24 CFR 582.325 - Outreach activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMMUNITY FACILITIES SHELTER PLUS CARE Program Requirements § 582.325 Outreach...

77 FR 12071 - Agency Information Collection Activities: Form G-28, Revision of a Currently Approved Information...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-28

... Collection Activities: Form G-28, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form G- 28, Notice of Entry of Appearance... technology, e.g., permitting electronic submission of responses. Overview of This information collection: (1...

42 CFR 488.325 - Disclosure of results of surveys and activities.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 5 2014-10-01 2014-10-01 false Disclosure of results of surveys and activities... PROCEDURES Survey and Certification of Long-Term Care Facilities § 488.325 Disclosure of results of surveys... of imposed remedies. (5) Final appeal results. (6) Notice of termination of a facility. (7) Medicare...

42 CFR 488.325 - Disclosure of results of surveys and activities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 5 2011-10-01 2011-10-01 false Disclosure of results of surveys and activities... PROCEDURES Survey and Certification of Long-Term Care Facilities § 488.325 Disclosure of results of surveys... of imposed remedies. (5) Final appeal results. (6) Notice of termination of a facility. (7) Medicare...

42 CFR 488.325 - Disclosure of results of surveys and activities.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 5 2013-10-01 2013-10-01 false Disclosure of results of surveys and activities... PROCEDURES Survey and Certification of Long-Term Care Facilities § 488.325 Disclosure of results of surveys... of imposed remedies. (5) Final appeal results. (6) Notice of termination of a facility. (7) Medicare...

40 CFR 745.325 - Lead-based paint activities: State and Tribal program requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL ACT LEAD-BASED PAINT POISONING PREVENTION IN CERTAIN RESIDENTIAL STRUCTURES State and Indian Tribal Programs § 745.325 Lead-based paint activities: State and... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Lead-based paint activities: State and...

40 CFR 745.325 - Lead-based paint activities: State and Tribal program requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL ACT LEAD-BASED PAINT POISONING PREVENTION IN CERTAIN RESIDENTIAL STRUCTURES State and Indian Tribal Programs § 745.325 Lead-based paint activities: State and... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Lead-based paint activities: State and...

40 CFR 745.325 - Lead-based paint activities: State and Tribal program requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL ACT LEAD-BASED PAINT POISONING PREVENTION IN CERTAIN RESIDENTIAL STRUCTURES State and Indian Tribal Programs § 745.325 Lead-based paint activities: State and... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Lead-based paint activities: State and...

40 CFR 745.325 - Lead-based paint activities: State and Tribal program requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL ACT LEAD-BASED PAINT POISONING PREVENTION IN CERTAIN RESIDENTIAL STRUCTURES State and Indian Tribal Programs § 745.325 Lead-based paint activities: State and... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Lead-based paint activities: State and...

40 CFR 745.325 - Lead-based paint activities: State and Tribal program requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL ACT LEAD-BASED PAINT POISONING PREVENTION IN CERTAIN RESIDENTIAL STRUCTURES State and Indian Tribal Programs § 745.325 Lead-based paint activities: State and... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Lead-based paint activities: State and...

325 and 610 MHz radio counterparts of SNR G353.6-0.7 also known as HESS J1731-347

NASA Astrophysics Data System (ADS)

Nayana, A. J.; Chandra, Poonam; Roy, Subhashis; Green, David A.; Acero, Fabio; Lemoine-Goumard, Marianne; Marcowith, Alexandre; Ray, Alak K.; Renaud, Matthieu

2017-05-01

HESS J1731-347 also known as SNR G353.6-0.7 is one of the five known shell-type supernova remnants (SNRs) emitting in the very high energy (VHE, energy > 0.1 TeV) γ-ray domain. We observed this TeV SNR with the Giant Metrewave Radio Telescope (GMRT) in 1390, 610 and 325 MHz bands. In this paper, we report the discovery of 325 and 610 MHz radio counterparts of the SNR HESS J1731-347 with the GMRT. Various filaments of the SNR are clearly seen in the 325 and 610 MHz bands. However, the faintest feature in the radio bands corresponds to the peak in VHE emission. We explain this anti-correlation in terms of a possible leptonic origin of the observed VHE γ-ray emission. We determine the spectral indices of the bright individual filaments, which were detected in both the 610 and the 325 MHz bands. Our values range from -1.11 to -0.15, consistent with the non-thermal radio emission. We also report a possible radio counterpart of a nearby TeV source HESS J1729-345 from the 843 MHz Molonglo Galactic Plane Survey and the 1.4 GHz Southern Galactic Plane Survey maps. The positive radio spectral index of this possible counterpart suggests a thermal origin of the radio emission of this nearby TeV source.

The pretranslocation ribosome is targeted by GTP-bound EF-G in partially activated form

PubMed Central

Hauryliuk, Vasili; Mitkevich, Vladimir A.; Eliseeva, Natalia A.; Petrushanko, Irina Yu.; Ehrenberg, Måns; Makarov, Alexander A.

2008-01-01

Translocation of the tRNA·mRNA complex through the bacterial ribosome is driven by the multidomain guanosine triphosphatase elongation factor G (EF-G). We have used isothermal titration calorimetry to characterize the binding of GDP and GTP to free EF-G at 4°C, 20°C, and 37°C. The binding affinity of EF-G is higher to GDP than to GTP at 4°C, but lower at 37°C. The binding enthalpy and entropy change little with temperature in the case of GDP binding but change greatly in the case of GTP binding. These observations are compatible with a large decrease in the solvent-accessible hydrophobic surface area of EF-G on GTP, but not GDP, binding. The explanation we propose is the locking of the switch 1 and switch 2 peptide loops in the G domain of EF-G to the γ-phosphate of GTP. From these data, in conjunction with previously reported structural data on guanine nucleotide-bound EF-G, we suggest that EF-G enters the pretranslocation ribosome as an “activity chimera,” with the G domain activated by the presence of GTP but the overall factor conformation in the inactive form typical of a GDP-bound multidomain guanosine triphosphatase. We propose that the active overall conformation of EF-G is attained only in complex with the ribosome in its “ratcheted state,” with hybrid tRNA binding sites. PMID:18836081

ASASSN-18gq: Discovery of A Low-Luminosity Transient Towards Very Nearby ( 3.4 Mpc) Galaxy ESO 325- G?011

NASA Astrophysics Data System (ADS)

Nicholls, B.; Brimacombe, J.; Vallely, P.; Dong, Subo; Stanek, K. Z.; Kochanek, C. S.; Brown, J. S.; Shields, J.; Thompson, T. A.; Shappee, B. J.; Holoien, T. W.-S.; Prieto, J. L.; Bersier, D.; Bose, S.; Chen, Ping; Stritzinger, M.; Holmbo, S.

2018-04-01

During the ongoing All Sky Automated Survey for SuperNovae (ASAS-SN, Shappee et al. 2014), using data from the quadruple 14-cm "Payne-Gaposchkin" telescope in Sutherland, South Africa, and the quadruple 14-cm "Cassius" and "Paczynski" telescopes in Cerro Tololo, Chile, we discovered a new transient source, most likely a supernova, in the low surface brightness galaxy ESO 325- G?011.

17 CFR 249.325 - Form 13F, report of institutional investment manager pursuant to section 13(f) of the Securities...

Code of Federal Regulations, 2010 CFR

2010-04-01

... institutional investment manager pursuant to section 13(f) of the Securities Exchange Act of 1934. 249.325... manager pursuant to section 13(f) of the Securities Exchange Act of 1934. This form shall be used by institutional investment managers which are required to furnish reports pursuant to section 13(f) of the...

12 CFR 325.2 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Report.” (m) Leverage ratio means the ratio of Tier 1 capital to total assets, as calculated under this... assets may be included in calculating the bank's Tier 1 capital. (v) Tier 1 capital or core capital means... in excess of the limit set forth in § 325.5(g), minus identified losses (to the extent that Tier 1...

Evidence that antibiotic of Pantoea agglomerans E325 is produced and active against Erwinia amylovora on stigmas of pomaceous blossoms

USDA-ARS?s Scientific Manuscript database

Pantoea agglomerans strain E325, the active ingredient in a commercial product for fire blight, previously was shown to produce a unique pH-sensitive inhibitor in vitro that is specific to E. amylovora. To evaluate antibiosis as a mode of antagonism of E325, Tn5 mutagenesis was used to generate...

9 CFR 325.9 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false [Reserved] 325.9 Section 325.9 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION... CERTIFICATION TRANSPORTATION § 325.9 [Reserved] ...

40 CFR 421.325 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true [Reserved] 421.325 Section 421.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Secondary Uranium Subcategory § 421.325 [Reserved] ...

40 CFR 421.325 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true [Reserved] 421.325 Section 421.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Secondary Uranium Subcategory § 421.325 [Reserved] ...

40 CFR 421.325 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true [Reserved] 421.325 Section 421.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Secondary Uranium Subcategory § 421.325 [Reserved] ...

40 CFR 421.325 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false [Reserved] 421.325 Section 421.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Secondary Uranium Subcategory § 421.325 [Reserved] ...

40 CFR 421.325 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true [Reserved] 421.325 Section 421.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Secondary Uranium Subcategory § 421.325 [Reserved] ...

46 CFR 174.325 - Equalization.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Equalization. 174.325 Section 174.325 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SUBDIVISION AND STABILITY SPECIAL RULES PERTAINING TO SPECIFIC VESSEL TYPES Hopper Dredges With Working Freeboard Assignments Calculations § 174.325 Equalization...

46 CFR 174.325 - Equalization.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Equalization. 174.325 Section 174.325 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SUBDIVISION AND STABILITY SPECIAL RULES PERTAINING TO SPECIFIC VESSEL TYPES Hopper Dredges With Working Freeboard Assignments Calculations § 174.325 Equalization...

28 CFR 32.5 - Evidence.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Evidence. 32.5 Section 32.5 Judicial Administration DEPARTMENT OF JUSTICE PUBLIC SAFETY OFFICERS' DEATH, DISABILITY, AND EDUCATIONAL ASSISTANCE BENEFIT CLAIMS General Provisions § 32.5 Evidence. (a) Except as otherwise may be expressly provided in...

15 CFR 325.17 - Waiver.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Waiver. 325.17 Section 325.17 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES OF REVIEW § 325.17...

15 CFR 325.1 - Scope.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Scope. 325.1 Section 325.1 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES OF REVIEW § 325.1...

28 CFR 32.5 - Evidence.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Evidence. 32.5 Section 32.5 Judicial Administration DEPARTMENT OF JUSTICE PUBLIC SAFETY OFFICERS' DEATH, DISABILITY, AND EDUCATIONAL ASSISTANCE BENEFIT CLAIMS General Provisions § 32.5 Evidence. (a) Except as otherwise may be expressly provided in...

45 CFR 32.5 - Hearing.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 1 2012-10-01 2012-10-01 false Hearing. 32.5 Section 32.5 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION ADMINISTRATIVE WAGE GARNISHMENT § 32.5 Hearing. (a) In general. Upon timely written request of the debtor, the Secretary shall provide a hearing...

45 CFR 32.5 - Hearing.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Hearing. 32.5 Section 32.5 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION ADMINISTRATIVE WAGE GARNISHMENT § 32.5 Hearing. (a) In general. Upon timely written request of the debtor, the Secretary shall provide a hearing...

45 CFR 32.5 - Hearing.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 1 2014-10-01 2014-10-01 false Hearing. 32.5 Section 32.5 Public Welfare Department of Health and Human Services GENERAL ADMINISTRATION ADMINISTRATIVE WAGE GARNISHMENT § 32.5 Hearing. (a) In general. Upon timely written request of the debtor, the Secretary shall provide a hearing...

45 CFR 32.5 - Hearing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Hearing. 32.5 Section 32.5 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION ADMINISTRATIVE WAGE GARNISHMENT § 32.5 Hearing. (a) In general. Upon timely written request of the debtor, the Secretary shall provide a hearing...

TC-325 versus the conventional combined technique for endoscopic treatment of peptic ulcers with high-risk bleeding stigmata: A randomized pilot study.

PubMed

Kwek, Boon Eu Andrew; Ang, Tiing Leong; Ong, Peng Lan Jeannie; Tan, Yi Lyn Jessica; Ang, Shih Wen Daphne; Law, Ngai Moh; Thurairajah, Prem Harichander; Fock, Kwong Ming

2017-06-01

Preliminary studies on a new topical hemostatic agent, TC-325, have shown its safety and effectiveness in treating active upper gastrointestinal (GI) bleeding. However, to date there have been no randomized trials comparing TC-325 with the conventional combined technique (CCT). Our pilot study aimed to compare the efficacy and safety of TC-325 with those of CCT in treating peptic ulcers with active bleeding or high-risk stigmata. This was a comparative randomized study of patients with upper GI bleeding who had Forrest class I, IIA or IIB ulcers. Altogether 20 patients with a mean age of 70 years (range 23-87 years) were recruited, including 16 men, with a mean hemoglobin of 97 g/L. Initial hemostasis was successful in 19 (95.0%) patients, including 90.0% (9/10) in the TC-325 group and 100% (10/10) in the CCT group. TC-325 monotherapy failed to stop bleeding in a patient with Forrest IB posterior duodenal wall ulcer. Rebleeding was seen in 33.3% (3/9) of the patients in the TC-325 group and 10.0% (1/10) in the CCT group. One patient required angio-embolization therapy while three had successful conventional endotherapy. Two patients from the TC-325 group had serious adverse events that were not procedure- or therapy-related. In patients with Forrest IIA or IIB ulcers, five received TC-325 monotherapy; none had rebleeding. Our pilot study showed that TC-325 has a tendency towards a higher rebleeding rate than CCT, when treating actively bleeding ulcers. Larger trials are necessary for definitive results. © 2017 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and John Wiley & Sons Australia, Ltd.

G-Quadruplex Forming Oligonucleotides as Anti-HIV Agents.

PubMed

Musumeci, Domenica; Riccardi, Claudia; Montesarchio, Daniela

2015-09-22

Though a variety of different non-canonical nucleic acids conformations have been recognized, G-quadruplex structures are probably the structural motifs most commonly found within known oligonucleotide-based aptamers. This could be ascribed to several factors, as their large conformational diversity, marked responsiveness of their folding/unfolding processes to external stimuli, high structural compactness and chemo-enzymatic and thermodynamic stability. A number of G-quadruplex-forming oligonucleotides having relevant in vitro anti-HIV activity have been discovered in the last two decades through either SELEX or rational design approaches. Improved aptamers have been obtained by chemical modifications of natural oligonucleotides, as terminal conjugations with large hydrophobic groups, replacement of phosphodiester linkages with phosphorothioate bonds or other surrogates, insertion of base-modified monomers, etc. In turn, detailed structural studies have elucidated the peculiar architectures adopted by many G-quadruplex-based aptamers and provided insight into their mechanism of action. An overview of the state-of-the-art knowledge of the relevance of putative G-quadruplex forming sequences within the viral genome and of the most studied G-quadruplex-forming aptamers, selectively targeting HIV proteins, is here presented.

49 CFR 214.325 - Train coordination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Train coordination. 214.325 Section 214.325..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.325 Train coordination. Working limits established by a roadway worker through the use of train coordination shall comply with the...

49 CFR 214.325 - Train coordination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Train coordination. 214.325 Section 214.325..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.325 Train coordination. Working limits established by a roadway worker through the use of train coordination shall comply with the...

43 CFR 32.5 - Administrative requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false Administrative requirements. 32.5 Section 32.5 Public Lands: Interior Office of the Secretary of the Interior GRANTS TO STATES FOR ESTABLISHING YOUNG ADULT CONSERVATION CORPS (YACC) PROGRAM § 32.5 Administrative requirements. (a) The Governor in...

43 CFR 32.5 - Administrative requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Administrative requirements. 32.5 Section 32.5 Public Lands: Interior Office of the Secretary of the Interior GRANTS TO STATES FOR ESTABLISHING YOUNG ADULT CONSERVATION CORPS (YACC) PROGRAM § 32.5 Administrative requirements. (a) The Governor in...

49 CFR 325.91 - Exhaust systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Exhaust systems. 325.91 Section 325.91... EMISSION STANDARDS Exhaust Systems and Tires § 325.91 Exhaust systems. A motor vehicle does not conform to the visual exhaust system inspection requirements, 40 CFR 202.22, of the Interstate Motor Carrier...

49 CFR 325.91 - Exhaust systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 5 2012-10-01 2012-10-01 false Exhaust systems. 325.91 Section 325.91... EMISSION STANDARDS Exhaust Systems and Tires § 325.91 Exhaust systems. A motor vehicle does not conform to the visual exhaust system inspection requirements, 40 CFR 202.22, of the Interstate Motor Carrier...

49 CFR 325.91 - Exhaust systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Exhaust systems. 325.91 Section 325.91... EMISSION STANDARDS Exhaust Systems and Tires § 325.91 Exhaust systems. A motor vehicle does not conform to the visual exhaust system inspection requirements, 40 CFR 202.22, of the Interstate Motor Carrier...

47 CFR 22.325 - Control points.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 2 2013-10-01 2013-10-01 false Control points. 22.325 Section 22.325... Operational and Technical Requirements Operational Requirements § 22.325 Control points. Each station in the Public Mobile Services must have at least one control point and a person on duty who is responsible for...

47 CFR 22.325 - Control points.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false Control points. 22.325 Section 22.325... Operational and Technical Requirements Operational Requirements § 22.325 Control points. Each station in the Public Mobile Services must have at least one control point and a person on duty who is responsible for...

47 CFR 22.325 - Control points.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 2 2012-10-01 2012-10-01 false Control points. 22.325 Section 22.325... Operational and Technical Requirements Operational Requirements § 22.325 Control points. Each station in the Public Mobile Services must have at least one control point and a person on duty who is responsible for...

47 CFR 22.325 - Control points.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 2 2014-10-01 2014-10-01 false Control points. 22.325 Section 22.325... Operational and Technical Requirements Operational Requirements § 22.325 Control points. Each station in the Public Mobile Services must have at least one control point and a person on duty who is responsible for...

47 CFR 22.325 - Control points.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 2 2011-10-01 2011-10-01 false Control points. 22.325 Section 22.325... Operational and Technical Requirements Operational Requirements § 22.325 Control points. Each station in the Public Mobile Services must have at least one control point and a person on duty who is responsible for...

14 CFR 325.6 - Periodic reviews.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Periodic reviews. 325.6 Section 325.6... REGULATIONS ESSENTIAL AIR SERVICE PROCEDURES § 325.6 Periodic reviews. (a) The Department will start a periodic review of essential air service within 1 year of the date of the previous determination of...

45 CFR 150.325 - Settlement authority.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Settlement authority. 150.325 Section 150.325 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH CARE ACCESS CMS... Governmental Plans-Civil Money Penalties § 150.325 Settlement authority. Nothing in §§ 150.315 through 150.323...

49 CFR 325.91 - Exhaust systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 5 2010-10-01 2010-10-01 false Exhaust systems. 325.91 Section 325.91... EMISSION STANDARDS Exhaust Systems and Tires § 325.91 Exhaust systems. Link to an amendment published at 75 FR 57193, Sept. 20, 2010. A motor vehicle does not conform to the visual exhaust system inspection...

30 CFR 75.325 - Air quantity.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Air quantity. 75.325 Section 75.325 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.325 Air quantity. (a)(1) In bituminous and lignite mines the quantity of air shall be at least 3,000 cubic feet per minute reaching each working face...

30 CFR 75.325 - Air quantity.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Air quantity. 75.325 Section 75.325 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.325 Air quantity. (a)(1) In bituminous and lignite mines the quantity of air shall be at least 3,000 cubic feet per minute reaching each working face...

30 CFR 75.325 - Air quantity.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Air quantity. 75.325 Section 75.325 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.325 Air quantity. (a)(1) In bituminous and lignite mines the quantity of air shall be at least 3,000 cubic feet per minute reaching each working face...

30 CFR 75.325 - Air quantity.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Air quantity. 75.325 Section 75.325 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.325 Air quantity. (a)(1) In bituminous and lignite mines the quantity of air shall be at least 3,000 cubic feet per minute reaching each working face...

30 CFR 75.325 - Air quantity.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Air quantity. 75.325 Section 75.325 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.325 Air quantity. (a)(1) In bituminous and lignite mines the quantity of air shall be at least 3,000 cubic feet per minute reaching each working face...

7 CFR 4274.325 - Interest rates.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Interest rates. 4274.325 Section 4274.325 Agriculture... (IRP) § 4274.325 Interest rates. (a) Loans made by the Agency pursuant to this subpart shall bear interest at a fixed rate of 1 percent per annum over the term of the loan. (b) Interest rates charged by...

7 CFR 4274.325 - Interest rates.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 15 2011-01-01 2011-01-01 false Interest rates. 4274.325 Section 4274.325 Agriculture... (IRP) § 4274.325 Interest rates. (a) Loans made by the Agency pursuant to this subpart shall bear interest at a fixed rate of 1 percent per annum over the term of the loan. (b) Interest rates charged by...

7 CFR 4274.325 - Interest rates.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 15 2013-01-01 2013-01-01 false Interest rates. 4274.325 Section 4274.325 Agriculture... (IRP) § 4274.325 Interest rates. (a) Loans made by the Agency pursuant to this subpart shall bear interest at a fixed rate of 1 percent per annum over the term of the loan. (b) Interest rates charged by...

7 CFR 4274.325 - Interest rates.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 15 2014-01-01 2014-01-01 false Interest rates. 4274.325 Section 4274.325 Agriculture... (IRP) § 4274.325 Interest rates. (a) Loans made by the Agency pursuant to this subpart shall bear interest at a fixed rate of 1 percent per annum over the term of the loan. (b) Interest rates charged by...

7 CFR 4274.325 - Interest rates.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 15 2012-01-01 2012-01-01 false Interest rates. 4274.325 Section 4274.325 Agriculture... (IRP) § 4274.325 Interest rates. (a) Loans made by the Agency pursuant to this subpart shall bear interest at a fixed rate of 1 percent per annum over the term of the loan. (b) Interest rates charged by...

75 FR 10025 - Proposed Collection; Comment Request for Forms 8038, 8038-G, and 8038-GC

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-04

... 8038, 8038-G, and 8038-GC AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request... comments concerning Form 8038, Information Return for Tax-Exempt Private Activity Bond Issues, Form 8038-G... Bond Issues (Form 8038), Information Return for Tax-Exempt Governmental Obligation (Form 8038-G), and...

45 CFR 3.25 - Servicing of vehicles.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 1 2012-10-01 2012-10-01 false Servicing of vehicles. 3.25 Section 3.25 Public... THE NATIONAL INSTITUTES OF HEALTH FEDERAL ENCLAVE Traffic Regulations § 3.25 Servicing of vehicles. A... vehicle. ...

15 CFR 325.2 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Definitions. 325.2 Section 325.2 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES OF...

7 CFR 1210.325 - Procedure.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 10 2011-01-01 2011-01-01 false Procedure. 1210.325 Section 1210.325 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... mail, telegraph, telephone, or by other means of communication; Provided, That each member receives an...

7 CFR 1210.325 - Procedure.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Procedure. 1210.325 Section 1210.325 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... mail, telegraph, telephone, or by other means of communication; Provided, That each member receives an...

12 CFR 325.5 - Miscellaneous.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Miscellaneous. 325.5 Section 325.5 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY CAPITAL..., covenants, terms, restrictions, or provisions that are inconsistent with safe and sound banking practices. A...

17 CFR 32.5 - Disclosure.

Code of Federal Regulations, 2010 CFR

2010-04-01

... effect of any foreign currency fluctuations with respect to commodity option transactions which are to be... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Disclosure. 32.5 Section 32.5 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION REGULATION OF COMMODITY OPTION...

49 CFR 325.7 - Allowable noise levels.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Allowable noise levels. 325.7 Section 325.7... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL REGULATIONS COMPLIANCE WITH INTERSTATE MOTOR CARRIER NOISE EMISSION STANDARDS General Provisions § 325.7 Allowable noise levels. Motor vehicle noise emissions, when...

14 CFR 152.325 - Financial status report: Airport planning.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Financial status report: Airport planning... agency conducting a project for airport system planning shall submit a financial status report on a form... TRANSPORTATION (CONTINUED) AIRPORTS AIRPORT AID PROGRAM Accounting and Reporting Requirements § 152.325 Financial...

47 CFR 27.322-27.325 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false [Reserved] 27.322-27.325 Section 27.322-27.325 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS COMMUNICATIONS SERVICES Application, Licensing, and Processing Rules for WCS §§ 27.322-27.325 [Reserved] ...

47 CFR 27.322-27.325 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 2 2011-10-01 2011-10-01 false [Reserved] 27.322-27.325 Section 27.322-27.325 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS COMMUNICATIONS SERVICES Application, Licensing, and Processing Rules for WCS §§ 27.322-27.325 [Reserved] ...

33 CFR 117.325 - St. Johns River.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false St. Johns River. 117.325 Section 117.325 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.325 St. Johns River. (a) The drawspan...

2 CFR 170.325 - Subaward.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 2 Grants and Agreements 1 2013-01-01 2013-01-01 false Subaward. 170.325 Section 170.325 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GOVERNMENTWIDE GUIDANCE FOR GRANTS AND AGREEMENTS NATIONAL POLICY REQUIREMENTS REPORTING SUBAWARD AND EXECUTIVE...

2 CFR 170.325 - Subaward.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false Subaward. 170.325 Section 170.325 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GOVERNMENTWIDE GUIDANCE FOR GRANTS AND AGREEMENTS Reserved REPORTING SUBAWARD AND EXECUTIVE COMPENSATION...

2 CFR 170.325 - Subaward.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 2 Grants and Agreements 1 2011-01-01 2011-01-01 false Subaward. 170.325 Section 170.325 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GOVERNMENTWIDE GUIDANCE FOR GRANTS AND AGREEMENTS [Reserved] REPORTING SUBAWARD AND EXECUTIVE COMPENSATION...

6 CFR 27.325 - Burden of proof.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 6 Domestic Security 1 2010-01-01 2010-01-01 false Burden of proof. 27.325 Section 27.325 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY CHEMICAL FACILITY ANTI-TERRORISM STANDARDS Orders and Adjudications § 27.325 Burden of proof. The Assistant Secretary bears the initial...

6 CFR 27.325 - Burden of proof.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 6 Domestic Security 1 2011-01-01 2011-01-01 false Burden of proof. 27.325 Section 27.325 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY CHEMICAL FACILITY ANTI-TERRORISM STANDARDS Orders and Adjudications § 27.325 Burden of proof. The Assistant Secretary bears the initial...

6 CFR 27.325 - Burden of proof.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 6 Domestic Security 1 2013-01-01 2013-01-01 false Burden of proof. 27.325 Section 27.325 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY CHEMICAL FACILITY ANTI-TERRORISM STANDARDS Orders and Adjudications § 27.325 Burden of proof. The Assistant Secretary bears the initial...

6 CFR 27.325 - Burden of proof.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 6 Domestic Security 1 2014-01-01 2014-01-01 false Burden of proof. 27.325 Section 27.325 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY CHEMICAL FACILITY ANTI-TERRORISM STANDARDS Orders and Adjudications § 27.325 Burden of proof. The Assistant Secretary bears the initial...

6 CFR 27.325 - Burden of proof.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 6 Domestic Security 1 2012-01-01 2012-01-01 false Burden of proof. 27.325 Section 27.325 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY CHEMICAL FACILITY ANTI-TERRORISM STANDARDS Orders and Adjudications § 27.325 Burden of proof. The Assistant Secretary bears the initial...

20 CFR 325.5 - Death of employee.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Death of employee. 325.5 Section 325.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.5 Death of employee. If an employee dies before filing...

20 CFR 325.5 - Death of employee.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true Death of employee. 325.5 Section 325.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.5 Death of employee. If an employee dies before filing...

20 CFR 325.5 - Death of employee.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false Death of employee. 325.5 Section 325.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.5 Death of employee. If an employee dies before filing...

20 CFR 325.5 - Death of employee.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false Death of employee. 325.5 Section 325.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.5 Death of employee. If an employee dies before filing...

20 CFR 325.5 - Death of employee.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true Death of employee. 325.5 Section 325.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.5 Death of employee. If an employee dies before filing...

45 CFR 3.25 - Servicing of vehicles.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 1 2014-10-01 2014-10-01 false Servicing of vehicles. 3.25 Section 3.25 Public Welfare Department of Health and Human Services GENERAL ADMINISTRATION CONDUCT OF PERSONS AND TRAFFIC ON THE NATIONAL INSTITUTES OF HEALTH FEDERAL ENCLAVE Traffic Regulations § 3.25 Servicing of vehicles. A...

45 CFR 3.25 - Servicing of vehicles.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 1 2013-10-01 2013-10-01 false Servicing of vehicles. 3.25 Section 3.25 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CONDUCT OF PERSONS AND TRAFFIC ON THE NATIONAL INSTITUTES OF HEALTH FEDERAL ENCLAVE Traffic Regulations § 3.25 Servicing of vehicles. A...

45 CFR 3.25 - Servicing of vehicles.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Servicing of vehicles. 3.25 Section 3.25 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CONDUCT OF PERSONS AND TRAFFIC ON THE NATIONAL INSTITUTES OF HEALTH FEDERAL ENCLAVE Traffic Regulations § 3.25 Servicing of vehicles. A...

45 CFR 3.25 - Servicing of vehicles.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Servicing of vehicles. 3.25 Section 3.25 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CONDUCT OF PERSONS AND TRAFFIC ON THE NATIONAL INSTITUTES OF HEALTH FEDERAL ENCLAVE Traffic Regulations § 3.25 Servicing of vehicles. A...

15 CFR 325.13 - Nonadmissibility in evidence.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Nonadmissibility in evidence. 325.13 Section 325.13 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued... OF REVIEW § 325.13 Nonadmissibility in evidence. If the Secretary denies, in whole or in part, an...

15 CFR 325.14 - Submitting reports.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Submitting reports. 325.14 Section 325.14 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES...

20 CFR 325.4 - Claim for unemployment benefits.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Claim for unemployment benefits. 325.4 Section 325.4 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.4 Claim for unemployment benefits. (a...

20 CFR 325.4 - Claim for unemployment benefits.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false Claim for unemployment benefits. 325.4 Section 325.4 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.4 Claim for unemployment benefits. (a...

20 CFR 325.4 - Claim for unemployment benefits.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false Claim for unemployment benefits. 325.4 Section 325.4 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.4 Claim for unemployment benefits. (a...

20 CFR 325.4 - Claim for unemployment benefits.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true Claim for unemployment benefits. 325.4 Section 325.4 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.4 Claim for unemployment benefits. (a...

20 CFR 325.4 - Claim for unemployment benefits.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true Claim for unemployment benefits. 325.4 Section 325.4 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.4 Claim for unemployment benefits. (a...

49 CFR 325.9 - Measurement tolerances.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 5 2010-10-01 2010-10-01 false Measurement tolerances. 325.9 Section 325.9 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY... allowed to take into account the effects of the following factors: (1) The consensus standard practice of...

15 CFR 325.11 - Judicial review.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Judicial review. 325.11 Section 325.11 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES OF...

10 CFR 205.325 - Annual report.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 3 2011-01-01 2011-01-01 false Annual report. 205.325 Section 205.325 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS Electric Power System Permits and Reports... Construction, Connection, Operation, and Maintenance of Facilities for Transmission of Electric Energy at...

15 CFR 325.16 - Protecting confidentiality of information.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Protecting confidentiality of information. 325.16 Section 325.16 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign... EXPORT TRADE CERTIFICATES OF REVIEW § 325.16 Protecting confidentiality of information. (a) Any...

15 CFR 325.16 - Protecting confidentiality of information.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Protecting confidentiality of information. 325.16 Section 325.16 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign... EXPORT TRADE CERTIFICATES OF REVIEW § 325.16 Protecting confidentiality of information. (a) Any...

15 CFR 325.16 - Protecting confidentiality of information.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Protecting confidentiality of information. 325.16 Section 325.16 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign... EXPORT TRADE CERTIFICATES OF REVIEW § 325.16 Protecting confidentiality of information. (a) Any...

15 CFR 325.16 - Protecting confidentiality of information.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Protecting confidentiality of information. 325.16 Section 325.16 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign... EXPORT TRADE CERTIFICATES OF REVIEW § 325.16 Protecting confidentiality of information. (a) Any...

12 CFR 325.102 - Notice of capital category.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Notice of capital category. 325.102 Section 325.102 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY CAPITAL MAINTENANCE Prompt Corrective Action § 325.102 Notice of capital category. (a) Effective...

34 CFR 32.5 - Pre-offset hearing-general.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 1 2010-07-01 2010-07-01 false Pre-offset hearing-general. 32.5 Section 32.5 Education Office of the Secretary, Department of Education SALARY OFFSET TO RECOVER OVERPAYMENTS OF PAY OR ALLOWANCES FROM DEPARTMENT OF EDUCATION EMPLOYEES § 32.5 Pre-offset hearing—general. (a) An employee who...

42 CFR 426. 325 - What may be challenged.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 3 2012-10-01 2012-10-01 false What may be challenged. 426. 325 Section 426. 325 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... DETERMINATIONS General Provisions for the Review of LCDs and NCDs § 426. 325 What may be challenged. (a) Only...

42 CFR 426. 325 - What may be challenged.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 3 2013-10-01 2013-10-01 false What may be challenged. 426. 325 Section 426. 325 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... DETERMINATIONS General Provisions for the Review of LCDs and NCDs § 426. 325 What may be challenged. (a) Only...

42 CFR 426. 325 - What may be challenged.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 3 2010-10-01 2010-10-01 false What may be challenged. 426. 325 Section 426. 325 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... General Provisions for the Review of LCDs and NCDs § 426. 325 What may be challenged. (a) Only LCDs or...

42 CFR 426. 325 - What may be challenged.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 3 2011-10-01 2011-10-01 false What may be challenged. 426. 325 Section 426. 325 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... General Provisions for the Review of LCDs and NCDs § 426. 325 What may be challenged. (a) Only LCDs or...

29 CFR 780.325 - Principally engaged.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Agriculture That Is Exempted From the Minimum Wage and Overtime Pay Requirements Under Section 13(a)(6... 29 Labor 3 2010-07-01 2010-07-01 false Principally engaged. 780.325 Section 780.325 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR STATEMENTS OF GENERAL...

Human IgG is produced in a pro-form that requires clipping of C-terminal lysines for maximal complement activation

PubMed Central

van den Bremer, Ewald TJ; Beurskens, Frank J; Voorhorst, Marleen; Engelberts, Patrick J; de Jong, Rob N; van der Boom, Burt G; Cook, Erika M; Lindorfer, Margaret A; Taylor, Ronald P; van Berkel, Patrick HC; Parren, Paul WHI

2015-01-01

Human IgG is produced with C-terminal lysines that are cleaved off in circulation. The function of this modification was unknown and generally thought not to affect antibody function. We recently reported that efficient C1q binding and complement-dependent cytotoxicity (CDC) requires IgG hexamerization at the cell surface. Here we demonstrate that C-terminal lysines may interfere with this process, leading to suboptimal C1q binding and CDC of cells opsonized with C-terminal lysine-containing IgG. After we removed these lysines with a carboxypeptidase, maximal complement activation was observed. Interestingly, IgG1 mutants containing either a negative C-terminal charge or multiple positive charges lost CDC almost completely; however, CDC was fully restored by mixing C-terminal mutants of opposite charge. Our data indicate a novel post-translational control mechanism of human IgG: human IgG molecules are produced in a pro-form in which charged C-termini interfere with IgG hexamer formation, C1q binding and CDC. To allow maximal complement activation, C-terminal lysine processing is required to release the antibody's full cytotoxic potential. PMID:26037225

77 FR 21105 - Agency Information Collection Activities: Form G-1145, Revision of a Currently Approved...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-09

... and/or text message on their cell phone that their application or petition has been accepted, they are requested to provide their email address and/or cell phone number on Form G-1145, and attach the form to the...

40 CFR 1065.325 - Intake-flow calibration.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Section 1065.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS ENGINE-TESTING PROCEDURES Calibrations and Verifications Flow-Related Measurements § 1065.325 Intake-flow calibration. (a) Calibrate intake-air flow meters upon initial installation. Follow the...

Yeast Sub1 and human PC4 are G-quadruplex binding proteins that suppress genome instability at co-transcriptionally formed G4 DNA.

PubMed

Lopez, Christopher R; Singh, Shivani; Hambarde, Shashank; Griffin, Wezley C; Gao, Jun; Chib, Shubeena; Yu, Yang; Ira, Grzegorz; Raney, Kevin D; Kim, Nayun

2017-06-02

G-quadruplex or G4 DNA is a non-B secondary DNA structure consisting of a stacked array of guanine-quartets that can disrupt critical cellular functions such as replication and transcription. When sequences that can adopt Non-B structures including G4 DNA are located within actively transcribed genes, the reshaping of DNA topology necessary for transcription process stimulates secondary structure-formation thereby amplifying the potential for genome instability. Using a reporter assay designed to study G4-induced recombination in the context of an actively transcribed locus in Saccharomyces cerevisiae, we tested whether co-transcriptional activator Sub1, recently identified as a G4-binding factor, contributes to genome maintenance at G4-forming sequences. Our data indicate that, upon Sub1-disruption, genome instability linked to co-transcriptionally formed G4 DNA in Top1-deficient cells is significantly augmented and that its highly conserved DNA binding domain or the human homolog PC4 is sufficient to suppress G4-associated genome instability. We also show that Sub1 interacts specifically with co-transcriptionally formed G4 DNA in vivo and that yeast cells become highly sensitivity to G4-stabilizing chemical ligands by the loss of Sub1. Finally, we demonstrate the physical and genetic interaction of Sub1 with the G4-resolving helicase Pif1, suggesting a possible mechanism by which Sub1 suppresses instability at G4 DNA. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

40 CFR 89.325 - Engine intake air temperature measurement.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Engine intake air temperature measurement. 89.325 Section 89.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... Test Equipment Provisions § 89.325 Engine intake air temperature measurement. (a) Engine intake air...

Drain the lysosome: Development of the novel orally available autophagy inhibitor ROC-325.

PubMed

Carew, Jennifer S; Nawrocki, Steffan T

2017-04-03

Although macroautophagy/autophagy is a key contributor to malignant pathogenesis and therapeutic resistance, there are few FDA-approved agents that significantly affect this pathway. We used medicinal chemistry strategies to develop ROC-325, an orally available novel inhibitor of lysosomal-mediated autophagy. Detailed in vitro and in vivo studies in preclinical models of renal cell carcinoma demonstrated that ROC-325 triggered the hallmark features of lysosomal autophagy inhibition, was very well tolerated, and exhibited significant superiority with respect to autophagy inhibition and anticancer activity over hydroxychloroquine. Our findings support the clinical investigation of the safety and preliminary efficacy of ROC-325 in patients with autophagy-dependent malignancies and other disorders where aberrant autophagy contributes to disease pathogenesis.

31 CFR 560.325 - Property; property interest.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance:Treasury 3 2013-07-01 2013-07-01 false Property; property interest. 560.325 Section 560.325 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE..., or interest or interests therein, present, future, or contingent. ...

31 CFR 560.325 - Property; property interest.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance:Treasury 3 2014-07-01 2014-07-01 false Property; property interest. 560.325 Section 560.325 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE..., or interest or interests therein, present, future, or contingent. ...

24 CFR 1006.325 - Maintenance, management and efficient operation.

Code of Federal Regulations, 2010 CFR

2010-04-01

... § 1006.325 Maintenance, management and efficient operation. (a) Written policies. The DHHL must develop... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Maintenance, management and efficient operation. 1006.325 Section 1006.325 Housing and Urban Development Regulations Relating to Housing...

12 CFR 611.325 - Bank and association nominating committees.

Code of Federal Regulations, 2011 CFR

2011-01-01

....325 Section 611.325 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM ORGANIZATION Election of Directors and Other Voting Procedures § 611.325 Bank and association nominating committees... institution under § 611.320 and this section. (a) Composition. The voting stockholders of each bank and...

15 CFR 325.15 - Relinquishing a certificate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Relinquishing a certificate. 325.15 Section 325.15 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES...

15 CFR 325.4 - Calculating time periods.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Calculating time periods. 325.4 Section 325.4 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES...

15 CFR 325.7 - Amending the certificate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Amending the certificate. 325.7 Section 325.7 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES...

15 CFR 325.5 - Issuing the certificate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Issuing the certificate. 325.5 Section 325.5 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE CERTIFICATES...

49 CFR 325.75 - Ground surface correction factors. 1

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 5 2010-10-01 2010-10-01 false Ground surface correction factors. 1 325.75... MOTOR CARRIER NOISE EMISSION STANDARDS Correction Factors § 325.75 Ground surface correction factors. 1... account both the distance correction factors contained in § 325.73 and the ground surface correction...

49 CFR 325.75 - Ground surface correction factors. 1

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Ground surface correction factors. 1 325.75... MOTOR CARRIER NOISE EMISSION STANDARDS Correction Factors § 325.75 Ground surface correction factors. 1... account both the distance correction factors contained in § 325.73 and the ground surface correction...

33 CFR 325.5 - Forms of permits.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) DA permits under this regulation will be in the form of individual permits or general permits. The basic format shall be ENG Form 1721, DA Permit (Appendix A). (2) The general conditions included in ENG... be required. (2) Nationwide permits. Nationwide permits are a type of general permit and represent DA...

14 CFR 21.325 - Export airworthiness approvals.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Export airworthiness approvals. 21.325... AIRCRAFT CERTIFICATION PROCEDURES FOR PRODUCTS AND PARTS Export Airworthiness Approvals § 21.325 Export airworthiness approvals. (a) Kinds of approvals. (1) Export airworthiness approval of Class I products is issued...

2 CFR 25.325 - For-profit organization.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 2 Grants and Agreements 1 2012-01-01 2012-01-01 false For-profit organization. 25.325 Section 25.325 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GOVERNMENTWIDE GUIDANCE FOR GRANTS AND AGREEMENTS PRE-AWARD RESPONSIBILITIES UNIVERSAL...

2 CFR 25.325 - For-profit organization.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false For-profit organization. 25.325 Section 25.325 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GOVERNMENTWIDE GUIDANCE FOR GRANTS AND AGREEMENTS Reserved UNIVERSAL IDENTIFIER AND...

2 CFR 25.325 - For-profit organization.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 2 Grants and Agreements 1 2013-01-01 2013-01-01 false For-profit organization. 25.325 Section 25.325 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GOVERNMENTWIDE GUIDANCE FOR GRANTS AND AGREEMENTS PRE-AWARD RESPONSIBILITIES UNIVERSAL...

31 CFR 515.325 - National securities exchange.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false National securities exchange. 515.325... Definitions § 515.325 National securities exchange. The term national securities exchange shall mean an exchange registered as a national securities exchange under section 6 of the Securities Exchange Act of...

31 CFR 500.325 - National securities exchange.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false National securities exchange. 500.325... Definitions § 500.325 National securities exchange. The term national securities exchange shall mean an exchange registered as a national securities exchange under section 6 of the Securities Exchange Act of...

2 CFR 25.325 - For-profit organization.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 2 Grants and Agreements 1 2011-01-01 2011-01-01 false For-profit organization. 25.325 Section 25.325 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GOVERNMENTWIDE GUIDANCE FOR GRANTS AND AGREEMENTS Pre-award responsibilities UNIVERSAL...

14 CFR 139.325 - Airport emergency plan.

Code of Federal Regulations, 2011 CFR

2011-01-01

... response for the largest air carrier aircraft in the Index group required under § 139.315. (b) The plan... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Airport emergency plan. 139.325 Section 139.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED...

14 CFR 139.325 - Airport emergency plan.

Code of Federal Regulations, 2014 CFR

2014-01-01

... response for the largest air carrier aircraft in the Index group required under § 139.315. (b) The plan... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Airport emergency plan. 139.325 Section 139.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED...

14 CFR 139.325 - Airport emergency plan.

Code of Federal Regulations, 2013 CFR

2013-01-01

... response for the largest air carrier aircraft in the Index group required under § 139.315. (b) The plan... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Airport emergency plan. 139.325 Section 139.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED...

49 CFR 172.325 - Elevated temperature materials.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 2 2013-10-01 2013-10-01 false Elevated temperature materials. 172.325 Section... REQUIREMENTS, AND SECURITY PLANS Marking § 172.325 Elevated temperature materials. (a) Except as provided in paragraph (b) of this section, a bulk packaging containing an elevated temperature material must be marked...

49 CFR 172.325 - Elevated temperature materials.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 2 2012-10-01 2012-10-01 false Elevated temperature materials. 172.325 Section... REQUIREMENTS, AND SECURITY PLANS Marking § 172.325 Elevated temperature materials. (a) Except as provided in paragraph (b) of this section, a bulk packaging containing an elevated temperature material must be marked...

12 CFR 325.104 - Capital restoration plans.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Capital restoration plans. 325.104 Section 325.104 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL... filing plan—(1) In general. A bank shall file a written capital restoration plan with the appropriate...

49 CFR Appendix G to Part 40 - Alcohol Testing Form

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 1 2012-10-01 2012-10-01 false Alcohol Testing Form G Appendix G to Part 40 Transportation Office of the Secretary of Transportation PROCEDURES FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Pt. 40, App. G Appendix G to Part 40—Alcohol Testing Form The following form is the...

49 CFR Appendix G to Part 40 - Alcohol Testing Form

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 1 2011-10-01 2011-10-01 false Alcohol Testing Form G Appendix G to Part 40 Transportation Office of the Secretary of Transportation PROCEDURES FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Pt. 40, App. G Appendix G to Part 40—Alcohol Testing Form The following form is the...

49 CFR Appendix G to Part 40 - Alcohol Testing Form

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 1 2013-10-01 2013-10-01 false Alcohol Testing Form G Appendix G to Part 40 Transportation Office of the Secretary of Transportation PROCEDURES FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Pt. 40, App. G Appendix G to Part 40—Alcohol Testing Form The following form is the...

49 CFR Appendix G to Part 40 - Alcohol Testing Form

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 1 2014-10-01 2014-10-01 false Alcohol Testing Form G Appendix G to Part 40 Transportation Office of the Secretary of Transportation PROCEDURES FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Pt. 40, App. G Appendix G to Part 40—Alcohol Testing Form The following form is the...

49 CFR Appendix G to Part 40 - Alcohol Testing Form

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Alcohol Testing Form G Appendix G to Part 40 Transportation Office of the Secretary of Transportation PROCEDURES FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Pt. 40, App. G Appendix G to Part 40—Alcohol Testing Form The following form is the...

20 CFR 405.325 - Issues before an administrative law judge.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Issues before an administrative law judge. 405.325 Section 405.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION ADMINISTRATIVE REVIEW PROCESS FOR ADJUDICATING INITIAL DISABILITY CLAIMS Administrative Law Judge Hearing § 405.325 Issues...

9 CFR 93.325 - Horses from Mexico.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Horses from Mexico. 93.325 Section 93... CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.325 Horses from Mexico. Horses offered for entry from Mexico shall be inspected as provided in §§ 93.306 and 93.323; shall be accompanied by a...

9 CFR 93.325 - Horses from Mexico.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Horses from Mexico. 93.325 Section 93... CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.325 Horses from Mexico. Horses offered for entry from Mexico shall be inspected as provided in §§ 93.306 and 93.323; shall be accompanied by a...

9 CFR 93.325 - Horses from Mexico.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Horses from Mexico. 93.325 Section 93... CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.325 Horses from Mexico. Horses offered for entry from Mexico shall be inspected as provided in §§ 93.306 and 93.323; shall be accompanied by a...

9 CFR 93.325 - Horses from Mexico.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Horses from Mexico. 93.325 Section 93... CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.325 Horses from Mexico. Horses offered for entry from Mexico shall be inspected as provided in §§ 93.306 and 93.323; shall be accompanied by a...

9 CFR 93.325 - Horses from Mexico.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Horses from Mexico. 93.325 Section 93... CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.325 Horses from Mexico. Horses offered for entry from Mexico shall be inspected as provided in §§ 93.306 and 93.323; shall be accompanied by a...

24 CFR 1720.325 - Motions for dismissal.

Code of Federal Regulations, 2012 CFR

2012-04-01

... law judge shall file a decision in accordance with the provisions of § 1720.525. If such a motion is... 24 Housing and Urban Development 5 2012-04-01 2012-04-01 false Motions for dismissal. 1720.325... Adjudicatory Proceedings Motions § 1720.325 Motions for dismissal. (a) A motion to dismiss may be made at any...

24 CFR 1720.325 - Motions for dismissal.

Code of Federal Regulations, 2013 CFR

2013-04-01

... law judge shall file a decision in accordance with the provisions of § 1720.525. If such a motion is... 24 Housing and Urban Development 5 2013-04-01 2013-04-01 false Motions for dismissal. 1720.325... Adjudicatory Proceedings Motions § 1720.325 Motions for dismissal. (a) A motion to dismiss may be made at any...

24 CFR 1720.325 - Motions for dismissal.

Code of Federal Regulations, 2014 CFR

2014-04-01

... law judge shall file a decision in accordance with the provisions of § 1720.525. If such a motion is... 24 Housing and Urban Development 5 2014-04-01 2014-04-01 false Motions for dismissal. 1720.325... Adjudicatory Proceedings Motions § 1720.325 Motions for dismissal. (a) A motion to dismiss may be made at any...

24 CFR 1720.325 - Motions for dismissal.

Code of Federal Regulations, 2010 CFR

2010-04-01

... law judge shall file a decision in accordance with the provisions of § 1720.525. If such a motion is... 24 Housing and Urban Development 5 2010-04-01 2010-04-01 false Motions for dismissal. 1720.325... Adjudicatory Proceedings Motions § 1720.325 Motions for dismissal. (a) A motion to dismiss may be made at any...

24 CFR 1720.325 - Motions for dismissal.

Code of Federal Regulations, 2011 CFR

2011-04-01

... law judge shall file a decision in accordance with the provisions of § 1720.525. If such a motion is... 24 Housing and Urban Development 5 2011-04-01 2011-04-01 false Motions for dismissal. 1720.325... Adjudicatory Proceedings Motions § 1720.325 Motions for dismissal. (a) A motion to dismiss may be made at any...

12 CFR 563g.7 - Form, content, and accounting.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Form, content, and accounting. 563g.7 Section... § 563g.7 Form, content, and accounting. (a) Form and content. Any offering circular or amendment filed... which they are made, not misleading. (b) Accounting requirements. To be declared effective an offering...

20 CFR 416.325 - When an application is considered filed.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false When an application is considered filed. 416.325 Section 416.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Filing of Applications Applications § 416.325 When an application is...

20 CFR 416.325 - When an application is considered filed.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false When an application is considered filed. 416.325 Section 416.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Filing of Applications Applications § 416.325 When an application is...

20 CFR 416.325 - When an application is considered filed.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false When an application is considered filed. 416.325 Section 416.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Filing of Applications Applications § 416.325 When an application is...

20 CFR 416.325 - When an application is considered filed.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false When an application is considered filed. 416.325 Section 416.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Filing of Applications Applications § 416.325 When an application is...

20 CFR 408.325 - When is your application considered filed?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false When is your application considered filed? 408.325 Section 408.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SPECIAL BENEFITS FOR CERTAIN WORLD WAR II VETERANS Filing Applications Filing Your Application § 408.325 When is your...

20 CFR 408.325 - When is your application considered filed?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false When is your application considered filed? 408.325 Section 408.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SPECIAL BENEFITS FOR CERTAIN WORLD WAR II VETERANS Filing Applications Filing Your Application § 408.325 When is your...

20 CFR 416.325 - When an application is considered filed.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false When an application is considered filed. 416.325 Section 416.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Filing of Applications Applications § 416.325 When an application is...

14 CFR 171.325 - Maintenance and operations requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Maintenance and operations requirements. 171.325 Section 171.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... service. (7) Detailed and specific maintenance procedures and servicing guides stating the frequency of...

14 CFR 171.325 - Maintenance and operations requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Maintenance and operations requirements. 171.325 Section 171.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... service. (7) Detailed and specific maintenance procedures and servicing guides stating the frequency of...

14 CFR 171.325 - Maintenance and operations requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Maintenance and operations requirements. 171.325 Section 171.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... service. (7) Detailed and specific maintenance procedures and servicing guides stating the frequency of...

14 CFR 171.325 - Maintenance and operations requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Maintenance and operations requirements. 171.325 Section 171.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... service. (7) Detailed and specific maintenance procedures and servicing guides stating the frequency of...

14 CFR 171.325 - Maintenance and operations requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Maintenance and operations requirements. 171.325 Section 171.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... service. (7) Detailed and specific maintenance procedures and servicing guides stating the frequency of...

15 CFR 325.16 - Protecting confidentiality of information.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Protecting confidentiality of information. 325.16 Section 325.16 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS...

49 CFR 325.33 - Site characteristics; highway operations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Section 325.33 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.33 Site... or utility poles. (2) Rural mailboxes. (3) Traffic railings of any type of construction except solid...

49 CFR 325.33 - Site characteristics; highway operations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Section 325.33 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.33 Site... or utility poles. (2) Rural mailboxes. (3) Traffic railings of any type of construction except solid...

49 CFR 325.33 - Site characteristics; highway operations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Section 325.33 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.33 Site... or utility poles. (2) Rural mailboxes. (3) Traffic railings of any type of construction except solid...

49 CFR 325.33 - Site characteristics; highway operations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Section 325.33 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.33 Site... or utility poles. (2) Rural mailboxes. (3) Traffic railings of any type of construction except solid...

15 CFR 325.12 - Returning the applicant's documents.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Returning the applicant's documents. 325.12 Section 325.12 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE...

15 CFR 325.8 - Expediting the certification process.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Expediting the certification process. 325.8 Section 325.8 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE...

49 CFR 325.39 - Measurement procedure; highway operations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Measurement procedure; highway operations. 325.39... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.39 Measurement procedure; highway operations. (a) In accordance with the rules in this subpart, a measurement...

49 CFR 325.39 - Measurement procedure; highway operations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 5 2012-10-01 2012-10-01 false Measurement procedure; highway operations. 325.39... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.39 Measurement procedure; highway operations. (a) In accordance with the rules in this subpart, a measurement...

49 CFR 325.39 - Measurement procedure; highway operations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Measurement procedure; highway operations. 325.39... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.39 Measurement procedure; highway operations. (a) In accordance with the rules in this subpart, a measurement...

49 CFR 325.55 - Ambient conditions; stationary test.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Ambient conditions; stationary test. 325.55 Section 325.55 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL REGULATIONS COMPLIANCE WITH INTERSTATE MOTOR CARRIER NOISE EMISSION STANDARDS...

49 CFR 325.59 - Measurement procedure; stationary test.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Measurement procedure; stationary test. 325.59 Section 325.59 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL REGULATIONS COMPLIANCE WITH INTERSTATE MOTOR CARRIER NOISE EMISSION STANDARDS...

29 CFR 1952.325 - Level of Federal enforcement.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 9 2010-07-01 2010-07-01 false Level of Federal enforcement. 1952.325 Section 1952.325 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... Level of Federal enforcement. (a) As a result of the Assistant Secretary's determination granting final...

49 CFR 325.35 - Ambient conditions; highway operations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Ambient conditions; highway operations. 325.35... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.35 Ambient conditions; highway operations. (a)(1) Sound. The ambient A-weighted sound level at the microphone...

49 CFR 325.35 - Ambient conditions; highway operations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Ambient conditions; highway operations. 325.35... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Highway Operations § 325.35 Ambient conditions; highway operations. (a)(1) Sound. The ambient A-weighted sound level at the microphone...

50 CFR 600.325 - National Standard 4-Allocations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... promote conservation (in the sense of wise use) by optimizing the yield in terms of size, value, market... Section 600.325 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND....325 National Standard 4—Allocations. (a) Standard 4. Conservation and management measures shall not...

21 CFR 173.325 - Acidified sodium chlorite solutions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Acidified sodium chlorite solutions. 173.325... HUMAN CONSUMPTION Specific Usage Additives § 173.325 Acidified sodium chlorite solutions. Acidified sodium chlorite solutions may be safely used in accordance with the following prescribed conditions: (a...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

50 CFR 600.325 - National Standard 4-Allocations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... communities, efficiency of various types of gear used in the fishery, transferability of effort to and impact... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false National Standard 4-Allocations. 600.325 Section 600.325 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND...

50 CFR 600.325 - National Standard 4-Allocations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... communities, efficiency of various types of gear used in the fishery, transferability of effort to and impact... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false National Standard 4-Allocations. 600.325 Section 600.325 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND...

50 CFR 600.325 - National Standard 4-Allocations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... communities, efficiency of various types of gear used in the fishery, transferability of effort to and impact... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false National Standard 4-Allocations. 600.325 Section 600.325 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND...

21 CFR 173.325 - Acidified sodium chlorite solutions.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Acidified sodium chlorite solutions. 173.325 Section 173.325 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) SECONDARY DIRECT FOOD ADDITIVES PERMITTED IN FOOD FOR...

7 CFR 1207.325 - Procedure.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan National Potato Promotion Board § 1207.325 Procedure. (a) Each State...

7 CFR 1205.325 - Selection.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE COTTON RESEARCH AND PROMOTION Cotton Research and Promotion Order Cotton Board § 1205.325 Selection. From the nominations made pursuant to...

7 CFR 1205.325 - Selection.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE COTTON RESEARCH AND PROMOTION Cotton Research and Promotion Order Cotton Board § 1205.325 Selection. From the nominations made pursuant to...

7 CFR 1205.325 - Selection.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE COTTON RESEARCH AND PROMOTION Cotton Research and Promotion Order Cotton Board § 1205.325 Selection. From the nominations made pursuant to...

7 CFR 1205.325 - Selection.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE COTTON RESEARCH AND PROMOTION Cotton Research and Promotion Order Cotton Board § 1205.325 Selection. From the nominations made pursuant to...

7 CFR 1205.325 - Selection.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE COTTON RESEARCH AND PROMOTION Cotton Research and Promotion Order Cotton Board § 1205.325 Selection. From the nominations made pursuant to...

7 CFR 1207.325 - Procedure.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan National Potato Promotion Board § 1207.325 Procedure. (a) Each State...

7 CFR 1207.325 - Procedure.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan National Potato Promotion Board § 1207.325 Procedure. (a) Each State...

7 CFR 1207.325 - Procedure.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan National Potato Promotion Board § 1207.325 Procedure. (a) Each State...

7 CFR 1207.325 - Procedure.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan National Potato Promotion Board § 1207.325 Procedure. (a) Each State...

14 CFR 325.4 - State and local participation.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false State and local participation. 325.4... PROCEEDINGS) PROCEDURAL REGULATIONS ESSENTIAL AIR SERVICE PROCEDURES § 325.4 State and local participation. (a... addressed to: (1) The chief executive of the principal city, or other unit of local government at the...

47 CFR 80.325 - Control of distress traffic.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Control of distress traffic. 80.325 Section 80... Safety Procedures § 80.325 Control of distress traffic. (a) Distress traffic consists of all messages relating to the immediate assistance required by the mobile station in distress. In distress traffic, the...

21 CFR 173.325 - Acidified sodium chlorite solutions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Acidified sodium chlorite solutions. 173.325... § 173.325 Acidified sodium chlorite solutions. Acidified sodium chlorite solutions may be safely used in... solution of sodium chlorite (CAS Reg. No. 7758-19-2) with any generally recognized as safe (GRAS) acid. (b...

76 FR 11806 - Agency Information Collection Activities: Form G-1145, Extension of a Currently Approved...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-03

... notified via e-mail and/or text message on their cell phone that their application or petition has been accepted, they are requested to provide their e-mail address and/or cell phone number on Form G-1145, and...

Pneumo MSG-325 Lathe

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rhorer, R.L.

1980-01-01

The LASL Shop Department has recently installed a Pneumo MSG-325 two-axis contouring lathe. This one-microinch resolution lathe, which utilizes both air-bearing spindle and air bearing slide ways, was purchased primarily for miniature work, but can also single point diamond turn metal optics.

12 CFR 325.204 - Annual stress tests required.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 5 2013-01-01 2013-01-01 false Annual stress tests required. 325.204 Section... POLICY CAPITAL MAINTENANCE Annual Stress Test § 325.204 Annual stress tests required. (a) General... conduct a stress test on or before March 31 of each calendar year based on financial data as of September...

12 CFR 325.204 - Annual stress tests required.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 5 2014-01-01 2014-01-01 false Annual stress tests required. 325.204 Section... POLICY CAPITAL MAINTENANCE Annual Stress Test § 325.204 Annual stress tests required. (a) General... conduct a stress test on or before March 31 of each calendar year based on financial data as of September...

15 CFR 325.10 - Modifying or revoking a certificate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Modifying or revoking a certificate. 325.10 Section 325.10 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS REGULATIONS EXPORT TRADE...

45 CFR 149.325 - Requirements for eligibility of claims.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Section 149.325 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH CARE ACCESS REQUIREMENTS FOR THE EARLY RETIREE REINSURANCE PROGRAM Reimbursement Methods § 149.325 Requirements for eligibility of claims. A claim may be submitted only if it represents costs for health...

45 CFR 149.325 - Requirements for eligibility of claims.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Section 149.325 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH CARE ACCESS REQUIREMENTS FOR THE EARLY RETIREE REINSURANCE PROGRAM Reimbursement Methods § 149.325 Requirements for eligibility of claims. A claim may be submitted only if it represents costs for health...

36 CFR 13.325 - Most directly affected Native Corporation.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Corporation. 13.325 Section 13.325 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF... affected Native Corporation. (a) Before the award of the first visitor service authorization in a park area... Native Corporation interested in providing visitor services within the applicable park area to submit an...

36 CFR 13.325 - Most directly affected Native Corporation.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Corporation. 13.325 Section 13.325 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF... affected Native Corporation. (a) Before the award of the first visitor service authorization in a park area... Native Corporation interested in providing visitor services within the applicable park area to submit an...

36 CFR 13.325 - Most directly affected Native Corporation.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Corporation. 13.325 Section 13.325 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF... affected Native Corporation. (a) Before the award of the first visitor service authorization in a park area... Native Corporation interested in providing visitor services within the applicable park area to submit an...

36 CFR 13.325 - Most directly affected Native Corporation.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Corporation. 13.325 Section 13.325 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF... affected Native Corporation. (a) Before the award of the first visitor service authorization in a park area... Native Corporation interested in providing visitor services within the applicable park area to submit an...

36 CFR 13.325 - Most directly affected Native Corporation.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Corporation. 13.325 Section 13.325 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF... affected Native Corporation. (a) Before the award of the first visitor service authorization in a park area... Native Corporation interested in providing visitor services within the applicable park area to submit an...

46 CFR 154.325 - Accommodation, service, and control spaces.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Accommodation, service, and control spaces. 154.325... Equipment Ship Arrangements § 154.325 Accommodation, service, and control spaces. (a) Accommodation, service, and control spaces must be outside the cargo area. (b) If a hold space having a cargo containment...

46 CFR 154.325 - Accommodation, service, and control spaces.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Accommodation, service, and control spaces. 154.325... Equipment Ship Arrangements § 154.325 Accommodation, service, and control spaces. (a) Accommodation, service, and control spaces must be outside the cargo area. (b) If a hold space having a cargo containment...

46 CFR 154.325 - Accommodation, service, and control spaces.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Accommodation, service, and control spaces. 154.325... Equipment Ship Arrangements § 154.325 Accommodation, service, and control spaces. (a) Accommodation, service, and control spaces must be outside the cargo area. (b) If a hold space having a cargo containment...

40 CFR 158.325 - Description of materials used to produce the product.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Description of materials used to produce the product. 158.325 Section 158.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.325 Description of...

40 CFR 158.325 - Description of materials used to produce the product.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Description of materials used to produce the product. 158.325 Section 158.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.325 Description of...

40 CFR 158.325 - Description of materials used to produce the product.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Description of materials used to produce the product. 158.325 Section 158.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.325 Description of...

40 CFR 158.325 - Description of materials used to produce the product.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Description of materials used to produce the product. 158.325 Section 158.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.325 Description of...

40 CFR 158.325 - Description of materials used to produce the product.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Description of materials used to produce the product. 158.325 Section 158.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.325 Description of...

77 FR 3278 - Agency Information Collection Activities: Form G-1145; Revision of a Currently Approved...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-23

... households. If an applicant or petitioner wants to be notified via email and/or text message on their cell phone that their application or petition has been accepted, they are requested to provide their email address and/or cell phone number on the E-Notification of Application/Petition Acceptance, Form G-1145...

L-325 Sagebrush Habitat Mitigation Project: FY2008 Compensation Area Monitoring Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Durham, Robin E.; Sackschewsky, Michael R.

2008-09-30

This document provides a review and status of activities conducted in support of the Fluor Daniel Hanford Company (Fluor) Mitigation Action Plan (MAP) for Project L-325, Electrical Utility Upgrades. It includes time-zero monitoring results for planting activities conducted in January 2008, annual survival monitoring for all planting years (2007 and 2008), and recommendations for the successful completion of DOE habitat mitigation commitments for this project.

76 FR 35509 - Proposed Collection; Comment Request for Form 1099-G

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-17

... 1099-G AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for comments... Form 1099-G, Certain Government Payments. DATES: Written comments should be received on or before... . SUPPLEMENTARY INFORMATION: Title: Certain Government Payments. OMB Number: 1545-0120. Form Number: 1099-G...

21 CFR 522.1696 - Penicillin G procaine injectable dosage forms.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 6 2014-04-01 2014-04-01 false Penicillin G procaine injectable dosage forms. 522.1696 Section 522.1696 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... ANIMAL DRUGS § 522.1696 Penicillin G procaine injectable dosage forms. ...

Effectiveness of TC-325 (Hemospray) for treatment of diffuse or refractory upper gastrointestinal bleeding - a single center experience.

PubMed

Cahyadi, Oscar; Bauder, Markus; Meier, Benjamin; Caca, Karel; Schmidt, Arthur

2017-11-01

 TC-325 (Hemospray, Cook Medical) is a powder agent for endoscopic hemostasis in patients with upper gastrointestinal bleeding (UGIB). Although most publications are based on case-reports and retrospective studies, data on efficacy are promising. Here we report our experience with TC-325 for diffuse or refractory UGIB.  Data on patients receiving TC-325 for endoscopic hemostasis from November 2013 to February 2017 at our center were analyzed retrospectively. Primary endpoints were technical success (successful immediate hemostasis) and clinical success (effective hemostasis and no recurrent bleeding). Secondary endpoints were recurrent bleeding within 3 and 7 days, hospital mortality and TC-325 associated complications. TC-325 was used for bleeding not amenable to standard endoscopic treatment (e. g. diffuse bleeding) or as salvage therapy after failure of conventional methods.  Fifty-two patients received TC-325 treatment. Most of the patients were treated for peptic ulcer bleeding (18/52 patients, 34.6 %) and post-interventional bleeding (13/52 patients, 25 %). Hemospray was used in 23/52 (44.2 %) patients as monotherapy and in 29/52 (55.8 %) patients as a salvage therapy. Application of the powder on the bleeding source was successful in all patients with no therapy-related adverse events (AEs). Immediate hemostasis was achieved in 51/52 (98.1 %) patients. Recurrent bleeding within 3 and 7 days was observed in 22/51 and 25/51 patients respectively (43.1 % and 49 %). The overall clinical success was 56.9 % on day 3 and 51 % on day 7. Total mortality was 15.4 % (8 patients), bleeding associated mortality was 3.8 % (2 patients). There were no therapy-related AEs.  TC-325 showed a high technical success rate as monotherapy for bleeding sources not amenable to standard methods or as an "add-on" therapy after unsuccessful hemostasis. However, rebleeding was frequent in this cohort and further studies are warranted to exactly define a

12 CFR 325.207 - Publication of stress test results.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 5 2013-01-01 2013-01-01 false Publication of stress test results. 325.207... GENERAL POLICY CAPITAL MAINTENANCE Annual Stress Test § 325.207 Publication of stress test results. (a) Publication date. (1) A $10 billion to $50 billion covered bank must publish a summary of the results of its...

12 CFR 325.207 - Publication of stress test results.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 5 2014-01-01 2014-01-01 false Publication of stress test results. 325.207... GENERAL POLICY CAPITAL MAINTENANCE Annual Stress Test § 325.207 Publication of stress test results. (a) Publication date. (1) A $10 billion to $50 billion covered bank must publish a summary of the results of its...

40 CFR 63.325 - Determination of equivalent emission control technology.

Code of Federal Regulations, 2010 CFR

2010-07-01

... control technology. 63.325 Section 63.325 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Determination of equivalent emission control technology. (a) Any person requesting that the use of certain... equivalent emission reductions: (1) Diagrams, as appropriate, illustrating the emission control technology...

DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity.

PubMed

Usui, Kenji; Okada, Arisa; Sakashita, Shungo; Shimooka, Masayuki; Tsuruoka, Takaaki; Nakano, Shu-Ichi; Miyoshi, Daisuke; Mashima, Tsukasa; Katahira, Masato; Hamada, Yoshio

2017-11-16

The development of a switching system for guanine nanowire (G-wire) formation by external signals is important for nanobiotechnological applications. Here, we demonstrate a DNA nanostructural switch (G-wire <--> particles) using a designed peptide and a protease. The peptide consists of a PNA sequence for inducing DNA to form DNA-PNA hybrid G-quadruplex structures, and a protease substrate sequence acting as a switching module that is dependent on the activity of a particular protease. Micro-scale analyses via TEM and AFM showed that G-rich DNA alone forms G-wires in the presence of Ca 2+ , and that the peptide disrupted this formation, resulting in the formation of particles. The addition of the protease and digestion of the peptide regenerated the G-wires. Macro-scale analyses by DLS, zeta potential, CD, and gel filtration were in agreement with the microscopic observations. These results imply that the secondary structure change (DNA G-quadruplex <--> DNA/PNA hybrid structure) induces a change in the well-formed nanostructure (G-wire <--> particles). Our findings demonstrate a control system for forming DNA G-wire structures dependent on protease activity using designed peptides. Such systems hold promise for regulating the formation of nanowire for various applications, including electronic circuits for use in nanobiotechnologies.

49 CFR 325.73 - Microphone distance correction factors. 1

Code of Federal Regulations, 2011 CFR

2011-10-01

... factors. 1 1 Table 1, in § 325.7 is a tabulation of the maximum allowable sound level readings taking into... target point is other than 50 feet (15.2 m), the maximum observed sound level reading generated by the... observed sound level readings generated by the motor vehicle in accordance with § 325.59 of this part shall...

49 CFR 325.73 - Microphone distance correction factors. 1

Code of Federal Regulations, 2010 CFR

2010-10-01

... factors. 1 1 Table 1, in § 325.7 is a tabulation of the maximum allowable sound level readings taking into... target point is other than 50 feet (15.2 m), the maximum observed sound level reading generated by the... observed sound level readings generated by the motor vehicle in accordance with § 325.59 of this part shall...

49 CFR 325.5 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF TRANSPORTATION GENERAL REGULATIONS COMPLIANCE WITH INTERSTATE MOTOR CARRIER NOISE EMISSION STANDARDS General Provisions § 325.5 Definitions. (a) Statutory definitions. All terms defined in the Noise...) Definitions in standards. All terms defined in § 202.10 of the Interstate Motor Carrier Noise Emission...

49 CFR 325.5 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., DEPARTMENT OF TRANSPORTATION GENERAL REGULATIONS COMPLIANCE WITH INTERSTATE MOTOR CARRIER NOISE EMISSION STANDARDS General Provisions § 325.5 Definitions. (a) Statutory definitions. All terms defined in the Noise...) Definitions in standards. All terms defined in § 202.10 of the Interstate Motor Carrier Noise Emission...

20 CFR 325.1 - General.

Code of Federal Regulations, 2010 CFR

2010-04-01

...' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.1 General. (a) Day of unemployment. A “day of unemployment.... The amount of compensable days of unemployment shall be computed in accordance with this section. (b...

20 CFR 325.1 - General.

Code of Federal Regulations, 2011 CFR

2011-04-01

...' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.1 General. (a) Day of unemployment. A “day of unemployment.... The amount of compensable days of unemployment shall be computed in accordance with this section. (b...

20 CFR 325.1 - General.

Code of Federal Regulations, 2013 CFR

2013-04-01

...' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.1 General. (a) Day of unemployment. A “day of unemployment.... The amount of compensable days of unemployment shall be computed in accordance with this section. (b...

20 CFR 325.1 - General.

Code of Federal Regulations, 2012 CFR

2012-04-01

...' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.1 General. (a) Day of unemployment. A “day of unemployment.... The amount of compensable days of unemployment shall be computed in accordance with this section. (b...

20 CFR 325.1 - General.

Code of Federal Regulations, 2014 CFR

2014-04-01

...' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.1 General. (a) Day of unemployment. A “day of unemployment.... The amount of compensable days of unemployment shall be computed in accordance with this section. (b...

9 CFR 3.25 - Facilities, general.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Pigs and Hamsters Facilities and Operating Standards § 3.25 Facilities, general. (a) Structural strength. Indoor and outdoor housing facilities for guinea pigs or hamsters shall be structurally sound and...

9 CFR 3.25 - Facilities, general.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Pigs and Hamsters Facilities and Operating Standards § 3.25 Facilities, general. (a) Structural strength. Indoor and outdoor housing facilities for guinea pigs or hamsters shall be structurally sound and...

9 CFR 3.25 - Facilities, general.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Pigs and Hamsters Facilities and Operating Standards § 3.25 Facilities, general. (a) Structural strength. Indoor and outdoor housing facilities for guinea pigs or hamsters shall be structurally sound and...

9 CFR 3.25 - Facilities, general.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Pigs and Hamsters Facilities and Operating Standards § 3.25 Facilities, general. (a) Structural strength. Indoor and outdoor housing facilities for guinea pigs or hamsters shall be structurally sound and...

Identification of G-quadruplex forming sequences in three manatee papillomaviruses

PubMed Central

Zahin, Maryam; Dean, William L.; Ghim, Shin-je; Joh, Joongho; Gray, Robert D.; Khanal, Sujita; Bossart, Gregory D.; Mignucci-Giannoni, Antonio A.; Rouchka, Eric C.; Jenson, Alfred B.; Trent, John O.; Chaires, Jonathan B.

2018-01-01

The Florida manatee (Trichechus manatus latirotris) is a threatened aquatic mammal in United States coastal waters. Over the past decade, the appearance of papillomavirus-induced lesions and viral papillomatosis in manatees has been a concern for those involved in the management and rehabilitation of this species. To date, three manatee papillomaviruses (TmPVs) have been identified in Florida manatees, one forming cutaneous lesions (TmPV1) and two forming genital lesions (TmPV3 and TmPV4). We identified DNA sequences with the potential to form G-quadruplex structures (G4) across the three genomes. G4 were located on both DNA strands and across coding and non-coding regions on all TmPVs, offering multiple targets for viral control. Although G4 have been identified in several viral genomes, including human PVs, most research has focused on canonical structures comprised of three G-tetrads. In contrast, the vast majority of sequences we identified would allow the formation of non-canonical structures with only two G-tetrads. Our biophysical analysis confirmed the formation of G4 with parallel topology in three such sequences from the E2 region. Two of the structures appear comprised of multiple stacked two G-tetrad structures, perhaps serving to increase structural stability. Computational analysis demonstrated enrichment of G4 sequences on all TmPVs on the reverse strand in the E2/E4 region and on both strands in the L2 region. Several G4 sequences occurred at similar regional locations on all PVs, most notably on the reverse strand in the E2 region. In other cases, G4 were identified at similar regional locations only on PVs forming genital lesions. On all TmPVs, G4 sequences were located in the non-coding region near putative E2 binding sites. Together, these findings suggest that G4 are possible regulatory elements in TmPVs. PMID:29630682

12 CFR 325.5 - Miscellaneous.

Code of Federal Regulations, 2013 CFR

2013-01-01

... MAINTENANCE Minimum Capital Requirements § 325.5 Miscellaneous. (a) Intangible assets. Any intangible assets...-enhancing interest-only strips on a basis that is net of a proportional amount of any associated deferred tax liability recorded on the balance sheet. Any deferred tax liability netted in this manner cannot...

12 CFR 325.5 - Miscellaneous.

Code of Federal Regulations, 2014 CFR

2014-01-01

... MAINTENANCE Minimum Capital Requirements § 325.5 Miscellaneous. (a) Intangible assets. Any intangible assets...-enhancing interest-only strips on a basis that is net of a proportional amount of any associated deferred tax liability recorded on the balance sheet. Any deferred tax liability netted in this manner cannot...

32 CFR 700.325 - The Assistant Secretary of the Navy (Installations and Environment).

Code of Federal Regulations, 2010 CFR

2010-07-01

... (Installations and Environment). 700.325 Section 700.325 National Defense Department of Defense (Continued... Assistants § 700.325 The Assistant Secretary of the Navy (Installations and Environment). The Assistant Secretary of the Navy (Installations and Environment) is responsible for: (a) Policy relating to Navy...

Synthesis of potent G-quadruplex binders of macrocyclic heptaoxazole and evaluation of their activities.

PubMed

Tera, Masayuki; Iida, Keisuke; Shin-ya, Kazuo; Nagasawa, Kazuo

2009-01-01

Guanine-rich DNA sequences form unique three-dimensional conformation known as G-quadruplexes (G-q). G-q structures have been found in telomere and in some oncogene promoter. Recently, it was suggested that G-q showed some biological activities including telomere shortening and transcriptional regulation. In this paper, we synthesized selective G-q binders and evaluated of their biological activities.

12 CFR 325.2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... which the FDIC is the appropriate federal banking agency pursuant to section 3(q) of the FDI Act (12 U.S.C. 1813(q)). (d) Common stockholders' equity means the sum of common stock and related surplus... conditions, limitations, and restrictions described in § 325.5 (f). (q) Noncumulative perpetual preferred...

12 CFR 325.2 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... which the FDIC is the appropriate federal banking agency pursuant to section 3(q) of the FDI Act (12 U.S.C. 1813(q)). (d) Common stockholders' equity means the sum of common stock and related surplus... conditions, limitations, and restrictions described in § 325.5 (f). (q) Noncumulative perpetual preferred...

12 CFR 325.2 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... which the FDIC is the appropriate federal banking agency pursuant to section 3(q) of the FDI Act (12 U.S.C. 1813(q)). (d) Common stockholders' equity means the sum of common stock and related surplus... conditions, limitations, and restrictions described in § 325.5 (f). (q) Noncumulative perpetual preferred...

12 CFR 325.2 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... which the FDIC is the appropriate federal banking agency pursuant to section 3(q) of the FDI Act (12 U.S.C. 1813(q)). (d) Common stockholders' equity means the sum of common stock and related surplus... conditions, limitations, and restrictions described in § 325.5 (f). (q) Noncumulative perpetual preferred...

12 CFR 325.202 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... MAINTENANCE Annual Stress Test § 325.202 Definitions. For purposes of this subpart— (a) Adverse scenario means... appropriate for use in the company-run stress tests, including, but not limited to, baseline, adverse, and... of the Federal Deposit Insurance Act (12 U.S.C. 1813). (l) Stress test means the process to assess...

12 CFR 325.203 - Applicability.

Code of Federal Regulations, 2014 CFR

2014-01-01

... MAINTENANCE Annual Stress Test § 325.203 Applicability. (a) First stress test for covered banks subject to stress testing requirements as of October 15, 2012. (1) A $10 billion to $50 billion covered bank as of October 15, 2012 must conduct its first stress test under this subpart using financial statement data as...

12 CFR 325.203 - Applicability.

Code of Federal Regulations, 2013 CFR

2013-01-01

... MAINTENANCE Annual Stress Test § 325.203 Applicability. (a) First stress test for covered banks subject to stress testing requirements as of October 15, 2012. (1) A $10 billion to $50 billion covered bank as of October 15, 2012 must conduct its first stress test under this subpart using financial statement data as...

12 CFR 325.202 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... MAINTENANCE Annual Stress Test § 325.202 Definitions. For purposes of this subpart— (a) Adverse scenario means... appropriate for use in the company-run stress tests, including, but not limited to, baseline, adverse, and... of the Federal Deposit Insurance Act (12 U.S.C. 1813). (l) Stress test means the process to assess...

9 CFR 325.2 - Parcel post and ferries deemed carriers.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Parcel post and ferries deemed carriers. 325.2 Section 325.2 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF... to transportation by ferry of any products loaded on a truck or other vehicle, or otherwise moved by...

15 CFR 325.3 - Applying for a certificate of review.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Applying for a certificate of review. 325.3 Section 325.3 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade... which it deals, and the places where it does business. This description may be supplemented by a chart...

The α Subunit of the G Protein G13 Regulates Activity of One or More Gli Transcription Factors Independently of Smoothened*

PubMed Central

Douglas, Andrew E.; Heim, Jennifer A.; Shen, Feng; Almada, Luciana L.; Riobo, Natalia A.; Fernández-Zapico, Martin E.; Manning, David R.

2011-01-01

Smoothened (Smo) is a seven-transmembrane (7-TM) receptor that is essential to most actions of the Hedgehog family of morphogens. We found previously that Smo couples to members of the Gi family of heterotrimeric G proteins, which in some cases are integral although alone insufficient in the activation of Gli transcription factors through Hedgehog signaling. In response to a report that the G12/13 family is relevant to Hedgehog signaling as well, we re-evaluated the coupling of Smo to one member of this family, G13, and investigated the capacity of this and other G proteins to activate one or more of forms of Gli. We found no evidence that Smo couples directly to G13. We found nonetheless that Gα13 and to some extent Gαq and Gα12 are able to effect activation of Gli(s). This capacity is realized in some cells, e.g. C3H10T1/2, MC3T3, and pancreatic cancer cells, but not all cells. The mechanism employed is distinct from that achieved through canonical Hedgehog signaling, as the activation does not involve autocrine signaling or in any other way require active Smo and does not necessarily involve enhanced transcription of Gli1. The activation by Gα13 can be replicated through a Gq/G12/13-coupled receptor, CCKA, and is attenuated by inhibitors of p38 mitogen-activated protein kinase and Tec tyrosine kinases. We posit that G proteins, and perhaps G13 in particular, provide access to Gli that is independent of Smo and that they thus establish a basis for control of at least some forms of Gli-mediated transcription apart from Hedgehogs. PMID:21757753

Frequency and severity of reactions to a 325-mg aspirin dose during desensitization.

PubMed

Schuler, Charles F; Baldwin, James L; Baptist, Alan P

2017-03-01

The frequency with which patients with aspirin-exacerbated respiratory disease (AERD) react to 325 mg of aspirin during aspirin desensitization, or fail to react at all, is not fully known. To determine the rate and type of reaction at 325 mg of aspirin during desensitization. A retrospective study of 104 patients who underwent aspirin desensitization from 2010 to 2016 was performed. A standard desensitization protocol (starting at 20-40 mg, progressing through 325 mg, and extinguishing reactions by dose repetition) was used. Reactions were defined by upper respiratory tract symptoms, lower respiratory tract symptoms, and/or forced expiratory volume in 1 second decrease of 15% or greater. Patients who did and did not react were compared by logistic regression. Eighty-four patients reacted (81%) and 20 did not (19%). Seventy-seven patients who had a provoking reaction at 162 mg of aspirin or less subsequently extinguished their reactions before they reached a dose of 325 mg and had no problems at that dose; one subsequent 325-mg reaction occurred during a protocol violation. One initial provoking reaction to 325 mg occurred. Both 325-mg reactions were mild, and neither met the forced expiratory volume in 1 second criterion for a clinically meaningful change. The remaining 5 patients could not complete the protocol because of persistent reactions or social reasons. Reactors were more likely to have had asthma for more than 10 years than nonreactors (odds ratio, 3.2; 95% confidence interval, 1.0-10.3; P = .05). During aspirin desensitization for AERD, provoking reactions at the 325-mg dose are rare (1%) and mild. Patients who react at 162 mg or less and extinguish their reactions may be able to administer the 325-mg dose at home. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

Talin-dependent integrin activation is required for fibrin clot retraction by platelets

PubMed Central

Haling, Jacob R.; Monkley, Susan J.; Critchley, David R.

2011-01-01

Talin functions both as a regulator of integrin affinity and as an important mechanical link between integrins and the cytoskeleton. Using genetic deletion of talin, we show for the first time that the capacity of talin to activate integrins is required for fibrin clot retraction by platelets. To further dissect which talin functions are required for this process, we tested clot retraction in platelets expressing a talin1(L325R) mutant that binds to integrins, but exhibits impaired integrin activation ascribable to disruption of the interaction between talin and the membrane-proximal region (MPR) in the β-integrin cytoplasmic domain. Talin-deficient and talin1(L325R) platelets were defective in retracting fibrin clots. However, the defect in clot retraction in talin1(L325R) platelets, but not talin-deficient platelets, was rescued by extrinsically activating integrins with manganese, thereby proving that integrin activation is required and showing that talin1(L325R) can form functional links to the actin cytoskeleton. PMID:20971947

Up-regulation of miR-325-3p suppresses pineal aralkylamine N-acetyltransferase (Aanat) after neonatal hypoxia-ischemia brain injury in rats.

PubMed

Yang, Yuanyuan; Sun, Bin; Huang, Jian; Xu, Lixiao; Pan, Jian; Fang, Chen; Li, Mei; Li, Gen; Tao, Yanfang; Yang, Xiaofeng; Wu, Ying; Miao, Po; Wang, Ying; Li, Hong; Ren, Jing; Zhan, Meiqin; Fang, Yiping; Feng, Xing; Ding, Xin

2017-08-01

Survivors of hypoxic-ischemic brain damage (HIBD), besides impairment of psychomotor development, often develop circadian rhythm disorders, although the underlying mechanisms are largely unknown. Here, we first verified that mRNA and protein expression of pineal aralkylamine N-acetyltransferase (Aanat), a key regulator for melatonin (MT) synthesis, along with MT, were severely impaired after HIBD. In addition, we demonstrated that neonatal HIBD disrupted the circadian rhythmicity of locomotor activities in juvenile rats. Based on bioinformatics analysis of a high throughput screening of miRNA expression changes after HIBD (Ding et al., 2015), we identified one microRNA, miR-325-3p, as a potential candidate responsible for the down regulation of Aanat after HIBD. Luciferase reporter assays demonstrated a specific interaction between miR-325-3p and Aanat mRNA 3'-UTR. miR-325-3p blocked norepinephrine (NE) induced Aanat activation in cultured pinealocytes. In addition, miR-325-3p inhibition partially rescued Aanat induction by NE, which was significantly reduced under oxygen glucose deprivation. By elucidating the role of pineal miR-325-3p on Aanat expression upon injury, our study provides new insights into the pathophysiological mechanisms of circadian dysfunction and potential therapeutic targets after HIBD. Copyright © 2017 Elsevier B.V. All rights reserved.

20 CFR 325.6 - Verification procedures.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.6 Verification procedures. The Board's procedures for adjudicating and processing applications and claims for unemployment benefits filed pursuant to this part will...

20 CFR 325.6 - Verification procedures.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.6 Verification procedures. The Board's procedures for adjudicating and processing applications and claims for unemployment benefits filed pursuant to this part will...

20 CFR 325.6 - Verification procedures.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.6 Verification procedures. The Board's procedures for adjudicating and processing applications and claims for unemployment benefits filed pursuant to this part will...

20 CFR 325.6 - Verification procedures.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.6 Verification procedures. The Board's procedures for adjudicating and processing applications and claims for unemployment benefits filed pursuant to this part will...

20 CFR 325.6 - Verification procedures.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.6 Verification procedures. The Board's procedures for adjudicating and processing applications and claims for unemployment benefits filed pursuant to this part will...

Chemical constituents of Helichrysum italicum (Roth) G. Don essential oil and their antimicrobial activity against Gram-positive and Gram-negative bacteria, filamentous fungi and Candida albicans.

PubMed

Djihane, Bouzid; Wafa, Nouioua; Elkhamssa, Soltani; Pedro, De Haro Juan; Maria, Angeles Esteban; Mohamed Mihoub, Zerroug

2017-07-01

The aerial parts of Helichrysum italicum (Roth) G. Don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. The essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). The most oxygenated sesquiterpene compounds were α-Cedrene (13.61%), α-Curcumene (11.41%), Geranyl acetate (10.05%), Limonene (6.07%), Nerol (5.04%), Neryl acetate (4.91%) and α-Pinene (3.78%). The antimicrobial activity of the essential oil was assayed by using the disk diffusion method on Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Micrococcus luteus ATCC 4698, Klebsiella pneumonia ATCC 4352, Enterococcus cereus ATCC 2035, Bacillus cereus ATCC 10876, Staphylococcus epidermidis ATCC 12228, Bacillus subtilis ATCC 9372, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 49452, Proteus mirabilis ATCC 35659, Listeria monocytogenes ATCC 15313 and yeasts Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763 and fungi, Fusarium solani var. coeruleum , Aspergillus niger , Alternaria alternata , Ascochyta rabiei . H. italicum inhibited the growth of all the tested microorganisms except three bacteria, E. coli ATCC 25922, K. pneumonia ATCC 4352 and L. monocytogenes ATCC 15313. The most sensitive bacterium was E. cereus ATCC 2035 with minimum inhibitory and bactericidal concentrations of 0.79 μg ml -1 . A minimum fungistatic and fungicide concentration of 6.325 μg ml -1 and 12.65 μg ml -1 respectively was obtained with C. albicans ATCC 10231 and S. cerevisiae ATCC 9763. However the four fungi were more resistant with fungistatic minimum concentration ranging from 6.325 μg ml -1 to 50.6 μg ml -1 and a fungicide minimum concentration of 50

15 CFR 325.9 - Reconsidering an application that has been denied.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Reconsidering an application that has been denied. 325.9 Section 325.9 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS...

9 CFR 3.25 - Facilities, general.

Code of Federal Regulations, 2010 CFR

2010-01-01

... WELFARE STANDARDS Specifications for the Humane Handling, Care, Treatment, and Transportation of Guinea Pigs and Hamsters Facilities and Operating Standards § 3.25 Facilities, general. (a) Structural strength. Indoor and outdoor housing facilities for guinea pigs or hamsters shall be structurally sound and...

76 FR 36618 - Proposed Collection; Comment Request for Form W-2G

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-22

... Form W-2G, Certain Gambling Winnings. DATES: Written comments should be received on or before August 22... INFORMATION: Title: Certain Gambling Winnings. OMB Number: 1545-0238. Form Number: Form W-2G. Abstract: Internal Revenue Code sections 6041, 3402(q), and 3406 require payers of certain gambling winnings to...

21 CFR 522.1696 - Penicillin G procaine implantation and injectable dosage forms.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Penicillin G procaine implantation and injectable dosage forms. 522.1696 Section 522.1696 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... DOSAGE FORM NEW ANIMAL DRUGS § 522.1696 Penicillin G procaine implantation and injectable dosage forms. ...

21 CFR 522.1696 - Penicillin G procaine implantation and injectable dosage forms.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 6 2011-04-01 2011-04-01 false Penicillin G procaine implantation and injectable dosage forms. 522.1696 Section 522.1696 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... DOSAGE FORM NEW ANIMAL DRUGS § 522.1696 Penicillin G procaine implantation and injectable dosage forms. ...

21 CFR 522.1696 - Penicillin G procaine implantation and injectable dosage forms.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 6 2012-04-01 2012-04-01 false Penicillin G procaine implantation and injectable dosage forms. 522.1696 Section 522.1696 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... DOSAGE FORM NEW ANIMAL DRUGS § 522.1696 Penicillin G procaine implantation and injectable dosage forms. ...

21 CFR 522.1696 - Penicillin G procaine implantation and injectable dosage forms.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 6 2013-04-01 2013-04-01 false Penicillin G procaine implantation and injectable dosage forms. 522.1696 Section 522.1696 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... DOSAGE FORM NEW ANIMAL DRUGS § 522.1696 Penicillin G procaine implantation and injectable dosage forms. ...

Effectiveness of TC-325 (Hemospray) for treatment of diffuse or refractory upper gastrointestinal bleeding – a single center experience

PubMed Central

Cahyadi, Oscar; Bauder, Markus; Meier, Benjamin; Caca, Karel; Schmidt, Arthur

2017-01-01

Background and study aims  TC-325 (Hemospray, Cook Medical) is a powder agent for endoscopic hemostasis in patients with upper gastrointestinal bleeding (UGIB). Although most publications are based on case-reports and retrospective studies, data on efficacy are promising. Here we report our experience with TC-325 for diffuse or refractory UGIB. Patients and methods  Data on patients receiving TC-325 for endoscopic hemostasis from November 2013 to February 2017 at our center were analyzed retrospectively. Primary endpoints were technical success (successful immediate hemostasis) and clinical success (effective hemostasis and no recurrent bleeding). Secondary endpoints were recurrent bleeding within 3 and 7 days, hospital mortality and TC-325 associated complications. TC-325 was used for bleeding not amenable to standard endoscopic treatment (e. g. diffuse bleeding) or as salvage therapy after failure of conventional methods Results  Fifty-two patients received TC-325 treatment. Most of the patients were treated for peptic ulcer bleeding (18/52 patients, 34.6 %) and post-interventional bleeding (13/52 patients, 25 %). Hemospray was used in 23/52 (44.2 %) patients as monotherapy and in 29/52 (55.8 %) patients as a salvage therapy. Application of the powder on the bleeding source was successful in all patients with no therapy-related adverse events (AEs). Immediate hemostasis was achieved in 51/52 (98.1 %) patients. Recurrent bleeding within 3 and 7 days was observed in 22/51 and 25/51 patients respectively (43.1 % and 49 %). The overall clinical success was 56.9 % on day 3 and 51 % on day 7. Total mortality was 15.4 % (8 patients), bleeding associated mortality was 3.8 % (2 patients). There were no therapy-related AEs. Conclusions  TC-325 showed a high technical success rate as monotherapy for bleeding sources not amenable to standard methods or as an “add-on” therapy after unsuccessful hemostasis. However, rebleeding was frequent

49 CFR 325.37 - Location and operation of sound level measurement system; highway operations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... system; highway operations. 325.37 Section 325.37 Transportation Other Regulations Relating to...; Highway Operations § 325.37 Location and operation of sound level measurement system; highway operations..., the holder must orient himself/herself relative to the highway in a manner consistent with the...

49 CFR 325.37 - Location and operation of sound level measurement system; highway operations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... system; highway operations. 325.37 Section 325.37 Transportation Other Regulations Relating to...; Highway Operations § 325.37 Location and operation of sound level measurement system; highway operations..., the holder must orient himself/herself relative to the highway in a manner consistent with the...

49 CFR 325.37 - Location and operation of sound level measurement system; highway operations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... system; highway operations. 325.37 Section 325.37 Transportation Other Regulations Relating to...; Highway Operations § 325.37 Location and operation of sound level measurement system; highway operations..., the holder must orient himself/herself relative to the highway in a manner consistent with the...

14 CFR 125.325 - Instrument approach procedures and IFR landing minimums.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Instrument approach procedures and IFR landing minimums. 125.325 Section 125.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... make an instrument approach at an airport except in accordance with IFR weather minimums and unless the...

14 CFR 125.325 - Instrument approach procedures and IFR landing minimums.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Instrument approach procedures and IFR landing minimums. 125.325 Section 125.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... make an instrument approach at an airport except in accordance with IFR weather minimums and unless the...

14 CFR 125.325 - Instrument approach procedures and IFR landing minimums.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Instrument approach procedures and IFR landing minimums. 125.325 Section 125.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... make an instrument approach at an airport except in accordance with IFR weather minimums and unless the...

14 CFR 125.325 - Instrument approach procedures and IFR landing minimums.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Instrument approach procedures and IFR landing minimums. 125.325 Section 125.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... make an instrument approach at an airport except in accordance with IFR weather minimums and unless the...

14 CFR 125.325 - Instrument approach procedures and IFR landing minimums.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Instrument approach procedures and IFR landing minimums. 125.325 Section 125.325 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... make an instrument approach at an airport except in accordance with IFR weather minimums and unless the...

49 CFR 325.57 - Location and operation of sound level measurement systems; stationary test.

Code of Federal Regulations, 2010 CFR

2010-10-01

... the vehicle at an angle that is consistent with the recommendation of the system's manufacturer. If... systems; stationary test. 325.57 Section 325.57 Transportation Other Regulations Relating to...; Stationary Test § 325.57 Location and operation of sound level measurement systems; stationary test. (a) The...

12 CFR Appendix G to Part 1026 - Open-End Model Forms and Clauses

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 8 2013-01-01 2013-01-01 false Open-End Model Forms and Clauses G Appendix G...) Pt. 1026, App. G Appendix G to Part 1026—Open-End Model Forms and Clauses G-1Balance Computation Methods Model Clauses (Home-equity Plans) (§§ 1026.6 and 1026.7) G-1(A)Balance Computation Methods Model...

12 CFR Appendix G to Part 1026 - Open-End Model Forms and Clauses

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 8 2012-01-01 2012-01-01 false Open-End Model Forms and Clauses G Appendix G...) Pt. 1026, App. G Appendix G to Part 1026—Open-End Model Forms and Clauses G-1Balance Computation Methods Model Clauses (Home-equity Plans) (§§ 1026.6 and 1026.7) G-1(A)Balance Computation Methods Model...

12 CFR Appendix G to Part 1026 - Open-End Model Forms and Clauses

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 9 2014-01-01 2014-01-01 false Open-End Model Forms and Clauses G Appendix G...) Pt. 1026, App. G Appendix G to Part 1026—Open-End Model Forms and Clauses G-1Balance Computation Methods Model Clauses (Home-equity Plans) (§§ 1026.6 and 1026.7) G-1(A)Balance Computation Methods Model...

12 CFR Appendix G to Part 226 - Open-End Model Forms and Clauses

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 3 2012-01-01 2012-01-01 false Open-End Model Forms and Clauses G Appendix G... RESERVE SYSTEM TRUTH IN LENDING (REGULATION Z) Pt. 226, App. G Appendix G to Part 226—Open-End Model Forms and Clauses G-1Balance Computation Methods Model Clauses (Home-equity Plans) (§§ 226.6 and 226.7) G-1...

78 FR 17221 - Agency Information Collection Activities: Interagency Record of Request A, G, or NATO Dependent...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-20

...-0027] Agency Information Collection Activities: Interagency Record of Request A, G, or NATO Dependent Employment Authorization or Change/ Adjustment To/From A, G, or NATO Status, Form I-566; Revision of a... techniques or other forms of information technology, e.g., permitting electronic submission of responses...

Ultraviolet photorefraction at 325 nm in doped lithium niobate crystals

NASA Astrophysics Data System (ADS)

Xin, Feifei; Zhang, Guoquan; Bo, Fang; Sun, Haifeng; Kong, Yongfa; Xu, Jingjun; Volk, Tatyana; Rubinina, Natalia M.

2010-02-01

We studied the photorefractive effect of lithium niobate (LiNbO3) doped with Mg, Zn, In, Hf, or codoped with Mg and Fe at an ultraviolet (UV) wavelength down to 325 nm. It is found that the UV photorefraction of LiNbO3 doped with Mg, Zn, In, or Hf was enhanced significantly as compared to that of the nominally pure LiNbO3. Our results show that the property of resistance against photorefraction in highly Mg, Zn, In, or Hf doped LiNbO3 is true only in the visible and near-infrared wavelength range. By contrast, these crystals exhibit excellent photorefractive characteristics at UV wavelength of 325 nm, even better than those at 351 nm. For example, the photorefractive two-wave coupling gain coefficient Γ and the photorefractive recording sensitivity at 325 nm were measured to be ˜38 cm-1 and 37.7 cm/J, respectively, in a LiNbO3 crystal doped with 9 mol % Zn. The photorefractive response time of a Mg:LiNbO3 with a 9 mol % Mg was measured to be 73 ms with a total recording intensity of 614 mW/cm2 at 325 nm. In highly Mg, Zn, In, or Hf doped LiNbO3 crystals, diffusion dominates over photovoltaic effect and electrons are the dominant charge carriers in UV photorefraction at 325 nm. The results are also of interest to the study on the defect structure of LiNbO3 near to the absorption edge.

Antimicrobial Activity of Penicillin G and N-acetylcystein on Planktonic and Sessile Cells of Streptococcus suis.

PubMed

Espinosa, Ivette; Báez, Michel; Lobo, Evelyn; Martínez, Siomara; Gottschalk, Marcelo

2016-01-01

The aim of this study was to investigate the capacity of Streptococcus suis strains to form biofilms and to evaluate the antimicrobial activity of Penicillin G and N-acetylcystein (NAC) on both S. suis sessile and planktonic forms. Only non-typeable isolates of S. suis were correlated with a greater biofilm formation capacity. The MCI of Penicillin G and NAC required for inhibiting biofilm growth were higher than the required concentration for inhibiting planktonic growth. The combinations of NAC and Penicillin G showed a strong synergistic activity that inhibited biofilm formation and disrupted the pre-formed biofilm of S. suis.

12 CFR Appendix G to Part 226 - Open-End Model Forms and Clauses

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 3 2013-01-01 2013-01-01 false Open-End Model Forms and Clauses G Appendix G... RESERVE SYSTEM (CONTINUED) TRUTH IN LENDING (REGULATION Z) Pt. 226, App. G Appendix G to Part 226—Open-End Model Forms and Clauses G-1Balance Computation Methods Model Clauses (Home-equity Plans) (§§ 226.6 and...

21 CFR 172.325 - Bakers yeast protein.

Code of Federal Regulations, 2010 CFR

2010-04-01

... harmful microbial toxin. (d) The ingredient is used in food as a nutrient supplement as defined in § 170.3... Special Dietary and Nutritional Additives § 172.325 Bakers yeast protein. Bakers yeast protein may be...

21 CFR 172.325 - Bakers yeast protein.

Code of Federal Regulations, 2012 CFR

2012-04-01

... harmful microbial toxin. (d) The ingredient is used in food as a nutrient supplement as defined in § 170.3... Special Dietary and Nutritional Additives § 172.325 Bakers yeast protein. Bakers yeast protein may be...

21 CFR 172.325 - Bakers yeast protein.

Code of Federal Regulations, 2011 CFR

2011-04-01

... harmful microbial toxin. (d) The ingredient is used in food as a nutrient supplement as defined in § 170.3... Special Dietary and Nutritional Additives § 172.325 Bakers yeast protein. Bakers yeast protein may be...

21 CFR 172.325 - Bakers yeast protein.

Code of Federal Regulations, 2013 CFR

2013-04-01

... harmful microbial toxin. (d) The ingredient is used in food as a nutrient supplement as defined in § 170.3... Special Dietary and Nutritional Additives § 172.325 Bakers yeast protein. Bakers yeast protein may be...

Antibiosis by Pantoea agglomerans biocontrol strain E325 against Erwinia amylovora on apple blossom stigmas

USDA-ARS?s Scientific Manuscript database

Pantoea agglomerans E325, the active ingredient in a commercial product for fire blight control, was previously shown in vitro to produce a unique alkaline- and phosphate-sensitive antibiotic specific to Erwinia amylovora. Antibiosis was evaluated as a mode of antagonism on blossom stigmas using two...

46 CFR 148.325 - Wood chips; wood pellets; wood pulp pellets.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Wood chips; wood pellets; wood pulp pellets. 148.325... § 148.325 Wood chips; wood pellets; wood pulp pellets. (a) This part applies to wood chips and wood pulp... cargo hold. (b) No person may enter a cargo hold containing wood chips, wood pellets, or wood pulp...

46 CFR 148.325 - Wood chips; wood pellets; wood pulp pellets.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Wood chips; wood pellets; wood pulp pellets. 148.325... § 148.325 Wood chips; wood pellets; wood pulp pellets. (a) This part applies to wood chips and wood pulp... cargo hold. (b) No person may enter a cargo hold containing wood chips, wood pellets, or wood pulp...

46 CFR 148.325 - Wood chips; wood pellets; wood pulp pellets.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Wood chips; wood pellets; wood pulp pellets. 148.325... § 148.325 Wood chips; wood pellets; wood pulp pellets. (a) This part applies to wood chips and wood pulp... cargo hold. (b) No person may enter a cargo hold containing wood chips, wood pellets, or wood pulp...

46 CFR 148.325 - Wood chips; wood pellets; wood pulp pellets.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Wood chips; wood pellets; wood pulp pellets. 148.325... § 148.325 Wood chips; wood pellets; wood pulp pellets. (a) This part applies to wood chips and wood pulp... cargo hold. (b) No person may enter a cargo hold containing wood chips, wood pellets, or wood pulp...

Controlled release of Pantoea agglomerans E325 for biocontrol of fire blight

USDA-ARS?s Scientific Manuscript database

Microencapsulation and controlled release of Pantoea agglomerans strain E325 (E325), which is an antagonist to bacterial pathogen (Erwinia amylovora) of fire blight, a devastating disease of apple and pear, have been investigated. Uniform core-shell alginate microcapsules (AMCs), 60-300 µm in diamet...

33 CFR 325.3 - Public notice.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) The comment period based on § 325.2(d)(2); (15) A statement that any person may request, in writing... various evaluation factors on which decisions are based shall be included in every public notice. (1... whether to issue a permit will be based on an evaluation of the probable impact including cumulative...

33 CFR 325.3 - Public notice.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) The comment period based on § 325.2(d)(2); (15) A statement that any person may request, in writing... various evaluation factors on which decisions are based shall be included in every public notice. (1... whether to issue a permit will be based on an evaluation of the probable impact including cumulative...

33 CFR 325.3 - Public notice.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) The comment period based on § 325.2(d)(2); (15) A statement that any person may request, in writing... various evaluation factors on which decisions are based shall be included in every public notice. (1... whether to issue a permit will be based on an evaluation of the probable impact including cumulative...

33 CFR 325.3 - Public notice.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) The comment period based on § 325.2(d)(2); (15) A statement that any person may request, in writing... various evaluation factors on which decisions are based shall be included in every public notice. (1... whether to issue a permit will be based on an evaluation of the probable impact including cumulative...

33 CFR 325.3 - Public notice.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) The comment period based on § 325.2(d)(2); (15) A statement that any person may request, in writing... various evaluation factors on which decisions are based shall be included in every public notice. (1... whether to issue a permit will be based on an evaluation of the probable impact including cumulative...

Hemin/G-quadruplex structure and activity alteration induced by magnesium cations.

PubMed

Kosman, J; Juskowiak, B

2016-04-01

The influence of metal cations on G-quadruplex structure and peroxidase-mimicking DNAzyme activity was investigated. Experiments revealed a significant role of magnesium ion, which in the presence of potassium cation influenced DNAzyme activity. This ability has been associated with alteration of G-quadruplex topology and consequently affinity to bind hemin molecule. It has been demonstrated that G-quadruplex based on PS2.M sequence under these conditions formed parallel topology, which exhibited lower activity than that observed in standard potassium-containing solution. On the other hand DNAzyme/magnesium ion system based on telomeric sequence, which did not undergo significant structural changes, exhibited higher peroxidase activity upon magnesium ion addition. In both cases, the stabilization effect of magnesium cations on G-quadruplex structure was observed. The mechanism of DNAzyme activity alteration by magnesium ion can be explained by its influence on the pKa value of DNAzyme. Magnesium ion decreased pKa for PS2.M based system but increased it for telomeric DNAzyme. Magnesium cation effect on G-quadruplex structure as well as DNAzyme activity is particularly important since this ion is one of the most common metal cations in biological samples. Copyright © 2016 Elsevier B.V. All rights reserved.

Activation of Elongation Factor G by Phosphate Analogues

PubMed Central

Salsi, Enea; Farah, Elie

2016-01-01

EF-G is a universally conserved translational GTPase that promotes the translocation of tRNA and mRNA through the ribosome. EF-G binds to the ribosome in a GTP-bound form and subsequently catalyzes GTP hydrolysis. The contribution of the ribosome-stimulated GTP hydrolysis by EF-G to tRNA/mRNA translocation remains debated. Here, we show that while EF-G•GDP does not stably bind to the ribosome and induce translocation, EFG• GDP in complex with phosphate group analogues BeF3− and AlF4− promotes the translocation of tRNA and mRNA. Furthermore, the rates of mRNA translocation induced by EF-G in the presence of GTP and a non-hydrolysable analogue of GTP, GDP•BeF3−are similar. Our results are consistent with the model suggesting that GTP hydrolysis is not directly coupled to mRNA/tRNA translocation. Hence, GTP binding is required to induce the activated, translocation-competent conformation of EF-G while GTP hydrolysis triggers EF-G release from the ribosome. PMID:27063503

9 CFR 325.3 - Product transported within the United States as part of export movement.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Product transported within the United States as part of export movement. 325.3 Section 325.3 Animals and Animal Products FOOD SAFETY AND... PRODUCTS INSPECTION AND VOLUNTARY INSPECTION AND CERTIFICATION TRANSPORTATION § 325.3 Product transported...

25 CFR 32.5 - Evaluation of implementation of Pub. L. 95-561.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 25 Indians 1 2012-04-01 2011-04-01 true Evaluation of implementation of Pub. L. 95-561. 32.5... POLICIES § 32.5 Evaluation of implementation of Pub. L. 95-561. The Director, Office Indian Education Programs will develop guidelines for evaluating all functional and programmatic responsibilities associated...

25 CFR 32.5 - Evaluation of implementation of Pub. L. 95-561.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 25 Indians 1 2014-04-01 2014-04-01 false Evaluation of implementation of Pub. L. 95-561. 32.5... POLICIES § 32.5 Evaluation of implementation of Pub. L. 95-561. The Director, Office Indian Education Programs will develop guidelines for evaluating all functional and programmatic responsibilities associated...

25 CFR 32.5 - Evaluation of implementation of Pub. L. 95-561.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 25 Indians 1 2011-04-01 2011-04-01 false Evaluation of implementation of Pub. L. 95-561. 32.5... POLICIES § 32.5 Evaluation of implementation of Pub. L. 95-561. The Director, Office Indian Education Programs will develop guidelines for evaluating all functional and programmatic responsibilities associated...

25 CFR 32.5 - Evaluation of implementation of Pub. L. 95-561.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 25 Indians 1 2013-04-01 2013-04-01 false Evaluation of implementation of Pub. L. 95-561. 32.5... POLICIES § 32.5 Evaluation of implementation of Pub. L. 95-561. The Director, Office Indian Education Programs will develop guidelines for evaluating all functional and programmatic responsibilities associated...

Studies of G-quadruplexes formed within self-assembled DNA mini-circles.

PubMed

Klejevskaja, Beata; Pyne, Alice L B; Reynolds, Matthew; Shivalingam, Arun; Thorogate, Richard; Hoogenboom, Bart W; Ying, Liming; Vilar, Ramon

2016-10-13

We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.

49 CFR 325.23 - Type of measurement systems which may be used.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 5 2010-10-01 2010-10-01 false Type of measurement systems which may be used. 325... INTERSTATE MOTOR CARRIER NOISE EMISSION STANDARDS Instrumentation § 325.23 Type of measurement systems which may be used. The sound level measurement system must meet or exceed the requirements of American...

24 CFR 35.325 - Child with an environmental intervention blood lead level.

Code of Federal Regulations, 2012 CFR

2012-04-01

... intervention blood lead level. 35.325 Section 35.325 Housing and Urban Development Office of the Secretary... environmental intervention blood lead level. If a child less than 6 years of age living in a federally assisted dwelling unit has an environmental intervention blood lead level, the owner shall immediately conduct a...

24 CFR 35.325 - Child with an environmental intervention blood lead level.

Code of Federal Regulations, 2013 CFR

2013-04-01

... intervention blood lead level. 35.325 Section 35.325 Housing and Urban Development Office of the Secretary... environmental intervention blood lead level. If a child less than 6 years of age living in a federally assisted dwelling unit has an environmental intervention blood lead level, the owner shall immediately conduct a...

24 CFR 35.325 - Child with an environmental intervention blood lead level.

Code of Federal Regulations, 2011 CFR

2011-04-01

... intervention blood lead level. 35.325 Section 35.325 Housing and Urban Development Office of the Secretary... environmental intervention blood lead level. If a child less than 6 years of age living in a federally assisted dwelling unit has an environmental intervention blood lead level, the owner shall immediately conduct a...

24 CFR 35.325 - Child with an environmental intervention blood lead level.

Code of Federal Regulations, 2010 CFR

2010-04-01

... intervention blood lead level. 35.325 Section 35.325 Housing and Urban Development Office of the Secretary... environmental intervention blood lead level. If a child less than 6 years of age living in a federally assisted dwelling unit has an environmental intervention blood lead level, the owner shall immediately conduct a...

24 CFR 35.325 - Child with an environmental intervention blood lead level.

Code of Federal Regulations, 2014 CFR

2014-04-01

... intervention blood lead level. 35.325 Section 35.325 Housing and Urban Development Office of the Secretary... environmental intervention blood lead level. If a child less than 6 years of age living in a federally assisted dwelling unit has an environmental intervention blood lead level, the owner shall immediately conduct a...

76 FR 53907 - Determination That TALWIN COMPOUND (Aspirin; Pentazocine Hydrochloride) Tablets, 325 Milligrams...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-30

...] Determination That TALWIN COMPOUND (Aspirin; Pentazocine Hydrochloride) Tablets, 325 Milligrams; Equivalent to... determined that TALWIN COMPOUND (aspirin; pentazocine hydrochloride (HCl)) tablets, 325 milligrams (mg... determination will allow FDA to approve abbreviated new drug applications (ANDAs) for aspirin; pentazocine HCl...

Mechanism of inhibition of HIV-1 integrase by G-tetrad-forming oligonucleotides in Vitro.

PubMed

Jing, N; Marchand, C; Liu, J; Mitra, R; Hogan, M E; Pommier, Y

2000-07-14

The G-tetrad-forming oligonucleotides and have been identified as potent inhibitors of human immunodeficiency virus type 1 integrase (HIV-1 IN) activity (Rando, R. F., Ojwang, J., Elbaggari, A., Reyes, G. R., Tinder, R., McGrath, M. S., and Hogan, M. E. (1995) J. Biol. Chem. 270, 1754-1760; Mazumder, A., Neamati, N., Ojwang, J. O., Sunder, S., Rando, R. F., and Pommier, Y. (1996) Biochemistry 35, 13762-13771; Jing, N., and Hogan, M. E. (1998) J. Biol. Chem. 273, 34992-34999). To understand the inhibition of HIV-1 IN activity by the G-quartet inhibitors, we have designed the oligonucleotides and, composed of three and four G-quartets with stem lengths of 19 and 24 A, respectively. The fact that increasing the G-quartet stem length from 15 to 24 A kept inhibition of HIV-1 IN activity unchanged suggests that the binding interaction occurs between a GTGT loop domain of the G-quartet inhibitors and a catalytic site of HIV-1 IN, referred to as a face-to-face interaction. Docking the NMR structure of (Jing and Hogan (1998)) into the x-ray structure of the core domain of HIV-1 IN, HIV-1 IN-(51-209) (Maignan, S., Guilloteau, J.-P. , Qing, Z.-L., Clement-Mella, C., and Mikol, V. (1998) J. Mol. Biol. 282, 359-368), was performed using the GRAMM program. The statistical distributions of hydrogen bonding between HIV-1 IN and were obtained from the analyses of 1000 random docking structures. The docking results show a high probability of interaction between the GTGT loop residues of the G-quartet inhibitors and the catalytic site of HIV-1 IN, in agreement with the experimental observation.

A novel transition pathway of ligand-induced topological conversion from hybrid forms to parallel forms of human telomeric G-quadruplexes

PubMed Central

Wang, Zi-Fu; Li, Ming-Hao; Chen, Wei-Wen; Hsu, Shang-Te Danny; Chang, Ta-Chau

2016-01-01

The folding topology of DNA G-quadruplexes (G4s) depends not only on their nucleotide sequences but also on environmental factors and/or ligand binding. Here, a G4 ligand, 3,6-bis(1-methyl-4-vinylpyridium iodide)-9-(1-(1-methyl-piperidinium iodide)-3,6,9-trioxaundecane) carbazole (BMVC-8C3O), can induce topological conversion of non-parallel to parallel forms in human telomeric DNA G4s. Nuclear magnetic resonance (NMR) spectroscopy with hydrogen-deuterium exchange (HDX) reveals the presence of persistent imino proton signals corresponding to the central G-quartet during topological conversion of Tel23 and Tel25 G4s from hybrid to parallel forms, implying that the transition pathway mainly involves local rearrangements. In contrast, rapid HDX was observed during the transition of 22-CTA G4 from an anti-parallel form to a parallel form, resulting in complete disappearance of all the imino proton signals, suggesting the involvement of substantial unfolding events associated with the topological transition. Site-specific imino proton NMR assignments of Tel23 G4 enable determination of the interconversion rates of individual guanine bases and detection of the presence of intermediate states. Since the rate of ligand binding is much higher than the rate of ligand-induced topological conversion, a three-state kinetic model was evoked to establish the associated energy diagram for the topological conversion of Tel23 G4 induced by BMVC-8C3O. PMID:26975658

Novel anticancer agent, benzyldihydroxyoctenone, isolated from Streptomyces sp. causes G1 cell cycle arrest and induces apoptosis of HeLa cells.

PubMed

Lee, Chul-Hoon; Lim, Haeyoung; Moon, Sangik; Shin, Choonshik; Kim, Seunghyun; Kim, Bum-Joon; Lim, Yoongho

2007-06-01

In the course of screening for anticancer agents, a novel active compound, F3-2-5, was isolated from culture broth of Streptomyces sp., KACC91015. Its structure was identified using nuclear magnetic resonance, mass spectrometry, and molecular modeling experiments, and confirmed by total synthesis. The growth of various human cancer cell lines was inhibited in a dose-dependent manner by 0.06-0.48 mM F3-2-5 over 24 h. Its IC(50) values were estimated at 37 microM on HeLa, 72 microM on A549, and 190 microM on HT-29 cells. However, F3-2-5 had no antiproliferative effect on normal lymphocytes and normal fibroblasts used as controls. Moreover, it affected cell cycle regulation and caused apoptosis of the HeLa cells; chromatin condensation and DNA fragmentation were observed in cells exposed to 80 microM F3-2-5. Western blot analysis revealed that F3-2-5 inhibited phosphorylation of retinoblastoma protein (pRb) and reduced expression of cyclin-dependent kinase-4 and -6, and cyclin D1 and E, while levels of p53 and p21(WAF1/CIP1) increased. Taken together, these findings show that F3-2-5 inhibits proliferation of HeLa cells by inducing G(1) phase arrest as a consequence of inhibition of pRb phosphorylation following up-regulation of p21(WAF1/CIP1) and p53. Furthermore, apoptosis in HeLa cells treated with F3-2-5 was associated with an increase in Bax and p53, leading to release of cytochrome c, activation of caspase-3, and -8, and cleavage of poly (ADP-ribose) polymerase.

9 CFR 113.325 - Avian Encephalomyelitis Vaccine.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Avian Encephalomyelitis Vaccine. 113... REQUIREMENTS Live Virus Vaccines § 113.325 Avian Encephalomyelitis Vaccine. Avian Encephalomyelitis Vaccine... vaccine production. All serials shall be prepared from the first through the fifth passage from the Master...

9 CFR 113.325 - Avian Encephalomyelitis Vaccine.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Avian Encephalomyelitis Vaccine. 113... REQUIREMENTS Live Virus Vaccines § 113.325 Avian Encephalomyelitis Vaccine. Avian Encephalomyelitis Vaccine... vaccine production. All serials shall be prepared from the first through the fifth passage from the Master...

9 CFR 113.325 - Avian Encephalomyelitis Vaccine.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Avian Encephalomyelitis Vaccine. 113... REQUIREMENTS Live Virus Vaccines § 113.325 Avian Encephalomyelitis Vaccine. Avian Encephalomyelitis Vaccine... vaccine production. All serials shall be prepared from the first through the fifth passage from the Master...

9 CFR 113.325 - Avian Encephalomyelitis Vaccine.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Avian Encephalomyelitis Vaccine. 113... REQUIREMENTS Live Virus Vaccines § 113.325 Avian Encephalomyelitis Vaccine. Avian Encephalomyelitis Vaccine... vaccine production. All serials shall be prepared from the first through the fifth passage from the Master...

12 CFR 325.205 - Methodologies and practices.

Code of Federal Regulations, 2014 CFR

2014-01-01

... procedures, that are designed to ensure that its stress test processes satisfy the requirements in this... POLICY CAPITAL MAINTENANCE Annual Stress Test § 325.205 Methodologies and practices. (a) Potential impact on capital. In conducting a stress test under this subpart, during each quarter of the planning...

12 CFR 325.205 - Methodologies and practices.

Code of Federal Regulations, 2013 CFR

2013-01-01

... procedures, that are designed to ensure that its stress test processes satisfy the requirements in this... POLICY CAPITAL MAINTENANCE Annual Stress Test § 325.205 Methodologies and practices. (a) Potential impact on capital. In conducting a stress test under this subpart, during each quarter of the planning...

25 CFR 32.5 - Evaluation of implementation of Pub. L. 95-561.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Evaluation of implementation of Pub. L. 95-561. 32.5... POLICIES § 32.5 Evaluation of implementation of Pub. L. 95-561. The Director, Office Indian Education... with title XI of the Education Amendments of 1978 (Pub. L. 95-561), and in the January 1, 1981 annual...

Debio 0507 primarily forms diaminocyclohexane-Pt-d(GpG) and -d(ApG) DNA adducts in HCT116 cells

PubMed Central

King, C. L.; Ramachandran, S.; Collins, L.; Swenberg, J. A.; deKrafft, K. E.; Lin, W.; Cicurel, L.; Barbier, M.

2013-01-01

Purpose To characterize the cellular action mechanism of Debio 0507, we compared the major DNA adducts formed by Debio 0507- and oxaliplatin-treated HCT116 human colon carcinoma cells by a combination of inductively coupled plasma mass spectrometry (ICP-MS) and ultra-performance liquid chromatography mass spectrometry (UPLC-MS/MS). Methods HCT116 cells were treated with IC50 doses of Debio 0507 or oxaliplatin for 3 days. Total cellular Pt–DNA adducts were determined by ICP-MS. The DNA was digested, and the major Pt–DNA adducts formed by both drugs were characterized by UPLC/MS/MS essentially as described previously for cisplatin (Baskerville-Abraham et al. in Chem Res Toxicol 22:905–912, 2009). Results The Pt level/deoxynucleotide was 7.4/104 for DNA from Debio 0507-treated cells and 5.5/104 for oxaliplatin-treated cells following a 3-day treatment at the IC50 for each drug. UPLC-MS/MS in the positive ion mode confirmed the major Pt–DNA adducts formed by both drugs were dach-Pt-d(GpG) (904.2 m/z → 610 m/z and 904.2 m/z → 459 m/z) and dach-Pt-d(ApG) (888.2 m/z → 594 m/z and 888.2 m/z → 459 m/z). Conclusions These data show that the major DNA adducts formed by Debio 0507 are the dach-Pt-d(GpG) and dach-Pt-d(ApG) adducts and at equitoxic doses Debio 0507 and oxaliplatin form similar levels of dach-Pt-d(GpG) and dach-Pt-d(ApG) adducts. This suggests that the action mechanisms of Debio 0507 and oxaliplatin are similar at a cellular level. PMID:21968950

Debio 0507 primarily forms diaminocyclohexane-Pt-d(GpG) and -d(ApG) DNA adducts in HCT116 cells.

PubMed

King, C L; Ramachandran, S; Chaney, S G; Collins, L; Swenberg, J A; DeKrafft, K E; Lin, W; Cicurel, L; Barbier, M

2012-03-01

To characterize the cellular action mechanism of Debio 0507, we compared the major DNA adducts formed by Debio 0507- and oxaliplatin-treated HCT116 human colon carcinoma cells by a combination of inductively coupled plasma mass spectrometry (ICP-MS) and ultraperformance liquid chromatography mass spectrometry (UPLC-MS/MS). HCT116 cells were treated with IC(50) doses of Debio 0507 or oxaliplatin for 3 days. Total cellular Pt-DNA adducts were determined by ICP-MS. The DNA was digested, and the major Pt-DNA adducts formed by both drugs were characterized by UPLC/MS/MS essentially as described previously for cisplatin (Baskerville-Abraham et al. in Chem Res Toxicol 22:905-912, 2009). The Pt level/deoxynucleotide was 7.4/10(4) for DNA from Debio 0507-treated cells and 5.5/10(4) for oxaliplatin-treated cells following a 3-day treatment at the IC(50) for each drug. UPLC-MS/MS in the positive ion mode confirmed the major Pt-DNA adducts formed by both drugs were dach-Pt-d(GpG) (904.2 m/z → 610 m/z and 904.2 m/z → 459 m/z) and dach-Pt-d(ApG) (888.2 m/z → 594 m/z and 888.2 m/z → 459 m/z). These data show that the major DNA adducts formed by Debio 0507 are the dach-Pt-d(GpG) and dach-Pt-d(ApG) adducts and at equitoxic doses Debio 0507 and oxaliplatin form similar levels of dach-Pt-d(GpG) and dach-Pt-d(ApG) adducts. This suggests that the action mechanisms of Debio 0507 and oxaliplatin are similar at a cellular level.

20 CFR 641.325 - What information must be provided in the State Plan?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false What information must be provided in the State Plan? 641.325 Section 641.325 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR PROVISIONS GOVERNING THE SENIOR COMMUNITY SERVICE EMPLOYMENT PROGRAM The State Senior...

Professionalism as a Form of Work Commitment.

ERIC Educational Resources Information Center

Morrow, Paula C.; Goetz, Joe F., Jr.

1988-01-01

Evaluated concept of professionalism as a form of work commitment using a modified version of Hall's (1968) multidimensional measure of professionalism on 325 accountants in public practice. Examined three forms of work commitment--job involvement, organizational commitment, and work ethic endorsement--and four professional behaviors. Suggests the…

75 FR 11192 - Agency Information Collection Activities: Form I-730, Revision of an Existing Information...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-10

...-0037] Agency Information Collection Activities: Form I-730, Revision of an Existing Information Collection Request; Comment Request ACTION: 60-Day Notice of Information Collection under Review: Form I- 730..., mechanical, or other technological collection techniques, or other forms of information technology, e.g...

9 CFR 325.11 - Inedible articles: denaturing and other means of identification; exceptions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... other means of identification; exceptions. 325.11 Section 325.11 Animals and Animal Products FOOD SAFETY... the carcasses of livestock, or other meat food product, that has not been inspected and passed at an..., and no carcass, part of a carcass, fat or other meat food product that is adulterated or misbranded...

9 CFR 325.11 - Inedible articles: denaturing and other means of identification; exceptions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... other means of identification; exceptions. 325.11 Section 325.11 Animals and Animal Products FOOD SAFETY... the carcasses of livestock, or other meat food product, that has not been inspected and passed at an..., and no carcass, part of a carcass, fat or other meat food product that is adulterated or misbranded...

9 CFR 325.11 - Inedible articles: denaturing and other means of identification; exceptions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... other means of identification; exceptions. 325.11 Section 325.11 Animals and Animal Products FOOD SAFETY... the carcasses of livestock, or other meat food product, that has not been inspected and passed at an..., and no carcass, part of a carcass, fat or other meat food product that is adulterated or misbranded...

9 CFR 325.11 - Inedible articles: denaturing and other means of identification; exceptions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... other means of identification; exceptions. 325.11 Section 325.11 Animals and Animal Products FOOD SAFETY... the carcasses of livestock, or other meat food product, that has not been inspected and passed at an..., and no carcass, part of a carcass, fat or other meat food product that is adulterated or misbranded...

9 CFR 325.11 - Inedible articles: denaturing and other means of identification; exceptions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... other means of identification; exceptions. 325.11 Section 325.11 Animals and Animal Products FOOD SAFETY... the carcasses of livestock, or other meat food product, that has not been inspected and passed at an..., and no carcass, part of a carcass, fat or other meat food product that is adulterated or misbranded...

Tracking G-protein-coupled receptor activation using genetically encoded infrared probes.

PubMed

Ye, Shixin; Zaitseva, Ekaterina; Caltabiano, Gianluigi; Schertler, Gebhard F X; Sakmar, Thomas P; Deupi, Xavier; Vogel, Reiner

2010-04-29

Rhodopsin is a prototypical heptahelical family A G-protein-coupled receptor (GPCR) responsible for dim-light vision. Light isomerizes rhodopsin's retinal chromophore and triggers concerted movements of transmembrane helices, including an outward tilting of helix 6 (H6) and a smaller movement of H5, to create a site for G-protein binding and activation. However, the precise temporal sequence and mechanism underlying these helix rearrangements is unclear. We used site-directed non-natural amino acid mutagenesis to engineer rhodopsin with p-azido-l-phenylalanine residues incorporated at selected sites, and monitored the azido vibrational signatures using infrared spectroscopy as rhodopsin proceeded along its activation pathway. Here we report significant changes in electrostatic environments of the azido probes even in the inactive photoproduct Meta I, well before the active receptor state was formed. These early changes suggest a significant rotation of H6 and movement of the cytoplasmic part of H5 away from H3. Subsequently, a large outward tilt of H6 leads to opening of the cytoplasmic surface to form the active receptor photoproduct Meta II. Thus, our results reveal early conformational changes that precede larger rigid-body helix movements, and provide a basis to interpret recent GPCR crystal structures and to understand conformational sub-states observed during the activation of other GPCRs.

Tauroursodeoxycholic acid binds to the G-protein site on light activated rhodopsin.

PubMed

Lobysheva, E; Taylor, C M; Marshall, G R; Kisselev, O G

2018-05-01

The heterotrimeric G-protein binding site on G-protein coupled receptors remains relatively unexplored regarding its potential as a new target of therapeutic intervention or as a secondary site of action by the existing drugs. Tauroursodeoxycholic acid bears structural resemblance to several compounds that were previously identified to specifically bind to the light-activated form of the visual receptor rhodopsin and to inhibit its activation of transducin. We show that TUDCA stabilizes the active form of rhodopsin, metarhodopsin II, and does not display the detergent-like effects of common amphiphilic compounds that share the cholesterol scaffold structure, such as deoxycholic acid. Computer docking of TUDCA to the model of light-activated rhodopsin revealed that it interacts using similar mode of binding to the C-terminal domain of transducin alpha subunit. The ring regions of TUDCA made hydrophobic contacts with loop 3 region of rhodopsin, while the tail of TUDCA is exposed to solvent. The results show that TUDCA interacts specifically with rhodopsin, which may contribute to its wide-ranging effects on retina physiology and as a potential therapeutic compound for retina degenerative diseases. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

29 CFR 778.325 - Effect on salary covering more than 40 hours' pay.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Effect on salary covering more than 40 hours' pay. 778.325... COMPENSATION Special Problems Reduction in Workweek Schedule with No Change in Pay § 778.325 Effect on salary covering more than 40 hours' pay. The same reasoning applies to salary covering straight time pay for a...

9 CFR 325.6 - Shipment of paunches between official establishments under official seal; certificate.

Code of Federal Regulations, 2011 CFR

2011-01-01

... establishments under official seal; certificate. 325.6 Section 325.6 Animals and Animal Products FOOD SAFETY AND... between official establishments under official seal; certificate. Cattle and sheep paunches which have... seal of the Department as prescribed in § 312.5(a) of this subchapter. ...

9 CFR 325.6 - Shipment of paunches between official establishments under official seal; certificate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... establishments under official seal; certificate. 325.6 Section 325.6 Animals and Animal Products FOOD SAFETY AND... between official establishments under official seal; certificate. Cattle and sheep paunches which have... seal of the Department as prescribed in § 312.5(a) of this subchapter. ...

Mini G protein probes for active G protein-coupled receptors (GPCRs) in live cells.

PubMed

Wan, Qingwen; Okashah, Najeah; Inoue, Asuka; Nehmé, Rony; Carpenter, Byron; Tate, Christopher G; Lambert, Nevin A

2018-05-11

G protein-coupled receptors (GPCRs) are key signaling proteins that regulate nearly every aspect of cell function. Studies of GPCRs have benefited greatly from the development of molecular tools to monitor receptor activation and downstream signaling. Here, we show that mini G proteins are robust probes that can be used in a variety of assay formats to report GPCR activity in living cells. Mini G (mG) proteins are engineered GTPase domains of Gα subunits that were developed for structural studies of active-state GPCRs. Confocal imaging revealed that mG proteins fused to fluorescent proteins were located diffusely in the cytoplasm and translocated to sites of receptor activation at the cell surface and at intracellular organelles. Bioluminescence resonance energy transfer (BRET) assays with mG proteins fused to either a fluorescent protein or luciferase reported agonist, superagonist, and inverse agonist activities. Variants of mG proteins (mGs, mGsi, mGsq, and mG12) corresponding to the four families of Gα subunits displayed appropriate coupling to their cognate GPCRs, allowing quantitative profiling of subtype-specific coupling to individual receptors. BRET between luciferase-mG fusion proteins and fluorescent markers indicated the presence of active GPCRs at the plasma membrane, Golgi apparatus, and endosomes. Complementation assays with fragments of NanoLuc luciferase fused to GPCRs and mG proteins reported constitutive receptor activity and agonist-induced activation with up to 20-fold increases in luminescence. We conclude that mG proteins are versatile tools for studying GPCR activation and coupling specificity in cells and should be useful for discovering and characterizing G protein subtype-biased ligands. © 2018 Wan et al.

49 CFR 325.57 - Location and operation of sound level measurement systems; stationary test.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Location and operation of sound level measurement systems; stationary test. 325.57 Section 325.57 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL REGULATIONS COMPLIANCE WITH INTERSTATE MOTOR...

49 CFR 325.37 - Location and operation of sound level measurement system; highway operations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... recommendation of the manufacturer of the sound level measurement system. (2) In no case shall the holder or... angle that is consistent with the recommendation of the system's manufacturer. If the manufacturer of... system; highway operations. 325.37 Section 325.37 Transportation Other Regulations Relating to...

7 CFR 3017.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-01-01

... knowingly do business with an excluded person, we may disallow costs, annul or terminate the transaction... in a covered transaction? 3017.325 Section 3017.325 Agriculture Regulations of the Department of... DEBARMENT AND SUSPENSION (NONPROCUREMENT) Responsibilities of Participants Regarding Transactions § 3017.325...

Membrane-localized extra-large G proteins and Gbg of the heterotrimeric G proteins form functional complexes engaged in plant immunity in Arabidopsis.

PubMed

Maruta, Natsumi; Trusov, Yuri; Brenya, Eric; Parekh, Urvi; Botella, José Ramón

2015-03-01

In animals, heterotrimeric G proteins, comprising Ga, Gb, and Gg subunits, are molecular switches whose function tightly depends on Ga and Gbg interaction. Intriguingly, in Arabidopsis (Arabidopsis thaliana), multiple defense responses involve Gbg, but not Ga. We report here that the Gbg dimer directly partners with extra-large G proteins (XLGs) to mediate plant immunity. Arabidopsis mutants deficient in XLGs, Gb, and Gg are similarly compromised in several pathogen defense responses, including disease development and production of reactive oxygen species. Genetic analysis of double, triple, and quadruple mutants confirmed that XLGs and Gbg functionally interact in the same defense signaling pathways. In addition, mutations in XLG2 suppressed the seedling lethal and cell death phenotypes of BRASSINOSTEROID INSENSITIVE1-associated receptor kinase1-interacting receptor-like kinase1 mutants in an identical way as reported for Arabidopsis Gb-deficient mutants. Yeast (Saccharomyces cerevisiae) three-hybrid and bimolecular fluorescent complementation assays revealed that XLG2 physically interacts with all three possible Gbg dimers at the plasma membrane. Phylogenetic analysis indicated a close relationship between XLGs and plant Ga subunits, placing the divergence point at the dawn of land plant evolution. Based on these findings, we conclude that XLGs form functional complexes with Gbg dimers, although the mechanism of action of these complexes, including activation/deactivation, must be radically different form the one used by the canonical Ga subunit and are not likely to share the same receptors. Accordingly, XLGs expand the repertoire of heterotrimeric G proteins in plants and reveal a higher level of diversity in heterotrimeric G protein signaling.

Sequences with high propensity to form G-quartet structures in kinetoplast DNA from Phytomonas serpens.

PubMed

Sá-Carvalho, D; Traub-Cseko, Y M

1995-06-01

Naturally occurring sequences containing repetitive guanine motifs have the potential to form tetraplex DNA. Phytomonas serpens minicircle DNA shows some regions where one strand is composed mainly of G and T (GT regions). These regions contain several stretches of contiguous guanines. An oligonucleotide was constructed with the sequence corresponding to one of these regions (Phyto-GT). It was demonstrated by native gel electrophoresis and methylation protection that Phyto-GT forms tetramolecular (G4), bimolecular (G'2) and unimolecular (G4') structures stabilized through G-quartets. Tetraplex DNA formation by this sequence could have biological relevance as it can be formed in physiological conditions and GT regions comprise approximately one-third of P. serpens and Crithidia oncopelti minicircles.

29 CFR 1471.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-07-01

... business with an excluded person, we may disallow costs, annul or terminate the transaction, issue a stop... covered transaction? 1471.325 Section 1471.325 Labor Regulations Relating to Labor (Continued) FEDERAL... of Participants Regarding Transactions Doing Business with Other Persons § 1471.325 What happens if I...

24 CFR 964.325 - Notice of funding availability.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Notice of funding availability. 964... Centers (FIC) Program § 964.325 Notice of funding availability. A Notice of Funding Availability will be published periodically in the Federal Register containing the amounts of funds available, funding criteria...

9 CFR 325.18 - Diverting of shipments, breaking of seals, and reloading by carrier in emergency; reporting to...

Code of Federal Regulations, 2010 CFR

2010-01-01

... seals, and reloading by carrier in emergency; reporting to Regional Director. 325.18 Section 325.18... CERTIFICATION TRANSPORTATION § 325.18 Diverting of shipments, breaking of seals, and reloading by carrier in...) In case of wreck or similar extraordinary emergency, the Department seals on a railroad car or other...

2 CFR 180.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-01-01

... person, the Federal agency responsible for your transaction may disallow costs, annul or terminate the... excluded person in a covered transaction? 180.325 Section 180.325 Grants and Agreements OFFICE OF... Regarding Transactions Doing Business With Other Persons § 180.325 What happens if I do business with an...

22 CFR 1508.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-04-01

... person, we may disallow costs, annul or terminate the transaction, issue a stop work order, debar or... person in a covered transaction? 1508.325 Section 1508.325 Foreign Relations AFRICAN DEVELOPMENT... Regarding Transactions Doing Business with Other Persons § 1508.325 What happens if I do business with an...

49 CFR 325.37 - Location and operation of sound level measurement system; highway operations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Location and operation of sound level measurement...; Highway Operations § 325.37 Location and operation of sound level measurement system; highway operations. (a) The microphone of a sound level measurement system that conforms to the rules in § 325.23 of this...

12 CFR Appendix G to Part 226 - Open-End Model Forms and Clauses

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 3 2014-01-01 2014-01-01 false Open-End Model Forms and Clauses G Appendix G... RESERVE SYSTEM (CONTINUED) TRUTH IN LENDING (REGULATION Z) Pt. 226, App. G Appendix G to Part 226—Open-End... Card Accounts Under an Open-End (Not Home-Secured) Consumer Credit Plan [Interest will be charged to...

77 FR 9259 - Agency Information Collection Activities: Forms G-1041 and G-1041A, Extension of a Currently...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-16

..., Genealogy Index Search Request and Genealogy Records Request. The Department of Homeland Security, U.S... the Forms/Collections: Genealogy Index Search Request and Genealogy Records Request. (3) Agency form.... USCIS will use these forms will to facilitate an accurate and timely response to genealogy index search...

Platelet Inhibition by 81 and 325 mg Aspirin Daily in Men vs. Women without Clinically Apparent Cardiovascular Disease

PubMed Central

Qayyum, Rehan; Becker, Diane M.; Yanek, Lisa R.; Moy, Taryn F.; Becker, Lewis C.; Faraday, Nauder; Vaidya, Dhananjay

2011-01-01

Compared to men, women have greater platelet aggregation before and after low-dose aspirin. It is not known whether high-dose aspirin therapy brings residual platelet aggregation in women closer to men. Our objective was to compare the inhibition of platelet aggregation in women and men after low and high-dose aspirin. We enrolled healthy subjects (N=106) in a trial of 14 days of aspirin 81 mg/day followed by 14 days of 325 mg/day. Platelet function was measured at baseline and following both aspirin doses. Women had greater baseline platelet activation measures. After both aspirin doses, both sexes had near complete suppression of platelet aggregation to arachidonic acid in whole blood and in platelet-rich plasma (PRP), the direct cyclooxygenase-1 (COX-1) pathway affected by aspirin. For indirect pathways, women had significantly greater residual platelet activation to collagen and adenosine diphosphate (ADP) in whole blood after both aspirin doses and in response to collagen and ADP in PRP after aspirin 325 mg/day only. After aspirin 325 mg/day, women continued to have greater residual platelet aggregation compared to men after aspirin 81 mg/day in response to collagen (p=0.016 in whole blood and p=0.037 in PRP), ADP (p<0.001 in whole blood and p=0.012 in PRP), and epinephrine (p=0.03 in PRP). Excretion of urinary thromboxane metabolite (urinary 11-dehydro thromboxane B2) decreased after aspirin to a similar extent in both sexes. In conclusion, women continue to have greater residual platelet activity after high-dose aspirin even when compared to men treated with a lower dose of aspirin. PMID:18435972

34 CFR 85.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-07-01

... person, we may disallow costs, annul or terminate the transaction, issue a stop work order, debar or... in a covered transaction? 85.325 Section 85.325 Education Office of the Secretary, Department of... Regarding Transactions Doing Business with Other Persons § 85.325 What happens if I do business with an...

Potential of osmoadaptation for improving Pantoea agglomerans E325 as biocontrol agent for fire blight of apple and pear

USDA-ARS?s Scientific Manuscript database

Pantoea agglomerans biocontrol strain E325 is the active ingredient in a commercial product for fire blight, a destructive disease of apple and pear initiated by Erwinia amylovora in flowers. Osmoadaptation, involving the combination of saline osmotic stress and osmolyte amendment to growth media, w...

49 CFR 325.57 - Location and operation of sound level measurement systems; stationary test.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 5 2012-10-01 2012-10-01 false Location and operation of sound level measurement...; Stationary Test § 325.57 Location and operation of sound level measurement systems; stationary test. (a) The microphone of a sound level measurement system that conforms to the rules in § 325.23 shall be located at a...

49 CFR 325.57 - Location and operation of sound level measurement systems; stationary test.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Location and operation of sound level measurement...; Stationary Test § 325.57 Location and operation of sound level measurement systems; stationary test. (a) The microphone of a sound level measurement system that conforms to the rules in § 325.23 shall be located at a...

49 CFR 325.57 - Location and operation of sound level measurement systems; stationary test.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Location and operation of sound level measurement...; Stationary Test § 325.57 Location and operation of sound level measurement systems; stationary test. (a) The microphone of a sound level measurement system that conforms to the rules in § 325.23 shall be located at a...

21 CFR 1404.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-04-01

... person, we may disallow costs, annul or terminate the transaction, issue a stop work order, debar or... person in a covered transaction? 1404.325 Section 1404.325 Food and Drugs OFFICE OF NATIONAL DRUG CONTROL... Regarding Transactions Doing Business with Other Persons § 1404.325 What happens if I do business with an...

22 CFR 208.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false What happens if I do business with an excluded person in a covered transaction? 208.325 Section 208.325 Foreign Relations AGENCY FOR INTERNATIONAL... Regarding Transactions Doing Business with Other Persons § 208.325 What happens if I do business with an...

22 CFR 208.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false What happens if I do business with an excluded person in a covered transaction? 208.325 Section 208.325 Foreign Relations AGENCY FOR INTERNATIONAL... Regarding Transactions Doing Business with Other Persons § 208.325 What happens if I do business with an...

E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pruneda, Jonathan N.; Smith, F. Donelson; Daurie, Angela

Pathogenic bacteria introduce effector proteins directly into the cytosol of eukaryotic cells to promote invasion and colonization. OspG, a Shigella spp. effector kinase, plays a role in this process by helping to suppress the host inflammatory response. OspG has been reported to bind host E2 ubiquitin-conjugating enzymes activated with ubiquitin (E2~Ub), a key enzyme complex in ubiquitin transfer pathways. A cocrystal structure of the OspG/UbcH5c~Ub complex reveals that complex formation has important ramifications for the activity of both OspG and the UbcH5c~Ub conjugate. OspG is a minimal kinase domain containing only essential elements required for catalysis. UbcH5c~Ub binding stabilizes anmore » active conformation of the kinase, greatly enhancing OspG kinase activity. In contrast, interaction with OspG stabilizes an extended, less reactive form of UbcH5c~Ub. Recognizing conserved E2 features, OspG can interact with at least ten distinct human E2s~Ub. Mouse oral infection studies indicate that E2~Ub conjugates act as novel regulators of OspG effector kinase function in eukaryotic host cells.« less

12 CFR 325.101 - Authority, purpose, scope, other supervisory authority, and disclosure of capital categories.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Authority, purpose, scope, other supervisory authority, and disclosure of capital categories. 325.101 Section 325.101 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY CAPITAL MAINTENANCE Prompt Corrective...

9 CFR 325.5 - Unmarked inspected product transported under official seal between official establishments for...

Code of Federal Regulations, 2010 CFR

2010-01-01

... under official seal between official establishments for further processing; certificate. 325.5 Section... CERTIFICATION TRANSPORTATION § 325.5 Unmarked inspected product transported under official seal between official... other means of conveyance which is sealed by a Program employee with an official seal of the Department...

9 CFR 325.5 - Unmarked inspected product transported under official seal between official establishments for...

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Unmarked inspected product transported under official seal between official establishments for further processing; certificate. 325.5 Section 325.5 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY...

10 CFR 431.325 - Units to be tested.

Code of Federal Regulations, 2011 CFR

2011-01-01

... EQUIPMENT Metal Halide Lamp Ballasts and Fixtures Test Procedures § 431.325 Units to be tested. For each basic model of metal halide lamp ballast selected for testing, a sample of sufficient size, no less than... energy efficiency calculated as the measured output power to the lamp divided by the measured input power...

24 CFR 891.325 - Lead-based paint requirements.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 4 2014-04-01 2014-04-01 false Lead-based paint requirements. 891... Section 811 Supportive Housing for Persons With Disabilities § 891.325 Lead-based paint requirements. The requirements of the Lead-Based Paint Poisoning Prevention Act (42 U.S.C. 4821-4846), the Residential Lead-Based...

24 CFR 891.325 - Lead-based paint requirements.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 4 2013-04-01 2013-04-01 false Lead-based paint requirements. 891... Section 811 Supportive Housing for Persons With Disabilities § 891.325 Lead-based paint requirements. The requirements of the Lead-Based Paint Poisoning Prevention Act (42 U.S.C. 4821-4846), the Residential Lead-Based...

24 CFR 891.325 - Lead-based paint requirements.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Lead-based paint requirements. 891... Section 811 Supportive Housing for Persons With Disabilities § 891.325 Lead-based paint requirements. The requirements of the Lead-Based Paint Poisoning Prevention Act (42 U.S.C. 4821-4846), the Residential Lead-Based...

24 CFR 891.325 - Lead-based paint requirements.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 4 2011-04-01 2011-04-01 false Lead-based paint requirements. 891... Section 811 Supportive Housing for Persons With Disabilities § 891.325 Lead-based paint requirements. The requirements of the Lead-Based Paint Poisoning Prevention Act (42 U.S.C. 4821-4846), the Residential Lead-Based...

24 CFR 891.325 - Lead-based paint requirements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Lead-based paint requirements. 891... Section 811 Supportive Housing for Persons With Disabilities § 891.325 Lead-based paint requirements. The requirements of the Lead-Based Paint Poisoning Prevention Act (42 U.S.C. 4821-4846), the Residential Lead-Based...

47 CFR 1.1705 - Forms; electronic and manual filing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... with the basic signal leakage performance criteria. (2) FCC Form 321, Aeronautical Frequency... time the data entry is made. (2) Attachments to applications must be uploaded along with the...) Forms 320, 321, 322, 324, 325, and 327 may be filed manually. (2) Manual filings must be submitted to...

47 CFR 1.1705 - Forms; electronic and manual filing.

Code of Federal Regulations, 2011 CFR

2011-10-01

... with the basic signal leakage performance criteria. (2) FCC Form 321, Aeronautical Frequency... time the data entry is made. (2) Attachments to applications must be uploaded along with the...) Forms 320, 321, 322, 324, 325, and 327 may be filed manually. (2) Manual filings must be submitted to...

31 CFR 19.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2012 CFR

2012-07-01

... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false What happens if I do business with an excluded person in a covered transaction? 19.325 Section 19.325 Money and Finance: Treasury Office of the... Participants Regarding Transactions Doing Business with Other Persons § 19.325 What happens if I do business...

31 CFR 19.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false What happens if I do business with an excluded person in a covered transaction? 19.325 Section 19.325 Money and Finance: Treasury Office of the... Participants Regarding Transactions Doing Business with Other Persons § 19.325 What happens if I do business...

31 CFR 19.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false What happens if I do business with an excluded person in a covered transaction? 19.325 Section 19.325 Money and Finance: Treasury Office of the... Participants Regarding Transactions Doing Business with Other Persons § 19.325 What happens if I do business...

31 CFR 19.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false What happens if I do business with an excluded person in a covered transaction? 19.325 Section 19.325 Money and Finance: Treasury Office of the... Participants Regarding Transactions Doing Business with Other Persons § 19.325 What happens if I do business...

31 CFR 19.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false What happens if I do business with an excluded person in a covered transaction? 19.325 Section 19.325 Money and Finance: Treasury Office of the... Participants Regarding Transactions Doing Business with Other Persons § 19.325 What happens if I do business...

75 FR 6849 - Airworthiness Directives; Airbus Model A310-221, -222, -322, -324, and -325 Airplanes, and Model...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-12

... Airworthiness Directives; Airbus Model A310-221, -222, -322, - 324, and -325 Airplanes, and Model A300 B4-620... applies to Airbus Model A310-221, -222, -322, -324, and -325 airplanes, and Model A300 B4-620, B4-622, B4... Model A310-221, - 222, -322, -324, and -325 airplanes), both including Appendix 1, both dated October 3...

Star formation activity in the southern Galactic H II region G351.63-1.25

NASA Astrophysics Data System (ADS)

Vig, S.; Ghosh, S. K.; Ojha, D. K.; Verma, R. P.; Tamura, M.

2014-06-01

The southern Galactic high-mass star-forming region, G351.63-1.25, is an H II region-molecular cloud complex with a luminosity of ˜2.0 × 105 L⊙, located at a distance of 2.4 kpc from the Sun. In this paper, we focus on the investigation of the associated H II region, embedded cluster and the interstellar medium in the vicinity of G351.63-1.25. We address the identification of exciting source(s) as well as the census of the stellar populations, in an attempt to unfold star formation activity in this region. The ionized gas distribution has been mapped using the Giant Metrewave Radio Telescope, India, at three frequencies: 1280, 610 and 325 MHz. The H II region shows an elongated morphology and the 1280 MHz map comprises six resolved high-density regions encompassed by diffuse emission spanning 1.4 × 1.0 pc2. Based on the measurements of flux densities at multiple radio frequencies, the brightest ultracompact core has electron temperature Te˜7647 {±} 153 K and emission measure, EM˜2.0 {±} 0.8×107 cm-6 pc. The zero-age main-sequence spectral type of the brightest radio core is O7.5. We have carried out near-infrared observations in the JHKs bands using the SIRIUS camera on the 1.4 m Infrared Survey Facility telescope. The near-infrared images reveal the presence of a cluster embedded in nebulous fan-shaped emission. The log-normal slope of the K-band luminosity function of the embedded cluster is found to be ˜0.27 ± 0.03, and the fraction of the near-infrared excess stars is estimated to be 43 per cent. These indicate that the age of the cluster is consistent with ˜1 Myr. Other available data of this region show that the warm (mid-infrared) and cold (millimetre) dust emission peak at different locations indicating progressive stages of star formation process. The champagne flow model from a flat, thin molecular cloud is used to explain the morphology of radio emission with respect to the millimetre cloud and infrared brightness.

41 CFR 102-117.325 - May my agency appear on its own behalf before a transportation regulatory body proceeding?

Code of Federal Regulations, 2011 CFR

2011-01-01

... its own behalf before a transportation regulatory body proceeding? 102-117.325 Section 102-117.325... Regulatory Body Proceedings § 102-117.325 May my agency appear on its own behalf before a transportation regulatory body proceeding? Generally, no executive agency may appear on its own behalf in any proceeding...

41 CFR 102-117.325 - May my agency appear on its own behalf before a transportation regulatory body proceeding?

Code of Federal Regulations, 2010 CFR

2010-07-01

... its own behalf before a transportation regulatory body proceeding? 102-117.325 Section 102-117.325... Regulatory Body Proceedings § 102-117.325 May my agency appear on its own behalf before a transportation regulatory body proceeding? Generally, no executive agency may appear on its own behalf in any proceeding...

Antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi.

PubMed

Felczykowska, Agnieszka; Pastuszak-Skrzypczak, Alicja; Pawlik, Anna; Bogucka, Krystyna; Herman-Antosiewicz, Anna; Guzow-Krzemińska, Beata

2017-06-07

Lichens that were used in traditional medicine for ages produce numerous secondary metabolites, however our knowledge about biological activities of substances secreted by separated bionts is scarce. The main objectives of this study were to isolate and find optimal conditions for the growth of mycelia from three common lichen-forming fungi, i.e. Caloplaca pusilla, Protoparmeliopsis muralis and Xanthoria parietina and to evaluate antibacterial and antiproliferative activities of their acetone extracts. Agar disc diffusion and broth microdilution methods were used to test antimicrobial activity against six species of bacteria. MTT method, flow cytometry assay and DAPI staining were applied to test antiproliferative activity of selected extracts against MCF-7 (human breast adenocarcinoma), PC-3 (human prostate cancer) and HeLa (human cervix adenocarcinoma) cancer cells. P. muralis strongly inhibited the growth of Gram-positive bacteria, i.e. Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis (MICs from 6.67 to 100.00 μg mL -1 ). X. parietina grown on PDA and G-LBM media decreased HeLa or MCF-7 cancer cells viability with IC 50 values of about 8 μg mL -1 , while C. pusilla grown on G-LBM medium showed the highest potency in decreasing MCF-7 (7.29 μg mL -1 ), PC-3 (7.96 μg mL -1 ) and HeLa (6.57 μg mL -1 ) cancer cells viability. We also showed induction of apoptosis in HeLa, PC-3 and MCF-7 cell lines treated with increasing concentrations of C. pusilla extract. We showed that selected acetone extracts demonstrated a strong antimicrobial and anticancer effects that suggests that aposymbiotically cultured lichen-forming fungi can be a source of antibacterial and antiproliferative compounds.

Influence of decreased fibrinolytic activity and plasminogen activator inhibitor-1 4G/5G polymorphism on the risk of venous thrombosis.

PubMed

Vuckovic, Biljana A; Djeric, Mirjana J; Tomic, Branko V; Djordjevic, Valentina J; Bajkin, Branislav V; Mitic, Gorana P

2018-01-01

: Objective of our study is to determine whether decreased fibrinolytic activity or plasminogen activator inhibitor (PAI)-1 4G/5G polymorphism influence the risk of venous thrombosis.Our case-control study included 100 patients with venous thrombosis, and 100 random controls. When patients were compared with random controls, unconditional logistic regression was used to calculate odds ratios (ORs) with 95% confidence intervals (CIs).Decreased fibrinolytic activity yielded a 2.7-fold increase in risk for venous thrombosis than physiological fibrinolytic activity (OR 2.70; 95% CI 1.22-5.98), when comparing patients with random controls. Adjustment for several putative confounders did not change the estimate (OR 3.02; 95% CI 1.26-7.22). Analysis of venous thrombotic risk influenced by PAI-1 genotype, showed no influence of PAI-1 4G/5G gene variant in comparison with 5G/5G genotype (OR 0.57 95% CI; 0.27-1.20).Decreased fibrinolytic activity increased, whereas PAI-1 4G/5G polymorphism did not influence venous thrombosis risk in this study.

9 CFR 325.1 - Transactions in commerce prohibited without official inspection legend or certificate when...

Code of Federal Regulations, 2010 CFR

2010-01-01

... requirements. 325.1 Section 325.1 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF... commerce, any product which is capable of use as human food unless the product and its container, if any... food product until a certificate, if required for such transportation by this part, is made and...

Effect of the G375C and G346E achondroplasia mutations on FGFR3 activation.

PubMed

He, Lijuan; Serrano, Christopher; Niphadkar, Nitish; Shobnam, Nadia; Hristova, Kalina

2012-01-01

Two mutations in FGFR3, G380R and G375C are known to cause achondroplasia, the most common form of human dwarfism. The G380R mutation accounts for 98% of the achondroplasia cases, and thus has been studied extensively. Here we study the effect of the G375C mutation on the phosphorylation and the cross-linking propensity of full-length FGFR3 in HEK 293 cells, and we compare the results to previously published results for the G380R mutant. We observe identical behavior of the two achondroplasia mutants in these experiments, a finding which supports a direct link between the severity of dwarfism phenotypes and the level and mechanism of FGFR3 over-activation. The mutations do not increase the cross-linking propensity of FGFR3, contrary to previous expectations that the achondroplasia mutations stabilize the FGFR3 dimers. Instead, the phosphorylation efficiency within un-liganded FGFR3 dimers is increased, and this increase is likely the underlying cause for pathogenesis in achondroplasia. We further investigate the G346E mutation, which has been reported to cause achondroplasia in one case. We find that this mutation does not increase FGFR3 phosphorylation and decreases FGFR3 cross-linking propensity, a finding which raises questions whether this mutation is indeed a genetic cause for human dwarfism.

Effect of the G375C and G346E Achondroplasia Mutations on FGFR3 Activation

PubMed Central

He, Lijuan; Serrano, Christopher; Niphadkar, Nitish; Shobnam, Nadia; Hristova, Kalina

2012-01-01

Two mutations in FGFR3, G380R and G375C are known to cause achondroplasia, the most common form of human dwarfism. The G380R mutation accounts for 98% of the achondroplasia cases, and thus has been studied extensively. Here we study the effect of the G375C mutation on the phosphorylation and the cross-linking propensity of full-length FGFR3 in HEK 293 cells, and we compare the results to previously published results for the G380R mutant. We observe identical behavior of the two achondroplasia mutants in these experiments, a finding which supports a direct link between the severity of dwarfism phenotypes and the level and mechanism of FGFR3 over-activation. The mutations do not increase the cross-linking propensity of FGFR3, contrary to previous expectations that the achondroplasia mutations stabilize the FGFR3 dimers. Instead, the phosphorylation efficiency within un-liganded FGFR3 dimers is increased, and this increase is likely the underlying cause for pathogenesis in achondroplasia. We further investigate the G346E mutation, which has been reported to cause achondroplasia in one case. We find that this mutation does not increase FGFR3 phosphorylation and decreases FGFR3 cross-linking propensity, a finding which raises questions whether this mutation is indeed a genetic cause for human dwarfism. PMID:22529939

35. Perimeter acquisition radar building room #325, showing hard disc ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. Perimeter acquisition radar building room #325, showing hard disc drive - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

Antitumor activity of a folate receptor-targeted immunoglobulin G-doxorubicin conjugate

PubMed Central

Yang, Tan; Xu, Ling; Li, Bin; Li, Weijie; Ma, Xiang; Fan, Lingling; Lee, Robert J; Xu, Chuanrui; Xiang, Guangya

2017-01-01

Development of antibody-drug conjugates (ADCs) is a promising therapeutic strategy for cancer therapy. In this study, folate was conjugated via a polyethyleneglycol (PEG) linker to immunoglobulin G (IgG), which was linked to doxorubicin (DOX), to form a novel ADC folate-PEG-IgG-DOX (FA-PEG-IgG-DOX). The FA-PEG-IgG-DOX showed high targeting efficiency in HeLa and KB cells and significantly improved the uptake and retention of DOX compared with IgG-DOX about 10-fold. Subsequently, FA-PEG-IgG-DOX was shown to have at least 8 times higher antitumor activity than IgG-DOX both in HeLa and KB cells and also induced more apoptosis in those cells than IgG-DOX. Moreover, FA-PEG-IgG-DOX had a 2 times longer circulating time than FA-IgG-DOX, but did not increase the DOX distribution in mouse hearts. Importantly, FA-PEG-IgG-DOX treatment significantly inhibited tumor growth in xenograft mice. Together, our results indicate that FA-PEG-IgG is an effective ADC carrier for delivery of chemotherapeutic agents and that conjugating tumor targeting ligands to antibodies is a promising strategy for producing ADC drugs. PMID:28408821

14 CFR 152.325 - Financial status report: Airport planning.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Financial status report: Airport planning... TRANSPORTATION (CONTINUED) AIRPORTS AIRPORT AID PROGRAM Accounting and Reporting Requirements § 152.325 Financial status report: Airport planning. Each sponsor of a project for airport master planning and each planning...

14 CFR 152.325 - Financial status report: Airport planning.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Financial status report: Airport planning... TRANSPORTATION (CONTINUED) AIRPORTS AIRPORT AID PROGRAM Accounting and Reporting Requirements § 152.325 Financial status report: Airport planning. Each sponsor of a project for airport master planning and each planning...

14 CFR 152.325 - Financial status report: Airport planning.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Financial status report: Airport planning... TRANSPORTATION (CONTINUED) AIRPORTS AIRPORT AID PROGRAM Accounting and Reporting Requirements § 152.325 Financial status report: Airport planning. Each sponsor of a project for airport master planning and each planning...

14 CFR 152.325 - Financial status report: Airport planning.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Financial status report: Airport planning... TRANSPORTATION (CONTINUED) AIRPORTS AIRPORT AID PROGRAM Accounting and Reporting Requirements § 152.325 Financial status report: Airport planning. Each sponsor of a project for airport master planning and each planning...

UV-induced changes of active components and antioxidant activity in postharvest pigeon pea [Cajanus cajan (L.) Millsp.] leaves.

PubMed

Wei, Zuo-Fu; Luo, Meng; Zhao, Chun-Jian; Li, Chun-Ying; Gu, Cheng-Bo; Wang, Wei; Zu, Yuan-Gang; Efferth, Thomas; Fu, Yu-Jie

2013-02-13

In this study, the effect of UV irradiation (UV-A, UV-B, and UV-C) on phytochemicals, total phenolics, and antioxidant activity of postharvest pigeon pea leaves was evaluated. The response of pigeon pea leaves to UV irradiation was phytochemical specific. UV-B and UV-C induced higher levels of phytochemicals, total phenolics, and antioxidant activity in pigeon pea leaves compared with UV-A. Furthermore, UV-B irradiation proved to possess a long-lasting effect on the levels of phenolics and antioxidant activity. After adapting for 48 h at 4 °C following 4 h UV-B irradiation, total phenolics and antioxidant activity were approximately 1.5-fold and 2.2-fold increased from 39.4 mg GAE/g DM and 15.0 μmol GAE/g DM to 59.1 mg GAE/g DM and 32.5 μmol GAE/g DM, respectively. These results indicate that UV irradiation of pigeon pea leaves can be beneficial in terms of increasing active components and antioxidant activity.

Phase-I investigation of high-efficiency power amplifiers for 325 and 650 MHz

DOE Office of Scientific and Technical Information (OSTI.GOV)

Raab, Frederick

2018-01-27

This Phase-I SBIR grant investigated techniques for high-efficiency power amplification for DoE particle accelerators such as Project X that operate at 325 and 650 MHz. The recommended system achieves high efficiency, high reliability, and hot-swap capability by integrating class-F power amplifiers, class-S modulators, power combiners, and a digital signal processor. Experimental evaluations demonstrate the production of 120 W per transistor with overall efficiencies from 86 percent at 325 MHz and 80 percent at 650 MHz.

Differential activation of G-proteins by mu-opioid receptor agonists.

PubMed

Saidak, Zuzana; Blake-Palmer, Katherine; Hay, Debbie L; Northup, John K; Glass, Michelle

2006-03-01

We investigated the ability of the activated mu-opioid receptor (MOR) to differentiate between myristoylated G(alphai1) and G(alphaoA) type G(alpha) proteins, and the maximal activity of a range of synthetic and endogenous agonists to activate each G(alpha) protein. Membranes from HEK293 cells stably expressing transfected MOR were chaotrope extracted to denature endogenous G-proteins and reconstituted with specific purified G-proteins. The G(alpha) subunits were generated in bacteria and were demonstrated to be recognised equivalently to bovine brain purified G(alpha) protein by CB(1) cannabinoid receptors. The ability of agonists to catalyse the MOR-dependent GDP/[(35)S]GTP(gamma)S exchange was then compared for G(alphai1) and G(alphaoA). Activation of MOR by DAMGO produced a high-affinity saturable interaction for G(alphaoA) (K(m)=20+/-1 nM) but a low-affinity interaction with G(alphai1) (K(m)=116+/-12 nM). DAMGO, met-enkephalin and leucine-enkephalin displayed maximal G(alpha) activation among the agonists evaluated. Endomorphins 1 and 2, methadone and beta-endorphin activated both G(alpha) to more than 75% of the maximal response, whereas fentanyl partially activated both G-proteins. Buprenorphine and morphine demonstrated a statistically significant difference between the maximal activities between G(alphai1) and G(alphaoA). Interestingly, DAMGO, morphine, endomorphins 1 and 2, displayed significant differences in the potencies for the activation of the two G(alpha). Differences in maximal activity and potency, for G(alphai1) versus G(alphaoA), are both indicative of agonist selective activation of G-proteins in response to MOR activation. These findings may provide a starting point for the design of drugs that demonstrate greater selectivity between these two G-proteins and therefore produce a more limited range of effects.

40 CFR 89.325 - Engine intake air temperature measurement.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 21 2013-07-01 2013-07-01 false Engine intake air temperature... Test Equipment Provisions § 89.325 Engine intake air temperature measurement. (a) Engine intake air temperature measurement must be made within 122 cm of the engine. The measurement location must be made either...

40 CFR 89.325 - Engine intake air temperature measurement.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Engine intake air temperature... Test Equipment Provisions § 89.325 Engine intake air temperature measurement. (a) Engine intake air temperature measurement must be made within 122 cm of the engine. The measurement location must be made either...

40 CFR 89.325 - Engine intake air temperature measurement.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Engine intake air temperature... Test Equipment Provisions § 89.325 Engine intake air temperature measurement. (a) Engine intake air temperature measurement must be made within 122 cm of the engine. The measurement location must be made either...

40 CFR 89.325 - Engine intake air temperature measurement.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 21 2012-07-01 2012-07-01 false Engine intake air temperature... Test Equipment Provisions § 89.325 Engine intake air temperature measurement. (a) Engine intake air temperature measurement must be made within 122 cm of the engine. The measurement location must be made either...

47 CFR 1.6002 - Form and content.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 1 2010-10-01 2010-10-01 false Form and content. 1.6002 Section 1.6002 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL PRACTICE AND PROCEDURE Implementation of Section 325(e) of the Communications Act: Procedures Governing Complaints Filed by Television Broadcast Stations Against Satellite Carriers for...

Adenylyl cyclase G is activated by an intramolecular osmosensor.

PubMed

Saran, Shweta; Schaap, Pauline

2004-03-01

Adenylyl cyclase G (ACG) is activated by high osmolality and mediates inhibition of spore germination by this stress factor. The catalytic domains of all eukaryote cyclases are active as dimers and dimerization often mediates activation. To investigate the role of dimerization in ACG activation, we coexpressed ACG with an ACG construct that lacked the catalytic domain (ACGDeltacat) and was driven by a UV-inducible promoter. After UV induction of ACGDeltacat, cAMP production by ACG was strongly inhibited, but osmostimulation was not reduced. Size fractionation of native ACG showed that dimers were formed between ACG molecules and between ACG and ACGDeltacat. However, high osmolality did not alter the dimer/monomer ratio. This indicates that ACG activity requires dimerization via a region outside the catalytic domain but that dimer formation does not mediate activation by high osmolality. To establish whether ACG required auxiliary sensors for osmostimulation, we expressed ACG cDNA in a yeast adenylyl cyclase null mutant. In yeast, cAMP production by ACG was similarly activated by high osmolality as in Dictyostelium. This strongly suggests that the ACG osmosensor is intramolecular, which would define ACG as the first characterized primary osmosensor in eukaryotes.

Influence of aggregate size on the binding and activation of the first component of human complement by soluble IgG aggregates.

PubMed Central

Doekes, G; Vanes, L A; Daha, M R

1982-01-01

The interaction between small aggregates of human IgG and the first component of human complement was studied. Stabilized soluble IgG aggregates of restricted size were prepared by heat aggregation of human IgG, followed by sucrose-density ultracentrifugation. Human C1 was isolated in its precursor form by euglobulin precipitation, followed by gel filtration and immunoadsorption. A C1 preparation was obtained of which more than 90% was still in its unactivated form. Soluble aggregates containing 20, 10 or 5 molecules IgG, and monomeric IgG were tested for their ability to bind and to activate C1. The binding of C1 was determined by C1 consumption, whereas the activation of C1 was measured as the increased ability of the C1 preparation to consume purified human C4 after the incubation with the aggregates. The three aggregates tested and monomeric IgG were all able to bind and to activate C1, but the efficiency of both processes markedly increased with increasing aggregate-size. Furthermore, it was found that all four preparations activated an appreciable amount of C1 at concentrations that did not result in any detectable C1 fixation. These results confirm earlier suggestion that C1 can be activated during a short, transient binding to small aggregates or immune complexes that have a low avidity for C1, after which the activated form, C1, is released into the medium. PMID:7068172

Quadruplexes in 'Dicty': crystal structure of a four-quartet G-quadruplex formed by G-rich motif found in the Dictyostelium discoideum genome.

PubMed

Guédin, Aurore; Lin, Linda Yingqi; Armane, Samir; Lacroix, Laurent; Mergny, Jean-Louis; Thore, Stéphane; Yatsunyk, Liliya A

2018-06-01

Guanine-rich DNA has the potential to fold into non-canonical G-quadruplex (G4) structures. Analysis of the genome of the social amoeba Dictyostelium discoideum indicates a low number of sequences with G4-forming potential (249-1055). Therefore, D. discoideum is a perfect model organism to investigate the relationship between the presence of G4s and their biological functions. As a first step in this investigation, we crystallized the dGGGGGAGGGGTACAGGGGTACAGGGG sequence from the putative promoter region of two divergent genes in D. discoideum. According to the crystal structure, this sequence folds into a four-quartet intramolecular antiparallel G4 with two lateral and one diagonal loops. The G-quadruplex core is further stabilized by a G-C Watson-Crick base pair and a A-T-A triad and displays high thermal stability (Tm > 90°C at 100 mM KCl). Biophysical characterization of the native sequence and loop mutants suggests that the DNA adopts the same structure in solution and in crystalline form, and that loop interactions are important for the G4 stability but not for its folding. Four-tetrad G4 structures are sparse. Thus, our work advances understanding of the structural diversity of G-quadruplexes and yields coordinates for in silico drug screening programs and G4 predictive tools.

5 CFR 9701.325 - Treatment of employees whose rate of basic pay falls below the minimum rate of their band.

Code of Federal Regulations, 2014 CFR

2014-01-01

... basic pay falls below the minimum rate of their band. 9701.325 Section 9701.325 Administrative Personnel... Administration Setting and Adjusting Rate Ranges § 9701.325 Treatment of employees whose rate of basic pay falls... under § 9701.323 because of an unacceptable rating of record and whose rate of basic pay falls below the...

5 CFR 9701.325 - Treatment of employees whose rate of basic pay falls below the minimum rate of their band.

Code of Federal Regulations, 2011 CFR

2011-01-01

... basic pay falls below the minimum rate of their band. 9701.325 Section 9701.325 Administrative Personnel... Administration Setting and Adjusting Rate Ranges § 9701.325 Treatment of employees whose rate of basic pay falls... under § 9701.323 because of an unacceptable rating of record and whose rate of basic pay falls below the...

5 CFR 9701.325 - Treatment of employees whose rate of basic pay falls below the minimum rate of their band.

Code of Federal Regulations, 2012 CFR

2012-01-01

... basic pay falls below the minimum rate of their band. 9701.325 Section 9701.325 Administrative Personnel... Administration Setting and Adjusting Rate Ranges § 9701.325 Treatment of employees whose rate of basic pay falls... under § 9701.323 because of an unacceptable rating of record and whose rate of basic pay falls below the...

5 CFR 9701.325 - Treatment of employees whose rate of basic pay falls below the minimum rate of their band.

Code of Federal Regulations, 2013 CFR

2013-01-01

... basic pay falls below the minimum rate of their band. 9701.325 Section 9701.325 Administrative Personnel... Administration Setting and Adjusting Rate Ranges § 9701.325 Treatment of employees whose rate of basic pay falls... under § 9701.323 because of an unacceptable rating of record and whose rate of basic pay falls below the...

Short loop length and high thermal stability determine genomic instability induced by G-quadruplex-forming minisatellites

PubMed Central

Piazza, Aurèle; Adrian, Michael; Samazan, Frédéric; Heddi, Brahim; Hamon, Florian; Serero, Alexandre; Lopes, Judith; Teulade-Fichou, Marie-Paule; Phan, Anh Tuân; Nicolas, Alain

2015-01-01

G-quadruplexes (G4) are polymorphic four-stranded structures formed by certain G-rich nucleic acids, with various biological roles. However, structural features dictating their formation and/or functionin vivo are unknown. InS. cerevisiae, the pathological persistency of G4 within the CEB1 minisatellite induces its rearrangement during leading-strand replication. We now show that several other G4-forming sequences remain stable. Extensive mutagenesis of the CEB25 minisatellite motif reveals that only variants with very short (≤ 4 nt) G4 loops preferentially containing pyrimidine bases trigger genomic instability. Parallel biophysical analyses demonstrate that shortening loop length does not change the monomorphic G4 structure of CEB25 variants but drastically increases its thermal stability, in correlation with thein vivo instability. Finally, bioinformatics analyses reveal that the threat for genomic stability posed by G4 bearing short pyrimidine loops is conserved inC. elegans and humans. This work provides a framework explanation for the heterogeneous instability behavior of G4-forming sequencesin vivo, highlights the importance of structure thermal stability, and questions the prevailing assumption that G4 structures with short or longer loops are as likely to formin vivo. PMID:25956747

12 CFR 325.201 - Authority, purpose, and reservation of authority.

Code of Federal Regulations, 2013 CFR

2013-01-01

... STATEMENTS OF GENERAL POLICY CAPITAL MAINTENANCE Annual Stress Test § 325.201 Authority, purpose, and... require each covered bank to conduct annual stress tests and establishes a definition of stress test, methodologies for conducting stress tests, and reporting and disclosure requirements. (c) Reservation of...

12 CFR 325.201 - Authority, purpose, and reservation of authority.

Code of Federal Regulations, 2014 CFR

2014-01-01

... STATEMENTS OF GENERAL POLICY CAPITAL MAINTENANCE Annual Stress Test § 325.201 Authority, purpose, and... require each covered bank to conduct annual stress tests and establishes a definition of stress test, methodologies for conducting stress tests, and reporting and disclosure requirements. (c) Reservation of...

20 CFR 325.2 - Procedure for registering for unemployment benefits.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Procedure for registering for unemployment... UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.2 Procedure for registering for unemployment benefits. (a) Registering as unemployed. To claim unemployment benefits for any day...

20 CFR 325.2 - Procedure for registering for unemployment benefits.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true Procedure for registering for unemployment... UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.2 Procedure for registering for unemployment benefits. (a) Registering as unemployed. To claim unemployment benefits for any day...

20 CFR 325.2 - Procedure for registering for unemployment benefits.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true Procedure for registering for unemployment... UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.2 Procedure for registering for unemployment benefits. (a) Registering as unemployed. To claim unemployment benefits for any day...

20 CFR 325.2 - Procedure for registering for unemployment benefits.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false Procedure for registering for unemployment... UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.2 Procedure for registering for unemployment benefits. (a) Registering as unemployed. To claim unemployment benefits for any day...

20 CFR 325.2 - Procedure for registering for unemployment benefits.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false Procedure for registering for unemployment... UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.2 Procedure for registering for unemployment benefits. (a) Registering as unemployed. To claim unemployment benefits for any day...

12 CFR Appendix G to Part 226 - Open-End Model Forms and Clauses

Code of Federal Regulations, 2011 CFR

2011-01-01

... RESERVE SYSTEM TRUTH IN LENDING (REGULATION Z) Pt. 226, App. G Appendix G to Part 226—Open-End Model Forms... charges]. We do not add in any new [purchases/advances/loans]. This gives us the daily balance. Then, we... “average daily balance” we take the beginning balance of your account each day, add any new [purchases...

A pore-forming protein implements VLR-activated complement cytotoxicity in lamprey.

PubMed

Wu, Fenfang; Feng, Bo; Ren, Yong; Wu, Di; Chen, Yue; Huang, Shengfeng; Chen, Shangwu; Xu, Anlong

2017-01-01

Lamprey is a basal vertebrate with a unique adaptive immune system, which uses variable lymphocyte receptors (VLRs) for antigen recognition. Our previous study has shown that lamprey possessed a distinctive complement pathway activated by VLR. In this study, we identified a natterin family member-lamprey pore-forming protein (LPFP) with a jacalin-like lectin domain and an aerolysin-like pore-forming domain. LPFP had a high affinity with mannan and could form oligomer in the presence of mannan. LPFP could deposit on the surface of target cells, form pore-like complex resembling a wheel with hub and spokes, and mediate powerful cytotoxicity on target cells. These pore-forming proteins along with VLRs and complement molecules were essential for the specific cytotoxicity against exogenous pathogens and tumor cells. This unique cytotoxicity implemented by LPFP might emerge before or in parallel with the IgG-based classical complement lytic pathway completed by polyC9.

Butyrylcholinesterase: K variant, plasma activity, molecular forms and rivastigmine treatment in Alzheimer's disease in a Southern Brazilian population.

PubMed

Bono, G F; Simão-Silva, D P; Batistela, M S; Josviak, N D; Dias, P F R; Nascimento, G A; Souza, R L R; Piovezan, M R; Souza, R K M; Furtado-Alle, L

2015-02-01

Alzheimer's disease (AD) is a neurodegenerative disorder in which there is a decline of cholinergic function. The symptomatic AD treatment involves the use of ChEIs (cholinesterase inhibitors) as rivastigimine, a dual inhibitor. The human butyrylcholinesterase (BChE) is an enzyme that has specific roles in cholinergic neurotransmission and it has been associated with AD. In the serum, BChE is found in four main molecular forms: G1 (monomer); G1-ALB (monomer linked to albumin); G2 (dimer); and G4 (tetramer). The interaction between the products of BCHE gene and CHE2 locus results in CHE2 C5+ and CHE2 C5- phenotypes. CHE2 C5+ phenotype and BChE-K are factors that influence on BChE activity. This work aimed to verify the proportions of BChE molecular forms, total and relative activity in 139 AD patients and 139 elderly controls, taking into account K variant, CHE2 locus, rivastigmine treatment and clinical dementia rating (CDR) of AD patients. Phenotypic frequencies of CHE2 C5+ and frequency of the carriers of the K allele were similar between groups. Total BChE activity in plasma was significantly lower in AD patients than in elderly controls. Furthermore, we found that reduction on plasma BChE activity is associated directly with AD progression in AD patients and that rivastigmine treatment has a stronger effect on BChE activity within the CDR2 group. The reduction in BChE activity did not occur proportionally in all molecular forms. Multiple regression analysis results confirmed that AD acts as the main factor in plasma BChE activity reduction and that severe stages are related with an even greater reduction. These findings suggest that the reduction of total plasma BChE and relative BChE molecular forms activity in AD patients is probably associated with a feedback mechanism and provides a future perspective of using this enzyme as a possible plasmatic secondary marker for AD. Copyright © 2014 Elsevier Ltd. All rights reserved.

Isoguanine quartets formed by d(T4isoG4T4): tetraplex identification and stability.

PubMed Central

Seela, F; Wei, C; Melenewski, A

1996-01-01

The self-aggregation of the oligonucleotide d(T4isoG4T4) (1) is investigated. Based on ion exchange HPLC experiments and CD spectroscopy, a tetrameric structure is identified. This structure was formed in the presence of sodium ions and shows almost the same chromatographic mobility on ion exchange HPLC as d(T4G4T4) (2). The ratio of aggregate versus monomer is temperature dependent and the tetraplex of [d(T4isoG4T4)]4 is more stable than that of [d(T4G4T4)]4. A mixture of d(T4isoG4T4) and d(T4G4T4) forms mixed tetraplexes containing strands of d(T4isoG4T4) and d(T4G4T4). PMID:9016664

20 CFR 411.325 - What reporting requirements are placed on an EN as a participant in the Ticket to Work program?

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false What reporting requirements are placed on an EN as a participant in the Ticket to Work program? 411.325 Section 411.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Employment Networks § 411.325 What...

20 CFR 411.325 - What reporting requirements are placed on an EN as a participant in the Ticket to Work program?

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false What reporting requirements are placed on an EN as a participant in the Ticket to Work program? 411.325 Section 411.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Employment Networks § 411.325 What...

20 CFR 411.325 - What reporting requirements are placed on an EN as a participant in the Ticket to Work program?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false What reporting requirements are placed on an EN as a participant in the Ticket to Work program? 411.325 Section 411.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Employment Networks § 411.325 What...

20 CFR 411.325 - What reporting requirements are placed on an EN as a participant in the Ticket to Work program?

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false What reporting requirements are placed on an EN as a participant in the Ticket to Work program? 411.325 Section 411.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Employment Networks § 411.325 What...

20 CFR 411.325 - What reporting requirements are placed on an EN as a participant in the Ticket to Work program?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false What reporting requirements are placed on an EN as a participant in the Ticket to Work program? 411.325 Section 411.325 Employees' Benefits SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Employment Networks § 411.325 What...

29 CFR Appendix G to Part 825 - Certification of Qualifying Exigency for Military Family Leave (Form WH-384)

Code of Federal Regulations, 2010 CFR

2010-07-01

... Leave (Form WH-384) G Appendix G to Part 825 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS THE FAMILY AND MEDICAL LEAVE ACT OF 1993 Pt. 825, App. G Appendix G to Part 825—Certification of Qualifying Exigency for Military Family Leave (Form WH-384...

29 CFR Appendix G to Part 825 - Certification of Qualifying Exigency for Military Family Leave (Form WH-384)

Code of Federal Regulations, 2012 CFR

2012-07-01

... Leave (Form WH-384) G Appendix G to Part 825 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS THE FAMILY AND MEDICAL LEAVE ACT OF 1993 Pt. 825, App. G Appendix G to Part 825—Certification of Qualifying Exigency for Military Family Leave (Form WH-384...

29 CFR Appendix G to Part 825 - Certification of Qualifying Exigency for Military Family Leave (Form WH-384)

Code of Federal Regulations, 2011 CFR

2011-07-01

... Leave (Form WH-384) G Appendix G to Part 825 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS THE FAMILY AND MEDICAL LEAVE ACT OF 1993 Pt. 825, App. G Appendix G to Part 825—Certification of Qualifying Exigency for Military Family Leave (Form WH-384...

46 CFR 178.325 - Intact stability requirements-monohull sailing vessels.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Intact stability requirements-monohull sailing vessels....325 Intact stability requirements—monohull sailing vessels. (a) As permitted by § 178.310(c) of this part, a monohull sailing vessel may demonstrate compliance with paragraphs (b) or (c) of this section...

Vibrational resonance, allostery, and activation in rhodopsin-like G protein-coupled receptors

PubMed Central

Woods, Kristina N.; Pfeffer, Jürgen; Dutta, Arpana; Klein-Seetharaman, Judith

2016-01-01

G protein-coupled receptors are a large family of membrane proteins activated by a variety of structurally diverse ligands making them highly adaptable signaling molecules. Despite recent advances in the structural biology of this protein family, the mechanism by which ligands induce allosteric changes in protein structure and dynamics for its signaling function remains a mystery. Here, we propose the use of terahertz spectroscopy combined with molecular dynamics simulation and protein evolutionary network modeling to address the mechanism of activation by directly probing the concerted fluctuations of retinal ligand and transmembrane helices in rhodopsin. This approach allows us to examine the role of conformational heterogeneity in the selection and stabilization of specific signaling pathways in the photo-activation of the receptor. We demonstrate that ligand-induced shifts in the conformational equilibrium prompt vibrational resonances in the protein structure that link the dynamics of conserved interactions with fluctuations of the active-state ligand. The connection of vibrational modes creates an allosteric association of coupled fluctuations that forms a coherent signaling pathway from the receptor ligand-binding pocket to the G-protein activation region. Our evolutionary analysis of rhodopsin-like GPCRs suggest that specific allosteric sites play a pivotal role in activating structural fluctuations that allosterically modulate functional signals. PMID:27849063

22 CFR 1006.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-04-01

... disallow costs, annul or terminate the transaction, issue a stop work order, debar or suspend you, or take... person in a covered transaction? 1006.325 Section 1006.325 Foreign Relations INTER-AMERICAN FOUNDATION GOVERNMENTWIDE DEBARMENT AND SUSPENSION (NONPROCUREMENT) Responsibilities of Participants Regarding Transactions...

29 CFR 98.325 - What happens if I do business with an excluded person in a covered transaction?

Code of Federal Regulations, 2010 CFR

2010-07-01

... transaction? If as a participant you knowingly do business with an excluded person, we may disallow costs... covered transaction? 98.325 Section 98.325 Labor Office of the Secretary of Labor GOVERNMENTWIDE DEBARMENT AND SUSPENSION (NONPROCUREMENT) Responsibilities of Participants Regarding Transactions Doing Business...

Mutated form (G52E) of inactive diphtheria toxin CRM197: molecular simulations clearly display effect of the mutation to NAD binding.

PubMed

Salmas, Ramin Ekhteiari; Mestanoglu, Mert; Unlu, Ayhan; Yurtsever, Mine; Durdagi, Serdar

2016-11-01

Mutated form (G52E) of diphtheria toxin (DT) CRM197 is an inactive and nontoxic enzyme. Here, we provided a molecular insight using comparative molecular dynamics (MD) simulations to clarify the influence of a single point mutation on overall protein and active-site loop. Post-processing MD analysis (i.e. stability, principal component analysis, hydrogen-bond occupancy, etc.) is carried out on both wild and mutated targets to investigate and to better understand the mechanistic differences of structural and dynamical properties on an atomic scale especially at nicotinamide adenine dinucleotide (NAD) binding site when a single mutation (G52E) happens at the DT. In addition, a docking simulation is performed for wild and mutated forms. The docking scoring analysis and docking poses results revealed that mutant form is not able to properly accommodate the NAD molecule.

Isolation and structure-function characterization of a signaling-active rhodopsin-G protein complex.

PubMed

Gao, Yang; Westfield, Gerwin; Erickson, Jon W; Cerione, Richard A; Skiniotis, Georgios; Ramachandran, Sekar

2017-08-25

The visual photo-transduction cascade is a prototypical G protein-coupled receptor (GPCR) signaling system, in which light-activated rhodopsin (Rho*) is the GPCR catalyzing the exchange of GDP for GTP on the heterotrimeric G protein transducin (G T ). This results in the dissociation of G T into its component α T -GTP and β 1 γ 1 subunit complex. Structural information for the Rho*-G T complex will be essential for understanding the molecular mechanism of visual photo-transduction. Moreover, it will shed light on how GPCRs selectively couple to and activate their G protein signaling partners. Here, we report on the preparation of a stable detergent-solubilized complex between Rho* and a heterotrimer (G T *) comprising a Gα T /Gα i1 chimera (α T *) and β 1 γ 1 The complex was formed on native rod outer segment membranes upon light activation, solubilized in lauryl maltose neopentyl glycol, and purified with a combination of affinity and size-exclusion chromatography. We found that the complex is fully functional and that the stoichiometry of Rho* to Gα T * is 1:1. The molecular weight of the complex was calculated from small-angle X-ray scattering data and was in good agreement with a model consisting of one Rho* and one G T *. The complex was visualized by negative-stain electron microscopy, which revealed an architecture similar to that of the β 2 -adrenergic receptor-G S complex, including a flexible α T * helical domain. The stability and high yield of the purified complex should allow for further efforts toward obtaining a high-resolution structure of this important signaling complex. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Formaldehyde activation of mitoxantrone yields CpG and CpA specific DNA adducts

PubMed Central

Parker, Belinda S.; Cutts, Suzanne M.; Cullinane, Carleen; Phillips, Don R.

2000-01-01

Recently we have found that mitoxantrone, like Adriamycin, can be activated by formaldehyde and subsequently form adducts which stabilise double-stranded DNA in vitro. This activation by formaldehyde may be biologically relevant since formaldehyde levels are elevated in those tumours in which mitoxantrone is most cytotoxic. In vitro transcription analysis revealed that these adducts block the progression of RNA polymerase during transcription and cause truncated RNA transcripts. There was an absolute requirement for both mitoxantrone and formaldehyde in transcriptional blockage formation and the activated complex was found to exhibit site specificity, with blockage occurring prior to CpG and CpA sites in the DNA (non-template strand). The stability of the adduct at 37°C was site dependent. The half-lives ranged from 45 min to ~5 h and this was dependent on both the central 2 bp blockage site as well as flanking sequences. The CpG specificity of mitoxantrone adduct sites was also confirmed independently by a λ exonuclease digestion assay. PMID:10648792

9 CFR 325.18 - Diverting of shipments, breaking of seals, and reloading by carrier in emergency; reporting to...

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Diverting of shipments, breaking of seals, and reloading by carrier in emergency; reporting to Regional Director. 325.18 Section 325.18 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY...

9 CFR 325.17 - Loading or unloading products in sealed railroad cars, trucks, etc., en route prohibited; exception.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Loading or unloading products in sealed railroad cars, trucks, etc., en route prohibited; exception. 325.17 Section 325.17 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION AND...

9 CFR 325.17 - Loading or unloading products in sealed railroad cars, trucks, etc., en route prohibited; exception.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Loading or unloading products in sealed railroad cars, trucks, etc., en route prohibited; exception. 325.17 Section 325.17 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION AND...

49 CFR 325.73 - Microphone distance correction factors. 1

Code of Federal Regulations, 2013 CFR

2013-10-01

... the observed sound level reading is— 31 feet (9.5 m) or more but less than 35 feet (10.7 m) −4 35 feet... more but less than 83 feet (25.3 m) +2 [40 FR 42437, Sept. 12, 1975, as amended at 54 FR 50385, Dec. 6... 49 Transportation 5 2013-10-01 2013-10-01 false Microphone distance correction factors. 1 325.73...

49 CFR 325.73 - Microphone distance correction factors. 1

Code of Federal Regulations, 2012 CFR

2012-10-01

... the observed sound level reading is— 31 feet (9.5 m) or more but less than 35 feet (10.7 m) −4 35 feet... more but less than 83 feet (25.3 m) +2 [40 FR 42437, Sept. 12, 1975, as amended at 54 FR 50385, Dec. 6... 49 Transportation 5 2012-10-01 2012-10-01 false Microphone distance correction factors. 1 325.73...

49 CFR 325.73 - Microphone distance correction factors. 1

Code of Federal Regulations, 2014 CFR

2014-10-01

... the observed sound level reading is— 31 feet (9.5 m) or more but less than 35 feet (10.7 m) −4 35 feet... more but less than 83 feet (25.3 m) +2 [40 FR 42437, Sept. 12, 1975, as amended at 54 FR 50385, Dec. 6... 49 Transportation 5 2014-10-01 2014-10-01 false Microphone distance correction factors. 1 325.73...

30 CFR 251.4 - Types of G&G activities that require permits or Notices.

Code of Federal Regulations, 2011 CFR

2011-07-01

... separate permit for each. (b) Scientific research. You may only conduct G&G scientific research related to... proprietary use or sale. (2) Notice. Any other G&G scientific research that you conduct related to oil, gas.... You must obtain a permit if the research activities you propose to conduct involve: (i) Using solid or...

30 CFR 251.4 - Types of G&G activities that require permits or Notices.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) Scientific research. You may only conduct G&G scientific research related to oil, gas, and sulphur in the OCS...) Notice. Any other G&G scientific research that you conduct related to oil, gas, and sulphur in the OCS... research activities you propose to conduct involve: (i) Using solid or liquid explosives; (ii) Drilling a...

40 CFR 1033.325 - Maintenance of records; submittal of information.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Production Line Testing and Audit Programs § 1033.325 Maintenance of records; submittal of information. (a... production line test or audit including: (i) The date, time, and location of each test or audit. (ii) The method by which the Green Engine Factor was calculated or the number of hours of service accumulated on...

15 CFR 325.6 - Publishing notices in the Federal Register.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Publishing notices in the Federal... CERTIFICATES OF REVIEW § 325.6 Publishing notices in the Federal Register. (a) Within ten days after an... conduct for which certification is sought. If the Secretary does not intend to publish the summary...

The Huntington disease locus is most likely within 325 kilobases of the chromosome 4p telomere

DOE Office of Scientific and Technical Information (OSTI.GOV)

Doggett, N.A.; Cheng, J.F.; Smith, C.L.

1989-12-01

The genetic defect responsible for Huntington disease was originally localized near the tip of the short arm of chromosome 4 by genetic linkage to the locus D4S10. Several markers closer to Huntington disease have since been isolated, but these all appear to be proximal to the defect. A physical map that extends from the most distal of these loci, D4S90, to the telomere of chromosome 4 was constructed. This map identifies at least two CpG islands as markers for Huntington disease candidate genes and places the most likely location of the Huntington disease defect remarkably close (within 325 kilobases) tomore » the telomere.« less

Lack of effect of the alpha2C-adrenoceptor Del322-325 polymorphism on inhibition of cyclic AMP production in HEK293 cells.

PubMed

Montgomery, M D; Bylund, D B

2010-02-01

The alpha(2C)-adrenoceptor has multiple functions, including inhibiting release of noradrenaline from presynaptic nerve terminals. A human alpha(2C) polymorphism, Del322-325, a potential risk factor for heart failure, has been reported to exhibit reduced signalling in CHO cells. To further understand the role of the Del322-325 polymorphism on receptor signalling, we attempted to replicate and further study the reduced signalling in HEK293 cells. Human alpha(2C) wild-type (WT) and Del322-325 adrenoceptors were stably transfected into HEK293 cells. Radioligand binding was performed to determine affinities for both receptors. In intact cells, inhibition of forskolin-stimulated cyclic AMP production by WT and Del322-325 clones with a range of receptor densities (200-2320 fmol.mg(-1) protein) was measured following agonist treatment. Noradrenaline, brimonidine and clonidine exhibited similar binding affinities for WT and Del322-325. Brimonidine and clonidine also had similar efficacies and potencies for both receptors for the inhibition of cyclic AMP production at all receptor densities tested. A linear regression analysis comparing efficacy and potency with receptor expression levels showed no differences in slopes between WT and Del322-325. The alpha(2C) WT and Del322-325 adrenoceptors exhibited similar binding properties. Additionally, inhibition of cyclic AMP production by Del322-325 was similar to that of WT over a range of receptor densities. Therefore, in intact HEK293 cells, the alpha(2C)-Del322-325 polymorphism does not exhibit reduced signalling to adenylyl cyclase and may not represent a clinically important phenotype.

50 CFR 32.5 - What are the requirements for sportfishing on areas of the National Wildlife Refuge System?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 8 2011-10-01 2011-10-01 false What are the requirements for sportfishing on areas of the National Wildlife Refuge System? 32.5 Section 32.5 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) THE NATIONAL WILDLIFE REFUGE...

50 CFR 32.5 - What are the requirements for sportfishing on areas of the National Wildlife Refuge System?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 9 2012-10-01 2012-10-01 false What are the requirements for sportfishing on areas of the National Wildlife Refuge System? 32.5 Section 32.5 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) THE NATIONAL WILDLIFE REFUGE...

50 CFR 32.5 - What are the requirements for sportfishing on areas of the National Wildlife Refuge System?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 9 2013-10-01 2013-10-01 false What are the requirements for sportfishing on areas of the National Wildlife Refuge System? 32.5 Section 32.5 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) THE NATIONAL WILDLIFE REFUGE...

50 CFR 32.5 - What are the requirements for sportfishing on areas of the National Wildlife Refuge System?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false What are the requirements for sportfishing on areas of the National Wildlife Refuge System? 32.5 Section 32.5 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) THE NATIONAL WILDLIFE REFUGE...

50 CFR 32.5 - What are the requirements for sportfishing on areas of the National Wildlife Refuge System?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 9 2014-10-01 2014-10-01 false What are the requirements for sportfishing on areas of the National Wildlife Refuge System? 32.5 Section 32.5 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) THE NATIONAL WILDLIFE REFUGE...

The yeast Pif1 helicase prevents genomic instability caused by G-quadruplex-forming CEB1 sequences in vivo.

PubMed

Ribeyre, Cyril; Lopes, Judith; Boulé, Jean-Baptiste; Piazza, Aurèle; Guédin, Aurore; Zakian, Virginia A; Mergny, Jean-Louis; Nicolas, Alain

2009-05-01

In budding yeast, the Pif1 DNA helicase is involved in the maintenance of both nuclear and mitochondrial genomes, but its role in these processes is still poorly understood. Here, we provide evidence for a new Pif1 function by demonstrating that its absence promotes genetic instability of alleles of the G-rich human minisatellite CEB1 inserted in the Saccharomyces cerevisiae genome, but not of other tandem repeats. Inactivation of other DNA helicases, including Sgs1, had no effect on CEB1 stability. In vitro, we show that CEB1 repeats formed stable G-quadruplex (G4) secondary structures and the Pif1 protein unwinds these structures more efficiently than regular B-DNA. Finally, synthetic CEB1 arrays in which we mutated the potential G4-forming sequences were no longer destabilized in pif1Delta cells. Hence, we conclude that CEB1 instability in pif1Delta cells depends on the potential to form G-quadruplex structures, suggesting that Pif1 could play a role in the metabolism of G4-forming sequences.

Activation of Escherichia coli antiterminator BglG requires its phosphorylation

PubMed Central

Rothe, Fabian M.; Bahr, Thomas; Stülke, Jörg; Rak, Bodo; Görke, Boris

2012-01-01

Transcriptional antiterminator proteins of the BglG family control the expression of enzyme II (EII) carbohydrate transporters of the bacterial phosphotransferase system (PTS). In the PTS, phosphoryl groups are transferred from phosphoenolpyruvate (PEP) via the phosphotransferases enzyme I (EI) and HPr to the EIIs, which phosphorylate their substrates during transport. Activity of the antiterminators is negatively controlled by reversible phosphorylation catalyzed by the cognate EIIs in response to substrate availability and positively controlled by the PTS. For the Escherichia coli BglG antiterminator, two different mechanisms for activation by the PTS were proposed. According to the first model, BglG is activated by HPr-catalyzed phosphorylation at a site distinct from the EII-dependent phosphorylation site. According to the second model, BglG is not activated by phosphorylation, but solely through interaction with EI and HPr, which are localized at the cell pole. Subsequently BglG is released from the cell pole to the cytoplasm as an active dimer. Here we addressed this discrepancy and found that activation of BglG requires phosphorylatable HPr or the HPr homolog FruB in vivo. Further, we uniquely demonstrate that purified BglG protein becomes phosphorylated by FruB as well as by HPr in vitro. Histidine residue 208 in BglG is essential for this phosphorylation. These data suggest that BglG is in fact activated by phosphorylation and that there is no principal difference between the PTS-exerted mechanisms controlling the activities of BglG family proteins in Gram-positive and Gram-negative bacteria. PMID:22984181

Credit BG. View looks north northwest (325°) towards Administration Building ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Credit BG. View looks north northwest (325°) towards Administration Building across aircraft apron east of Building 4505. Building 4507 (Storage Building) appears at extreme right of view - Edwards Air Force Base, North Base, Administration Building, Northeast of A Street, Boron, Kern County, CA

20 CFR 325.3 - Application for unemployment benefits and employment service.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Application for unemployment benefits and... RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.3 Application for unemployment benefits and employment service. (a) Requirement. An unemployed employee who wishes to claim...

20 CFR 325.3 - Application for unemployment benefits and employment service.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false Application for unemployment benefits and... RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.3 Application for unemployment benefits and employment service. (a) Requirement. An unemployed employee who wishes to claim...

20 CFR 325.3 - Application for unemployment benefits and employment service.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true Application for unemployment benefits and... RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.3 Application for unemployment benefits and employment service. (a) Requirement. An unemployed employee who wishes to claim...

20 CFR 325.3 - Application for unemployment benefits and employment service.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false Application for unemployment benefits and... RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.3 Application for unemployment benefits and employment service. (a) Requirement. An unemployed employee who wishes to claim...

20 CFR 325.3 - Application for unemployment benefits and employment service.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true Application for unemployment benefits and... RAILROAD UNEMPLOYMENT INSURANCE ACT REGISTRATION FOR RAILROAD UNEMPLOYMENT BENEFITS § 325.3 Application for unemployment benefits and employment service. (a) Requirement. An unemployed employee who wishes to claim...

Early-onset epileptic encephalopathy caused by a reduced sensitivity of Kv7.2 potassium channels to phosphatidylinositol 4,5-bisphosphate

PubMed Central

Soldovieri, Maria Virginia; Ambrosino, Paolo; Mosca, Ilaria; De Maria, Michela; Moretto, Edoardo; Miceli, Francesco; Alaimo, Alessandro; Iraci, Nunzio; Manocchio, Laura; Medoro, Alessandro; Passafaro, Maria; Taglialatela, Maurizio

2016-01-01

Kv7.2 and Kv7.3 subunits underlie the M-current, a neuronal K+ current characterized by an absolute functional requirement for phosphatidylinositol 4,5-bisphosphate (PIP2). Kv7.2 gene mutations cause early-onset neonatal seizures with heterogeneous clinical outcomes, ranging from self-limiting benign familial neonatal seizures to severe early-onset epileptic encephalopathy (Kv7.2-EE). In this study, the biochemical and functional consequences prompted by a recurrent variant (R325G) found independently in four individuals with severe forms of neonatal-onset EE have been investigated. Upon heterologous expression, homomeric Kv7.2 R325G channels were non-functional, despite biotin-capture in Western blots revealed normal plasma membrane subunit expression. Mutant subunits exerted dominant-negative effects when incorporated into heteromeric channels with Kv7.2 and/or Kv7.3 subunits. Increasing cellular PIP2 levels by co-expression of type 1γ PI(4)P5-kinase (PIP5K) partially recovered homomeric Kv7.2 R325G channel function. Currents carried by heteromeric channels incorporating Kv7.2 R325G subunits were more readily inhibited than wild-type channels upon activation of a voltage-sensitive phosphatase (VSP), and recovered more slowly upon VSP switch-off. These results reveal for the first time that a mutation-induced decrease in current sensitivity to PIP2 is the primary molecular defect responsible for Kv7.2-EE in individuals carrying the R325G variant, further expanding the range of pathogenetic mechanisms exploitable for personalized treatment of Kv7.2-related epilepsies. PMID:27905566

Early-onset epileptic encephalopathy caused by a reduced sensitivity of Kv7.2 potassium channels to phosphatidylinositol 4,5-bisphosphate.

PubMed

Soldovieri, Maria Virginia; Ambrosino, Paolo; Mosca, Ilaria; De Maria, Michela; Moretto, Edoardo; Miceli, Francesco; Alaimo, Alessandro; Iraci, Nunzio; Manocchio, Laura; Medoro, Alessandro; Passafaro, Maria; Taglialatela, Maurizio

2016-12-01

Kv7.2 and Kv7.3 subunits underlie the M-current, a neuronal K + current characterized by an absolute functional requirement for phosphatidylinositol 4,5-bisphosphate (PIP 2 ). Kv7.2 gene mutations cause early-onset neonatal seizures with heterogeneous clinical outcomes, ranging from self-limiting benign familial neonatal seizures to severe early-onset epileptic encephalopathy (Kv7.2-EE). In this study, the biochemical and functional consequences prompted by a recurrent variant (R325G) found independently in four individuals with severe forms of neonatal-onset EE have been investigated. Upon heterologous expression, homomeric Kv7.2 R325G channels were non-functional, despite biotin-capture in Western blots revealed normal plasma membrane subunit expression. Mutant subunits exerted dominant-negative effects when incorporated into heteromeric channels with Kv7.2 and/or Kv7.3 subunits. Increasing cellular PIP 2 levels by co-expression of type 1γ PI(4)P5-kinase (PIP5K) partially recovered homomeric Kv7.2 R325G channel function. Currents carried by heteromeric channels incorporating Kv7.2 R325G subunits were more readily inhibited than wild-type channels upon activation of a voltage-sensitive phosphatase (VSP), and recovered more slowly upon VSP switch-off. These results reveal for the first time that a mutation-induced decrease in current sensitivity to PIP 2 is the primary molecular defect responsible for Kv7.2-EE in individuals carrying the R325G variant, further expanding the range of pathogenetic mechanisms exploitable for personalized treatment of Kv7.2-related epilepsies.

Integration of G protein α (Gα) signaling by the regulator of G protein signaling 14 (RGS14).

PubMed

Brown, Nicole E; Goswami, Devrishi; Branch, Mary Rose; Ramineni, Suneela; Ortlund, Eric A; Griffin, Patrick R; Hepler, John R

2015-04-03

RGS14 contains distinct binding sites for both active (GTP-bound) and inactive (GDP-bound) forms of Gα subunits. The N-terminal regulator of G protein signaling (RGS) domain binds active Gαi/o-GTP, whereas the C-terminal G protein regulatory (GPR) motif binds inactive Gαi1/3-GDP. The molecular basis for how RGS14 binds different activation states of Gα proteins to integrate G protein signaling is unknown. Here we explored the intramolecular communication between the GPR motif and the RGS domain upon G protein binding and examined whether RGS14 can functionally interact with two distinct forms of Gα subunits simultaneously. Using complementary cellular and biochemical approaches, we demonstrate that RGS14 forms a stable complex with inactive Gαi1-GDP at the plasma membrane and that free cytosolic RGS14 is recruited to the plasma membrane by activated Gαo-AlF4(-). Bioluminescence resonance energy transfer studies showed that RGS14 adopts different conformations in live cells when bound to Gα in different activation states. Hydrogen/deuterium exchange mass spectrometry revealed that RGS14 is a very dynamic protein that undergoes allosteric conformational changes when inactive Gαi1-GDP binds the GPR motif. Pure RGS14 forms a ternary complex with Gαo-AlF4(-) and an AlF4(-)-insensitive mutant (G42R) of Gαi1-GDP, as observed by size exclusion chromatography and differential hydrogen/deuterium exchange. Finally, a preformed RGS14·Gαi1-GDP complex exhibits full capacity to stimulate the GTPase activity of Gαo-GTP, demonstrating that RGS14 can functionally engage two distinct forms of Gα subunits simultaneously. Based on these findings, we propose a working model for how RGS14 integrates multiple G protein signals in host CA2 hippocampal neurons to modulate synaptic plasticity. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

41G crew activities

NASA Image and Video Library

2009-06-25

41G-101-039 (5-13 Oct 1984) --- Two members of a record seven-person crew are pictured during Intravehicular Activity (IVA) aboard the Earth-orbiting Space Shuttle Challenger. Hold picture with open hand at right center edge. Astronaut David C. Leestma, mission specialist, is at right observing a test by payload specialist Marc Garneau, representing the National Research Council (NRC) of Canada. Garneau spent much of his on-duty time conducting a series of experiments for the NRC. The crew consisted of astronauts Robert L. Crippen, commander; Jon A. McBride, pilot; mission specialist's Kathryn D. Sullivan, Sally K. Ride, and David D. Leestma; Canadian astronaut Marc Garneau, and Paul D. Scully-Power, payload specialist's. EDITOR'S NOTE: The STS-41G mission had the first American female EVA (Sullivan); first seven-person crew; first orbital fuel transfer; and the first Canadian (Garneau).