anchoring protein AKAP, protein kinase C, and protein phosphatases 1 and 2A. Beta-adrenergic medi- ated

AAAA @@@AAAAAAA@@@AA AAAA@@AAA@@@AAAA CCCCCCAABBBBBBBB@@@ AACCCA@@@@BBBBBBBBC ..... AAAAABBBBBBBBBBBBBBAABCCCCCCCDEECBCDDDDB@@AAA@@AAA@??????@@@? ...

... eq 4 ae a * aaa aa 13 B. The prevention of Kiting Damage aaaa * aaaa 13 C. Dynamioe of a Kiting Airship .. . . a. . . a a aaaa aa 2 ...

Four DNA-dependent ATPases have been purified to apparent homogeneity from extracts of the Novikoff Hepatoma, and named ATPases II, III, IV, and V. The physical and enzymological properties of ATPases II, III, and V are nearly identical, and from tryptic peptide mapping these proteins were determined to be related, ...

9: 88635534533342421221111122222333444544543455543333467755643100235546666667777799777767543553333333469 :SLECA@AAAA@>=><==;;;:<<<=<< ...

... A Appendix A to Subpart AAAA of Part 97 Protection of Environment ENVIRONMENTAL...Trading Program General Provisions Pt. 97, Subpt. AAAA, App. A Appendix A to Subpart AAAA of Part 97�States With Approved State...

... A Appendix A to Subpart AAAA of Part 97 Protection of Environment ENVIRONMENTAL...Trading Program General Provisions Pt. 97, Subpt. AAAA, App. A Appendix A to Subpart AAAA of Part 97�States With Approved State...

The influence of plasma membrane lipid components on the activity of the H{sup +}-ATPase has been studied by determining the effect of surfactants on membrane lipids and ATPase activity of oat (Avena sativa L.) root plasma membrane vesicles purified by a two-phase partitioning procedure. Triton X-100, at 25 to 1 (weight/weight) Triton to plasma membrane ...

This proposal was to study the structure and function of the yeast plasma membrane H(+)-ATPase. We proposed to study (1) the kinetics of proton pumping, (2) the transmembrane distribution of the protein and (3) the interaction between the ATPase and an as...

The objective of this two year project was to identify protein structure domains participating in proton transport and membrane voltage interactions by the plasma membrane H(+)-ATPase from Saccharomyces cerevisiae. H+-ATPase mutants (pmal) were generated ...

The purified bovine brain ATPase has been shown to be a high-affinity, low capacity pump, and consists of two proteins. A labile, high affinity dihydropyridine receptor activity has been associated with this ATPase activity. This study has shown that the ...

To understand the importance of selected regions of the regulatory light chain (RLC) for phosphorylation-dependent regulation of smooth muscle myosin (SMM), we expressed three heavy meromyosins (HMMs) containing the following RLC mutants; K12E in a critical region of the phosphorylation domain, GTDP(95-98)/AAAA in the central hinge, and R160C a putative binding residue for ...

To understand the importance of selected regions of the regulatory light chain (RLC) for phosphorylation-dependent regulation of smooth muscle myosin (SMM), we expressed three heavy meromyosins (HMMs) containing the following RLC mutants; K12E in a critical region of the phosphorylation domain, GTDP^95-98/AAAA in the central hinge, and R160C a putative ...

This progress report discusses the molecular mechanism of energy transduction by plant membrane proteins. Topics include the location of the DCCD-reactive glutamate in the ATP-ase, purification and sequencing of tryptic peptides, inhibition and labeling of the oat root plasma membrane ATP-ase, an immunological analysis of the plasma ...

is a transmembrane domain that can couple to socalled energycoupling modules [38]. In ABC transporters, extra. It is a main source of metabolic energy in living cells, fuelling transport (via transport ATPases binding cassette (ABC) transport ATPases has been the focus of this thesis and these proteins

... was to study, on the cellular level, the amount and composition of RNA, the amount of proteins, res piratory enzyme and ATP-ase activities during ...

... By means of spectral luminescence and ATP-ase activity measurements recordings were made of conformation transitions in myofibrillar proteins ...

The plasma membrane Ca2+ -ATPase protein from red blood cells is not modified in preeclampsia during preeclampsia. We investigated whether the number of Ca2+ -ATPase molecules is modified in red cell, suggesting that the protein structure remains intact in preeclampsia. Calmodulin, ethanol, or both calmodulin

aggregation occurred. At higher melittin/protein molar ratios (10:1 and 30:1), the inhibition of the enzyme . In purified preparations of Na,K-ATPase, the maximal aggregation (about 90%) was achieved at a melittin/protein.V. All rights reserved. Keywords: Melittin; Protein aggregation; ...

Solubilization of microsomal proteins followed by calmodulin affinity chromatography resulted in the separation of two distinct Ca2+-Mg2+-ATPases (Ca2+-regulated Mg2+-dependent ATPases), one being insensitive to calmodulin (ATPase-1), the other being stimulated about 5-fold by calmodulin ...

Na- and K-dependent ATPase (Na,K-ATPase) in the basolateral membrane of corneal endothelial cells plays an important role in the pump function of the corneal endothelium. We investigated the role of dexamethasone in the regulation of Na,K-ATPase activity and pump function in these cells. Mouse corneal endothelial cells were exposed to ...

... `aaaa````aaaa````````__`abcccbbbb````````_____`bcaabcccbbaaaa``bcbbbba`]b^^^ _`a`acdddbbcdeedcbbaaa`_\\XXZ\\`bcc``abcdddccccccccbbcdfghijjjifeccdca_\\]dgig` ...

1. The specific activity of brain (Na+ + K+)-ATPase and Mg2+ -ATPase of the ground squirrel (Spermophilus richardsonii) is significantly increased after long-term hibernation. 2. The markedly non-linear thermal dependence of (Na+ + K+)-ATPase is unchanged during hibernation whereas the near linear thermal dependence of ...

P4 ATPases (subfamily IV P-type ATPases) form a specialized subfamily of P-type ATPases and have been implicated in phospholipid translocation from the exoplasmic to the cytoplasmic leaflet of biological membranes. Pivotal roles of P4 ATPases have been demonstrated in eukaryotes, ranging from yeast, fungi and ...

The molecular weight and isoelectric point of the plasma membrane H/sup +/-ATPase from red beet storage tissue were determined using N,N'-dicyclohexylcarbodiimide (DCCD) and a H/sup +/-ATPase antibody. When plasma membrane vesicles were incubated with 20 micromolar (/sup 14/C)-DCCD at 0/sup 0/C, a single 97,000 dalton protein ...

... Accession Number : ADA107162. Title : Effect of Antibody to Anisole Binding Protein on Odorant Perturbation of Na(+) -K(+) ATPase Activity,. ...

Investigation the influence of calyx[4]arenes C-90, C-91, C-97 and C-99 (codes are indicated) on the enzymatic activity of four functionally different Mg2+ -dependent ATPases from smooth muscle of the uterus: actomyosin ATPase, transporting Ca2+, Mg2+ -ATPase, ouabain-sensible Na+, K+ -ATPase and basal Mg2+ ...

By trypsin treatment of highly purified ATPase (EC 3.6.1.3) from Micrococcus sp. ATCC 398E, two enzyme modifications have been obtained. (i) ATPase Ta, which has about the same activity as untreated ATPase. (ii) A protein complex Ti, which lacks ATPase activity, but nevertheless binds ATP as ...

Potato mitochondria (Solanum tuberosum var. Russet Burbank), which readily phosphorylate ADP in oxidative phosphorylation, show low levels of ATPase activity which is stimulated neither by Mg/sup 2 +/, 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with Triton X-100, individually or in concert. Treatment of disrupted potato ...

Potato mitochondria (Solanum tuberosum var. Russet Burbank), which readily phosphorylate ADP in oxidative phosphorylation, show low levels of ATPase activity which is stimulated neither by Mg²⁺, 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with Triton X-100, individually or in concert. Treatment of ...

... 4 Table 4 of Subpart AAAA to Part 60 Protection of Environment ENVIRONMENTAL...PERFORMANCE FOR NEW STATIONARY SOURCES Pt. 60, Subpt. AAAA, Table 4 Table 4 of Subpart AAAA to Part 60�Requirements for Continuous Emission...

... 3 Table 3 of Subpart AAAA to Part 60 Protection of Environment ENVIRONMENTAL...PERFORMANCE FOR NEW STATIONARY SOURCES Pt. 60, Subpt. AAAA, Table 3 Table 3 of Subpart AAAA to Part 60�Requirements for Validating...

The Na-K-ATPase is an energy-transducing ion pump that converts the free energy of ATP into transmembrane ion gradients. It also serves as a functional receptor for cardiotonic steroids such as ouabain and digoxin. Binding of ouabain to the Na-K-ATPase can activate calcium signaling in a cell-specific manner. The exquisite calcium modulation via the ...

A Mg(2+)-ATPase was solubilized from membranes of Acetabularia cliftonii using nonanoyl-N-methylgluconamide and purified by ion-exchange and gel permeation chromatography. One active ATPase fraction after Mono Q chromatography had a specific activity of 10 units/mg of protein. Judged from subunit composition [54 (a), 50 (b) with a ...

The inositol phospholipids, phosphatidylinositol monophosphate (PIP) and phosphatidylinositol bisphosphate (PIP{sub 2}), have been shown to increase the vanadate-sensitive ATPase activity of plant plasma membranes. In this paper, the authors show the effect of various concentrations of phosphatidyinositol, PIP, and PIP{sub 2} on the plasma membrane vanadate-sensitive ...

The morphological structure (pulvinus P1, P2 and P3) directly involved in the seismonastic movements of the Mimosa pudica leaf have been used to isolate: 1) "soluble" ATPase, loosely bound to pulvinus structures; 2) Ca, Mg-dependent ATPase, which is tightly bound to pulvinus structures and is extracted by a saline solution of high ionic strength, used to ...

1 ATPase activities were studied in erythrocyte membranes prepared from blood of patients suffering from affective disorders. 2 Long-term (9-12 months) administration of lithium led to an increase in the erythrocyte membrane Na/K ATPase activity (54%) when studied on an age and sex matched basis or when the patients were studied before and after treatment. ...

Our previous study showed that the recombinant ATPase encoded by the A32L gene of orf virus displayed ATP hydrolysis activity as predicted from its amino acids sequence. This viral ATPase contains four known functional motifs (motifs I-IV) and a novel AYDG motif; they are essential for ATP hydrolysis reaction by binding ATP and magnesium ions. The motifs I ...

The activity of the membrane-bound H⁺-ATPase of the beer spoilage bacterium Lactobacillus brevis ABBC45 increased upon adaptation to bacteriostatic hop compounds. The ATPase activity was optimal around pH 5.6 and increased up to fourfold when L. brevis was exposed to 666 ?M hop compounds. The extent of activation depended on the ...

The Co-chaperone p23 Arrests the Hsp90 ATPase Cycle to Trap Client Proteins Stephen H. McLaughlin1 by conformational changes driven by ATP binding and hydrolysis. Association of co-chaperones and client proteins. Keywords: molecular chaperone; Hsp90; heat shock; protein folding; p23*Corresponding authors ...

Mutations disrupting helicase domain motifs of the Tobacco mosaic virus 126/183-kDa proteins were investigated for their effect on replicase function and assembly. These mutations inhibited virus replication but did not affect 126-kDa induced N gene resistance or RNAi suppression. However, in vivo expressed 126-kDa motif mutants yielded two distinct cytoplasmic phenotypes that ...

The response of sodium, potassium-adenosine triphosphatase (Na,K-ATPase) to cyclic adenosine monophosphate (cAMP)-dependent protein kinase was examined in membranes obtained from rabbit iris-ciliary body. In the presence of the protein kinase together with 10(-5) M cAMP, Na,K-ATPase activity was reduced. No change ...

The artificial regulation of protein functions is essential for the realization of protein-based soft devices, because of their unique functions conducted within a nano-sized molecular space. We report that self-assembled nanomeshes comprising heat-responsive supramolecular hydrogel fibers can control the rotary motion of an enzyme-based biomotor ...

The plant vacuole is acidified by a complex multimeric enzyme, the vacuole-type H⁺-ATPase (V-ATPase). The initial association of ATPase subunits on membranes was studied using an in vitro assembly assay. The V-ATPase assembled onto microsomes when V-ATPase subunits ...

Curcumin is an important nutraceutical widely used in disease treatment and prevention. We have previously suggested that curcumin interferes with K(+) binding to pig kidney Na,K-ATPase by interaction with its extracellular domains. The aim of this study was to further characterize the site of curcumin interaction with the ATPase. We have performed pair ...

A comparison has been made of the developmental gradients along a mung bean (Vigna radiata L.) hypocotyl of the growth rate, plasma membrane ATPase, and fusicoccin-binding protein (FCBP) activity to determine whether they are interrelated. The hook and four sequential 7.5 millimeter segments of the hypocotyl below the hook were cut. A plasma ...

Age changes in the subunits composition were comparatively studied for protein, ATPase activity, sensitivity to Ca2+ in low concentrations and alkaline activation of ATPase of natural actomyosin of skeletal muscles and myocardium in cattle feti. A rise in values in the process of the mentioned parameters development is established for ...

ABSTRACT The vacuolar H ?-ATPases (V-ATPases) are a universal class of proton pumps that are structurally similar to the F-ATPases. Both protein families are characterized by a membrane-bound segment (V o,F o) responsible for the translocation of protons, and a soluble portion, (V 1,F 1), which supplies the energy ...

Na-K-ATPase activity was measured with an ultramicromethod in single portions of the proximal and distal convolution and of the thick ascending limb of Henle from adrenalectomized rats and after treatment with 5 mug aldosterone per 100 g body wt. The activity in all tubular structures returned to normal within 1 h after injection. This rapid activation of ...

Membrane vesicles and the F/sub 1/-Atpase from Clostridium thermoaceticum were examined by electron microscopy. F/sub 1/-ATPase particles projecting from the vesicles have a diameter of 10 to 12 nm. The F/sub 1/-ATPase has an..cap alpha../sub 3/..beta../sub 3/..gamma..delta structure. The ..cap alpha.. and ..beta.. subunits are most ...

1. Hybrid or reconstituted troponins were prepared from troponin components of rabbit skeletal muscle and porcine cardiac muscle and their effect on the actomyosin ATPase activity was measured at various concentrations of Ca2+ or Sr2+. The Ca2+ concentration required for half-maximum activation of actomyosin ATPase with troponin containing cardiac troponin ...

An energy-transducing adenosine triphosphatase (ATPase, EC 3.6.1.3) that contains an extra polypeptide (delta) as well as three intrinsic subunits (alpha, beta, gamma) was purified from Micrococcus lysodeikticus membranes. The apparent subunit stoichiometry of this soluble ATPase complex is alpha 3 beta 3 gamma delta. The functional role of the subunits ...

Short term effects of insulin on total brain and branchial Na+K+ ATPase, Ca2+ ATPase and Na+, K+ and Ca2+ ions were investigated in A. testudineus. The increase in brain Ca2+ ATPase after alloxan treatment may account for an increased amount of intracellular calcium required for biochemical events taking place inside the cells. ...

The ATPase Vps4 belongs to the type-I AAA family of proteins. Vps4 functions together with a group of proteins referred to as ESCRTs in membrane deformation and fission events. These cellular functions include vesicle formation at the endosome, cytokinesis and viral budding. The highly dynamic quaternary structure of Vps4 and its ...

Stomatal opening, which is mediated by blue light receptor phototropins, is driven by activation of the plasma membrane H(+)-ATPase via phosphorylation of the penultimate threonine in the C-terminus and subsequent binding of a 14-3-3 protein. However, the biochemical properties of the protein kinase and protein ...

P-type ATPases are a superfamily of membrane proteins involved in many physiological processes that are fundamental for all living organisms. Using ATP, they can transport a variety of ions and other substances across all types of cell membranes against a concentration electrochemical gradient. P-type ATPases form a phosphorylated ...

The monoclonal antibody to the beta-subunit of H(+)/K(+)-ATPase (mAbHKbeta) cross-reacts with a protein that acts as a molecular chaperone for the structural maturation of sarcoplasmic reticulum (SR) Ca(2+)-ATPase. We partially purified a mAbHKbeta-reactive 65-kDa protein from Xenopus ovary. After in-gel digestion ...

Primary Na+ transport has been essentially attributed to Na+/K+ pump. However, there are functional and biochemical evidences that suggest the existence of a K+-independent, ouabain-insensitive Na+ pump, associated to a Na+-ATPase with similar characteristics, located at basolateral plasma membrane of epithelial cells. Herein, membrane protein complex ...

... proposed to purify the BRCA2 protein and examine its effects on Rad51 using three biochemical assays, including DNA binding, ATPase and DNA ...

... All of the ATP-dependent chromatin-remodeling complexes contain an ATPase subunit that belongs to the SNF2 superfamily of proteins. ...

The subunits from two purified halobacterial membrane enzymes (ATPase and nitrate reductase) behaved