A humanised IgG1/k version of A33 (hA33) has been constructed and expressed with yields up to 700 mg l-1 in mouse myeloma NS0 cells in suspension culture. The equilibrium dissociation constant of hA33 (KD = 1.3 nM) was shown to be equivalent to that of the murine antibody in a cell-binding assay. hA33 labelled with ...
PubMed Central
Despite progress made over the past 25 years, existing immunotherapies have limited clinical effectiveness in patients with cancer. Immune tolerance consistently blunts the generated immune response, and the largely solitary focus on CD8+ T cell immunity has proven ineffective in the absence of CD4+ T cell help. To address these twin-tier deficiencies, we developed a translational model of ...
PubMed
Despite progress made over the past 25 years, existing immunotherapies have limited clinical effectiveness in patients with cancer. Immune tolerance consistently blunts the generated immune response, and the largely solitary focus on CD8+ T cell immunity has proven ineffective in the absence of CD4+ T cell help. To address these twin-tier deficiencies, ...
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NASA Website
An anti-poxvirus vaccine based on replicon particles of Venezuelan equine encephalitis virus (VRP) is being developed. The cowpox virus genes encoding structural proteins corresponding to vaccinia virus proteins A33, B5, and A27 were each expressed from VRP. High serum IgG titers against these proteins were generated in BALB/c mice ...
The pag gene coding for protective antigen (PA), one of the three toxin components of Bacillus anthracis, has been cloned into the mobilizable shuttle vector pAT187 and transferred by conjugation from Escherichia coli to B. anthracis. Using this strategy, an insertionally mutated pag gene constructed and characterized in E. coli, was introduced into B. anthracis Sterne strain. This transconjugant ...
The monoclonal antibody A33 recognises a tumour-associated antigen on human colorectal carcinoma, and has undergone preliminary evaluation in the clinic where selective localisation to hepatic metastases has been demonstrated [Welt et al. (1994) J. Clin. Oncol. 12, 1561-1571]. A33 and an A33 tri-fab fragment (TFM) ...
Description: USA-NEW YORK/ADIRONDACK MOUNTAINS STS61A-33-45 UNITED STATES OF AMERICA, NEW YORK/ADIRONDACK MOUNTAINS. ID: STS61A-33-45 ...
Although the prevalence of Bordetella parapertussis varies dramatically among studies in different populations with different vaccination regimens, there is broad agreement that whooping cough vaccines, composed only of B. pertussis antigens, provide little if any protection against B. parapertussis. In C57BL/6 mice, a B. pertussis whole-cell vaccine (wP) provided modest ...
Three distinct chimpanzee Fabs against the A33 envelope glycoprotein of vaccinia virus were isolated and converted into complete monoclonal antibodies (MAbs) with human gamma 1 heavy-chain constant regions. The three MAbs (6C, 12C, and 12F) displayed high binding affinities to A33 (K(d) of 0.14 nM to 20 nM) and may recognize the same epitope, which was determined to be conformational and located ...
Three distinct chimpanzee Fabs against the A33 envelope glycoprotein of vaccinia virus were isolated and converted into complete monoclonal antibodies (MAbs) with human ?1 heavy-chain constant regions. The three MAbs (6C, 12C, and 12F) displayed high binding affinities to A33 (Kd of 0.14 nM to 20 nM) and may recognize the same epitope, which was determined to be ...
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Host immunity is a major driving force of antigenic diversity, resulting in pathogens that can evade immunity induced by closely related strains. Here we show that two Bordetella bronchiseptica strains, RB50 and 1289, express two antigenically distinct O-antigen serotypes (O1 and O2, respectively). When 18 additional B. bronchiseptica strains were serotyped, all were found to express either the O1 ...
Immunization with recombinant proteins may provide a safer alternative to live vaccinia virus for prophylaxis of poxvirus infections. Although antibody protects against vaccinia virus infection, the mechanism is not understood and the selection of immunogens is daunting as there are dozens of surface proteins and two infectious forms known as the mature virion (MV) and the enveloped virion (EV). ...
Protein-based subunit smallpox vaccines have shown their potential as effective alternatives to live virus vaccines in animal model challenge studies. We vaccinated mice with combinations of three different vaccinia virus (VACV) proteins (A33, B5, L1) and examined how the combined antibody responses to these proteins cooperate to ...
The spread of vaccinia virus in cell cultures is mediated by virions that adhere to the tips of specialized actin-containing microvilli and also by virions that are released into the medium. The use of a small plaque-forming A36R gene deletion mutant to select spontaneous second-site mutants exhibiting enhanced virus release was described previously. Two types of mutations were found: C-terminal ...
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Date: 11.10.1984. Title: PUERTO RICO/COMPLETE ISLAND. Description: PUERTO RICO/ COMPLETE ISLAND STS51A-33-103 PUERTO RICO/COMPLETE ISLAND. ID: STS51A-33-103 ...
Brucella species are Gram-negative, facultative intracellular bacteria that cause zoonotic brucellosis. Survival and replication inside macrophages is critical for establishment of chronic Brucella infection. Virulent smooth B. abortus strain 2308 inhibits programmed macrophage cell death and replicates inside macrophages. Cattle B. abortus vaccine strain RB51 is an attenuated rough, ...
Ethylene glycol alkyl ethers are frequently used in industry, and accidents due to them occur. Impaired hematopoietic function and genital injury in animal experiments have been reported. Of various alkyl radicals, those with a methyl radical strongly injure them. Ethylene glycol dimethyl ether (EGDME) was administered to pregnant mice on the 7th, 8th, 9th, and 10th days of ...
The biological threat imposed by orthopoxviruses warrants the development of safe and effective vaccines. We developed a candidate orthopoxvirus DNA-based vaccine, termed 4pox, which targets four viral structural components, A33, B5, A27, and L1. While this vaccine protects mice and nonhuman primates from lethal infections, we are ...
... Cuadernos del Instituto de Biolog�a 33: Cat�logo de angiospermas acu�ticas de M�xico; hidr�fitas estrictas emergentes, sumergidas y ... Cuadernos del Instituto de Biolog�a 33: Cat�logo de angiospermas acu...
NBII National Biological Information Infrastructure
Table 33 includes the mean, standard error of the mean, and percentiles of the distribution of intake; Appendix Table A33 provides percentile values (rounded) and their standard errors.
Cancer.gov
... t _t t ft Fig. ... Materials Science and Engineering A] 33 (1991 ) 1. [9]. RC O'Handley, Modern Magnetic Materials, John Wiley & Sons, New York (2000 ...
DTIC Science & Technology
Bounded on either side by the Canadian province of Quebec, the Saint Lawrence River is the connecting link for shipping traffic between the Atlantic Ocean ...
... Lake George (right center), and numerous small rivers and creeks draining from the Adirondack Mountains to the west and the Green Mountains to the east. ...
Department of Botany and Plant Pathology. Colorado State University. Fort Collins, Colorado 80523, U.S.A.. 33. Page 2. ABSTRACT ...
STS61A-33-0045 Adirondack Mountains, Lakes George and Great Sacandaga, New York ... The southeastern Adirondack Mountains cover the majority of the lower ...
... Properties for PBO 8A 33 13 Compression Tests PBO 8A 35 ... 36 15 Machine Compliance Curve for PBO 8A: Compression Tests 37 ...
at the mudline, where rising pore fluids interact with seawater to produce carbonates and rare zeolites (analcime
E-print Network
for each structure t~ cctablish a state sf repair and overall ... fect of sonic bocms on the hatchability of chicken eggs; human ...
The Department of Agriclture, Statistical Reporting Service, has. 17, 000 sampling units (one square mile each) scattered over the United. States. ...
Naturally occurring smallpox was eradicated as a result of successful vaccination campaigns during the 1960s and 1970s. Because of its highly contagious nature and high mortality rate, smallpox has significant potential as a biological weapon. Unfortunately, the current vaccine for orthopoxviruses is contraindicated for large portions of the population. Thus, there is a need for new, safe, and ...
Naturally occurring smallpox was eradicated as a result of successful vaccination campaigns during the 1960s and 70s. Because of its highly contagious nature and high mortality rate, smallpox has significant potential as a biological weapon. Unfortunately, the current vaccine for orthopoxviruses is contraindicated for large portions of the population. Thus, there is a need for new, safe, and ...
Goatpox, caused by goatpox virus (GTPV), is an acute feverish and contagious disease in goats often associated with high morbidity and high mortality. To resolve potential safety risks and vaccination side effects of existing live attenuated goatpox vaccine (AV41), two Semliki forest virus (SFV) replicon-based bicistronic expression DNA vaccines (pCSm-AAL and pCSm-BAA) which encode GTPV structural ...
Energy Citations Database
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and Eurasian plates in the Pyrenean domain during the opening of the Bay of Biscay, from chrons M0 to A33o time cause? Seafloor spread- ing in the Bay of Biscay stopped soon after chron A33o (old), as demonstrated strong evidence that seafloor spreading had stopped in the Bay of Biscay soon after chron A33o
Isotopes which emit low energy electrons deposit a decreasing gradient of local energy as a function of distance from the isotope. Therefore if antibodies internalize and transport such isotopes to close proximity of the nucleus, an additional therapeutic advantage will be realized. We studied the subcellular distribution of a internalizing antibody, A33, ...
Orthopoxviruses produce two, antigenically distinct, infectious virions, intracellular mature virions and extracellular virions (EV). A33 and B5 are found on EV but not intracellular mature virions. To investigate the function of A33, a recombinant virus that has A33R deleted and expresses B5R-GFP ...
Protein A33 is a type II membrane protein present in the outer envelope of extracellular as well as cell-associated Vaccinia virus particles. A33 has been implicated in mediating cell-to-cell virus spread in an antibody-resistant manner. Here, using state-of-the-art structure prediction methods and structural ...
