Coenzyme A (CoA) and its thioester derivatives are important precursor molecules for many industrially useful compounds such as esters, PHBs, lycopene and polyketides. Previously, in our lab we could increase the intracellular levels of CoA and acetyl-Coenzyme A (acetyl-CoA) by overexpressing one of the upstream rate-controlling enzymes pantothenate kinase with a concomitant ...
PubMed
Isoamyl acetate, produced via fermentation, is a natural flavor chemical with applications in the food industry. Two alcohol acetyltransferases from Saccharomyces cerevisiae (ATF1 and ATF2) can catalyze the esterification of isoamyl alcohol with acetyl coenzyme A. The respective genes were cloned and expressed in an appropriate ...
An in vivo method of producing isoamyl acetate and succinate simultaneously has been developed in Escherichia coli to maximize yields of both high value compounds as well as maintain the proper redox balance between NADH and NAD(+). Previous attempts at producing the ester isoamyl acetate ...
Volatile aroma-active esters are responsible for the fruity character of fermented alcoholic beverages such as beer and wine. Esters are produced by fermenting yeast cells in an enzyme-catalyzed intracellular reaction. In order to investigate and compare the roles of the known Saccharomyces cerevisiae alcohol acetyltransferases, Atf1p, Atf2p and Lg-Atf1p, ...
PubMed Central
The fruity odours of wine are largely derived from the synthesis of esters and higher alcohols during yeast fermentation. The ATF1- and ATF2-encoded alcohol acetyltransferases of S. cerevisiae are responsible for the synthesis of ethyl acetate and isoamyl acetate esters, while the EHT1-encoded ...
... the mass flow meter. 4.4 Results. Olfactory Substances. Ammonia, isoamyl acetate (banana oil), menthol, ... APPENDIX 50 Page 53. Isoamyl Acetate. ...
DTIC Science & Technology
Although the bacterium E. coli is chosen as the host in many bioprocesses, products derived from the central aerobic metabolic pathway often compete with the acetate-producing pathways poxB and ackA-pta for glucose as the substrate. As such, a significant portion of the glucose may be excreted as acetate, wasting substrate that could have otherwise been ...
The gene coding for alcohol acetyltransferase ( ATF2), which catalyzes the esterification of isoamyl alcohol and acetyl coenzyme A (acetyl-CoA), was cloned from Saccharomyces cerevisiae and expressed in Escherichia coli. This genetically engineered strain of E. coli produced the ester isoamyl acetate when ...
... Challenges will be performed only with isoamyl acetate (approved for usage with human subjects by the Office of the US Army Surgeon General). ...
Isoamyl acetate is synthesized from isoamyl alcohol and acetyl coenzyme A by alcohol acetyltransferase (AATFase) in Saccharomyces cerevisiae and is hydrolyzed by esterases at the same time. We hypothesized that the balance of both enzyme activities was important for optimum production of isoamyl ...
The distinctive flavor of wine, brandy, and other grape-derived alcoholic beverages is affected by many compounds, including esters produced during alcoholic fermentation. The characteristic fruity odors of the fermentation bouquet are primarily due to a mixture of hexyl acetate, ethyl caproate (apple-like aroma), iso-amyl ...
... Purity Ethyl Alcohol Absolute Isoamyl Acetate CP Ethyl Ether ... to 10 mm at 35,2 °C^V Isoamyl acetate and n-amyl acetate have about ...
... 5 2.1.1 Isoamyl Acetate Test 6 ... The most common qualitative fit tests (Hardis et al, 1983) are isoamyl acetate, irritant smoke or sodiuz, saccharin. ...
... t 1.0 - Toluene extraction isoamyl acetate (Hattie et al. 1991) 6.0 0.3 0.06 0.04 0.003 ... two methods employ toluene and isoamyl acetate as ...
An analytical procedure for the separation and quantification of ethyl acetate, ethyl butyrate, ethyl hexanoate, ethyl lactate, ethyl octanoate, ethyl nonanoate, ethyl decanoate, isoamyl octanoate, and ethyl laurate in cachaca, rum, and whisky by direct injection gas chromatography-mass spectrometry was developed. The analytical method is simple, ...
... with potassium acetate. After centrifugation, the plasmid DNA in the supernatant fluid was extracted with phenol-chloroform- isoamyl alcohol to ...
... Some of the more widely used challenge materials are Iritant smoke, sodium saccharin, and isoamyl acetate vapors. Qualitative ...
... bottle. After dehydration, these samples were treated with isoamyl acetate for 60 min and dried at the critical ... ...
NBII National Biological Information Infrastructure
... function of wavelength. The collodion films were prepared by dissolving aged collodion in isoamyl acetate. The films, formed ...
The resistance of chlorosulfonated polyethylene geomembranes to nine aliphatic esters viz., methyl acetate, ethyl acetate, methyl acetoacetate, n-butyl acetate, diethyl oxalate, iso-amyl acetate, diethyl malonate, and diethyl succinate was investigated in the temperature ...
Energy Citations Database
The aromatic composition and key odorants of young red wines produced from Cabernet Sauvignon, Cabernet Franc, and Cabernet Gernischet wines were compared and the reasons for the difference in their aromatic compounds were discussed. Forty-three odorants were detected in Cabernet Sauvignon and Cabernet Franc wines compared to 50 in Cabernet Gernischet wine. Quantitatively, acids formed the most ...
...containing 0.1 ±0.025 percent isoamyl acetate vapor, and the intake of the...hose length will be located in isoamyl acetate-free air. (b) The man in the isoamyl acetate atmosphere will draw his...
Code of Federal Regulations, 2010
...containing 0.1 ±0.025 percent isoamyl acetate vapor, and the blower, the...hose length will be located in isoamyl acetate-free air. (b) The man in the isoamyl acetate atmosphere will draw his...
...chamber containing 100 p.p.m. isoamyl acetate vapor for a half-mask facepiece and 1,000 p.p.m. isoamyl acetate vapor for a full facepiece or...shall not detect the odor of isoamyl acetate during the...
The wine yeast Saccharomyces cerevisiae is central in the production of aroma compounds during fermentation. Some of the most important yeast-derived aroma compounds produced are esters. The esters ethyl acetate and isoamyl acetate are formed from alcohols and acetyl-CoA in a reaction catalysed ...
Thirty-seven basidiomycetous yeasts belonging to 30 species of seven genera were grown on media containing l-cysteine or l-methionine as sole nitrogen sources with the objective of evaluating volatile organic sulfur compound (VOSC) production. The headspace of yeast cultures was analyzed by the solid-phase microextraction (SPME) sampling method, and volatile compounds were quantified and ...
Coenzyme A (CoA) and its thioester derivative acetyl-Coenzyme A (acetyl-CoA) participate in over 100 different reactions in intermediary metabolism of microorganisms. Earlier results indicated that overexpression of upstream rate-limiting enzyme pantothenate kinase with simultaneous supplementation of precursor pantothenic acid to the culture media increased intracellular CoA levels significantly ...
1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, strongly inhibited the growth of sake yeast at 120 microM in YPD medium, whereas at 30 microM it reduced cellular production of isoamyl acetate to 20% of the control level despite the absence of inhibitory effect on CO2 evolution. The FPy-resistant mutant A1 was characterized by the high ...
May 14, 2001 ... Acetic and propionic acid and isoamyl alcohol. Isovaleric acid ... Isovaleric acid. Acetic, propionic and butyric acid ...
NASA Website
In the present study, a simple and sensitive methodology based on dynamic headspace solid-phase microextraction (HS-SPME) followed by thermal desorption gas chromatography with quadrupole mass detection (GC-qMSD), was developed and optimized for the determination of volatile (VOCs) and semi-volatile (SVOCs) compounds from different alcoholic beverages: wine, beer and whisky. Key experimental ...
... extracted with phenol/chloroform/isoamyl alcohol and chloroform/isoamyl alcohol and precipitated with sodium acetate and ethanol (Sambrook, Fritch, and Maniatis 1989). The ... ...
... with phenol, phenol/ chloroform/isoamyl alcohol, and chloroform/isoamyl alcohol, followed by precipitation with 3-M sodium acetate and 100% ethanol. Genomic DNA was resuspended in ... ...
... half chloroform isoamyl 23:1), and finally chloroform-isoamyl alcohol (23:1). DNA was precipitated with 1/10 volume 3M sodium acetate and 1 volume isopropanol and stored at �20° ... ...
Lactic acid bacteria (LAB) were isolated from Korean Meoru (Vitis coigneties) wine and identified as Lactobacillus plantarum meoru0711 (KACC 91436C). The fermentative behavior and metabolic effects of L. plantarum during malolactic fermentation (MLF) were compared with those of the commercial Oenococcus oeni strain through 1H NMR- and GC-based metabolic profiling. Twenty-two primary metabolites of ...
Glycerol triacetate was successfully used as a green solvent and as the acyl donor in the transesterification of isoamyl alcohol to produce isoamyl acetate using free and immobilized Candida antarctica lipase B. Immobilized lipase was more catalytically active than free lipase and could be easily separated from the reaction mixture by ...
A novel support has been utilized for immobilization of lipase, which was prepared by amination of silica with ethanolamine followed by cross linking with glutaraldehyde. Lipases from Rhizopus oryzae 3562 and Enterobacter aerogenes were immobilized on activated silica gel, where they retained 60 and 50% of respective original activity. The thermal stability of the immobilized lipases was ...
Distribution of Mo and W between hydrobromic acid and ionic acid- containing organic solvents was studied. It was found that with increased acid the extraction of W decreases and the extraction of Mo increases. Extraction of W from 1.0 n hydrobromic solution by given solvents increases accordingly; isoamyl acetate isoamyl ...
The impact of mixed cultures of Hanseniaspora osmophila and Saccharomyces cerevisiae with different initial yeast ratios on wine composition has been examined. The mixed culture significantly affected sugar consumption, the main enological parameters and ester concentrations, with the exception of glycerol, isoamyl acetate and diethyl ...
... isoamyl and amyl alcohols, 2-phenylethanol, and other carboxylic acids and esters. In other triatomine species, no additional analyses of ... ...
... 50%, 75%, 80%, 95%, and 99.9%) and isoamyl acetate (100%) (10 min each step), and finally dried ... ...
... gas respirator for protection against organic vapors, will enter an atmosphere containing 0.01 percent of volume (100 ppm)of isoamyl acetate vapor. ...
Jan 17, 2011 ... The MIT 2006 iGEM team created a system to express methyl salicyate, a mint smelling compound, and isoamyl acetate (banana scent) ...
... move the last traces of isoamyl alcohol from XIVa at 600 and 1-mm pressure, ... mony(III) oxide and calcium acetate dihydrate under nitrogen at 2006C ...
... the acetone in the specimens was replaced with isoamyl acetate. After being dried at a critical point and ... ...
... The ethanol is then replaced in IO-min steps with increasinL contentrations of isoamyl acetate (50%, 75%, 95%, 100%, 100%). - , Page 5. ...
... nts: Dimethyl SulfoxideL. Triethyl Phosphite. Bis (2-hthylhexyl) Sebacate. Isoamyl Acetate, Dipropyl Sulfane. Sulfur Hexafluoride ...
... were treated in a graded ethanol series and isoamyl acetate, dried in a critical point dryer (Hitachi SCP- ... ...
... phe- nol: chloroform: isoamyl alcohol. The DNA was precipi- Materials and methods tated from the aqueous phase with sodium acetate and two ...
... by passing air through an aqueous suspension of Isoamyl acetate (50 μl in 50 ml of distilled water) ... ...
... Paralleled by an Increase in Olfactory Sensitivity to Isoamyl Acetate. Chemical Senses 34:8, 667-678Online publication date: ... ...
... through a graded ethanol series. After treatment with isoamyl acetate, the specimens were critical-point dried, gold-coated ... ...
... time with an equal volume of chloroform/isoamyl alcohol (24:1). The aqueous phase was precipitated with 0.1 volume of 2 M sodium acetate and 2 ...
... series and treated for 20 sec with 100% isoamyl acetate. They were quickly transferred to a Hitachi HCP- ... ...
... graded series of ethanol solutions. After immersion in isoamyl acetate, they were dried in a critical point apparatus ( ... ...
... tetroxide, dehydrated in graded ethanol solution, keep in isoamyl acetate overnight, and critical-point dried in CO2, coated ... ...
... ultimately replaced through two 30 min baths of isoamyl acetate. Eggs were then dried in a CO2 critical- ... ...
... extraction of organic compounds from water. could be obtained using isoamyl acetate. Unfortu- Anspach et al. (1982) evaluated 13 commercially ...
... The fit of each mask will be evaluated by exposing the participants te isoamyl acetate (banana oil) fumes after the mask has been donned. ...
... dehydrated through a graded ethanol series, transferred to isoamyl acetate and finally dried by using a critical point ... ...
... graded series of ethanol, and then exchanged with isoamyl acetate. The specimens were critical-point dried (Hitachi HCP- ... ...
... Page 12. Three qualitative respirator fit tests: the negative pressure test, the isoamyl acetate test, aad the irritant smoke test were performed concur-- ...
... the order of 3000 which represents a potentially significant improvement over the protection factor provided by isoamyl acetate (banana oil), which ...
... water solution, dehydrated in ethanol series, replaced in isoamyl acetate, dried through the CO2 critical point, and viewed ... ...
An extracellular thermostable lipase from Amycolatopsis mediterranei DSM 43304 has been purified to homogeneity using ammonium sulphate precipitation followed by anion exchange chromatography and hydrophobic interaction chromatography. This protocol resulted in a 398-fold purification with 36% final recovery. The purified A. mediterranei DSM 43304 lipase (AML) has an apparent molecular mass of 33 ...
...wearer will enter a chamber containing 100 p.p.m. isoamyl acetate vapor for half-mask facepieces, and 1,000 p... (4) Each wearer shall not detect the odor of isoamyl-acetate vapor during the...
Branched-chain alcohols, such as isoamyl alcohol and isobutanol, and isoamyl acetate are important flavor components of yeast-fermented alcoholic beverages. Analysis of a null mutant of the BAT2 gene encoding cytosolic branched-chain amino acid aminotransferase, and a transformant with multi-copy plasmids containing the BAT2 gene ...
A new direct conversion of O,O-acetals to esters via O,P-acetal intermediates was developed. The regioselective cleavage of unsymmetrical cyclic acetals occurred to give the more crowded esters as single isomers. PMID:21713294
Draft technical standards are outlined for sec-hexyl acetate, isoamyl acetate, isobutyl acetate, methyl acetate, methyl acrylate, methyl cellosolve acetate, methyl methacrylate and n-propyl acetate. Each standard includes definitions, monitoring ...
National Technical Information Service (NTIS)
A diploid yeast strain D61.M was used to study induction of mitotic chromosomal malsegregation, mitotic recombination and point mutation. Several ketones (including acetone and methyl ethyl ketone) and some organic acid esters (including the methyl, ethyl and 2-methoxyethyl esters of acetic acid) and acetonitrile strongly induced ...
... Methyl hexyl carbinol 26.5 dynes/cm Isoamyl alcohol 24.1 11 If Tert ... acetone , amyl acetate , cel losolve Acetate , ethyl acetoacetate , butyl or piropyl ...
please delete the no. 721012:007211Complex saits of uranyl nitrate with diethyl ether, ethyl acetate, acetone, nitromethane, and methyl alcohol were obtained, as well as the uranyl acetate wdth methyl, ethyl, and isoamyl alcohol in the crystalline lattice. Their luminescence spectra were studied. (tr-auth)
Aromatic compounds are an important element in the flavor of yeast-fermented alcohol. We isolated mutants of Saccharomyces cerevisiae capable of growth at high levels of hydrostatic pressure. Among them, the HPG1 mutants, with a defect in their Rsp5 ubiquitin ligase, were found to produce high amounts of aromatics due to enhanced leucine uptake, with isoamyl alcohol production ...
... HYDROLYSIS, *ESTERS, ACCELERATION, ENZYMES, SUBSTRATES, CATALYSIS, PHENOLS, HYDROXIDES, ACETATES, HYDROXYL ...
The yeast alcohol acetyl transferase I, Atf1p, is responsible for the major part of volatile acetate ester production in fermenting Saccharomyces cerevisiae cells. Some of these esters, such as ethyl acetate and isoamyl acetate, are important for the fruity flavours of ...
Hanseniaspora guilliermondii and Hanseniaspora uvarum were tested in grape must fermentations as pure and mixed starter cultures with Saccharomyces cerevisiae. In pure cultures, the specific growth rates found were 0.29 h(-1) for H. uvarum, 0.23 h(-1) for H. guilliermondii and 0.18 h(-1) for S. cerevisiae. No significant differences were observed between these values and those obtained in mixed ...
... Title : Preliminary Studies on The Crosslinking of Poly(ester-acetals) and Poly(amide-acetals) on Glass Substrates,. Corporate Author : ...
The effects of nitrogen addition into nitrogen deficient/depleted media on the release of aroma compounds post-fermentation were investigated in three commercial yeast strains of Saccharomyces cerevisiae which highlight the yeast strain effect as well as nitrogen effects. By comparing the two timings of nitrogen addition, prior to fermentation or later at stationary phase (72 h), it was shown that ...
The effects of three fungicide residues (cyprodinil, fludioxonil, and pyrimethanil) on the aromatic composition (acids, alcohols, and esters) of Vitis vinifera white wines (var. Air�n) inoculated with three Saccharomyces cerevisiae strains (syn. bayanus, cerevisiae, and syn. uvarum) are studied. The aromatic exponents were extracted and concentrated by adsorption-thermal ...
In this contribution are presented the fermentations of the main substrates present in a decaying corpse, namely carbohydrates, amino acids, glycerol and fatty acids, generating the post-mortem volatile compounds that could be detected along with ethanol during the forensic ethanol analysis. The available literature (preferably reviews) on microbial metabolic pathways (enzymes, substrates, ...
We investigated the influence of the fermenter size on alcoholic fermentation. Experiments were carried out at pilot scale, in 100-L fermenters, and at laboratory scale, in stirred and static 1-L fermenters. Two musts, Grenache blanc and Sauvignon, were fermented with and without the addition of solid particles from grape musts. Highly clarified must fermentation kinetics was strongly affected by ...
The effect of pure and mixed fermentation by Saccharomyces cerevisiae and Hanseniaspora valbyensis on the formation of major volatile components in cider was investigated. When the interaction between yeast strains of S. cerevisiae and H. valbyensis was studied, it was found that the two strains each affected the cell growth of the other upon inoculation of S. cerevisiae during growth of H. ...
The effects of volatile compounds produced during coffee processing by Pichia anomala, P. kluyveri and Hanseniaspora uvarum on growth of Aspergillus ochraceus and production of ochratoxin A (OTA) were studied. On malt extract agar (MEA) and on coffee agar (CA), exposure of A. ochraceus to the gaseous phase of malt yeast glucose peptone (MYGP) plates inoculated with P. anomala, P. kluyveri and H. ...
... phenol�:�chloroform/isoamyl alcohol (24:1), and chloroform�:�isoamyl alcohol (24:1). DNA was precipitated by using 0.2 volumes of 10 M ammonium acetate and 1 volume of 95% ethanol. DNA yield ... ...
Distribution coefficients of Li isotopes equilibrated between the lithium salts in aqueous solutions and Li salts in acetone, isoamyl, alcohol, diethyl ether, and amyl acetate were determined. Additions of a light petroleum fraction to lithium chloride solution in mixed methylamine and isoamyl alcohol in contact with aqueous ...
The release of isoamyl acetate from starch-based matrices was studied on the basis of a cumulative dynamic headspace analysis. Two corn starches were compared, which are known to yield pastes and gels differing in their structures and properties. These properties were assessed by viscometry and viscoelastic measurements. Aroma release was discussed as a ...
The objective of this work was to propose a reaction mechanism and to develop a rate equation for the synthesis of isoamyl acetate by acylation of the corresponding alcohol with acetic anhydride using the lipase Novozym 435 in n-hexane. The reaction between isoamyl alcohol and acetic anhydride ...
Ethyl, propyl and isopropyl 2-cyanoacrylate monomers appear to be a possible candidate as quick-setting adhesives for the adhesion of wet regnerated cellulose dialysis membranes. Of these, isopropyl or isoamyl 2-cyanoacrylate seem to warrant further inves...
... y ) Impregnation according to Steiner et al: With 0.15 m sodium acetate solution (5 min.). ... 1 0 Ethanol (1) (h Rf values of the DNB of Isoamyl-41 and ...
... then lightly homogenized and extracted with phenol/chloroform/isoamyl alcohol. After precipitation with sodium acetate and isopropanol, total DNA was quantified spectophotometrically based ... ...
... The digest was deproteinized by successive phenol/chloroform/isoamyl alcohol extractions and recovered by precipitation with 100% ethanol in 7.5 M ammonium acetate. The DNA was dissolved in Tris-EDTA buffe...
... min. The extraction is repeated with phenol/chloroform/isoamyl alcohol and the DNA precipitated by adding 0.1 volumes of 3 M sodium acetate, pH 5.3, and 2 volumes of absolute ... ...
... n-hexane; isoamyl alcohol, n-amyl alcohol, sec-butanol, 2-heptanol, n-butyl carbinol; ethyl lactate, butyl cellosolve, ethyl formate, isopropyl acetate, ...
Draft technical standards are outlined for hydroquinone, isoamyl alcohol, isobutyl alcohol, isopropyl alcohol, isopropyl acetate, methyl alcohol, methyl isobutyl carbinol, and propyl alcohol. Each standard includes definitions, monitoring requirements, me...
... 25:24:1), and once with chloroform:isoamyl alcohol (25:1). Plasmid DNA was precipitated by addition of 0.1 volume of 3 M sodium acetate pH 6.5 ...
... 25:24:1) and once with chloroform:isoamyl alcohol (25:1). Plasmid DNA was precipitated by adding 0.1 volume of 3M sodium acetate pH 6.5 and 2 ...
... Since the 14C-Acetate is organic, it partitions into the organic ... 400 ml isoamyl alcohol (Mallinckrodt #2992070) 17.2g PPO (Amersham #190020) 1.0 ...
Acetic and propionic acid and. Prevotella. Bac 5. Isovaleric acid. 65 isoamyl alcohol ... isovaleric and isocaproic acid. Isovaleric acid. *This is a fungus ...
... '0.8 1.5 0.8 50 ms (a) 76.5% Lead Mononitrorescorcinate 8.5% Potassium Chlorate 15.0% 1/2 Sec. Nitrocotton, in Iso-Amyl Acetate ...
... mixed with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 PCI). The homogenate was cooled, and 30 μl of 8 M potassium acetate was added to precipitate the protein. After incubation ... ...
... at 14,000 rpm. 10 μl of potassium acetate (8M) were added to the extract before centrifugation. The aqueous phase was recovered and re-extracted with an equal volume of a chlorophorm- isoamyl alcohol mixtu...
... two solvent systems: (1) isoamyl acetate:propionic acid:1-propanol:water 4:3:2:1 (v/v) (Hofmann 1962); and (2) a double ... ...
... The anion would then be treated with Isoamyl nitrite to form the c ... showed no ovidanco of deburizyistion using Pd/C In ethyl acetate, methanol, or ...
... Sodium acetate, pH 4.0, was added to yield a final concentra- t ... of water saturated phenol and one fifth volume of chloroform:isoamyl alcohol (49:1 ...
... (a) Each apparatus will be tested for tightness by persons wearing it in an atmosphere of 1,000 p.p.m. isoamyl acetate. (b) Six persons will each wear the apparatus in the test concentrations specified in paragraph (a) of...
A preliminary investigation of the preparation and properties of cellulose nitrate-acetate mixed esters was conducted. These mixed esters can be prepared in a wide range of amounts of substitution of both acetate and nitrate groups either through acetylat...
In order to elucidate the aroma components of wine produced in the Loess Plateau region of China, volatile compounds of young wines from Cabernet Sauvignon, Cabernet Gernischet and Chardonnay varieties grown in the new ecological region were investigated for the first time in this research. Among the volatile compounds analyzed by HS-SPME with GC-MS, a total of 45, 44 and 42 volatile compounds ...
The reactions of several ethyl phosphonate esters with mercuric acetate gave the corresponding bis(acetoxymercuri) phosphonates. Various reactions of these compounds are reported.
... Title : Nitrated and/or Fluorinated Ethyl Amino Acetic Acids and Esters ... Abstract : This invention is to provide carboxylic acid esters that can be ...
... mixed esters can be prepared in a wide range of amounts of substitution of both acetate and nitrate groups either through acetylation of cellulose ...
... Accession Number : ADD427366. Title : Azelaaldehydic Acid Ester-Acetal Derivatives as Plasticizers for Poly(Vinyl Chloride). ...
... For ethyl-d5 acetate, the spectrum is distinguishable from the spectrum of a symmetrically bonded acetate ion. (Author). ...
The Russian patent concerns a method for obtaining esters of difluoroaminoacetic acid. It is distinguished by the fact that the ester of acetic acid is treated with tetrafluorohydrazine in a medium of an inert solvent with heating. The process is carried ...