... Therapeutic Administrati in of Recombinant Human Granulocyte Colony- Stimulating Factor Accelerates Hemopoietic Regeneration and Enhances ...

The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate ...

Effect of the antiserotonin drug cyproheptadine on the erythropoiesis has been studied under conditions of the administration of cytostatics (fluoropyrimidine antimetabolite 5-fluorouracil (5-FU), alkylating agent cyclophosphane) with different mechanisms of action. It is established that the antiserotonin drug significantly accelerates regeneration of the ...

Intravenously injected bone marrow cells do not participate in the regeneration of hemopoietic stromal progenitors in irradiated mice, nor in the curetted parts of the recipient's marrow. The hemopoietic stromal progenitors in allogeneic chimeras are of recipient origin. The adherent cell layer (ACL) of long-term cultures of ...

The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate ...

C3H/HeN female mice were exposed to whole-body cobalt-60 radiation and administered soluble glucan (5 mg i.v. at 1 h following exposure), recombinant human granulocyte colony-stimulating factor (G-CSF; 2.5 micrograms/day s.c., days 3-12 following exposure), or both agents. Treatments were evaluated for their ability to enhance hemopoietic regeneration, and ...

Marrow cavities permanently aplastic after irradiation were mechanically disrupted and locally injected with bone marrow obtained from an opposite leg. Hemopoietic bone marrow regenerated with restoration of normal parenchymal and sinusoidal architecture....

Successful treatment of the hemopoietic phase of the acute irradiation syndrome in dogs at the LD/sub 100/ (400 r) dose is possible on an individual case basis with goed nursing care. The onset and rate of regeneration is related to the radiation dose. Partial regeneration with subsequent death occurs with a 550 r dose. The ...

... spleen colony-forming units) and enhanced ... hemopoietic regeneration after radiation-induced myelosuppression, 2 ... initiated 1 h following exposure. ...

When injected into a group of lethally irradiated syngeneic (inbred) Wistar Furth (WF) rats, suspensions of stromal cells grown in monolayer culture from the marrow of WF rats produced hemopoietic colonies in the spleen and rescued 50% of the rats, while 90% of the non-injected (control) rats died within 30 days and had no hemopoietic colonies in the ...

Partial deuteration of mice by ingestion of 29% heavy water for 12 days prior to irradiation lessened their susceptibility to lethal doses of whole body gamma irradiation from a /sup 60/Co source. Deuteration alone slightly reduced the number of nucleated bone marrow cells, blood leukocytes, and platelets. After exposure to 8.5 Gy, all mice drank tap water. Radiation-induced destruction of ...

Histopathological observation was carried out on the changes of the extramedullary hemopoietic tissue of spleen of young mice which had been irradiated with from 60 to 600 r of x rays. In addition to the observation, discussion was made on correlation between these changes and those in the bone marrow and on such correlation in connection with changes in the ...

Dogs which ad been heavily irradiated one to two years previously were severely bled. Both control and recovered dogs responded to the bleeding in a similar manner, indicating complete functional recovery of the previously irradiated marrow. (auth)

The regeneration of hemopoietic precursor cells was monitored in spleen organ cultures from lethally irradiated mice injected with 10(7) normal syngeneic or allogeneic bone marrow cells. The important role of the microenvironment in supporting hemopoiesis was confirmed by the failure of mutant Sl/Sld spleens to support CFC regeneration ...

It has generally been accepted that most biologically derived agents that are radioprotective in the hemopoietic-syndrome dose range (eg, endotoxin, Bacillus Calmette Guerin, Corynebacterium parvum, etc) exert their beneficial properties by enhancing hemopoietic recovery and hence, by regenerating the host's ability to resist ...

It has generally been accepted that most biologically derived agents that are radioprotective in the hemopoietic-syndrome dose range (eg, endotoxin, Bacillus Calmette Guerin, Corynebacterium parvum, etc) exert their beneficial properties by enhancing hemopoietic recovery and hence, by regenerating the host's ability to resist ...

Glucan, WR-2721, and selenium, three agents with distinct radioprotective mechanisms, were evaluated in C3H/HeN mice for survival-enhancing and hemopoietic-regenerating effects when administered alone or in combinations before exposure to 60Co radiation. At LD50/30 radiation doses (radiation doses lethal for 50% of mice within 30 days postexposure), dose reduction factors of ...

Hemopoietic aplasia is the primary limitation of drug and radiation cancer therapies. We have previously demonstrated that, individually, both interleukin-6 (IL-6) and granulocyte colony-stimulating factor (G-CSF) can accelerate recovery from radiation-in...

Ribbed newts were used for studying the effect of space flight on board of the biosatellite (Cosmos-2229) on blood and clonogenic hemopoietic cells. In blood of newts of the flight group, the relative proportion of neutrophils increased, whereas that of lymphocytes and eosinophils decreased. Space flight did not result in loss of the ability of newt blood cells to incorporate ...

C3H/HeN female mice were exposed to whole-body cobalt-60 radiation and administered soluble glucan (5 mg i.v. at 1 h following exposure), recombinant human granulocyte colony-stimulating factor or both agents. Treatments were evaluated for their ability to enhance hemopoietic regeneration, and to increase survival after radiation-induced myelosuppression. ...

After lethal irradiation of C57BL mice followed by the injection of 10/sup 7/ marrow cells, total cellularity and progenitor cell levels exceeded pretreatment levels within 12 days in the spleen, but regeneration remained incomplete in the marrow. The exceptional regenerative capacity of progenitor populations in the spleen was observed in organ cultures of spleen slices ...

... surface antigens. hemopoietic progenitor cell activity, and concentration stem cells (HSC). The hemopoietic progenitor ...

The regeneration of hemopoietic precursor cells (colony-forming cells, CFC) was monitored in spleen organ cultures from lethally irradiated mice injected with 10(7) normal syngeneic or allogeneic bone marrow cells. The important role of the microenvironment in supporting hemopoiesis was confirmed by the failure of mutant S1/S1d spleens to support CFC ...

These studies were performed to compare the effects of single and fractionated localized irradiation doses to bone marrow on depletion and recovery of various hemopoietic cell lines. Mice received radiation to right thighs while the rest of the body was shielded. Single exposures ranged from 1000 to 10,000 rad and fractionated exposures ranged from total doses of 1000 to 6000 ...

Effect of carbon treatment on retention of peripheral erythroches and on hemopoietic recovery following irradiation was studied. Life-span of /sup 51/Cr- labeled erythrocytes was shortened to about three-fourths that of normal mice by irradiation (500 R). By previously blockading RES with carbon particles, this accelerated destruction of ...

Dogs were given transfusions of cryopreserved autologous mononuclear blood leukocytes after 1200 roentgens (R) (midline dose) whole-body x-irradiation. Bone marrow repopulation was studied by means of histomorphological methods at days 9 and 10 after transfusion of an average of 3 x 10/sup 9/, 7 x 10/sup 9/, 13 x 10/sup 9/, and 31 x 10/sup 9/ cells. The return of marrow cellularity to normal ...

Hemopoietic stem cells were less sensitive to killing by a dose of radiation, if it were delivered in two fractions separated in time. The magnitude of this decrease in sensitivity was greater when the cells were irradiated in the normal steady state, compared with a regenerating state. However, the effect was reversed when donor mice received ...

... Title : The Use of Hemopoietic Marrow Transplants in Inducing Osteogenesis in the Surgical Repair of Osseous Defects. ...

... Title : CHEMICAL RADIOPROTECTION OF HEMOPOIETIC COLONY-FORMING CELLS: COMPARATIVE EFFECT OF AET, ANOXIA AND ...

We studied the ability of the hemopoietic organ stroma to recover from damage inflicted by 5 or 7 Gy gamma radiation administered during a period of stromal growth in 4-week-old mice. Irradiation resulted in an immediate depletion of femoral colony-forming fibroblastic progenitors (CFU-F) down to 10-20% of age-matched control values. A full recovery to normal numbers occurred ...

Two hundred male white rats, weighing 130 to 150 g, were injected with neutral red and /sup 90/Sr chloride, 0.1 mu C/g, which resulted in death after two years. Five animals were sacrificed each month, and stained marrow smears were studied. A definite cycle in the changes in the cells of the marrow was noted. In the first three months, physiological regeneration ...

The influence of IL-1 administration on the recovery of the hemopoietic and immune systems from sublethal irradiation was assessed. Mice were irradiated (750 R) and injected twice daily with purified recombinant derived IL-1 beta (200 ng/injection). At various times after irradiation, the functional capacity of the hemopoietic and immune systems was ...

... Title : Regenerative Flywheel Energy Storage System,. ... a portion of the vehicle's accelerating energy and regenerates the vehicle's braking energy. ...

Quantitative histological study revealed that regeneration of sectioned guinea pig facial nerve is accelerated by exposure to a moderate hypergravitic field of 1.6g. A two fold increase in rate of regeneration and in sprouting was observed. Scar tissue formation was significantly reduced in animals exposed to the hypergravitic field. ...

Mortality of mice irradiated several weeks after administration of mycobacterium tuberculosis and zymosan was significantly reduced. There was no accelerated recovery of hemopoietic and lymphatic tissue in the protected animals. Certain enhanced activities of the reticuloendothelial system related to increased nonspeciflc resistance were still ...

Cells from regenerating mouse liver removed 2 to 25 days post 68 percent hepatic resection have been assayed for in vitro colony forming capacity in soft agar (CFU-C), proliferative capacity in liquid culture, and in vivo spleen colony forming capacity (CFU-S). These studies demonstrated low concentrations of CFU-C and CFU-S in normal and sham-operated liver, with an ...

... aimed at investigating radiation-induced hemopoietic and ... were specific for pluripotent hemopoietic stem ... dogs to investigate stem cell survival in ...

Analysis was made of the mechanism of therapeutic application of thiamine diphosphate (TDP) in radiation sickness in mice. This agent increased the number of endogenous colonies in the spleen and incorporation of /sup 59/Fe in spleen and bone marrow with sublethal doses of radiation (500 and 600 R) and has no effect with a lethal dose (750 R). After administration of TDP to mice exposed to 500 R ...