... Accession Number : ADD754483. Title : ACETYLCHOLINE RECEPTOR CHANNEL IMAGED IN THE OPEN STATE. Descriptive ...
DTIC Science & Technology
... Title : The Ionic Channel of the Acetylcholine Receptor: Regulation by Sites Outside and Inside the Cell Membrane Which are Sensitive to ...
... Title : Activation, Inactivation, and Desensitization of Acetylcholine Receptor Channel Complex Detected by Binding of Perhydrohistrionicotoxin. ...
... Accession Number : ADA109246. Title : Nereistoxin Interaction with the Acetylcholine Receptor-Ionic Channel Complex. ...
... Accession Number : ADA109241. Title : Coupling between the Nicotinic Acetylcholine Receptor Site and the Ionic Channel Site. ...
... with the Ionic Channel of the Acetylcholine Receptor and Electrogenic Membrane. ... IONS, REPRINTS, MEMBRANES(BIOLOGY), BIOCHEMISTRY ...
... Title : Interaction of Imipramine with the Ionic Channel of the Acetylcholine Receptor of Motor Endplate and Electric Organ. ...
... TEST METHODS, CHEMICAL BONDS, MOLECULAR BIOLOGY, CHOLINESTERASE INHIBITORS, FROGS, PHARMACOLOGICAL ...
... mRNA LeveLs at Early and Late Time Points. ... T (1987) Acetylcholine receptors and sodium channels in ... in skeletal muscle by blockers of protein ...
... NICOTINIC ACETYLCHOLINE RECEPTOR ANNUAL REPORT MICHAEL A. RAFTERY ... 7 2. Exposure of acetylcholine receptor to the lipid bilayer 7 ...
The Torpedo and calf acetylcholine receptors and hybrids composed of subunits from the two species have been produced in Xenopus oocytes by the use of the cloned complementary DNAs. Single-channel current measurements indicate that these receptors form channels of similar conductance but with ...
NASA Astrophysics Data System (ADS)
... Title : Meproadifen Enhances Activitation and Desensitization of the Acetylcholine Receptor-Ionic Channel Complex (AChR): Single Channel ...
Antibody against acetylcholine receptor induces an increase in the rate of degradation of acetylcholine receptors on a mouse cell line (BC3H-1) and cultured rat skeletal muscle. The increased rate of degradation results in a lowered density of acetylcholine receptors on ...
for nicotinic acetylcholine receptors,22 TRPV1 channels transient receptor potential family of ion channels ,23
E-print Network
... CRECTR15 o IRREVERSIBLE ORGANOPHOSPHATE EFFECTS __ ON NICOTINIC ACETYLCHOLINE RECEPTOR/ION N CHANNEL COMPLEX ...
Partial contents include: Light-activated channels in limulus ventral photoreceptors; Paramagnetic hydrophobic ions as probes for electrically active conformational transitions in Ion channels; Acetylcholine receptor. Dynamic properties; Acetylcholine-activated channel ...
Energy Citations Database
Two kinetic models are introduced which predict amplitudes and time-courses of endplate currents and miniature endplate currents at neuromuscular junctions, at both normal and acetylcholinesterase-inhibited endplates. Appropriate differential rate equations reflecting interactions of acetylcholine with acetylcholine receptor and with ...
PubMed Central
To establish the functional modification produced by phosphorylation of the nicotinic acetylcholine receptor (AChR) single channel currents activated by acetylcholine (ACh) from purified Torpedo californica AChR reconstituted in lipid bilayers were record...
National Technical Information Service (NTIS)
... 1tmn neand are more Sbnsitive than the receptor Nites to treatment by the deter- gent Triton X-400 and suljhyaryl reducing and alkylating agents. ...
Fluorescence based screening assays are provided that allow for the for identification of agents that selectively bind to a ligand-gated ion channel (LGIC) such as a nicotinic acetylcholine receptor (nAChR). Also provided are methods for identifying agent...
The nicotine receptor for the neurotransmitter acetylcholine is an allosteric protein composed of four different subunits assembled in a transmembrane pentamer ? 2? ? ? . The protein carries two acetylcholine sites at the level of the ? subunits and contains the ion channel. The complete sequence of the four ...
The structure of the open-channel form of the acetylcholine receptor has been determined from electron images of Torpedo ray postsynaptic membranes activated by brief (<5ms) mixing with droplets containing acetylcholine. Comparison with the closed-channel form shows that ...
... Title : Turnover of Acetylcholine Receptors: Mechanisms of Regulation. ... Acetylcholine receptors, RA5 Neuromuscular junctions, Motor nerves. ...
The nicotinic acetylcholine receptor is a prototype ligand-gated ion channel that mediates signal transduction in the neuromuscular junctions and other cholinergic synapses. The molecular basis for the energetics of ligand binding and unbinding is critical to our understanding of the pharmacology of this class of ...
. In particular, we describe separate lines with human a7 and human a4b2 nicotinic acetylcholine receptors, mouse Introduction Nicotinic acetylcholine receptors (nAChRs) are pentameric ligand-gated ion channels (LGICAChR is targeted for schizophrenia [2], Alzheimer's disease [3], and cognition ...
Nicotinic acetylcholine receptors are pentameric ligand-gated ion channels, which are involved in a wide range of neuronal functions. During the past decade, a large number of nicotinic acetylcholine receptor subunits have been cloned and showed a discreet yet overlapping distribution pattern. ...
... Title : Primary Structure of Nicotinic Acetylcholine Receptor. ... One of the neurotransmitters used in the mammalian brain is acetylcholine. ...
... Title : ACETYLCHOLINE RECEPTOR BINDING CHARACTERISTICS OF SNAKE AND CONE SNAIL VENOM POSTSYNAPTIC NEUROTOXINS ...
Biophysical and Ion Channel Functional Characterization of the Torpedo californica Nicotinic Springer Science+Business Media, LLC 2008 Abstract The nicotinic acetylcholine receptor (nAChR) of Torpedo-008-9107-7 #12;2002). nAChRs from Torpedo electric rays are five-subunit integral membrane protein complexes
Nicotinic acetylcholine receptors are ligand-gated ion conducting transmembrane channels from the Cys-loop receptor super-family. The ?4?2 subtype is the predominant heteromeric subtype of nicotinic receptors found in the brain. Allosteric modulators for ?4?2 receptors ...
PubMed
Protein phosphorylation is considered a key event in synaptic physiology. The acetylcholine receptor (AChR), a prototype of ligand-gated ion channels, plays a central role in postsynaptic signal transduction. To examine the functional consequences of AChR...
The authors review three examples of how fluorescence spectroscopic techniques have been employed to elucidate structural features of the nicotinic acetylcholine receptor from the electric organ of Torpedo californica. The instrumentation and most relevant fluorescence methods utilized for these studies are briefly discussed. The examples reviewed are as ...
Fourier transform infrared spectroscopy is used to characterize specific interactions between negatively charged lipids, such as phosphatidic acid, and the purified nicotinic acetylcholine receptor from Torpedo californica. The specific interaction of phosphatidic acid with acetylcholine receptor is demonstrated by ...
The major brain nicotinic acetylcholine receptor is assembled from two subunits termed ? 4 and n? 1. When expressed in Xenopus oocytes, these subunits reconstitute a functional acetylcholine receptor that is inhibited by progesterone levels similar to those found in serum. In this report, we show that the steroid ...
Ionic conductance channels that are opened by activating nicotinic acetylcholine receptors at synapses of sympathetic neurons are permeable to small organic amines. Uptake of a tritium-labeled amine through these channels can be measured by autoradiography. This provides a simple and direct way to assess the ...
The nicotinic acetylcholine receptor (AcChR) is a complex of four polypeptides of molecular weight 40,000, 50,000, 60,000 and 65,000 daltons, generally referred to as alpha, Beta, gamma, sigma, which we first described (1) and this has since been confirme...
Quercetin mainly exists in the skin of colored fruits and vegetables as one of flavonoids. Recent studies show that quercetin, like other flavonoids, has diverse pharmacological actions. However, relatively little is known about quercetin effects in the regulations of ligand-gated ion channels. In the previous reports, we have shown that quercetin regulates subsets of ...
Acetylcholine reduced atrial contractions by 82.5% in guinea pig, 50.8% in rat, and 41.5% in rabbit. 2. The EC50, values for the negative inotropic effect of acetylcholine were 3.3 x 10(-7) M in rat and guinea pig atria and 4.1 x 10(-6) M in rabbit atria. 3. There was no correlation between the species differences in the negative inotropic effect of ...
and GABA receptors with anion-selective channels and serotonin 5HT3 receptors and nicotinic acetyl- choline protocols (6,7). Briefly, GLIC was expressed in the Escherichia coli, extracted with n. Time-resolved photo- labeling of the nicotinic acetylcholine receptor by [3 H]azietomidate, an open
