... mRNA LeveLs at Early and Late Time Points. ... T (1987) Acetylcholine receptors and sodium channels in ... in skeletal muscle by blockers of protein ...
DTIC Science & Technology
The Neuromuscular Disease Center at Washington University provides this comprehensive Web site for information on acetylcholine and acetylcholine receptors. Visitors will find detailed Web pages covering acetylcholine metabolism, acetylcholine receptors (including ...
NSDL National Science Digital Library
... The acetylcholine receptor protein is purified by affinity chromatogrpahy reincorporated into lipid vesicles of polymerized lipids, and mounted on a ...
A family of genes coding for proteins homologous to the ? subunit of the muscle nicotinic acetylcholine receptor has been identified in the rat genome. These genes are transcribed in the central and peripheral nervous systems in areas known to contain functional nicotinic receptors. In this paper, we demonstrate ...
NASA Astrophysics Data System (ADS)
Acetylcholine receptor phosphorylation has been compared in sealed and lysed right-side-out membrane vesicles prepared from Torpedo californica electric organ. Phosphorylation was increased 5- to 12-fold in hypotonically lysed vesicles as compared with untreated vesicles. Control experiments confirm that this enhancement is a result of increased ...
The nicotine receptor for the neurotransmitter acetylcholine is an allosteric protein composed of four different subunits assembled in a transmembrane pentamer ? 2? ? ? . The protein carries two acetylcholine sites at the level of the ? subunits and contains the ion channel. The complete ...
Fluorescence based screening assays are provided that allow for the for identification of agents that selectively bind to a ligand-gated ion channel (LGIC) such as a nicotinic acetylcholine receptor (nAChR). Also provided are methods for identifying agent...
National Technical Information Service (NTIS)
... Title : Turnover of Acetylcholine Receptors: Mechanisms of Regulation. ... Acetylcholine receptors, RA5 Neuromuscular junctions, Motor nerves. ...
Assembly and trafficking of nicotinic acetylcholine receptors (Review) NEIL S. MILLAR & PATRICIA C and in revised form 25 February 2008) Abstract Nicotinic acetylcholine receptors (nAChRs) are members. Nicotinic receptors are complex pentameric transmembrane proteins ...
E-print Network
All four subunits of the acetylcholine receptor in membrane vesicles isolated from Torpedo californica have been labeled with (/sup 3/H)cholesteryl diazoacetate. As this probe incorporates into lipid bilayers analogously to cholesterol, this result indicates that acetylcholine receptor interacts with cholesterol. ...
Energy Citations Database
The nicotinic acetylcholine receptor is a prototype ligand-gated ion channel that mediates signal transduction in the neuromuscular junctions and other cholinergic synapses. The molecular basis for the energetics of ligand binding and unbinding is critical to our understanding of the pharmacology of this class of receptors. Here we ...
PubMed Central
... Title : Primary Structure of Nicotinic Acetylcholine Receptor. ... One of the neurotransmitters used in the mammalian brain is acetylcholine. ...
... Accession Number : ADD754483. Title : ACETYLCHOLINE RECEPTOR CHANNEL IMAGED IN THE OPEN STATE. Descriptive ...
... Title : ACETYLCHOLINE RECEPTOR BINDING CHARACTERISTICS OF SNAKE AND CONE SNAIL VENOM POSTSYNAPTIC NEUROTOXINS ...
Muscarinic acetylcholine receptors are members of the G-protein coupled receptor superfamily that are expressed in and regulate the function of neurons, cardiac and smooth muscle, glands, and many other cell types and tissues. The correct trafficking of membrane proteins to the cell surface and ...
To establish the functional modification produced by phosphorylation of the nicotinic acetylcholine receptor (AChR) single channel currents activated by acetylcholine (ACh) from purified Torpedo californica AChR reconstituted in lipid bilayers were record...
This study evaluates the biochemical and genetic aspects of the acetylcholine receptor proteins and cholinergic ligands in Drosophila melanogaster and Torpedo californica. Included are (1) a comparative study of nicotinic ligand-induced cation release from acetylcholine receptors isolated from ...
Mar 1, 2011... induces the aggregation of nicotinic acetylcholine receptors on ... aggregation activity with as little as 5 ug secreted protein per ml ...
NASA Website
Fourier transform infrared spectroscopy is used to characterize specific interactions between negatively charged lipids, such as phosphatidic acid, and the purified nicotinic acetylcholine receptor from Torpedo californica. The specific interaction of phosphatidic acid with acetylcholine receptor is demonstrated by ...
Black lipid membranes were formed with oxidized cholesterol in the presence of either the acetylcholine receptor, purified from the electric organ of the electric ray Torpedo californica or its tryptic digest. In both cases, conductance of cations increased and was dependent on the concentration of the receptor ...
Seven serotypes of botulinum neurotoxin (BoNT) are a group of water- soluble large proteins that act on the presynaptic nerve cells of the neuro- muscular junctions. BoNTs act intracellulary to block acetylcholine neurotransmitter release leading to the f...
This study examined the regulation of G protein-coupled receptor kinase by G protein. Musucarinic acetylcholine receptor (mAChR) kinase purified from swine brains was proved to be able to regulate phosphorylation of muscarinic receptors by G protein; ...
... Title : Activation, Inactivation, and Desensitization of Acetylcholine Receptor Channel Complex Detected by Binding of Perhydrohistrionicotoxin. ...
The major brain nicotinic acetylcholine receptor is assembled from two subunits termed ? 4 and n? 1. When expressed in Xenopus oocytes, these subunits reconstitute a functional acetylcholine receptor that is inhibited by progesterone levels similar to those found in serum. In this report, we show that the steroid ...
