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1
Gradual Ordering in Red Abalone Nacre
2008-09-03

Red abalone (Haliotis rufescens) nacre is a layered composite biomineral that contains crystalline aragonite tablets confined by organic layers. Nacre is intensely studied because its biologically controlled microarchitecture gives rise to remarkable strength and toughness, but the mechanisms leading to its formation are not well ...

Energy Citations Database

2
Mechanical properties of nacre constituents and their impact on mechanical performance

Mechanical properties of nacre constituents and their impact on mechanical performance Fran�ois) The mechanical properties of nacre constituents from red abalone were investigated. Electron microscopy studies that other interface features must be responsible for the enhanced performance of nacre over its ...

E-print Network

3
ARTICLES Model for the robust mechanical behavior of nacre

ARTICLES Model for the robust mechanical behavior of nacre A.G. Evans and Z. Suo Department) The inelastic deformation of nacre that leads to its structural robustness has been characterized in a recent the axis of the aragonite plates. The model is based on observations for abalone nacre

E-print Network

4
Synthesis of Nacre-Like Structures using Novel Fabrication ...
2010-02-18

... This proposal addresses the nacre in the abalone shell and the methods that will be taken to fabricate and test a bioinspired material based on its ...

DTIC Science & Technology

5
Synthesis of Nacre-Like Structures using Novel Fabrication Techniques.
2010-01-01

This proposal addresses the nacre in the abalone shell and the methods that will be taken to fabricate and test a bioinspired material based on its structure. Our objectives are 2-fold: (1) to fabricate a nano/micro- laminate that mimics the nacre in the ...

National Technical Information Service (NTIS)

6
Abalone nacre insoluble matrix induces growth of flat and oriented aragonite crystals.
2006-03-31

Mollusc shell formation takes place in a preformed extracellular matrix, composed of insoluble chitin, coated with proteins and dissolved macromolecules. The water-soluble matrix is known to have a strong influence on the growth of CaCO(3), whereas the role of the insoluble matrix on mineralization is unclear. Therefore, we mineralized the EDTA (ethylenediaminetetraacetic ...

PubMed

7
Structural and functional biological materials: Abalone nacre, sharp materials, and abalone foot adhesion
2008-01-01

A three-part study of lessons from nature is presented through the examination of various biological materials, with an emphasis on materials from the mollusk Haliotis rufescens, commonly referred to as the red abalone. The three categories presented are: structural hierarchy, self-assembly, and functionality. Ocean mollusk shells are composed of aragonite/calcite crystals ...

NASA Astrophysics Data System (ADS)

8
ICGA ABALONE TOURNAMENT Tino Werner1

that the only two participants in the Abalone tournament were Peer Sommerlung with his program Nacre and Tino that game could have been defined as a tie but in the end, when time was almost up for both players, Nacre #12;Game Black-White Score Time Used Moves 1 Nacre-AbaPro 2-6 15:00 - 22:50 55 2 ...

E-print Network

9
Acceleration of calcite kinetics by abalone nacre proteins
2005-06-09

The fascinating shapes and hierarchical designs of biomineralized structures have long been an inspiration to materials scientists because of the potential they suggest for biomolecular control over synthesis of crystalline materials. One prevailing view is that mineral-associated macromolecules are responsible for initiating and stabilizing non-equilibrium crystal polymorphs and morphologies ...

DOE Information Bridge

10
Crystallography, microstructure, and implications for the formation of the biomineralized system, nacre in red abalone
1998-01-01

Biomineralized hard tissues (organic/inorganic composites) show extraordinary specialization and control over their form and microstructure. Biocomposites have crystallites of organized shape, distribution, orientation, and size within an organic framework. The resulting optimized microstructures provide an excellent combination of physical properties. The research goal here was to investigate ...

NASA Astrophysics Data System (ADS)

11
0101748 - Ancient techniques for new materials - NASA

His research is based on the model of how an abalone builds the nacre, also called mother-of-pearl, inside its shell. Strong thin coatings, or lamellae, ...

NASA Website

12
The amino-acid sequence of the abalone (Haliotis laevigata) nacre protein perlucin. Detection of a functional C-type lectin domain with galactose/mannose specificity.
2000-08-01

Perlucin isolated from abalone nacre consists of 155 amino acids including a glycosylated asparagine. The sequence of the first 130 amino acids shows a high similarity to the C-type carbohydrate-recognition domains of asialoglycoprotein receptors and other members of the group of C-type lectins but also a weaker similarity to related ...

PubMed

13
Perlwapin, an abalone nacre protein with three four-disulfide core (whey acidic protein) domains, inhibits the growth of calcium carbonate crystals.
2006-07-21

We have isolated a new protein from the nacreous layer of the shell of the sea snail Haliotis laevigata (abalone). Amino acid sequence analysis showed the protein to consist of 134 amino acids and to contain three sequence repeats of approximately 40 amino acids which were very similar to the well-known whey acidic ...

PubMed

14
Perlwapin, an Abalone Nacre Protein with Three Four-Disulfide Core (Whey Acidic Protein) Domains, Inhibits the Growth of Calcium Carbonate Crystals
2006-10-01

We have isolated a new protein from the nacreous layer of the shell of the sea snail Haliotis laevigata (abalone). Amino acid sequence analysis showed the protein to consist of 134 amino acids and to contain three sequence repeats of ?40 amino acids which were very similar to the well-known whey acidic protein ...

PubMed Central

15
Molecular approaches to understand biomineralization of shell nacreous layer.
2011-01-01

The nacreous layer of molluskan shells, which consists of highly oriented aragonitic crystals and an organic matrix (including chitin and proteins), is a product of biomineralization. This paper briefly introduces the recent research advances on nacre biomineralization of shells from bivalves and gastropods, which mainly focus on analysis of the micro- and ...

PubMed

16
Polarization-dependent imaging contrast in abalone shells Rebecca A. Metzler,1 Dong Zhou,1 Mike Abrecht,2 Jau-Wern Chiou,3 Jinghua Guo,4 Daniel Ariosa,5 Susan N. Coppersmith,1

simulated spectra. PIC reveals different and unexpected aspects of nacre architecture that have inspired theoretical models for nacre formation. DOI: 10.1103/PhysRevB.77.064110 PACS number s : 61.05.cj, 87.85.J INTRODUCTION Nacre, or mother-of-pearl, is intensely studied by materi- als scientists, mineralogists

E-print Network

17
Purification and characterization of perlucin and perlustrin, two new proteins from the shell of the mollusc Haliotis laevigata.
2000-01-01

Two new proteins, named perlucin and perlustrin, with M(r) 17,000 and 13,000, respectively, were isolated from the shell of the mollusc Halotis laevigata (abalone) by ion-exchange chromatography and reversed-phase HPLC after demineralization of the shell in 10% acetic acid. The sequence of the first 32 amino acids of perlucin indicated that this ...

PubMed

18
J O U R N A L O F T H E M E C H A N I C A L B E H AV I O R O F B I O M E D I C A L M A T E R I A L S 2 ( 2 0 0 9 ) 6 0 7 � 6 1 2 available at www.sciencedirect.com

.elsevier.com/locate/jmbbm Research paper Interfacial shear strength in abalone nacre Albert Yu-Min Lina,, Marc Andr� Meyersa of the interface between tiles of aragonite in the nacre of red abalone Haliotis rufescens was investigated through of nacre when loaded parallel to the plane of ...

E-print Network

19
Statistical physics models for nacre fracture simulation
2005-10-01

Natural biological materials such as nacre (or mother-of-pearl), exhibit phenomenal fracture strength and toughness properties despite the brittle nature of their constituents. For example, nacre�s work of fracture is three orders of magnitude greater than that of a single crystal of its constituent mineral. This study investigates the fracture ...

NASA Astrophysics Data System (ADS)

20
Characterization of two molluscan crystal-modulating biomineralization proteins and identification of putative mineral binding domains.
2003-12-01

Ethylenediamine-tetraacetic acid extracted water-soluble matrix proteins in molluscan shells secreted from the mantle epithelia are believed to control crystal nucleation, morphology, orientation, and phase of the deposited mineral. Previously, atomic force microscopy demonstrated that abalone nacre proteins bind ...

PubMed

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21
Characterization of MRNP34, a novel methionine-rich nacre protein from the pearl oysters.
2011-05-18

Nacre of the Pinctada pearl oyster shells is composed of 98% CaCO(3) and 2% organic matrix. The relationship between the organic matrix and the mechanism of nacre formation currently constitutes the main focus regarding the biomineralization process. In this study, we isolated a new nacre matrix protein in P. ...

PubMed

22
Spatial and temporal resolution of interfaces, phase transitions and isolation of three families of proteins in calcium carbonate based biocomposite materials
1997-01-01

The abalone shell is a microlaminate organic-inorganic composite of mineral and biopolymers exhibiting exceptional nanoscale regularity and a strength �3,000 times greater than that of the inorganic crystals themselves. Although the integral proteins typically comprise less than 2% (by mass) of the shell, they determine the structural organization and ...

NASA Astrophysics Data System (ADS)

23
Perlinhibin, a Cysteine-, Histidine-, and Arginine-Rich Miniprotein from Abalone (Haliotis laevigata) Nacre, Inhibits In Vitro Calcium Carbonate Crystallization
2007-08-15

We have isolated a 4.785 Da protein from the nacreous layer of the sea snail Haliotis laevigata (greenlip abalone) shell after demineralization with acetic acid. The sequence of 41 amino acids was determined by Edman degradation supported by mass spectrometry. The most abundant amino acids were cysteine (19.5%), histidine (17%), and arginine (14.6%). The ...

PubMed Central

24
The interstitial crystal-nucleating sheet in molluscan Haliotis rufescens shell: a bio-polymeric composite.
2010-08-09

The interstitial green sheets in abalone shell nacre are shown to be bifacially differentiated trilaminate polymeric complexes, with glycoprotein layers sandwiching a central core containing chitin. They share some common feature with the organic matrix layers between the aragonite tablets in the nacre and the periostracum, and show ...

PubMed

25
Kidwell: Supplementary Online Material Dec 14, 2004 INTRODUCTION

by a #12;2 refractory organic matrix of fibrous protein (0.02 to 8 microns thick; S8). Microstructures (nacre S1), bivalve microstructures are either (a) high-organic (>0.5 to 5 wt.-%), comprising nacre, simple (tablets of nacre and laths of foliated and cross-lamellar microstructures, prisms of composite or ...

E-print Network

26
Dimensional analysis and parametric studies for designing artificial nacre.
2010-11-24

Nacre, the iridescent material found in Abalone shells, exhibits remarkable strength and toughness despite its composition of over 95% brittle ceramic. Its hierarchical structure over multiple length scales gives rise to its increase in toughness despite its material composition. In this work we develop a computational model of composites incorporating key ...

PubMed

27
Chemical-mechanical stability of the hierarchical structure of shell nacre
2010-02-01

The hierarchical structure and mechanical property of shell nacre are experimentally investigated from the new aspects of chemical stability and chemistry-mechanics coupling. Through chemical deproteinization or demineralization methods together with characterization techniques at micro/nano scales, it is found that the nacre of ...

NASA Astrophysics Data System (ADS)

28
Polarization-dependent imaging contrast in abalone shells
2008-02-01

Many biominerals contain micro- or nanocrystalline mineral components, organized accurately into architectures that confer the material with improved mechanical performance at the macroscopic scale. We present here an effect which enables us to observe the relative orientation of individual crystals at the submicron scale. We call it polarization-dependent imaging contrast (PIC), as it is an ...

NASA Astrophysics Data System (ADS)

29
GROWTH AND ENERGY UTILIZATION OF JUVENILE PINK ABALONE HALIOTIS CORRUGATA FED DIETS CONTAINING DIFFERENT LEVELS OF ...

... to estimate the amount of crude protein or calories consumed per g abalone. The calculated data was ... relative to diet stability. Feed intake, expressed as calories/g abalone ranged between 84�70 and wa...

NBII National Biological Information Infrastructure

30
Abalone lysin: the dissolving and evolving sperm protein

Abalone lysin: the dissolving and evolving sperm protein Nicole Kresge,1 * Victor D. Vacquier,2 and C. David Stout1 Summary Abalone sperm lysin is a non-enzymatic protein that creates a hole for sperm, and evolutionary biology to attempt to understand the molecular mechanism of fertilization in the ...

E-print Network

31
A continuous damage random thresholds model for simulating the fracture behavior of nacre.
2005-04-22

This study investigates the fracture properties of nacre using a discrete lattice model based on continuous damage random threshold fuse network. The discrete lattice topology of the model is based on nacre's unique brick and mortar microarchitecture. The mechanical behavior of each of the bonds in the discrete lattice model is governed by the ...

PubMed

32
Pmarg-Pearlin is a Matrix Protein Involved in Nacre Framework Formation in the Pearl Oyster Pinctada margaritifera.
2011-07-27

The shell of pearl oysters is organized in multiple layers of CaCO(3) crystallites packed together in an organic matrix. Relationships between the components of the organic matrix and mechanisms of nacre formation currently constitute the main focus of research into biomineralization. In this study, we characterized the pearlin protein from the oyster ...

PubMed

33
Statistical physics models for nacre fracture simulation.
2005-10-19

Natural biological materials such as nacre (or mother-of-pearl), exhibit phenomenal fracture strength and toughness properties despite the brittle nature of their constituents. For example, nacre's work of fracture is three orders of magnitude greater than that of a single crystal of its constituent mineral. This study investigates the fracture properties ...

PubMed

34
Theoretical characterization of a model of aragonite crystal orientation in red abalone nacre
2009-03-01

Nacre, commonly known as mother-of-pearl, is a remarkable biomineral that in red abalone consists of layers of 400 nm thick aragonite crystalline tablets confined by organic matrix sheets, with the [0 0 1] crystal axes of the aragonite tablets oriented to within �12� from the normal to the layer planes. Recent experiments demonstrate that greater ...

NASA Astrophysics Data System (ADS)

35
Nanoscale structure and mechanical behavior of growth lines in shell of abalone Haliotis gigantea.
2011-01-11

In the natural world, bottom-up hierarchical construction of complex structures results in materials with remarkable properties. A well known example is the nacre of mollusk shells, commonly called "mother of pearl", whose excellent strength and toughness has been the subject of research for many decades. A significant discovery has been the presence of periodic layers called ...

PubMed

36
Interfacial shear strength in abalone nacre.
2009-05-04

The shear strength of the interface between tiles of aragonite in the nacre of red abalone Haliotis rufescens was investigated through mechanical tensile and shear tests. Dog-bone shaped samples were used to determine the tensile strength of nacre when loaded parallel to the plane of growth; the mean strength was 65 MPa. Shear tests ...

PubMed

37
Continuous self-assembly of organic-inorganic nanocomposite coatings that mimic nacre
1998-07-01

Nanocomposite materials are widespread in biological systems. Perhaps the most studied is the nacre of abalone shell, an orientated coating composed of alternating layers of aragonite (CaCO3) and a biopolymer. Its laminated structure simultaneously provides strength, hardness and toughness: containing about 1vol.% polymer, nacre is ...

NASA Astrophysics Data System (ADS)

38
Nacre protein fragment templates lamellar aragonite growth.
2010-05-12

Proteins play a major role in the formation of all biominerals. In mollusk shell nacre, complex mixtures and assemblies of proteins and polysaccharides were shown to induce aragonite formation, rather than the thermodynamically favored calcite (both aragonite and calcite are CaCO(3) polymorphs). Here we used N16N, a single 30 amino ...

PubMed

39
Phylogeny of whey acidic protein (WAP) four-disulfide core proteins and their role in lower vertebrates and invertebrates.
2011-10-01

Proteins containing WAP (whey acidic protein) domains with a characteristic WFDC (WAP four-disulfide core) occur not only in mammals (including marsupials and monotremes) but also in birds, reptiles, amphibians and fish. In addition, they are present in numerous invertebrates, from cnidarians to urochordates. Many of those from non-mammalian groups are ...

PubMed

40
Role of mineral proximity on mechanics of organic macromolecules in a bio-nanocomposite
2007-01-01

Nacre, the iridescent inner layer of seashells, is a material exhibiting extraordinary mechanical properties. Nacre is a laminated nanocomposite composed of pseudohexagonal platelets that are 2 micrometer to 3 micrometer in diameter and 250 nm to 500 nm tall with an organic phase about 20 nm thick between the platelets. Although nacre ...

NASA Astrophysics Data System (ADS)

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41
The Crystal Structure of a Fusagenic Sperm Protein Reveals Extreme Surface Properties,

that is released from abalone sperm during the acrosome reaction. It coats the acrosomal process where, a 16 kDa abalone sperm protein that dissolves the vitelline envelope surrounding the egg. The two present the crystal structure of green abalone sp18 resolved to 1.86 �. Sp18 is composed of a bundle

E-print Network

42
Tablet-level origin of toughening in abalone shells and translation to synthetic composite materials.
2011-02-01

Nacre, the iridescent material in seashells, is one of many natural materials employing hierarchical structures to achieve high strength and toughness from relatively weak constituents. Incorporating these structures into composites is appealing as conventional engineering materials often sacrifice strength to improve toughness. Researchers hypothesize that ...

PubMed

43
Bone indentation recovery time correlates with bond reforming time
2001-12-01

Despite centuries of work, dating back to Galileo, the molecular basis of bone's toughness and strength remains largely a mystery. A great deal is known about bone microsctructure and the microcracks that are precursors to its fracture, but little is known about the basic mechanism for dissipating the energy of an impact to keep the bone from fracturing. Bone is a nanocomposite of hydroxyapatite ...

NASA Astrophysics Data System (ADS)

44
Mechanical Properties of Mother of Pearl in Tension
1977-04-01

Mother of pearl, or nacre, is one of a number of characteristic skeletal structures of molluscs, occurring in cephalopods, gastropods and bivalves. It consists of plates of aragonite, about 0.3 ? m thick, arranged in sheets, with a tenuous protein matrix. Mechanical tests of nacre from all three classes show that it has a tensile ...

NASA Astrophysics Data System (ADS)

45
Structure and reaction studies of biological organic and inorganic composite materials: Abalone shells, diatoms, and a unique birch bark
1998-01-01

Biopolymer/calcium carbonate composites grown on inorganic abiotic substrates implanted between the shell and the shell-secreting epithelium of live red abalones (Haliotis rufescens) results in an unusual highly (104)-oriented aggregate of microcrystalline calcite that precedes nacre deposition. Calcite of this orientation has never before been observed in ...

NASA Astrophysics Data System (ADS)

46
Molecular characterization of the 30-AA N-terminal mineral interaction domain of the biomineralization protein AP7.
2004-12-21

The AP7 protein is one of several mollusk shell proteins which are responsible for aragonite polymorph formation and stabilization within the nacre layer of the Pacific red abalone, H. rufescens. Previously, we demonstrated that the 30-AA N-terminal domain of AP7, denoted as AP7-1, exists as an unfolded sequence ...

PubMed

47
From Peptides to Proteins: Systematic Control of Net Molecular Charge and Hydrophilicity on the Kinetics of Calcite Growth
2006-12-01

The compartment-specific compositions of biologic molecules isolated from biominerals suggest that control of mineral growth may be linked to biochemical features. Here we define a systematic relationship between the ability of biomolecules in solution to promote the growth of calcite (CaCO3) and their net negative molecular charge and hydrophilicity. The degree of enhancement is dependent on ...

NASA Astrophysics Data System (ADS)

48
Towards an Understanding of the Role of Aragonite in the Mechanical Properties of Nacre
2010-08-25

Nacre, also known as mother-of-pearl, is a biocomposite material that exhibits higher strength and fracture toughness than its component materials. It derives its strength from the brick-and-mortar layering of aragonite (CaCO{sub 3}) platelets and organic binder. It is believed that the protein binder helps redistribute the stress throughout all tablets ...

DOE Information Bridge

49
Formation of framework nacre polypeptide supramolecular assemblies that nucleate polymorphs.
2011-04-19

The formation of aragonite in the mollusk shell nacre layer is linked to the assembly of framework protein complexes that interact with ?-chitin polysaccharide. What is not yet understood is how framework nacre proteins control crystal growth. Recently, a 30 AA intrinsically disordered nacre ...

PubMed

50
An acidic matrix protein, Pif, is a key macromolecule for nacre formation.
2009-08-13

The mollusk shell is a hard tissue consisting of calcium carbonate crystals and an organic matrix. The nacre of the shell is characterized by a stacked compartment structure with a uniformly oriented c axis of aragonite crystals in each compartment. Using a calcium carbonate-binding assay, we identified an acidic matrix protein, Pif, in the pearl oyster ...

PubMed

51
Molecular characterization and expression analysis of a heat shock protein 90 gene from disk abalone (Haliotis discus).
2010-02-04

Heat shock protein 90s (hsp90s) are chaperones that contribute to the proper folding of cellular proteins and help animals cope with the cellular protein damages in stress conditions. In this study, an hsp90 gene was isolated from disc abalone (Haliotis discus). The complete nucleotide sequence of the hsp90 gene ...

PubMed

52
Spiral and target patterns in bivalve nacre manifest a natural excitable medium from layer growth of a biological liquid crystal.
2009-06-15

Nacre is an exquisitely structured biocomposite of the calcium carbonate mineral aragonite with small amounts of proteins and the polysaccharide chitin. For many years, it has been the subject of research, not just because of its beauty, but also to discover how nature can produce such a superior product with excellent mechanical properties from such ...

PubMed

53
Spiral and target patterns in bivalve nacre manifest a natural excitable medium from layer growth of a biological liquid crystal
2009-06-30

Nacre is an exquisitely structured biocomposite of the calcium carbonate mineral aragonite with small amounts of proteins and the polysaccharide chitin. For many years, it has been the subject of research, not just because of its beauty, but also to discover how nature can produce such a superior product with excellent mechanical properties from such ...

PubMed Central

54
Macromolecular structure of the organic framework of nacre in Haliotis rufescens: implications for growth and mechanical behavior.
2008-04-25

We have performed a macromolecular structural analysis of the interlamellar and intertabular parts of the organic framework of the nacreous part of the shell of Haliotis rufescens, including the identification of structural chitin. Using histochemical optical microscopy we have mapped the locations of carboxylates and sulfates of proteins and chitin on the surfaces and within ...

PubMed

55
Amorphous layer around aragonite platelets in nacre
2005-09-06

We reveal that the aragonite CaCO3 platelets in nacre of Haliotis laevigata are covered with a continuous layer of disordered amorphous CaCO3 and that there is no protein interaction with this layer. This finding contradicts classical paradigms of biomineralization, e.g., an epitaxial match between the ...

PubMed Central

56
The nacre protein perlucin nucleates growth of calcium carbonate crystals.
2003-12-01

Atomic force microscopy (AFM) in aqueous solution was used to investigate native nacre of the marine snail Haliotis laevigata on the microscopic scale and the interaction of purified nacre proteins with calcium carbonate crystals on the nanoscopic scale. These investigations were controlled by scanning electron microscopy (SEM), light ...

PubMed

57
Molecular mechanistic origin of the toughness of natural adhesives, fibres and composites
1999-06-01

Natural materials are renowned for their strength and toughness,,,,. Spider dragline silk has a breakage energy per unit weight two orders of magnitude greater than high tensile steel,, and is representative of many other strong natural fibres,,. The abalone shell, a composite of calcium carbonate plates sandwiched between organic material, is 3,000 times more fracture ...

NASA Astrophysics Data System (ADS)

58
Physical methods in nanoscale science with the atomic force microscope
1998-12-01

The atomic force microscope (AFM) has opened up a wide gate to the nanoscopic world. Since its invention twelve years ago, it has allowed researchers to advance to new science. The extent of this advancement is strongly coupled to the sophistication of AFM instrumentation and to the methods with which AFMs are used. New AFMs and methods are needed to push the limits. Chapter 1 and 2 introduce such ...

NASA Astrophysics Data System (ADS)

59
Proteomic analysis of the acid-soluble nacre matrix of the bivalve Unio pictorum: detection of novel carbonic anhydrase and putative protease inhibitor proteins.
2010-10-18

The matrix extracted from mollusc shell nacre is a mixture of proteins and glycoproteins that is thought to play a major role in controlling biomineral synthesis and in increasing its mechanical properties. We investigated the nacreous shell of the freshwater mussel Unio pictorum, to which we applied a proteomics approach adapted to mollusc shell ...

PubMed

60
Covalent modification of chitin with silk-derivatives acts as an amphiphilic self-organizing template in nacre biomineralisation.
2009-05-04

Molluscs have a well-deserved reputation for being expert mineralizers of various shell types such as nacre. Nacre is defined as regularly arranged layers and stacks of approximately 0.5 microm thick aragonite platelets that are extracellularly formed within a complex mixture of organic matrix. The control of species-specific layer thickness by the animal ...

PubMed

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61
Nautilin-63, a novel acidic glycoprotein from the shell nacre of Nautilus?macromphalus.
2011-04-19

In molluscs, and more generally in metazoan organisms, the production of a calcified skeleton is a complex molecular process that is regulated by the secretion of an extracellular organic matrix. This matrix constitutes a cohesive and functional macromolecular assemblage, containing mainly proteins, glycoproteins and polysaccharides that, together, control the biomineral ...

PubMed

62
Spectral confocal reflection microscopy using a white light source
2008-08-01

We present a reflection confocal microscope incorporating a white light supercontinuum source and spectral detection. The microscope provides images resolved spatially in three-dimensions, in addition to spectral resolution covering the wavelength range 450-650nm. Images and reflection spectra of artificial and natural specimens are presented, showing features that are not normally revealed in ...

NASA Astrophysics Data System (ADS)

63
New toughening concepts for ceramic composites from rigid natural materials.
2010-08-07

The mechanisms underlying the toughening in rigid natural composites exhibited by the concentric cylindrical composites of spicules of hexactinellid sponges, and by the nacre (brick-and-mortar) structure of mollusks such as Haliotis rufescens (red abalone), as well as the crossed-lamellar structure of Strombus gigas (queen conch) show commonalities in the ...

PubMed

64
Atomic force microscope for small cantilevers
1997-04-01

We have designed and built an atomic force microscope (AFM) with optical beam deflection detection providing a focused spot size of 1.6 micrometers in diameter. This small spot size was implemented with a variable focus adjustment that allows us to re-focus on each cantilever. This design opens up the usage of a new range of small cantilevers with low-noise characteristics. We have microfabricated ...

NASA Astrophysics Data System (ADS)

65
Puget Sound Abalone - abalone

... in Washington's inside marine waters. Polygons in Puget Sound showing locations of Abalone, WDFW, 1991....

NBII National Biological Information Infrastructure

66
Study on the interaction between human serum albumin and a novel bioactive acridine derivative using optical spectroscopy.
2010-03-22

The interaction of a novel bioactive agent N-{[N-(2-dimethylamino) ethyl] acridine-4-carboxamide}-?-alanine [N-(ACR-4-CA)-?-ALA] with human serum albumin (HSA) was investigated by fluorescence spectroscopy, UV-vis absorption and circular dichroism spectrophotometric techniques under simulative physiological conditions. The fluorescence quenching of HSA by addition of ...

PubMed

67
Fracture Toughness of Nacre
2005-03-01

Nacre exhibits phenomenal fracture strength and toughness properties despite the brittle nature of its constituents. For example, its work of fracture is three orders of magnitude greater than that of a single crystal of its constituent mineral. This is surprising because it is a ceramic composite made up of about 95% brittle inorganic phase (aragonite mineral) and only a few ...

NASA Astrophysics Data System (ADS)

68
ZP Domain Proteins in the Abalone Egg Coat Include a Paralog of VERL under Positive Selection That Binds Lysin and 18-kDa Sperm Proteins
2010-01-18

Identifying fertilization molecules is key to our understanding of reproductive biology, yet only a few examples of interacting sperm and egg proteins are known. One of the best characterized comes from the invertebrate archeogastropod abalone (Haliotis spp.), where sperm lysin mediates passage through the protective egg vitelline envelope (VE) by binding ...

PubMed Central

69
Synchrotron X-ray Microbeam Diffraction from Abalone Shell
2004-03-01

Microstructured biomaterials such as mollusk shells receive much attention, due to the promise that advanced materials can be designed and synthesized with biomimetic techniques that take advantage of self-assembly and aqueous, ambient processing conditions. A satisfactory understanding of this process requires characterization of the microstructure at the growth fronts where control over crystal ...

NASA Astrophysics Data System (ADS)

70
Forming nacreous layer of the shells of the bivalves Atrina rigida and Pinctada margaritifera: an environmental- and cryo-scanning electron microscopy study.
2008-01-26

A key to understanding control over mineral formation in mollusk shells is the microenvironment inside the pre-formed 3-dimensional organic matrix framework where mineral forms. Much of what is known about nacre formation is from observations of the mature tissue. Although these studies have elucidated several important aspects of this process, the structure of the organic ...

PubMed

71
N40, a novel nonacidic matrix protein from pearl oyster nacre, facilitates nucleation of aragonite in vitro.
2007-10-12

A novel nonacidic matrix protein from pearl oyster nacre has been purified by cation-exchange chromatography. It was designated N40 for the nacreous protein of approximately 40 kDa. On the basis of the extraction method (with Tris-buffered Milli-Q water) and amino acid compositions (Gly- and Ala-rich), N40 was inferred to be a ...

PubMed

72
Parallel evolution of nacre building gene sets in molluscs.
2009-11-13

The capacity to biomineralize is closely linked to the rapid expansion of animal life during the early Cambrian, with many skeletonized phyla first appearing in the fossil record at this time. The appearance of disparate molluscan forms during this period leaves open the possibility that shells evolved independently and in parallel in at least some groups. To test this proposition and gain insight ...

PubMed

73
Identification, Characterization, and Expression of the Genes TektinA1 and Axonemal Protein 66.0 in the Tropical ...

... abalone species, H. asinina provides the highest relative meat weight (85% of the total weight), compared with ... template for nested PCR. The amplicons were geleluted, cloned, and sequenced. Nucleotide ...

NBII National Biological Information Infrastructure

74
Effects of traditional Chinese medicine on immune responses in abalone, Haliotis discus hannai Ino.
2008-02-14

A traditional Chinese medicine (TCM) preparation was formulated from orange peel (Pericarpium Citri Reticulatae), hawthorn (Crataegus pinnatifida), astragalus (Astragalus membranaceus (Fisch.) Bunge), pilose asiabell root (Radix codonopsis), indigowoad root (Radix isatidis), taraxacum (Herba taraxaci) and malt (Fructus Hordei Germinatus) at a weight ratio of 1:1:1.5:1.5:1.5:1.5:2. A feeding ...

PubMed

75
Black Abalone (Haliotis cracherodii)

... Photo: NOAABlack AbaloneHaliotis cracherodii Did You Know? Black abalone is one of 7 species of abalone found in California.· (click for larger image)Fi...

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76
Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell
2010-11-04

BackgroundThe formation of the molluscan shell is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell forming tissue, the mantle. This so called "calcifying matrix" is a complex mixture of proteins and glycoproteins that is assembled and occluded within the mineral phase during the calcification process. While the importance ...

PubMed Central

77
Abalone visceral extract inhibit tumor growth and metastasis by modulating Cox-2 levels and CD8+ T cell activity
2010-10-20

BackgroundAbalone has long been used as a valuable food source in East Asian countries. Although the nutritional importance of abalone has been reported through in vitro and in vivo studies, there is little evidence about the potential anti-tumor effects of abalone visceral extract. The aim of the present study is to examine anti-tumor ...

PubMed Central

78
Gene expression patterns of abalone, Haliotis tuberculata, during successive infections by the pathogen Vibrio harveyi.
2010-08-06

Since 1998, episodic mass mortality of the abalone Haliotistuberculata has been observed along the northern Brittany coast of France caused by a complex interaction among the host, pathogen and environmental factors. In the present study, abalone were submitted to two successive infections with the pathogen Vibrioharveyi under controlled conditions. During ...

PubMed

79
Astronomy & Astrophysics manuscript no. 14329 c ESO 2010 June 28, 2010

to the NACRE rate, the new rate is enhanced by up to 20 orders-magnitude. Aims. To validate this new rate, we to the rate provided in the NACRE (Angulo et al., 1999) compila- tion. Ogata et al. (2009) give the new rate 3OKK as a tabulated correction 3 to be applied to the rate of NACRE 3NACRE: log10(3OKK) = ...

E-print Network

80
A neurotropic herpesvirus infecting the gastropod, abalone, shares ancestry with oyster herpesvirus and a herpesvirus associated with the amphioxus genome
2010-11-10

BackgroundWith the exception of the oyster herpesvirus OsHV-1, all herpesviruses characterized thus far infect only vertebrates. Some cause neurological disease in their hosts, while others replicate or become latent in neurological tissues. Recently a new herpesvirus causing ganglioneuritis in abalone, a gastropod, was discovered. Molecular analysis of new herpesviruses, such ...

PubMed Central

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81
Identification and functional characterization of a putative 17?-hydroxysteroid dehydrogenase 12 in abalone (Haliotis diversicolor supertexta).
2011-04-09

The 17?-hydroxysteroid dehydrogenases (17?-HSDs) are key enzymes in the downstream process of steroid hormone biosynthesis. To date, relatively little is known about the role of 17?-HSDs in marine gastropods. In the present study, a putative cDNA sequence encoding type 12 17?-HSD (17?-HSD-12) was identified in abalone (Haliotis diversicolor supertexta). The full-length cDNA ...

PubMed

82
AP7, a partially disordered pseudo C-RING protein, is capable of forming stabilized aragonite in vitro.
2009-02-17

AP7 is an extracellular aragonite-associated protein of the nacre layer of the mollusk Haliotis rufescens and possesses a 36-amino acid C-terminal domain that exhibits sequence homology to the C subclass of the RING domain intracellular protein family. We report here novel findings which implicate AP7 as a member of the intrinsically ...

PubMed

83
Iridescence of a shell of mollusk Haliotis Glabra
2004-10-01

Pearls and shells of some mollusks are attractive inorganic materials primarily owing to the beauty of their natural lustrous and iridescent surface. The iridescent colors can be explained by diffraction or interference or both, depending on the microstructure of the surface. Strong iridescent colors are very evident on the polished shell of the mollusk Haliotis Glabra, commonly known as ...

NASA Astrophysics Data System (ADS)

84
White Abalone (Haliotis sorenseni)

White Abalone (Haliotis sorenseni) Kingdom: Animalia Class: Gastropoda Order: Archaeogastropoda Family: ...

NBII National Biological Information Infrastructure

85
Species of Concern Pink abalone

Species of Concern NOAA National Marine Fisheries Service Pink abalone Haliotis corrugata

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86
Species of Concern Green abalone

Species of Concern NOAA National Marine Fisheries Service Green abalone Haliotis fulgens

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87
Species of Concern Green abalone

... Species of Concern NOAA National Marine Fisheries Service Green abalone Haliotis fulgens ...

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88
Molecular cloning and expression of interleukin-1 receptor-associated kinase 4, an important mediator of Toll-like receptor signal pathway, from small abalone Haliotis diversicolor.
2011-03-17

Mammal interleukin-1 receptor-associated kinases (IRAKs) have been demonstrated to play important functions in TLRs (Toll-like receptor) signal pathway and T cell proliferation, but there is less knowledge available on mollusc IRAKs. In this study, a molluscan IRAK-4 gene, saIRAK-4, was cloned for the first time from the small abalone (Haliotis diversicolor). Its full-length ...

PubMed

89
Final critical habitat for Orange-nacre Mucket (Lampsilis perovalis)

This Polygon shapefile of critical habitat for the Orange-nacre Mucket (Lampsilis perovalis) based on the description ... ...

NBII National Biological Information Infrastructure

90
Responses of abalone Haliotis diversicolor to sublethal exposure of waterborne and dietary silver and cadmium.
2010-06-08

In this study, we examined the chronic waterborne and dietary exposure of silver (Ag) and cadmium (Cd) to the abalone Haliotis diversicolor using various endpoints such as growth and feeding rates, metal body burden, subcellular distribution, and metallothionein (MT) concentration over a period of 7 weeks of exposure. The growth and feeding rates of ...

PubMed

91
Nanomechanics of nanocomposite systems: Experimental studies in bone, seashells and synthetic nanocomposites using nanoindentation and atomic force microscopy
2008-01-01

Since last couple of decades, scientists and engineers are turning towards nature for material design lessons. Considerable efforts have been made to decode the design rules found in natural biological materials, which can be used to develop next-generation nanocomposite materials. The reason for these efforts lies in the extraordinary properties of biological materials that are not found in ...

NASA Astrophysics Data System (ADS)

92
Innate immune parameters and haemolymph protein expression profile to evaluate the immunotoxicity of tributyltin on abalone (Haliotis diversicolor supertexta).
2010-05-26

The immunotoxicity of tributyltin (TBT) on marine gastropods has been comparatively little studied although risks to wildlife associated with this compound are well known. In this study, a 30-day trial was conducted to evaluate the immunotoxic effects on abalone (Haliotis diversicolor supertexta) by exposing a range of doses of TBT (0, 2, 10, and 50 ng/L). Innate immune ...

PubMed

93
Microarray analysis of gene expression in disk abalone Haliotis discus discus after bacterial challenge.
2011-01-06

In this study, we investigated the gene expression profiling of disk abalone, Haliotis discus discus challenged by a mixture of three pathogenic bacteria Vibrio alginolyticus, Vibrio parahemolyticus, and Listeria monocytogenes using a cDNA microarray. Upon bacteria challenge, 68 (1.6%) and 112 (2.7%) gene transcripts changed their expression levels ?2 or ?2 -fold in gills and ...

PubMed

94
Materials become insensitive to flaws at nanoscale: Lessons from nature
2003-05-13

Natural materials such as bone, tooth, and nacre are nanocomposites of proteins and minerals with superior strength. Why is the nanometer scale so important to such materials? Can we learn from this to produce superior nanomaterials in the laboratory? These questions motivate the present study where we show that the nanocomposites in nature exhibit a ...

PubMed Central

95
Functional annotation of an expressed sequence tag library from Haliotis diversicolor and analysis of its plant-like sequences.
2011-06-25

The small abalone, Haliotis diversicolor, is a widely distributed and cultured species in the subtropical coastal area of China. To identify and classify functional genes of this important species, a normalized expressed sequence tag (EST) library, including 7069 high quality ESTs from the total body of H. diversicolor, was analyzed. A total of 4781 unigenes were assembled and ...

PubMed

96
Dietary phosphorus requirement of young abalone Haliotis discus Hannai Ino
2002-03-01

An experiment was performed to determine the dietary phosphorus requirement of the young abalone, Haliotis discus hannai. Five semi-purified diets were formulated to provide a series of graded levels of dietary total phosphorus (0.23% 1.98) from monobasic potassium phosphate (KH2P04). The brown alga, Laminaria japonica, was used as a control diet. Similar size ...

NASA Astrophysics Data System (ADS)

97
Nacre calcification in the freshwater mussel Unio pictorum: carbonic anhydrase activity and purification of a 95 kDa calcium-binding glycoprotein.
2008-10-13

The formation of the molluscan shell is finely tuned by macromolecules of the shell organic matrix. Previous results have shown that the acid-soluble fraction of the nacre matrix of the freshwater paleoheterodont bivalve Unio pictorum shell displays a number of remarkable properties, such as calcium-binding activity, the presence of extensive glycosylations and the capacity to ...

PubMed

98
Molecular characterization and gene expression of ferritin in red abalone (Haliotis rufescens).
2010-10-01

Ferritin is the principal iron storage protein in the majority of living organisms. Its capacity to capture the toxic cellular iron in excess in a compact and safe manner, gives to this protein a key role in detoxification and iron storage. It has a main role in cellular homeostasis and in cellular defense against oxidative stress produced by the reactive ...

PubMed

99
Prismin: A New Matrix Protein Family in the Japanese Pearl Oyster (Pinctada fucata) Involved in Prismatic Layer ...

... 1997) Modification of calcite crystal growth by abalone shell proteins: an atomic force microscope study. Biophys J 72: 1425�1433 CrossRef, PubMed, CSAWang N, Lee YH, Lee J (2008) Recombinant perlucin nucl...

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100
First molluscan transcription factor activator protein-1 (Ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury.
2010-08-21

The regulation of transcriptional activation is an essential and critical point in gene expression. In this study, we describe a novel transcription factor activator protein-1 (Ap-1) gene from disk abalone Haliotis discus discus (AbAp-1) for the first time in mollusk. It was identified by homology screening of an abalone normalized ...

PubMed

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101
� 1999 Macmillan Magazines Ltd the material. When we reach SmS, the state immediately above the

, is thought to hold the key to nacre's fracture toughness10,11 . Cera- mics laminated with organic material to nacre13 . We believe that the key to nacre's fracture resistance resides in the polymer adhesive the organic molecules exposed on the surface of freshly cleaved nacre. The adhesive �bres elongate

E-print Network

102
Nacre: An orthotropic and bimodular elastic material K. Bertoldi a

Nacre: An orthotropic and bimodular elastic material K. Bertoldi a , D. Bigoni b,*, W.J. Drugan b December 2007 Abstract A new micromechanical model for nacre (mother-of-pearl) is proposed, based and fracture are excluded), it is shown via homogenization techniques that the mechanical behaviour of nacre is

E-print Network

103
Multiscale structure of sheet nacre Marthe Rousseaua

Multiscale structure of sheet nacre Marthe Rousseaua , Evelyne Lopeza , Philippe Stempfl�b, c Ferollerie, 45071 Orl�ans cedex 2, France Abstract This work was conducted on Pinctada maxima nacre (mother bridges were evidenced by SEM in the interlaminar sequence. Keywords: Nacre; Aragonite; Nanocomposite

E-print Network

104
Mechanical properties of the elemental nanocomponents of nacre structure

Mechanical properties of the elemental nanocomponents of nacre structure Ph. Stempfl� a) * , O) Universit� d'Orl�ans, CNRS ISTO, 1A Rue de la F�rollerie, F-45071 Orl�ans cedex 2, France Sheet nacre? How does this nanostructure control the mechanical behaviour of sheet nacre at the macroscale

E-print Network

105
Department of Mechanical Engineering RESEARCH PROJECT

combinations of stiffness, strength and toughness. What can we learn from them? Seashells: nacre Teeth: enamel In-situ testing: fracturing nacre under the microscope Modeling crack bridging in nacre The hierarchical structure of nacre #12;Department of Mechanical Engineering OVERVIEW � Combustion and chemical

E-print Network

106
Nanoscale structural and mechanical characterization of natural nanocomposites: Seashells
2007-03-01

Seashells are natural nanocomposites with superior mechanical strength and toughness. What is the secret recipe that Mother Nature uses to fabricate seashells? What roles do the nanoscale structures play in the inelasticity and toughening of seashells? Can we learn from this to produce seashell-like nanocomposites? The focus of this article is nacre (mother-of-pearl). The ...

NASA Astrophysics Data System (ADS)

107
Microstructure observation and mechanical behavior modeling for limnetic nacre
2008-02-01

In the present research, microstructure of a kind of limnetic shell ( Hyriopsis cumingii) is observed and measured by using the scanning electron microscopy, and mechanical behavior experiments of the shell nacre are carried out by using bending and tensile tests. The dependence of mechanical properties of the shell nacre on its microstructure is analyzed ...

NASA Astrophysics Data System (ADS)

108
White Abalone Recovery Plan (2008)

... with the historical population center located at the California Channel Islands. Adult white abalone may be found between 5- ... Status and trends of white abalone at the Calif...

NBII National Biological Information Infrastructure

109
White Abalone (Haliotis sorenseni)

... EffortsRegulatory OverviewKey DocumentsMore Info Photo: John Butler, NOAAWhite Abalone(Haliotis sorenseni) Did You Know? The white abalone was the fi...

NBII National Biological Information Infrastructure

110
DEVELOPMENT AND CURRENT STATUS OF ABALONE AQUACULTURE IN CHILE

... only female abalone in the sea in the III region. With the growth in investment in abalone culture ... from 12°C to 21°C in the III region, 10°C to 19°C in the V ... ...

NBII National Biological Information Infrastructure

111
Mechanical properties and structure of Haliotis discus hannai Ino and Hemifusus tuba conch shells: a comparative study
2010-03-01

Haliotis discus hannai Ino (abalone shell) and Hemifusus tuba conch shell have been studied for the purpose to comparatively investigate the mechanisms by which nature designs composites. It is shown that both shells are composed of aragonite and a small amount of proteins while the conch shell shows finer microstructure but lower strength than ...

NASA Astrophysics Data System (ADS)

112
Sufficient Criteria for Applicability and Non-Applicability of Rule Sequences

transformation G (r2,m2) = H2 with NACr2 is asymmetrically parallel dependent on G (r1,m1) = H1 with NACr1 if: (i21 = m2, but e1 h21 |= NACr2 (produce-forbid-conflict). A direct transformation G (r2,m2) = H2 with NACr2 is asymmetrically parallel inde- pendent on G (r1,m1) = H1 with NACr1 if G ...

E-print Network

113
Architecture of Columnar Nacre, and Implications for Its Formation Mechanism
2007-06-29

We analyze the structure of Haliotis rufescens nacre, or mother-of-pearl, using synchrotron spectromicroscopy and x-ray absorption near-edge structure spectroscopy. We observe imaging contrast between adjacent individual nacre tablets, arising because different tablets have different crystal orientations with respect to the radiation's ...

Energy Citations Database

114
Architecture of Columnar Nacre, and Implications for Its Formation Mechanism
2007-06-01

We analyze the structure of Haliotis rufescens nacre, or mother-of-pearl, using synchrotron spectromicroscopy and x-ray absorption near-edge structure spectroscopy. We observe imaging contrast between adjacent individual nacre tablets, arising because different tablets have different crystal orientations with respect to the radiation�s polarization ...

NASA Astrophysics Data System (ADS)

115
Identification and expression localization of a Ran homologue in mollusc abalone, Haliotis diversicolor supertexta.
2011-01-11

Ran protein is a central molecular in several key nuclear functions, including nucleocytoplasmic transport, cell-cycle progression and nuclear envelope assembly. In this study, we have isolated and characterized a Ran homologue from a gastropod abalone which we named ab-Ran. The full-length cDNA consists of 1239 bp with an ORF encoding a 220 amino acid ...

PubMed

116
Transcriptome characterization of the South African abalone Haliotis midae using sequencing-by-synthesis
2011-03-11

BackgroundWorldwide, the genus Haliotis is represented by 56 extant species and several of these are commercially cultured. Among the six abalone species found in South Africa, Haliotis midae is the only aquacultured species. Despite its economic importance, genomic sequence resources for H. midae, and for abalone in general, are still scarce. Next ...

PubMed Central

117
LABORATORY OBSERVATIONS ON THE EARLY GROWTH OF THE ABALONE, HALIOTIS SORENSENI, AND THE EFFECT OF TEMPERATURE

LABORATORY OBSERVATIONS ON THE EARLY GROWTH OF THE ABALONE, HALIOTIS SORENSENI, AND THE EFFECT of temperature on larval development rate and growth of juveniles of the white or Sorensen's abalone, Haliotis growth of any of the seven American species of abalones exists in the literature. Details of larval

E-print Network

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