of decline and nadir of peripheral blood absolute lymphocyte depletion. The "normal" range for absolute lymphocyte count can vary, even in a healthy adult population. See graph...

... decline in absolute lymphocyte count over several hours or days after exposure, and appearance of chromosome aberrations (including dicentrics ...

The absolute CD19+ lymphocytes count is essential for chronic lymphocytic leukemia (CLL) management. At the present time, no standardized flow cytometry (FCM) protocol to measure B-lymphocytes counts is established. The aims of the present study were first to evaluate the ...

In the normal peripheral circulation there exists a subpopulation of lymphocytes that is ultrastructurally distinct. This lymphocyte is identified with the electron microscope by the presence of cytoplasmic microtubulelike inclusions called parallel tubular arrays (PTAs) and contains Fc-receptors for cytophilic antibody. In this study, ...

Despite the use of modern immunochemotherapy regimens, almost 50% of patients with diffuse large-B-cell lymphoma will relapse. Current prognostic models, including the International Prognostic Index, incorporate patient and tumor characteristics. In contrast, recent observations show that variables related to host adaptive immunity and the tumor microenvironment are significant prognostic ...

We have found a significant relationship between blood lymphocyte count and prognosis in 45 patients receiving either total lymphoid irradiation or sham irradiation for chronic progressive multiple sclerosis. Patients with sustained lymphocyte counts less than 900 mm-3 for prolonged periods after treatment showed ...

95% Confidence Limit: If a population of people had the same absolute lymphocyte values counts you entered into the tool, you would have 95% confidence that mean value for...

Experiments were carried out on peripheral blood leukocytes and spleen lymphocytes from 29 male rats that were flown during the Spacelab Life Sciences 1 (SLS-1) nine-day mission on the shuttle Columbia in June 1991 and on appropriate ground controls. On the day of landing, there was a significant decrease in the total white blood cell counts (P < ...

Myasthenic patients showed low absolute T cell counts and low spontaneous and phytohaemagglutinin-induced lymphocyte transformation responses. The abnormalities were particularly associated with late onset disease and female sex, but not with HLA phenotype or autoantibody production. Serum factors inhibitory to ...

The management of infants and children born to human immunodeficiency virus (HIV-l)-infected mothers is complicated by both the presence of maternal antibody during the first 18 months of life and the normal, physiologic decline of CD4+ cell counts and pe...

Photopheresis has been used in the management of rejection of heart and/or lung transplants. Although its mechanism of action remains unknown, irradiated T-helper cell-induced immunosuppression is the main theory. Since transplant recipients are often lymphopenic and lymphocytes are the target cells in phototherapy, we performed this study to determine which factors affect the ...

BackgroundPrevious studies have reported that the adoption of a single-platform flow cytometry cell counting method resulted in lower interlaboratory variation in absolute T cell counts as compared to predicate dual-platform flow cytometry methods which incorporate independent automated lymphocyte ...

BackgroundSarcoidosis is a poorly understood chronic inflammatory condition. Infiltration of affected organs by lymphocytes is characteristic of sarcoidosis, however previous reports suggest that circulating lymphocyte counts are low in some patients with the disease. The goal of this study was to evaluate ...

The relationship between tumour load and immunity in gastrointestinal cancer has been studied by sequential comparison in patients whose tumour has been removed and those whose tumour was found to be inoperable. Total lymphocyte count, absolute and percentage T- and B-lymphocyte counts, effect ...

The relationship between tumour load and immunity in gastrointestinal cancer has been studied by sequential comparison in patients whose tumour has been removed and those whose tumour was found to be inoperable. Total lymphocyte count, absolute and percentage T- and B-lymphocyte counts, effect ...

BackgroundOptimal lymphocyte parameters and thresholds for the diagnosis of chronic lymphocytic leukemia have been proposed by The National Cancer Institute sponsored Working Group and recently updated by the International Workshop on chronic lymphocytic leukemia. However, it is not clear how these criteria apply to patient management ...

A CD4 count External Quality Assessment (EQA) program is important for the clinical monitoring of persons infected with HIV/AIDS. The purpose of the present study was to evaluate the CD4 EQA performance program of the flow cytometer laboratories that perform routine CD4 counts for these patients in Thailand. Stabilized whole blood samples were sent to ...

The blood beryllium lymphocyte proliferation test (BeLPT) is a modification of the standard lymphocyte proliferation test that is used to identify persons who may have chronic beryllium disease. A major problem in the interpretation of BeLPT test results is outlying data values among the replicate well counts ( � 7%). A log-linear ...

A study was made of the bone marrow status in unirradiated zones of 33 patients with stage I-II Hodgkin's disease in complete 9-12 year remission after therapeutic irradiation of the lymphatic collectors of the upper part of the trunk in combination with irradiation of the spleen (16 patients) or splenectomy (17 patients). The total count of myelokaryocytes, myelogram, a ...

Use of the standard dual-platform flow cytometric method for determination of CD4(+) T-lymphocyte counts, which needs both a flow cytometer (FCM) and hematological analyzer, would inevitably lead to increased variability. The development of new single-platform (SP) FCMs that provide direct CD4(+) T-lymphocyte ...

... PNEUMOCYSTIS CARINII, *LYMPHOCYTES, RISK, MANAGEMENT, HUMANS, CELLS, PNEUMONIA, INFANTS, HEALTH, NUMBERS, FAMILY ...

The administering of oxytetracycline, vitamin B/sub 12/, and folic acid is efficient in the recovery from postirradiation leucopenia in whole-body irradiated chicks. Oxytetracycline and vitamin B/sub 12/ combined influenced especially the number of heterophils, and the addition of folic acid resulted in a significant rise of the absolute ...

fibrosis (of pancreas) Cystic Fibrosis 567 Juvenile spondyloarthropathy JS 711 Crohn's disease Crohn's 559 (EMR) data and gene expression measurements in the context of disease to identify genes that may for absolute lymphocyte count across 12 diseases as a proxy for differences in aging. We correlate

A study of peripheral blood mononuclear cell phenotyping and HIV testing was performed in 39 chronic hemodialysis patients. The following abnormalities were found in comparison to a control population: 1) a lower percentage of lymphocytes in the white blood cell differential count, 2) a lower percentage of total T cells, 3) a lower percentage and ...

Immune reconstitution after allogeneic stem cell transplantation protects against opportunistic infections and disease relapse. Identifying the most protective lymphocyte subset would have implications of adoptive immunotherapy. We followed up a case series of 34 allogeneic transplantations for pediatric leukemias, aplastic anemias, or solid tumors. Regardless of baseline ...

Cell mediated immunity (CMI) was assessed by determining total and differential leucocyte and absolute lymphocyte counts, T and B-rosettes, PHA-blast transformation of lymphocytes, lymphocyte migration index (LMI), and DNCB response in 66 sea-level residents, 45 temporary residents, and 24 ...

The leukocyte count showed no remarkable changes. The number of neutral red vacuoles in the neutrophils was increased following x irradiation, while the number of mitochondria in lymphocytes was decreased and the neutral red vacuoles were increased. The refractiveness reached its maximum after 2 hr and returned to normal after 24 hr. Relative ...

BackgroundViral reservoir size refers to cellular human immunodeficiency virus-1 (HIV-1) DNA levels in CD4⁺ T lymphocytes of peripheral blood obtained from patients with plasma HIV-1-RNA levels (viral load, VL) maintained below the detection limit by antiretroviral therapy (ART). We measured HIV-1 DNA levels in CD4^{+ ...}

A biannual external quality assessment (EQA) scheme for flow cytometric lymphocyte immunophenotyping is operational in the Benelux countries since 1996. We studied the effects of the methods used on assay outcome, and whether or not this EQA exercise was effective in reducing between-laboratory variation. Eighty test samples were distributed in 20 biannual send-outs. Per ...

PURPOSE: The short- and long-term effects of (90)Yttrium microspheres therapy for hepatocellular carcinoma (HCC) on peripheral blood lymphocytes are unknown and were therefore examined. METHODS AND MATERIALS: Ninety-two HCC patients were enrolled in a (90)Yttrium therapy study and routine blood counts were examined as part of standard clinical monitoring. ...

Since most patients with Hodgkin's disease benefit from splenectomy, a study was designed to explore whether these beneficial effects could be attributed to the recovery of patients' immunologic reactivity. Using a series of ordinary skin test (PPD-tuberculine, Varidase and Candidin) determination of absolute T and B lymphocyte counts ...

Introduction:? Accurate white blood cell counting (WBC) and differential count by blood analyzers could allow a more informative characterization of granulocyte colony-stimulating factor (G-CSF) mobilized blood (MB), leukapheresis products (LP), and cord blood (CB). However, reliable counting by a blood cell analyzer in this setting is ...

Breast cancer is the most common cancer among women worldwide. Discomfort and fatigue are usually arisen from anticancer therapy such as surgery, radiotherapy, chemotherapy, hormonal therapy, or combination therapy, because of the suppressed immunological functions. Yunzhi (Coriolus versicolor) can modulate various immunological functions in vitro, in vivo, and in human clinical trials. Danshen ...

This work formed part of a series of studies devised to investigate immunological markers which it was considered might reflect individual susceptibility to the development of coalworkers' pneumoconiosis and especially progressive massive fibrosis. Estimations of T and B lymphocytes and white blood cell counts (WBCC) were performed on blood ...

BackgroundIn rural Gambia, birth season predicts infection-related adult mortality, providing evidence that seasonal factors in early life may programme immune development. This study tested whether lymphocyte subpopulations assessed by automated full blood count and flow cytometry in cord blood and at 8, 16 and 52 weeks in rural Gambian infants (N = 138) ...

BACKGROUND:: Enumeration of CD4+ T-lymphocytes is important in the management of HIV. However, standard laboratory systems based on flow cytometry are expensive, complicated and thus unavailable to most resource-limited settings where a low-cost and fully automated point-of-care CD4 testing system is required. In attempts to address this issue, a study was conducted to ...

The flow cytometers that are currently supported by industry provide accurate CD4(+)-T-cell counts for monitoring human immunodeficiency virus disease but remain unaffordable for routine service work under resource-poor conditions. We therefore combined volumetric flow cytometry (measuring absolute lymphocyte ...

Abstract Background aims. Neutropenia following high-dose chemotherapy is associated with a substantial risk of infectious complications. The aim of this study was to identify variables in residual leukocyte subsets during neutropenia that are predictive for neutropenic fever. Methods. Residual leukocytes in the peripheral blood on day 5 after autologous blood stem cell transplantation were ...

Traditionally, the elucidation of genes involved in maturation and aging has been studied in a temporal fashion by examining gene expression at different time points in an organism's life as well as by knocking out, knocking in, and mutating genes thought to be involved. Here, we propose an in silico method to combine clinical electronic medical record (EMR) data and gene expression measurements ...

... COUNTING METHODS, *TRITIUM, *HALF LIFE, HYDROGEN, GASES ... AND NUCLEAR CHEMISTRY ISOTOPES ATOMIC AND MOLECULAR ...

... As in the younger age group, the segmented ... ERYTHROCYTES, LYMPHOCYTES, FEMALES, SEX, LEUKOCYTES, BLOOD COUNTS, BLOOD ...

lymphocyte count,the following condition must be present for this tool to calculate a radiation dose Lymphocyte count is > 0.007 x 109 cells/liter and � 1.35 x 109 cells/liter...

We carried out a cross-sectional study with analysis of the demographic, clinical and laboratory characteristics of patients with metal-on-metal hip resurfacing, ceramic-on-ceramic and metal-on-polyethylene hip replacements. Our aim was to evaluate the relationship between metal-on-metal replacements, the levels of cobalt and chromium ions in whole blood and the absolute ...

In preterm neonates the immune system is thought to be less developed at birth, but very little is known about the actual size of lymphocyte subpopulations, and even less about the maturation of these subpopulations during the first months after a premature birth. To evaluate the development of lymphocyte subpopulations in preterm infants during the first ...

The Spix's macaw (Cyanopsitta spixii) is considered the world's most endangered parrot, with the last wild bird disappearing in 2001 and only 74 birds in captivity. To establish hematologic and plasma biochemical reference ranges and to look for differences relative to sex, age, and season, we obtained blood samples from 46 captive Spix's macaws (23 male, 23 female) housed in aviaries at the Al ...

INTRODUCTION: Recent studies have suggested that the absolute lymphocyte count (ALC) may be a prognostic indicator in malignant diseases, in that those patients who have higher ALC at certain times during treatment may have a better chance of survival. The influence of T cells and natural killer cells in the immune system of the ...

Technological advances in instruments allow the evaluation of many lymphocyte subsets in one step. The aim of this study was to evaluate the new FACSCanto flowcytometer in routine conditions, using a 6 color combination, single platform, whole blood, lysis, no wash protocol. METHODS: Two systems were simultaneously compared on 67 blood samples and external quality controls, ...

Apr 21, 2011 ... Differentail blood count : basophiles. Differentail blood count : eosinophiles. Differentail blood count : lymphocytes ...

CS-0777 is a selective sphingosine 1-phosphate receptor-1 (S1P(1)) modulator under development for treatment of autoimmune conditions. A randomized, double-blind, placebo-controlled study was conducted to assess the safety, tolerability, pharmacokinetics, and pharmacodynamics of single oral doses of CS-0777 in escalating dose cohorts of healthy male participants (0.1, 0.3, 1.0, and 2.5 mg; 6 ...

To identify factors that may predict the development of the acquired immune deficiency syndrome (AIDS) or AIDS related symptoms various immunological measurements were studied in a group of homosexual men attending screening clinics for AIDS in Copenhagen. Fifty seven men whose ratio of T helper lymphocytes to T suppressor lymphocytes (CD4:CD8 ratio) was ...

The concept of whether immune function was related to risk of recurrence was examined in patients with operable breast cancer in whom careful clinical and pathologic staging had been performed. Patients were classified according to the risk of recurrence. The "low risk" group included patients with minimal breast cancer, noninfiltrating cancer, or infiltrating cancer less than 1 cm with negative ...

The aim of the study was the evaluation of significance of immunopathogenic mechanisms in the pathogenesis of minimal change nephrotic syndrome and the study of the ultrastructural changes of peripheral blood formed elements. The observation was carried out on 64 patients with steroid sensitive minimal change nephrotic syndrome. During the manifestation and recurrence of the disease, in the ...

The studies covered 139 men including two control groups: 40 nonsmokers and 41 smokers occupationally exposed to organic solvents. The results of toxicological analysiss of the air revealed the presence of benzene, toluene, xylene and their hydrogenated derivatives, paraffin hydrocarbons, oleins, naphthenes, alcohols (butanol, cyclohexanol), esters (ethylglycol acetate, butyl acetate) and ketones ...

An increased prevalence of autoimmune diseases such as rheumatoid arthritis has been demonstrated in silica-exposed patients. The aim of this study was to determine the peripheral blood lymphocyte phenotype in a population of silicotic workers employed in the slate mines of the district. Silicosis was assessed in 58 patients according to the International Labor Office's ...

Lymphocyte subset reference values used to monitor infectious diseases, including HIV/AIDS, tuberculosis, malaria, or other immunological disorders in healthy children in Cameroon, are lacking. Values for Caucasian cohorts are already being utilized for clinical decisions but could be inappropriate for African populations. We report here the immunological profile for children ...

We have examined the effect of different variables such as tissue culture media, with or without various supplements, lymphocyte isolation techniques, lymphocyte contamination by autologous red blood cells and platelets, and lymphocyte numbers, on the requirement for serum during phytohaemagglutinin (PHA) induced DNA synthesis in human ...

... of mouse peripheral blood tested were total white blood cells and the absolute numbers of polymorphonuclear leucocytes, monocytes, lymphocytes ...

To determine whether immunosuppression by total lymphoid irradiation (TLI) slowed deterioration of chronic progressive multiple sclerosis (MS), functional impairment score and blood lymphocyte counts were compared at 6-month intervals through 4 years following treatment of MS patients by either TLI (n = 27) or sham irradiation (n = 21). At each interval, ...

An attempt was made to evaluate the humoral and cellular immune status of preterm and small for dates babies born at All India Institute of Medical Sciences Hospital. The study sample included 24 term small-for-dates babies and 12 preterm babies (gestation of less than 37 weeks) and 20 term appropriate-for-dates babies who served as controls. The small-for-dates babies were subdivided into the ...

BackgroundThe World Health Organization (WHO)'s "3 � 5 program" has spurred efforts to place 3 million people on combination antiretroviral therapy (ART) for treatment of AIDS in resource-limited countries. Paradoxically, the cost of CD4⁺ T-lymphocyte count essential for decision-making to commence HIV positive adults on ART as ...

The blood beryllium lymphocyte proliferation test (BeLPT) is a modification of the standard lymphocyte proliferation test that is used to identify persons who may have chronic beryllium disease. A major problem in the interpretation of BeLPT test results is outlying data values among the replicate well counts (approximately 7%). A ...

The blood beryllium lymphocyte proliferation test (BeLPT) is a modification of the standard lymphocyte proliferation test that is used to identify persons who may have chronic beryllium disease. A major problem in the interpretation of BeLPT test results is outlying data values among the replicate well counts ({approx}7%). A log-linear ...

Several works have endorsed a significant role of the immune system and inflammation in the pathogenesis of heart failure. As indirect evidence, an association between a low relative lymphocyte count (RLC%) and worse outcomes found in this population has been suggested. Nevertheless, the role of RLC% for risk stratification in a large and nonselected ...

Community respiratory viruses (CRVs) have been recognized as a potential cause of pneumonia and death among hematopoietic stem cell transplantation (HSCT) recipients and patients with hematologic malignancies. We reviewed the Microbiology Laboratory records dated from July 1, 2000, to June 30, 2002, to identify patients who had respiratory specimens positive for influenza, parainfluenza, ...

Cases with abnormal blood pictures due to radiation exposures were picked from the radiological workers at Tohoku University to determine characteristic blood pictures of occupational radiation hazards. The study was carried out on persons with less than 1500 lymphocytes and less than 5000 leukocytes for 2 successive examinations. Decreased total leukocyte and ...

IntroductionHerpes simplex virus type 1 encephalitis presents acutely in patients who are immunocompetent. We report what we believe to be the first published case of a subacute course of herpes simplex virus type 1 encephalitis in a patient with asymptomatic chronic lymphocytic leukemia who subsequently developed multiple sclerosis.Case presentationA 49-year-old Caucasian ...

Cytogenetic and haematological parameters were studied in 31 oil exposed workers and 31 office workers matched for age and smoking, all men employed by a Norwegian cable manufacturing company. Information was obtained about tobacco and alcohol consumption, infections, allergies, chronic diseases, use of medicines, and exposure to radiography. A decrease in the absolute ...

Cytogenetic and haematological parameters were studied in 31 oil exposed workers and 31 office workers matched for age and smoking, all men employed by a Norwegian cable manufacturing company. Information was obtained about tobacco and alcohol consumption, infections, allergies, chronic diseases, use of medicines, and exposure to radiography. A decrease in the absolute ...

TRU-016, under development by Trubion Pharmaceuticals Inc and Facet Biotech Corp, is an intravenously administered anti-CD37 IgG fusion protein for the potential treatment of B-cell malignancies, including chronic lymphocytic leukemia (CLL) and non-Hodgkin's lymphoma (NHL), as well as for autoimmune and inflammatory diseases. TRU-016 was created by humanizing SMIP-016, a ...

Groups of mice were killed 1, 3, 7 and 15 days after whole body exposure to 0, 5, 50 or 500 rads of irradiation. Lymphocytes were harvested from the peripheral blood and spleen for total and differential leukocyte counts, for determination of the absolute numbers of thymic-dependent (T) and bone marrow-dependent (B) cells and the in ...

A method of absolute nuclear material assay of an unknown source comprising counting neutrons from the unknown source and providing an absolute nuclear material assay utilizing a model to optimally compare to the measured count distributions. In one embodiment, the step of providing an absolute ...

... Report Date : 23 AUG 1972. Pagination or Media Count : 28. Abstract : Absolute electron yields from tantalum, molybdenum, copper and aluminum ...

... consistent with chronic T-lymphocytic leukemia. Treatment with chlorambucil and prednisone effectively decreased the lymphocyte count, but was associated with thrombocytopenia, which resolved after chloram...

Participation in external quality assessment is an integral part of laboratory work and mandatory when the results have a clinical application, which is one of the requirements of standard 15189 for accreditation of medical laboratories. Institute of Clinical Chemistry, the first laboratory accredited for clinical cell analysis by flow cytometry in Croatia, participated in UKNEQAS for Leukocyte ...

Serum antigen and antibody values were studied in 164 infants and children infected perinatally with HIV. HIV antigens p17, p24, gp41, and gp120 were determined in sera by immunoblot and antigen capture assays. Lymphocyte blast transformation, serum immunoglobulins, and circulating immune complexes were also evaluated. Altogether 50 patients had HIV antigens measured: 31 (62%) ...

... Significant differences on Lymphocytes and Granulocytes counts were found between male and female Aotus when hematological and renal ...

... Scoring (physically counting) of chromosome aberrations (damaged genetic material) in peripheral blood lymphocytes is presently accepted as the ...

Differential white blood cell count (dWBC) is a frequently used diagnostic tool. For most patient samples an automated blood counter produces a five-part differential count. If this dWBC does not meet pre-set criteria, microscopic dWBC is performed. Microscopy is labor intensive and requires sustained training of technicians. Inter-observer variation and ...

BackgroundConcomitant infections may influence HIV progression by causing chronic activation leading to decline in T-cell function. In the Americas, visceral (AVL) and tegumentary leishmaniasis (ATL) have emerged as important opportunistic infections in HIV-AIDS patients and both of those diseases have been implicated as potentially important co-factors in disease progression. We investigated ...

IKO monoclonal antibodies were used to study peripheral blood mononuclear types in 92 patients with various histomorphological types of diffuse lymphosarcoma at various stages of tumorous process. Absolute counts of CD7 and CD5 cells (T cells) were found reducing as was CD4 cell (T helpers) level as the disease progressed. CD8 cell (T suppressors) ...

Ten individuals received an average of 0.211 roentgens of gamma radiation per week for a 77 period week (December 1946-June 1948) for a total average dose of 16.21 roentgens. During this period these 10 men carried out an experiment involving materials which emit gamma radiation and were monitored by daily film badges. The radiation delivered during a week was received in a five day work week and ...

High-dose (HD) IL-2 is approved to treat renal cell carcinoma (RCC) with modest response rates and significant toxicity. Enhancement of cytotoxic T-cell activity by IL-2 is 1 mechanism of action. IL-2 also stimulates regulatory T lymphocytes (Tregs), which are associated with poor prognosis. Favorable outcomes are associated with greater rebound absolute ...

Radiation accidents may produce external or internal contamination with radionuclides, local or whole-body exposure to penetrating radiation, or a combination of these. Trauma and medical problems which require rapid diagnosis and treatment may accompany a radiation injury. The role of the medical technologist in the early post-injury period may include the use of precautions in handling of ...

The treatment of radiation injuries in a disaster situation is discussed. It is pointed out that no new symptoms or signs are present that have not been observed in ordinary clinical practice. This is not a mystery disease. Little can be done for patients in the immediate period after exposure, except for reassurance, sedation, and, possibly, minimal fluid replacement. Patients who ...

BackgroundAlthough renal involvement in advanced haematological malignancies is common, glomerulonephritis associated with lymphoproliferative disorders is rare, and the related pathogenetic mechanisms are still poorly understood. We present a rare case of chronic lymphocytic leukaemia(CLL)-associated focal segmental glomerulosclerosis with nephrotic-range proteinuria.Case ...

Oral fingolimod signals the sphingosine 1-phosphate receptor and this in turn mediates immunomodulatory activity. No data of fingolimod in any pediatric population existed before this study. We put our study results in perspective against data from adult renal transplant patients. We investigated pharmacokinetics and pharmacodynamics of single-dose fingolimod (0.07 mg/kg) and its effects on ...

Generation of B and plasma cells involves several organs with a necessary cell trafficking between them. A detailed phenotypic characterization of four circulating B-cell subsets (immature-, na�ve-, memory- B-lymphocytes and plasma cells) of 106 healthy adults was realized by multiparametric flow cytometry. We show that CD10, CD27 and CD38 is the minimal combination of ...

Thirty-four gnotobiotic dogs were used to study the effects of R252 canine distemper virus (CDV) infection on the lymphoid system. Twenty-one dogs were inoculated parenterally, and 6 dogs were infected by contact exposure to inoculated littermates. Seven littermate dogs were maintained as uninfected controls. The means of the absolute lymphocyte ...

... disease severity. In Study 012, the success rates based on EGSS and mean absolute change in lesion count were marginally higher in the ...

... Report Date : SEP 1965. Pagination or Media Count : 31. Descriptors : *MANOMETERS, HEAT TRANSFER, TRANSPIRATION, FLOW FIELDS. ...

... Pagination or Media Count : 58. Abstract : Diabetes mellitus is a chronic and incurable disorder involving a relative or absolute lack of insulin. ...

... source in water and in two aluminum and water mixtures. ... By use of experimentally determined counting efficiencies, absolute saturation activities ...

... the hand. The puncture site is cleaned with antiseptic, and an elastic band is placed around the ... or young child, the area is cleansed with antiseptic and punctured with a sharp needle or a ...

Absolute and relative detector efficiencies measured. Apparatus uses coincidence-counting techniques

... Pagination or Media Count : 20. Abstract : The shrinkage of resin and thermal expansion during the curing of epoxy (epon 826) were measured. ...

To further define intraepithelial lymphocytes (IELs) in celiac disease (CD) and giardiasis, IELs were probed for the presence of cytolytic granules containing granzyme B (GrB) and T-cell-restricted intracellular antigen (TIA)-1. The expression of TIA-1, GrB, CD3 (T-cell-receptor-associated complex), and Leu-7 (subset of natural killer cells) was studied by a sensitive ...

Lymphocytes from the thoracic duct of rats were injected intraperitoneally into homologous rats previously exposed to 900 rads of total- body radiation. Repeated white blocd cell counts, platelet counts, polychromatophilia counts, and reticulocyte counts provided no evidence that the ...

Alternative donor stem cell transplantation from cord blood or haploidentical peripheral blood donors is increasingly being used for patients who lack a matched related or unrelated donor. A higher nonrelapse mortality (NRM) rate has been noted with these 2 types of transplants, primarily because of infectious complications. Here, we hypothesized that the time to lymphocyte ...

We used monoclonal antibodies and an indirect immunoperoxidase technique to identify mononuclear inflammatory cells associated with human tumors. The absolute number of the different types of inflammatory cells was assessed by using a point-counting technique. We studied tissues from six primary cutaneous melanomas, six metastatic melanomas, eight ...

Lymphocyte chemoattractant factor (LCF) is a lymphocyte cell product that stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. In concert with its chemoattractant activity, LCF induces human T-lymphocyte expression of interleukin 2 receptor. Here we describe the molecular cloning of cDNA ...

Eleven patients with intractable rheumatoid arthritis were treated with total lymphoid irradiation (total dose, 2000 rad) in an uncontrolled feasibility study, as an alternative to long-term therapy with cytotoxic drugs such as cyclophosphamide and azathioprine. During a follow-up period of five to 18 months after total lymphoid irradiation, there was a profound and sustained suppression of the ...

Eleven patients with intractable rheumatoid arthritis were treated with total lymphoid irradiation (total dose, 2000 rad) in an uncontrolled feasibility study, as an alternative to long-term therapy with cytotoxic drugs such as cyclophosphamide and azathioprine. During a follow-up period of five to 18 months after total lymphoid irradiation, there was a profound and sustained suppression of the ...

The absolute numbers of circulating white cells and lymphocyte subpopulations were studied in 25 final-year dental students and compared with a control group of 28 medical students. The total lymphocyte count, total T cell numbers (CD3), T helper/inducer (CD4), and T suppressor/cytotoxic (CD8) numbers were ...

Although fast green (FG) is widely used as food colorant by the cosmetics and drug industries, there is no evidence about whether FG affects leukocytes. The aim was to investigate the effect of FG on leukocytes. Male albino mice were subdivided into five equal groups: four of them were given 125, 250, 375 and 500 mg kg(-1) bw day(-1) FG in drinking water for 44 days. The fifth (control group) was ...

In the evaluation of the active alveolitis of pulmonary sarcoidosis, both the proportional lymphocyte count obtained by bronchoalveolar lavage and state of activation of the alveolar macrophage by gallium scanning are required. We injected 6 mCi (200 MBq) of /sup 67/Ga intravenously on 24 occasions in 13 patients with biopsy-proved sarcoidosis. Forty-eight ...

BACKGROUND: Platelet counts below normal values define thrombocytopenia. However, platelet counts alone do not reveal the underlying pathomechanism. New blood cell counters provide additional information on platelet size and volume, and enable the distinction of sub-populations. In this preliminary study, we evaluate whether one of these markers can be ...

BACKGROUND: Platelet counts below normal values define thrombocytopenia. However, platelet counts alone do not reveal the underlying pathomechanism. New blood cell counters provide additional information on platelet size and volume, and enable the distinction of sub-populations. In this preliminary study, we evaluate whether one of these markers can be ...

... early diagnosis of HIV-1 infection in infants and children ... guide decisions regarding the institution of Pneumocystis carinii pneumonia prophy- laxis ...

Immunophenotyping studies of peripheral blood lymphocytes indicated a significant decrease in the absolute number of B-cells, T-helper cells, ...

Background & objectives: Perforin is one of the major effector molecules of cytotoxic cells associated with killing of cells harbouring intracellular bacterial infection. The precise role of perforin positive cells in tuberculosis still remains controversial. The present study was done to determine the number of circulating CD4 + and CD8 + perforin positive cells to assess the level of ...

According to the classification of chronic lymphocytic leukemia (CLL) proposed by A. I. Vorob'ev and M. D. Brilliant in 1983, benign CLL is a distinct form of CLL which is characterized by low level of absolute lymphocytosis, absent or mild peripheral lymphadenopathy, slow progression. No specific therapy is needed. The paper presents clinical, ...

Several small-scale studies have reported pulmonary toxicity among patients with diffuse large B-cell lymphoma (DLBCL) receiving rituximab-containing chemotherapy, though whether the use of rituximab predisposes to interstitial pneumonia (IP) remains unclear. This retrospective study was intended to identify the characteristics and risk factors of IP in patients with DLBCL. Between 2000 and 2009, ...

To assess the effects of radiotherapy on immune status, a wide range of assessments have been performed on patients with localized carcinoma of the cervix uteri undergoing and initially responding clinically to radical pelvic radiotherapy. neutrophil function was essentially unchanged by treatment. Absolute neutrophil and lymphocyte ...

IntroductionAlthough major changes of the immune system have been described in sepsis, it has never been studied whether these may differ in relation to the type of underlying infection or not. This was studied for the first time.MethodsThe statuses of the innate and adaptive immune systems were prospectively compared in 505 patients. Whole blood was sampled within less than 24 hours of advent of ...

In 2000, the Belgian Scientific Institute of Public Health introduced a voluntary external quality assessment scheme for lymphocyte immunophenotyping. This paper provides an analysis of the first six surveys. Specimens consisted of fresh EDTA-anticoagulated whole blood and were sent by overnight mail. The 41 participants were surveyed for methodology and were asked to report ...

Cell survival kinetics in both peripheral blood and in bone marrow have been studied over the time course of hyperfractionated total body irradiation (TBI) for bone marrow transplantation. Our unique TBI regimen allows the study of the in vivo radiation effect uncomplicated by prior cyclophosphamide, since this agent is given after TBI in our cytoreduction scheme. Peripheral blood cell ...

A high count rate 4 pi beta - gamma coincidence counting system for the determination of absolute disintegration rates of short half-life radionuclides is described. With this system the dead time per pulse is minimized by not stretching any pulses beyond...

Tumor lysis syndrome (TLS) has been described in over 40% of patients with chronic lymphocytic leukemia treated with the cyclin-dependent kinase inhibitor, flavopiridol. We conducted a retrospective analysis to determine predictive factors for TLS. In 116 patients, the incidence of TLS was 46% (95% CI: 36-55%). In univariable analysis, female gender, greater number of prior ...

Blood samples were collected from the bill sinus of nine free-living platypuses (Ornithorhynehus anatinus) within 12 min of capture of each and again after 1 to 12 hr, New South Wales, Australia, 1981 to 1988. In seven animals which were not anesthetized, there was a significant (P < 0.01) fall in lymphocyte count between the two samples. The reduction ...

Changes in peripheral blood cell levels were followed in rats exposed to x-ray doses of 600 r, some of which were pre-treated with 10 mg of this compound, previously shown to be radioprotective in mice. This dose proved absolutely lethal for the unprotected animals, which died between the 10th and 24th day, but 80% of the treated animals survived to at least the 30th ...

Background A combination of horse anti-thymocyte globulin and cyclosporine produces responses in 60-70% of patients with severe aplastic anemia. We performed a phase II study of rabbit anti-thymocyte globulin and cyclosporine as first-line therapy for severe aplastic anemia. DESIGN AND METHODS: Twenty patients with severe aplastic anemia treated with rabbit anti-thymocyte globulin were compared to ...

Four arc welders having a flu-like illness with multiple health complaints following an exposure to high concentrations of isocyanate fumes from ignited polyurethane foam underwent immunological tests as follows: ELISA antibody assays, activated lymphocyte profiles, and lymphocyte blastogenesis. ELISA procedures revealed the presence of antibodies to ...

Peripheral blood lymphocytes (PBL) were obtained from HIV-1-infected patients at different stages of disease. The absolute number of IgM-, IgG-, and IgA-producing lymphocytes per 10(6) PBL was increased 2.8-, 3.4-, and 1.9-fold, respectively, compared with normal controls. 2-17% of IgG-secreting patient cells reacted with the gp160 ...

BackgroundThe pandemic by the novel H1N1 virus has created the need to study any probable effects of that infection in the immune system of the host.Methodology/Principal FindingsBlood was sampled within the first two days of the presentation of signs of infection from 10 healthy volunteers; from 18 cases of flu-like syndrome; and from 31 cases of infection by H1N1 confirmed by reverse RT-PCR. ...

A mixed breed dog was diagnosed with large granular lymphocytic leukemia. Immunophenotypic analysis indicated the lymphocytes were CD3+, CD8+ T cells expressing the alpha beta T cell receptor and a leukointegrin, alpha d. Chemotherapy and splenectomy resulted in an initial reduction in the lymphocyte ...

Serial leucocyte counts were done on 15 full-term babies during the first 10 days of life and on 14 prematures during the first 30 days. In addition a single count was done on the total of 53 babies around the 96th hour of life. Absolute values of each cell type are given for each postnatal age examined. The main changes in the ...

Investigated effects of recent life events, psychological adjustment, and social support on lymphocyte count among 192 older adults. For males, recent sexual dysfunction lowered lymphocyte count, whereas psychological adjustment and percentage kin in intimate network elevated it. For females, family or legal ...

The lymphocyte proliferation test (LPT) is a noninvasive screening procedure used to identify persons who may have chronic beryllium disease. A practical problem in the analysis of LPT well counts is the occurrence of outlying data values (approximately 7% of the time). A log-linear regression model is used to describe the expected well ...

... varies with age. Causes References Laboratory reference values. Hematology group (Lymphocyte). Rochester, Minn.: Mayo Foundation for Medical Education and Research; Nov. 2010. Hematology. In: Wallach J. Interpretation of Diagnostic Tests. 8th ...

... some studies are looking into blood and marrow stem cell transplants . This procedure may help treat or cure some of the conditions that can cause a low lymphocyte count. Other studies are looking at medicines and other substances that can help the body make more lymphocytes.

Many previous studies of immune reconstitution (IR) postallogeneic hematopoietic stem cell transplantation (HSCT) have focused on lymphocyte recovery. Recognizing that IR involves complex interactions between innate and adaptive immune networks, we hypothesized that patterns of both monocyte and lymphocyte recovery could provide additional prognostic ...

efficiency of photon-counting detectors without reference to any externally calibrated detector standard [3 variation of the absolute response of a photon counting system can be measured at a range of wavelengthsmetrologia Absolute detector quantum-efficiency measurements using correlated photons A. L. Migdall

To test the supposition that during the day changes occurred in the blood picture of people subject to occupational radiation exposures, workers with marked decreases of leukocyte counts and workers who had recovered from the effects of excessive radiation were selected for study from radiological employees and x-ray techniciane. In the cases of long-standing service ...

A method for absolute calibration of proportional counters for pulsed fast neutrons is presented. The method is based on the use of an isotopic standard source and development of a model for counting detected events from area of a signal compounded by single piled up neutron pulses. Effects of detection counting statistics and ...

A method for absolute calibration of proportional counters for pulsed fast neutrons is presented. The method is based on the use of an isotopic standard source and development of a model for counting detected events from area of a signal compounded by single piled up neutron pulses. Effects of detection counting statistics and ...

This report presents the results of a measurement of the half-life of tritium by absolute counting. The method consisted in the mass-spectrometric analysis of a hydrogen-tritium mixture enriched in tritium, the dilution of some of this material with hydrogen in a known way to a countable level of activity, and the counting of a portion ...

The correlation counting method is extended to two-step cascade processes with relatively long-lived intermediate states. Expressions are given for the statistical uncertainties and deadtime effects. The technique is applied to the absolute standardisation of 75Se, 67Ga, 67Cu and 85Sr activities, and to the detection and estimation of afterpulses in liquid ...

The ability of neutron probes to provide accurate and reproducible soil moisture measurements is discussed. It is stressed that sensitivity of the probe in terms of soil moisture measurements is of greater interest than efficiency in terms of absolute counts or counting rate per millicurie. The importance of calibration curve ...

Differential blood count. Lymphocytes, percent. Neutrophiles, percent. Band cells, percent. Monocytes, percent. Eosineophiles, percent. Basophiles, percent ...

... The principal dynamic effects are transient decreased heart rate, transient AV conduction blocks and a mean lymphocyte count reduced by approximately 70% from ...

... This is uniquely different from the plateau of effect seen with lymphocyte count and heart rate. 2.2.5 Is there any significant exposure-response relationship? ...

The purpose of this study was the characterization of the different subsets of lymphocyte cells in dogs with subclinical ehrlichiosis in order to contribute to the knowledge of the immune response developed in the course of this disease. Thirty-eight dogs with subclinical ehrlichiosis and 15 unaffected dogs from two shelters in the area of Valencia (eastern Spain) were ...

The leucocyte count and blood formula were determined at 3, 9, 16, 21, 30, 90, l80, 270, and 360 days after the irradiation of rats with a dose of 500 r. At 30 days the per cent and absolute number of segmented neutrophils was twice the initial level, and the per cent of lymphocytes was 68.3%, compared to 81.7% before ...

The incidence of B cell leukaemia in 186 consecutive untreated patients with histologically defined B cell neoplasms is described. The lymphomas were classified by the Kiel convention. B cell leukaemia in the context of this paper refers to the situation where a neoplastic clone of B cells in the blood greatly outnumbers normal blood B cells. It is defined as an absolute blood ...

Sep 18, 2007 ... The unmodeled variance in the background rate, which fundamentally ... Faint Sources (Absolute Flux Measurements) - For absolute X-ray flux .... for dead time using the excess count rate above the background level; ...

We report on progress made mapping Aristarchus Crater using high-resolution LROC NAC images. Observations include enigmatic impact melt features and stratified blocks of ejecta. An absolute model age using crater counts was also determined.

BackgroundMalaria is the commonest cause of childhood morbidity in Western Kenya with varied heamatological consequences. The t study sought to elucidate the haemotological changes in children infected with malaria and their impact on improved diagnosis and therapy of childhood malaria.MethodsHaematological parameters in 961 children, including 523 malaria-infected and 438 non-malaria infected, ...

Liquid scintillation counting of trichloracetic acid extracts of [3H] TdR-labelled lymphocytes has been studied by a direct suspension method without previous solubilization. In this new method, mixing of the labelled powder in water suspension with Scintillator Emulsifying Mixture (SEM) results in a firm gel whose mechanical properties provide an ...

Treatment of mice with colchicine or trimethylcolchicine acid methyl ether prior to irradiation caused an increase in bone marrow cellularity which was apparent 3 days after LD/sub 5/ irradiation; no significant increase in granulocyte counts 3 to 4 days after irradiation; a relatively small but significant increase in lymphocyte ...

In vitro studies showed that several cytokines produced by the immune system can play a relevant role in modulating bone turnover, thus affecting the health of bone tissue. Our aim was to evaluate the association between total lymphocyte count (TLC) and femoral bone mineral density (BMD) in a sample of postmenopausal women. We studied 114 out of 124 ...

The potential hazards from exposure to beryllium or beryllium compounds in the workplace were first reported in the 1930s. The tritiated thymidine beryllium lymphocyte proliferation test (BeLPT) is an in vitro blood test that is widely used to screen beryllium exposed workers in the nuclear industry for sensitivity to beryllium. Newman [18] has discussed the clinical ...

A community was exposed for several days to formaldehyde (HCHO), hexamethylenetetramine, trimethylamine, and paraformaldehyde emitted from an overheated tanker car containing ureaformaldehyde resin. Residents experienced acute HCHO symptoms at the time of the accident. Many developed chronic, multiple organ health complaints. Three years following the accident, exposed subjects were compared to ...

A community was exposed for several days to formaldehyde (HCHO), hexamethylenetetramine, trimethylamine, and paraformaldehyde emitted from an overheated tanker car containing ureaformaldehyde resin. Residents experienced acute HCHO symptoms at the time of the accident. Many developed chronic, multiple organ health complaints. Three years following the accident, exposed subjects were compared to ...

The potential hazards from exposure to beryllium or beryllium compounds in the workplace were first reported in the 1930s. The tritiated thymidine beryllium lymphocyte proliferation test (BeLPT) is an in vitro blood test that is widely used to screen beryllium exposed workers in the nuclear industry for sensitivity to beryllium. The clinical significance of the BeLPT was ...

About 2 million children worldwide are infected with HIV.There are about 1500 HIV-infected children in France. This review examines the choice of first-line antiretroviral treatment for children under 12 years of age, based on a review of the literature using the standard Prescrire methodology. In children under 5 years of age, the percentage of CD4+ T lymphocytes among all ...

Albumin is an important plasma protein which is useful in the assessment of in vivo membrane permeability in the lung. In subjects with interstitial lung disease (ILD) the relationship between albumin recovered from bronchoalveolar lavage (BAL) and other markers of inflammatory activity may provide useful information of the pathogenesis of the disease process. Unfortunately, its measurement is ...

PurposeTo define the optimal dose of Polyphenon E for chronic daily administration and tolerability in patients with chronic lymphocytic leukemia (CLL).Patients and MethodsPreviously untreated patients with asymptomatic Rai stage 0 to II CLL were eligible for participation. Polyphenon E with a standardized dose of epigallocatechin-3-gallate (EGCG) was administered using the ...

As a kind of E3 ligase, the product of FBXL4 gene belongs to a member of FBLs which is the biggest eukaryotic subfamily of F-BOX proteins, it can recognize some substrate through particular protein-protein interaction domains. To investigate its functions, the polymorphism and association analysis was analyzed. The partial cDNA of porcine FBXL4 with 2384 bp long was first cloned; the deduced ...

Summary Objective To determine the normal haematological and immunological reference intervals for healthy Tanzanian children. Methods We analysed data from 655 HIV-seronegative, healthy children from 1 month to 18 years of age from the Kilimanjaro Region of Tanzania for this cross-sectional study. Median and 95% reference ranges were determined for haematological and immunological parameters and ...

Rats were sacrificed 48 hrs after x irradiation of the thymic region and differential cell-counts were made on sections to determine the lymphocyte reticulum-cell ratio in cortex and medulla. Assuming the reticulum cells unaffected, the decrease in the ratio compared with controls gave the decrease in lymphocyte population. ...

... data and absolute bird counts. This will entail learning R (programming language) and using methods such as zero-inflated poisson ... ...

... Pagination or Media Count : 14. Abstract : We present a method for estimating the absolute frequency stability of N clocks separate from a reference. ...

Methodology is presented for conducting drive-line counts to obtain absolute numbers of deer within fairly small (

... specimen controls (individuals with known chromosome number). For chromosome counts, floral buds or root tips were fixed in Carnoy's solution (absolute ethanol ... ...

... RESEARCH DEPT. Personal Author(s) : Cope,Freeman W. Report Date : 21 MAY 1968. Pagination or Media Count : 13. Abstract ...

BACKGROUND:: Parainfluenza virus (PIV) infections are an important cause of morbidity in children with upper or lower respiratory tract infection (URTI and LRTI, respectively). However, the epidemiology of PIV infections in children with cancer has not been well studied. METHODS:: This retrospective study sought to determine the epidemiology of PIV infections and risk factors for progression to an ...

A group of 20 male rats of mean initial body weight of 65.53 g were irradiated for 7 weeks (5 d per week, 4 h per d) with an electromagnetic pulse field of the following parameters: working frequency 2736.5 MHz; repeated frequency 395 Hz; pulse width 2.6 musec; vertical polarization; mean power density 24.4 mW/cm2; accuracy of measuring +- 6%. The rectal temperature of experimental animals ...

Apoptosis is now recognized as a central process of development and disease, and it has been proposed as one of the mechanisms that may account for the lymphopenia seen in some diseases. In this study we measured spontaneous apoptosis and CD95 expression on different cell subpopulations from CVID patients, using flow cytometric techniques. We divided our patients into two groups according to their ...

AIM: To assess the peripheral T lymphocyte subsets in chronic hepatitis B virus (HBV) infection, and their dynamics in response to adefovir dipivoxil monotherapy.METHODS: Proportions and absolute counts of peripheral natural killer cells, B cells, CD8+, CD4+, CD8+CD38+, CD8+CD28+ and CD4+CD28+ T cells were determined using three-color ...

The distribution of small lymphocytes according to life-span in the peripheral lymphoid tissues of the mouse mutant "nude" has been studied by means of auto-radiography and scintillation counting to evaluate the localization of B lymphocytes with varying life-span. The vast majority of the lymphocytes in this ...

The effect of x rays on lymphocytes of man and lower animals was studied by the slide-chamber method and the method of unstained cell counts. Large doses of x rays (4000 r or more) killed lymphocytes by delayed fixation. Moderate doses (1000 r or less) killed lymphocytes by intranuclear vacuolization. ...

The paper considers criteria for evaluating postoperative protein-energy deficiency (PED) in surgical patients with extensive purulent soft tissue wounds (PSTW) of the extremities and torso. The authors have evaluated the clinical significance of somatometric indices (height (cm), weight (kg), arm circumference (cm), skinfold thickness above the triangularis, body mass index) and biochemical ...

Thirty-one patients receiving radiation therapy for localized cancer of the head and neck areas were systematically assessed before, during, and after treatment. The pathogenesis of weight loss and its association with treatment morbidity and other determinants were sought. The serial data collected consisted of a food frequency questionnaire based on Canada's Food Guide, anthropometric ...

Thirty-one patients receiving radiation therapy for localized cancer of the head and neck areas were systematically assessed before, during, and after treatment. The pathogenesis of weight loss and its association with treatment morbidity and other determinants were sought. The serial data collected consisted of a food frequency questionnaire based on Canada's Food Guide, anthropometric ...

The dietary supplement Citicoline free-base (choline cytidine 5'-pyrophosphate) was toxicologically evaluated in Sprague-Dawley rats using oral gavage. In an acute 14-day study, 2000 mg/kg was well tolerated. In a 90-day study, 100, 350, and 1000 mg/kg/day doses resulted in no mortality. In males, slight significant increases in serum creatinine (350 and 1000 mg/kg/day), and decreases in urine ...

This experiment was conducted to gain information about the amount of protection from direct blast effects that may be provided by foxholes of uniform dimensions located within distances of a nuclear explosion that are recognized as lethal for combinations of thermal and ionzing radiations and indirect blast injuries. Sixteen dogs protected in foxholes were exposed in pairs to the nuclear ...

The aim of this study was to investigate the effects of human tryptases on inflammatory cell accumulation in vivo. Various concentrations of purified lung or skin tryptases were injected into the peritoneum of BALB/c mouse. At 6 hours or 16 hours following injection, cells from the peritoneal lavage were collected and stained with modified Wright's stain. Differential cell ...

To determine the role of polymorphonuclear (PMN) leukocyte transfusions in neonates with sepsis, 23 consecutive newborns were prospectively randomly selected during an 18-month period in a treatment plan to receive polymorphonuclear leukocyte transfusions with supportive care or supportive care alone. Thirteen neonates received transfusions every 12 hours for a total of five transfusions. Each ...

The CD4+ T lymphocytes are the crucial cells in the cascade of events in forming immune response to the foreign antigen and hence monitoring the CD4+ T cell counts to understand the extent of immune deficiency is a common practice. CD4+ T cells are also the primary target cells for human immunodeficiency virus (HIV). Hence CD4+ T ...

The total number of lymphocytes in the thymus markedly decreased during the first 5 days after x irradintion. Lymphocyte depletion in the first phase was greatest in the thymus. This suggests that the thymic lymphocytes may be more sensitive than those of other lymphoid organs. Moreover, the most pronounced repopulation of ...

Lymphocyte exit from lymph nodes and their recirculation into blood is controlled by the sphingolipid sphingosine 1-phosphate (S1P). The cellular receptor mediating lymphocyte exit is S1P(1), one of five S1P receptors. Nonselective agonists for S1P receptors lead to blood lymphocyte count reduction. The effects of ...

Eur J Clin Invest 2011; 41 (10): 1071-1076 ABSTRACT: Background? IFN-? release assays (IGRA), widely used for latent tuberculosis screening prior to anti-TNF-? treatment, are limited by indeterminate results in patients under immunomodulatory (IM) therapy. The aim of our observational study was to delineate factors associated with indeterminate IGRA results. Methods? A total of 190 patients with ...

Study Objectives:Several lines of evidence suggest immune system derangement in obstructive sleep apnea syndrome (OSAS) patients. However, no data exist on the long-term effect of continuous positive airway pressure (CPAP) treatment on systemic immunity. Hence, we sought to evaluate this effect on various immunological parameters in OSAS patients.Design:Prospective case series.Setting:Sleep unit ...

Previous reports have suggested that the immunosuppressive effects of lymphoid irradiation (LI) are related to changes in T cell peripheral blood levels. We prospectively studied 13 patients with Stages I and II Hodgkin's disease before, during, and after a course of LI. Forty to 45Gy delivered to the mantle field in 4-1/2 to 5 weeks was followed by a 3 to 4 week break and 35 to 40 Gy to ...

Thirty two patients with persisting pulmonary sarcoidosis fulfilling defined criteria for treatment were managed according to a standard clinical protocol. In this an attempt was made to achieve and maintain maximal radiographic and physiological improvement with individually titrated doses of corticosteroids. Lavage cell counts, serum angiotensin converting enzyme (SACE) ...

To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in ...

To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in ...

Peripheral blood lymphocyte counts and stimulations by PHA and PPD in vitro were examined before and up to four years after local pre- or post-operative radiation therapy of 99 patients with breast cancer. The patient material was divided into those who remained clinically disease-free during a follow up period of 4.5-7 years and those who relapsed. ...

Peripheral blood lymphocyte and leukocyte levels were monitored in 34 patients with bladder carcinoma before, during, and up to 5 years after radiotherapy. Radiotherapy in doses 6500 to 8500 rads caused a marked decline in the numbers of circulating leukocytes and particularly lymphocytes. In patients clinically free of disease for 5 years, ...

BackgroundIpilimumab is a monoclonal antibody which antagonizes cytotoxic T lymphocyte antigen (CTLA)-4, a negative regulator of the immune system. We report on advanced refractory melanoma patients treated on a compassionate-use trial of ipilimumab at the Memorial Sloan-Kettering Cancer Center.Patients and methodsPatients with advanced refractory melanoma were treated on a ...

Reports of variable response to antiretroviral therapy as indicated by CD4 count has been of concern as facilities for viral load estimation/drug resistance testing is not available everywhere. Hence the present study. was done to assess the magnitude of problem in high prevalence state of Manipur as evidenced by the CD4 T lymphocyte ...

Acute human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV) infection is associated with a massive depletion of memory CD4(+) T lymphocytes in the gastrointestinal tract. To define the dynamics of the CD4(+) T lymphocyte subpopulations in breast milk during acute HIV or SIV infection, lymphocyte populations were ...

Treatment of ponies with a single dose of prednisolone markedly reduced the number of blood lymphocytes. A decrease of the number of eosinophils was also observed. In contrast, the number of neutrophils significantly increased. These profound changes were temporary and returned to the pretreatment level within 48 h. The number of monocytes did not show any of the significant ...

F344/N rats and B6C3F1 mice were exposed to 0, 1, 3, or 6 ppm methyl isocyanate by inhalation for 6 hr on 4 consecutive days. Deaths of rats were observed following 3 ppm exposures, and mice died after exposures to 6 ppm. Deaths appeared to be related to severe respiratory distress. Survivors in high dose groups lost weight initially, then gained weight at rates equal to controls throughout a ...

The predominant immunological finding in HIV⁺ haemophilia patients is a decrease of CD4⁺ lymphocytes during progression of the disease. Depletion of CD4⁺ lymphocytes is paralleled by an increase in the proportion of immune complex-coated CD4⁺ cells. We examined the ...

Purpose: This study examined factors predicting tumor response and progression-free survival in patients with locally advanced cervical carcinoma treated with concurrent chemoradiation (CCRT). Methods and Materials: Medical records of 143 patients with locally advanced cervical carcinoma (International Federation of Gynecology and Obstetrics Stage IB2 to IVA) treated with CCRT were reviewed. ...

Young male mice of the Wistar strain were given totalbody irradiation followed by transfusion marrow cells or lymphocytes. Mice given irradiation with no transfusion served as controls. Marrow cell transfusion resulted in a marked increase of marrow cells, of leukocytes and reticulocytes in the peripheral blood and of nucleated cells in the spheen and thymus. ...

The possibility of stress with associated increases in adrenocortical activity contributing to lymphocyte depletion by extracorporeal irradiation of blood (ECIB) or thoracic duct lymph (ECIL) was studied in adrenalectomized, sham-adrenalectomized, and unoperated calves. No pronounced differences in response, as measured by decreased blood lymphocyte ...

A method is described for measuring the absolute disintegration rate of 87.2-day sulfur-35. ( beta -E/sub max/ = 0.167 Mev). The sulfur to be assayed is converted to sulfur dioxide by ignition of barium sulfate with red phosphorus in an atmosphere of oxygen. The sulfur dioxide is then mixed with methane and counted in an internal gas ...

The design and construction of 4 pi Geiger Mueller Counters, and the technique of absolute radioactivity measurements by beta-particle counting are discussed. Three sources of error are considered: The probability of obtaining one count for each beta-particle passing into the counter volume; Losses due to the absorption of ...