... 1984. A com- parison of eel electroplax and snake venom acetylcholin- esterase. ... A new and rapid determination of acetylcholin- esterase activity. ...
DTIC Science & Technology
... 7 List of figures 1. The effect of photoperiod on blood acetylcholin- esterase activity in cats determined at 4-hour intervals. ...
... The effect of subacute administration of Phy and trained exercise on choline acetyltransferase (ChAT) and acetylcholin- esterase (AChE) activities ...
Modern views on the structure of cholinesterase active sites and the mechanism of their interaction with organophosphorus inhibitors are considered. The attention is focused on the mechanism of the effect of cholinergic receptor blockers, acetylcholine antagonists, on the rate of interaction of acetylcholine ...
NASA Astrophysics Data System (ADS)
... C.-Y., and Wu, M.-T. (1986) Kinetic studies and strcture activity relationships akbispyddinium oximes Srseactivator of acetylcholin-esterase inhibited ...
... (40) Ordentlich, A.; Barak, D.; Kronman, C.; Ariel, N.; Segall, Y.; Valan, B.; Shafferman, A. The architecture of human acetylcholin- esterase active ...
... protein conjugate.'" and a recombinant protein;"" "' an unconjugated 25-amino acid peptide from the active site of' acetylcholin- esterase.' and tumor ...
... OFP (5 ug) reduced acetylcholin- esterase (ACHE) activity to 36, at min and to 8% after S min and remained decreased for up to 6 hrs. ...
E3 esterase (sensitive) E3 esterase (resistant) Diazinon Acetylcholine G A A M M T T I I F F H H L L V V D D Esterase activity Diazinon hydrolysis E3 sensitive E3 resistant Chimaera 1 Chimaera 2 Glu327 the evolution of resistance to diazinon in the sheep blowfly Lucilia cuprina57 . Diazinon is ...
E-print Network
... allosteric in nature (5,15). It is known that acetylcholin- esterase ... spontaneous reactivation of N,N-dimethylcarbamoyl acetylcholin- ...
The question of which cells (distant or local) contain acetylcholine esterase (AChE) activity in the hippocampal cortex, and which interneuronal contacts function with the participation of AChE is explored. Results of histochemical identification of AChE ...
National Technical Information Service (NTIS)
... UX. A HIGHLY SPECIFIC ACETYLCHOLIN- ESTERASE INHIBITOR, WAS REQUIRED AT MUCH HIGHER CONCENTRATIONS ...
... ACETYLCHOLINE, ACETYLCHOLINESTERASE, ANTIBODIES, CLONES, ESTERASES, GENETIC ENGINEERING, HUMANS, PRODUCTION ...
... and the pharmacodynamic relationship between plasma pyridostigmine concentrations and erythrocyte acetylcholin- esterase inhibition. ...
In the brain of the Caecilian species Chthonerpeton indistinctum the following enzymes have been demonstrated by means of histochemical techniques: acid phosphatase, alpha-naphthylacetate esterase, acetylcholin esterase. Acid phosphatase occurs in the cytoplasm of the neurons in 4 different types of localization. Its ...
PubMed
A novel lab-on-a-chip nanotree enzyme reactor is demonstrated for the detection of acetylcholine. The reactors are intended for use in the RISFET (regional ion sensitive field effect transistor) nanosensor, and are constructed from gold-tipped branched nanorod structures grown on SiNx-covered wafers. Two different reactors are shown: one with simple, one-dimensional nanorods ...
Organophosphate acetylcholine esterase inhibitor poisoning is a major health problem in children. We report an unusual cause of organophosphate acetylcholine esterase inhibitor poisoning. Two children were admitted to the pediatric intensive care unit due to organophosphate acetylcholine ...
PubMed Central
... Abbreviations: Ach, acetylcholine; AChE, acetylcholinesterase; AChEI, acetylcholin- esterase inhibitor; ATR, atropine sulfate; AUC, area under curve ...
... Table 1. Untreated rats, esterase aotivity Substrate Serum Brain Liver , Acetylcholin ++ ++ ... Acetylcholin + + 2+ 2+ 2+ - + + + 2+ 2+ 2+ 2+ 3+ 4+ ...
... acetyicholinesterase 24 3. Circular dichroism of detergent-soluble acetylcholin- esterase in lipid vesicle and detergent solutions at 40 C 25 ...
... The function of this isozyme is not known, whether it is the critically functional form of acetylcholin- esterase or whether it is one of the other ones we ...
... et a!. and Michel et al ' respectively. In agreement with the low acetylcholin- esterase. acvtv of homogenates of rat live; anti ...
... These agents are believed to produce their major effect by inhibiting (ie, blocking the action of) acetylcholin- esterase (ACHE), an enzyme needed ...
... A kinetic Model for Acetylcholin- on partition coefficients and metabolic constants, Mr. esterase Inhibition by Diisopropylfluorophosphate in Page 19. ...
... It is well known that DFP inhibits both serum cholinesteraae and acetylcholin - esterase, Chronic treatment with DFP requires multiple injections of ...
... Genomic cloning and RNAase digestion experiments reveal a point of divergence in the acetylcholin- esterase messages and have permitted the ...
... acetylcholin- esterase, 5-hydroxytryptophan decarboxylase, and monoamine oxidase in four species, J Neurochem 12,927-935. ...
The systematic examination of factors influencing the reactivation of phosphorylated acetylcholin-esterase has shown that only oximes with K sub a values between 7.6 and 7.9 are effective (optimum at pK sub a approximately 7.8). Oximes with a pK sub a val...
... Esterase activity is terminated by adding chloroform and releasing paranitrophenol converted to the 400 nanometers absorbing ...
1. The novel choline analogs selenonium choline (SeCh) and acetylselenonium choline (ASeCh) have been examined for selected biological activities. 2. ASeCh was found to be an alternative substrate for acetylcholine esterase with Km and Vmax values similar to acetylcholine. 3. ASeCh and SeCh inhibited ...
. The key finding of biological importance is that the rate accelerations of the monomeric and tetrameric mAChE for studies of several conformations of tetrameric mouse acetyl- cholinesterase (mAChE)(20). However, the SSSE and experimental results. AChE is a serine esterase that terminates the activity of acetylcholine (ACh) within
The US EPAs N-Methyl Carbamate Cumulative Risk Assessment (NMCRA) assesses the effect on acetylcholine esterase (AChE) activity of exposure to 10 N-methyl carbamate (NMC) pesticides through dietary, drinking water, and residential exposures....
EPA Science Inventory
The US EPA�s N-Methyl Carbamate (NMC) Cumulative Risk assessment was based on the effect on acetylcholine esterase (AChE) activity of exposure to 10 NMC pesticides through dietary, drinking water, and residential exposures, assuming the effects of joint exposure to NMCs is dose-...
THE US EPA'S N-METHYL CARBAMATE CUMULATIVE RISK ASSESSMENT (NMCRA) ASSESSES THE EFFECT ON ACETYLCHOLINE ESTERASE (AChE) ACTIVITY OF EXPOSURE TO 10 N-METHLY CARBAMATE (NMC)PESTICIDES THROUGH DIETARY, DRINKING WATER, AND RESIDENTIAL EXPOSURES. THESE DATA THUS INFORM, BUT DO NOT COM...
Experiments at elevated temperatures permit the determination of rate constant and thermodynamic activation parameters for the neutral hydrolysis of the neurotransmitter acetylcholine in water. At 25 �C, the extrapolated rate constant for the uncatalyzed (or neutral) hydrolysis of acetylcholine is 3.9 � 10(-7) s(-1) at 25 �C ...
... Title : Activation, Inactivation, and Desensitization of Acetylcholine Receptor Channel Complex Detected by Binding of Perhydrohistrionicotoxin. ...
... Title : Acetylcholine Concentration and Cholinesterase Activity in the Central Nervous System and Blood in Anaphylactic Shock,. ...
Effects of n-octane and n-nonane (1 ml per kg body weight daily) on hepatic and serum enzymes, which reflect abnormal liver function and concentration of lipid and nucleic acid (serum and liver), were studied after 2 and 7 days intraperitoneal administration to albino rats. Decreased activities of serum acetylcholine esterase and ...
... can act directly on nicotinic receptors, and others also act indirectly, like chemical warfare agents, through inhibition of acetylcholin- esterase. ...
... It has been arguedI that failure to regenerate native acetylcholin- esterase from that which has been poisoned with derivatives of methyl ...
... given in a dose that is adequate to inhibit red blood cell acetylcholin- esterase by 20-40%. Previous studies conducted at our institution determined ...
... 10:20 Coffee 10:50 BP DOCTOR (WVashington, DC) Structural and immunochevuical properties of fetal bovine serum acetylcholin esterase ...
... Nyquist-Battie, CN, Hagler, K. and Millington, WR Glycyl-L- glutamine regulates the expression of asymmetric acetylcholin- esterase molecular ...
... Page 36. Nyquist-Battie, C. Changes in the expression of acetylcholin- esterase molecular forms during rat heart development. (In Press). ...
... N,N'-Oxydimethylenebis (Pyridinium-4-Aldoxime) (Toxogonin) with Particular Reference to Their Effect on Phosphorylated Acetylcholin- esterase in ...
... 3. Roufogalis, B., and Wickson, VM, "Acetylcholin- esterase: Specificity of the Peripheral Anionic Site for Cholin- ergic Ligands," Mol. Pharm. Vol. ...
... 1, 1968, 56-59 TOPIC TAGS: toxicity, sevix, iveecticide intoxication, acetylcholin- esterase, serotonin, cholinesterase inhibitor ...
Sensitization of the organism is accompanied by a tendency toward the accumulation of acetylcholine and a reduction in the activity of cholinesterase in the brain as well as an accumulation of acetylcholine and a slight increase in cholinesterase activity...
The presence of acetylcholine in the culture medium was found to result in egg retention in the bdelloid rotifer Philodina acuticornis in a dose-dependent fashion, with no other discernible physiological effect. When six to eight eggs accumulate, the animals distend, burst and die. No other cholinergic agonist has been shown to produce a similar effect. The antagonistic effect ...
High-fluoride (100 and 200�ppm) water was administered to rats orally to study the fluoride-induced changes on the thyroid hormone status, the histopathology of discrete brain regions, the acetylcholine esterase activity, and the learning and memory abilities in multigeneration rats. Significant decrease in the serum-free thyroxine ...
Crude synaptosomal preparations of rat brain were separated by concomitant flotation and sedimentation in a sucrose gradient into fractions containing myelin, nerve endings, and mitochondria, respectively. Two myelin-free nerve ending fractions were obtained which differ in the serotonin content and the activities of the enzymes 5-hydroxytryptophan decarboxylase, monoamine ...
We investigated acetylcholine esterase (AChE) activity, acetylcholine and muscarinic M1, M3 receptors kinetics in the cerebral cortex of young and old streptozotocin induced and insulin treated diabetic rats. The role of muscarinic receptors in intracellular calcium release from pancreatic islets was studied in ...
Fresh specimens of human and rat neocortex were used to determine direct and indirect inhibitory potencies of choline esterase inhibitors (ChEIs) on ChE and the release of acetylcholine (ACh), respectively. Km values of ChE in homogenates of rat and human neocortex did not differ significantly, whereas the specific activity of ChE was ...
A bioelectronic hybrid system for the detection of acetylcholine esterase (AChE) catalytic activity was assembled by way of immobilizing the enzyme to the gate surface of an ion-sensitive field-effect transistor (ISFET). Photometric methods used to characterize bonded enzyme and linker layers on silicon substrates confirm the existence ...
Two kinetic models are introduced which predict amplitudes and time-courses of endplate currents and miniature endplate currents at neuromuscular junctions, at both normal and acetylcholinesterase-inhibited endplates. Appropriate differential rate equations reflecting interactions of acetylcholine with acetylcholine receptor and with ...
Hen brain microsomal preparation has phenyl valeratehydrolyzing activity associated with neurotoxic esterase activity. Part of that activity is due to paraoxon-insensitive esterases and a sub-part of this is sensitive to neurotoxic organophosphates, i.e.,...
We report a method for fabrication of free-standing porous gold material with high surface area, and well-defined, tunable pore morphology. Porous gold is formed via a simple procedure which involves an acidic treatment of a commercially available complex white-gold alloy. We used SEM and AFM techniques to characterize the surface morphology, size and shape of the meso-pores as well as the surface ...
Esterase activities were generated from partially denatured bovine pancreatic ribonuclease by means of conformational modification induced by known esterase-inhibitors, and by crosslinking the modified protein. A spectrophotometric method was developed to assay the emisynthetic esterases generated by the above ...
Energy Citations Database
Five mutants of Bacillus subtilis 168 defective in an intracellular esterase activity were identified. By polyacrylamide gel electrophoresis, four of the mutants were shown to lack esterase B activity, and the fifth lacked esterase A activity. All of the back-crossed ...
... Title : Changes in Extracellular Striatal Acetylcholine and Brain Seizure Activity Following Acute Exposure to Nerve Against in Freely Moving ...
Four esterase genes and general esterase activity were investigated in the gut of the termite Reticulitermes flavipes. Two genes (RfEst1 and RfEst2) share significant translated identity with a number of insect JH esterases. The two remaining genes (RfEst3 and RfEst4) apparently code for much shorte...
Technology Transfer Automated Retrieval System (TEKTRAN)
AIMS--To examine the possibility that monocyte esterase deficiency (MED) could be caused by exposure to organophosphates. METHODS--Pseudocholinesterase, paraoxonase and arylesterase activities were measured in the serum and acetylcholinesterase activity was measured in the red cells of a group of monocyte esterase ...
Thirty-five male painters in the age group of 20�50 years occupationally engaged in domestic and commercial painting for 5�12 years having blood lead levels (BLL) ?40 ?g/dl were subjected to the determination of acetyl choline esterase (AChE) levels both in plasma and red blood cell (RBC) lysate. BLL were determined using a graphite furnace atomic absorption spectrometer. ...
Brain damage due to an episode of hypoxia remains a major problem in infants causing deficit in motor and sensory function. Hypoxia leads to neuronal functional failure, cerebral palsy and neuro-developmental delay with characteristic biochemical and molecular alterations resulting in permanent or transitory neurological sequelae or even death. During neonatal hypoxia, traditional resuscitation ...
ERIC Educational Resources Information Center
Phenylmethylsulfonyl fluoride (PMSF) is known as phospholipase C inhibitor and also as acetylcholine esterase inhibitor. The purpose of this study is to examine the effect of PMSF on brain tissue arachidonic acid concentrations and extracellular glutamate levels in complete ischemia in rats. Complete cerebral ischemia was induced in rats by decapitation. ...
Neuropathy target esterase (NTE) is a membrane-associated protein with serine esterase activity. A class of organophosphate (OP) compounds, used in insecticides and as chemical weapons, are capable of inhibiting NTE and lead to progressive neuropathies. W...
Esterase activities were generated from partially denatured bovine pancreatic ribonuclease by means of conformational modification induced by known esterase-inhibitors, and by crosslinking the modified protein. A spectrophotometric method was developed to...
It is apparent that the PMN contains two esterases. One, with the characteristics similar to an acetyl esterase, exists in or on the cell in an active form. The other esterase, with some features suggestive of a chymotrypsin-like enzyme, exists in or on t...
The nerve gas sarin is a potent irreversible inhibitor of acetylcholine esterase that in high doses causes neurotoxicity seizures lung inflammation and death primarily from respiratory failure. Even in subclinical doses sarin suppresses the immune system ...
The goal of this work was to clone by DNA recombinant technology the gene for acetylcholine esterase from a human source. In order to do this we prepared and purified monoclonal antibody from large amounts of medium in which HG-72 hybridoma (ATCC) has bee...
