The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available. Real-time PCR assays can be completed ...
PubMed
Rapid and accurate tests capable of distinguishing Bordetella pertussis from milder Bordetella parapertussis infection can aid in treating patients. We evaluated a novel real-time polymerase chain reaction (PCR) method that allows rapid and accurate diagnosis and distinction between B. pertussis and B. ...
There is a need to develop an accurate and rapid method to detect Spiroplasma citri, the causal agent of citrus stubborn disease for use in epidemiology studies. Quantitative real-time PCR was developed for detection of S. citri. Two sets of primers based on sequences from the P58 putative adhesin ...
Technology Transfer Automated Retrieval System (TEKTRAN)
Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical ...
... Accession Number : ADA449895. Title : Real-time PCR and PCR-tandem Mass Spectrometry for Biodetection. Descriptive Note : Briefing charts. ...
DTIC Science & Technology
... env�o y el almacenamiento a temperaturas no en congelaci�n, que puede ser importante para las pruebas de ... ...
NBII National Biological Information Infrastructure
... 19 noninfluenza respiratory and enteric pathogens, the analytical specificity of the M-gene assay was shown to be 100%. High diagnostic specificity of the assays was also confirmed by testing ... ...
A rapid and accurate minor groove binder (MGB) real-time PCR is described for detection lamivudine resistance mutations in hepatitis B virus. The real-time PCR was compared with direct Sanger sequencing in 53 clinical patients samples, the results of the real-time PCR correlate with the ...
The application of a real-time quantitative PCR method (5? nuclease assay), based on the use of a probe labeled at its 5? end with a stable, fluorescent lanthanide chelate, for the quantification of human fecal bifidobacteria was evaluated. The specificities of the primers and the primer-probe combination were evaluated by conventional PCR and real-time ...
PubMed Central
The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum ?-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum ...
OBJECTIVE: To develop a rapid, sensitive and specific assay method, based on multiplex real time PCR for identifying Vibrio cholerae and distinguishing Vibrio cholerae O139 serotype from Vibrio cholerae. METHODS: Cholera toxin A subunit gene (ctxA) and glycosyltransferase gene (LPSgt) were chosen as targets according to Vibrio cholerae ...
Accurate malaria diagnosis has dual roles in identification of symptomatic persons for effective malaria treatment and also numeration of asymptomatic persons who contribute to the epidemiologic determinants of transmission. Three currently used diagnostic tests: microscopy, rapid diagnostic tests (RDT) and real time ...
Abstract: Real-time PCR assays were used to measure the potential of the microbial population to degrade alkanes - the principal component of Special ...
NASA Website
... The SYBR Green I assay then followed by a ... given specimen based on the TaqMan technology ... This new real-time PCR allows the amplification and ...
... Accession Number : ADA429738. Title : Real-Time PCR Assay for a Unique Chromosomal Sequence of Bacillus anthracis. ...
... polymerase chain reaction (PCR) assays were developed ... real-time PCR assay, which used ... This sensitive and quantitative assay determination of ...
Culture-based Salmonella detection takes at least 4 d to complete. The use of TaqMan probes allows the real-time PCR technique to be a rapid and sensitive way to detect foodborne pathogens. However, unlike RNA-based PCR, DNA-based PCR techniques cannot differentiate between DNA from live and dead cells. Ethidium bromide monoazide (EMA) ...
We present a simple strategy for isolating and accurately enumerating target DNA from high-clay-content soils: desorption with buffers, an optional magnetic capture hybridization step, and quantitation via real-time PCR. With the developed technique, ?g quantities of DNA were extracted from mg samples of pure kaolinite and a field clay ...
Gene expression patterns are important determinants of a cell's state, and changes in the expression profile indicate adaptation processes as a response to developmental transitions or environmental changes. Assaying gene expression can, therefore, help to elucidate mechanisms of determination and differentiation, as well as signaling networks. Several methods have been employed to determine ...
Real-time reverse transcription polymerase chain reaction (RT-PCR) represents a benchmark technology in the detection and quantification of mRNA. Yet, accurate results cannot be realized without proper statistical analysis of RT-PCR data. Here, we examine some of the issues concerning RT-PCR ...
Timely response to the threat of the wheat stem rust (Puccinia graminis f.sp. tritici; Pgt) race TTKSK and its variants (the 'Ug99' lineage) requires the availability of sensitive, fast and easily implemented diagnostic assays to accurately monitor movement of the pathogen by pathologists, breeders ...
Real-time RT-PCR is the most sensitive and accurate method for mRNA quantification. Using specific recombinant DNA as a template, real-time PCR allows accurate quantification within a 7-log range and increased sensitivity below 10 copies. However, when using ...
Energy Citations Database
Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. PCR has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine ...
The operation of an electrochemical real-time PCR system, based on intercalative binding of methylene blue (MB) with dsDNA, has been demonstrated. PCR was performed on a fabricated electrode-patterned glass chip containing MB while recording the cathodic current peak by measuring the square wave voltammogram (SWV). The current peak ...
