zinc finger nuclease, we have created a new artificial zinc finger nuclease (ZWH4) by connecting two distinct zinc finger domains possessing different types of Zn(II) binding sites (Cys(2)His(2)- and His(4)-types). The overall fold of ZWH4 is similar to that of the wild-type Sp1 zinc ...

We study the stretch flow of a thin layer of Newtonian liquid constrained between two circular plates. The evolution of the interface of the originally circular bubble is studied when lifting one of the plates at a constant velocity and the observed pattern is related to the measured lifting force. By comparing experimental results to numerical simulations using a Darcy's law model we can account ...

We study the stretch flow of a thin layer of Newtonian liquid constrained between two circular plates. The evolution of the interface of the originally circular bubble is studied when lifting one of the plates at a constant velocity and the observed pattern is related to the measured lifting force. By comparing experimental results to numerical simulations using a Darcy's law model we can account ...

The purpose of this study was to evaluate the results of excision of the ulnar slip of the flexor digitorum superficialis tendon, with or without A1 pulley release, for the treatment of trigger finger in diabetic patients. We performed a retrospective review with long-term follow-up examinations. Short-term data was obtained on 18 consecutive patients (37 ...

Histone modifications are fundamental to chromatin structure and transcriptional regulation, and are recognized by a limited number of protein folds. Among these folds are PHD fingers, which are present in most chromatin modification complexes. To date, about 15 PHD finger domains have been structurally characterized, whereas hundreds of different ...

Zinc finger domains are structures that mediate sequence recognition for a large number of DNA-binding proteins. These domains consist of sequences of amino acids containing cysteine and histidine residues tetrahedrally coordinated to a zinc ion. In this report, we present a means to selectively inhibit a zinc finger transcription factor with cobalt(III) ...

Zinc finger domains are structures that mediate sequence recognition for a large number of DNA-binding proteins. These domains consist of sequences of amino acids containing cysteine and histidine residues tetrahedrally coordinated to a zinc ion. In this report, we present a means to selectively inhibit a zinc finger transcription factor with cobalt(III) ...

Timing plays an important role in perceiving and performing music. Finger tapping has been successfully used for analyzing timing processes (Fraisse, 1966, Fran?k et al., 1987, 1988). The aim of this study is to determine differences between musically trained and untrained subjects in their ability to follow repetitive rhythmic tonal patterns by finger ...

We report the case of a 23-year-old male presenting with carpal tunnel syndrome and a swelling over the flexor surface of the wrist. MRI findings were initially suggestive of a median nerve schwannoma but sonography (US) showed a heterogenous mass infiltrating the flexor tendons of the fingers and displacing the median nerve in the carpal tunnel. US findings were confirmed by ...

Classical C2H2 zinc finger proteins are amongst the most abundant transcription factors found in eukaryotes and the mechanisms through which they recognize their target genes have been extensively investigated. In general, a tandem array of three fingers separated by characteristic TGERP links is required for sequence-specific DNA recognition. ...

Handwriting impairments in Parkinson's disease (PD) have been associated with micrographia, i.e. diminished letter size. However, dyscoordination of the wrist and fingers may also contribute to handwriting deterioration in PD. To investigate this hypothesis, right-handed PD patients and controls were tested in performance of three types of cyclic wrist and ...

Handwriting impairments in Parkinson�s disease (PD) have been associated with micrographia, i.e. diminished letter size. However, dyscoordination of the wrist and fingers may also contribute to handwriting deterioration in PD. To investigate this hypothesis, right-handed PD patients and controls were tested in performance of three types of cyclic wrist and ...

Role of the arginine finger in Ras�RasGAP revealed by QM/MM calculations Henrik te Heesen, Klaus triphosphate is strongly accelerated GAP as compared to Ras alone. This is largely attributed to the arginine. The arginine finger is shown to act by displacing water out of the binding niche. The resulting enhanced

This study investigated whether neuromotor differences between adults who stutter (n=10) and gender- and age-matched non-stuttering adults (n=10) are not limited to the movements involved in speech production. Results revealed significant differences between groups on measures of lip and jaw closing (but not opening) movements during speech and in finger ...

It is often assumed that large diameter nerve fibres are affected selectively in patients with compressive peripheral nerve lesions. However, in five of 13 patients with the carpal tunnel syndrome, plethysmography revealed that the normal digital vasoconstrictor response to inspiration was abolished in the index finger but preserved in the little ...

It is well known that timing of rhythm production is disrupted by delayed auditory feedback (DAF), and that disruption varies with delay length. We tested the hypothesis that disruption depends on the state of the movement trajectory at the onset of DAF. Participants tapped isochronous rhythms at a rate specified by a metronome while hearing DAF (for piano tones) of differing lengths. Motion ...

Low-stringency hybridization of the Drosophila serendipity (sry) finger-coding sequences revealed copies of homologous DNA sequences in the genomes of members of the family Drosophilidae and higher vertebrates. sry h-1, a new Drosophila finger protein-coding gene isolated on the basis of this homology, encodes a 3.2-kilobase (kb) mRNA ...

Trigger fingers can be treated by open or percutaneous division of the A1 pulley. The open approach allows for visualization of the pulley, the tendon, and the adjacent neurovascular bundles. The percutaneous trigger finger release (PTFR) lacks an incision and is thought to lead to a quicker recovery, but the safety and efficacy of this blind procedure are ...

Trigger fingers can be treated by open or percutaneous division of the A1 pulley. The open approach allows for visualization of the pulley, the tendon, and the adjacent neurovascular bundles. The percutaneous trigger finger release (PTFR) lacks an incision and is thought to lead to a quicker recovery, but the safety and efficacy of this blind procedure are ...

Krueppel-like factor 4 (Klf4) belongs to the Sp/Klf family of zinc-finger transcription factors and is indispensable for terminal maturation of epithelial tissues. Furthermore, it is part of a small set of proteins that are used to generate pluripotent embryonic stem cells from differentiated tissues. Herein, we describe that a Klf4 zinc-finger domain ...

Recordings of the dominant finger during the reading of braille sentences by experienced readers reveal that the velocity of the finger changes frequently during the traverse of a line of text. These changes, not previously reported, involve a multitude of accelerations and decelerations, as well as reversals of direction. We ...

Fine-scale functional organization of the finger areas in the human primary somatosensory cortex was investigated by high-resolution BOLD MRI at 3 T using a multi-echo FLASH sequence with a voxel size of 2 mm(3). In six subjects independent tactile stimulation of the distal phalanx of the fingers of the right hand resulted in small circumscribed and barely ...

A matrix of miniature and flexible pressure sensors is proposed to measure the grip pressure distribution (GPD) at the hand-handle interface of a vibrating handle. The GPD was acquired under static and dynamic loads for various levels of grip forces and magnitudes of vibration at different discrete frequencies in the 20-1000 Hz range. The EMG of finger flexor muscles was ...

These experiments were designed to determine whether cutaneous input from a digit provides a general facilitation of the detection of movements applied to an adjacent digit. The ability to detect passive movements at the proximal interphalangeal joint of the right index finger was measured when cutaneous (and joint) input was removed (using local anaesthesia) from the tip of ...

The minimal DNA-binding domain of the yeast ADR1 transcription factor consists of two Cys2-His2 zinc fingers and an additional 20 residues N-terminal and proximal to the fingers. The accessory sequence likely plays a role in contacting DNA. Paramagnetic cobalt was incorporated into the fingers of an ADR1 DNA-binding construct (ADR1z) ...

Arsenic inhibits DNA repair and enhances the genotoxicity of DNA-damaging agents such as benzo[a]pyrene and ultraviolet radiation. Arsenic interaction with DNA repair proteins containing functional zinc finger motifs is one proposed mechanism to account for these observations. Here, we report that arsenite binds to both CCHC DNA-binding zinc fingers of the ...

Reports on an informal study that revealed that a significant number of people who hold a pencil with two fingers and a thumb have mixed cerebral dominance, that more females than males have mixed dominance, and that those with mixed dominance tend to be more imaginative. (FL)

Bluish skin discoloration Fingers that change color upon pressure Flank pain Swollen glands Exams and Tests A physical examination may reveal a swollen spleen, liver, and lymph...

Analysis of 43 cutaneous myxoid cysts in 40 patients with lesions of the fingers and toes revealed the most common clinical appearance to be a soft or fluctuant cystic nodule. The pathogenesis consists of fibroblastic proliferation in the corium, an incre...

Activated sludge flocs suspended in wet mounts on microscope slides were observed to sprout, finger-like, bacterial zoogloeae as a consequence of the outgrowth of bacteria from flocs. Photomicrographic and fluorescent antibody studies revealed that the ba...

Alternative polyadenylation (APA) has been shown to play an important role in gene expression regulation in animals and plants. However, the extent of sense and antisense APA at the genome level is not known. We developed a deep-sequencing protocol that queries the junctions of 3'UTR and poly(A) tails and confidently maps the poly(A) tags to the annotated genome. The results of this mapping show ...

Alternative polyadenylation (APA) has been shown to play an important role in gene expression regulation in animals and plants. However, the extent of sense and antisense APA at the genome level is not known. We developed a deep-sequencing protocol that queries the junctions of 3?UTR and poly(A) tails and confidently maps the poly(A) tags to the annotated genome. The results of this mapping show ...

The accurate identification of potential poly(A) sites has contributed to all many studies with regard to alternative polyadenylation. The aim of this study was the development of a machine-learning methodology that will help to discriminate real polyadenylation signals from randomly occurring signals in genomic sequence. Since previous studies have ...

A long-standing goal of molecular biologists has been to construct DNA-binding proteins for the control of gene expression. The classical Cys2His2 (C2H2) zinc finger design is ideally suited for such purposes. Discriminating between closely related DNA sequences both in vitro and in vivo, this naturally occurring design was adopted for engineering zinc ...

This report discusses the development and testing of prototype gloves for underground mining work. An analysis of HSAC hand-finger injuries revealed three high-risk jobs: laborer, mechanic, and roof bolter. Laboratory tests of dexterity and protection were performed using six off-the-shelf gloves. Two prototype gloves were developed. Both were ...

The present study investigates intrinsic preferences and tendencies in coordination of the wrist and finger movements during handwriting-like tasks. Movement of the inkless pen tip in nine right-handed subjects was registered with a digitizer. One circle-drawing task and four line-drawing tasks were included in the experiment. The line-drawing task included: (1) drawing with ...

1. Cutaneous reflexes have been recorded from the first dorsal interosseous muscle following electrical stimulation of the digital nerves of the index finger in man. Recordings have been obtained from 127 children, aged from 3 to 18 years while each performed an isometric abduction of the index finger and whole hand grips. 2. In the adult, reflexes ...

Dexterous use of the hand represents a sophisticated sensorimotor function. In behaviors such as playing the piano, it can involve strong temporal and spatial constraints. The purpose of this study was to determine fundamental patterns of covariation of motion across joints and digits of the human hand. Joint motion was recorded while five expert pianists played thirty excerpts from musical ...

Understanding the transformation of tendon forces into joint torques would greatly aid in the investigation of the complex temporal and spatial coordination of multiple muscles in finger movements. In this study, the effects of the finger posture on the tendon force transmission within the finger extensor apparatus were investigated. ...

The ability to modulate bilateral finger tapping in time to different frequencies of an auditory beat was studied. Twenty children, 7 years of age, 10 with and 10 without developmental coordination disorder (DCD), and 10 adults tapped their left index and right middle fingers in an alternating pattern in time with an auditory signal for 15s (four trials ...

The crystal structure of the snake long ?-neurotoxin, ?-cobratoxin, bound to the pentameric acetylcholine-binding protein (AChBP) from Lymnaea stagnalis, was solved from good quality density maps despite a 4.2 � overall resolution. The structure unambiguously reveals the positions and orientations of all five three-fingered toxin molecules inserted at ...

Previously, we have identified a novel CCCH zinc finger protein family as negative regulators of macrophage activation. To gain an overall insight into the entire CCCH zinc finger gene family and to evaluate their potential role in macrophage activation, here we performed a genome-wide survey of CCCH zinc finger genes in mouse and ...

RecQ family helicases are essential in maintaining chromosomal DNA stability and integrity. Despite extensive studies, the mechanisms of these enzymes are still poorly understood. Crystal structures of many helicases reveal a highly conserved arginine residue located near the ?-phosphate of ATP. This residue is widely recognized as an arginine finger, and ...

SUMMARYHuman autoimmune regulator (AIRE) functions to control thymic expression of tissue-specific antigens via sequence-specific histone H3 recognition by its plant homeodomain (PHD) finger. Mutations in the AIRE PHD finger have been linked to autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). Here we report the three-dimensional ...

Rehabilitation therapists and orthopedists regard degenerative changes (DCs) of the articular cartilage or osteoarthritis in the finger joint as the major factors that interfere with normal grasp and pinch. However, previous research has concentrated on DCs in the carpometacarpal joint (CM) of the thumb. The present study revealed some general tendencies ...

With all the technology today, the authors were surprised to read a recent British study that found a connection between the length of a woman's index (2D) and ring (4D) fingers to her athletic ability. Upon further investigation they found that many studies have examined the relationship between the length of the index finger (2D) to the ring ...

The development of immiscible viscous fingering patterns in a rotating Hele-Shaw cell is investigated. We focus on understanding how the time evolution and the resulting morphologies are affected by the action of the Coriolis force. The problem is approached analytically and numerically by employing a vortex sheet formalism. The vortex sheet strength and a linear dispersion ...

Bacteriorhodopsin, the photosynthetic protein of Halobacterium salinarum, is optimally expressed under anaerobic growth conditions. We identified Brz (OE3104F, bacteriorhodopsin-regulating zinc finger protein), a new regulator of the bop gene. It is a small protein with a zinc finger motif, encoded directly upstream of the bop gene in the same orientation. ...

We investigated the central programming of overarm throws by determining whether throws to spatially separate targets in the vertical direction (sagittal plane) are produced by changes in hand (i.e., finger) path direction or by changes in the timing of ball release. Six skilled throwers made 30 throws at the same speed with a baseball, from a sitting position with the chest ...

The transition from early to late stage infection by adenovirus involves a change in mRNA expression from the adenovirus major late transcription unit (AdMLTU). This early to late switch centers around alternative selection of one of five poly (A) sites (L1-L5) that code for the major structural proteins of Adenovirus. During the early stage of infection, ...

Analysis of the amino acid sequence encoded by the familial breast and ovarian cancer susceptibility gene, BRCA1 [Miki et al. (1994) Science 266, 66-71], revealed the presence of an amino-terminal RING finger domain, a zinc binding motif found in a variety of proteins. Previously determined structures of two RING finger peptides from ...

DNA-binding functionality among transcription factor proteins is afforded by a number of structural motifs, such as the helix-turn-helix, helix-loop-helix, and zinc finger domains. The common thread among these diverse structures is their sequence-specific binding to essential promoter or other genetic regulatory sequences with high selectivity and affinity. One such motif, ...

The zinc finger HIT domain is a sequence motif found in many proteins, including thyroid hormone receptor interacting protein 3 (TRIP-3), which is possibly involved in maturity-onset diabetes of the young (MODY). Novel zinc finger motifs are suggested to play important roles in gene regulation and chromatin remodeling. Here, we determined the ...

Estrogen receptor (ER, alpha isoform) is a 67 kDa zinc finger transcription factor that plays a fundamental role in both normal reproductive gland development and breast carcinogenesis, and also represents a critical molecular target for breast cancer therapy. We are investigating the structural consequences of chemical exposures thought to modify essential zinc ...

Ribosomal protein L11 (RPL11) has been shown to activate p53 by binding to MDM2 and negating its p53-supression activity in response to ribosomal stress. Although a mutation at Cys 305 within the zinc finger domain of MDM2 has been shown to drastically impair MDM2 interaction with RPL11 and thus escapes the inhibition by this ribosomal protein, it still remains elusive whether ...

Certain DNA-binding proteins that regulate gene expression contain single or multiple copies of short polypeptide sequences, approximately 30 residues long, consisting of combinations of four Cys or His residues at defined spacing, so that Zn++ is complexed in tetrahedral coordination with the respective thiol-sulfur and/or imidazole-nitrogen atoms. The Zn++ ion evidently serves as a strut that ...

The nuclear pore complex (NPC) resides in circular openings within the nuclear envelope and serves as the sole conduit to facilitate nucleo-cytoplasmic transport in eukaryotes. The asymmetric distribution of the small G protein Ran across the nuclear envelope regulates directionality of protein transport. Ran interacts with the NPC of metazoa via two asymmetrically localized components, Nup153 at ...

The nuclear pore complex (NPC) resides in circular openings within the nuclear envelope and serves as the sole conduit to facilitate nucleocytoplasmic transport in eukaryotes. The asymmetric distribution of the small G protein Ran across the nuclear envelope regulates directionality of protein transport. Ran interacts with the NPC of metazoa via two asymmetrically localized components, Nup153 at ...

A 73-year-old man, a right-handed, has been pointed out his atrial fibrillation and seen a doctor regularly for varicose veins of left leg and hypertension. He had complaint of a sudden paralysis of his left thumb and index finger while drinking beer. The next day, there was no improvement and he was admitted to our hospital. Neurological examination ...

Rds3p, a component of the U2 snRNP subcomplex SF3b, is essential for pre-mRNA splicing and is extremely well conserved in all eukaryotic species. We report here the solution structure of Rds3p, which reveals an unusual knotted fold unrelated to previously known knotted proteins. Rds3p has a triangular shape with a GATA-like zinc finger at each vertex. ...

We report a case of 2-staged reconstruction of flexor tendons ruptured spontaneously by attrition. A 49-year-old man presented with inability to flex the ring and little fingers of his left hand. Preoperative computed tomographic scans revealed fracture of the hamate hook. At operation, both the flexor superficialis and profundus of the little ...

We present a case of a 64-year-old man from Belgian origin with a chronic painful swelling of the third finger. A chronic arthritis from atypical germ was suspected at the third metacarpo-phalangeal joint on the plain radiography, ultrasonography and MR examination. A few months later, he developed thoracic back pain. Plain radiographs, a Technetium-99m bone scan and MR ...

Robotic hands built for manipulation are often anthropomorphic but not anatomically accurate. We are constructing an anatomically-correct testbed (ACT) of the human hand to understand its mechanisms, function, and control. We have previously demonstrated that an accurate model of the extensor mechanism in the ACT Hand is crucial in realizing the human-like finger movements. ...

Object manipulation is one crucial task in robotics. This chapter presents a precision control scheme of a multi-fingered humanoid robot arm based on tactile sensing information to perform object manipulation tasks. We developed a novel optical three-axis tactile sensor system mounted on the fingertips of a humanoid robot to enhance its ability to recognize and manipulate ...

The brain's ability to synchronize movement with external cues is used daily, yet neuroscience is far from a full understanding of the processes that allow these simple sequential performances. This experimental design was implemented to determine differences in brain activity when finger tapping at increasing and decreasing rhythms. Eight subjects tapped their right index ...

A granular instability driven by gravity is studied experimentally and numerically. The instability arises as grains fall in a closed Hele-Shaw cell where a layer of dense granular material is positioned above a layer of air. The initially flat front defined by the grains subsequently develops into a pattern of falling granular fingers separated by rising bubbles of air. A ...

A granular instability driven by gravity is studied experimentally and numerically. The instability arises as grains fall in a closed Hele-Shaw cell where a layer of dense granular material is positioned above a layer of air. The initially flat front defined by the grains subsequently develops into a pattern of falling granular fingers separated by rising bubbles of air. A ...

A granular instability driven by gravity is studied experimentally and numerically. The instability arises as grains fall in a closed Hele-Shaw cell where a layer of dense granular material is positioned above a layer of air. The initially flat front defined by the grains subsequently develops into a pattern of falling granular fingers separated by rising bubbles of air. A ...

A granular instability driven by gravity is studied experimentally and numerically. The instability arises as grains fall in a closed Hele-Shaw cell where a layer of dense granular material is positioned above a layer of air. The initially flat front defined by the grains subsequently develops into a pattern of falling granular fingers separated by rising bubbles of air. A ...

Our experiments on viscous fingering of air into oil contained between closely spaced plates reveal two selection rules for the fjords of oil that separate fingers of air. (Fjords are the building blocks of solutions of the zero-surface-tension Laplacian growth equation.) Experiments in rectangular and circular geometries yield fjords ...

Recently a fingering morphology, resembling the hydrodynamic Saffman-Taylor instability, was identified in the quasi-two-dimensional electrodeposition of copper. We present here measurements of the dispersion relation of the growing front. The instability is accompanied by gravity-driven convection rolls at the electrodes, which are examined using particle image velocimetry. ...

The bfp, a member of the RING finger family, has been shown to be predominantly expressed in brain and up-regulated in neural differentiation of P19 embryonic carcinoma cells. Chromosome mapping of the bfp gene by fluorescence in situ hybridization reveals that human BFP (ZNF179) is located at 17p11.2, mouse Bfp at 11B1.3, and rat BFP at 10q22. These ...

Non-invasive beat-to-beat mean arterial pressure (MAP) in finger arteries recorded by the differential oscillometric device was compared with MAP recorded invasively from A. radialis in 22 patients after cardiac surgery. Based on all 132 paired measurements, the MAP values measured at the radial artery were 2.7?�?4.9?mmHg higher than those measured on ...

We present a 20-year-old Malay male whom we believe has Proteus syndrome, a rare congenital disorder of asymmetrical overgrowth of body tissues. There are fewer than 100 confirmed cases reported worldwide thus the clinical presentation and histopathological findings are of significance. Our patient presented with an overgrown right small finger and subcutaneous purplish ...

Poly(ADP-ribosyl)ation represents an important post-translational modification in higher eukaryotes. Several DNA repair/checkpoint proteins possess specific PAR-Binding Zinc finger (PBZ) modules critical for function. Here, we present solution structures of the two PBZ modules of APLF (Aprataxin and PNK-like factor), revealing a novel type of zinc ...

A pair of concentric spheres separated by a small gap form a spherical Hele-Shaw cell. In this cell an interfacial instability arises when two immiscible fluids flow. We derive the equation of motion for the interface perturbation amplitudes, including both pressure and gravity drivings, using a mode coupling approach. Linear stability analysis shows that mode growth rates depend upon interface ...

A 48-year-old woman presented with acute unilateral ischaemia of the left hand. She had a background of chronic peripheral neuropathic pain, palpitations, anaemia and an episode of superficial thrombophlebitis. Physical examination revealed non-blanching purple discoloration of her left fingers and her left thumb, index finger and ...

The A-site-ordered perovskite YMn(3)Al(4)O(12) was prepared by high-pressure synthesis. Structural analysis with synchrotron powder X-ray diffraction data and the Mn L-edges X-ray absorption spectrum revealed that the compound has a chemical composition Y(3+)Mn(3+)(3)Al(3+)(4)O(2-)(12) with magnetic Mn(3+) at the A' site and ...

Cys₂His₂ zinc finger proteins make up the largest class of transcription factors encoded in the genomes of higher eukaryotes. Recent studies of the Ikaros transcription factor demonstrated that this zinc finger protein undergoes cell cycle-dependent changes in association with DNA that seem to be due to ...

Biometrics performs personal authentication using individual bodily features including fingerprints, faces, etc. These technologies have been studied and developed for many years. In particular, fingerprint authentication has evolved over many years, and fingerprinting is currently one of world's most established biometric authentication techniques. Not long ago this technique was only used for ...

PurposeTo develop a dedicated RF coil for high-resolution MR imaging of finger joints at 3T to improve diagnostic evaluation of arthritic diseases.Materials and MethodsA dedicated cylindrical RF receive coil was developed for imaging finger joints at 3T. A planar coil, a saddle coil and a 1.5T coil with similar design as the dedicated coil were also ...

DNA structural changes such as bending play an important role in various biological reactions. Not only protein binding to its specific DNA sequence but also DNA bending induced by the protein is indispensable for unique gene expression. Therefore, an artificial protein that induces a DNA conformational change is interesting as a transcriptional regulator of a specific gene. We created 6-zinc ...

Little is known about the contribution of individual zinc-ligating amino acid residues for coupling between zinc binding and protein folding in zinc finger domains. To understand such roles of each zinc ligand, four zinc finger mutant peptides corresponding to the second zinc finger domain of Sp1 were synthesized. In the mutant ...

The purpose of this study was to evaluate the flexibility, shape, and surface finishing of stainless steel (SS) and nickel-titanium (NiTi) finger spreaders as well as to compare the load required to insert these spreaders along a gutta-percha point adapted to the apical segment of curved or straight artificial canals. Instrument flexibility was investigated by using a ...

A minimalist Cys2His2 zinc finger peptide, Lys-Tyr-Ala-Cys-Ala-Ala-Cys-Ala-Ala-Ala-Phe-Ala-Ala-Lys-Ala-Ala-Leu-Ala- Ala-His-Ala-Ala-Ala-His-Ala-Lys, has been synthesized. Metal binding studies using Co2+ as a probe indicated that this peptide forms a 1:1 peptide/metal complex with a dissociation constant comparable to that observed for other zinc finger ...

comprises a cuff, a stem, a housing, a first finger, a second finger, a means for biasing the fingers towards each other, and a brace. ...

... splints used to treat mallet finger. A, Dorsal aluminum splint. B, Commercial splint. Reproduced with permission from ...

Development of more useful screening systems for anti cancer drugs is important to accelerate efficiency of finding new leads. The National Cancer Institute (NCI) has recently developed a unique screening methodology using a human cell line panel. We have established a new human cell line panel according to this news methodology, and studied its feasibility. Concentration of a certain sample to ...

Recent studies have described muscle synergies as overlapping, multimuscle groups defined by synchronous covariation in activation intensity. A different approach regards a synergy as a fixed temporal sequence of bursts of activity across groups of motoneurons. To pursue this latter definition, the present study used a principal component (PC) analysis tailored to reveal the ...

We studied a relation of chaotic dynamics of finger plethysmogram to complexity of high cerebral center in both theoretical and experimental approaches. We proposed a mathematical model to describe emergence of chaos in finger tip pulse wave, which gave a theoretical prediction indicating increased chaoticity in higher cerebral center leading to an ...

Apparatus constructed to measure the variation (?V) in applied potential (V) over the surface of brass discs subject to corrosion by latent finger mark deposits is described. Results show values of ?V of up to 14 V for V = 1400 V on regions of disc coincident with the location of finger mark corrosion. Measurement of ?V for 0 V <= V <= 1400 V has ...

The venom of the sea krait, Laticauda semifasciata, consists primarily of two toxic proteins, phospholipase A(2) (PLA(2)) and a three-finger-structure toxin. We have cloned both toxic protein genes, including the upstream region. PLA(2) genes contain three types of inserted sequences: an AG-rich region, a chicken repeat 1-like long interspersed nucleotide element sequence and ...

A suppression subtraction hybridization (SSH) cDNA library had been constructed to identify differentially expressed genes. Based on the sequence of an expressed sequence tag (EST) homologous to Pisum sativum zinc finger protein mRNA (Accession number: AF160911), the full-length cDNA of 1,676 nucleotides was cloned from alfalfa by rapid amplification of cDNA ends (RACE). It ...

An instability is observed as a layer of dense granular material positioned above a layer of air falls in a gravitational field [Phys. Rev. Lett. 99, 048001 (2007)]. A characteristic pattern of fingers emerges along the interface defined by the grains, and a transient coarsening of the structure is caused by a coalescence of neighboring fingers. The ...

It is well known that timing of rhythm production is disrupted by delayed auditory feedback (DAF), and that disruption varies with delay length. We tested the hypothesis that disruption depends on the state of the movement trajectory at the onset of DAF. Participants tapped isochronous rhythms at a rate specified by a metronome while hearing DAF (for piano tones) of differing lengths. Motion ...

A cDNA clone has been prepared from mRNA of cultured human keratinocytes. The sequence of the cDNA reveals that in the C-terminal two-thirds of the corresponding protein (basonuclin), there are three separated pairs of adjacent zinc fingers. The amino acid sequence of each pair is homologous to that of the single pair of zinc fingers ...