... ethanol. The silicone membrane was clamped between poly-acetal plates and autoclaved at 120°C for 20 ... ...
NBII National Biological Information Infrastructure
... Secondary cultures, created by addition of Vi-Bacteriophages to strains containing Vi and O-Antigen, are not agglutinable and have a smooth ...
DTIC Science & Technology
Proliferation of vascular smooth muscle cells with the accumulation of proteoglycans in the extracellular matrix is one of the significant changes found in atherosclerotic lesions. In order to clarify the relationship between pericellular proteoglycan and cell growth, we established a simple method for quantitatively estimating the amount of pericellular proteoglycans and ...
PubMed
... the endometrium plus the smooth muscle in the myometrium. These two tissues are regulated differently by steroid ... sarafotoxin 6c (S6c)�have been identified in human myometrium, but we and others have d...
Hydrolysis of exogenous adenine nucleotides by human cultured endothelial and smooth muscle cells and fibroblasts has been compared. Endothelial cells and fibroblasts readily degrade exogenous ATP or ADP to adenosine and inosine, while smooth muscle cells...
National Technical Information Service (NTIS)
Several different studies have investigated the growth effects of angiotensin II on vascular smooth muscle cells in culture. However, smooth muscle cells change their phenotype when placed in culture. The objective of the present study was to investigate the effects of angiotensin II on (3)H-thymidine and ...
In vitro studies were carried out to induce viral transformation of vascular smooth muscle cells. Cultured rabbit arterial smooth muscle cells were infected with simian virus 40 (SV 40), and transformed cultures were produced that exhibit altered morphology, increased growth rate and plating efficiency, growth on ...
Cardiovascular diseases (CVDs) result from the sub-endothelial accumulation of inflammatory cells and smooth muscle cells (SMCs). Lycopene, a natural compound from tomato, has been suggested to play a role in CVD prevention. However, its action mechanism is still largely unknown. In this study, we examined the effect of lycopene on SMCs. We found that preincubation of PDGF-BB ...
Cultured smooth muscle cells (A-10), derived from rat thoracic aorta, have vascular (V/sub 1/) vasopressin receptors. They have previously shown that these receptors mediate phosphatidylinositol turnover, Ca/sup 2 +/ efflux, and inhibition of isoproterenol-induced increases in cAMP. Here they studied the effect of ...
Energy Citations Database
The proliferation of vascular smooth muscle cells (VSMC) is a crucial pathophysiological process in the development of atherosclerosis. Although estrogen is known to inhibit the proliferation of VSMC, the mechanism responsible for this effect remains to be elucidated. In addition, the effect of raloxifene on VSMC remains unknown. We have shown here that 17beta-estradiol (E(2)) ...
