Osteoblasts and endothelium constitute functional niches that support hematopoietic stem cells (HSC) in mammalian bone marrow (BM) 1,2,3 . Adult BM also contains adipocytes, whose numbers correlate inversely with the hematopoietic activity of the marrow. Fatty infiltration of hematopoietic red marrow follows irradiation or chemotherapy ...

Mice were given sub-lethal (200-600 cGy) or near-lethal (800 cGy) whole body irradiation and the effect of injecting syngeneic marrow on subsequent hematopoietic recovery was studied. Marrow cell injection enhanced erythropoietic recovery after sub-lethal irradiation as reflected in hematocrit values and rate of ...

It was previously demonstrated, by means of fixed tissue sections, that cell-free spleen extracts are capable of accelerating the general radiation recovery of spleen and bone marrow. For quantitation of various bone marrow cell types, further work using smear preparations rather than sections was conducted. ...

The present investigation is a continuation of efforts to characterize the radioprotective potential of priming with vincristine (VcR). In this study, the postirradiation recovery kinetics of the marrow's hematopoietic stem cell, progenitor cell, and stromal cell compartments were monitored following exposure to a range of sublethal radiation ...

Accelerated post-irradiation recovery of hematopoietic marrow has been reported following treatment with lithium (Li) or vincristine (VcR). Because these two agents appear to exert their effects on different, albeit overlapping, hematopoietic populations, it was felt that combining them might lead to a wider spectrum of enhanced ...

Etoposide inhibits the activity of topoisomerase II, and possesses radiosensitizing effects on cultured cells. The authors showed that pretreatment of mice with etoposide 1 day before whole-body irradiation (WBI) had a protective effect against radiation-induced bone marrow death. The number of endogeneous colony-forming units (CFU-S) surviving in WBI mice was significantly ...

Administration of olive oil within an hour before or after whole-body x irradiation of male mlce at 625 r significantly increased the survival-rate when compared with irradiated untreated mice. The effects of irradiation on bone- marrow, spleen, and thymus of irradiated, and oil-treated irradiated, male mice at different timeintervals after irradiation have been ...

Bone marrow-derived stem cells have the ability to migrate to sites of tissue damage and participate in tissue regeneration. The number of circulating stem cells has been shown to be a key parameter in this process. Therefore, stimulating the mobilization of bone marrow stem cells may accelerate tissue regeneration in various animal ...

Injection of mouse bone marrow preserved in 15% glycerol at --80 deg C for six weeks was shown to be quantitatively similar to injections of fresh marrow in inducing recovery in mice after lethal irradation. The effect of the preservation procedure on subsequent rates of DNA synthesis was determined in vitro for both preserved ...

... Descriptors : *HEMATOPOIETIC SYSTEM, *RADIATION EFFECTS, *ACCELERATION TOLERANCE, SPACE ENVIRONMENTS, BONE MARROW ...

The recovery of bone marrow cells irradiated in vitro and transplanted into reticuloendothelial system (RES)-blockaded host mice was studied. Mice treated with carbon particles provided a favorable environmental effect on the recovery of irradiated spleen colony-forming cells. The shift of the survival curve of colony-forming units ...

The effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on hematopoietic reconstitution after autologous bone marrow transplantation was evaluated in a primate model. Animals were given a continuous intravenous infusion of recombinant human GM-CSF for several days both before and after transplantation or only after the transplant procedure. ...

Megakaryocytic recovery was studied on the 9th postirradiation day in spleen-extract-treated guinea-pigs and in saline controls following 650 r (LD/sub 75/20/) whole-body Co/sup 60/ gamma radiation. An accelerated regeneration of these cells was found in the extract-treated group. Centers of active megakaryocytic regeneration were observed in the ...

Studies were conducted on the appearance of cells in recipient bone marrow with chromosome markers after bone marrow transfusion to recipients that had different treatments. Investigators tried to replete the bone marrow CFV spleen at various times after ...

Radioinduced histological changes were studied in the spleen and bone marrow of guinea pigs that were exposed to the LD/sub 75/20/ (650 r) of Co/sup 60/ gamma radiation and had received homologous or heterologous cell-free spleen extracts after irradiation. In both organs, postirradiation treatment with the spleen extracts accelerated the ...

The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate hemopoietic regeneration and ...

A 15-year-old boy with rhabdoid sarcoma received chemotherapy, which was followed by bone marrow depression, massive nosebleeds and, finally, hematologic recovery. On both hepatobiliary and renal scintigraphy, prominent vertebral activity was present in early images. Correlation with his clinical course suggests that the findings reflect hyperemia due to ...

Previously reported results of low-temperature preservation of bone marrow with 10% polyvinylpyrrolidone (PVP) were re-evaluated on the radiation protection basis. The lethally irradiated mice show 80% recovery after the transplantation of PVP-preserved isologous bone marrow, when compared to the recovery ...

In a series of studies designed to extend our understanding of interleukin-2 (IL-2) and to study the effect of biologic response modifiers on bone marrow, we observed that administering recombinant human (rH) IL-2 to normal mice resulted in an increase in the frequency of colony-forming units-culture (CFU-C) in bone marrow. In addition, rH IL-2 was able to ...

The repair of large nonunions in long bones remains a significant clinical problem due to high failure rates and limited tissue availability for auto- and allografts. Many cell-based strategies for healing bone defects deliver bone marrow stromal cells (BMSCs) to the defect site to take advantage of the inherent osteogenic capacity of this cell type. However, many factors, ...

The repair of large nonunions in long bones remains a significant clinical problem due to high failure rates and limited tissue availability for auto- and allografts. Many cell-based strategies for healing bone defects deliver bone marrow stromal cells (BMSCs) to the defect site to take advantage of the inherent osteogenic capacity of this cell type. However, many factors, ...

Qualitative and quantitative histological snd histochemical evidence is presented which indicates that cell-free saline spleen extracts modify radiation induced changes in the spleen and bone rnarrow of guinea pigs following exposure to an LD/sub 75///sub 20/ of Cow gamma radiation. Post-irradiation treatment with homologous or heterologous (mouse) spleen extract ...

... NMDP's critical information technology infrastructure must ... to update the disaster recovery plan based ... period the Business Continuity Action Guide ...

This study was aimed to investigate the effect of intra-bone marrow (IBM) injection of allogeneic mesenchymal stem cells (MSCs) on reconstruction of bone marrow MSCs (BM-MSCs) in rats that received hematopoietic stem cell transplantation (HSCT), and to detect the donor MSCs in the hosts for clarifying the effect mechanism of donor MSCs. Wistar female rats ...

We have attempted to evaluate in vivo effects of granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on acute radiation hematopoietic injury in mice. BDF1 mice, irradiated with 7.5-Gy x-rays, were injected i.p. twice daily for 10 days with 10(5) U recombinant human G-CSF, 3.75 x 10(5) U recombinant murine GM-CSF, or a combination of both. ...

... The amount of injury to the bone marrow appears similar when two ... the recovery patterns was noted after a conditioning dose under hypoxic and air ...

Five patients with acute leukemia were treated by totalbody irradiation and marrow infusion. Dose rates ranged from 0.5 to 2 r per minute. The first patient received l,460 r followed by marrow from her mother. Marrow recovery occurred, but the patient died of infection after 21 days. The second patient ...

The human bladder carcinoma cell line 5637 produces hematopoietic growth factors (granulocyte and granulocyte/macrophage colony-stimulating factors (G-CSF and GM-CSF)) and hemopoietin 1, which synergizes with CSFs to stimulate colony formation by primitive hematopoietic stem cells in 5-fluorouracil-treated mouse bone marrow. Molecular and functional properties of hemopoietin 1 ...

Long-term functional capacities of marrow cell lines were defined by competitive repopulation, a technique capable of detecting a small decline in repopulating abilities. There was little or no difference between cells from old and young donors, but a single serial transplantation caused a large decline in repopulating ability. Varying the numbers of ...

To study radiobiological effects and drugs, which can modify radiation injury, has an importance if we would like to avoid harmful effects of radiation due to emergency situations or treat patients with malignant diseases by radiotherapy. During the long treatment schedules patients may be treated by not only anticancer but many other drugs because of accompanying diseases. These drugs may also ...

To study radiobiological effects and drugs, which can modify radiation injury, has an importance if we would like to avoid harmful effects of radiation due to emergency situations or treat patients with malignant diseases by radiotherapy. During the long treatment schedules patients may be treated by not only anticancer but many other drugs because of accompanying diseases. These drugs may also ...

... Abstract : A 3 MeV electrostatic accelerator has been modified for high electron current operation using beam recovery. ...

Marrow was removed from the femurs. f dogs and frozen in 15% glycerol at 1 deg C per minute. Marrow from one dog was stored at -80 deg C and from the other at -180 deg C for a period of 14 months. Each dog was then given 1200 r of wholebody irradiation followed by infusion of his own thawed marrow. ...

... Accession Number : ADA087626. Title : Options for Accelerating Economic Recovery after Nuclear Attack. Volume 3. Descriptive Note : Final rept. ...

It appears that any dose of a single whole-body radiation that would allow survival of the organism with marrow grafting would also allow recovery of the remaining marrow elements without need for marrow grafting if careful substitution therapy is used. There appears to be no prima facie case for ...

The postradiation repair of the hemopoietic function in human beings and of changes observed in bone marrow and peripheral blood is described. The processes of damage and recovery of bone marrow from acute radiation sickness induced by external radiation are described, based on observations of 77 samples made from the first to the ...

Bone marrow transplanting accelerates the onset of remission. As for the length of it, it is still early to form any conclusions in view of the short period of observation. One patient discharged from the clinic with clinical and blood remission was again...

Pretreatment of mice with recombinant murine (rM) colony-stimulating factor-granulocyte-macrophage (CSF-gm) or recombinant human (rH) CSF-g provides partial protection from the lethal effects of ionizing radiation or the alkylating agent cyclophosphamide (CTX). In addition, these agents can significantly prolong survival if administered following lethal doses of irradiation or CTX. To induce ...

The aim of this study was to investigate the effect of mesenchymal stem cells (MSCs) on cell cycle and apoptosis of thymus, spleen and bone marrow cells in mice totally irradiated with sublethal dose, and to explore its mechanisms. BALB/c mice irradiated with 5.5 Gy 60Co gamma-ray were randomly divided into control group and MSC group. Mice in MSC group were infused with 0.4 ...

Natural killer (NK) cells are reported to have an important role in the resistance of lethally irradiated recipients to bone marrow transplantation (BMT). Therefore, we investigated the effects of recipient NK depletion on survival, chimerism, and hematopoietic reconstitution after lethal irradiation and the transplantation of limiting amounts of T-cell-deficient bone ...

Data in this report determined the effect of a single injection of recombinant interleukin 1 alpha (rIL-1) prior to irradiation of B6D2F1 mice on the recovery of colony-forming cells (CFC) at early and late times after sublethal and lethal doses of radiation. Injection of rIL-1 promoted an earlier recovery of mature cells in the blood and CFC in the bone ...

Data in this report determined the effect of a single injection of recombinant interleukin 1 a (rIL-1) prior to irradiation of B6D2F1 mice on the recovery of colony-forming cells (CFC) at early and late times after sublethal and lethal doses of radiation, Injection of rIL-1 promoted an earlier recovery of mature cells in the blood and CFC in the bone ...

The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate hemopoietic regeneration and ...

The stimulation of radiation recovery by bone marrow injections and the immunological background for the study of bone marrow transplantation in mammals are reviewed. (C.H.)

5-Fluouracil (5-Fu) targeting of cycling hematopoietic cells results in bone marrow (BM) suppression. Interleukin 1 receptor antagonist (IL-1Ra) is a cytokine that competitively blocks the binding of interleukin 1 (IL-1) to its receptor. Unlike the supporting role of IL-1 less is known for the role of IL-1Ra in hematopoiesis. Here, we demonstrate that IL-1Ra expression in BM ...

Bone marrow stromal fibroblasts (CFU-F) normally do not exchange bone marrow sites in vivo. Restitution of the CFU-F after radiation damage is primarily recovery by the local fibroblasts from potentially lethal damage. Migration of stromal fibroblasts from shielded sites to an irradiated site makes a minimal contribution, if any, to ...

... exploration of alternative stem cell sources, acceleration ... targeting important transplant-related complications ... without a matched bone marrow donor ...

Nineteen rhesus monkeys were irradiated at doses of 800 to 890 rads of x rays and each transfused with nucleated cells obtained from its own marrow cavity. Seven treated in this way and not routinely treated with antibiotic died, though one had a largely re-populated marrow. The remaining twelve were treated routinely with penicillin and ...

The case of an infant with acute leukemia following a tissue dose of 864 r of whole-body irradiation, midline air dose 1082 roentgens (36 r per hr), is described. The patient was kept in good clinical condition by isolation, supportive therapy, antibiotics, and platelet transfusions. Multiple infusions of fetal hematopoietic tissue and one infusion of adult marrow ...

In vitro colony-forming cells (CFU/sub c/) were evaluated in preleukemic and leukemic AK mice. Increased concentrations of CFU/sub c/ were found in normal appearing marrow of superinfected animals 2 to 3 wk prior to the onset of lymphoma. CFU/sub c/ were present in marrows of mice with virus-accelerated, spontaneous, and transplanted ...

A saline suspension of bone marrow from adult guinea pigs was injected intravenously into another adult guinea pig that had been given 550 r of whole- body x irradiation. In order to identify the injuries to the injected cells, the bone marrow elements to be transfused were labeled with radioactive phosphorus. On the 5th day, after transfusion, ...

Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) is a potential therapy for cerebral ischemia. Although BMSCs-induced angiogenesis is considered important for neurological functional recovery, the neurorestorative mechanisms are not fully understood. We examined whether BMSCs-induced angiogenesis enhances cerebral tissue perfusion and ...

Thrombopoietic cells may differentially promote or inhibit tissue vascularization by releasing both pro- and antiangiogenic factors. However, the molecular determinants controlling the angiogenic phenotype of thrombopoietic cells remain unknown. Here, we show that expression and release of thrombospondins (TSPs) by megakaryocytes and platelets function as a major antiangiogenic switch. TSPs ...

The use of recombinant human granulocyte-stimulating factor (G-CSF) has been shown to effectively accelerate granulocytic recovery after autologous bone marrow transplantation (BMT) in adults. The experience, however, is limited in children. We evaluated the hematopoietic reconstitution in 41 consecutive children undergoing autologous ...

Fifteen patients with Philadelphia chromosome (Ph)-positive chronic myelogenous leukemia (CML) who were ineligible for allogeneic bone marrow transplantation (BMT) or alpha-interferon therapy were included in this study. Eight patients were in the first late chronic phase, five were in the second chronic phase, one was in the accelerated phase, and one was ...

Hematoporphyrin (1,3,5,8-tetramethyl-2,4-bis(hydroxyethyl)-porphin-6,7-dipropionic acid dihydrochloride derivative) (HPD) is a compound that was studied in a number of laboratories because of its cytocidal activity after activation by light. Modification of immune function seen during the photochemotherapeutic studies prompted attempts to determine the effect of HPD on the immune and hemopoietic ...

... 3: NMDP's critical information technology infrastructure must ... IIA.3.1 Aim 1: IS Disaster Recovery ... Period 3 Activity: � Business Continuity Planning: ...

Following space flight, spinal disc and muscle recovery may be delayed, muscle damage may be greater, back pain may be exacerbated and bone marrow response ...

... This recovery indicates that lymphocytic cells probably could not give rise to the other two cell types (function as a stem cell) through a ...

Copper (II)(3,5-diisopropylsalicate)/sub 2/ (Cu(II) (3,5-dips)2) afforded significant protection when administered subcutaneously 3 hr before whole body irradiation. At 800 Rad (170 Rad/min) using a cesium-137 irradiator, 86% of mice receiving 80 mg/kg of the copper complex survived while only 40% of irradiated mice receiving only the polyvinyl alcohol vehicle survived. Seven days after ...

We report that the bone marrow (BM) stroma-released LL-37, a member of the cathelicidin family of antimicrobial peptides, primes/increases the responsiveness of murine and human hematopoietic stem/progenitor cells (HSPCs) to an ?-chemokine stromal-derived factor-1 (SDF-1) gradient. Accordingly, LL-37 is upregulated in irradiated BM cells and enhances the chemotactic ...

Gray Collie-normal bone marrow transplantation chimeras showed normal coloration of the hair coat on tails and several other areas 2 years after successful transplantation of bone marrow to correct cyclic neutropenia of the Gray Collie syndrome.ImagesFigures 1-2

This report shows the early detection of reactivation of chronic Chagas' disease (CCd) in a 27-year-old man with chronic myelogenous leukemia undergoing allogeneic bone marrow transplantation (ABMT). Pre-emptive therapy with benznidazole during a period of 7 weeks led to a rapid recovery of the patient, who remains free of parasitemia 2 years after the ...

Hematopoietic depression and subsequent susceptibility to potentially lethal opportunistic infections are well-documented phenomena following radiotherapy. Methods to therapeutically mitigate radiation-induced myelosuppression could offer great clinical value. In vivo studies have demonstrated that interleukin-6 (IL-6) stimulates pluripotent hematopoietic stem cell (CFU-s), granulocyte-macrophage ...

Polyribo-cytidylic and uridylic acids possess recovery properties; such properties are not very significant for polyriboadenylic acid and nonexistent for polyriboguanylic acid. A correlation is apparent between the recovery activity of these acids and their affinity for nucleases, this fact being confirmed by the very large ...

The Intraperitoneal injection of lead acetate in a daily doze of 50 mg/kg for 10 days led to the development of myelosuppression of the bone-marrow pool in mice. On the background of this immunodepressive syndrome, the treatment with B1V-309 azaheterocycle during 3 days reliably accelerated the regeneration of neutrophiles, lymphocytes, and monocyte cells ...

Medications that can mitigate against radiation injury are limited. In this study, we investigated the ability of recombinant human growth hormone (rhGH) to mitigate against radiation injury in mice and nonhuman primates. BALB/c mice were irradiated with 7.5 Gy and treated post-irradiation with rhGH intravenously at a once daily dose of 20 microg/dose for 35 days. rhGH protected 17 out of 28 mice ...

Medications that can mitigate against radiation injury are limited. In this study, we investigated the ability of recombinant human growth hormone (rhGH) to mitigate against radiation injury in mice and nonhuman primates. BALB/c mice were irradiated with 7.5 Gy and treated post-irradiation with rhGH intravenously at a once daily dose of 20 �g/dose for 35 days. rhGH protected 17 out of 28 mice ...

We compared the effects of hematopoietic stem cell and marrow stromal cell transplantation for spinal cord injury in mice. From green fluorescent protein transgenic mouse bone marrow, lineage-negative, c-kit- and Sca-1-positive cells were sorted as hematopoietic stem cells and plastic-adherent cells were cultured as marrow stromal ...

Macrophage colony-stimulating factor (M-CSF) is active in the late stages of monocyte maturation, activates mature monocyte-macrophages and enhances their production of various other cytokines. We have examined the effects of a 21 d course of escalating doses of M-CSF purified from human urine (hM-CSF) on recovery following autologous bone marrow ...

Collection, storage, and subsequent infusion of bone marrow are described, and successful results of infuslon of marrow cells in heavily irradiated (2,400 r total dose) patients are reported. In each case, the patlent's bone marrow was severely hypoplastic at the time of infusion. No patient suffered ill effects ...

The effect of cooling rate on recovery of human and murine hemopoietic precursor cells was studied. In the presence of 10% Me2SO, a cooling rate of 7 degrees C/min from -4 to -30 degrees C was optimal for recovery of both human and murine precursor cells which give rise to colonies in diffusion chambers implanted in mice (CFU-DG). Cooling of human ...

Based on transplantation studies with bone marrow cultured under various conditions, a role of interleukin 11 (IL-11) in the self-renewal and/or the differentiation commitment of hematopoietic stem cells has been indicated. To better evaluate the in vivo effects of IL-11 on stem/progenitor cell biology, lethally irradiated mice were serially transplanted with bone ...

Damage in the precursors of blood cells due to incorporation of tritiated thymidine was evaluated in vivo. The parameter measured was the speed of recovery of peripheral blood cells after isologous marrow transplantation in irradiated mice. Donor animals received a total of either 20 or 40 mu C of H/ sup 3/-thymidine per gm of body weight. A ...

Data presented in this report describe countercurrent centrifugal elutriation (CCE) recovery profiles of hematopoietic colony-forming cells (CFC) in marrow from normal and 5-fluorouracil(5-FU) treated mice. Of the total nucleated cells, 75%-95% were recov...

HOME RESEARCH Research at SLAC Accelerator Physics Astrophysics and Cosmology Elementary Particle Physics Materials and Nanoscience Molecular Environmental Science Structural...

In this paper, the electron beam diagnostics developed for recirculating electron accelerators will be reviewed. The main novelties in dealing with such accelerators are: to have sufficient information and control possibilities for the longitudinal phase ...

The purpose of the present study was to investigate the efficacy of a liquid culture filtrates of the entomogenous fungus Paecilomyces tenuipes (PTCF) and its main active glycoprotein-enriched (PGF) fraction against hematotoxicity in mice treated with 5-fluorouracil (5-FU). Oral administration of PTCF (100 mg/kg/d) for 7 consecutive days after 5-FU injection significantly suppressed reductions in ...

Natural killer (NK) cells are reported to have an important role in the resistance of lethally irradiated recipients to bone marrow transplantation (BMT). Therefore, we investigated the effects of recipient NK depletion on survival, chimerism, and hematopoietic reconstitution after lethal irradiation and the transplantation of limiting amounts of T-cell-deficient bone ...

After a single total-body x-irradiation of 600 r erythroblasts, granulocytes, and lymphocytes were markedly damaged and most of the cells disappeared within 24 hr. Hematopoietic tissues became aplastic within 72 hr. The lymphopoietic tissues were damaged more severely in the lymph nodes than in the spleen. Erythroblasts and granulocytes in the bone marrow and in the ...

A total of 22 patients with leukemia have undergone allogeneic bone marrow transplantation (BMT) by the Quebec Co-operative Group for Marrow Transplantation from 1980 to 1982. All patients received 900 cGy total body irradiation (TBI), in a single fraction, on the day preceding BMT. The first 11 patients were treated on a cobalt unit at a constant dose ...

with the intent of the Recovery Act, the purpose of this program is to promote job creation and economic development along with accelerating the pace and achievement of...

... The primary focus on this study was to measure recovery latency periods for both the absolute and relative incapacitation period following a G-LOC ...

This communication reports the results of studies designed to investigate the ability of recombinant murine interleukin-1 (rIL-1) to enhance the recovery of hematopoiesis following administration of sub-lethal whole body irradiation (2 Gy). Mice were administered rIL-1 (100 and 500 units) i.p. Twenty-four hours later these mice were administered 2 Gy radiation. Irradiated ...

Twerty cases of aplastic anemia were treated with infusions of homologous bone marrow, and their course compared with the results obtained in 17 patients treated with measures other than marrow infusions. No differences in the overall survival were found between the group treated with bone marrow and the control group. ...

The purpose of this study was to investigate the efficacy of four different Japanese and Chinese herbal prescriptions, Ren-Shen-Yang-Rong-Tang (Ninjin�yoeito, NYT), Chai-Hu-Gui-Zhi-Gan-Jiang-Tang (Saikokeishikankyoto, SKKT), Si-Jun-Zi-Tang (Shikunshito, SKT) and Si-Wu-Tang (Shimotsuto, SMT), which are traditionally used for anemia and fatigue, against hematotoxicity in mice treated with ...

In this report, we present the results of an experimental investigation of the use of a ferrite loaded cavity for electron beam energy recovery and auto-acceleration. The efficiency in the energy recovery mode is shown to be limited by the formation of vi...

Severe and prolonged cytopenias represent a considerable problem in clinical stem cell transplantations. Cytokine-induced ex vivo expansion of hematopoietic stem and progenitor cells has been intensively explored as a means of accelerating hematopoietic recovery following transplantation but have so far had limited success. Herein, overexpression of D-type ...

Bone marrow aplasia was induced in rats by whole body lethal irradiation (1,000 rads by x-ray), and rats died of irradiation injury within 7 days. Correlative studies at light (LM), transmission (TEM) and scanning electron microscopy (SEM) demonstrated swelling of endothelial and reticular cells and hemorrhage due to detachment of sinus endothelial cells on days 1 and 2. With ...

... ANIMALS, STRESS(PHYSIOLOGY), ACCELERATION, CENTRIFUGES, MORTALITY RATE, ANTICOAGULANTS, RECOVERY, SURVIVAL ...

... in this report utilize anatomical, metabolic or biochemical measurennts to analyze the mechanism(s) of amphetamine accelerated recoveri fron the ...

The capacity of NZB stem cells to proliferate in vivo was evaluated in two systems which required repopulation of peripheral organs. In both types of depletion systems, stem-cell repopulation after cyclophosphamide treatment or adoptive transfer repopulation in lethally irradiated hosts, it was found that NZB stem cells were hyperproliferating. The increase in proliferating cells was most ...

We describe the clinical course of a 16 year old girl with aplastic anemia who was treated by syngeneic bone marrow transplantation. Engraftment was not obtained by simple infusion of bone marrow without immunosuppression. The patient received a high-dose cyclophosphamide and thoracoabdominal irradiation, followed by second marrow ...

Stumptail monkeys (Macaca speciosa) received lethal whole body radiation. Autologous bone marrow injection resulted in survival of the majority of the animals. Treatment with Deca-Durabolin, an anabolic steroid, caused more rapid recovery of colony-forming cell numbers in the bone marrow than in control animals. Both the ...

Stumptail monkeys (Macaca speciosa) received lethal whole-body radiation. Autologous bone marrow injection resulted in survival of the majority of the animals. Treatment with Deca-Durabolin, an anabolic steroid, caused more rapid recovery of colony-forming cell numbers in the bone marrow than in control animals. Both the ...

For an energy-efficient accelerator system to be used for a free-electron laser, the stability of an energy-recovery system utilizing a bridge coupler placed between the accelerator and the decelerator is studied numerically. Energy is recovered by recirc...

When hematopoietic stem cells (HSCs) were administrated by intravenous infusion (IV), most of them were trapped in some nonhematopoietic organs as like lungs that had abundant blood capillaries. Only a small fraction of injected cells could home to the bone marrow, which reduced the engraftment of HSCs. The purpose of intra-bone marrow (IBM) ...

Hypertransfusion can enhance recovery from neutropenia in certain clinical and experimental situations. We have studied the pattern of myeloid recovery in mice hypertransfused after receiving 350 rads whole body irradiation. Both hypertransfused and control groups showed the degenerative phase, abortive rise, and regenerative phase that has been described ...

We previously demonstrated that the electrical state of the skin surface influences epidermal permeability barrier homeostasis. At the interface between different materials, electrons are localized heterogeneously and induce electrical potential. In the present study, we evaluated the effects of metals on the barrier recovery. When we put pure gold plate on skin immediately ...

This report examines the links between selected provisions of the Economic Recovery Tax Act (ERTA) of 1981 and a subset of economic decisions made in the private sector. The ways in which the Accelerated Cost Recovery System (ACRS), the liberalized invest...

Total body irradiation followed by bone marrow transplantation was found to be an effective treatment for adjuvant arthritis induced in rats. This treatment is most effective when applied shortly after the clinical manifestation of arthritis--i.e., 4-7 weeks after administration of Mycobacterium tuberculosis. Transplantation of bone marrow at a later stage ...

... skin wound healing in a rat model of diabetes. A skin wound was made on the backs ... and age-matched control rats 60 days after diabetes was induced by a single injection of streptozotocin. ... ...

AbstractThe risk of genotoxicity of retroviral vector-delivered gene therapy targeting hematopoietic stem cells (HSCs) has been highlighted by the development of clonal dominance and malignancies in human and animal gene therapy trials. Large-animal models have proven invaluable to test the safety of retroviral vectors, but the detection of clonal dominance may require years of follow-up. We ...

Recently, a 22-year-old Caucasian female was referred to our Hospital two days post-partum. She had been feeling unwell during the last few days of her pregnancy and complained of multiple aches and pains, worst in the abdomen and lower back. Her admission platelet count was severely depressed and a bone biopsy showed extensive marrow necrosis with viable bony trabeculae. ...

-term engraftment of autologous blood stem cells. Bone Marrow Transplant. 2000; 26: 1299-1304. 41. Uyl-de Groot CA and Marrow Transplantation. Haematopoietic stem cell transplantation (HSCT) in Europe 2002. Changes of peripheral blood stem cells: effects on hematopoietic recovery after high-dose chemotherapy. Bone ...

The replacement of bone marrow in rabbits subjected to high dose-levels of irradiation has been investigated. Since doses large enough to destroy the marrow of rabbits cause fatal damage to other organs, colloidal Au/sup 198/ was used as a source of selective irradiation of bone marrow and other hematopoietic tissues, followed ...

A summary of current impressions on the treatment of leukemia by irradiation and bone marrow grafting is presented. Total-body irradiation alone produces remissions in some cases of acute leukemia. Remissions alone cannot be accepted as proof of survival of attempted marrow grafts. in most cases of acute leukemia the leukemic cells appear to be ...

Structural and cellular morphologic changes in rat bone marrow were correlated during the degenerative and regenerative phases following exposure to 550, 1000, and 1500 r of x radiation. Tritiated thymidine was injected 30 min prior to sacrifice. It was clearly demonstrated that the vascular bed of the marrow is destroyed, maximum damage at 3 ...

The neutrophilic granulocyte count in the blood responds quickly to infection in dogs. The ratio of non-segmented to segmented neutrophils increased progressively after induction of bacterial pneumonia showing a rapid release from the bone marrow of less mature cells. Under normal steady state conditions there are modest fluctuations in the blood granulocyte count, presumably ...

Four bergles were exposed to supralethal total-body irradiation from a 250 kw x-ray therapy machine. Four were exposed to similar irradiation from a cobalt-60 teletherapy unit. Dosages administsred ranged from 600 to 1500 r, calculatsd as air dose at theoretical atidbody line. After irradiation each dog was given an intravenous infusion of 1.4 to 3.7 billion cells of his own bone ...

We have previously reported that interleukin 1 (IL-1) administration 20 hr before irradiation protects mice from lethal effects of radiation. The recovery of total nucleated bone marrow cells and of hematopoietic progenitor cells was enhanced in IL-1 trea...

Rats were given whole-body x irradiation while the hind legs of the experimental animals were tied and immersed in ice water. The findings suggest combined hypothermia and ischemia of the hind legs during irradiation protected elements of the bone marrow which later contributed to th recovery of the animals from the radiation insult. (auth)

... Expression of adhesion molecules on CD34+ cells: CD34+ cells l-selectin+ cells predict a rapid platelet recovery after peripheral blood ... of microgravity on osteoblast growth and activation. Exp. Cell R...

... other factors that, in the presence of serious radiation injury of bone marrow, protect against death without stimulating ... A., M. Yonezawa, and N. Katoh. Restoration of radiation injury by ginseng. I. ...

This project involved two demonstration projects, one in a Marrow reservoir located in the southwestern part of the state and the second in the Cherokee Group in eastern Kansas. Morrow reservoirs of western Kansas are still actively being explored and con...

Dogs which ad been heavily irradiated one to two years previously were severely bled. Both control and recovered dogs responded to the bleeding in a similar manner, indicating complete functional recovery of the previously irradiated marrow. (auth)

A variety of glycoproteins with hormone-like stimulatory effects on hematopoietic progenitor cells have been purified and characterized. In recent articles, the molecular and biochemical properties of specific murine and human glycoprotein, granulocyte-ma...

with intramuscular injection of ket- amine/xylazine/buprenorphine and inhalation of 3% isoflur- ane/oxygen, each unrestricted ambu- lation in cages after recovery, and received buprenorphine analgesia twice at approximately

- amine/xylazine/buprenorphine and inhalation of 3% isoflur- ane/oxygen, each animal underwent sequential recovery, and received buprenorphine analgesia twice at approximately 1 h and 16 h following arthrotomy

Caregiving: Helping Your Loved One Through Transplant If someone you love is undergoing a bone marrow or cord blood transplant (also called a BMT), you ... you play an important role in your loved one's treatment and recovery. What to expect As a ...

Immature reticulocyte fraction a new routine parameter in the hematology analyzer can give the idea of the earliest morphologic change of bone marrow recovery before other test become positive after chemotherapy. A prospective observational study was carried out in the Department of Clinical Pathology in collaboration with Paediatric Haematology and ...

Acute, sublethal infection of mice with murine cytomegalovirus (MCMV) resulted in up to 80% decreases in the number of cells recoverable from the bone marrow, and a decrease in peripheral blood leucocyte counts during the first week of infection. Depopulation of the leucopoietic areas of the marrow was evident from examination of histological sections. The ...

To compare the ability of bone marrow mesenchymal stem cells (MSCs) and transdifferentiated Schwann-like MSCs (tMSCs) in promoting transected facial nerve branches repair in a rabbit model of injury, rabbit tMSCs were induced from bone marrow MSCs, and Schwann cells markers were assessed by Western blot analysis. The left facial nerve buccal branch was ...

Tissue-engineered nerve grafts (TENGs), typically consisting of a neural scaffold included with support cells and/or growth factors, represent a promising alternative to autologous nerve grafts for surgical repair of large peripheral nerve gaps. Here, we developed a new design of TENGs by introducing bone marrow mesenchymal stem cells (MSCs) of rats, as support cells, into a ...

Recombinant human Interleukin-1 Alpha (rhu IL-1 alpha) was assessed for its efficacy in modifying the immunosuppression of mice compromised by Cyclophosphamide (CY), retrovirus infection or surgical stress. Sublethal dose (300 mg/kg) of CY caused neutropenia, decreased cellularity of bone marrow and inhibited Natural Killer (NK) cell activity and lymphokine-activated killer ...

Our results demonstrate that IL 1 promotes hematopoiesis in normal and radiation-compromised animals. IL 1 protected mice from lethal hematopoietic syndrome when given before irradiation. Given therapeutically after irradiation, IL 1 promoted recovery from radiation injury. Several activities of IL 1 may explain its bone marrow restorative properties. The ...

OBJECTIVE: Whether a specific nutritional support promotes healing of aplastic anemia (AA) patients is still unclear. Therefore, we explored the potential of a high-nucleotide, arginine, and micronutrient nutritional supplement on the nutritional rehabilitation of AA mice. METHODS: The BALB/c AA mice model was treated with hypodermic injections of acetylphenylhydrazine (100 mg/kg), x-ray (2.0 Gy), ...

Aims The chemokine receptor CXCR4 modulates endothelial progenitor cell migration, homing, and differentiation, and plays a key role in cardiovascular regeneration. Here we examined the effect of ex vivo acidic preconditioning (AP) on CXCR4 expression and on the regenerative potential of mouse bone marrow (BM) ckit(+) cells. Methods and results Acidic preconditioning was ...