Determination of precise and accurate protein structures by NMR generally requires weeks or even months to acquire and interpret all the necessary NMR data.
Energy Citations Database
A method is described for the estimation of serum protein-bound iodine using alkaline incineration and an automated technique for the estimation of iodine in the ash. Pretreatment of the serum with an anion exchange resin avoids the need for precipitation and washing of the protein. The method is accurate, ...
PubMed Central
Despite significant advances in the identification of known proteins, the analysis of unknown proteins by MS/MS still remains a challenging open problem. Although Klaus Biemann recognized the potential of MS/MS for sequencing of unknown proteins in the 1980s, low throughput Edman degradation followed by cloning still remains the main ...
PubMed
Molecular modeling of proteins including homology modeling, structure determination, and knowledge-based protein design requires tools to evaluate and refine three-dimensional protein structures. Steric clash is one of the artifacts prevalent in low-resolution structures and homology models. Steric clashes arise due to the unnatural ...
The Pcons.net Meta Server (http://pcons.net) provides improved automated tools for protein structure prediction and analysis using consensus. It essentially implements all the steps necessary to produce a high quality model of a protein. The whole process is fully automated and a potential user only submits the ...
... Automated Pipeline for Genome-Wide Protein ... automated ontology mapping generation algorithm ... automatically generate mappings between terms ...
DTIC Science & Technology
... Title : BIOLOGICALLY INSPIRED SENSOR FUSION,. Corporate Author : ACCURATE AUTOMATION CORP CHATTANOOGA TN. ...
In the present work we report on a novel and fast protocol for accurate bottom-up protein quantification that overcomes the drawbacks of in-gel digestion and MALDI analysis, while maintaining their benefits. It relies on the following steps: (i) gel electrophoresis separation of proteins, (ii) fast in-gel protein ...
The use of sub-nanometer resolution electron density as spatial constraints for de novo and ab initio structure prediction requires knowledge of protein boundaries to accurately segment the electron density for the prediction algorithms. Here we present a procedure where even poorly segmented density can be used to determine the fold of the ...
identified by qRT�PCR for Hexokinase 2 (Hk2) and Docking protein 1 (Dok1) at four months and for Dok1 and Chr 10 QTL. HtrA serine peptidase 2 (Htra2), Dok1, and Tnfaip3 were deemed most promising because Tnfaip3 was excluded as the QTG for the Chr 10 QTL, Dok1 and Htra2 remain good candidates for the Chr 6
E-print Network
The Field of Automated Reasoning* M Larry Wos athematics and Computer Science Division Argonne National Laboratory Argonne, IL 60439�4801 T Abstract he term automated reasoning (first introduced in 1980) accurately describes the objective of the field, d the automation of logical reasoning. This article
It is now easier to discover thousands of protein sequences in a new microbial genome than it is to biochemically characterize the specific activity of a single protein of unknown function. The molecular functions of protein sequences have typically been predicted using homology-based computational methods, which rely on the principle ...
NASA Astrophysics Data System (ADS)
The scientific value of experimental protein crystal growth has been well established ... of the final Automated Protein Crystal Growth Facility design. ...
NASA Website
Despite recent efforts to develop automated protein structure determination protocols, structural genomics projects are slow in generating fold assignments for complete proteomes, and spatial structures remain unknown for many protein families. Alternative cheap and fast methods to assign folds using prediction algorithms continue to ...
... art for a highly accurate, automated, all-weather azimuth capability which ... use gimbal-mounted rate gyrocompasses to indicate the azimuths of two ...
Automated Digital Terrain Model Generation from HiRISE and LROC Stereo ... Martian surface by providing Geologists with a highly accurate elevation map of ...
between air traffic control and aircraft via data link. vFlight crews follow these trajectories accurately using the aircraft's FMS automation. ...
Thermal tolerance has been identified as an important factor relevant to the pathogenicity of Enterobacter sakazakii in human neonates. To identify a biomarker specific for this phenotypic trait, intact protein expression profiles of 12 strains of E. sakazakii were obtained using liquid chromatography mass spectrometry. Proteins were extracted from the ...
The creation of an automated method for determining 3D protein structure would be invaluable to the field of biology and presents an interesting challenge to computer science. Unfortunately, given the current level of protein knowledge, a completely autom...
National Technical Information Service (NTIS)
... of Global Earthquakes for Response) is an automated alarm system being developed to rapidly and accurately assess the ... ...
NBII National Biological Information Infrastructure
Accurate Automation Corporation proposes to design a system for reducing the friction drag on an aircraft by introducing an ion space charge into the ...
... Title : Biologically Inspired Sensor Fusion. Corporate Author : ACCURATE AUTOMATION CORP CHATTANOOGA TN. Personal ...
... Title : BIOLOGICALLY INSPIRED FUSION OF SONAR SENSOR DATA,. Corporate Author : ACCURATE AUTOMATION CORP CHATTANOOGA TN. ...
... Abstract- This study describes how to establish a capillary viscometer (microviscosimeter) that uses an inline micro- flowmeter that can accurately ...
This study presents a fully automated membrane segmentation technique for immunohistochemical tissue images with membrane staining, which is a critical task in computerized immunohistochemistry (IHC). Membrane segmentation is particularly tricky in immunohistochemical tissue images because the cellular membranes are visible only in the stained tracts of the cell, while the ...
Designing effective and accurate tools for identifying the functional and structural elements in a genome remains at the frontier of genome annotation owing to incompleteness and inaccuracy of the data, limitations in the computational models, and shifting paradigms in genomics, such as alternative splicing. We present a methodology for the automated ...
accurate, real-time information to support manufacturing, distribution, stock control and service tracking technologies in the development of improved product support services. The Cambridge Auto-ID LabIntelligent automation for the manufacturing supply chain Distributed Information and Automation
Quantitative proteomics relies on accurate protein identification, which often is carried out by automated searching of a sequence database with tandem mass spectra of peptides. When these spectra contain limited information, automated searches may lead to incorrect peptide identifications. It is therefore ...
The present invention relates to automated methods, systems, and apparatuses for protein separation and analysis. In particular, the present invention provides an automated system for the separation, identification, and characterization of the phosphoryla...
PTMSearchPlus is a software tool for the automated integration of accurate intact protein mass (AIPM) and bottom-up (BU) mass spectra searches/data in order to both confidently identify the intact proteins and to characterize their post-translational modifications (PTMs). The development of PTMSearchPlus was ...
Exchange between protein backbone amide hydrogen and water gives relevant information about solvent accessibility and protein secondary structure stability. NMR spectroscopy provides a convenient tool to study these dynamic processes with saturation transfer experiments. Processing of this type of NMR spectra has traditionally required peak integration ...
Thermal stability of proteins is crucial for both biotechnological and therapeutic applications. Rational protein engineering therefore frequently aims at increasing thermal stability by introducing stabilizing mutations. The accurate prediction of the thermodynamic consequences caused by mutations, however, is highly challenging as ...
AbstractThermal stability of proteins is crucial for both biotechnological and therapeutic applications. Rational protein engineering therefore frequently aims at increasing thermal stability by introducing stabilizing mutations. The accurate prediction of the thermodynamic consequences caused by mutations, however, is highly ...
G protein-coupled receptors (GPCRs) are historically the richest targets for drug discovery, accounting for nearly 60 percent of prescription drugs. The ligands and functions of only 200 out of possibly 1000 GPCRs are known. Screening methods that directly and accurately measure GPCR activation and inhibition are required to identify ligands for orphan ...
Recent technological improvements have made it possible to examine the dynamics of individual vesicles at a very high temporal and spatial resolution. Quantification of the dynamic properties of secretory vesicles is labor-intensive and therefore it is crucial to develop software to automate the process of analyzing vesicle dynamics. Dual-threshold and binary image conversion ...
BackgroundWhen accurate models for the divergent evolution of protein sequences are integrated with complementary biological information, such as folded protein structures, analyses of the combined data often lead to new hypotheses about molecular physiology. This represents an excellent example of how bioinformatics can be used to ...
Leucine-rich repeat (LRR) proteins form a large and diverse family. They have a wide range of functions most of which involve the formation of protein-protein interactions. All known LRR structures form curved solenoids, although there is large variation in their curvature. It is this curvature that determines the shape and dimensions of the inner space ...
Systematic benchmarking of multi-dimensional protein NMR experiments is a critical prerequisite for optimal allocation of NMR resources for structural analysis of challenging proteins, e.g. large proteins with limited solubility or proteins prone to aggregation. We propose a set of benchmarking parameters for ...
When analyzing protein sequences using sequence similarity searches, orthologous sequences (that diverged by speciation) are more reliable predictors of a new protein's function than paralogous sequences (that diverged by gene duplication) The utility of phylogenetic information in high-throughput genome annotation ("phylogenomics") is widely ...
The System Management and Production Laboratory at the Research Institute, the University of Alabama
NASA Technical Reports Server (NTRS)
Inference of protein functions is one of the most important aims of modern biology. To fully exploit the large volumes of genomic data typically produced in modern-day genomic experiments, automated computational methods for protein function prediction are urgently needed. Established methods use sequence or structure similarity to ...
Inference of protein functions is one of the most important aims of modern biology. To fully exploit the large volumes of genomic data typically produced in modern-day genomic experiments, automated computational methods for protein function prediction are urgently needed. ...
Crystals of many important biological macromolecules diffract to limited resolution, rendering accurate model building and refinement difficult and time-consuming. We present a torsional optimization protocol that is applicable to many such situations and combines Protein Data Bank-based torsional optimization with real-space refinement against the ...
A centrifuge-based microfluidic system has been developed that enables automated high-throughput and low-volume protein crystallizations. In this system, protein solution was automatically and accurately metered and dispensed into nanoliter-sized multiple reaction chambers, and it was mixed with various types of ...
We present sequence alignment software, called PTMap, for the accurate identification of full-spectrum protein post-translational modifications (PTMs) and polymorphisms. The software incorporates several features to improve searching speed and accuracy, including peak selection, adjustment of inaccurate mass shifts, and precise localization of PTM sites. ...
Protein-protein interaction (PPI) is at the core of the entire interactomic system of any living organism. Although there are many human protein-protein interaction links being experimentally determined, the number is still relatively very few compared to the estimation that there are ?300,000 protein-protein interactions in human ...
Novel high-throughput sample preparation strategies for MALDI imaging mass spectrometry (IMS) and profiling are presented. An acoustic reagent multispotter was developed to provide improved reproducibility for depositing matrix onto a sample surface, for example, such as a tissue section. The unique design of the acoustic droplet ejector and its optimization for depositing matrix solution are ...
BackgroundThe ability of mature forms of Plasmodium falciparum infected erythrocytes to bind to a range of host receptors including those displayed on endothelial cells has been associated with the pathology of this infection. Investigations into this adhesive phenomenon have used protein and cell-based adhesion assays to quantify the ability of infected red blood cells to ...
An automated procedure for the simultaneous determination of serum total protein and globulin is presented. The total protein is determined by the biuret reaction while the serum globulin is determined by the reaction of globulins with copper in the prese...
The increasing size of protein sequence databases is straining methods of sequence analysis, even as the increased information offers opportunities for sophisticated analyses of protein structure, function and evolution. Here we describe a method called Meta-MEME that uses artificial intelligence-based algorithms to build models of families of ...
Microbial engineering often requires fine control over protein expression; for example, to connect genetic circuits 1-7 or control flux through a metabolic pathway 8-13. We have developed a predictive design method for synthetic ribosome binding sites that enables the rational control of a protein's production rate on a proportional scale. Experimental ...
While Eukarya, Bacteria, and Archaea are all capable of protein N glycosylation, the archaeal version of this posttranslational modification is the least understood. To redress this imbalance, recent studies of the halophilic archaeon Haloferax volcanii have identified a gene cluster encoding the Agl proteins involved in the assembly and attachment of a ...
Determination of high-quality small protein structures by nuclear magnetic resonance (NMR) methods generally requires acquisition and analysis of an extensive set of structural constraints. The process generally demands extensive backbone and sidechain resonance assignments, and weeks or even months of data collection and interpretation. Here we demonstrate rapid and ...
Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified ...
A program was initiated at the Air Force Geophysics Laboratory to develop an automated system to provide accurate and timely measurements of temperature/dew point, winds, precipitation, pressure, visibility, and cloud height in a tactical bare-base airfie...
This paper suggests an improved measure for evaluating the usefulness of automated machine language translators. With the Global War on Terror (GWOT), the Army has increasing interest and need for accurate language translation more than ever. Today, there...
Aviation Applications of Automated Aircraft Weather Data Examples from meteorologists in forecast offices Richard Mamrosh National Weather Service Green Bay, Wisconsin #12;Difficulties in Aviation Forecasting Accurate aviation forecasts require a frequent and dense network of both surface and upper air
In this study, an automated formation control system for an aircraft formation comprised of one lead and multiple wing aircraft is analyzed. Second-order models of the C-130 aircraft are developed in order to accurately model the flying qualities of large...
A mathematical method of indexing planar deformation features in quartz and a Microsoft Excel macro for automated indexing is presented, allowing for more rapid and accurate results than the previously used manual method.
Markov state models (MSMs) are a powerful tool for modeling both the thermodynamics and kinetics of molecular systems. In addition, they provide a rigorous means to combine information from multiple sources into a single model and to direct future simulations?experiments to minimize uncertainties in the model. However, constructing MSMs is challenging because doing so requires decomposing the ...
Markov state models (MSMs) are a powerful tool for modeling both the thermodynamics and kinetics of molecular systems. In addition, they provide a rigorous means to combine information from multiple sources into a single model and to direct future simulations/experiments to minimize uncertainties in the model. However, constructing MSMs is challenging because doing so requires decomposing the ...
A procedure for determining the absolute activity of /sup 14/C-labeled and /sup 3/H-labeled solutes in a mixture from the measured counts per minute in two scintillation energy windows is described. It is shown that the method described here provides a substantially more accurate determination of /sup 3/H activity in the presence of a larger /sup 14/C activity, and a more ...
Automated analysis of gel images is an important part of the quantitative study of proteins that have been separated by two-dimensional gel electrophoresis. In order to establish the sensitivity of assays relying on this separation technique it is crucial to know how accurately and reliably information can be extracted from gel images. ...
The invention is a jig fixture for locating and supporting metal castings in an accurate manner to enable cleaning or machining operations to be performed in an automated manner on the castings.
Accurate Automation has developed a flush air data sensor comprised of a pressure- ... This system can be used on all military and commercial aircraft. ...
The fundamental goal of this project was to develop additional capabilities to the diamond measuring prototype, work out technical difficulties associated with the original device, and perform automated measurements which are accurate and repeatable. For ...
... We have developed an automated (using LabView) test set-up to accurately measure the frequency offset between two clocks, and used it to ...
goal being the development of the most robust and accurate refractometer .... detector slit was now in the focal plane of our refractometer camera mirror. ...
Jun 16, 2011 ... Accurate control of the press is obtained through an electrical servo activated press pump and pressure- and position-monitoring transducers. ...
Treesearch
... The deflection medium is a crystal material TeO2. The power of the electrical signal is 2W. ... FRadio-frequency Power Amplifier ...
This small 3D scanning device, called the Mold Impression Laser Tool or MILT, will offer an automated, accurate, systematic, and reliable means of assessing ...
