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1
Applicability of CoA/acetyl-CoA manipulation system to enhance isoamyl acetate production in Escherichia coli.
2004-10-01

Coenzyme A (CoA) and its thioester derivatives are important precursor molecules for many industrially useful compounds such as esters, PHBs, lycopene and polyketides. Previously, in our lab we could increase the intracellular levels of CoA and acetyl-Coenzyme A (acetyl-CoA) by overexpressing one of the upstream rate-controlling enzymes pantothenate kinase with a concomitant ...

PubMed

2
Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients.
2007-09-21

Isoamyl acetate, produced via fermentation, is a natural flavor chemical with applications in the food industry. Two alcohol acetyltransferases from Saccharomyces cerevisiae (ATF1 and ATF2) can catalyze the esterification of isoamyl alcohol with acetyl coenzyme A. The respective genes were cloned and expressed in an appropriate ...

PubMed

3
Metabolic engineering of the anaerobic central metabolic pathway in Escherichia coli for the simultaneous anaerobic production of isoamyl acetate and succinic acid.

An in vivo method of producing isoamyl acetate and succinate simultaneously has been developed in Escherichia coli to maximize yields of both high value compounds as well as maintain the proper redox balance between NADH and NAD(+). Previous attempts at producing the ester isoamyl acetate ...

PubMed

4
Expression Levels of the Yeast Alcohol Acetyltransferase Genes ATF1, Lg-ATF1, and ATF2 Control the Formation of a Broad Range of Volatile Esters
2003-09-01

Volatile aroma-active esters are responsible for the fruity character of fermented alcoholic beverages such as beer and wine. Esters are produced by fermenting yeast cells in an enzyme-catalyzed intracellular reaction. In order to investigate and compare the roles of the known Saccharomyces cerevisiae alcohol acetyltransferases, Atf1p, Atf2p and Lg-Atf1p, ...

PubMed Central

5
The effect of increased yeast alcohol acetyltransferase and esterase activity on the flavour profiles of wine and distillates.
2006-07-15

The fruity odours of wine are largely derived from the synthesis of esters and higher alcohols during yeast fermentation. The ATF1- and ATF2-encoded alcohol acetyltransferases of S. cerevisiae are responsible for the synthesis of ethyl acetate and isoamyl acetate esters, while the EHT1-encoded ...

PubMed

6
A Vapor Challenge Method of Measuring the Residual Life of ...
1990-08-01

... the mass flow meter. 4.4 Results. Olfactory Substances. Ammonia, isoamyl acetate (banana oil), menthol, ... APPENDIX 50 Page 53. Isoamyl Acetate. ...

DTIC Science & Technology

7
Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB pathways for the synthesis of isoamyl acetate.

Although the bacterium E. coli is chosen as the host in many bioprocesses, products derived from the central aerobic metabolic pathway often compete with the acetate-producing pathways poxB and ackA-pta for glucose as the substrate. As such, a significant portion of the glucose may be excreted as acetate, wasting substrate that could have otherwise been ...

PubMed

8
Production of isoamyl acetate in ackA-pta and/or ldh mutants of Escherichia coli with overexpression of yeast ATF2.
2003-10-28

The gene coding for alcohol acetyltransferase ( ATF2), which catalyzes the esterification of isoamyl alcohol and acetyl coenzyme A (acetyl-CoA), was cloned from Saccharomyces cerevisiae and expressed in Escherichia coli. This genetically engineered strain of E. coli produced the ester isoamyl acetate when ...

PubMed

9
Masks, Protective
1997-08-01

... Challenges will be performed only with isoamyl acetate (approved for usage with human subjects by the Office of the US Army Surgeon General). ...

DTIC Science & Technology

10
Balance of Activities of Alcohol Acetyltransferase and Esterase in Saccharomyces cerevisiae Is Important for Production of Isoamyl Acetate
1998-10-01

Isoamyl acetate is synthesized from isoamyl alcohol and acetyl coenzyme A by alcohol acetyltransferase (AATFase) in Saccharomyces cerevisiae and is hydrolyzed by esterases at the same time. We hypothesized that the balance of both enzyme activities was important for optimum production of isoamyl ...

PubMed Central

11
Effect of increased yeast alcohol acetyltransferase activity on flavor profiles of wine and distillates.
2000-02-01

The distinctive flavor of wine, brandy, and other grape-derived alcoholic beverages is affected by many compounds, including esters produced during alcoholic fermentation. The characteristic fruity odors of the fermentation bouquet are primarily due to a mixture of hexyl acetate, ethyl caproate (apple-like aroma), iso-amyl ...

PubMed

12
Effect of Increased Yeast Alcohol Acetyltransferase Activity on Flavor Profiles of Wine and Distillates
2000-02-01

The distinctive flavor of wine, brandy, and other grape-derived alcoholic beverages is affected by many compounds, including esters produced during alcoholic fermentation. The characteristic fruity odors of the fermentation bouquet are primarily due to a mixture of hexyl acetate, ethyl caproate (apple-like aroma), iso-amyl ...

PubMed Central

13
ELECTRON TRANSIT LAGS IN GEIGER COUNTERS
1953-06-01

... Purity Ethyl Alcohol Absolute Isoamyl Acetate CP Ethyl Ether ... to 10 mm at 35,2 °C^V Isoamyl acetate and n-amyl acetate have about ...

DTIC Science & Technology

14
Quantitative Respirator Fit Testing through Dynamic Pressure ...
2011-05-13

... 5 2.1.1 Isoamyl Acetate Test 6 ... The most common qualitative fit tests (Hardis et al, 1983) are isoamyl acetate, irritant smoke or sodiuz, saccharin. ...

DTIC Science & Technology

15
Improved Salting Out Extraction-Preconcentration Method for ...
1991-10-01

... t 1.0 - Toluene extraction isoamyl acetate (Hattie et al. 1991) 6.0 0.3 0.06 0.04 0.003 ... two methods employ toluene and isoamyl acetate as ...

DTIC Science & Technology

16
Quantitative ester analysis in cachaca and distilled spirits by gas chromatography-mass spectrometry (GC-MS).
2008-06-21

An analytical procedure for the separation and quantification of ethyl acetate, ethyl butyrate, ethyl hexanoate, ethyl lactate, ethyl octanoate, ethyl nonanoate, ethyl decanoate, isoamyl octanoate, and ethyl laurate in cachaca, rum, and whisky by direct injection gas chromatography-mass spectrometry was developed. The analytical method is simple, ...

PubMed

17
Host Factors Contributing to Disability Following Sulfur ...
1992-03-30

... with potassium acetate. After centrifugation, the plasmid DNA in the supernatant fluid was extracted with phenol-chloroform- isoamyl alcohol to ...

DTIC Science & Technology

18
Effect of Particle Size on Respirator Faceseal Leakage
1990-12-01

... Some of the more widely used challenge materials are Iritant smoke, sodium saccharin, and isoamyl acetate vapors. Qualitative ...

DTIC Science & Technology

19
Comparative Studies of the Genus Echinometra from Okinawa and Mauritius

... bottle. After dehydration, these samples were treated with isoamyl acetate for 60 min and dried at the critical ... ...

NBII National Biological Information Infrastructure

20
A Spectrophotometric Attachment for the Vacuum Ultraviolet
1961-12-01

... function of wavelength. The collodion films were prepared by dissolving aged collodion in isoamyl acetate. The films, formed ...

DTIC Science & Technology

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21
Interactions of chlorosulfonated polyethylene geomembranes with aliphatic esters: Sorption and diffusion phenomena
1995-07-01

The resistance of chlorosulfonated polyethylene geomembranes to nine aliphatic esters viz., methyl acetate, ethyl acetate, methyl acetoacetate, n-butyl acetate, diethyl oxalate, iso-amyl acetate, diethyl malonate, and diethyl succinate was investigated in the temperature ...

Energy Citations Database

22
Comparative study of aromatic compounds in young red wines from cabernet sauvignon, cabernet franc, and cabernet gernischet varieties in China.
2007-06-01

The aromatic composition and key odorants of young red wines produced from Cabernet Sauvignon, Cabernet Franc, and Cabernet Gernischet wines were compared and the reasons for the difference in their aromatic compounds were discussed. Forty-three odorants were detected in Cabernet Sauvignon and Cabernet Franc wines compared to 50 in Cabernet Gernischet wine. Quantitatively, acids formed the most ...

PubMed

23
42 CFR 84.161 - Man test for gases and vapors; Type B and Type BE respirators; test requirements.
2010-10-01

...containing 0.1 ±0.025 percent isoamyl acetate vapor, and the intake of the...hose length will be located in isoamyl acetate-free air. (b) The man in the isoamyl acetate atmosphere will draw his...

Code of Federal Regulations, 2010

24
42 CFR 84.160 - Man test for gases and vapors; Type A and Type AE respirators; test requirements.
2010-10-01

...containing 0.1 ±0.025 percent isoamyl acetate vapor, and the blower, the...hose length will be located in isoamyl acetate-free air. (b) The man in the isoamyl acetate atmosphere will draw his...

Code of Federal Regulations, 2010

25
42 CFR 84.124 - Facepiece tests; minimum requirements.
2010-10-01

...chamber containing 100 p.p.m. isoamyl acetate vapor for a half-mask facepiece and 1,000 p.p.m. isoamyl acetate vapor for a full facepiece or...shall not detect the odor of isoamyl acetate during the...

Code of Federal Regulations, 2010

26
Modulating aroma compounds during wine fermentation by manipulating carnitine acetyltransferases in Saccharomyces cerevisiae.
2006-11-29

The wine yeast Saccharomyces cerevisiae is central in the production of aroma compounds during fermentation. Some of the most important yeast-derived aroma compounds produced are esters. The esters ethyl acetate and isoamyl acetate are formed from alcohols and acetyl-CoA in a reaction catalysed ...

PubMed

27
Production of volatile organic sulfur compounds (VOSCs) by basidiomycetous yeasts.
2005-02-01

Thirty-seven basidiomycetous yeasts belonging to 30 species of seven genera were grown on media containing l-cysteine or l-methionine as sole nitrogen sources with the objective of evaluating volatile organic sulfur compound (VOSC) production. The headspace of yeast cultures was analyzed by the solid-phase microextraction (SPME) sampling method, and volatile compounds were quantified and ...

PubMed

28
Enhanced isoamyl acetate production upon manipulation of the acetyl-CoA node in Escherichia coli.

Coenzyme A (CoA) and its thioester derivative acetyl-Coenzyme A (acetyl-CoA) participate in over 100 different reactions in intermediary metabolism of microorganisms. Earlier results indicated that overexpression of upstream rate-limiting enzyme pantothenate kinase with simultaneous supplementation of precursor pantothenic acid to the culture media increased intracellular CoA levels significantly ...

PubMed

29
Improved production of isoamyl acetate by a sake yeast mutant resistant to an isoprenoid analog and its dependence on alcohol acetyltransferase activity, but not on isoamyl alcohol production.
2005-02-01

1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, strongly inhibited the growth of sake yeast at 120 microM in YPD medium, whereas at 30 microM it reduced cellular production of isoamyl acetate to 20% of the control level despite the absence of inhibitory effect on CO2 evolution. The FPy-resistant mutant A1 was characterized by the high ...

PubMed

30
(12) United States Patent - NASA Technical Report Server (NTRS)

May 14, 2001 ... Acetic and propionic acid and isoamyl alcohol. Isovaleric acid ... Isovaleric acid. Acetic, propionic and butyric acid ...

NASA Website

31
Development of a dynamic headspace solid-phase microextraction procedure coupled to GC-qMSD for evaluation the chemical profile in alcoholic beverages.
2008-01-08

In the present study, a simple and sensitive methodology based on dynamic headspace solid-phase microextraction (HS-SPME) followed by thermal desorption gas chromatography with quadrupole mass detection (GC-qMSD), was developed and optimized for the determination of volatile (VOCs) and semi-volatile (SVOCs) compounds from different alcoholic beverages: wine, beer and whisky. Key experimental ...

PubMed

32
The Phylogenetic Position of the Pelobiont Mastigamoeba balamuthi Based on Sequences of rDNA and Translation Elongation ...

... extracted with phenol/chloroform/isoamyl alcohol and chloroform/isoamyl alcohol and precipitated with sodium acetate and ethanol (Sambrook, Fritch, and Maniatis 1989). The ... ...

NBII National Biological Information Infrastructure

33
Heterospecific Pairing and Hybridization between Wild Humboldt and Magellanic Penguins in Southern ChileCruzamientos ...

... with phenol, phenol/ chloroform/isoamyl alcohol, and chloroform/isoamyl alcohol, followed by precipitation with 3-M sodium acetate and 100% ethanol. Genomic DNA was resuspended in ... ...

NBII National Biological Information Infrastructure

34
Distribution and altitudinal structuring of phlebotomine sand flies (Diptera: Psychodidae) in southern Anatolia, Turkey:...

... half chloroform isoamyl 23:1), and finally chloroform-isoamyl alcohol (23:1). DNA was precipitated with 1/10 volume 3M sodium acetate and 1 volume isopropanol and stored at �20° ... ...

NBII National Biological Information Infrastructure

35
Metabolomics reveals alterations in both primary and secondary metabolites by wine bacteria.
2009-11-25

Lactic acid bacteria (LAB) were isolated from Korean Meoru (Vitis coigneties) wine and identified as Lactobacillus plantarum meoru0711 (KACC 91436C). The fermentative behavior and metabolic effects of L. plantarum during malolactic fermentation (MLF) were compared with those of the commercial Oenococcus oeni strain through 1H NMR- and GC-based metabolic profiling. Twenty-two primary metabolites of ...

PubMed

36
Glycerol triacetate as solvent and acyl donor in the production of isoamyl acetate with Candida antarctica lipase B.
2009-05-28

Glycerol triacetate was successfully used as a green solvent and as the acyl donor in the transesterification of isoamyl alcohol to produce isoamyl acetate using free and immobilized Candida antarctica lipase B. Immobilized lipase was more catalytically active than free lipase and could be easily separated from the reaction mixture by ...

PubMed

37
Comparative study of thermostability and ester synthesis ability of free and immobilized lipases on cross linked silica gel.
2007-09-20

A novel support has been utilized for immobilization of lipase, which was prepared by amination of silica with ethanolamine followed by cross linking with glutaraldehyde. Lipases from Rhizopus oryzae 3562 and Enterobacter aerogenes were immobilized on activated silica gel, where they retained 60 and 50% of respective original activity. The thermal stability of the immobilized lipases was ...

PubMed

38
DISTRIBUTION OF MOLYBDENUM AND TUNGSTEN IN THE SYSTEM HYDROBROMIC ACID- ORGANIC SOLVENT
1962-01-01

Distribution of Mo and W between hydrobromic acid and ionic acid- containing organic solvents was studied. It was found that with increased acid the extraction of W decreases and the extraction of Mo increases. Extraction of W from 1.0 n hydrobromic solution by given solvents increases accordingly; isoamyl acetate isoamyl ...

Energy Citations Database

39
Increasing the levels of 2-phenylethyl acetate in wine through the use of a mixed culture of Hanseniaspora osmophila and Saccharomyces cerevisiae.
2009-07-30

The impact of mixed cultures of Hanseniaspora osmophila and Saccharomyces cerevisiae with different initial yeast ratios on wine composition has been examined. The mixed culture significantly affected sugar consumption, the main enological parameters and ester concentrations, with the exception of glycerol, isoamyl acetate and diethyl ...

PubMed

40
A Reinvestigation of Brindley�s Gland Exocrine Compounds of Rhodnius prolixus (Hemiptera: Reduviidae)

... isoamyl and amyl alcohols, 2-phenylethanol, and other carboxylic acids and esters. In other triatomine species, no additional analyses of ... ...

NBII National Biological Information Infrastructure

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41
42
UNCLASSIFIED AD NUMBER NEW LIMITATION CHANGE ...
1969-05-01

... gas respirator for protection against organic vapors, will enter an atmosphere containing 0.01 percent of volume (100 ppm)of isoamyl acetate vapor. ...

DTIC Science & Technology

43
Synthesis of a defined antibody library: Design consideration - NASA

Jan 17, 2011 ... The MIT 2006 iGEM team created a system to express methyl salicyate, a mint smelling compound, and isoamyl acetate (banana scent) ...

NASA Website

44
Synthesis of Polyspirocyclobutanes and Related ...
1968-06-01

... move the last traces of isoamyl alcohol from XIVa at 600 and 1-mm pressure, ... mony(III) oxide and calcium acetate dihydrate under nitrogen at 2006C ...

DTIC Science & Technology

45
Studies on the Structure of the Brooding Organs of Two Botryllid Ascidians, Botryllus delicatus and Botryllus sexiens

... the acetone in the specimens was replaced with isoamyl acetate. After being dried at a critical point and ... ...

NBII National Biological Information Infrastructure

46
Specimen Holders for Simultaneous Critical Point Drying of ...
2011-05-14

... The ethanol is then replaced in IO-min steps with increasinL contentrations of isoamyl acetate (50%, 75%, 95%, 100%, 100%). - , Page 5. ...

DTIC Science & Technology

47
Simulant Selection and Environmental Assessment for Open ...
1994-03-01

... nts: Dimethyl SulfoxideL. Triethyl Phosphite. Bis (2-hthylhexyl) Sebacate. Isoamyl Acetate, Dipropyl Sulfane. Sulfur Hexafluoride ...

DTIC Science & Technology

48
Scanning Electron Microscope Study of Ancient Parasite Eggs Recovered From Korean Mummies of the Joseon Dynasty

... were treated in a graded ethanol series and isoamyl acetate, dried in a critical point dryer (Hitachi SCP- ... ...

NBII National Biological Information Infrastructure

49
Role of ets Oncogenes in the Progression of Breast Cancer
1996-10-01

... phe- nol: chloroform: isoamyl alcohol. The DNA was precipi- Materials and methods tated from the aqueous phase with sodium acetate and two ...

DTIC Science & Technology

50
Oscillatory Electric Potential on the Olfactory Epithelium Observed during the Breeding Migration Period in the ...

... by passing air through an aqueous suspension of Isoamyl acetate (50 μl in 50 ml of distilled water) ... ...

NBII National Biological Information Infrastructure

51
Orientation of the Toad, Bufo japonicus, toward the Breeding Pond

... Paralleled by an Increase in Olfactory Sensitivity to Isoamyl Acetate. Chemical Senses 34:8, 667-678Online publication date: ... ...

NBII National Biological Information Infrastructure

52
Occurrence of the Ectoparasite Isocyamus Deltobranchium (Amphipoda: Cyamidae) on Cetaceans from Atlantic Waters

... through a graded ethanol series. After treatment with isoamyl acetate, the specimens were critical-point dried, gold-coated ... ...

NBII National Biological Information Infrastructure

53
Molecular Ecology of Bacterial Populations in Environmental ...
1991-11-30

... time with an equal volume of chloroform/isoamyl alcohol (24:1). The aqueous phase was precipitated with 0.1 volume of 2 M sodium acetate and 2 ...

DTIC Science & Technology

54
In Vitro Reactions of Coelomocytes against Sheep Red Blood Cells in the Solitary Ascidian Halocynthia roretzi

... series and treated for 20 sec with 100% isoamyl acetate. They were quickly transferred to a Hitachi HCP- ... ...

NBII National Biological Information Infrastructure

55
Effect of Micropylar Morphology and Size on Rapid Sperm Entry into the Eggs of the Medaka

... graded series of ethanol solutions. After immersion in isoamyl acetate, they were dried in a critical point apparatus ( ... ...

NBII National Biological Information Infrastructure

56
Description of the Immature Stages of Ophrida xanthospilota (Baly) (Coleoptera: Chrysomelidae: Alticinae) from China

... tetroxide, dehydrated in graded ethanol solution, keep in isoamyl acetate overnight, and critical-point dried in CO2, coated ... ...

NBII National Biological Information Infrastructure

57
Description of the Immature Stages of Bittacus planus Cheng (Mecoptera: Bittacidae) with Notes on Its Biology

... ultimately replaced through two 30 min baths of isoamyl acetate. Eggs were then dried in a CO2 critical- ... ...

NBII National Biological Information Infrastructure

58
Comparison of Cartridge and Membrane Solid-Phase ...
1992-12-01

... extraction of organic compounds from water. could be obtained using isoamyl acetate. Unfortu- Anspach et al. (1982) evaluated 13 commercially ...

DTIC Science & Technology

59
Cold Regions Environmental Test of CB Protective Masks
1989-02-28

... The fit of each mask will be evaluated by exposing the participants te isoamyl acetate (banana oil) fumes after the mask has been donned. ...

DTIC Science & Technology

60
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61
Cell Body Contraction of Splrostomum Does Not Involve Shortening of Inter-Kinetosomal Distance along Ciliary Lines

... dehydrated through a graded ethanol series, transferred to isoamyl acetate and finally dried by using a critical point ... ...

NBII National Biological Information Infrastructure

62
Attachment and Penetration of Centrocestus armatus (Digenea: Heterophyidae) Cercariae to Gills of Secondary ...

... graded series of ethanol, and then exchanged with isoamyl acetate. The specimens were critical-point dried (Hitachi HCP- ... ...

NBII National Biological Information Infrastructure

63
Annotated Bibliography for Gas Mask and Chemical Defense ...
1988-01-15

... Page 12. Three qualitative respirator fit tests: the negative pressure test, the isoamyl acetate test, aad the irritant smoke test were performed concur-- ...

DTIC Science & Technology

64
A Potential Field Expedient Test for Face Mask Integrity
1987-10-01

... the order of 3000 which represents a potentially significant improvement over the protection factor provided by isoamyl acetate (banana oil), which ...

DTIC Science & Technology

65
A NEW SPECIES OF MACROBOTHRIIDAE (CESTODA: DIPHYLLIDEA) FROM THORNBACK RAY PLATYRHINA SINENSIS IN CHINA

... water solution, dehydrated in ethanol series, replaced in isoamyl acetate, dried through the CO2 critical point, and viewed ... ...

NBII National Biological Information Infrastructure

66
Purification and properties of Amycolatopsis mediterranei DSM 43304 lipase and its potential in flavour ester synthesis.
2010-11-21

An extracellular thermostable lipase from Amycolatopsis mediterranei DSM 43304 has been purified to homogeneity using ammonium sulphate precipitation followed by anion exchange chromatography and hydrophobic interaction chromatography. This protocol resulted in a 398-fold purification with 36% final recovery. The purified A. mediterranei DSM 43304 lipase (AML) has an apparent molecular mass of 33 ...

PubMed

67
42 CFR 84.205 - Facepiece test; minimum requirements.
2010-10-01

...wearer will enter a chamber containing 100 p.p.m. isoamyl acetate vapor for half-mask facepieces, and 1,000 p... (4) Each wearer shall not detect the odor of isoamyl-acetate vapor during the...

Code of Federal Regulations, 2010

68
Genetic and physiological analysis of branched-chain alcohols and isoamyl acetate production in Saccharomyces cerevisiae.
2002-06-22

Branched-chain alcohols, such as isoamyl alcohol and isobutanol, and isoamyl acetate are important flavor components of yeast-fermented alcoholic beverages. Analysis of a null mutant of the BAT2 gene encoding cytosolic branched-chain amino acid aminotransferase, and a transformant with multi-copy plasmids containing the BAT2 gene ...

PubMed

69
Direct conversion of acetals to esters with high regioselectivity via O,P-acetals.
2011-06-29

A new direct conversion of O,O-acetals to esters via O,P-acetal intermediates was developed. The regioselective cleavage of unsymmetrical cyclic acetals occurred to give the more crowded esters as single isomers. PMID:21713294

PubMed

70
NIOSH-OSHA Draft Technical Standards for Set D, Part II.
1975-01-01

Draft technical standards are outlined for sec-hexyl acetate, isoamyl acetate, isobutyl acetate, methyl acetate, methyl acrylate, methyl cellosolve acetate, methyl methacrylate and n-propyl acetate. Each standard includes definitions, monitoring ...

National Technical Information Service (NTIS)

71
Acetone, methyl ethyl ketone, ethyl acetate, acetonitrile and other polar aprotic solvents are strong inducers of aneuploidy in Saccharomyces cerevisiae.
1985-05-01

A diploid yeast strain D61.M was used to study induction of mitotic chromosomal malsegregation, mitotic recombination and point mutation. Several ketones (including acetone and methyl ethyl ketone) and some organic acid esters (including the methyl, ethyl and 2-methoxyethyl esters of acetic acid) and acetonitrile strongly induced ...

PubMed

72
Water-Displacing Fluids and Their Application to ...
1948-10-04

... Methyl hexyl carbinol 26.5 dynes/cm Isoamyl alcohol 24.1 11 If Tert ... acetone , amyl acetate , cel losolve Acetate , ethyl acetoacetate , butyl or piropyl ...

DTIC Science & Technology

73
PREPARATION OF METALLIC URANIUM
1958-01-01

please delete the no. 721012:007211Complex saits of uranyl nitrate with diethyl ether, ethyl acetate, acetone, nitromethane, and methyl alcohol were obtained, as well as the uranyl acetate wdth methyl, ethyl, and isoamyl alcohol in the crystalline lattice. Their luminescence spectra were studied. (tr-auth)

Energy Citations Database

74
Enhanced production of isoamyl alcohol and isoamyl acetate by ubiquitination-deficient Saccharomyces cerevisiae mutants.
2005-01-01

Aromatic compounds are an important element in the flavor of yeast-fermented alcohol. We isolated mutants of Saccharomyces cerevisiae capable of growth at high levels of hydrostatic pressure. Among them, the HPG1 mutants, with a defect in their Rsp5 ubiquitin ligase, were found to produce high amounts of aromatics due to enhanced leucine uptake, with isoamyl alcohol production ...

PubMed

75
Rapid Transacylations of Activated Ester Substrates Bound to ...
1990-02-20

... HYDROLYSIS, *ESTERS, ACCELERATION, ENZYMES, SUBSTRATES, CATALYSIS, PHENOLS, HYDROXIDES, ACETATES, HYDROXYL ...

DTIC Science & Technology

76
The Saccharomyces cerevisiae alcohol acetyl transferase Atf1p is localized in lipid particles.
2004-03-01

The yeast alcohol acetyl transferase I, Atf1p, is responsible for the major part of volatile acetate ester production in fermenting Saccharomyces cerevisiae cells. Some of these esters, such as ethyl acetate and isoamyl acetate, are important for the fruity flavours of ...

PubMed

77
Heavy sulphur compounds, higher alcohols and esters production profile of Hanseniaspora uvarum and Hanseniaspora guilliermondii grown as pure and mixed cultures in grape must.
2008-03-31

Hanseniaspora guilliermondii and Hanseniaspora uvarum were tested in grape must fermentations as pure and mixed starter cultures with Saccharomyces cerevisiae. In pure cultures, the specific growth rates found were 0.29 h(-1) for H. uvarum, 0.23 h(-1) for H. guilliermondii and 0.18 h(-1) for S. cerevisiae. No significant differences were observed between these values and those obtained in mixed ...

PubMed

78
Preliminary Studies on The Crosslinking of Poly(ester-acetals) ...
1966-03-23

... Title : Preliminary Studies on The Crosslinking of Poly(ester-acetals) and Poly(amide-acetals) on Glass Substrates,. Corporate Author : ...

DTIC Science & Technology

79
Nitrogen addition influences formation of aroma compounds, volatile acidity and ethanol in nitrogen deficient media fermented by Saccharomyces cerevisiae wine strains.
2009-08-01

The effects of nitrogen addition into nitrogen deficient/depleted media on the release of aroma compounds post-fermentation were investigated in three commercial yeast strains of Saccharomyces cerevisiae which highlight the yeast strain effect as well as nitrogen effects. By comparing the two timings of nitrogen addition, prior to fermentation or later at stationary phase (72 h), it was shown that ...

PubMed

80
Effect of fungicide residues on the aromatic composition of white wine inoculated with three Saccharomyces cerevisiae strains.
2004-03-10

The effects of three fungicide residues (cyprodinil, fludioxonil, and pyrimethanil) on the aromatic composition (acids, alcohols, and esters) of Vitis vinifera white wines (var. Air�n) inoculated with three Saccharomyces cerevisiae strains (syn. bayanus, cerevisiae, and syn. uvarum) are studied. The aromatic exponents were extracted and concentrated by adsorption-thermal ...

PubMed

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81
Biochemical pathways generating post-mortem volatile compounds co-detected during forensic ethanol analyses.
2007-04-23

In this contribution are presented the fermentations of the main substrates present in a decaying corpse, namely carbohydrates, amino acids, glycerol and fatty acids, generating the post-mortem volatile compounds that could be detected along with ethanol during the forensic ethanol analysis. The available literature (preferably reviews) on microbial metabolic pathways (enzymes, substrates, ...

PubMed

82
A comparison of laboratory and pilot-scale fermentations in winemaking conditions.
2010-05-12

We investigated the influence of the fermenter size on alcoholic fermentation. Experiments were carried out at pilot scale, in 100-L fermenters, and at laboratory scale, in stirred and static 1-L fermenters. Two musts, Grenache blanc and Sauvignon, were fermented with and without the addition of solid particles from grape musts. Highly clarified must fermentation kinetics was strongly affected by ...

PubMed

83
Controlled formation of volatile components in cider making using a combination of Saccharomyces cerevisiae and Hanseniaspora valbyensis yeast species.
2005-11-15

The effect of pure and mixed fermentation by Saccharomyces cerevisiae and Hanseniaspora valbyensis on the formation of major volatile components in cider was investigated. When the interaction between yeast strains of S. cerevisiae and H. valbyensis was studied, it was found that the two strains each affected the cell growth of the other upon inoculation of S. cerevisiae during growth of H. ...

PubMed

84
Influence of volatile compounds produced by yeasts predominant during processing of Coffea arabica in East Africa on growth and ochratoxin A (OTA) production by Aspergillus ochraceus.
2005-10-30

The effects of volatile compounds produced during coffee processing by Pichia anomala, P. kluyveri and Hanseniaspora uvarum on growth of Aspergillus ochraceus and production of ochratoxin A (OTA) were studied. On malt extract agar (MEA) and on coffee agar (CA), exposure of A. ochraceus to the gaseous phase of malt yeast glucose peptone (MYGP) plates inoculated with P. anomala, P. kluyveri and H. ...

PubMed

85
RELATEDNESS WITHIN NEST GROUPS OF THE SOUTHERN FLYING SQUIRREL USING MICROSATELLITE AND DISCRIMINANT FUNCTION ANALYSES

... phenol�:�chloroform/isoamyl alcohol (24:1), and chloroform�:�isoamyl alcohol (24:1). DNA was precipitated by using 0.2 volumes of 10 M ammonium acetate and 1 volume of 95% ethanol. DNA yield ... ...

NBII National Biological Information Infrastructure

86
DISTRIBUTION OF Li ISOTOPES IN IMMISCIBLE SOLVENTS
1962-11-01

Distribution coefficients of Li isotopes equilibrated between the lithium salts in aqueous solutions and Li salts in acetone, isoamyl, alcohol, diethyl ether, and amyl acetate were determined. Additions of a light petroleum fraction to lithium chloride solution in mixed methylamine and isoamyl alcohol in contact with aqueous ...

Energy Citations Database

87
Release of isoamyl acetate from starch pastes of various structures: thermodynamic and kinetic parameters.
2004-08-25

The release of isoamyl acetate from starch-based matrices was studied on the basis of a cumulative dynamic headspace analysis. Two corn starches were compared, which are known to yield pastes and gels differing in their structures and properties. These properties were assessed by viscometry and viscoelastic measurements. Aroma release was discussed as a ...

PubMed

88
A kinetic study of isoamyl acetate synthesis by immobilized lipase-catalyzed acetylation in n-hexane.
2006-07-16

The objective of this work was to propose a reaction mechanism and to develop a rate equation for the synthesis of isoamyl acetate by acylation of the corresponding alcohol with acetic anhydride using the lipase Novozym 435 in n-hexane. The reaction between isoamyl alcohol and acetic anhydride ...

PubMed

89
Evaluation of 2-Cyanoacrylate Ester Monomers as a Quick-Setting Adhesive for Wet Dialysis.
1969-01-01

Ethyl, propyl and isopropyl 2-cyanoacrylate monomers appear to be a possible candidate as quick-setting adhesives for the adhesion of wet regnerated cellulose dialysis membranes. Of these, isopropyl or isoamyl 2-cyanoacrylate seem to warrant further inves...

National Technical Information Service (NTIS)

90
The Basic Components of the Cuckoopint (Arum maculatum L. ...
1970-06-26

... y ) Impregnation according to Steiner et al: With 0.15 m sodium acetate solution (5 min.). ... 1 0 Ethanol (1) (h Rf values of the DNB of Isoamyl-41 and ...

DTIC Science & Technology

91
Testicular Regression in Response to Food Restriction and Short Photoperiod in White-Footed Mice (Peromyscus leucopus) ...

... then lightly homogenized and extracted with phenol/chloroform/isoamyl alcohol. After precipitation with sodium acetate and isopropanol, total DNA was quantified spectophotometrically based ... ...

NBII National Biological Information Infrastructure

92
Telomere Length in Male Germ Cells Is Inversely Correlated with Telomerase Activity1

... The digest was deproteinized by successive phenol/chloroform/isoamyl alcohol extractions and recovered by precipitation with 100% ethanol in 7.5 M ammonium acetate. The DNA was dissolved in Tris-EDTA buffe...

NBII National Biological Information Infrastructure

93
Small-Scale Isolation of High Molecular Weight DNA from Leishmania braziliensis

... min. The extraction is repeated with phenol/chloroform/isoamyl alcohol and the DNA precipitated by adding 0.1 volumes of 3 M sodium acetate, pH 5.3, and 2 volumes of absolute ... ...

NBII National Biological Information Infrastructure

94
POLYMER STRUCTURES AND PROPERTIES
1961-04-30

... n-hexane; isoamyl alcohol, n-amyl alcohol, sec-butanol, 2-heptanol, n-butyl carbinol; ethyl lactate, butyl cellosolve, ethyl formate, isopropyl acetate, ...

DTIC Science & Technology

95
NIOSH-OSHA Draft Technical Standards for Set E, Part II.
1976-01-01

Draft technical standards are outlined for hydroquinone, isoamyl alcohol, isobutyl alcohol, isopropyl alcohol, isopropyl acetate, methyl alcohol, methyl isobutyl carbinol, and propyl alcohol. Each standard includes definitions, monitoring requirements, me...

National Technical Information Service (NTIS)

96
Mechanism of Interaction of Radiowaves and Microwaves with ...
1990-03-01

... 25:24:1), and once with chloroform:isoamyl alcohol (25:1). Plasmid DNA was precipitated by addition of 0.1 volume of 3 M sodium acetate pH 6.5 ...

DTIC Science & Technology

97
Mechanism of Interaction of Deoxyribonucleic Acid (DNA) with ...
1990-03-01

... 25:24:1) and once with chloroform:isoamyl alcohol (25:1). Plasmid DNA was precipitated by adding 0.1 volume of 3M sodium acetate pH 6.5 and 2 ...

DTIC Science & Technology

98
Isolating a Cell Maximally Secreting Acetylcholinesterase
1986-07-07

... Since the 14C-Acetate is organic, it partitions into the organic ... 400 ml isoamyl alcohol (Mallinckrodt #2992070) 17.2g PPO (Amersham #190020) 1.0 ...

DTIC Science & Technology

99
I11111 111111ll Ill11 Ill11 IIIII IIIII 11111 11111 Ill11 IIIII ...

Acetic and propionic acid and. Prevotella. Bac 5. Isovaleric acid. 65 isoamyl alcohol ... isovaleric and isocaproic acid. Isovaleric acid. *This is a fungus ...

NASA Website

100
Flame Temperatures and Internal Pressures of Pyrotechnic ...
1982-03-01

... '0.8 1.5 0.8 50 ms (a) 76.5% Lead Mononitrorescorcinate 8.5% Potassium Chlorate 15.0% 1/2 Sec. Nitrocotton, in Iso-Amyl Acetate ...

DTIC Science & Technology

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101
Evidence from Genetic Markers Suggests Seasonal Variation in Dispersal in Heliothis virescens (Lepidoptera: Noctuidae)

... mixed with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 PCI). The homogenate was cooled, and 30 μl of 8 M potassium acetate was added to precipitate the protein. After incubation ... ...

NBII National Biological Information Infrastructure

102
DNA fingerprinting using AFLP markers to search for markers associated with yield attributes in the silkworm, Bombyx ...

... at 14,000 rpm. 10 μl of potassium acetate (8M) were added to the extract before centrifugation. The aqueous phase was recovered and re-extracted with an equal volume of a chlorophorm- isoamyl alcohol mixtu...

NBII National Biological Information Infrastructure

103
Complex Evolution of Bile Salts in Birds�volution complexe des sels biliaires chez les oiseaux

... two solvent systems: (1) isoamyl acetate:propionic acid:1-propanol:water 4:3:2:1 (v/v) (Hofmann 1962); and (2) a double ... ...

NBII National Biological Information Infrastructure

104
Attempted Synthesis of 1,4-Dinitro(3,4-b)-(3,4-e) ...
1989-07-01

... The anion would then be treated with Isoamyl nitrite to form the c ... showed no ovidanco of deburizyistion using Pd/C In ethyl acetate, methanol, or ...

DTIC Science & Technology

105
A Rapid Method for Detection and Identification of Flaviviruses ...
2011-05-14

... Sodium acetate, pH 4.0, was added to yield a final concentra- t ... of water saturated phenol and one fifth volume of chloroform:isoamyl alcohol (49:1 ...

DTIC Science & Technology

106
42 CFR 84.104 - Gas tightness test; minimum requirements.
2010-10-01

... (a) Each apparatus will be tested for tightness by persons wearing it in an atmosphere of 1,000 p.p.m. isoamyl acetate. (b) Six persons will each wear the apparatus in the test concentrations specified in paragraph (a) of...

Code of Federal Regulations, 2010

107
Cellulose Nitrate-Acetate Mixed Esters.
1964-01-01

A preliminary investigation of the preparation and properties of cellulose nitrate-acetate mixed esters was conducted. These mixed esters can be prepared in a wide range of amounts of substitution of both acetate and nitrate groups either through acetylat...

National Technical Information Service (NTIS)

108
Volatile compounds of young wines from cabernet sauvignon, cabernet gernischet and chardonnay varieties grown in the loess plateau region of china.
2010-12-10

In order to elucidate the aroma components of wine produced in the Loess Plateau region of China, volatile compounds of young wines from Cabernet Sauvignon, Cabernet Gernischet and Chardonnay varieties grown in the new ecological region were investigated for the first time in this research. Among the volatile compounds analyzed by HS-SPME with GC-MS, a total of 45, 44 and 42 volatile compounds ...

PubMed

109
The Mercuration of Phosphonate Esters.
1973-01-01

The reactions of several ethyl phosphonate esters with mercuric acetate gave the corresponding bis(acetoxymercuri) phosphonates. Various reactions of these compounds are reported.

National Technical Information Service (NTIS)

110
Nitrated and/or Fluorinated Ethyl Amino Acetic Acids and ...
1974-10-29

... Title : Nitrated and/or Fluorinated Ethyl Amino Acetic Acids and Esters ... Abstract : This invention is to provide carboxylic acid esters that can be ...

DTIC Science & Technology

111
CELLULOSE NITRATE-ACETATE MIXED ESTERS.
1964-06-01

... mixed esters can be prepared in a wide range of amounts of substitution of both acetate and nitrate groups either through acetylation of cellulose ...

DTIC Science & Technology

112
Azelaaldehydic Acid Ester-Acetal Derivatives as Plasticizers ...
1963-09-08

... Accession Number : ADD427366. Title : Azelaaldehydic Acid Ester-Acetal Derivatives as Plasticizers for Poly(Vinyl Chloride). ...

DTIC Science & Technology

113
Esters Dissociatively Adsorb on Alumina.
1983-02-01

... For ethyl-d5 acetate, the spectrum is distinguishable from the spectrum of a symmetrically bonded acetate ion. (Author). ...

DTIC Science & Technology

114
Method of Obtaining Difluoroaminoacetic Acid Esters.
1974-01-01

The Russian patent concerns a method for obtaining esters of difluoroaminoacetic acid. It is distinguished by the fact that the ester of acetic acid is treated with tetrafluorohydrazine in a medium of an inert solvent with heating. The process is carried ...

National Technical Information Service (NTIS)

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