Site-specifically modified 2?-methylseleno RNA represents a valuable derivative for phasing of X-ray crystallographic data. Several successful applications in three-dimensional structure determination of nucleic acids, such as the Diels�Alder ribozyme, have relied on this modification. Here, we introduce synthetic routes to 2?-methylseleno phosphoramidite building blocks of ...
PubMed Central
. Phosphoramidites 4 and 8 were prepared and used in solid phase syntheses of RNA oligomers con- taining the sequence synthesis using standard solid-phase chemistry (9), the RNA was cleaved off the solid support and the baseChemical syntheses of inhibitory substrates of the ...
E-print Network
Solid-phase enzyme immunoassay (EIA) was developed for detecting natural antibodies to angiotensine-converting enzyme (ACE). Optimal conditions for detecting natural anti-ACE by EIA in the sera of donors and patients with disorders of arterial pressure are selected. The findings indicate that the level of natural ...
PubMed
solid-phase synthesis. The random region* Corresponding author. Cell. Mol. Life Sci. 65 (2008) 2156 oligonucleotides during solid-phase syn- thesis, generating appropriately modified deoxyribo- zymes in large, individual deoxyribo- zymes have been identified that catalyze RNA cleavage, RNA ligation, and a growing
Solid phase selection Ligation PCR amplification Detection on a universal index array address universal along with biotinylated oligo-dT. (2) Solid phase selection: The mix is then transferred ligation-dependent hybridization approach (Fig 1), RASL (RNA Annealing Selection and Ligation), has ...
Growth of Escherichia coli on acetate as the sole source of carbon and energy requires operation of the glyoxylate bypass in connection with the expression of the polycistronic ace operon. The structural organization of this operon is presented, including the 3 structural genes coding respectively for malate synthase (aceB), isocitrate lyase ...
RNA has been immobilized in a manner suitable for use in molecular hybridization experiments with dissolved RNA or DNA by a nonaqueous solid-phase reaction with carbonyldiimidazole and RNA �dry coated� on cellulose or, preferably, on previously activated phosphocellulose filters. Immobilization of ...
triplet ligation. All oligonucleotideswere synthesized using solid-phase phosphoramidite chemistry synthesizedasdescribed (Doudnaet al., 1990). RNA and DNA oligonucleotideswere made by solid-phase synthesis-directed ligation of RNA oligonucleotides (Doudna et al., 1989, 1991). We have sought to develop a more general ...
... RAPID SOLID-PHASE RADIOIMMUNOASSAY FOR STAPHYLOCOCCAL ENTEROTOXIN A ... Rapid Solid-Phase Radioimmunoassay for ...
DTIC Science & Technology
... Accession Number : ADD423943. Title : Manipulating Macromolecules in the Solid Phase (Solid-Phase Forming),. Corporate Author : ...
The pinewood nematode genome encodes at least three distinct acetylcholinesterases (AChEs). To understand physiological roles of the three pinewood nematode AChEs (BxACE-1, BxACE-2, and BxACE-3), BxACE-3 in particular, their tissue distribution and inhibition profiles were investigated. Immunohistochemistry ...
Mammalian angiotensin-converting enzyme (ACE) exists as two forms, somatic (sACE), controlling blood pressure via angiotensin II, and testicular (tACE), whose function is unknown. The former has two highly homologous N- and C-terminal Zn2+ metallopeptidase active sites, whereas the latter only has one, which is identical to the ...
1. The role of angiotensin-converting enzyme (ACE) 2 is likely to balance the status of the renin-angiotensin system (RAS) by degrading angiotensin (Ang) II and generating Ang-(1-7). Earlier demonstrations that ACE2 is insensitive to ACE inhibitors prompted us to evaluate the effect of ACE inhibitors on ...
Previous studies suggest that while lung angiotensin converting enzyme (ACE) activity is reduced during chronic hypoxia, inhibitors of ACE attenuate hypoxic pulmonary hypertension. In an attempt to explain this paradox we investigated the possibility that whole lung ACE activity may not reflect local pulmonary vascular ...
Adipose tissue expresses components of the renin-angiotensin system (RAS). Angiotensin converting enzyme (ACE2), a new component of the RAS, catabolizes the vasoconstrictor peptide ANG II to form the vasodilator angiotensin 1-7 [ANG-(1-7)]. We examined whether adipocytes express ACE2 and its regulation by manipulation of the RAS and by high-fat (HF) ...
A new robust high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS)-based screening method for angiotensin-converting enzyme (ACE)-inhibiting substances in crude samples is described. The ACE assay is carried out in a typical offline setup by incubation of the samples with ACE and angiotensin I ...
BackgroundACE2 is a novel homologue of angiotensin converting enzyme (ACE). ACE2 is highly expressed in human heart and animal data suggest that ACE2 is an essential regulator of cardiac function in vivo. Since overactivity of the renin-angiotensin system contributes to the progression of heart failure, this ...
Angiotensin-converting enzyme (ACE) is a zinc metallopeptidase that plays a major role in blood homoeostasis and reproduction in mammals. In vertebrates, both transmembrane and soluble ACE, containing one or two homologous active sites, have been characterized. So far, several ACEs from invertebrates have been cloned, but only in ...
Two genes (ace-1 and ace-2) encode two major classes (A and B) of acetylcholinesterase (AChE) in the nematode Caenorhabditis elegans. A null mutation in ace-1 (allele p1000) suppresses all acetylcholinesterase activity of class A. We have identified an opal mutation TGG (W99)-->TGA (Stop) as the only alteration in the mutated gene. ...
Automated solid-phase DNA sequencing was used for determination of partial 16S ribosomal DNA sequences of mycoplasmas. The sequence information was used to establish phylogenetic relationships of 11 different mycoplasmas whose 16S rRNA sequences had not been determined earlier. A biotinylated fragment corresponding to positions 344 to 939 in the ...
GFP-C{kappa} fusion protein was previously shown selectable on ribosome display platform with solid phase antibodies against GFP determinant [Y.-M. Yang, T.J. Barankiewicz, M. He, M. Taussig, S.-S. Chen, Selection of antigenic markers on a GFP-C{kappa} fusion scaffold with high sensitivity by eukaryotic ribosome display, Biochem. Biophys. Res. Commun. 359 ...
Energy Citations Database
Human monocytes/macrophages express the angiotensin-converting enzyme (ACE) but nothing is known about its role under physiological conditions. As adipose tissue contains resident macrophages that have been implicated in the generation of insulin resistance in expanding fat mass, we determined whether adipocytes release factors that affect ACE expression ...
Angiotensin I-converting enzyme (ACE) is a key enzyme in the regulation of systemic blood pressure and plays a major role in the renin-angiotensin and bradykinin-kinin systems, at the luminal surface of the vascular endothelia. To identify the promoter region, the transcription regulatory elements and the cell specificity of the ACE gene, five successive ...
deoxyribozyme ligation activities using DNA from solid-phase synthesis, the general approach has been described oligonucleotides were prepared by solid-phase synthesis at IDT (Coralville, IA). RNA transcripts were prepared strand was prepared by solid-phase synthesis at IDT. The ribozyme enzyme strand was prepared by in vitro
This book contains 36 papers. Some of the titles are: Site-specific cleavage of DNA at 8-, 9-, and 10-bp sequences; The use of DNA methylases to alter the apparent recognition specificities of restriction endonucleases; Random cloning and sequencing by the M13/Dideoxynucleotide chain termination method; Solid-phase methods for sequencing of oligodeoxynucleotides and DNA; and ...
, 46, 7420 � 7424. [21] Preparation of the DNA-peptide-DNA conjugates by solid- phase synthesis-catalyzed activity;[8,9] our laboratory has focused on RNA ligation.[7,10] Deoxyribozymes that phosphorylate or cap DNA oligonu- cleotides using ATP have been identified,[11] as have DNA enzymes that ligate, cleave
