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1

Circulating soluble intercellular adhesion molecule-1 in lung cancer: a systematic review  

PubMed Central

Soluble intercellular adhesion molecule-1(sICAM-1) has been implicated in tumor progression and metastasis. However the expression of circulating sICAM-1 as well as its diagnostic and prognostic value in patients with lung cancer remains controversial. We performed an electronic database (including PubMed, Web of Science, and Medline) search with the terms "ICAM", "intercellular adhesion molecule" and "lung cancer", and summarized the results of eligible studies in order to review the expression of sICAM-1 as well as its clinical significance in lung cancer. According to our literature search, we conducted a final analysis of 1258 patients from 16 studies. And we revealed that the circulating concentration of sICAM-1 in lung cancer patients was significantly higher than that in healthy controls. Additionally, baseline sICAM-1 levels apparently were associated with ECOG performance status, gender, histology type and disease stages. Furthermore, there seems to be a significantly inverse association between sICAM-1 levels, prognosis and response rate in NSCLC patients. In conclusion, sICAM-1 appeared to be a potential diagnostic and prognostic biomarker in lung cancer patients. Additional prospective studies are required to confirm this issue.

Gu, Xiaoling; Ma, Chunyan; Yuan, Dongmei

2012-01-01

2

Soluble intercellular adhesion molecule-1 and clinical outcomes in patients with acute lung injury  

PubMed Central

Objective To determine if levels of soluble intercellular adhesion molecule-1 (sICAM-1), a marker of alveolar epithelial and endothelial injury, differ in patients with hydrostatic pulmonary edema and acute lung injury (ALI) and are associated with clinical outcomes in patients with ALI. Design, setting, and participants Measurement of sICAM-1 levels in (1) plasma and edema fluid from 67 patients with either hydrostatic pulmonary edema or ALI enrolled in an observational, prospective single center study, and (2) in plasma from 778 patients with ALI enrolled in a large multi-center randomized controlled trial of ventilator strategy. Results In the single-center study, levels of sICAM-1 were significantly higher in both edema fluid and plasma (median 938 and 545 ng/ml, respectively) from ALI patients compared to hydrostatic edema patients (median 384 and 177 ng/ml, P < 0.03 for both comparisons). In the multi-center study, higher plasma sICAM-1 levels were associated with poor clinical outcomes in both unadjusted and multivariable models. Subjects with ALI whose plasma sICAM-1 levels increased over the first 3 days of the study had a higher risk of death, after adjusting for other important predictors of outcome (odds ratio 1.48; 95% CI 1.03–2.12, P = 0.03). Conclusions Both plasma and edema fluid levels of sICAM-1 are higher in patients with ALI than in patients with hydrostatic pulmonary edema. Higher plasma sICAM-1 levels and increasing sICAM-1 levels over time are associated with poor clinical outcomes in ALI. Measurement of sICAM-1 levels may be useful for identifying patients at highest risk of poor outcomes from ALI. PMID:18670758

Eisner, Mark D.; Parsons, Polly E.; Thompson, B. Taylor; Conner, Edward R.; Matthay, Michael A.; Ware, Lorraine B.

2009-01-01

3

Differential up-regulation of circulating soluble and endothelial cell intercellular adhesion molecule-1 in mice.  

PubMed Central

Although circulating levels of soluble intercellular adhesion molecule-1 (sICAM-1) are frequently used as an indicator of the severity of different immune, inflammatory, or neoplastic diseases, little is known about the factors that govern plasma sICAM-1 concentration and its relationship to the membranous form of ICAM-1 (mICAM-1) expressed on vascular endothelial cells. Plasma sICAM-1 concentration (measured by enzyme-linked immunosorbent assay) and mICAM-1 expression (measured using the dual radiolabeled monoclonal antibody technique) in different vascular beds (eg, lung, small intestine, and spleen) were monitored in wild-type (C57BL) and ICAM-1-deficient mice, before and after administration of tumor necrosis factor (TNF)-alpha. In wild-type mice, TNF-alpha elicited time-dependent increases in lung and intestine mICAM-1 (plateau achieved at 12 hours), with a corresponding increase in plasma sICAM-1 (peaked at 5 hours and then declined). The initial increases in mICAM-1 and pulmonary leukocyte sequestration (measured as lung myeloperoxidase activity) induced by TNF-alpha preceded any detectable elevation in sICAM-1. In ICAM-1-deficient mice, plasma sICAM-1 was reduced by approximately 70%, with > 95% reductions of mICAM-1 in lung and intestine, and > 75% reduction in splenic accumulation of anti-ICAM-1 antibody. Although TNF-alpha doubled plasma sICAM-1 in ICAM-1-deficient mice, mICAM-1 was unaffected in all tissues. Either splenectomy or pretreatment with cycloheximide resulted in an attenuated TNF-induced increase in sICAM-1, without affecting mICAM-1 expression. These findings indicate that plasma sICAM-1 concentration does not accurately reflect the level of ICAM-1 expression on endothelial cells in different vascular beds. PMID:9212746

Komatsu, S.; Flores, S.; Gerritsen, M. E.; Anderson, D. C.; Granger, D. N.

1997-01-01

4

Prognostic value of plasma soluble intercellular adhesion molecule-1 and endothelin-1 concentration in patients with chronic congestive heart failure  

Microsoft Academic Search

We tested the hypothesis that plasma endothelin-1 (ET-1) and soluble intercellular adhesion molecule-1 (sICAM-1) in patients with congestive heart failure (CHF) are related to subsequent survival, and assessed whether the measurements of these substances provide additional prognostic information to that obtained from clinical and biochemical variables previously known to be associated with high mortality. Plasma levels of sICAM-1 and ET-1

Takayoshi Tsutamoto; Tomoko Hisanaga; Daisuke Fukai; Atsuyuki Wada; Yukiharu Maeda; Keiko Maeda; Masahiko Kinoshita

1995-01-01

5

Preanalytical Factors (Biological Variation) and the Measurement of Serum Soluble Intercellular Adhesion Molecule1 in Humans: Influence of the Time of Day, Food Intake, and Physical and Psychological Stress  

Microsoft Academic Search

Background: Adhesion proteins such as soluble inter- cellular adhesion molecule-1 (sICAM-1) may be impor- tant markers for early atherosclerosis and some other diseases. To devise optimum specimen collection proce- dures, we investigated the effects on serum sICAM-1 of several preanalytical factors (factors that occur before a specimen is analyzed) such as the time of the day, food ingestion, and physical

Benoit Dugue; Esa Leppanen; Ralph Grasbeck

6

Association of sICAM-1 and MCP-1 with coronary artery calcification in families enriched for coronary heart disease or hypertension: the NHLBI Family Heart Study  

Microsoft Academic Search

BACKGROUND: Data accumulated from mouse studies and in vitro studies of human arteries support the notion that soluble intercellular adhesion molecule-1 (sICAM-1) and monocyte chemoattractant protein-1 (MCP-1) play important roles in the inflammation process involved in atherosclerosis. However, at the population level, the utility of sICAM-1 and MCP-1 as biomarkers for subclinical atherosclerosis is less clear. In the follow-up exam

Weihong Tang; James S Pankow; J Jeffrey Carr; Russell P Tracy; Suzette J Bielinski; Kari E North; Paul N Hopkins; Aldi T Kraja; Donna K Arnett

2007-01-01

7

Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns  

PubMed Central

Perinatal hypoxia results in neuronal and endothelial cell damage. The main purpose of this study was to investigate the correlation of soluble intercellular adhesion molecule 1 (sICAM-1) expression and peripheral blood changes in perinatal asphyxia with neuronal injury markers in low birth weight (LBW) neonates. We compared the concentrations of serum sICAM-1, neuron-specific enolase (NSE) and antibodies specific for NR2 glutamate receptors in 29 asphyxiated and 20 control infants using standard enzyme immunoassay procedures. The mean total concentrations of sICAM-1 and neuron-specific proteins (NSE and NR2-specific antibodies) were higher in the asphyxiated infants than in the control infants. The serum sICAM-1 concentrations significantly correlated with Apgar scoring and with the pH and lactate data from capillary or arterial cord blood. No significant correlation between serum concentrations of neuron specific proteins and blood changes of asphyxia was found. Therefore, endothelial sICAM-1 expression levels might be accepted as an indicator of the severity of perinatal asphyxia in LBW infants. PMID:25358349

Huseynova, Saadat; Panakhova, Nushaba; Orujova, Pusta; Hasanov, Safikhan; Guliyev, Mehman; Orujov, Agil

2014-01-01

8

Intercellular adhesion molecule-1 is a regulator of blood–testis barrier function  

PubMed Central

Summary The mechanism underlying the movement of preleptotene/leptotene spermatocytes across the blood–testis barrier (BTB) during spermatogenesis is not well understood largely owing to the fact that the BTB, unlike most other blood–tissue barriers, is composed of several co-existing and co-functioning junction types. In the present study, we show that intercellular adhesion molecule-1 [ICAM-1, a Sertoli and germ cell adhesion protein having five immunoglobulin (Ig)-like domains, in addition to transmembrane and cytoplasmic domains] is a regulator of BTB integrity. Initial experiments showed ICAM-1 to co-immunoprecipitate and co-localize with tight junction and basal ectoplasmic specialization proteins such as occludin and N-cadherin, which contribute to BTB function. More importantly, overexpression of ICAM-1 in Sertoli cells in vitro enhanced barrier function when monitored by transepithelial electrical resistance measurements, illustrating that ICAM-1-mediated adhesion can promote BTB integrity. On the other hand, overexpression of a truncated form of ICAM-1 that consisted only of the five Ig-like domains (sICAM-1; this form of ICAM-1 is known to be secreted) elicited an opposite effect when Sertoli cell barrier function was found to be perturbed in vitro; in this case, sICAM-1 overexpression resulted in the downregulation of several BTB constituent proteins, which was probably mediated by Pyk2/p-Pyk2-Y402 and c-Src/p-Src-Y530. These findings were expanded to the in vivo level when BTB function was found to be disrupted following sICAM-1 overexpression. These data illustrate the existence of a unique mechanism in the mammalian testis where ICAM-1 can either positively or negatively regulate BTB function. PMID:22976294

Xiao, Xiang; Cheng, C. Yan; Mruk, Dolores D.

2012-01-01

9

Disturbed Homeostasis of Lung Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 During Sepsis  

PubMed Central

Cecal ligation and puncture (CLP)-induced sepsis in mice was associated with perturbations in vascular adhesion molecules. In CLP mice, lung vascular binding of 125I-monoclonal antibodies to intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 revealed sharp increases in binding of anti-ICAM-1 and significantly reduced binding of anti-VCAM-1. In whole lung homogenates, intense ICAM-1 up-regulation was found (both in mRNA and in protein levels) during sepsis, whereas very little increase in VCAM-1 could be measured although some increased mRNA was found. During CLP soluble VCAM-1 (sVCAM-1) and soluble ICAM-1 (sICAM-1) appeared in the serum. When mouse dermal microvascular endothelial cells (MDMECs) were incubated with serum from CLP mice, constitutive endothelial VCAM-1 fell in association with the appearance of sVCAM-1 in the supernatant fluids. Under the same conditions, ICAM-1 cell content increased in MDMECs. When MDMECs were evaluated for leukocyte adhesion, exposure to CLP serum caused increased adhesion of neutrophils and decreased adhesion of macrophages and T cells. The progressive build-up in lung myeloperoxidase after CLP was ICAM-1-dependent and independent of VLA-4 and VCAM-1. These data suggest that sepsis disturbs endothelial homeostasis, greatly favoring neutrophil adhesion in the lung microvasculature, thereby putting the lung at increased risk of injury. PMID:15039231

Laudes, Ines J.; Guo, Ren-Feng; Riedemann, Niels C.; Speyer, Cecilia; Craig, Ron; Sarma, J. Vidya; Ward, Peter A.

2004-01-01

10

Disturbed homeostasis of lung intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 during sepsis.  

PubMed

Cecal ligation and puncture (CLP)-induced sepsis in mice was associated with perturbations in vascular adhesion molecules. In CLP mice, lung vascular binding of (125)I-monoclonal antibodies to intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 revealed sharp increases in binding of anti-ICAM-1 and significantly reduced binding of anti-VCAM-1. In whole lung homogenates, intense ICAM-1 up-regulation was found (both in mRNA and in protein levels) during sepsis, whereas very little increase in VCAM-1 could be measured although some increased mRNA was found. During CLP soluble VCAM-1 (sVCAM-1) and soluble ICAM-1 (sICAM-1) appeared in the serum. When mouse dermal microvascular endothelial cells (MDMECs) were incubated with serum from CLP mice, constitutive endothelial VCAM-1 fell in association with the appearance of sVCAM-1 in the supernatant fluids. Under the same conditions, ICAM-1 cell content increased in MDMECs. When MDMECs were evaluated for leukocyte adhesion, exposure to CLP serum caused increased adhesion of neutrophils and decreased adhesion of macrophages and T cells. The progressive build-up in lung myeloperoxidase after CLP was ICAM-1-dependent and independent of VLA-4 and VCAM-1. These data suggest that sepsis disturbs endothelial homeostasis, greatly favoring neutrophil adhesion in the lung microvasculature, thereby putting the lung at increased risk of injury. PMID:15039231

Laudes, Ines J; Guo, Ren-Feng; Riedemann, Niels C; Speyer, Cecilia; Craig, Ron; Sarma, J Vidya; Ward, Peter A

2004-04-01

11

Soluble intercellular adhesion molecule 1 and flow-mediated dilatation are related to the estimated risk of coronary heart disease independently from each other  

Microsoft Academic Search

Background: Flow mediated dilatation (FMD) of the brachial artery and soluble intercellular adhesion molecule 1 (sICAM-1) are measures of distinct functions of the endothelium, reflecting nitric oxide (NO)-mediated and pro-inflammatory status, respectively. The comparative value of the two measures in relation to cardiovascular risk is unknown. Objective: To study and quantify the relation between these two measures, and their relative

D. R. Witte; W. M. R. Broekmans; A. F. M. Kardinaal; I. A. A. Klöpping-Ketelaars; G. van Poppel; M. L. Bots; C. Kluft; J. M. G. Princen

2003-01-01

12

Soluble interleukin-2 receptor, intercellular adhesion molecule-1 and interleukin-10 serum levels in patients withelanoma  

PubMed Central

Serum soluble interleukin-2 receptor (sIL-2R), intercellular adhesion molecule-1 (sICAM-1) and interleukin-10 (IL-10) have each been reported as useful markers for melanoma progression. To evaluate the clinical relevance of these three markers, we simultaneously analysed their serum levels in patients with melanoma. A longitudinal study with a 3-year follow-up was performed and different stages of the disease were considered. Mean values of sIL-2R were significantly higher than in normal controls in all stages and correlated with the disease progression. The prognosis of patients with levels > 529 U/ml of sIL-2R was significantly poorer than in patients with sIL-2R levels < 529 U/ml. Levels of sICAM-1 were also elevated in melanoma patients, specially at the time of the metastatic disease. Serum IL-10 levels were more frequently detectable in the patients that developed metastasis during follow-up, and the prognosis of patients with detectable IL-10 levels was significantly poorer than in those patients with IL-10 undetected levels. Statistical analysis based on Logistic and Cox regression models showed that only sex, stage and sIL-2R value are factors significantly associated with metastatic progression. Moreover, high levels of sIL-2R could be a risk factor for malignant progression in melanoma. © 2000 Cancer Research Campaign PMID:10970683

Boyano, M D; Garcia-Vázquez, M D; López-Michelena, T; Gardeazabal, J; Bilbao, J; Cañavate, M L; Galdeano, A García De; Izu, R; Díaz-Ramón, L; Raton, J A; Díaz-Pérez, J L

2000-01-01

13

Increased circulating PEDF and low sICAM-1 are associated with sickle cell retinopathy.  

PubMed

Sickle cell retinopathy (SCR) develops in up to 30% of sickle cell disease patients (SCD) during the second decade of life. Treatment for this affection remains palliative, so studies on its pathophysiology may contribute to the future development of novel therapies. SCR is more frequently observed in hemoglobin SC disease and derives from vaso-occlusion in the microvasculature of the retina leading to neovascularization and, eventually, to blindness. Circulating inflammatory cytokines, angiogenic factors, and their interaction may contribute to the pathophysiology of this complication. Angiopoietin (Ang)-1, Ang-2, soluble vascular cell adhesion molecule-1, intercellular adhesion molecule (ICAM)-1, E-selectin, P-selectin, IL1-?, TNF-?, pigment epithelium derived factor (PEDF) and vascular endothelial growth factor plasmatic levels were determined in 37 SCD patients with retinopathy, 34 without retinopathy, and healthy controls. We observed that sICAM-1 is significantly decreased, whereas PEDF is elevated in HbSC patients with retinopathy (P=0.012 and P=0.031, respectively). Ang-1, Ang-2 and IL1-? levels were elevated in SCD patients (P=0.001, P<0.001 and P=0.001, respectively), compared to controls, and HbSS patients presented higher levels of Ang-2 compared to HbSC (P<0.001). Our study supports the possible influence of sICAM-1 and PEDF on the pathophysiology of retinal neovascularization in SCD patients. PMID:25172543

Cruz, P R S; Lira, R P C; Pereira Filho, S A C; Souza, B B; Mitsuushi, F N; Menaa, F; Fertrin, K Y; Vasconcellos, J P C; Conran, N; Costa, F F; Melo, M B

2015-01-01

14

Dipeptidyl peptidase-4 inhibition with saxagliptin enhanced nitric oxide release and reduced blood pressure and sICAM-1 levels in hypertensive rats.  

PubMed

Most patients with diabetes also have hypertension, a risk factor associated with atherothrombotic disease and characterized by endothelial cell (EC) dysfunction and loss of nitric oxide (NO) bioavailability. Recent studies suggest a possible antihypertensive effect with dipeptidyl peptidase-4 (DPP4) inhibition; however, the underlying mechanism is not understood. In this study, we tested the effects of the DPP4 inhibitor, saxagliptin, on EC function, blood pressure, and soluble intercellular adhesion molecule 1 (sICAM-1) levels in hypertensive rats. Spontaneously hypertensive rats were treated with vehicle or saxagliptin (10 mg·kg(-1)·day(-1)) for 8 weeks. NO and peroxynitrite (ONOO(-)) release from aortic and glomerular ECs was stimulated with calcium ionophore and measured using electrochemical nanosensor technology. Changes in EC function were correlated with fasting glucose levels. Saxagliptin treatment was observed to increase aortic and glomerular NO release by 22% (P < 0.001) and 23% (P < 0.001), respectively, with comparable reductions in ONOO(-) levels; the NO/ONOO(-) ratio increased by >50% in both EC types (P < 0.001) as compared with vehicle. Saxagliptin also reduced mean arterial pressure from 170 ± 10 to 158 ± 10 mm Hg (P < 0.001) and decreased sICAM-1 levels by 37% (P < 0.01). The results of this study suggest that DPP4 inhibition reduces blood pressure and inflammation in hypertensive rats while increasing NO bioavailability. PMID:22932707

Mason, R Preston; Jacob, Robert F; Kubant, Ruslan; Ciszewski, Aleksander; Corbalan, J Jose; Malinski, Tadeusz

2012-11-01

15

Increased Plasma and Endothelial Cell Expression of Chemokines and Adhesion Molecules in Chronic Kidney Disease  

Microsoft Academic Search

Chemokines and adhesion molecules are involved in early events of atherogenesis. In the present study, we investigated the effects of the uremic milieu on the expression of monocyte chemoattractant protein-1 (MCP-1), interleukin-8 (IL-8), soluble vascular adhesion molecule-1 (sVCAM-1) and soluble intercellular adhesion molecule-1 (sICAM-1) and their relationship to cardiovascular status. Plasma samples were obtained from patients in different stages of

A. E. M. Stinghen; S. M. Gonçalves; E. G. Martines; L. S. Nakao; M. C. Riella; C. A. Aita; R. Pecoits-Filho

2009-01-01

16

Intercellular Adhesion Molecule1 Suppression in Skin by Topical Delivery of AntiSense Oligonucleotides  

Microsoft Academic Search

We topically applied 20 nucleotide phosphorothioate intercellular adhesion molecule-1 anti-sense oligodeoxynucleotide in a cream formulation. It effectively inhibited tumor necrosis factor-?-induced expression of intercellular adhesion molecule-1 in human skin transplanted on severe compromised immunodeficient mice. The effects were concentration dependent, sequence specific, and resulted from reduction of intercellular adhesion molecule-1 mRNA levels in the skin. Intravenous administration of the drug

Rahul C. Mehta; Kimberly K. Stecker; Scott R. Cooper; Michael V. Templin; Yali J. Tsai; Thomas P. Condon; C. Frank Bennett; Gregory E. Hardee

2000-01-01

17

Rational Design of Intercellular Adhesion Molecule-1 (ICAM-1) Variants for Antagonizing Integrin Lymphocyte Function-  

E-print Network

Rational Design of Intercellular Adhesion Molecule-1 (ICAM-1) Variants for Antagonizing Integrin between integrin lymphocyte function-associated antigen-1 (LFA-1) and its ligand intercellular adhesion ligand for lymphocyte function- associated antigen-1 (LFA-1), a member of the integrin family of adhesion

Baker, David

18

Circulating intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin in patients with type 2 diabetes mellitus  

Microsoft Academic Search

Serum levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-selectin in patients with type 2 diabetes mellitus (n=64) and control subjects (n=40) were studied. Serum ICAM-1 concentrations in diabetic patients were significantly higher than those of control subjects (378.2±70.0 versus 220.4±31.8 ng\\/ml, P<0.01). By multiple regression analysis, hemoglobin A1c was independently associated with serum ICAM-1 concentration

Seijiro Kado; Naokazu Nagata

1999-01-01

19

Expression of cell adhesion molecules in laryngeal carcinoma – preliminary analysis  

PubMed Central

Aim of the study Intercellular adhesion molecules present in immunocompetent cells as well as endothelium and tumour cells can regulate cell migration, angiogenesis, apoptosis, proliferation, and metastases in solid tumours. The aim of this study was to analyse the sICAM-1 (soluble intercellular adhesion molecule 1) and sVCAM-1 (soluble vascular cell adhesion molecule 1) expression in peripheral blood mononuclear cell (PBMC) cultures, and to find their relationships with clinicomorphological characteristics in laryngeal cancer. Materials and methods The analysis included a group of 50 patients with verified squamous cell carcinoma of the larynx. The control group constituted 30 healthy volunteers. The pathological assessment included pTNM, stage, histological grade, and type of invasion according to the tumour front grading. The expression of adhesion molecules was assessed using the enzyme-linked immunosorbent assay (ELISA). Results Increased expression of sICAM-1 and sVCAM-1 was an indicator of more aggressive laryngeal carcinomas. More advanced local changes evaluated on the pT feature were connected with a higher sVCAM-1 (p = 0.017), but not sICAM-1 level. The presence of lymph node metastases correlated with a higher expression of adhesion molecules (p = 0.012 and p = 0.003, for sICAM-1 and sVCAM-1, respectively). Tumours with more diffuse growth and infiltrating with small cell groups (< 15/hpf) was characterised by the highest level of adhesive proteins (p = 0.001 and p = 0.02 for sICAM and sVCAM, respectively). Moreover, lower levels of sICAM-1 and sVCAM-1 were observed more frequently in patients who lived longer than five years after treatment. Conclusions The study indicates the importance of the sICAM and sVCAM expression as indicators of advanced changes and prognosis in patients with laryngeal carcinoma. PMID:25784838

Morawski, Piotr; Kopta, Renata; Mochocki, Marcin; Brzezi?ska-B?aszczyk, Ewa; Lewy-Trenda, Iwona

2014-01-01

20

Intercellular Adhesion Molecule1 (ICAM-1) in the Pathogenesis of Asthma  

Microsoft Academic Search

Airway eosinophilia, epithelial desquamation, and hyperresponsiveness are characteristics of the airway inflammation underlying bronchial asthma. The contribution of intercellular adhesion molecule-1 (ICAM-1) to eosinophil migration and airway responsiveness was studied. ICAM-1 partially mediated eosinophil adhesion to endothelium in vitro and was upregulated on inflamed bronchial endothelium in vivo. ICAM-1 expression was also upregulated on inflamed airway epithelium in vitro and

Craig D. Wesgner; Robert H. Gundel; Patricia Reilly; Nancy Haynes; L. Gordon Letts; Robert Rothlein

1990-01-01

21

Detection of Vascular Adhesion Molecule1 Expression Using a Novel Multimodal Nanoparticle  

Microsoft Academic Search

Endothelial vascular adhesion molecule-1 (VCAM-1) is a critical component of the leukocyte-endothelial adhesion cascade, and its strict temporal and spatial regulation make it an ideal target for imaging and therapy. The goal of this study was to develop novel VCAM-1-targeted imaging agents detectable by MRI and fluorescence imaging using phage display-derived peptide sequences and multimodal nanoparticles (NPs). We hypothesized that

Kimberly A. Kelly; Jennifer R. Allport; Andrew Tsourkas; Vivek R. Shinde-Patil; Lee Josephson; Ralph Weissleder

2010-01-01

22

Inflammatory and Immune Responses are Impaired in Mice Deficient in Intercellular Adhesion Molecule 1  

Microsoft Academic Search

Gene targeting was used to produce mice deficient in intercellular adhesion molecule 1 (ICAM-1) or CD54, an immunoglobulin-like cell adhesion molecule that binds beta_2 integrins. Homozygous deficient animals develop normally, are fertile, and have a moderate granulocytosis. The nature of the mutation, RNA analysis, and immunostaining are consistent with complete loss of surface expression of ICAM-1. Deficient mice exhibit prominent

James E. Sligh Jr.; Christie M. Ballantyne; Susan S. Rich; Hal K. Hawkins; C. Wayne Smith; Alan Bradley; Arthur L. Beaudet

1993-01-01

23

Intercellular Adhesion Molecule 1 Mediates Mononuclear Cell Infiltration into Rat Glomeruli after Renal Ablation  

Microsoft Academic Search

Mononuclear cells, primarily macrophages and lymphocytes, infiltrate the renal glomeruli and are involved in the progression of various glomerular diseases. Intercellular adhesion molecule 1 (ICAM-1) is expressed on the vascular endothelium and mediates the infiltration of leukocytes into the site of inflammation. Although the expression of ICAM-1 can be induced by the stimulation of inflammatory cytokine, ICAM-1 expression can also

Nobuyuki Miyatake; Kenichi Shikata; Hikaru Sugimoto; Masahiko Kushiro; Yasushi Shikata; Saeko Ogawa; Yoshiko Hayashi; Masayuki Miyasaka; Hirofumi Makino

1998-01-01

24

Intercellular adhesion molecule 1 gene associations with immunologic subsets of inflammatory bowel disease  

Microsoft Academic Search

Background & Aims: Inflammatory bowel disease is characterized by a failure to down-regulate the usual self-limited gut inflammatory response, suggesting that one or more of the predisposing genes could be those that determine the level of the immune response along the inflammatory pathway. The aim of this study was to examine potential associations of intercellular adhesion molecule 1 (ICAM-1) gene

Huiying Yang; Devendra K. Vora; Stephan R. Targan; Hiroo Toyoda; Arthur L. Beaudet; Jerome I. Rotter

1995-01-01

25

Elevated Serum Vascular Cell Adhesion Molecule-1 Is Associated with Septic Encephalopathy in Adult Community-Onset Severe Sepsis Patients  

PubMed Central

Background and Aim. Septic encephalopathy (SE) is a common complication of severe sepsis. Increased concentrations of circulating soluble adhesion molecules are reported in septic patients. This study aimed to determine whether serum adhesion molecules are associated with SE. Methods. Seventy nontraumatic, nonsurgical adult patients with severe sepsis admitted through ER were evaluated. Serum adhesion molecules were assessed for their relationship with SE, and compared with other clinical predictors and biomarkers. Results. Twenty-three (32.8%) patients had SE. SE group had higher in-hospital mortality (40% versus 11%, P = 0.009) and their sVCAM-1, sICAM-1, and lactate levels on admission were also higher than non-SE group. By stepwise logistic regression model, sVCAM-1, age, and maximum 24-hours SOFA score were independently associated with septic encephalopathy. The AUC analysis of ROC curve of different biomarkers showed that sVCAM-1 is better to predict SE. The sVCAM-1 levels in the SE group were significantly higher than those of the non-SE group at three time periods (Days 1, 4, and 7). Conclusions. Septic encephalopathy implies higher mortality in nontraumatic, nonsurgical patients with severe sepsis. VCAM-1 level on presentation is a more powerful predictor of SE in these patients than lactate concentration and other adhesion molecules on admission. PMID:24883317

Su, Chih-Min; Cheng, Hsien-Hung; Tsai, Tsung-Cheng; Hsiao, Sheng-Yuan; Tsai, Nai-Wen; Chang, Wen-Neng; Lin, Wei-Che; Cheng, Ben-Chung; Chang, Ya-Ting; Chiang, Yi-Fang; Kung, Chia-Te; Lu, Cheng-Hsien

2014-01-01

26

Intercellular Adhesion Molecule-1 (ICAM-1) in the Pathogenesis of Asthma  

NASA Astrophysics Data System (ADS)

Airway eosinophilia, epithelial desquamation, and hyperresponsiveness are characteristics of the airway inflammation underlying bronchial asthma. The contribution of intercellular adhesion molecule-1 (ICAM-1) to eosinophil migration and airway responsiveness was studied. ICAM-1 partially mediated eosinophil adhesion to endothelium in vitro and was upregulated on inflamed bronchial endothelium in vivo. ICAM-1 expression was also upregulated on inflamed airway epithelium in vitro and in vivo. In a primate model of asthma, a monoclonal antibody to ICAM-1 attenuated airway eosinophilia and hyperresponsiveness. Thus, antagonism of ICAM-1 may provide a therapeutic approach to reducing airway inflammation, hyperresponsiveness, and asthma symptoms.

Wesgner, Craig D.; Gundel, Robert H.; Reilly, Patricia; Haynes, Nancy; Letts, L. Gordon; Rothlein, Robert

1990-01-01

27

Intercellular adhesion molecule-1 K469E gene polymorphism and Alzheimer’s disease  

Microsoft Academic Search

Inflammatory processes are considered important in the pathogenesis of Alzheimer’s disease (AD). Intercellular adhesion molecule-1 (ICAM-1) is an important mediator of inflammatory response and immune cell activation, is expressed on cerebrovascular endothelium and neuritic plaques in brain of AD patients, and seems to be implicated in the process of neuro-degeneration. A common polymorphism of the ICAM-1 gene (K469E) has been

Roberto Pola; Andrea Flex; Eleonora Gaetani; Angelo Santoliquido; Michele Serricchio; Paolo Pola; Roberto Bernabei

2003-01-01

28

Circulating Intracellular Adhesion Molecule1 Concentrations following Bronchial Provocation in Atopic Asthma  

Microsoft Academic Search

A house dust bronchial provocation test (BPT) was used to investigate the effect of allergen-induced airway inflammation and airway hyperresponsiveness (AHR) on the level of circulating intracellular adhesion molecule-1 (c-ICAM-1). The concentration of c-ICAM-1 was measured by the sandwich ELISA while the level of eosinophil cationic protein (ECP) in the sputum was determined by RIA. The parameter used for quantification

Yoshiki Gonokami; Shin-ichi Konno; Masatsugu Kurokawa; Kenta Kawazu; Kana Ueno; Ken Tomita; Mina Ike; Miki Nyui; Mitsuru Adachi

1997-01-01

29

Indomethacin induced gastropathy in CD18, intercellular adhesion molecule 1, or P-selectin deficient mice  

PubMed Central

BACKGROUND—Neutrophil-endothelial cell interactions are thought to play a critical role in the pathophysiology of non-steroidal anti-inflammatory drug (NSAID) induced gastropathy.?AIMS—To optimise a mouse model of NSAID induced gastropathy and to evaluate the importance of adhesion molecules using adhesion molecule deficient mice.?METHODS—Gastropathy was induced in C57BL/6 mice or their adhesion molecule deficient counterparts via oral administration of indomethacin (20 mg/kg). Lesion scores, mucosal permeability, and histopathology were used to assess gastric mucosal injury.?RESULTS—Intragastric administration of indomethacin induced linear haemorrhagic mucosal lesions, primarily in the corpus of the stomach that were first observed at six hours. These lesions continued to develop over the next six hours with maximal lesion scores and mucosal permeabilities at 12 hours. When indomethacin was administered to mice deficient in CD18, intercellular adhesion molecule 1 (ICAM-1), or P-selectin, there were significant decreases in lesion scores compared with their C57BL/6 controls. In addition, mucosal permeabilities were found to be significantly lower in CD18 or ICAM-1 deficient mice observed at 12 hours.?CONCLUSION—Certain leucocyte and endothelial cell adhesion molecules are important determinants for full expression of indomethacin induced gastropathy. It is proposed that this modification of the mouse model may be useful for the investigation of other pathophysiological mechanisms of NSAID induced gastropathy.???Keywords: indomethacin; gastropathy; cyclooxygenase; intercellular adhesion molecule; VCAM; vascular cell adhesion molecule; P-selectin PMID:10486359

Morise, Z; Granger, D; Fuseler, J; Anderson, D; Grisham, M

1999-01-01

30

Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.  

PubMed

We previously demonstrated that the expression of intercellular adhesion molecule-1 (ICAM-1) by skeletal muscle cells after muscle overload contributes to ensuing regenerative and hypertrophic processes in skeletal muscle. The objective of the present study is to reveal mechanisms through which skeletal muscle cell expression of ICAM-1 augments regenerative and hypertrophic processes of myogenesis. This was accomplished by genetically engineering C2C12 myoblasts to stably express ICAM-1, and by inhibiting the adhesive and signaling functions of ICAM-1 through the use of a neutralizing antibody or cell penetrating peptide, respectively. Expression of ICAM-1 by cultured skeletal muscle cells augmented myoblast-myoblast adhesion, myotube formation, myonuclear number, myotube alignment, myotube-myotube fusion, and myotube size without influencing the ability of myoblasts to proliferate or differentiate. ICAM-1 augmented myotube formation, myonuclear accretion, and myotube alignment through a mechanism involving adhesion-induced activation of ICAM-1 signaling, as these dependent measures were reduced via antibody and peptide inhibition of ICAM-1. The adhesive and signaling functions of ICAM-1 also facilitated myotube hypertrophy through a mechanism involving myotube-myotube fusion, protein synthesis, and Akt/p70s6k signaling. Our findings demonstrate that ICAM-1 expression by skeletal muscle cells augments myogenesis, and establish a novel mechanism through which the inflammatory response facilitates growth processes in skeletal muscle. PMID:25281303

Goh, Qingnian; Dearth, Christopher L; Corbett, Jacob T; Pierre, Philippe; Chadee, Deborah N; Pizza, Francis X

2015-02-15

31

The Effect of Iron Treatment on Adhesion Molecules in Patients with Iron Deficiency Anemia  

Microsoft Academic Search

The present study was aimed to determine the effect of iron supplementation on levels of soluble intercellular adhesion molecule-1\\u000a (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1) in patients with iron deficiency anemia (IDA). In this study,\\u000a 26 female patients diagnosed with iron deficiency were treated approximately 3 months of oral iron supplementation (99?±?10 days;\\u000a ferrous glycine sulfate; 100 mg\\/day of elemental iron).

Arif Yuksel; Levent Kebapcilar; Erkan Erdur; Giray Bozkaya; Ismail Sari; Ahmet Alacacioglu; Ayse Gul Kebapcilar; Gulten Sop

2010-01-01

32

Toxoplasma gondii Tachyzoites Cross Retinal Endothelium Assisted by Intercellular Adhesion Molecule-1 In Vitro  

PubMed Central

Retinal infection is the most common clinical manifestation of toxoplasmosis. The route by which circulating Toxoplasma gondii tachyzoites cross the vascular endothelium to enter the human retina is unknown. Convincing studies using murine encephalitis models have strongly implicated leukocyte taxis as one pathway used by the parasite to access target organs. To establish whether tachyzoites might also interact directly with vascular endothelium, we populated a transwell system with human ocular endothelial cells. Human retinal endothelial monolayers permitted transmigration of tachyzoites of RH and three natural isolate strains. Antibody blockade of intercellular adhesion molecule-1 significantly reduced this migration, but did not impact tachyzoite movement across an endothelial monolayer derived from the choroid, which lies adjacent to the retina within the eye. In demonstrating that tachyzoites are capable of independent migration across human vascular endothelium in vitro, this study carries implications for the development of therapeutics aimed at preventing access of tachyzoites to the retina. PMID:22525368

Furtado, João M.; Bharadwaj, Arpita S.; Chipps, Timothy J.; Pan, Yuzhen; Ashander, Liam M.; Smith, Justine R.

2012-01-01

33

Engineering of Single Ig Superfamily Domain of Intercellular Adhesion Molecule 1 (ICAM-1) for Native Fold and Function*S  

E-print Network

Engineering of Single Ig Superfamily Domain of Intercellular Adhesion Molecule 1 (ICAM-1, Boston, Massachusetts 02115 The immunoglobulin (Ig) superfamily is one of the largest families) contains five extracellular Ig superfamily domains (D1-D5) of which the first domain, D1, is the binding

Springer, Timothy A.

34

Effects of intestinal stasis on intercellular adhesion molecule 1 expression in the rat: Role of enteric bacteria  

Microsoft Academic Search

BACKGROUND & AIMS: The mechanisms underlying the inflammatory changes associated with intestinal stasis are poorly understood. The objective of this study was to assess whether endothelial expression of intercellular adhesion molecule 1 (ICAM-1) and leukocyte recruitment are altered after intestinal stasis. METHODS: ICAM-1 expression and granulocyte recruitment were quantified in different tissues of Sprague- Dawley rats using the double-radiolabeled monoclonal

S Komatsu; J Panes; MB Grisham; JM Russell; N Mori; DN Granger

1997-01-01

35

Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle.  

PubMed

Endothelial vascular adhesion molecule-1 (VCAM-1) is a critical component of the leukocyte-endothelial adhesion cascade, and its strict temporal and spatial regulation make it an ideal target for imaging and therapy. The goal of this study was to develop novel VCAM-1-targeted imaging agents detectable by MRI and fluorescence imaging using phage display-derived peptide sequences and multimodal nanoparticles (NPs). We hypothesized that VCAM-1-mediated cell internalization of phage display-selected peptides could be harnessed as an amplification strategy to chaperone and trap imaging agents inside VCAM-1-expressing cells, thus improving target-to-background ratios. To accomplish our goal, iterative phage display was performed on murine endothelium under physiological flow conditions to identify a family of VCAM-1-mediated cell-internalizing peptides. One specific sequence, containing the VHSPNKK motif that has homology to the alpha-chain of very late antigen (a known ligand for VCAM-1), was shown to bind VCAM-1 and block leukocyte-endothelial interactions. Compared with VCAM-1 monoclonal antibody, the peptide showed 12-fold higher target-to-background ratios. A VHSPNKK-modified magnetofluorescent NP (VNP) showed high affinity for endothelial cells expressing VCAM-1 but surprisingly low affinity for macrophages. In contrast, a control NP without VCAM-1-targeting sequences showed no affinity for endothelial cells. In vivo, VNP successfully identified VCAM-1-expressing endothelial cells in a murine tumor necrosis factor-alpha-induced inflammatory model and colocalized with VCAM-1-expressing cells in atherosclerotic lesions present in cholesterol-fed apolipoprotein E apoE-/- mice. These results indicate that: (1) small peptide sequences can significantly alter targeting of NPs, (2) the used amplification strategy of internalization results in high target-to-background ratios, and (3) this technology is useful for in vivo imaging of endothelial markers. PMID:15653572

Kelly, Kimberly A; Allport, Jennifer R; Tsourkas, Andrew; Shinde-Patil, Vivek R; Josephson, Lee; Weissleder, Ralph

2005-02-18

36

Association of serum levels of intercellular adhesion molecule-1 and interleukin-6 with migraine.  

PubMed

To investigate the associations of serum levels of intercellular adhesion molecule-1 (ICAM1) and the pro-inflammatory cytokine interleukin-6 (IL-6) with migraine and migraine subtypes, and to study their correlation with each other in this condition. We used enzyme-linked immunosorbent assay to measure serum levels of ICAM1 and IL-6 in 103 migraine patients with and without aura, in both attack and pain-free periods, and in 100 healthy control subjects. Serum levels of ICAM1 and IL-6 were significantly higher in migraine patients during attacks than in controls (p < 0.05). Serum ICAM1 levels were significantly higher in migraine with aura (MA) than in migraine without aura (MO), (p < 0.05). Correlation analysis indicated a significant positive correlation between serum levels of ICAM1 and IL-6 (p < 0.05) in migraine patients during attacks. Our results indicate that ICAM1 and IL-6 are involved in the pathogenesis of migraine attacks, possibly via an interactive mechanism. PMID:25417066

Wang, Fengzhi; He, Qiu; Ren, Zhanxiu; Li, Fuyong; Chen, Wei; Lin, Xiang; Zhang, Hemin; Tai, Guofeng

2015-04-01

37

Increased Osteoclastogenesis in Mice Lacking the Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1  

PubMed Central

Alterations in bone remodeling are a major public health issue, as therapeutic options for widespread bone disorders such as osteoporosis and tumor-induced osteolysis are still limited. Therefore, a detailed understanding of the regulatory mechanism governing bone cell differentiation in health and disease are of utmost clinical importance. Here we report a novel function of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), a member of the immunoglobulin superfamily involved in inflammation and tumorigenesis, in the physiologic regulation of bone remodeling. Assessing the expression of all members of the murine Ceacam family in bone tissue and marrow, we found CEACAM1 and CEACAM10 to be differentially expressed in both bone-forming osteoblasts and bone-resorbing osteoclasts. While Ceacam10-deficient mice displayed no alteration in structural bone parameters, static histomorphometry demonstrated a reduced trabecular bone mass in mice lacking CEACAM1. Furthermore, cellular and dynamic histomorphometry revealed an increased osteoclast formation in Ceacam1-deficient mice, while osteoblast parameters and the bone formation rate remained unchanged. In line with these findings, we detected accelerated osteoclastogenesis in Ceacam1-deficient bone marrow cells, while osteoblast differentiation, as determined by mineralization and alkaline phosphatase assays, was not affected. Therefore, our results provide in vivo and in vitro evidence for a physiologic role of CEACAM1 in the regulation of osteoclastogenesis. PMID:25490771

Jeschke, Anke; Seitz, Sebastian; Schulze, Jochen; Ito, Wulf D.; Zimmermann, Wolfgang; Amling, Michael; Schinke, Thorsten; Horst, Andrea Kristina; Keller, Johannes

2014-01-01

38

Intercellular adhesion molecule?1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps.  

PubMed

Nasal polyposis (NP) is characterized by chronic mucosal inflammation with infiltrating eosinophils. Eosinophil?mediated tissue remodeling may be involved in NP pathogenesis; therefore, improved understanding of tissue remodeling may result the identification of novel pathways and therapeutic strategies. The present study aimed to investigate the pathological changes occurring during tissue remodeling in NP, in order to assess the role of intercellular adhesion molecule?1 (ICAM?1) in localized tissue remodeling and the potential association between ICAM?1 expression and markers of eosinophil activation. A total of 28 eligible patients and 10 healthy controls participated in the current study. Nasal mucosal tissues of these subjects were retrospectively evaluated for mucosal remodeling using histopathological staining. ICAM?1 and eosinophil cationic protein (ECP) expression levels were determined by immunohistochemical analysis. Compared with the healthy controls, all the specimens from NP patients presented substantial epithelial damage, skewed cellular distribution with a reduced density of goblet cells, an increased density of subepithelial gland and increased subepithelial collagen deposition. In addition, the NP specimens exhibited significantly higher eosinophil infiltration and ICAM?1 expression compared with the controls. Positive correlations were observed between ICAM?1 and ECP expression levels (P=0.010), as well as between extracellular collagen deposition and ICAM?1 (P=0.010) and ECP (P=0.012) expression levels in the NP specimens, but not in the control specimens. Morphological evidence demonstrated eosinophil?mediated tissue remodeling in NP tissues. ICAM?1 expression in activated eosinophils was associated with NP remodeling, indicating the possibility that ICAM?1 may regulate NP remodeling. PMID:25573100

Xin, Jingwei; Sun, Hui; Kong, Hong; Li, Lin; Zheng, Jun; Yin, Chunxia; Cao, Yang; Jia, Yunxiao; Li, Chaoxu

2015-05-01

39

Junctional Adhesion Molecule 1 Is a Functional Receptor for Feline Calicivirus  

PubMed Central

The life cycle of calicivirus is not fully understood because most of the viruses cannot be propagated in tissue culture cells. We studied the mechanism of calicivirus entry into cells using feline calicivirus (FCV), a cultivable calicivirus. From the cDNA library of Crandell-Rees feline kidney (CRFK) cells, feline junctional adhesion molecule 1 (JAM-1), an immunoglobulin-like protein present in tight junctions, was identified as a cellular-binding molecule of the FCV F4 strain, a prototype strain in Japan. Feline JAM-1 expression in nonpermissive hamster lung cells led to binding and infection by F4 and all other strains tested. An anti-feline JAM-1 antibody reduced the binding of FCV to permissive CRFK cells and strongly suppressed the cytopathic effect (CPE) and FCV progeny production in infected cells. Some strains of FCV, such as F4 and F25, have the ability to replicate in Vero cells. We found that regardless of replication ability, FCV bound to Vero and 293T cells via simian and human JAM-1, respectively. In Vero cells, an anti-human JAM-1 antibody inhibited binding, CPE, and progeny production by F4 and F25. In addition, feline JAM-1 expression permitted FCV infection in 293T cells. Taken together, our results demonstrate that feline JAM-1 is a functional receptor for FCV, simian JAM-1 also functions as a receptor for some strains of FCV, and the interaction between FCV and JAM-1 molecules may be a determinant of viral tropism. This is the first report concerning a functional receptor for the viruses in the family Caliciviridae. PMID:16611908

Makino, Akiko; Shimojima, Masayuki; Miyazawa, Takayuki; Kato, Kentaro; Tohya, Yukinobu; Akashi, Hiroomi

2006-01-01

40

Intercellular adhesion molecule-1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps  

PubMed Central

Nasal polyposis (NP) is characterized by chronic mucosal inflammation with infiltrating eosinophils. Eosinophil-mediated tissue remodeling may be involved in NP pathogenesis; therefore, improved understanding of tissue remodeling may result the identification of novel pathways and therapeutic strategies. The present study aimed to investigate the pathological changes occurring during tissue remodeling in NP, in order to assess the role of intercellular adhesion molecule-1 (ICAM-1) in localized tissue remodeling and the potential association between ICAM-1 expression and markers of eosinophil activation. A total of 28 eligible patients and 10 healthy controls participated in the current study. Nasal mucosal tissues of these subjects were retrospectively evaluated for mucosal remodeling using histopathological staining. ICAM-1 and eosinophil cationic protein (ECP) expression levels were determined by immunohistochemical analysis. Compared with the healthy controls, all the specimens from NP patients presented substantial epithelial damage, skewed cellular distribution with a reduced density of goblet cells, an increased density of subepithelial gland and increased subepithelial collagen deposition. In addition, the NP specimens exhibited significantly higher eosinophil infiltration and ICAM-1 expression compared with the controls. Positive correlations were observed between ICAM-1 and ECP expression levels (P=0.010), as well as between extracellular collagen deposition and ICAM-1 (P=0.010) and ECP (P=0.012) expression levels in the NP specimens, but not in the control specimens. Morphological evidence demonstrated eosinophil-mediated tissue remodeling in NP tissues. ICAM-1 expression in activated eosinophils was associated with NP remodeling, indicating the possibility that ICAM-1 may regulate NP remodeling. PMID:25573100

XIN, JINGWEI; SUN, HUI; KONG, HONG; LI, LIN; ZHENG, JUN; YIN, CHUNXIA; CAO, YANG; JIA, YUNXIAO; LI, CHAOXU

2015-01-01

41

Cyclic Stretching of Mesangial Cells Up-Regulates Intercellular Adhesion Molecule-1 and Leukocyte Adherence  

PubMed Central

Intraglomerular hypertension is a primary causal factor in the progressive glomerulosclerosis that characterizes diabetic nephropathy or severe renal ablation. However, inflammation of the glomerular mesangium also participates in at least the early phase of these diseases. In glomerulonephritis, where inflammation is thought to be the predominant causal factor, intraglomerular hypertension is also often present. Mesangial cells (MCs) are critical in orchestrating key functions of the glomerulus including extracellular matrix metabolism, cytokine production, and interaction with leukocytes. Because MCs are subject to increased stretching when intraglomerular hypertension is present, and in glomerulonephritis MC/leukocyte interactions seem to be mediated primarily via the up-regulation of intercellular adhesion molecule-1 (ICAM-1), we examine the possibility that cyclic stretching is a stimulus for increased MC ICAM-1 activity. We demonstrate that the normal low levels of MC ICAM-1 mRNA and protein are dramatically up-regulated by even short intervals of cyclic stretch. This effect is dose- and time-dependent, and requires little amplitude and a brief period of elongation for significant induction. Stretch-induced MC ICAM-1 also leads to a marked elevation in phagocytic leukocyte adherence. This stimulated adherence is equal or greater than that induced by the inflammatory cytokine tumor necrosis factor-?, whereas an additive effect occurs when both are applied in combination. Our results indicate that stretch-induced ICAM-1 may provide a direct link between hypertension and inflammation in the progression of injury and glomerulosclerosis in diabetes, renal ablation, and other forms of glomerulonephritis. PMID:11141473

Riser, Bruce L.; Varani, James; Cortes, Pedro; Yee, Jerry; Dame, Michael; Sharba, Abdul K.

2001-01-01

42

Association of intercellular adhesion molecule 1 with the multichain high-affinity interleukin 2 receptor.  

PubMed Central

Previously, using flow cytometric resonance energy transfer and lateral diffusion measurements, we demonstrated that a 95-kDa protein identified by two monoclonal antibodies (OKT27 and OKT27b) interacts physically with the 55-kDa alpha protein of the high-affinity interleukin 2 (IL-2) receptor. In the present study, this 95-kDa protein (p95) was purified and amino acid sequence data were obtained that showed strong homology to the human intercellular adhesion molecule 1 (ICAM-1). The identity of the p95 protein with ICAM-1 was confirmed by sequential immunoprecipitations using OKT27 and an antibody, WEHI-CAM-1, that is directed toward ICAM-1. We confirmed the physical proximity of p95/ICAM-1 to the IL-2 receptor alpha subunit by demonstrating that radiolabeled IL-2 could be cross-linked to this protein expressed on activated T cells. In functional studies, the antibodies OKT27 and OKT27b inhibited T-cell proliferative responses to OKT3, to soluble antigen, and to heterologous cells (mixed lymphocyte reaction). However, these antibodies did not inhibit IL-2-induced proliferation of an IL-2-dependent T-cell line. Taken together with our previous observations, the present studies suggest that ICAM-1 is in proximity and interacts physically with the high-affinity IL-2 receptor. The association of ICAM-1 with the IL-2 receptor may facilitate the paracrine IL-2-mediated stimulation of T cells expressing IL-2 receptors by augmenting homotypic T-T-cell interaction, by receptor-directed focusing of IL-2 release by helper T cells, and by focusing IL-2 receptors of the physically linked cells to the site of lymphocyte function-associated antigen 1-ICAM-1-IL-2 receptor interaction. Images PMID:1976256

Burton, J; Goldman, C K; Rao, P; Moos, M; Waldmann, T A

1990-01-01

43

Vascular Cellular Adhesion Molecule1 (VCAM-1) Expression in Mice Retinal Vessels Is Affected by Both Hyperglycemia and Hyperlipidemia  

Microsoft Academic Search

BackgroundInflammation has been proposed to be important in the pathogenesis of diabetic retinopathy. An early feature of inflammation is the release of cytokines leading to increased expression of endothelial activation markers such as vascular cellular adhesion molecule-1 (VCAM-1). Here we investigated the impact of diabetes and dyslipidemia on VCAM-1 expression in mouse retinal vessels, as well as the potential role

Carin Gustavsson; Carl-David Agardh; Anna V. Zetterqvist; Jan Nilsson; Elisabet Agardh; Maria F. Gomez; Bernadette Breant

2010-01-01

44

Expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in acute and chronic inflammation  

Microsoft Academic Search

The objective of this study was to quan- tify, in vivo, constitutive and tumor necrosis factor a (TNF-a)-enhanced expression of mucosal ad- dressin cell adhesion molecule-1 (MAdCAM-1) in different tissues from healthy wild-type mice (C57BL\\/6) as well as interleukin-10 (IL-10)- deficient mice with and without active colitis. Using the dual radiolabel monoclonal antibody tech- nique, we found substantial constitutive expression

Elaine M. Connor; Michael J. Eppihimer; Zenichi Morise; D. Neil Granger; Matthew B. Grisham

45

Arsenite enhances tumor necrosis factor-{alpha}-induced expression of vascular cell adhesion molecule-1  

SciTech Connect

Epidemiological studies demonstrated a high association of vascular diseases with arsenite exposure. We hypothesize that arsenite potentiates the effect of proinflammatory cytokines on vascular endothelial cells, and hence contributes to atherosclerosis. In this study, we investigated the effect of arsenite and its induction of glutathione (GSH) on vascular cell adhesion molecule-1 (VCAM-1) protein expression in human umbilical vein endothelial cells (HUVECs) in response to tumor necrosis factor-{alpha} (TNF-{alpha}), a typical proinflammatory cytokine. Our study demonstrated that arsenite pretreatment potentiated the TNF-{alpha}-induced VCAM-1 expression with up-regulations of both activator protein-1 (AP-1) and nuclear factor-{kappa}B (NF-{kappa}B). To elucidate the role of GSH in regulation of AP-1, NF-{kappa}B, and VCAM-1 expression, we employed L-buthionine (S,R)-sulfoximine (BSO), a specific {gamma}-glutamylcysteine synthetase ({gamma}-GCS) inhibitor, to block intracellular GSH synthesis. Our investigation revealed that, by depleting GSH, arsenite attenuated the TNF-{alpha}-induced VCAM-1 expression as well as a potentiation of AP-1 and an attenuation of NF-{kappa}B activations by TNF-{alpha}. Moreover, we found that depletion of GSH would also attenuate the TNF-{alpha}-induced VCAM-1 expression with a down-regulation of the TNF-{alpha}-induced NF-{kappa}B activation and without significant effect on AP-1. On the other hand, the TNF-{alpha}-induced VCAM-1 expression could be completely abolished by inhibition of AP-1 or NF-{kappa}B activity, suggesting that activation of both AP-1 and NF-{kappa}B was necessary for VCAM-1 expression. In summary, we demonstrate that arsenite enhances the TNF-{alpha}-induced VCAM-1 expression in HUVECs via regulation of AP-1 and NF-{kappa}B activities in a GSH-sensitive manner. Our present study suggested a potential mechanism for arsenite in the induction of vascular inflammation and vascular diseases via modulating the actions of proinflammatory cytokines.

Tsou, T.-C. [Division of Environmental Health and Occupational Medicine, National Health Research Institutes, 100 Shih-Chuan 1st Road, Kaohsiung 807, Taiwan (China)]. E-mail: tctsou@nhri.org.tw; Yeh, Szu Ching [Division of Environmental Health and Occupational Medicine, National Health Research Institutes, 100 Shih-Chuan 1st Road, Kaohsiung 807, Taiwan (China); Tsai, E.-M. [Department of Obstetrics and Gynecology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan (China); Department of Obstetrics and Gynecology, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan (China); Tsai, F.-Y. [Division of Environmental Health and Occupational Medicine, National Health Research Institutes, 100 Shih-Chuan 1st Road, Kaohsiung 807, Taiwan (China); Chao, H.-R. [Department of Environmental and Safety Health Engineering, Chung Hwa College of Medical Technology, Tainan, Taiwan (China); Chang, Louis W. [Division of Environmental Health and Occupational Medicine, National Health Research Institutes, 100 Shih-Chuan 1st Road, Kaohsiung 807, Taiwan (China)

2005-11-15

46

Androgen Exposure Increases Human Monocyte Adhesion to Vascular Endothelium and Endothelial Cell Expression of Vascular Cell Adhesion Molecule1  

Microsoft Academic Search

Background—Male sex is an independent risk factor for coronary artery disease. Owing to the importance of monocyte adhesion to endothelial cells in the development of atherosclerosis, we hypothesized that androgens might promote this process. We therefore studied the effects of the nonaromatizable androgen dihydrotestosterone (DHT) on human monocyte adhesion to human endothelial cells and on endothelial cell-surface expression of adhesion

Jane A. McCrohon; Wendy Jessup; David J. Handelsman; David S. Celermajer

47

Very late activation antigen 4-vascular cell adhesion molecule 1 interaction is involved in the formation of erythroblastic islands  

PubMed Central

Erythroblastic islands are anatomical units consisting of a central macrophage surrounded by erythroblasts. We studied the adhesion molecules involved in the formation of these structures. Central macrophages of erythroblastic islands isolated from the spleens of phlebotomized mice were clearly stained for vascular cell adhesion molecule 1 (VCAM-1). The surrounding erythroblasts of the erythroblastic islands strongly expressed the alpha 4 integrin of very late activation antigen 4 (VLA-4: alpha 4 beta 1 integrin), the counter receptor of VCAM-1, whereas most reticulocytes and erythrocytes did not. Both monoclonal antibodies (mAbs) against alpha 4 integrin and VCAM-1 disrupted the erythroblastic islands cultured in the presence of erythropoietin. Moreover, adhesion of splenic erythroblasts to tumor necrosis factor alpha-stimulated mouse splenic endothelial cells, which showed high expression of VCAM-1 but not intercellular adhesion molecule 1, was inhibited by the anti-VCAM-1 and anti-alpha 4 mAbs. These findings suggest that VLA-4-VCAM-1 interaction plays a crucial role in the formation of erythroblastic islands. PMID:7528776

1995-01-01

48

PPARa Activators Inhibit Cytokine-Induced Vascular Cell Adhesion Molecule1 Expression in Human Endothelial Cells  

Microsoft Academic Search

Background—Adhesion molecule expression on the endothelial cell (EC) surface is critical for leukocyte recruitment to atherosclerotic lesions. Better understanding of transcriptional regulation of adhesion molecules in ECs may provide important insight into plaque formation. Peroxisome proliferator-activated receptor-a (PPARa), a member of the nuclear receptor family, regulates gene expression in response to certain fatty acids and fibric acid derivatives. The present

Nikolaus Mar; Galina K. Sukhova; Tucker Collins; Peter Libby; Jorge Plutzky

49

Human Dermal Mast Cells Contain and Release Tumor Necrosis Factor ?, which Induces Endothelial Leukocyte Adhesion Molecule 1  

NASA Astrophysics Data System (ADS)

Tumor necrosis factor ? (TNF-?) is a proinflammatory cytokine that mediates endothelial leukocyte interactions by inducing expression of adhesion molecules. In this report, we demonstrate that human dermal mast cells contain sizeable stores of immunoreactive and biologically active TNF-? within granules, which can be released rapidly into the extracellular space upon degranulation. Among normal human dermal cells, mast cells are the predominant cell type that expresses both TNF-? protein and TNF-? mRNA. Moreover, induction of endothelial leukocyte adhesion molecule 1 expression is a direct consequence of release of mast cell-derived TNF-?. These findings establish a role for human mast cells as "gatekeepers" of the dermal microvasculature and indicate that mast cell products other than vasoactive amines influence endothelium in a proinflammatory fashion.

Walsh, Laurence J.; Trinchieri, Giorgio; Waldorf, Heidi A.; Whitaker, Diana; Murphy, George F.

1991-05-01

50

Vascular cell adhesion molecule-1 (VCAM-1)-An increasing insight into its role in tumorigenicity and metastasis.  

PubMed

Vascular cell adhesion molecule-1 (VCAM-1) first attracted attention more than two decades ago as endothelial adhesion receptor with key function for leukocyte recruitment in term of cellular immune response. The early finding of VCAM-1 binding to melanoma cells, and thus a suggested mechanistic contribution to metastatic spread, was the first and for a long time the only link of VCAM-1 to cancer sciences. In the last few years, hallmarked by a growing insight into the molecular understanding of tumorigenicity and metastasis, an impressive variety of VCAM-1 functionalities in cancer have been elucidated. The present review aims to provide a current overview of VCAM-1 relevance for tumor growth, metastasis, angiogenesis, and related processes. By illustrating the intriguing role of VCAM-1 in cancer disease, VCAM-1 is suggested as a new and up to now underestimated target in cancer treatment and in clinical diagnosis of malignancies. PMID:24771582

Schlesinger, Martin; Bendas, Gerd

2015-06-01

51

Human dermal mast cells contain and release tumor necrosis factor alpha, which induces endothelial leukocyte adhesion molecule 1.  

PubMed

Tumor necrosis factor alpha (TNF-alpha) is a proinflammatory cytokine that mediates endothelial leukocyte interactions by inducing expression of adhesion molecules. In this report, we demonstrate that human dermal mast cells contain sizeable stores of immunoreactive and biologically active TNF-alpha within granules, which can be released rapidly into the extracellular space upon degranulation. Among normal human dermal cells, mast cells are the predominant cell type that expresses both TNF-alpha protein and TNF-alpha mRNA. Moreover, induction of endothelial leukocyte adhesion molecule 1 expression is a direct consequence of release of mast cell-derived TNF-alpha. These findings establish a role for human mast cells as "gatekeepers" of the dermal microvasculature and indicate that mast cell products other than vasoactive amines influence endothelium in a proinflammatory fashion. PMID:1709737

Walsh, L J; Trinchieri, G; Waldorf, H A; Whitaker, D; Murphy, G F

1991-05-15

52

Gossypol decreases tumor necrosis factor-?-induced intercellular adhesion molecule-1 expression via suppression of NF-?B activity.  

PubMed

Gossypol is a yellowish polyphenolic compound originally from cotton plant, which has been shown to exert a potential for anti-cancer and anti-inflammatory effects. However, its molecular mechanism is not thoroughly understood on breast cancer cells known to highly express intercellular adhesion molecule-1 (ICAM-1) for their adhesion and metastasis. This study aims to investigate the effect of gossypol on tumor necrosis factor (TNF)-?-stimulated ICAM-1 via nuclear factor-kappa B (NF-?B) activity. Gossypol was shown to inhibit TNF-?-induced ICAM-1 expression and U937 cell adhesion to MDA-MB-231 and MCF-7 cells. Additionally, TNF-?-induced MDA-MB-231 cell invasion was blocked in the presence of gossypol. Chromatin immunoprecipitation analysis demonstrated that gossypol blocks NF-?B binding on the ICAM-1 promoter regions. Additionally, TNF-?-induced NF-?B activation was completely suppressed in the presence of gossypol. Gossypol did not directly suppress the binding of NF-?B to the DNA but rather inhibited the nuclear translocation of p65 and p50 via phosphorylation and degradation of I?B. We also found that gossypol suppresses NF-?B activation induced by a wide variety of agents, including taxol, okadaic acid, and phorbol myristate acetate. Taken together, gossypol effectively inhibited TNF-?-induced ICAM-1 expression via the suppression of NF-?B activation and in vitro adhesion and invasion in human breast cancer cells. PMID:21223991

Moon, Dong-Oh; Choi, Yung Hyun; Moon, Sung-Kwon; Kim, Wun-Jae; Kim, Gi-Young

2011-04-01

53

Inhibition of cytokine-induced vascular cell adhesion molecule-1 expression; possible mechanism for anti-atherogenic effect of Agastache rugosa  

Microsoft Academic Search

Adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1) play an important role during the early stages of atherogenesis. Agastache rugosa has an anti-atherogenic effect in low density lipoprotein receptor ?\\/? mice. Moreover, A. rugosa reduced macrophage infiltration and VCAM-1 expression has been localized in aortic endothelium that overlies early foam cell lesions. This study ascertained that tilianin (100 ?M),

Jung-Joo Hong; Jae-Hoon Choi; Sei-Ryang Oh; Hyeong-Kyu Lee; Jae-Hak Park; Kun-Yeong Lee; Jung-Jae Kim; Tae-Sook Jeong; Goo Taeg Oh

2001-01-01

54

Epigenetic Regulation of Tumor Endothelial Cell Anergy: Silencing of Intercellular Adhesion Molecule1 by Histone Modifications  

Microsoft Academic Search

Tumors can escape from immunity by repressing leukocyte adhesion molecule expression on tumor endothelial cells and by rendering endothelial cells unresponsive to inflammatory activation. This endothelial cell anergy is induced by angiogenic growth factors and results in reduced leukocyte- vessel wall interactions, thereby attenuating infiltration of leukocytes into the tumor. This report describes a novel mechanism of endothelial cell anergy

Debby M. E. I. Hellebrekers; Karolien Castermans; Emmanuelle Vire; Nicole T. H. Hoebers; Kevin H. Mayo; Grietje Molema; Francois Fuks; Manon van Engeland; Arjan W. Griffioen

2006-01-01

55

Retinoic acid inhibits the regulated expression of vascular cell adhesion molecule-1 by cultured dermal microvascular endothelial cells.  

PubMed Central

The regulated expression of cell adhesion molecules (CAM) on endothelial cells is central to the pathogenesis of various inflammatory processes. Retinoic acid and synthetic derivatives have been demonstrated to exert antiinflammatory effects in cutaneous diseases. To determine modes of retinoid action in the modulation of inflammatory responses, we explored effects of all-trans-retinoic acid (t-RA) on the TNFalpha-induced expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin in cultured human dermal microvascular endothelial cells. Pretreatment with t-RA specifically prevented TNFalpha-induced VCAM-1 expression, but not ICAM-1 and E-selectin induction. t-RA significantly reduced VCAM-1-dependent T cell binding to TNFalpha-treated human dermal microvascular endothelial cells as well. This differential modulation of TNFalpha-induced CAM expression by t-RA was reflected at steady state mRNA levels and in nuclear run-on studies. In transcriptional activation studies, the TNFalpha-mediated activation of the human VCAM-1 promoter was inhibited after t-RA treatment, while the ICAM-1 promoter activation was unaffected, indicating that the selective inhibition of CAM expression is regulated in part at the level of gene transcription. Furthermore, the transcriptional inhibition by t-RA appears to be mediated by its effects upon the activation of NF-kappaB-dependent complex formation. Analysis of protein-DNA binding assays revealed marked inhibition of specific NF-kappaB-dependent binding to the tandem NF-KB sites of the VCAM-1 promoter, but not to the functional NF-kappaB motif of the ICAM-1 promoter. The specific inhibition of cytokine-mediated VCAM-1 gene expression in vitro may provide a potential basis by which retinoids exert their biological effects at sites of inflammation in vivo. PMID:9022083

Gille, J; Paxton, L L; Lawley, T J; Caughman, S W; Swerlick, R A

1997-01-01

56

s-ICAM-1 and s-VCAM-1 in healthy men are strongly associated with traits of the metabolic syndrome, becoming evident in the postprandial response to a lipid-rich meal  

PubMed Central

Background The importance of the postprandial state for the early stages of atherogenesis is increasingly acknowledged. We conducted assessment of association between postprandial triglycerides, insulin and glucose after ingestion of a standardized lipid-rich test meal, and soluble cellular adhesion molecules (sCAM) in young healthy subjects. Methods Metabolic parameters and sICAM-1, sVCAM-1 and E-selectin were measured before and hourly until 6 hours after ingestion of a lipid-rich meal in 30 healthy young men with fasting triglycerides <150 mg/dl and normal fasting glucose levels. Subjects were classified as either normal responders (NR) (postprandial triglyceride maxima < 260 mg/dl) or high responders (HR) (postprandial triglyceride maxima > 260 mg/dl). Levels of CAM were compared in HR and NR, and correlation with postprandial triglyceride, insulin and glucose response was assessed. Results Fasting sICAM-1 and sVCAM-1 levels were significantly higher in HR as compared to NR (p = 0.046, p = 0.03). For sE-selectin there was such a trend (p = 0.05). There was a strong positive and independent correlation between sICAM-1 and postprandial insulin maxima (r = 0.70, p < 0.001). sVCAM-1 showed significant correlation with postprandial triglycerides (AUC) (r = 0.37, p = 0.047). We found no correlation between sCAMs and fasting insulin or triglyceride concentrations. Conclusion This independent association of postprandial triglycerides with sICAM-1 may indicate a particular impact of postprandial lipid metabolism on endothelial reaction. PMID:18761738

Rubin, Diana; Claas, Sandra; Pfeuffer, Maria; Nothnagel, Michael; Foelsch, Ulrich R; Schrezenmeir, Juergen

2008-01-01

57

Benzo[a]pyrene induces intercellular adhesion molecule-1 through a caveolae and aryl hydrocarbon receptor mediated pathway  

SciTech Connect

Toxicologic and epidemiologic studies have linked benzo[a]pyrene (B[a]P) exposure with cardiovascular diseases such as atherosclerosis. The mechanisms of action leading to these diseases have not been fully understood. One key step in the development of atherosclerosis is vascular endothelial dysfunction, which is characterized by increased adhesiveness. To determine if B[a]P could lead to increased endothelial adhesiveness, the effects of B[a]P on human endothelial cell intercellular adhesion molecule-1 (ICAM-1) expression was investigated. B[a]P was able to increase ICAM-1 protein only after pretreatment with the aryl hydrocarbon receptor (AhR) agonist {beta}-naphthoflavone ({beta}-NF). Knockdown of AhR by siRNA or treatment with AhR antagonist {alpha}-naphthoflavone ({alpha}-NF) eliminated the induction of ICAM-1 from B[a]P, confirming the necessity of AhR in this process. Likewise, B[a]P only increased monocyte adhesion to the vascular endothelium when cells were pretreated with {beta}-NF. Experiments were done to define a signaling mechanism. B[a]P increased phosphorylation of MEK and p38-MAPK, and inhibitors to these proteins blunted the ICAM-1 induction. B[a]P was also able to increase AP-1 DNA binding and phosphorylation of cJun. Phosphorylation of cJun was disrupted by MEK and p38-MAPK inhibitors linking the signaling cascade. Finally, the importance of membrane microdomains, caveolae, was demonstrated by knockdown of the structural protein caveolin-1. Disruption of caveolae eliminated the B[a]P-induced ICAM-1 expression. These data suggest a possible pro-inflammatory mechanism of action of B[a]P involving caveolae, leading to increased vascular endothelial adhesiveness, and this inflammation may be a critical step in the development of B[a]P-induced atherosclerosis.

Oesterling, Elizabeth [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY 40536-0200 (United States); Toborek, Michal [Department of Neurosurgery, College of Medicine, University of Kentucky, Lexington, KY 40536-0200 (United States); Hennig, Bernhard [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY 40536-0200 (United States); Molecular and Cell Nutrition Laboratory, College of Agriculture, University of Kentucky, Lexington, KY 40536-0200 (United States)], E-mail: bhennig@uky.edu

2008-10-15

58

Platelet endothelial cell adhesion molecule-1 signaling inhibits the activation of human platelets  

Microsoft Academic Search

Platelet endothelial cell adhesion mole- cule-1 (PECAM-1\\/CD31) is a 130-kd trans- membrane glycoprotein and a member of the growing family of receptors with im- munoreceptor tyrosine-based inhibitory motifs (ITIMs). PECAM-1 is expressed on platelets, certain T cells, monocytes, neu- trophils, and vascular endothelial cells and is involved in a range of cellular processes, though the role of PECAM-1 in platelets

Milenko Cicmil; Joanne M. Thomas; Mireille Leduc; Cassian Bon; Jonathan M. Gibbins

2002-01-01

59

Epidermal Expression of Intercellular Adhesion Molecule 1 is Not a Primary Inducer of Cutaneous Inflammation in Transgenic Mice  

NASA Astrophysics Data System (ADS)

Keratinocytes at sites of cutaneous inflammation have increased expression of intercellular adhesion molecule 1 (ICAM-1), a cytokine-inducible adhesion molecule which binds the leukocyte integrins LFA-1 and Mac-1. Transgenic mice were prepared in which the expression of mouse ICAM-1 was targeted to basal keratinocytes by using the human K14 keratin promoter. The level of constitutive expression attained in the transgenic mice exceeded the peak level of ICAM-1 expression induced on nontransgenic mouse keratinocytes in vitro by optimal combinations of interferon ? and tumor necrosis factor ? or in vivo by proinflammatory stimuli such as phorbol 12-myristate 13-acetate. In vitro adhesion assays demonstrated that cultured transgenic keratinocytes were superior to normal keratinocytes as a substrate for the LFA-1-dependent binding of mouse T cells, confirming that the transgene-encoded ICAM-1 was expressed in a functional form. However, the high level of constitutive ICAM-1 expression achieved on keratinocytes in vivo in these transgenic mice did not result in additional recruitment of CD45^+ leukocytes into transgenic epidermis, nor did it elicit dermal inflammation. Keratinocyte ICAM-1 expression also did not potentiate contact-hypersensitivity reactions to epicutaneous application of haptens. The absence of a spontaneous phenotype in these transgenic mice was not the result of increased levels of soluble ICAM-1, since serum levels of soluble ICAM-1 were equal in transgenic mice and controls. We conclude that elevated ICAM-1 expression on keratinocytes cannot act independently to influence leukocyte trafficking and elicit cutaneous inflammation.

Williams, Ifor R.; Kupper, Thomas S.

1994-10-01

60

The CCL2/CCR2 Axis Enhances Vascular Cell Adhesion Molecule-1 Expression in Human Synovial Fibroblasts  

PubMed Central

Background Chemokine ligand 2 (CCL2), also known as monocyte chemoattractant protein-1 (MCP-1), belongs to the CC chemokine family that is associated with the disease status and outcomes of osteoarthritis (OA). Here, we investigated the intracellular signaling pathways involved in CCL2-induced vascular cell adhesion molecule-1 (VCAM-1) expression in human OA synovial fibroblasts (OASFs). Methodology/Principal Findings Stimulation of OASFs with CCL2 induced VCAM-1 expression. CCL2-mediated VCAM-1 expression was attenuated by CCR2 inhibitor (RS102895), PKC? inhibitor (rottlerin), p38MAPK inhibitor (SB203580), and AP-1 inhibitors (curcumin and tanshinone IIA). Stimulation of cells with CCL2 increased PKC? and p38MAPK activation. Treatment of OASFs with CCL2 also increased the c-Jun phosphorylation and c-Jun binding to the AP-1 element on the VCAM-1 promoter. Moreover, CCL2-mediated CCR2, PKC?, p38MAPK, and AP-1 pathway promoted the adhesion of monocytes to the OASFs monolayer. Conclusions/Significance Our results suggest that CCL2 increases VCAM-1 expression in human OASFs via the CCR2, PKC?, p38MAPK, c-Jun, and AP-1 signaling pathway. The CCL2-induced VCAM-1 expression promoted monocytes adhesion to human OASFs. PMID:23185512

Lin, Yu-Min; Hsu, Chin-Jung; Liao, Yuan-Ya; Chou, Ming-Chih; Tang, Chih-Hsin

2012-01-01

61

A novel function for a glucose analog of blood group H antigen as a mediator of leukocyte-endothelial adhesion via intracellular adhesion molecule 1.  

PubMed

The 4A11 antigen is a unique cytokine-inducible antigen up-regulated on rheumatoid arthritis synovial endothelium compared with normal endothelium. In soluble form, this antigen, Lewisy-6/H-5-2 (Ley/H), or its glucose analog, 2-fucosyllactose (H-2g), mediates angiogenesis. The Ley/H antigen is structurally related to the soluble E-selectin ligand, sialyl Lewisx, and is selectively expressed in skin, lymphoid organs, thymus, and synovium, suggesting that it may be important in leukocyte homing or adhesion. In the present study, we used H-2g as a functional substitute to demonstrate a novel property for Ley/H antigen in inducing leukocyte-endothelial adhesion. H-2g significantly enhanced the expression of human dermal microvascular endothelial cells (HMVECs) intercellular adhesion molecule-1 (ICAM-1), but not vascular cell adhesion molecule-1, E-selectin, and P-selectin. Immunoprecipitation and Western blotting showed glycolipids Ley-6, H-5-2, or the glucose analog H-2g quickly activated human microvascular endothelial cell line-1 (HMEC-1) Janus kinase 2 (JAK2) and that the JAK2 inhibitor, AG-490, completely inhibited HMVEC ICAM-1 expression and HL-60 adhesion to HMEC-1s. Use of a JAK/signal transducer and activator of transcription (STAT) profiling system confirmed that H-2g selectively activated STAT3 but not STAT1 and STAT2. AG-490 inhibited H-2g-induced Erk1/2 and PI3K-Akt activation, suggesting that JAK2 is upstream of the Erk1/2 and PI3K-Akt pathways. Furthermore, the JAK2 inhibitor AG-490, the Erk1/2 inhibitor PD98059, or the phosphatidylinositol 3-kinase inhibitor LY294002 or antisense oligodeoxynucleotides directed against JAK2, Erk1/2, or phosphatidylinositol 3-kinase blocked H-2g-induced HMVEC ICAM-1 expression and HL-60 adhesion to HMEC-1s. Hence, H-2g signals through JAK2 and its downstream signal transducers STAT3, Erk1/2, and phosphatidylinositol 3-kinase result in ICAM-1 expression and cell adhesion. Potential treatment strategies through the inhibition of JAK-dependent pathways to target H-2g signals may provide a useful approach in inflammation-driven diseases like rheumatoid arthritis. PMID:12672794

Zhu, Kui; Amin, M Asif; Kim, Michael J; Katschke, Kenneth J; Park, Christy C; Koch, Alisa E

2003-06-13

62

Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental rabbits fed with cholesterol diet  

PubMed Central

Background Inflammation process plays an important role in the development of atherosclerosis. Hypercholesterolemia is one of the major risk factors for atherosclerosis. The present study aimed to evaluate the effect of aqueous extract of Piper sarmentosum (P.s) on inflammatory markers like vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and C-reactive protein (CRP). Methods Forty two male New Zealand white rabbits were divided equally into seven groups; (i) C- control group fed normal rabbit chow (ii) CH- cholesterol diet (1%cholesterol) (iii) X1- 1% cholesterol with water extract of P.s (62.5 mg/kg) (iv) X2- 1% cholesterol with water extract of P.s (125 mg/kg (v) X3- 1% cholesterol with water extract of P.s (250 mg/kg) (vi) X4- 1% cholesterol with water extract of P.s (500 mg/kg) and (vii) SMV group fed with 1% cholesterol supplemented with simvistatin drug (1.2 mg/kg). All animals were treated for 10 weeks. Blood serum was taken for observing the inflammatory markers at the beginning and end of the experiment. Results Rabbits fed with 1% cholesterol diet (CH) showed significant increase in the level of VCAM-1, ICAM-1 and CRP compared to the C group. The levels of VCAM-1, ICAM-1 and CRP in the 1% cholesterol group and supplemented with P.s (500 mg/kg) were significantly reduced compared to the cholesterol group. Similar results were also reported with simvistatin group. Conclusion These results suggest that the supplementation of Piper sarmentosum extract could inhibit inflammatory markers which in turn could prevent atherosclerosis. PMID:21214952

2011-01-01

63

Organization, regulatory sequences, and alternatively spliced transcripts of the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) gene  

SciTech Connect

The mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is expressed selectively at venular sites of lymphocyte extravasation into mucosal lymphoid tissues and lamina propria, where it directs local lymphocyte trafficking. MAdCAM-1 is a multifunctional type I transmembrane adhesion molecule comprising two distal Ig domains involved in {alpha}4{beta}7 integrin binding, a mucin-like region able to display L-selectin-binding carbohydrates, and a membrane-proximal Ig domain homologous to IgA. We show in this work that the MAdCAM-1 gene is located on chromosome 10 and contains five exons. The signal peptide and each one of the three Ig domains are encoded by a distinct exon, whereas the transmembrane, cytoplasmic tail, and 3{prime}-untranslated region of MAdCAM-1 are combined on a single exon. The mucin-like region and the third Ig domain are encoded together on exon 4. An alternatively spliced MAdCAM-1 mRNA is identified that lacks the mucin/IgA-homologous exon 4-encoded sequences. This short variant of MAdCAM-1 may be specialized to support {alpha}4{beta}7-dependent adhesion strengthening, independent of carbohydrate-presenting function. Sequences 5{prime} of the transcription start site include tandem nuclear factor-KB sites; AP-1, AP-2, and signal peptide-1 binding sites; and an estrogen response element. Our findings reinforce the correspondence between the multidomain structure and versatile functions of this vascular addressin, and suggest an additional level of regulation of carbohydrate-presenting capability, and thus of its importance in lectin-mediated vs. {alpha}4{beta}7-dependent adhesive events in lymphocyte trafficking. 46 refs., 6 figs., 1 tab.

Sampaio, S.O.; Mei, C.; Butcher, E.C. [Stanford Univ. School of Medicine, CA (United States)] [and others

1995-09-01

64

Enhanced expression of intracellular adhesion molecule-1 and P-selectin in the diabetic human retina and choroid.  

PubMed Central

Elevated expression of intercellular adhesion molecule-1 (ICAM-1) as well as E- and P-selectin occurs on the vascular endothelium in a number of disease states and is thought to play an early critical role in the adhesion of circulating leukocytes to the endothelium. The goal of the present study was to investigate the immunolocalization of these molecules in the retina and choroid of postmortem human tissue sections from nondiabetic and diabetic subjects. Whereas ICAM-1 was localized primarily within the choriocapillaris of nondiabetic subjects, immunoreactivity in diabetics was significantly elevated throughout the choroidal vasculature and within retinal blood vessels (P < 0.05). In the choroid, P-selectin was most prominent in veins of the nondiabetic, whereas in diabetics, P-selectin was significantly elevated in arteries (P < 0.001) and veins (P < 0.05) and, in some cases, was also observed in choriocapillaris. P-selectin immunoreactivity was not observed in the retina of any subject. E-selectin immunoreactivity was not observed in choroid or retina in any subjects. Neutrophil numbers per square millimeter of tissue were significantly elevated in diabetic choroid (P < 0.05) and retina (P < 0.001). Our results demonstrate that ICAM-1 and P-selectin are constitutively expressed in the normal choroid and are upregulated in the choroidal vasculature in diabetes, but only ICAM-1 was upregulated in the retina of diabetic subjects. Increased cell adhesion molecule expression may contribute to the retinal and choroidal microangiopathy observed in diabetics by enhancing leukocyte adhesion and consequently the incidence of capillary obstruction and endothelial cell injury. Images Figure 1 Figure 2 Figure 3 Figure 4 PMID:7545873

McLeod, D. S.; Lefer, D. J.; Merges, C.; Lutty, G. A.

1995-01-01

65

Platelet Endothelial Cell Adhesion Molecule?1 Mediates Endothelial?Cardiomyocyte Communication and Regulates Cardiac Function  

PubMed Central

Background Dilated cardiomyopathy is characterized by impaired contractility of cardiomyocytes, ventricular chamber dilatation, and systolic dysfunction. Although mutations in genes expressed in the cardiomyocyte are the best described causes of reduced contractility, the importance of endothelial?cardiomyocyte communication for proper cardiac function is increasingly appreciated. In the present study, we investigate the role of the endothelial adhesion molecule platelet endothelial cell adhesion molecule (PECAM?1) in the regulation of cardiac function. Methods and Results Using cell culture and animal models, we show that PECAM?1 expressed in endothelial cells (ECs) regulates cardiomyocyte contractility and cardiac function via the neuregulin?ErbB signaling pathway. Conscious echocardiography revealed left ventricular (LV) chamber dilation and systolic dysfunction in PECAM?1?/? mice in the absence of histological abnormalities or defects in cardiac capillary density. Despite deficits in global cardiac function, cardiomyocytes isolated from PECAM?1?/? hearts displayed normal baseline and isoproterenol?stimulated contractility. Mechanistically, absence of PECAM?1 resulted in elevated NO/ROS signaling and NRG?1 release from ECs, which resulted in augmented phosphorylation of its receptor ErbB2. Treatment of cardiomyocytes with conditioned media from PECAM?1?/? ECs resulted in enhanced ErbB2 activation, which was normalized by pre?treatment with an NRG?1 blocking antibody. To determine whether normalization of increased NRG?1 levels could correct cardiac function, PECAM?1?/? mice were treated with the NRG?1 blocking antibody. Echocardiography showed that treatment significantly improved cardiac function of PECAM?1?/? mice, as revealed by increased ejection fraction and fractional shortening. Conclusions We identify a novel role for PECAM?1 in regulating cardiac function via a paracrine NRG1?ErbB pathway. These data highlight the importance of tightly regulated cellular communication for proper cardiac function. PMID:25600142

McCormick, Margaret E.; Collins, Caitlin; Makarewich, Catherine A.; Chen, Zhongming; Rojas, Mauricio; Willis, Monte S.; Houser, Steven R.; Tzima, Ellie

2015-01-01

66

Regulatory elements and transcription factors controlling basal and cytokine-induced expression of the gene encoding intercellular adhesion molecule 1.  

PubMed

The gene encoding intercellular adhesion molecule 1 (ICAM-1) is transcriptionally induced in response to inflammatory and immunomodulatory cytokines. To investigate the mechanisms controlling ICAM-1 gene expression, we have identified regulatory DNA sequences responsible for maintaining basal and mediating induced transcription in response to tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma). Regulatory elements centered 115, 60, and 40 bp upstream from the ICAM-1 transcription start site were implicated in cytokine-independent gene expression. Regulatory elements dedicated to TNF-alpha and IFN-gamma were identified 190 and 90 bp, respectively, upstream from the ICAM-1 transcription start site. A combination of mutagenesis and DNA-binding assays revealed that the TNF-alpha response element is composite, consisting of binding sites for both C/EBP and NF-kappa B. The IFN-gamma response element behaved as a simple regulatory element that selectively binds to an IFN-gamma-inducible activity composed, at least in part, of p91. These observations provide a framework for understanding how extracellular signals dynamically regulate the adhesive properties of mammalian cells. PMID:7972116

Hou, J; Baichwal, V; Cao, Z

1994-11-22

67

Requirement for Intercellular Adhesion Molecule 1 and Caveolae in Invasion of Human Oral Epithelial Cells by Porphyromonas gingivalis  

PubMed Central

Porphyromonas gingivalis, a periodontopathic bacterium, is known to invade oral epithelial cells in periodontal lesions, although the mechanism is unclear. In the present study, goat polyclonal anti-intercellular adhesion molecule 1 (anti-ICAM-1) antibody inhibited the invasion of P. gingivalis into KB cells (human oral epithelial cells). Further, the P. gingivalis fimbria, a pathogenic adhesion molecule, bound to recombinant human ICAM-1, as shown by enzyme-linked immunosorbent assay. P. gingivalis was also found to colocalize with ICAM-1 on KB cells, as seen with an immunofluorescence microscope, and the knockdown of ICAM-1 in KB cells resulted in the inhibition of P. gingivalis invasion by RNA interference. In addition, methyl-?-cyclodextrin, a cholesterol-binding agent, inhibited the colocalization of P. gingivalis with ICAM-1 and invasion by the microorganism. The colocalization of caveolin-1, a caveolar marker protein, on KB cells with P. gingivalis was also shown, and the knockdown of caveolin-1 in KB cells caused a reduced level of P. gingivalis invasion. These results suggest that ICAM-1 and caveolae are required for the invasion of P. gingivalis into human oral epithelial cells, and these molecules appear to be associated with the primary stages of the development and progression of chronic periodontitis. PMID:16177300

Tamai, Riyoko; Asai, Yasuyuki; Ogawa, Tomohiko

2005-01-01

68

A randomized, double-blind trial of the effect of treatment with montelukast on bronchial hyperresponsiveness and serum eosinophilic cationic protein (ECP), soluble interleukin 2 receptor (sIL2R), IL4, and soluble intercellular adhesion molecule 1 (sICAM-1) in children with asthma  

Microsoft Academic Search

Background: Anti-inflammatory properties of leukotriene modifiers and their effect on bronchial hyperresponsiveness have not been studied in children with asthma. Objective: The primary objective of this study was to determine the changes in serum levels of inflammatory mediators, clinical efficacy, and bronchial hyperresponsiveness after treatment with montelukast. Methods: In this double-blind, randomized, placebo-controlled trial, 39 children with mild-to-moderate atopic asthma

Iwona Stelmach; Joanna Jerzynska; Piotr Kuna

2002-01-01

69

Association between the Polymorphisms in Intercellular Adhesion Molecule-1 and the Risk of Coronary Atherosclerosis: A Case-Controlled Study  

PubMed Central

Intercellular adhesion molecule-1 (ICAM-1), an important immune adhesion molecule, is related to the atherosclerosis. We explored the association between the polymorphisms of the ICAM-1 gene and coronary atherosclerotic stenosis to determine whether any risk factors correlate with genetic polymorphisms in Chinese patients with coronary atherosclerosis. Using the SNaPshot assay, we examined six SNPs of rs5491, rs281428, rs281432, rs5496, rs5498 and rs281437 in 604 patients diagnosed with coronary atherosclerotic stenosis by angiography and in 468 controls. We found that AG genotype of rs5498 had higher frequency in the coronary atherosclerotic stenosis patients (41.56% to 34.19%, P?=?0.017, OR?=?1.368,95%CI 1.057–1.770) and that the haplotype Ars5491Crs281428Grs281432 had higher frequency in patients (13.8% to 12.1%, P?=?0.048). When analyzing the clinical risk factors for coronary atherosclerosis, we found that the rs5498 locus was associated with the levels of apolipoprotein A (APOA) (P?=?0.0002) and triglycerides (TG) (P?=?0.002). Furthermore, the levels of triglycerides (TG) were also associated with rs281432 (P?=?0.040). Additionally, the TT genotype of rs281437 was associated with a higher level of apolipoprotein A (APOA) (P?=?0.039) and apolipoprotein B (APOB) (P?=?0.003). Finally, among those with coronary atherosclerosis, we found no differences in the haplotype analysis of polymorphisms of the ICAM-1 gene from individuals with hypertension or those who smoked. According to our results, the ICAM-1 polymorphisms were associated with risk of coronary atherosclerotic stenosis in Chinese individuals. PMID:25310099

Zhang, Qingjiang; Xin, Yu; Chen, Yanjun; Tian, Ye

2014-01-01

70

Expression of a Soluble Isoform of Cell Adhesion Molecule 1 in the Brain and Its Involvement in Directional Neurite Outgrowth  

PubMed Central

Cell adhesion molecule 1 (CADM1), an immunoglobulin superfamily member, is expressed on superior cervical ganglion neurites and mediates cell–cell adhesion by trans-homophilic binding. In addition to the membrane-bound form, we have previously shown that a soluble form (sCADM1) generated by alternative splicing possesses a stop codon immediately downstream of the immunoglobulin-like domain. Here, we demonstrate the presence of sCADM1 in vivo and its possible role in neurite extension. sCADM1 appears to be a stromal protein because extracellular-restricted, but not intracellular-restricted, anti-CADM1 antibody stained stromal protein-rich extract from mouse brains. Murine plasmacytoma cells, P3U1, were modified to secrete sCADM1 fused with either immunoglobulin (Ig)G Fc portion (sCADM1-Fc) or its deletion form that lacks the immunoglobulin-like domain (?sCADM1-Fc). When P3U1 derivatives expressing sCADM1-Fc or ?sCADM1-Fc were implanted into collagen gels, Fc-fused proteins were present more abundantly around the cells. Superior cervical ganglion neurons, parental P3U1, and either derivative were implanted into collagen gels separately, and co-cultured for 4 days. Bodian staining of the gel sections revealed that most superior cervical ganglion neurites turned toward the source of sCADM1-Fc, but not ?sCADM1-Fc. Furthermore, immunofluorescence signals for sCADM1-Fc and membrane-bound CADM1 were co-localized on the neurite surface. These results show that sCADM1 appears to be involved in directional neurite extension by serving as an anchor to which membrane-bound CADM1 on the neurites can bind. PMID:19435791

Hagiyama, Man; Ichiyanagi, Naoki; Kimura, Keiko B.; Murakami, Yoshinori; Ito, Akihiko

2009-01-01

71

Wiskott-Aldrich syndrome protein controls antigen-presenting cell-driven CD4+ T-cell motility by regulating adhesion to intercellular adhesion molecule-1.  

PubMed

T-cell scanning for antigen-presenting cells (APC) is a finely tuned process. Whereas non-cognate APC trigger T-cell motility via chemokines and intercellular adhesion molecule-1 (ICAM-1), cognate APC deliver a stop signal resulting from antigen recognition. We tested in vitro the contribution of the actin cytoskeleton regulator Wiskott-Aldrich syndrome protein (WASP) to the scanning activity of primary human CD4(+)  T cells. WASP knock-down resulted in increased T-cell motility upon encounter with non-cognate dendritic cells or B cells and reduced capacity to stop following antigen recognition. The high motility of WASP-deficient T cells was accompanied by a diminished ability to round up and to stabilize pauses. WASP-deficient T cells migrated in a normal proportion towards CXCL12, CCL19 and CCL21, but displayed an increased adhesion and elongation on ICAM-1. The elongated morphology of WASP-deficient T cells was related to a reduced confinement of high-affinity lymphocyte function-associated antigen 1 to the mid-cell zone. Our data therefore indicate that WASP controls CD4(+) T-cell motility upon APC encounter by regulating lymphocyte function-associated antigen 1 spatial distribution. PMID:22804504

Lafouresse, Fanny; Cotta-de-Almeida, Vinicius; Malet-Engra, Gema; Galy, Anne; Valitutti, Salvatore; Dupré, Loïc

2012-10-01

72

Wiskott–Aldrich syndrome protein controls antigen-presenting cell-driven CD4+ T-cell motility by regulating adhesion to intercellular adhesion molecule-1  

PubMed Central

T-cell scanning for antigen-presenting cells (APC) is a finely tuned process. Whereas non-cognate APC trigger T-cell motility via chemokines and intercellular adhesion molecule-1 (ICAM-1), cognate APC deliver a stop signal resulting from antigen recognition. We tested in vitro the contribution of the actin cytoskeleton regulator Wiskott–Aldrich syndrome protein (WASP) to the scanning activity of primary human CD4+ T cells. WASP knock-down resulted in increased T-cell motility upon encounter with non-cognate dendritic cells or B cells and reduced capacity to stop following antigen recognition. The high motility of WASP-deficient T cells was accompanied by a diminished ability to round up and to stabilize pauses. WASP-deficient T cells migrated in a normal proportion towards CXCL12, CCL19 and CCL21, but displayed an increased adhesion and elongation on ICAM-1. The elongated morphology of WASP-deficient T cells was related to a reduced confinement of high-affinity lymphocyte function-associated antigen 1 to the mid-cell zone. Our data therefore indicate that WASP controls CD4+ T-cell motility upon APC encounter by regulating lymphocyte function-associated antigen 1 spatial distribution. PMID:22804504

Lafouresse, Fanny; Cotta-de-Almeida, Vinicius; Malet-Engra, Gema; Galy, Anne; Valitutti, Salvatore; Dupré, Loïc

2012-01-01

73

Involvement of Interferon-Gamma Genetic Variants and Intercellular Adhesion Molecule-1 in Onset and Progression of Generalized Vitiligo  

PubMed Central

Interferon-gamma (IFN-?) is a paracrine inhibitor of melanocytes and genetic variability due to intron 1 polymorphisms in IFNG has been reported to be associated with increased risk for several autoimmune diseases. The aim of present study was to determine whether intron 1 +874A/T (rs2430561) and CA microsatellite (rs3138557) polymorphisms in IFNG are associated with generalized vitiligo (GV) susceptibility and expression of IFNG and intercellular adhesion molecule-1 (ICAM1) affects the disease onset and progression. Here we report that IFNG CA microsatellite but not +874A/T may be a genetic risk factor for GV; however, +874T allele plays a crucial role in increased expression of IFNG mRNA and protein levels which could affect the onset and progression of the disease. Active GV patients showed increased IFNG levels compared to stable GV patients. The genotype-phenotype analysis revealed that IFNG expression levels were higher in patients with +874 TT genotypes and 12 CA repeats. Patients with the early age of onset showed higher IFNG expression and female GV patients showed higher IFNG and ICAM1 expression implicating gender biasness and involvement of IFN-? in early onset of the disease. Moreover, the increased IFN-? levels in patients lead to increased ICAM1 expression, which could be a probable link between cytokines and T-cell involvement in pathogenesis of GV. PMID:23777204

Dwivedi, Mitesh; Laddha, Naresh C.; Shah, Kriti; Shah, Bela J.

2013-01-01

74

Effects of a thrombomodulin-derived peptide on monocyte adhesion and intercellular adhesion molecule-1 expression in lipopolysaccharide-induced endothelial cells  

PubMed Central

Purpose It has been documented that GC31, a 31-animo acid peptide from human thrombomodulin, has potent anti-inflammatory properties in endotoxin-induced uveitis and lipopolysaccharide (LPS)-induced RAW264.7 cells, while the role of GC31 in the endothelial cells has not yet been fully understood. Therefore, the aim of this study was to explore the effect of GC31 on intercellular adhesion molecule-1 (ICAM-1) expression in LPS-activated endothelial cells. Methods Human umbilical vein endothelial cells (HUVECs) were incubated with LPS (1 ?g/ml) and peptide GC31 or control peptide VP30 simultaneously. ICAM-1 messenger RNA and protein levels were evaluated with real-time PCR and western blot. The adhesion of U937 cells labeled with CM-H2DCFDA to HUVECs was examined with ?uorescence microscope. Extracellular signal-regulated kinase-1/2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK) activation, inhibitor of nuclear factor kappa B alpha (I?B?) degradation, and nuclear factor kappa B (NF-?B) nuclear translocation were detected with western blot. Results Upon LPS stimulation, GC31 suppressed the mRNA and protein expression of ICAM-1 in HUVECs and remarkably reduced monocyte-endothelial cell adhesion in a dose-dependent manner. Furthermore, GC31 significantly inhibited the degradation of I?B? and nuclear translocation of NF-?B and moderately blocked the activation of p38 MAPK and ERK1/2 in activated HUVECs. Conclusions Our results suggested that GC31 suppressed LPS-mediated ICAM-1 expression by inhibiting the activation of NF-?B and partially by attenuating the activity of ERK1/2 and p38 MAPK in vascular endothelium, which may contribute to ameliorating vascular inflammatory diseases, such as uveitis. PMID:23401649

Xu, Yan; Xu, Xun; Yang, Xiaolu; Gu, Qing; Liu, Kun

2013-01-01

75

Immunologic changes in TNF-alpha, sE-selectin, sP-selectin, sICAM-1, and IL-8 in pediatric patients treated for psoriasis with the Goeckerman regimen  

SciTech Connect

Psoriasis is a chronic inflammatory skin disease which is often manifested during childhood. The present study investigated changes in the serum levels of proinflammatory cytokines and soluble forms of adhesion molecules in children with psoriasis. The observed patient group of 26 children was treated with the Goeckerman regimen. This therapy combines dermal application of crude coal tar with ultraviolet radiation. The Psoriasis Area Severity Index decreased significantly after treatment by with the Goeckerman regimen (p < 0.001). Serum levels of the proinflammatory cytokine TNF-alpha and adhesion molecules sICAM-1, sP-selectin and sE-selectin decreased after the Goeckerman regimen. The TNF-alpha and sICAM-1 decreased significantly (p < 0.05). Our findings support the complex role of these immune parameters in the immunopathogenesis of psoriasis in children. The serum level of IL-8 increased after the Goeckerman regimen. This fact indicates that the chemokine pathway of IL-8 activity could be modulated by this treatment, most likely by polycyclic aromatic hydrocarbons.

Borska, L.; Fiala, Z.; Krejsek, J.; Andrys, C.; Vokurkova, D.; Hamakova, K.; Kremlacek, J.; Ettler, K. [Charles University of Prague, Hradec Kralove (Czech Republic). Faculty of Medicine

2007-11-15

76

Intercellular Adhesion Molecule-1 K469E Polymorphism and Risk of Coronary Artery Disease: A Meta-Analysis  

PubMed Central

Background Intercellular adhesion molecule-1 (ICAM-1) K469E polymorphism has been implicated in susceptibility to coronary artery disease (CAD). Several studies investigated the association of this polymorphism with CAD in different populations but the results were contradictory. A meta-analysis was conducted to assess the association between ICAM-1 K469E polymorphism and CAD susceptibility. Material/Methods Databases including PubMed, EMBASE, China National Knowledge Infrastructure (CNKI), and Weipu Database were searched to find relevant studies. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of associations. A random-effects model was used. Results Fifteen case-control studies including 3088 cases and 3466 controls were included. Overall, a significant association between ICAM-1 K469E polymorphism and CAD was observed in the dominant model (OR=1.80; 95% CI 1.62–2.01; P<0.00001; Pheterogeneity=0.40). In subgroup analysis by ethnicity, a significant association was found among Asians (OR=1.92; 95% CI 1.51–2.43; P<0.00001; Pheterogeneity=0.98) and among Caucasians (OR=1.64; 95% CI 1.30–2.08; P<0.0001; Pheterogeneity=0.04). In the subgroup analysis by age, a significant association was found among young patients (OR=1.46; 95% CI 1.10–1.93; P=0.008; Pheterogeneity=0.21) and old patients (OR=1.92; 95% CI 1.75–2.10; P<0.00001; Pheterogeneity=0.99). Conclusions Results of this meta-analysis suggest that ICAM-1 K469E polymorphism confers a risk factor of CAD. PMID:25503951

Zou, Shengqiang; Pan, Xin; Chen, Zhigang; Wei, Chao; He, Bin; Zhang, Heng

2014-01-01

77

Increased soluble vascular adhesion molecule-1 concentration is associated with impaired coronary flow reserve in cardiac syndrome X.  

PubMed

It is well known that both atherosclerosis initiated by endothelial dysfunction due to inflammatory cascade and coronary flow reserve (CFR) are useful in the functional or risk assessment of coronary microcirculation. The aim of this study is to elucidate the association between early atherosclerotic inflammatory markers and CFR using transthoracic echocardiography (TTE) in subjects with cardiac syndrome X. A total of 135 individuals (mean age 56 ± 9 years, 79 males and 56 females) with angina or angina-like chest pain and a normal coronary angiogram were enrolled. The early inflammatory biomarkers related to atherosclerosis, namely soluble vascular adhesion molecule-1 (sVCAM-1), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and lipoprotein-associated phospholipase A2 (Lp-PLA2), were compared with analyzed CFR, using TTE and adenosine or dipyridamole, measured within 2 weeks after coronary angiography. There was an inverse correlation between sVCAM-1 and CFR (r = -0.225, P = 0.014). In the two groups divided by a CFR of 2.0, sVCAM-1 was significantly higher in the group with CFR <2.0 than in the group with CFR ?2.0 (n = 21: 757 ± 323 ng/ml, vs n = 114: 628 ± 146 ng/ml, P = 0.007). In multivariate analysis, sVCAM-1 was an independent factor related to a CFR <2.0 (odds ratio 1.003, 95 % confidence interval 1.001-1.006, P = 0.023). Our results showed that sVCAM-1 levels were inversely associated with CFR using TTE in cardiac syndrome X. Further studies are warranted to validate whether increased sVCAM-1 concentration, as an inflammatory modulator, is reflected in the presence of subclinical coronary atherosclerosis. PMID:24173712

Shim, Byung Ju; Lee, Dong Hyeon; Youn, Ho Joong

2014-11-01

78

Carcinoembryonic antigen-related cellular adhesion molecule 1 isoforms alternatively inhibit and costimulate human T cell function.  

PubMed

Carcinoembryonic Ag-related cellular adhesion molecule 1 (CEACAM1) represents a group of transmembrane protein isoforms that consist of variable numbers of extracellular Ig-like domains together with either a long cytoplasmic (cyt) tail containing two immunoreceptor tyrosine-based inhibitory motifs or a unique short cyt tail. Although CEACAM1 has been reported to be expressed on the surface of T lymphocytes upon activation, its roles in T cell regulation are controversial due to the lack of functional characterization of each individual CEACAM1 isoform. We thus cotransfected Jurkat T cells with CEACAM1 isoform-encoding constructs and an IL-2 promoter-bearing plasmid or a small interference RNA targeting src homology domain 2 containing phosphatase 1. In a luciferase reporter assay and through measurements of cytokine secretion (IL-2, IL-4, and IFN-gamma), CEACAM1 containing either a long or a short cyt tail inhibited or costimulated, respectively, TCR/CD3 complex plus CD28 mediated activation with the inhibitory functions of the long cyt tail dominating. The inhibitory function of CEACAM1, was dependent upon src homology domain 2 containing phosphatase 1 activity, required both tyrosine residues within the immunoreceptor tyrosine-based inhibitory motif domains of the cyt tail and was mediated through the mitogen-activated protein kinase pathway. CEACAM1-mediated inhibition could be functionally reconstituted by incubation of PBMC with either a CEACAM1-specific mAb or CEACAM1-Fc fusion protein in the presence of an allogeneic or mitogenic stimulus, respectively. These studies indicate that the long and short cyt tails of CEACAM1 serve as inhibitory and costimulatory receptors, respectively, in T cell regulation. PMID:15004154

Chen, Daohong; Iijima, Hideki; Nagaishi, Takashi; Nakajima, Atsushi; Russell, Sara; Raychowdhury, Raktima; Morales, Victor; Rudd, Christopher E; Utku, Nalan; Blumberg, Richard S

2004-03-15

79

An anti-platelet-endothelial cell adhesion molecule-1 antibody inhibits leukocyte extravasation from mesenteric microvessels in vivo by blocking the passage through the basement membrane  

Microsoft Academic Search

Summary Platelet-endothelial cell adhesion molecule-1 (PECAM-1, CD31) plays an active role in the process of leukocyte migration through cultured endothelial cells in vitro and anti-PECAM-1 antibodies (Abs) inhibit accumulation ofleukocytes into sites of inflammation in vivo. Despite the latter, it is still not clear at which stage of leukocyte emigration in vivo PECAM-1 is in- volved. To address this point

M. W. Wakelin; M.-J. Sanz; A. Dewar; S. M. Albelda; S. W. Larkin; N. Boughton-Smith; T. J. Williams; S. Nourshargh

1996-01-01

80

Elevated serum levels of soluble adhesion molecules predict death in pre- dialysis patients: association with malnutrition, inflammation, and cardiovascular disease  

Microsoft Academic Search

regression model showed that elevated sICAM-1 was, independent of age, SGA, CVD, and Log CRP, genesis via effects on soluble adhesion molecules or if elevated serum levels of soluble adhesion molecules are Methods. Serum levels of sVCAM-1, sICAM-1 (n= 87) and sE-selectin (n=71) were analysed in a cohort merely markers of endothelial activation in patients with chronic renal failure. of

Peter Stenvinkel; Bengt Lindholm; Mikael Heimburger; Olof Heimburger

81

NOX2 in lung inflammation: quantum dot based in situ imaging of NOX2-mediated expression of vascular cell adhesion molecule-1  

PubMed Central

Quantum dot (QD) imaging is a powerful tool for studying signaling pathways as they occur. Here we employ this tool to study adhesion molecule expression with lung inflammation in vivo. A key event in pulmonary inflammation is the regulation of vascular endothelial cell adhesion molecule-1 (VCAM), which drives activated immune cell adherence. The induction of VCAM expression is known to be associated with reactive oxygen species (ROS) production, but the exact mechanism or the cellular source of ROS that regulates VCAM in inflamed lungs is not known. NADPH oxidase 2 (NOX2) has been reported to be a major source of ROS with pulmonary inflammation. NOX2 is expressed by both endothelial and immune cells. Here we use VCAM-targeted QDs in a mouse model to show that NOX2, specifically endothelial NOX2, induces VCAM expression with lung inflammation in vivo. PMID:24318114

Orndorff, Rebecca L.; Hong, Nankang; Yu, Kevin; Feinstein, Sheldon I.; Zern, Blaine J.; Fisher, Aron B.; Muzykantov, Vladimir R.

2013-01-01

82

Inhibition of cytokine-induced vascular cell adhesion molecule-1 expression; possible mechanism for anti-atherogenic effect of Agastache rugosa.  

PubMed

Adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1) play an important role during the early stages of atherogenesis. Agastache rugosa has an anti-atherogenic effect in low density lipoprotein receptor -/- mice. Moreover, A. rugosa reduced macrophage infiltration and VCAM-1 expression has been localized in aortic endothelium that overlies early foam cell lesions. This study ascertained that tilianin (100 microM), a major component of A. rugosa, inhibits the tumor necrotic factor-alpha (TNF-alpha)-induced expression of VCAM-1 by 74% in cultured human umbilical vein endothelial cells (HUVECs). Also, tilianin (100 microM) reduced TNF-alpha-induced activation of nuclear factor-kappaB in HUVECs. PMID:11334881

Hong, J J; Choi, J H; Oh, S R; Lee, H K; Park, J H; Lee, K Y; Kim, J J; Jeong, T S; Oh, G T

2001-04-27

83

Suppression of complement regulatory protein C1 inhibitor in vascular endothelial activation by inhibiting vascular cell adhesion molecule-1 action  

SciTech Connect

Increased expression of adhesion molecules by activated endothelium is a critical feature of vascular inflammation associated with the several diseases such as endotoxin shock and sepsis/septic shock. Our data demonstrated complement regulatory protein C1 inhibitor (C1INH) prevents endothelial cell injury. We hypothesized that C1INH has the ability of an anti-endothelial activation associated with suppression of expression of adhesion molecule(s). C1INH blocked leukocyte adhesion to endothelial cell monolayer in both static assay and flow conditions. In inflammatory condition, C1INH reduced vascular cell adhesion molecule (VCAM-1) expression associated with its cytoplasmic mRNA destabilization and nuclear transcription level. Studies exploring the underlying mechanism of C1INH-mediated suppression in VCAM-1 expression were related to reduction of NF-{kappa}B activation and nuclear translocation in an I{kappa}B{alpha}-dependent manner. The inhibitory effects were associated with reduction of inhibitor I{kappa}B kinase activity and stabilization of the NF-{kappa}B inhibitor I{kappa}B. These findings indicate a novel role for C1INH in inhibition of vascular endothelial activation. These observations could provide the basis for new therapeutic application of C1INH to target inflammatory processes in different pathologic situations.

Zhang, Haimou [Center for Infection and Immunity Research, School of Life Sciences, Hubei University, Wuhan, Hubei (China); Qin, Gangjian [Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, Chicago, IL (United States); Liang, Gang [Children's Hospital, Harvard Medical School, Boston, MA (United States); Li, Jinan [CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA (United States); Chiu, Isaac [CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA (United States); Barrington, Robert A. [CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA (United States); Liu, Dongxu [Center for Infection and Immunity Research, School of Life Sciences, Hubei University, Wuhan, Hubei (China)]. E-mail: dxliu001@yahoo.com

2007-07-13

84

Human Dermal Mast Cells Contain and Release Tumor Necrosis Factor alpha, which Induces Endothelial Leukocyte Adhesion Molecule 1  

Microsoft Academic Search

Tumor necrosis factor alpha (TNF-alpha) is a proinflammatory cytokine that mediates endothelial leukocyte interactions by inducing expression of adhesion molecules. In this report, we demonstrate that human dermal mast cells contain sizeable stores of immunoreactive and biologically active TNF-alpha within granules, which can be released rapidly into the extracellular space upon degranulation. Among normal human dermal cells, mast cells are

Laurence J. Walsh; Giorgio Trinchieri; Heidi A. Waldorf; Diana Whitaker; George F. Murphy

1991-01-01

85

Feverfew Extracts and the Sesquiterpene Lactone Parthenolide Inhibit Intercellular Adhesion Molecule1 Expression in Human Synovial Fibroblasts  

Microsoft Academic Search

Previous studies have shown that extracts of the aromatic herb feverfew (Tanacetum parthenium) and one of its bioactive components, parthenolide, have anti-inflammatory properties in vivo and in vitro. We examined both crude feverfew extracts and purified parthenolide for their ability to modulate adhesion molecule expression in human synovial fibroblasts. Pretreatment of synovial fibroblasts with either feverfew extracts or purified parthenolide

Theresa H. Piela-Smith; Xiufang Liu

2001-01-01

86

Sesamin attenuates intercellular cell adhesion molecule-1 expression in vitro in TNF-alpha-treated human aortic endothelial cells and in vivo in apolipoprotein-E-deficient mice.  

PubMed

Sesame lignans have antioxidative and anti-inflammatory properties. We focused on the effects of the lignans sesamin and sesamol on the expression of endothelial-leukocyte adhesion molecules in tumor necrosis factor-alpha (TNF-alpha)-treated human aortic endothelial cells (HAECs). When HAECs were pretreated with sesamin (10 or 100 microM), the TNF-alpha-induced expression of intercellular cell adhesion molecule-1 (ICAM-1) was significantly reduced (35 or 70% decrease, respectively) by Western blotting. Sesamol was less effective at inhibiting ICAM-1 expression (30% decrease at 100 microM). Sesamin and sesamol reduced the marked TNF-alpha-induced increase in human antigen R (HuR) translocation and the interaction between HuR and the 3'UTR of ICAM-1 mRNA. Both significantly reduced the binding of monocytes to TNF-alpha-stimulated HAECs. Sesamin significantly attenuated TNF-alpha-induced ICAM-1 expression and cell adhesion by downregulation of extracellular signal-regulated kinase 1/2 and p38. Furthermore, in vivo, sesamin attenuated intimal thickening and ICAM-1 expression seen in aortas of apolipoprotein-E-deficient mice. Taken together, these data suggest that sesamin inhibits TNF-alpha-induced extracellular signal-regulated kinase/p38 phosphorylation, nuclear translocation of NF-kappaB p65, cytoplasmic translocalization of HuR and thereby suppresses ICAM-1 expression, resulting in reduced adhesion of leukocytes. These results also suggest that sesamin may prevent the development of atherosclerosis and inflammatory responses. PMID:20306475

Wu, Wen-Huey; Wang, Shu-Huei; Kuan, I-I; Kao, Ya-Shi; Wu, Pei-Jhen; Liang, Chan-Jung; Chien, Hsiung-Fei; Kao, Chiu-Hua; Huang, Ching-Jang; Chen, Yuh-Lien

2010-09-01

87

Interferon gamma regulates platelet endothelial cell adhesion molecule 1 expression and neutrophil infiltration into herpes simplex virus- infected mouse corneas  

PubMed Central

In a mouse model of herpes simplex virus (HSV) 1 corneal infection, tissue destruction results from a CD4+ T cell-mediated chronic inflammation, in which interleukin 2 and interferon (IFN) gamma are requisite inflammatory mediators and polymorphonuclear leukocytes (PMN) are the predominant infiltrating cells. In vivo neutralization of IFN- gamma relieved inflammation at least in part through a specific block of PMN extravasation into HSV-1-infected corneas. Intercellular adhesion molecule (ICAM) 1 and platelet endothelial cell adhesion molecule (PECAM) 1 were upregulated on the vascular endothelium of inflamed corneas. Reduced PMN extravasation in anti-IFN-gamma-treated mice was associated with a dramatic reduction of PECAM-1 but not ICAM-1 expression on vascular endothelium. PMN accumulated in the lumen of corneal vessels after in vivo IFN-gamma neutralization. PECAM-1 was readily detectable on PMN inside the vessels but was not detectable on PMN that extravasated into the infected cornea. Moreover, flow cytometric analysis revealed reduced PECAM-1 expression but elevated major histocompatibility complex class I expression on PMN that recently extravasated into the peritoneal cavity when compared with PMN in the peripheral blood. We conclude that IFN-gamma contributes to HSV- 1-induced corneal inflammation by facilitating PMN infiltration; this appears to be accomplished through upregulation of PECAM-1 expression on the vascular endothelium; and PMN downregulate PECAM-1 expression during the process of extravasation. PMID:8879215

1996-01-01

88

Transcriptional activation of the vascular cell adhesion molecule-1 gene in T lymphocytes expressing human T-cell leukemia virus type 1 Tax protein.  

PubMed Central

Recruitment and extravasation of T cells through the blood-brain barrier are favored by adhesion molecule-mediated interactions of circulating T cells with endothelial cells. Since a common pathological finding in human T-cell leukemia virus type 1 (HTLV-1)-associated diseases is the infiltration of HTLV-1-infected T lymphocytes into various organs, we have looked for the profile of adhesion molecules expressed by HTLV-1-transformed T cells. Flow cytometry analysis indicated that these cells were expressing high levels of vascular cell adhesion molecule 1 (VCAM-1 [CD106]), a 110-kDa member of the immunoglobulin gene superfamily, first identified on endothelial cells stimulated with inflammatory cytokines. This adhesion molecule was also expressed by T cells obtained from one patient with HTLV-1-associated myelopathy/tropical spastic paraparesis but not by activated T cells isolated from one normal blood donor. The role of the viral trans-activator Tax protein in the induction of VCAM-1 was first indicated by the detection of this adhesion molecule on Jurkat T-cell clones stably expressing the tax gene. The effect of Tax on VCAM-1 gene transcription was next confirmed in JPX-9 cells, a subclone of Jurkat cells, carrying the tax sequences under the control of an inducible promoter. Furthermore, deletion and mutation analyses of the VCAM-1 promoter performed with chloramphenicol acetyltransferase constructs revealed that Tax was trans activating the VCAM-1 promoter via two NF-kappaB sites present at bp -72 and -57 in the VCAM-1 gene promoter, with both of them being required for the Tax-induced expression of this adhesion molecule. Finally, gel mobility shift assays demonstrated the nuclear translocation of proteins specifically bound to these two NF-kappaB motifs, confirming that VCAM-1 was induced on Tax-expressing cells in a kappaB-dependent manner. Collectively, these results therefore suggest that the exclusive Tax-induced expression of VCAM-1 on T cells may represent a pivotal event in the progression of HTLV-1-associated diseases. PMID:9343210

Valentin, H; Lemasson, I; Hamaia, S; Cassé, H; König, S; Devaux, C; Gazzolo, L

1997-01-01

89

Dileucine and PDZ-binding Motifs Mediate Synaptic Adhesion-like Molecule 1 (SALM1) Trafficking in Hippocampal Neurons*  

PubMed Central

Synaptic adhesion-like molecules (SALMs) are a family of cell adhesion molecules involved in neurite outgrowth and synapse formation. Of the five family members, only SALM1, -2, and -3 contain a cytoplasmic C-terminal PDZ-binding motif. We have found that SALM1 is unique among the SALMs because deletion of its PDZ-binding motif (SALM1?PDZ) blocks its surface expression in heterologous cells. When expressed in hippocampal neurons, SALM1?PDZ had decreased surface expression in dendrites and the cell soma but not in axons, suggesting that the PDZ-binding domain may influence cellular trafficking of SALMs to specific neuronal locations. Endoglycosidase H digestion assays indicated that SALM1?PDZ is retained in the endoplasmic reticulum (ER) in heterologous cells. However, when the entire C-terminal tail of SALM1 was deleted, SALM1 was detected on the cell surface. Using serial deletions, we identified a region of SALM1 that contains a putative dileucine ER retention motif, which is not present in the other SALMs. Mutation of this DXXXLL motif allowed SALM1 to leave the ER and enhanced its surface expression in heterologous cells and neurons. An increase in the number of protrusions at the dendrites and cell body was observed when this SALM1 mutant was expressed in hippocampal neurons. With electron microscopy, these protrusions appeared to be irregular, enlarged spines and filopodia. Thus, enrichment of SALM1 on the cell surface affects dendritic arborization, and intracellular motifs regulate its dendritic versus axonal localization. PMID:22174418

Seabold, Gail K.; Wang, Philip Y.; Petralia, Ronald S.; Chang, Kai; Zhou, Arthur; McDermott, Mark I.; Wang, Ya-Xian; Milgram, Sharon L.; Wenthold, Robert J.

2012-01-01

90

Dileucine and PDZ-binding motifs mediate synaptic adhesion-like molecule 1 (SALM1) trafficking in hippocampal neurons.  

PubMed

Synaptic adhesion-like molecules (SALMs) are a family of cell adhesion molecules involved in neurite outgrowth and synapse formation. Of the five family members, only SALM1, -2, and -3 contain a cytoplasmic C-terminal PDZ-binding motif. We have found that SALM1 is unique among the SALMs because deletion of its PDZ-binding motif (SALM1?PDZ) blocks its surface expression in heterologous cells. When expressed in hippocampal neurons, SALM1?PDZ had decreased surface expression in dendrites and the cell soma but not in axons, suggesting that the PDZ-binding domain may influence cellular trafficking of SALMs to specific neuronal locations. Endoglycosidase H digestion assays indicated that SALM1?PDZ is retained in the endoplasmic reticulum (ER) in heterologous cells. However, when the entire C-terminal tail of SALM1 was deleted, SALM1 was detected on the cell surface. Using serial deletions, we identified a region of SALM1 that contains a putative dileucine ER retention motif, which is not present in the other SALMs. Mutation of this DXXXLL motif allowed SALM1 to leave the ER and enhanced its surface expression in heterologous cells and neurons. An increase in the number of protrusions at the dendrites and cell body was observed when this SALM1 mutant was expressed in hippocampal neurons. With electron microscopy, these protrusions appeared to be irregular, enlarged spines and filopodia. Thus, enrichment of SALM1 on the cell surface affects dendritic arborization, and intracellular motifs regulate its dendritic versus axonal localization. PMID:22174418

Seabold, Gail K; Wang, Philip Y; Petralia, Ronald S; Chang, Kai; Zhou, Arthur; McDermott, Mark I; Wang, Ya-Xian; Milgram, Sharon L; Wenthold, Robert J

2012-02-10

91

Platelet Endothelial Cell Adhesion Molecule-1, a Putative Receptor for the Adhesion of Streptococcus pneumoniae to the Vascular Endothelium of the Blood-Brain Barrier  

PubMed Central

The Gram-positive bacterium Streptococcus pneumoniae is the main causative agent of bacterial meningitis. S. pneumoniae is thought to invade the central nervous system via the bloodstream by crossing the vascular endothelium of the blood-brain barrier. The exact mechanism by which pneumococci cross endothelial cell barriers before meningitis develops is unknown. Here, we investigated the role of PECAM-1/CD31, one of the major endothelial cell adhesion molecules, in S. pneumoniae adhesion to vascular endothelium of the blood-brain barrier. Mice were intravenously infected with pneumococci and sacrificed at various time points to represent stages preceding meningitis. Immunofluorescent analysis of brain tissue of infected mice showed that pneumococci colocalized with PECAM-1. In human brain microvascular endothelial cells (HBMEC) incubated with S. pneumoniae, we observed a clear colocalization between PECAM-1 and pneumococci. Blocking of PECAM-1 reduced the adhesion of S. pneumoniae to endothelial cells in vitro, implying that PECAM-1 is involved in pneumococcal adhesion to the cells. Furthermore, using endothelial cell protein lysates, we demonstrated that S. pneumoniae physically binds to PECAM-1. Moreover, both in vitro and in vivo PECAM-1 colocalizes with the S. pneumoniae adhesion receptor pIgR. Lastly, immunoprecipitation experiments revealed that PECAM-1 can physically interact with pIgR. In summary, we show for the first time that blood-borne S. pneumoniae colocalizes with PECAM-1 expressed by brain microvascular endothelium and that, in addition, they colocalize with pIgR. We hypothesize that this interaction plays a role in pneumococcal binding to the blood-brain barrier vasculature prior to invasion into the brain. PMID:24914219

Iovino, Federico; Molema, Grietje

2014-01-01

92

Normal and shear stresses influence the spatial distribution of intracellular adhesion molecule-1 expression in human umbilical vein endothelial cells exposed to sudden expansion flow.  

PubMed

Patterns in cell adhesion molecule expression by endothelial cells may play a role in atherogenesis. Previous studies have shown dependence of intracellular adhesion molecule-1 (ICAM-1) expression in human umbilical vein endothelial cells (HUVEC) on shear stress and have indirectly linked ICAM-1 expression to spatial gradients in shear stress. The spatial distribution of ICAM-1 in HUVEC pre-exposed to flow for 8h was determined using fluorescence microscopy and a sudden expansion flow chamber with a 2.66 expansion ratio to simulate gradients in wall shear stress found near arterial branches in vivo. When ICAM-1 expression in the disturbed flow region was compared to theoretical stress distributions obtained from a computational model of sudden expansion flow, a modest trend (R2 = 0.327, p < 0.01)was observed between ICAM-1 and shear stress but the correlation between ICAM-1 and shear stress gradient was insignificant. In contrast, a moderately strong trend (R2 = 0.873, p < 0.01) was evident between ICAM-1 expression and the component of normal stress induced by the expansion. Thus, in this in vitro model, normal stress arising from sudden expansion flow modulates the effect of shear stress on ICAM-1 expression. PMID:16488220

McKinney, V Z; Rinker, K D; Truskey, G A

2006-01-01

93

Calcium mobilization and Rac1 activation are required for VCAM-1 (vascular cell adhesion molecule-1) stimulation of NADPH oxidase activity.  

PubMed Central

VCAM-1 (vascular cell adhesion molecule-1) plays an important role in the regulation of inflammation in atherosclerosis, asthma, inflammatory bowel disease and transplantation. VCAM-1 activates endothelial cell NADPH oxidase, and this oxidase activity is required for VCAM-1-dependent lymphocyte migration. We reported previously that a mouse microvascular endothelial cell line promotes lymphocyte migration that is dependent on VCAM-1, but not on other known adhesion molecules. Here we have investigated the signalling mechanisms underlying VCAM-1 function. Lymphocyte binding to VCAM-1 on the endothelial cell surface activated an endothelial cell calcium flux that could be inhibited with anti-alpha4-integrin and mimicked by anti-VCAM-1-coated beads. VCAM-1 stimulation of calcium responses could be blocked by an inhibitor of intracellular calcium mobilization, a calcium channel inhibitor or a calcium chelator, resulting in the inhibition of NADPH oxidase activity. Addition of ionomycin overcame the calcium channel blocker suppression of VCAM-1-stimulated NADPH oxidase activity, but could not reverse the inhibitory effect imposed by intracellular calcium blockage, indicating that both intracellular and extracellular calcium mobilization are required for VCAM-1-mediated activation of NADPH oxidase. Furthermore, VCAM-1 specifically activated the Rho-family GTPase Rac1, and VCAM-1 activation of NADPH oxidase was blocked by a dominant negative Rac1. Thus VCAM-1 stimulates the mobilization of intracellular and extracellular calcium and Rac1 activity that are required for the activation of NADPH oxidase. PMID:14594451

Cook-Mills, Joan M; Johnson, Jacob D; Deem, Tracy L; Ochi, Atsuo; Wang, Lei; Zheng, Yi

2004-01-01

94

Artemether combined with shRNA interference of vascular cell adhesion molecule-1 significantly inhibited the malignant biological behavior of human glioma cells.  

PubMed

Artemether is the derivative extracted from Chinese traditional herb and originally used for malaria. Artemether also has potential therapeutic effects against tumors. Vascular cell adhesion molecule-1 (VCAM-1) is an important cell surface adhesion molecule associated with malignancy of gliomas. In this work, we investigated the role and mechanism of artemether combined with shRNA interference of VCAM-1 (shRNA-VCAM-1) on the migration, invasion and apoptosis of glioma cells. U87 human glioma cells were treated with artemether at various concentrations and shRNA interfering technology was employed to silence the expression of VCAM-1. Cell viability, migration, invasiveness and apoptosis were assessed with MTT, wound healing, Transwell and Annexin V-FITC/PI staining. The expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and phosphorylated Akt (p-Akt) was checked by Western blot assay. Results showed that artemether and shRNA-VCAM-1 not only significantly inhibited the migration, invasiveness and expression of MMP-2/9 and p-Akt, but also promoted the apoptosis of U87 cells. Combined treatment of both displayed the maximum inhibitory effects on the malignant biological behavior of glioma cells. Our work revealed the potential therapeutic effects of artemether and antiVCAM-1 in the treatments of gliomas. PMID:23593320

Wang, Ying-Bin; Hu, Yi; Li, Zhen; Wang, Ping; Xue, Yi-Xue; Yao, Yi-Long; Yu, Bo; Liu, Yun-Hui

2013-01-01

95

FRET Based Quantification and Screening Technology Platform for the Interactions of Leukocyte Function-Associated Antigen-1 (LFA-1) with InterCellular Adhesion Molecule-1 (ICAM-1)  

PubMed Central

The interaction between leukocyte function-associated antigen-1(LFA-1) and intercellular adhesion molecule-1 (ICAM-1) plays a pivotal role in cellular adhesion including the extravasation and inflammatory response of leukocytes, and also in the formation of immunological synapse. However, irregular expressions of LFA-1 or ICAM-1 or both may lead to autoimmune diseases, metastasis cancer, etc. Thus, the LFA-1/ICAM-1 interaction may serve as a potential therapeutic target for the treatment of these diseases. Here, we developed one simple ‘in solution’ steady state fluorescence resonance energy transfer (FRET) technique to obtain the dissociation constant (Kd) of the interaction between LFA-1 and ICAM-1. Moreover, we developed the assay into a screening platform to identify peptides and small molecules that inhibit the LFA-1/ICAM-1 interaction. For the FRET pair, we used Alexa Fluor 488-LFA-1 conjugate as donor and Alexa Fluor 555-human recombinant ICAM-1 (D1-D2-Fc) as acceptor. From our quantitative FRET analysis, the Kd between LFA-1 and D1-D2-Fc was determined to be 17.93±1.34 nM. Both the Kd determination and screening assay were performed in a 96-well plate platform, providing the opportunity to develop it into a high-throughput assay. This is the first reported work which applies FRET based technique to determine Kd as well as classifying inhibitors of the LFA-1/ICAM-1 interaction. PMID:25032811

Chakraborty, Sandeep; Núñez, David; Hu, Shih-Yang; Domingo, María Pilar; Pardo, Julian; Karmenyan, Artashes; Chiou, Arthur

2014-01-01

96

Monocyte Trafficking to Hepatic Sites of Bacterial Infection Is Chemokine Independent and Directed by Focal Intercellular Adhesion Molecule-1 Expression  

PubMed Central

Recruitment of CCR2+Ly6Chigh monocytes to sites of infection is essential for efficient clearance of microbial pathogens. Although CCR2-mediated signals promote monocyte emigration from bone marrow, the contribution of CCR2 to later stages of monocyte recruitment remains unresolved. In this article, we show that CCR2 deficiency markedly worsens hepatic Listeria monocytogenes infection because Ly6Chigh monocytes are retained in the bone marrow. Intravenously transferred, CCR2-deficient Ly6Chigh monocytes traffic normally to hepatic foci of infection and contribute to bacterial clearance. Pertussis toxin treatment of adoptively transferred monocytes does not impair their intrahepatic trafficking, suggesting that chemokine signaling, once CCR2+ Ly6Chigh monocytes emigrate from the bone marrow, is not required for monocyte localization to sites of bacterial infection in the liver. Expression of ICAM-1 is induced in close proximity to foci of bacterial infection in the liver, including on CD31+ endothelial cells, and blockade of CD11b and CD44 diminishes monocyte localization to these hepatic foci. Our studies demonstrated that Ly6Chigh monocyte recruitment from the bloodstream to the L. monocytogenes-infected liver does not require chemokine receptor-mediated signals but instead is principally dependent on integrin- and extracellular matrix-mediated monocyte adhesion. PMID:20435926

Shi, Chao; Velázquez, Peter; Hohl, Tobias M.; Leiner, Ingrid; Dustin, Michael L.; Pamer, Eric G.

2010-01-01

97

GroEL1, from Chlamydia pneumoniae, Induces Vascular Adhesion Molecule 1 Expression by p37AUF1 in Endothelial Cells and Hypercholesterolemic Rabbit  

PubMed Central

The expression of vascular adhesion molecule-1 (VCAM-1) by endothelial cells may play a major role in atherogenesis. The actual mechanisms of chlamydia pneumoniae (C. pneumoniae) relate to atherogenesis are unclear. We investigate the influence of VCAM-1 expression in the GroEL1 from C. pneumoniae-administered human coronary artery endothelial cells (HCAECs) and hypercholesterolemic rabbits. In this study, we constructed the recombinant GroEL1 from C. pneumoniae. The HCAECs/THP-1 adhesion assay, tube formation assay, western blotting, enzyme-linked immunosorbent assay, actinomycin D chase experiment, luciferase reporter assay, and immunohistochemical stainings were performed. The results show that GroEL1 increased both VCAM-1expression and THP-1 cell adhesives, and impaired tube-formation capacity in the HCAECs. GroEL1 significantly increased the VCAM-1 mRNA stability and cytosolic AU-binding factor 1 (AUF1) level. Overexpression of the p37AUF1 significantly increased VCAM-1 gene expression in GroEL1-induced bovine aortic endothelial cells (BAECs). GroEL1 prolonged the stability of VCAM-1 mRNA by increasing both p37AUF1 and the regulation of the 5? untranslated region (UTR) of the VCAM-1 mRNA in BAECs. In hypercholesterolemic rabbits, GroEL1 administration enhanced fatty-streak and macrophage infiltration in atherosclerotic lesions, which may be mediated by elevated VCAM-1 expression. In conclusion, GroEL1 induces VCAM-1 expression by p37AUF1 in endothelial cells and enhances atherogenesis in hypercholesterolemic rabbits. PMID:22900050

Shih, Chun-Ming; Tsao, Nai-Wen; Chen, Yung-Hsiang; Li, Chi-Yuan; Chang, Yu-Jia; Chang, Nen-Chung; Ou, Keng-Liang; Lin, Cheng-Yen; Nien, Chih-Hao; Lin, Feng-Yen

2012-01-01

98

Simple modifications to methimazole that enhance its inhibitory effect on tumor necrosis factor-?-induced vascular cell adhesion molecule-1 expression by human endothelial cells.  

PubMed

The expression of vascular cell adhesion molecule-1 (VCAM-1) on the vascular endothelium can be increased by pro-inflammatory cytokines [e.g. tumor necrosis factor-? (TNF-?)]. VCAM-1 contributes to leukocyte adhesion to, and emigration from, the vasculature which is a key aspect of pathological inflammation. As such, a promising therapeutic approach for pathological inflammation is to inhibit the expression of VCAM-1. Methimazole [3-methyl-1, 3 imidazole-2 thione (MMI)] is routinely used for the treatment of Graves? disease and patients treated with MMI have decreased levels of circulating VCAM-1. In this study we used cultured human umbilical vein endothelial cells (HUVEC) to investigate the effect of MMI structural modifications on TNF-? induced VCAM-1 expression. We found that addition of a phenyl ring at the 4-nitrogen of MMI yields a compound that is significantly more potent than MMI at inhibiting 24h TNF-?-induced VCAM-1 protein expression. Addition of a para methoxy to the appended phenyl group increases the inhibition while substitution of a thiazole ring for an imidazole ring in the phenyl derivatives yields no clear difference in inhibition. Addition of the phenyl ring to MMI appears to increase toxicity as does substitution of a thiazole ring for an imidazole ring in the phenyl MMI derivatives. Each of the compounds reduced TNF-?-induced VCAM-1 mRNA expression and had a functional inhibitory effect, i.e. each inhibited monocytic cell adhesion to 24h TNF-?-activated HUVEC under fluid flow conditions. Combined, these studies provide important insights into the design of MMI-related anti-inflammatory compounds. PMID:25641748

Alapati, Anuja; Deosarkar, Sudhir P; Lanier, Olivia L; Qi, Chunyan; Carlson, Grady E; Burdick, Monica M; Schwartz, Frank L; McCall, Kelly D; Bergmeier, Stephen C; Goetz, Douglas J

2015-03-15

99

Nectin-like molecule 1 is a glycoprotein with a single N-glycosylation site at N290KS which influences its adhesion activity.  

PubMed

Nectin-like molecule 1 (NECL1)/CADM3/IGSF4B/TSLL1/SynCAM3, from now on referred to as NECL1, is a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule which has Ca(2+)-independent homo- or heterophilic cell-cell adhesion activity and plays an important role in the formation of synapses, axon bundles and myelinated axons. Here we first detected the expression of NECL1 in human fetal and adult brains, and mouse brains at different developmental stages. The results indicated that two bands with molecular weights of about 62 kDa and 48 kDa were found in human fetal brain, while only one band with a molecular weight of about 48 kDa was found in human adult brain; two bands with molecular weights of about 62 kDa and 48 kDa whose expression level gradually increased were also found from mouse E16 to P14, while only one band with a molecular weight of about 48 kDa was found from P14. Bioinformatics analysis showed there were two putative N-glycosylation sites within human NECL1 at positions N25LS and N290KS and within mouse Necl1 at positions N23LS and N288KS, respectively. There was no O-glycosylation site in either human NECL1 or mouse Necl1. Based on the results of N-Glycosidase F treatment with human fetal brain tissue and lysates from transient transfection with human wild-type or glycosylation site mutant NECL1 in 293ET cells, we demonstrated that human NECL1 is an N-linked glycoprotein with a single glycosylation site at position N290KS. Cell aggregation assay further showed there was an increased adhesion activity after the glycosylation site mutation of NECL1 molecule. PMID:18420026

Gao, Jing; Chen, Tao; Hu, Guangyu; Gong, Yanhua; Qiang, Boqin; Yuan, Jiangang; Peng, Xiaozhong

2008-06-01

100

The Interaction Affinity between Vascular Cell Adhesion Molecule-1 (VCAM-1) and Very Late Antigen-4 (VLA-4) Analyzed by Quantitative FRET  

PubMed Central

Very late antigen-4 (VLA-4), a member of integrin superfamily, interacts with its major counter ligand vascular cell adhesion molecule-1 (VCAM-1) and plays an important role in leukocyte adhesion to vascular endothelium and immunological synapse formation. However, irregular expressions of these proteins may also lead to several autoimmune diseases and metastasis cancer. Thus, quantifying the interaction affinity of the VCAM-1/VLA-4 interaction is of fundamental importance in further understanding the nature of this interaction and drug discovery. In this study, we report an ‘in solution’ steady state organic fluorophore based quantitative fluorescence resonance energy transfer (FRET) assay to quantify this interaction in terms of the dissociation constant (Kd). We have used, in our FRET assay, the Alexa Fluor 488-VLA-4 conjugate as the donor, and Alexa Fluor 546-VCAM-1 as the acceptor. From the FRET signal analysis, Kd of this interaction was determined to be 41.82 ± 2.36 nM. To further confirm our estimation, we have employed surface plasmon resonance (SPR) technique to obtain Kd = 39.60 ± 1.78 nM, which is in good agreement with the result obtained by FRET. This is the first reported work which applies organic fluorophore based ‘in solution’ simple quantitative FRET assay to obtain the dissociation constant of the VCAM-1/VLA-4 interaction, and is also the first quantification of this interaction. Moreover, the value of Kd can serve as an indicator of abnormal protein-protein interactions; hence, this assay can potentially be further developed into a drug screening platform of VLA-4/VCAM-1 as well as other protein-ligand interactions. PMID:25793408

Wu, Shu-Han; Karmenyan, Artashes; Chiou, Arthur

2015-01-01

101

Epidermal Growth Factor (EGF)-enhanced Vascular Cell Adhesion Molecule-1 (VCAM-1) Expression Promotes Macrophage and Glioblastoma Cell Interaction and Tumor Cell Invasion*  

PubMed Central

Activated EGF receptor (EGFR) signaling plays an instrumental role in glioblastoma (GBM) progression. However, how EGFR activation regulates the tumor microenvironment to promote GBM cell invasion remains to be clarified. Here, we demonstrate that the levels of EGFR activation in tumor cells correlated with the levels of macrophage infiltration in human GBM specimens. This was supported by our observation that EGFR activation enhanced the interaction between macrophages and GBM cells. In addition, EGF treatment induced up-regulation of vascular cell adhesion molecule-1 (VCAM-1) expression in a PKC?- and NF-?B-dependent manner. Depletion of VCAM-1 interrupted the binding of macrophages to GBM cells and inhibited EGF-induced and macrophage-promoted GBM cell invasion. These results demonstrate an instrumental role for EGF-induced up-regulation of VCAM-1 expression in EGFR activation-promoted macrophage-tumor cell interaction and tumor cell invasion and indicate that VCAM-1 is a potential molecular target for improving cancer therapy. PMID:24045955

Zheng, Yanhua; Yang, Weiwei; Aldape, Kenneth; He, Jie; Lu, Zhimin

2013-01-01

102

Soluble Intercellular Adhesion Molecules, Soluble Vascular Cell Adhesion Molecules, and Risk of Coronary Heart Disease  

Microsoft Academic Search

Objective: We examined the association of circulating levels of soluble intercellular adhesion molecules (sICAM-1) and soluble vascular cell adhesion molecules (sVCAM-1) with coronary heart disease (CHD) risk factors and whether the adhesion molecules alone, and in combination, can serve as predictors of coronary CHD.Research Methods and Procedures: Among 18,225 men from the Health Professional Follow-up Study who provided blood in

Iris Shai; Tobias Pischon; Frank B. Hu; Alberto Ascherio; Nader Rifai; Eric B. Rimm

2006-01-01

103

Heparan Sulfates Mediate the Interaction between Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) and the G?q/11 Subunits of Heterotrimeric G Proteins*  

PubMed Central

The endothelial cell-cell junction has emerged as a major cell signaling structure that responds to shear stress by eliciting the activation of signaling pathways. Platelet endothelial cell adhesion molecule-1 (PECAM-1) and heterotrimeric G protein subunits G?q and 11 (G?q/11) are junctional proteins that have been independently proposed as mechanosensors. Our previous findings suggest that they form a mechanosensitive junctional complex that discriminates between different flow profiles. The nature of the PECAM-1·G?q/11 interaction is still unclear although it is likely an indirect association. Here, we investigated the role of heparan sulfates (HS) in mediating this interaction and in regulating downstream signaling in response to flow. Co-immunoprecipitation studies show that PECAM-1·G?q/11 binding is dramatically decreased by competitive inhibition with heparin, pharmacological inhibition with the HS antagonist surfen, and enzymatic removal of HS chains with heparinase III treatment as well as by site-directed mutagenesis of basic residues within the extracellular domain of PECAM-1. Using an in situ proximity ligation assay, we show that endogenous PECAM-1·G?q/11 interactions in endothelial cells are disrupted by both competitive inhibition and HS degradation. Furthermore, we identified the heparan sulfate proteoglycan syndecan-1 in complexes with PECAM-1 that are rapidly decreased in response to flow. Finally, we demonstrate that flow-induced Akt activation is attenuated in endothelial cells in which PECAM-1 was knocked down and reconstituted with a binding mutant. Taken together, our results indicate that the PECAM-1·G?q/11 mechanosensitive complex contains an endogenous heparan sulfate proteoglycan with HS chains that is critical for junctional complex assembly and regulating the flow response. PMID:24497640

dela Paz, Nathaniel G.; Melchior, Benoît; Shayo, Francisca Y.; Frangos, John A.

2014-01-01

104

Vascular and extravascular immunoreactivity for Intercellular Adhesion Molecule 1 in the orbitofrontal cortex of subjects with major depression: age-dependent changes  

PubMed Central

Background Vascular and immune alterations in the prefrontal cortex may contribute to major depression in elderly subjects. Intercellular adhesion molecule-1 (ICAM-1), major inflammatory mediator in vessels and astrocytes, could be altered in geriatric depression, but little is known about its age-dependent expression in subjects with depression and its relationship to astrocytes identified by the marker glial fibrillary acidic protein (GFAP), found to be reduced in depression. Methods We measured the percentage of gray matter area fraction covered by ICAM-1 immunoreactivity in blood vessels and in extravascular accumulations of ICAM-1 immunoreactivity in 19 non-psychiatric comparison subjects and 18 subjects with major depression, all characterized by postmortem psychological diagnosis. Association of extravascular ICAM-1 to GFAP-positive astrocytes was investigated by double-labeling immunofluorescence. Results Vascular and extravascular fractions of ICAM-1 immunoreactivity were lower in subjects with MDD than in non-psychiatric comparison subjects. Non-psychiatric comparison subjects older than 60 experienced dramatic increase in extravascular ICAM-1 immunoreactivity, but this increase was attenuated in elderly subjects with MDD, particularly in those dying by suicide. Most extracellular ICAM-1 immunoreactivity was coextensive with GFAP-immunoreactive astrocytes in both groups. Limitations Heterogeneity in type and dosage of antidepressant medication. Difficulty in determining the exact onset of depression in subjects older than 60 at the time of death. Routine cerebrovascular pathological screening may miss subtle subcellular and molecular changes. Conclusions There is significant attenuation of extravascular and vascular ICAM-1 immunoreactivity in elderly subjects with major depression suggesting an astrocyte-associated alteration in immune function in the aging orbitofrontal cortex of subjects with MDD. PMID:21536333

Miguel-Hidalgo, Jose Javier; Overholser, James C.; Jurjus, George J.; Meltzer, Herbert Y.; Dieter, Lesa; Konick, Lisa; Stockmeier, Craig A.; Rajkowska, Grazyna

2011-01-01

105

Heparan sulfates mediate the interaction between platelet endothelial cell adhesion molecule-1 (PECAM-1) and the G?q/11 subunits of heterotrimeric G proteins.  

PubMed

The endothelial cell-cell junction has emerged as a major cell signaling structure that responds to shear stress by eliciting the activation of signaling pathways. Platelet endothelial cell adhesion molecule-1 (PECAM-1) and heterotrimeric G protein subunits G?q and 11 (G?q/11) are junctional proteins that have been independently proposed as mechanosensors. Our previous findings suggest that they form a mechanosensitive junctional complex that discriminates between different flow profiles. The nature of the PECAM-1·G?q/11 interaction is still unclear although it is likely an indirect association. Here, we investigated the role of heparan sulfates (HS) in mediating this interaction and in regulating downstream signaling in response to flow. Co-immunoprecipitation studies show that PECAM-1·G?q/11 binding is dramatically decreased by competitive inhibition with heparin, pharmacological inhibition with the HS antagonist surfen, and enzymatic removal of HS chains with heparinase III treatment as well as by site-directed mutagenesis of basic residues within the extracellular domain of PECAM-1. Using an in situ proximity ligation assay, we show that endogenous PECAM-1·G?q/11 interactions in endothelial cells are disrupted by both competitive inhibition and HS degradation. Furthermore, we identified the heparan sulfate proteoglycan syndecan-1 in complexes with PECAM-1 that are rapidly decreased in response to flow. Finally, we demonstrate that flow-induced Akt activation is attenuated in endothelial cells in which PECAM-1 was knocked down and reconstituted with a binding mutant. Taken together, our results indicate that the PECAM-1·G?q/11 mechanosensitive complex contains an endogenous heparan sulfate proteoglycan with HS chains that is critical for junctional complex assembly and regulating the flow response. PMID:24497640

dela Paz, Nathaniel G; Melchior, Benot; Shayo, Francisca Y; Frangos, John A

2014-03-14

106

Evidence that DNA Damage Is a Mediate in Ultraviolet B Radiation-Induced Inhibition of Human Gene Expression: Ultraviolet B Radiation Effects on Intercellular Adhesion Molecule1 (ICAM-1) Expression  

Microsoft Academic Search

Expression of intercellular adhesion molecule-1 (ICAM-1) is a prerequisite for the capacity of cells to physically interact with leukocytes. Ultraviolet B radiation previously was found to inhibit interferon ?-induced ICAM-1 expression in human keratinocytes by suppressing interferon ?-mediated upregulation of ICAM-1 mRNA levels. Because ultraviolet B radiation induces photoproducts in cellular DNA, the potential role of ultraviolet B radiation-induced DNA

Jean Krutmann; Elisabeth Bohnert; Ernst G. Jung

1994-01-01

107

Plasma asymmetric dimethylarginine (ADMA) concentration is independently associated with carotid intima-media thickness and plasma soluble vascular cell adhesion molecule-1 (sVCAM-1) concentration in patients with mild-to-moderate renal failure  

Microsoft Academic Search

Plasma asymmetric dimethylarginine (ADMA) concentration is independently associated with carotid intima-media thickness and plasma soluble vascular cell adhesion molecule-1 (sVCAM-1) concentration in patients with mild-to-moderate renal failure.BackgroundPatients with renal insufficiency have an increased risk of cardiovascular disease that is not fully explained by the presence of known cardiovascular risk factors. In patients with end-stage renal disease, increased serum concentration of

PRABATH W B NANAYAKKARA; TOM TEERLINK; COEN D A STEHOUWER; DAUD ALLAJAR; ANNEMIEKE SPIJKERMAN; CASPER SCHALKWIJK; PIET M TER WEE; COEN VAN GULDENER

2005-01-01

108

Adenosine A 2A analogue ATL-146e reduces systemic tumor necrosing factor-? and spinal cord capillary platelet-endothelial cell adhesion molecule-1 expression after spinal cord ischemia  

Microsoft Academic Search

Objective: Inflammation is likely a major contributor to spinal cord reperfusion injury after aortic reconstruction. Systemic 4-{3-[6-amino-9-(5-ethylcarbamoyl-3,4-dihydroxy-tetrahydro-furan-2-yl)-9H-purin-2-yl]-prop-2-ynyl}-cyclohexanecarboxylic acid methyl ester (ATL-146e), a selective adenosine A2A agonist, has been shown to reduce paralysis after spinal cord ischemia. We hypothesized that ATL-146e reduces cytokine production during spinal cord reperfusion, curtailing inflammation and decreasing spinal cord capillary platelet-endothelial cell adhesion molecule-1 (PECAM-1) expression.

David C. Cassada; Curtis G. Tribble; Stewart M. Long; Victor E. Laubach; Aditya K. Kaza; Joel Linden; Bao-Ngoc Nguyen; Jayson M. Rieger; Steven M. Fiser; Irving L. Kron; John A. Kern

2002-01-01

109

C-Reactive Protein and Soluble Vascular Cell Adhesion Molecule1 Are Associated With Elevated Urinary Albumin Excretion but Do Not Explain Its Link With Cardiovascular Risk  

Microsoft Academic Search

An elevated urinary albumin excretion rate (UAER) is associated with an increased risk of cardiovascular mortality, but the pathophysiological mechanism underlying this association is poorly understood. To investigate the role of endothelial dysfunction, leukocyte adhesion, and low-grade inflammation (1) in the development of elevated UAER (study I) and (2) in linking elevated UAER with risk of cardiovascular mortality (study II),

Agnes Jager; Victor W. M. van Hinsbergh; Piet J. Kostense; Jef J. Emeis; Giel Nijpels; Jacqueline M. Dekker; Robert J. Heine; Lex M. Bouter; Coen D. A. Stehouwer

110

Arrangement of Domains, and Amino Acid Residues Required for Binding of Vascular Cell Adhesion Molecule1 to Its Counter-Receptor VLA4 (a4fll)  

Microsoft Academic Search

Interaction of the vascular cell adhesion molecule (VCAM-1) with its counter-receptor very late antigen-4 (VLA-4) (integrin ot4fl0 is important for a number of developmental pathways and inflammatory functions. We are investigating the molecular mecha- nism of this binding, in the interest of developing new anti-inflammatory drugs that block it. In a previous report, we showed that the predominant form of

Laurelee Osborn; Cornelia Vassallo; Beth Grifliths Browning; Richard Tizard; Dorian O. Haskard; Christopher D. Benjamin; Irene Dougas; Tomas Kirchhausen

1994-01-01

111

N-acetyl-D-galactosamine specific lectin of Eikenella corrodens induces intercellular adhesion molecule-1 (ICAM-1) production by human oral epithelial cells.  

PubMed

During the acute inflammatory response in periodontitis, gingival epithelial cells are considered to play important roles in the recruitment of inflammatory cells to the site of infection through the secretion of chemokines. However, little is known about the expression of molecules that are involved in the interaction between the epithelium and neutrophils following bacterial attachment. Earlier work reported that periodontopathogenic Eikenella corrodens strain 1,073 up-regulated the expression and secretion of chemokines such as interleukin-8 (IL-8) from KB cells (a human oral epithelial cell line derived from a human oral epidermoid carcinoma). To elucidate the mechanism of the transmigration of neutrophils through the epithelium, the present study investigated the expression of adhesion molecules on KB cells in response to E. corrodens attachment. Adhesion molecule gene expression was assessed by RT-PCR and adhesion proteins expressed on KB cell surfaces were determined by cell-based ELISA and FACS. In RT-PCR, ICAM-1 mRNA levels were significantly increased within 1 h in response to exposure to E. corrodens and continued to increase over the 12-h period of study. In ELISA, increased surface ICAM-1 expression was paralleled by increased ICAM-1 mRNA levels. Furthermore, the increases in ICAM-1 expression on epithelial cells infected with E. corrodens were observed to be due to the N-acetyl-D-galactosamine (GalNAc) specific bacterial lectin-like substance of E. corrodens (EcLS), which was one of the adhesins of E. corrodens. This is the first study to report that a bacterial lectin-like substance increased the expression of ICAM-1 on gingival epithelial cells. PMID:12466406

Yamada, Masayoshi; Nakae, Hideaki; Yumoto, Hiromichi; Shinohara, Chihiro; Ebisu, Shigeyuki; Matsuo, Takashi

2002-12-01

112

Pentosan polysulfate treatment ameliorates motor function with increased serum soluble vascular cell adhesion molecule-1 in HTLV-1-associated neurologic disease.  

PubMed

The main therapeutic strategy against human T lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) characterized by lower extremity motor dysfunction is immunomodulatory treatment, with drugs such as corticosteroid hormone and interferon-?, at present. However, there are many issues in long-term treatment with these drugs, such as insufficient effects and various side effects. We now urgently need to develop other therapeutic strategies. The heparinoid, pentosan polysulfate sodium (PPS), has been safely used in Europe for the past 50 years as a thrombosis prophylaxis and for the treatment of phlebitis. We conducted a clinical trial to test the effect of subcutaneous administration of PPS in 12 patients with HAM/TSP in an open-labeled design. There was a marked improvement in lower extremity motor function, based on reduced spasticity, such as a reduced time required for walking 10 m and descending a flight of stairs. There were no significant changes in HTLV-I proviral copy numbers in peripheral blood contrary to the inhibitory effect of PPS in vitro for intercellular spread of HTLV-I. However, serum soluble vascular cell adhesion molecule (sVCAM)-1 was significantly increased without significant changes of serum level of chemokines (CXCL10 and CCL2). There was a positive correlation between increased sVCAM-1and reduced time required for walking 10 m. PPS might induce neurological improvement by inhibition of chronic inflammation in the spinal cord, through blocking the adhesion cascade by increasing serum sVCAM-1, in addition to rheological improvement of the microcirculation. PPS has the potential to be a new therapeutic tool for HAM/TSP. PMID:24671717

Nakamura, Tatsufumi; Satoh, Katsuya; Fukuda, Taku; Kinoshita, Ikuo; Nishiura, Yoshihiro; Nagasato, Kunihiko; Yamauchi, Atsushi; Kataoka, Yasufumi; Nakamura, Tadahiro; Sasaki, Hitoshi; Kumagai, Kenji; Niwa, Masami; Noguchi, Mitsuru; Nakamura, Hideki; Nishida, Noriyuki; Kawakami, Atsushi

2014-06-01

113

Reduced Expression of Adipose Triglyceride Lipase Enhances Tumor Necrosis Factor ?-induced Intercellular Adhesion Molecule-1 Expression in Human Aortic Endothelial Cells via Protein Kinase C-dependent Activation of Nuclear Factor-?B*  

PubMed Central

We examined the effects of adipose triglyceride lipase (ATGL) on the initiation of atherosclerosis. ATGL was recently identified as a rate-limiting triglyceride (TG) lipase. Mutations in the human ATGL gene are associated with neutral lipid storage disease with myopathy, a rare genetic disease characterized by excessive accumulation of TG in multiple tissues. The cardiac phenotype, known as triglyceride deposit cardiomyovasculopathy, shows massive TG accumulation in both coronary atherosclerotic lesions and the myocardium. Recent reports show that myocardial triglyceride content is significantly higher in patients with prediabetes or diabetes and that ATGL expression is decreased in the obese insulin-resistant state. Therefore, we investigated the effect of decreased ATGL activity on the development of atherosclerosis using human aortic endothelial cells. We found that ATGL knockdown enhanced monocyte adhesion via increased expression of TNF?-induced intercellular adhesion molecule-1 (ICAM-1). Next, we determined the pathways (MAPK, PKC, or NF?B) involved in ICAM-1 up-regulation induced by ATGL knockdown. Both phosphorylation of PKC and degradation of I?B? were increased in ATGL knockdown human aortic endothelial cells. In addition, intracellular diacylglycerol levels and free fatty acid uptake via CD36 were significantly increased in these cells. Inhibition of the PKC pathway using calphostin C and GF109203X suppressed TNF?-induced ICAM-1 expression. In conclusion, we showed that ATGL knockdown increased monocyte adhesion to the endothelium through enhanced TNF?-induced ICAM-1 expression via activation of NF?B and PKC. These results suggest that reduced ATGL expression may influence the atherogenic process in neutral lipid storage diseases and in the insulin-resistant state. PMID:21828047

Inoue, Tomoaki; Kobayashi, Kunihisa; Inoguchi, Toyoshi; Sonoda, Noriyuki; Fujii, Masakazu; Maeda, Yasutaka; Fujimura, Yoshinori; Miura, Daisuke; Hirano, Ken-ichi; Takayanagi, Ryoichi

2011-01-01

114

Aryl hydrocarbon receptor (AhR) attenuation of subchronic cigarette smoke-induced pulmonary neutrophilia is associated with retention of nuclear RelB and suppression of intercellular adhesion molecule-1 (ICAM-1).  

PubMed

Cigarette smoke is associated with chronic and enhanced pulmonary inflammation characterized by increased cytokine production and leukocyte recruitment to the lung. Although the aryl hydrocarbon receptor (AhR) is well-known to mediate toxic effects of manmade environmental contaminants, the AhR has emerged as a suppressor of acute cigarette smoke-induced neutrophilia by a mechanism involving the NF-?B protein RelB. Yet individuals who smoke often smoke for many years and vary in their cigarette consumption. As there is currently no information on the AhR prevention of lung inflammation, including neutrophilia, due to varied and prolonged exposure regimes, we exposed control and AhR(-/-) mice to cigarette smoke for 2 weeks (subchronic exposure) utilizing low and high exposure protocols and evaluated pulmonary inflammation. Subchronic cigarette smoke exposure significantly increased pulmonary neutrophilia dose-dependently in AhR(-/-) mice. Surprisingly, there was no difference between smoke-exposed AhR(+/-) and AhR(-/-) mice in the expression of cytokines associated with neutrophil recruitment. Expression of pulmonary intercellular adhesion molecule-1 (ICAM-1), an adhesion molecule involved in neutrophil migration and retention, was higher in pulmonary endothelial cells from AhR(-/-) mice. Although total lung RelB expression was increased by cigarette smoke, nuclear RelB was significantly lower in subchronically exposed AhR(-/-) mice. Inhibition of AhR activity by CH-223191 in endothelial cells potentiated ICAM-1 expression and prevented RelB nuclear translocation but had no effect on neutrophil adhesion. These data support that genetic absence of the AhR contributes to heightened pulmonary neutrophilia in response to ongoing cigarette smoke exposure. Interindividual variations in AhR expression may enhance the susceptibility to cigarette smoke-induced diseases. PMID:24752502

de Souza, Angela Rico; Zago, Michela; Eidelman, David H; Hamid, Qutayba; Baglole, Carolyn J

2014-07-01

115

Small GTPase Rho signaling is involved in {beta}1 integrin-mediated up-regulation of intercellular adhesion molecule 1 and receptor activator of nuclear factor {kappa}B ligand on osteoblasts and osteoclast maturation  

SciTech Connect

We assessed the characteristics of human osteoblasts, focusing on small GTPase Rho signaling. {beta}1 Integrin were highly expressed on osteoblasts. Engagement of {beta}1 integrins by type I collagen augmented expression of intercellular adhesion molecule 1 (ICAM-1) and receptor activator of nuclear factor {kappa}B ligand (RANKL) on osteoblasts. Rho was activated by {beta}1 stimulation in osteoblasts. {beta}1 Integrin-induced up-regulation of ICAM-1 and RANKL was inhibited by transfection with adenoviruses encoding C3 transferase or pretreated with Y-27632, specific Rho and Rho-kinase inhibitors. Engagement of {beta}1 integrin on osteoblasts induced formation of tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells (MNC) in a coculture system of osteoblasts and peripheral monocytes, but this action was completely abrogated by transfection of C3 transferase. Our results indicate the direct involvement of Rho-mediated signaling in {beta}1 integrin-induced up-regulation of ICAM-1 and RANKL and RANKL-dependent osteoclast maturation. Thus, Rho-mediated signaling in osteoblasts seems to introduce major biases to bone resorption.

Hirai, Fumihiko [First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu 807-8555 (Japan); Nakayamada, Shingo [First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu 807-8555 (Japan); Okada, Yosuke [First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu 807-8555 (Japan); Saito, Kazuyoshi [First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu 807-8555 (Japan); Kurose, Hitoshi [Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 (Japan); Mogami, Akira [Pharmaceuticals Research Unit Research and Development Division Mitsubishi Pharma Corporation, Kamoshida-cho, Aoba-ku, Yokohama 227-0033 (Japan); Tanaka, Yoshiya [First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu 807-8555 (Japan)]. E-mail: tanaka@med.uoeh-u.ac.jp

2007-04-27

116

Ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular trafficking of proteins and induces accumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum  

PubMed Central

Ursolic acid (3?-hydroxy-urs-12-en-28-oic acid) is a natural pentacyclic triterpenoid that is present in many plants, including medicinal herbs, and foods. Ursolic acid was initially identified as an inhibitor of the expression of intercellular adhesion molecule-1 (ICAM-1) in response to interleukin-1? (IL-1?). We report here a novel biological activity: ursolic acid inhibits intracellular trafficking of proteins. Ursolic acid markedly inhibited the IL-1?-induced cell-surface ICAM-1 expression in human cancer cell lines and human umbilical vein endothelial cells. By contrast, ursolic acid exerted weak inhibitory effects on the IL-1?-induced ICAM-1 expression at the protein level. Surprisingly, we found that ursolic acid decreased the apparent molecular weight of ICAM-1 and altered the structures of N-linked oligosaccharides bound to ICAM-1. Ursolic acid induced the accumulation of ICAM-1 in the endoplasmic reticulum, which was linked mainly to high-mannose-type glycans. Moreover, in ursolic-acid-treated cells, the Golgi apparatus was fragmented into pieces and distributed over the cells. Thus, our results reveal that ursolic acid inhibits intracellular trafficking of proteins and induces the accumulation of ICAM-1 linked to high-mannose-type glycans in the endoplasmic reticulum. PMID:24649404

Mitsuda, Satoshi; Yokomichi, Tomonobu; Yokoigawa, Junpei; Kataoka, Takao

2014-01-01

117

Ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular trafficking of proteins and induces accumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum.  

PubMed

Ursolic acid (3?-hydroxy-urs-12-en-28-oic acid) is a natural pentacyclic triterpenoid that is present in many plants, including medicinal herbs, and foods. Ursolic acid was initially identified as an inhibitor of the expression of intercellular adhesion molecule-1 (ICAM-1) in response to interleukin-1? (IL-1?). We report here a novel biological activity: ursolic acid inhibits intracellular trafficking of proteins. Ursolic acid markedly inhibited the IL-1?-induced cell-surface ICAM-1 expression in human cancer cell lines and human umbilical vein endothelial cells. By contrast, ursolic acid exerted weak inhibitory effects on the IL-1?-induced ICAM-1 expression at the protein level. Surprisingly, we found that ursolic acid decreased the apparent molecular weight of ICAM-1 and altered the structures of N-linked oligosaccharides bound to ICAM-1. Ursolic acid induced the accumulation of ICAM-1 in the endoplasmic reticulum, which was linked mainly to high-mannose-type glycans. Moreover, in ursolic-acid-treated cells, the Golgi apparatus was fragmented into pieces and distributed over the cells. Thus, our results reveal that ursolic acid inhibits intracellular trafficking of proteins and induces the accumulation of ICAM-1 linked to high-mannose-type glycans in the endoplasmic reticulum. PMID:24649404

Mitsuda, Satoshi; Yokomichi, Tomonobu; Yokoigawa, Junpei; Kataoka, Takao

2014-01-01

118

CD45 mAb induces cell adhesion in peripheral blood mononuclear cells via lymphocyte function-associated antigen-1 (LFA-1) and intercellular cell adhesion molecule 1 (ICAM-1).  

PubMed

A novel cell aggregation-inducing characteristic of the leukocyte common antigen, CD45, is described and its underlying molecular mechanisms investigated. Formation of strong cell clusters was consistently observed in human PBMCs after crosslinking CD45 molecules with antibodies, directed to epitopes common for all CD45 isoforms (e.g., mAb ROS220 or NIH45-2) or the CD45RA (e.g., mAb Alb11) or the CD45RO isoform (e.g., mAb UCHL1). This phenomenon was not seen after PBMC treatment with CD45RA mAb HB11 or CD2 mAb 39C1.5. Identical to phorbol 12-myristate 13-acetate (PMA)-induced clustering, CD45-mediated aggregation was also suppressed by EDTA, by cytochalasin B, and by incubation at 4 degrees C, all characteristics of adhesion mediated by integrins. The involvement of LFA-1 and ICAM-1 in CD45-mediated adhesion was supported by the observation that CD11a (LFA-1 alpha) mAb R7.1, CD18 (LFA-1 beta) mAb R3.3, and CD54 (ICAM-1) mAb R6.1 or RR/l all strongly inhibited CD45- and PMA-induced aggregation. Interestingly, highly pure T lymphocytes did not aggregate in response to CD45 mAb, but did after PMA treatment. These results indicate that triggering human PBMCs via CD45 can cause strong cell aggregation, largely through LFA-1/ICAM-1 interactions. Our findings support an important role of the CD45 antigen in signal transduction and intercellular interaction in human PBMCs. PMID:8096435

Lorenz, H M; Harrer, T; Lagoo, A S; Baur, A; Eger, G; Kalden, J R

1993-03-01

119

Time course of endothelial damage in septic shock: prediction of outcome  

Microsoft Academic Search

INTRODUCTION: Endothelial damage accounts greatly for the high mortality in septic shock. Higher expression of mediators (IL-6, IL-8, soluble intercellular adhesion molecule 1 [sICAM-1], soluble endothelial-linked adhesion molecule 1 [sELAM-1]) have been described for non-survivors in comparison with survivors. We investigated the predictive value of the mediators IL-6, IL-8, sELAM-1 and sICAM-1 and their time course in intensive care unit

Ortrud Vargas Hein; Klaudia Misterek; Jan-Peer Tessmann; Vera van Dossow; Michael Krimphove; Claudia Spies

2005-01-01

120

Aldosterone stimulates nuclear factor-kappa B activity and transcription of intercellular adhesion molecule-1 and connective tissue growth factor in rat mesangial cells via serum- and glucocorticoid-inducible protein kinase-1.  

PubMed

Several clinical and experimental data support the hypothesis that aldosterone contributes to the progression of renal injury. To determine the signaling pathway of aldosterone in relation to fibrosis and inflammation in mesangial cells, we investigated the effects of aldosterone on expression and activation of serum- and glucocorticoid-inducible protein kinase-1 (SGK1), the activation of nuclear factor-kappa B (NF-?B activation, and the expressions of intercellular adhesion molecule-1 (ICAM-1) and connective tissue growth factor (CTGF). Aldosterone stimulated SGK1 expression, phosphorylation (Ser-256), and kinase activity. The increments of phosphorylation and expression of SGK1 induced by aldosterone were inhibited by mineralocorticoid receptor (MR) inhibitor (eplerenone). Aldosterone stimulated NF-?B activity measured by NF-?B responsive elements, luciferase assay, and the levels of inhibitor of kappa B (I?B) phosphorylation. This aldosterone-induced activation of NF-?B was inhibited by the transfection of dominant-negative SGK1. Furthermore, aldosterone augmented the promoter activities and protein expressions of ICAM-1 and CTGF. The effects of aldosterone on ICAM-1 and CTGF promoter activities and protein expressions were inhibited by the transfection of dominant-negative SGK1 and dominant-negative I?B?. We also found that the MR antagonist significantly ameliorated the glomerular injury and enhancements in SGK1, ICAM-1, and CTGF expressions induced by 1% sodium chloride and aldosterone in vivo. In conclusion, our findings suggest that aldosterone stimulates ICAM-1 and CTGF transcription via activation of SGK1 and NF-?B, which may be involved in the progression of aldosterone-induced mesangial fibrosis and inflammation. MR antagonists may serve as useful therapeutic targets for the treatment of glomerular inflammatory disease. PMID:22042038

Terada, Yoshio; Ueda, Satoko; Hamada, Kazu; Shimamura, Yoshiko; Ogata, Koji; Inoue, Kosuke; Taniguchi, Yoshinori; Kagawa, Toru; Horino, Taro; Takao, Toshihiro

2012-02-01

121

Phorbol myristate acetate-activated keratinocytes stimulate proliferation of resting peripheral blood mononuclear lymphocytes via a MHC-independent, but protein kinase C- and intercellular adhesion molecule-1-dependent, mechanism.  

PubMed

Recent attention has focused on the role keratinocytes (KC) may play in the induction of T cell-mediated inflammatory responses in skin, particularly because KC, when activated by immunologic stimuli, express MHC class II Ag and secrete immunomodulatory cytokines. We tested the capacity of normal human KC that were stimulated with PMA to induce PBMC proliferation. PMA-treated, but not untreated, KC induced proliferation of allogeneic as well as autologous PBMC; in addition, when purified CD4+ or CD8+ T cells were used as responders, each subset proliferated. PBMC proliferation was not due to direct action of PMA on PBMC, nor to contamination of KC cultures with Langerhans cells (LC) or dermal APC. Pretreatment with different protein kinase C inhibitors abrogated the capacity of PMA-stimulated KC to induce proliferation. Paraformaldehyde-fixed PMA-KC stimulated PBMC proliferation, whereas supernatants from PMA-treated KC failed to do so, indicating that a membrane-associated activity on PMA-KC contributes to the induction of PBMC proliferation. PMA induced intercellular adhesion molecule-1 (ICAM-1) expression on KC; furthermore, mAb against ICAM-1 or against its ligand lymphocyte function-associated Ag (LFA-1) (CD11a/CD18) significantly, but incompletely, reduced the stimulatory capacity of PMA-treated KC, indicating that ICAM-1/LFA-1 interaction contributed to PBMC proliferation. IFN-gamma or TNF-alpha also induced ICAM-1 on KC, but these KC failed to stimulate proliferation, suggesting that PMA induces additional signals from KC, which act in concert with ICAM-1 to promote proliferation. Finally, mAb against HLA-ABC or HLA-DR did not inhibit proliferation. We conclude that PMA can activate KC to stimulate T cell proliferation in a MHC-independent fashion. This activation is mediated by protein kinase C and in part by the induction of ICAM-1 expression on KC. PMID:1670943

Simon, J C; Cruz, P D; Bergstresser, P R; Davis, L S; Tigelaar, R E

1991-01-15

122

Luteolin protects against vascular inflammation in mice and TNF-alpha-induced monocyte adhesion to endothelial cells via suppressing I?B?/NF-?B signaling pathway.  

PubMed

Vascular inflammation plays a significant role in the pathogenesis of atherosclerosis. Luteolin, a naturally occurring flavonoid present in many medicinal plants and some commonly consumed fruits and vegetables, has received wide attention for its potential to improve vascular function in vitro. However, its effect in vivo and the molecular mechanism of luteolin at physiological concentrations remain unclear. Here, we report that luteolin as low as 0.5?M significantly inhibited tumor necrosis factor (TNF)-?-induced adhesion of monocytes to human EA.hy 926 endothelial cells, a key event in triggering vascular inflammation. Luteolin potently suppressed TNF-?-induced expression of the chemokine monocyte chemotactic protein-1 (MCP-1) and adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), key mediators involved in enhancing endothelial cell-monocyte interaction. Furthermore, luteolin inhibited TNF-?-induced nuclear factor (NF)-?B transcriptional activity, I?B? degradation, expression of I?B kinase ? and subsequent NF-?B p65 nuclear translocation in endothelial cells, suggesting that luteolin can inhibit inflammation by suppressing NF-?B signaling. In an animal study, C57BL/6 mice were fed a diet containing 0% or 0.6% luteolin for 3 weeks, and luteolin supplementation greatly suppressed TNF-?-induced increase in circulating levels of MCP-1/JE, CXCL1/KC and sICAM-1 in C57BL/6 mice. Consistently, dietary intake of luteolin significantly reduced TNF-?-stimulated adhesion of monocytes to aortic endothelial cells ex vivo. Histology shows that luteolin treatment prevented the eruption of endothelial lining in the intima layer of the aorta and preserved elastin fibers' delicate organization as shown by Verhoeff-Van Gieson staining. Immunohistochemistry studies further show that luteolin treatment also reduced VCAM-1 and monocyte-derived F4/80-positive macrophages in the aorta of TNF-?-treated mice. In conclusion, luteolin protects against TNF-?-induced vascular inflammation in both in vitro and in vivo models. This anti-inflammatory effect of luteolin may be mediated via inhibition of the NF-?B-mediated pathway. PMID:25577468

Jia, Zhenquan; Nallasamy, Palanisamy; Liu, Dongmin; Shah, Halley; Li, Jason Z; Chitrakar, Rojin; Si, Hongwei; McCormick, John; Zhu, Hong; Zhen, Wei; Li, Yunbo

2015-03-01

123

Intercellular adhesion molecule-1/LFA-1 cross talk is a proximate mediator capable of disrupting immune integration and tolerance mechanism at the feto-maternal interface in murine pregnancies.  

PubMed

Our understanding why a woman's immune system does not reject her histoincompatible fetus is still very limited. Distinct insights into the mechanisms involved in pregnancy maintenance may help us to prevent pregnancy complications, e.g., miscarriages or pre-eclampsia. Immune integration and tolerance at the feto-maternal interface appear to be indispensable for successful pregnancy maintenance. Little is known about the cross talk between ICAM-1, expressed on epithelium, endothelium, and APC, and its ligand, LFA-1, at the feto-maternal interface. However, based on the role of ICAM-1/LFA-1 in allograft acceptance or rejection upon transplantation, adhesion molecules are likely to interfere with successful pregnancy outcome. In this study, we tested the hypothesis that ICAM-1/LFA-1 pathways may be involved in pregnancy rejection in murine models. By blocking ICAM-1/LFA-1-mediated intercellular adhesion events, we show that fetal immune acceptance is restored in challenged pregnancies (e.g., upon exposure to sound stress), and adoptive transfer of LFA-1 cells into pregnant mice induces rejection only in abortion-prone mouse models. ICAM-1/LFA-1 cross talk leads to increased recruitment of proinflammatory cells to the implantation site, promotes dendritic cell maturation in the decidua, and subsequently induces additional local Th1 polarization via mature dendritic cells. Furthermore, our observations clearly point out that mechanisms of fetal tolerance, e.g., indoleamine 2,3-dioxygenase expression, presence of CD4+CD25bright regulatory T cells, and synthesis of asymmetric Abs, are ICAM-1/LFA-1 dependent. Hence, our data shed light on a hierarchical network of immune integration at the feto-maternal interface, in which ICAM-1/LFA-1 cross talk is clearly a proximate mediator capable of disrupting successful pregnancy maintenance. PMID:15699108

Blois, Sandra; Tometten, Mareike; Kandil, Judith; Hagen, Evelin; Klapp, Burghard F; Margni, Ricardo A; Arck, Petra C

2005-02-15

124

Eosinophils Adhere to Vascular Cell Adhesion Molecule-1 via Podosomes  

E-print Network

; negative for gelsolin; and associated with microfilaments. Phorbol myristate acetate or tumor necrosis in tumor cells are associated with extracellularmatrix degradation, we analyzedthe VCAM-1 layer. VCAM-1, BSA; cluster of differentiation, CD; Chinese hamster ovary, CHO; fluorescence- activated cell sorting

Mosher, Deane F.

125

Reduction of the ST6 ?-galactosamide ?-2,6-sialyltransferase 1 (ST6GAL1)-catalyzed sialylation of nectin-like molecule 2/cell adhesion molecule 1 and enhancement of ErbB2/ErbB3 signaling by microRNA-199a.  

PubMed

Nectin-like molecule 2 (Necl-2)/cell adhesion molecule 1 (CADM1) is shown to be down-regulated by the promoter hypermethylation and/or loss of heterozygosity at chromosome 11q23.2 in many types of cancers, including lung and breast cancers, and is proposed to serve as a tumor suppressor. However, the incidence of these epigenetic and genetic abnormalities of Necl-2 is 30-60% in these cancers, and other mechanisms for the suppression of Necl-2 are presumed to be present. We previously showed that Necl-2 interacts in cis with ErbB3 and suppresses the heregulin (HRG)-induced ErbB2/ErbB3 signaling for cell movement and death. We studied here the relationship between Necl-2 and microRNA-199a (miR-199a) that is up-regulated or down-regulated in a variety of cancers. miR-199a did not directly target the Necl-2 mRNA or affect its mRNA level in human lung cancer A549 cells and human embryonic kidney HEK293 cells. Necl-2 was at least sialylated by the sialyltransferase ST6 ?-galactosamide ?-2,6-sialyltransferase 1 (ST6GAL1). miR-199a targeted ST6GAL1 and reduced both the sialylation and the protein level of Necl-2. In addition, miR-199a enhanced the HRG-induced ErbB2/ErbB3 signaling. These results indicate that the suppressive role of Necl-2 in the HRG-induced ErbB2/ErbB3 signaling is regulated by miR-199a at least through the reduction of the ST6GAL1-catalyzed sialylation of Necl-2 and/or through the reduction of the protein level of Necl-2 presumably by the protein degradation. PMID:23504322

Minami, Akihiro; Shimono, Yohei; Mizutani, Kiyohito; Nobutani, Kentaro; Momose, Kenji; Azuma, Takeshi; Takai, Yoshimi

2013-04-26

126

Brucella abortus as a potential vaccine candidate: induction of interleukin-12 secretion and enhanced B7.1 and B7.2 and intercellular adhesion molecule 1 surface expression in elutriated human monocytes stimulated by heat-inactivated B. abortus.  

PubMed Central

Development of a vaccine which is capable of generating a strong cellular immune response associated with gamma interferon (IFN-gamma) production and cytotoxic T-cell development requires that the immunogen be capable of inducing the secretion of interleukin-12 (IL-12), which is a pivotal factor for the differentiation of Th1 or Tc1 cells. We have previously shown that the heat-inactivated gram-negative bacterium Brucella abortus can induce IFN-gamma secretion by T cells. In the present study, we demonstrate that B. abortus and lipopolysaccharide (LPS) from B. abortus can induce IL-12 p40 mRNA expression and protein secretion by human elutriated monocytes (99% pure). p40 mRNA was detected within 4 h, and p40 protein could be measured at 24 h. This induction was abrogated by anti-CD14 monoclonal antibody, suggesting that monocytes recognize B. abortus via their receptor for LPS. The biological activity of IL-12 secreted by B. abortus-stimulated monocytes was demonstrated by its ability to upregulate IFN-gamma mRNA expression in T cells separated from monocytes and B. abortus by a transwell membrane. The B. abortus-induced IL-12 also enhanced NK cytolytic activity against K562 target cells. B. abortus was shown to rapidly increase the expression of the costimulatory molecules B7.1 and B7.2 and intercellular adhesion molecule 1 on human monocytes. Together, these data indicate that B. abortus can directly activate human monocytes and provide the cytokine milieu which would direct the immune response towards Th1-Tc1 differentiation. PMID:8757841

Zaitseva, M; Golding, H; Manischewitz, J; Webb, D; Golding, B

1996-01-01

127

Reduction of the ST6 ?-Galactosamide ?-2,6-Sialyltransferase 1 (ST6GAL1)-catalyzed Sialylation of Nectin-like Molecule 2/Cell Adhesion Molecule 1 and Enhancement of ErbB2/ErbB3 Signaling by MicroRNA-199a*  

PubMed Central

Nectin-like molecule 2 (Necl-2)/cell adhesion molecule 1 (CADM1) is shown to be down-regulated by the promoter hypermethylation and/or loss of heterozygosity at chromosome 11q23.2 in many types of cancers, including lung and breast cancers, and is proposed to serve as a tumor suppressor. However, the incidence of these epigenetic and genetic abnormalities of Necl-2 is 30–60% in these cancers, and other mechanisms for the suppression of Necl-2 are presumed to be present. We previously showed that Necl-2 interacts in cis with ErbB3 and suppresses the heregulin (HRG)-induced ErbB2/ErbB3 signaling for cell movement and death. We studied here the relationship between Necl-2 and microRNA-199a (miR-199a) that is up-regulated or down-regulated in a variety of cancers. miR-199a did not directly target the Necl-2 mRNA or affect its mRNA level in human lung cancer A549 cells and human embryonic kidney HEK293 cells. Necl-2 was at least sialylated by the sialyltransferase ST6 ?-galactosamide ?-2,6-sialyltransferase 1 (ST6GAL1). miR-199a targeted ST6GAL1 and reduced both the sialylation and the protein level of Necl-2. In addition, miR-199a enhanced the HRG-induced ErbB2/ErbB3 signaling. These results indicate that the suppressive role of Necl-2 in the HRG-induced ErbB2/ErbB3 signaling is regulated by miR-199a at least through the reduction of the ST6GAL1-catalyzed sialylation of Necl-2 and/or through the reduction of the protein level of Necl-2 presumably by the protein degradation. PMID:23504322

Minami, Akihiro; Shimono, Yohei; Mizutani, Kiyohito; Nobutani, Kentaro; Momose, Kenji; Azuma, Takeshi; Takai, Yoshimi

2013-01-01

128

Reduction of adhesion molecule production and alteration of eNOS and endothelin-1 mRNA expression in endothelium by Euphorbia hirta L. through its beneficial ?-amyrin molecule.  

PubMed

The inflammatory reaction in large blood vessels involves up-regulation of vascular adhesion molecules such as endothelial cell selectin (E-selectin), soluble vascular cell adhesion molecule (sVCAM)-1, and soluble intercellular adhesion molecule (sICAM)-1. These vascular dysfunctions are associated with the development of atherosclerosis. ?-Amyrin, an active component of Euphorbia hirta L., has potent anti-inflammatory effects. So far, its preventive effects against the expression of inflammatory mediator-induced adhesion molecules have not been investigated. Endothelial cells (SVEC4-10 cell line) were treated with 50% RAW conditioned media (i.e., normal SVEC4-10 culture media contains 50% of lipopolysaccharide-activated macrophage culture media) without or with ?-amyrin (0.6 and 0.3 µM). The production levels of E-selectin, sICAM-1, and sVCAM-1 in the SVEC4-10 cells were measured with ELISA assay kits. Under the same treatment conditions, expression of endothelin (ET)-1 and endothelial type of NO synthase (eNOS) mRNA were analyzed by RT-PCR and agarose gel. With ?-amyrin, the 50% RAW conditioned media-induced E-selectin, sICAM-1, and sVCAM-1 levels as well as ET-1 gene expression were all suppressed. ?-Amyrin treatment also restored the 50% RAW conditioned media-suppressed eNOS mRNA expression. These data indicate that ?-amyrin is potentially useful in preventing chronic inflammation-related vascular diseases. PMID:25045892

Shih, Mei Fen; Cherng, Jong Yuh

2014-01-01

129

Combined measurement of soluble and cellular ICAM-1 among children with Plasmodium falciparum malaria in Uganda  

PubMed Central

Background Intercellular adhesion molecule-1 (ICAM-1) is a cytoadhesion molecule implicated in the pathogenesis of Plasmodium falciparum malaria. Elevated levels of soluble ICAM-1 (sICAM-1) have previously been reported with increased malaria disease severity. However, studies have not yet examined both sICAM-1 concentrations and monocyte ICAM-1 expression in the same cohort of patients. To better understand the relationship of soluble and cellular ICAM-1 measurements in malaria, both monocyte ICAM-1 expression and sICAM-1 concentration were measured in children with P. falciparum infection exhibiting a spectrum of clinical severity. Methods Samples were analysed from 160 children, aged 0.5 to 10.8 years, with documented P. falciparum malaria in Kampala, Uganda. The patients belonged to one of three pre-study defined groups: uncomplicated malaria (UM), severe non-fatal malaria (SM-s), and fatal malaria (SM-f). Subset analysis was done on those with cerebral malaria (CM) or severe malaria anaemia (SMA). Monocyte ICAM-1 was measured by flow cytometry. sICAM-1 was measured by enzyme immunoassay. Results Both sICAM-1 and monocyte cell-surface ICAM-1 followed a log-normal distribution. Median sICAM-1 concentrations increased with greater severity-of-illness: 279 ng/mL (UM), 462 ng/mL (SM-s), and 586 ng/mL (SM-f), p < 0.0001. sICAM-1 levels were not statistically different among children with CM compared to SMA. Monocyte ICAM-1 expression was significantly higher in cases of UM compared with SM-s or SM-f (p < 0.001) and was higher among the subset of patients with CM compared with SMA, p < 0.0014. The combination of sICAM-1 and cellular ICAM-1 identified distinct categories of patients (UM with low sICAM-1 and higher monocyte ICAM-1, CM with both sICAM-1 and monocyte ICAM-1 high, and SMA with sICAM-1 high but monocyte ICAM-1 low). Conclusion In this cohort of children with P. falciparum malaria, sICAM-1 levels were associated with severity-of-illness. Patients with UM had higher monocyte ICAM-1 expression consistent with a role for monocyte ICAM-1 in immune clearance during non-severe malaria. Among the subsets of patients with either SMA or CM, monocyte ICAM-1 levels were higher in CM, consistent with the role of ICAM-1 as a marker of cytoadhesion. Categories of disease in pediatric malaria may exhibit specific combinations of soluble and cellular ICAM-1 expression. PMID:20712868

2010-01-01

130

Circulating soluble ICAM-1 and subclinical atherosclerosis: The Coronary Artery Risk Development in Young Adults (CARDIA) Study  

PubMed Central

Background Soluble intercellular adhesion molecule-1 (sICAM-1) is associated with endothelial dysfunction and clinical cardiovascular disease. We investigated the relationship of subclinical atherosclerosis with sICAM-1 concentration. Methods sICAM-1 concentration was assayed at year 15 of the Coronary Artery Risk Development in Young Adults (CARDIA) Study (black and white men and women, average age 40 years). We assessed progression of coronary artery calcification through year 20 (CAC, n=2378), and both carotid artery stenosis (n=2432) and intima media thickness at year 20 (IMT, n = 2240). Results Median sICAM-1 was 145.9 ng/ml. Among a subgroup with advanced atherosclerotic plaque (either CAC or stenosis), IMT was 0.010 (95% confidence interval (CI) 0.003–0.017 mm) higher per standard deviation of sICAM-1 (44 ng/ml) in a model adjusted for age, race, sex, clinic, smoking, exercise, body size, education, blood pressure, antihypertensive medication, plasma lipids, and cholesterol lowering medication. With the same adjustment, the odds ratios (OR) for the presence of year 20 carotid artery stenosis per SD of sICAM-1 was 1.12 (CI 1.01–1.25, p<0.04), while for occurrence of CAC progression the OR was 1.16 (CI 1.04–1.31, p<0.01). The associations with CAC and carotid stenosis were strongest in the top 20th of the sICAM-1 distribution. Conclusion sICAM-1 concentration may be an early biomarker that indicates changes in the artery wall that accompany atherosclerosis, as well as the presence of advanced plaque in the coronary and carotid arteries. This finding holds in people with low total burden of atherosclerosis, decades prior to the development of clinical CVD. PMID:22179741

Gross, Myron D.; Bielinski, Suzette J.; Suarez-Lopez, Jose R.; Reiner, Alex P.; Bailey, Kent; Thyagarajan, Bharat; Carr, J. Jeffrey; Duprez, Daniel A.; Jacobs, David R.

2013-01-01

131

Association of ICAM-1 K469E polymorphism with neurocysticercosis.  

PubMed

Neurocysticercosis (NCC), a central nervous system (CNS) disease is caused by the larval stage of Taenia solium. The disease is heterogeneous in clinical presentation; some infected individuals develop symptoms and others may remain symptom free. Impaired blood brain barrier allows recruitment of immune cells in the CNS during infection and soluble intercellular adhesion molecule-1 (sICAM-1) plays an important role in the recruitment of immune cells. We studied ICAM-1 K469E polymorphism among symptomatic and asymptomatic NCC patients. The study revealed that individuals with variant (EE) genotype were more susceptible to symptomatic NCC and also had an elevated level of sICAM-1. PMID:25128351

Singh, Amrita; Singh, Aloukick K; Singh, Satyendra K; Paliwal, Vimal K; Gupta, Rakesh K; Prasad, Kashi N

2014-11-15

132

Soluble adhesion molecules in serum throughout the menstrual cycle  

Microsoft Academic Search

BACKGROUND: The female reproductive and immune systems are integrally linked with respect to shared cellular and molecular mediators. Cell adhesion molecules (CAMs) involved in leukocyte-endothelial interactions, e.g. intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-selectin, are regulated by sex steroids when expressed by cultured endothelium, while uterine and ovarian CAM expression appears to be cyclically or gonadotrophin-regulated.

Nigel Bonello; Robert J. Norman

2002-01-01

133

Comparative effects of quercetin and its predominant human metabolites on adhesion molecule expression in activated human vascular endothelial cells  

Microsoft Academic Search

Adhesion of circulating monocytes to vascular endothelial cells, a critical step in both inflammation and atherosclerosis, is mediated by cross-linkage of adhesion molecules expressed on the surface of both cell types. Dietary flavonoids have been shown to have anti-inflammatory properties, decreasing the expression of cell adhesion molecules, such as vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) on

Sandra Tribolo; Federica Lodi; Carol Connor; Sunita Suri; Vincent G. Wilson; Moira A. Taylor; Paul W. Needs; Paul A. Kroon; David A. Hughes

2008-01-01

134

Association of Biomarkers of Inflammation with Dyslipidemia and Its Components among Mongolians in China  

PubMed Central

Objective This study aims to examine the association between inflammatory biomarkers and dyslipidemia and its components among Mongolians in China. Methods Data were obtained from 2544 Mongolians via standard questionnaires and blood samples in Inner Mongolia, China. High sensitivity C-reactive protein (hsCRP), soluble intercellular adhesion molecule-1 (sICAM-1) and soluble E-selectin (sE-selectin) as well as blood lipids were examined. Results Individuals with dyslipidemia had higher levels of hsCRP, sICAM-1 and sE-selectin than those without dyslipidemia (all P values<0.001). Compared to the lowest quartile of inflammatory biomarkers, individuals with the highest quartile were more likely to have dyslipidemia (odds ratio, 95% confidence interval: 3.215, 2.551–4.116 for hsCRP; 1.575, 1.253–1.980 for sICAM-1; 1.495, 1.193–1.873 for sE-selectin). Moreover, hsCRP was associated with all the components of dyslipidemia, whereas, sICAM-1 was not related to high density lipoprotein cholesterol (HDL-c) or triglycerides (TAG). Additionally, sE-selectin was just associated with TAG. Conclusion Our study indicated that elevated plasma levels of hsCRP, sICAM-1 and sE-selectin were positively and significantly associated with increased risk of dyslipidemia among Mongolians. However, the associations were not identical for different inflammatory biomarkers with the components of dyslipidemia. PMID:24558466

Tang, Lingyan; Peng, Hao; Xu, Tian; Wang, Aili; Wang, Guiyan; Tong, Weijun; Zhang, Yonghong

2014-01-01

135

Circulating soluble ICAM-1 levels shows linkage to ICAM gene cluster region on Chromosome 19: the NHLBI Family Heart Study Follow-up Examination  

PubMed Central

Atherogenesis is a chronic inflammatory process in which intercellular adhesion molecule 1 (ICAM-1) plays a critical role. Circulating soluble ICAM-1 (sICAM-1) is thought to be the result of cleavage of membrane-bound ICAM-1 and its concentration in serum/plasma has been shown to be heritable. Genome-wide linkage scans were conducted for quantitative trait loci influencing sICAM-1. Phenotype and genetic marker data were available for 2,617 white and 531 black individuals in the NHLBI Family Heart Study follow-up examination. Heritability for sICAM-1 was 0.39 in whites and 0.59 in blacks. Significant linkage was observed on chromosome 19 (LOD = 4.0 at 14 cM) in whites near the ICAM gene cluster that includes the structural gene for ICAM-1. The T-allele of ICAM-1 SNP rs5491 has been strongly associated with the specific sICAM-1 assay we used in our study. Through additional genotyping we were able to rule out rs5491 as the cause of the linkage finding. This study provides preliminary evidence linking genetic variation in the ICAM-1 structural gene to circulating sICAM-1 levels. PMID:18045607

Bielinski, Suzette J.; Pankow, James S.; Foster, Catherine Leiendecker; Miller, Michael B.; Hopkins, Paul N.; Eckfeldt, John H.; Hixson, Jim; Liu, Yongmei; Register, Tom; Myers, Richard H.; Arnett, Donna K.

2008-01-01

136

Puerarin Inhibits Adhesion Molecule Expression in TNF-?-Stimulated Human Endothelial Cells via Modulation of the Nuclear Factor ?B Pathway  

Microsoft Academic Search

Background: The isoflavone puerarin is the most abundant isoflavone-C-glucoside extracted from the root (radix puerariae) of the plant Pueraria lobata and possesses many biological activities. In this report, the ability of puerarin to modulate intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and endothelial leukocyte adhesion molecule 1 (E-selectin), and to induce changes in the nuclear factor

Wenzhi Hu; Qin Zhang; Xiangjun Yang; Yueying Wang; Lie Sun

2010-01-01

137

Toner Adhesion  

Microsoft Academic Search

The adhesion of toner particles charged by triboelectricity plays an important role in the electrophotographic process. In spite of the importance of this phenomenon to electrophotography, the physics of toner adhesion is not well understood. A literature survey of toner adhesion measurements reveals adhesion forces which are typically 5 to 50 times larger than the predictions of the electrostatic image

Dan A. Hays

1995-01-01

138

Alteration of soluble adhesion molecules during aging and their modulation by calorie restriction  

Microsoft Academic Search

To investigate the status of soluble adhesion molecules (sAMs) during aging, the present study determined protein levels of several major sAMs in serum samples obtained from rats at different ages. These sAMs include E-selectin, P-selectin, vascular cell adhesion molecule 1 (VCAM-1), and intercellular adhesion molecule 1 (ICAM-1). Fischer 344 rats, ages 6, 12, 18, and 24 months, fed ad libitum

Yani Zou; Kyung Jin Jung; Jung Won Kim; Byung Pal Yu; Hae Young Chung

2003-01-01

139

Dark chocolate consumption improves leukocyte adhesion factors and vascular function in overweight men.  

PubMed

Flavanol-enriched chocolate consumption increases endothelium-dependent vasodilation. Most research so far has focused on flow-mediated dilation (FMD) only; the effects on other factors relevant to endothelial health, such as inflammation and leukocyte adhesion, have hardly been addressed. We investigated whether consumption of regular dark chocolate also affects other markers of endothelial health, and whether chocolate enrichment with flavanols has additional benefits. In a randomized double-blind crossover study, the effects of acute and of 4 wk daily consumption of high flavanol chocolate (HFC) and normal flavanol chocolate (NFC) on FMD, augmentation index (AIX), leukocyte count, plasma cytokines, and leukocyte cell surface molecules in overweight men (age 45-70 yr) were investigated. Sensory profiles and motivation scores to eat chocolate were also collected. Findings showed that a 4 wk chocolate intake increased FMD by 1%, which was paralleled by a decreased AIX of 1%, decreased leukocyte cell count, decreased plasma sICAM1 and sICAM3, and decreased leukocyte adhesion marker expression (P<0.05 for time effect), with no difference between HFC and NFC consumption. Flavanol enrichment did affect taste and negatively affected motivation to consume chocolate. This study provides new insights on how chocolate affects endothelial health by demonstrating that chocolate consumption, besides improving vascular function, also lowers the adherence capacity of leukocytes in the circulation. PMID:24302679

Esser, Diederik; Mars, Monica; Oosterink, Els; Stalmach, Angelique; Müller, Michael; Afman, Lydia A

2014-03-01

140

Circulating adhesion molecules after short-term exposure to particulate matter among welders  

PubMed Central

Background Studies from several countries indicate that welders experience increased risk of mortality and morbidity from ischaemic heart disease. Although the underlying mechanisms are unclear, vascular responses to particulate matter contained in welding fumes may play a role. To investigate this, we studied the acute effects of welding fume exposure on the endothelial component of vascular function, as measured by circulating adhesion molecules involved in leukocyte adhesion (sICAM-1 and sVCAM-1) and coagulation (vWF). Methods A panel of 26 male welders was studied repeatedly across a 6 h work-shift on a high exposure welding day and/or a low exposure non-welding day. Personal PM2.5 exposure was measured throughout the work-shift. Blood samples were collected in the morning (baseline) prior to the exposure period, immediately after the exposure period, and the following morning. To account for the repeated measurements, we used linear mixed models to evaluate the effects of welding (binary) and PM2.5 (continuous) exposure on each blood marker, adjusting for baseline blood marker concentration, smoking, age and time of day. Results Welding and PM2.5 exposure were significantly associated with a decrease in sVCAM-1 in the afternoon and the following morning and an increase in vWF in the afternoon. Conclusions The data suggest that welding and short-term occupational exposure to PM2.5 may acutely affect the endothelial component of vascular function. PMID:19736177

Fang, S C; Eisen, E A; Cavallari, J M; Mittleman, M A; Christiani, D C

2011-01-01

141

Application of X-ray microanalysis to study of the expression of endothelial adhesion molecules on human umbilical vein endothelial cells in vitro  

Microsoft Academic Search

A semi-quantitative procedure is described, which allows the evaluation of expression levels of endothelial adhesion molecules on cultured human umbilical vein endothelial cells (HUVEC) using energy dispersive X-ray microanalysis (EDX). As a model two adhesion molecules, E-selection (CD62E; ELAM-1\\/endothelial leukocyte adhesion molecule-1) and ICAM-1 (intercellular adhesion molecule-1; CD54), were localized by the use of the silver-enhancement colloidal gold method after

Janusz Tomczok; Wanda Sliwa-Tomczok; Christoph L. Klein; Fernando Bittinger; Charles J. Kirkpatrick

1994-01-01

142

Association of ophthalmic complications in patients with sulfur mustard induced mild ocular complications and serum soluble adhesion molecules: Sardasht-Iran Cohort Study.  

PubMed

The aim of this study was to evaluate possible association between ophthalmic complications in sulfur mustard (SM) exposed patients with mild ocular injuries and serum soluble adhesion molecules. Serum levels of sICAM-1, sL-selectin, sP-selectin and sE-selectin in 367 SM-exposed individuals with or without eye injuries were checked and compared with 128 unexposed controls. All participants underwent ocular examinations. Serum sICAM-1 level in SM exposed with blurred vision, was significantly (p=0.021) higher than in SM exposed with no blurred vision. Serum sL-selectin level was significantly (p=0.024) higher in SM exposed with photophobia than SM exposed with no photophobia. Serum P-selectin level in exposed without any slit lamp findings was significantly (p=0.003) lower than the matched control groups. Similar finding was seen in exposed group without ocular problem compared with the control groups. Serum sE-selectin level in exposed with normal ocular condition except for photophobia and blurred vision was significantly (p<0.05) higher than the matched controls. Serum E-selectin level in exposed with photophobia condition was significantly (p=0.047) higher than the control group with photophobia. In conclusion it seems that the changes in the E- and P-selectins is a regulatory mechanism for inhibition of SM induced ocular problems, although the local levels are more important and further investigations required in more severe ocular problems in SM exposed patients. PMID:23370300

Ghasemi, Hassan; Yaraee, Roya; Hassan, Zuhair Mohammad; Faghihzadeh, Soghrat; Soroush, Mohammad-Reza; Pourfarzam, Shahriar; Ebtekar, Massoumeh; Babaei, Mahmoud; Moaiedmohseni, Sakine; Naghizadeh, Mohammad-Mehdi; Askari, Nayere; Ghazanfari, Tooba

2013-11-01

143

Mouse Model of Transplant Arteriosclerosis Role of Intercellular Adhesion Molecule1  

Microsoft Academic Search

Transplant-accelerated arteriosclerosis in coronary arteries is the major limitation to long-term survival of patients with heart transplantation. The pathogenesis of this disease is not fully understood. Herein, we describe a simplified model of artery allografts in the mouse that allows us to take advantage of transgenic, knockout, or mutant animals. Common carotid arteries or aortic vessels were end-to-end allografted into

Hermann Dietrich; Yanhua Hu; Yiping Zou; Stephan Dirnhofer; Roman Kleindienst; Georg Wick; Qingbo Xu

144

Intercellular Adhesion Molecule-1 Guides Naive T Cell Differentiation and Regulatory T Cell Induction  

E-print Network

, each with a unique function in the immune response. In this Dissertation, we provide supporting evidence for the participation of costimulatory molecules in cell fate decisions. Using an in vitro differentiation system, we demonstrate that costimulation...

Williams, Kelli M.

2012-12-31

145

Intrauterine Adhesions  

MedlinePLUS

... involve the uterus. Other possible causes of adhesion formation are infections of the uterine lining (endometritis), removal of fibroids in the cavity of the uterus and endometrial ablation (a surgical ...

146

Monocyte cell adhesion induced by a human aminoacyl-tRNA synthetase-associated factor, p43: identification of the related adhesion molecules and signal pathways  

Microsoft Academic Search

An aminoacyl-tRNA synthetase-associ- ated factor, p43, was recently shown to be secreted to induce a proinflammatory response. Because a proinflammatory response involves the cell-cell ad- hesion between endothelial and immune cells, we first examined the mechanism of p43-induced cell- cell adhesion of myelomonocytic leukemia cells. Intercellular adhesion molecule-1 (ICAM-1) was up-regulated by p43 and mediated p43-induced cell-cell adhesion via the

Sang Gyu Park; Joong-Won Lee; Taeho Kim; Gyuyoup Kim; Young-Gyu Ko; Sunghoon Kim

147

Polyimide adhesives  

NASA Technical Reports Server (NTRS)

A process of preparing aromatic polyamide-acids for use as adhesives is described. An equimolar quantity of an aromatic dianhydride is added to a stirred solution of an aromatic diamine in a water or alcohol-miscible ether solvent to obtain a viscous polymer solution. The polymeric-acid intermediate polymer does not become insoluble but directly forms a smooth viscous polymer solution. These polyamic-acid polymers are converted, by heating in the range of 200-300 C and with pressure, to form polyimides with excellent adhesive properties.

Progar, D. J.; Bell, V. L.; Saintclair, T. L. (inventors)

1974-01-01

148

Relationship between serum estrogen levels and blood stasis syndrome in postmenopausal women with coronary heart disease  

PubMed Central

Objectives: To investigate the difference of serum estrogen, serum lipids and inflammatory factors levels in postmenopausal women with coronary heart blood stasis syndrome and non-blood stasis syndrome. Methods: Twenty five healthy postmenopausal women were selected as a healthy control group who were compared with 43 postmenopausal women with coronary heart disease (CHD) first visiting a doctor for the CHD. Among the postmenopausal women with CHD, There were 23 patients with blood stasis syndrome (BSS) and 20 patients with non-blood stasis syndrome (NBSS). The levels of plasma triglyceride (TG), total cholesterol (TC) were determined in blood samples taken after patients’ admission in Beijing Anzhen Hospital. The serum estradiol(E2) was measured by electrochemiluminescence assay and soluble intercellular adhesion molecule-1(sICAM-1) was measured by enzyme-linked immune sorbent assay (ELISA). Results: Compared with the healthy control group, the levels of TG and TC, sICAM-1 in coronary heart disease group were all significantly increased (P<0.05),but serum E2 were significantly decreased (P<0.05). The levels of E2 of patients with blood stasis syndrome (BSS) were decreased further (P>0.05), and there was an increasing trend of serum sICAM-1 levels (P>0.05). There were negative significant correlations between serum E2 levels and TC, sICAM-1 levels in patient with coronary heart disease. Conclusion: The estrogen level of menopausal women with coronary heart disease is lower than healthy menopausal women. With the low estrogen levels, postmenopausal women tend to have high levels of blood lipids and sICAM-1, which elucidates that the estrogen could regulate lipids and attenuate inflammatory response to play a protective role on blood vessels.

Liu, Xin; Guo, Chunyu; Ma, Xiaojuan; Tian, Rui; Zhang, Ying; Yin, Huijun

2015-01-01

149

Determining 2-integrin and ICAM-1 binding kinetics in tumor cells adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay*  

E-print Network

1 Determining 2-integrin and ICAM-1 binding kinetics in tumor cells adhesion to leukocytes-integrin to intercellular adhesion molecule 1 (ICAM-1). Here the adhesions between human WM9 metastatic forward and reverse rates of the 2-integrin-ICAM-1 bond between PMN-TC and PMN-EC pairs. This GDMAT assay

Dong, Cheng

150

ELAM1 Mediates Cell Adhesion by Recognition of a Carbohydrate Ligand, Sialyl-Le^x  

Microsoft Academic Search

Recruitment of neutrophils to sites of inflammation is mediated in part by endothelial leukocyte adhesion molecule-1 (ELAM-1), which is expressed on activated endothelial cells of the blood vessel walls. ELAM-1 is a member of the LEC-CAM or selectin family of adhesion molecules that contain a lectin motif thought to recognize carbohydrate ligands. In this report, cell adhesion by ELAM-1 is

M. Laurie Phillips; Edward Nudelman; Federico C. A. Gaeta; Mary Perez; Anil K. Singhal; Sen-Itiroh Hakomori; James C. Paulson

1990-01-01

151

Levels of soluble adhesion molecules in various clinical presentations of coronary atherosclerosis  

Microsoft Academic Search

Adhesion molecules play an important role in the development and course of coronary atherosclerosis. In this study, soluble forms of vascular cell adhesion molecule (VCAM-1) intercellular adhesion molecule-1 (ICAM-1), E-selectin and P-selectin were evaluated in patients with various clinical presentations of coronary atherosclerosis and compared them to those with angiographically documented normal coronary arteries. Venous plasma samples were collected from

Ümit Güray; A. Riza Erbay; Yesim Güray; M. Birhan Yilmaz; Asiye Ayca Boyac?; Hatice Sasmaz; Sule Korkmaz; Emine Kütük

2004-01-01

152

Some fundamentals of adhesion in synthetic adhesives  

Microsoft Academic Search

Various adhesion mechanisms that have been understood in the field of synthetic adhesives are described and these are linked with situations relevant to fouling issues. The review mainly deals with mechanical aspects of adhesion phenomena, with an emphasis on the role of the elasticity of the bodies, called substrata, attached by adhesive. The consequences of thin film geometry of the

Cyprien Gay

2003-01-01

153

Polyimide adhesives  

NASA Technical Reports Server (NTRS)

A process was developed for preparing aromatic polyamide acids for use as adhesives by reacting an aromatic dianhydride to an approximately equimolar amount of an aromatic diamine in a water or lower alkanol miscible ether solvent. The polyamide acids are converted to polyimides by heating to the temperature range of 200 - 300 C. The polyimides are thermally stable and insoluble in ethers and other organic solvents.

Progar, D. J.; Bell, V. L.; Stclair, T. L. (inventors)

1977-01-01

154

Serological Tests for Diagnosis and Staging of Hand–Arm Vibration Syndrome (HAVS)  

PubMed Central

The current gold standard for the diagnosis and staging of hand–arm vibration syndrome (HAVS) is the Stockholm workshop scale, which is subjective and relies on the patient’s recalling ability and honesty. Therefore, great potentials exist for diagnostic and staging errors. The purpose of this study is to determine if objective serum tests, such as levels of soluble thrombomodulin (sTM) and soluble intercellular adhesion molecule-1 (sICAM-1), may be used in the diagnosis and staging of HAVS. Twenty two nonsmokers were divided into a control group (n?=?11) and a vibration group (n?=?11). The control group included subjects without history of frequent vibrating tool use. The vibration group included construction workers with average vibrating tool use of 12.2 years. All were classified according to the Stockholm workshop scale (SN, sensorineural symptoms; V, vascular symptoms. SN0, no numbness; SN1, intermittent numbness; SN2, reduced sensory perception; SN3, reduced tactile discrimination; V0, no vasospasmic attacks; V1, intermittent vasospasm involving distal phalanges; V2, intermittent vasospasm extending to middle phalanges; V3, intermittent vasospasm extending to proximal phalanges; V4, skin atrophy/necrosis). All control subjects were SN0 V0. Seven out of 11 vibration subjects were SN1 V1, and 4 out of 11 were SN1 V2. A 10-cm3 sample of venous blood was collected from each subject. The sTM and sICAM-1 levels were determined by enzyme-linked immunosorbent assay. The mean plasma sTM levels were as follows: control group?=?2.93?±?0.47 ng/ml, and vibration group?=?3.61?±?0.24 ng/ml. The mean plasma sICAM-1 levels were as follows: control group?=?218.8?±?54.1 ng/ml, and vibration group?=?300.3?±?53.2 ng/ml. The sTM and sICAM-1 differences between control and vibration groups were statistically significant (p?

Yan, Ji-Geng; Zhang, Lin-Ling; Kaplan, Rachel E.; Riley, Danny A.; Matloub, Hani S.

2007-01-01

155

Fractalkine, a CX3C-chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP1  

Microsoft Academic Search

A newly identified CX3C-chemokine, fractalkine, expressed on activated endothelial cells plays an important role in leucocyte adhesion and migration. Co-immobilized fractalkine with fibronectin or intercellular adhesion molecule-1 enhanced adhesion of THP-1 cells, which express the fractalkine receptor (CX3CR1), compared with that observed for each alone. That adherence was fractalkine-dependent and was confirmed in blocking studies. However, soluble fractalkine induced little

Hisanori Umehara; Seiji Goda; Toshio Imai; Yutaka Nagano; Yasuhiro Minami; Yoshiya Tanaka; Toshiro Okazaki; Eda T Bloom; Naochika Domae

2001-01-01

156

The antioxidant effect of angiotensin II receptor blocker, losartan, in streptozotocin-induced diabetic rats.  

PubMed

We determined the effect of a short-term angiotensin II signaling blockade on vascular endothelial growth factor (VEGF), soluble intercellular adhesion molecule-1 (sICAM-1), nitric oxide (NO), and malondialdehyde (MDA) (index of lipid peroxidation) levels in the systemic circulation and on peroxynitrite generation and insulitis development in the streptozotocin (STZ) diabetic rats' pancreas. Diabetes was induced in Wistar rats by intraperitoneal STZ injection. Diabetic rats were treated for 1 week with losartan (20 mg/kg/body weight/day in the drinking water), and pancreas and blood were collected for histochemical, immunohistochemical, and biochemical studies. Diabetic rats showed greater VEGF, sICAM-1, NO, and MDA levels, a high score of insulitis, increased nitrotyrosine staining, and markedly reduced pancreatic insulin content when compared with controls. Losartan treatment suppressed the excessive NO and lipid peroxidation production systemically without restoring them to that of healthy subjects and reduced VEGF levels while leaving sICAM-1 levels unchanged. The insulitis score and nitrotyrosine staining were reduced, whereas the pancreatic islets and the beta-cell area were increased significantly in the treated group, indicating the reduction of inflammation and nitrosative stress and an early regeneration of beta-cell mass in the pancreas. Conclusively, in the STZ diabetic rat model, even a short-term losartan treatment improves oxidative and nitrosative stress systemically and locally, improving the islets' environment and accelerating beta-cell regeneration. PMID:20621034

Kamper, Maria; Tsimpoukidi, Olia; Chatzigeorgiou, Antonios; Lymberi, Maria; Kamper, Elli F

2010-07-01

157

Aqueous flare and inflammatory factors in macular edema with central retinal vein occlusion: a case series  

PubMed Central

Background The association of inflammatory factors and the aqueous flare value with macular edema in central retinal vein occlusion (CRVO) patients remains unclear. We investigated the relations between the aqueous flare value and vitreous levels of vascular endothelial growth factor (VEGF), soluble intercellular adhesion molecule-1 (sICAM-1), and interleukin-6 (IL-6) in patients with CRVO and macular edema or patients with idiopathic macular hole (MH). Methods In 38 patients who underwent unilateral vitrectomy (21 CRVO patients and 17 MH patients), vitreous samples were obtained during vitrectomy to measure VEGF, sICAM-1, and IL-6. Retinal ischemia was evaluated from capillary non-perfusion on fluorescein angiography, and the CRVO patients were classified into nonischemic or ischemic groups. Aqueous flare values were measured with a laser flare meter and macular edema was examined by optical coherence tomography. Results The median aqueous flare value increased significantly across the three groups (MH group?

2013-01-01

158

Iron sucrose accelerates early atherogenesis by increasing superoxide production and upregulating adhesion molecules in CKD.  

PubMed

High-dose intravenous iron supplementation is associated with adverse cardiovascular outcomes in patients with CKD, but the underlying mechanism is unknown. Our study investigated the causative role of iron sucrose in leukocyte-endothelium interactions, an index of early atherogenesis, and subsequent atherosclerosis in the mouse remnant kidney model. We found that expression levels of intracellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and adhesion of U937 cells increased in iron-treated human aortic endothelial cells through upregulated NADPH oxidase (NOx) and NF-?B signaling. We then measured mononuclear-endothelial adhesion and atherosclerotic lesions of the proximal aorta in male C57BL/6 mice with subtotal nephrectomy, male apolipoprotein E-deficient (ApoE(-/-)) mice with uninephrectomy, and sham-operated mice subjected to saline or parenteral iron loading. Iron sucrose significantly increased tissue superoxide production, expression of tissue cell adhesion molecules, and endothelial adhesiveness in mice with subtotal nephrectomy. Moreover, iron sucrose exacerbated atherosclerosis in the aorta of ApoE(-/-) mice with uninephrectomy. In patients with CKD, intravenous iron sucrose increased circulating mononuclear superoxide production, expression of soluble adhesion molecules, and mononuclear-endothelial adhesion compared with healthy subjects or untreated patients. In summary, iron sucrose aggravated endothelial dysfunction through NOx/NF-?B/CAM signaling, increased mononuclear-endothelial adhesion, and exacerbated atherosclerosis in mice with remnant kidneys. These results suggest a novel causative role for therapeutic iron in cardiovascular complications in patients with CKD. PMID:24722448

Kuo, Ko-Lin; Hung, Szu-Chun; Lee, Tzong-Shyuan; Tarng, Der-Cherng

2014-11-01

159

Understanding Marine Mussel Adhesion  

PubMed Central

In addition to identifying the proteins that have a role in underwater adhesion by marine mussels, research efforts have focused on identifying the genes responsible for the adhesive proteins, environmental factors that may influence protein production, and strategies for producing natural adhesives similar to the native mussel adhesive proteins. The production-scale availability of recombinant mussel adhesive proteins will enable researchers to formulate adhesives that are water-impervious and ecologically safe and can bind materials ranging from glass, plastics, metals, and wood to materials, such as bone or teeth, biological organisms, and other chemicals or molecules. Unfortunately, as of yet scientists have been unable to duplicate the processes that marine mussels use to create adhesive structures. This study provides a background on adhesive proteins identified in the blue mussel, Mytilus edulis, and introduces our research interests and discusses the future for continued research related to mussel adhesion. PMID:17990038

Roberto, Francisco F.

2007-01-01

160

ELAM1 is an adhesion molecule for skin-homing T cells  

Microsoft Academic Search

ENDOTHELIAL cell leukocyte adhesion molecule-1 (ELAM-1) has been described as an inducible endothelial cell-adhesion molecule for neutrophils, and is believed to have a key role in the extravasation of these cells at sites of acute inflammation1-3. Here we report that ELAM-1-transfected COS cells also bind a unique skin-associated subset of circulating memory T cells defined by the expression of the

Louis J. Picker; Takashi K. Kishimoto; C. Wayne Smith; R. Aaron Warnock; Eugene C. Butcher

1991-01-01

161

Oral green tea catechins transiently lower plasma glucose concentrations in female db/db mice.  

PubMed

Polyphenols, including green tea catechins, are secondary plant compounds often discussed in the context of health-promoting potential. Evidence for such effects is mainly derived from epidemiological and cell culture studies. The aim of the present study was to investigate antidiabetic, antiadipogenic, and anti-inflammatory effects at nonpharmacological doses in an obese diabetic mouse model that exerts early relevant clinical signs of non-insulin-dependent diabetes mellitus. Female db/db mice received a flavonoid-poor diet either without additive, with rosiglitazone (RSG, 0.02 g/kg diet), or with green tea extract (low-dose green tea extract [LGTE] and high-dose green tea extract [HGTE], 0.1 and 1 g/kg diet). Food and water were freely available. The body weight was monitored weekly. Blood was sampled (12-h fasted) from the tail vein on day 28 and analyzed for glucose, cholesterol, triacylglycerol, nonesterified fatty acids, insulin, adiponectin, and soluble intercellular adhesion molecule-1 (sICAM-1). Blood glucose was also analyzed on day 14. Furthermore, sICAM-1 release was investigated in tumor necrosis factor alpha-stimulated EAhy926 cells. After 14 days, fasting glycemia was improved by RSG or HGTE supplementation compared to controls. However, at the end of the study (day 28), only RSG exhibited glucose-lowering effects and induced plasma adiponectin concentrations, paralleled by higher body weight gain and reduced periuterine fat pads compared to controls. However, only GTE treatment reduced sICAM-1 release in vitro and in vivo. Nonpharmacological HGTE supplementation in db/db mice caused (1) no adiponectin-inducing or antiadipogenic effects, (2) reduced sICAM-1 release, thereby potentially exerting anti-inflammatory effects in the progressive diabetic state, and (3) a transient improvement in glycemia. PMID:23514230

Wein, Silvia; Schrader, Eva; Rimbach, Gerald; Wolffram, Siegfried

2013-04-01

162

Intercellular adhesion molecule 1 (ICAM-1) is synergistically activated by TNF-alpha and IFN-gamma responsive sites.  

PubMed

Human ICAM-1 expression can be upregulated by IFN-gamma or TNF-alpha and is synergistically increased by a combination of both cytokines. Transient transfections of ICAM-1/luciferase constructs identified two regulatory regions mediating the cytokine responses and both were found to be necessary for synergism. Using electrophoretic mobility shift assays and specific antibodies we observed that the NF-kappa B like sequence at -187 bound both p65/p50 and p65/c-Rel in the presence of TNF-alpha, while the interferon responsive region at -75 bound Stat1 alpha (p91). Treatment with IFN-gamma together with TNF-alpha did not lead to any additional or enhanced bands, suggesting that both transcription factor complexes function independently to increase the transcription initiation. PMID:8530159

Jahnke, A; Johnson, J P

1995-07-01

163

Interleukin-6 and intercellular cell adhesion molecule-1 expression remains elevated in revived live endothelial cells following spaceflight.  

PubMed

The effects of spaceflight on cardiovascular health are not necessarily seen immediately after astronauts have returned but can be delayed. It is important to investigate the long term effects of spaceflight on protein and gene expression of inflammation and endothelial activation as a predictor for the development of atherosclerosis and potential cardiovascular problems. The objectives of this study were to investigate the (a) protein and gene expression of inflammation and endothelial activation, (b) expression of nuclear factor kappa B (NF?B), signal transducer and activator of transcription-3 (STAT-3) and endothelial nitric oxide synthase (eNOS) in human umbilical vein endothelial cells (HUVEC) 3 months post-space flight travel compared to ground controls. HUVEC cultured on microcarriers in fluid processing apparatus were flown to the International Space Station (ISS) by the Soyuz TMA-11 rocket. After landing, the cells were detached from microcarriers and recultured in T-25 cm(2) culture flasks (Revived HUVEC). Soluble protein expression of IL-6, TNF-?, ICAM-1, VCAM-1 and e-selectin were measured by ELISA. Gene expression of these markers and in addition NF?B, STAT-3 and eNOS were measured. Spaceflight induced IL-6 and ICAM-1 remain elevated even after 3 months post spaceflight travel and this is mediated via STAT-3 pathway. The downregulation of eNOS expression in revived HUVEC cells suggests a reduced protection of the cells and the surrounding vessels against future insults that may lead to atherosclerosis. It would be crucial to explore preventive measures, in relation to atherosclerosis and its related complications. PMID:24362480

Muid, S; Froemming, G R A; Ali, A M; Nawawi, H

2013-12-01

164

MicroRNA-572 Improves Early Post-Operative Cognitive Dysfunction by Down-Regulating Neural Cell Adhesion Molecule 1  

PubMed Central

Post-operative cognitive dysfunction (POCD) is a commonly-seen postoperative complication in elderly patients. However, the underlying mechanisms of POCD remain unclear. miRNAs, which are reported to be involved in the pathogenesis of the nervous system diseases, may also affect POCD. In this study, miRNA microarray technology was used to analyze the circulating miRNA expression profile of POCD patients. Among the altered miRNAs, miR-572 had the greatest decrease, which was also verified in vivo in rat POCD model. Further analysis found that miR-572 could regulate the expression of NCAM1 in the hippocampal neurons and interfering miR-572 expression could facilitate the restoration of cognitive function in vivo. Moreover, clinical correlation analysis found that the miR-572 expression was associated with the incidence of POCD. Collectively, miR-572 is involved in the development and restoration of POCD and it may serve as a biological marker for early diagnosis of POCD. PMID:25680004

Yu, Xiya; Liu, Shupeng; Li, Jinbao; Fan, Xiaohua; Chen, Yuanjie; Bi, Xiaoying; Liu, Shanrong; Deng, Xiaoming

2015-01-01

165

Prevention of Leukostasis and Vascular Leakage in Streptozotocin-Induced Diabetic Retinopathy via Intercellular Adhesion Molecule1 Inhibition  

Microsoft Academic Search

Diabetic retinopathy is a leading cause of adult vision loss and blindness. Much of the retinal damage that characterizes the disease results from retinal vascular leakage and nonperfusion. This study shows that diabetic retinal vascular leakage and nonperfusion are temporally and spatially associated with retinal leukocyte stasis (leukostasis) in the rat model of streptozotocin-induced diabetes. Retinal leukostasis increases within days

Kazuaki Miyamoto; Samer Khosrof; Sven-Erik Bursell; Richard Rohan; Toshinori Murata; Allen C. Clermont; Lloyd Paul Aiello; Yuichiro Ogura; Anthony P. Adamis

1999-01-01

166

Intercellular Adhesion Molecule-1 in T cell differentiation and as a target for peptide therapy of type 1 diabetes  

E-print Network

CD4+ T cells are essential for proper function of the immune system. Many facets of an immune response are dependent on help from CD4+ T cells to become activated and exhibit effector function. Memory CD4+ T cells are a differentiated subset...

Dotson, Abby Louise

2012-05-31

167

Internal Tissue Adhesive Approved  

MedlinePLUS

... on this page, please enable JavaScript. Internal Tissue Adhesive Approved TissuGlu connects tissue flaps stemming from surgery ... and Drug Administration has approved the first tissue adhesive for internal use. Known as TissuGlu, surgeons can ...

168

PH dependent adhesive peptides  

DOEpatents

A novel peptide adhesive motif is described that requires no receptor or cross-links to achieve maximal adhesive strength. Several peptides with different degrees of adhesive strength have been designed and synthesized using solid phase chemistries. All peptides contain a common hydrophobic core sequence flanked by positively or negatively charged amino acids sequences.

Tomich, John; Iwamoto, Takeo; Shen, Xinchun; Sun, Xiuzhi Susan

2010-06-29

169

Pentoxifylline Decreases Serum Level of Adhesion Molecules in Atherosclerosis Patients  

PubMed Central

Background: Inflammation is involved in development, progression, and complications of atherosclerotic disease. Clinical studies have indicated that the level of monocyte chemoattractant protein 1 (MCP-1), IL-18, and adhesion molecules correlates with the severity of atherosclerosis and can predict future cardiovascular events. Experimental studies have shown pentoxifylline (PTX) reduces these factors in animal models. The purpose of the present pilot study was to evaluate effect of PTX on a group of inflammatory biomarkers in patients with coronary artery disease (CAD). Methods: Forty patients with angiographically documented CAD, who fulfilled inclusion and exclusion criteria, were entered in the double-blind, randomized, pilot clinical study. The patients were randomly given PTX (400 mg three times daily) or placebo (3 tab/day) for 2 months. Serum concentrations of MCP-1, IL-18, intercellular adhesion Molecule 1 (ICAM-1), and vascular cell adhesion molecule 1 (VCAM-1) were measured before and at the end of intervention by enzyme-linked immunosorbant assay. Results: Our study showed that the serum levels of ICAM-1 and VCAM-1 was decreased in the study population after two-month treatment (P<0.05). Conclusion: Based on the results of our pilot study, administration of PTX in CAD patients significantly decreases adhesion molecules levels. PMID:24375159

Mohammadpour, Amir Hooshang; Falsoleiman, Homa; Shamsara, Jamal; Abadi, Ghazaleh Allah; Rasooli, Ramin; Ramezani, Mohammad

2014-01-01

170

Some fundamentals of adhesion in synthetic adhesives.  

PubMed

Various adhesion mechanisms that have been understood in the field of synthetic adhesives are described and these are linked with situations relevant to fouling issues. The review mainly deals with mechanical aspects of adhesion phenomena, with an emphasis on the role of the elasticity of the bodies, called substrata, attached by adhesive. The consequences of thin film geometry of the adhesive material are described, such as various heterogeneous deformations upon traction. The importance of the bonding process is discussed, as well as some examples of non-wetting surfaces. Some basic ideas of fracture mechanics are provided and in particular, the behavior of layered systems is discussed. Rolling sticky objects and peeled (flexible) adhesive tapes display similar mechanisms and it is shown how they differ from the normal separation of rigid bodies. Some issues directly related to fouling issues are also discussed, such as forces and torques acting on shells, the advantages of gregarious settlement behavior and concepts for fouling release and antifouling. PMID:14618704

Gay, Cyprien

2003-04-01

171

Circulating Blood Markers and Functional Impairment in Peripheral Arterial Disease  

PubMed Central

OBJECTIVES To determine whether higher levels of inflammatory blood markers, D-dimer, and homocysteine were associated with greater impairment in lower extremity functioning in persons with peripheral arterial disease (PAD). DESIGN Cross-sectional. SETTING Three Chicago-area medical centers. PARTICIPANTS Four hundred twenty-three persons with PAD (ankle-brachial index (ABI) <0.90). MEASUREMENTS Lower extremity performance was assessed using the 6-minute walk and with usual- and fast-paced 4-m walking speed. Blood markers were D-dimer, C-reactive protein (CRP), interleukin-6 (IL-6), soluble vascular cellular adhesion molecule-1 (sVCAM-1), soluble intracellular adhesion molecule-1 (sICAM-1), and homocysteine. Calf muscle area was measured using computed tomography. RESULTS Adjusting for confounders, higher levels of D-dimer (P<.001), IL-6 (P<.001), sVCAM-1 (P=.006), CRP (P=.01), homocysteine (P=.004), and sICAM-1 (P=.046) were associated with poorer 6-minute walk performance. Higher levels of D-dimer (P<.001), IL-6 (P=.003), sVCAM-1 (P=.001), and homocysteine (P=.005) were associated with slower usual-paced 4-m walking speed. Higher levels of D-dimer, sVCAM-1, sICAM-1, IL-6, and homocysteine were associated with slower fast-paced walking speed. Results were attenuated after additional adjustment for calf muscle area. CONCLUSION Higher levels of inflammation and D-dimer were associated with poorer lower extremity performance in participants with PAD, independent of confounders including ABI. PMID:18662216

McDermott, Mary M.; Liu, Kiang; Ferrucci, Luigi; Tian, Lu; Guralnik, Jack M.; Green, David; Tan, Jin; Liao, Yihua; Pearce, William H.; Schneider, Joseph R.; McCue, Kimberly; Ridker, Paul; Rifai, Nader; Criqui, Michael H.

2009-01-01

172

White blood cell deformation and firm adhesion  

NASA Astrophysics Data System (ADS)

For a white blood cell (WBC) to arrive at infection sites, it forms chemical attachments with activated endothelial cells. First, it bonds with P-selectin, which holds it to the wall, but weakly; this allows the WBC to roll under the shear flow of the blood around it. Later, the WBCs bond with the stronger intracellular adhesion molecule-1 (ICAM-1); it is these ICAM bonds that allow the WBCs to fully resist the flow and stop rolling, allowing them to crawl through the endothelial wall. We model this numerically. Our model uses the immersed boundary method to represent the interaction of the shear flow with the deformable cell membrane. Receptors are on the tips of microvilli-little fingers sticking off of the cell membrane. The microvilli also deform. The receptors stochastically form and break bonds with molecules on the wall. Using this method, the history of each microvillus and its bonds can be found, as well as the distribution of the adhesion traction forces and how all of these vary with the deformability of the white blood cell. At higher shear rates, the white blood cell membrane deforms more, increasing its contact area with the surface; this effect is larger for softer membranes. We investigate how the deformability of the WBC affects the ease with which it forms firm adhesion.

Szatmary, Alex; Eggleton, Charles

2011-11-01

173

Nuclear factor kappaB-mediated down-regulation of adhesion molecules: possible mechanism for inhibitory activity of bigelovin against inflammatory monocytes adhesion to endothelial cells.  

PubMed

The flowers of Inula britannica L. var. chinensis (Rupr.) Reg. (Compositae) are used in traditional medicine to treat asthma, chronic bronchitis, and acute pleurisy in China and Korea. However, the pharmacological actions of Inula britannica L. var. chinensis on endothelial cells and inflammatory monocytes are not clear. In this study, we investigated whether bigelovin, a sesquiterpene lactone isolated from the flowers of Inula britannica L. var. chinensis, inhibits monocyte adhesion and adhesion molecule expression in brain endothelial cells. We measured tumor necrosis factor-alpha (TNF-alpha)-enhanced Raw264.7 monocyte binding to brain endothelial cells and the levels of cell adhesion molecules, including vascular adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and endothelial-selectin (E-selectin) on the surface of brain endothelial cells. Bigelovin significantly inhibited these in a dose-dependent manner without affecting cell viability. Furthermore, bigelovin suppressed the nuclear factor kappaB (NF-kappaB) promoter-driven luciferase activity, NF-kappaB activation, and degradation of NF-kappaB inhibitor protein alpha (IkappaBalpha). These results indicate that bigelovin inhibits inflammatory monocyte adhesion to endothelial cells and the expression of VCAM-1, ICAM-1, and E-selectin by blocking IkappaBalpha degradation and NF-kappaB activation. PMID:19429369

Nam, Kung-Woo; Oh, Goo Taeg; Seo, Eun-Kyoung; Kim, Kyeong Ho; Koo, Uk; Lee, Sung-Jin; Mar, Woongchon

2009-06-22

174

Physical Activity During Daily Life and Circulating Biomarker Levels in Patients with Peripheral Arterial Disease  

PubMed Central

Higher levels of inflammation are associated with adverse outcomes in persons with lower extremity peripheral arterial disease (PAD). This study evaluated associations of physical activity during daily life with levels of inflammatory biomarkers, D-dimer, and homocysteine in persons with PAD. Participants were 244 men and women (mean age 74.4 years ± 8.2) with PAD (ankle brachial index (ABI) < .90). C reactive protein (CRP), Interleukin-6 (IL-6), soluble Intracellular Adhesion Molecule-1 (sICAM-1), soluble Vascular Cellular Adhesion Molecule-1 (sVCAM-1), D-dimer, and homocysteine were assessed at study entry. Physical activity was objectively assessed via a vertical accelerometer, which participants wore continuously for 7 days. After adjusting for age, sex, race, body mass index, smoking, comorbidities, ABI, and other potential confounders, higher physical activity levels were associated linearly and significantly with lower levels of all measured circulating biomarkers: sVCAM-1 (p trend = 0.001); D-Dimer (p trend = 0.005); homocysteine (p trend = 0.006); IL-6 (p trend = 0.010); CRP, (p trend = 0.028); sICAM-1 (p trend = 0.033). In conclusion, higher levels of physical activity were associated independently with lower levels of inflammatory markers, homocysteine, and D-dimer in PAD patients. PMID:18940304

Craft, Lynette L.; Guralnik, Jack M.; Ferrucci, Luigi; Liu, Kiang; Tian, Lu; Criqui, Michael H.; Tan, Jin; McDermott, Mary M.

2012-01-01

175

Biomarkers of vascular function in premenopausal and recent postmenopausal women of similar age: effect of exercise training.  

PubMed

Menopause is associated with an accelerated decline in vascular function; however, whether this is an effect of age and/or menopause and how exercise training may affect this decline remains unclear. We examined a range of molecular measures related to vascular function in matched premenopausal and postmenopausal women before and after 12 wk of exercise training. Thirteen premenopausal and 10 recently postmenopausal [1.6 ± 0.3 (means ± SE) years after final menstrual period] women only separated by 3 yr (48 ± 1 vs. 51 ± 1 yr) were included. Before training, diastolic blood pressure, soluble intercellular adhesion molecule-1 (sICAM-1), and skeletal muscle expression of thromboxane A synthase were higher in the postmenopausal women compared with the premenopausal women, all indicative of impaired vascular function. In both groups, exercise training lowered diastolic blood pressure, the levels of sICAM-1, soluble vascular adhesion molecule-1 (sVCAM-1), as well as plasma and skeletal muscle endothelin-1. The vasodilator prostacyclin tended (P = 0.061) to be higher in plasma with training in the postmenopausal women only. These findings demonstrate that already within the first years after menopause, several biomarkers of vascular function are adversely altered, indicating that these biomarker changes are more related to hormonal changes than aging. Exercise training appears to have a positive impact on vascular function, as indicated by a marked improvement in the biomarker profile, in both premenopausal and postmenopausal women. PMID:24477543

Nyberg, Michael; Seidelin, Kaare; Andersen, Thomas Rostgaard; Overby, Nickie Neumann; Hellsten, Ylva; Bangsbo, Jens

2014-04-01

176

Silicone pressure-sensitive adhesives with selective adhesion characteristics  

Microsoft Academic Search

The peel, probe tack, and loop tack adhesion characteristics of peroxide-cured silicone pressure-sensitive adhesives (PSAs) are investigated with respect to adhesive composition, peroxide concentration, and type of substrate. These adhesion properties decrease with increasing benzoyl peroxide concentration and their adhesion values vary noticeably with the substrate type. However, the loop adhesion to 'difficult-to-wet' surfaces (e.g. silicone-coated substrates) can be selectively

Shaow B. Lin

1996-01-01

177

Magnetic Resonance Imaging of Endothelial Adhesion Molecules in Mouse Atherosclerosis Using Dual-Targeted Microparticles of Iron Oxide  

Microsoft Academic Search

Objective—Microparticles of iron oxide (MPIO) distort magnetic field creating marked contrast effects far exceeding their physical size. We hypothesized that antibody-conjugated MPIO would enable magnetic resonance imaging (MRI) of endothelial cell adhesion molecules in mouse atherosclerosis. Methods and Results—MPIO (4.5 m) were conjugated to monoclonal antibodies against vascular cell adhesion molecule-1 (VCAM-MPIO) or P-selectin (P-selectin-MPIO). In vitro, VCAM-MPIO bound, in

Martina A. McAteer; Jurgen E. Schneider; Ziad A. Ali; Nicholas Warrick; Christina A. Bursill; David R. Greaves; Stefan Neubauer; Keith M. Channon; Robin P. Choudhury

2010-01-01

178

Traditional herbal remedies that influence cell adhesion molecule activity.  

PubMed

Many traditional medicines have demonstrated immune activity, however, research has largely neglected their effects on cell adhesion molecules (CAMs). This review reports on extracts from 37 medicinal plant species, similar to or replicating traditional preparations, that up- or downregulate either gene or protein activity of CAMs. The majority of the investigations were in vitro, primarily of the immunoglobulin superfamily of CAMs, specifically intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and secondarily on the integrin (CD11b or MAC-1) and selectin (E-selectin and P-selectin) families of CAMs. The following plant species have demonstrated modulation of multiple CAMs: Artemisia asiatica, Boswellia serrata, Canscora decussata, Cinnamomum povectum, Dehaasia incrassate, Ganoderma lucidum, Ginkgo biloba, Hypericum perforatum, Juglans regia, Lycopus lucidus, Panax notoginseng, Rheum undulatum, Salvia miltiorrhiza. Many other species have documented activity on one CAM. Currently there are limited in vivo/ex vivo investigations, including a clinical trial on Mahonia aquifolium. Although further evidence is needed, the data suggest that the reviewed botanical medicines may have the potential to provide therapeutic potential in disease processes involving CAMs. Additionally, the reported success of many of these plant extracts by traditional cultures and modern phytotherapists may involve the modulation of CAMs. PMID:21105177

Spelman, K; Aldag, R; Hamman, A; Kwasnik, E M; Mahendra, M A; Obasi, T M; Morse, J; Williams, E J

2011-04-01

179

Dynamic Adhesion of T Lymphocytes to Endothelial Cells Revealed by Atomic Force Microscopy  

PubMed Central

The recruitment of T lymphocytes to lymphoid organs or sites of inflammation is a crucial step in adaptive immunity. These processes require endothelial activation and expression of adhesion molecules, including E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, the complete characterization of the adhesion strength and dynamics between lymphocytes and endothelial cells has been hampered by the lack of sensitive quantitative techniques. Here we report on the application of atomic force microscopy to characterize the interaction between individual pairs of living T lymphocytes (i.e., Jurkat cells) and human umbilical vein endothelial cells (HUVECs). The detachment of individual cell–cell conjugates was a complex process involving several step-like rupture events and the viscoelastic deformation of cells on the scale of several microns. Adhesion between Jurkat cells and activated endothelial cells increased with compression force and contact time, with the most dramatic changes occurring within the first half second of contact. After 0.25 sec of contact, E-selectin, ICAM-1, and VCAM-1 contributed to 18%, 39%, and 41% of total adhesion strength, respectively, suggesting that ICAM-1 and VCAM-1 contributed more than the selectins in supporting cell attachment. PMID:16946399

Zhang, Xiaohui; Wojcikiewicz, Ewa P.; Moy, Vincent T.

2008-01-01

180

Phospholipase D1 mediates lymphocyte adhesion and migration in experimental autoimmune encephalomyelitis.  

PubMed

Lymphocyte adhesion and subsequent trafficking across endothelial barriers are essential steps in various immune-mediated disorders of the CNS, including MS. The molecular mechanisms underlying these processes, however, are still unknown. Phospholipase D1 (PLD1), an enzyme that generates phosphatidic acid through hydrolysis of phosphatidylcholine and additionally yields choline as a product, has been described as regulator of the cell mobility. By using PLD1-deficient mice, we investigated the functional significance of PLD1 for lymphocyte adhesion and migration in vitro and after myelin oligodendrocyte glycoprotein (MOG)35-55 -induced EAE, a model of human MS. The lack of PLD1 reduced chemokine-mediated static adhesion of lymphocytes to the endothelial adhesion molecules vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) in vitro, and was accompanied by a decreased migratory capacity in both blood brain barrier and cell migration models. Importantly, PLD1 is also relevant for the recruitment of immune cells into the CNS in vivo since disease severity after EAE was significantly attenuated in PLD1-deficient mice. Furthermore, PLD1 expression could be detected on lymphocytes in MS patients. Our findings suggest a critical function of PLD1-dependent intracellular signaling cascades in regulating lymphocyte trafficking during autoimmune CNS inflammation. PMID:24811005

Göbel, Kerstin; Schuhmann, Michael K; Pankratz, Susann; Stegner, David; Herrmann, Alexander M; Braun, Attila; Breuer, Johanna; Bittner, Stefan; Ruck, Tobias; Wiendl, Heinz; Kleinschnitz, Christoph; Nieswandt, Bernhard; Meuth, Sven G

2014-08-01

181

21 CFR 878.4010 - Tissue adhesive.  

Code of Federal Regulations, 2010 CFR

...Identification . A tissue adhesive for the topical...skin edges. Tissue adhesives for the topical... (b) Tissue adhesive for non-topical...Identification . A tissue adhesive for non-topical use, including adhesives intended...

2010-04-01

182

21 CFR 878.4010 - Tissue adhesive.  

Code of Federal Regulations, 2012 CFR

...Identification . A tissue adhesive for the topical...skin edges. Tissue adhesives for the topical... (b) Tissue adhesive for non-topical...Identification . A tissue adhesive for non-topical use, including adhesives intended...

2012-04-01

183

21 CFR 878.4010 - Tissue adhesive.  

Code of Federal Regulations, 2011 CFR

...Identification . A tissue adhesive for the topical...skin edges. Tissue adhesives for the topical... (b) Tissue adhesive for non-topical...Identification . A tissue adhesive for non-topical use, including adhesives intended...

2011-04-01

184

21 CFR 878.4010 - Tissue adhesive.  

Code of Federal Regulations, 2014 CFR

...Identification. A tissue adhesive for the topical...skin edges. Tissue adhesives for the topical... (b) Tissue adhesive for non-topical...Identification. A tissue adhesive for non-topical use, including adhesives intended...

2014-04-01

185

21 CFR 878.4010 - Tissue adhesive.  

Code of Federal Regulations, 2013 CFR

...Identification . A tissue adhesive for the topical...skin edges. Tissue adhesives for the topical... (b) Tissue adhesive for non-topical...Identification . A tissue adhesive for non-topical use, including adhesives intended...

2013-04-01

186

Citreoviridin enhances tumor necrosis factor-?-induced adhesion of human umbilical vein endothelial cells.  

PubMed

Endothelial adhesion plays an important role in the process of atherosclerosis, which is regulated by endothelial adhesion molecules and chemoattractant molecules. In some areas of China, citreoviridin (CIT) is considered a risk factor for the development of atherosclerosis. Here, we investigated the role of CIT in adhesion of human umbilical vein endothelial cells (HUVECs) together with the stimulation of tumor necrosis factor-? (TNF-?). Adhesion of HUVECs to monocytes was analyzed by coculture experiments using U937 cells labeled with 2,7-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethylester. The expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin was determined by Western blot and enzyme-linked immunosorbent assay (ELISA). The expression of monocyte chemoattractant protein-1 (MCP-1) was measured by reverse transcription polymerase chain reaction and ELISA. The activation of nuclear factor-?B (NF-?B) was assessed by Western blot and immunofluorescence staining. CIT markedly increased TNF-?-induced HUVECs adhesion to monocytes and the expression levels of ICAM-1, VCAM-1, E-selectin, and MCP-1. TNF-?-induced nuclear translocation of NF-?B in HUVECs was significantly elevated by CIT. Our study demonstrates that CIT upregulates TNF-?-induced endothelial adhesion via increasing activation of NF-?B, which results in the expression of ICAM-1, VCAM-1, E-selectin, and MCP-1. CIT plays a pivotal role in the process of endothelial cell adhesion and may thereby play an important role in the improvement of atherosclerosis in areas of China that have a high prevalence of CIT contamination and atherosclerosis. PMID:23524880

Hou, Haifeng; Zhou, Ru; Jia, Qiang; Li, Qunwei; Kang, Li; Jiao, Peng; Li, Dong; Jiang, Baofa

2015-03-01

187

Role of inflammation in previously untreated macular edema with branch retinal vein occlusion  

PubMed Central

Background The association of inflammatory factors and the aqueous flare value with macular edema in branch retinal vein occlusion (BRVO) patients remains unclear. The relationship between the aqueous flare value and the vitreous fluid levels of vascular endothelial growth factor (VEGF), interleukin (IL)-6, monocyte chemotactic protein (MCP)-1, soluble intercellular adhesion molecule 1 (sICAM-1), and soluble VEGF receptor-2 (sVEGFR-2) was evaluated to investigate the role of inflammation in BRVO associated with macular edema. Aqueous flare values and the vitreous levels of VEGF, IL-6, MCP-1, sICAM-1, and sVEGFR-2 were compared between previously untreated patients with BRVO and patients with macular hole (MH). Methods Vitreous samples were obtained from 45 patients during vitreoretinal surgery (28 patients with BRVO and 17 with MH), and the levels of VEGF, IL-6, MCP-1, sICAM-1, and sVEGFR-2 were measured by enzyme-linked immunosorbent assay. Retinal ischemia was evaluated by measuring the area of capillary non-perfusion using fluorescein angiography and the Scion Image program. Aqueous flare values were measured with a laser flare meter and macular edema was examined by optical coherence tomography. Results The median aqueous flare value was significantly higher in the BRVO group (12.1 photon counts/ms) than in the MH group (4.5 photon counts/ms, P?

2014-01-01

188

Adhesive Contact Sweeper  

NASA Technical Reports Server (NTRS)

Adhesive contact sweeper removes hair and particles vacuum cleaner leaves behind, without stirring up dust. Also cleans loose rugs. Sweeper holds commercially available spools of inverted adhesive tape. Suitable for use in environments in which air kept free of dust; optics laboratories, computer rooms, and areas inhabited by people allergic to dust. For carpets, best used in tandem with vacuum cleaner; first pass with vacuum cleaner removes coarse particles, and second pass with sweeper extracts fine particles. This practice extends useful life of adhesive spools.

Patterson, Jonathan D.

1993-01-01

189

Focal adhesions in osteoneogenesis  

PubMed Central

As materials technology and the field of tissue engineering advances, the role of cellular adhesive mechanisms, in particular the interactions with implantable devices, becomes more relevant in both research and clinical practice. A key tenet of medical device technology is to use the exquisite ability of biological systems to respond to the material surface or chemical stimuli in order to help develop next-generation biomaterials. The focus of this review is on recent studies and developments concerning focal adhesion formation in osteoneogenesis, with an emphasis on the influence of synthetic constructs on integrin mediated cellular adhesion and function. PMID:21287830

Biggs, M.J.P; Dalby, M.J

2010-01-01

190

Cell adhesion force microscopy  

PubMed Central

The adhesion forces of cervical carcinoma cells in tissue culture were measured by using the manipulation force microscope, a novel atomic force microscope. The forces were studied as a function of time and temperature for cells cultured on hydrophilic and hydrophobic polystyrene substrates with preadsorbed proteins. The cells attached faster and stronger at 37°C than at 23°C and better on hydrophilic than on hydrophobic substrates, even though proteins adsorb much better to the hydrophobic substrates. Because cell adhesion serves to control several stages in the cell cycle, we anticipate that the manipulation force microscope can help clarify some cell-adhesion related issues. PMID:9892657

Sagvolden, G.; Giaever, I.; Pettersen, E. O.; Feder, J.

1999-01-01

191

Enzyme modified soy flour adhesives  

Microsoft Academic Search

Soy protein based adhesives have not been used extensively in wood products since the 1960's because of inferior performance, stability, and water resistance issues relative to petroleum based adhesives. The early soy protein adhesives were made from defatted flours and were dispersed in alkaline solutions to denature proteins and make more polar groups available for adhesion. Recent research has focused

John F. Schmitz

2009-01-01

192

Adhesion of Lunar Dust  

NASA Astrophysics Data System (ADS)

This paper reviews the physical characteristics of lunar dust and the effects of various fundamental forces acting on dust particles on surfaces in a lunar environment. There are transport forces and adhesion forces after contact. Mechanical forces (i.e., from rover wheels, astronaut boots and rocket engine blast) and static electric effects (from UV photo-ionization and/or tribo-electric charging) are likely to be the major contributors to the transport of dust particles. If fine regolith particles are deposited on a surface, then surface energy-related (e.g., van der Walls) adhesion forces and static-electric-image forces are likely to be the strongest contributors to adhesion. Some measurement techniques are offered to quantify the strength of adhesion forces. And finally some dust removal techniques are discussed.

Walton, Otis R.

2007-04-01

193

Adhesion of Lunar Dust  

NASA Technical Reports Server (NTRS)

This paper reviews the physical characteristics of lunar dust and the effects of various fundamental forces acting on dust particles on surfaces in a lunar environment. There are transport forces and adhesion forces after contact. Mechanical forces (i.e., from rover wheels, astronaut boots and rocket engine blast) and static electric effects (from UV photo-ionization and/or tribo-electric charging) are likely to be the major contributors to the transport of dust particles. If fine regolith particles are deposited on a surface, then surface energy-related (e.g., van der Walls) adhesion forces and static-electric-image forces are likely to be the strongest contributors to adhesion. Some measurement techniques are offered to quantify the strength of adhesion forces. And finally some dust removal techniques are discussed.

Walton, Otis R.

2007-01-01

194

Optical adhesive property study  

SciTech Connect

Tests were performed to characterize the mechanical and thermal properties of selected optical adhesives to identify the most likely candidate which could survive the operating environment of the Direct Optical Initiation (DOI) program. The DOI system consists of a high power laser and an optical module used to split the beam into a number of channels to initiate the system. The DOI requirements are for a high shock environment which current military optical systems do not operate. Five candidate adhesives were selected and evaluated using standardized test methods to determine the adhesives` physical properties. EC2216, manufactured by 3M, was selected as the baseline candidate adhesive based on the test results of the physical properties.

Sundvold, P.D.

1996-01-01

195

Adhesion by Pathogenic Corynebacteria  

Microsoft Academic Search

\\u000a Pathogenic members of the genus Corynebacterium cause a wide range of serious infections in humans including diphtheria. Adhesion to host cells is a crucial step during\\u000a infection. In Corynebacterium diphtheriae, adhesion is mediated primarily by filamentous structures called pili or fimbriae that are covalently attached to the bacterial\\u000a cell wall. C. diphtheriae produces three distinct pilus structures, SpaA-, SpaD- and

Elizabeth A. Rogers; Asis Das; Hung Ton-That

196

Micromechanics of Particle Adhesion  

Microsoft Academic Search

The rapid increasing production of cohesive to very cohesive ultra- fine powders (d < 10 µm), e.g. very adhering pigment particles, micro-carriers in medicine, auxiliary materials in catalysis make technical\\u000a problems much serious like undesired adhesion in particle processing, powder handling, and desired, in agglomeration or coating.\\u000a Thus, it is very essential to understand the fundamentals of particle adhesion with

Jürgen Tomas

197

Cytotoxicity of denture adhesives  

Microsoft Academic Search

Ten commercially available denture adhesives, nine soluble formulations (six creams, three powders) and one insoluble product\\u000a (pad), were analyzed regarding the cytotoxicity profile in direct and indirect assays using L929 fibroblast cells. In the\\u000a direct assay, fibroblasts were seeded over the surface of a thick adhesive gel (5%, creams; 2.5%, powders and pad). In the\\u000a indirect assay, cells were cultured

Pedro Sousa de Gomes; Maria Helena Figueiral; Maria Helena R. Fernandes; Crispian Scully

198

Adhesive Gravitational Clustering  

E-print Network

The notion of `adhesion' has been advanced for the phenomenon of stabilization of large-scale structure emerging from gravitational instability of a cold medium. Recently, the physical origin of adhesion has been identified: a systematic derivation of the equations of motion for the density and the velocity fields leads naturally to the key equation of the `adhesion approximation' - however, under a set of strongly simplifying assumptions. In this work, we provide an evaluation of the current status of adhesive gravitational clustering and a clear explanation of the assumptions involved. Furthermore, we propose systematic generalizations with the aim to relax some of the simplifying assumptions. We start from the general Newtonian evolution equations for self-gravitating particles on an expanding Friedmann background and recover the popular `dust model' (pressureless fluid), which breaks down after the formation of density singularities; then we investigate, in a unified framework, two other models which, under the restrictions referred to above, lead to the `adhesion approximation'. We apply the Eulerian and Lagrangian perturbative expansions to these new models and, finally, we discuss some non-perturbative results that may serve as starting points for workable approximations of non-linear structure formation in the multi-stream regime. In particular, we propose a new approximation that includes, in limiting cases, the standard `adhesion model' and the Eulerian as well as Lagrangian first-order approximations.

Thomas Buchert; Alvaro Dominguez

2005-06-21

199

Leukocyte adhesion molecule and chemokine production through lipoteichoic acid recognition by toll-like receptor 2 in cultured human lymphatic endothelium  

Microsoft Academic Search

We have recently reported that the human lymphatic endothelium has toll-like receptor 4 (TLR4)-mediated lipopolysaccharide\\u000a recognition mechanisms that induce the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion\\u000a molecule-1 (VCAM-1). Although ligand engagement with TLR2 enables activation of the MyD88-dependent pathway similarly to TLR4,\\u000a whether TLR2 ligands such as lipoteichoic acid (LTA) trigger the activation of lymphatic endothelium

Yoshihiko Sawa; Eichi Tsuruga; Kana Iwasawa; Hiroyuki Ishikawa; Shigemitsu Yoshida

2008-01-01

200

Dry adhesives with sensing features  

NASA Astrophysics Data System (ADS)

Geckos are capable of detecting detachment of their feet. Inspired by this basic observation, a novel functional dry adhesive is proposed, which can be used to measure the instantaneous forces and torques acting on an adhesive pad. Such a novel sensing dry adhesive could potentially be used by climbing robots to quickly realize and respond appropriately to catastrophic detachment conditions. The proposed torque and force sensing dry adhesive was fabricated by mixing Carbon Black (CB) and Polydimethylsiloxane (PDMS) to form a functionalized adhesive with mushroom caps. The addition of CB to PDMS resulted in conductive PDMS which, when under compression, tension or torque, resulted in a change in the resistance across the adhesive patch terminals. The proposed design of the functionalized dry adhesive enables distinguishing an applied torque from a compressive force in a single adhesive pad. A model based on beam theory was used to predict the change in resistance across the terminals as either a torque or compressive force was applied to the adhesive patch. Under a compressive force, the sensing dry adhesive was capable of measuring compression stresses from 0.11 Pa to 20.9 kPa. The torque measured by the adhesive patch ranged from 2.6 to 10 mN m, at which point the dry adhesives became detached. The adhesive strength was 1.75 kPa under an applied preload of 1.65 kPa for an adhesive patch with an adhesive contact area of 7.07 cm2.

Krahn, J.; Menon, C.

2013-08-01

201

A role for cell adhesion in beryllium-mediated lung disease.  

PubMed

Chronic beryllium disease (CBD) is a debilitating lung disorder in which exposure to the lightweight metal beryllium (Be) causes the accumulation of beryllium-specific CD4+ T cells in the lung and formation of noncaseating pulmonary granulomas. Treatment for CBD patients who exhibit progressive pulmonary decline is limited to systemic corticosteroids, which suppress the severe host inflammatory response. Studies in the past several years have begun to highlight cell-cell adhesion interactions in the development of Be hypersensitivity and CBD. In particular, the high binding affinity between intercellular adhesion molecule 1 (I-CAM1) on lung epithelial cells and the beta(2) integrin LFA-1 on migrating lymphocytes and macrophages regulates the concerted rolling of immune cells to sites of inflammation in the lung. In this review, we discuss the evidence that implicates cell adhesion processes in onset of Be disease and the potential of cell adhesion as an intervention point for development of novel therapies. PMID:19894172

Hong-Geller, Elizabeth

2009-12-01

202

Combinatorial Investigations of Polymer Adhesion  

NSDL National Science Digital Library

In this work, we introduce a combinatorial technique that can be used to investigate adhesive interactions between a polymer and either another polymer, a ceramic, or a metal. The primary goal in the development of this technique is to design a high-throughput, parallel processing adhesion test that allows the adhesive strength dependence on multivariable environments to be determined. This combinatorial polymer adhesion test will provide qualitative and quantitative data used to determine absolute measures of adhesion as a function of the multidimensional parameter space. These results will aid industrial screening for optimal adhesives, as well as provide a unique tool for gaining a fundamental understanding of polymer adhesion. We investigate the temperature and thickness dependence of the self-adhesion of polystyrene (PS) and the adhesion between PS and poly(dimethylsiloxane) (PDMS) for demonstration of concept.

Crosby, Alfred

2001-01-01

203

Selenium inhibits 15-hydroperoxyoctadecadienoic acid-induced intracellular adhesion molecule expression in aortic endothelial cells  

Microsoft Academic Search

Increased intracellular adhesion molecule 1 (ICAM-1) expression and enhanced monocyte recruitment to the endothelium are critical steps in the early development of atherosclerosis. The 15-lipoxygenase 1 (15-LOX1) pathway can generate several proinflammatory eicosanoids that are known to enhance ICAM-1 expression within the vascular endothelium. Oxidative stress can exacerbate endothelial cell inflammatory responses by modifying arachidonic acid metabolism through the 15-LOX1

Lorraine M. Sordillo; Katie L. Streicher; Isis K. Mullarky; Jeffery C. Gandy; Wendy Trigona; Chris M. Corl

2008-01-01

204

Homocysteine, circulating vascular cell adhesion molecule and carotid atherosclerosis in postmenopausal vegetarian women and omnivores  

Microsoft Academic Search

Since the adoption of vegetarian diets as a healthy lifestyle has become popular, the cardiovascular effects of long-term vegetarianism need to be explored. The present study aimed to compare the presence and severity of carotid atherosclerosis (CA), and the blood levels of Vitamin B12, homocysteine (Hcy) and soluble vascular cell adhesion molecule-1 (sVCAM-1) between 57 healthy postmenopausal vegetarians and 61

Ta-Chen Su; Jiann-Shing Jeng; Jung-Der Wang; Pao-Ling Torng; Sue-Joan Chang; Chen-Fang Chen; Chiau-Suong Liau

2006-01-01

205

Reversible adhesion switching of porous fibrillar adhesive pads by humidity.  

PubMed

We report reversible adhesion switching on porous fibrillar polystyrene-block-poly(2-vinyl pyridine) (PS-b-P2VP) adhesive pads by humidity changes. Adhesion at a relative humidity of 90% was more than nine times higher than at a relative humidity of 2%. On nonporous fibrillar adhesive pads of the same material, adhesion increased only by a factor of ~3.3. The switching performance remained unchanged in at least 10 successive high/low humidity cycles. Main origin of enhanced adhesion at high humidity is the humidity-induced decrease in the elastic modulus of the polar component P2VP rather than capillary force. The presence of spongelike continuous internal pore systems with walls consisting of P2VP significantly leveraged this effect. Fibrillar adhesive pads on which adhesion is switchable by humidity changes may be used for preconcentration of airborne particulates, pollutants, and germs combined with triggered surface cleaning. PMID:24171547

Xue, Longjian; Kovalev, Alexander; Dening, Kirstin; Eichler-Volf, Anna; Eickmeier, Henning; Haase, Markus; Enke, Dirk; Steinhart, Martin; Gorb, Stanislav N

2013-01-01

206

Natural Underwater Adhesives  

PubMed Central

The general topic of this review is protein-based underwater adhesives produced by aquatic organisms. The focus is on mechanisms of interfacial adhesion to native surfaces and controlled underwater solidification of natural water-borne adhesives. Four genera that exemplify the broad range of function, general mechanistic features, and unique adaptations are discussed in detail: blue mussels, acorn barnacles, sandcastle worms, and freshwater caddisfly larva. Aquatic surfaces in nature are charged and in equilibrium with their environment, populated by an electrical double layer of ions as well as adsorbed natural polyelectrolytes and microbial biofilms. Surface adsorption of underwater bioadhesives likely occurs by exchange of surface bound ligands by amino acid sidechains, driven primarily by relative affinities and effective concentrations of polymeric functional groups. Most aquatic organisms exploit modified amino acid sidechains, in particular phosphorylated serines and hydroxylated tyrosines (dopa), with high-surface affinity that form coordinative surface complexes. After delivery to the surfaces as a fluid, permanent natural adhesives solidify to bear sustained loads. Mussel plaques are assembled in a manner superficially reminiscent of in vitro layer-by-layer strategies, with sequentially delivered layers associated through Fe(dopa)3 coordination bonds. The adhesives of sandcastle worms, caddisfly larva, and barnacles may be delivered in a form somewhat similar to in vitro complex coacervation. Marine adhesives are secreted, or excreted, into seawater that has a significantly higher pH and ionic strength than the internal environment. Empirical evidence suggests these environment triggers could provide minimalistic, fail-safe timing mechanisms to prevent premature solidification (insolubilization) of the glue within the secretory system, yet allow rapid solidification after secretion. Underwater bioadhesives are further strengthened by secondary covalent curing. PMID:21643511

Stewart, Russell J.; Ransom, Todd C.; Hlady, Vladimir

2011-01-01

207

Complement activation and kidney injury molecule-1-associated proximal tubule injury in severe preeclampsia.  

PubMed

Kidney injury with proteinuria is a characteristic feature of preeclampsia, yet the nature of injury in specific regions of the nephron is incompletely understood. Our study aimed to use existing urinary biomarkers to describe the pattern of kidney injury and proteinuria in pregnancies affected by severe preeclampsia. We performed a case-control study of pregnant women from Brigham and Women's Hospital from 2012 to 2013. We matched cases of severe preeclampsia (n=25) 1:1 by parity and gestational age to 2 control groups with and without chronic hypertension. Urinary levels of kidney injury molecule-1 and complement components (C3a, C5a, and C5b-9) were measured by enzyme-linked immunosorbent assay, and other markers (albumin, ?2 microglobulin, cystatin C, epithelial growth factor, neutrophil gelatinase-associated lipocalin, osteopontin, and uromodulin) were measured simultaneously with a multiplex electrochemiluminescence assay. Median values between groups were compared with the Wilcoxon signed-rank test and correlations with Spearman correlation coefficient. Analysis of urinary markers revealed higher excretion of albumin and kidney injury molecule-1 and lower excretion of neutrophil gelatinase-associated lipocalin and epithelial growth factor in severe preeclampsia compared with chronic hypertension and healthy controls. Among subjects with severe preeclampsia, urinary excretion of complement activation products correlated most closely with kidney injury molecule-1, a specific marker of proximal tubule injury (C5a: r=0.60; P=0.001; and C5b-9: r=0.75; P<0.0001). Taken together, we describe a pattern of kidney injury in severe preeclampsia that is characterized by glomerular impairment and complement-mediated inflammation and injury, possibly localized to the proximal tubule in association with kidney injury molecule-1. PMID:24958504

Burwick, Richard M; Easter, Sarah Rae; Dawood, Hassan Y; Yamamoto, Hidemi S; Fichorova, Raina N; Feinberg, Bruce B

2014-10-01

208

Elastomer toughened polyimide adhesives  

NASA Technical Reports Server (NTRS)

A rubber-toughened addition-type polyimide composition is disclosed which has excellent high temperature bonding characteristics in the fully cured state, and improved peel strength and adhesive fracture resistance physical property characteristics. The process for making the improved adhesive involves preparing the rubber containing amic acid prepolymer by chemically reacting an amine-terminated elastomer and an aromatic diamine with an aromatic dianhydride with which a reactive chain stopper anhydride was mixed, and utilizing solvent or mixture of solvents for the reaction.

St.clair, A. K.; St.clair, T. L. (inventors)

1983-01-01

209

Biomimetic Adhesive Polymers Based on Mussel Adhesive Proteins  

Microsoft Academic Search

Nature provides many outstanding examples of adhesive strategies from which chemists and material scientists can draw inspiration in their pursuit of new adhesive materials. As described in other chapters of this book, detailed studies of the adhesive mechanisms of geckos, mussels and other organisms during the past several decades have enhanced our understanding of the underlying physicochemical principles to the

BRUCE P. LEE; JEFFREY L. DALSIN; PHILLIP B. MESSERSMITH

210

3-D foam adhesive deposition  

NASA Technical Reports Server (NTRS)

Bonding method, which reduces amount and weight of adhesive, is applicable to foam-filled honeycomb constructions. Novel features of process include temperature-viscosity control and removal of excess adhesive by transfer to cellophane film.

Lemons, C. R.; Salmassy, O. K.

1976-01-01

211

Osteoblast adhesion on nanophase ceramics  

Microsoft Academic Search

Osteoblast adhesion on nanophase alumina (Al2O3) and titania (TiO2) was investigated in vitro. Osteoblast adhesion to nanophase alumina and titania in the absence of serum from Dulbecco’s modified Eagle medium (DMEM) was significantly (P<0.01) less than osteoblast adhesion to alumina and titania in the presence of serum. In the presence of 10% fetal bovine serum in DMEM osteoblast adhesion on

Thomas J Webster; Richard W Siegel; Rena Bizios

1999-01-01

212

Rapid adhesive bonding concepts  

NASA Technical Reports Server (NTRS)

Adhesive bonding in the aerospace industry typically utilizes autoclaves or presses which have considerable thermal mass. As a consequence, the rates of heatup and cooldown of the bonded parts are limited and the total time and cost of the bonding process is often relatively high. Many of the adhesives themselves do not inherently require long processing times. Bonding could be performed rapidly if the heat was concentrated in the bond lines or at least in the adherends. Rapid adhesive bonding concepts were developed to utilize induction heating techniques to provide heat directly to the bond line and/or adherends without heating the entire structure, supports, and fixtures of a bonding assembly. Bonding times for specimens are cut by a factor of 10 to 100 compared to standard press bonding. The development of rapid adhesive bonding for lap shear specimens (per ASTM D1003 and D3163), for aerospace panel bonding, and for field repair needs of metallic and advanced fiber reinforced polymeric matrix composite structures are reviewed.

Stein, B. A.; Tyeryar, J. R.; Hodges, W. T.

1984-01-01

213

Adhesion by pathogenic corynebacteria.  

PubMed

Pathogenic members of the genus Corynebacterium cause a wide range of serious infections in humans including diphtheria. Adhesion to host cells is a crucial step during infection. In Corynebacterium diphtheriae, adhesion is mediated primarily by filamentous structures called pili or fimbriae that are covalently attached to the bacterial cell wall. C. diphtheriae produces three distinct pilus structures, SpaA-, SpaD- and SpaH-type pili. Similar to other types, the prototype SpaA pilus consists of SpaA forming the pilus shaft and two minor pilins SpaB and SpaC located at the base and at the tip, respectively. The minor pilins SpaB/SpaC are critical for bacterial binding to human pharyngeal cells, and thus represent the major adhesins of corynebacteria. Like pili of many other gram-positive microbes, the assembly of corynebacterial pili occurs by a two-step mechanism, whereby pilins are covalently polymerized by a transpeptidase enzyme named pilin-specific sortase and the generated pilus polymer is subsequently anchored to the cell wall peptidoglycan via the base pilin by the housekeeping sortase or a non-polymerizing sortase. This chapter reviews the current knowledge of corynebacterial adhesion, with a specific focus on pilus structures, their assembly, and the mechanism of adhesion mediated by pili. PMID:21557059

Rogers, Elizabeth A; Das, Asis; Ton-That, Hung

2011-01-01

214

Osteoblast adhesion on biomaterials  

Microsoft Academic Search

The development of tissue engineering in the field of orthopaedic surgery is now booming. Two fields of research in particular are emerging: the association of osteo-inductive factors with implantable materials; and the association of osteogenic stem cells with these materials (hybrid materials). In both cases, an understanding of the phenomena of cell adhesion and, in particular, understanding of the proteins

K. Anselme

2000-01-01

215

Adhesion of microcapsules  

E-print Network

The adhesion of microcapsules to an attractive contact potential is studied theoretically. The axisymmetric shape equations are solved numerically. Beyond a universal threshold strength of the potential, the contact radius increases like a square root of the strength. Scaling functions for the corresponding amplitudes are derived as a function of the elastic parameters.

Peter Graf; Reimar Finken; Udo Seifert

2006-06-14

216

Adhesion molecules and receptors  

Technology Transfer Automated Retrieval System (TEKTRAN)

Adhesion molecules are necessary for leukocyte trafficking and differentiation. They serve to initiate cell-cell interactions under conditions of shear, and they sustain the cell-cell and cell-matrix interactions needed for cellular locomotion. They also can serve directly as signaling molecules act...

217

Novel adhesive tapes for semiconductor manufacturing process  

Microsoft Academic Search

AbstracG To facilitate fabrication of reliable semiconductor devices, adhesive tapes were investigated. Formulated adhesives contained acrylic polymer and epoxy resins. Through a novel design of adhesives, thew versatile adhesive tapes, lint as a pressure sensitive adhesive (PSA) and later as an adhesive material of semiconductors, have been developed. One item was studied as a die bonding tape with Wlthermd dual

Takashi Sugino; N. Saiki; Tomonori Shinoda; O. Yamazaki

2004-01-01

218

Coating Reduces Ice Adhesion  

NASA Technical Reports Server (NTRS)

The Shuttle Ice Liberation Coating (SILC) has been developed to reduce the adhesion of ice to surfaces on the space shuttle. SILC, when coated on a surface (foam, metal, epoxy primer, polymer surfaces), will reduce the adhesion of ice by as much as 90 percent as compared to the corresponding uncoated surface. This innovation is a durable coating that can withstand several cycles of ice growth and removal without loss of anti-adhesion properties. SILC is made of a binder composed of varying weight percents of siloxane(s), ethyl alcohol, ethyl sulfate, isopropyl alcohol, and of fine-particle polytetrafluoroethylene (PTFE). The combination of these components produces a coating with significantly improved weathering characteristics over the siloxane system alone. In some cases, the coating will delay ice formation and can reduce the amount of ice formed. SILC is not an ice prevention coating, but the very high water contact angle (greater than 140 ) causes water to readily run off the surface. This coating was designed for use at temperatures near -170 F (-112 C). Ice adhesion tests performed at temperatures from -170 to 20 F (-112 to -7 C) show that SILC is a very effective ice release coating. SILC can be left as applied (opaque) or buffed off until the surface appears clear. Energy dispersive spectroscopy (EDS) and x-ray photoelectron spectroscopy (XPS) data show that the coating is still present after buffing to transparency. This means SILC can be used to prevent ice adhesion even when coating windows or other objects, or items that require transmission of optical light. Car windshields are kept cleaner and SILC effectively mitigates rain and snow under driving conditions.

Smith, Trent; Prince, Michael; DwWeese, Charles; Curtis, Leslie

2008-01-01

219

Dietary patterns are associated with biochemical markers of inflammation and endothelial activation in the Multi-Ethnic Study of Atherosclerosis (MESA)2  

PubMed Central

Background Dietary patterns may influence cardiovascular disease risk through effects on inflammation and endothelial activation. Objective We examined relations between dietary patterns and markers of inflammation and endothelial activation. Design At baseline, diet (food-frequency questionnaire) and concentrations of C-reactive protein (CRP), interleukin 6 (IL-6), homocysteine, soluble intercellular adhesion molecule-1 (sICAM-1), and soluble E selectin were assessed in 5089 nondiabetic participants in the Multi-Ethnic Study of Atherosclerosis. Results Four dietary patterns were derived by using factor analysis. The fats and processed meats pattern (fats, oils, processed meats, fried potatoes, salty snacks, and desserts) was positively associated with CRP (P for trend < 0.001), IL-6 (P for trend < 0.001), and homocysteine (P for trend = 0.002). The beans, tomatoes, and refined grains pattern (beans, tomatoes, refined grains, and high-fat dairy products) was positively related to sICAM-1 (P for trend = 0.007). In contrast, the whole grains and fruit pattern (whole grains, fruit, nuts, and green leafy vegetables) was inversely associated with CRP, IL-6, homocysteine (P for trend ? 0.001), and sICAM-1 (P for trend = 0.034), and the vegetables and fish pattern (fish and dark-yellow, cruciferous, and other vegetables) was inversely related to IL-6 (P for trend = 0.009). CRP, IL-6, and homocysteine relations across the fats and processed meats and whole grains and fruit patterns were independent of demographics and lifestyle factors and were not modified by race-ethnicity. CRP and homocysteine relations were independent of waist circumference. Conclusions These results corroborate previous findings that empirically derived dietary patterns are associated with inflammation and show that these relations in an ethnically diverse population with unique dietary habits are similar to findings in more homogeneous populations. PMID:16762949

Nettleton, Jennifer A; Steffen, Lyn M; Mayer-Davis, Elizabeth J; Jenny, Nancy S; Jiang, Rui; Herrington, David M; Jacobs, David R

2010-01-01

220

The Effect of Hepatitis C Virologic Clearance on Cardiovascular Disease Biomarkers in Human Immunodeficiency Virus/Hepatitis C Virus Coinfection  

PubMed Central

Background ?Successful hepatitis C virus (HCV) treatment may reduce cardiovascular disease (CVD) risk and improve levels of CVD biomarkers produced outside the liver (nonhepatic biomarkers). Methods ?Stored serum or plasma from before and 24 weeks after end of HCV treatment (EOT) from human immunodeficiency virus (HIV)/HCV-coinfected subjects who received up to 72 weeks of peginterferon/ribavirin, 27 with and 27 without sustained virologic response (SVR) matched by race, ethnicity and sex, were tested for nonhepatic (soluble intercellular adhesion molecule-1 [sICAM-1], soluble P-selectin [sP-selectin], interleukin [IL]-6, d-dimer, and lipoprotein-associated phospholipase A2 [Lp-PLA2]) and hepatic (cholesterol and high-sensitivity C-reactive protein) CVD and macrophage activation markers (soluble CD163 [sCD163] and soluble CD14). Changes in biomarkers and their association with SVR were examined by t tests or Wilcoxon tests and regression models. Results ?Of the 54 subjects, 30 were white, 24 were black, and 44 were male. Pretreatment levels of nonhepatic biomarkers were high: sICAM-1 overall median, 439.2 ng/mL (interquartile range [IQR], 365.6–592.8]; sP-selectin, 146.7 ng/mL (IQR, 94.1–209.9), and IL-6, 2.32 pg/mL (IQR, 1.61–3.49). Thirty-seven of 52 (71%) subjects had Lp-PLA2 >235 ng/mL. Sustained virologic response was associated with decrease in sICAM-1 (P = .033) and sCD163 (P = .042); this result was attenuated after controlling for changes in the alanine aminotransferase level. At 24 weeks after EOT, 17 (63%) SVRs had Lp-PLA2 >235 ng/mL vs 25 (93%) non-SVRs (P = .021). Conclusions ?Hepatitis C virus clearance may reduce hepatic and, subsequently, systemic inflammation and CVD risk in HIV/HCV coinfection. PMID:25734172

Chew, Kara W.; Hua, Lei; Bhattacharya, Debika; Butt, Adeel A.; Bornfleth, Lorelei; Chung, Raymond T.; Andersen, Janet W.; Currier, Judith S.

2014-01-01

221

Environmentally compliant adhesive joining technology  

SciTech Connect

Adhesive joining offers one method of assembling products. Advantages of adhesive joining/assembly include distribution of applied forces, lighter weight, appealing appearance, etc. Selecting environmentally safe adhesive materials and accompanying processes is paramount in today`s business climate if a company wants to be environmentally conscious and stay in business. Four areas of adhesive joining (adhesive formulation and selection, surface preparation, adhesive bonding process, waste and pollution generation/cleanup/management) all need to be carefully evaluated before adhesive joining is selected for commercial as well as military products. Designing for six sigma quality must also be addressed in today`s global economy. This requires material suppliers and product manufacturers to work even closer together.

Tira, J.S.

1996-08-01

222

Adhesion barrier reduces postoperative adhesions after cardiac surgery.  

PubMed

Reoperation in cardiac surgery is associated with increased risk due to surgical adhesions. Application of a bioresorbable material could theoretically reduce adhesions and allow later development of a free dissection plane for cardiac reoperation. Twenty-one patients in whom a bioresorbable hyaluronic acid-carboxymethylcellulose adhesion barrier had been applied in a preceding surgery underwent reoperations, while 23 patients underwent reoperations during the same period without a prior adhesion barrier. Blinded observers graded the tenacity of the adhesions from surgical video recordings of the reoperations. No excessive bleeding requiring wound reexploration, mediastinal infection, or other complication attributable to the adhesion barrier occurred. Multiple regression analysis showed that shorter duration of the preceding surgery, non-use of cardiopulmonary bypass in the preceding surgery, and use of the adhesion barrier were significantly associated with less tenacious surgical adhesions. The use of a bioresorbable material in cardiac surgery reduced postoperative adhesions, facilitated reoperation, and did not promote complications. The use of adhesion barrier is recommended in planned staged procedures and those in which future reoperation is likely. PMID:22718712

Kaneko, Yukihiro; Hirata, Yasutaka; Achiwa, Ikuya; Morishita, Hiroyuki; Soto, Hajime; Kobayahsi, Jotaro

2012-06-01

223

Development of phosphorylated adhesives  

NASA Technical Reports Server (NTRS)

The synthesis of epoxy prepolymers containing phosphorus was carried out in such a manner as to provide adhesives containing at least 5 percent of this element. The purpose of this was to impart fire retardant properties to the adhesive. The two epoxy derivatives, bis(4-glycidyl-oxyphenyl)phenylphosphine oxide and bis(4-glycidyl-2-methoxyphenyl)phenylphosphonate, and a curing agent, bis(3-aminophenyl)methylphosphine oxide, were used in conjunction with one another and along with conventional epoxy resins and curing agents to bond Tedlar and Polyphenylethersulfone films to Kerimid-glass syntactic foam-filled honeycomb structures. Elevated temperatures are required to cure the epoxy resins with the phosphorus-contaning diamine; however, when Tedlar is being bonded, lower curing temperatures must be used to avoid shrinkage and the concomitant formation of surface defects. Thus, the phosphorus-containing aromatic amine curing agent cannot be used alone, although it is possible to use it in conjunction with an aliphatic amine which would allow lower cure temperatures to be used. The experimental epoxy resins have not provided adhesive bonds quite as strong as those provided by Epon 828 when compared in peel tests, but the differences are not very significant. It should be noted, if optimum properties are to be realized. In any case the fire retardant characteristics of the neat resin systems obtained are quite pronounced, since in most cases the self-extinguishing properties are evident almost instantly when specimens are removed from a flame.

Bilow, N.; Giants, T. W.; Jenkins, R. K.; Campbell, P. L.

1983-01-01

224

Ceramic microstructure and adhesion  

NASA Technical Reports Server (NTRS)

When a ceramic is brought into contact with a ceramic, a polymer, or a metal, strong bond forces can develop between the materials. The bonding forces will depend upon the state of the surfaces, cleanliness and the fundamental properties of the two solids, both surface and bulk. Adhesion between a ceramic and another solid are discussed from a theoretical consideration of the nature of the surfaces and experimentally by relating bond forces to interface resulting from solid state contact. Surface properties of ceramics correlated with adhesion include, orientation, reconstruction and diffusion as well as the chemistry of the surface specie. Where a ceramic is in contact with a metal their interactive chemistry and bond strength is considered. Bulk properties examined include elastic and plastic behavior in the surficial regions, cohesive binding energies, crystal structures and crystallographic orientation. Materials examined with respect to interfacial adhesive interactions include silicon carbide, nickel zinc ferrite, manganese zinc ferrite, and aluminum oxide. The surfaces of the contacting solids are studied both in the atomic or molecularly clean state and in the presence of selected surface contaminants.

Buckley, D. H.

1984-01-01

225

Elevated urinary levels of kidney injury molecule-1 among Chinese factory workers exposed to trichloroethylene  

PubMed Central

Epidemiological studies suggest that trichloroethylene (TCE) exposure may be associated with renal cancer. The biological mechanisms involved are not exactly known although nephrotoxicity is believed to play a role. Studies on TCE nephrotoxicity among humans, however, have been largely inconsistent. We studied kidney toxicity in Chinese factory workers exposed to TCE using novel sensitive nephrotoxicity markers. Eighty healthy workers exposed to TCE and 45 comparable unexposed controls were included in the present analyses. Personal TCE exposure measurements were taken over a 2-week period before urine collection. Ninety-six percent of workers were exposed to TCE below the current US Occupational Safety and Health Administration permissible exposure limit (100 ppm 8h TWA), with a mean (SD) of 22.2 (35.9) ppm. Kidney injury molecule-1 (KIM-1) and Pi-glutathione S transferase (GST) alpha were elevated among the exposed subjects as compared with the unexposed controls with a strong exposure-response association between individual estimates of TCE exposure and KIM-1 (P < 0.0001). This is the first report to use a set of sensitive nephrotoxicity markers to study the possible effects of TCE on the kidneys. The findings suggest that at relatively low occupational exposure levels a toxic effect on the kidneys can be observed. This finding supports the biological plausibility of linking TCE exposure and renal cancer. Abbreviations:GSTglutathione-S-transferaseKIM-1kidney injury molecule-1NAGN-acetyl-beta-(d)-glucosaminidaseOVMorganic vapour monitoringTCEtrichloroethyleneVEGFvascular endothelial growth factor. PMID:22665366

Vermeulen, Roel; Huang, Hanlin; Rothman, Nathaniel; Lan, Qing

2012-01-01

226

Impairment of selectin-mediated leukocyte adhesion to venular endothelium in spontaneously hypertensive rats.  

PubMed Central

The present study was designed to elucidate whether molecular mechanisms for leukocyte adhesion to microvascular endothelium may differ between spontaneously hypertensive rats and Wistar Kyoto rats. Leukocyte rolling and adhesion were investigated while monitoring venular wall shear rates in the mesenteric microcirculation stimulated with histamine or tert-butyl hydroperoxide in the two strains. In Wistar Kyoto rats, 10 microM histamine as well as 500 microM tertbutyl hydroperoxide promoted a significant reduction of venular leukocyte rolling velocity and subsequent adhesion. These changes in leukocyte behavior were blocked by monoclonal antibodies against P-selectin (PB 1.3) and against sialyl Lewis X-like carbohydrates (2H5). However, spontaneously hypertensive rats exhibited a blunted response of the stimulus-elicited leukocyte rolling, which was associated with impairment of venular P-selectin expression as well as a decrease in the expression of sialyl Lewis X-like carbohydrates on circulating neutrophils. No significant differences were detected between the two strains not only in the surface CD11b/CD18 expression but also in the CD18-mediated adhesivity of neutrophils to intracellular adhesion molecule-1 transfectants in vitro. These results suggest that impairment of selectin-mediated leukocyte adhesion is an event responsible for disorders of inflammatory responses in spontaneously hypertensive rats. Images PMID:7560094

Suematsu, M; Suzuki, H; Tamatani, T; Iigou, Y; DeLano, F A; Miyasaka, M; Forrest, M J; Kannagi, R; Zweifach, B W; Ishimura, Y

1995-01-01

227

The relationship between serum adiponectin and inflammatory cytokines in obese Korean juveniles  

PubMed Central

Purpose Obesity is related to systemic inflammatory processes causing cardiovascular complications. Intercellular adhesion molecule-1 (ICAM-1), CD40 ligand (CD40L), P-selectin are newly described mediators of inflammation and have a significant effect in atherosclerosis. Adiponectin has shown anti-inflammatory effects in adults. The aim of this study was to evaluate the relationship between adiponectin and inflammatory mediators in children and adolescents. Methods Fifty children or adolescents, twenty two with a body mass index (BMI) over 95th percentile, and twenty eight with a BMI below 75th percentile were included in the study. Serum soluble ICAM-1 (sICAM-1), P-selectin, CD40L, lipid profiles, aspartate aminotransferase, alanine aminotransferase, glucose and insulin were measured to evaluate associations with adiponectin. Comparison of these variables was performed between the obese and the nonobese group. Results We found a adiponectin to be significant lower and sICAM-1 significant higher in the obese group compared to the nonobese group, but there were no significant differences in P-selectin and soluble CD40L. Adiponectin was negatively associated with ICAM-1 and P-selectin in the obese group. Conclusion Negative associations of adiponectin with ICAM-1 and P-selectin in obese children and adolescents suggest that serum adiponectin level may represent the inflammatory status. PMID:25653687

Byun, Sung Hwan; Kwon, Eun Byul

2014-01-01

228

Association between Endothelial Biomarkers and Arterial Elasticity in Young Adults – The CARDIA Study  

PubMed Central

Background Reduced arterial elasticity and endothelial dysfunction both may indicate early cardiovascular (CV) disease in young adults. Pulse waveform analysis estimates large (LAE) and small (SAE) artery elasticity noninvasively. We assessed the associations between LAE and SAE and markers of endothelial dysfunction and CV risk factors. Methods The Coronary Artery Risk Development in Young Adults (CARDIA) assessed arterial elasticity and other characteristics cross-sectionally in 389 men and 381 women aged 27–42 years in 1995 (CARDIA year 10) and circulating levels of P-selectin and soluble intercellular adhesion molecule 1 (sICAM-1) in 2000. We adjusted for variables included in the estimation of arterial elasticity (year 10 height, body mass index, age, heart rate, and blood pressure) and other year 10 characteristics. Results Mean adjusted SAE was 8.5 vs. 7.6 ml/mmHg ×100 in those with urine albumin/creatinine ratio ?4 vs. microalbuminuria (ratio > 25; ptrend =0.008). Mean LAE was 25.6 vs. 24.2 ml/mmHg ×10 in the lowest vs. highest quintile of P-selectin (ptrend =0.004). sICAM-1 was unrelated to either LAE or SAE. Plasma triglycerides were inversely related to LAE (ptrend =0.029). Cigarette smokers had lower SAE than nonsmokers (ptrend = 0.009). Conclusion In addition to smoking and triglycerides, biomarkers for endothelial dysfunction were associated with impaired LAE and SAE in young adults. PMID:19343081

Valappil, Narayanan I.; Jacobs, David R.; Duprez, Daniel A.; Gross, Myron D.; Arnett, Donna K.; Glasser, Stephen

2008-01-01

229

Increased plasma levels of BDNF and inflammatory markers in Alzheimer's disease.  

PubMed

Alzheimer's disease (AD) is the most common cause of dementia in the elderly. Neurotrophic factors and inflammatory markers may play considerable roles in AD. In this study we measured, through Enzyme-Linked Immunosorbent Assay, the plasma levels of brain derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF) and neuronal growth factor (NGF), as well as tumor necrosis factor-alpha soluble receptors, sTNFR1 and sTNFR2, and soluble intercellular adhesion molecule 1 (sICAM-1), in 50 AD patients, 37 patients with mild cognitive impairment (MCI) and 56 healthy elderly controls. BDNF levels, expressed as median and interquartile range, were higher for AD patients (2545.3, 1497.4-4153.4 pg/ml) compared to controls (1503.8, 802.3-2378.4 pg/ml), P < 0.001. sICAM-1 was also higher in AD patients. sTNFR1 levels were increased in AD when compared to controls and also to MCI. GDNF, NGF and sTNFR2 levels showed no significant differences among the studied groups. The increase in BDNF might reflect a compensatory mechanism against early neurodegeneration and seems to be related to inflammation. sTNFR1 appears to mark not only the inflammatory state but also differentiates between MCI and AD, which may be an additional tool for differentiating degrees of cognitive impairment. PMID:24576746

Faria, Mayara Chaves; Gonçalves, Gisele Santos; Rocha, Natália Pessoa; Moraes, Edgar Nunes; Bicalho, Maria Aparecida; Gualberto Cintra, Marco Túlio; Jardim de Paula, Jonas; José Ravic de Miranda, Luís Felipe; Clayton de Souza Ferreira, Alessandro; Teixeira, Antônio Lúcio; Gomes, Karina Braga; Carvalho, Maria das Graças; Sousa, Lirlândia P

2014-06-01

230

16 CFR 1500.133 - Extremely flammable contact adhesives; labeling.  

Code of Federal Regulations, 2011 CFR

...flammable contact adhesives; labeling. 1500...flammable contact adhesives; labeling. ...flammable contact adhesives, also known as contact bonding cements, when...one-half pint of contact adhesive and similar...

2011-01-01

231

16 CFR 1500.133 - Extremely flammable contact adhesives; labeling.  

Code of Federal Regulations, 2010 CFR

...flammable contact adhesives; labeling. 1500...flammable contact adhesives; labeling. ...flammable contact adhesives, also known as contact bonding cements, when...one-half pint of contact adhesive and similar...

2010-01-01

232

16 CFR 1500.133 - Extremely flammable contact adhesives; labeling.  

Code of Federal Regulations, 2012 CFR

...flammable contact adhesives; labeling. 1500...flammable contact adhesives; labeling. ...flammable contact adhesives, also known as contact bonding cements, when...one-half pint of contact adhesive and similar...

2012-01-01

233

16 CFR 1500.133 - Extremely flammable contact adhesives; labeling.  

Code of Federal Regulations, 2014 CFR

...flammable contact adhesives; labeling. 1500...flammable contact adhesives; labeling. ...flammable contact adhesives, also known as contact bonding cements, when...one-half pint of contact adhesive and similar...

2014-01-01

234

16 CFR 1500.133 - Extremely flammable contact adhesives; labeling.  

Code of Federal Regulations, 2013 CFR

...flammable contact adhesives; labeling. 1500...flammable contact adhesives; labeling. ...flammable contact adhesives, also known as contact bonding cements, when...one-half pint of contact adhesive and similar...

2013-01-01

235

Endothelin-1 plus oxidized low-density lipoprotein, but neither alone, increase human monocyte adhesion to endothelial cells.  

PubMed

Endothelin-1 is a potent vasoconstrictor and mitogenic peptide that is implicated in the atherosclerosis of apolipoprotein E-deficient mice and may promote atherogenesis in humans. We hypothesized that endothelin-1 might promote the adhesion of monocytes to endothelial cells, a key early event in atherosclerosis. We investigated the adhesion of primary human monocytes (isolated by elutriation) to human umbilical vein endothelial cell cultures after incubation with endothelin-1 (0.1 and 0.01 nM; approximately physiological concentrations), copper-oxidized low-density lipoprotein (LDL) (0.1 mg/ml) and a combination of the two. After a 4 h incubation with 0.1 or 0.01 nM endothelin-1 combined with oxidized LDL, adhesion was increased to 120+/-4% (P<0.001 compared with control) and 118+/-4% (P<0.002) respectively, whereas neither substance alone increased adhesion (92-104% of control values; not significant). Neither endothelin receptor A blockade nor co-incubation with anti-fibronectin antibody inhibited the pro-adhesive effects of endothelin-1 plus oxidized LDL (115+/-7% and 115+/-3% of control compared with 120+/-4% respectively; not significant). Endothelial cell expression of intercellular adhesion molecule-1, vascular adhesion molecule-1 and E-selectin were unchanged throughout the experiment. Therefore physiological concentrations of endothelin-1 and oxidized LDL may act synergistically to increase the adhesion of human monocytes to endothelial cells, contributing in part to the observed pro-atherogenic effects of endothelin-1. PMID:11724663

Langenfeld, M R; Nakhla, S; Death, A K; Jessup, W; Celermajer, D S

2001-12-01

236

?-Oryzanol reduces adhesion molecule expression in vascular endothelial cells via suppression of nuclear factor-?B activation.  

PubMed

?-Oryzanol (?-ORZ) is a mixture of phytosteryl ferulates purified from rice bran oil. In this study, we examined whether ?-ORZ represents a suppressive effect on the lipopolysaccharide (LPS)-induced adhesion molecule expression on vascular endothelium. Treatment with LPS elevated the mRNA expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin in bovine aortic endothelial cells (BAECs). Pretreatment with ?-ORZ dose-dependently decreased the LPS-mediated expression of these genes. Western blotting also revealed that pretreatment with ?-ORZ dose-dependently inhibited LPS-induced VCAM-1 expression in human umbilical vein endothelial cells. Consistently, pretreatment with ?-ORZ dose-dependently reduced LPS-induced U937 monocyte adhesion to BAECs. In immunofluorescence, LPS caused nuclear factor-?B (NF-?B) nuclear translocation in 40% of BAECs, which indicates NF-?B activation. Pretreatment with ?-ORZ, as well as its components (cycloartenyl ferulate, ferulic acid, or cycloartenol), dose-dependently inhibited LPS-mediated NF-?B activation. Collectively, our results suggested that ?-ORZ reduced LPS-mediated adhesion molecule expression through NF-?B inhibition in vascular endothelium. PMID:22401580

Sakai, Satoshi; Murata, Takahisa; Tsubosaka, Yoshiki; Ushio, Hideki; Hori, Masatoshi; Ozaki, Hiroshi

2012-04-01

237

Hot melt adhesive attachment pad  

NASA Technical Reports Server (NTRS)

A hot melt adhesive attachment pad for releasably securing distinct elements together is described which is particularly useful in the construction industry or a spatial vacuum environment. The attachment pad consists primarily of a cloth selectively impregnated with a charge of hot melt adhesive, a thermo-foil heater, and a thermo-cooler. These components are securely mounted in a mounting assembly. In operation, the operator activates the heating cycle transforming the hot melt adhesive to a substantially liquid state, positions the pad against the attachment surface, and activates the cooling cycle solidifying the adhesive and forming a strong, releasable bond.

Fox, R. L.; Frizzill, A. W.; Little, B. D.; Progar, D. J.; Coultrip, R. H.; Couch, R. H.; Gleason, J. R.; Stein, B. A.; Buckley, J. D.; St.clair, T. L. (inventors)

1984-01-01

238

Elastomer-toughened polyimide adhesives  

NASA Technical Reports Server (NTRS)

A study has been conducted to determine the effects of added elastomers on the Tg, thermal stability, adhesive strength, and fracture toughness of LARC-13, a high-temperature addition polyimide adhesive. Various butadiene/acrylonitrile and silicone elastomers were incorporated into the polyimide resin (1) as physical polyblends, and (2) by chemically reacting the elastomers with the polyimide backbone. Adhesive single lap-shear and T-peel strengths were measured before and after aging at elevated temperature. A tapered double-cantilever beam specimen was used to determine the fracture toughness of the elastomer-modified polyimide adhesives.

Saint Clair, A. K.; Saint Clair, T. L.

1980-01-01

239

All-trans-retinoic acid induces integrin-independent B-cell adhesion to ADAM disintegrin domains.  

PubMed

Cell adhesion is an integral aspect of immunity facilitating extravasation of immune cells during homing and activation. All -trans-Retinoic acid ( t-RA) regulates leukocyte differentiation, proliferation, and transmigration. However, the role of t-RA in immune cell adhesion is poorly defined. In this study, we evaluated the impact of t-RA and its metabolism on B and T cell adhesion. Specifically, we address the impact of t-RA on the adhesive properties of the human mature B and T cell lines RPMI 8866, Daudi and Jurkats. The effect of t-RA exposure on cell adhesion to vascular cell adhesion molecule-1 (VCAM-1), a well-established integrin counter receptor involved in immunity, and to nonconventional ADAM integrin ligands was assessed. We show for the first time that t-RA potently induces B cell adhesion in an integrin-independent manner to both VCAM-1 and select ADAM disintegrin domains. Using retinoid extraction and reverse-phase HPLC analysis, we identify the retinoid that is functionally responsible for this augmented adhesion. We also provide evidence that this novel t-RA adhesive response is not prototypical of lymphocytes since both Daudi and Jurkats do not alter their adhesive properties upon t-RA treatment. Further, the t-RA metabolic profiles between these lineages is distinct with 9- cis-retinoic acid being exclusively detected in Jurkat media. This study is the first to demonstrate that t-RA directly induces B cell adhesion in an integrin-independent manner and is not contingent upon t-RA metabolism. PMID:18355041

Bridges, Lance C; Lingo, Joshuah D; Grandon, Rachel A; Kelley, Melissa D

2008-04-15

240

UHU® adhesive holograms replication  

NASA Astrophysics Data System (ADS)

It is possible to apply a new recording material with high diffraction efficiency (of the order of 82.3%) to replicate computer phase or analogical holograms. This material is the all purpose adhesive UHU®. It is constituted by some components of polyvinyl, nitrates and some solvent agents; it is easily applied to any substrate. We record this material with heat generation by hand rubbing, using a mask (Kodalith® films) manufactured with lithographic techniques. The holographic replication is excellent on the new material UHU® adhesive, showing a phase modulation for refraction index and relief. This modulation is determined by the cured polymers process induced by friction, as pressure and temperature, with an anaerobic reaction. For copy of conventional holograms at high frequencies (holographic ranges), the diffraction efficiency parameter is in the neighborhood of 19.1% at first order or more, depending on diffraction efficiency of the pattern of the hologram. The hologram is elaborated in the absence of any development process and does not need to have carefully controlled conditions of the environment. Following this process, the hologram is obtained at standard atmospheric conditions of pressure and temperature.

Olivares-Pérez, A.; Toxqui-López, S.; Grijalva-Y-Ortiz, N.; Fuentes-Tapia, I.; Quintero-Romo, A.

2008-08-01

241

21 CFR 878.4380 - Drape adhesive.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Drape adhesive. 878.4380 Section 878.4380 Food... Surgical Devices § 878.4380 Drape adhesive. (a) Identification. A drape adhesive is a device intended to be placed on...

2010-04-01

242

21 CFR 878.4380 - Drape adhesive.  

Code of Federal Regulations, 2011 CFR

...2011-04-01 2011-04-01 false Drape adhesive. 878.4380 Section 878.4380 Food... Surgical Devices § 878.4380 Drape adhesive. (a) Identification. A drape adhesive is a device intended to be placed on...

2011-04-01

243

21 CFR 878.4380 - Drape adhesive.  

Code of Federal Regulations, 2014 CFR

...2014-04-01 2014-04-01 false Drape adhesive. 878.4380 Section 878.4380 Food... Surgical Devices § 878.4380 Drape adhesive. (a) Identification. A drape adhesive is a device intended to be placed on...

2014-04-01

244

21 CFR 878.4380 - Drape adhesive.  

Code of Federal Regulations, 2012 CFR

...2012-04-01 2012-04-01 false Drape adhesive. 878.4380 Section 878.4380 Food... Surgical Devices § 878.4380 Drape adhesive. (a) Identification. A drape adhesive is a device intended to be placed on...

2012-04-01

245

BLOOD LEUKOCYTE EXPRESSION OF LFA-1 AND INTRACELLULAR ADHESION MOLECULE-1 (ICAM-1) AFTER INHALATION OF ULTRAFINE CARBON PARTICLES. (R827354C003)  

EPA Science Inventory

The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

246

Trophoblast interactions with endothelial cells are increased by interleukin-1? and tumour necrosis factor ? and involve vascular cell adhesion molecule-1 and ?4?1  

Microsoft Academic Search

Interactions between fetal extravillous trophoblast cells and maternal uterine cells are of critical importance in successful placentation. In the first trimester, trophoblasts invade the uterine environment and reach the spiral arteries where they interact with vascular cells; however, little is known of the nature of these interactions. We have developed a fluorescent binding assay to investigate the contact between trophoblasts

J. E. Cartwright; G. Balarajah

2005-01-01

247

Dihydrotestosterone Promotes Vascular Cell Adhesion Molecule1 Expression in Male Human Endothelial Cells via a Nuclear Factor B-Dependent Pathway  

Microsoft Academic Search

There exists a striking gender difference in atherosclerotic vascular disease. For decades, estrogen was considered atheroprotective; however, an alternative is that androgen exposure in early life may predispose men to earlier athero- sclerosis. We recently demonstrated that the potent androgen, dihydrotestosterone (DHT), enhanced the binding of mono- cytes to the endothelium, a key early event in atherosclerosis, via increased expression

ALISON K. DEATH; KRISTINE C. Y. MCGRATH; MARK A. SADER; SHIRLEY NAKHLA; WENDY JESSUP; DAVID J. HANDELSMAN; DAVID S. CELERMAJER

2003-01-01

248

Transcriptional activation of the intercellular adhesion molecule 1 (CD54) gene by human T lymphotropic virus types I and II Tax is mediated through a palindromic response element.  

PubMed

In vitro infection of T cells with human T lymphotropic virus types I and II (HTLV-I and HTLV-II) resulted in constitutive expression of ICAM-1. Higher levels of ICAM-1 mRNA were expressed in HTLV-transformed cell lines (MT-2, MoT, C8166) when compared with uninfected T cell lines (A301). We demonstrate that this activation is conferred through a site on the ICAM-1 promoter that is activated in trans by the Tax protein of HTLV-I and HTLV-II. Enhanced promoter activity was detected when the ICAM-1 construct (-1162/+1) was transfected into HTLV-I-infected (MT-2), HTLV-II-infected (MoT, AI 1050), or an HTLV-I Tax-only-expressing (C8166) cell line as compared to the uninfected T cell line (A3.01). Cotransfection of the uninfected T cell line A3.01 with the ICAM construct along with Tax-I and Tax-II expression plasmid also resulted in increased promoter activity. Furthermore, experiments with deletion constructs of the ICAM-1 promoter region indicated that a region between -88 and -53 bp relative to the transcription start site is sufficient for Tax-inducible CAT expression. This segment includes an 11-bp palindromic segment (TTTCCGGGAAA) that has homology with the IFN-gamma and IL-6 response element. An 11-bp segment containing this regulatory region proved to be sufficient to confer Tax-I and Tax-II inducibility on a heterologous promoter (TK-CAT). Taken together these findings indicate that constitutive expression of ICAM-1 by HTLV-infected cells is influenced by the viral trans-activator protein Tax. This increased expression of ICAM-1 in response to the Tax protein may play an important role in the lymphoproliferation associated with HTLV infection. PMID:9359663

Owen, S M; Rudolph, D L; Dezzutti, C S; Shibata, N; Naik, S; Caughman, S W; Lal, R B

1997-11-01

249

Adequate Expression of Protective Immunity in the Absence of Granuloma Formation in Mycobacterium tuberculosis-Infected Mice with a Disruption in the Intracellular Adhesion Molecule 1 Gene  

Microsoft Academic Search

It remains unknown whether the expression of cell-mediated protective immunity and the capacity to mount a delayed-type hypersensitivity (DTH) reaction in tuberculosis infection represent two manifestations of a basic response or are dissociable events. In this study, we present data in favor of the latter hypothesis, by showing that tuberculosis infection in the lungs of mice possessing only a truncated

CHRISTINE M. JOHNSON; ANDREA M. COOPER; ANTHONY A. FRANK; IAN M. ORME

1998-01-01

250

New type LOC adhesive tapes  

Microsoft Academic Search

A new technology of the plastic package is offered in volume production of 16 Mb DRAM. This new package, called the LOC (Lead On Chip) structured package, is characterized by directly bonding the lead frame to the surface of the chip with a adhesive tape. This technology requires some new type packaging materials. Adhesive tape is the key material used

Y. Okugawa; T. Yoshida; T. Suzuki; H. Nakayoshi

1994-01-01

251

Severe adhesive small bowel obstruction.  

PubMed

Adhesive small bowel obstruction is a frequent cause of hospital admission. Water soluble contrast studies may have diagnostic and therapeutic value and avoid challenging demanding surgical operations, but if bowel ischemia is suspected, prompt surgical intervention is mandatory. A 58-year-old patient was operated for extensive adhesive small bowel obstruction after having had two previous laparotomies for colorectal surgery, and had a complex clinical course with multiple operations and several complications. Different strategies of management have been adopted, including non-operative management with the use of hyperosmolar water soluble contrast medium, multiple surgical procedures, total parenteral nutrition (TPN) support, and finally use of antiadherences icodextrin solution. After 2 years follow-up the patient was doing well without presenting recurrent episodes of adhesive small bowel obstruction. For patients admitted several times for adhesive small bowel obstruction, the relative risk of recurring obstruction increases in relation to the number of prior episodes. Several strategies for non-operative conservative management of adhesive small bowel obstruction have already addressed diagnostic and therapeutic value of hyperosmolar water soluble contrast. According to the most recent evidence-based guidelines, open surgery is the preferred method for surgical treatment of strangulating adhesive small bowel obstruction as well as after failed conservative management. Research interest and clinical evidence are increasing in adhesions prevention. Hyaluronic acid-carboxycellulose membrane and icodextrin may reduce incidence of adhesions. PMID:23054502

Di Saverio, Salomone; Catena, Fausto; Kelly, Michael D; Tugnoli, Gregorio; Ansaloni, Luca

2012-12-01

252

Adhesion Transition of Flexible Sheets  

E-print Network

Intermolecular forces are known to precipitate adhesion events between solid bodies. Inspired by a macro-scale experiment showing the hysteretic adhesion of a piece of flexible tape over a plastic substrate, we develop here a model of far-field dry adhesion between two flexible sheets interacting via a power-law potential. We show that phase transitions from unadhered to adhered states occur as dictated by a dimensionless bending parameter representing the ratio of interaction strength to bending stiffness. The order of the adhesion transitions, as well as their hysteretic nature, is shown to depend on the form of the interaction potential between the flexible sheets. When three or more sheets interact, additional geometrical considerations determine the hierarchical or sequential nature of the adhesion transitions.

Arthur A. Evans; Eric Lauga

2009-05-31

253

Hyaluronan-mediated cellular adhesion  

NASA Astrophysics Data System (ADS)

Many cells surround themselves with a cushioning halo of polysaccharides that is further strengthened and organized by proteins. In fibroblasts and chrondrocytes, the primary component of this pericellular matrix is hyaluronan, a large linear polyanion. Hyaluronan production is linked to a variety of disease, developmental, and physiological processes. Cells manipulate the concentration of hyaluronan and hyaluronan receptors for numerous activities including modulation of cell adhesion, cell motility, and differentiation. Recent investigations by identify hyaluronan's role in mediating early-stage cell adhesion. An open question is how the cell removes the 0.5-10 micron thick pericellular matrix to allow for further mature adhesion events requiring nanometer scale separations. In this investigation, holographic optical tweezers are used to study the adhesion and viscoelastic properties of chondrocytes' pericellular matrix. Ultimately, we aim to shed further light on the spatial and temporal details of the dramatic transition from micron to nanometer gaps between the cell and its adhesive substrate.

Curtis, Jennifer

2005-03-01

254

Universal adhesives: the next evolution in adhesive dentistry?  

PubMed

Every so often a new material, technique, or technological breakthrough spurs a paradigm shift in the way dentistry is practiced. The development and evolution of reliable enamel and dentin bonding agents is one such example. Indeed, the so-called "cosmetic revolution" in dentistry blossomed in large part due to dramatic advances in adhesive technology. It is the ability to bond various materials in a reasonably predictable fashion to both enamel and dentin substrates that enables dentists to routinely place porcelain veneers, direct and indirect composites, and a plethora of other restorative and esthetic materials. In fact, the longevity and predictability of many (if not most) current restorative procedures is wholly predicated on the dentist's ability to bond various materials to tooth tissues. Adhesive systems have progressed from the largely ineffective systems of the 1970s and early 1980s to the relatively successful total- and self-etching systems of today. The latest players in the adhesive marketplace are the so-called "universal adhesives." In theory, these systems have the potential to significantly simplify and expedite adhesive protocols and may indeed represent the next evolution in adhesive dentistry. But what defines a universal system, and are all these new systems truly "universal" and everything they are claimed to be? This article will examine the origin, chemistry, strengths, weaknesses, and clinical relevance of this new genre of dental adhesives. PMID:25822403

Alex, Gary

2015-01-01

255

Kindlin-3 regulates integrin activation and adhesion reinforcement of effector T cells  

PubMed Central

Activated T cells use very late antigen-4/?4?1 integrin for capture, rolling on, and firm adhesion to endothelial cells, and use leukocyte function-associated antigen-1/?L?2 integrin for subsequent crawling and extravasation. Inhibition of ?4?1 is sufficient to prevent extravasation of activated T cells and is successfully used to combat autoimmune diseases, such as multiple sclerosis. Here we show that effector T cells lacking the integrin activator Kindlin-3 extravasate and induce experimental autoimmune encephalomyelitis in mice immunized with autoantigen. In sharp contrast, adoptively transferred autoreactive T cells from Kindlin-3–deficient mice fail to extravasate into the naïve CNS. Mechanistically, autoreactive Kindlin-3–null T cells extravasate when the CNS is inflamed and the brain microvasculature expresses high levels of integrin ligands. Flow chamber assays under physiological shear conditions confirmed that Kindlin-3–null effector T cells adhere to high concentrations of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1, albeit less efficiently than WT T cells. Although these arrested T cells polarize and start crawling, only few remain firmly adherent over time. Our data demonstrate that the requirement of Kindlin-3 for effector T cells to induce ?4?1 and ?L?2 integrin ligand binding and stabilization of integrin–ligand bonds is critical when integrin ligand levels are low, but of less importance when integrin ligand levels are high. PMID:24089451

Moretti, Federico A.; Moser, Markus; Lyck, Ruth; Abadier, Michael; Ruppert, Raphael; Engelhardt, Britta; Fässler, Reinhard

2013-01-01

256

Suppression of adhesion molecule expression by phenanthrene-containing extract of bulbils of Chinese Yam in vascular smooth muscle cells through inhibition of MAPK, Akt and NF-?B.  

PubMed

Atherosclerosis is a chronic inflammatory disease associated with increased expression of adhesion molecules in vascular smooth muscle cells (VSMCs). The objective of this study was to examine the in vitro effects of extract from aerial Bulbil of Dioscorea batatas Decne (Db-Ex) on the ability to suppress the expression of adhesion molecules induced by TNF-?. We also identified bioactive components from a methanol extract. VSMCs pre-exposed to Db-Ex (10-100 ?g/ml) were stimulated with TNF-? (10 ng/ml). Preincubation of VSMCs for 2 h with Db-Ex dose-dependently inhibited TNF-?-induced adhesion of THP-1 monocytic cells and mRNA and protein expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). Db-Ex treatment decreased ROS production and the amount of phosphorylated form of p38, ERK, JNK and Akt in TNF-?-stimulated cells, suggesting that Db-Ex inhibits adhesion molecule expression possibly through MAPK and Akt regulation. Db-Ex also suppressed TNF-?-activation NK-?B. This effect was mediated through degradation of I?B? and nuclear translocation of the p65 subunit of NF-?B. These results suggest that Db-Ex inhibits monocyte adhesion and the TNF-?-mediated induction of adhesion molecules in VSMC by downregulating the MAPK/Akt/NF-?B signaling pathway, which may explain the ability of Db-Ex to suppress inflammation within the atherosclerotic lesion. PMID:22583650

Choi, Ko-Woon; Um, Sung Hee; Kwak, Jong-Hwan; Park, Hye-Jin; Kim, Kyung-Ho; Moon, Eun-Yi; Kwon, Soon-Tae; Pyo, Suhkneung

2012-08-01

257

Plasma inflammatory and vascular homeostasis biomarkers increase during human pregnancy but are not affected by oily fish intake.  

PubMed

The Salmon in Pregnancy Study investigated whether the increased consumption of (n-3) long-chain PUFA (LC-PUFA) from farmed Atlantic salmon affects immune function during pregnancy and atopic disease in neonates compared with a habitual diet low in oily fish. In this context, because the ingestion of (n-3) LC-PUFA may lower the concentrations of inflammatory biomarkers, we investigated whether the consumption of oily fish affects the levels of inflammatory cytokines and vascular adhesion factors during pregnancy. Pregnant women (n = 123) were randomly assigned to continue their habitual diet (control group, n = 61), which was low in oily fish, or to consume two 150-g salmon portions/wk (salmon group, n = 62; providing 3.45 g EPA plus DHA) from 20 wk of gestation until delivery. Plasma inflammatory cytokines and vascular adhesion factors were measured in maternal plasma samples. Inflammatory biomarkers, including IL-8, hepatocyte growth factor, and monocyte chemotactic protein, increased over the course of pregnancy (P < 0.001), whereas plasma matrix metalloproteinase 9, IL-6, TNF?, and nerve growth factor concentrations were not affected. Vascular homeostasis biomarkers soluble E-selectin, soluble vascular adhesion molecule-1, soluble intercellular adhesion molecule (sICAM)-1, and total plasminogen activator inhibitor-1 increased as pregnancy progressed (P < 0.001). The plasma sICAM-1 concentration was greater in the control group than in the salmon group at wk 20 (baseline) and 38 (P = 0.007) but there was no group x time interaction, and when baseline concentration was used as a covariate, the groups did not differ (P = 0.69). The remaining biomarkers analyzed were similar in both groups. Therefore, although some inflammatory and vascular homeostasis biomarkers change during pregnancy, they are not affected by the increased intake of farmed salmon. PMID:22623389

García-Rodríguez, Cruz E; Olza, Josune; Aguilera, Concepción M; Mesa, María D; Miles, Elizabeth A; Noakes, Paul S; Vlachava, Maria; Kremmyda, Lefkothea-Stella; Diaper, Norma D; Godfrey, Keith M; Calder, Philip C; Gil, Angel

2012-07-01

258

Chlorophyll-Related Compounds Inhibit Cell Adhesion and Inflammation in Human Aortic Cells  

PubMed Central

Abstract The objectives of this study were to investigate the effects of chlorophyll-related compounds (CRCs) and chlorophyll (Chl) a+b on inflammation in human aortic endothelial cells. Adhesion molecule expression and interleukin (IL)-8, nuclear factor (NF)-?B p65 protein, and NF-?B and activator protein (AP)-1 DNA binding were assessed. The effects of CRCs on inflammatory signaling pathways of signal transducers and activators of transcription 3 (STAT3) and mothers against decapentaplegic homolog 4, respectively induced by IL-6 and transforming growth factor (TGF)-?, in human aortic smooth muscle cells cultured in vitro were also investigated. HAECs were pretreated with 10 ?M of CRCs, Chl a+b, and aspirin (Asp) for 18?h followed by tumor necrosis factor (TNF)-? (2?ng/mL) for 6?h, and U937 cell adhesion was determined. TNF-?–induced monocyte-endothelial cell adhesion was significantly inhibited by CRCs. Moreover, CRCs and Chl a+b significantly attenuated vascular cell adhesion molecule-1, intercellular adhesion molecule-1, and IL-8 expressions. Treatments also significantly decreased in NF-?B expression, DNA binding, and AP-1 DNA binding by CRCs and Asp. Thus, CRCs exert anti-inflammatory effects through modulation of NF-?B and AP-1 signaling. Ten micromoles of CRCs and Asp upregulated the expression of mothers against decapentaplegic homolog 4 (Drosophila) (SMAD4) in the TGF-? receptor signaling pathway, and SMAD3/4 transcription activity was also increased. Ten micromoles of CRCs were able to potently inhibit STAT3-binding activity by repressing IL-6–induced STAT3 expression. Our results provide a potential mechanism that explains the anti-inflammatory activities of these CRCs. PMID:24066944

Lin, Kuan-Hung; Hsu, Ching-Yun; Huang, Ya-Ping; Lai, Jun-You; Hsieh, Wen-Bin; Huang, Meng-Yuan

2013-01-01

259

Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1.  

PubMed

T lymphocytes are highly motile and constantly reposition themselves between a free-floating vascular state, transient adhesion and migration in tissues. The regulation behind this unique dynamic behaviour remains unclear. Here we show that T cells have a cell surface mechanism for integrated regulation of motility and adhesion and that integrin ligands and CXCL12/SDF-1 influence motility and adhesion through this mechanism. Targeting cell surface-expressed low-density lipoprotein receptor-related protein 1 (LRP1) with an antibody, or blocking transport of LRP1 to the cell surface, perturbed the cell surface distribution of endogenous thrombospondin-1 (TSP-1) while inhibiting motility and potentiating cytoplasmic spreading on intercellular adhesion molecule 1 (ICAM-1) and fibronectin. Integrin ligands and CXCL12 stimulated motility and enhanced cell surface expression of LRP1, intact TSP-1 and a 130,000 MW TSP-1 fragment while preventing formation of a de-adhesion-coupled 110 000 MW TSP-1 fragment. The appearance of the 130 000 MW TSP-1 fragment was inhibited by the antibody that targeted LRP1 expression, inhibited motility and enhanced spreading. The TSP-1 binding site in the LRP1-associated protein, calreticulin, stimulated adhesion to ICAM-1 through intact TSP-1 and CD47. Shear flow enhanced cell surface expression of intact TSP-1. Hence, chemokines and integrin ligands up-regulate a dominant motogenic pathway through LRP1 and TSP-1 cleavage and activate an associated adhesion pathway through the LRP1-calreticulin complex, intact TSP-1 and CD47. This regulation of T-cell motility and adhesion makes pro-adhesive stimuli favour motile responses, which may explain why T cells prioritize movement before permanent adhesion. PMID:24877199

Talme, Toomas; Bergdahl, Eva; Sundqvist, Karl-Gösta

2014-06-01

260

Adhesion testing device  

NASA Technical Reports Server (NTRS)

The present invention provides a testing apparatus and method for testing the adhesion of a coating to a surface. The invention also includes an improved testing button or dolly for use with the testing apparatus and a self aligning button hook or dolly interface on the testing apparatus. According to preferred forms, the apparatus and method of the present invention are simple, portable, battery operated rugged, and inexpensive to manufacture and use, are readily adaptable to a wide variety of uses, and provide effective and accurate testing results. The device includes a linear actuator driven by an electric motor coupled to the actuator through a gearbox and a rotatable shaft. The electronics for the device are contained in the head section of the device. At the contact end of the device, is positioned a self aligning button hook, attached below the load cell located on the actuator shaft.

LaPeyronnie, Glenn M. (Inventor); Huff, Charles M. (Inventor)

2010-01-01

261

Epidural Lysis of Adhesions  

PubMed Central

As our population ages and the rate of spine surgery continues to rise, the use epidural lysis of adhesions (LOA) has emerged as a popular treatment to treat spinal stenosis and failed back surgery syndrome. There is moderate evidence that percutaneous LOA is more effective than conventional ESI for both failed back surgery syndrome, spinal stenosis, and lumbar radiculopathy. For cervical HNP, cervical stenosis and mechanical pain not associated with nerve root involvement, the evidence is anecdotal. The benefits of LOA stem from a combination of factors to include the high volumes administered and the use of hypertonic saline. Hyaluronidase has been shown in most, but not all studies to improve treatment outcomes. Although infrequent, complications are more likely to occur after epidural LOA than after conventional epidural steroid injections. PMID:24478895

Lee, Frank; Jamison, David E.; Hurley, Robert W.

2014-01-01

262

Epidural lysis of adhesions.  

PubMed

As our population ages and the rate of spine surgery continues to rise, the use epidural lysis of adhesions (LOA) has emerged as a popular treatment to treat spinal stenosis and failed back surgery syndrome. There is moderate evidence that percutaneous LOA is more effective than conventional ESI for both failed back surgery syndrome, spinal stenosis, and lumbar radiculopathy. For cervical HNP, cervical stenosis and mechanical pain not associated with nerve root involvement, the evidence is anecdotal. The benefits of LOA stem from a combination of factors to include the high volumes administered and the use of hypertonic saline. Hyaluronidase has been shown in most, but not all studies to improve treatment outcomes. Although infrequent, complications are more likely to occur after epidural LOA than after conventional epidural steroid injections. PMID:24478895

Lee, Frank; Jamison, David E; Hurley, Robert W; Cohen, Steven P

2014-01-01

263

Propulsion by directional adhesion  

NASA Astrophysics Data System (ADS)

The rough, hairy integument of water-walking arthropods is well known to be responsible for their water-repellency; we here consider its additional propulsive role. We demonstrate that the tilted flexible leg hairs of water-walking arthropods render the leg cuticle directionally anisotropic: contact lines advance most readily towards the leg tips. The dynamical role of the resulting unidirectional adhesion is explored, and yields new insight into the manner in which water-walking arthropods generate thrust, glide and leap from the free surface. We thus provide new rationale for the fundamental topological difference in the roughness on plants and insects, and suggest novel directions for biomimetic design of smart, hydrophobic surfaces.

Bush, John; Prakash, Manu

2008-03-01

264

Kindlins in FERM adhesion  

PubMed Central

The Kindlin family of intracellular proteins has recently emerged as key regulators of cellular functions and cell-matrix interactions. The 3 members of this family, Kindlin-1, -2, and -3, perform an essential role in activation of integrin adhesion receptors, and expression of at least 1 Kindlin paralog is required to enable integrin activation in physiologically relevant settings. In humans, deficiencies in Kindlin-3 lead to a number of abnormalities affecting hemostasis, the immune system, and bone function, whereas the lack of Kindlin-1 causes profound skin defects. The importance of Kindlins is underscored by the results of animal knockout studies, which clearly show the indispensable and nonredundant functions of all 3 Kindlins in development and normal physiology. This review discusses recent progress in the studies of Kindlin protein family, emphasizing newly identified functions and potential mechanisms underlying differential activities of the family members. PMID:20228270

Malinin, Nikolay L.; Plow, Edward F.

2010-01-01

265

Wet adhesion and adhesive locomotion of snails on anti-adhesive non-wetting surfaces.  

PubMed

Creating surfaces capable of resisting liquid-mediated adhesion is extremely difficult due to the strong capillary forces that exist between surfaces. Land snails use this to adhere to and traverse across almost any type of solid surface of any orientation (horizontal, vertical or inverted), texture (smooth, rough or granular) or wetting property (hydrophilic or hydrophobic) via a layer of mucus. However, the wetting properties that enable snails to generate strong temporary attachment and the effectiveness of this adhesive locomotion on modern super-slippy superhydrophobic surfaces are unclear. Here we report that snail adhesion overcomes a wide range of these microscale and nanoscale topographically structured non-stick surfaces. For the one surface which we found to be snail resistant, we show that the effect is correlated with the wetting response of the surface to a weak surfactant. Our results elucidate some critical wetting factors for the design of anti-adhesive and bio-adhesion resistant surfaces. PMID:22693563

Shirtcliffe, Neil J; McHale, Glen; Newton, Michael I

2012-01-01

266

Biomarkers and neurodevelopment in perinatally HIV-infected or exposed youth: a structural equation model analysis  

PubMed Central

Objective To examine the relationship between markers of vascular dysfunction and neurodevelopmental outcomes in perinatally HIV-infected (PHIV+) and perinatally HIV-exposed but uninfected (PHEU) youth. Design Cross-sectional design within a prospective, 15-site US-based cohort study. Methods Neurodevelopmental outcomes were evaluated in relation to nine selected vascular biomarkers in 342 youth (212 PHIV+, 130 PHEU). Serum levels were assessed for adiponectin, C-reactive protein (CRP), fibrinogen, interleukin-6 (IL-6), soluble vascular cell adhesion molecule-1 (sVCAM-1), E-selectin (sE-selectin), monocyte chemoattractant protein (sMCP-1), intercellular adhesion molecule-1 (sICAM-1), and P-selectin (sP-selectin). The Wechsler Intelligence Scale for Children-Fourth Edition (WISC-IV) was administered at entry, yielding a Full-Scale IQ score, and four index scores. Factor analysis was conducted to reduce the biomarkers to fewer factors with related biological roles. Structural equation models (SEMs) were used to measure associations between resulting factors and WISC-IV scores. Results Mean participant age was 11.4 years, 54% were female, 70% black. The nine biomarkers were clustered into three factor groups: F1 (fibrinogen, CRP, and IL-6); F2 (sICAM-1 and sVCAM-1); and F3 (MCP-1, sP-selectin, and sE-selectin). Adiponectin showed little correlation with any factor. SEMs revealed significant negative association of F1 with WISC-IV processing speed score in the total cohort. This effect remained significant after adjusting for HIV status and other potential confounders. A similar association was observed when restricted to PHIV+ participants in both unadjusted and adjusted SEMs. Conclusion Aggregate measures of fibrinogen, CRP, and IL-6 may serve as a latent biomarker associated with relatively decreased processing speed in both PHIV+ and PHEU youth. PMID:24670521

Kapetanovic, Suad; Griner, Ray; Zeldow, Bret; Nichols, Sharon; Leister, Erin; Gelbard, Harris A.; Miller, Tracie L.; Hazra, Rohan; Mendez, Armando J.; Malee, Kathleen; Kammerer, Betsy; Williams, Paige L.

2014-01-01

267

Accelerated receptor shedding inhibits kidney injury molecule-1 (KIM-1)-mediated efferocytosis.  

PubMed

Efficient clearance of apoptotic cells (efferocytosis) prevents inflammation and permits repair following tissue injury. Kidney injury molecule-1 (KIM-1) is a receptor for phosphatidylserine, an "eat-me" signal exposed on the surface of apoptotic cells that marks them for phagocytic clearance. KIM-1 is upregulated on proximal tubule epithelial cells (PTECs) during ischemic acute kidney injury (AKI), enabling efferocytosis by surviving PTECs. KIM-1 is spontaneously cleaved at its ectodomain region to generate a soluble fragment that serves a sensitive and specific biomarker for AKI, but the biological relevance of KIM-1 shedding is unknown. Here, we sought to determine how KIM-1 shedding might regulate efferocytosis. Using cells that endogenously and exogenously express KIM-1, we found that hydrogen peroxide-mediated oxidative injury or PMA treatment accelerated KIM-1 shedding in a dose-dependent manner. KIM-1 shedding was also accelerated when apoptotic cells were added. Accelerated shedding or the presence of excess soluble KIM-1 in the extracellular milieu significantly inhibited efferocytosis. We also identified that TNF-?-converting enzyme (TACE or ADAM17) mediates both the spontaneous and PMA-accelerated shedding of KIM-1. While accelerated shedding inhibited efferocytosis, we found that spontaneous KIM-1 cleavage does not affect the phagocytic efficiency of PTECs. Our results suggest that KIM-1 shedding is accelerated by worsening cellular injury, and excess soluble KIM-1 competitively inhibits efferocytosis. These findings may be important in AKI when there is severe cellular injury. PMID:24829508

Gandhi, Rushi; Yi, James; Ha, Jihyen; Shi, Hang; Ismail, Ola; Nathoo, Sahra; Bonventre, Joseph V; Zhang, Xizhong; Gunaratnam, Lakshman

2014-07-15

268

POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters  

PubMed Central

Specialized proteins in the plasma membrane, endoplasmic reticulum (ER), and mitochondria tightly regulate intracellular calcium. A unique mechanism called store-operated calcium entry is activated when ER calcium is depleted, serving to restore intra-ER calcium levels. An ER calcium sensor, stromal interaction molecule 1 (STIM1), translocates within the ER membrane upon store depletion to the juxtaplasma membrane domain, where it interacts with intracellular domains of a highly calcium-selective plasma membrane ion channel, Orai1. STIM1 gates Orai1, allowing calcium to enter the cytoplasm, where it repletes the ER store via calcium-ATPases pumps. Here, we performed affinity purification of Orai1 from Jurkat cells to identify partner of STIM1 (POST), a 10-transmembrane–spanning segment protein of unknown function. The protein is located in the plasma membrane and ER. POST-Orai1 binding is store depletion-independent. On store depletion, the protein binds STIM1 and moves within the ER to localize near the cell membrane. This protein, TMEM20 (POST), does not affect store-operated calcium entry but does reduce plasma membrane Ca2+ pump activity. Store depletion promotes STIM1–POST complex binding to smooth ER and plasma membrane Ca2+ ATPases (SERCAs and PMCAs, respectively), Na/K-ATPase, as well as to the nuclear transporters, importins-? and exportins. PMID:22084111

Krapivinsky, Grigory; Krapivinsky, Luba; Stotz, Stephanie C.; Manasian, Yunona; Clapham, David E.

2011-01-01

269

17?-Estradiol Inhibits Phosphorylation of Stromal Interaction Molecule 1 (STIM1) Protein  

PubMed Central

Sex plays a significant role in the development of lung diseases including asthma, cancer, chronic bronchitis, and cystic fibrosis. In cystic fibrosis, 17?-estradiol (E2) may inhibit store-operated Ca2+ entry (SOCE) to impinge upon airway secretions, leaving females at greater risk of contracting lung infections. Stromal interaction molecule 1 (STIM1)-mediated SOCE is essential for cell homeostasis and regulates numerous processes including cell proliferation, smooth muscle contraction, and secretion. E2 can signal nongenomically to modulate Ca2+ signaling, but little is known of the underlying mechanisms. We found that E2 exposure inhibited STIM1 translocation in airway epithelia, preventing SOCE. This correlated with a decrease in STIM1-STIM1 FRET and STIM1 mobility in E2-exposed HEK293T cells co-expressing estrogen receptor ?. We also examined the role of STIM1 phosphorylation in E2-mediated inhibition of STIM1 mobility. STIM1 is basally phosphorylated at serine 575, which is required for SOCE. Exposure to E2 significantly decreased STIM1 serine phosphorylation. Mutating serine 575 to an alanine blocked STIM1 phosphorylation, reduced basal STIM1 mobility, and rendered STIM1 insensitive to E2. These data indicate that E2 can signal nongenomically by inhibiting basal phosphorylation of STIM1, leading to a reduction in SOCE. PMID:24114840

Sheridan, John T.; Gilmore, Rodney C.; Watson, Michael J.; Archer, Christopher B.; Tarran, Robert

2013-01-01

270

Kidney injury molecule-1 is an early biomarker of cadmium nephrotoxicity  

PubMed Central

Cadmium (Cd) exposure results in injury to the proximal tubule characterized by polyuria and proteinuria. Kidney injury molecule-1 (Kim-1) is a transmembrane glycoprotein not normally detected in the mature kidney, but is upregulated and shed into the urine following nephrotoxic injury. In this study, we determine if Kim-1 might be a useful early biomarker of Cd nephrotoxicity. Male Sprague-Dawley rats were given daily injections of Cd for up to 12 weeks. Weekly urine samples were analyzed for Kim-1, protein, creatinine, metallothionein, and Clara cell protein CC-16. Significant levels of Kim-1 were detected in the urine by 6 weeks and continued to increase throughout the treatment period. This appearance of Kim-1 occurred 4-5 weeks before the onset of proteinuria, and 1-3 weeks before the appearance of metallothionein and CC-16. Higher doses of Cd gave rise to higher Kim-1 excretion. Reverse transcriptase-polymerase chain reaction (RT-PCR) expression analysis showed that Kim-1 transcript levels were increased after 6 weeks at the low dose of Cd. Immunohistochemical analysis showed that Kim-1 was present in proximal tubule cells of the Cd-treated rats. Our results suggest that Kim-1 may be a useful biomarker of early stages of Cd-induced proximal tubule injury. PMID:17687258

Prozialeck, WC; Vaidya, VS; Liu, J; Waalkes, MP; Edwards, JR; Lamar, PC; Bernard, AM; Dumont, X; Bonventre, JV

2009-01-01

271

Adhesively-bonded joints and repairs in metallic alloys, polymers and composite materials: Adhesives, adhesion theories and surface pretreatment  

Microsoft Academic Search

In the present paper, the following topics are reviewed in detail: (a) the available adhesives, as well as their recent advances, (b) thermodynamic factors affecting the surface pretreatments including adhesion theories, wettability, surface energy, (c) bonding mechanisms in the adhesive joints, (d) surface pretreatment methods for the adhesively bonded joints, and as well as their recent advances, and (e) combined

A. Baldan

2004-01-01

272

Interfacial adhesion of carbon fibers  

NASA Technical Reports Server (NTRS)

Relative adhesion strengths between AS4, AS1, and XAS carbon fibers and thermoplastic polymers were determined using the embedded single filament test. Polymers studied included polycarbonate, polyphenylene oxide, polyetherimide, polysulfone, polyphenylene oxide blends with polystyrene, and polycarbonate blends with a polycarbonate polysiloxane block copolymer. Fiber surface treatments and sizings improved adhesion somewhat, but adhesion remained well below levels obtained with epoxy matrices. An explanation for the differences between the Hercules and Grafil fibers was sought using X ray photon spectroscopy, wetting, scanning electron microscopy and thermal desorption analysis.

Bascom, Willard D.

1987-01-01

273

Adhesives from modified soy protein  

DOEpatents

The, present invention provides useful adhesive compositions having similar adhesive properties to conventional UF and PPF resins. The compositions generally include a protein portion and modifying ingredient portion selected from the group consisting of carboxyl-containing compounds, aldehyde-containing compounds, epoxy group-containing compounds, and mixtures thereof. The composition is preferably prepared at a pH level at or near the isoelectric point of the protein. In other preferred forms, the adhesive composition includes a protein portion and a carboxyl-containing group portion.

Sun, Susan (Manhattan, KS); Wang, Donghai (Manhattan, KS); Zhong, Zhikai (Manhattan, KS); Yang, Guang (Shanghai, CN)

2008-08-26

274

Photovoltaic module with adhesion promoter  

DOEpatents

Photovoltaic modules with adhesion promoters and methods for fabricating photovoltaic modules with adhesion promoters are described. A photovoltaic module includes a solar cell including a first surface and a second surface, the second surface including a plurality of interspaced back-side contacts. A first glass layer is coupled to the first surface by a first encapsulating layer. A second glass layer is coupled to the second surface by a second encapsulating layer. At least a portion of the second encapsulating layer is bonded directly to the plurality of interspaced back-side contacts by an adhesion promoter.

2013-10-08

275

Wear mechanism based on adhesion  

NASA Technical Reports Server (NTRS)

Various concepts concerning wear mechanisms and deformation behavior observed in the sliding wear track are surveyed. The mechanisms for wear fragment formation is discussed on the basis of adhesion. The wear process under unlubricated sliding conditions is explained in relation to the concept of adhesion at the interface during the sliding process. The mechanism for tearing away the surface layer from the contact area and forming the sliding track contour is explained by assuming the simplified process of material removal based on the adhesion theory.

Yamamoto, T.; Buckley, D. H.

1982-01-01

276

Adhesive Exocrine Glands in Insects: Morphology, Ultrastructure, and Adhesive Secretion  

Microsoft Academic Search

\\u000a A literature survey is provided summarizing the available information on exocrine epidermal glands that produce adhesive secretions\\u000a in insects. The focus is on both the ultrastructure of the gland cells and the identity and function of the chemical secretion\\u000a produced by them. Insects employ adhesives for various functions such as tarsal attachment during locomotion, resisting external\\u000a detachment forces, mating, phoresy

Oliver Betz

277

Surface Energy and Adhesion Studies on Acrylic Pressure Sensitive Adhesives  

Microsoft Academic Search

The surface energy and adhesion dynamics of pressure sensitive adhesives-like networks (PSA-LNs) as mimics for PSAs were studied using JKR-based contact mechanics and peel tests. Acrylic acid (AA) was co-polymerized with 2-ethyl hexyl acrylate (2-EHA) and 1,6-hexane diol diacrylate (HDDA) to create PSA-LNs. The measured surface energy (27 to 31 mJ\\/m) was sensible as surmised from their structure. Acrylic acid

Lihua Li; Matthew Tirrell; Gary A. Korba; Alphonsus V. Pocius

2001-01-01

278

Classification of OPP adhesive tapes according to pyrogram of adhesives  

Microsoft Academic Search

Pressure sensitive adhesives (PSAs) of colorless and transparent oriented polypropylene (OPP) adhesive tapes were analyzed by pyrolysis\\/gas chromatography\\/mass spectrometry (Py\\/GC\\/MS). The PSAs were acrylic and rubber-based PSAs and the tapes were classified according to total ion current (TIC) chromatograms of the PSAs. The main pyrolyzates of the acrylic PSAs were decomposition products of monomers, monomers, dimmers and trimers. Those of

Y. Kumooka

2011-01-01

279

THE GLUING OF WOOD WITH SYNTHETIC ADHESIVES  

Microsoft Academic Search

The bonding of wood by means of glue has been practised for many centuries.Adhesion between an adhesive and wood is the result of unbalanced secondary valency forces (Van der Waal's forces) present on the interfaces. It is fundamental to good adhesion that the adhesive must (a) wet the surface it is required to adhere to and (b) penetrate the wood

G. Klein

1964-01-01

280

Viscoelastic Analysis of an Adhesive Tubular Joint  

Microsoft Academic Search

The investigations so far available with regard to stress analysis of adhesive joints assume that the adhesive is elastic. In the present analysis the time dependent properties of the adhesive are taken into account by assuming that the adhesive is viscoelastic. The viscoelastic analysis of a tubular joint has been attempted using a prony series fitting for the relaxation modulus

R. S. Alwar; Y. R. Nagaraja

1976-01-01

281

International Journal of Adhesion & Adhesives 20 (1999) 5970 International Journal of  

E-print Network

has been a popular method for measuring the fracture toughness of adhesive bonds and delamination adhesive bonds [4] and laminate delamination [5]. The same specimen geometry with modified loading methodsInternational Journal of Adhesion & Adhesives 20 (1999) 59­70 International Journal of Adhesion

Nairn, John A.

282

Seafood delicacy makes great adhesive  

SciTech Connect

Technology from Mother Nature is often hard to beat, so Idaho National Laboratory scientistsgenetically analyzed the adhesive proteins produced by blue mussels, a seafood delicacy. Afterobtaining full-length DNA sequences encoding these proteins, reprod

Idaho National Laboratory - Frank Roberto, Heather Silverman

2008-03-26

283

Seafood delicacy makes great adhesive  

ScienceCinema

Technology from Mother Nature is often hard to beat, so Idaho National Laboratory scientistsgenetically analyzed the adhesive proteins produced by blue mussels, a seafood delicacy. Afterobtaining full-length DNA sequences encoding these proteins, reprod

Idaho National Laboratory - Frank Roberto, Heather Silverman

2010-01-08

284

A Potential Role for Plasma Uric Acid in the Endothelial Pathology of Plasmodium falciparum malaria  

PubMed Central

Background Inflammatory cytokinemia and systemic activation of the microvascular endothelium are central to the pathogenesis of Plasmodium falciparum malaria. Recently, ‘parasite-derived’ uric acid (UA) was shown to activate human immune cells in vitro, and plasma UA levels were associated with inflammatory cytokine levels and disease severity in Malian children with malaria. Since UA is associated with endothelial inflammation in non-malaria diseases, we hypothesized that elevated UA levels contribute to the endothelial pathology of P. falciparum malaria. Methodology/Principal Findings We measured levels of UA and soluble forms of intercellular adhesion molecule-1 (sICAM-1), vascular cell adhesion molecule-1 (sVCAM-1), E-selectin (sE-Selectin), thrombomodulin (sTM), tissue factor (sTF) and vascular endothelial growth factor (VEGF) in the plasma of Malian children aged 0.5–17 years with uncomplicated malaria (UM, n?=?487) and non-cerebral severe malaria (NCSM, n?=?68). In 69 of these children, we measured these same factors once when they experienced a malaria episode and twice when they were healthy (i.e., before and after the malaria transmission season). We found that levels of UA, sICAM-1, sVCAM-1, sE-Selectin and sTM increase during a malaria episode and return to basal levels at the end of the transmission season (p<0.0001). Plasma levels of UA and these four endothelial biomarkers correlate with parasite density and disease severity. In children with UM, UA levels correlate with parasite density (r?=?0.092, p?=?0.043), sICAM-1 (r ?=?0.255, p<0.0001) and sTM (r?=?0.175, p?=?0.0001) levels. After adjusting for parasite density, UA levels predict sTM levels. Conclusions/Significance Elevated UA levels may contribute to malaria pathogenesis by damaging endothelium and promoting a procoagulant state. The correlation between UA levels and parasite densities suggests that parasitized erythrocytes are one possible source of excess UA. UA-induced shedding of endothelial TM may represent a novel mechanism of malaria pathogenesis, in which activated thrombin induces fibrin deposition and platelet aggregation in microvessels. This protocol is registered at clinicaltrials.gov (NCT00669084). PMID:23349902

Mita-Mendoza, Neida K.; van de Hoef, Diana L.; Lopera-Mesa, Tatiana M.; Doumbia, Saibou; Konate, Drissa; Doumbouya, Mory; Gu, Wenjuan; Anderson, Jennifer M.; Santos-Argumedo, Leopoldo; Rodriguez, Ana; Fay, Michael P.; Diakite, Mahamadou; Long, Carole A.; Fairhurst, Rick M.

2013-01-01

285

Investigation of organic adhesives for hybrid microcircuits  

NASA Technical Reports Server (NTRS)

The properties of organic adhesives were investigated to acquire information for a guideline document regarding the selection of adhesives for use in high reliability hybrid microcircuits. Specifically, investigations were made of (1) alternate methods for determining the outgassing of cured adhesives, (2) effects of long term aging at 150 C on the electrical properties of conductive adhesives, (3) effects of shelf life age on adhesive characteristics, (4) bond strengths of electrically conductive adhesives on thick film gold metallization, (5) a copper filled adhesive, (6) effects of products outgassed from cured adhesives on device electrical parameters, (7) metal migration from electrically conductive adhesives, and (8) ionic content of electrically insulative adhesives. The tests performed during these investigations are described, and the results obtained are discussed.

Perkins, K. L.; Licari, J. J.

1975-01-01

286

Adhesive interactions between vesicles in the strong adhesion limit  

PubMed Central

We consider the adhesive interaction energy between a pair of vesicles in the strong adhesion limit, in which bending forces play a negligible role in determining vesicle shape compared to forces due to membrane stretching. Although force-distance or energy distance relationships characterizing adhesive interactions between fluid bilayers are routinely measured using the surface forces apparatus, the atomic force microscope and the biomembrane force probe, the interacting bilayers in these methods are supported on surfaces (e.g. mica sheet) and cannot be deformed. However, it is known that in a suspension, vesicles composed of the same bilayer can deform by stretching or bending, and can also undergo changes in volume. Adhesively interacting vesicles can thus form flat regions in the contact zone, which will result in an enhanced interaction energy as compared to rigid vesicles. The focus of this paper is to examine the magnitude of the interaction energy between adhesively interacting, deformed vesicles relative to free, undeformed vesicles as a function of the intervesicle separation. The modification of the intervesicle interaction energy due to vesicle deformability can be calculated knowing the undeformed radius of the vesicles, R0, the bending modulus kb, the area expansion modulus Ka, and the adhesive minimum WP(0) and separation DP(0) in the energy of interaction between two flat bilayers, which can be obtained from the force-distance measurements made using the above supported-bilayer methods. For vesicles with constant volumes, we show that adhesive potentials between non-deforming bilayers such as ?WP(0)??5×10?4mJ/m2, which are ordinarily considered weak in colloidal physics literature, can result in significantly deep (>10×) energy minima due to increase in vesicle area and flattening in the contact region. If the osmotic expulsion of water across the vesicles driven by the tense, stretched membrane in the presence of an osmotically active solute is also taken into account, the vesicles can undergo additional deformation (flattening), which further enhances the adhesive interaction between them. Finally, equilibration of ions and solutes due to the concentration differences created by the osmotic exchange of water can lead to further enhancement of the adhesion energy. Our result of the progressively increasing adhesive interaction energy between vesicles in above regimes could explain why suspensions of very weakly attractive vesicles may undergo flocculation and eventual instability due to separation of vesicles from the suspending fluid by gravity. The possibility of such an instability is an extremely important issue for concentrated vesicle-based products and applications such as fabric softeners, hair therapeutics and drug delivery. PMID:21128653

Ramachandran, Arun; Anderson, Travers H.; Leal, L. Gary; Israelachvili, Jacob N.

2010-01-01

287

Adhesive interactions between vesicles in the strong adhesion limit.  

PubMed

We consider the adhesive interaction energy between a pair of vesicles in the strong adhesion limit, in which bending forces play a negligible role in determining vesicle shape compared to forces due to membrane stretching. Although force?distance or energy?distance relationships characterizing adhesive interactions between fluid bilayers are routinely measured using the surface forces apparatus, the atomic force microscope, and the biomembrane force probe, the interacting bilayers in these methods are supported on surfaces (e.g., mica sheet) and cannot be deformed. However, it is known that, in a suspension, vesicles composed of the same bilayer can deform by stretching or bending, and can also undergo changes in volume. Adhesively interacting vesicles can thus form flat regions in the contact zone, which will result in an enhanced interaction energy as compared to rigid vesicles. The focus of this paper is to examine the magnitude of the interaction energy between adhesively interacting, deformed vesicles relative to free, undeformed vesicles as a function of the intervesicle separation. The modification of the intervesicle interaction energy due to vesicle deformability can be calculated knowing the undeformed radius of the vesicles, R0, the bending modulus, k(b), the area expansion modulus, k(a), and the adhesive minimum, W(P)(0), and separation, D(P)(0), in the energy of interaction between two flat bilayers, which can be obtained from the force?distance measurements made using the above supported-bilayer methods. For vesicles with constant volumes, we show that adhesive potentials between nondeforming bilayers such as |W(P)(0)| 5 × 10(?4) mJ/m2, which are ordinarily considered weak in the colloidal physics literature, can result in significantly deep (>10×) energy minima due to increase in vesicle area and flattening in the contact region. If the osmotic expulsion of water across the vesicles driven by the tense, stretched membrane in the presence of an osmotically active solute is also taken into account, the vesicles can undergo additional deformation (flattening), which further enhances the adhesive interaction between them. Finally, equilibration of ions and solutes due to the concentration differences created by the osmotic exchange of water can lead to further enhancement of the adhesion energy. Our result of the progressively increasing adhesive interaction energy between vesicles in the above regimes could explain why suspensions of very weakly attractive vesicles may undergo flocculation and eventual instability due to separation of vesicles from the suspending fluid by gravity. The possibility of such an instability is an extremely important issue for concentrated vesicle-based products and applications such as fabric softeners, hair therapeutics and drug delivery. PMID:21128653

Ramachandran, Arun; Anderson, Travers H; Leal, L Gary; Israelachvili, Jacob N

2011-01-01

288

Fibronectin and Other Adhesive Glycoproteins  

Microsoft Academic Search

\\u000a Cells adhere to the extracellular matrix through interaction with adhesive extracellular matrix glycoproteins, including fibronectin,\\u000a laminins, vitronectin, thrombospondins, tenascins, entactins (or nidogens), nephronectin, fibrinogen, and others. Most adhesive\\u000a glycoproteins bind cells through cell surface integrin receptors in conjunction with other cell surface receptors, such as\\u000a dystroglycans and syndecans, and interact with other extracellular matrix proteins to form an intensive matrix

Jielin Xu; Deane Mosher

289

Fibrillar Adhesive for Climbing Robots  

NASA Technical Reports Server (NTRS)

A climbing robot needs to use its adhesive patches over and over again as it scales a slope. Replacing the adhesive at each step is generally impractical. If the adhesive or attachment mechanism cannot be used repeatedly, then the robot must carry an extra load of this adhesive to apply a fresh layer with each move. Common failure modes include tearing, contamination by dirt, plastic deformation of fibers, and damage from loading/ unloading. A gecko-like fibrillar adhesive has been developed that has been shown useful for climbing robots, and may later prove useful for grasping, anchoring, and medical applications. The material consists of a hierarchical fibrillar structure that currently contains two levels, but may be extended to three or four levels in continuing work. The contacting level has tens of thousands of microscopic fibers made from a rubberlike material that bend over and create intimate contact with a surface to achieve maximum van der Waals forces. By maximizing the real area of contact that these fibers make and minimizing the bending energy necessary to achieve that contact, the net amount of adhesion has been improved dramatically.

Pamess, Aaron; White, Victor E.

2013-01-01

290

Lymphocyte Adhesion to Candida albicans  

PubMed Central

Adherence of lymphocytes to the fungus is the first step in the direct lymphocyte-mediated antifungal effect against Candida albicans. In this study we identified macrophage-1 antigen (Mac-1) (CD11b/CD18, ?M/?2) as the lymphocyte surface structure responsible for the adhesion of activated lymphocytes to the hyphal form of the fungus. Antibodies specific for epitopes of the ?-subunit (CD11b) and the ?2-subunit (CD18) of Mac-1 were shown to completely eliminate lymphocyte adhesion to C. albicans hyphae. Lymphocyte adhesion to C. albicans was also inhibited significantly by known ligands of Mac-1, including the extracellular matrix proteins laminin and fibrinogen, as well as engineered peptides containing arginine-glycine-aspartic acid sequences and the disintegrin echistatin. N-Acetyl-d-glucosamine and ?-glucan, which inhibit Mac-1-mediated adhesion to the yeast, blocked lymphocyte adhesion to hyphae. NIH 3T3 fibroblast transfectants expressing human CD11b/CD18 bound to C. albicans, and their binding was inhibited by antibodies specific for CD11b/CD18. Finally, antibodies specific for CD11b/CD18 effectively inhibited the capacity of activated lymphocytes to have an antifungal effect against hyphae. Our results clearly identify Mac-1 (CD11b/CD18) as the lymphocyte surface structure that mediates activated lymphocyte adhesion to C. albicans and the resultant antifungal effect of the lymphocytes. PMID:11796578

Forsyth, Christopher B.; Mathews, Herbert L.

2002-01-01

291

Effects of nitric oxide-releasing nonsteroidal anti-inflammatory drugs (NONO-NSAIDs) on melanoma cell adhesion  

SciTech Connect

A new class of nitric oxide (NO•)-releasing nonsteroidal anti-inflammatory drugs (NONO-NSAIDs) were developed in recent years and have shown promising potential as NSAID substitutes due to their gentle nature on cardiovascular and gastrointestinal systems. Since nitric oxide plays a role in regulation of cell adhesion, we assessed the potential use of NONO-NSAIDs as anti-metastasis drugs. In this regard, we compared the effects of NONO-aspirin and a novel NONO-naproxen to those exerted by their respective parent NSAIDs on avidities of human melanoma M624 cells. Both NONO-NSAIDs, but not the corresponding parent NSAIDs, reduced M624 adhesion on vascular cellular adhesion molecule-1 (VCAM-1) by 20–30% and fibronectin by 25–44% under fluid flow conditions and static conditions, respectively. Only NONO-naproxen reduced (? 56%) the activity of ?1 integrin, which binds to ?4 integrin to form very late antigen-4 (VLA-4), the ligand of VCAM-1. These results indicate that the diazeniumdiolate (NO•)-donor moiety is critical for reducing the adhesion between VLA-4 and its ligands, while the NSAID moiety can impact the regulation mechanism of melanoma cell adhesion. -- Highlights: ? NONO-naproxen, a novel nitric oxide-releasing NSAID, was synthesized. ? NONO-NSAIDs, but not their parent NSAIDs, reduced melanoma adhesion. ? NONO-naproxen, but not NONO-aspirin and NSAIDs, reduced activity of ?1 integrin.

Cheng, Huiwen [Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (United States) [Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (United States); Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701 (United States); Mollica, Molly Y.; Lee, Shin Hee [Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (United States)] [Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (United States); Wang, Lei [Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (United States) [Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (United States); Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701 (United States); Velázquez-Martínez, Carlos A., E-mail: velazque@ualberta.ca [Chemistry Section, Laboratory of Comparative Carcinogenesis and Basic Research Program, SAIC-Frederick Inc., National Cancer Institute at Frederick, Frederick, MD 21702 (United States); Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton Alberta, Canada T6G 2N8 (Canada); Wu, Shiyong, E-mail: wus1@ohio.edu [Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (United States) [Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (United States); Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701 (United States)

2012-10-15

292

Adhesion of Elastic Spheres  

NASA Astrophysics Data System (ADS)

Bradley (1932) showed that if two rigid spheres of radii R1 and R2 are placed in contact, they will adhere with a force 2? R? ? , where R is the equivalent radius R1R2/(R1 + R2) and ? ? is the surface energy or 'work of adhesion' (equal to ? 1 + ? 2 - ? 12). Subsequently Johnson et al. (1971) (JKR theory) showed by a Griffith energy argument (assuming that contact over a circle of radius a introduces a surface energy -? a2? ? ) how the Hertz equations for the contact of elastic spheres are modifed by surface energy, and showed that the force needed to separate the spheres is equal to (3/2)? R? ? , which is independent of the elastic modulus and so appears to be universally applicable and therefore to conflict with Bradley's answer. The discrepancy was explained by Tabor (1977), who identified a parameter ? equiv R1/3? ? 2/3/E* 2/3? governing the transition from the Bradley pull-off force 2? R? ? to the JKR value (3/2)? R? ? . Subsequently Muller et al. (1980) performed a complete numerical solution in terms of surface forces rather than surface energy, (combining the Lennard-Jones law of force between surfaces with the elastic equations for a half-space), and confirmed that Tabor's parameter does indeed govern the transition. The numerical solution is repeated more accurately and in greater detail, confirming the results, but showing also that the load-approach curves become S-shaped for values of ? greater than one, leading to jumps into and out of contact. The JKR equations describe the behaviour well for values of ? of 3 or more, but for low values of ? the simple Bradley equation better describes the behaviour under negative loads.

Greenwood, J. A.

1997-06-01

293

NADPH oxidase and lipid raft-associated redox signaling are required for PCB153-induced upregulation of cell adhesion molecules in human brain endothelial cells  

SciTech Connect

Exposure to persistent organic pollutants, such as polychlorinated biphenyls (PCBs), can lead to chronic inflammation and the development of vascular diseases. Because cell adhesion molecules (CAMs) of the cerebrovascular endothelium regulate infiltration of inflammatory cells into the brain, we have explored the molecular mechanisms by which ortho-substituted polychlorinated biphenyls (PCBs), such as PCB153, can upregulate CAMs in brain endothelial cells. Exposure to PCB153 increased expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), as well as elevated adhesion of leukocytes to brain endothelial cells. These effects were impeded by inhibitors of EGFR, JAKs, or Src activity. In addition, pharmacological inhibition of NADPH oxidase or disruption of lipid rafts by cholesterol depleting agents blocked PCB153-induced phosphorylation of JAK and Src kinases and upregulation of CAMs. In contrast, silencing of caveolin-1 by siRNA interference did not affect upregulation of ICAM-1 and VCAM-1 in brain endothelial cells stimulated by PCB153. Results of the present study indicate that lipid raft-dependent NADPH oxidase/JAK/EGFR signaling mechanisms regulate the expression of CAMs in brain endothelial cells and adhesion of leukocytes to endothelial monolayers. Due to its role in leukocyte infiltration, induction of CAMs may contribute to PCB-induced cerebrovascular disorders and neurotoxic effects in the CNS.

Eum, Sung Yong [Molecular Neuroscience and Vascular Biology Laboratory, Department of Neurosurgery, University of Kentucky, Lexington, KY 40536 (United States)], E-mail: sungyong.eum@uky.edu; Andras, Ibolya [Molecular Neuroscience and Vascular Biology Laboratory, Department of Neurosurgery, University of Kentucky, Lexington, KY 40536 (United States); Hennig, Bernhard [College of Agriculture, University of Kentucky, Lexington, KY 40536 (United States); Toborek, Michal [Molecular Neuroscience and Vascular Biology Laboratory, Department of Neurosurgery, University of Kentucky, Lexington, KY 40536 (United States)

2009-10-15

294

Capillarity-based switchable adhesion  

PubMed Central

Drawing inspiration from the adhesion abilities of a leaf beetle found in nature, we have engineered a switchable adhesion device. The device combines two concepts: The surface tension force from a large number of small liquid bridges can be significant (capillarity-based adhesion) and these contacts can be quickly made or broken with electronic control (switchable). The device grabs or releases a substrate in a fraction of a second via a low-voltage pulse that drives electroosmotic flow. Energy consumption is minimal because both the grabbed and released states are stable equilibria that persist with no energy added to the system. Notably, the device maintains the integrity of an array of hundreds to thousands of distinct interfaces during active reconfiguration from droplets to bridges and back, despite the natural tendency of the liquid toward coalescence. We demonstrate the scaling of adhesion strength with the inverse of liquid contact size. This suggests that strengths approaching those of permanent bonding adhesives are possible as feature size is scaled down. In addition, controllability is fast and efficient because the attachment time and required voltage also scale down favorably. The device features compact size, no solid moving parts, and is made of common materials. PMID:20133725

Vogel, Michael J.; Steen, Paul H.

2010-01-01

295

Adhesive contact of elastomers: effective adhesion energy and creep function  

E-print Network

For the adhesive contact of elastomers, we propose expressions to quantify the impact of viscoelastic response on effective adhesion energy as a function of contact edge velocity. The expressions we propose are simple analytical functionals of the creep response and should be suitable for experimental data analysis in terms of measured rheologies. We also emphasize the role of the coupling between local stress field at the contact edge and the macroscopic remote loading (far field). We show that the contrast between growing and receding contact originates from the impact of viscoelastic response on coupling, while the separation process at the contact edge is similarly affected by viscoelasticity in both cases.

Etienne Barthel; Christian Frétigny

2009-06-11

296

How acoustic cavitation can improve adhesion.  

PubMed

In general, ultrasound is commonly used at low power level for non-destructive testing (NDT) and detection of delaminations in adhesive bonded structures. The present paper instead presents an approach where power ultrasound is used to improve interface formation prior to the bonding process and to ensure the quality of adhesive bonds by using acoustic cavitation in the liquid adhesive. Results from high-speed videos, rheological and thermal measurements and destructive testing of adhesive bonds with contaminated surfaces are presented and discussed. Power ultrasound can be used in general to improve adhesion and significantly to improve contamination tolerance and robustness of adhesive bonding processes. PMID:22465677

Holtmannspötter, J; Wetzel, M; Czarnecki, J V; Gudladt, H-J

2012-09-01

297

Pine bark extract pycnogenol downregulates IFN-?-induced adhesion of T cells to human keratinocytes by inhibiting inducible ICAM-1 expression  

Microsoft Academic Search

Expression of intercellular adhesion molecule-1 (ICAM-1) is necessary for leukocyte\\/keratinocyte interactions. Upregulation of ICAM-1 expression in keratinocytes has been observed in several inflammatory dermatoses, such as psoriasis, atopic dermatitis, and lupus erythematosus. Inflammatory cytokines, such as interferon-? (IFN-?), upregulate ICAM-1 expression in keratinocytes. Because of potent antioxidant and anti-inflammatory properties of the French maritime pine bark extract, Pycnogenol (Horphag Research,

Toshinori Bito; Sashwati Roy; Chandan K Sen; Lester Packer

2000-01-01

298

Adhesion of Activated Platelets to Endothelial Cells: Evidence for a GPIIbIIIa-dependent Bridging Mechanism and Novel Roles for Endothelial Intercellular Adhesion Molecule 1 (ICAM-1), a v b 3 Integrin, and GPIb a  

Microsoft Academic Search

Summary Although it has been reported that activated platelets can adhere to intact endothelium, the re- ceptors involved have not been fully characterized. Also, it is not clear whether activated plate- lets bind primarily to matrix proteins at sites of endothelial cell denudation or directly to endo- thelial cells. Thus, this study was designed to further clarify the mechanisms of

Thomas Bombeli; Barbara R. Schwartz; John M. Harlan

299

A novel addition polyimide adhesive  

NASA Technical Reports Server (NTRS)

An addition polyimide adhesive, LARC 13, was developed which shows promise for bonding both titanium and composites for applications which require service temperatures in excess of 533 K. The LARC 13 is based on an oligomeric bis nadimide containing a meta linked aromatic diamine. The adhesive melts prior to polymerization due to its oligomeric nature, thereby allowing it to be processed at 344 kPa or less. Therefore, LARC 13 is ideal for the bonding of honeycomb sandwich structures. After melting, the resin thermosets during the cure of the nadic endcaps to a highly crosslinked system. Few volatiles are evolved, thus allowing large enclosed structures to be bonded. Preparation of the adhesive as well as bonding, aging, and testing of lap shear and honeycomb samples are discussed.

St.clair, T. L.; Progar, D. J.

1981-01-01

300

Interfacial adhesion: Theory and experiment  

NASA Technical Reports Server (NTRS)

Adhesion, the binding of different materials at an interface, is of general interest to many branches of technology, e.g., microelectronics, tribology, manufacturing, construction, etc. However, there is a lack of fundamental understanding of such diverse interfaces. In addition, experimental techniques generally have practical objectives, such as the achievement of sufficient strength to sustain mechanical or thermal effects and/or have the proper electronic properties. In addition, the theoretical description of binding at interfaces is quite limited, and a proper data base for such theoretical analysis does not exist. This presentation will review both experimental and theoretical aspects of adhesion in nonpolymer materials. The objective will be to delineate the critical parameters needed, governing adhesion testing along with an outline of testing objectives. A distinction will be made between practical and fundamental objectives. Examples are given where interfacial bonding may govern experimental consideration. The present status of theory is presented along wiith recommendations for future progress and needs.

Ferrante, John; Bozzolo, Guillermo H.; Finley, Clarence W.; Banerjea, Amitava

1988-01-01

301

Adhesive, elastomeric gel impregnating composition  

DOEpatents

An improved capacitor roll with alternating film and foil layers is impregnated with an adhesive, elastomeric gel composition. The gel composition is a blend of a plasticizer, a polyol, a maleic anhydride that reacts with the polyol to form a polyester, and a catalyst for the reaction. The impregnant composition is introduced to the film and foil layers while still in a liquid form and then pressure is applied to aid with impregnation. The impregnant composition is cured to form the adhesive, elastomeric gel. Pressure is maintained during curing.

Shaw, David Glenn (Tucson, AZ); Pollard, John Randolph (Tucson, AZ); Brooks, Robert Aubrey (Tijeras, NM)

2002-01-01

302

Temporal variations of adhesion molecules and matrix metalloproteinases in the course of MS.  

PubMed

Thirty patients with clinically isolated syndromes (CIS) were evaluated at the onset of neurological symptoms and when they developed clinically definite MS (CDMS). Surface expression of LFA-1alpha, VLA-4 and intercellular adhesion molecule-1 (ICAM-1) on PBMC and CSF cells was evaluated using flow cytometry. Serum and CSF concentrations of soluble vascular cell adhesion molecules-1 (VCAM-1), ICAM-1 and E-Selectin, as well as MMP-9 and MMP-2 serum concentrations were assayed using ELISA. Surface expression of LFA-1alpha and VLA-4 molecules on peripheral blood and CSF T cells and monocytes from CIS and CDMS was significantly increased compared with control subjects. Moreover, LFA-1alpha and VLA-4 expression was significantly higher in patients who developed CDMS compared with those with CIS. Similar changes were observed in the serum levels of MMP-9. Furthermore, patients with CIS and CDMS had significantly higher levels of CSF sVCAM and s-E-Selectin than control subjects. These data suggest that VLA-4, LFA-1alpha and MMP-9 play a leading role in the evolution of inflammatory demyelinating lesions in patients with CIS who develop CDMS. PMID:12864990

Correale, Jorge; Bassani Molinas, María de los Milagros

2003-07-01

303

International Journal of Adhesion & Adhesives 25 (2005) 502506 Effects of adhesive type and polystyrene concentration on the shear  

E-print Network

Abstract The lap-shear strengths of adhesively bonded polystyrene (PS), high-density polyethylene (HDPE be effectively bonded with adhesives without expensive surface treatments. r 2005 Elsevier Ltd. All rights an adhesive bond. Typical surface energies of various materials are given in Table 1 [1­3]. Due to the low

304

Controllable adhesion using field-activated fluids  

E-print Network

We demonstrate that field-responsive magnetorheological fluids can be used for variable-strength controllable adhesion. The adhesive performance is measured experimentally in tensile tests (a.k.a. probe-tack experiments) ...

Ewoldt, Randy H.

305

Anisotropic dry adhesive via cap defects.  

PubMed

We demonstrate how introducing a deliberate defect on the overhanging caps of strongly adhering mushroom shaped dry adhesive fibers can produce directional adhesion behavior. We find that the shape and location of this defect controls both the total adhesion force and the degree of directionality for these bio-inspired adhesives. Linear beam theory is used to demonstrate how the application of a shear load to a fiber in tension can create a small compressive load to an asymmetric crack, thereby delaying adhesion failure and producing directional adhesion, and the theory is confirmed with finite element models and empirical data. Anisotropic adhesives have been fabricated and tested and can demonstrate normal adhesion force up to ~250 kPa with a shear displacement of 15 µm away from the defect and as small as ~5 kPa when sheared the same amount towards the defect. PMID:24091647

Khaled, Walid Bin; Sameoto, Dan

2013-12-01

306

Retention with specific and mechanical adhesive lutes.  

PubMed

Attention is drawn to some of the fundamental differences between retention afforded by specifically adhesive and mechanically adhesive lutes. Terms are defined and conclusions drawn regarding the present status and potential of polyacrylate cements. PMID:1057893

Stevens, L

1975-04-01

307

21 CFR 878.4380 - Drape adhesive.  

Code of Federal Regulations, 2013 CFR

... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4380 Drape adhesive. (a) Identification. A drape adhesive is a device...

2013-04-01

308

21 CFR 878.3750 - External prosthesis adhesive.  

Code of Federal Regulations, 2013 CFR

...2013-04-01 false External prosthesis adhesive. 878.3750 Section 878.3750... § 878.3750 External prosthesis adhesive. (a) Identification. An external prosthesis adhesive is a silicone-type adhesive...

2013-04-01

309

21 CFR 878.3750 - External prosthesis adhesive.  

Code of Federal Regulations, 2014 CFR

...2014-04-01 false External prosthesis adhesive. 878.3750 Section 878.3750... § 878.3750 External prosthesis adhesive. (a) Identification. An external prosthesis adhesive is a silicone-type adhesive...

2014-04-01

310

21 CFR 878.3750 - External prosthesis adhesive.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 false External prosthesis adhesive. 878.3750 Section 878.3750... § 878.3750 External prosthesis adhesive. (a) Identification. An external prosthesis adhesive is a silicone-type adhesive...

2010-04-01

311

21 CFR 878.3750 - External prosthesis adhesive.  

Code of Federal Regulations, 2011 CFR

...2011-04-01 false External prosthesis adhesive. 878.3750 Section 878.3750... § 878.3750 External prosthesis adhesive. (a) Identification. An external prosthesis adhesive is a silicone-type adhesive...

2011-04-01

312

A thermally responsive, rigid, and reversible adhesive  

Microsoft Academic Search

In this paper we present the development of a unique self-adhesive material that, unlike conventional adhesives, maintains a high degree of rigidity at the “adhesive” state while possessing the ability to easily de-bond upon heating. Consequently, the material is both a rigid and a reversible adhesive. The material is an initially miscible blend of poly(?-caprolactone) (PCL) and diglycidyl ether of

Xiaofan Luo; Kathryn E. Lauber; Patrick T. Mather

2010-01-01

313

Alterations in cell adhesion proteins and cardiomyopathy  

PubMed Central

Cell adhesive junction is specialized intercellular structure composed of cell adhesion proteins. They are essential to connect adjacent heart muscle cell and make heart contraction effectively and properly. Clinical and genetic studies have revealed close relationship between cell adhesive proteins and the occurrence of various cardiomyopathies. Here we will review recent development on the disease phenotype, potential cellular and molecular mechanism related to cell adhesion molecules, with particular disease pathogenesis learned from genetic manipulated murine models. PMID:24944760

Li, Jifen

2014-01-01

314

A NEW FAMILY OF PRESSURE SENSITIVE ADHESIVES  

Microsoft Academic Search

Adhesive properties of many pressure sensitive or heat-activated adhesives are generally well suited for use with labels on a variety of substrates. However, these adhesives are not significantly solvent-resistant. A solvent-resistant adhesive is highly desirable. Potential applications include tapes and labels for aerospace, automotive, electronics, industrial and consumer articles where exposure to aromatic and aliphatic solvents, oils and fluids occurs.

Roy M. Griswold; Richard Eckberg; Robert Frye

315

Adhesion Recovery and Passive Peeling in a Wall Climbing Robot using Adhesives  

E-print Network

Adhesion Recovery and Passive Peeling in a Wall Climbing Robot using Adhesives Casey Kute, Michael and agile wall climbing robot's ability to regain lost adhesion due to degradation of dry fibrillar. The presented advancements can be applied to other climbing robots using adhesives to allow for safer, more

Wood, Robert

316

Adhesion recovery and passive peeling in a wall climbing robot using adhesives  

Microsoft Academic Search

This paper presents analysis and results for a small and agile wall climbing robot's ability to regain lost adhesion due to degradation of dry fibrillar adhesives. To regain the lost adhesion, two feet are set to the surface and the robot performs a rocking motion on the side where the adhesion has dropped below a safety threshold. The rocking motion

Casey Kute; Michael P. Murphy; Yigit Menguç; Metin Sitti

2010-01-01

317

Adhesion, Deformation, Rolling, and Detachment of a Liquid Capsule on An Adhesive Surface In Shear Flow  

Microsoft Academic Search

3D computational modeling and simulation are presented on adhesion, deformation, rolling and detachment of a liquid capsule on adhesive surfaces in shear flow with an objective to understand the adhesive rolling motion of biological cells, such as leukocyte and cancel cells, and the coupling between cell deformation and biophysics of the adhesive bonds. The computational model is based on an

Vijay Pappu; Prosenjit Bagchi

2008-01-01

318

Parylene to silicon adhesion enhancement  

Microsoft Academic Search

Parylene-C has been used extensively in neural interface devices as a conformal, biocompatible coating; it has also recently become an integrated part of our parylene-cabled silicon probes. However, it is found over the years that its adhesion capability to silicon may be compromised after thermal treatments, cleaning, handling, bench testing and implantations. This paper explores extensively new techniques including annealing,

R. Huang; Y. C. Tai

2009-01-01

319

Photoresist substrate having robust adhesion  

DOEpatents

A substrate material for LIGA applications w hose general composition is Ti/Cu/Ti/SiO.sub.2. The SiO.sub.2 is preferably applied to the Ti/Cu/Ti wafer as a sputtered coating, typically about 100 nm thick. This substrate composition provides improved adhesion for epoxy-based photoresist materials, and particularly the photoresist material SU-8.

Dentinger, Paul M. (Sunol, CA)

2005-07-26

320

New adhesive withstands temperature extremes  

NASA Technical Reports Server (NTRS)

Adhesive, developed for high-temperature components aboard satellites, is useful at both high and low temperatures and exhibits low-vacuum volatility and low shrinkage. System uses polyfunctional epoxy with high aromatic content, low equivalent weight, and more compact polymer than conventional bisphenol A tape.

Park, J. J.; Seidenberg, B.

1978-01-01

321

Switchable Adhesion from Bicomponent Polymeric Brushes  

Microsoft Academic Search

We investigated the adhesive and wetting properties of bicomponent polymeric brushes made from end functionalized hydrophilic and hydrophobic polymer chains. The molecular organization of the mixed brush could be varied reversibly by exposure to selective solvents for the two polymers. Adhesive properties were tested by debonding a flat ended probe from soft pressure-sensitive-adhesives (hydrophobic & hydrophilic) and wetting properties were

Haris Retsos; Ganna Gorodyska; Costantino Creton

2005-01-01

322

Mussel-Inspired Adhesives and Coatings  

Microsoft Academic Search

Mussels attach to solid surfaces in the sea. Their adhesion must be rapid, strong, and tough, or else they will be dislodged and dashed to pieces by the next incoming wave. Given the dearth of synthetic adhesives for wet polar surfaces, much effort has been directed to characterizing and mimicking essential features of the adhesive chemistry practiced by mussels. Studies

Bruce P. Lee; P. B. Messersmith; J. N. Israelachvili; J. H. Waite

2011-01-01

323

Recharging "Hot-Melt" Adhesive Film  

NASA Technical Reports Server (NTRS)

Technique for recharging surface with "hot-melt" film makes use of one sided, high-temperature, pressure-sensitive adhesive tape. Purpose of the one-sided tape is to hold hot-melt charge in place until fused to surface. After adhesive has fused to surface and cooled, tape is removed, leaving adhesive on surface.

Progar, D. J.

1983-01-01

324

Adhesion enhancement of Pd plated leadframes  

Microsoft Academic Search

A surface treatment method for Pd plated leadframes to improve its adhesion to die attach materials and mold compounds is developed. It was found that regardless of the structures of Pd plated leadframes, the adhesion could be increased by up to ten times, compared with untreated Pd plated leadframes. With the improved adhesion, TQFP100 packages with the treated Pd leadframes

C. Q. Cui; H. L. Tay; T. C. Chai

1999-01-01

325

Heparan sulfate proteoglycan on leukemic cells is primarily involved in integrin triggering and its mediated adhesion to endothelial cells  

PubMed Central

Leukocyte migration from circulation into tissue depends on leukocyte integrin-mediated adhesion to endothelium, but integrins cannot function until activated. However, it remains to be understood how tumor cells adhere to endothelium and infiltrate into underlying tissue. We studied mechanisms of extravasation of leukemic cells using adult T cell leukemia (ATL) cells and report the following novel features of cell surface heparan sulfate proteoglycan on ATL cells in ATL cell adhesion to endothelium: ATL cells adhere to endothelial cells through already activated integrins without exogenous stimulation; different from any other hematopoietic cells, ATL cells express a characteristic heparan sulfate capable of immobilizing heparin-binding chemokine macrophage inflammatory protein (MIP)-1 beta, a potent T cell integrin trigger, produced by the cells themselves; competitive interruption of endogenous heparan sulfate proteoglycan synthesis reduces cell surface MIP-1 beta and prevents ATL cells from integrin- mediated adhesion to endothelial cells or intercellular adhesion molecule-1 triggered through G-protein. We propose that leukemic cells adhere to endothelial cells through the adhesion cascade, similar to normal leukocyte, and that the cell surface heparan sulfate, particularly on ATL cells, is pivotally involved in chemokine-dependent autocrine stimulation of integrin triggering by immobilizing the chemokine on them. PMID:8920885

1996-01-01

326

Wet Adhesion and Adhesive Locomotion of Snails on AntiAdhesive Non-Wetting Surfaces  

Microsoft Academic Search

Creating surfaces capable of resisting liquid-mediated adhesion is extremely difficult due to the strong capillary forces that exist between surfaces. Land snails use this to adhere to and traverse across almost any type of solid surface of any orientation (horizontal, vertical or inverted), texture (smooth, rough or granular) or wetting property (hydrophilic or hydrophobic) via a layer of mucus. However,

Neil J. Shirtcliffe; Glen McHale; Michael I. Newton

2012-01-01

327

Viscoelastic analysis of adhesively bonded joints  

NASA Technical Reports Server (NTRS)

An adhesively bonded lap joint is analyzed by assuming that the adherends are elastic and the adhesive is linearly viscoelastic. After formulating the general problem a specific example for two identical adherends bonded through a three parameter viscoelastic solid adhesive is considered. The standard Laplace transform technique is used to solve the problem. The stress distribution in the adhesive layer is calculated for three different external loads, namely, membrane loading, bending, and transverse shear loading. The results indicate that the peak value of the normal stress in the adhesive is not only consistently higher than the corresponding shear stress but also decays slower.

Delale, F.; Erdogan, F.

1980-01-01

328

Nature of the adhesion bond between epoxy adhesive and steel  

NASA Astrophysics Data System (ADS)

The potential difference that appears in the epoxy resin located between two grade 3 steel plates is studied. One of them is stored in epoxy resin to reach equilibrium, and the second plate is coated with an asprepared mixture of epoxy resin with a hardener. It is found that the potential difference decreases in time because of charge transfer by Fe2+ ions through epoxy resin. The luminescence and infrared absorption spectra of the epoxy adhesive on the grade 3 steel surface are recorded. An analysis of these spectra shows that Fe2+ ions penetrate into the as-prepared mixture of epoxy resin with the hardener, and interact with CN groups in the mixture, and form coordination compounds. As a result, a diffusion layer saturated by the coordination compounds forms at the interface between the steel and the adhesive.

Vettegren', V. I.; Mamalimov, R. I.; Savitskii, A. V.; Shcherbakov, I. P.; Sytov, V. V.; Sytov, V. A.

2014-03-01

329

Gecko adhesion pad: a smart surface?  

NASA Astrophysics Data System (ADS)

Recently, it has been shown that humidity can increase the adhesion of the spatula pads that form the outermost (adhesive) surface of the tokay gecko feet by 50% relative to the main adhesion mechanism (i.e. van der Waals adhesive forces), although the mechanism by which the enhancement is realized is still not well understood. A change in the surface hydrophobicity of a gecko setal array is observed when the array, which supports the spatulae, is exposed to a water drop for more than 20 min, suggesting a change in the hydrophilic-lyophilic balance (HLB), and therefore of the conformation of the surface proteins. A surface force apparatus (SFA) was used to quantify these changes, i.e. in the adhesion and friction forces, while shearing the setal array against a silica surface under (i) dry conditions, (ii) 100% humidity and (iii) when fully immersed in water. The adhesion increased in the humid environment but greatly diminished in water. Although the adhesion forces changed significantly, the friction forces remained unaffected, indicating that the friction between these highly textured surfaces is 'load-controlled' rather than 'adhesion-controlled'. These results demonstrate that the gecko adhesive pads have the ability to exploit environmental conditions to maximize their adhesion and stabilize their friction forces. Future designs of synthetic dry adhesives inspired by the gecko can potentially include similar 'smart' surfaces that adapt to their environment.

Pesika, Noshir S.; Zeng, Hongbo; Kristiansen, Kai; Zhao, Boxin; Tian, Yu; Autumn, Kellar; Israelachvili, Jacob

2009-11-01

330

Cell-matrix adhesions in 3D.  

PubMed

Cells in a three-dimensional (3D) extracellular matrix environment often display different properties and behavior compared to cells cultured on a two-dimensional (2D) substrate. Recent studies characterizing the cell-matrix adhesions formed by cells within a 3D matrix have arrived at contradictory conclusions regarding the presence and composition of adhesions. Here we review this literature, and provide a comparative compilation of information found in published studies from the 3D cell-matrix adhesion field in order to identify shared and divergent conclusions and conceptually important areas that require further research. Although there is a general consensus that discrete cell-matrix adhesions exist in various 3D matrix environments, there are specific exceptions, particularly in cells undergoing amoeboid migration. There are also technical issues to consider when imaging adhesions in 3D matrix; for example, over-expression of a cytoskeletal cell adhesion component can potentially cloud the visualization of adhesions and even alter the mode of cell migration. Properties such as stiffness and local matrix topography may also affect the composition of cell-matrix adhesions. For example, even though cells contain integrin-based 3D adhesions, there can be substantial variability within these adhesions in the presence of force-dependent cytoskeletal components such as vinculin. These new findings and ideas provide promising new leads for understanding the regulation and function of cell-matrix adhesions in 3D matrix. PMID:21723391

Harunaga, Jill S; Yamada, Kenneth M

2011-09-01

331

Photochemical tissue bonding with chitosan adhesive films  

PubMed Central

Background Photochemical tissue bonding (PTB) is a promising sutureless technique for tissue repair. PTB is often achieved by applying a solution of rose bengal (RB) between two tissue edges, which are irradiated by a green laser to crosslink collagen fibers with minimal heat production. In this study, RB has been incorporated in chitosan films to create a novel tissue adhesive that is laser-activated. Methods Adhesive films, based on chitosan and containing ~0.1 wt% RB were manufactured and bonded to calf intestine by a solid state laser (? = 532 nm, Fluence~110 J/cm2, spot size~0.5 cm). A single-column tensiometer, interfaced with a personal computer, tested the bonding strength. K-type thermocouples recorded the temperature (T) at the adhesive-tissue interface during laser irradiation. Human fibroblasts were also seeded on the adhesive and cultured for 48 hours to assess cell growth. Results The RB-chitosan adhesive bonded firmly to the intestine with adhesion strength of 15 ± 2 kPa, (n = 31). The adhesion strength dropped to 0.5 ± 0.1 (n = 8) kPa when the laser was not applied to the adhesive. The average temperature of the adhesive increased from 26°C to 32°C during laser exposure. Fibroblasts grew confluent on the adhesive without morphological changes. Conclusion A new biocompatible chitosan adhesive has been developed that bonds photochemically to tissue with minimal temperature increase. PMID:20825632

2010-01-01

332

Theory of adhesion: role of surface roughness  

E-print Network

We discuss how surface roughness influence the adhesion between elastic solids. We introduce a Tabor number which depends on the length scale or magnification, and which gives information about the nature of the adhesion at different length scales. We consider two limiting cases relevant for (a) elastically hard solids with weak adhesive interaction (DMT-limit) and (b) elastically soft solids or strong adhesive interaction (JKR-limit). For the former cases we study the nature of the adhesion using different adhesive force laws ($F\\sim u^{-n}$, $n=1.5-4$, where $u$ is the wall-wall separation). In general, adhesion may switch from DMT-like at short length scales to JKR-like at large (macroscopic) length scale. We compare the theory predictions to the results of exact numerical simulations and find good agreement between theory and the simulation results.

Bo N. J. Persson; Michele Scaraggi

2014-05-13

333

Theory of adhesion: role of surface roughness.  

PubMed

We discuss how surface roughness influences the adhesion between elastic solids. We introduce a Tabor number which depends on the length scale or magnification, and which gives information about the nature of the adhesion at different length scales. We consider two limiting cases relevant for (a) elastically hard solids with weak (or long ranged) adhesive interaction (DMT-limit) and (b) elastically soft solids with strong (or short ranged) adhesive interaction (JKR-limit). For the former cases we study the nature of the adhesion using different adhesive force laws (F ? u(-n), n = 1.5-4, where u is the wall-wall separation). In general, adhesion may switch from DMT-like at short length scales to JKR-like at large (macroscopic) length scale. We compare the theory predictions to results of exact numerical simulations and find good agreement between theory and simulation results. PMID:25273455

Persson, B N J; Scaraggi, M

2014-09-28

334

Theory of adhesion: Role of surface roughness  

NASA Astrophysics Data System (ADS)

We discuss how surface roughness influences the adhesion between elastic solids. We introduce a Tabor number which depends on the length scale or magnification, and which gives information about the nature of the adhesion at different length scales. We consider two limiting cases relevant for (a) elastically hard solids with weak (or long ranged) adhesive interaction (DMT-limit) and (b) elastically soft solids with strong (or short ranged) adhesive interaction (JKR-limit). For the former cases we study the nature of the adhesion using different adhesive force laws (F ˜ u-n, n = 1.5-4, where u is the wall-wall separation). In general, adhesion may switch from DMT-like at short length scales to JKR-like at large (macroscopic) length scale. We compare the theory predictions to results of exact numerical simulations and find good agreement between theory and simulation results.

Persson, B. N. J.; Scaraggi, M.

2014-09-01

335

Ceramic adhesive restorations and biomimetic dentistry: tissue preservation and adhesion.  

PubMed

Thanks to sophisticated adhesive techniques in contemporary dentistry, and the development of composite and ceramic materials, it is possible to reproduce a biomimetic match between substitution materials and natural teeth substrates. Biomimetics or bio-emulation allows for the association of two fundamental parameters at the heart of current therapeutic treatments: tissue preservation and adhesion. This contemporary concept makes the retention of the integrity of the maximum amount of dental tissue possible, while offering exceptional clinical longevity, and maximum esthetic results. It permits the conservation of the biological, esthetic, biomechanical and functional properties of enamel and dentin. Today, it is clearly possible to develop preparations allowing for the conservation of the enamel and dentin in order to bond partial restorations in the anterior and posterior sectors therefore limiting, as Professor Urs Belser from Geneva indicates, "the replacement of previous deficient crowns and devitalized teeth whose conservation are justified but whose residual structural state are insufficient for reliable bonding."1 This article not only addresses ceramic adhesive restoration in the anterior area, the ambassadors of biomimetic dentistry, but also highlights the possibility of occasionally integrating one or two restorations at the heart of the smile as a complement to extensive rehabilitations that require more invasive treatment. PMID:25126616

Tirlet, Gil; Crescenzo, Hélène; Crescenzo, Dider; Bazos, Panaghiotis

2014-01-01

336

STRUCTURAL ORGANIZATION AND FUNCTION OF MOUSE PHOTORECEPTOR RIBBON SYNAPSES INVOLVE THE IMMUNOGLOBULIN ADHESION PROTEIN SYNCAM 1  

PubMed Central

Adhesive interactions in the retina instruct the developmental specification of inner retinal layers. However, potential roles of adhesion in the development and function of photoreceptor synapses remain incompletely understood. This contrasts with our understanding of synapse development in the central nervous system (CNS), which can be guided by select adhesion molecules such as the Synaptic Cell Adhesion Molecule 1 (SynCAM 1/CADM1/Nectin-like 2 protein). This immunoglobulin superfamily protein modulates the development and plasticity of classical excitatory synapses. We now show by immuno-electron microscopy and immunoblotting that SynCAM 1 is expressed on mouse rod photoreceptors and their terminals in the outer nuclear and plexiform layers (ONL and OPL) in a developmentally regulated manner. Expression of SynCAM 1 on rods is low in early postnatal stages (P3-P7), but increases after eye opening (P14). In support of functional roles in the photoreceptors, electroretinogram recordings demonstrate impaired responses to light stimulation in SynCAM 1 knockout (KO) mice. In addition, the structural integrity of synapses in the OPL requires SynCAM 1. Quantitative ultrastructural analysis of SynCAM 1 KO retina measured fewer fully assembled, triadic rod ribbon synapses. Further, rod synapse ribbons are shortened in KO mice and protein levels of Ribeye, a major structural component of ribbons, are reduced in SynCAM 1 KO retina. Together, our results implicate SynCAM 1 in the synaptic organization of the rod visual pathway and provide evidence for novel roles of synaptic adhesion in the structural and functional integrity of ribbon synapses. PMID:23982969

Ribic, Adema; Liu, Xinran; Crair, Michael C.; Biederer, Thomas

2013-01-01

337

Adhesion formation to hemostatic agents and its reduction with a sodium hyaluronate/carboxymethylcellulose adhesion barrier.  

PubMed

The impact of hemostatic agents on postoperative adhesion formation has not been well studied. We hypothesized that hemostatic agents would be a significant nidus for adhesion formation and that a resorbable barrier would effectively reduce adhesions to hemostatic agents. Four commercial hemostatic agents, each composed of a different biomaterial matrix, were implanted in female Sprague-Dawley rats, and adhesion formation was examined 7 days after surgery. In separate studies, the effects of serosal trauma (via cecal abrasion), added blood, and the presence of chemically modified sodium hyaluronate/carboxymethylcellulose (HA/CMC) barrier on adhesion formation to hemostatic agents were studied. Significant adhesions formed to hemostatic agents even in the absence of traumatized tissue. When applied after cecal abrasion, the incidence of adhesions to the hemostatic agents increased. Addition of blood to this model increased adhesion formation even further, causing adhesions in every animal in the study. An HA/CMC adhesion barrier reduced adhesions to hemostatic agents in the presence of serosal trauma and maintained effectiveness even in the presence of blood. In conclusion, hemostatic agents potentiated adhesion formation at the site of application in a model without trauma. In more challenging models, their adhesiogenic contribution was overwhelmed by trauma and blood. HA/CMC adhesion barrier applied over hemostatic agents at the time of surgery provided significant protection against postoperative adhesions in these preclinical models. PMID:22488970

Greenawalt, Keith E; Corazzini, Rubina L; Colt, M Jude; Holmdahl, Lena

2012-07-01

338

Tissue Adhesives as Active Implants  

Microsoft Academic Search

\\u000a Tissue adhesives are substances that hold tissues together, and could be broadly applicable in medicine and surgery. In appropriate\\u000a circumstances, such materials could be attractive alternatives to sutures and staples since they can be applied more quickly,\\u000a causes less pain and may require less equipment. In addition, there is no risk to the practitioner from sharp instruments\\u000a (Singer et al.,

Boaz Mizrahi; Christopher Weldon; Daniel S. Kohane

339

Clinical considerations for adhesive bridgework.  

PubMed

Many dental practitioners do not use adhesive bridges because of concerns over high failure rates. Techniques for these restorations should be based on the fundamental principles of bridge design which require rigid, accurately fitting frameworks and careful control of the occlusion. The abutments generally require little if any tooth preparation. Greater security will result from more extensive coverage of abutment teeth: the routine use of relative axial tooth movement is a predictable method for creating the space that this approach requires. PMID:15242258

Ibbetson, Richard

2004-06-01

340

76 FR 59087 - Approval and Promulgation of Air Quality Implementation Plans; Delaware; Adhesives and Sealants Rule  

Federal Register 2010, 2011, 2012, 2013, 2014

...commercially-used adhesive and sealant products...4.0--Adhesives and Sealants under Regulation...and use of adhesives, sealants, adhesive primers, or sealant primers...application of adhesives, sealants, adhesive primers, and sealant primers...

2011-09-23

341

Chitosan Adhesive Films for Photochemical Tissue Bonding  

NASA Astrophysics Data System (ADS)

Photochemical tissue bonding (PTB) is a promising sutureless technique for tissue repair. PTB is often achieved by applying a solution of rose bengal (RB) between two tissue edges, which are irradiated by a green laser to crosslink collagen fibers with minimal heat production. In this study, RB has been incorporated in chitosan films to create a novel tissue adhesive that is laser-activated. Materials and Methods. Adhesive films, based on chitosan and containing ˜0.1wt% RB were manufactured and bonded to calf intestine by a solid state laser (wavelength = 532 nm, Fluence ˜110 J/cm2, spot size ˜5 mm). A single-column tensiometer, interfaced with a personal computer, tested the bonding strength. K-type thermocouples recorded the temperature (T) at the adhesive-tissue interface during laser irradiation. Human fibroblasts were also seeded on the adhesive and cultured for 48 hours to assess cell growth. Results and Conclusion. The RB-chitosan adhesive bonded firmly to the intestine (15±2 kPa, n = 31). The adhesion strength dropped to 0.5±0.1 kPa (n = 8) when the laser was not applied to the adhesive. The average temperature of the adhesive increased from 26 °C to 32 °C during laser exposure. Fibroblasts grew confluent on the adhesive without morphological changes. A new biocompatible chitosan adhesive has been developed that bonds photochemically to tissue with minimal temperature increase.

Lauto, Antonio; Mawad, Damia; Barton, Matthew; Piller, Sabine C.; Longo, Leonardo

2011-08-01

342

Human Neutrophil Flow Chamber Adhesion Assay  

PubMed Central

Neutrophil firm adhesion to endothelial cells plays a critical role in inflammation in both health and disease. The process of neutrophil firm adhesion involves many different adhesion molecules including members of the ?2 integrin family and their counter-receptors of the ICAM family. Recently, naturally occurring genetic variants in both ?2 integrins and ICAMs are reported to be associated with autoimmune disease. Thus, the quantitative adhesive capacity of neutrophils from individuals with varying allelic forms of these adhesion molecules is important to study in relation to mechanisms underlying development of autoimmunity. Adhesion studies in flow chamber systems can create an environment with fluid shear stress similar to that observed in the blood vessel environment in vivo. Here, we present a method using a flow chamber assay system to study the quantitative adhesive properties of human peripheral blood neutrophils to human umbilical vein endothelial cell (HUVEC) and to purified ligand substrates. With this method, the neutrophil adhesive capacities from donors with different allelic variants in adhesion receptors can be assessed and compared. This method can also be modified to assess adhesion of other primary cell types or cell lines. PMID:25045887

Zhou, Yebin; Kucik, Dennis F.; Szalai, Alexander J.; Edberg, Jeffrey C.

2014-01-01

343

Different effects of Porphyromonas gingivalis lipopolysaccharide and TLR2 agonist Pam3CSK4 on the adhesion molecules expression in endothelial cells.  

PubMed

Recent researches suggest an association between periodontitis and cardiovascular disease. Periodontopathic bacteria and/or their component might play a role in the development of atherosclerotic lesions. In the present study, we investigated in vitro the effect of Porphyromonas gingivalis lipopolysaccharide (LPS) on the expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs) as well as its effect on Escherichia coli LPS-induced response. The effect of P. gingivalis LPS was compared with that of toll-like receptor 2 agonist synthetic triacylated lipoprotein Pam3-Cys-Ser-(Lys)4 (Pam3CSK4). Gene and protein expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin were measured using RT-PCR and flow cytometry, respectively. P. gingivalis LPS stimulated the expression levels of all adhesion molecules in a dose-dependent manner. However, the response of HUVECs to P. gingivalis LPS was markedly lower than that to E. coli LPS. Moreover, P. gingivalis LPS attenuated E. coli LPS-induced responses when HUVECs were simultaneously stimulated with both kinds of LPS. Treatment with Pam3CSK4 resulted in a minor increase of adhesion molecule expression and did not diminish E. coli LPS-induced responses. Our data suggest that P. gingivalis LPS induces in vitro the expression of adhesion molecules in endothelial cells, which might promote atherogenesis. Qualitatively different responses of HUVECs to P. gingivalis LPS and Pam3CSK4 suggest that besides TLR2 other signaling pathways might be involved in the effects of P. gingivalis LPS. PMID:24375077

Andrukhov, Oleh; Steiner, Ilse; Liu, Shutai; Bantleon, Hans Peter; Moritz, Andreas; Rausch-Fan, Xiaohui

2015-01-01

344

Role of scavenger receptor class B type I and sphingosine 1-phosphate receptors in high density lipoprotein-induced inhibition of adhesion molecule expression in endothelial cells.  

PubMed

We characterized the molecular mechanisms by which high density lipoprotein (HDL) inhibits the expression of adhesion molecules, including vascular cell adhesion molecule-1 and intercellular adhesion molecule-1, induced by sphingosine 1-phosphate (S1P) and tumor necrosis factor (TNF) alpha in endothelial cells. HDL inhibited S1P-induced nuclear factor kappaB activation and adhesion molecule expression in human umbilical vein endothelial cells. The inhibitory HDL actions were associated with nitric-oxide synthase (NOS) activation and were reversed by inhibitors for phosphatidylinositol 3-kinase and NOS. The HDL-induced inhibitory actions were also attenuated by the down-regulation of scavenger receptor class B type I (SR-BI) and its associated protein PDZK1. When TNFalpha was used as a stimulant, the HDL-induced NOS activation and the inhibitory action on adhesion molecule expression were, in part, attenuated by the down-regulation of the expression of S1P receptors, especially S1P(1), in addition to SR-BI. Reconstituted HDL composed mainly of apolipoprotein A-I and phosphatidylcholine mimicked the SR-BI-sensitive part of HDL-induced actions. Down-regulation of S1P(3) receptors severely suppressed the stimulatory actions of S1P. Although G(i/o) proteins may play roles in either stimulatory or inhibitory S1P actions, as judged from pertussis toxin sensitivity, the coupling of S1P(3) receptors to G(12/13) proteins may be critical to distinguish the stimulatory pathways from the inhibitory ones. In conclusion, even though S1P alone stimulates adhesion molecule expression, HDL overcomes S1P(3) receptor-mediated stimulatory actions through SR-BI/PDZK1-mediated signaling pathways involving phosphatidylinositol 3-kinase and NOS. In addition, the S1P component of HDL plays a role in the inhibition of TNFalpha-induced actions through S1P receptors, especially S1P(1). PMID:17046831

Kimura, Takao; Tomura, Hideaki; Mogi, Chihiro; Kuwabara, Atsushi; Damirin, Alatangaole; Ishizuka, Tamotsu; Sekiguchi, Akihiro; Ishiwara, Mitsuteru; Im, Doon-Soon; Sato, Koichi; Murakami, Masami; Okajima, Fumikazu

2006-12-01

345

Pharmacological activation of PPAR gamma ameliorates vascular endothelial insulin resistance via a non-canonical PPAR gamma-dependent nuclear factor-kappa B trans-repression pathway.  

PubMed

Vascular endothelial insulin resistance (IR) is a critically initial factor in cardiocerebrovascular events resulted from diabetes and is becoming a worldwide public health issue. Thiazolidinediones (TZDs) are clinical insulin-sensitizers acting through a canonical peroxisome proliferator-activated receptor gamma (PPAR?)-dependent insulin trans-activation pathway. However, it remains elusive whether there are other mechanisms. In current study, we investigated whether TZDs improve endothelial IR induced by high glucose concentration or hyperglycemia via a non-canonical PPAR?-dependent nuclear factor-kappa B (NF-?B) trans-repression pathway. Our results showed that pre-treatment with TZDs dramatically decrease the susceptibility of endothelial cell to IR, while post-treatment notably improve the endothelial IR both in vitro and in vivo. Moreover, TZDs substantially increase the levels of endothelial nitric oxide synthase (eNOS) and inhibitory ?B alpha (I?B?), whereas decrease those of the phosphorylated inhibitory ?B kinase alpha/beta (phosphor-IKK?/?) and the cytokines including tumor necrosis factor alpha (TNF?), interleukin-6 (IL-6), soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cellular adhesion molecule-1 (sVCAM-1), suggesting that TZDs act indeed through a PPAR?-dependent NF-?B trans-repression pathway. These findings highlighted a non-canonical mechanism for TZDs to ameliorate endothelial IR which might provide a potential strategy to prevent and treat the diabetic vascular complications clinically. PMID:25687252

Zhang, Ying; Zhan, Ri-Xin; Chen, Jun-Qun; Gao, Yan; Chen, Li; Kong, Ying; Zhong, Xiao-Juan; Liu, Mei-Qi; Chu, Jia-Jia; Yan, Guo-Qiang; Li, Teng; He, Ming; Huang, Qi-Ren

2015-05-01

346

NR-150B2 adhesive development  

NASA Technical Reports Server (NTRS)

Adhesive based polyimide solutions which are more easily processed than conventional aromatic polyimide systems and show potential for use for extended times at 589K are discussed. The adhesive system is based on a solution containing diglyme as the solvent and 2,2 bis(3',4'-dicarboxyphenyl)hexafluoropropane, paraphenylenediamine, and oxydianiline. The replacement of N-methylpyrrolidone with diglyme as the solvent was found to improve the adhesive strengths of lap shear samples and simplify the processing conditions for bonding both titanium and graphite fiber/polyimide matrix resin composites. Information was obtained on the effects of various environments including high humidity, immersion in jet fuel and methylethylketone on aluminum filled adhesive bonds. The adhesive was also evaluated in wide area bonds and flatwise tensile specimens using titanium honeycomb and composite face sheets. It was indicated that the developed adhesive system has the potential for use in applications requiring long term exposure to at least 589K (600 F).

Blatz, P. S.

1978-01-01

347

Adhesion and wear resistance of materials  

NASA Technical Reports Server (NTRS)

Recent studies into the nature of bonding at the interface between two solids in contact or a solid and deposited film have provided a better understanding of those properties important to the adhesive wear resistance of materials. Analytical and experimental progress are reviewed. For simple metal systems the adhesive bond forces are related to electronic wave function overlap. With metals in contact with nonmetals, molecular-orbital energy, and density of states, respectively can provide insight into adhesion and wear. Experimental results are presented which correlate adhesive forces measured between solids and the electronic surface structures. Orientation, surface reconstruction, surface segregation, adsorption are all shown to influence adhesive interfacial strength. The interrelationship between adhesion and the wear of the various materials as well as the life of coatings applied to substrates are discussed. Metallic systems addressed include simple metals and alloys and these materials in contact with themselves, both oxide and nonoxide ceramics, diamond, polymers, and inorganic coating compounds, h as diamondlike carbon.

Buckley, D. H.

1986-01-01

348

Mussel-Inspired Adhesives and Coatings  

PubMed Central

Mussels attach to solid surfaces in the sea. Their adhesion must be rapid, strong, and tough, or else they will be dislodged and dashed to pieces by the next incoming wave. Given the dearth of synthetic adhesives for wet polar surfaces, much effort has been directed to characterizing and mimicking essential features of the adhesive chemistry practiced by mussels. Studies of these organisms have uncovered important adaptive strategies that help to circumvent the high dielectric and solvation properties of water that typically frustrate adhesion. In a chemical vein, the adhesive proteins of mussels are heavily decorated with Dopa, a catecholic functionality. Various synthetic polymers have been functionalized with catechols to provide diverse adhesive, sealant, coating, and anchoring properties, particularly for critical biomedical applications. PMID:22058660

Lee, Bruce P.; Messersmith, P.B.; Israelachvili, J.N.; Waite, J.H.

2011-01-01

349

VISCOELASTIC AND FATIGUE PROPERTIES OF DENTAL ADHESIVES AND THEIR IMPACT ON DENTIN-ADHESIVE INTERFACE DURABILITY  

E-print Network

The clinical performance of composite tooth restoration under cyclic loading is impacted by the behavior of the dentin-adhesive (d-a) interface, which is a complex construct of different material components. Dentin adhesive is a significant material...

Singh, Viraj

2009-12-22

350

Adhesions following cesarean delivery: a review of their occurrence, consequences and preventative management using adhesion barriers.  

PubMed

The objective of this article is to provide a comprehensive review of the occurrence and consequences of postoperative adhesions following cesarean delivery (CD), and an overview of the published clinical data on prevention in this setting using adhesion barriers. Adhesions occur frequently after CD and the incidence increases with each subsequent CD. Repeat CDs are complicated by adhesions, which increase operating time, time to delivery and risk of bladder injury. Clinical data on the efficacy of adhesion prevention strategies specific to the setting of CD are limited. Two small, nonrandomized studies found that the use of absorbable anti-adhesion barriers was associated with a significant reduction in adhesion formation and a shorter time to delivery at repeat CD, compared with no barrier use. Implications for practice and research are discussed. There is a significant need for well-controlled, randomized clinical studies investigating adhesion prevention in the labor and delivery setting. PMID:24007252

Poole, Judith H

2013-09-01

351

Improved Cure-in-Place Silicone Adhesives  

NASA Technical Reports Server (NTRS)

Two improved cure-in-place silicone-elastomer-based adhesives have low thermal expansion and low thermal conductivity. Adhesives are flexible at low temperature and withstand high temperatures without disintegrating. New ablative compounds were initially developed for in-flight repair of insulating tile on Space Shuttle orbiter. Could find use in other applications requiring high-performance adhesives, such as sealants for solar collectors.

Blevins, C. E.; Sweet, J.; Gonzalez, R.

1982-01-01

352

Wet Performance of Biomimetic Fibrillar Adhesives  

Microsoft Academic Search

\\u000a A number of legged organisms have evolved sophisticated, fibrillar attachment schemes that exhibit functional qualities highly\\u000a desirable in synthetic reversible adhesives: substrate compliance, high adhesive strength, and sustained performance over\\u000a many attach\\/release cycles (Creton and Gorb, 2007; Peattie, 2008). While a number of early synthetic mimics of fibrillar adhesives as well as the biological systems that inspired\\u000a them are effective

K. H. Aaron Lau; Phillip B. Messersmith

353

Bone Adhesives in Trauma and Orthopedic Surgery  

Microsoft Academic Search

Adhesives, especially bone adhesives, are resorbed and degraded to non-toxic products after fulfilling their function in contact\\u000a with the living organism. The use of such bone adhesives has found growing interest in all fields of medicine in the last\\u000a 50 years. The dream of trauma and orthopedic surgeons for alternatives to osteosynthesis and pins is reflected in the development\\u000a of

Christian Heiss; Ralf Kraus; Dominique Schluckebier; Ann-Christin Stiller; Sabine Wenisch; Reinhard Schnettler

2006-01-01

354

Compound Charpy specimens by adhesive joining  

NASA Astrophysics Data System (ADS)

Compound (reconstituted) Charpy specimens were manufactured by an adhesive joining method in which each half of a previously tested specimen formed the central section of a new testpiece. 29 adhesives were screened to select the most suitable. Compound specimens were precracked and used as minature fracture mechanics specimens and tested in both 3-point static bending and impact. The results are in good agreement with those of conventional specimens. Recommendations for the most appropriate commercial adhesive for hot cell operations are given.

Ghoneim, M. M.; Hammad, F. H.; Pachur, D.; Britz, L.

1992-03-01

355

Protein adhesion of block copolymer surfaces  

Microsoft Academic Search

Atomic force microscopy studies on the adhesion between various novel poly(methyl methacrylate)\\/poly(acrylic acid)-based block\\u000a copolymers and the proteins fibronectin and bovine serum albumin are presented for the first time. Random, diblock, and triblock\\u000a copolymers exhibit distinct adhesion profiles although their chemical compositions are identical, implying that biomaterial\\u000a nanomorphology can be used to control protein–polymer interactions and potentially cell adhesion.

Scott R. Schricker; Manuel L. B. Palacio; Bharat Bhushan

2011-01-01

356

Chlorinated polyolefins as adhesion promoters for plastics  

SciTech Connect

A new procedure which tests the peel strength of a topcoat applied to a substrate was used to quantitatively determine the effectiveness of a chlorinated pololefin as an adhesion promoter for coatings on plastics. A 4 to 5-fold increase in adhesion resulted for a latex vinyl acrylic topcoat that was applied to polypropylene. Electron Spectroscopy for Chemical Analysis studies showed a topcoat/chlorinated polyolefin adhesive failure for both melamine crosslinked and urethane crosslinked polyester systems on polypropylene. Topcoat/chlorinated polyolefin adhesive failure also occured for both melamine crosslinked and urethane crosslinked polyester systems on thermal plastic olefins. All failure tests were performed at ambient conditions.

Lawniczak, J.; Sass, C.; Stoffer, J.O.; Dechent, W.L. [Univ. of Missouri, Rolla, MO (United States)

1993-12-31

357

Effect of water absorption on pollen adhesion.  

PubMed

Pollens possess a thin liquid coating, pollenkitt, which plays a major role in adhesion by forming capillary menisci at interfaces. Unfortunately, the influence of humidity on pollenkitt properties and capillary adhesion is unknown. Because humidity varies widely in the environment, the answers have important implications for better understanding plant reproduction, allergy and asthma, and pollen as atmospheric condensation nuclei. Here, pollenkitt-mediated adhesion of sunflower pollen to hydrophilic and hydrophobic surfaces was measured as a function of humidity. The results quantify for the first time the significant water absorption of pollenkitt and the resulting complex dependence of adhesion on humidity. On hydrophilic Si, adhesion increased with increasing RH for pollens with or without pollenkitt, up to 200nN at 70% RH. In contrast, on hydrophobic PS, adhesion of pollenkitt-free pollen is independent of RH. Surprisingly, when pollenkitt was present adhesion forces on hydrophobic PS first increased with RH up to a maximum value at 35% RH (?160nN), and then decreased with further increases in RH. Independent measurement of pollenkitt properties is used with models of capillary adhesion to show that humidity-dependent changes in pollenkitt wetting and viscosity are responsible for this complex adhesion behavior. PMID:25524008

Lin, Haisheng; Lizarraga, Leonardo; Bottomley, Lawrence A; Carson Meredith, J

2015-03-15

358

Anisotropic adhesion of micropillars with spatula pads.  

PubMed

Natural gecko adhesive structures consisting of angled setae, branched into thin spatulas, have remarkable properties including easily attachable and releasable anisotropic adhesion. The geometrically asymmetric structures lead to anisotropic adhesive properties. Inspired by the gecko, we fabricated an array of micropillars with asymmetric spatula pads from elastomeric materials. This paper describes the anisotropic properties of the micropillars with spatula pads as established by experimental measurements and observation together with finite element analysis. The results indicate that the structural difference of the spatula pad at one edge of the micropillar provides the anisotropic adhesive properties. PMID:24446878

Seo, Seungwan; Lee, Jehong; Kim, Kwang-Seop; Ko, Kwang Hee; Lee, Jong Hyun; Lee, Jongho

2014-02-12

359

Nucleation and growth of cadherin adhesions  

SciTech Connect

Cell-cell contact formation relies on the recruitment of cadherin molecules and their anchoring to actin. However, the precise chronology of events from initial cadherin trans-interactions to adhesion strengthening is unclear, in part due to the lack of access to the distribution of cadherins within adhesion zones. Using N-cadherin expressing cells interacting with N-cadherin coated surfaces, we characterized the formation of cadherin adhesions at the ventral cell surface. TIRF and RIC microscopies revealed streak-like accumulations of cadherin along actin fibers. FRAP analysis indicated that engaged cadherins display a slow turnover at equilibrium, compatible with a continuous addition and removal of cadherin molecules within the adhesive contact. Association of cadherin cytoplasmic tail to actin as well as actin cables and myosin II activity are required for the formation and maintenance of cadherin adhesions. Using time lapse microscopy we deciphered how cadherin adhesions form and grow. As lamellipodia protrude, cadherin foci stochastically formed a few microns away from the cell margin. Neo-formed foci coalesced aligned and coalesced with preformed foci either by rearward sliding or gap filling to form cadherin adhesions. Foci experienced collapse at the rear of cadherin adhesions. Based on these results, we present a model for the nucleation, directional growth and shrinkage of cadherin adhesions.

Lambert, Mireille [INSERM, U839, Paris, F-75005 (France); Universite Pierre et Marie Curie-Paris6, Paris, Institut du Fer a Moulin, UMR-S0839, Paris, F-75005 (France); Thoumine, Olivier [Universite Bordeaux 2, CNRS, UMR5091, Institut Francois Magendie de Neurosciences, Bordeaux, F-33077 (France); Brevier, Julien [Universite Joseph Fourier, CNRS, UMR5588, Saint-Martin d'Heres, F-38402 (France); Choquet, Daniel [Universite Bordeaux 2, CNRS, UMR5091, Institut Francois Magendie de Neurosciences, Bordeaux, F-33077 (France); Riveline, Daniel [Universite Joseph Fourier, CNRS, UMR5588, Saint-Martin d'Heres, F-38402 (France); Mege, Rene-Marc [INSERM, U839, Paris, F-75005 (France); Universite Pierre et Marie Curie-Paris6, Paris, Institut du Fer a Moulin, UMR-S0839, Paris, F-75005 (France)], E-mail: mege@fer-a-moulin.inserm.fr

2007-11-15

360

Investigation of package sealing using organic adhesives  

NASA Technical Reports Server (NTRS)

A systematic study was performed to evaluate the suitability of adhesives for sealing hybrid packages. Selected adhesives were screened on the basis of their ability to seal gold-plated Kovar butterfly-type packages that retain their seal integrity after individual exposures to increasingly severe temperature-humidity environments. Tests were also run using thermal shock, temperature cycling, mechanical shock and temperature aging. The four best adhesives were determined and further tested in a 60 C/98% RH environment and continuously monitored in regard to moisture content. Results are given, however, none of the tested adhesives passed all the tests.

Perkins, K. L.; Licari, J. J.

1977-01-01

361

The development of aerospace polyimide adhesives  

NASA Technical Reports Server (NTRS)

Few materials are available which can be used as aerospace adhesives at temperatures in the range of 300 C. The Materials Division at NASA-Langley Research Center developed several high temperature polyimide adhesives to fulfill the stringent needs of current aerospace programs. These adhesives are the result of a decade of basic research studies on the structure property relationships of both linear and addition aromatic polyimides. The development of both in house and commercially available polyimides is reviewed with regards to their potential for use as aerospace adhesives.

St.clair, A. K.; St.clair, T. L.

1983-01-01

362

Adhesion as a weapon in microbial competition.  

PubMed

Microbes attach to surfaces and form dense communities known as biofilms, which are central to how microbes live and influence humans. The key defining feature of biofilms is adhesion, whereby cells attach to one another and to surfaces, via attachment factors and extracellular polymers. While adhesion is known to be important for the initial stages of biofilm formation, its function within biofilm communities has not been studied. Here we utilise an individual-based model of microbial groups to study the evolution of adhesion. While adhering to a surface can enable cells to remain in a biofilm, consideration of within-biofilm competition reveals a potential cost to adhesion: immobility. Highly adhesive cells that are resistant to movement face being buried and starved at the base of the biofilm. However, we find that when growth occurs at the base of a biofilm, adhesion allows cells to capture substratum territory and force less adhesive, competing cells out of the system. This process may be particularly important when cells grow on a host epithelial surface. We test the predictions of our model using the enteric pathogen Vibrio cholerae, which produces an extracellular matrix important for biofilm formation. Flow cell experiments indicate that matrix-secreting cells are highly adhesive and form expanding clusters that remove non-secreting cells from the population, as predicted by our simulations. Our study shows how simple physical properties, such as adhesion, can be critical to understanding evolution and competition within microbial communities. PMID:25290505

Schluter, Jonas; Nadell, Carey D; Bassler, Bonnie L; Foster, Kevin R

2015-01-01

363

Comparison of three work of adhesion measurements  

SciTech Connect

Practical work of adhesion measurements are being studied for several types of polymer/metal combinations in order to obtain a better understanding of the adhesive failure mechanisms for systems containing encapsulated and bonded components. The primary question is whether studies of model systems can be extended to systems of technological interest. The authors report on their first attempts to obtain the work of adhesion between a PDMS polymer and stainless steel. The work of adhesion measurements were made using three techniques -- contact angle, adhesive fracture energy at low deformation rates and JKR. Previous work by Whitesides` group show a good correlation between JKR and contact angle measurements for PDMS. Their initial work focused on duplicating the PDMS measurements of Chaudury. In addition, in this paper the authors extend the work of adhesion measurement to third technique -- interfacial failure energy. The ability to determine the reversible work of adhesion for practical adhesive joints allows understanding of several issues that control adhesion: surface preparation, nature of the interphase region, and bond durability.

Emerson, J.A.; O`Toole, E.; Zamora, D. [Sandia National Labs., Albuquerque, NM (United States). Dept. of Organic Materials; Poon, B. [Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Macromolecular Science

1998-02-01

364

[Regulation of adhesion molecules by calcium].  

PubMed

Adhesion molecules play a major role in many biological and pathological processes and are essential to the development and construction of tissue architecture. Cadherins are a family of membrance receptors that mediate calcium-dependent homophilic cell-cell adhesion. Secretins is also known to require calcium to function as an adhesion molecule, whereas integrin needs cation including calcium to stabilize its alpha-subunit to bind to its counterpart. Of interest, it was reported that intracellular calcium regulates cellular functions by affecting the expression of adhesion molecule in plasma membrane. PMID:15775341

Inaba, Masaaki

2002-05-01

365

Classification of OPP adhesive tapes according to pyrogram of adhesives.  

PubMed

Pressure sensitive adhesives (PSAs) of colorless and transparent oriented polypropylene (OPP) adhesive tapes were analyzed by pyrolysis/gas chromatography/mass spectrometry (Py/GC/MS). The PSAs were acrylic and rubber-based PSAs and the tapes were classified according to total ion current (TIC) chromatograms of the PSAs. The main pyrolyzates of the acrylic PSAs were decomposition products of monomers, monomers, dimmers and trimers. Those of the rubber-based PSAs were the monomers of elastomers, and subtle peaks observed were the pyrolyzates of tackifiers and volatile additives in the TIC chromatograms. Small differences were observed among the classifications of the acrylic PSAs by Py/GC/MS, attenuated total reflection Fourier transform infrared (ATR FT-IR) and Matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The classification of the rubber-based PSAs by Py/GC/MS and that by ATR FT-IR were the same, and a slight difference was observed between those by Py/GC/MS and MALDI MS. PMID:20719441

Kumooka, Y

2011-03-20

366

Adhesive for solar control film  

SciTech Connect

A water-activatable adhesive useful for adhering a solar film, polyester (polyethylene terephthalate) film, to glass or to metal substrates. The adhesive comprises the reacted product of (A) gamma-isocyanatopropyltriethoxy silane, containing a free isocyanate (NCO) group, and (B) a thermoplastic polyester formed by reacting (i) a dibasic acid selected from the group consisting of terephthalic acid, isophthalic acid and hexahydrophthalic acid, and mixtures thereof, with (ii) a polymethylene glycol of the formula HO(CH/sub 2/) /SUB x/ OH where x is an integer from 2 to 10, neopentyl glycol and glycerin, and mixtures thereof, and (iii) an aliphatic dibasic acid selected from the group consisting of those having the formula HOOC(CH/sub 2/) /SUB n/ COOH where n is an integer from 1 to 8, and mixtures of such acids, whereby substantially no free NCO remains in the adhesive. Solar film is used for absorbing and/or reflecting solar radiation. Solar film can be a single sheet of polyester dyed sufficiently to absorb the glare of bright sunlight, or it can be a single sheet of polyester, on one side of which a reflective metal (most often aluminum) is deposited in an amount which can be totally reflective or in an amount which still allows visible light transmission and over which a protective coating is deposited, or it can be a laminated structure of the reflective film adhered to a clear or dyed polyester film by which means the reflective metal is sandwiched between two layers of polyester film, or it can be a laminated structure of a reflective film to a polyolefin film.

Penn, H.J.

1984-01-31

367

Bacterial Adhesion at Synthetic Surfaces  

PubMed Central

A systematic investigation into the effect of surface chemistry on bacterial adhesion was carried out. In particular, a number of physicochemical factors important in defining the surface at the molecular level were assessed for their effect on the adhesion of Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli. The primary experiments involved the grafting of groups varying in hydrophilicity, hydrophobicity, chain length, and chemical functionality onto glass substrates such that the surfaces were homogeneous and densely packed with functional groups. All of the surfaces were found to be chemically well defined, and their measured surface energies varied from 15 to 41 mJ · m?2. Protein adsorption experiments were performed with 3H-labelled bovine serum albumin and cytochrome c prior to bacterial attachment studies. Hydrophilic uncharged surfaces showed the greatest resistance to protein adsorption; however, our studies also showed that the effectiveness of poly(ethyleneoxide) (PEO) polymers was not simply a result of its hydrophilicity and molecular weight alone. The adsorption of the two proteins approximately correlated with short-term cell adhesion, and bacterial attachment for L. monocytogenes and E. coli also correlated with the chemistry of the underlying substrate. However, for S. aureus and S. typhimurium a different pattern of attachment occurred, suggesting a dissimilar mechanism of cell attachment, although high-molecular-weight PEO was still the least-cell-adsorbing surface. The implications of this for in vivo attachment of cells suggest that hydrophilic passivating groups may be the best method for preventing cell adsorption to synthetic substrates provided they can be grafted uniformly and in sufficient density at the surface. PMID:10543814

Cunliffe, D.; Smart, C. A.; Alexander, C.; Vulfson, E. N.

1999-01-01

368

Electrical detection of kidney injury molecule-1 with AlGaN/GaN high electron mobility transistors  

SciTech Connect

AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect kidney injury molecule-1 (KIM-1), an important biomarker for early kidney injury detection. The gate region consisted of 5 nm gold deposited onto the AlGaN surface. The gold was conjugated to highly specific KIM-1 antibodies through a self-assembled monolayer of thioglycolic acid. The HEMT source-drain current showed a clear dependence on the KIM-1 concentration in phosphate-buffered saline solution. The limit of detection was 1 ng/ml using a 20x50 {mu}m{sup 2} gate sensing area. This approach shows potential for both preclinical and clinical kidney injury diagnosis with accurate, rapid, noninvasive, and high throughput capabilities.

Wang, H. T.; Kang, B. S.; Ren, F.; Pearton, S. J.; Johnson, J. W.; Rajagopal, P.; Roberts, J. C.; Piner, E. L.; Linthicum, K. J. [Department of Chemical Engineering, University of Florida, Gainesville, Florida 32611 (United States); Department of Materials Science and Engineering, University of Florida, Gainesville, Florida 32611 (United States); Nitronex Corporation, Durham, North Carolina 27703 (United States)

2007-11-26

369

Tissue adhesive using synthetic model adhesive proteins inspired by the marine mussel  

Microsoft Academic Search

The surface free energy and its dispersion and polar components of pigskin were determined by wettability measurements. The contact angles and work of adhesion of solutions of the synthetic model adhesive sequence poly(Gly-Tyr-Lys) inspired by marine adhesive proteins were measured on the epidermis and the dermis of pigskin. Also the surface free energy of pigskin was determined using contact angles

H. Tatehata; A. Mochizuki; K. Ohkawa; M. Yamada; H. Yamamoto

2001-01-01

370

Cell Adhesion Strength Is Controlled by Intermolecular Spacing of Adhesion Receptors  

E-print Network

Cell Adhesion Strength Is Controlled by Intermolecular Spacing of Adhesion Receptors C. Selhuber numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force forces as well as the stiffness of the cell body were significantly decreased compared to spacings %50 nm

Schwarz, Ulrich

371

Effect of Inorganic Fillers in Paper on the Adhesion of Pressure-Sensitive Adhesives  

Microsoft Academic Search

Inorganic fillers are inexpensive materials used to increase the density, smoothness and other properties of paper that are important for printing. In the current study, the adhesion of pressure-sensitive adhesives (PSAs), a common type of adhesive used in labels and tapes, to papers containing varying amounts and types of fillers is investigated. Papers with three types of fillers, precipitated calcium

Weixu Chen; Xiaoyan Tang; John Considine; Kevin T. Turner

2011-01-01

372

Characterization of Adhesion in Pressure Sensitive Adhesives with a Spherical Indenter  

Microsoft Academic Search

A combination of interfacial effects and bulk viscoelastic behavior controls the performance of pressure sensitive adhesives. We have studied these issues using commercially available adhesive transfer tapes. Using a spherical indenter, these experiments measure the displacement of the indenter into the adhesive as a function of the applied normal contact force. With the assumption of linear elastic behavior under small

Alfred Crosby; Kenneth R. Shull

1997-01-01

373

Effects of an energy-restricted diet rich in plant-derived ?-linolenic acid on systemic inflammation and endothelial function in overweight-to-obese patients with metabolic syndrome traits.  

PubMed

Plant-derived ?-linolenic acid (ALA) may reduce the risk of CVD, possibly by decreasing systemic inflammation and improving endothelial function. In the present study, the effects of a hypoenergetic diet rich in ALA (3·4 g/d) on the biomarkers of systemic inflammation and vascular function were investigated in eighty-one overweight-to-obese patients with metabolic syndrome traits in comparison with a hypoenergetic diet low in ALA (0·9 g/d, control). After a 6-month dietary intervention, there were significant decreases in the serum concentrations of C-reactive protein (CRP), TNF-?, IL-6, soluble intercellular adhesion molecule-1 (sICAM-1), soluble endothelial selectin (sE-selectin) and asymmetric dimethylarginine in both dietary groups. However, no inter-group differences were observed for all these changes. The serum concentration of YKL-40 (human cartilage glycoprotein 39 or chitinase-3-like protein 1) decreased after the ALA diet when compared with the control diet (P< 0·05 for time × treatment interaction). Plasma concentrations of fibrinogen did not significantly change in the two dietary groups. The decreases in the serum concentrations of sICAM-1, sE-selectin, CRP and YKL-40 were significantly correlated with the decreases in body fat mass. In conclusion, the present study indicates that in overweight-to-obese patients with metabolic syndrome traits, both vascular function and inflammation are improved during body-weight loss. The high ALA intake led to a more pronounced reduction in the serum concentration of YKL-40 compared with the intake of the low-ALA control diet, indicating the existence of independent favourable physiological effects of ALA during weight loss. PMID:25180479

Egert, Sarah; Baxheinrich, Andrea; Lee-Barkey, Young Hee; Tschoepe, Diethelm; Wahrburg, Ursel; Stratmann, Bernd

2014-10-28

374

Association of obesity and biomarkers of inflammation and endothelial dysfunction in adults in Inner Mongolia, China  

PubMed Central

Background Recent studies suggest that central obesity is an important predictor of cardiovascular disease (CVD) in addition to overall obesity. Both inflammation and endothelial dysfunction are associated with increased risk of CVD. We examined the association between body mass index (BMI) and waist circumference (WC) with plasma concentrations of biomarkers of inflammation and endothelial dysfunction. Methods We conducted a cross-sectional study of 2589 lean, moderately active participants aged 20 years and older in Inner Mongolia, China. Overnight fasting blood samples were obtained to measure the biomarkers including C-reactive protein (CRP), soluble inter-cellular adhesion molecule-1 (sICAM-1), soluble E-selectin (sE-selectin), and angiotensin II. Height, body weight, and WC were measured by trained staff and BMI was calculated (kg/m2). Results In univariate analysis, CRP, sICAM-1, and sE-selectin were all significantly higher among individuals with a higher BMI and WC. In multivariate analysis, each standard deviation (SD) increase in WC (9.6 cm) was associated with about 46% higher risk (odds ratio [OR] 1.46, 95% confidence interval [CI] 1.21–1.76) of elevated CRP but a 1 SD increase in BMI (3.5 kg/m2) was not associated with the risk of elevated CRP (OR 0.96, 95% CI 0.80–1.16). However, each SD increase in BMI was associated with about 30% higher risk of having elevated E-selectin (OR 1.30, 95% CI 1.08–1.55). Conclusions WC is a stronger predictor of inflammation while BMI is a stronger predictor for endothelial dysfunction. These results suggest measuring both BMI and WC will help to assess the risk of CVD in the Chinese population. PMID:20439121

Thompson, Angela M.; Zhang, Yonghong; Tong, Weijun; Xu, Tan; Chen, Jing; Zhao, Li; Kelly, Tanika N.; Chen, Chung-Shiuan; Bazzano, Lydia A.; He, Jiang

2015-01-01

375

Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers  

PubMed Central

Background It is estimated that over 8 million people in the United Kingdom suffer from osteoarthritis. These patients may require orthopaedic surgical intervention to help alleviate their clinical condition. Investigations presented here was to test the hypothesis that total hip replacement (THR) and total knee replacement (TKR) orthopaedic surgery result in changes to leukocyte and endothelial markers thus increasing inflammatory reactions postoperatively. Methods During this 'pilot study', ten test subjects were all scheduled for THR or TKR elective surgery due to osteoarthritis. Leukocyte concentrations were measured using an automated full blood count analyser. Leukocyte CD11b (Mac-1) and CD62L cell surface expression, intracellular production of H2O2 and elastase were measured as markers of leukocyte function. Von Willebrand factor (vWF) and soluble intercellular adhesion molecule-1 (sICAM-1) were measured as markers of endothelial activation. Results The results obtained during this study demonstrate that THR and TKR orthopaedic surgery result in similar changes of leukocyte and endothelial markers, suggestive of increased inflammatory reactions postoperatively. Specifically, THR and TKR surgery resulted in a leukocytosis, this being demonstrated by an increase in the total leukocyte concentration following surgery. Evidence of leukocyte activation was demonstrated by a decrease in CD62L expression and an increase in CD11b expression by neutrophils and monocytes respectively. An increase in the intracellular H2O2 production by neutrophils and monocytes and in the leukocyte elastase concentrations was also evident of leukocyte activation following orthopaedic surgery. With respect to endothelial activation, increases in vWF and sICAM-1 concentrations were demonstrated following surgery. Conclusion In general it appeared that most of the leukocyte and endothelial markers measured during these studies peaked between days 1-3 postoperatively. It is proposed that by allowing orthopaedic surgeons access to alternative laboratory markers such as CD11b, H2O2 and elastase, CD62L, vWF and sICAM-1, an accurate assessment of the extent of inflammation due to surgery per se could be made. Ultimately, the leukocyte and endothelial markers assessed during this investigation may have a role in monitoring potential infectious complications that can occur during the postoperative period. PMID:20148137

2010-01-01

376

Synovial phenotypes in rheumatoid arthritis correlate with response to biologic therapeutics  

PubMed Central

Introduction Rheumatoid arthritis (RA) is a complex and clinically heterogeneous autoimmune disease. Currently, the relationship between pathogenic molecular drivers of disease in RA and therapeutic response is poorly understood. Methods We analyzed synovial tissue samples from two RA cohorts of 49 and 20 patients using a combination of global gene expression, histologic and cellular analyses, and analysis of gene expression data from two further publicly available RA cohorts. To identify candidate serum biomarkers that correspond to differential synovial biology and clinical response to targeted therapies, we performed pre-treatment biomarker analysis compared with therapeutic outcome at week 24 in serum samples from 198 patients from the ADACTA (ADalimumab ACTemrA) phase 4 trial of tocilizumab (anti-IL-6R) monotherapy versus adalimumab (anti-TNF?) monotherapy. Results We documented evidence for four major phenotypes of RA synovium – lymphoid, myeloid, low inflammatory, and fibroid - each with distinct underlying gene expression signatures. We observed that baseline synovial myeloid, but not lymphoid, gene signature expression was higher in patients with good compared with poor European league against rheumatism (EULAR) clinical response to anti-TNF? therapy at week 16 (P =0.011). We observed that high baseline serum soluble intercellular adhesion molecule 1 (sICAM1), associated with the myeloid phenotype, and high serum C-X-C motif chemokine 13 (CXCL13), associated with the lymphoid phenotype, had differential relationships with clinical response to anti-TNF? compared with anti-IL6R treatment. sICAM1-high/CXCL13-low patients showed the highest week 24 American College of Rheumatology (ACR) 50 response rate to anti-TNF? treatment as compared with sICAM1-low/CXCL13-high patients (42% versus 13%, respectively, P =0.05) while anti-IL-6R patients showed the opposite relationship with these biomarker subgroups (ACR50 20% versus 69%, P =0.004). Conclusions These data demonstrate that underlying molecular and cellular heterogeneity in RA impacts clinical outcome to therapies targeting different biological pathways, with patients with the myeloid phenotype exhibiting the most robust response to anti-TNF?. These data suggest a path to identify and validate serum biomarkers that predict response to targeted therapies in rheumatoid arthritis and possibly other autoimmune diseases. Trial registration ClinicalTrials.gov NCT01119859 PMID:25167216

2014-01-01

377

21 CFR 864.6650 - Platelet adhesion test.  

Code of Federal Regulations, 2012 CFR

...2012-04-01 2012-04-01 false Platelet adhesion test. 864.6650 Section...Manual Hematology Devices § 864.6650 Platelet adhesion test. (a) Identification. A platelet adhesion test is a device used to...

2012-04-01

378

21 CFR 864.6650 - Platelet adhesion test.  

Code of Federal Regulations, 2014 CFR

...2014-04-01 2014-04-01 false Platelet adhesion test. 864.6650 Section...Manual Hematology Devices § 864.6650 Platelet adhesion test. (a) Identification. A platelet adhesion test is a device used to...

2014-04-01

379

21 CFR 864.6650 - Platelet adhesion test.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Platelet adhesion test. 864.6650 Section...Manual Hematology Devices § 864.6650 Platelet adhesion test. (a) Identification. A platelet adhesion test is a device used to...

2010-04-01

380

21 CFR 864.6650 - Platelet adhesion test.  

Code of Federal Regulations, 2013 CFR

...2013-04-01 2013-04-01 false Platelet adhesion test. 864.6650 Section...Manual Hematology Devices § 864.6650 Platelet adhesion test. (a) Identification. A platelet adhesion test is a device used to...

2013-04-01

381

7 CFR 2902.16 - Adhesive and mastic removers.  

Code of Federal Regulations, 2010 CFR

...2010-01-01 2010-01-01 false Adhesive and mastic removers. 2902...Designated Items § 2902.16 Adhesive and mastic removers. (a...and tile mastics as well as adhesive materials, including glue, tape, and gum, from...

2010-01-01

382

49 CFR 587.16 - Adhesive bonding procedure.  

Code of Federal Regulations, 2010 CFR

... 2010-10-01 false Adhesive bonding procedure. 587.16 Section...Deformable Barrier § 587.16 Adhesive bonding procedure. Immediately before...these can adversely affect bonding. The adhesive is applied to one...

2010-10-01

383

49 CFR 587.16 - Adhesive bonding procedure.  

Code of Federal Regulations, 2012 CFR

... 2012-10-01 false Adhesive bonding procedure. 587.16 Section...Deformable Barrier § 587.16 Adhesive bonding procedure. Immediately before...these can adversely affect bonding. The adhesive is applied to one...

2012-10-01

384

7 CFR 3201.16 - Adhesive and mastic removers.  

Code of Federal Regulations, 2013 CFR

...2013-01-01 2013-01-01 false Adhesive and mastic removers. 3201...Designated Items § 3201.16 Adhesive and mastic removers. (a...and tile mastics as well as adhesive materials, including glue, tape, and gum, from...

2013-01-01

385

49 CFR 587.16 - Adhesive bonding procedure.  

Code of Federal Regulations, 2011 CFR

... 2011-10-01 false Adhesive bonding procedure. 587.16 Section...Deformable Barrier § 587.16 Adhesive bonding procedure. Immediately before...these can adversely affect bonding. The adhesive is applied to one...

2011-10-01

386

7 CFR 2902.16 - Adhesive and mastic removers.  

Code of Federal Regulations, 2011 CFR

...2011-01-01 2011-01-01 false Adhesive and mastic removers. 2902...Designated Items § 2902.16 Adhesive and mastic removers. (a...and tile mastics as well as adhesive materials, including glue, tape, and gum, from...

2011-01-01

387

49 CFR 587.16 - Adhesive bonding procedure.  

Code of Federal Regulations, 2014 CFR

... 2014-10-01 false Adhesive bonding procedure. 587.16 Section...Deformable Barrier § 587.16 Adhesive bonding procedure. Immediately before...these can adversely affect bonding. The adhesive is applied to one...

2014-10-01

388

INTERPENETRATING POLYMER NETWORK (IPN) ADHESIVES FOR ELECTRON BEAM CURE  

EPA Science Inventory

Electron beam (e-beam)-processed polymer adhesives have historically performed poorly compared to traditional adhesive technologies due to a lack of toughness engineered into these new types of adhesive materials. Consequently, sequential- and simultaneous-interpenetrating polyme...

389

7 CFR 3201.16 - Adhesive and mastic removers.  

Code of Federal Regulations, 2014 CFR

...2014-01-01 2014-01-01 false Adhesive and mastic removers. 3201...Designated Items § 3201.16 Adhesive and mastic removers. (a...and tile mastics as well as adhesive materials, including glue, tape, and gum, from...

2014-01-01

390

7 CFR 3201.16 - Adhesive and mastic removers.  

Code of Federal Regulations, 2012 CFR

...2012-01-01 2012-01-01 false Adhesive and mastic removers. 3201...Designated Items § 3201.16 Adhesive and mastic removers. (a...and tile mastics as well as adhesive materials, including glue, tape, and gum, from...

2012-01-01

391

49 CFR 587.16 - Adhesive bonding procedure.  

Code of Federal Regulations, 2013 CFR

... 2013-10-01 false Adhesive bonding procedure. 587.16 Section...Deformable Barrier § 587.16 Adhesive bonding procedure. Immediately before...these can adversely affect bonding. The adhesive is applied to one...

2013-10-01

392

Adhesions: What Are They and How Can They Be Prevented?  

MedlinePLUS

... would be helpful to you. Complications of adhesions Infertility and pregnancy complications. Adhesions that form on a woman's ovaries or fallopian tubes make infertility more likely. Women with adhesions in or around ...

393

Peritoneal Adhesions: Etiology, Pathophysiology, and Clinical Significance  

Microsoft Academic Search

Aim: To summarize the most common etiologic factors and describe the pathophysiology in the formation of peritoneal adhesions, to outline their clinical significance and consequences, and to evaluate the pharmacologic, mechanical, and surgical adjuvant strategies to minimize peritoneal adhesion formation. Methods: We performed an extensive MEDLINE search of the internationally published English literature of all medical and epidemiological journal articles,

Theodoros Liakakos; Nikolaos Thomakos; Paul M. Fine; Christos Dervenis; Ronald L. Young

2001-01-01

394

Wood Structure and Adhesive Bond Strength  

Microsoft Academic Search

Much of the literature on the bonding of wood and other lignocellulosic materials has concentrated on traditional adhesion theories. This has led to misconceptions because wood is a porous material on both the macroscopic and microscopic levels. A better understanding of wood bonding can be developed by investigating the theories of adhesion and bond strength, taking into consideration the unusual

Charles R. Frihart

395

Glass-ionomer cements as adhesives  

Microsoft Academic Search

The literature on the clinical use of glass-ionomer cements is reviewed, and this shows that these materials are successful partly because of the good adhesion they exhibit towards a variety of substrates encountered in dentistry. The reasons for this good adhesion are identified as the good initial wetting of the surfaces met in clinical dentistry, the development of strong chemical

A. O. Akinmade; J. W. Nicholson

1993-01-01

396

Hot-Melt Adhesive Attachment System  

NASA Technical Reports Server (NTRS)

Adhesive system is as effective on Earth as in space. Fiberglass cloth mounted in head assembly. When adhesive reaches melt temperature head is attached to metals composites, ceramics, and other materials. Once attached, head cooled rapidly for quick stick. Used to tether tools or attach temporary scaffolding to walls, buildings, or beams.

Fox, R. L.; Frizzell, A. W.; Little, B. D.; Progar, D. J.; Coultrip, R. H.; Couch, R. H.; Stein, B. A.; Buckley, J. D.; St. Clair, T. L.; Gleason, J. R.

1983-01-01

397

Fracture load predictions for adhesive joints  

Microsoft Academic Search

An engineering approach to fracture load predictions for adhesive joints is presented. The approach is based on the premise that the in-situ strength of the bondline can be characterized by the fracture envelope (critical energy release rate as a function of the mode of loading), for a specific adhesive system. By using the J integral for large deformations together with

G. Fernlund; M. Papini; D. McCammond; J. K. Spelt

1994-01-01

398

ISOLATION OF INTEGRIN-BASED ADHESION COMPLEXES  

PubMed Central

The integration of cells with their extracellular environment is facilitated by cell surface adhesion receptors, such as integrins, which play important roles in both normal development and the onset of pathologies. Engagement of integrins with their ligands in the extracellular matrix, or counter receptors on other cells, initiates the intracellular assembly of a wide variety of proteins into adhesion complexes such as focal contacts, focal adhesions and fibrillar adhesions. The proteins recruited to these complexes mediate bidirectional signalling across the plasma membrane and as such help to coordinate and / or modulate the multitude of physical or chemical signals to which the cell is subjected. The protocols in this unit describe two approaches for the isolation or enrichment of proteins contained within integrin-associated adhesion complexes together with their local plasma membrane / cytosolic environments from cells in culture. In the first protocol integrin-associated adhesion structures are affinity isolated using microbeads coated with extracellular ligands or antibodies. The second protocol describes the isolation of ventral membrane preparations that are enriched for adhesion complex structures. The protocols permit the determination of adhesion complex components by subsequent downstream analysis by Western blotting or mass spectrometry. PMID:25727331

Jones, Matthew C.; Humphries, Jonathan D.; Byron, Adam; Millon-Frémillon, Angelique; Robertson, Joseph; Paul, Nikki R.; Ng, Daniel H. J.; Askari, Janet A.; Humphries, Martin J.

2015-01-01

399

Anisotropic conducting adhesives for electronic assembly  

Microsoft Academic Search

Presents some experimental and theoretical results from research exploring the design rules and relevant process parameters in the assembly of electronic components using anisotropic conductive adhesive materials. The experimental configurations studies have geometries representative of flip-chip and micro ball grid array chip scale packaging. Evaluates a range of materials combinations, including (random filled) adhesive materials based on both thermoplastic and

David C. Whalley; Samjid H. Mannan; David J. Williams

1997-01-01

400

Biochemical investigations of retinotectal adhesive specificity  

Microsoft Academic Search

The preferential adhesion of chick neural retina cells to surfaces of intact optic tecta has been investigated biochemically. The study uses a collection assay in which single cells from either dorsal or ventral halves of neural retina adhere preferentially to ventral or dorsal halves of optic tecta, respectively. The data presented support the following conclusions: (a) The adhesion of ventral

RICHARD B. MARCHASE

1977-01-01

401

Surgical glues: are they really adhesive?  

Microsoft Academic Search

Objective: The aim of this study is to create a standard test to approve the efficacy of a surgical sealant. An industrial test, the bulge-and-blister test, which is very convenient for measuring adhesion energy, is applied to the surgical field to quantify adhesion of bioadhesives. Methods: Samples were composed of two circular layers of equine pericardium glued by the surgical

Bertrand R. M. Perrin; Michel Dupeux; Piergiorgio Tozzi; Dominique Delay; Philippe Gersbach; Ludwig K. von Segesser

2009-01-01

402

Adhesive Loose Packings of Small Particles  

E-print Network

We explore adhesive loose packings of dry small spherical particles of micrometer size using 3D discrete-element simulations with adhesive contact mechanics. A dimensionless adhesion parameter ($Ad$) successfully combines the effects of particle velocities, sizes and the work of adhesion, identifying a universal regime of adhesive packings for $Ad>1$. The structural properties of the packings in this regime are well described by an ensemble approach based on a coarse-grained volume function that includes correlations between bulk and contact spheres. Our theoretical and numerical results predict: (i) An equation of state for adhesive loose packings that appears as a continuation from the frictionless random close packing (RCP) point in the jamming phase diagram; (ii) The existence of a maximal loose packing point at the coordination number $Z=2$ and packing fraction $\\phi=1/2^{3}$. Our results highlight that adhesion leads to a universal packing regime at packing fractions much smaller than the random loose packing, which can be described within a statistical mechanical framework. We present a general phase diagram of jammed matter comprising frictionless, frictional, adhesive as well as non-spherical particles, providing a classification of packings in terms of their continuation from the spherical frictionless RCP.

Wenwei Liu; Shuiqing Li; Adrian Baule; Hernán A. Makse

2014-10-08

403

Reversibility and mechanism of bacterial adhesion  

Microsoft Academic Search

The reversibility and mechanisms of adhesion of various pseudomonads and coryneform bacteria having different hydrophobicities and negative cell surface charges on negatively charged Teflon and glass were studied. Adhesion at an ionic strength of 0.1 M was irreversible and corresponded to activation Gibbs energies for detachment higher than 5 kT for 19 out of 20 combinations of bacterial strains and

Huub H. M. Rijnaarts; Willem Norde; Edward J. Bouwer; Johannes Lyklema; Alexander J. B. Zehnder

1995-01-01

404

Electrospinning protein nanofibers to control cell adhesion  

Microsoft Academic Search

The structural and mechanical properties of a surface often play an integral part in the determination of the cell adhesion strength and design parameters for creating a biodegradable electrospun scaffold. Nanofibers composed of the globular proteins bovine serum albumin (BSA) and fibronectin were produced by electrospinning with the electrospun protein scaffold serving as an extracellular matrix to which adhesion interaction

Cynthia Chinwe Nwachukwu

2010-01-01

405

Tensile and shear strength of adhesives  

NASA Technical Reports Server (NTRS)

This experiment is conducted in a freshman-level course: Introduction to Engineering Materials. There are no prerequisites for the course although students should have some knowledge of basic algebra. The objectives are to tension and shear test adhesives and to determine the tensile and shear properties of adhesives. Details of equipment of procedure are given.

Stibolt, Kenneth A.

1990-01-01

406

Enzymatically Degradable Mussel-Inspired Adhesive Hydrogel  

PubMed Central

Mussel-inspired adhesive hydrogels represent innovative candidate medical sealants or glues. In the present work, we describe an enzyme-degradable mussel-inspired adhesive hydrogel formulation, achieved by incorporating minimal elastase substrate peptide Ala-Ala into the branched poly(ethylene glycol) (PEG) macromonomer structure. The system takes advantage of neutrophil elastase expression upregulation and secretion from neutrophils upon recruitment to wounded or inflamed tissue. By integrating adhesive degradation behaviors that respond to cellular cues, we expand the functional range of our mussel-inspired adhesive hydrogel platforms. Rapid (<1 min) and simultaneous gelation and adhesion of the proteolytically active, catechol-terminated precursor macromonomer was achieved by addition of sodium periodate oxidant. Rheological analysis and equilibrium swelling studies demonstrated that the hydrogel is appropriate for soft tissue-contacting applications. Notably, hydrogel storage modulus (G?) achieved values on the order of 10 kPa, and strain at failure exceeded 200% strain. Lap shear testing confirmed the material’s adhesive behavior (shear strength: 30.4 ± 3.39 kPa). Although adhesive hydrogel degradation was not observed during short-term (27 h) in vitro treatment with neutrophil elastase, in vivo degradation proceeded over several months following dorsal subcutaneous implantation in mice. This work represents the first example of an enzymatically degradable mussel-inspired adhesive and expands the potential biomedical applications of this family of materials. PMID:22059927

2011-01-01

407

Marine mussel adhesion: biochemistry, mechanisms, and biomimetics  

Microsoft Academic Search

Common blue mussel (Mytilus edulis) is a sessile organism that has unique ability to attach to a wide array of organic and inorganic marine surfaces using its holdfast structures. Strong adhesion to surfaces is essential for mussel survival, movement, and self-defense. Mussel proteins from byssal thread are structural components connecting soft mussel tissues to marine surfaces via an adhesive plaque

Nandika Bandara; Hongbo Zeng; Jianping Wu

2012-01-01

408

A batch fabricated biomimetic dry adhesive  

Microsoft Academic Search

The fine hair adhesive system found in nature is capable of reversibly adhering to just about any surface. This dry adhesive, best demonstrated in the pad of the gecko, makes use of a multilevel conformal structure to greatly increase inelastic surface contact, enhancing short range interactions and producing significant amounts of attractive forces. Recent work has attempted to reproduce and

Michael T Northen; Kimberly L Turner

2005-01-01

409

Selective Adhesion of Thiobacillus ferrooxidans to Pyrite  

PubMed Central

Bacterial adhesion to mineral surfaces plays an important role not only in bacterial survival in natural ecosystems, but also in mining industry applications. Selective adhesion was investigated with Thiobacillus ferrooxidans by using four minerals, pyrite, quartz, chalcopyrite, and galena. Escherichia coli was used as a control bacterium. Contact angles were used as indicators of hydrophobicity, which was an important factor in the interaction between minerals and bacteria. The contact angle of E. coli in a 0.5% sodium chloride solution was 31°, and the contact angle of T. ferrooxidans in a pH 2.0 sulfuric acid solution was 23°. E. coli tended to adhere to more hydrophobic minerals by hydrophobic interaction, while T. ferrooxidans selectively adhered to iron-containing minerals, such as pyrite and chalcopyrite. Ferrous ion inhibited the selective adhesion of T. ferrooxidans to pyrite competitively, while ferric ion scarcely inhibited such adhesion. When selective adhesion was quenched by ferrous ion completely, adhesion of T. ferrooxidans was controlled by hydrophilic interactions. Adhesion of E. coli to pyrite exhibited a liner relationship on langmuir isotherm plots, but adhesion of T. ferrooxidans did not. T. ferrooxidans recognized the reduced iron in minerals and selectively adhered to pyrite and chalcopyrite by a strong interaction other than the physical interaction. PMID:16349106

Ohmura, Naoya; Kitamura, Keiko; Saiki, Hiroshi

1993-01-01

410

Isolation of integrin-based adhesion complexes.  

PubMed

The integration of cells with their extracellular environment is facilitated by cell surface adhesion receptors, such as integrins, which play important roles in both normal development and the onset of pathologies. Engagement of integrins with their ligands in the extracellular matrix, or counter-receptors on other cells, initiates the intracellular assembly of a wide variety of proteins into adhesion complexes such as focal contacts, focal adhesions, and fibrillar adhesions. The proteins recruited to these complexes mediate bidirectional signaling across the plasma membrane, and, as such, help to coordinate and/or modulate the multitude of physical and chemical signals to which the cell is subjected. The protocols in this unit describe two approaches for the isolation or enrichment of proteins contained within integrin-associated adhesion complexes, together with their local plasma membrane/cytosolic environments, from cells in culture. In the first protocol, integrin-associated adhesion structures are affinity isolated using microbeads coated with extracellular ligands or antibodies. The second protocol describes the isolation of ventral membrane preparations that are enriched for adhesion complex structures. The protocols permit the determination of adhesion complex components via subsequent downstream analysis by western blotting or mass spectrometry. © 2015 by John Wiley & Sons, Inc. PMID:25727331

Jones, Matthew C; Humphries, Jonathan D; Byron, Adam; Millon-Frémillon, Angélique; Robertson, Joseph; Paul, Nikki R; Ng, Daniel H J; Askari, Janet A; Humphries, Martin J

2015-01-01

411

Nondestructive testing of adhesively-bonded joints  

Microsoft Academic Search

One approach to testing the suitability of an adhesive joint for a particular application is to build and test to destruction a representative sample of the joint. In this way the best adhesive and surface treatment for a given application can be found. To reduce the costs of this approach, the designer will wish to call on previous experience with

R. D. Adams; B. W. Drinkwater

1997-01-01

412

Adhesion, friction and micromechanical properties of ceramics  

NASA Technical Reports Server (NTRS)

The adhesion, friction, and micromechanical properties of ceramics, both in monolithic and coating form, are reviewed. Ceramics are examined in contact with themselves, other harder materials, and metals. For the simplicity of discussion, the tribological properties of concern in the processes are separated into two parts. The first part discusses the pull-off force (adhesion) and the shear force required to break the interfacial junctions between contacting surfaces. The role of chemical bonding in adhesion and friction, and the effects of surface contaminant films and temperature on tribological response with respect to adhesion and friction are discussed. The second part deals with abrasion of ceramics. Elastic, plastic, and fracture behavior of ceramics in solid state contact is discussed. The scratch technique of determining the critical load needed to fracture interfacial adhesive bonds of ceramic deposited on substrates is also addressed.

Miyoshi, Kazuhisa

1988-01-01

413

Effects of nitrogen plasma treatment of pressure-sensitive adhesive layer surfaces on their peel adhesion behavior  

Microsoft Academic Search

The influence of the surface modification of pressure-sensitive adhesive tapes on their adhesion behavior has been investigated. PBA [poly(butyl acrylate)] and PIB [poly(isobutylene)] adhesives were chosen as pressure-sensitive adhesives and nitrogen plasma was used for the surface modification of the adhesives. The peel force of PBA or PIB adhesive\\/stainless steel joints was evaluated. The nitrogen plasma treatment showed large effects

M. Kawabe; S. Tasaka; N. Inagaki

1999-01-01

414

MRI of placental adhesive disorder.  

PubMed

Placental adhesive disorder (PAD) is a serious pregnancy complication that occurs when the chorionic villi invade the myometrium. Placenta praevia and prior caesarean section are the two important risk factors. PAD is classified on the basis of the depth of myometrial invasion (placenta accreta, placenta increta and placenta percreta). MRI is the preferred image modality for pre-natal diagnosis of PAD and as complementary technique when ultrasonography is inconclusive. Imaging findings that are helpful for the diagnosis include dark intraplacental bands, direct invasion of adjacent structures by placental tissue, interruption of normal trilayered myometrium and uterine bulging. Clinicians should be aware of imaging features of PAD to facilitate optimal patient management. PMID:25060799

Srisajjakul, S; Prapaisilp, P; Bangchokdee, S

2014-10-01

415

Peritoneal adhesions after laparoscopic gastrointestinal surgery  

PubMed Central

Although laparoscopy has the potential to reduce peritoneal trauma and post-operative peritoneal adhesion formation, only one randomized controlled trial and a few comparative retrospective clinical studies have addressed this issue. Laparoscopy reduces de novo adhesion formation but has no efficacy in reducing adhesion reformation after adhesiolysis. Moreover, several studies have suggested that the reduction of de novo post-operative adhesions does not seem to have a significant clinical impact. Experimental data in animal models have suggested that CO2 pneumoperitoneum can cause acute peritoneal inflammation during laparoscopy depending on the insufflation pressure and the surgery duration. Broad peritoneal cavity protection by the insufflation of a low-temperature humidified gas mixture of CO2, N2O and O2 seems to represent the best approach for reducing peritoneal inflammation due to pneumoperitoneum. However, these experimental data have not had a significant impact on the modification of laparoscopic instrumentation. In contrast, surgeons should train themselves to perform laparoscopy quickly, and they should complete their learning curves before testing chemical anti-adhesive agents and anti-adhesion barriers. Chemical anti-adhesive agents have the potential to exert broad peritoneal cavity protection against adhesion formation, but when these agents are used alone, the concentrations needed to prevent adhesions are too high and could cause major post-operative side effects. Anti-adhesion barriers have been used mainly in open surgery, but some clinical data from laparoscopic surgeries are already available. Sprays, gels, and fluid barriers are easier to apply in laparoscopic surgery than solid barriers. Results have been encouraging with solid barriers, spray barriers, and gel barriers, but they have been ambiguous with fluid barriers. Moreover, when barriers have been used alone, the maximum protection against adhesion formation has been no greater than 60%. A recent small, randomized clinical trial suggested that the combination of broad peritoneal cavity protection with local application of a barrier could be almost 100% effective in preventing post-operative adhesion formation. Future studies should confirm the efficacy of this global strategy in preventing adhesion formation after laparoscopy by focusing on clinical end points, such as reduced incidences of bowel obstruction and abdominal pain and increased fertility. PMID:24803803

Mais, Valerio

2014-01-01

416

Improving controllable adhesion on both rough and smooth surfaces with a hybrid electrostatic/gecko-like adhesive.  

PubMed

This paper describes a novel, controllable adhesive that combines the benefits of electrostatic adhesives with gecko-like directional dry adhesives. When working in combination, the two technologies create a positive feedback cycle whose adhesion, depending on the surface type, is often greater than the sum of its parts. The directional dry adhesive brings the electrostatic adhesive closer to the surface, increasing its effect. Similarly, the electrostatic adhesion helps engage more of the directional dry adhesive fibrillar structures, particularly on rough surfaces. This paper presents the new hybrid adhesive's manufacturing process and compares its performance to three other adhesive technologies manufactured using a similar process: reinforced PDMS, electrostatic and directional dry adhesion. Tests were performed on a set of ceramic tiles with varying roughness to quantify its effect on shear adhesive force. The relative effectiveness of the hybrid adhesive increases as the surface roughness is increased. Experimental data are also presented for different substrate materials to demonstrate the enhanced performance achieved with the hybrid adhesive. Results show that the hybrid adhesive provides up to 5.1× greater adhesion than the electrostatic adhesive or directional dry adhesive technologies alone. PMID:24451392

Ruffatto, Donald; Parness, Aaron; Spenko, Matthew

2014-04-01

417

Improving controllable adhesion on both rough and smooth surfaces with a hybrid electrostatic/gecko-like adhesive  

PubMed Central

This paper describes a novel, controllable adhesive that combines the benefits of electrostatic adhesives with gecko-like directional dry adhesives. When working in combination, the two technologies create a positive feedback cycle whose adhesion, depending on the surface type, is often greater than the sum of its parts. The directional dry adhesive brings the electrostatic adhesive closer to the surface, increasing its effect. Similarly, the electrostatic adhesion helps engage more of the directional dry adhesive fibrillar structures, particularly on rough surfaces. This paper presents the new hybrid adhesive's manufacturing process and compares its performance to three other adhesive technologies manufactured using a similar process: reinforced PDMS, electrostatic and directional dry adhesion. Tests were performed on a set of ceramic tiles with varying roughness to quantify its effect on shear adhesive force. The relative effectiveness of the hybrid adhesive increases as the surface roughness is increased. Experimental data are also presented for different substrate materials to demonstrate the enhanced performance achieved with the hybrid adhesive. Results show that the hybrid adhesive provides up to 5.1× greater adhesion than the electrostatic adhesive or directional dry adhesive technologies alone. PMID:24451392

Ruffatto, Donald; Parness, Aaron; Spenko, Matthew

2014-01-01

418

Distribution of cell adhesion molecules on CD56++, CD3-, CD16- large granular lymphocytes and endothelial cells in first-trimester human decidua.  

PubMed

Human decidua exhibits a unique infiltrate of large granular lymphocytes (LGL) with a natural killer (NK) cell phenotype (CD56++, CD16-, CD3-). The mechanisms underlying the binding of circulating LGL to vascular endothelium in the decidua and their migration into the decidual stroma were investigated immunohistochemically in first-trimester decidua with antibodies against endothelial adhesion molecules and their counter-receptors on leukocytes. Decidual and peripheral blood LGL were also investigated by flow cytometry. In the immunohistochemical investigations, moderate to large numbers of lymphoid cells in the decidua were found to express the alpha 4 and alpha L integrin subunits, platelet endothelial cell adhesion molecule (PECAM) and intercellular adhesion molecule-1 (ICAM-1). PECAM and ICAM-1 were found on the endothelium of large numbers of decidual blood vessels of all types. Vascular cell adhesion molecule (VCAM), however, was found on the endothelium of only small to moderate numbers of arterioles and venules and a few capillaries, the latter being the main site of migration of leukocytes into the stroma. Weak staining for endothelial leukocyte adhesion molecule (ELAM) was seen only in a moderate number of blood vessels. Flow cytometry revealed expression of the alpha L integrin subunit by 72 +/- 10% and 97 +/- 3% of decidual and peripheral blood CD56+ LGL, respectively, of the alpha 4 integrin subunit by 85 +/- 7% and 90 +/- 5%, of PECAM by 40 +/- 12% and 30 +/- 15%, and of ICAM-1 by 22 +/- 10% and 1 +/- 1%.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7691868

Marzusch, K; Ruck, P; Geiselhart, A; Handgretinger, R; Dietl, J A; Kaiserling, E; Horny, H P; Vince, G; Redman, C W

1993-08-01

419

VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells  

PubMed Central

The focus of this study is the characterization of human T cell blood–brain barrier migration and corresponding molecular trafficking signatures. We examined peripheral blood and cerebrospinal fluid immune cells from patients under long-term anti–very late antigen-4 (VLA-4)/natalizumab therapy (LTNT) and from CNS specimens. LTNT patients’ cerebrospinal fluid T cells exhibited healthy central-/effector-memory ratios, but lacked CD49d and showed enhanced myeloma cell adhesion molecule (MCAM) expression. LTNT led to an increase of PSGL-1 expression on peripheral T cells. Although vascular cell adhesion molecule-1 (VLA-4 receptor) was expressed at all CNS barriers, P-selectin (PSGL-1-receptor) was mainly detected at the choroid plexus. Accordingly, in vitro experiments under physiological flow conditions using primary human endothelial cells and LTNT patients’ T cells showed increased PSGL-1–mediated rolling and residual adhesion, even under VLA-4 blockade. Adhesion of MCAM+/TH17 cells was not affected by VLA-4 blocking alone, but was abrogated when both VLA-4 and MCAM were inhibited. Consistent with these data, MCAM+ cells were detected in white matter lesions, and in gray matter of multiple sclerosis patients. Our data indicate that lymphocyte trafficking into the CNS under VLA-4 blockade can occur by using the alternative adhesion molecules, PSGL-1 and MCAM, the latter representing an exclusive pathway for TH17 cells to migrate over the blood–brain barrier. PMID:25135296

Schneider-Hohendorf, Tilman; Rossaint, Jan; Mohan, Hema; Böning, Daniel; Breuer, Johanna; Kuhlmann, Tanja; Gross, Catharina C.; Flanagan, Ken; Sorokin, Lydia; Vestweber, Dietmar; Zarbock, Alexander

2014-01-01

420

Tissue Kidney Injury Molecule-1 Expression in the Prediction of Renal Function for Several Years after Kidney Biopsy  

PubMed Central

Objectives. Retrospective study was designed to examine the importance of tissue kidney injury molecule-1 (KIM-1) expression in predicting kidney function in sixty patients (27 males) aged 34.15 ± 12.23 years with different kidney diseases over three years after kidney biopsy. Materials and Methods. Tissue KIM-1 expression was determined immunohistochemically and KIM-1 staining was scored semiquantitatively, as well as tubulointerstitialis (TIN), inflammation, atrophy, and fibrosis. Kidney function (MDRD formula) and proteinuria/day were evaluated at the time of biopsy (GFR0) and 6, 12, 24, and 36 months later. Results. Significantly positive correlations between tissue KIM-1 expression and age (r = 0.313), TIN inflammation (r = 0.456), fibrosis (r = 0.317), and proteinuria at 6 months (r = 0.394) as well as negative correlations with GFR0 (r = ?0.572), GFR6 (r = ?0.442), GFR24 (r = ?0.398), and GFR36 (r = ?0.412) were found. Meanwhile, TIN inflammation was the best predictor of all measured kidney functions during three years, while tissue KIM-1 expression (P = 0.016) was a predictor only at 6 months after biopsy. Conclusion. Tissue KIM-1 expression significantly predicts kidney function solely at 6 months after biopsy, when the effects of immune and nonimmune treatments are the strongest. PMID:24282337

Simic Ogrizovic, Sanja; Basta-Jovanovic, Gordana; Radojevic, Sanja; Pavlovic, Jelena; Kotur Stevuljevic, Jelena; Dopsaj, Violeta; Naumovic, Radomir

2013-01-01

421

Kidney Injury Molecule-1 Outperforms Traditional Biomarkers of Kidney Injury in Multi-site Preclinical Biomarker Qualification Studies  

PubMed Central

Kidney toxicity accounts for a significant percentage of morbidity and drug candidate failure. Serum creatinine (SCr) and blood urea nitrogen (BUN) have been used to monitor kidney dysfunction for over a century but these markers are insensitive and non-specific. In multi-site preclinical rat toxicology studies the diagnostic performance of urinary kidney injury molecule-1 (Kim-1) was compared to traditional biomarkers as predictors of kidney tubular histopathologic changes, currently considered the “gold standard” of nephrotoxicity. In multiple models of kidney injury, urinary Kim-1 significantly outperformed SCr and BUN. The area under the receiver operating characteristic curve for Kim-1 was between 0.91 and 0.99 as compared to 0.79 to 0.9 for BUN and 0.73 to 0.85 for SCr. Thus urinary Kim-1 is the first injury biomarker of kidney toxicity qualified by the FDA and EMEA and is expected to significantly improve kidney safety monitoring. PMID:20458318

Vaidya, Vishal S.; Ozer, Josef S.; Frank, Dieterle; Collings, Fitz B.; Ramirez, Victoria; Troth, Sean; Muniappa, Nagaraja; Thudium, Douglas; Gerhold, David; Holder, Daniel J.; Bobadilla, Norma A.; Marrer, Estelle; Perentes, Elias; Cordier, André; Vonderscher, Jacky; Maurer, Gérard; Goering, Peter L.; Sistare, Frank D.; Bonventre, Joseph V.

2010-01-01

422

Stromal interaction molecule 1 (STIM1) silencing inhibits tumor growth and promotes cell cycle arrest and apoptosis in hypopharyngeal carcinoma.  

PubMed

As an important pathway maintaining the balance of intracellular calcium (Ca(2+)), store-operated Ca(2+) entry (SOCE) is critical for cellular functions. Stromal interaction molecule 1 (STIM1), a key component of SOCE, plays a dual role as an endoplasmic reticulum Ca(2+) receptor and an SOCE exciter. Aberrant expression of STIM1 could be discovered in several human cancer cells. However, the role of STIM1 in regulating human hypopharyngeal carcinoma still remains unclear. Real-time polymerase chain reaction (PCR) was used to detect expression of STIM1 in human hypopharyngeal carcinoma cell line FaDu. STIM1 on FaDu cells was knocked down by lentiviral transduction method. The biological impacts after knocking down of STIM1 on FaDu cells were investigated in vitro and in vivo. The result of real-time PCR showed that STIM1 was expressed in FaDu cells. Lentiviral transduction efficiently downregulated the expression of STIM1 in FaDu cells at both mRNA and protein levels. Significant downregulation of STIM1 on FaDu cells inhibited cell proliferation, induced cell cycle arrest in G0/G1 phase, promoted cell apoptosis, and restrained cell growth rate. The antigrowth effect of STIM1 silencing was also discovered in FaDu hypopharyngeal tumor model. Our findings indicate that STIM1 is likely to become a new therapeutic target for hypopharyngeal carcinoma treatment. PMID:25832866

Sun, Yuanhao; Cui, Xiaobo; Wang, Jun; Wu, Shuai; Bai, Yunfei; Wang, Yaping; Wang, Boqian; Fang, Jugao

2015-05-01

423

Tissue mechanics and adhesion during embryo development.  

PubMed

During development cells interact mechanically with their microenvironment through cell-cell and cell-matrix adhesions. Many proteins involved in these adhesions serve both mechanical and signaling roles. In this review we will focus on the mechanical roles of these proteins and their complexes in transmitting force or stress from cell to cell or from cell to the extracellular matrix. As forces operate against tissues they establish tissue architecture, extracellular matrix assembly, and pattern cell shapes. As tissues become more established, adhesions play a major role integrating cells with the mechanics of their local environment. Adhesions may serve as both a molecular-specific glue, holding defined populations of cells together, and as a lubricant, allowing tissues to slide past one another. We review the biophysical principles and experimental tools used to study adhesion so that we may aid efforts to understand how adhesions guide these movements and integrate their signaling functions with mechanical function. As we conclude we review efforts to develop predictive models of adhesion that can be used to interpret experiments and guide future efforts to control and direct the process of tissue self-assembly during development. PMID:25512299

Shawky, Joseph H; Davidson, Lance A

2015-05-01

424

Adhesion of Dental Materials to Tooth Structure  

NASA Astrophysics Data System (ADS)

The understanding and proper application of the principles of adhesion has brought forth a new paradigm in the realm of esthetic dentistry. Modern restorative tooth procedures can now conserve the remaining tooth-structure and also provide for the strengthening of the tooth. Adhesive restorative techniques call for the application and curing of the dental adhesive at the interface between the tooth tissue and the filling material. Hence the success of the restoration depends largely on the integrity of this interface. The mechanism of adhesion of the bonding materials to the dental hard tissue will be discussed in this paper. There are four main steps that occur during the application of the dental adhesive to the oral hard tissues: 1) The first step is the creation of a microstructure in the tooth enamel or dentin by means of an acidic material. This can be through the application of a separate etchant or can be accomplished in situ by the adhesive/primer. This agent has to be effective in removing or modifying the proteinaceous “smear” layer, which would otherwise act as a weak boundary layer on the surface to be bonded. 2) The primer/adhesive must then be able to wet and penetrate the microstructure created in the tooth. Since the surface energies of etched enamel and that of etched dentin are different finding one material to prime both types of dental tissues can be quite challenging. 3) The ionomer types of materials, particularly those that are carboxylate ion-containing, can chemically bond with the calcium ions of the hydroxyapatite mineral. 4) Polymerization in situ allows for micromechanical interlocking of the adhesive. The importance of having the right mechanical properties of the cured adhesive layer and its role in absorbing and dissipating stresses encountered by a restored tooth will also be discussed.

Mitra, Sumita B.

2000-03-01

425

The development of low temperature curing adhesives  

NASA Technical Reports Server (NTRS)

An approach for the development of a practical low temperature (293 K-311 K/68 F-100 F) curing adhesive system based on a family of amide/ester resins was studied and demonstrated. The work was conducted on resin optimization and adhesive compounding studies. An improved preparative method was demonstrated which involved the reaction of an amine-alcohol precursor, in a DMF solution with acid chloride. Experimental studies indicated that an adhesive formulation containing aluminum powder provided the best performance when used in conjunction with a commercial primer.

Green, H. E.; Sutherland, J. D.; Hom, J. M.; Sheppard, C. H.

1975-01-01

426

Large bowel obstruction secondary to adhesive bands  

PubMed Central

Large bowel obstruction (LBO) is most commonly due to malignancy, volvulus, hernia, diverticular disease and inflammatory bowel disease. LBO due to adhesions is unusual. A literature review was conducted which revealed that only a few such cases have been reported. We report two cases of LBO secondary to adhesions in patients, one with and one without a past abdominal surgical history. We highlight that while rare, the aetiology of LBO secondary to adhesions must be considered in the differential diagnosis in patients presenting with obstructive symptoms. PMID:25650387

El-Masry, Nabil S.; Geevarghese, Ruben

2015-01-01

427

[Postoperative adhesions, the everlasting topical subject].  

PubMed

Creation of postoperative adhesions is a part of every abdominal operation. The authors analyse 320 patients operated for ileus in last 7 years. 118 patients were operated for adhesive ileus. Most common reoperations for ileus are after radical gynecological operations and inflammatory intraabdominal diseases. The creation of adhesiones depends on preoperative mechanical or chemical damage of tissues and peritoneum, bacterial infection and irradiation. The major complication of intraabdominal adhesions are disturbances of bowel function what leads to subileus or ileus. Authors present therapeutical possibilities and prefer laparoscopic operations. PMID:16689145

Bakos, E; Korcek, J; Dubaj, M; Osuský, M; Bakos, M

2006-03-01

428

Large bowel obstruction secondary to adhesive bands.  

PubMed

Large bowel obstruction (LBO) is most commonly due to malignancy, volvulus, hernia, diverticular disease and inflammatory bowel disease. LBO due to adhesions is unusual. A literature review was conducted which revealed that only a few such cases have been reported. We report two cases of LBO secondary to adhesions in patients, one with and one without a past abdominal surgical history. We highlight that while rare, the aetiology of LBO secondary to adhesions must be considered in the differential diagnosis in patients presenting with obstructive symptoms. PMID:25650387

El-Masry, Nabil S; Geevarghese, Ruben

2015-01-01

429

Adhesive bonding using variable frequency microwave energy  

DOEpatents

Methods of facilitating the adhesive bonding of various components with variable frequency microwave energy are disclosed. The time required to cure a polymeric adhesive is decreased by placing components to be bonded via the adhesive in a microwave heating apparatus having a multimode cavity and irradiated with microwaves of varying frequencies. Methods of uniformly heating various articles having conductive fibers disposed therein are provided. Microwave energy may be selectively oriented to enter an edge portion of an article having conductive fibers therein. An edge portion of an article having conductive fibers therein may be selectively shielded from microwave energy.

Lauf, Robert J. (Oak Ridge, TN); McMillan, April D. (Knoxville, TN); Paulauskas, Felix L. (Oak Ridge, TN); Fathi, Zakaryae (Cary, NC); Wei, Jianghua (Raleigh, NC)

1998-01-01

430

Adhesion of membranes via actively switched receptors  

E-print Network

We consider a theoretical model for membranes with adhesive receptors, or stickers, that are actively switched between two conformational states. In their 'on'-state, the stickers bind to ligands in an apposing membrane, whereas they do not interact with the ligands in their 'off'-state. We show that the adhesiveness of the membranes depends sensitively on the rates of the conformational switching process. This dependence is reflected in a resonance at intermediate switching rates, which can lead to large membrane separations and unbinding. Our results may provide insights into novel mechanisms for the controlled adhesion of biological or biomimetic membranes.

Bartosz Rozycki; Reinhard Lipowsky; Thomas R. Weikl

2005-12-20

431