in the common goal of maximizing access to critical research. Improving the Lac System for Synthetic Biology Beta Beta Beta Biological Society Research Article Improving the Lac system for synthetic biology and catabolism. The lac promoter is the most commonly used promoter ...
E-print Network
Access to the article is free, however registration and sign-in are required. The lac repressor of Escherichia coli controls expression of enzymes involved in lactose metabolism by its interactions with inducer and with specific and nonspecific DNA. This has long been the model system for understanding transcriptional control. In his ...
NSDL National Science Digital Library
Different bacteria-derived systems for regulatable gene expression have been developed for the use in mammalian cells and some were also successfully adopted for in vivo use in vertebrate model organisms. However, certain limitations apply to most of these systems, including leakiness of transgene expression, inefficient transgene silencing or activation, ...
PubMed
PubMed Central
The gene encoding the lactose transport protein (lacS) of Leuconostoc lactis NZ6009 has been cloned from its native lactose plasmid, pNZ63, by functional complementation of lactose permease-deficient Escherichia coli mutants. Nucleotide sequence analysis revealed an open reading frame with the capacity to encode a protein of 639 amino acids which had limited but significant ...
... of fish, wildlife, and plants and their habitats. Legal and Policy Guidance The National Wildlife Refuge System ... items under their control o...
NBII National Biological Information Infrastructure
Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705alpha and Lac705beta. Neither Lac705alpha nor Lac705beta displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism ...
Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705? and Lac705?. Neither Lac705? nor Lac705? displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism of action, ...
The 5' region of the lac operon of Lactobacillus casei has been investigated. An open reading frame of 293 codons, designated lacT, was identified upstream of lacE. The gene product encoded by lacT is related to the family of transcriptional antiterminator proteins, which includes BglG from Escherichia coli, ArbG ...
... Accession Number : ADA269166. Title : Flood Control Project Lac Qui Parle, Emergency Plan. Descriptive Note : Final rept. for period ending Oct 88. ...
DTIC Science & Technology
This paper describes the monitoring electronics system design for the Liquid Argon Calorimeter (LAC) portion of the SLD detector. This system measures temperatures and liquid levels inside the LAC cryostat and transfers the results over a fiber-optic seri...
National Technical Information Service (NTIS)
The multicopy ?gt10-lacZ transgene shuttle vector of Muta�Mouse serves as an important tool for genotoxicity studies. Here, we describe a model for ?gt10-lacZ transgene molecular structure, based on characterisation of transgenes recovered from animals of our intramural breeding colony. Unique nucleotide sequences of the 47?513 bp monomer are reported ...
Tribal Cooperators Keweenaw Bay Indian Community Lac Vieux Desert Band of the Lake Superior Band of the Chippewa Indians Accessibility FOIA Privacy Policies and Notices Take...
Science.gov Websites
... Accession Number : AD0773707. Title : Light-Trap Catches of 'Culicoides' (Diptera: Ceratopogonidae) at Lac Serpent, Quebec,. ...
... Accession Number : AD0640612. Title : DIRECT EVIDENCE FOR AND TRANSITION RATES OF ION PAIR INTERACTIONS IN LAC13:HO3+,. ...
Chromatin immunoprecipitation (ChIP) has been used to detect binding of DNA-binding proteins to sites in nuclear and mitochondrial genomes. Here, we describe a method for detecting protein-binding sites on chloroplast DNA, using modifications to the nuclear ChIP procedures. The method was developed using the lac operator (lacO)/lac repressor ...
... des Lacs, Lac en Huit, Lac Intermédaire and Grand Lacs in southern New Caledonia. Occurs at 150 to ... ...
This report presents the results of a geological survey of the Lac du Bonnet Batholith in Manitoba. The survey consisted of field mapping of the lithologies and the joint systems throughout the batholith, and the examination of lineaments identified on ae...
The eclipsing RS CVn system AR Lac (G2IV + K0IV) has been observed continuously for one 2-day binary
NASA Technical Reports Server (NTRS)
The Escherichia coli LacI protein represses transcription of the lac operon by blocking access to the promoter through binding at a promoter-proximal DNA operator. The affinity of tetrameric LacI (and therefore the repression efficiency) is enhanced by simultaneous binding to an auxiliary operator, forming a DNA ...
NASA Astrophysics Data System (ADS)
Asia LA&C Oceania Europe N. America Global water supply 62% 81% 85% 88% 96% 100% Global sanitation 60) shows that in every region, except Oceania and Northern America, total sanitation coverage is lower than Africa Asia LA&C Oceania Europe N. America Water supply Household connection Other access No access ...
There is general recognition that engineering issues are critical to the viability of liquid argon calorimetry (LAC) at the Superconducting Super Collider (SSC). We have undertaken to quantitatively address these issues and, if possible, perform a preliminary design of a proof of principle'' LAC for SSC. To establish ...
Energy Citations Database
Organ morphogenesis requires the coordinated activity of many mechanisms involved in cell rearrangements, size control, cell proliferation and organ integrity. Here we report that Lachesin (Lac), a cell surface protein, is required for the proper morphogenesis of the Drosophila tracheal system. Homozygous embryos for Lac mutations, ...
