Current algorithms for quantifying peptide identification confidence in the accurate mass and time (AMT) tag approach assume that the AMT tags themselves have been correctly identified. However, there is uncertainty in the identification of AMT tags, because this is based on matching LC-MS/MS ...

MALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and ...

MALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and ...

We describe the application of capillary liquid chromatography (LC) time-of-flight (TOF) mass spectrometric instrumentation for the rapid characterization of microbial proteomes. Previously (Lipton et al. Proc. Natl Acad. Sci. USA, 99, 2002, 11049) the peptides from a series of growth conditions of Deinococcus radiodurans have been characterized using capillary LC MS/MS and ...

A review is given of nonleptonic decays of D mesons. Measurements of the psi (3772), the accurate determination of D masses and their consequences, inclusive measurements and tagged events are given. 36 references. (ERA citation 03:034070)

of the Deinococcus radio- durans proteome by using accurate mass tags. Proc. Natl Acad. Sci. USA 99, 11049�11054 21

Biofuels from photosynthetic microalgae are quickly gaining interest as a viable carbon-neutral energy source. Typically, characterization of algal feedstock involves breaking down triacylglycerols (TAG) and other intact lipids, followed by derivatization of the fatty acids to fatty acid methyl esters prior to analysis by gas chromatography (GC). However, knowledge of the ...

High-throughput proteomics is rapidly evolving to require high mass measurement accuracy for a variety of different applications. Increased mass measurement accuracy in bottom-up proteomics specifically allows for an improved ability to distinguish and characterize detected MS features, which may in turn be identified by, e.g., matching to entries in a ...

Isotope-labeled N-acetyl dipeptides (Ac-Xxx-Ala) are coupled to the primary amines of tryptic peptides and then analyzed by tandem mass spectrometry. Amide bond cleavage between Xxx and Ala provides both low- and high-mass isotope-coded signals for quantification of peptides. Especially, facile cleavage at the modified lysine side chain yields very strong ...

... tagging, recovery data, alternative tagging approaches and concomitant benefits/risks, and the demonstration tagging of hatchery stock at ... ...

Preparation of this manual was motivated by a need for a comprehensive field guide covering the application of internal wire tags in the mass tagging of sea turtle hatchlings and the detection of those tags later in the turtle's life. This manual is based...

Understanding biological systems and the roles of their constituents is facilitated by the ability to make quantitative, sensitive, and comprehensive measurements of how their proteome changes, e.g., in response to environmental perturbations. To this end, we have developed a high-throughput methodology to characterize an organism's dynamic proteome based on the combination of global enzymatic ...

The ability to understand biological systems and their constituents would be greatly facilitated by the ability to make quantitative, sensitive, and comprehensive measurements of how their proteome changes e.g. in response to environmental perturbations. To this end we have developed new instrumentation and a high throughput methodology to characterize an organism's dynamic proteome based ...

In mammalian cells, when tandem affinity purification approach is employed, the existence of untagged endogenous target protein and repetitive washing steps together result in overall low yield of purified/stable complexes and the loss of weakly and transiently interacting partners of biological significance. To avoid the trade-offs involving in methodological sensitivity, precision, and ...

Progress is reviewed towards the development of a global strategy that aims to extend the sensitivity, dynamic range, comprehensiveness and throughput of proteomic measurements based upon the use of high performance separations and mass spectrometry. The approach uses high accuracy mass measurements from Fourier transform ion cyclotron resonance ...

Despite many efforts in the last decade, the progress in de novo peptide sequencing has been slow with only 30�45% of all peptides correctly reconstructed. We argue that accurate full-length peptide sequencing may be an unattainable goal for some spectra and demonstrate how to accurately sequence gapped peptides instead. We further argue that gapped ...

Triacylglycerols (TAG) of lard, tallow, egg yolk, chicken skin, palm oil, palm olein, palm stearin, and a transesterified blend of palm stearin and coconut oil (82:18) were investigated by chemical ionization and collision-induced dissociation tandem mass spectrometry. Accurate molecular level information of the regioisomeric ...

The concept of using mass releases of juvenile radio tags represents a new and potentially powerful research tool that could be effectively applied to juvenile salmonid passage problems at dams on the Columbia and Snake Rivers. A system of detector antennas, strategically located, would automatically detect and record individually ...

Hybrid FTMS instruments, such as the LTQ-FTTM and LTQ-OrbitrapTM, are capable of generating fast duty cycle linear ion trap MS/MS data along with high resolution information without compromising the overall throughput of measurements. Combined with online LC separations, these instruments represent powerful and flexible tools for proteomics research. In the present work, we explore strategies for ...

Proteomics, and the larger field of systems biology, have recently demonstrated utility in both the understanding of cellular processes on the molecular level and the identification of potential biomarkers of various disease states. The large amount of data generated by utilizing high mass accuracy mass spectrometry for high-throughput proteomics analyses ...

Quantitative analysis of liquid chromatography (LC)- mass spectrometry (MS) and tandem mass spectrometry (MS/MS) data is essential to many proteomics studies. We have developed MASIC to accurately measure peptide abundances and LC elution times in low-resolution LC-MS/MS analyses. This software program uses an efficient processing ...

Identification of novel diagnostic or therapeutic biomarkers from human blood plasma would benefit significantly from quantitative measurements of the proteome constituents over a range of physiological conditions. We describe here an initial demonstration of proteome-wide quantitative analysis of human plasma. The approach utilizes post-digestion trypsin-catalyzed 16O/18O labeling, ...

A method for typing single nucleotide polymorphisms (SNPs) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described, in which a mass-tagged dideoxynucleoside triphosphate is employed in a primer extension reaction in place of an unmodified dideoxynucleoside triphosphate (ddNTP). The increased ...

Characterization of the human blood plasma proteome is critical to the discovery of routinely useful clinical biomarkers. We used an Accurate Mass and Time (AMT) tag strategy with high-resolution mass accuracy capillary liquid chromatography Fourier-Transform Ion Cyclotron Resonance Mass ...

Dec 6, 2004 ... RFID sensor tag affixed to each container to send accurate ... ChemSecure places RFID tags on hazardous material containers and uses Oracle ...

We describe and demonstrate a global strategy that extends the sensitivity, dynamic range, comprehensiveness, and throughput of proteomic measurements based upon the use of peptide accurate mass tags (AMTs) produced by global protein enzymatic digestion. The two-stage strategy exploits Fourier transform-ion cyclotron resonance (FT-ICR) ...

We describe and demonstrate a global strategy that extends the sensitivity, dynamic range, comprehensiveness and throughput of proteomic measurements based upon the use of polypeptide''accurate mass tags'' (AMTs) produced by a global protein enzymatic digestion. The two stage strategy exploits ...

... eggs (recruitment) or the movement of adults (spillover). Sonic tagging technology offers the potential to accurately measure ... the testing of coded tags with the VR2 sonic receivers, has been conducted...

Peptide identification by tandem mass spectrometry (MS/MS) is one of the most important problems in proteomics. Recent advances in high throughput MS/MS experiments result in huge amount of spectra, and the peptide identification process should keep pace. In this paper, we strive to achieve high accuracy and efficiency for peptide identification with the presence of noise by a ...

Peptide identification by tandem mass spectrometry (MS/MS) is one of the most important problems in proteomics. Recent advances in high throughput MS/MS experiments result in huge amount of spectra, and the peptide identification process should keep pace. In this paper, we strive to achieve high accuracy and efficiency for peptide identification with the presence of noise by a ...

We have developed a method for protein identification with peptide mass fingerprinting and sequence tagging using nano liquid chromatography (LC)/Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). To achieve greater sensitivity, a nanoelectrospray (nano-ES) needle packed with reversed-phase medium was used and ...

Ceramides play a crucial role in divergent signaling events, including differentiation, senescence, proliferation, and apoptosis. Ceramides are a minor lipid component in terms of content; thus, highly sensitive detection is required for accurate quantification. The recently developed isobaric tags for relative and absolute quantitation (iTRAQ) method ...

With performance above 97 % accuracy for newspaper text, part of speech (POS) tagging might be considered a solved problem. Previous studies have shown that allowing the parser to resolve POS tag ambiguity does not improve performance. However, for grammar formalisms which use more fine-grained grammatical categories, for example TAG ...

Object is measured for mass and center of mass with accuracies of 0.01% and 0.14 respectively, using

High throughput liquid chromatograph-mass spectrometry (LC-MS) based proteomics analyses have necessitated development of software to manipulate large volumes of detailed data and produce confident peptide/protein identifications. VIPER unites important data processing steps in a single software package that can be used to visualize peptide mass and LC ...

Proteomics has recently demonstrated utility in understanding cellular processes on the molecular level as a component of systems biology approaches and for identifying potential biomarkers of various disease states. The large amount of data generated by utilizing high efficiency (e.g., chromatographic) separations coupled to high mass accuracy mass ...

Isobaric stable isotope tagging reagents such as tandem mass tags or isobaric tags for relative and absolute quantification enable multiplexed quantification of peptides via reporter ion signals in the low mass range of tandem mass spectra. Until recently, the poor recovery ...

The throughput for proteomics measurements that provide broad protein coverage is limited by the quality and speed of both the separations and the subsequent mass analysis; present analysis times can range anywhere from hours to days (or longer). We have explored the basis for ultrahigh-throughput proteomics measurements using high-speed reversed-phase liquid chromatography ...

The Pulsed Photon Activation (PPA) technique for single-phase water flow and two phase air/water flow is analyzed for a 2.69-cm I.D. horizontal pipe. PPA measurements were performed at the RPI Gaerttner LINAC Laboratory using the /sup 16/O(..gamma..,n)/sup 15/O reaction, and was compared to the Pulsed Neutron Activation (PNA) technique in signal strength both analytically and experimentally. The ...

BackgroundHigh-resolution tandem mass spectra can now be readily acquired with hybrid instruments, such as LTQ-Orbitrap and LTQ-FT, in high-throughput shotgun proteomics workflows. The improved spectral quality enables more accurate de novo sequencing for identification of post-translational modifications and amino acid polymorphisms.ResultsIn this study, ...

Simulation of Electron and Ion Lenses for Mass Spectrometry ... accurate performance simulations of mass spectrometers used for detection and measurement of ...

Tags are non-invasive features induced in the heart muscle that enable the tracking of heart motion. Each tag line, in fact, corresponds to a 3D tag surface that deforms with the heart muscle during the cardiac cycle. Tracking of tag surfaces deformation is useful for the analysis of left ventricular motion. ...

Microtags are microscopic computer-generated holograms with 130-nm features and are mass- producible with EUVL. This fabrication method renders microtags difficult to counterfeit. Applications include tagging and tracking of microprocessors, memory chips, currency, and credit cards.

Microtags are microscopic computer-generated holograms with 130-nm features and are mass-producible with EUVL. This fabrication method renders microtags difficult to counterfeit. Applications includ tagging and tracking of microprocessors, memory chips, currencey, and credit cards.

ObjectivesTo characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach.Design and methodsLipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000�2005) and 10 ...

Objectives: To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach. Design and methods: Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and ...

Primary and secondary amines, including amino acids, biogenic amines, hormones, neurotransmitters, and plant siderophores, are readily derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using easily performed experimental methodology. Complex mixtures of these amine derivatives can be fractionated and quantified using liquid chromatography-electrospray ...

Proteomes from aerobic and photosynthetic grown Rhodobacter sphaeroides 2.4.1 cell cultures were characterized using liquid chromatography-mass spectrometry in conjunction with an accurate mass and elution time (AMT) tag approach. Roughly 8000 high quality peptides were detected that represented 1,445 gene products ...

The advent of high performance gradients and parallel imaging schemes has made real-time MRI a stable mode of scanning. Cardiac tagging techniques have been shown to give extremely precise and accurate estimates of the evaluation of myocardial function, W...

It took up to 10 million years after the mass extinctions for the ecosystem to stabilize. The study correlates ammonoid diversity and disparity and ...

... Descriptors : *HEMORRHAGIC FEVERS, *VIRUSES, REPRINTS, HIGH RATE, POLYMERIZATION, CHAIN REACTIONS, DEATH, CLINICAL ...

... To identify the tagged protein, the immunoprecipitates were digested with trypsin into peptides for analysis with liquid chromatography-mass ...

...denaturants may be made by volume, weight, accurate mass flow meter, or by any...spirits to be denatured by volume, weight, accurate mass flow meter, or other...denaturants to be used by volume, weight, accurate mass flow meter, or...

We describe an integrated suite of algorithms and software for general accurate mass and time (AMT) tagging data analysis of mass spectrometry data. The AMT approach combines identifications from liquid chromatography (LC) tandem mass spectrometry (MS/MS) data with peptide ...

Abstract The high-throughput accurate mass and time tag (AMT) proteomic approach was utilized to characterize the proteomes for cytoplasm, cytoplasmic membrane, periplasm, and outer membrane fractions from aerobic and photosynthetic cultures of the gram-nagtive bacterium Rhodobacter sphaeroides 2.4.1. In addition, we analyzed the ...

For use in the manufacture of gas tags such as employed in a nuclear reactor gas tagging failure detection system, a method for designing gas tagging compositions utilizes an analytical approach wherein the final composition of a first canister of tag gas as measured by a mass spectrometer is ...

For use in the manufacture of gas tags such as employed in a nuclear reactor gas tagging failure detection system, a method for designing gas tagging compositions utilizes an analytical approach wherein the final composition of a first canister of tag gas as measured by a mass spectrometer is ...

Oct 2, 2008 ... The GEOS-5 GCM run.script provides some functionality to archive the model output (restarts and history collections) to mass storage. ...

Generating all plausible de novo interpretations of a peptide tandem mass (MS/MS) spectrum (Spectral Dictionary) and quickly matching them against the database represent a recently emerged alternative approach to peptide identification. However, the sizes of the Spectral Dictionaries quickly grow with the peptide length making their generation impractical for long peptides. We ...

... design power limit was exceeded during these trials because the accurate setting of the thyristor control ... Each table contains the true wind speed ...

RFID tag standards moving is no longer accurate. Customer Support amp the 20th century it receiver is located in now the home of. Approximately 5 000 fire ...

Accurate centre-of-mass corrections for geodetic spherical satellites are expected to contribute to accurate determination of the scale of the earth, i.e. , ...

Bifilar pendulum, if great care is taken to provide accurate measurements, is very accurate (in .... Seismic accelerometers were able to measure mass line ...

With slight modifications of the instrumental parameters, we demonstrate that satisfactory top-down data can be obtained with collision-induced dissociation (CID) tandem mass spectrometry on a quadrupole time-of-flight (qTOF) instrument not originally designed for this purpose. Protein identification is achieved with both N- and C-terminal sequence tags ...

We describe the application of liquid chromatography coupled to mass spectrometry (LC/MS) without the use of stable isotope labeling for differential quantitative proteomics analysis of whole cell lysates of Shewanella oneidensis MR-1 cultured under aerobic and sub-oxic conditions. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was ...

Bronchoalveolar lavage fluid (BALF) contains proteins derived from various pulmonary cell types, secretions and blood. As the characterization of the BALF proteome will be instrumental in establishing potential biomarkers of pathophysiology in the lungs, the objective of this study was to contribute to the comprehensive collection of Mus musculus BALF proteins using high resolution and highly ...

We present a new development of the Tag-Mass concept based on a photocleavable linker with tagged molecules for polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) quantification coupled to mass spectrometry. PCR-MS and immunosorbent assay-MS with tagged oligonucleotides, bases, and ...

The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. ...

Obtaining accurate protein profiles from homogeneous cell populations in heterogeneous tissues can enhance the capability to discover protein biomarkers. In this context, methodologies to access specific cellular populations and analyze their proteome with exquisite sensitivity have to be selected. We report here the results of an investigation using a combination of laser ...

A FORTRAN 4 program is described which reduces the data obtained from a high resolution mass spectrograph. The program (1) calculates an accurate mass for each line on the photoplate, and (2) assigns elemental compositions to each accurate mass. The progr...

Liquid chromatography coupled to mass spectrometry (LC-MS) and tandem mass spectrometry (LC-MS/MS) has become a standard technique for analyzing complex peptide mixtures to determine composition and relative quantity. Several high-throughput proteomics techniques attempt to combine complementary results from multiple LC-MS and LC-MS/MS analyses to provide ...

The ALICE experiment is shown to be well suited for studies of exclusive final states from central diffractive reactions. The gluon-rich environment of the central system allows detailed QCD studies and searches for exotic meson states, such as glueballs, hybrids and new charmonium-like states. It would also provide a good testing ground for detailed studies of heavy quarkonia. Due to its central ...

Besides protein identification via mass spectrometric methods, protein and peptide quantification has become more and more important in order to tackle biological questions. Methods like differential gel electrophoresis or enzyme-linked immunosorbent assays have been used to assess protein concentrations, while stable isotope labeling methods are also well established in ...

... Accession Number : ADA187481. Title : Mass Spectral Investigations on Toxins. 7. Detection and Accurate Quantitation ...