To facilitate the diagnosis of enterotoxigenic Escherichia coli (ETEC) infections in humans, we developed and evaluated real-time fluorescence PCR assays for the Roche LightCycler (LC) against the enterotoxin genes commonly present in strains associated with human illness. Separate LC-PCR assays with identical cycling conditions were designed for the type I heat-labile ...
PubMed Central
Seventy-four E. coli strains isolated from piglets with diarrhea or edema disease in Spain were serotyped and examined for production of heat-labile (LT) and heat-stable (ST) enterotoxins (LT-I, LT-II, STaH, STaP, and STb) and verotoxins (VT1, VT2, and VT2v = VTe) by phenotypic (Vero cell assay and infant mouse test) and genotypic (colony hybridization and ...
Cholera, travelers' diarrhea, or colibacillosis in pigs can possibly be prevented or attenuated by dietary provision of competitive inhibitors that react with the GM1-binding sites of the enterotoxins cholera toxin (CT), human Escherichia coli heat-labile enterotoxin of serogroup I (LTh-I), and porcine LT-I (LTp-I). The interfering ...
PubMed
Cholera toxin (CT) produced by Vibrio cholerae and heat-labile enterotoxin (LT-I), produced by enterotoxigenic Escherichia coli, are AB5 heterohexamers with an ADP-ribosylating A subunit and a GM1 receptor binding B pentamer. These toxins are among the most potent mucosal adjuvants known and, hence, are of interest both for the development of ...
Escherichia coli heat-labile enterotoxins (LT) are responsible in part for "traveler's diarrhea" and related diarrheal illnesses. The family of LTs comprises two serogroups termed LT-I and LT-II; each serogroup includes two or more antigenic variants. The effects of LTs result from ADP ribosylation of Gs alpha, a stimulatory component of adenylyl cyclase; ...
Heat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. ...
... Abstract : In the course of investigating E. coli enterotoxins, it was discovered that trypsin treatment of partially purified enterotoxin from strain H197 ...
DTIC Science & Technology
Two families of bacterial heat-labile enterotoxins (HLTs) have been described: the type I HLTs are comprised of cholera toxin (CT) of Vibrio cholerae, LT-I of Escherichia coli, and several related HLTs; the type II HLTs are comprised of LT-IIa and LT-IIb. Herein, we report LT-IIc, a new type II HLT encoded from an enterotoxigenic E. coli (ETEC) strain ...
... was solved. The trisaccharide is the head group of glysophingolipid, GM3. Descriptors : *ENTEROTOXINS, *STAPHYLOCOCCUS ...
... Title : THE GENERATION OF STAPHYLOCOCCAL ENTEROTOXIN IN SAUSAGE MEAT AND PREPARED MEAT PRODUCTS,. ...
... The mode of action of these enterotoxins is unknown, but it does not appear that the adenyl cyclase-adenosine monophosphate system is involved. ...
... Accession Number : AD0837588. Title : REPORT ON STAPHYLOCOCCUS ENTEROTOXINS. 4. RESEARCH ON STAPHYLOCOCCUS ...
... Title : INHIBITION OF STAPHYLOCOCCAL ENTEROTOXIN B FORMATION BY CELL WALL BLOCKING AGENTS AND OTHER COMPOUNDS. ...
... Title : INFLUENCE OF STAPHYLOCOCCAL ENTEROTOXIN ON INFLUENZA VIRUS INFECTIVITY IN THE EMBRYONATED EGG. ...
... create enterotoxin homologous to the antitoxin of strain 32/54 ... STAPHYLOCOCCUS, TOXINS AND ANTITOXINS), IDENTIFICATION, ...
... for a cell system with ... Descriptors : *MOLECULAR STRUCTURE, * ENTEROTOXINS ... GENES, CLONES, CELLS(BIOLOGY), SEROLOGY, ...
... ENTEROTOXIN B ON COMPLEX OPERANT BEHAVIOR IN ... MOTOR REACTIONS, CONTROLLED ATMOSPHERES, CONDITIONED RESPONSE. ...
... 2. Concerning the Effect of Staphylococcal Enterotoxins on Nematodes (Beitrag zum Nachweis von Staphylokokkenenterotoxin. ...
The circular dichroism (CD) of staphylococcal enterotoxins A, B and C was measured. The CD of enterotoxins B and C were almost identical from 250-320 nm, but differed from the CD of enterotoxin A. The spectrum of enterotoxin A in this wavelength region co...
National Technical Information Service (NTIS)
The type I and type II heat-labile enterotoxins (LT-I and LT-II) are strong mucosal adjuvants when they are coadministered with soluble antigens. Nonetheless, data on the parenteral adjuvant activities of LT-II are still limited. Particularly, no previous study has evaluated the adjuvant effects and induced inflammatory reactions of LT-II holotoxins or ...
... A U 2 6 IS66 IM~ii~i mflnr..... Effect of Staphylococcal Enterotoxin on Dermal Reactivity to A ... Enterotoxin on Dermal Reactivity of Rabbits to ...
Liu, XL Wang J Volf Alloy Sheathed Bi-2223 HTS Tapes for Current Leads HK Liu, M Apperley #12;9 Energy for Electric Vehicles HK Liu, D Bradhurst Masters A Howes Stoichiometric and Non-Stoichiometric AB5 Type Alloys Supervisors R Baker Zeeman and Piezospectroscopy of Antimony and Aluminum in Germanium P Fisher, R Vickers D
E-print Network
Faeces samples from diarrhoeic and non-diarrhoeic lambs and goat kids aged 1-45 days were examined for enteric pathogens. Cryptosporidium parvum was detected in both diarrhoeic lambs (45%) and goat kids (42%) but not in non-diarrhoeic animals. F5+ (K99+) and/or F41+ Escherichia coli strains were isolated from 26% and 22% of the diarrhoeic lambs and goat kids, respectively, although these strains, ...
... DERMAL REACTIVITY TO EPINEPHRINE I ... I. Effect of Intravenous Injection of Enterotoxin on the Dcrmal Reactivity of Rabbits to Epinephrine ..... ...
... by specific anti-enterotoxin antibodies in the supernatants of in vitro cultured intestinal fragments and the synthesized immunoglobulin was IgA. ...
... of a lesion attributable to enterotoxin in man is that of Palmer I who studied "peroral biopsy specimens of gastric (body) mucosa in patients with ...
... Accession Number : ADA047796. Title : Protease Inhibitors Suppress Enterotoxin B Formation by 'Staphylococcus aureus.'. ...
... Accession Number : ADA070543. Title : Immunogenicity of Formaldehyde- Inactivated Enterotoxins A and C1 of 'Staphylococcus aureus',. ...
... Accession Number : ADA083408. Title : Enterotoxin B Formation by Fermentation Mutants of Staphylococcus aureus,. Corporate ...
... TECHNICAL MANUSCRIPT 508 EFFECT OF FORMALDEHYDE ON STAPHYLOCOCCAL I ENTEROTOXIN B: IMMUNOGENICj ICIIYI ACC UAT ...
... Accession Number : AD0850330. Title : Effect of Formaldehyde on Staphylococcal Enterotoxin B: Immunogenic Activity in Macaca Mulatta. ...
... toxicological effects of staphylococcal enterotoxin B, a compound frequently associated with food poisoning, on operant conditioning schedules of ...
... Title : Detection of Staphylococcus Aureus Enterotoxin A and B Genes with PCR-EIA and a Hand-Held Electrochemical Sensor. ...
One hundred of the most frequently referenced and historically interesting reports related to the detection of staphylococcal enterotoxins are annotated. (Author)
... the methods described for the production of enterotoxin ... F, typical formation of reference line of precipitation in slide test control system. ...
Center for Food Safety and Applied Nutrition (CFSAN)
The observation of neutrinos from SN1987A [1,2], in fair agreement with predictions ... Finally, these neutrinos are trapped in the core and undergo ...
NASA Website
ruthenium, pallarfiom and antimony in the stoichiomettic ratio and sabsequcrrt ..... of p-type lli2Tes-based alloys increases and varies as l/T. I;crr ...
Different procedures commonly used for extraction, purification, and concentration of staphylococcal enterotoxins from foods were investigated with 131I- and 125I-labeled staphylococcal enterotoxin A. Loss of labeled enterotoxin A was compared with loss of total nitrogen. The results showed that in most of the common procedures, such ...
Fermentation of glucose-xylose mixtures to ethanol was investigated in batch and continuous experiments using immobilized recombinant <i>Zymomonas mobilis</i> CP4(pZB5). This microorganism was immobilized by ent...
Mar 29, 2007 ... Archive if we fall w/in the local time limits IF ( LT(I) >= HR1_OTH .and. LT(I) <= HR2_OTH ) THEN LTOTH(I,J) = 1 CTOTH(I,J) = CTOTH(I,J) + 1 ...
A sensitive double-antibody radioimmunoassay for staphylococcal enterotoxin C$sub 2$ is described. The assay procedure employs anti-rabbit gamma globulin, prepared in goats, to precipitate the antigen-antibody complex of enterotoxin C$sub 2$ and anti-enterotoxin C$sub 2$. The test is sensitive to 100 pg of ...
Energy Citations Database
An automated and rapid method for detection of staphylococcal enterotoxins (SE) is needed. A sandwich assay was developed using a surface plasmon resonance (SPR) biosensor for detection of staphylococcal enterotoxin A (SEA) at subpicomolar concentration. Assay conditions were optimized for capturing...
Technology Transfer Automated Retrieval System (TEKTRAN)
The extent and rate of reduction of aqueous staphylococcal enterotoxin B by dithiothreitol in the absence of the denaturant guanidine hydrochloride were directly proportional to the extent and rate of enterotoxin B unfolding. Thus, the single disulfide bo...
The three-dimensional structure of staphylococcal enterotoxin C2 has been determined at 2.7 A resolution by X-ray diffraction, while the structures of enterotoxins A and E have been modelled based on their sequence homology to other staphylococcal enterot...
Certain protease inhibitors depress enterotoxin B production without diminishing growth of the organism. Enterotoxin synthesis by concentrated, resting cells is also decreased by the same inhibitors. The results suggest that a protealytic cleavage step is...
It has been determined that representative culture filtrates from two different strains (H197 and 74-114) of enterotoxigenic E. coli contain at least two different forms of heat-labile enterotoxin. One form of enterotoxin appears to be complex and partial...
The report summarizes research performed in the areas of toxin production, assay and purification. One of the major aspects of the work has been the demonstration that Escherichia coli enterotoxin(s), like cholera toxin, cause vascular permeability change...
Rhesus monkeys given 150 micrograms of purified staphylococcal enterotoxin B developed acute gastroenteritis. This was well developed at 2 hours, maximal at 4 to 8 hours and rapidly regressed 12 to 72 hours after enterotoxin administration. These features...
The ammonium sulfate coprecipitation technique of Farr was applied in a study of the purified enterotoxins of Staphylococcus aureus. Ammonium sulfate coprecipitation of iodine-131-labeled enterotoxins A, B, and C, with the use of a 1.6 M concentration of ...
The ammonium sulfate coprecipitation technique of Farr was applied to purified enterotoxins of Staphylococcus aureus. Studies with iodine 131-labeled enterotoxins A, B, and C, using 1.6 M ammonium sulfate for coprecipitation, revealed differences in the a...
The effect of Shigella dysenteriae I enterotoxin on adenylate cyclase activity in rabbit ileal mucosa was studied under various in vitro and in vivo conditions. Activation of adenylate cyclase by Shigella enterotoxin was observed only when substrate (ATP)...
The half disappearance time (t 1/2) of highly purified, I131 labeled, staphylococcal enterotoxin B (SEB-I131) from the blood of male albino rats was studied as a means for the assay of specific staphylococcal enterotoxin B antisera (SEBA). A prolongation ...
The experiments based on morphologic examination and clearance kinetics clearly indicate that the kidney is the predominant site of toxin localization after intravenous injection of Staphylococcus enterotoxin B. Experiments show that the majority of the t...
... SO4 . 2. Adsorption chromatography with Amberlite XAD-2, the toxin is eluted with 1% acetic acid, 99% methanol. 3. Gel ...
this page to a friend Share An enterotoxin is a harmful substance produced by certain bacteria that is specifically dangerous to parts of your gastrointestinal tract. The substance...
Science.gov Websites
Three of 36 raw milk isolates of Yersinia enterocolitica produced enterotoxin in milk at 25 degrees C, but not at 4 degrees C. No strain tested could survive pasteurization.
... Title : Effects of Exogenous Fatty Acids on Growth and Enterotoxin B Formation by 'Staphylococcus aureus' 14458 and Its Membrane Mutant. ...
... described for the recovery of enterotoxin from bacterial supernatants of Staphylococcus strain S-6 by use of the ion exchange resin Amberlite XE-64 ...
... Accession Number : AD0767789. Title : 'Staphylococcus aureus' Enterotoxin B Release (Excretion) under Controlled Conditions of Fermentation. ...
Staphylococcus aureus is the most prevalent bovine mastitis pathogen in Argentina. The ability of this organism to produce enterotoxins is linked to staphylococcal food poisoning. Staphylococcal enterotoxins are low molecular weight proteins, highly resistant to heat and proteolytic enzyme activity. The aim of this study was to determine the ability to ...
... Gastritis was most severe in the antral and fundic mucosa and was less severe in the area of the stomach containing parietal cells. ...
