... is described that measures the sensitivity of acetylcholinesterase (AChE) activity to inhibition by organophosphorus (OP) insecticides in ... for resistant individuals. The technique will also measure ACh...
NBII National Biological Information Infrastructure
... used to detect insecticide resistance by measuring the activity levels of esterase (EST), acetylcholinesterase (AChE), altered AChE (insecticide ... population was not significantly different. Table 3 En...
... implications in relation to the evolution of insecticide resistance in this ... for research and training. Anthony, N., T. Rocheleau, G. Mocelin, H. J. ... ...
... Pyrethroids are the only compounds currently recommended for impregnation because of high insecticide activity, low mammalian toxicity, ... J. M. Hougard. Insecticide mixtures for mosquito net impregnatio...
... acetylcholinesterase ..... 9 4.2 Development of a test for insensitive AChE ..... 9 5. MALATHION-CARBOXYLESTERASE ..... 10 6 ...
DTIC Science & Technology
... this hypothesis by quantifying the energetic resources (lipids, glycogen, and glucose) of larvae and adult females of ... 31, 2010Keywords: life history trade-offs, carboxylesterase, acetylcholinesterase,...
BackgroundInsecticide resistance is now common in insects due to the frequent use of chemicals to control them, which provides a useful tool to study the adaptation of eukaryotic genome to new environments. Although numerous potential mutations may provide high level of resistance, only few alleles are found in insect natural ...
PubMed Central
BackgroundOrganophosphate and carbamate insecticides irreversibly inhibit acetylcholinesterase causing death of insects. Resistance-modified acetylcholinesterases(AChEs) have been described in many insect species and sequencing of their genes allowed several point mutations to be described. However, their relative ...
... References CitedAldridge, W. N. Some properties of specific cholinesterase with particular reference to the mechanism of inhibition ... trigonal carboxyl esters and tetrahedral alkylphosphonyl esters in c...
... electric eel and a range of insect acetylcholinesterases (AChE). Incubation of chlorpyrifos and sulprofos with meta-chloroperoxybenzoic ... MCPBA) produced active metabolites capable of distinguishing bet...
The extensive use of insecticides to control vector populations has lead to the widespread development of different mechanisms of insecticide resistance. Mutations that confer insecticide resistance are often associated to fitness costs that prevent them from spreading to fixation. In vectors, ...
PubMed
The penetration of 14C-labelled malathion was found to be similar in two bedbug strains, of which one was susceptible and the other moderately resistant to a range of organophosphorus insecticides. The latter strain was especially resistant to malathion and fenchlorphos, but was not resistant ...
Acetylcholinesterase (AChE) and its mutation recently emerged as a significant research area, due to its resistance against organophosphate and carbamate insecticides. Residue G265, which is always a conservative residue, mutated to A265 is the most frequent mutant of AChE in Drosophila populations. However, only this mutation caused a ...
Extensive utilization of pesticides against insects provides us with a good model for studying the adaptation of a eukaryotic genome to a strong selective pressure. One mechanism of resistance is the alteration of acetylcholinesterase (EC 3.1.1.7), the molecular target for organophosphates and carbamates. Here, we report the sequence analysis of the Ace ...
... the data presented here, the only 'biochemical lesion' produced by organophosphorous anticholinesterase compounds and insecticides that need ...
in the Mosquito Culex pipiens Pierrick Labbe�,* Arnaud Berthomieu,* Claire Berticat,* Haoues Alout,* Michel for the occurrence of several (at least 3) independent duplications of the ace-1 locus in the mosquito Culex pipiens resistance gene, duplication, mosquito, Culex pipiens, selection, ace-1. E-mail: weill@isem.univ-montp2.fr
E-print Network
The spread of resistance to pyrethroids in the major Afrotropical malaria vectors Anopheles gambiae s.s. necessitates the development of new strategies to control resistant mosquito populations. To test the efficacy of nets treated with repellent and insecticide against susceptible and insecticide-resistant An. ...
Gene duplication is thought to be the main potential source of material for the evolution of new gene functions. Several models have been proposed for the evolution of new functions through duplication, most based on ancient events (Myr). We provide molecular evidence for the occurrence of several (at least 3) independent duplications of the ace-1 locus in the mosquito Culex pipiens, selected in ...
The status of genes conferring resistance to organophosphate and carbamate insecticides has been examined in Culex pipiens pipiens mosquitoes sampled in Algeria. Presence of overproduced esterases was sporadic, but acetylcholinesterase-1 resistant alleles were observed in almost all samples. We focused our study on ...
BackgroundAcetylcholinesterase is irreversibly inhibited by organophosphate and carbamate insecticides allowing its use in biosensors for detection of these insecticides. Drosophila acetylcholinesterase is the most sensitive enzyme known and has been improved by in vitro mutagenesis. However, its stability has to ...
Insecticide resistance is one of the major threats to the effectiveness of vector control programs. In order to establish a baseline susceptibility profile of Aedes aegypti in the southwest of Colombia, 10 localities in four Departments (States) were evaluated. Standardized WHO bioassay, CDC bottle bioassay and microplate biochemical assays of non-specific ...
BackgroundIndoor residual spraying (IRS) has again become popular for malaria control in Africa. This combined with the affirmation by WHO that DDT is appropriate for use in the absence of longer lasting insecticide formulations in some malaria endemic settings, has resulted in an increase in IRS with DDT as a major malaria vector control intervention in Africa. DDT was ...
... susceptibility to atherosclerosis, and to pollutants and insecticides1. ... PON1 gene are highly susceptible to atherosclerosis and to OP poisoning3. ...
Simple microplate assay methods for determining the frequency of insecticide resistance in single mosquitos were used to study the distribution and localization of organophosphate and carbamate resistance in field populations of Anopheles albimanus Weidemann in Guatemala, where such resistance, caused by heavy use ...
An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four ...
BackgroundIndoor Residual Spraying (IRS), insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) are key components in malaria prevention and control strategy. However, the development of resistance by mosquitoes to insecticides recommended for IRS and/or ITNs/LLINs would affect ...
A high level of resistance to organophosphorus insecticides was recorded in a local population of the Colorado potato beetle (Ufa raion, Bashkortostan). Toxicological analysis demonstrated that the fraction of susceptible individuals did not exceed 0.025. DNA analysis of the polymorphism of acetylcholinesterase gene (AChE) according to ...
... Emergency Control Procedure for Insecticide-Resistant Body ... Descriptors : *LICE, *INSECT CONTROL, *INSECTICIDES, *PHOSPHINE, MILITARY ...
Invasive mosquitoes are economic and sanitary concerns especially in Europe and America. Most work has emphasized the role of resistance [Berrada, S., Fournier, D., Cuany, A., Nguyen, T.X., 1994. Identification of resistance mechanisms in a selected laboratory strain of Cacopsylla pyri (Homoptera: Psyllidae): altered ...
Tissue distribution of human acetyicholinesterase and butyryicholinesterase messenger RNA. 1 Cholinesterase inhibitors occur naturally in the calabar bean (eserine), green potatoes (solanine), insect-resistant crab apples, the coca plant (cocaine) and snake venom (fasciculin). There are also synthetic cholinesterase inhibitors, for example man-made ...
Energy Citations Database
In order to establish the insecticide susceptibility status for Anopheles darlingi in Colombia, and as part of the National Network on Insecticide Resistance Surveillance, five populations of insects from three Colombian states were evaluated. Standardised WHO and CDC bottle bioassays, in addition to microplate biochemical assays, were ...
Organophosphorus and carbamate insecticides are toxic to insects and mammals by virtue of their ability to inactivate the enzyme acetylcholinesterase. This review addresses the mechanism of inhibition of acetylcholinesterase by organophosphorus and carbamate esters, focusing on structural requirements necessary for anticholinesterase ...
BackgroundThe current status of insecticide resistance and the underlying resistance mechanisms were studied in the major vector of malaria, Anopheles culicifacies, and the secondary vector, Anopheles subpictus in five districts (Anuradhapura, Kurunegala, Moneragala, Puttalam and Trincomalee) of Sri Lanka. Eight other anophelines, ...
RESEARCH Open Access Insecticide resistance and malaria transmission: infection rate and oocyst by the spread of insecticide resistance. One factor that has been hitherto largely overlooked is the potential effects of insecticide resistance on the ability of mosquitoes to ...
