SUMMARYIn dividing cells, nuclear pore complexes (NPCs) disassemble during mitosis and reassemble into the newly forming nuclei. However, the fate of these multi-protein transport channels in post-mitotic cells, where the mitotic renewal of pores is absent, is unknown. Here we show that NPCs, unlike other nuclear ...

One main feature of apoptosis is the sequential degradation of the nuclear structure, including the fragmentation of chromatin and caspase-mediated cleavage of various nuclear proteins. Among these proteins is the Nuclear Mitotic Apparatus protein (NuMA) which plays a specific role in the organization of the ...

... Descriptors : (*LINEAR ACCELERATORS, ELECTRON ACCELERATORS), (*ELECTRON ACCELERATORS, NUCLEAR SCATTERING), ELASTIC ...

The human checkpoint kinase Chk1 has been suggested as a target for cancer treatment. Here, we show that a new inhibitor of Chk1 kinase, CEP-3891, efficiently abrogates both the ionizing radiation (IR)-induced S and G(2) checkpoints. When the checkpoints were abrogated by CEP-3891, the majority (64%) of cells showed fragmented nuclei at 24 hours after IR (6 Gy). The formation of ...

... INDUCTION SYSTEMS, *ELECTRON ACCELERATORS, *PARTICLE ACCELERATORS, IMPEDANCE, BETATRONS, NUCLEAR STRUCTURE. ...

... nuclear cells were in the mitotic prophase of oogonia and the large nuclear cells were in the ... the differentiation of primordial germ cells (PGCs) to oogonia in females or to spermatogonia in males. Th...

All nuclear RNA synthesis is repressed during the mitotic phase of the cell cycle. In addition, RNA polymerase II (RNAP II), nascent RNA and many transcription factors disengage from DNA during mitosis. It has been proposed that mitotic transcription repression and disengagement of factors are due to either mitotic ...

Overexpression of the low molecular�weight isoforms (LMW-E) of cyclin E induces chromosome instability; however, the degree to which these tumor-specific forms cause genomic instability differs from that of full-length cyclin E (EL), and the underlying mechanism(s) have yet to be elucidated. Here, we show that EL and LMW-E overexpression impair the G2-M transition differently and lead to ...

... Accelerator Conference on Accelerator Science and Technology ... of the Nuclear and Plasma Sciences Society of ... with oral and poster presentations. ...

... Descriptors : *CYCLOTRON RESONANCE, *ION ACCELERATORS, DIODES ... Subject Categories : PARTICLE ACCELERATORS NUCLEAR ...

Human uterine fibroblasts are cultured continuously. Data are presented from studies on the effect of irradiation on cell growth and on the progress of the mitotic cycle. Results are reported from studies on the protective effects of a number of chemical agents on the biological effects of x rays on fibroblasts grown in tissue culture. Procedures are described for ...

... ACCELERATORS, NUCLEAR ENGINEERING, PARTICLE ACCELERATOR COMPONENTS ... CAPTURE, NUCLEAR RESONANCE, MAINTENANCE ...

... Accession Number : ADD331631. Title : Miniature Linear Accelerator for Radiography of Nuclear Plant Components,. Corporate Author : ...

... OF NUCLEAR REACTORS ON ELECTRON ... equations of reactor acceleration; solution of ... ELECTRIC POWER PRODUCTION, REACTOR KINETICS ...

14 Mitotic Spindle Motors J. M. Scholey and A. Mogilner 14.1 Microtubules, Motors and Mitosis products of each nuclear division, depends upon the action of the mitotic spindle, a protein machine chromatids (Karsenti and Vernos, 2001, Mitchison and Salmon, 2001, Wittman et al., 2001). Spindle morpho

Nuclear galectins participate in splicing of pre-mRNA. In this study we detected galectins-1, -2, -3 and -7 and their glycoligands in three types of cells: fibroblasts, cancer epithelial cells and melanoma cells. The results demonstrated that the nuclear expression of distinct types of galectins and their ligands in interphasic nuclei is dependent on the ...

Absolute reaction rate theory is applied to explain the increased time between successive synchronous mitotic nuclear divisions in the slime mold Physarum polycephalum when exposed to extremely low-frequency electromagnetic fields. By comparing the normal (control) mitotic cycle to the lengthened mitotic cycle of ...

Dose rate effects on the mechanisms of space radiation induced delayed genomic .... Task, Update of the Nuclear Fragmentation Cross Section Code NUCFRG2 ...

... CRYSTAL OSCILLATORS, *QUARTZ RESONATORS, FREQUENCY, LINEAR ACCELERATORS, MEASUREMENT, NUCLEAR REACTORS, ...

... SCIENTIFIC RESEARCH, PARTICLE ACCELERATOR TARGETS, NUCLEAR PARTICLES, NEUTRON ... TIN, NUCLEAR RESONANCE, ISOTOPES ...

... Descriptors : *EXPERIMENTAL DESIGN, *NUCLEAR PROPULSION, NUCLEAR EXPLOSIONS, RATIOS, COMPUTATIONS, ACCELERATION ...

Nuclear electric generator for accelerating charged propellant particles in electrostatic propulsion

... SHIELDING), (*NUCLEAR RADIATION, SHIELDING), LINEAR ... HAZARDS, MATERIALS, SAFETY ... NUCLEAR RADIATION SHIELD, PROTECTION & ...

The materials may be used on the International Space Station, ... such as nuclear accelerators, nuclear power plants, nuclear submarines and medical ...

... the dawn of the nuclear age, nuclear power ... the agency, charges it to 'accelerate and enlarge ... their territory (eg, uranium and plutonium, whether in ...

... The prlnoipal sources of spurious counts are nuclear interactions produced, in the ... small fraction of the geometrioal mean free path for nuclear ...

Particle Accelerators Tools of Nuclear Science "... but What is it Good for?" Energy from Nuclear Science Radiation in the Environment Appendix A Glossary of Nuclear Terms Appendix...

A successive pattern of nuclear divisions that result in mitotic waves has been observed within the coenocytic nonarticulated laticifers of embryos of Euphorbia marginata Pursh. These waves originate independently in the cotyledonary or hypocotyl portion of the laticifer and exhibit uni-or bidirectional movement at variable velocities. Individual nuclei or ...

Landfarming is a soil bioremediation technology practiced by oil refineries in order to reduce or eliminate hydrocarbons from petroleum sludge. The goal of the current study was to use Allium cepa bioassay to assess landfarming and landfarming with rice hulls amendment before and after hydrocarbons biodegradation assay in the laboratory. Three cytogenetic endpoints were used: ...

Ran GTPase is involved in several aspects of nuclear structure and function, including nucleocytoplasmic transport and nuclear envelope formation. Experiments using Xenopus egg extracts have shown that generation of Ran-GTP by the guanine nucleotide exchange factor RCC1 also plays roles in mitotic spindle assembly. Here, we have ...

Disassembly of nuclear pore complexes (NPCs) is a decisive event during mitotic entry in cells undergoing open mitosis, yet the molecular mechanisms underlying NPC disassembly are unknown. Using chemical inhibition and depletion experiments we show that NPC disassembly is a phosphorylation-driven process, dependent on CDK1 activity and supported by members ...

progenitor cells under treatment with imatinib in vitro. Leukemia, 19, 1483�1485. Bertrand, P., Lambert, SCentrosome aberrations after nilotinib and imatinib treatment in vitro are associated with mitotic myeloid leukaemia (CML) first line treatment, response rates of patients in accelerated or blastic phase

To understand how human tumor cells respond to the combined treatment with nocodazole and high LET radiation, alterations in cell cycle, mitotic disturbances and cell death were investigated in the present study. Human cervix carcinoma HeLa cells were exposed to nocodazole for 18 h immediately followed by high LET iron ion irradiation and displayed a sequence of events leading ...

The comparative study of the percentage nuclear area in brain tumors by the point-counting technique and digital analysis showed a good correlation between the results obtained. The investigation suggests the possibility of using the volume corrected mitotic index to tumors with uniformly distributed cells. PMID:1666306

Atommash Production Association's use of electron accelerators for defectoscopy of nuclear power station parts is described. The capabilities of LUE-15/15,000D and Linatron-2000 linear accelerators and B-18 betatron cyclical accelerator are described.

We describe the basic tenets of the current concepts of cancer biology, and review the recent advances on the suppressor role of senescence in tumor growth and the breakdown of this barrier during the origin of tumor growth. Senescence phenotype can be induced by (1) telomere attrition-induced senescence at the end of the cellular mitotic life span (MLS*) and (2) also by ...

An accelerator-driven subcritical nuclear system is briefly described that transmutes actinides and selected long-lived fission products. An application of this accelerator transmutation of nuclear waste (ATW) concept to spent fuel from a commercial nucle...

This report discusses the following topics in relation to the Bevalac accelerator: accelerator technology operations summary; nuclear science; biomedical research; and publications and presentations.

... of 26 months of operating, repairing, and maintaining the nuclear accelerator Superhilac at the Lawrence Berkeley Laboratory (LBL) are analyzed ...

The formation and maintenance of the bipolar mitotic spindle apparatus require a complex and balanced interplay of several mechanisms, including the stabilization and separation of polar microtubules and the action of various microtubule motors. Nonmicrotubule elements are also present throughout the spindle apparatus and have been proposed to provide a structural support for ...

Cells of strain RCP isolated from guinea pig kidney were irradiated at a rate of 150 r/min with 180-kv x rays to doses of 150, 300, and 450 r. Studies were made of variations of mitotic index and frequencies of nuclear damage after exposure. The earliest recognizable effect of irradiation was a transitory inhibition of mitotic ...

The nuclear envelope (NE) separates the cytoplasm and the cell nucleus of interphase eukaryotic cells and nuclear pore complexes (NPCs) mediate the macromolecular exchange between these two compartments. The NE and the NPCs of vertebrate cells disassemble during prophase and the nuclear pore proteins (nucleoporins) are distributed ...

LMNA mutations are responsible for a variety of genetic disorders, including muscular dystrophy, lipodystrophy, and certain progeroid syndromes, notably Hutchinson-Gilford Progeria. Although a number of clinical features of these disorders are suggestive of accelerated aging, it is not known whether cells derived from these patients exhibit cellular phenotypes associated with ...

LMNA mutations are responsible for a variety of genetic disorders, including muscular dystrophy, lipodystrophy, and certain progeroid syndromes, notably Hutchinson-Gilford Progeria. Although a number of clinical features of these disorders are suggestive of accelerated aging, it is not known whether cells derived from these patients exhibit cellular phenotypes associated with ...

Abstract Polo-like kinase 1 (Plk1) plays essential roles during multiple stages of mitosis by phosphorylating a number of substrates. Here we report that the atypical protein kinase Rio2 is a novel substrate of Plk1 and can be phosphorylated by Plk1 at S335, S380 and S548. Knockdown of Rio2 accelerates mitotic progression while overexpression of Rio2 ...

Condensation and segregation of mitotic chromosomes is a critical process for cellular propagation, and, in mammals, mitotic errors can contribute to the pathogenesis of cancer. In this report, we demonstrate that the retinoblastoma protein (pRB), a well-known regulator of progression through the G1 phase of the cell cycle, plays a critical role in ...

A method is described for increasing the intensity of accelerated light-nucleus beams in linear proton accelerators. To attain such increase, the fore-injector of the LU-20 linear proton accelerator, which is the injector of the synchrophasotron of the Jo...

A brief summary of the talks on accelerator physics presented at this conference, Intersections Between Particle and Nuclear Physics , is given. New accelerator facilities and technologies, beam cooling, and the experiments were part of these discussions.

I describe the future accelerator facilities that are currently foreseen for electroweak scale physics, neutrino physics, and nuclear structure. I will explore the physics justification for these machines, and suggest how the case for future accelerators can be made.

This talk gives a general but highly subjective overview of the expectation for accelerators and colliders for high energy physics, but not extended developments of accelerators and storage rings for application to nuclear structure physics, synchrotron r...

... Accession Number : ADA087626. Title : Options for Accelerating Economic Recovery after Nuclear Attack. Volume 3. Descriptive Note : Final rept. ...

... Accession Number : AD0057522. Title : Cosmic Rays, Nuclear Physics, and Linear Accelerator Studies. Descriptive Note : Annual rept. no. 4,. ...

... Accession Number : AD0024291. Title : Cosmic Rays, Nuclear Physics, and Linear Accelerator Studies. Descriptive Note : Annual rept. no. 3. ...

The discussion reviews the use of accelerators originally intended for nuclear physics to do high resolution mass spectrometry for the purpose of isotope dating and age estimation of materials. (GHT)

During open mitosis in higher eukaryotic cells, the nuclear envelope completely breaks down and then mitotic chromosomes are exposed in the cytoplasm. By contrast, mitosis in lower eukaryotes, including fungi, proceeds with the nucleus enclosed in an intact nuclear envelope. The mechanism of mitosis has been studied extensively in ...

Immediately following fertilization, the fruit fly embryo undergoes 13 rapid, synchronous, syncytial nuclear division cycles driven by maternal genes and proteins. During these mitotic cycles, there are barely detectable oscillations in the total level of B-type cyclins. In this paper, we propose a dynamical model for the molecular events underlying these ...

There is renewed interest in using accelerator driven neutron sources to address the problem of high-level long-lived nuclear waste. Several laboratories have developed systems that may have a significant impact on the future use of nuclear power, adding ...

Animal cloning by nuclear-transfer experi- ments frequently fails due to the inability of transplanted single mitosis is permitted. This mitotic remodel- ing involves a topoisomerase II-dependent shortening to produce cloned animals and to obtain new sources of mul- tipotential cells from differentiated tissues

BackgroundCellular terminal differentiation (TD) correlates with a permanent exit from the cell cycle and so TD cells become stably post-mitotic. However, TD cells express the molecular machinery necessary for cell proliferation that can be reactivated by experimental manipulation, yet it has not been reported the stable proliferation of any type of reactivated TD cells. ...

This report describes a bilateral seminoma in a stallion. After slaughter, histological examination revealed that the tumour consisted predominantly of polyhedral tumour cells with large nuclei, obvious nucleoli and a small border of cytoplasm. The mitotic index was low and Ki67 staining revealed 4% nuclear staining. To our knowledge, this paper is the ...

Trifluralin is a herbicide capable of interfering in mitotic cell division due to either microtubule depolymerization or alteration in the concentration of calcium ions within the cell. The aim of this study was to investigate the effects of trifluralin in Allium cepa meristematic cells, evaluating the induction mechanisms of the chromosomal and nuclear ...

Centrosome duplication is marked by discrete changes in centriole structure that occur in lockstep with cell cycle transitions. We show that mitotic regulators govern steps in centriole replication in Drosophila embryos. Cdc25^string, the expression of which initiates mitosis, is required for completion of daughter centriole assembly. ...

Bub3 is a conserved component of the mitotic spindle assembly complex. The protein is essential for early development in Bub3 gene-disrupted mice, evident from their failure to survive beyond day 6.5�7.5 postcoitus (pc). Bub3 null embryos appear normal up to day 3.5 pc but accumulate mitotic errors from days 4.5�6.5 pc in the form of micronuclei, ...

... Accession Number : AD0473825. Title : THE US ARMY NUCLEAR DEFENSE LABORATORY'S POSITIVE ION ACCELERATOR,. ...

... DOE produces both stable isotopes and radioisotopes. ... Radioisotopes are produced in nuclear reactors or particle accelerators. ...

... such as coral in the water, can accelerate the degradation of equipment. ... inspectors and border guards must use to combat nuclear smuggling. ...

Cell division in many metazoa is accompanied by the disassembly of the nuclear envelope and the assembly of the mitotic spindle. These dramatic structural rearrangements are reversed after mitosis, when the mitotic spindle is dismantled and the nuclear envelope reassembles. The targeting protein for XKlp2 (TPX2) ...

Gradients are used in a number of biological systems to transmit spatial information over a range of distances. The best studied are morphogen gradients where information is transmitted over many cell lengths. Smaller mitotic gradients reflect the need to organize several distinct events along the length of the mitotic spindle. The intracellular gradients ...

Skin fibroblast cells from two unrelated male infants with a chromosome-instability disorder were analyzed for their response to colcemid-induced mitotic-spindle checkpoint. The infants both had severe growth and developmental retardation, microcephaly, and Dandy-Walker anomaly; developed Wilms tumor; and one died at age 5 mo, the other at age 3 years. Their metaphases had ...

The effects of /sup 3/H-thymidine on cell progression through the mitotic cycle and on cell population kinetics both during and after exposure have been investigated. During exposure there is an accumulation of cells in G2 while the proportions of cells in G1, S and M decrease. Quiescent center cells are induced to synthesize DNA and presumably divide following inhibition of ...

for the specific mitotic functions of the Ran cycle in animal mitosis, the association of plant Ran plants is only just beginning, fundamental differences from the animal nuclear envelope have already been, and the role and fate of the nuclear envelope during mitosis. Key words: Lamin, NMCP1, nuclear envelope

... WEAPONS EFFECTS, *BLAST, *NUCLEAR EXPLOSION DAMAGE, ACCELERATION, ETIOLOGY, RESPONSE(BIOLOGY), GAS EMBOLISM ...

... Descriptors : *NUCLEAR RADIATION, *ELECTRON BEAMS, *LINEAR ACCELERATORS, TRANSISTORS, DAMAGE, SOLID STATE PHYSICS ...

... COMPOUNDS, *RARE GASES, *PROTON ACCELERATORS, EMISSION, QUENCHING, METASTABLE STATE, URANIUM COMPOUNDS. ...

... Title : HIGH-ENERGY PHYSICS. ... FIELD THEORY, PARTICLE ACCELERATORS, ELEMENTARY PARTICLES, NUCLEAR ...

... Descriptors : *NUCLEAR PHYSICS, *PARTICLES, ACCELERATION, BEVATRONS, COSMIC RAYS, DEGRADATION, ENERGY, THEORY. ...

... command and control, leadership and access ... accelerating investments in transformational capabilities. ... Nuclear Posture Review, which realigned ...