In Saccharomyces cerevisiae, the family of ATF/CREB transcriptional regulators consists of a repressor, Acr1 (Sko1), and two activators, Aca1 and Aca2. The AP-1 factor Gen4 does not activate transcription through ATF/CREB sites in vivo even though it binds these sites in vitro. Unlike ATF/CREB activators in other species, Aca1- and ...

BackgroundSaccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been used as host for the expression and secretion of human interleukin-1? (IL1?), using the cell wall protein Pir4 as fusion partner. To achieve high cell density and, consequently, high product yield, BY4741 [PIR4-IL1?] was cultured in an aerated fed-batch reactor, using a ...

... The ACAS [Executive Committee for ACAS, 1995] and ACST [Halliday et al, 2004] trials have demonstrated that CEA plus best medical therapy has a 53% and a 60 ...

Oral supplementation of lactic acid bacteria is a potential approach to the prevention and manipulation of allergic diseases such as atopic dermatitis. Our previous report showed that heat-killed Lactobacillus acidophilus strain L-92 (L-92) possessed anti-allergic properties, although its physiological function in atopic dermatitis has largely remained ...

... Ehe expression for the strain energy is thus reduced to the following simplified form �iff I _STXT (ax�x + ozez) dxdz (17) ... A-.CA. .Iff Wo. 9.93 19 ...

B6.129-Cdkn2atm1Cjs PCR Protocol Strain: B6.129-Cdkn2atm1Cjs Strain Code: 01XG7 Protocol Number: 1 Introductory Comment: Primers:  C018 :   5'-agt aca gca gcg gga gca tgg -3'  C019 :   5'-ttg agg agg acc gtg aag ccg -3'  C020 :   5'-acc aca ctg ctc

The effect of phospholipids on the activity of isoform ACA8 of Arabidopsis thaliana plasma membrane (PM) Ca2+-ATPase was evaluated in membranes isolated from Saccharomyces cerevisiae strain K616 expressing wild type or mutated ACA8 cDNA. Acidic phospholipids stimulated the basal Ca2+-ATPase activity in the following order of ...

Streptococcus macedonicus ACA-DC 198, a bacteriocin producer isolated from Greek Kasseri cheese, was used in a series of in vitro and in vivo experiments in order to evaluate its pathogenic potential. The strain was examined in vitro for haemolytic activity, antibiotic resistance and presence of pathogenicity genes encountered in Streptococcus pyogenes. ...

Alpinia galanga is a rhizomatous herb rich in essential oils and various other significant phytoconstituents. Rapid direct regeneration was obtained from the rhizome explants (15.66?�?0.57 shoots) on MS media supplemented with zeatin at a concentration of 2�mg/l. The callus cultures of A. galanga were initiated from the rhizome explants on MS media ...

The enhancement of industrial antibiotic yield has been achieved through technological innovations and traditional strain improvement programs based on random mutation and screening. The development of recombinant DNA techniques and their application to antibiotic producing microorganisms has allowed yield increments and the design of biosynthetic pathways giving rise to new ...

The chlorated dipeptide L-alanyl-beta-chloro-L-alanine (diACA) is very toxic for Lactococcus lactis. Spontaneous mutants resistant to the dipeptide were isolated from plates. The presence and activities of cell wall-associated proteinase, different peptidases in cell extracts, amino acid transport systems, and di- and oligopeptide transport systems were examined and compared ...

A strain of Bacillus species which produced an enzyme named glutaryl 7-ACA acylase which converts 7 beta-(4-carboxybutanamido)cephalosporanic acid (glutaryl 7-ACA) to 7-amino cephalosporanic acid (7-ACA) was isolated from soil. The gene for the glutaryl 7-ACA acylase was cloned with pHSG298 in ...

Pseudomonas sp. strain SE83 converts cephalosporin C and 7 beta-(4-carboxybutanamido)cephalosporanic acid (GL-7ACA) to 7-aminocephalosporanic acid (7ACA). A DNA library of this strain was constructed in Escherichia coli and screened for the ability to deacylate GL-7ACA to ...

Mixed cultures of strains of opposite sex of the Mucorales produce trisporic acids and other compounds arising from cleavage of ?-carotene, some of which act as signals in the mating process. The genome of Phycomyces blakesleeanus contains five sequences akin to those of verified carotenoid cleavage oxygenases. All five are transcribed, three of them have the sequence traits ...

FVB-Tg(ACTB-ALPP)03Jrst PCR Protocol Strain: FVB-Tg(ACTB-ALPP)03Jrst Strain Code: 01XJ3 Protocol Number: 2 Introductory Comment: Primers:  P017 :   5'-GGA TGG TcT ccA TcT ccT GA -3'  P018 :   5'-AcA TcT GGA cAc TGG GcA TAG -3' Product Sizes:   Primer

FVB-Tg(ACTB-ALPP)02Jrst PCR Protocol Strain: FVB-Tg(ACTB-ALPP)02Jrst Strain Code: 01XH7 Protocol Number: 2 Introductory Comment: Primers:  P017 :   5'-GGA TGG TcT ccA TcT ccT GA -3'  P018 :   5'-AcA TcT GGA cAc TGG GcA TAG -3' Product Sizes:   Primer

FVB-Tg(ACTB-ALPP)01Jrst PCR Protocol Strain: FVB-Tg(ACTB-ALPP)01Jrst Strain Code: 01XH6 Protocol Number: 2 Introductory Comment: Primers: �P017 : � 5'-GGA TGG TcT ccA TcT ccT GA -3' �P018 : � 5'-AcA TcT GGA cAc TGG GcA TAG -3' Product Sizes: ��Primer

FVB-Tg(ACTB-ALPP)01Jrst PCR Protocol Strain: FVB-Tg(ACTB-ALPP)01Jrst Strain Code: 01XH6 Protocol Number: 1 Introductory Comment: Primers:  P015 :   5'-GTc cTc TGG GAT Tcc AAc TGc c -3'  P016 :   5'-GGc ccA AGc cTc AcA cAT TTc -3' Product Sizes:   Primer

FVB;129-Men1tm1.1Ctre PCR Protocol Strain: FVB;129-Men1tm1.1Ctre Strain Code: 01XC7 Protocol Number: 1 Introductory Comment: Primers:  M014 :   5'-ccc AcA Tcc AGT ccc TcT TcA GcT -3'  M015 :   5'-ccc TcT GGc TAT TcA ATG GcA GGG -3'  M016 :   5'-cAT

FVB.129-Trp53tm1Brn PCR Protocol Strain: FVB.129-Trp53tm1Brn Strain Code: 01XC2 Protocol Number: 1 Introductory Comment: Primers:  T008 :   5'-cAc AAA AAc AGG TTA AAc ccA G -3'  T009 :   5'-AGc AcA TAG GAG GcA GAG Ac -3' Product Sizes:   Primer Combination      Product

FVB-Tg(Tyr-rtTA)37Lc PCR Protocol Strain: FVB-Tg(Tyr-rtTA)37Lc Strain Code: 01XB6 Protocol Number: 1 Introductory Comment: Primers:  R009 :   5'-AGG GAA AcA ccT AcT AcT GA -3'  R010 :   5'-ATT ccA AGG GcA TcG GTA -3' Product Sizes:   Primer Combination      Product

B6.129S2-Krastm2Tyj PCR Protocol Strain: B6.129S2-Krastm2Tyj Strain Code: 01BM2 Protocol Number: 1 Introductory Comment: Primers:  K001 :   5'-TGc AcA GcT TAG TGA GAc cc -3'  K002 :   5'-GAc TGc TcT cTT TcA ccT cc -3'  K003 :   5'-GGA GcA AAG cTG

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Streptococcus macedonicus ACA-DC 198, a strain isolated from Greek Kasseri cheese, produces a food-grade lantibiotic named macedocin. Macedocin has a molecular mass of 2,794.76 � 0.42 Da, as determined by electrospray mass spectrometry. Partial N-terminal sequence analysis revealed 22 amino acid residues that correspond with the amino acid sequence of ...

We demonstrate that SrRuO{sub 3} and CaRuO{sub 3} thin films undergo a room temperature structural phase transition driven by the substrate imposed epitaxial biaxial strain. As tensile strain increases, ARuO{sub 3} (A=Ca and Sr) films transform from the orthorhombic phase which is usually observed in bulk SrRuO{sub 3} and CaRuO{sub 3} ...

It has been suggested that all intracellular signaling by cAMP during development of Dictyostelium is mediated by the cAMP-dependent protein kinase, PKA, since cells carrying null mutations in the acaA gene that encodes adenylyl cyclase can develop so as to form fruiting bodies under some conditions if PKA is made constitutive by overexpressing the catalytic subunit. However, ...

Disputes at work are never pleasant and can lead to long term strain on the relationship between district nurses and managers. It is in the best interest of both to deal with any problems openly and fairly. The legislation relating to workplace grievances and claims arising from these to an employment tribunal changed on 6th April 2009. The new framework requires grievances to ...

This study aimed to examine the potential antiviral activity of lactic acid bacteria (LAB) using animal and human intestinal and macrophage cell line models of non tumor origin. To this end, LAB strains selected on the basis of previous in vitro trials were co-incubated with cell line monolayers, which were subsequently challenged with rotavirus (RV) and transmissible ...

An aerobic microorganism with an ability to utilize phenol as sole carbon and energy source was isolated from phenol-contaminated wastewater samples. The isolate was identified as Bacillus amyloliquefaciens strain WJDB-1 based on morphological, physiological, and biochemical characteristics, and 16S rDNA sequence analysis. Strain WJDB-1 immobilized in ...

The aim of the present work was to study the mode of the induction of the biosynthesis of macedocin, the lantibiotic produced by Streptococcus macedonicus ACA-DC 198. Macedocin was produced when the strain was grown in milk but not in MRS or M17 broth. No autoinduction mechanism was observed. Production did not depend on the presence of lactose or ...

The aim of the present work was to study the mode of the induction of the biosynthesis of macedocin, the lantibiotic produced by Streptococcus macedonicus ACA-DC 198. Macedocin was produced when the strain was grown in milk but not in MRS or M17 broth. No autoinduction mechanism was observed. Production did not depend on the presence of lactose or ...

This study examined and sought to clarify several major issues related to Adult Children of Alcoholics (ACA) and the notion that they are at increased risk for developing problems with alcohol. The study involved a questionnaire survey of randomly selected university counseling centers in regard to a variety of ACA issues. A total of 102 questionnaires ...

Restriction endonucleases (RENs) were detected in 650 microbial strains isolated from water columns and bottom sediments of deep rift lakes, Baikal (Russia) and Nyasa (Southeastern Africa). They enzymes included unique (Fan I, Aca I, and Sse 91) and very rare (Bsi I, and Cci N I) species not typical of aquatic ecosystems. Water columns, deep cores, and ...

129S-Aprttm1Jat PCR Protocol Strain: 129S-Aprttm1Jat Strain Code: 01XJ5 Protocol Number: 1 Introductory Comment: Primers:  A006 :   5'-ccA cAA ccT Tcc cTc cTT Ac -3'  A008 :   5'-ATA AGA ccc TGc ccT Tcc TcT AcA cA -3'  A007 :   5'-TGc cTG cTT Gcc

B6.129-Nkx3-1tm1Mms PCR Protocol Strain: B6.129-Nkx3-1tm1Mms Strain Code: 01XF9 Protocol Number: 1 Introductory Comment: Primers:  N005 :   5'-Gcc AAc cTG ccT cAA TcA cTA AGG -3'  N006 :   5'-TTc cAc ATA cAc TTc ATT cTc AGT -3'  N007 :   5'-Gcc AcA

C3.Cg-Tg(RIP1-Tag2)2Dh PCR Protocol Strain: C3.Cg-Tg(RIP1-Tag2)2Dh Strain Code: 01XD6 Protocol Number: 1 Introductory Comment: Primers:  T018 :   5'-GGA cAA Acc AcA AcT AGA ATG cAG -3'  T020 :   5'-cAc cGG AGA ATG GGA AGc cGA A -3'  T019 :   5'-cAG

B6.D2-Tg(RIP1-Tag2)2Dh PCR Protocol Strain: B6.D2-Tg(RIP1-Tag2)2Dh Strain Code: 01XD5 Protocol Number: 1 Introductory Comment: Primers:  T018 :   5'-GGA cAA Acc AcA AcT AGA ATG cAG -3'  T020 :   5'-cAc cGG AGA ATG GGA AGc cGA A -3'  T019 :   5'-cAG

C3H/He-Tg(RIP1-Tag5)5Dh PCR Protocol Strain: C3H/He-Tg(RIP1-Tag5)5Dh Strain Code: 01XD4 Protocol Number: 1 Introductory Comment: Primers:  T018 :   5'-GGA cAA Acc AcA AcT AGA ATG cAG -3'  T020 :   5'-cAc cGG AGA ATG GGA AGc cGA A -3'  T019 :   5'-cAG

B6;129-Nkx3-1tm1Mms PCR Protocol Strain: B6;129-Nkx3-1tm1Mms Strain Code: 01XB3 Protocol Number: 1 Introductory Comment: Primers:  N005 :   5'-Gcc AAc cTG ccT cAA TcA cTA AGG -3'  N006 :   5'-TTc cAc ATA cAc TTc ATT cTc AGT -3'  N007 :   5'-Gcc AcA

B6.129S-Krastm3Tyj PCR Protocol Strain: B6.129S-Krastm3Tyj Strain Code: 01BM3 Protocol Number: 1 Introductory Comment: Primers:  K001 :   5'-TGc AcA GcT TAG TGA GAc cc -3'  K002 :   5'-GAc TGc TcT cTT TcA ccT cc -3'  K003 :   5'-GGA GcA AAG cTG cTA

... in ensuring the health and safety of their employees. The ACA has developed resources to assist patients ... with management to determine who makes decisions regarding employee benefits. Resources to Educate Others about Chiropractic ACA ...

... Accession Number : ADA217683. Title : DURIP - Upgrade of the Meridian ACAS-470 for Toxicological Research. Descriptive Note : Final rept. ...

... 4. TITLE AND SUBTITLE Automatic Air Collision Avoidance Air Collision Avoidance System Auto-ACAS 5a. CONTRACT NUMBER 5b. ...

... Accession Number : ADA427453. Title : Automatic Air Collision Avoidance System (Auto-ACAS). Descriptive Note : Briefing charts. ...

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... OCEAN VOYAGER II OPERATION ACA MED FAU www.fau.edu/AMS/auv. html OCEAN EXPLORER OPERATION ACA LOW FAU " ...

Medically useful semisynthetic cephalosporins are made from 7-aminodeacetoxycephalosporanic acid (7-ADCA) or 7-aminocephalosporanic acid (7-ACA). Here we describe a new industrially amenable bioprocess for the production of the important intermediate 7-ADCA that can replace the expensive and environmentally unfriendly chemical method classically used. The method is based on ...

On starvation, the cellular slime mold Dictyostelium discoideum initiates a program of development leading to formation of multicellular structures. The initial cell aggregation requires chemotaxis to cyclic AMP (cAMP) and relay of the cAMP signal by the activation of adenylyl cyclase (ACA), and it has been shown previously that the Ras protein RasC is involved in both ...

The complete nucleotide sequence including the novel telomere sequence of Streptomyces linear plasmid pSHK1 consists of 187,263-bp, 158 genes, in which 51 genes resemble those of the linear plasmid SCP1 of Streptomyces coelicolor A3(2), and 20 genes encode transposases. Strikingly, the repetitive CRISPRs (clustered regularly interspaced short palindromic repeats) and cas (CRISPR-associated) genes ...

To expand our knowledge on the transplacental carcinogenic potential of inorganic arsenic, pregnant Tg.AC mice received drinking water with 0, 42.5, or 85 ppm arsenite from gestation day 8 to 18. After birth, groups (n = 25) of offspring received topical 12-O-tetradecanoyl phorbol-13-acetate (TPA) (2 microg twice a week) for 36 weeks and were killed; nonskin tumors were assessed. Arsenic increased ...

A variety of extracellular signals lead to the accumulation of cAMP which can act as a second message within cells by activating protein kinase A (PKA). Expression of many of the essential developmental genes in Dictyostelium discoideum are known to depend on PKA activity. Cells in which the receptor-coupled adenylyl cyclase gene, acaA, is genetically inactivated grow well but ...

The aim of this study was to investigate the clinical significance of anticentromere antibody (ACA) among types of antinuclear antibody (ANA) in the properties of oocytes retrieved from infertile women. The rate of metaphase II oocytes or embryo cleavage was significantly decreased in patients with positive ACA compared with patients with negative ...

... and 7-ACA, the affinity and osmosis of antibiotics to specific histionic parts, and modification of the hundreds of newly discovered antibiotics that ...

We examined phenotypic characteristics of six mannitol-fermenting strains of Streptococcus bovis, including two unusual CO2-requiring strains isolated from koala feces. These strains did not grow in air, but grew in air supplemented with CO2 and under reduced oxygen conditions. All six strains ...

By means of procedures designed to address methodological weaknesses of previous research, this study tested hypotheses regarding the presumed greater tendency of adult children of alcoholics (ACA) to be characterized by symptomatology indicative of psychopathology than adult children of nonalcoholics (ACNA). Gender of ACA and gender of alcoholic parent ...

A number of standard bacteriological media with supplements were tested for their ability to support in vitro growth of Ethiopian strains of Borrelia recurrentis. Propagation of 18 out of 21 strains occurred in Trypticase soy yeast broth to which bovine a...

The dependence of supramolecular structure on fractional molecular coverage has been investigated for acridine-9-carboxylic acid (ACA) and the C(60):ACA binary molecular system. The coverage-dependent phase diagram for ACA is first determined from room-temperature STM imaging. At low molecular coverages (theta < 0.4 ML, ML = ...

The most common types include the vibrating wire, strain gauge, quartz-resonator , Bourdon tube, and various capacitance devices. ...