... Acetyl-coa Carboxylase (ACCase) Inhibitor�Resistant Wild Oat (Avena fatua) Biotypes in the Pacific NorthwestAhmet Uludag, Kee Woong ... diclofop, fenoxaprop, pinoxaden, quizalofop, sethoxydim, tralkoxydim...
NBII National Biological Information Infrastructure
A novel mutant of Arabidopsis thaliana having highly glossy inflorescence stems, post-genital fusion in floral organs, and reduced fertility, was isolated from an EMS-mutagenized population and designated glossyhead1 (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gene identified as a new allele of Acetyl-CoA Carboxylase1 (ACC1), a ...
PubMed
... related to pyruvate carboxylase deficiency? Mutations in the PC gene cause pyruvate carboxylase deficiency. The PC gene provides instructions for making an enzyme called ...
MedlinePLUS
A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives thereof which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides.
Energy Citations Database
A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives are disclosed which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides. 5 figs.
DOEpatents
... of the large subunit of ribulose-1, 5-biphosphate carboxylase/oxygenase gene (rbcL), Gavio et al. (2005, ... ...
Comparative genomics is a useful tool to investigate gene and genome evolution. Biotin carboxylase (BC), an important subunit of heteromeric ACCase that is a rate-limiting enzyme in fatty acid biosynthesis in dicots, catalyzes ATP, biotin-carboxyl-carrier protein and CO2 to form carboxybiotin-carbo...
Technology Transfer Automated Retrieval System (TEKTRAN)
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) [sup [epsilon
... variation in a rare, endemic plant, D. newtonianum. Phosphoenolpyruvate carboxylase, or PepC, is an enzyme coded for ... in the 4th Intron of the nuclear gene Phosphoenolpyruvate carboxylase. (*) BUF �=� ...
Carbon dioxide limitation of Sulfolobus metallicus resulted in increased cellular concentrations of polypeptides that were predicted to be biotin carboxylase and biotin carboxyl-carrier-protein components of a protein complex. These polypeptides were coeluted from a native polyacrylamide gel and were estimated at 19 and 59 kDa after separation by denaturing gel ...
The first prokaryotic urea carboxylase has previously been purified and characterized from Oleomonas sagaranensis. As the results indicated the presence of an ATP-dependent urea degradation pathway in Bacteria, the characterization of the second component of this pathway, allophanate hydrolase, was carried out. The gene encoding allophanate hydrolase was ...
The ribulose bisphosphate carboxylase (RuBPC) small subunit gene family in soybean is being examined as a source of strong transcriptional control signals in plants. Six new RuBPC small subunit recombinants have been isolated from two new soybean DNA libr...
National Technical Information Service (NTIS)
... were observed between transgenic maize hybrids with the phosphoenolpyruvate carboxylase promoter expressing the same cry1Ab genes or ... ...
The expression of two closely related soybean ribulose bisphosphate carboxylase small subunit (Rubisco ss) genes, SRS1 and SRS4, has been compared. These genes account for approximately 2-4% of the total transcription in light grown leaves, SRS4 being twi...
BackgroundUrea amidolyase breaks down urea into ammonia and carbon dioxide in a two-step process, while another enzyme, urease, does this in a one step-process. Urea amidolyase has been found only in some fungal species among eukaryotes. It contains two major domains: the amidase and urea carboxylase domains. A shorter form of urea amidolyase is known as urea ...
PubMed Central
SRS1 and SRS4 are two closely related soybean ribulose -1,5-bisphosphate carboxylase small subunit (SSU) genes. The promoters from both SRS1 and SRS4 can be fused to a neomycin phosphotransferase gene and these fusions produce similar levels of kanamycin ...
The gene coding for ribulose-1,5-bisphosphate carboxylase (RuBisCO; 3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39) was cloned from a sulfur-oxidizing chemoautotrophic bacterium that resides as an endosymbiont within the gill tissues of Alv...
The gene coding for ribulose-1,5-bisphosphate carboxylase was from a sulfur-oxidizing chemoautotrophic bacterium that resides as an endosymbiont within the gill tissues of Alvinoconcha hessleri, a gastropod inhabiting deep-sea hydrothermal vents. Nucleoti...
Two pea nuclear genes encoding ribulose-1,5-bisphosphate carboxylase (rbcS) were isolated and completely sequenced. These sequence studies include approximately 1 kb of 5' noncoding region and several hundred nucleotides of 3' noncoding sequences. The two...
The gene for ribulose-bisphosphate carboxylase was characterized for several chemoautotropic bacterial symbionts. The gene of the symbiont of the deep-sea snail Alvinochonca hessleri has been largely sequenced and compared to the sequences of other photo-...
An international workshop on the properties of the ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and its involvement in photosynthesis was held in Tucson, AZ on April 20-25, 1987. Attending were 100 participants with 42 invited speakers coming...
cDNA clones from pea encoding the small subunit of RuBP carboxylase and the chlorophyll a/b binding protein were characterized by translation of hybrid-selected mRNAs. The translation products were identified by SDS-polyacrylamide gel electrophoresis and ...
A genetic locus essential for the formate-dependent growth of Bradyrhizobium japonicum was isolated by complementation of ethyl methanesulfonate-induced mutants with a cosmid gene library of B. japonicum DNA. Three related cosmids containing 18.7 kilobase pairs of B. japonicum DNA in common were identified as being able to restore formate-dependent growth capability to mutants ...
The sulfate-reducing highly enriched culture N47 is capable to anaerobically degrade naphthalene, 2-methylnaphthalene, and 2-naphthoic acid. A proteogenomic investigation was performed to elucidate the initial activation reaction of anaerobic naphthalene degradation. This lead to the identification of an alpha-subunit of a carboxylase protein that was two-fold up-regulated in ...
The effects of white light and far-red light on transcription rates and steady state mRNA levels of two closely related soybean RuBPCss genes, SRS1 and SRS4, have revealed a system of light regulated RNA turnover. Transcription of these genes is 30 to 50 ...
The ribulose bisphosphate carboxylase (RuBPC) small subunit gene family in soybean is being examined as a source of strong transcriptional control signals in plants. Six new RuBPC small subunit recombinants have been isolated from two new soybean DNA libraries. These clones are being characterized and compared to existing clones. Portions of a second ...
We identified the first prokaryotic urea carboxylase (UCA) from a member of the alpha subclass of the class Proteobacteria, Oleomonas sagaranensis. This enzyme (O. sagaranensis Uca) was composed of 1,171 amino acids, and its N-terminal region resembled the biotin carboxylase domains of various biotin-dependent carboxylases. The ...
The goals of this project were: To developmolecular biological approaches.for use in environmental science using Ribulose 1,5-bisphosphate carboxylase (Rubisco) genes as probes for autotrophy. To understand the Mn cycle and its interrelationships with oth...
To develop molecular biological approaches for use in environmental science using Ribulose 1,5-bisphosphate carboxylase (RubisCo) genes as probes for autotrophy. To understand the Mn cycle and its interrelationships with other biological and geochemical p...
... reported for the facultatively cross-pollinating species kochia [Kochia scoparia (L.) Schrad.] (Stallings et al. 1995) and for ... Pollen-mediated gene flow of sulfonylurea-resistant kochia (Kochia scopar...
... Berliner cry1Ab gene under control of the maize phosphoenolpyruvate carboxylase (PEPC) promoter was evaluated against four lepidopterous ... a maize pollen-specific promoter and the maize phosphoenolpyruv...
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltransferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of ...
DOE Information Bridge
The genes for the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase from the cyanobacterium Anacystis nidulans were subcloned into plasmid pUC9. After induction, both genes were expressed in Escherichia coli and the subunits were assembled into an active holoenzyme. The enzyme was purified from E. coli to high ...
The attachment of biotin to apocarboxylases is catalyzed by holocarboxylase synthetase (HCS). An inherited deficiency of HCS results in the disorder 'multiple carboxylase deficiency', which is characterized by reduced activity of all biotin-dependent carboxylases. Here we show that the majority of HCS localizes to the nucleus rather than the cytoplasm ...
We have used functional complementation of Escherichia coli pur mutants to clone avian cDNA encoding 5-aminoimidazole ribonucleotide (AIR) carboxylase-5-aminoimidazole-4-N-succinocarboxamide ribonucleotide (SAICAR) synthetase, the bifunctional enzyme catalyzing steps 6 and 7 in the pathway for de novo purine nucleotide synthesis. Mutational analyses have been used to establish ...
In Mesembryanthemum crystallinum, phosphoenolpyruvate carboxylase is synthesized de novo in response to osmotic stress, as part of the switch from C(3)-photosynthesis to Crassulacean acid metabolism. To better understand the environmental signals involved in this pathway, we have investigated the effects of light on the induced expression of phosphoenolpyruvate ...
An ampicillin-resistant, RecA- strain of Escherichia coli (HB101) harboring the multicopy pBR322 plasmid containing the structural gene for ribulosebisphosphate carboxylase from Rhodospirillum rubrum was used to prepare large quantities of the carboxylase protein. This recombinant system was characterized by extreme plasmid ...
Strains of Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) were constructed such that either the gene encoding form I ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBPC-O) or the gene encoding form II RuBPC-O was inactivated. Both strains were capable of photoheterotrophic growth with malate as the electron donor, with only ...
The objective of this study was to determine the role of midK, which encodes a protein similar to pyruvate carboxylase, in mimosine degradation by Rhizobium sp. strain TAL1145. The midK gene is located downstream of midR in the cluster of genes for mimosine degradation in Rhizobium sp. strain TAL1145. The midK mutants of TAL1145 ...
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is ...
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS){sup {epsilon}}N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the {epsilon}-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. ...
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) {epsilon}N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the {epsilon}-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. ...
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .epsilon.N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. ...
Holocarboxylase synthetase (HCS) catalyzes biotin incorporation into various carboxylases that require biotin as a prosthetic group. They are acetyl-CoA carboxylase, a rate-limiting enzyme of fatty acid synthesis; pyruvate carboxylase, a key enzyme of gluconeogenesis; propionyl-CoA carboxylase and ...
There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two proteins. These results are supported by limited proteolysis ...
The gene that encodes acetyl-coenzyme A carboxylase (ACCase; EC 6.4.1.2) in the eukaryotic alga Cyclotella cryptica has been isolated and cloned, representing the first time that a full-length gene for this enzyme has been isolated from a photosynthetic organism. The gene contains a 447-base pair intron that is ...
The conversion of AIR to CAIR catalyzed by AIR carboxylase represents the only carbon-carbon bond formation step in de novo purine biosynthesis. Prokaryotic and most eukaryotic AIR carboxylases utilize two proteins, PurK and PurE to accomplish the conversion of AIR to CAIR via N5-CAIR from AIR, ATP, and bicarbonate. In vertebrates, AIR ...
NASA Astrophysics Data System (ADS)
We have isolated cDNA clones for the maize leaf enzymes phosphoenolpyruvate (P-ePrv) carboxylase [orthophosphate:oxaloacetate carboxy-lyase (phosphorylating) EC 4.1.1.31] and pyruvate,orthophosphate (Prv,Pi) dikinase (ATP:pyruvate,orthophosphate phosphotransferase, EC 2.7.9.1) by exploiting the light-inducibility and large size of the mRNAs (3.5 ...
