The elastic property of red blood cell is supported by interaction between red cell membrane and the intricate cytoskeleton network underlying the membrane bilayer cytoplasmic face. One of the major scaffold protein linkers is band 3-ankyrin complex. Defects occurring in this complex have been found in many inherited diseases, causing red blood cell ...
PubMed
Several workers have identified molecular abnormalities associated with inherited blood disorders. The present work examines how these alterations in molecular structure affect the viscoelastic properties of the red blood cell membrane. Changes in the membrane shear modulus, the membrane viscosity, and the apparent membrane bending stiffness were observed in cells of eight ...
PubMed Central
The abnormal abundance of (beta) -amyloid plaques and neurofibrillary tangles are the hallmark of Alzheimer's disease (AD). Human central nervous system (CNS) grey matter was probed for characteristics arising from these pathological features. In AD but not normal grey matter, an IR band at 1615 cm-1 is seen, characteristic of a ...
NASA Astrophysics Data System (ADS)
Chromosome translocations involving band 11q23 are associated with human acute leukemias. These translocations fuse the ALL-1 gene, homolog of Drosophila trithorax and located at chromosome band 11q23, to genes from a variety of chromosomes. We cloned and sequenced cDNAs derived from transcripts of the AF-4 and AF-9 genes involved in the most common ...
L cells became giant without cell division after x irradiation with 5000 R and some of them reached 200 times volume of an unirradiated cell. Protein of giant cells was analyzed electrophoretically after treatment with SDS and urea. Electrophoresis of whole polypeptides of giant cells labeled with 3H-leucine and unirradiated cells labeled with /sup 14/C-leucine was ...
Energy Citations Database
1. The fusion of human erythrocytes into multicellular bodies that is induced by microdroplets of oleoylglycerol was investigated by optical and electron microscopy, and by gel electrophoresis of membrane proteins. 2. At the highest concentrations of oleoylglycerol and Ca2+ used, at least 80% of the cells fused after 30min at 37�C and only about 5% of ...
This is the first report of cases of scrapie in Poland. The disease was an atypical phenotype, diagnosed in two aged sheep which were found dead. Brainstem samples from both animals were positive on the applied ELISA rapid test, while the confirmatory immunoblot indicated abnormal banding patterns of protease resistant prion protein ...
... narrow band-pass multilayer dielectric spike filters have been investigated as secondary standards in wavelength calibration of spectrophotometers ...
DTIC Science & Technology
... induces accumulations of an abnormal form of prion protein (PrPres) in nervous and lymphoid tissues. This report ... ...
NBII National Biological Information Infrastructure
... precursor; band 3, Unnamed proteins; band 4, Vitamin D-binding protein precursor; band 5 Apolipoprotein H; band 6, Ceruloplasmin; band 7 ...
Band 3 is the major anion transport polypeptide of erythrocytes. It appears to be the binding site of several glycolytic enzymes. Structurally, band 3 is the major protein spanning the erythrocyte membrane and connects the plasma membrane to band 2.1, which binds to the cytoskeleton. In the present study, we report ...
Band 3 is the major anion transport polypeptide of erythrocytes. It appears to be the binding site of several glycolytic enzymes. Structurally, band 3 is the major protein spanning the erythrocyte membrane and connects the plasma membrane to band 2.1, which binds to the cytoskeleton. In the present study, the ...
Proteomic and immunochemical analyses have shown that hyperphosphorylated TDP-43 is a major component of ubiquitin-positive inclusions from brain of frontotemporal lobar degeneration (FTLD) patients. In 2008, TDP-43 gene mutations were discovered in familial and sporadic amyotrophic lateral sclerosis (ALS), indicating that TDP-43 protein abnormality is ...
Studies of a number of properties of the pathological gammaA-proteins in the first four cases of the recently recognized alpha-chain disease demonstrate that, as in gamma-heavy-chain disease, the abnormal protein is devoid of light chains and represents a portion of the alpha-heavy chain related to the Fc-fragment. In two patients, ...
To evaluate the role of erythrocyte (RBC) membrane proteins in the invasion and maturation of Plasmodium falciparum, the authors have studied, in culture, abnormal RBCs containing quantitative or qualitative membrane protein defects. These defects included hereditary spherocytosis (HS) due to decreases in the content of spectrin ...
The human pim-1 gene was recently identified as a new putative oncogene located on chromosome 6p21, a region showing karyotypic abnormalities in particular leukemias. In the present work the authors characterized the pim protein product. In vitro translation of positively selected poly(A)/sup +/ mRNA indicates that this gene encodes a 33-kilodalton ...
basis of the accu- mulation of abnormal proteins in Progeria and aging fibro- blasts. Basic Life Sci. 35
E-print Network
Chronic myelogenous and acute leukemia are reviewed with regard to data obtained prior to banding of chromosomes, data obtained with banding of chromosomes, and pH-negative patients. Abnormal chromosome patterns of patients are described. Relationships of chromosome abnormalities in acute and chronic myelogenous ...
Excessive amounts of heavy metals adversely affect plant growth and development. Also, the presence of elevated levels of heavy metal ions triggers a wide range of cellular responses including changes in gene expression and synthesis of metal-detoxifying peptides. The overall objective of this research was to elucidate some microscopic effects of heavy metals on the formation, development, and ...
Band heterotopias are one of the rarest groups of congenital disorder that result in variable degree of structural abnormality of brain parenchyma. Band of heterotopic neurons result from a congenital or acquired deficiency of the neuronal migration. MRI is the examination of choice for demonstrating these ...
Evidence is presented that E. coli contains a mechanism for selective degradation of abnormal proteins. Unfinished polypeptides containing puromycin, proteins containing frequent errors in translation, such as those synthesized by strains containing a ram...
National Technical Information Service (NTIS)
The Optic Neuritis Treatment Trial (ONTT) is a prospective study of corticosteroid treatment of acute optic neuritis (ON), with subsequent longitudinal follow-up to determine development of clinically definite multiple sclerosis (CDMS). We analyzed the CSF of 83 patients with clinically isolated ON who underwent lumbar puncture within 24 hours of enrollment into the ONTT to determine the value of ...
The author recalls the main stages of biosynthesis of proteins based on molecular pathology : transcription of DNA into messenger RNA, the genetic code, translation of protein molecules and regulation of their synthesis. Examples are then given of qualitative and quantitative disturbances. Qualitative disturbances with synthesis of ...
We isolated and identified the major protein present in corneas with granular dystrophy (GCD). We compared Coomassie-blue-stained protein bands obtained on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) from the extracts of corneas with GCD, corneas with other disorders, and normal human corneal tissue. After ...
Blue native electrophoresis (BNE) was applied to analyze the von Willebrand factor (vWF) multimers in their native state and to present a methodology to perform blue native electrophoresis on human plasma proteins, which has not been done before. The major difference between this method and the commonly used SDS-agarose gel electrophoresis is the lack of satellite ...
mid1 is required for the proper placement of the contractile actin ring for cytokinesis at a medial site overlying the nucleus. Here we find that mid1 protein (mid1p) shuttles between the nucleus and a cortical medial broad band during interphase and early mitosis. The position of this broad band, which overlies the nucleus, is ...
All studies concerned with karyotype analysis, banding patterns and other microscope techniques for investigating chromosome number, identification and abnormalities are included. An overview of agents resulting in chromosomal or chromatid damage is essen...
A cohort of 16 municipal workers engaged in cleaning oil from old transformers was examined for possible health effects from exposure to polychlorinated biphenyls (PCBs). In addition to the evaluation of routine clinical parameters (history, physical examination, liver function tests, serum triglycerides, serum PCB values), a new screening technique for the presence of oncogene ...
Platycampus larvae are highly cryptic leaf feeders characterised by a dorso-ventrally flattened body, the dorsal integument resembling a shield. Dorsal and ventral cuticles from Platycampus luridiventris were compared by histology and gel electrophoresis. By Azan-staining, a red and a blue layer were distinguished in the dorsal cuticle, while the ventral cuticle showed one, almost uniform blue ...
Chronic myelogenous and acute leukemia are reviewed with regard to data obtained prior to banding of chromosomes, data obtained with banding of chromosomes, and pH-negative patients. Abnormal chromosome patterns of patients are described. Relationships of...
QuickTime movie of SEM series of Drosophila gastrulation, Lateral View, fushi tarazu (ftz) Mutant Showing: Pole Cell Migration; Germ Band Extension; Abnormal Segmentation; Germ Band Shortening
NSDL National Science Digital Library
Protein 4.2 (P4.2), one of the major components of the red-blood-cell membrane, is located on the interior surface, where it binds with high affinity to the cytoplasmic domain of band 3. Individuals whose red blood cells are deficient in P4.2 have osmotically fragile, abnormally shaped cells and moderate hemolytic anemia. cDNA clones ...
Congenital disorders of glycosylation (CDG) form a group of metabolic disorders caused by deficient glycosylation of proteins and/or lipids. Isoelectric focusing (IEF) of serum transferrin is the most common screening method to detect abnormalities of protein N-glycosylation. On the basis of the IEF profile, patients can be grouped ...
Human erythrocytes possess a lattice work of extrinsic proteins on the inner face of the membrane (;cytoskeleton') that maintains the shape and deformability of the cell. The major proteins of the cytoskeleton are spectrin and actin, which are attached to the membrane by protein bands 2.1 (;ankyrin') and 4.1. The ...
The use of blue native polyacrylamide gel electrophoresis (BN-PAGE) has been reported in the literature to retain both water-soluble and membrane protein complexes in their native hetero-oligomeric state and to determine the molecular weight of membrane proteins. However, membrane proteins show abnormal mobility ...
Southeast Asian ovalocytosis (SAO) is a hereditary condition that is widespread in parts of Southeast Asia. The ovalocytic erythrocytes are rigid and resistant to invasion by various malarial parasites. We have previously found that the underlying defect in SAO involves band 3 protein, the major transmembrane protein, which has ...
... the fluorophore in the UV, as confirmed by photoluminescence excitation experiments. No emission band was observed around ... ...
... by SDS polyacrylamide gel electrophoresis (SDS-PAGE). ... for sample preparation, electrophoresis, and staining ... protein banding patterns could be ...
... it possible to detect the abnormal protein in blood. A peptide from the carboxyl terminal region, amino ... labeled peptide in the capillary was 50 amol. Blood was obtained from normal sheep and elk, from...
The transmissible spongiform encephalopathies of domestic livestock are fatal neurodegenerative disorders characterized by accumulation of an abnormal isoform of the host prion protein in brain. Scrapie is the prion disease of sheep and the abnormal protein usually accumulates in lymphoid tissues m...
Technology Transfer Automated Retrieval System (TEKTRAN)
... abstract (free full-text available): Analysis of abnormal prion protein glycoform patterns from chronic wasting disease (CWD)- ... ...
... the Expression of Cyclins and Retinoblastoma Protein in the Development of Breast ... Rb and p16 in these tumors suggests that abnormalities in cell ...
... Descriptors : *CELL STRUCTURE, *BREAST CANCER, MEMBRANES( BIOLOGY), CELLS, PROTEINS, NEOPLASMS, CHAINS, MOLECULAR ...
. In the three athletes diagnosed with ALS, McKee found the abnormal protein TDP-43 in the brain and spinal cord
... and abnormal lipid metabolism is discussed. (Author). Descriptors : *CHOLESTEROL, *FATTY ACIDS, *LIPIDS, *PROTEINS, BIOCHEMISTRY ...
... encephalopathies (TSEs) are thought to be caused by the accumulation of abnormal protease-resistant proteins called prions, which are found in ...
... involve oxidative injury mediated by mitochondrial respiratory abnormalities and through ... protein misfolding will enable the development of effective ...
... and spinal cord transmissible spongiform encephalopathies (such as Creutzfeldt-Jakob disease) in which abnormal proteins cause inflammation in the ...
MedlinePLUS
... We have been studying a family of proteins ... NEOPLASMS, CHROMOSOMES, IMMUNOASSAY, ABNORMALITIES, SKELETON, HIGH SENSITIVITY ...
Amyloidosis occurs when abnormal proteins build up and form deposits. The deposits can collect in organs such as the kidney and heart. This can cause the organs ...
