... index words: Hybridization, outcrossing, SORHA. Abbreviations: ACCase, acetyl coenzyme-A carboxylase (EC 6.4.1.2); AOPP, ... often reduced sensitivity of the target enzyme, acetyl coenzyme-A carboxylase (...
NBII National Biological Information Infrastructure
... herbicide resistance, ACCase inhibitors, graminicides. Abbreviations: ACCase, acetyl coenzyme-A carboxylase; APP, aryloxyphenoxypropionate; CHD, cyclohexanedione; DAT, days ... ...
... ACCase inhibitors, herbicide resistance, graminicides. Abbreviations: ACCase, acetyl coenzyme-A carboxylase; WAT, weeks after treatment. INTRODUCTION Johnsongrass ... ...
... words: ACCase inhibitors, herbicide resistance. Abbreviations: ACCase, acetyl coenzyme-A carboxylase; AOPP, aryloxyphenoxypropionate; CHD, cyclohexanedione. Introduction The ... ...
Phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Azotobacter vinelandii, like the corresponding enzyme from other organisms, is activated by acetyl coenzyme A and inhibited by l-aspartate. Both modifiers affect primarily the affinity of the enzyme for phosphoenolpyruvate. This is the first enzyme with a strictly anaplerotic (intermediate-replacing) ...
PubMed Central
Submerged cells of the basidiomycete Nidula niveo-tomentosa, a microbial producer of 4-(4-hydroxyphenyl)-butan-2-one, were supplemented with (13)C-labeled L-phenylalanines and with [1-(13)C]glucose. Labeled transformation products were detected by a novel method of analyzing stable isotope-labeled metabolites, gas chromatography (GC) coupled to an atomic emission detector, and by GC-mass ...
PubMed
Submerged cells of the basidiomycete Nidula niveo-tomentosa, a microbial producer of 4-(4-hydroxyphenyl)-butan-2-one, were supplemented with 13C-labeled l-phenylalanines and with [1-13C]glucose. Labeled transformation products were detected by a novel method of analyzing stable isotope-labeled metabolites, gas chromatography (GC) coupled to an atomic ...
One consequence of the dramatic rise of antibiotic-resistant pathogenic bacteria is the need for new targets for antibiotics. Because membrane lipid biogenesis is essential for bacterial growth, enzymes of the fatty acid biosynthetic pathway offer attractive possibilities for the development of new antibiotics. Acetyl-coenzyme A carboxylase (ACC) catalyzes ...
In polyacrylamide gel electrophoresis in .the presence of sodium dodecyl sulfate , the molecular ..... Polyacrylamide gel electrophoresis of aerobic ACS. ...
NASA Website
Biotin has a profound effect on the metabolism of rhizobia. It is reported here that the activities of the biotin-dependent enzymes acetyl-coenzyme A carboxylase (ACC; EC 6.4.1.2) and propionyl-coenzyme A carboxylase (PCC; EC 6.4.1.3) are present in all species of the five genera comprising the Rhizobiaceae which were examined. ...
Two trials were undertaken with European seabass (Dicentrarchus labrax) to estimate the protein requirements for maintenance and growth as well as the effect of dietary protein level on the activity of hepatic acetyl coenzyme-A carboxylase (ACoAC). Six diets were formulated to contain graded levels of protein (from 5 to 55% crude ...
The biotin carboxylase (AccC) is part of the multi-component bacterial acetyl coenzyme-A carboxylase (ACCase) and is essential for pathogen survival. We describe herein the affinity optimization of an initial hit to give 2-(2-chlorobenzylamino)-1-(cyclohexylmethyl)-1H-benzo[d]imidazole-5-carboxamide (1), which was ...
Energy Citations Database
Bacterial acetyl-coenzyme A carboxylase (ACCase) is a multicomponent system composed of AccA, AccD, AccC, and AccB (also known as BCCP), which is required for fatty acid biosynthesis. It is essential for cell growth and has been chemically validated as a target for antimicrobial drug discovery. To identify ACCase inhibitors, a simple and robust assay that ...
Identification of genetic targets able to bring about changes to the metabolite profiles of microorganisms continues to be a challenging task. We have independently developed a cipher of evolutionary design (CiED) to identify genetic perturbations, such as gene deletions and other network modifications, that result in optimal phenotypes for the production of end products, such as recombinant ...
A mitochondrial model of gluconeogenesis and the tricarboxylic acid cycle, where pyruvate is metabolized via pyruvate carboxylase and pyruvate dehydrogenase, and pyruvate kinase is examined. The effect of the rate of tricarboxylic acid flux and the rates of the three reactions of pyruvate metabolism on the labeling patterns from (/sup 14/C)pyruvate and (/sup 14/C)acetate are ...
Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. These enzymes include ATP-citrate lyase (ACL), acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), and glycerol-3-phosphate acyltransferase (G3PA). ...
Dideoxykanamycin B was inactivated by an enzyme isolated from P. aeruginosa cape 15 in the presence of ATP, magnesium acetate and coenzyme A. This inactivation did not take place without either ATP or magnesium ion but the activity was restored by the add...
National Technical Information Service (NTIS)
Pyruvate carboxylase (PYC), a biotin-dependent enzyme which catalyzes the conversion of pyruvate to oxaloacetate, was hypothesized to play an important anaplerotic role in the growth of Rhizobium etli during serial subcultivation in minimal media containing succinate (S. Encarnaci�n, M. Dunn, K. Willms, and J. Mora, J. Bacteriol. 177:3058-3066, 1995). R. etli and R. tropici ...
... words: Resistance test, aryloxyphenoxypropanoate, phenylurea. Abbreviations: ACCase, acetyl coenzyme A carboxylase (EC 6.4.1.2); APP, ... al. 1993; Saari et al. 1994) or acetyl coenzyme A carboxylase (ACC...
Sodium dodecyl sulfate-denatured biotinyl proteins will bind (/sup 14/C)methyl avidin which remains bound through polyacrylamide gel electrophoresis. The method has been used to demonstrate the presence of two high molecular weight subunit forms of acetyl CoA carboxylase in rat liver cytoplasm, both of which are precipitated by antibody to purifed rat ...
A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives thereof which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides.
A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives are disclosed which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides. 5 figs.
DOEpatents
... differed slightly in the specific activity of acetyl�coenzyme A carboxylase (ACCase) (EC 6.4.1.2) ... compounds are potent inhibitors of the enzyme acetyl�coenzyme A (CoA) carboxylase (ACCase) (Gronwald 1...
