... such as a calcium-binding protein (CB) and an enzyme for acetylcholine, which are important markers for avian sensory development. ...
NASA Website
The Neuromuscular Disease Center at Washington University provides this comprehensive Web site for information on acetylcholine and acetylcholine receptors. Visitors will find detailed Web pages covering acetylcholine metabolism, acetylcholine receptors (including disorders, structure, venom ...
NSDL National Science Digital Library
Fluorescence based screening assays are provided that allow for the for identification of agents that selectively bind to a ligand-gated ion channel (LGIC) such as a nicotinic acetylcholine receptor (nAChR). Also provided are methods for identifying agent...
National Technical Information Service (NTIS)
The nicotinic acetylcholine receptor is a prototype ligand-gated ion channel that mediates signal transduction in the neuromuscular junctions and other cholinergic synapses. The molecular basis for the energetics of ligand binding and unbinding is critical to our understanding of the pharmacology of this class of receptors. Here we used steered molecular ...
PubMed Central
... Title : ACETYLCHOLINE RECEPTOR BINDING CHARACTERISTICS OF SNAKE AND CONE SNAIL VENOM POSTSYNAPTIC NEUROTOXINS ...
DTIC Science & Technology
Histrionicotoxin from the Colombian frog Dendrobates histrionicus and its perhydro derivative reversibly block the acetylcholine-sensitive ion conductance system in frog neuromuscular preparations. The perhydro derivative and [3H]perhydrohistrionicotoxin, like histrionicotoxin, caused a significant decrease in the peak amplitude of the end-plate current and shortened its rise ...
PubMed
... Title : Activation, Inactivation, and Desensitization of Acetylcholine Receptor Channel Complex Detected by Binding of Perhydrohistrionicotoxin. ...
... Title : Acetylcholine Receptor Binding Characteristics of Snake and Cone Snail Venom Postsynaptic Neurotoxins: Further Studies with a Non ...
Numerous high-resolution crystallographic structures of the acetylcholine binding protein (AChBP), a molluscan cholinergic protein, homologous to the extracellular domain of nicotinic acetylcholine receptors, are available. This offers opportunities to model the interaction between various ...
... Accession Number : ADA113141. Title : Structural Models of the Nicotinic Acetylcholine Receptor and Its Toxin-Binding Sites. ...
... Accession Number : ADA299841. Title : Sensitivity Increase in Nicotinic Acetylcholine Receptor Binding in Fiber Optic Wave Guide. ...
... ACETYLCHOLINE RECEP'TORVN~tNNG-CHARkACTERISTICS OF SNAKE AND CONE SNAIL VENOM POSTSYNAPTIC NEUROTOXINS ...
the Lymnaea stagnalis acetylcholine binding protein (AChBP) and the trans- membrane (TM) domain of the TorpedoAChRs during the gating process is currently unclear. Recently, the structure of nAChR from Torpedo marmorata-bound AChBP structure into the electron density of Torpedo nAChR, Unwin et al. were able to suggest how
E-print Network
... Accession Number : ADP008893. Title : Protein Phosphorylation and Inhibition of Acetylcholine Release by Botulinum Neurotoxin A. ...
Surface plasmon resonance (SPR) biosensors recently gained an important place in drug discovery. Here we present a primary and secondary SPR biosensor screening methodology. The primary screening method is based on a direct binding assay with covalent immobilized drug target proteins. For the secondary screening method, a sequential competition assay has ...
Black lipid membranes were formed with oxidized cholesterol in the presence of either the acetylcholine receptor, purified from the electric organ of the electric ray Torpedo californica or its tryptic digest. In both cases, conductance of cations increased and was dependent on the concentration of the receptor protein. Conductance of Ca/sup + +/ was ...
Energy Citations Database
The GA-binding protein (GABP) transcription factor has been shown in vitro to regulate the expression of the neuromuscular proteins utrophin, acetylcholine esterase, and acetylcholine receptor subunits ? and ? through the N-box promoter motif (5?-CCGGAA-3?), but its in vivo function remains ...
The major brain nicotinic acetylcholine receptor is assembled from two subunits termed ? 4 and n? 1. When expressed in Xenopus oocytes, these subunits reconstitute a functional acetylcholine receptor that is inhibited by progesterone levels similar to those found in serum. In this report, we show that the steroid interacts with a site located on the ...
NASA Astrophysics Data System (ADS)
?-Bungarotoxin (?-bgtx) binding proteins, including certain nicotinic acetylcholine receptors and acetylcholine-binding proteins (AChBPs), are frequently characterized with radioisotope-labeled ?-bgtx-binding assays. Such assays, however, preclude investigations of ...
In a continuing study of nicotine binding sites, the authors determined the relative amount of nicotine binding and acetylcholine binding in various brain regions of C57/BL and of DBA mice. Although midbrain showed the highest and cerebellum the lowest binding for both (/sup 3/H)nicotine and ...
The temperature dependence of agonist binding and channel gating were measured for wild-type adult neuromuscular acetylcholine receptors activated by acetylcholine, carbamylcholine, or choline. With acetylcholine, temperature changed the gating rate constants (Q(10) ? 3.2) but had almost no effect on the ...
BackgroundNicotine is a psychoactive drug presenting a diverse array of biological activities, some positive, such as enhancement of cognitive performances, others negative, such as addiction liability. Ligands that discriminate between the different isotypes of nicotinic acetylcholine receptors (nAChRs) could present improved pharmacology and toxicity profile.ResultsBased on ...
