Antibodies Against Abnormal Glycoproteins Identified as Possible Biomarkers for Cancer Detection Scientists have found that cancer patients produce antibodies that target abnormal glycoproteins (proteins with sugar molecules attached) made by their tumors. The result of this work suggests that ...
Cancer.gov
The conserved oligomeric Golgi (COG) complex was identified as one of the evolutionary conserved protein complexes that regulate a cis-Golgi step in intracellular vesicular transport. This evolutionary conserved complex is composed of eight subunits. Muta...
National Technical Information Service (NTIS)
The conserved oligomeric Golgi (COG) complex composed of eight subunits was identified as one of the evolutionary conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. Available mutants with the mutations in COG...
... Title : Role of the Conserved Ologomeric Golgi Complex in the Abnormalities of Glycoprotein Processing in Breast Cancer Cells. ...
DTIC Science & Technology
... Title : Role of the Conserved Oligomeric Golgi Complex in the Abnormalities of Glycoprotein Processing in Breast Cancer Cells. ...
... Title : Role of Conserved Oligomeric Golgi Complex in the Abnormalities of Glycoprotein Processing in Breast Cancer Cells. ...
One reason that some people are prone to calcium oxalate nephrolithiasis is that they produce urine that is subnormal in its ability to inhibit the growth of calcium oxalate crystals. We have identified in human urine a glycoprotein (GCI) that inhibits calcium oxalate crystal growth strongly, and at low concentrations (10(-7) M); in this study, we have ...
PubMed Central
The fungal toxin fusicoccin induces plant wilting by affecting ion transport across the plasma membrane of plant cell. The activity of this toxin is so far unknown in humans. In the present study we show that fusicoccin is able to affect the platelet aggregation process. The toxin associates with platelet intracellular binding sites and induces aggregation in platelet-rich plasma in a ...
PubMed
Early studies have shown that spotted locoweed (Astragalus lentiginosus) has an adverse effect on male reproduction. Rams fed locoweed showed a reduced number of primary and secondary spermatocytes and spermatids in the testis, and of spermatozoa in the epididymis and vas deferens. In addition, the Sertoli cells and other epithelial cells were severely vacuolated. Swainsonine, an indolizidine ...
... sperm suspensions reveals that HEP64 forms a shroudlike coating surrounding abnormal spermatozoa. The HEP64 glycoprotein firmly binds ... immunocytochemistry demonstrates that HEP64 localized to an amorph...
NBII National Biological Information Infrastructure
The aims of this project are to isolate mucous glycoproteins from the tracheobronchial tract of pigs and humans, to identify other secretory products in respiratory secretions which may interact with mucous glycoprotein, and to determine the chemical, imm...
It is currently unclear whether the T cell dysfunctions observed during active systemic lupus erythematosus (SLE) reflect a disorder intrinsic to the T cell or defects that result from interaction with anti-T cell autoantibody. To determine whether a disorder intrinsic to the T cell exists in SLE, the T cell capping mechanism was selected as a model of cellular function. The normal T cell capping ...
The major envelope glycoprotein of the causative agent of Acquired Immune Deficiency Syndrome (AIDS) lymphadenopathy-associated virus (LAV) has been identified and characterized. The glycoprotein has an apparent molecular weight of 110,000-120,000 under denaturing conditions in polyacrylamide gel electrophoresis. Upon deglycosylation ...
Energy Citations Database
Trophoblastic cell fusion is one essential step of the human trophoblast differentiation leading to formation of the syncytiotrophoblast, site of the numerous placental functions. This process is multifactorial and finely regulated. Using the physiological model of primary culture of trophoblastic cells isolated from human placenta, we have identified different membrane ...
Con A acceptor glycoproteins were analyzed by 2D-PAGE and {sup 125}I-Con A overlay in three squamous carcinoma cell lines and compared with those in the simian virus (SV40)-transformed keratinocyte cell line SVK-14 and in normal keratinocytes. The majority of the glycoproteins identified by this technique were expressed at similar ...
The aims of the project are to isolate mucous glycoproteins (MGP) from the tracheobronchial tract of pigs and humans, to identify other products in respiratory secretions from these sources which may interact with MGP and to determine the chemical immunoc...
rare hereditary disorders are known to promote the formation of aneurysms: Marfan's and Ehlers-Danlos' Syndromes (Wilmink et al. 2000). Marfan's Syndrome causes an abnormal breakdown of the elastic fibers
E-print Network
Jul 16, 1999 ... NAD synthetase is target for structure based design of antibiotics. ... oligosaccharides, glycoproteins, or glycolipids; present as a surface antigen in myxoviruses. ... Perturbation: A perturbation is a disturbance or abnormality. ...
NASA Website
The conserved oligomeric Golgi (COG) complex consists of eight subunits that are thought to be involved in vesicle tethering. Available mutants with the mutations in COG complex subunits exhibit defects in basic Golgi functions: protein glycosylation and ...
A new technology that enables the detection of antibodies in the blood that target abnormal glycoproteins produced by cancer cells may make it possible to use these antibodies as biomarkers for the early detection of cancer.
We have been studying a family of proteins that we have termed SIBLINGs for Small Integrin Binding Llgand N-linked Glycoproteins that share similar structural domains human chromosomal location normal synthesis by skeletal tissue and abnormal expression b...
We have been studying a family of proteins that we have termed SIBLINGs for Small Integrin Binding Ligand N-linked Glycoproteins, that share similar structural domains, human chromosomal location, normal synthesis by skeletal tissue, and abnormal expressi...
We have been studying a family of proteins that we have termed SIBLINGs for Small Integrin Binding LIgand N-linked Glycoproteins, that share similar structural domains, human chromosomal location, normal synthesis by skeletal tissue, and abnormal expressi...
Karyotyping is a test used to identify chromosome abnormalities as the cause of malformation or disease. The ...
MedlinePLUS
... presented for a physical examination, ophthalmologic evaluation, and neurologic assessment. Abnormalities identified on physical examination included subjective ... ...
Trypanosoma cruzi, the agent of the American trypanosomiasis or Chagas disease, bypasses its lack of de novo synthesis of sialic acids by expressing a surface-anchored trans-sialidase. This enzyme transfers sialic acid residues from the host's sialylglycoconjugates to the parasite's galactosylglycoconjugates. In addition to carrying out a pivotal role in parasite persistence/replication within the ...
The Gray platelet syndrome (GPS) is a rare inherited disorder linked to undefined molecular abnormalities that prevent the formation and maturation of ?-granules. Here, we report studies on two patients from unrelated families that confirm phenotypic heterogeneity in the disease. First we used immunoelectron microscopy (I-EM) to confirm that TREM-like transcript-1 (TLT-1) is ...
This multi-disciplinary team of experts in glycomics, proteomics, informatics, and cancer markers focuses on the analysis and identification of glycoproteins in plasma and tissue and is developing analytical approaches to identify and characterize changes in the glycan associated with these glycoproteins. Candidate ...
A mannose-containing 40K glycoprotein has been identified in the stratum corneum of normal human epidermis. It is apparently membrane-bound and in the intact epidermis it is inaccessible to either concanavalin A or to trypsin. After it is detergent-solubilized, it can be labeled with concanavalin A or destroyed with trypsin. There is little or none of this ...
Two human cell lysosomal membrane glycoproteins of approx. 120-kDa, hLAMP-1 and hLAMP-2, were identified by use of monoclonal antibodies prepared against U937 myelomonocytic leukemia cells or blood mononuclear cells. The two glycoproteins were purified by...
Due to the critical roles of glycoproteins in the activities of cells to tissues, mapping of liver glycoproteome may provide valuable basic information for finding disease marker proteins. In this study, Datura Stramonium Agglutinin (DSA) was chosen to enrich N-linked glycoproteins for its broader specificity with tri- or tetra-antennary complex type. DSA ...
cytotechnician visually scans the microscopic images and identifies gross abnormal'ities such as rings, dicentrics, gaps, chromatid breaks, and chromosome ...
A robust, reproducible, and high throughput method was developed for the relative quantitative analysis of glycoprotein abundances in human serum. Instead of quantifying glycoproteins by glycopeptides in conventional quantitative glycoproteomics, glycoproteins were quantified by nonglycosylated peptides derived from the ...
... We have identified four regions of the human immunodeficiency virus type 1 HIV-1 envelope glycoprotein that have the capacity to induce ...
... phage display, bioinformatics, and virus neutralization assays, we identified two important regions (Epitope I and Epitope II) on the E2 envelope glycoprotein ...
Center for Biologics Evaluation and Research (CBER)
The advances in ultrasound technology have made it possible to identify fetal structural abnormalities and genetic syndromes in the first trimester. First trimester prenatal diagnosis of fetal central nervous system, renal, gastrointestinal, cardiac, and skeletal abnormalities is reviewed. PMID:11981912
Recent advancements in molecular developmental biology afford an opportunity to apply newly developed tools for understanding the mechanisms of both normal and abnormal development. lthough a number of agents have been identified as causing developmental abnormalities, knowledge ...
EPA Science Inventory
The total proteins and concanavalin A-binding glycoproteins of the cultivated mushroom Agrocybe aegerita were studied in homokaryotic siblings and in dikaryotic strains. The glycoproteins exhibited considerable variability compared with the proteins; the genetic diversity detected in homokaryons in the glycoprotein analysis was 30-fold ...
The effect of N-benzoyl-D-phenylalanine (NBDP) and metformin on neonatal streptozotocin (nSTZ) induced diabetes has been studied on plasma and tissue glycoproteins. In some pathological conditions, such as cancer, rheumatoid arthritis and diabetes, there is an abnormal glycosylation of acute phase serum proteins. As most serum proteins are produced in the ...
BackgroundThe mammalian otoconial membrane is a dense extracellular matrix containing bio-mineralized otoconia. This structure provides the mechanical stimulus necessary for hair cells of the vestibular maculae to respond to linear accelerations and gravity. In teleosts, Otolin is required for the proper anchoring of otolith crystals to the sensory maculae. Otoconia detachment and subsequent ...
Glycosyltransferases are involved in the biosynthesis of lipid-linked N-glycans. Here, we identify and characterize a mannosyltransferase gene from Arabidopsis thaliana, which is the functional homolog of the ALG3 (Dol-P-Man:Man5GlcNAc2-PP-Dol ?1,3-mannosyl transferase) gene in yeast. The At ALG3 protein can complement a ?alg3 ...
The influence of P-glycoprotein (ABCB1) in drug resistance as well as drug absorption and disposition is an important factor to be considered during the development of new drugs. Thus, the early identification and exclusion of compounds showing a high affinity towards P-glycoprotein can help to select drug candidates. The aim of our study was to implement ...
Pneumocystis carinii is an opportunistic pathogen of man, carried as a commensal in healthy subjects. It frequently causes a fatal pneumonia in the immunosuppressed host. It is a major complication of HIV-1 infection in man (AIDS). Using surface radioiodination of rat-derived P. carinii trophozoites obtained from in vitro culture, a major surface glycoprotein (gp120) has been ...
A cell surface glycoprotein antigen with an apparent molecular weight of about 100,000 that is selectively expressed on proliferating cells was purified from deoxycholate-solubilized membranes of a cultured human leukemic thymus-derived (T) cell line by affinity chromatography on a monoclonal antibody-Sepharose column. A conventional xenoantiserum prepared by immunization with ...
Fukuyama-type congenital muscular dystrophy (FCMD) is a severe form of muscular dystrophy accompanied by abnormalities in the eye and brain. The incidence of FCMD is particularly high in the Japanese population. Mutations in the fukutin gene have been identified in patients with FCMD. Fukutin is predicted to be a Golgi apparatus resident protein and to be ...
The Pop1/Bves (blood vessel/epicardial substance) gene is a member of the popeye gene family recently identified in various species. It encodes a potential transmembrane glycoprotein and is a cell adhesion molecule present in skeletal and cardiac muscle and epithelia. We isolated the Drosophila homologue of Bves (DmBves) and found, using in situ ...
The sulfated proteoglycans on the surface of cultured mammalian cells have been implicated in a variety of phenomena. To obtain more direct evidence for the role of these molecules in specific cellular functions, they are isolating mutants that produce altered sulfated proteoglycans from a cloned line of Swiss mouse 3T3 cells. This cell type was selected because it exhibits contact inhibition of ...
The fist goal was to identify changes in mRNA expression and in plasma glycoproteins that are induced by prion infection in mice. The unusual nature of prion disease prompted a systems approach to identify networks specifically perturbed by prion infectio...
ERGIC-53, a homo-oligomeric recycling protein associated with the ER�Golgi intermediate compartment (ERGIC), has properties of a mannose-selective lectin in vitro, suggesting that it may function as a transport receptor for glycoproteins in the early secretory pathway. To investigate if ERGIC-53 is involved in glycoprotein secretion, a mutant form of ...
Glycoproteomics recently became a very active field, mostly in mammals. The first part of this paper consists of a mini-review on the strategies used in glycoproteomics, namely methods for enrichment in glycoproteins and mass spectrometry (MS) techniques currently used. In a second part, these strategies are applied to the cell wall glycoproteome of etiolated hypocotyls of ...
Vesicular stomatitis virus and Sindbis virus are RNA-containing viruses which contain one and two major glycoprotein species, respectively, in their lipid-envelopes. These viruses are excellent models for the study of the biosynthesis of integral membrane glycoproteins, both viral and cellular. Both viruses have an extremely wide host cell range in tissue ...
... abnormalities. Endothelial cell (EC) damage has been identified as a major factor associated with acute respiratory failure. ...
... software development methodology can be adapted for multimedia development. ... that aims to identify lumps or other breast abnormalities that might ...
Microfibril-associated glycoprotein, MAGP (gene symbol MFAP2), is a component of connective tissue microfibrils and a candidate for involvement in the etiology of inherited connective tissue diseases. We have cloned a human MAGP cDNA that is highly homologous to the previously characterized bovine and murine genes. Like the bovine and murine loci, the human gene has eight ...
Exposure of the Chinese hamster ovarian AuxB1 cell line in vitro to fractionated X-irradiation generated sublines designated DXR-10, which proved resistant to multiple drugs and overexpressed P-glycoprotein (Pgp), as judged by Western blotting using the C219 monoclonal antibody. Further characterization of these irradiated DXR-10 sublines has provided evidence for: (i) the ...
The membrane glycoprotein component of the cellular proteome represents a promising source for potential disease biomarkers and therapeutic targets. Here we describe the development of a method that facilitates the analysis of membrane glycoproteins and apply it to the differential analysis of breast tumor cells with distinct malignant phenotypes. The ...
Protein extracts from pregnant mouse endometria were compared with those obtained from non-pregnant and pseudopregnant mice to detect early pregnancy-specific galactose-rich glycoproteins. Gradient gel electrophoresis combined with lectin overlay and lectin histochemistry were used to identify Ricinus communis I (RCA-I), R. communis II (RCA-II) and Cytisus ...
Con A acceptor glycoproteins were analyzed by 2D-PAGE and 125I-Con A overlay in three squamous carcinoma cell lines and compared with those in the simian virus (SV40)-transformed keratinocyte cell line SVK-14 and in normal keratinocytes. The majority of the glycoproteins identified by this technique were expressed at similar levels in ...
Membrane glycoproteins play vital roles in many fundamental physiological and pathophysiological processes in the central nervous system and represent important targets for pharmaceuticals and biomarker discovery. However, their isolation and characterization has been greatly limited. Lectin affinity chromatography (LAC) has evolved as a powerful method to enrich ...
Holoprosencephaly (HPE) is the most common malformation of the human forebrain. When a clinician identifies a patient with HPE, a routine chromosome analysis is often the first genetic test sent for laboratory analysis in order to assess for a structural or numerical chromosome anomaly. An abnormality of chromosome number is overall the most frequently ...
A study of specific interactions between lectins and glycoproteins has been carried out using surface plasmon resonance (SPR) in a flow-injection mode. Lectins were covalently immobilised on the surfaces of the microfluidic sensor chip via amine coupling and serum glycoproteins were injected into the flow channels. Specific ...
Our previous studies on an inhibitor of O-glycosylation of glycoproteins, GalNAcalpha-O-bn, in the model of enterocytic HT-29 cells, have shown at the cellular level an alteration of the normal localization of apical glycoproteins, and at the biochemical level an in situ synthesis and storage of sialylated GalNAcalpha-O-bn oligosaccharides. The purpose of ...
Replacement of polyclonal anti-rabies immunoglobulin (RIG) used in rabies post-exposure prophylaxis (PEP) with a monoclonal antibody will eliminate cost and availability constraints that currently exist using RIG in the developing world. The human monoclonal antibody RAB1 has been shown to neutralize all rabies street isolates tested; however for the laboratory-adapted fixed strain, CVS-11, ...
Identification of glycosylated proteins, especially those in the plasma membrane, has the potential of defining diagnostic biomarkers and therapeutic targets as well as increasing our understanding of changes occurring in the glycoproteome during normal differentiation and disease processes. Although many cellular proteins are glycosylated they are rarely identified by mass ...
Abnormal liver test results indicate underlying liver disease; unfortunately, these tests are neither sensitive nor specific. The pattern of liver test abnormality can identify the cause and can direct further investigations. Abnormal test results should be followed by a thorough history, which often provides more ...
HPV Testing Shows Which Pap Abnormalities Need Attention Testing for the human papillomavirus (HPV) may help doctors and patients decide what to do about the mildly abnormal and very common Pap test result known as ASCUS. Findings from a major, randomized, multicenter study by the National Cancer Institute (NCI) show that HPV testing is highly sensitive in ...
A new megathrombocytopenic syndrome with giant platelets in peripheral blood and severe thrombocytopenia was diagnosed in a 4-month-old boy. His clinical course included repeated hemorrhagic incidents leading to death at age 37 months. Bone marrow ultrastructural analysis revealed numerous dystrophic megakaryocytes with giant membrane complexes. Although these features were similar to those ...
Golgi-localized nucleotide sugar transporters (NSTs) are considered essential for the biosynthesis of wall polysaccharides and glycoproteins based on their characteristic transport of a large number of nucleotide sugars to the Golgi lumen. The lack of NST mutants in plants has prevented evaluation of this hypothesis in plants. A previously undescribed Golgi NST mutant, brittle ...
Dystroglycan is a central component of the dystrophin-glycoprotein complex that links the extracellular matrix with cytoskeleton. Recently, mutations of the genes encoding putative glycosyltransferases were identified in several forms of congenital muscular dystrophies accompanied by brain anomalies and eye abnormalities, and aberrant ...
We proposed to identify, by using a novel phage display technique, carbohydrate-binding proteins (lectins), which may play an important role in progression and metastasis of breast cancer. Using blood- group H- expressing glycoprotein fraction as bait, we...
We proposed to identify, by using a novel phage display technique, carbohydrate-binding proteins (lectins), which may play an important role in progression%on and metastasis of breast cancer. Using blood-group H-expressing glycoprotein fraction as a bait,...
We have reported that chimeric virus 319.1 virus containing the E2 glycoprotein gene from Classical Swine Fever Virus (CSFV) vaccine strain CS with the genetic background of virulent CSFV strain Brescia (BIC virus) was attenuated in pigs. To identify the amino acids mediating 319.1 virus attenuation...
Technology Transfer Automated Retrieval System (TEKTRAN)
The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, ...
