... Title : Activation, Inactivation, and Desensitization of Acetylcholine Receptor Channel Complex Detected by Binding of Perhydrohistrionicotoxin. ...
DTIC Science & Technology
Nicotinic acetylcholine receptors mediate fast synaptic transmission by fluxing ions across the membrane in response to neurotransmitter binding. We show here that during affinity purification of the nicotinic acetylcholine receptor from Torpedo, phosphatidic acid, but not other anionic or zwitterionic ...
PubMed
PubMed Central
... Title : Turnover of Acetylcholine Receptors: Mechanisms of Regulation. ... Acetylcholine receptors, RA5 Neuromuscular junctions, Motor nerves. ...
Modern views on the structure of cholinesterase active sites and the mechanism of their interaction with organophosphorus inhibitors are considered. The attention is focused on the mechanism of the effect of cholinergic receptor blockers, acetylcholine antagonists, on the rate of interaction of acetylcholine ...
NASA Astrophysics Data System (ADS)
... Density, Morphological Remodeling, and Butyrylcholinesterase Activity Can Sustain Muscle Function in Acetylcholinesterase Knockout Mice. ...
... may lead to useful water and oil ... of the active site with acetate substrates containing ... Sodium dodecyl sulfate (SDS) polyacrylamide gel equipment ...
A family of genes coding for proteins homologous to the ? subunit of the muscle nicotinic acetylcholine receptor has been identified in the rat genome. These genes are transcribed in the central and peripheral nervous systems in areas known to contain functional nicotinic receptors. In this paper, we demonstrate that three of these ...
Purified ? -toxin from Naja nigricollis snake venom labeled by [3H]acetylation binds specifically to the acetylcholine receptors of mouse neuroblastoma cells. Toxin binding was inhibited by inhibitors for nicotinic and muscarinic acetylcholine receptors. Clones of neuroblastoma cells were selected for low ...
The authors analyze the role of dopamine and muscarinic acetylcholine receptor blocking components in the antistereotypic action of neuroleptics with different chemical structure. To determine dopamine-blocking activity in vitro, binding of /sup 3/H-spiperone with membranes of the rat striatum was measured. To study the blocking action ...
Energy Citations Database
Fluorescence based screening assays are provided that allow for the for identification of agents that selectively bind to a ligand-gated ion channel (LGIC) such as a nicotinic acetylcholine receptor (nAChR). Also provided are methods for identifying agent...
National Technical Information Service (NTIS)
... Title : Primary Structure of Nicotinic Acetylcholine Receptor. ... One of the neurotransmitters used in the mammalian brain is acetylcholine. ...
Cellular/Molecular Galantamine Activates Muscle-Type Nicotinic Acetylcholine Receptors without activate the nicotinic ACh receptor or modulate its activation by ACh. In this study, we have examined the ability of galantamine to directly activate the ...
E-print Network
... This family includes Neu differentiation factor (NDF), heregulin (HRG), acetylcholine receptor inducing activity (ARIA), and the glial growth factors ...
... Accession Number : ADD754483. Title : ACETYLCHOLINE RECEPTOR CHANNEL IMAGED IN THE OPEN STATE. Descriptive ...
... Title : ACETYLCHOLINE RECEPTOR BINDING CHARACTERISTICS OF SNAKE AND CONE SNAIL VENOM POSTSYNAPTIC NEUROTOXINS ...
Partial contents include: Light-activated channels in limulus ventral photoreceptors; Paramagnetic hydrophobic ions as probes for electrically active conformational transitions in Ion channels; Acetylcholine receptor. Dynamic properties; Acetylcholine-activated channel current-voltage relations ...
To establish the functional modification produced by phosphorylation of the nicotinic acetylcholine receptor (AChR) single channel currents activated by acetylcholine (ACh) from purified Torpedo californica AChR reconstituted in lipid bilayers were record...
We are studying the properties of the active site, and the region peripheral to it, of acetylcholinesterase (AcChE), the enzyme which hydrolyzes and thus terminates the action of the important neurotransmitter acetylcholine (AcCh). We have studied the res...
The document is a review of research on the mechanisms which genetically regulate the compartmentalized expression of acetylcholine receptors (AchR) in adult vertebrate muscle tissue. It discusses studies of regulation through electrical activity, in part...
The nicotine receptor for the neurotransmitter acetylcholine is an allosteric protein composed of four different subunits assembled in a transmembrane pentamer ? 2? ? ? . The protein carries two acetylcholine sites at the level of the ? subunits and contains the ion channel. The complete sequence of the four subunits is known. The ...
Acetylcholine receptor, a component of the specialized muscle membrane, appears during the differentiation of embryonic myogenic cells in tissue culture. Demonstration of incorporation of the radioactive precursor L-[35S]methionine into purified receptor polypeptides is presented as evidence for its synthesis de novo. The identity of ...
Fourier transform infrared spectroscopy is used to characterize specific interactions between negatively charged lipids, such as phosphatidic acid, and the purified nicotinic acetylcholine receptor from Torpedo californica. The specific interaction of phosphatidic acid with acetylcholine receptor is demonstrated by ...
The structure of the open-channel form of the acetylcholine receptor has been determined from electron images of Torpedo ray postsynaptic membranes activated by brief (<5ms) mixing with droplets containing acetylcholine. Comparison with the closed-channel form shows that acetylcholine ...
Mar 1, 2011... induces the aggregation of nicotinic acetylcholine receptors on ... aggregation activity with as little as 5 ug secreted protein per ml ...
NASA Website
Cortical processing is strongly influenced by the actions of neuromodulators such as acetylcholine (ACh). Early studies in anaesthetized cats argued that acetylcholine can cause a sharpening of orientation tuning functions and an improvement of the signal-to-noise ratio (SNR) of neuronal responses in primary visual cortex (V1). Recent in vitro studies have ...
