... species (Birch et al. 1985) are the adzuki bean beetle, Callosobruchus chinensis L.; the bean seed beetle, Acanthoscelides obtectus (Say); the cowpea weevil, Callosobruchus maculatus ( ... ...

... Cali, Colombia. CIAT Main insect pest of stored beans and their control. 1986. ... seed beetle, Callosobruchus maculatus. Ecol. Entomol 1986. 11:41�50. ... ...

... trade-offs with age-specific mortality in the beetle Callosobruchus maculatus. ... bean weevil (Acanthoscelides obtectus): the effects of density-dependent ... ...

... used are those proposed by Johnson (1983, 1990). RESULTS KEY TO THE ACANTHOSCELIDES OBTECTUS SPECIES-GROUP 1. Mucro ... ...

segregation of sibling species: the case of bean beetles, Acanthoscelides obtectus Say and A. obvelatus of the bruchid beetle Acanthoscelides obtectus Say, a cosmopolitan pest of beans. Molecular Ecology, 14, 1015 and community structure. 2. Acanthoscelides obtectus and A. ...

Jun 16, 2011 � The eggs and neonate larvae of Acanthoscelides obtectus Say (Coleoptera: Bruchidae) were the only accessible immature stages among all prey ...

Effects of ionizing radiations were studied in Acanthoscelides obtectus. Chorion covered ovocytes stored in lateral oviducts were more sensitive than young growing oocyte. On other hand, studies of fine structure on ovarioles of treated females show that damage to oocyte membrane can explain vitellogenesis perturbations. (FR)

Plant defensins are small cysteine-rich proteins commonly synthesized in plants, encoded by large multigene families. Most plant defensins that have been characterized to date show potent antifungal and/or bactericidal activities. This report describes VuD1, an unusual defensin that is able to inhibit insect-pest alpha-amylases. VuD1 was cloned from cowpea (Vigna unguiculata) seeds and expressed ...

Cysteine proteinases from larvae of the common bean weevil, Acanthoscelides obtectus (Coleoptera: Bruchidae), were isolated by ion exchange affinity chromatography on a CM-Cellulose column and used to select mutant cystatins from a library made with the filamentous M13 phage display system. The library contained variant cystatins derived from the nematode ...

The cowpea weevil Callosobruchus maculatus is one of the major pests of Vigna unguiculata cowpea. Digestion in the cowpea weevil is facilitated by high levels of cysteine and aspartic acid proteinases. Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves against attack by insects. In this work, a trypsin inhibitor (ApTI) ...

Acanthoscelides obtectus Say is a bruchid species of Neotropical origin, and is specialized on beans of the Phaseolus vulgaris L. group. Since the domestication and diffusion of beans, A. obtectus has become cosmopolitan through human-mediated migrations and is now a major pest in bean granaries. Using phylogeographic methods applied ...

The influence of both predator density and elapsed time between initial infestation and introduction of predators were determined for suppression of bruchids infesting stored grain legumes by Xylocoris flavipes (Reuter)(Hemiptera: Anthocoridae). Suppression of Acanthoscelides obtectus approached er...

Bean weevil (Acanthoscelides obtectus (Say)) females were found to use seeds (discrete resource patches) differentially when different sizes were offered in multiple-choice tests. Females, either as a group or as individuals, laid significantly (two to six times) more eggs on large seeds than on those of five times smaller mass. In contrast, seed shape ...

Choice of the targeted host developmental stage, regulation of parasitoid numbers released and introduction of food supplements are operational factors with a potential to influence the level of biological control. In a closed laboratory storage system maintained over two generations of the host, the impact of these three parameters on the control potential of the parasitoid Dinarmus basalis ...

We investigated the additive, dominance and epistatic genetic effects underlying differentiation in oviposition preference between two populations of the seed beetle Acanthoscelides obtectus evolved in the laboratory for 102 generations on bean and chickpea seeds. We reared and tested females on each of two host legumes. The populations differed in mean ...

Acanthoscelides obtectus is a devastating storage insect pest capable of causing severe bean crop losses. In order to maintain their own development, insect pest larvae feed continuously, synthesizing efficient digestive enzymes. Among them, cysteine proteinases (CPs) are commonly produced as inactive precursors (procysteines), requiring a cleavage of the ...

Wheat alpha-amylase inhibitors represent an important tool in engineering crop plants against bean bruchids. Because Acanthoscelides obtectus is a devastating storage bean insect-pest, we attempted to purify and characterize its gut alpha-amylases, to study their interaction with active proteinaceous inhibitors. Two digestives alpha-amylases (AoA1 and ...

[reaction: see text] A formal total synthesis of the sex attractant of male dried bean beetle, methyl (R,E)-(-)-tetradeca-2,4,5-trienoate, was achieved by a new efficient route utilizing the Pd-catalyzed asymmetric allene synthesis reaction. It was found that the atropisomeric biaryl bisphosphine (R)-segphos showed better enantioselectivity than (R)-binap in the Pd-catalyzed reaction for preparing ...

BackgroundWe report on the probable horizontal transfer of a mitochondrial gene, cytb, between species of Neotropical bruchid beetles, in a zone where these species are sympatric.The bruchid beetles Acanthoscelides obtectus, A. obvelatus, A. argillaceus and Zabrotes subfasciatus develop on various bean species in Mexico. Whereas A. ...

This study investigates the effects of different temperatures on the total carbohydrate, lipid and protein amounts of Acanthoscelides obtectus Say, which is a common cereal pest. Studies have been carried out under laboratory conditions at 20 +/- 2 degrees C, 30 +/- 2 degrees C and 60 +/- 5% relative humidity. No specific photoperiodic regimen has been ...

This study was conducted to determine the insecticidal activity of essential oils from oregano, Origanum onites L. (Lamiales: Lamiaceae), savory, Satureja thymbra L. (Lamiales: Lamiaceae), and myrtle, Myrtus communis L. (Rosales: Myrtaceae) against three stored-product insects. Essential oils from three species of plants were obtained by Clevenger-type water distillation. The major compounds in ...

Alpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new alpha-amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 ...

The biochemical interaction between fungal pathogens and their insect host epicuticle was studied by examining fungal hydrocarbon degrading ability. As a contact insecticide, entomopathogenic fungi invade their host through the cuticle, covered by an outermost lipid layer mainly composed of highly stable, very long chain structures. Strains of Beauveria bassiana and Metarhizium anisopliae ...

The tropical forage legume Clitoria ternatea (L.) has important agronomic traits such as adaptation to a wide range of soil conditions and resistance to drought. It is resistant to a number of pathogens and pests. These important traits gave us reasons to look more closely at the plant. A highly basic small protein was purified from seeds of C. ternatea to homogeneity by using ultrafiltration with ...

Plant cystatins show great potential as tools to genetically engineer resistance of crop plants against pests. Two important potential targets are the bean weevils Acanthoscelides obtectus and Zabrotes subfasciatus, which display major activities of digestive cysteine proteinases in midguts. In this study a cowpea cystatin, a cysteine proteinase inhibitor ...

ABSTRACT A hydrophobic 19.7-kDa amylase inhibitor (AI) was purified from corn kernels by 95% ethanol extraction and anionic exchange chromatography. The AI has an isoelectric point of 3.6 and was very stable at different pH values and high temperatures, maintaining 47.6% activity after heating to 94 degrees C for 60 min. Amino acid analysis indicated high valine, leucine, glycine, alanine, and ...

... 955 High Prevalence of Wolbachia in the Azuki Bean Beetle Callosobruchus chinensis (Coleoptera, Bruchidae)Natsuko Kondo1, Masaka...

... lifetime fecundity and female mortality of the bruchid beetle, Callosobruchus ... maculatus and Callosobruchus rhodesianus (Coleoptera: Bruchidae). ... ...