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1
Helium-Neon Laser Preirradiation Induces Protection against UVC Radiation in Wild-Type E. coli Strain K12AB1157

... mW as measured by an Ophir 10A-P power meter. Growth of Bacterial Culture and Irradiation with Helium- ... ...

NBII National Biological Information Infrastructure

2
Rifampin-Stimulated Uv Resistance of Phage lambda on Escherichia Coli K12.
1983-01-01

The plaque survival of uv-irradiated phage lambda on excision-proficient E.coli strain AB1157 is greater if the host cells are exposed to rifampin for 10 minutes prior to infection. This repair is accompanied by little or no clear-plaque mutagenesis. Host...

National Technical Information Service (NTIS)

3
Interactions between uv radiation of different energies in the inactivation of bacteria.
1978-10-01

A strong lethal interaction was observed between various monochromatic wavelengths (254, 334, 365, and 405 nm) in the repair-proficient E. coli K-12 strain AB 1157, except in the case of preexposure to 405-nm radiation which resulted in a protection against the inactivation resulting from subsequent exposure to 365-or 254-nm ...

PubMed Central

4
RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K- 12
1964-02-01

The fate of thymine dimers in DNA during incubation after uv light irradiation was studied in two strains of E. coli K-12. One was a multiply auxotrophic strain, AB1157, and the other was a uv-sensitive mutant, AB1886, derived from it. Strain AB1886 is unable to reactivate uv-irradiated ...

Energy Citations Database

5
Irradiance dependence of the He-Ne laser-induced protection against UVC radiation in E. coli strains.
2003-03-01

He-Ne laser pre-irradiation-induced protection against UVC damage was investigated in wild-type E. coli K12 strain AB1157 and its isogenic DNA repair mutant strains. At a dose of 7 kJ/m(2), pre-irradiation was observed to induce protection in recA proficient strains ...

PubMed

6
uv-induced alleviation of K-specific restriction of bacteriophage lambda. [Escherichia coli
1977-03-01

A partial release of K-specific restriction of phage lambda grown in Escherichia coli C was observed when E. coli K strains AB1157 (having wild-type repair of uv-produced DNA damage) and AB1886 (uvrA) were irradiated with uv light before infection. The effect occurred in AB1886 at lower uv fluences than it did in AB1157. Little or no ...

Energy Citations Database

7
Cloning of Bacteroides fragilis plasmid genes affecting metronidazole resistance and ultraviolet survival in Escherichia coli
1990-03-01

Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and C mutant strains ...

Energy Citations Database

8
A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli
1989-10-01

The plasmid pMucAMucB, constructed from the Haemophilus influenzae vector pDM2, and a similar plasmid, constructed from pBR322, increased the survival after UV irradiation of Escherichia coli AB1157 with the umu-36 mutation and also caused UV-induced mutation in the E. coli strain. In H. influenzae, pMucAMucB caused a small but ...

Energy Citations Database

9
Ultraviolet (193, 216 and 254 nm) photoinactivation of Escherichia coli strains with different repair deficiencies
1995-03-01

Escherichia coli K12 bacteria strains AB1157 (repair-deficient wild-type, uvrA{sup +} recA{sup +}), AB1886 (uvrA{sup {minus}}), AB2463 (recA{sup {minus}}) and AB2480 (uvrA{sup {minus}} recA{sup {minus}}) were exposed to 254 nm germicidal UV and 216 or 193 nm laser radiation. The mean lethal incident dose (D{sub 37}) for AB1157 does not ...

Energy Citations Database

10
Radiation sensitivity of N. pharaonis in comparison with E. coli K12 strains.
2002-06-01

To investigate the radiation sensitivity of the natronobacterium Natronomonas pharaonis in comparison with Escherichia coli strains (N. pharaonis DSM 2160T, E. coli strains AB1157 and K12 lambda s) were exposed to gamma-radiation (60Co-gamma-source, 100 Gy min-1) in the presence of oxygen (air) and under strongly ...

PubMed

11
Evidence for particle-induced horizontal gene transfer and serial transduction between bacteria.
2011-03-23

Incubation of the amino acid-deficient strain Escherichia coli AB1157 with particles harvested from an oligotrophic environment revealed evidence of horizontal gene transfer (HGT) with restoration of all deficiencies in revertant cells with frequencies up to 1.94 � 10(-5). None of the markers were preferentially transferred, ...

PubMed

12
Oxygen dependence of sensitization to 254-nm radiation by prior exposure to 365-nm radiation in strains of Escherichia coli K12 differing in DNA repair capability
1979-10-01

Near-ultraviolet radiaton (320 to 400 nm) can produce both lethal and mutagenic effects in bacteria and other biological systems. It has been proposed that biological effects associated with DNA lesions produced by far-uv, near-uv, and x radiation are enhanced by near-uv damage to the DNA repair systems. The relationships between oxygen dependence of enhancement of sensitivity to 254-nm radiation ...

Energy Citations Database

13
[Role of constitutive and inducible repair in radiation resistance of Escherichia coli].
2011-07-01

Radiation resistance of Escherichia coil cells depends on how efficiently DNA is recovered after damage, which is determined by the function of constitutive and inducible repair branches. The effects of additional mutations of the key genes of constitutive and inducible repair (recA, lexA, recB, polA, lig, gyr, recE, recO, recR, recJ, recQ, uvrD, helD, recN, and ruv) on radiation resistance were ...

PubMed

14
Rifampin-stimulated uv resistance of phage lambda on Escherichia coli K12
1983-01-01

The plaque survival of uv-irradiated phage lambda on excision-proficient E.coli strain AB1157 is greater if the host cells are exposed to rifampin for 10 minutes prior to infection. This repair is accompanied by little or no clear-plaque mutagenesis. Host cells uv-irradiated and incubated for 30 minutes in growth medium prior to ...

Energy Citations Database

15
Physical characterisation of the "Rac prophage" in E. coli K12.
1979-09-01

We confirm the hypothesis of Low (1973) that many E. coli K12 strains contain a prophage (the Rac prophage) located a few minutes clockwise of the trp operon on the genetic map. We have used restriction endonucleases and 32P-labelled probes to construct a physical map of this prophage. Some E. coli K12 strains, including ...

PubMed

16
Mutations in Escherichia coli altering an apurinic endonuclease, endonuclease II, and exonuclease III and their effect on in vivo sensitivity to methylmethanesulfonate
1977-12-13

The levels of endonuclease II, an apurinic endonuclease, and exonuclease III in the parent strain (AB 1157) of Escherichia coli and in various mutants were determined by chromatography on DEAE-cellulose. AB 3027 and NH 5016 lacked endonuclease II and exonuclease III. BW 2001 lacked the apurinic endonuclease and exonuclease III while BW ...

Energy Citations Database

17
Effects of a chayotte (Sechium edule) extract (macerated) on the biochemistry of blood of Wistar rats and on the action against the stannous chloride effect.
2007-03-01

The use of natural products as medicines has been growing in the entire world. There are concerns that these products may contain potentially toxic ingredients and contaminants such as heavy metals. The labeling of blood constituents with technetium-99m has been influenced by the presence of natural extracts. We evaluated the influence of a chayotte (Sechium edule) extract (100% v/v macerated) on ...

PubMed

18
The oxygen effect: variation of the K-value and lifetimes of O2-dependent damage in some glutathione-deficient mutants of Escherichia coli.
1991-05-01

Radiosensitization by various concentrations of O2 has been studied in an Escherichia coli K-12 wild-type strain and some derived glutathione (GSH)-deficient mutants using 60Co gamma-irradiation. The maximum oxygen enhancement ratio (OER) and the K-value, the O2 concentration that produced half the maximum O2 effect, were found to depend on the GSH biosynthetic capacity of the ...

PubMed

19
Synergism between 365- and 254-nm radiations for inactivation of Escherichia coli
1978-05-01

The lethal interaction between radiations at 254 and 365 nm was studied in strains of Escherichia coli K12 that differ in repair capability. Increased sensitivity to 254-nm radiation was produced by a prior exposure to large fluences of 365-nm radiation for both stationary-phase and exponential-phase cells of strains K12 ...

Energy Citations Database

20
Protective effect of an aqueous extract of Harpagophytum procumbens upon Escherichia coli strains submitted to the lethal action of stannous chloride.
2007-04-15

Regardless of its lethal effects upon Escherichia coli (E. coli) cultures trough the production of free radicals (FR), stannous chloride (SnCl2) remains to be the most used reducing agent on the production of technetium-99m radiopharmaceuticals, to obtain images on nuclear medicine. Moreover, authors have reported that vegetal extracts are able to protect Escherichia coli cultures against the ...

PubMed

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21
Bacterial glutathione: a sacrificial defense against chlorine compounds.
1996-04-01

Aerobic organisms possess a number of often overlapping and well-characterized defenses against common oxidants such as superoxide and hydrogen peroxide. However, much less is known of mechanisms of defense against halogens such as chlorine compounds. Although chlorine-based oxidants may oxidize a number of cellular components, sulfhydrl groups are particularly reactive. We have, therefore, ...

PubMed Central

22
Bacterial glutathione: a sacrificial defense against chlorine compounds.
1996-04-01

Aerobic organisms possess a number of often overlapping and well-characterized defenses against common oxidants such as superoxide and hydrogen peroxide. However, much less is known of mechanisms of defense against halogens such as chlorine compounds. Although chlorine-based oxidants may oxidize a number of cellular components, sulfhydrl groups are particularly reactive. We have, therefore, ...

PubMed

23
Effect of the Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts against the stannous chloride oxidative damage in Escherichia coli.
2001-09-20

Stannous ion has been used in different sectors of human interest, such as in food industry and in health sciences. Much is known about stannous chloride (SnCl(2)) toxicity, although, there is no general agreement regarding its genotoxicity. Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts have been used in popular medicine. We evaluated the influence of these crude ...

PubMed

24
In vivo and in vitro cloning and phenotype characterization of tellurite resistance determinant conferred by plasmid pTE53 of a clinical isolate of Escherichia coli.
1998-01-01

A determinant encoding resistance against potassium tellurite (Te(r)) was discovered in a clinical isolate of Escherichia coli strain KL53. The strain formed typical black colonies on solid LB medium with tellurite. The determinant was located on a large conjugative plasmid designated pTE53. Electron-dense particles were observed in cells harboring pTE53 ...

PubMed

25
Biological effects of rutin on the survival of Escherichia coli AB1157 and on the electrophoretic mobility of plasmid PUC 9.1 DNA.
2002-07-01

The use of natural products as medicines is growing in the world. The rutin, a compound isolated from Ruta graveolens, is a flavonoid, which has been suggested to have antioxidant properties and to reduce the triacylglycerol levels. In this study, plasmid desoxyribonucleic acid (DNA) was exposed to rutin (0.33, 10, 20, 30 microg/ml) in presence of stannous chloride (SnCl2), a reducing agent widely ...

PubMed

26
Sinergismo entre radiacao ultravioleta e redutona em Escherichia coli UVR: analise quantitativa das lesoes produzidas. (Synergism between ultraviolet radiation and reductone in Escherichia coli UVR: quantitative analysis of produced injuries).
1980-01-01

The relationship between cell viability and DNA-single and double-strand breaks induced by UV-reductone treatment of E.coli AB1157 and AB1886 cells was studied. A molecular and quantitative analysis was done by sedimentation in sucrose gradients. Bacterio...

National Technical Information Service (NTIS)

27
Influence of Pre-Radiation Cultivation Conditions on Induction of Mutation in gamma -Irradiated Bacteriae Escherichia Coli.
1988-01-01

Influence of pre-radiation cultivation conditions on survival and mutation frequency of gamma -irradiated bacteriae E. coli AB 1157 and Hfr H has been studied. The radiation sensitivity of cells and mutagenesis are shown to depend on the cultivation mediu...

National Technical Information Service (NTIS)

28
Correlation of Radiation Sensitivity and Nitrofurantoin Sensitivity of Escherichia Coli K-12.
1981-01-01

The Uvr- and rec-mutants of E.coli K-12 have been tested with a view to their radiation- and nitrofuration sensitivity. The tests showed that all mutants tested were more radiation- and NF-sensitive than the wild type AB 1157. When the NF-sensitivity had ...

National Technical Information Service (NTIS)

29
Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride.
2009-08-20

Stannous chloride (SnC12) is used in nuclear medicine as a reducing agent to obtain technetium-99m-radiopharmaceuticals. It have been reported that natural products might reduce the genotoxic and cytotoxic effects related to SnC12. This work evaluated the biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli (E. coli) ...

PubMed

30
Comparative evaluation of different pairs of DNA repair-deficient and DNA repair-proficient bacterial tester strains for rapid detection of chemical mutagens and carcinogens.
1982-02-01

The aim of the present study was to evaluate the usefulness of different pairs of DNA repair-deficient and DNA repair-proficient bacterial tester strains in a mutagenicity/carcinogenicity screen, possibly as complements to the Ames test. 70 carcinogenic and non-carcinogenic compounds, representing a variety of chemical structures, were tested for their DNA-damaging effects, ...

PubMed

31
NON-DESTRUCTIVE METHOD FOR TESTING AND STRAIN ...
1985-11-01

... Title : NON-DESTRUCTIVE METHOD FOR TESTING AND STRAIN HARDENING OF BEARING BUSHES. Corporate Author : ...

DTIC Science & Technology

32
The effect of homocysteine thiolactone and its ?-alkylated derivative on the survival of irradiated E. coli AB1157
1994-11-01

The radiation induced decomposition of homocysteine thiolactone (HCTL) and ?-methyl-homocysteine thiolactone (?-MHCTL) was studied in aqueous solution (pH = 5.4) as a function of dose (up to 1.55 kGy) in the absence and presence of oxygen as well as in solutions saturated with N2O. The strongest radiolysis of both substances was observed in oxygenated solution, because of the peroxide transient ...

NASA Astrophysics Data System (ADS)

33
Vitamin C acts as radiation-protecting agent
1998-01-01

It is well known that vitamin C (L-ascorbic acid) is a very efficient, water soluble antioxidant. Its multifunctional biological and biochemical activities are rather well established in the last few decades (e.g. Sies and Stahl, 1995; Meydani et al., 1995; NRC, 1989. In the present letter we are reporting briefly the pronounced radiation-protecting properties of ascorbate (AH-) observed on ...

NASA Astrophysics Data System (ADS)

34
Radiosensitization by non-nitro compounds
1982-03-01

The effects of 23 non-vitro compounds on the radiosensitivity of hypoxic Chinese hamster V79-379A or E. coli AB 1157 cells in vitro are outlined. Imidazole derivatives substituted with several alternative electron-withdrawing groups are described; the dicyanovinyl function conferred considerable radiosensitizing activity. 2,4,5-Tribromoimidazole and ...

Energy Citations Database

35
New Crankshaft Bearing Test Rig.
1999-01-01

There are numerous different types of bearings operating under demanding conditions in the internal combustion engines. The crankshaft or main bearings belong to the group of most strained bearings. While the new high performance engines are developed, th...

National Technical Information Service (NTIS)

36
Hardness and Bearing Strength of Lunar Surface - NASA - NSSDC ...

Jul 22, 2011 ... The objective of this experiment was to determine the hardness and bearing strength of the lunar surface by the use of strain gages, ...

NASA Website

37
An Air Bearing Balance with 1-DOF Spin Capability
2011-05-14

... such as strain gage or LVDT balances, where increased ... The 1-DOF air bearing balance was designed to fulfill ... to a high pressure is balanced by an ...

DTIC Science & Technology

38
Cyclic Plastic Strain Energy as a Damage Criterion and ...

... Title : Cyclic Plastic Strain Energy as a Damage Criterion and Environmental Effect in Nb-Bearing High Strength, Low Alloy Steel. ...

DTIC Science & Technology

39
MEMS For Rolling-Element Bearings.
2010-01-01

Improvement of the operating characteristics of rolling-element bearings was sought through development and implementation of MEMS (Micro Electro-Mechanical System) based strain sensors. The strain sensors developed in this program employ a piezoresistive...

National Technical Information Service (NTIS)

40
A mobile-bearing knee prosthesis can reduce strain at the proximal tibia.
2006-06-01

Mobile and fixed-bearing knee prostheses are likely to generate distinct strain gradients in the proximal tibia. The resulting strain distribution in the proximal tibia governs bone remodeling and affects implant integration and stability. We determined the effects of fixed and mobile-bearing total knee prostheses ...

PubMed

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41
Service Life Determination for the UH-60A (UTTAS) Helicopter Elastomeric Bearings.
1980-01-01

A method for predicting the endurance life of the elastomeric bearings on the UTTAS helicopter rotor is presented. The critical layers, based on elastomer strain, are determined analytically for these two bearings. Representative standard laboratory test ...

National Technical Information Service (NTIS)

42
STRUCTURAL STUDIES OF BEARING STEEL ...
1966-04-29

... FATIGUE(MECHANICS), STRAIN(MECHANICS), SHEAR STRESSES, PLASTIC PROPERTIES, MICROSTRUCTURE, ELECTRON MICROSCOPY. ...

DTIC Science & Technology

43
Photoreactivation of Ultraviolet-Irradiated, Plasmid-Bearing ...
1985-12-19

... STRAINS(BIOLOGY), KINETICS, MUTAGENS, BACTERIAL DISEASES, TOXINS AND ANTITOXINS, DIMERS, INACTIVATION, PYRIMIDINES. ...

DTIC Science & Technology

44
Geographical Distribution of Genotypic and Phenotypic ...
2009-01-09

... Abstract : The geographical distribution of Bacillus anthracis strains and isolates bearing some of the same genetic markers as the Amerithrax ...

DTIC Science & Technology

45
Coastal Surveillance
1980-04-01

... The space between the 6" tube and the expanded ... Electrical connection from the hydrophone is by means ... link as well as a compliant strain bearing ...

DTIC Science & Technology

46
Oxygen sensitivity of an Escherichia coli mutant.
1992-04-01

Genetic evidence indicates that Oxys-6, an oxygen-sensitive mutant of Escherichia coli AB1157, is defective in the region of the hemB locus. Oxys-6 is capable of growth under aerobic conditions only if cultures are initiated at low-inoculum levels. Aerobic liquid cultures are limited to a cell density of 10(7) cells per ml by the accumulation of a ...

PubMed Central

47
Oxygen sensitivity of an Escherichia coli mutant.
1992-04-01

Genetic evidence indicates that Oxys-6, an oxygen-sensitive mutant of Escherichia coli AB1157, is defective in the region of the hemB locus. Oxys-6 is capable of growth under aerobic conditions only if cultures are initiated at low-inoculum levels. Aerobic liquid cultures are limited to a cell density of 10(7) cells per ml by the accumulation of a ...

PubMed

48
Cytostatica efficiency enhancement by vitamins C, E and ?-carotene under irradiation. State of the art
2001-01-01

A review is presented on the enhancement of MMC (mitomycin C) efficiency by the vitamins C, E and ?-carotene using E. coil bacteria (AB 1157) and leukemia cells (HL 60) as a model for experiments in vitro. New and previously published spectroscopic and kinetic data of transients obtained by pulse radiolysis of MMC and the above mentioned vitamins were also ...

NASA Astrophysics Data System (ADS)

49
Cytostatic action of aspirin and its effect on mitomycin C activity. A study in vitro under irradiation
2001-04-01

Experiments in vitro using E. coli bacteria (AB 1157) proved that aspirin possesses a cytostatic ability under various experimental condition (pH=7.4) in airfree, aerated as well as in media containing N2O (converting eaq- into OH- radicals). In the last case the highest effect of aspirin was observed. The combination of aspirin with the well-known ...

NASA Astrophysics Data System (ADS)

50
Thermo-resistance Acquisition of A Mesophilic Bacterium with The Aid of Vector Particles Originating from Thermophiles
2004-12-01

The present study was aimed to examine whether virus-like particles (VLPs) would be able to transfer and express the thermo-resistance gene of thermophilic microbes in the mesophilic auxotrophic Escherichia coli AB1157 mutant. A hyper-thermophilic archaea, Thermococcus kodakaraensis B41, that was isolated from the Suiyo Seamount APSK06 boring core, ...

NASA Astrophysics Data System (ADS)

51
Structural Studies of Bearing Steel Undergoing Cyclic Stressing.
1966-01-01

Accumulations of plastic strain, visible in the light microscope as white etching deformation bands, are shown to develop in bearing steels undergoing cyclic stressing. Under the electron transmission microscope the deformation bands appear as cell like s...

National Technical Information Service (NTIS)

52
EVALUATION OF TENSILE DATA FOR BRITTLE MATERIALS OBTAINED WITH GAS BEARING CONCENTRICITY
1963-05-01

Using gas bearings to provide uniaxial loading, the ultimate tensile strengths of an alumina and a graphite were investigated statistically at 70, 1000, and 1600 deg F and the strengths correlated with volume, surface finish, and strain rate. (auth)

Energy Citations Database

53
Copyright � 2006 by ASME1 Fig. 1: Coronal MRI images of the buttock from a healthy 29

-weight-bearing MRI Weight-bearing MRI During sitting INTRODUCTION A common but serious malady afflicting wheelchair. It will be extremely useful to extend the present study and test the effect of special mattress and cushions on strain

E-print Network

54
Behavior of Masonry Plates in Bridge Bearing Systems: Experimental Study.
1990-01-01

An experimental investigation of the behavior of highway bridge masonry bearing plates was conducted and is discussed in the report. Two 12 inch cubic concrete blocks and two 15 inch cubic concrete blocks were instrumented with internal strain gages. Stee...

National Technical Information Service (NTIS)

55
Digital and analog readout systems for fiber-optic strain sensors as applied to the monitoring of roller element bearing systems.
2003-11-01

A rotating machinery test rig was instrumented with fiber Fabry-Perot interferometer strain sensors for condition monitoring of rolling element bearings. Strain variations produced by ball passes were observed and analyzed in the time and frequency domain. Wavelength division multiplexing was utilized to simultaneously monitor the ...

PubMed

56
Studies on Kinetics of Strain Ageing in Nb, Ti, V-Bearing HSLA ...

... Corporate Author : SHEFFIELD UNIV (UNITED KINGDOM). Personal Author(s) : Panda, Ashok K. ; Ganguly, Ratan I. ; Misra, Somnath. ...

DTIC Science & Technology

57
RESPONSE OF DEEP REINFORCED CONCRETE SLABS
1965-02-01

... The bearing stress under the weight was less ... etc., could easily have caused higher stresses in the ... appears to reduce the compressive strains in the ...

DTIC Science & Technology

58
Effect of Advanced Component Technology on Helicopter ...

... of the OH-58C helicopter 236-kW (317 ... Transmission; Gears; Bearings; Transmission testing; Efficiency ... Strain; Deflections; Helicopters; High-contact ...

DTIC Science & Technology

59
Stresses in a Webbed Ball.
1975-01-01

An experimental stress analysis was undertaken to evaluate stresses within cylindrically hollow reinforced webbed bearing balls proportioned for a 50 percent mass reduction. Strain gage rosettes were used to determine principal strains and stresses in the...

National Technical Information Service (NTIS)

60
Role of Ring Strain and Steric Hindrance in a New Method for Synthesis of Macrocyclic and High Polymeric Phosphazenes,
1989-01-01

Ring strain induces the ring-opening polymerization of phosphazene trimers that bear no halogen substituents. This is of considerable importance because it had previously been assumed that side group halogen atoms were a prerequisite for access to the rin...

National Technical Information Service (NTIS)

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61
Precipitation of Nb(CN) during High Strain Rate Compression ...
1984-01-01

... Accession Number : ADD129166. Title : Precipitation of Nb(CN) during High Strain Rate Compression Testing of a 0.07 Pct Nb-Bearing Austenite,. ...

DTIC Science & Technology

62
Plasmid-Determined Ability of a 'Salmonella Tennessee' Strain to Ferment Lactose and Sucrose.
1975-01-01

The ability of a Salmonella Tennessee strain to ferment both lactose and sucrose was attributed to a conjugally transmissible plasmid, deoxyribonucleic acid molecular weight 164 megadaltons, bearing the genetic determinants of both fermentation characters...

National Technical Information Service (NTIS)

63
Experimental Evaluation of Stresses in Cylindrically Hollow (Drilled) Balls.
1974-01-01

An experimental stress analysis was undertaken to evaluate stresses within cylindrically hollow (drilled) bearing balls proportioned for 40, 50, and 60 percent mass reductions. Strain gage rosettes were used to determine principal strains and stresses in ...

National Technical Information Service (NTIS)

64
Characterization of an Hfr Strain of Shigella Flexneri.
1964-01-01

A high frequency of recombination donor (Hfr) Shigella flexneri, strain 69, was obtained by terminal marker selection in a cross between Hfr Escherichia and S. flexneri. The chromosome of this Hfr Shigella bears gross homology to the E. coli chromosome: i...

National Technical Information Service (NTIS)

65
Stress-strain and limiting state of spoked flywheels formed from composite materials. report 1. computational relationships
1986-04-01

The authors compute the stress-strain state of a flywheel with consideration given to the joint deformation of the rim and spokes. The bench tests of the flywheel model presented suggest that its ultimate bearing capacity is exhausted at a rotation frequency of 50,000 rpm. This can be considered to be in good agreement with the results of computation, ...

Energy Citations Database

66
Direct detection and isolation of plasmid-bearing virulent serotypes of Yersinia enterocolitica from various foods.
1997-12-01

A procedure was developed for direct detection, isolation, and maintenance of plasmid-bearing virulent serotypes of Yersinia enterocolitica from different food sources. Plasmid-bearing virulent strains of Y. enterocolitica representing five serotypes were simultaneously detected and isolated from enriched swab samples of artificially ...

PubMed Central

67
Fiber optic strain gauge calibration and dynamic flexibility transfer function identification in magnetic bearings
2003-05-01

Historical attempts to measure forces in magnetic bearings have been unsuccessful as a result of relatively high uncertainties. Recent advances in the strain-gauge technology have provided a new method for measuring magnetic bearing forces. Fiber optic strain gauges are roughly 100 times more sensitive than ...

E-print Network

68
Reactivation of Escherichia coli cells, inactivated by ultraviolet rays, with cell extracts of propionic acid bacteria: Fractionation of the extract
1995-01-01

Separation of Propionibacterium shermanii extract into fractions and testing them for their reactivating effect on UV-inactivated Escherichia coli AB-1157 cells showed that the activity was associated with the fraction of soluble proteins. The activity was not demonstrated in the fractions of RNA, DNA, ribosomes, or cell walls. Fractional salting out and ...

Energy Citations Database

69
LET-dependent radiosensitivity of Escherichia coli K-12 rec and uvr mutants. [Alpha particles
1977-07-01

Escherichia coli K-12 AB1157 (rec/sup +/uvr/sup +/), AB2500 (uvrA), AB2470 (recB), and JC1553 (recA), which have different DNA repair capacity, were bombarded with ..cap alpha.. particles, C ions, and N ions having various beam energies in order to determine the LET-dependent radiosensitivity. Radiosensitivity of the uvrA mutant was almost the same as that ...

Energy Citations Database

70
Assessment of the repair and damage of DNA induced by parent and reduced RSU-1069, a 2-nitroimidazole-aziridine
1989-04-01

The cellular repair and damage of DNA induced by parent and reduced RSU-1069, a 2-nitroimidazole-aziridine, was assessed at both the molecular and cellular level. At the molecular level, after in vitro incubation with parent or reduced RSU-1069, plasmid DNA was transfected into Escherichia coli (AB1157) with subsequent selection for gene expression. For ...

Energy Citations Database

71
THE TUBE STRESS-STRAIN PROPERTIES OF BRITTLE MATERIALS TO 5000 F. Sixth Monthly Report
1960-11-10

Additional furnace work using the 25-kw induction power supply indicated reliable long-time performance at 4500 deg F. The detailed check out of the prototype, flat, gas bearing was completed. Bearing operatlon was very stable at 0.0005-in. gap. The design of the spherical bearing was begun. The grips were checked with a ...

Energy Citations Database

72
Experimental deformation of synthetic magnetite-bearing calcite sandstones - Effects on remanence, bulk magnetic properties, and magnetic anisotropy
1993-01-01

The simultaneous effects of deformation on the magnetic remanence, bulk magnetic properties, and magnetic anisotropy of magnetite-bearing calcite sandstones were investigated in a set of synthetic magnetite-bearing samples prepared as described by Borradaile and Alford (1987). Experimental deformations of synthetic sandstone analogs containing 40-micron ...

NASA Astrophysics Data System (ADS)

73
Characterization of canine adenovirus type 1 isolated from American black bears.
1988-06-01

Viruses recovered from tissues taken at necropsy from American black bears were examined by use of immunofluorescence with polyclonal and monoclonal antibodies, virus neutralization with monoclonal antibodies, and restriction endonuclease analyses of the viral genomes. With these techniques, viruses were determined to be canine adenovirus type 1. Seronegative dogs that were ...

PubMed

74
Isotype profiles of anti-influenza antibodies in mice bearing the xid defect
1985-02-01

The humoral response to influenza A/PR8 virus was examined in the CAB/N and C/sub 3/J.xid strains of mice, both of which bear an X-linked genetic defect (xid), and in strains lacking this defect. Hemagglutination-inhibiting antibody titers and measurement of virus-specific antibodies by solid-phase radioimmunoassay indicated that the ...

Energy Citations Database

75
DETERMINATION OF THE TRUE STRESS-STRAIN PROPERTIES OF BRITTLE MATERIALS TO 5000 F. Ninth Monthly Report
1962-05-17

The development of the system and techniques to permit determination of strains at right angle to the axis of loading in the specimen was continued. An additional optical system was developed to accept the lateral image of the gage points and project a vertical image of the same proportion to the apertures of the strain analyzer. Using a dummy ...

Energy Citations Database

76
Technical specifications for the successful fabrication of laminated seismic isolation bearings
1992-01-01

High damping laminated elastomeric bearings are becoming one of the preferred devices for isolating large buildings and structures. IN the United States, the current reference design for the Advanced Liquid Metal Reactor uses laminated bearings for seismic isolation. These bearing are constructed from alternating layers of rubber and ...

DOE Information Bridge

77
Technical specifications for the successful fabrication of laminated seismic isolation bearings
1992-05-01

High damping laminated elastomeric bearings are becoming one of the preferred devices for isolating large buildings and structures. IN the United States, the current reference design for the Advanced Liquid Metal Reactor uses laminated bearings for seismic isolation. These bearing are constructed from alternating layers of rubber and ...

DOE Information Bridge

78
Pin-Contact Behaviour of Composite Sandwich Structures Under Compressive Bearing Load
2011-06-01

The aim this research is to investigate the pin-sandwich contact behaviour of some sandwich composites structures when submitted to compressive bearing loads. A preliminary set of flatwise and edgewise compressive tests and three point flexural tests were performed to get information on the mechanical behaviour of the sandwich structures under different load conditions. The ...

NASA Astrophysics Data System (ADS)

79
Effects of the Elastic Strain and Dilation in the Big End Journals
2007-01-01

The heating and mechanics effects due to the temperatures and pressures in bearings were largely studied these last years but separately; few studies are devoted to the two phenomena acting together in dynamic mode, such as the crankshaft and rod bearings; where the requirements are very severe. In order to approach reality and confronting with other ...

NASA Astrophysics Data System (ADS)

80
Safety of Brucella abortus strain RB51 in black bears.
2004-07-01

In two studies conducted from October 1999 to March 2000 and December 2000 to April 2001, adult black bears (Ursus americanus) were orally inoculated with 1.4-3.1 x 10(10) colony-forming units (CFU) of Brucella abortus strain RB51 (SRB51, n=12) or 2 ml of 0.15 M NaCl solution (saline, n=11). We did not detect a difference (P>0.05) in antibody titers to ...

PubMed

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81
Micromechanics of deformation of metallic-glass�matrix composites from in situ synchrotron strain measurements and

with non-linear isotropic hardening was chosen to model the constitu- tive behavior of the Ta yielding, the particles do not strain harden significantly; we show that this is due to an increasingly-bearing applications. The lack of strain hardening, however, means that single-phase metallic glasses experience shear

E-print Network

82
Inducing and imaging thermal strain using a single ultrasound linear array.
2007-09-01

For the first time, the feasibility of inducing and imaging thermal strain using an ultrasound imaging array is demonstrated. A commercial ultrasound scanner was used to heat and image a gelatin phantom with a cylindrical rubber inclusion. The inclusion was successfully characterized as an oil-bearing material using thermal strain ...

PubMed

83
Genome sequence of strain IMCC9480, a xanthorhodopsin-bearing betaproteobacterium isolated from the Arctic Ocean.
2011-05-13

Strain IMCC9480 is a novel member of the family Oxalobacteraceae of the Betaproteobacteria, isolated from the Arctic Ocean by dilution-to-extinction culturing. Here we present the draft genome sequence of strain IMCC9480. The genome is predicted to contain genes for xanthorhodopsin, retinoid biosynthesis, carbon monoxide dehydrogenase, and C(1) metabolism. ...

PubMed

84
Genome Sequence of Strain IMCC9480, a Xanthorhodopsin-Bearing Betaproteobacterium Isolated from the Arctic Ocean?
2011-07-01

Strain IMCC9480 is a novel member of the family Oxalobacteraceae of the Betaproteobacteria, isolated from the Arctic Ocean by dilution-to-extinction culturing. Here we present the draft genome sequence of strain IMCC9480. The genome is predicted to contain genes for xanthorhodopsin, retinoid biosynthesis, carbon monoxide dehydrogenase, and ...

PubMed Central

85
A 65-Kilobase Pathogenicity Island Is Unique to Philadelphia-1 Strains of Legionella pneumophila
2003-08-01

Nucleotide sequence analysis of an ?80-kb genomic region revealed an ?65-kb locus that bears hallmarks of a pathogenicity island. This locus includes homologues of a type IV secretion system, mobile genetic elements, and known virulence factors. Comparative studies with other Legionella pneumophila strains and serogroups indicated that this ?65-kb locus is ...

PubMed Central

86
Summary of experimental tests of elastomeric seismic isolation bearings for use in nuclear reactor plants
1992-01-01

This paper describes an experimental test program for isolator bearings which was developed to help establish the viability of using laminated elastomer bearings for base isolation of nuclear reactor plants. The goal of the test program is to determine the performance characteristics of laminated seismic isolation bearings under a wide ...

DOE Information Bridge

87
Summary of experimental tests of elastomeric seismic isolation bearings for use in nuclear reactor plants
1992-05-01

This paper describes an experimental test program for isolator bearings which was developed to help establish the viability of using laminated elastomer bearings for base isolation of nuclear reactor plants. The goal of the test program is to determine the performance characteristics of laminated seismic isolation bearings under a wide ...

DOE Information Bridge

88
Attenuation of virulence by P and V plasmids in Vibrio cholerae: strains suitable for oral immunization*
1979-01-01

In a virulent strain of Vibrio cholerae, KB9, P and V plasmids were introduced by bacterial conjugation. Characterization of PV isolates and systematic screening of them in animal models of cholera revealed that a large number of PV isolates were non-pathogenic, owing to the loss of ability to synthesize toxin. Results obtained with two such strains, ...

PubMed Central

89
Prevalence, characterization and clonal analysis of Escherichia coli O157: non-H7 serotypes that carry eae alleles.
2010-04-14

We examined O157:non-H7 strains isolated from various sources and geographical locations and found 15/57 strains to carry eae alleles, including alpha, beta, epsilon and kappa/delta, suggesting that these strains may be prevalent. All strains were serologically and genetically confirmed to be O157, but none were ...

PubMed

90
Elasto-plastic analysis of circular tunnel based on strain nonlinear softening
2008-11-01

The elastic-nonlinear softening-residual plastic surrounding rock mode1 is analyzed. According to the total strain theory, the relationship between equivalent stress and equivalent strain is deduced from uniaxial compression of practical rock. The relational expressions are related with triaxial stress (??, ?z, and ?r) and triaxial ...

NASA Astrophysics Data System (ADS)

91
Theory of Anion-Substituted Nitrogen-Bearing III-V Alloys
1998-07-20

... Abstract : Because of the large bond-length mismatch between N and P or As, enormous internal strains strongly inhibit the miscibility of the latter ...

DTIC Science & Technology

92
THE TRUE STRESS-STRAIN PROPERTIES OF BRITTLE MATERIALS TO 5000 F . Second Monthly Status Letter
1960-07-01

Characteristics of induction heating of graphite balls with thermal shielding of graphite, molybdenum, carbon steel, and austenitic stainless steel are discussed. A literature study on air bearings was continued. (M.C.G.)

Energy Citations Database

93
Some Thoughts on Material Stability.
1981-01-01

The paper is concerned with some underlying physical considerations which bear on the development of restrictions on the strain-energy function for an elastic material which may undergo finite deformations. Some comments are also made on the possible use ...

National Technical Information Service (NTIS)

94
Relationship of Pre-1918 Avian Influenza HA and NP Sequences to Subsequent Avian Influenza Strains

... the Bear River, UT�a juvenile Cinnamon teal (Anas cyanoptera) on August 11, 1916; a Ruddy duck (Oxyura jamaicensis) on August 11, 1916; a Greenwing teal (Anas crecca) on September 24, 1916; and a Bufflehea...

NBII National Biological Information Infrastructure

95
NA~A-~p,-17070r - NASA Technical Report Server (NTRS)

frOta 11% for the gDbal bearing to 40% for the HPV of lDcoael 718. ..... function of such a joint is to prevent the excessive strain of an associated bel - ...

NASA Website

96
Microbial Interactions Chapter: Binding and Entry of DNA in Bacterial Transformation.
1977-01-01

Genetic transformation of bacteria by DNA released from cells of a related strain is discussed. The mechanism by which the giant information-bearing molecules of DNA are transported into the bacterial cell was investigated. It was concluded that the overa...

National Technical Information Service (NTIS)

97
Hydraulic Engineering. Volume 91, 1969.
1971-01-01

Contents: Erosive and sediment bearing flows; Dam hydrodynamics; Fluid mechanics; Power and pumping plants; Cements, concretes, and aggregates; Stress and strength of hydraulic structures; Strain and deformation; Seepage and cracks; Rockfill structures; T...

National Technical Information Service (NTIS)

98
Geographical Distribution of Genotypic and Phenotypic Markers Among Bacillus Anthracis Isolates and Related Species by Historical Movement and Horizontal Transfer.
2009-01-01

The geographical distribution of Bacillus anthracis strains and isolates bearing some of the same genetic markers as the Amerithrax Ames isolate was examined and evaluated. At least one mechanism for the horizontal movement of genetic markers was shown am...

National Technical Information Service (NTIS)

99
Comparison of Plasmid Transformation and the Cloning of Recombinant DNA in Streptococcus Pneumoniae and Bacillus Subtilis.
1981-01-01

The molecular cloning of the malM gene of Streptococcus pneumoniae in a strain of S. pneumoniae bearing a deletion of the chromosomal mal region enabled us to investigate the feasibility of recombinant DNA cloning also in the presence of chromosomal homol...

National Technical Information Service (NTIS)

100
Building foundations under difficult geological conditions. Seminars of Finnish and Soviet experts
1980-01-01

The stress-strain properties of soils are investigated. The bearing capacity of plastically nonhomogeneous deep foundations limited and unlimited by rigid underlayers of soft underlying layers are studied. The subsoil improvement by means of vertical drainage is studied.

NASA Astrophysics Data System (ADS)

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101
Antimicrobial resistance markers of class 1 and class 2 integron-bearing Escherichia coli from irrigation water and sediments.
2003-07-01

Municipal and agricultural pollution affects the Rio Grande, a river that separates the United States from Mexico. Three hundred and twenty-two Escherichia coli isolates were examined for multiple antibiotic resistance phenotypes and the prevalence of class 1 and class 2 integron sequences. Thirty-two (10%) of the isolates were resistant to multiple antibiotics. Four (13%) of these isolates ...

PubMed

102
Anthracite mechanical mining investigations. Progress report 9: measurement of loads borne by underground-roadway supports
1953-01-01

Tests made by Bureau of an electric strain gage for measuring the roof load that a steel shield, designed by Bureau, will bear when used as an underground-roadway support in pitching anthracite beds are described.

Energy Citations Database

103
FRICTION BEARING,
1965-07-23

... ANTIFRICTION BEARINGS, JOURNAL BEARINGS), (*JOURNAL BEARINGS, ANTIFRICTION BEARINGS), BUSHINGS, PATENTS, USSR. ...

DTIC Science & Technology

104
Microstructure of halato telechelic polymers bearing group IVb metal carboxylate end-groups

-strain tensile moduli of carboxy-telechelic polyisoprenes and the modulus enhancement has been attributed1721 Microstructure of halato telechelic polymers bearing group IVb metal carboxylate end structure within the ionic aggregates of a carboxylato-telechelic ionomer neutralized with an excess

E-print Network

105
Evaluation of Fiberglass and Steel Reinforced Elastomeric Bridge Bearing Pads.
1982-01-01

The results of tests conducted on 1-1/2 inch thick reinforced neoprene bridge bearing pads having shape factors of 3, 6, 9 and 12 are presented herein. The tests included compressive stress versus strain, creep, translation and ultimate compressive streng...

National Technical Information Service (NTIS)

106
The influence of temperature and strain rate on the tensile and compressive constitutive response of four fluoropolymers
2006-08-01

Fluoropolymers posses several advantageous properties for load-bearing structural components including higher strength at elevated temperatures and higher toughness at lowered temperatures. Stress-strain studies have been conducted on two homopolymers (Teflon (PTFE) and KEL-F 81 (PCTFE)) and two copolymers (KEL-F 800 and THV 500G) as a function of ...

NASA Astrophysics Data System (ADS)

107
Serotypes of Plasmodium falciparum defined by immune serum inhibition of in vitro growth*
1985-01-01

In vitro growth inhibition assays were used to detect antigenic differences among geographically distinct strains of Plasmodium falciparum. Owl monkeys were immunized against the Camp and FCR-3/FMG strains of P. falciparum by infection, drug treatment, and rechallenge with homologous parasites. Camp-immune monkey serum was used to inhibit the in vitro ...

PubMed Central

108
An endosymbiont positively modulates ornithine decarboxylase in host trypanosomatids
2006-05-05

Summary: Some trypanosomatids, such as Crithidia deanei, are endosymbiont-containing species. Aposymbiotic strains are obtained after antibiotic treatment, revealing interesting aspects of this symbiotic association. Ornithine decarboxylase (ODC) promotes polyamine biosynthesis and contributes to cell proliferation. Here, we show that ODC activity is higher in ...

Energy Citations Database

109
Potential causes for the non-Newtonian rheology of crystal-bearing magmas
2011-05-01

Experimental studies indicate that crystal-bearing magma exhibits non-Newtonian behavior at high strain rates and solid fractions. We use a zero-dimensional (0-D) inversion model to reevaluate rheological parameters and shear heating effects from laboratory data on crystal-bearing magma. The results indicate non-Newtonian behavior with ...

NASA Astrophysics Data System (ADS)

110
Photoreactivation of ultraviolet-irradiated, plasmid-bearing and plasmid-free strains of bacillus anthracis
1985-12-19

The effects of toxin- and capsule-encoding plasmids on the kinetics of UV inactivation of various strains of Bacillus anthracis were investigated. Plasmids pX01 and pX02 had no effect on bacterial UV sensitivity or photoreactivation. Vegetative cells were capable of photoreactivation, but photo-induced repair of UV damage absent in B. anthracis Sterne spores.

Energy Citations Database

111
Engineers Edge: Engineering Data and Knowledge Resources
2006-12-01

A commercial site that provides tutorials, formulas, design guide, and online calculators for a range of design and engineering topics include Reynold's number, pipe friction, mechanical tolerances, beam bending, springs, thread stress area, strain gauge application, plastic snap fit application, pressure vessel, battery design, bearings, electric motor, ...

NSDL National Science Digital Library

112
Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine.
2010-06-04

A novel actinomycete, strain X4(T), was isolated from a phosphate mine in Youssoufia, 100 km north of Marrakesh, Morocco. The taxonomic status of this strain was evaluated by a polyphasic approach. Strain X4(T) had white aerial mycelium with Rectiflexibiles spore chains bearing smooth-surfaced spores and did not ...

PubMed

113
The influence of design, materials and kinematics on the in vitro wear of total knee replacements.
2005-02-01

Debris-induced osteolysis due to surface wear of ultra high molecular weight polyethylene (UHMWPE) bearings is a potential long-term failure mechanism of total knee replacements (TKR). This study investigated the effect of prosthesis design, kinematics and bearing material on the wear of UHMWPE bearings using a physiological knee ...

PubMed

114
Use of a single procedure for selective enrichment, isolation, and identification of plasmid-bearing virulent Yersinia enterocolitica of various serotypes from pork samples.
1997-05-01

Many selective enrichment methods for the isolation of Yersinia enterocolitica from foods have been described. However, no single isolation procedure has been described for the recovery and identification of various plasmid-bearing serotypes. A single improved procedure for selective enrichment, isolation, identification, and maintenance of plasmid-bearing ...

PubMed Central

115
The development of a bearing spectral analyzer and algorithms to detect turbopump bearing wear from deflectometer and strain gage data
1992-07-01

Over the last several years, Rocketdyne has actively developed condition and health monitoring techniques and their elements for rocket engine components, specifically high pressure turbopumps. Of key interest is the development of bearing signature analysis systems for real-time monitoring of the cryogen-cooled turbopump shaft bearings, which spin at ...

Energy Citations Database

116
Production of methionine ?- lyase in recombinant Citrobacter freundii bearing the hemoglobin gene.
2011-09-01

The production of antileukemic enzyme methionine ?-lyase (MGL) in distinctly related bacteria, Citrobacter freundii and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. This study concerns the potential of Citrobacter freundii expressing the Vitreoscilla hemoglobin gene (vgb) for the methionine ?- liyase production. Methionine ?- liyase production by Citrobacter ...

PubMed

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