The presence of full-length complements of viral genomic RNA is a hallmark of RNA virus replication within an infected cell. As such, methods for detecting and measuring specific strands of viral RNA in infected cells and tissues are important in the study of RNA viruses. Strand-specific quantitative real-time PCR (ssqPCR) assays are increasingly being used for this purpose, ...

In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site ...

These emulations allow accurate predictions and uncertainty quantification. Project Administrator(s): MISTY DAVIES. Members. MISTY DAVIES � Indir Jaganjac.

Accurate quantification of the amounts of trace gases and particulate matter emitted from vegetation

Significant figures shown to clarify magnitudes. .... validation, and uncertainty quantification. Verification is the process of determining that a ...

... Cruz to perform statistical emulation of complex, nonlinear simulators at NASA. These emulations allow accurate predictions and uncertainty quantification. ...

BackgroundThe BALB/c mouse is commonly used to study RSV infection and disease. However, despite the many advantages of this well-characterised model, the inoculum is large, viral replication is restricted and only a very small amount of virus can be recovered from infected animals. A key question in this model is the fate of the administered virus. Is replication really being measured or is the ...

INTRODUCTION Conventional Illumina RNA-Seq does not have the resolution to decode the complex eukaryote transcriptome due to the lack of RNA polarity information. Strand-specific RNA sequencing (ssRNA-Seq) can overcome these limitations and as such is better suited for genome annotation, de novo transcriptome assembly, and accurate digital gene expression ...

Quantitative real-time PCR (QPCR) was used to quantify the genome of Solenopsis invicta virus-2 (SINV-2) from infected individual ants of S. invicta. Strand-specific cDNA synthesis oligonucleotide primers and RNase digestion after cDNA synthesis allowed quantification of plus (genomic) and minus (replicative) strands of the SINV-2 genome. Both strands were ...

for in vivo fat quantification, where rapid abdominal imaging during breath-holds is essential. Results from for accurate abdominal fat quantification on water-saturated magnetic resonance images. J Magn Reson Imag- ingOriginal Research Quantification of Absolute Fat Mass Using an Adipose Tissue Reference Signal

The primary of this project are the development of (1) a general stochastic model for DNA sequencing errors (2) algorithms to restore the original DNA sequence and (3) statistical methods to assess the accuracy of this restoration. A secondary objective is to develop new algorithms for fragment assembly. Initially a stochastic model that assumes errors are independent and uniformly distributed ...

The goal for the SAMEX magnetograph's optical system is to accurately measure the polarization state

... of the Upper Volga River, Russia: what complex mixture of organics and metals are present? Eutrophic conditions ... ...