In real-time quantitative PCR studies using absolute plasmid DNA standards, a calibration curve is developed to estimate an unknown DNA concentration. However, potential differences in the amplification performance of plasmid DNA compared to genomic DNA standards are often ignore...
EPA Science Inventory
BackgroundFew reports of the utilization of an accurate, cost-effective means for measuring HPV oncogene transcripts have been published. Several papers have reported the use of relative quantitation or more expensive Taqman methods. Here, we report a method of absolute quantitative real-time PCR utilizing ...
PubMed Central
... Accession Number : ADD344329. Title : Quantitative Real-Time Radioscopial Condition Monitoring at High Radiation-Energy,. Corporate Author : ...
DTIC Science & Technology
... polymerase chain reaction (PCR) assays were developed ... real-time PCR assay, which used ... This sensitive and quantitative assay determination of ...
Real time quantitative RT-PCR is considered as standard for gene expression and mRNA estimate. As a calibration standard, a conserved control gene such as housekeeping genes is commonly used for data normalization and analysis. A significant problem has been observed with increased applications; h...
Technology Transfer Automated Retrieval System (TEKTRAN)
The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols is leading to the publication of papers that may not be technically appropriate. We take the view that ...
... OF MECHANICALLY SCANNED SYSTEMS THE TEST BED INCLUDES A ... PROCESSORS TO ACHIEVE THE NEAR REAL TIME FORMATION OF ...
