... of acetylcarnitine from acetyl coenzyme A (CoA ... ACIDS, OXIDATION), (*MUSCLES, ENZYMES), TRANSFERASES, COENZYMES, DIPTERA, FLIGHT ...
DTIC Science & Technology
The essential oil from mature leaves of flowering peppermint (Mentha piperita L.) contains up to 15% (�)-menthyl acetate, and leaf discs converted exogenous (�)-[G-3H]menthol into this ester in approximately 15% yield of the incorporated precursor. Leaf extracts catalyzed the acetyl coenzyme A-dependent ...
PubMed Central
It is demonstrated that there are two pathways from pyruvate to acetyl coenzyme A in yeast cells. Maceration juice contains the enzymes required for the first pathway: pyruvate decarboxylase, acetaldehyde dehydrogenase and aceto-CoA-kinase, the coenzyme r...
National Technical Information Service (NTIS)
Two pathways from pyruvate to acetyl-coenzyme A in yeast. Online Source: Click to View ... and flavine (FMN, FAD) coenzymes from the media of incubation. ...
NASA Website
2. Studies on acetyl-coenzyme A synthetase of yeast - Inhibition by long-chain acyl-coenzyme A esters. Author: Satyanarayana, T.; Klein, H. P. ...
... to flufenacet/metribuzin. Cross-resistance to all acetyl coenzyme A carboxylase-inhibiting (group 1) herbicides was observed ... the region, and are typically controlled with acetyl coenzyme A carboxylase...
NBII National Biological Information Infrastructure
... words: Resistance test, aryloxyphenoxypropanoate, phenylurea. Abbreviations: ACCase, acetyl coenzyme A carboxylase (EC 6.4.1.2); APP, ... al. 1993; Saari et al. 1994) or acetyl coenzyme A carboxylase (ACC...
... level of Japanese foxtail to haloxyfop, an acetyl coenzyme A carboxylase (ACCase; EC 6.4.1.2)� ... D. L. Wyse. 1987. Inhibition of plant acetyl coenzyme A carboxylase by the herbicides sethoxydim and halox...
A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives thereof which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides.
Energy Citations Database
A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives are disclosed which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides. 5 figs.
DOEpatents
... index words: Hybridization, outcrossing, SORHA. Abbreviations: ACCase, acetyl coenzyme-A carboxylase (EC 6.4.1.2); AOPP, ... often reduced sensitivity of the target enzyme, acetyl coenzyme-A carboxylase (...
... differed slightly in the specific activity of acetyl�coenzyme A carboxylase (ACCase) (EC 6.4.1.2) ... compounds are potent inhibitors of the enzyme acetyl�coenzyme A (CoA) carboxylase (ACCase) (Gronwald 1...
... Marshall. 1995. Target enzyme-based resistance to acetyl-coenzyme A carboxylase inhibitors in Eleusine indica. Pestic. Biochem. ... Coble. 1998. Herbicide inhibition and characterization of acetyl-coenzym...
The terminal steps in the biosynthesis of the monoterpenoid indole alkaloids vindoline and minovincinine are catalyzed by separate acetyl coenzyme A-dependent O-acetyltransferases in Madagascar periwinkle (Catharanthus roseus G. Don). Two genes were isolated that had 63% nucleic acid identity and whose deduced amino acid sequences ...
Cell extracts of butyrate-forming clostridia have been shown to catalyze acetyl-coenzyme A (acetyl-CoA)- and ferredoxin-dependent formation of H2 from NADH. It has been proposed that these bacteria contain an NADH:ferredoxin oxidoreductase which is allosterically regulated by acetyl-CoA. We report here that ferredoxin reduction with ...
PubMed
Cell extracts of butyrate-forming clostridia have been shown to catalyze acetyl-coenzyme A (acetyl-CoA)- and ferredoxin-dependent formation of H2 from NADH. It has been proposed that these bacteria contain an NADH:ferredoxin oxidoreductase which is allosterically regulated by acetyl-CoA. We report here that ...
Mar 1, 2011 ... Effects of aeration on formation and localization of the acetyl coenzyme A synthetases of Saccharomyces cerevisiae ...
BS>A study was made of the acetylating function of the coenzyme A system and of the changes in the concentration of the coenzyme in the liver of a pigeon in the case of acute radiation disease caused by a single dose of 2,000 to 3,000 r x radiation. The acetylating activity was measured in the intact ...
Metabolism This collection curated by Stephen Chanock (sc83a@nih.gov) Gene Description Sequences Sequence assembly Predicted SNPs having score >= 0.99 ACAT1 Acetyl-Coenzyme A acetyltransferase 1 (acetoacetyl Coenzyme A thiolase) D90228 D10511 ACAT2 Acetyl-Coenzyme
Cancer.gov
Updated/Added to NTRS: Dec 04, 2004. 2. Effects of aeration on formation and localization of the acetyl coenzyme A synthetases of Saccharomyces cerevisiae ...
... inactive products. APPs are applied as relatively inactive carboxylic acid esters (e.g., fenoxaprop-ethyl, diclofop-methyl) to enhance ... ...
In polyacrylamide gel electrophoresis in .the presence of sodium dodecyl sulfate , the molecular ..... Polyacrylamide gel electrophoresis of aerobic ACS. ...
... herbicide resistance, ACCase inhibitors, graminicides. Abbreviations: ACCase, acetyl coenzyme-A carboxylase; APP, aryloxyphenoxypropionate; CHD, cyclohexanedione; DAT, days ... ...
... pathway, the evolution of acetyl-coenzyme A carboxylase, cell wall physiology, the functions of microtubules and the cell ... the hormone system; and the cell cycle and cell wall formation. The specific n...
... resistance, postemergence grass herbicides, turfgrass. Abbreviations: ACCase, acetyl-coenzyme A carboxylase; APP, aryloxyphenoxypropionate; CHD, cyclohexanedione; smooth crabgrass ( ... ...
... ACCase inhibitors, herbicide resistance, graminicides. Abbreviations: ACCase, acetyl coenzyme-A carboxylase; WAT, weeks after treatment. INTRODUCTION Johnsongrass ... ...
... words: ACCase inhibitors, herbicide resistance. Abbreviations: ACCase, acetyl coenzyme-A carboxylase; AOPP, aryloxyphenoxypropionate; CHD, cyclohexanedione. Introduction The ... ...
Mar 1, 2011 ... Similarly, an acetyl-CoA synthetase associated predominantly with anaerobic ... were three mitochondrial enzymes that attained maximal activity only under ... Abstract: Acetyl-coenzyme A synthetase in aerobic yeast cells ...
The enolization of acetyl coenzyme A is demonstrated by means of isotope experiments. It requires citrate-synthase and an inductor, (S)-malate. The mechanism of the biosynthesis of citrate and the results of the experiments are in agreement with the sugge...
... cross-resistance to cycloxydim, propaquizafop, and fluazifop (acetyl coenzyme A [CoA] carboxylase [ACCase]-inhibiting herbicides), and multiple ... 318. BioOneDélye, C. 2005. Weed resistance to acetyl coe...
The synthesis of high specific radioactivity (/sup 14/C)-acetyl-Coenzyme A from (/sup 14/C)sodium acetate, 2,6-dichlorobenzoic acid, 1,1'-carbonyldiimidazole, and CoA is reported. Starting with 1 mumol of (/sup 14/C)sodium acetate, this method yields pure (/sup 14/C)acetyl-CoA in yields approaching 40%. Chromatography on a reversed-phase ODS ...
with phosphate-buffered saline (PBS), and lysed by sonication. Determination of extracellular acetate March 1995 Acetyl coenzyme A synthetase (Acs) activates acetate to acetyl coenzyme A through an acetyladenylate intermediate; two other enzymes, acetate kinase (Ack) and phosphotransacetylase (Pta), activate
E-print Network
Benzene, toluene, ethylbenzene, and xylenes (BTEX compounds) are primary contimantes of concern in aquifers and sediments where petroleum leakages and spills have occured. This paper describes anaerobic toluene metabolism by a new bacterium, which was isolated from petroleum contaminated aquifer sediment. Also provided is evidence for important modifications in the acetylCoA ...
Escherichia coli strains overproducing the response regulator CheY respond to acetate by increasing their clockwise bias of flagellar rotation, even when they lack other chemotaxis proteins. With acetate metabolism mutants, we demonstrate that both acetate kinase and acetyl coenzyme A synthetase are involved in this response. Thus, a response was observed ...
... 889. BioOneDelye, C. 2005. Weed resistance to acetyl coenzyme A carboxylase inhibitors: an update. Weed Sci 53: ... Marshall. 1995. Target enzyme-based resistance to acetyl-coenzyme-A carboxylase inhibitor...
In vivo expression of CO dehydrogenase/acetyl coenzyme A synthase in Methanosarcina spp. is coordinately regulated in response to substrate by at least two mechanisms: differential transcription initiation and early elongation termination near the 3? end of a 371-bp leader sequence. This is the first report of regulation of transcription elongation in the ...
1. A radiochemical method for the estimation of choline acetyltransferase (choline acetylase) has been devised which involves the formation of labelled acetylcholine from labelled acetate. 2. [1-(14)C]Acetate and coenzyme A are pre-incubated in the presence of non-rate-limiting concentrations of acetyl-coenzyme A synthetase to give ...
Many microorganisms produce surface-active substances that enhance the availability of water-insoluble substrates. Although many of these biosurfactants have interesting potential applications, very little is known about their biosynthesis. The basidiomycetous fungus Ustilago maydis secretes large amounts of mannosylerythritol lipids (MELs) under conditions of nitrogen starvation. We recently ...
Phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Azotobacter vinelandii, like the corresponding enzyme from other organisms, is activated by acetyl coenzyme A and inhibited by l-aspartate. Both modifiers affect primarily the affinity of the enzyme for phosphoenolpyruvate. This is the first enzyme with a strictly anaplerotic (intermediate-replacing) ...
The carbon monoxide dehydrogenase (CODH) complex from Methanosarcina thermophila catalyzed the synthesis of acetyl coenzyme A (acetyl-CoA) from CH3I, CO, and coenzyme A (CoA) at a rate of 65 nmol/min/mg at 55 degrees C. The reaction ended after 5 min with the synthesis of 52 nmol of acetyl-CoA ...
It was shown that, 6 to 7 days after irradiation of pigeons in doses 2 to 3 thousand r, the intensity of acetylation processes in liver homogenates fell from a mean normal value of 465 plus or minus 16 mu g sulfanilamide per g fresh tissue in 20 min to a corresponding value of 119 plus or minus 8.5 mu g. Inhibition of acetylation has also been ...
Cell-free extracts of Treponema pallidum catalyzed the decarboxylation of pyruvate. This activity was suppressed at low O2 tensions and appeared to be coenzyme A independent. Pyruvate decarboxylation was inorganic phosphate dependent, and evidence suggested that acetyl phosphate was a product. Oxygen was consumed, and data indicated that H2O2 was produced. ...
Acetyl-coenzyme A (CoA) carboxylase catalyzes the first step in the biosynthesis of fatty acids in bacteria and eukaryota. This enzyme is the target of drug design for treatment of human metabolic diseases and of herbicides acting specifically on the eukaryotic form of the enzyme in grasses. Acetyl-CoA carboxylase activity screening in drug and herbicide ...
... Abstract Acetyl-coenzyme A carboxylase (ACCase) assays and absorption, translocation, and metabolism experiments were conducted to investigate ... fluazifop-P. The rate of [14C]quizalofop-ethyl absorption...
... of ACACA (acetyl-Coenzyme A carboxylase alpha). The adenovirus-mediated production of dominant-negative PRKAA totally abolished ... PRKAA Thr172 phosphorylation with the dominant-negative PRKAA adenovirus...
The use of (14C)acetate to label the phospholipids of stringent Escherichia coli, after a decrease in agitation, leads to artifacts resulting from a decrease in the specific activity of the acetyl coenzyme A pool.
... 2000), acetolactate synthase inhibitor resistance in black nightshade (Solanum nigrum L.) (Volenberg and Stoltenberg 2002b), and acetyl-coenzyme ... 2002b. Inheritance of resistance in eastern black night...
... reported for the facultatively cross-pollinating species kochia [Kochia scoparia (L.) Schrad.] (Stallings et al. 1995) and for ... Pollen-mediated gene flow of sulfonylurea-resistant kochia (Kochia scopar...
Dideoxykanamycin B was inactivated by an enzyme isolated from P. aeruginosa cape 15 in the presence of ATP, magnesium acetate and coenzyme A. This inactivation did not take place without either ATP or magnesium ion but the activity was restored by the add...
... July 12, 2006; Accepted: October 31, 2006Keywords: Acetyl-coenzyme A carboxylase, herbicide resistance, lipid biosynthesis inhibitor, graminicide ... downy brome population with possible resistance to ace...
A series of binuclear NiNi complexes supported by a single thiolate bridge and containing a methylnickel moiety have been prepared and fully characterized. The complexes represent structural analogues for the proposed organonickel intermediate in the acetyl coenzyme A synthase catalytic cycle. Variable temperature 31P NMR spectroscopy was used to examine ...
Acetyl coenzyme A (acetyl-CoA) is a central metabolite in carbon and energy metabolism. Because of its amphiphilic nature and bulkiness, acetyl-CoA cannot readily traverse biological membranes. In fungi, two systems for acetyl unit transport have been identified: a shuttle dependent on the ...
The purified nickel-containing CO dehydrogenase complex isolated from methanogenic Methanosarcina thermophila grown on acetate is able to catalyze the exchange of [1-14C] acetyl-coenzyme A (CoA) (carbonyl group) with 12CO as well as the exchange of [3'-32P]CoA with acetyl-CoA. Kinetic parameters for the carbonyl exchange have been determined: Km ...
Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate ...
..cap alpha..,..beta..-Unsaturated coenzyme A(CoA) thioesters including acrylyl CoA, methacrylyl CoA, and propiolyl CoA were synthesized by catalysis with acetyl CoA synthetase. After isolation from the enzymatic reactions, the products were found to be the result of 1,4 addition of CoASH to the double bond and addition of water to the triple bond of the ...
Carbon monoxide dehydrogenase catalyzes the synthesis of acetyl-coenzyme A from coenzyme A, a methyl group, and carbon monoxide. The carbon monoxide binds to a mixed metal center of the enzyme, which contains nickel bridged to an iron-sulfur cluster. Resonance Raman spectroscopy has been used to identify both C-O stretching and metal-CO stretching ...
NASA Astrophysics Data System (ADS)
In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs). The acs gene, coding for the single Acs subunit, was isolated from a genomic library of M. soehngenii DNA in Escherichia coli by using antiserum raised against the purified Acs. ...
1. A radiochemical method for the estimation of choline acetyltransferase (choline acetylase) has been devised which involves the formation of labelled acetylcholine from labelled acetate. 2. [1-14C]Acetate and coenzyme A are pre-incubated in the presence of non-rate-limiting concentrations of acetyl-coenzyme A synthetase to give ...
A rapid procedure is described for assaying chloramphenicol acetyltranserase (CAT) enzyme activity following transfection of the CAT gene into eukaryotic cells. CAT enzyme activity in cell extracts catalyzes the transfer of (/sup 14/C)acetyl groups from labeled acetyl coenzyme A to unlabeled chloramphenicol. Labeled reaction product is ...
In the aerobic metabolism of aromatic substrates, oxygenases use molecular oxygen to hydroxylate and finally cleave the aromatic ring. In the case of the common intermediate benzoate, the ring cleavage substrates are either catechol (in bacteria) or 3,4-dihydroxybenzoate (protocatechuate, mainly in fungi). We have shown before that many bacteria, e.g. Azoarcus evansii, the organism studied here, ...
A novel procedure for the quantitative isolation and purification of acyl-coenzyme A esters is presented. The procedure involves two steps: 1) tissue extraction using acetonitrile/2-propanol (3+1, v+v) followed by 0.1M potassium phosphate, pH 6.7, and 2) purification using 2-(2-pyridyl)ethyl functionalized silica gel. Recoveries determined by adding radiolabelled ...
Acetyl coenzyme A (CoA) is a central metabolite in carbon and energy metabolism and in the biosynthesis of cellular molecules. A source of cytoplasmic acetyl-CoA is essential for the production of fatty acids and sterols and for protein acetylation, including histone acetylation in the nucleus. ...
On the basis of radioisotope-incorporation experiments it is suggested that acetyl-CoA, an obligatory intermediate in chloroplast terpenoid biosynthesis, may be formed in maize from photosynthetically fixed carbon dioxide by the route carbon dioxide?glycollate?glyoxylate?glycine?serine?pyruvate?acetyl-CoA. The proposed route is supported by conventional ...
The pyruvate dehydrogenase component (E1) of the pyruvate dehydrogenase complex catalyzes the decomposition of 3-fluoropyruvate to CO/sub 2/, fluoride anion, and acetate. Acetylthiamin pyrophosphate (acetyl-TPP) is an intermediate in this reaction. Incubation of the pyruvate dehydrogenase complex with 3-fluoro(1,2-/sup 14/)pyruvate, TPP, coenzyme A ...
Coenzyme A (CoA) and its thioester derivatives are important precursor molecules for many industrially useful compounds such as esters, PHBs, lycopene and polyketides. Previously, in our lab we could increase the intracellular levels of CoA and acetyl-Coenzyme A (acetyl-CoA) by overexpressing one of the upstream rate-controlling ...
S-Trifluoroacetonyl-coenzyme A has been synthesized in 87% yield by reaction of 1,1,1-trifluoro-3-bromopropanone with trilithium coenzyme A in presence of pyridine. The compound was characterized by its ultraviolet absorption spectrum and 1H and 19F nuclear magnetic resonance spectra. The alpha-methylene protons of the S-trifluoroacetonyl group exchanged ...
