... in compliance with applicable federal and international ... in blood donors throughout the United States has been ... US Department of Labor, Occupational Safety and ...

... Ab EIA Manufacturer: Bio-Rad Laboratories Indication: as an aid in the diagnosis of HIV infection, including in pediatric populations (children as young as two ...

Two murine IgM monoclonal antibodies (MAs) directed against the lipid A portion of bacterial lipopolysaccharide (LPS) were compared in their binding to Re LPS and lipid A and their idiotypic make-up with rabbit anti-idiotypic sera. Horseradish peroxidase (HRPO)-labelled MAs 8-2 and 26-20 bound equally well to Re LPS. The binding of HRPO-labelled MA 8-2 to ...

... Each panel member was tested in triplicate (x3) on 1 run per day for 5 days on 1 lot of the GS HIV Combo Ag/Ab EIA at each site (15 replicates per member per ...

An indirect enzyme immunoassay (EIA) using the purified fraction of surface viral glycoproteins (GP) as an antigen for solid phase sensitization was not shown to be a specific method for the differential detection of influenza A(HS) (HS-Ab) virus antibodies (Abs) due to total conservative epitopes in the structure of GPs of influenza ...

... 3. Avoid microbial contamination of specimens, reagents and water used for washing. ... be shipped ambient, refrigerated (2 to 8�C) on wet ice, or ...

This study investigated the production of polyclonal (pAB) antibodies and the first time production of monoclonal (mAB) antibodies against the mycotoxin alternariol, and their implementation in enzyme immunoassay (EIA) for the rapid determination of alternariol in foods. Both EIAs were highly sensitive, with ...

Monoclonal antibodies (Ab) to human ..cap alpha..-fetoprotein (AFP) were conjugated with diethylenetriaminepentaacetic acid (DTPA) using cyclic DTPA anhydride and the obtained conjugates, DTPA-Ab, were labeled with In-111. The effect of DTPA conjugation on the affinity constant and the maximum binding capacity of ...

... Before making any change affecting the safety or effectiveness of the device, you must submit a PMA supplement or an alternate submission (30-day notice) in ...

This review article describes several applications of the widely used enzyme immunoassay (EIA) procedure. EIA methods have been adapted to solve problems in diagnostic virology where sensitivity, specificity, or practicability is required. Concurrent developments in hybridoma and conjugation methods have increased significantly the use of these assays. A ...

Following a laboratory audit, a significant number of Treponema pallidum particle agglutination assay (TPPA)-negative sera were identified when TPPA was used as a confirmatory assay of syphilis enzyme immunoassay (EIA) screening-reactive sera (SSRS). Sera giving such discrepant results were further characterized to assess their significance. A panel of 226 sera was tested by ...

Following a laboratory audit, a significant number of Treponema pallidum particle agglutination assay (TPPA)-negative sera were identified when TPPA was used as a confirmatory assay of syphilis enzyme immunoassay (EIA) screening-reactive sera (SSRS). Sera giving such discrepant results were further characterized to assess their significance. A panel of 226 sera was tested by ...

The authors suggest a simple sensitive technique for enzyme immunoassay (EIA) of ABH antigens in saliva and semen. A two-staged dot blot solid-phase EIA on nitrocellulose membranes was employed with anti-ABH monoclonal antibodies obtained in immunization of mice with human red cells. 4-chloro-1-naphthol substrate solution was used to visualize the ...

Balb/c mice were immunized with human plasmin-generated fibrinogen degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs) with a strong reactivity with human fibrin and only a weak reactivity with fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with ...

A possibility of simultaneously detecting specific antibodies to HIV-1 and HIV-2 by enzyme immunoassay (EIA) at lower concentrations than those by immunoblotting (IB), and well as an additional possibility of earlier diagnosis of HIV infection, by identifying the HIV-1 antigen p24 lay the foundation of the "DS-EIA-HIV-AB-AG-SPECTRUM" test system made by ...

... a. Laboratories using Quantum II Module A List Number 4045-97, should read the HIVAB HIV-1/HIV-2 (rDNA) EIA using the assay protocol -A ...

... of the Positive and Negative Controls (PN) must ... Assay Inter-Assay Inter-Lot Control N OD ... II. Experimental transmission, putative virus agents and markers, and ...

The advantages of enzyme-immunoassay (EIA) over radioactive assay techniques are mainly convenience in use, in that the labelled immunoreagents are stable for long periods, and the precautions and disposal procedures required for radioisotopes are unneces...

The advantages of enzyme-immunoassay (EIA) over radioactive assay techniques are mainly convenience in use, in that the labelled immunoreagents are stable for long periods, and the precautions and disposal procedures required for radioisotopes are unnecessary. In addition, the us...

Two electrochemical assays for detecting Staphylococcus aureus enterotoxin A and B genes were developed. The assays are based on PCR amplification with biotinylated primers, hybridization to a fluorescein-labeled probe, and detection with horseradish pero...

Immunoassays in pesticide residue analysis significantly profit from the monoclonal antibody (mAb) technology because a sufficient supply of standardized antibodies can be provided. For the production of atrazine-specific mAbs hybridoma cells were produced by fusion of mouse myeloma cells and spleen cells from mice which were immunized with ...

Three systems were tested for the optimization of a heterogeneous noncompetitive enzyme immunoassay (EIA) for the determination of Hg (II). The sensitivity of the nonoptimized Hg-EIA with a detection limit of 2.1 {micro}g/L Hg (II) was improved by an avidin-biotin-complex (ABC) amplification system to a 2-fold lower detection limit (1.1 {micro}g/L Hg ...

An enzyme immunoassay (EIA) for serum immunoglobulin M (IgM) antibodies to rubella virus based on enzyme labeling of viral antigen was developed. The sensitivity of the EIA for the detection of recent rubella virus infection was evaluated by using 115 rubella-IgM-antibody-positive serum specimens, which were confirmed as positive by ...

An enzyme immunoassay (EIA) for serum immunoglobulin M (IgM) antibodies to rubella virus based on enzyme labeling of viral antigen was developed. The sensitivity of the EIA for the detection of recent rubella virus infection was evaluated by using 115 rubella-IgM-antibody-positive serum specimens, which were confirmed as positive by ...

Monoclonal antibody (MoAb) 1D5 with specificity to the estrogen receptor (ER), MoAb 1A6, and a polyclonal antibody (PoAb) (the latter two with specificity to the progesterone receptor [PgR]) were used to stain microwave-pretreated sections of formalin-fixed, paraffin-embedded normal and malignant endometrial tissues (n = 60). The ...

Ga-67 labeled monoclonal IgG or F(ab')/sub 2/ fragments against ..cap alpha..-fetoprotein and ..beta..-subunit of human choriogonadotropin (HCG), were prepared using Deferoxamine (DFO) as a bifunctional chelating agent. DFO, a well-known iron chelating agent, was conjugated with monoclonal antibodies (Ab) by a glutaraldehyde ...

An enzyme immunoassay (EIA) for the capsular polysaccharide of Streptococcus pneumoniae type III was developed and applied to the measurement of this antigen in cerebrospinal fluid (CSF) in an experimental model of pneumococcal meningitis. EIA was performed by a single-antibody sandwich technique in which the globulin fraction of pneumococcal type-specific ...

Two simple and rapid methods were developed to monitor pungency of salsa in production. Capsaicin (C) and dihydrocapsaicin (DHC) were quantitated in 17 commercially available tomato-based salsas by enzyme immunoassay (EIA) and liquid chromatography (LC) with fluorescent detection. Samples were extracted with methanol and the extracts were subjected to solid-phase extraction ...

Monoclonal antibodies (mAbs) are attractive vehicle so far targeting radiation to tumors because of their ability to react specifically with molecular determinants that are either uniquely expressed or over expressed on cancer cells. However, the clinical impact of labeled mAb is hampered by loss of the label from ...

This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The ...

Hepatic uptake of labeled monoclonal antibody (MoAb) remains a persistent problem. Human hepatoma tumors were transplanted into adult rats and successfully imaged following the injection of In-111-antiferritin MoAb. Biodistribution studies revealed that the livers in both tumor and non-tumor bearing rats accumulate and retain ...

The use of bifunctional chelate such as the cyclic anhydride of DTPA for radiolabeling antibodies (Abs) may lead to homopolymerization, and intra- or intermolecular cross-linking, with resulting denaturation and decrease immunoreactivity of Abs. The authors, therefore, investigated the use of heterobifunctional reagents, whereby one group selectively ...

Clostridium difficile is the most common infectious cause of diarrhea in hospitalized patients. The optimal approach for the detection of toxigenic C. difficile remains controversial because no single test is sensitive, specific, and affordable. We have developed a real-time PCR method (direct stool PCR [DPCR]) to detect the tcdB gene encoding toxin B directly from stool specimens and have ...

AA@@@A@AAAAAA@AAA@@ABAAAABAAB@AA@@ABBAAAA@A@AA@@@A@@A@@@AA@@@?@@@@@@??@@? .... A @AAA@AA@@A@@A?A??@@?@@@@@@AB@@@A@@A>?@?@@>>?@A@A@AA@@@??@?????@?@>?@@AB@@? ...

...from such establishment, become void ab initio if the device comprising such shipment...2) of this section shall become void ab initio with respect to the person who...or other media, the equipment, and the testing method or quality controls to be used...

of those that we have tested, we found Hamilton cycles. In the table below the cycles are presented- tions mentioned above are used (labels of graphs are from 1]): 1. 108] (abc)3 = (ab)2c(ba)2c = e, ((ab)2ac)2(ba)2bc(ba)5bc(ac)2(ba)5bc(ba)2bc(ab)2ac(ab)4ac(ab)5ac(ba)2bc ...

Determination of estrogen receptor content in 82 breast cancer specimens with immunocytochemical estrogen receptor assay (ER-EIA) (Abbott) was compared with our routinely used binding assay using /sup 125/I-estradiol as radioligand with Scatchard plot analysis of the binding data. Although the estrogen receptor content measured with the ER-EIA was ...

... Thus for a the absorbency at 330 m# of a 0..5-1% solution of conjugate single preparation of labeled rabbit antibody (TG-Ab-lI) Ps ... tube. ...

Information gathered at time of collection, and in the lab prior to specimen preparation; also label data from the PMAE ornithological collections; received ... ...

... FITC- labeled affinity purified F(ab')2 fragments of rabbit antibodies specific for mouse 6, ,!, or y chains or keyhole limpet hemocyanin (control) or ...

... Survivin-Ab positive cells were detected using an alkaline phosphatase- labeled donkey anti-goat antibody followed by alkaline phosphatase ...

or mononuclear cells obtained by Ficoll-hypaque density gradient centrifugation were labeled with Abs conjugated

The purpose of these studies was to determine if prostate carcinoma (PC) and cutaneous T-cell lymphoma (CTCL) could be detected using the In-lll- MoAbs described in this paper. Murine IgG MoAbs were developed against prostatic acid phosphatase (PAP) and to an antigen present on human T-cells. The MoAbs were labeled ...

Labels based on mesoporous silica nanoparticles (MSN) loaded with mediator thionine (TH), enzyme horseradish peroxidase (HRP) and secondary anti-human IgG antibody (Ab(2)) were developed in order to improve the sensitivity and detection limit of an amperometric immunosensor. The sensitivity of the sandwich-type immunosensor using ...

Adhesion of 3H-labeled Escherichia coli K-12(K88ab) to CD-1 mouse small intestine mucus and brush border preparations, immobilized on polystyrene, was studied. E. coli K12(K88ab) was shown to adhere readily to either crude mucus or brush border preparatio...

experiments, the recycling endosomes were labeled with FITC-F(ab) in DMEM containing 10% serum, chased for 2 FITC-F(ab) fragment (E) was concentrated in the recycling endosomes as was Rh-Tf (F). Bar, 10 m. Cb of Cb and Tf in the recycling endosomes. pHCb Is Lower Than pHTf--The epitope specificity of the F(ab

The specificity of solid-phase radioimmunoassay method for Australian antigen (Au-ag) and anti-Australian antigen (Au-ab) was confirmed. Au-ab was coated quantitatively on the surface of /sup 125/I labeled Au-ag. The /sup 125/I- labeled Au-ag showed positive reaction by using a tube coated with ...

We have previously reported successful imaging of fresh (2-4 hr old) and aged (1-5 days old) canine thrombi with /sup 131/I-labeled intact monoclonal antibody (MAb) specific for fibrin. We now report thrombus imaging with /sup 131/I-labeled F(ab')2 and Fab and /sup 111/In-labeled intact MAb, ...

Quantitative measurements of cultured human and murine thymic, and human thymoma reticuloepithelial cells (REC), immunolabeled by different antibodies (Ab) (TE3, TE4, anti-HTLV p19(p19), lu5, K11 and Aks) and by thymic hormones (thymulin and thymosin alpha 1 (Ta1)) within these cells, were performed using a flow cytometric technique. The anti-keratin polyclonal ...

Med: 20299674] 4. Sourris K.C. Forbes J.M. Interactions between advanced glycation end-products (AGE99mTc-Labeled anti-receptor for advanced glycation endproducts monoclonal antibody F(ab')2 fragments [99mTc-anti-RAGE mF(ab')2] Liang Shan, PhD National Center for Biotechnology Information, NLM, NIH

An immunoprecipitation assay for autoantibodies (Abs) to the human islet cell antigen IA-2 has been developed using 125I-labelled recombinant IA-2 expressed in E. coli. With this assay IA-2 Abs were detected in 103/217 (47%) of IDDM patients of different ages and with different disease duration. IA-2 Ab prevalence ...

The effect of tumor mass and antigenic nature on the biodistribution of 111In- and 125I-labeled monoclonal antibodies (MoAbs) was studied using F(ab')2 fragments of three representative anti-tumor MoAbs and SW1116 human colorectal carcinoma grown in nude mice. The 19-9, F33-104 anti-CEA, and 17-1A ...

Solid-phase immunoglobulin M (IgM) antigen capture enzyme immunoassay (AgCEIA) and antibody capture enzyme immunoassay (AbCEIA) were developed for the diagnosis of BK virus (BKV) infections. Of 37 serum samples from renal allograft recipients, 15 were positive for BKV IgM antibody by either AgCEIA, AbCEIA, or antigen capture radioimmunoassay. ...

Application of hybridoma technology has resulted in the availability of many monoclonal antibodies (MoAbs) which are potentially useful for in vivo tumor localization in cancer diagnosis and therapy. What is now required is a simple and predictive assay which will select those MoAbs with superior properties for clinical trials. The authors have developed a ...

Precision binding of monoclonal antibodies (mAbs) to biological targets, their relative clinical success, and expansion of indications following initial approval, are distinctive clinical features. The relatively high cost of mAbs, together with the absence of a regulatory pathway to generics, stand out as distinctive economic features. Based on both ...

The activity of HCO₃ ^? transporters contributes to the acid-base environment of the nervous system. In the present study, we used in situ hybridization, immunoblotting, immunohistochemistry, and immunogold electron microscopy to localize electrogenic Na/bicarbonate cotransporter NBCe1 splice variants (-A, -B, and -C) in rat brain. The in situ ...

The authors report success in imaging thrombi using labeled monoclonal antibody or antibody fragments. An In-111 labeled antibody fragment appears to be the best imaging agent studied to date.

A previously described, non radioactive method for the measure of in vitro mouse lymphocyte proliferation was applied to human lymphocyte proliferation assays. It involved incorporation into DNA, during cell multiplication, of 5-bromo-2-deoxyuridine (BUdR), a thymidine analogue. BUdR-DNA was then assayed by a sandwich enzyme immunoassay (BUdR-EIA) using an anti-BUdR monoclonal ...

AIMS: To evaluate a digoxigenin-labelled trivalent DNA probe (LT, ST1a, ST1b) for detecting enterotoxigenic Escherichia coli (ETEC), by comparison with a cell culture assay for detecting LT, individual DNA probes for LT, ST1a and ST1b, and an enzyme immunoassay for detecting ST1. METHODS: A 1268 base pair DNA fragment, containing parts of the genes for E coli heat labile ...

Anti-human serum albumin antibody (Ab) was reacted with cyclic DTPA dianhydride (cDTPAA) at various cDTPAA/Ab molar ratios between 1 and 40. Using a carrier In titration method for DTPA and DTPA-antibody conjugate (Ab-DTPA). The authors determined that the above reactions produced between 0.1 and 11 DTPA molecules per either ...

Contains abstracts of survey forms used to collect energy data for the Department of Energy (DOE). Most forms are sponsored by the Energy Information Administration (EIA), but a few non-EIA forms are included.

... BAM R15: Coating Solution for V. vulnificus EIA. January 2001. Bacteriological Analytical Manual. R15 Coating Solution for V. vulnificus EIA. ...

Rauscher leukemia virus glycoprotein gp69/71 is synthesized in virus-infected cells by way of a 90,000 dalton glycoprotein precursor, termed Pr2a+b. This precursor could be labeled with radioactive glucosamine and methionine but not with fucose; whereas gp69/71 could be detected by labeling with radioactive glucosamine, fucose, or a ...

In this study, an IgG2a (KS1/4), a monoclonal antibody (MoAb) specific against a human lung adenocarcinoma (UCLA P-3) was successfully fragmented enzymatically to yield F(ab')/sub 2/ and Fab by using pepsin and papain, respectively. The kinetic of fragmentation of the MoAb was compared to that of human immunoglobulin G (IgG). ...

Conjugation of antibodies (ABs) with bifunction chelates (BFCs) is an effective method to produce immunodiagnostic and immunotherapeutic agents. Most BFCs employ multidentate acetate ligands to chelate metallic radionuclides. The authors have designed a BFC where a cyclam analogue tetradentate macrocyclic amine (MA) is the complexing moiety, MA was linked to the ...

In the search for bifunctional chelating agents (BCA) with better affinity, selectivity and stability as for Tc-99m, synthesis of a novel BCA containing di-thio-semicarbazone as for Tc-99m chelating site has offered interesting characteristics for the labeling of macromolecules. In the present paper, monoclonal IgG (MoAb) against human thyroglobulin was ...

Thyrotropin (TSH) receptor monoclonal antibodies (TSHR mAbs) were obtained from cDNA-immunized NMRI mice. Three mAb immunoglobulin Gs (IgGs) (TSmAbs 1-3) that had distinct V(H )and V(L) region sequences stimulated cyclic adenosine monophosphate (cAMP) production in isolated porcine thyroid cells greater than 10x basal and as little as ...

The aim of this study is to construct a biocompatible coating of a drug-eluting stent through the incorporation of chitosan with monoclonal antibody (mAb) to a platelet glycoprotein (GP) IIIa receptor, by electrostatic layer-by-layer (LBL) adsorption of oppositely charged polyelectrolytes and proteins. The platelet maximum aggregation rate and aggregation inhibition rate tests ...

A comparison of the pharmacokinetics of intact B72.3 (a murine monoclonal antibody specific for human breast and colon carcinoma) with F(ab')2 and Fab fragments labeled with /sup 111/In and /sup 125/I was done in athymic mice bearing target (LS174T) and non-target (HCT-15) tumors. IgG B72.3 labeled with either isotype imaged ...

F(ab')/sub 2/ fragment was chosen for this study because it is divalent for antigen binding and it physiological t1/2 is suitable for /sup 99m/Tc. For a stable label, the authors used (Fab')/sub 2/-DTPA conjugate. A series of control experiments led the authors to a condition resulting in 85% DTPA-/sup 99m/Tc and 15% stable ...

Rapid human immunodeficiency virus (HIV) antibody tests have been adopted into national guidelines for HIV testing in many countries in sub-Saharan Africa. One goal of HIV rapid testing is to minimize the occurrence of indeterminate results. From January 2005 to December 2007, plasma (or serum) samples from pregnant women in Bobo-Dioulasso (Burkina Faso, West Africa) were screened for HIV by using ...

We analyzed the tissue distribution of apelin mRNA in rats by a quantitative reverse transcription-polymerase chain reaction and that of immunoreactive apelin (ir-apelin) by an enzyme immunoassay (EIA) using a monoclonal antibody. The expression levels of apelin mRNA and ir-apelin seemed to be consistent among tissues: they were highly expressed in the lung and mammary gland. ...

Monoclonal antibodies (MoAbs) against carcinoembryonic antigen were successfully radiolabeled with /sup 111/In, and the radiopharmaceutical was characterized in vitro and in normal and tumor-bearing mice. The /sup 111/In-MoAb proved to be stable in vitro and in vivo under normal conditions, although instability could be induced in vitro with large ...

Studies were performed to determine the effect of the radiolabel and circulating carcinoembryonic antigen (CEA) on the pharmacodynamics of monoclonal anti-CEA antibodies (MoAbs). The studies were performed in normal BALB/c mice and in nude mice bearing human colon tumors. Three different tumors were used, each of which produced CEA levels characteristic of that particular ...

Semiconductor quantum dots (QDs) are proved to be unique fluorescent labels providing excellent possibilities for high-throughput detection and diagnostics. To explore in full QDs' advantages in brightness, photostability, large Stokes shift, and tunability by size fluorescence emission, they should be rendered stable in biological fluids and tagged with the target-specific ...

AB005297- 35897_r_at brain-specific angiogenesis inhibitor 1 D80010-38098_at lipin 1 X62025-32204_at

Sheep red blood cells (SRBCs) were labelled with a concanavalin A-luminol-bovine serum albumin conjugate specific for the transmembrane anion transport protein (Band 3) and exposed to 2450 MHz continuous wave microwave radiation at an average specific abs...

... __`a`_aa`___aaaa`__``ababbbaba`_`_`aaaaaa`abba`bbaaaaa_ab`bcbaa`acccccbbbaa` aa`befeeeeffeghffddfeeeeggffeeeedddedddeeeeedcccbbcca`aa`a`aaa``_acdccbbaabb ...

Procedures are described for mapping phonemic strings into a matrix of labeled speech segments that may consist of specific phonemes, groups of phonemes, or spectral classes. A computer program embodying these procedures is described. (Modified author abs...

A model that can be used to show the arrangement of light and heavy chains, disulfide linkages, domains, and subclass variations in antibodies is given. It can be constructed and modified to illustrate Fab, F(ab')2, and Fc fragments, single domain and bifunctional antibodies, and labeling of antibodies. (Author)

...ACT (CONTINUED) COUNTRY OF ORIGIN LABELING FOR FISH AND SHELLFISH General Provisions Definitions... (a)-(b) [Reserved] (c) Farm-raised Fish and Shellfish: From fish or shellfish hatched, raised, harvested,...

The authors have evaluated the potential of in vivo cell surface labeling using radiolabeled monoclonal antibodies (MoAbs) directed against their surface antigens. Two MoAbs, a specific antibody (anti-Thy-1 OX7) and a nonspecific control antibody (anti-CEA) were coupled with DTPA, labeled with /sup 111/In and ...

...false Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental Impact Assessments (EIAs). 26.3...3 Availability of Environmental Impact Assessment Summaries (EIA...

...false Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental Impact Assessments (EIAs). 26.3...3 Availability of Environmental Impact Assessment Summaries (EIA...

The efect of (sup 60)Co (gamma)-rays on the lymphocytes were investigated with T(sub 4), T(sub 8), and HI(sub 4), McAbs. T and B lymphocytes were separated by rosetting procefures. After lymphocytes were labelled by T(sub 4) McAb, T(sub 4)(sup +) and non-...

NKL were immunoprecipitated with a control immunoglobulin (cIg) or affinity-purified anti-DAP10 or a polyclonal NK cell line were labeled with 125 I, lysed in 1% digitonin, and immunoprecipitated with cIg expressing the indicated receptors were stained with cIg, Flag mAb M2, or NKG2D mAb 5C6 [or 1D11 mAb (7

The positron emitting isotope (89)Zr is an ideal radionuclide for use in positron emission tomography (PET) imaging with monoclonal antibodies (mAbs). This article reviews the cyclotron physics of (89)Zr production, and the chemical separation methods for isolating it from yttrium target material. (89)Zr coordination with the bifunctional chelate desferrioxamine B is ...

of RAGE in GenBank � Article on RAGE in OMIM � AGE substance in PubChem � MICAD chapter on 99mTc-anti.M. Interactions between advanced glycation end-products (AGE) and their receptors in the development99mTc-Labeled anti-receptor for advanced glycation endproducts polyclonal antibody F(ab')2

). However, only minor responses were observed in the clinical investigations, and dose escalation studies). In addition, Abs have prolonged blood circulation and present a high radiation dose risk to the bone marrow]-cG250 Ab for its pharmacokinetic behavior and its ability to target tumors in mice and RCC patients (10

investigations and dose escalation studies are ongoing (6). Molecular radiation-absorbed dose to the bone marrow (9). It has been shown that, compared to intact Abs, the smaller pharmacokinetic behavior and tumor-targeting ability in mice and RCC patients (9). However, the investigators

Contains titles and abstracts of periodicals and one time reports produced by EIA from January 2000 through December 2001.

... Resources for You. Premarket Approvals with Supporting Documents. -. Calypte HIV-1 Urine EIA. Applicant: Calypte Biomedical ...

Monoclonal anti-tumor antibodies have great promise for radioimmunodetection and localization of tumors. Fab and F(ab')2 fragments, which lack the Fc fragment of antibody (Ab), are cleared more rapidly from the circulation and may have less nonspecific tissue binding than intact Ab. In radioimaging studies using a murine ...

The transcription of adenovirus type 2 genes proceeds through a broad three-phase program. From 1 to 4 h postinfection six early transcription units (EIa, EIb, EII, EIII, EIV, and the promoter-proximal segment of the late transcription unit) are activated. From 4 to 6 h postinfection transcription of the early genes is depressed. After the onset of viral DNA replication at ...

The transcription of adenovirus type 2 genes proceeds through a broad three-phase program. From 1 to 4 h postinfection six early transcription units (EIa, EIb, EII, EIII, EIV, and the promoter-proximal segment of the late transcription unit) are activated. From 4 to 6 h postinfection transcription of the early genes is depressed. After the onset of viral DNA replication at --6 ...

Delayed pubertal development and low fertility of Bos indicus x Bos taurus crossbred male cattle and domestic buffaloes is hardly understood hence, a sensitive enzymeimmunoassay (EIA) was developed using the second antibody-coating technique and testosterone-3-O-carboxymethyloxime-horseradish peroxidase conjugate as a label for determination of ...

Two monoclonal antibodies (mAb 124D1 and mAb 129C4) raised against the purified alpha subunit were noninhibitory in reactions directed by lacP/sup +/. Reconstitution of dissociated core polymerase in the presence of mAb 129C4 resulted in only a 5% recovery of enzyme activity whereas mAb 124D1 was without ...

High blood background levels of intact radiolabeled monoclonal antibody (MoAb) after intravenous (iv) injection are problematic. The injection of unlabeled polyclonal antimouse Abs following injection with labeled MoAbs produces accelerated MoAb clearance. This study evaluates a Mo antimouse ...

To facilitate a detailed investigation of pinniped lymphoid organs, 30 monoclonal antibodies (mAb) as well as eight polyclonal antibodies (pAb) of different species specificities directed against cell antigens of the hematopoietic system were tested for immunohistochemical cross-reactivity on formalin-fixed, paraffin wax-embedded tissues of harbor seals ...

The authors previously described a simple in vitro assay for evaluating radiolabeled monoclonal antibodies (Abs) as an aid in selecting for clinical trials those with superior tissue binding properties. Tumor or normal tissues as 1 mm cubes are incubated in microtitre-plate wells containing media and indium-111 labeled Abs and, ...

Ammonia borane (AB) is under significant investigation as a possible hydrogen storage material. While many chemical additives have been demonstrated to have a significant positive effect on hydrogen release from ammonia borane, many provide additional complications in the regeneration cycle. Mechanically alloyed hexagonal BN (nano-BN) has been shown to facilitate the release ...

Intact I-125-B72.3 has been previously shown to selectively localize in the LS174T (human colon carcinoma) xenografts in athymic mice. The purpose of these studies was to compare the pharmacokinetics of In-111 and I-125 labeled intact B72.3 F(ab')/sub 2/, and Fab in this model. B72.3 Fab was prepared by standard methods while the ...

BRCAl is an anti-oncogene in women, who are genetically predisposed to breast and ovary cancer. The detection of BRCA1 can offer an opportunity to characterize the function of genetic features in breast and ovarian cancer and to screen breast or ovarian cancer patients. In this study, we designed a new label and fabricated a novel sandwich-type electrochemical immunoassay for ...

A sandwich electrochemical immunosensor for the sensitive determination of alpha fetoprotein (AFP) has been fabricated. Prussian blue modified hydroxyapatite (PB@HAP) was firstly prepared and used as electrochemical label due to the wonderful conductivity and good biocompatibility of HAP. The results proved that the immunosensor fabricated using the label ...

Analysis of nine mouse monoclonal antibodies (mAbs) to the thyrotropin receptor (TSHR) with TSH antagonist activity showed that only one of the mAbs (RSR B2) was an effective antagonist of the human thyroid stimulating autoantibody M22. Crystals of B2 Fab were analyzed by x-ray diffraction and a crystal structure at 3.3 A resolution was obtained. The ...

The purpose of this research was to study the biotin-streptavidin system coupled to an antigen-antibody system to amplify the sensitivity of tumor detection. Human serum albumin (HSA) was used as an antigen and conjugated to Sepharose 4B beads. The beads-HSA were used as a tumor model. Rabbit-anti human serum albumin antibody (Ab) was used as antibody and derivatized with ...

Pulmonary embolism is a common and potentially life-threatening condition, and its correct diagnosis is highly desirable before anticoagulant therapy is initiated. However, the safe and accurate diagnosis of acute pulmonary embolism remains a challenge. Single photon emission computed tomography (SPECT) is a highly sensitive scintigraphic imaging technique. Pulmonary embolism can be detected by ...

A cocktail of three monoclonal F(ab')2 fragments against three distinct epitopes of calcitonin or PDN 21 was labelled with either 111In or 131I. These F(ab')2 fragments, a control 125I-F(ab')2 fragment and 99mTc-pertechnetate were injected into four patients suffering from medullary ...

During one week, beginning 18 days after transplantation, nude mice bearing human colon carcinoma ranging from 115 to 943 mm3 (mean 335 mm3) were treated by repeated intravenous injections of either iodine-131-({sup 131}I) labeled intact antibodies or {sup 131}I-labeled corresponding F(ab')2 fragments of a pool of four ...

An ultrasensitive electrochemical immunosensor based on graphene sheet (GS) has been developed. GS was used to immobilize mediator thionine (TH), horseradish peroxidase (HRP) and secondary anti-prostate-specific antigen (PSA) antibody (Ab2) and the resulting nanostructure (GS-TH-HRP-Ab2) was used as the label for the immunosensor. With ...

In order to verify if the use of nanobeads of poly[phenylacetylene-(co-acrylic acid)] (PPA/AA) in the ELISA test would affect the immune-activity of the antibodies (Ab) and/or the activity of the enzymes used to label the Ab anti-rabbit IGg, in this work we immobilized the horse liver peroxidase labelled ...

Indium-111 (/sup 111/In) labeled monoclonal antibodies (Ab) prepared with a bifunctional chelating agent, diethylenetriaminepentaacetic acid (DTPA), have been used for the radioimmunoimaging of cancer. In the present experiment, using monoclonal Ab against human ..cap alpha..-fetoprotein (AFP) as a model, the authors have studied the ...

. Labeling with 125Iand chloramine T was performed as described (Greenwood et al., 1963). Sperm whale glycopeptides versus isolated HRP-deglycosylated proteins, (2) binding studies of glycoproteins with oxidized-specific carbohydrate recognized by anti-HRP Ab. Purified fasciclin I (70 kDa) was labeled with I251by the chloramine

A-B diblock copolymers of polystyrene-block-poly(methyl methacrylate) were prepared by anionic polymerization and labeled with one naphthaleneeither at the polystyrene block end or at the polystyrene/methyl methacrylate block boundary. B-A-B triblock copo...

The distributions of the two synaptic vesicle proteins p65 [Matthew et al. (1981) J. Cell Biol., 91:257-269] and synapsin I [De Camilli et al. (1983) J. Cell Biol., 96:1337-1354] were compared in macaque monkey retina using pre-embedding immunocytochemistry for both light and electron microscopy. The monoclonal antibody AB-48 against p65 labeled ...

The authors have developed several monoclonal antibodies against human osteogenic sarcoma, one of which; OST7 (IgGl) selectively localized in osteogenic sarcoma xenografts in nude mice. In the present study, F(ab')/sub 2/ fragment was compared with whole IgG and those labeled with In-111 as well as I-131 were used as a radiotracer for the ...

by R. Wes Harrison and Everald Mclennon Conjoint analysis is used to measure the preferences of United States consumers for labeling of biotech foods. The study found that consumers overwhelmingly support mandatory labeling of biotech foods. Results also showed the preferred labeling format is a text disclosure that describes the ...

Common strategy for diagnostics with quantum dots (QDs) uses the specificity of monoclonal antibodies (mAb) for targeting. However QD-mAbs conjugates are not always well suited for this purpose due to their large size. Here, we engineered ultra-small nanoprobes through oriented conjugation of QDs with 13-kDa single-domain antibodies ...

Clinical immunolocalization has been attempted by others with an anti-Thomsen-Friedenreich antigen (TF-Ag) mAb that bound both alpha- and beta-linked TF-Ag. In this report, 124 I-labeled mAb JAA-F11 specific for alpha-linked TF-Ag showed higher tumor specificity in in vivo micro-positron emission tomography (micro-PET) of the mouse ...

There is evidence that the T-cell antigen receptor is immunoglobulin-related and that normal human T cells express surface determinants which cross-react with chicken anti-F(ab')2, anti-alpha and anti-mu antibodies. Surface marker study of six neoplastic human T cell lines suggested that F(ab')2- and mu-cross-reactive determinants may be expressed ...

Thus publication contains information compiled by the Energy information administration (EIA) on the following topics: heating fuel supplies; alternative fuel vehicles; natural gas production; clean air laws and coal transportation; EIA`s world Wide Web Site; EIA`s CD-ROM; Press Releases; Microfiched products; electronic publishing; ...

ABS>Escherichia coli RNA polymerase was prepared, and the synthetic reaction was assayed by the method of Chamberlin and Berg. Phosphorus-32-labeled uridine triphosphate (UTP) was used as the proximally labeled nucleotide. The pyrophosphate-nucleoside triphosphate exchange reaction dependent on crab- daT(deoxyadenylate and ...

ABS>Bean roots were exposed to 140 to 2500 r x rays aerobically during a 16- to 17-min immersion in a solution of thymidine labeled with C/sup 14/ or H/ sup 3/. There was no evidence of increase in the rate of DNA synthesis due to irradiation, nor in the number of cells which became labeled. On the contrary, radiation ...

CDC recommends syphilis serologic screening with a nontreponemal test, such as the rapid plasma reagin (RPR) or Venereal Disease Research Laboratory (VDRL) test, to identify persons with possible untreated infection; this screening is followed by confirmation using one of several treponemal tests. Recently, the availability of automatable treponemal enzyme and chemiluminescence immunoassays ...

A delta virus (DV) infection was experimentally transmitted to a hepatitis B chronic carrier chimpanzee. The infection was monitored by the examination of liver biopsy materials with the use of FITC-labeled human anti-delta IgG or by an indirect immunoperoxidase staining technique and by measurement of the serum alanine aminotransferase (ALT) activity. Three different antibody ...