of selenomethion- ine in the membrane protein complex OpuA (an ABC transporter) and the soluble protein OppA, both for overexpression of complex, multisubunit proteins (such as ABC and TRAP transporters) and mammalian membrane transporter for glycine betaine ...

named OpuAC, belongs to a large superfamily of proteins associated with ABC transporters involved-Jan Slotboom (*) Shared first authorship 4.1 Abstract T HE ABC transporter OpuA from Lactococcus lactis The osmoregulatory ABC transporter ...

BackgroundThe ABC transporter OpuA from Lactococcus lactis transports glycine betaine upon activation by threshold values of ionic strength. In this study, the ligand binding characteristics of purified OpuA in a detergent-solubilized state and of its substrate-binding domain produced as ...

In the soil bacterium Bacillus subtilis, five transport systems work in concert to mediate the import of various compatible solutes that counteract the deleterious effects of increases in the osmolarity of the environment. Among these five systems, the ABC transporter OpuA, which catalyzes the import of glycine ...

We have previously shown that the C-terminal cystathionine ?-synthase (CBS) domains of the nucleotide-binding domains of the ABC transporter OpuA, in conjunction with an anionic membrane surface function, act as sensor of internal ionic strength (I_in). Here, we show that a surface-exposed cationic region in ...

The CBS module of OpuA in conjunction with an anionic membrane surface acts as sensor of internal ionic strength, which allows the protein to respond to osmotic stress. We now show by chemical modification and cross-linking studies that CBS2-CBS2 interface residues are critical for transport activity and/or ionic regulation of ...

Lactococcus lactis is a promising host for (membrane) protein overproduction. Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in L. lactis. Incorporation efficiencies of SeMet in the membrane protein complex OpuA (an ABC transporter) and the soluble protein OppA, both from L. lactis, ...

Table 1. Clinically relevant and atypical ABC proteins Kenneth J. Linton. Structure and Function of ABC Transporters. Physiology 22: 122-130, 2007

). #12;Chapter 1 2.2. Binding protein dependent ABC- type transporters The sugar transporters characterized so, and Rad50cd, a DNA repair protein which shows ATP-binding domains similar to the domains found in ABC-transporters a binding protein-dependent ATP- binding cassette (ABC)-transporter ...

-binding proteins (SBPs) of ATP Binding Cassette (ABC) transporters has been ongoing for at least four decades. Our on the lactococcal oligopeptide binding protein A (OppA), the receptor component of the ABC-transporter oligopeptide picture how these proteins work. Three types of ABC-transporters exist: ...

of SBP-dependent membrane proteins. A-B: visualizes ABC- transporters. A: ABC-importer with the SBP dependent transport proteins ABC-transporters exist in all three kingdom of life and transport a large of the membrane protein. In mGluR this signal is triggered by ligand ...

Etravirine is a novel nonnucleoside reverse transcriptase inhibitor (NNRTI) for the treatment of HIV-1 infections. ABC transporters potentially mediate clinically relevant drug-drug interactions. We assessed substrate characteristics and the inhibitory and inductive potential of etravirine on ABC transporters. ...

FIGURE 1. The minimal ABC transporter has four domains. Two transmembrane domains (TMDs) bind ligand, and transport is driven by ATP binding and hydrolysis by the two nucleotide binding domains (NBDs). The TMDs from different subfamilies of ABC transporters are not necessarily homologous. The ...