Primosome protein PriB is a single-stranded DNA-binding protein that serves as an accessory factor for PriA helicase-catalyzed origin-independent reinitiation of DNA replication in bacteria. A recent report describes the identification of a novel PriB protein in Klebsiella pneumoniae that is significantly shorter than most sequenced ...
PubMed Central
ABSTRACT: BACKGROUND: Bacterial DNA replication restart pathways facilitate reinitiation of DNA replication following disruptive encounters of a replisome with DNA damage, thereby allowing complete and faithful duplication of the genome. In Neisseria gonorrhoeae, the primosome proteins that catalyze DNA replication restart differ from the well-studied primosome proteins of E. coli with respect to ...
PubMed
BackgroundBacterial DNA replication restart pathways facilitate reinitiation of DNA replication following disruptive encounters of a replisome with DNA damage, thereby allowing complete and faithful duplication of the genome. In Neisseria gonorrhoeae, the primosome proteins that catalyze DNA replication restart differ from the well-studied primosome proteins of E. coli with respect to the number ...
Both C/sup 14/- and /sup 13/C-phosphoribosyl 1-pyrophosphate molecules, labeled at the C'-1 position, were synthesized biochemically with bacterial ribokinase and PRibPP synthetase activities. A flow dialysis experiment characterized the dissociation constant (33 ..mu..M) for the OPRTase-C/sup 14/PRibPP-Mg(II) complexation and defined 2 ...
Energy Citations Database
Quantitative analysis of the interactions of the Escherichia coli primosomal PriB protein with a single-stranded DNA was done using quantitative fluorescence titration, photocrosslinking, and analytical ultracentrifugation techniques. Stoichiometry studies were done with a series of etheno-derivatives of single-stranded (ss) DNA oligomers. Interactions with the unmodified ...
This thesis describes the elucidation of Mn(II)-PRibPP conformations free in solution as well as bound to OPRTase and HGPRTase from yeast using /sup 1/H and /sup 31/P magnetic relaxation rate measurements. The eight PRibPP metal-to-nucleus distances determined were used to resolve the conformation of the binary Mn(II)-PRibPP, ternary ...
Translated from Prib. Tekn. Eksp.; 16: No. 4, 67-68(1973). A procedure is described which allows estimation of the linearity of the working characteristic of ionization chambers according to the slope of the volt-ampere characteristic. (auth)
Translated from Prib. Tekh. Eksp.; 16: No. 3, 38-41(1973). The instrument is used to investigate radiative electron emission from radioactive substances. The instrument energy halfwidth of the lines is 0.08%. (auth)
Translated from Prib. Tekh. Eksp.; 16: No. 3, 65-66(1973). A new elementary-particle detector is described--- a dischargediffusion chamber; one of the first photographs of the track of a cosmic-radiation particle in a chamber having an argon filler is shown. (auth)
Structures of free, substrate-bound and product-bound forms of Escherichia coli xanthine-guanine phosphoribosyltransferase (XGPRT) have been determined by X-ray crystallography. These are compared with the previously determined structure of magnesium and sulphate-bound XPRT. The structure of free XGPRT at 2.25 A resolution confirms the flexibility of residues in and around a mobile loop identified ...
Daly,R. Edwards,K.D. O'Neill,J.S. Aitken,S. Millar,A.J. ...
E-print Network
Reactivation of repaired DNA replication forks is essential for complete duplication of bacterial genomes. However, not all bacteria encode homologs of the well-studied Escherichia coli DNA replication restart primosome proteins, suggesting that there might be distinct mechanistic differences among DNA replication restart pathways in diverse bacteria. Since reactivation of repaired DNA replication ...
Translated from Prib. Tekh. Eksp.; 16: No. 4, 25-28(1973). A transistorized device is described which allows automatic tuning of the resonant frequency of the dee tuned circuit and elimination of the asymmetry of the accelerating voltage of a cyclotron. The device is used in the operation of a cyclotron as a pulsed source for neutron spectrometry by the ...
Translated from Prib. Tekn. Eksp.; 16: No. 4, 75-77(1973). A method is considered for determining the energy equivalent of the noise of semiconductor nuclear-radiation detectors which is based on calibrating a simplified measurement device consisting solely of a spectrometric preamplifier and a vacuum- tube voltmeter. The method allows a considerable increase in the ...
Translated from Prib. Tekh. Eksp.; 16: No. 3, 31-34(1973). The circuit of an instrument for measuring the position and shape of a beam on an investigated target is described. The circuit is designed for operation with a beam-position pickup of the monitor or ionization type. The accuracy with which the beam position is measured is at worst plus or minus 0.5 mm in ...
and proteomic data through fractal representation, with an example of comparing oligomer frequencies between the solution of compressing information about oligomer frequencies in long sequences into small, coloured here is to map all oligomers of fixed size of N bases contained in the genome to a 2D space with 2N x2N
Translated from Prib. Tekh. Eksp.; 16: No. 4, 228-230(1973). The results of investigations of an accelerator-tube element having electrodes that are irradiated by helium ions are described. It is shown that the dielectric strength of such an element (120 kV/cm) is double that of the dielectric strength of an element having nonirradiated electrodes (60 kV/cm). ...
... Title : ABS in the Auto Industry,. ... The history of ABS in the automotive industry and the properties which make it useful in this area are discussed. ...
DTIC Science & Technology
... Aviano AB Italy Masirah AB Oman Manas Kyrgyzstan Moron AB Spain Seeb lAP Oman Masirah AB Oman Balikesir Turkey ...
... Title : Modification of PVC Compounds with ABS,. Corporate Author : ... Some flexible ABS/PVC compounds contain as much ABS as they do PVC. ...
Translated from Prib. Tekh. Eksp.; 16: No. 3, 66-69(1973). Equipment for control and monitoring is described which is designed for the connection of 1 to 6 RFK-5 streak cameras. Principal attention is devoted to the circuit for automatic monitoring of the film transport and synchronous operation of several cameras. The installation is implemented using cells of ...
A direct quantitative analysis of the initial steps in primosome assembly, involving PriA and PriB proteins and the minimal primosome assembly site (PAS) of phage ?X174, has been performed using fluorescence intensity, fluorescence anisotropy titration, and fluorescence resonance energy transfer techniques. We show that two PriA molecules bind to the PAS at both strong and ...
