Table 1. Clinically relevant and atypical ABC proteins Kenneth J. Linton. Structure and Function of ABC Transporters. Physiology 22: 122-130, 2007

). #12;Chapter 1 2.2. Binding protein dependent ABC- type transporters The sugar transporters characterized so, and Rad50cd, a DNA repair protein which shows ATP-binding domains similar to the domains found in ABC-transporters a binding ...

of adeninenucleotide binding proteins is described in this chapter. Several overproduced ABC transporters of ABC (transport) proteins The use of the ATP analog BHacATP did identify multiple adenine of the family of ABC (transport) ...

-binding proteins (SBPs) of ATP Binding Cassette (ABC) transporters has been ongoing for at least four decades. Our on the lactococcal oligopeptide binding protein A (OppA), the receptor component of the ABC-transporter oligopeptide picture how these proteins work. Three ...

of SBP-dependent membrane proteins. A-B: visualizes ABC- transporters. A: ABC-importer with the SBP dependent transport proteins ABC-transporters exist in all three kingdom of life and transport a large of the membrane ...

such as glucose that are transported into the cell via binding protein dependent ABC transporters (Figure 3-binding protein of the glucose ABC transporter, were increased (Figure 5A/B). Possibly, this compensates in a severe defect of the cells to grow ...

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 6. Recognition of the transported solute by the ABC protein as typical ABC proteins that clearly serve other functions than transport, ...

proteins (Table 6). Those predicted to be ABC transporters in Table 6 could form new types of ABC of isolated ATP-binding protein components of ABC transporters by searching for conserved pairs of a membrane sides. #12;Table 6. Predicted Membrane ...

is a transmembrane domain that can couple to socalled energycoupling modules [38]. In ABC transporters, extra. It is a main source of metabolic energy in living cells, fuelling transport (via transport ATPases binding cassette (ABC) transport ATPases has been the focus of ...

- binding protein (or solute receptor) for function (Van der Heide & Poolman, 2002). ABC transporters for a more precise annotation of binding- protein-dependent ABC transporters in available genomes protein- dependent ABC ...

-binding-cassette transporter 1 (ABC1) has been implicated in processes related to membrane-lipid turnover. Here, using in vivo, as demonstrated by the lack of expression of ABC1 mRNA and protein (Fig. 1c, d). Analysis of heterozygous-phagocytic HeLa cell line with full-length ABC1 and with ...

fibrosis transmembrane conductance regulator (CFTR) that transports chloride ions [2]. In Escherischia coli at an interface [21]. The functional unit of ABC transporters is two transmembrane domains (TMD) complexed, et al., Repacking of the transmembrane domains of P- glycoprotein during the transport ATPase cycle

is then transported to the outer leaflet of the OM by seven recently discovered Lpt proteins, which are essential, is proposed to perform this function. Although ABC transporters have received considerable attention as targets for cancer chemotherapy, bacterial ABC ...

function, ABC transporters are composed of two hydrophobic transmembrane domains (TMDs) and two #Both multidrug transporter. #12;Chapter 2 12 Transmembrane domains The TMDs are two highly hydrophobic proteins transporter mutants that function in the absence of SBPs (see section `Transmembrane ...

The ATP binding cassette (ABC) transporters, or traffic ATPases, constitute a large family of proteins responsible for the transport of a wide variety of substrates across cell membranes in both prokaryotic and eukaryotic cells. We describe a human ABC protein with regions ...

One of the major problems related with anticancer chemotherapy is resistance against anticancer drugs. The ATP-binding cassette (ABC) transporters are a family of transporter proteins that are responsible for drug resistance and a low bioavailability of drugs by pumping a variety of drugs out cells at the expense ...

]iodoarylazidoprazosin Mammalian ABC (ATP-binding cassette) transporter proteins are important for a variety of cell processes resistance and cancer: The role of the human ABC transporter ABCG2. Curr. Protein Pept. Sci. 3: 503 members of the ATP-binding cassette ...

) are consistent with a binding protein-dependent ATP binding cassette (ABC) transporter. A corresponding binding. acidocaldarius as a solute binding protein component of an ATP binding cassette (ABC) transport system for mal membranes (27). Typically, an ...

of selenomethion- ine in the membrane protein complex OpuA (an ABC transporter) and the soluble protein OppA, both for overexpression of complex, multisubunit proteins (such as ABC and TRAP transporters) and mammalian membrane ...

The family of ATP binding cassette (ABC) transporters or traffic ATPases is composed of several membrane-associated proteins that transport a great variety of solutes across cellular membranes. Two novel mammalian members of the family, ABC1 and ABC2, have been identified ...

The ABC1 (ABCA) subfamily of the ATP-binding cassette (ABC) transporter superfamily has a structural feature that distinguishes it from other ABC transporters. Here we report the cloning, molecular characterization and tissue distribution of ABC2/ABCA2, which belongs to the ...

of about 130 residues. This extension is also found in many binding protein- dependent ABC transporters calorimetry and trypsin digestion. GlcV is a subunit of a binding protein dependent ABC-type glucose transport Domain of the Glucose ABC ...

The ABC gene family code for transporter proteins pumping a variety of compounds across the membranes of cells and tissues. Overexpression of ABC transporters is an important cause of multidrug resistance to chemotherapy agents and these genes are mutated in diverse human diseases.

Access to the article is free, however registration and sign-in are required. The ABC transporters are transmembrane proteins that move a wide variety of compounds into and out of cells. In her Perspective, Davidson explains what the structure of the second bacterial ABC transporter to be ...

cassette (ABC) transporter family have been implicated in both antibiotic and cancer-drug resistance. The mechanisms used by these proteins to expel toxins from cells therefore...

BackgroundThe large gene superfamily of ABC (ATP-binding cassette) transporters encodes membrane proteins involved in trafficking processes across biological membranes and further essential cell biological functions. ABC transporters are evolutionary ancient and involved in the biochemical ...

by the Sec or the Tat pathways. Finally, ABC transporters, the Sec or Tat pathways secrete proteins knowledge of protein transport pathways and their (putative) substrates (Antelmann et al., 2001; Tjalsma et of protein transport across membranes, as ...

This paper reports the characterization of a new ABC transporter (LtrABC1.1), related to the human ABCA subfamily, in the protozoan parasite Leishmania tropica. LtrABC1.1 is a tandem duplicated gene flanked by inverted repeats. LtrABC1.1 is expressed mainly in the flagellar pocket of the ...

CFTR chloride channels are encoded by the gene mutated in patients with cystic fibrosis. These channels belong to the superfamily of ABC transporter ATPases. ATP-driven conformational changes, which in other ABC proteins fuel uphill substrate transport across cellular membranes, in CFTR open ...

CFTR chloride channels are encoded by the gene mutated in patients with cystic fibrosis. These channels belong to the superfamily of ABC transporter ATPases. ATP-driven conformational changes, which in other ABC proteins fuel uphill substrate transport across cellular membranes, in CFTR open ...

CFTR chloride channels are encoded by the gene mutated in patients with cystic fibrosis. These channels belong to the superfamily of ABC transporter ATPases. ATP-driven conformational changes, which in other ABC proteins fuel uphill substrate transport across cellular membranes, in CFTR open ...

named OpuAC, belongs to a large superfamily of proteins associated with ABC transporters involved-Jan Slotboom (*) Shared first authorship 4.1 Abstract T HE ABC transporter OpuA from Lactococcus lactis The osmoregulatory ABC transporter OpuA protects ...

and trehalose. Uptake of -glucosides is mediated by two different binding protein- dependent ABC-type transportChapter 3 1 Biochemical evidence for the presence of two - glucoside ABC-transport systems via ATP-binding cassette (ABC) transporters (Ehrmann et al., 1998). In ...

proteins involved in lipid transport. Transmembrane domains (TMDs) are shown as membrane-spanning helicesReview Function of prokaryotic and eukaryotic ABC proteins in lipid transport Antje Pohla of both eukaryotic and prokaryotic origins are implicated in the transport of ...

Cells tolerate exposure to cytotoxic compounds through the action of ATP-driven efflux pumps belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters. Phytopathogenic fungi encounter toxic environments during plant invasion as a result of the plant defense response. Here we demonstrate the requirement for an ABC ...

BackgroundThe superfamily of ABC proteins is among the largest known in nature. Its members are mainly, but not exclusively, involved in the transport of a broad range of substrates across biological membranes. Many contribute to multidrug resistance in microbial pathogens and cancer cells. The diversity of ABC ...

This predoctoral training grant supported by the Department of Defense Breast Cancer Research Program (BCRP) of the Office of the Congressionally Directed Medical Research Programs (CDMRP) aims to study the possible role of ABC transporters in altered apo...

This predoctoral training grant, supported by the Department of Defense Breast Cancer Research Program (BCRP) of the Office of the Congressionally Directed Medical Research Programs (CDMRP), aims to study the possible role of ABC transporters in pleitropi...

ATP binding cassette (ABC) transporters define a family of proteins with strong structural similarities conserved across evolution and devoted to the translocation of a variety of substrates across cell membranes. A few members of the family are known in mammals, but although all of them are medically relevant ...

ATP binding cassette (ABC) transporters, which are found in all species, are known mainly for their ability to confer drug resistance. To date, most of the ABC transporters characterized in plants have been localized in the vacuolar membrane and are considered to be involved in the intracellular sequestration of ...

: the ATP-binding cassette (ABC) transporters and the natural resistance-associated macrophage proteins transport system for manganese, and psbO, encoding the extrinsic manganese stabilizing protein affinity manganese transport system different from the more thoroughly ...

, which is a member of the superfamily of ATP-binding-cassette (ABC) transport proteins (1). MalF and Mal in ABC transporters. A conserved sequence in hydrophobic membrane proteins of periplasmic permeases transport in vivo. In contrast, the ATPase ...

ATP-binding cassette (ABC) transporters form a large family of transmembrane proteins that facilitate the transport of specific substrates across membranes in an ATP-dependent manner. Transported substrates include lipids, lipopolysaccharides, amino acids, peptides, ...

The gene for this cyclophilin encodes a protein that contains a unique .... Selectivity of transport, with respect to ionic charge, is determined by the ... differences in extent and type of interaction between ABC personnel and various ...

Access to the article is free, however registration and sign-in are required. Proteins pump lipophilic molecules out of cells, establishing gradients that guide cell movement during development.

Some ABC transporters play a significant role in human health and illness because they confer multidrug resistance (MDR) through their overexpression. Compounds that inhibit the drug efflux mechanism can improve efficacy or reverse resistance. Of the eight described ABC transporter subfamilies, those ...

through high affinity binding of protein-dependent ABC transporters. We have investigated the expression regulators of the trehalose/maltose ABC transporters from P. furiosus and T. litoralis (Lee et al., 2003;Lee, 2001). In S. solfataricus transport of glucose and ...

The ATP-binding cassette (ABC) class of proteins is one of the most functionally diverse transporter families found in biological systems. Although the abundance of ABC proteins varies between species, they are highly conserved in sequence and often demonstrate similar functions across ...

We report that the genes abc, yaeC, and yaeE comprise metD, an Escherichia coli locus encoding a dl-methionine uptake system. MetD is an ABC transporter with Abc the ATPase, YaeE the permease, and YaeC the likely substrate binding protein. Expression of these genes is regulated by l-methionine ...

We report on the transcriptional patterns of a putative white (w) gene encoding an ABC-transporter protein during development in Hessian fly, Mayetiola destructor. The deduced amino acid sequence for the Hessian fly white showed 77 to 74% similarities to white/ATP-binding-cassette proteins and 57 t...

ABC transporters (also known as traffic ATPases) form a large family of proteins responsible for the translocation of a variety of compounds across membranes of both prokaryotes and eukaryotes. The recently completed Escherichia coli genome sequence revealed that the largest family of paralogous E. coli proteins is ...

Recently we described identification and characterization of GDSL esterase EstA from psychrotrophic bacterium Pseudoalteromonas sp. 643A. Attempts to obtain heterologous overexpression of this enzyme in Escherichia coli system were not satisfactory. The EstA protein was expressed as inclusion bodies, most of that were inactive after purification step, and the recovery of ...

We have used a fluorescence-based thermal shift (FTS) assay to identify amino acids that bind to solute-binding proteins in the bacterial ABC transporter family. The assay was validated with a set of six proteins with known binding specificity and was consistently able to map proteins with ...

Peroxisomes are indispensable organelles in higher eukaryotes. They are essential for a number of important metabolic pathways, including fatty acid ?- and ?-oxidation, and biosynthesis of etherphospholipids and bile acids. However, the peroxisomal membrane forms an impermeable barrier to these metabolites. Therefore, peroxisomes need specific transporter ...

Atherosclerotic cardiovascular disease is the leading cause of morbidity and mortality in the United States and in many other countries. Dysfunctional lipid homeostasis plays a central role in the initiation and progression of atherosclerotic lesions. The ATP-binding cassette (ABC) transporters are transmembrane proteins that hydrolyze ...

We recently cloned a full-length cDNA of the rat ATP-binding cassette transporter 2 (ABC2, or ABCA2) protein, a member of the ABC1 (or ABCA) subfamily (-ABC1/ABCA1 is a causal gene for Tangier disease) and found it to be strongly expressed in the rat brain. In this study, we identified ...

The ATP-binding cassette (ABC) transporters are one of the largest evolutionarily conserved families of proteins. They are characterized by the presence of nucleotide-binding domains (NBDs), which are highly conserved among organisms. In the present study, we used human and protozoan ABC sequences, and ATP-binding ...

An underlying mechanism for multi drug resistance (MDR) is up-regulation of the transmembrane ATP-binding cassette (ABC) transporter proteins. ABC transporters also determine the general fate and effect of pharmaceutical agents in the body. The three major types of ABC ...

: ____ Sec-dependent secretion systems transport unfolded substrates ____ a co-factor requiring protein ____ type I secretion mediated by TolC is dependent on an ABC transporter 2. Regarding membranes points) 36. Explain how vanadate can be used to assess ATP hydrolysis by an ABC ...

To examine whether the fission yeast Mam1 ABC transporter can be used for secretion of heterologous proteins, thereby bypassing the classical secretion pathway, we have analyzed chimeric forms of the M-factor precursor. It was demonstrated that GFP can be exported when fused to both the amino-terminal prosequence from mfm1 and a CaaX ...

J. Thomas and John F. Hunt The 4.5 � map of the MsbA protein, a putative lipid A transporter from `loops' of an ABC transporter and the geometric relationship of these regions to the ATP-binding cassette to the other. ATP-binding cas- sette (or ABC) transporters represent ...

) and the Tat system, which transports proteins folded in the cytoplasm (57). However, to our knowledge, allD, and LolE, which constitutes an ATP-binding cassette (ABC) transporter in the IM, is required and subsequent lipidation at the N-terminal cysteine (C), lipoproteins bind to the ...

Access to the article is free, however registration and sign-in are required. Cells produce a variety of ABC transporter proteins that shuttle numerous molecules across the plasma membrane so as to maintain the integrity of the intracellular milieu. As Higgins and Linton discuss in their Perspective, the first high-resolution crystal ...

MA3998 ABC transporter, ATP-binding protein R 59,883 5.43 129 ND MA4012 conserved hypothetical protein S 39,745 4.87 172 ND MA4406 ABC transporter, ATP-binding protein O 27,719 5 protein S 32,353 5.60 ND 56 MA0659 ...

Every cell is separated from its external environment by a lipid membrane. Survival depends on the regulated and selective transport of nutrients, waste products and regulatory molecules across these membranes, a process that is often mediated by integral membrane proteins. The largest and most diverse of these membrane transport ...

Studies have shown the fundamental contribution of the yeast vacuole as a site for storage and detoxification of metals. Whereas the transmembrane proteins responsible for iron transport into and out of the vacuole have been identified in Saccharomyces cerevisiae, less information is available concerning the mobilization of vacuolar iron stores in ...

Increased production of penicillin-binding protein PBP 4 is known to increase peptidoglycan cross-linking and contributes to methicillin resistance in Staphylococcus aureus. The pbp4 gene shares a 400-nucleotide intercistronic region with the divergently transcribed abcA gene, encoding an ATP-binding cassette transporter of unknown ...

Adenosine triphosphate-binding cassette (ABC) transporters, such as P-glycoprotein (Pgp, ABCB1), breast cancer resistance protein (BCRP, ABCG2) and multidrug resistance-associated proteins (MRPs) are expressed in high concentrations at various physiological barriers (e.g. blood-brain barrier, blood-testis barrier, ...

BackgroundThe spread of drug resistance has been a major obstacle to the control of malaria. The mechanisms underlying drug resistance in malaria seem to be complex and multigenic. The current literature on multiple drug resistance against anti-malarials has documented PfMDR1, an ATP-binding cassette (ABC) protein, as an important determinant of ...

Summary: Complex glycoconjugates play critical roles in the biology of microorganisms. Despite the remarkable diversity in glycan structures and the bacteria that produce them, conserved themes are evident in the biosynthesis-export pathways. One of the primary pathways involves representatives of the ATP-binding cassette (ABC) transporter superfamily. ...

ABC transporters have been implicated in a variety of human diseases. The ABCR gene and its protein have been linked to Stargardt's disease, fundus flavimaculatus, cone-rod dystrophy, retinitis pigmentosa, and age-related macular degeneration. The genetic and molecular pathways involved in the pathogenesis of ABCR-related ophthalmic ...

The essential trace compounds tungstate and molybdate are taken up by cells via ABC transporters. Despite their similar ionic radii and chemical properties, the WtpA protein selectively binds tungstate in the presence of molybdate. Using site-directed mutagenesis of conserved binding pocket residues, we established a molecular basis ...

Some ATP-binding cassette (ABC) transporters of subfamilies B, C and G confer resistance to xenobiotics including insecticides. We identified genes of these subfamilies expressed by the lepidopterans Trichoplusia ni and Bombyx mori. The B. mori genome includes eight, six and 13 ABC-B, -C and -G genes, respectively, which encode ...

-RACE primers were designed from the gene sequences of ATM1a (a mitochondrial ABC transporter), cysteine with the Saccharomyces cerevisiae ORF, YIL006W coding for an inner mitochondrial membrane protein transporting pyruvate targeted proteins in the spore. With the characterisation of IMP and ...

ATP-binding cassette (ABC) transporters are ubiquitous integral membrane proteins, which catalyze the translocation of molecules across biological membranes in an ATP-dependent manner. Despite the diversity in the transported substrates, they all share the same architecture, comprised of two transmembrane (TMD) and ...

There exist four fundamentally different classes of membrane-bound transport proteins: ion channels; transporters; aquaporins; and ATP-powered pumps. ATP-binding cassette (ABC) transporters are an example of ATP-dependent pumps. ABC transporters are ...

There exist four fundamentally different classes of membrane-bound transport proteins: ion channels; transporters; aquaporins; and ATP-powered pumps. ATP-binding cassette (ABC) transporters are an example of ATP-dependent pumps. ABC transporters are ...

Summary: ATP-binding cassette (ABC) systems are universally distributed among living organisms and function in many different aspects of bacterial physiology. ABC transporters are best known for their role in the import of essential nutrients and the export of toxic molecules, but they can also mediate the ...

In the present study, the gene expression of three multidrug resistance (MDR) and resistance-associated protein (MRP) transport proteins or efflux pumps was characterized and the phenotypic evidence for such pumps was demonstrated in cultured Madin-Darby canine kidney (MDCK) cells. A gradient for the fluorescent probe calcein was ...

and membrane proteins from different families, although ABC transporters that hold extracellular substrate-peptides, or by engineering the transport capacity for branched-chain amino acids, the expression levels of membrane proteins Introduction Membrane proteins perform ...

ATP-binding cassette (ABC) transporters belong to one of the largest protein families that either import or export a wide spectrum of different substrates. Certain members of this superfamily have been implicated in multidrug resistance in various types of cancer as well as in pathogenic microorganisms. The role of ...

In parasites, ATP-binding cassette (ABC) transporters represent an important family of proteins related to drug resistance and other biological activities. Here we report the characterization of LtrABCA2, a new ABC transporter of the ABCA subfamily in the protozoan parasite Leishmania tropica, ...