Purified T. reesei RUT C-30 acetyl esterase catalyzes acetyl transfer to a variety of carbohydrates in water in the presence of vinyl acetate as the acetyl group donor. The degree of conversion and the number of formed acetates depended on the acceptor used. With some acceptors, such as ...

... alpha(1 to 3)-D-galactosidase, alpha(1 to 3)-N-acetyl-D-galactosaminidase and alpha(1 to 2)L-fucosidase, on the blood group substances and ...

The stereoselectivity of glycosylation reactions is affected by many factors. Synthesis of 1,2-cis glycosidic linkages (such as ? linkages in glucose and galactose like monosaccharides) is challenging due to lack of control of the stereoselectivity. Our systematic study of GalN(3) donors with different combination of protecting groups indicated that ...

The pyruvate dehydrogenase component (E1) of the pyruvate dehydrogenase complex catalyzes the decomposition of 3-fluoropyruvate to CO/sub 2/, fluoride anion, and acetate. Acetylthiamin pyrophosphate (acetyl-TPP) is an intermediate in this reaction. Incubation of the pyruvate dehydrogenase complex with 3-fluoro(1,2-/sup 14/)pyruvate, TPP, coenzyme A (CoASH), and either NADH or ...

... Abstract : The presence of acetyl groups in vitellenin was confirmed by hydrazinolysis according to the DNP method of Phillips. ...

Efficient sialylations using N-glycolylneuraminic acid (Neu5Gc) phosphite donors having an acetyl or benzyl group on the glycolyl moiety are described in the synthesis of Neu5Gc-containing glycans. Both phosphite donors 1 and 2 were readily coupled with primary and secondary acceptor alcohols in propionitrile at ...

... Bovine Donor Cells for Nuclear Transfer: Levels of Histone Acetylation1B. P. Enright, B. S. Jeong, X. Yang, ... potential of cloned embryos. Because acetylation of the histones on nuclear chromatin is an ...

The distinct biochemical function of endoplasmic reticulum (ER) protein Calreticulin (CR) catalyzing the transfer of acyl group from acyloxycoumarin to a receptor protein was termed calreticulin transacylase (CRTAase). The present study, unlike the previous reports of others utilizing CR-deficient cells alone, dealt with the recombinant CR domains of Heamonchus contortus ...

Carbon monoxide dehydrogenase/acetyl-CoA synthase catalyzes acetyl-CoA synthesis from CO, CoA, and a methylated corrinoid iron-sulfur protein, which acts as a methyl donor. This reaction is the last step in the Wood-Ljungdahl pathway of anaerobic carbon fixation. The binding sequence for the three substrates has been debated for over a ...

Carbon monoxide dehydrogenase/acetyl-CoA synthase catalyzes acetyl-CoA synthesis from CO, CoA, and a methylated corrinoid iron-sulfur protein, which acts as a methyl donor. This reaction is the last step in the Wood-Ljungdahl pathway of anaerobic carbon fixation. The binding sequence for the three ...

... fertilization, but they also had higher levels of histone acetylation. Although 5-aza-dC at 0.04 μM or higher reduced DNA methylation and histone acetylation levels to those of in vitro-fertilized ... ...

SummaryThe pyruvate dehydrogenase multienzyme assembly (PDH) generates acetyl coenzyme A and reducing equivalents from pyruvate in a multiple-step process that is a nexus of central metabolism. We report crystal structures of the Geobacillus stearothermophilus PDH E1p subunit with ligands that mimic the prereaction complex and the postdecarboxylation product. The structures ...

... Lysine acetylation or Nε-acetylation, identified initially on core histones in 1968 [6], is mediated by a group of enzymes called histone ...

Enhanced expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) and 9-O-acetylated disialoganglioside (9-OAcGD3) was observed on lymphoblasts of childhood acute lymphoblastic leukemia (ALL). Sialate-O-acetyltransferase (SOAT) and sialate-O-acetylesterase (SIAE) are the two main enzymes responsible for the quantity of the ...

A novel 1,2-cis stereoselective synthesis of protected ?-D-Gal-(1?2)-D-Glc fragments was developed. Methyl 2-O-acetyl-3-O-allyl-4,6-O-benzylidene-?-D-galactopyranosyl-(1?2)-3-O-benzoyl-4,6-O-benzylidene-?-D-glucopyranoside (13), methyl 2-O-acetyl-3-O-allyl-4,6-O-benzylidene-?-D-galactopyranosyl-(1?2)-3,4,6-tri-O-benzoyl-?-D-glucopyranoside (15), methyl ...

Summary: Major aspects of the pathway of de novo arginine biosynthesis via acetylated intermediates in microorganisms must be revised in light of recent enzymatic and genomic investigations. The enzyme N-acetylglutamate synthase (NAGS), which used to be considered responsible for the first committed step of the pathway, is present in a limited number of bacterial phyla only ...

Soret resonance, QX resonance, and QY near-infrared Fourier transform (FT) (pre)resonance Raman spectroscopies were used to determine pigment-protein interactions of specific bacteriochlorin molecules in the reaction center from Chloroflexus aurantiacus. FT Raman spectroscopy, using 1064 nm excitation, was used to selectively obtain preresonance and resonance vibrational Raman spectra of the ...

Histone acetyltransferases (HATs) catalyze the transfer of an acetyl group from an acetyl-coenzyme A donor molecule to specific lysine residues within proteins. The acetylation state of proteins, particularly histones, is known to modulate their intermolecular binding properties and control ...

Heliobacteria are a recently uncovered class of photosynthetic bacteria comprised of a novel chromophore, bacteriochlorophyll (BChl) g. Only the substitution of a vinyl; group for an acetyl group on ring I distinguishes the macrocycle of BChl g from that of the more common BChl b. The different substituents impart small differences of ...

Taxol 2'-acetate, an analog of the antitumor drug taxol, displays no significant in vitro microtubule polymerization activity, thus underscoring the importance of a free 2'-OH group to the biological activity of taxol. Previous work had suggested that the inactivity of taxol 2'-acetate is not due to steric interference by the acetyl ...

... a nitrosothiol donor S-nitroso-N- acetyl-dl-penicillamine, as described previously (Liu et al., 1996). The ... ...

A new enzyme, acetate:SH-[acyl-carrier-protein] enzyme ligase (AMP), has been purified about 30-fold from cell-free extracts of Klebsiella aerogenes. The enzyme, in the presence of acetate and ATP, catalyzes the acetylation of enzymically inactive des-acetyl citrate lyase to enzymically active citrate lyase (EC 4.1.3.6). Acetate and ATP could be replaced ...

N-Acetyl-lactosamine(beta-D-Gal p-(1-->4)-D-Glc pNAc) was synthesized regioselectively with the aid of the transglycosylation activity of beta-galactosidase isolated from Diplococcus pneumoniae using p-nitrophenyl beta-D-galactopyranoside as the donor. Also, transglycosylation of the sialyl group in an alpha-(2-->8)-linked sialic ...

1. An arylalkylamine N-acetyltransferase (NAT) of the parasitic nematode, Ascaridia galli was studied using either [14C]serotonin (5-HT) or [14C]octopamine (OA) as substrates and with acetyl-CoA as the donor of the acetate group. 2. The NAT activity towards 5-HT and OA co-eluted from a size-exclusion column and appeared to have an M(r) ...

Incubation of HbS (or HbA) with aspirin leads to incorporation of acetyl groups into the protein. Incorporation was followed by the use of aspirin labeled with ¹⁴C in the acetyl group. The acetylated hemoglobins show an increase in oxygen affinity compared to the parent ...

The enzyme catalyzes the decarboxylation of pyruvate followed by a reductive acetylation of lipoyl groups of the dihydrolipoamide . ...

The enzyme catalyzes the decarboxylation of pyruvate followed by a reductive acetylation of lipoyl groups of the dihydrolipoamide . ...

The enzyme catalyzes the decarboxylation of pyruvate followed by a reductive acetylation of lipoyl groups of the dihydrolipoamide . ...

The final steps in the synthesis of acetyl-CoA by CO dehydrogenase (CODH) have been studied by following the exchange reaction between CoA and the CoA moiety of acetyl-CoA. This reaction had been studied earlier (Pezacka, E., and Wood, H. G. (1986) J. Biol. Chem. 261, 1609-1615 and Ramer, W. E., Raybuck, S. A., Orme-Johnson, W. H., and Walsh, C. T. (1989) ...

A combined docking and molecular dynamics protocol was applied to investigate quercetin binding modes within the catalytic cavity of Candida antarctica lipase B (CALB) and Pseudomonas cepacia lipase (PCL), aiming to explain the difference of specificity of these enzymes in acetylation reaction. For both lipases, docking of quercetin yielded two families of conformers with ...

A number of o-hydroxyacetylnitrobenzenes have been synthesized and their structures (X-ray and/or ab initio DFT calculations) have been determined. The intramolecular hydrogen bonds show correlations between the distances: O?O, O H, CO and O?H for both hydrogen bonds to acetyl and nitro groups. Rather short O?O distances and strong hydrogen bonds are ...

... in nature in microbes, plants, and animals, have a common enzymatic function (transfer of an acetyl group from acetyl CoA to a substrate) but differ ...

BackgroundAberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (?-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive ...

Biosynthesis of the hydroxamate-type siderophore erythrochelin requires the generation of ?-N-acetyl-?-N-hydroxy-l-ornithine (l-haOrn), which is incorporated into the tetrapeptide at positions 1 and 4. Bioinformatic analysis revealed the FAD-dependent monooxygenase EtcB and the bifunctional malonyl-CoA decarboxylase/acetyltransferase Mcd to be putatively involved in the ...

Proteins can be acetylated at the alpha-amino group of the N-terminal amino acid (methionine or the penultimate amino acid after methionine removal) or at the epsilon-amino group of internal lysines. In eukaryotes the majority of proteins are N-terminally acetylated, while this is extremely rare in bacteria. A ...

A group of 54 patients with rheumatoid arthritis (31 fast, 23 slow acetylators) treated with sulphasalazine 3 g/day were studied retrospectively. At 24 weeks no difference in the efficacy of the drug could be shown between fast and slow acetylators. In a second prospective study 40 fast acetylators were allocated ...

One of the approaches to increasing the bioavailability of resveratrol is to protect its 3-OH phenolic group. In this work, regioselective acylation of resveratrol at 3-OH was achieved by transesterification with vinyl acetate catalyzed by immobilized lipase from Alcaligenes sp. (lipase QLG). The maximum yield of 3-O-acetylresveratrol was approximately 75%, as the lipase also ...

Recent experimental data point to an asymmetric ground-state electronic distribution in the special pair (P) of purple bacterial reaction centers, which acts as the primary electron donor in photosynthesis. We have performed a density functional theory investigation on an extended model including the bacteriochlorophyll dimer and a few relevant surrounding residues to explore ...

During cold storage of donor hearts, reactive oxygen species produced by intracellular redox-active chelatable iron potentially alter myocardial function. To reduce this cold-induced injury we investigated the efficacy of two new modifications of the well established histidine-tryptophan-ketogluterate (HTK) solution (Custodiol) with the addition of ...

The effects of multiple changes in hydrogen bond interactions between the electron donor, a bacteriochlorophyll dimer, and histidine residues in the reaction center from Rhodobacter sphaeroides have been investigated. Site-directed mutations were designed to add or remove hydrogen bonds between the 2-acetyl groups of the dimer and ...

Polystyrene divinylbenzene was modified by acetyl, sulfonic acid, and quaternary ammonium groups. A resin functionalized with an acetyl group was impregnated in a PTFE membrane and used to extract and concentrate phenolic compounds from aqueous samples. T...

The objective was to determine whether alterations of histone acetylation status in donor cells affected inter-generic SCNT (igSCNT)-cloned embryo development. Leopard cat cells were treated with trichostatin A (TSA; a histone deacetylase inhibitor) for 48 h, and then donor cells were transferred into enucleated oocytes from domestic ...

Methyl l-glycero-?-d-manno-heptopyranoside was synthesized in good yield by a Fischer-type glycosylation of the heptopyranose with methanol in the presence of cation-exchange resin under reflux and microwave conditions, respectively. The compound crystallized from 2-propanol in an orthorhombic lattice of space group P2(1)2(1)2 showing a comparatively porous structure with a ...

Donor conception research supports open-identity donor programmes and disclosure to donor-conceived offspring. This study examines Australian donors', recipients' and donor-conceived offspring's views on the importance of different types of biographical information about the ...

Protein acetylation is a common modification that plays a central role in several cellular processes. The most widely used methods to study these modifications are either based on the detection of radioactively acetylated oligopetide products or an enzyme-coupled reaction measuring conversion of the acetyl donor ...

Acetylation was used as a means of measuring quantitatively the fraction of acidic protons in coal derived products. The similarity of the fractions of acidic protons measured by acetylation techniques with those of acid-base titration data suggested that no alcoholic groups were present in the SRL and SRC samples. The ...

The presence of acetyl groups in vitellenin was confirmed by hydrazinolysis according to the DNP method of Phillips. After hydrazinolysis of 10-30 mg of vitellenin at 100C for 17 hours, acetic acid DNP-hydrazide was identified by chromatography. Since no ...

... 3-D-Galactose unit, the 6-CH3 groups of the two fucose units, and the N-acetyl group of the subterminal P3-DN-acetyl-glucosamine (PDGIcNAc) (6 ...

BackgroundAlthough donor dopamine treatment reduces the requirement for post transplantation dialysis in renal transplant recipients, implementation of dopamine in donor management is hampered by its hemodynamic side-effects. Therefore novel dopamine derivatives lacking any hemodynamic actions and yet are more efficacious in protecting tissue from cold ...

An improved method is presented for producing normorphine from morphine. Morphine as the starting material is acetylated by treatment with acetylating agents to produce di-acetyl morphine (heroin). The acetylated compound is reacted with cyanating agents to produce ...

Structural and biochemical analyses reveal how ornithine acetyl-transferases catalyse the reversible transfer of an acetyl-group from a basic (ornithine) to an acidic (glutamate) amino acid by employing a common mechanism involving an acetyl-enzyme intermediate but using different side chain binding modes. PMID:21796301

enzyme catalyzes the decarboxylation of pyruvate followed by a reductive acetylation of lipoyl groups of the dihydrolipoamide acetyltransferase component of ...

Acetylation of trypsin in dimethyl sulfoxide solutions is a reaction highly specific for the free amino groups of the enzyme. The progressing acetylation causes a gradual reduction of the activity characterized by a complete inactivation upon the introduction of abcut 15 acetyl groups ...

... Specifically, experiments were proposed to assess the role of histone acetylation and high mobility group (HMG) chromatin proteins in estrogen ...

... Abstract : The polysaccharide of Bacillus anthracis is shown to be a branched chain of molecules of N-acetyl-D-glucosamine, to which are joined ...

In the title compound, C(10)H(11)NO(2)S, the acetyl-acetone group crystallizes in the keto form with all the non-hydrogen atoms in the acetyl-acetone group approximately co-planar with a maximum atomic deviation 0.055?(2)?�; the dihedral angle between the acetyl-acetone ...

Previous structure-activity relationship studies of adenophostin A, a potent IP(3) receptor agonist, led us to design the novel adenophostin A analogues 5a-c, conjugating an aromatic group at the 5'-position to develop useful IP(3) receptor ligands. The common key intermediate, a D-ribosyl alpha-D-glucoside 10alpha, was stereoselectively synthesized by a glycosidation with the ...

An accepted definition of donor exclusion criteria has not been established for living donor liver transplantation (LDLT). The use of elderly donors to expand the living donor pool raises ethical concerns about donor safety. The aims of this study were (1) the comparison of the postoperative ...

We report NMR studies of transient hydrogen bonding in a polysaccharide dissolved in water without co-solvent at ambient temperature. The polysaccharide portion of the Escherichia coli O142 lipopolysaccharide is comprised of repeating pentasaccharide units of GalNAc (N-acetyl galactosamine), GlcNAc (N-acetyl glucosamine) and rhamnose in a 3:1:1 ratio, ...

We had previously reported on the purification of the alpha(1 to 3)-N-acetyl-galactosaminidase from Cl. perfringens (8000 fold) and of the alpha(1 to 3)-galactosidases, B-zyme, from Cl. sporogenes (2500 fold). Both enzymes were, nonetheless demonstrated t...

... organization. Trichostatin A (TSA; 0�5 ?M), a histone deacetylase inhibitor, was used to increase histone acetylation and 5-aza-2?-deoxycytidine (5-aza- ... ...

The biochemical basis underlying the genetic polymorphism of drug N-acetylation was investigated using a combination of in vivo and in vitro assays for arylamine N-acetyltransferase (NAT) activity and content in human liver. The acetylator phenotype of 26 surgical patients was determined using caffeine as an innocuous probe drug by measurement of the ...

The major pathway for INH metabolism is acetylation of acetyloisoniazid by a noninducible hepatic enzyme N-acetyltransferase. Examples of drugs acetylated by this enzymatic system are: isoniazid, sulphadimidine, hydralazine, dapson and sulphapiridine. The rate of acetylation is constant in any individual but varies in different ...

Background:Few studies have been done on the relation between acetylator status and allergic diseases.Aim:To determine any possible association between acetylating phenotype in pediatric patients with atopic dermatitis (AD) and the disease prognosis.Patients and Methods:Thirty-six pediatric patients and forty two healthy children as a control ...

... Accession Number : AD0715696. Title : Tissue Transplantation: The Universal Donor and Blood Group Antibodies. Descriptive Note : Progress rept., ...

The chemical structure of the surface polysaccharide from Staphylococcus aureus M was investigated by a combination of methanolytic, hydrolytic, and chromatographic techniques. The repeating unit that was most consistent with the data was a hexasaccharide composed of N-acetyl-D-aminogalacturonic acid, N-acetyl-D-fucosamine, and taurine in molar ratios of ...

Rats were exposed to whole-body x radiation and killed at intervals of 2, 12, and 24 h later. Histones were separated from purified nuclei of liver cells. The intensity of histone acetylation was assessed according to incorporation of acetyl groups from /sup 14/CH/sub 3/COOH injected intraperitoneally. A graph is presented to show ...

Molecular dynamics simulations have been carried out to characterize the structure and solubility of chitosan nanoparticle-like structures as a function of the deacetylation level (0, 40, 60, and 100%) and the spatial distribution of the N-acetyl groups in the particles. The polysaccharide chains of highly N-deacetylated particles where the ...

To expand the donor liver pool, ways are sought to better define the limits of marginally transplantable organs. The Donor Risk Index (DRI) lists 7 donor characteristics, together with cold ischemia time and location of the donor, as risk factors for graft failure. We hypothesized that donor ...