Plant Tissue Culture in a Bag.

ERIC Educational Resources Information Center

Beck, Mike

2000-01-01

Describes the use of an oven bag as a sterile chamber for culture initiation and tissue transfer. Plant tissue culture is an ideal tool for introducing students to plants, cloning, and experimental design. Includes materials, methods, discussion, and conclusion sections. (SAH)

Transport properties of the mung bean (Vigna radiata) non-aerial hypocotyl membrane: permselectivity to hydrophilic compounds.

PubMed

Aponte, John; Baur, Peter

2014-01-01

Aerial plant surfaces are covered by a lipophilic cuticular membrane (CM) that restricts the transport of water and small solutes. Non-aerial tissues do not exhibit such a barrier. Recent data have shown that large relative to CM hydrophilic agrochemicals were able to pass at high rates through the non-aerial coleoptile. A moderately large hydrophilic solute like PEG 1000 with a mean molar volume of 782 cm(3) mol(-1) was rejected by the non-aerial hypocotyl. Uptake of smaller solutes like urea (46.5 cm(3) mol(-1) ) was fast and with 99% after 1 day. Cut-off size estimations suggest a pore size diameter below 1.5 nm. Aerial and non-aerial CM differ largely in their absolute barrier properties. This difference is related to the absence of embedded cuticular waxes in the non-aerial hypocotyl membrane, which make the CM physically dense and cause low solubility of hydrophilic solutes. The free volume for diffusion at the interface of the non-aerial hypocotyl cuticle to the environment is much larger resulting in higher penetration rates. It is suggested that diffusion through the non-aerial hypocotyl does not proceed in a real channel system with continuous aqueous phase but is more like transport through a filter with restricted diffusion in the pore openings. © 2013 Society of Chemical Industry.

Aerial thermography studies of power plant heated lakes

NASA Astrophysics Data System (ADS)

Villa-Aleman, Eliel; Garrett, Alfred J.; Kurzeja, Robert J.; Pendergast, Malcolm M.

2000-03-01

Remote sensing temperature measurements of water bodies is complicated by the temperature differences between the true surface or `skin' water and the bulk water below. Weather conditions control the reduction of the skin temperature relative to the bulk water temperature. Typical skin temperature depressions range from a few tenths of a degree Celsius to more than one degree. In this research project, the Savannah River Technology Center used aerial thermography and surface-based meteorological and water temperature measurements to study a power plant cooling lake in South Carolina. Skin and bulk water temperatures were measured simultaneously for imagery calibration and to product a database for modeling of skin temperature depressions as a function of weather and bulk water temperatures. This paper will present imagery that illustrates how the skin temperature depression was affected by different conditions in several locations on the lake and will present skin temperature modeling results.

Virtual Plant Tissue: Building Blocks for Next-Generation Plant Growth Simulation

PubMed Central

De Vos, Dirk; Dzhurakhalov, Abdiravuf; Stijven, Sean; Klosiewicz, Przemyslaw; Beemster, Gerrit T. S.; Broeckhove, Jan

2017-01-01

Motivation: Computational modeling of plant developmental processes is becoming increasingly important. Cellular resolution plant tissue simulators have been developed, yet they are typically describing physiological processes in an isolated way, strongly delimited in space and time. Results: With plant systems biology moving toward an integrative perspective on development we have built the Virtual Plant Tissue (VPTissue) package to couple functional modules or models in the same framework and across different frameworks. Multiple levels of model integration and coordination enable combining existing and new models from different sources, with diverse options in terms of input/output. Besides the core simulator the toolset also comprises a tissue editor for manipulating tissue geometry and cell, wall, and node attributes in an interactive manner. A parameter exploration tool is available to study parameter dependence of simulation results by distributing calculations over multiple systems. Availability: Virtual Plant Tissue is available as open source (EUPL license) on Bitbucket (https://bitbucket.org/vptissue/vptissue). The project has a website https://vptissue.bitbucket.io. PMID:28523006

Repressor-mediated tissue-specific gene expression in plants

DOEpatents

Meagher, Richard B [Athens, GA; Balish, Rebecca S [Oxford, OH; Tehryung, Kim [Athens, GA; McKinney, Elizabeth C [Athens, GA

2009-02-17

Plant tissue specific gene expression by way of repressor-operator complexes, has enabled outcomes including, without limitation, male sterility and engineered plants having root-specific gene expression of relevant proteins to clean environmental pollutants from soil and water. A mercury hyperaccumulation strategy requires that mercuric ion reductase coding sequence is strongly expressed. The actin promoter vector, A2pot, engineered to contain bacterial lac operator sequences, directed strong expression in all plant vegetative organs and tissues. In contrast, the expression from the A2pot construct was restricted primarily to root tissues when a modified bacterial repressor (LacIn) was coexpressed from the light-regulated rubisco small subunit promoter in above-ground tissues. Also provided are analogous repressor operator complexes for selective expression in other plant tissues, for example, to produce male sterile plants.

Hormone Profiling in Plant Tissues.

PubMed

Müller, Maren; Munné-Bosch, Sergi

2017-01-01

Plant hormones are for a long time known to act as chemical messengers in the regulation of physiological processes during a plant's life cycle, from germination to senescence. Furthermore, plant hormones simultaneously coordinate physiological responses to biotic and abiotic stresses. To study the hormonal regulation of physiological processes, three main approaches have been used (1) exogenous application of hormones, (2) correlative studies through measurements of endogenous hormone levels, and (3) use of transgenic and/or mutant plants altered in hormone metabolism or signaling. A plant hormone profiling method is useful to unravel cross talk between hormones and help unravel the hormonal regulation of physiological processes in studies using any of the aforementioned approaches. However, hormone profiling is still particularly challenging due to their very low abundance in plant tissues. In this chapter, a sensitive, rapid, and accurate method to quantify all the five "classic" classes of plant hormones plus other plant growth regulators, such as jasmonates, salicylic acid, melatonin, and brassinosteroids is described. The method includes a fast and simple extraction procedure without time consuming steps as purification or derivatization, followed by optimized ultrahigh-performance liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (UHPLC-MS/MS) analysis. This protocol facilitates the high-throughput analysis of hormone profiling and is applicable to different plant tissues.

Crossing kingdoms: Using decellularized plants as perfusable tissue engineering scaffolds.

PubMed

Gershlak, Joshua R; Hernandez, Sarah; Fontana, Gianluca; Perreault, Luke R; Hansen, Katrina J; Larson, Sara A; Binder, Bernard Y K; Dolivo, David M; Yang, Tianhong; Dominko, Tanja; Rolle, Marsha W; Weathers, Pamela J; Medina-Bolivar, Fabricio; Cramer, Carole L; Murphy, William L; Gaudette, Glenn R

2017-05-01

Despite significant advances in the fabrication of bioengineered scaffolds for tissue engineering, delivery of nutrients in complex engineered human tissues remains a challenge. By taking advantage of the similarities in the vascular structure of plant and animal tissues, we developed decellularized plant tissue as a prevascularized scaffold for tissue engineering applications. Perfusion-based decellularization was modified for different plant species, providing different geometries of scaffolding. After decellularization, plant scaffolds remained patent and able to transport microparticles. Plant scaffolds were recellularized with human endothelial cells that colonized the inner surfaces of plant vasculature. Human mesenchymal stem cells and human pluripotent stem cell derived cardiomyocytes adhered to the outer surfaces of plant scaffolds. Cardiomyocytes demonstrated contractile function and calcium handling capabilities over the course of 21 days. These data demonstrate the potential of decellularized plants as scaffolds for tissue engineering, which could ultimately provide a cost-efficient, "green" technology for regenerating large volume vascularized tissue mass. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

Trypanocidal constituents in plants 4. Withanolides from the aerial parts of Physalis angulata.

PubMed

Nagafuji, Shinya; Okabe, Hikaru; Akahane, Hiroshige; Abe, Fumiko

2004-02-01

The constituents of the aerial parts of Physalis angulata (Solanaceae) were investigated based on the plant's trypanocidal activity against epimastigotes of Trypanosoma cruzi, the etiologic agent for Chagas' disease. Four new withanolides were isolated, along with six known ones, from the active fraction. Their structures were determined by spectroscopic analysis. Trypanocidal activity against trypomastigotes, an infectious form of T. cruzi, was also estimated, as well as cytotoxic activity against human uterine carcinoma (HeLa) cells in vitro. Evaluation of trypanocidal activity using the colorimetric reagent Cell Counting Kit-8 was also examined.

Molecular and physiological mechanisms regulating tissue reunion in incised plant tissues.

PubMed

Asahina, Masashi; Satoh, Shinobu

2015-05-01

Interactions among the functionally specialized organs of higher plants ensure that the plant body develops and functions properly in response to changing environmental conditions. When an incision or grafting procedure interrupts the original organ or tissue connection, cell division is induced and tissue reunion occurs to restore physiological connections. Such activities have long been observed in grafting techniques, which are advantageous not only for agriculture and horticulture but also for basic research. To understand how this healing process is controlled and how this process is initiated and regulated at the molecular level, physiological and molecular analyses of tissue reunion have been performed using incised hypocotyls of cucumber (Cucumis sativus) and tomato (Solanum lycopersicum) and incised flowering stems of Arabidopsis thaliana. Our results suggest that leaf gibberellin and microelements from the roots are required for tissue reunion in the cortex of the cucumber and tomato incised hypocotyls. In addition, the wound-inducible hormones ethylene and jasmonic acid contribute to the regulation of the tissue reunion process in the upper and lower parts, respectively, of incised Arabidopsis stems. Ethylene and jasmonic acid modulate the expression of ANAC071 and RAP2.6L, respectively, and auxin signaling via ARF6/8 is essential for the expression of these transcription factors. In this report, we discuss recent findings regarding molecular and physiological mechanisms of the graft union and the tissue reunion process in wounded tissues of plants.

Oscillator networks with tissue-specific circadian clocks in plants.

PubMed

Inoue, Keisuke; Araki, Takashi; Endo, Motomu

2017-09-08

Many organisms rely on circadian clocks to synchronize their biological processes with the 24-h rotation of the earth. In mammals, the circadian clock consists of a central clock in the suprachiasmatic nucleus and peripheral clocks in other tissues. The central clock is tightly coupled to synchronize rhythmicity and can organize peripheral clocks through neural and hormonal signals. In contrast to mammals, it has long been assumed that the circadian clocks in each plant cell is able to be entrained by external light, and they are only weakly coupled to each other. Recently, however, several reports have demonstrated that plants have unique oscillator networks with tissue-specific circadian clocks. Here, we introduce our current view regarding tissue-specific properties and oscillator networks of plant circadian clocks. Accumulating evidence suggests that plants have multiple oscillators, which show distinct properties and reside in different tissues. A direct tissue-isolation technique and micrografting have clearly demonstrated that plants have hierarchical oscillator networks consisting of multiple tissue-specific clocks. Copyright © 2017. Published by Elsevier Ltd.

Regulatory mechanisms for specification and patterning of plant vascular tissues.

PubMed

Caño-Delgado, Ana; Lee, Ji-Young; Demura, Taku

2010-01-01

Plant vascular tissues, the conduits of water, nutrients, and small molecules, play important roles in plant growth and development. Vascular tissues have allowed plants to successfully adapt to various environmental conditions since they evolved 450 Mya. The majority of plant biomass, an important source of renewable energy, comes from the xylem of the vascular tissues. Efforts have been made to identify the underlying mechanisms of cell specification and patterning of plant vascular tissues and their proliferation. The formation of the plant vascular system is a complex process that integrates signaling and gene regulation at transcriptional and posttranscriptional levels. Recently, a wealth of molecular genetic studies and the advent of cell biology and genomic tools have enabled important progress toward understanding its underlying mechanisms. Here, we provide a comprehensive review of the cell and developmental processes of plant vascular tissue and resources recently available for studying them that will enable the discovery of new ways to develop sustainable energy using plant biomass.

Tissue-Culture Method of Cloning Rubber Plants

NASA Technical Reports Server (NTRS)

Ball, E. A.

1983-01-01

Guayule plant, a high-yield rubber plant cloned by tissue-culture method to produce multiple new plants that mature quickly. By adjusting culture medium, excised shoot tip produces up to 50 identical guayule plants. Varying concentration of cytokinin, single excised tip produces either 1 or several (up to 50) new plants.

Diffusion of biostimulators into plant tissues

NASA Astrophysics Data System (ADS)

Kolomazník, Karel; Pecha, Jiří; Friebrová, Veronika; Janáčová, Dagmar; Vašek, Vladimír

2012-09-01

Biostimulators are substances able to enhance the immune system of cultivated crops and support plant metabolism. Their utilization helps to reduce the amount of chemicals used in agriculture. To perform the desired effect, a biostimulator must be able to penetrate into the plant tissue. The time of penetration however, is limited, since the biostimulator must remain in a liquid state. This is of great importance—especially in field conditions, where the treated plants are exposed to different weather condition and other extrinsic factors. A mathematical model based on diffusion mechanisms has been elaborated to describe the biostimulator transport process from penetration of the leaves into the plant's inner tissues. By means of the effective diffusion coefficient of the prepared specific protein hydrolyzate, this model can be used to estimate the time necessary for the uptake of the minimal active amount of the biostimulator.

The role of silicon in plant tissue culture

PubMed Central

Sivanesan, Iyyakkannu; Park, Se Won

2014-01-01

Growth and morphogenesis of in vitro cultures of plant cells, tissues, and organs are greatly influenced by the composition of the culture medium. Mineral nutrients are necessary for the growth and development of plants. Several morpho-physiological disorders such as hooked leaves, hyperhydricity, fasciation, and shoot tip necrosis are often associated with the concentration of inorganic nutrient in the tissue culture medium. Silicon (Si) is the most abundant mineral element in the soil. The application of Si has been demonstrated to be beneficial for growth, development and yield of various plants and to alleviate various stresses including nutrient imbalance. Addition of Si to the tissue culture medium improves organogenesis, embryogenesis, growth traits, morphological, anatomical, and physiological characteristics of leaves, enhances tolerance to low temperature and salinity, protects cells and against metal toxicity, prevents oxidative phenolic browning and reduces the incidence of hyperhydricity in various plants. Therefore, Si possesses considerable potential for application in a wide range of plant tissue culture studies such as cryopreservation, organogenesis, micropropagation, somatic embryogenesis and secondary metabolites production. PMID:25374578

Uptake and cellular distribution, in four plant species, of fluorescently labeled mesoporous silica nanoparticles.

PubMed

Sun, Dequan; Hussain, Hashmath I; Yi, Zhifeng; Siegele, Rainer; Cresswell, Tom; Kong, Lingxue; Cahill, David M

2014-08-01

We report the uptake of MSNs into the roots and their movement to the aerial parts of four plant species and their quantification using fluorescence, TEM and proton-induced x - ray emission (micro - PIXE) elemental analysis. Monodispersed mesoporous silica nanoparticles (MSNs) of optimal size and configuration were synthesized for uptake by plant organs, tissues and cells. These monodispersed nanoparticles have a size of 20 nm with interconnected pores with an approximate diameter of 2.58 nm. There were no negative effects of MSNs on seed germination or when transported to different organs of the four plant species tested in this study. Most importantly, for the first time, a combination of confocal laser scanning microscopy, transmission electron microscopy and proton-induced X-ray emission (micro-PIXE) elemental analysis allowed the location and quantification MSNs in tissues and in cellular and sub-cellular locations. Our results show that MSNs penetrated into the roots via symplastic and apoplastic pathways and then via the conducting tissues of the xylem to the aerial parts of the plants including the stems and leaves. The translocation and widescale distribution of MSNs in plants will enable them to be used as a new delivery means for the transport of different sized biomolecules into plants.

Large-scale atlas of microarray data reveals the distinct expression landscape of different tissues in Arabidopsis

DOE Office of Scientific and Technical Information (OSTI.GOV)

He, Fei; Maslov, Sergei; Yoo, Shinjae

Here, transcriptome datasets from thousands of samples of the model plant Arabidopsis thaliana have been collectively generated by multiple individual labs. Although integration and meta-analysis of these samples has become routine in the plant research community, it is often hampered by the lack of metadata or differences in annotation styles by different labs. In this study, we carefully selected and integrated 6,057 Arabidopsis microarray expression samples from 304 experiments deposited to NCBI GEO. Metadata such as tissue type, growth condition, and developmental stage were manually curated for each sample. We then studied global expression landscape of the integrated dataset andmore » found that samples of the same tissue tend to be more similar to each other than to samples of other tissues, even in different growth conditions or developmental stages. Root has the most distinct transcriptome compared to aerial tissues, but the transcriptome of cultured root is more similar to those of aerial tissues as the former samples lost their cellular identity. Using a simple computational classification method, we showed that the tissue type of a sample can be successfully predicted based on its expression profile, opening the door for automatic metadata extraction and facilitating re-use of plant transcriptome data. As a proof of principle we applied our automated annotation pipeline to 708 RNA-seq samples from public repositories and verified accuracy of our predictions with samples’ metadata provided by authors.« less

Large-scale atlas of microarray data reveals the distinct expression landscape of different tissues in Arabidopsis

DOE PAGES

He, Fei; Maslov, Sergei; Yoo, Shinjae; ...

2016-05-25

Here, transcriptome datasets from thousands of samples of the model plant Arabidopsis thaliana have been collectively generated by multiple individual labs. Although integration and meta-analysis of these samples has become routine in the plant research community, it is often hampered by the lack of metadata or differences in annotation styles by different labs. In this study, we carefully selected and integrated 6,057 Arabidopsis microarray expression samples from 304 experiments deposited to NCBI GEO. Metadata such as tissue type, growth condition, and developmental stage were manually curated for each sample. We then studied global expression landscape of the integrated dataset andmore » found that samples of the same tissue tend to be more similar to each other than to samples of other tissues, even in different growth conditions or developmental stages. Root has the most distinct transcriptome compared to aerial tissues, but the transcriptome of cultured root is more similar to those of aerial tissues as the former samples lost their cellular identity. Using a simple computational classification method, we showed that the tissue type of a sample can be successfully predicted based on its expression profile, opening the door for automatic metadata extraction and facilitating re-use of plant transcriptome data. As a proof of principle we applied our automated annotation pipeline to 708 RNA-seq samples from public repositories and verified accuracy of our predictions with samples’ metadata provided by authors.« less

Preliminary Design of Aerial Spraying System for Microlight Aircraft

NASA Astrophysics Data System (ADS)

Omar, Zamri; Idris, Nurfazliawati; Rahim, M. Zulafif

2017-10-01

Undoubtedly agricultural is an important sector because it provides essential nutrients for human, and consequently is among the biggest sector for economic growth worldwide. It is crucial to ensure crops production is protected from any plant diseases and pests. Thus aerial spraying system on crops is developed to facilitate farmers to for crops pests control and it is very effective spraying method especially for large and hilly crop areas. However, the use of large aircraft for aerial spaying has a relatively high operational cost. Therefore, microlight aircraft is proposed to be used for crops aerial spraying works for several good reasons. In this paper, a preliminary design of aerial spraying system for microlight aircraft is proposed. Engineering design methodology is adopted in the development of the aerial sprayer and steps involved design are discussed thoroughly. A preliminary design for the microlight to be attached with an aerial spraying system is proposed.

Plant grafting: insights into tissue regeneration.

PubMed

Melnyk, Charles W

2017-02-01

For millennia, people have cut and joined different plants together through a process known as grafting. The severed tissues adhere, the cells divide and the vasculature differentiates through a remarkable process of regeneration between two genetically distinct organisms as they become one. Grafting is becoming increasingly important in horticulture where it provides an efficient means for asexual propagation. Grafting also combines desirable roots and shoots to generate chimeras that are more vigorous, more pathogen resistant and more abiotic stress resistant. Thus, it presents an elegant and efficient way to improve plant productivity in vegetables and trees using traditional techniques. Despite this horticultural importance, we are only beginning to understand how plants regenerate tissues at the graft junction. By understanding grafting better, we can shed light on fundamental regeneration pathways and the basis for self/non-self recognition. We can also better understand why many plants efficiently graft whereas others cannot, with the goal of improving grafting so as to broaden the range of grafted plants to create even more desirable chimeras. Here, I review the latest findings describing how plants graft and provide insight into future directions in this emerging field.

Fusarium oxysporum Triggers Tissue-Specific Transcriptional Reprogramming in Arabidopsis thaliana

PubMed Central

Lyons, Rebecca; Stiller, Jiri; Powell, Jonathan; Rusu, Anca; Manners, John M.; Kazan, Kemal

2015-01-01

Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including Arabidopsis thaliana. To investigate and compare transcriptional changes triggered by F. oxysporum in different Arabidopsis tissues, we infected soil-grown plants with F. oxysporum and subjected root and leaf tissue harvested at early and late timepoints to RNA-seq analyses. At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation. Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue. We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum. In the leaves, defensins and genes associated with the response to auxin, cold and senescence were strongly regulated while jasmonate biosynthesis and signalling genes were induced throughout the plant. PMID:25849296

Histochemical detection of lead and zinc in plant tissues

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tung, G.; Temple, P.J.

1975-01-01

Histochemical studies on uptake and localization of lead and zinc in plant tissues were carried out. A histochemical stain technique was developed to differentiate zinc from lead. Lead was detected in plant tissues by soaking fresh plant materials in freshly prepared sodium rhodizonate stain (0.2% Na rhodizonate acidified to pH3 with glacial acetic acid). Samples were evacuated 5 min and soaked for 30 min before embedding in the congealed stain, then sectioned with a cryostat and examined under a light microscope. Lead particles in plant tissues were stained scarlet-red. Gelatinous, proteinaceous or saccharic embedding materials normally used to prepare plantmore » sampled for sectioning in the cryostat interfered with the color reaction. Sectioning plant samples without staining whole tissues resulted in a weakened response to the stain. Color of stained sample materials were retained for several months if stored in a frozen condition. This technique was used to detect lead both inside and on the surface of plant samples collected in the vicinity of highway and industrial lead sources and to trace the pathways of lead uptake from the air or from contaminated soils. A sodium rhodizonate technique was also developed to be specific for zinc in plant tissues. Plant samples were soaked in a neutral Na-rhodizonate in phosphate buffer at pH 7.5 for observation. The color of zinc developed to produce a purplish or reddish-brown color.« less

Uniformity of environmental conditions and plant growth in a hydroponic culture system for use in a growth room with aerial CO2 control

NASA Technical Reports Server (NTRS)

Vessey, J. K.; York, E. K.; Henry, L. T.; Raper, C. D. Jr; Raper CD, J. r. (Principal Investigator)

1988-01-01

A portable system of hydroponic culture was developed that maintained temperature, pH, and nutrient concentrations of circulating nutrient solutions. The hydroponic system is used within a controlled-environment room (CER) for control of aerial environment. The CER was equipped with an auto-calibrating system for atmospheric CO2 control. The control systems for the hydroponic chambers were able to maintain acidity within +/- 0.2 pH units and the temperature with +/- 0.5 degree C. Mixing time for the 200-liter volume of solution within a hydroponic chamber was less than 12 min. The CO2 control system was able to maintain aerial concentrations within +/- 10 ppm CO2 during the light period. The only gradient found to occur within the hydroponic chambers or CER was a slight gradient in aerial temperature along the length of hydroponic chambers. Growth of soybeans [Glycine max (L.) Merr.] was characterized during a 3-week period of vegetative development by leaf number and area, plant dry weight, total N content of plants, and N depletion from the nutrient solution. The growth characteristics among populations for three hydroponic chambers within the CER were not significantly different, and the percent standard errors of means of the measurements within populations from each chamber were nearly all less than 10%. Thus, the uniformity of plant growth reflected the uniformity of environmental conditions.

Salt tolerance, salt accumulation, and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant Mesembryanthemum crystallinum.

PubMed

Agarie, Sakae; Shimoda, Toshifumi; Shimizu, Yumi; Baumann, Kathleen; Sunagawa, Haruki; Kondo, Ayumu; Ueno, Osamu; Nakahara, Teruhisa; Nose, Akihiro; Cushman, John C

2007-01-01

The aerial surfaces of the common or crystalline ice plant Mesembryanthemum crystallinum L., a halophytic, facultative crassulacean acid metabolism species, are covered with specialized trichome cells called epidermal bladder cells (EBCs). EBCs are thought to serve as a peripheral salinity and/or water storage organ to improve survival under high salinity or water deficit stress conditions. However, the exact contribution of EBCs to salt tolerance in the ice plant remains poorly understood. An M. crystallinum mutant lacking EBCs was isolated from plant collections mutagenized by fast neutron irradiation. Light and electron microscopy revealed that mutant plants lacked EBCs on all surfaces of leaves and stems. Dry weight gain of aerial parts of the mutant was almost half that of wild-type plants after 3 weeks of growth at 400 mM NaCl. The EBC mutant also showed reduced leaf succulence and leaf and stem water contents compared with wild-type plants. Aerial tissues of wild-type plants had approximately 1.5-fold higher Na(+) and Cl(-) content than the mutant grown under 400 mM NaCl for 2 weeks. Na(+) and Cl(-) partitioning into EBCs of wild-type plants resulted in lower concentrations of these ions in photosynthetically active leaf tissues than in leaves of the EBC-less mutant, particularly under conditions of high salt stress. Potassium, nitrate, and phosphate ion content decreased with incorporation of NaCl into tissues in both the wild type and the mutant, but the ratios of Na(+)/K(+) and Cl(-)/NO(3)(-)content were maintained only in the leaf and stem tissues of wild-type plants. The EBC mutant showed significant impairment in plant productivity under salt stress as evaluated by seed pod and seed number and average seed weight. These results clearly show that EBCs contribute to succulence by serving as a water storage reservoir and to salt tolerance by maintaining ion sequestration and homeostasis within photosynthetically active tissues of M. crystallinum.

Plant Tissue Culture Studies.

ERIC Educational Resources Information Center

Smith, Robert Alan

Plant tissue culture has developed into a valid botanical discipline and is considered a key area of biotechnology, but it has not been a key component of the science curriculum because of the expensive and technical nature of research in this area. This manual presents a number of activities that are relatively easy to prepare and perform. The…

Systemic signaling during plant defense.

PubMed

Kachroo, Aardra; Robin, Guillaume P

2013-08-01

Systemic acquired resistance (SAR) is a type of pathogen-induced broad-spectrum resistance in plants. During SAR, primary infection-induced rapid generation and transportation of mobile signal(s) 'prepare' the rest of the plant for subsequent infections. Several, seemingly unrelated, mobile chemical inducers of SAR have been identified, at least two of which function in a feed-back regulatory loop with a lipid transfer-like protein. Signal(s) perception in the systemic tissues relies on the presence of an intact cuticle, the waxy layer covering all aerial parts of the plant. SAR results in chromatin modifications, which prime systemic tissues for enhanced and rapid signaling derived from salicylic acid, which along with its signaling components is key for SAR induction. This review summarizes recent findings related to SAR signal generation, movement, and perception. Copyright © 2013 Elsevier Ltd. All rights reserved.

11. Photographic copy of aerial photograph dated ca. 1954; Photographer ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Photographic copy of aerial photograph dated ca. 1954; Photographer unknown; Original owned by Waterloo Courier, Waterloo, Iowa; AERIAL VIEW OF RATH COMPLEX, LOOKING WEST; BEEF KILLING BUILDING (149 AND LIVESTOCK HOLDING AREAS ARE AT LEFT CENTER; FERTILIZER PLANT/STORAGE BUILDINGS ARE AT BOTTOM OF PHOTO - Rath Packing Company, Sycamore Street between Elm & Eighteenth Streets, Waterloo, Black Hawk County, IA

TSAPA: identification of tissue-specific alternative polyadenylation sites in plants.

PubMed

Ji, Guoli; Chen, Moliang; Ye, Wenbin; Zhu, Sheng; Ye, Congting; Su, Yaru; Peng, Haonan; Wu, Xiaohui

2018-06-15

Alternative polyadenylation (APA) is now emerging as a widespread mechanism modulated tissue-specifically, which highlights the need to define tissue-specific poly(A) sites for profiling APA dynamics across tissues. We have developed an R package called TSAPA based on the machine learning model for identifying tissue-specific poly(A) sites in plants. A feature space including more than 200 features was assembled to specifically characterize poly(A) sites in plants. The classification model in TSAPA can be customized by selecting desirable features or classifiers. TSAPA is also capable of predicting tissue-specific poly(A) sites in unannotated intergenic regions. TSAPA will be a valuable addition to the community for studying dynamics of APA in plants. https://github.com/BMILAB/TSAPA. Supplementary data are available at Bioinformatics online.

A high-resolution method for the localization of proanthocyanidins in plant tissues

PubMed Central

2011-01-01

Background Histochemical staining of plant tissues with 4-dimethylaminocinnamaldehyde (DMACA) or vanillin-HCl is widely used to characterize spatial patterns of proanthocyanidin accumulation in plant tissues. These methods are limited in their ability to allow high-resolution imaging of proanthocyanidin deposits. Results Tissue embedding techniques were used in combination with DMACA staining to analyze the accumulation of proanthocyanidins in Lotus corniculatus (L.) and Trifolium repens (L.) tissues. Embedding of plant tissues in LR White or paraffin matrices, with or without DMACA staining, preserved the physical integrity of the plant tissues, allowing high-resolution imaging that facilitated cell-specific localization of proanthocyanidins. A brown coloration was seen in proanthocyanidin-producing cells when plant tissues were embedded without DMACA staining and this was likely to have been due to non-enzymatic oxidation of proanthocyanidins and the formation of colored semiquinones and quinones. Conclusions This paper presents a simple, high-resolution method for analysis of proanthocyanidin accumulation in organs, tissues and cells of two plant species with different patterns of proanthocyanidin accumulation, namely Lotus corniculatus (birdsfoot trefoil) and Trifolium repens (white clover). This technique was used to characterize cell type-specific patterns of proanthocyanidin accumulation in white clover flowers at different stages of development. PMID:21595992

Towards collaboration between unmanned aerial and ground vehicles for precision agriculture

NASA Astrophysics Data System (ADS)

Bhandari, Subodh; Raheja, Amar; Green, Robert L.; Do, Dat

2017-05-01

This paper presents the work being conducted at Cal Poly Pomona on the collaboration between unmanned aerial and ground vehicles for precision agriculture. The unmanned aerial vehicles (UAVs), equipped with multispectral/hyperspectral cameras and RGB cameras, take images of the crops while flying autonomously. The images are post processed or can be processed onboard. The processed images are used in the detection of unhealthy plants. Aerial data can be used by the UAVs and unmanned ground vehicles (UGVs) for various purposes including care of crops, harvest estimation, etc. The images can also be useful for optimized harvesting by isolating low yielding plants. These vehicles can be operated autonomously with limited or no human intervention, thereby reducing cost and limiting human exposure to agricultural chemicals. The paper discuss the autonomous UAV and UGV platforms used for the research, sensor integration, and experimental testing. Methods for ground truthing the results obtained from the UAVs will be used. The paper will also discuss equipping the UGV with a robotic arm for removing the unhealthy plants and/or weeds.

Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.

PubMed

Takeuchi, Miyuki; Takabe, Keiji; Mineyuki, Yoshinobu

2016-01-01

Cryofixation and freeze-substitution techniques provide excellent preservation of plant ultrastructure. The advantage of cryofixation is not only in structural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more detailed information on the immunoelectron-microscopic localization of molecules in the plant cell than can be obtained from chemically fixed tissues. Here, we introduce methods for immunoelectron microscopy of cryofixed and freeze-substituted plant tissues.

[Death by explosion of an aerial mine].

PubMed

Stockhausen, Sarah; Wöllner, Kirsten; Madea, Burkhard; Doberentz, Elke

2014-01-01

Civilians are rarely killed by military weapons except in times of war. In early 2014, a 50-year-old man died in an explosion of an aerial mine from the Second World War when he was crushing concrete chunks with an excavator at a recycling plant. In the burned operator's cab, the remains of a body were found on the driver's seat. The thorax and the head were missing. Still sticking in the shoe, the right foot severed at the ankle was found about 7 m from the excavator together with numerous small to tiny body parts. At autopsy, the completely disrupted, strongly charred lower torso of a male connected to the left extremities as well as a large number of small tissue fragments and calcined bones were found. According to calculations performed by the seismographical station on the basis of seismic data, only about 45-60 percent of the charge had detonated. The autopsy results illustrate all the more the massive impact of such an explosion.

High-Throughput Phenotyping of Plant Height: Comparing Unmanned Aerial Vehicles and Ground LiDAR Estimates.

PubMed

Madec, Simon; Baret, Fred; de Solan, Benoît; Thomas, Samuel; Dutartre, Dan; Jezequel, Stéphane; Hemmerlé, Matthieu; Colombeau, Gallian; Comar, Alexis

2017-01-01

The capacity of LiDAR and Unmanned Aerial Vehicles (UAVs) to provide plant height estimates as a high-throughput plant phenotyping trait was explored. An experiment over wheat genotypes conducted under well watered and water stress modalities was conducted. Frequent LiDAR measurements were performed along the growth cycle using a phénomobile unmanned ground vehicle. UAV equipped with a high resolution RGB camera was flying the experiment several times to retrieve the digital surface model from structure from motion techniques. Both techniques provide a 3D dense point cloud from which the plant height can be estimated. Plant height first defined as the z -value for which 99.5% of the points of the dense cloud are below. This provides good consistency with manual measurements of plant height (RMSE = 3.5 cm) while minimizing the variability along each microplot. Results show that LiDAR and structure from motion plant height values are always consistent. However, a slight under-estimation is observed for structure from motion techniques, in relation with the coarser spatial resolution of UAV imagery and the limited penetration capacity of structure from motion as compared to LiDAR. Very high heritability values ( H 2 > 0.90) were found for both techniques when lodging was not present. The dynamics of plant height shows that it carries pertinent information regarding the period and magnitude of the plant stress. Further, the date when the maximum plant height is reached was found to be very heritable ( H 2 > 0.88) and a good proxy of the flowering stage. Finally, the capacity of plant height as a proxy for total above ground biomass and yield is discussed.

High-Throughput Phenotyping of Plant Height: Comparing Unmanned Aerial Vehicles and Ground LiDAR Estimates

PubMed Central

Madec, Simon; Baret, Fred; de Solan, Benoît; Thomas, Samuel; Dutartre, Dan; Jezequel, Stéphane; Hemmerlé, Matthieu; Colombeau, Gallian; Comar, Alexis

2017-01-01

The capacity of LiDAR and Unmanned Aerial Vehicles (UAVs) to provide plant height estimates as a high-throughput plant phenotyping trait was explored. An experiment over wheat genotypes conducted under well watered and water stress modalities was conducted. Frequent LiDAR measurements were performed along the growth cycle using a phénomobile unmanned ground vehicle. UAV equipped with a high resolution RGB camera was flying the experiment several times to retrieve the digital surface model from structure from motion techniques. Both techniques provide a 3D dense point cloud from which the plant height can be estimated. Plant height first defined as the z-value for which 99.5% of the points of the dense cloud are below. This provides good consistency with manual measurements of plant height (RMSE = 3.5 cm) while minimizing the variability along each microplot. Results show that LiDAR and structure from motion plant height values are always consistent. However, a slight under-estimation is observed for structure from motion techniques, in relation with the coarser spatial resolution of UAV imagery and the limited penetration capacity of structure from motion as compared to LiDAR. Very high heritability values (H2> 0.90) were found for both techniques when lodging was not present. The dynamics of plant height shows that it carries pertinent information regarding the period and magnitude of the plant stress. Further, the date when the maximum plant height is reached was found to be very heritable (H2> 0.88) and a good proxy of the flowering stage. Finally, the capacity of plant height as a proxy for total above ground biomass and yield is discussed. PMID:29230229

Assessing the impacts of canopy openness and flight parameters on detecting a sub-canopy tropical invasive plant using a small unmanned aerial system

NASA Astrophysics Data System (ADS)

Perroy, Ryan L.; Sullivan, Timo; Stephenson, Nathan

2017-03-01

Small unmanned aerial systems (sUAS) have great potential to facilitate the early detection and management of invasive plants. Here we show how very high-resolution optical imagery, collected from small consumer-grade multirotor UAS platform at altitudes of 30-120 m above ground level (agl), can be used to detect individual miconia (Miconia calvescens) plants in a highly invaded tropical rainforest environment on the island of Hawai'i. The central aim of this research was to determine how overstory vegetation cover, imagery resolution, and camera look-angle impact the aerial detection of known individual miconia plants. For our finest resolution imagery (1.37 cm ground sampling distance collected at 30 m agl), we obtained a 100% detection rate for sub-canopy plants with above-crown openness values >40% and a 69% detection rate for those with >20% openness. We were unable to detect any plants with <10% above crown openness. Detection rates progressively declined with coarser spatial resolution imagery, ending in a 0% detection rate for the 120 m agl flights (ground sampling distance of 5.31 cm). The addition of forward-looking oblique imagery improved detection rates for plants below overstory vegetation, though this effect decreased with increasing flight altitude. While dense overstory canopy cover, limited flight times, and visual line of sight regulations present formidable obstacles for detecting miconia and other invasive plant species, we show that sUAS platforms carrying optical sensors can be an effective component of an integrated management plan within challenging subcanopy forest environments.

Development of germ-free plants and tissue culture

NASA Technical Reports Server (NTRS)

Venketeswaran, S.

1973-01-01

The botanical program is reported for experiments performed at the Lunar Receiving Laboratory. Papers prepared during this program are listed. The studies reported include: tissues cultured on various mediums, nutritional studies, preparation of plant cultures for Apollo 15, and pine tissue cultures.

Distribution of α-, β-, γ-, and δ-hexachlorocyclohexane in soil-plant-air system in a tea garden.

PubMed

Yi, Zhigang; Zheng, Lili; Guo, Pingping; Bi, Junqi

2013-05-01

The residue of hexachlorocyclohexane (HCH) isomers (mainly α-, β-, γ-, and δ-HCH) in the soils, plant tissues and atmosphere were measured in a typical tea garden in Fujian, a major tea-producing province in China, and this study focused on the distribution and accumulation of HCHs. HCHs could accumulate in most of the plant tissues, with the highest HCH concentration of 3.0±2.9ng/g dw in old leaves. Uptake of HCHs by the roots from soil was the possible pathway for HCHs accumulation in plants, and the accumulation was an isomer-selective process, with the highest concentration factor of 10.3 for α-HCH. The higher percentages of α- and γ-HCH in roots (28.1 percent and 43.7 percent) than those in soil (14.0% and 34.1 percent) also implied the isomer-selective accumulation of HCHs. ΣHCHs in the gaseous phase (157±97pg/m(3)) were significantly higher than those in particle phase (19±20pg/m(3)). Volatilization of HCHs from soils and uptake by the plant's aerial tissues might be the pathway for HCHs accumulation in leaves and stems, and β-HCH showed the highest accumulation capacity in young leaves. The percentage distribution pattern of the dust on plant leaves were similar to that in soils, suggesting that the dust on the leaves were mainly from the soils. High γ-HCH concentrations and low α-/γ-HCH ratios in plant's aerial tissues suggested the input of lindane in tea garden. Copyright © 2013 Elsevier Inc. All rights reserved.

Phytotoxic activity and chemical composition of Cassia absus seeds and aerial parts.

PubMed

Zribi, I; Sbai, H; Ghezal, N; Richard, G; Trisman, D; Fauconnier, M L; Haouala, R

2017-12-01

The present study was conducted to assess the phytotoxic potential and the phytochemical composition of Cassia absus. Aqueous extracts caused significant reduction in root growth of Lactuca sativa. Seed extract was more effective than aerial part extract. Successive extractions of this plant were performed using solvents with increasing polarities. The methanolic seed extract exerted strong phytotoxic effect on seedling growth, followed by petroleum ether extract of the aerial part. The phytochemical investigation showed that among the organic extracts, methanol extracts of seeds and aerial parts contained the highest amounts of total phenolics and proanthocyanidins. Seeds were rich in linoleic acid followed by palmitic acids. Palmitic, stearic and arachidic acids were the major fatty acids in aerial parts. HPLC-DAD analysis of the methanolic extracts revealed the presence of luteolin in C. absus aerial parts.

Aerial Explorers

NASA Technical Reports Server (NTRS)

Young, Larry A.; Pisanich, Greg; Ippolito, Corey

2005-01-01

This paper presents recent results from a mission architecture study of planetary aerial explorers. In this study, several mission scenarios were developed in simulation and evaluated on success in meeting mission goals. This aerial explorer mission architecture study is unique in comparison with previous Mars airplane research activities. The study examines how aerial vehicles can find and gain access to otherwise inaccessible terrain features of interest. The aerial explorer also engages in a high-level of (indirect) surface interaction, despite not typically being able to takeoff and land or to engage in multiple flights/sorties. To achieve this goal, a new mission paradigm is proposed: aerial explorers should be considered as an additional element in the overall Entry, Descent, Landing System (EDLS) process. Further, aerial vehicles should be considered primarily as carrier/utility platforms whose purpose is to deliver air-deployed sensors and robotic devices, or symbiotes, to those high-value terrain features of interest.

Effects of perchlorate on growth of four wetland plants and its accumulation in plant tissues.

PubMed

He, Hongzhi; Gao, Haishuo; Chen, Guikui; Li, Huashou; Lin, Hai; Shu, Zhenzhen

2013-10-01

Perchlorate contamination in water is of concern because of uncertainties about toxicity and health effects, impact on ecosystems, and possible indirect exposure pathways to humans. Therefore, it is very important to investigate the ecotoxicology of perchlorate and to screen plant species for phytoremediation. Effects of perchlorate (20, 200, and 500 mg/L) on the growth of four wetland plants (Eichhornia crassipes, Acorus calamus L., Thalia dealbata, and Canna indica) as well as its accumulation in different plant tissues were investigated through water culture experiments. Twenty milligrams per liter of perchlorate had no significant effects on height, root length, aboveground part weight, root weight, and oxidizing power of roots of four plants, except A. calamus, and increasing concentrations of perchlorate showed that out of the four wetland plants, only A. calamus had a significant (p<0.05) dose-dependent decrease in these parameters. When treated with 500 mg/L perchlorate, these parameters and chlorophyll content in the leaf of plants showed significant decline contrasted to control groups, except the root length of E. crassipes and C. indica. The order of inhibition rates of perchlorate on root length, aboveground part weight and root weight, and oxidizing power of roots was: A. calamus > C. indica > T. dealbata > E. crassipes and on chlorophyll content in the leaf it was: A. calamus > T. dealbata > C. indica > E. crassipes. The higher the concentration of perchlorate used, the higher the amount of perchlorate accumulation in plants. Perchlorate accumulation in aboveground tissues was much higher than that in underground tissues and leaf was the main tissue for perchlorate accumulation. The order of perchlorate accumulation content and the bioconcentration factor in leaf of four plants was: E. crassipes > C. indica > T. dealbata > A. calamus. Therefore, E. crassipes might be an ideal plant with high tolerance ability and accumulation ability for constructing

Application of plant cell and tissue culture for the production of phytochemicals in medicinal plants.

PubMed

Pant, Bijaya

2014-01-01

Approximately 80% of the world inhabitants depend on the medicinal plants in the form of traditional formulations for their primary health care system well as in the treatment of a number of diseases since the ancient time. Many commercially used drugs have come from the information of indigenous knowledge of plants and their folk uses. Linking of the indigenous knowledge of medicinal plants to modern research activities provides a new reliable approach, for the discovery of novel drugs much more effectively than with random collection. Increase in population and increasing demand of plant products along with illegal trade are causing depletion of medicinal plants and many are threatened in natural habitat. Plant tissue culture technique has proved potential alternative for the production of desirable bioactive components from plants, to produce the enough amounts of plant material that is needed and for the conservation of threatened species. Different plant tissue culture systems have been extensively studied to improve and enhance the production of plant chemicals in various medicinal plants.

In vitro plant tissue culture: means for production of biological active compounds.

PubMed

Espinosa-Leal, Claudia A; Puente-Garza, César A; García-Lara, Silverio

2018-05-07

Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

Environmental geophysics and sequential aerial photo study at Sunfish and Marsden Lakes, Twin Cities Army Ammunition Plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Padar, C.A.; McGinnis, L.D.; Thompson, M.D.

1995-08-01

Geophysical studies at Site H of Twin Cities Army Ammunition Plant have delineated specific areas of dumping and waste disposal. Anomalous areas noted in the geophysical data sets have been correlated with features visible in a chronological sequence of aerial photos. The photos aid in dating the anthropogenic changes and in interpreting the geophysical anomalies observed at Site H and across Sunfish Lake. Specifically, two burn cages and what has been interpreted as their surrounding debris have been delineated. The areal extent of another waste site has been defined in the southwest corner of Area H-1. Depth estimates to themore » top of the Area H-1 anomalies show that the anomalies lie below lake level, indicative of dumping directly into Sunfish Lake. Except for these areas along the northwestern shore, there is no evidence of waste disposal along the shoreline or within the present-day lake margins. Magnetic, electromagnetic, and ground-penetrating-radar data have pinpointed the locations of mounds, observable in aerial photos, around the first burn cage. The second burn cage and its surrounding area have also been clearly defined from aerial photos, with support from further geophysical data. Additional analysis of the data has yielded volumetric estimates of the amount of material that would need removal in the event of excavation of the anomalous areas. Magnetic and electromagnetic profiles were also run across Marsden Lake. On the basis of these data, it has been concluded that no large-scale dumping has occurred in or around Marsden Lake.« less

AERIAL VIEW OF BUILDING 460, LOOKING NORTHEAST. THE BUILDING WAS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

AERIAL VIEW OF BUILDING 460, LOOKING NORTHEAST. THE BUILDING WAS CONSTRUCTED TO CONSOLIDATE ALL NON-NUCLEAR MANUFACTURING AT THE ROCKY FLATS PLANT INTO ONE FACILITY. (6/13/85) - Rocky Flats Plant, Stainless Steel & Non-Nuclear Components Manufacturing, Southeast corner of intersection of Cottonwood & Third Avenues, Golden, Jefferson County, CO

Classification of explosives transformation products in plant tissue

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larson, S.L.; Jones, R.P.; Escalon, L.

Explosives contamination in surface or groundwater used for the irrigation of food crops and phytoremediation of explosives-contaminated soil or water using plant-assisted biodegradation have brought about concerns as to the fate of explosives in plants. Liquid scintillation counting, high-performance liquid chromatography, and gel permeation chromatography were utilized to characterize explosives (hexahydro-1,3,5-trinitro-1,3,5-triazine and trinitrotoluene) and their metabolites in plant tissues obtained from three separate studies. Analyzing tissues of yellow nutsedge (Cyperus esculentus), corn (Zea mays), lettuce (Lacuta sativa), tomato (Lyopersicum esculentum), radish (Raphanus sativus), and parrot feather (Myriophyllum aquaticum) from three studies where exposure to explosives at nontoxic levels occurred showedmore » that extensive transformation of the explosive contaminant occurred, variations were noted in uptake and transformation between terrestrial and aquatic plants, the products had significantly higher polarity and water solubility than the parent compounds, and the molecular sizes of the transformation products were significantly greater than those of the parent compounds.« less

Exploring the plant-associated bacterial communities in Medicago sativa L

PubMed Central

2012-01-01

Background Plant-associated bacterial communities caught the attention of several investigators which study the relationships between plants and soil and the potential application of selected bacterial species in crop improvement and protection. Medicago sativa L. is a legume crop of high economic importance as forage in temperate areas and one of the most popular model plants for investigations on the symbiosis with nitrogen fixing rhizobia (mainly belonging to the alphaproteobacterial species Sinorhizobium meliloti). However, despite its importance, no studies have been carried out looking at the total bacterial community associated with the plant. In this work we explored for the first time the total bacterial community associated with M. sativa plants grown in mesocosms conditions, looking at a wide taxonomic spectrum, from the class to the single species (S. meliloti) level. Results Results, obtained by using Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis, quantitative PCR and sequencing of 16 S rRNA gene libraries, showed a high taxonomic diversity as well as a dominance by members of the class Alphaproteobacteria in plant tissues. Within Alphaproteobacteria the families Sphingomonadaceae and Methylobacteriaceae were abundant inside plant tissues, while soil Alphaproteobacteria were represented by the families of Hyphomicrobiaceae, Methylocystaceae, Bradyirhizobiaceae and Caulobacteraceae. At the single species level, we were able to detect the presence of S. meliloti populations in aerial tissues, nodules and soil. An analysis of population diversity on nodules and soil showed a relatively low sharing of haplotypes (30-40%) between the two environments and between replicate mesocosms, suggesting drift as main force shaping S. meliloti population at least in this system. Conclusions In this work we shed some light on the bacterial communities associated with M. sativa plants, showing that Alphaproteobacteria may constitute an important

Triacylglycerol Metabolism, Function, and Accumulation in Plant Vegetative Tissues.

PubMed

Xu, Changcheng; Shanklin, John

2016-04-29

Oils in the form of triacylglycerols are the most abundant energy-dense storage compounds in eukaryotes, and their metabolism plays a key role in cellular energy balance, lipid homeostasis, growth, and maintenance. Plants accumulate oils primarily in seeds and fruits. Plant oils are used for food and feed and, increasingly, as feedstocks for biodiesel and industrial chemicals. Although plant vegetative tissues do not accumulate significant levels of triacylglycerols, they possess a high capacity for their synthesis, storage, and metabolism. The development of plants that accumulate oil in vegetative tissues presents an opportunity for expanded production of triacylglycerols as a renewable and sustainable bioenergy source. Here, we review recent progress in the understanding of triacylglycerol synthesis, turnover, storage, and function in leaves and discuss emerging genetic engineering strategies targeted at enhancing triacylglycerol accumulation in biomass crops. Such plants could potentially be modified to produce oleochemical feedstocks or nutraceuticals.

69. AERIAL VIEW OF EAST END OF PARKWAY, LOOKING WEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

69. AERIAL VIEW OF EAST END OF PARKWAY, LOOKING WEST TOWARD BRIDGE AND SIKORSKY HELICOPTER PLANT. - Merritt Parkway, Beginning in Greenwich & running 38 miles to Stratford, Greenwich, Fairfield County, CT

64. AERIAL VIEW OF EAST END OF PARKWAY, LOOKING WEST, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

64. AERIAL VIEW OF EAST END OF PARKWAY, LOOKING WEST, SIKORSKY HELICOPTER PLANT TO THE RIGHT. - Merritt Parkway, Beginning in Greenwich & running 38 miles to Stratford, Greenwich, Fairfield County, CT

Response of plant nutrient stoichiometry to fertilization varied with plant tissues in a tropical forest

PubMed Central

Mo, Qifeng; Zou, Bi; Li, Yingwen; Chen, Yao; Zhang, Weixin; Mao, Rong; Ding, Yongzhen; Wang, Jun; Lu, Xiankai; Li, Xiaobo; Tang, Jianwu; Li, Zhian; Wang, Faming

2015-01-01

Plant N:P ratios are widely used as indices of nutrient limitation in terrestrial ecosystems, but the response of these metrics in different plant tissues to altered N and P availability and their interactions remains largely unclear. We evaluated changes in N and P concentrations, N:P ratios of new leaves (<1 yr), older leaves (>1 yr), stems and mixed fine roots of seven species after 3-years of an N and P addition experiment in a tropical forest. Nitrogen addition only increased fine root N concentrations. P addition increased P concentrations among all tissues. The N × P interaction reduced leaf and stem P concentrations, suggesting a negative effect of N addition on P concentrations under P addition. The reliability of using nutrient ratios as indices of soil nutrient availability varied with tissues: the stoichiometric metrics of stems and older leaves were more responsive indicators of changed soil nutrient availability than those of new leaves and fine roots. However, leaf N:P ratios can be a useful indicator of inter-specific variation in plant response to nutrients availability. This study suggests that older leaf is a better choice than other tissues in the assessment of soil nutrient status and predicting plant response to altered nutrients using nutrients ratios. PMID:26416169

Triacylglycerol Metabolism, Function, and Accumulation in Plant Vegetative Tissues

DOE PAGES

Xu, Changcheng; Shanklin, John

2016-02-03

One of the most abundant energy-dense storage compounds in eukaryotes are oils in the form of triacylglycerols , and their metabolism plays a key role in cellular energy balance, lipid homeostasis, growth, and maintenance. Plants accumulate oils primarily in seeds and fruits. Moreover, plant oils are used for food and feed and, increasingly, as feedstocks for biodiesel and industrial chemicals. Although plant vegetative tissues do not accumulate significant levels of triacylglycerols, they possess a high capacity for their synthesis, storage, and metabolism. The development of plants that accumulate oil in vegetative tissues presents an opportunity for expanded production of triacylglycerolsmore » as a renewable and sustainable bioenergy source. We review recent progress in the understanding of triacylglycerol synthesis, turnover, storage, and function in leaves and discuss emerging genetic engineering strategies targeted at enhancing triacylglycerol accumulation in biomass crops. Such plants could potentially be modified to produce oleochemical feedstocks or nutraceuticals.« less

Antioxidant property of aerial parts and root of Phyllanthus fraternus Webster, an important medicinal plant.

PubMed

Upadhyay, Richa; Chaurasia, Jitendra Kumar; Tiwari, Kavindra Nath; Singh, Karuna

2014-01-01

In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe³⁺-Fe⁺ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.

11. AERIAL VIEW OF CROSSCUT FACILITY, LOOKING NORTH. THE HYDRO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. AERIAL VIEW OF CROSSCUT FACILITY, LOOKING NORTH. THE HYDRO PLANT CENTER SITS ON THE EDGE OF RAVINE WHICH IS ACTUALLY THE BEGINNING OF THE GRAND CANAL. THE CROSS-CUT STEAM PLANT IS THE LARGE WHITE BUILDING JUST WEST OF THE HYDRO PLANT, WITH THE TRANSMISSION SWITCHYARD IN BETWEEN. THE OTHER BUILDINGS ARE SALT RIVER PROJECT FABRICATION AND EQUIPMENT SHOPS Photographer unknown, August 22, 1958 - Cross Cut Hydro Plant, North Side of Salt River, Tempe, Maricopa County, AZ

AERIAL SHOWING COMPLETED REMOTE ANALYTICAL FACILITY (CPP627) ADJOINING FUEL PROCESSING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

AERIAL SHOWING COMPLETED REMOTE ANALYTICAL FACILITY (CPP-627) ADJOINING FUEL PROCESSING BUILDING AND EXCAVATION FOR HOT PILOT PLANT TO RIGHT (CPP-640). INL PHOTO NUMBER NRTS-60-1221. J. Anderson, Photographer, 3/22/1960 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

Multiscale detection of sulfur cinquefoil using aerial photography.

Treesearch

Bridgett J. Naylor; Bryan A. Endress; Catherine G. Parks

2005-01-01

We evaluated the effectiveness of natural color aerial photography as a tool to improve detection, monitoring, and mapping of sulfur cinquefoil (Potentilla recta L.) infestations. Sulfur cinquefoil is an exotic perennial plant invading interior Pacific Northwest rangelands. Because sulfur cinquefoil produces distinctive pale yellow flowers, we...

4. AERIAL VIEW, LOOKING SOUTHSOUTHWEST, OF BUILDING 371 GROUND FLOOR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. AERIAL VIEW, LOOKING SOUTH-SOUTHWEST, OF BUILDING 371 GROUND FLOOR UNDER CONSTRUCTION. THE GROUND FLOOR, WHICH CONTAINS THE MAJORITY OF THE PLUTONIUM RECOVERY PROCESSING EQUIPMENT, IS DIVIDED INTO COMPARTMENTS BY FIREWALLS, AIRLOCKS, AND USE OF NEGATIVE AIR PRESSURE. (1/7/75) - Rocky Flats Plant, Plutonium Recovery Facility, Northwest portion of Rocky Flats Plant, Golden, Jefferson County, CO

Floating aerial LED signage based on aerial imaging by retro-reflection (AIRR).

PubMed

Yamamoto, Hirotsugu; Tomiyama, Yuka; Suyama, Shiro

2014-11-03

We propose a floating aerial LED signage technique by utilizing retro-reflection. The proposed display is composed of LEDs, a half mirror, and retro-reflective sheeting. Directivity of the aerial image formation and size of the aerial image have been investigated. Furthermore, a floating aerial LED sign has been successfully formed in free space.

Histochemical detection of lead in plant tissues

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tung, G.; Temple, P.J.

1996-06-01

A histochemical staining technique using sodium rhodizonate was developed for detecting lead in living or preserved plant tissues. Sodium rhodizonate formed a bright scarlet-red precipitate with lead at pH 3.0, but showed no significant color responses with other metals. The precipitation of lead by this staining technique was confirmed by detection of lead in the red-stained precipitate with electron microscopy X-ray analysis. This histochemical technique for lead provided rapid, quantifiable, and unambiguous evidence for the accumulation and localization of lead in plant tissues. Soil-borne lead accumulated primarily in the roots, although at high concentrations, lead also accumulated at the endsmore » of transpirational streams, particularly at hydathodes, trichomes, and the termini of xylem streams. Lead deposited from the atmosphere accumulated on the surface of conifer foliage and also appeared in or on cell walls of various internal cells and tissues. Lead concentrations in foliage and the color intensity of the stained deposits in spruce foliage decreased with distance from the lead source and increased with age of needles. No evidence of lead deposition inside cell contents was observed by this stain.« less

Tropine forming tropinone reductase gene from Withania somnifera (Ashwagandha): biochemical characteristics of the recombinant enzyme and novel physiological overtones of tissue-wide gene expression patterns.

PubMed

Kushwaha, Amit Kumar; Sangwan, Neelam Singh; Trivedi, Prabodh Kumar; Negi, Arvind Singh; Misra, Laxminarain; Sangwan, Rajender Singh

2013-01-01

Withania somnifera is one of the most reputed medicinal plants of Indian systems of medicine synthesizing diverse types of secondary metabolites such as withanolides, alkaloids, withanamides etc. Present study comprises cloning and E. coli over-expression of a tropinone reductase gene (WsTR-I) from W. somnifera, and elucidation of biochemical characteristics and physiological role of tropinone reductase enzyme in tropane alkaloid biosynthesis in aerial tissues of the plant. The recombinant enzyme was demonstrated to catalyze NADPH-dependent tropinone to tropine conversion step in tropane metabolism, through TLC, GC and GC-MS-MS analyses of the reaction product. The functionally active homodimeric ~60 kDa enzyme catalyzed the reaction in reversible manner at optimum pH 6.7. Catalytic kinetics of the enzyme favoured its forward reaction (tropine formation). Comparative 3-D models of landscape of the enzyme active site contours and tropinone binding site were also developed. Tissue-wide and ontogenic stage-wise assessment of WsTR-I transcript levels revealed constitutive expression of the gene with relatively lower abundance in berries and young leaves. The tissue profiles of WsTR-I expression matched those of tropine levels. The data suggest that, in W. somnifera, aerial tissues as well possess tropane alkaloid biosynthetic competence. In vivo feeding of U-[(14)C]-sucrose to orphan shoot (twigs) and [(14)C]-chasing revealed substantial radiolabel incorporation in tropinone and tropine, confirming the de novo synthesizing ability of the aerial tissues. This inherent independent ability heralds a conceptual novelty in the backdrop of classical view that these tissues acquire the alkaloids through transportation from roots rather than synthesis. The TR-I gene expression was found to be up-regulated on exposure to signal molecules (methyl jasmonate and salicylic acid) and on mechanical injury. The enzyme's catalytic and structural properties as well as gene expression

4. Aerial view of Whitsett intake (lower right), Parker Dam ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Aerial view of Whitsett intake (lower right), Parker Dam and village (left), Gene Wash Reservoir, Gene Pump Plant and village (right). - Parker Dam, Spanning Colorado River between AZ & CA, Parker, La Paz County, AZ

Effect of lunar materials on plant tissue culture.

NASA Technical Reports Server (NTRS)

Walkinshaw, C. H.; Venketeswaran, S.; Baur, P. S.; Croley, T. E.; Scholes, V. E.; Weete, J. D.; Halliwell, R. S.; Hall, R. H.

1973-01-01

Lunar material collected during the Apollo 11, 12, 14, and 15 missions has been used to treat 12 species of higher plant tissue cultures. Biochemical and morphological studies have been conducted on several of these species. Tobacco tissue cultures treated with 0.22 g of lunar material exhibited increased greening more complex chloroplasts, less cytoplasmic vacuolation and greater vesiculation. Pine tissue cultures reacted to treatment by an increased deposition of tannin-like materials. The percentage of dry weight and soluble protein was increased in cultures treated with either lunar or terrestrial rock materials.

Life cycle specialization of filamentous pathogens - colonization and reproduction in plant tissues.

PubMed

Haueisen, Janine; Stukenbrock, Eva H

2016-08-01

Filamentous plant pathogens explore host tissues to obtain nutrients for growth and reproduction. Diverse strategies for tissue invasion, defense manipulation, and colonization of inter and intra-cellular spaces have evolved. Most research has focused on effector molecules, which are secreted to manipulate plant immunity and facilitate infection. Effector genes are often found to evolve rapidly in response to the antagonistic host-pathogen co-evolution but other traits are also subject to adaptive evolution during specialization to the anatomy, biochemistry and ecology of different plant hosts. Although not directly related to virulence, these traits are important components of specialization but little is known about them. We present and discuss specific life cycle traits that facilitate exploration of plant tissues and underline the importance of increasing our insight into the biology of plant pathogens. Copyright © 2016. Published by Elsevier Ltd.

Plant tissue and the color infrared record

NASA Technical Reports Server (NTRS)

Pease, R. W.

1969-01-01

Green plant tissue should not be considered as having a uniguely high near-infrared reflectance but rather a low visual reflectance. Leaf tissue without chloroplasts appears to reflect well both visual and near infrared wavelengths. The sensitometry of color infrared film is such that a spectral imbalance strongly favoring infrared reflection is necessary to yield a red record. It is the absorption of visual light by chlorophyll that creates the imbalance that makes the typical red record for plants possible. Reflectance measurements of leaves that have been chemically blanched or which have gone into natural chloride decline strongly suggests that it is the rise in the visual reflectance that is most important in removing the imbalance and degrading the red CIR record. The role of water in leaves appears to be that of rendering epidermal membranes translucent so that the underlying chlorophyll controls the reflection rather than the leaf surface.

In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections.

PubMed

Ellison, Mitchell A; McMahon, Michael B; Bonde, Morris R; Palmer, Cristi L; Luster, Douglas G

2016-01-01

Rust fungi are obligate pathogens with multiple life stages often including different spore types and multiple plant hosts. While individual rust pathogens are often associated with specific plants, a wide range of plant species are infected with rust fungi. To study the interactions between these important pathogenic fungi and their host plants, one must be able to differentiate fungal tissue from plant tissue. This can be accomplished using the In situ hybridization (ISH) protocol described here. To validate reproducibility using the ISH protocol, samples of Chrysanthemum × morifolium infected with Puccinia horiana, Gladiolus × hortulanus infected with Uromyces transversalis and Glycine max infected with Phakopsora pachyrhizi were tested alongside uninfected leaf tissue samples. The results of these tests show that this technique clearly distinguishes between rust pathogens and their respective host plant tissues. This ISH protocol is applicable to rust fungi and potentially other plant pathogenic fungi as well. It has been shown here that this protocol can be applied to pathogens from different genera of rust fungi with no background staining of plant tissue. We encourage the use of this protocol for the study of plant pathogenic fungi in paraffin embedded sections of host plant tissue.

Airborne remote sensing assessment of the damage to cotton caused by spray drift from aerially applied glyphosate through spray deposition measurements

USDA-ARS?s Scientific Manuscript database

Off-target drift of aerially applied glyphosate can cause plant injury, which is of great concern to farmers and aerial applicators. To determine the extent of crop injury due to near-field drift, an experiment was conducted with a single aerial application of glyphosate. For identification of the d...

52. Neg. No.none, ca. 1950's, PhotographerUnknown, AERIAL VIEWS OF THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

52. Neg. No.-none, ca. 1950's, Photographer-Unknown, AERIAL VIEWS OF THE FORD MOTOR COMPANY ASSEMBLY PLANT, SOMETIME AFTER THE ADDITION OF THE NORTHERN WING - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

AERIAL VIEW OF MAIN PROCESSING BUILDING SHOWING CONSTRUCTION PROGRESS AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

AERIAL VIEW OF MAIN PROCESSING BUILDING SHOWING CONSTRUCTION PROGRESS AND EXCAVATION FOR LABORATORY ON LEFT. INL PHOTO NUMBER NRTS-51-1759. Unknown Photographer, 3/28/1951 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

Aerial Images and Convolutional Neural Network for Cotton Bloom Detection.

PubMed

Xu, Rui; Li, Changying; Paterson, Andrew H; Jiang, Yu; Sun, Shangpeng; Robertson, Jon S

2017-01-01

Monitoring flower development can provide useful information for production management, estimating yield and selecting specific genotypes of crops. The main goal of this study was to develop a methodology to detect and count cotton flowers, or blooms, using color images acquired by an unmanned aerial system. The aerial images were collected from two test fields in 4 days. A convolutional neural network (CNN) was designed and trained to detect cotton blooms in raw images, and their 3D locations were calculated using the dense point cloud constructed from the aerial images with the structure from motion method. The quality of the dense point cloud was analyzed and plots with poor quality were excluded from data analysis. A constrained clustering algorithm was developed to register the same bloom detected from different images based on the 3D location of the bloom. The accuracy and incompleteness of the dense point cloud were analyzed because they affected the accuracy of the 3D location of the blooms and thus the accuracy of the bloom registration result. The constrained clustering algorithm was validated using simulated data, showing good efficiency and accuracy. The bloom count from the proposed method was comparable with the number counted manually with an error of -4 to 3 blooms for the field with a single plant per plot. However, more plots were underestimated in the field with multiple plants per plot due to hidden blooms that were not captured by the aerial images. The proposed methodology provides a high-throughput method to continuously monitor the flowering progress of cotton.

Traits, properties, and performance: how woody plants combine hydraulic and mechanical functions in a cell, tissue, or whole plant.

PubMed

Lachenbruch, Barbara; McCulloh, Katherine A

2014-12-01

This review presents a framework for evaluating how cells, tissues, organs, and whole plants perform both hydraulic and mechanical functions. The morphological alterations that affect dual functionality are varied: individual cells can have altered morphology; tissues can have altered partitioning to functions or altered cell alignment; and organs and whole plants can differ in their allocation to different tissues, or in the geometric distribution of the tissues they have. A hierarchical model emphasizes that morphological traits influence the hydraulic or mechanical properties; the properties, combined with the plant unit's environment, then influence the performance of that plant unit. As a special case, we discuss the mechanisms by which the proxy property wood density has strong correlations to performance but without direct causality. Traits and properties influence multiple aspects of performance, and there can be mutual compensations such that similar performance occurs. This compensation emphasizes that natural selection acts on, and a plant's viability is determined by, its performance, rather than its contributing traits and properties. Continued research on the relationships among traits, and on their effects on multiple aspects of performance, will help us better predict, manage, and select plant material for success under multiple stresses in the future. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Distribution pathways of hexachlorocyclohexane isomers in a soil-plant-air system. A case study with Cynara scolymus L. and Erica sp. plants grown in a contaminated site.

PubMed

Pereira, R Calvelo; Monterroso, C; Macías, F; Camps-Arbestain, M

2008-09-01

This study focuses on the main routes of distribution and accumulation of different hexachlorocyclohexane (HCH) isomers (mainly alpha-, beta-, gamma- and delta-HCH) in a soil-plant-air system. A field assay was carried out with two plant species, Cynara scolymus L. and Erica sp., which were planted either: (i) directly in the HCH-contaminated soil; or (ii) in pots filled with uncontaminated soil, which were placed in the HCH-contaminated soil. Both plant species accumulated HCH in their tissues, with relatively higher accumulation in above-ground biomass than in roots. The beta-HCH isomer was the main isomer in all plant tissues. Adsorption of HCH by the roots from contaminated soil (soil-->root pathway) and adsorption through the aerial biomass from either the surrounding air, following volatilization of the contaminant (soil-->air-->shoot pathway), and/or contact with air-suspended particles contaminated with HCH (soil particles-->shoot pathway) were the main mechanisms of accumulation. These results may have important implications for the use of plants for reducing the transfer of contaminants via the atmosphere.

[Effective productions of plant secondary metabolites having antitumor activity by plant cell and tissue cultures].

PubMed

Taniguchi, Shoko

2005-06-01

Methods for the effective production of plant secondary metabolites with antitumor activity using plant cell and tissue cultures were developed. The factors in tannin productivity were investigated using culture strains producing different types of hydrolyzable tannins, i.e., gallotannins (mixture of galloylglucoses), ellagi-, and dehydroellagitannins. Production of ellagi- and dehydroellagitannins was affected by the concentrations and ratio of nitrogen sources in the medium. The formation of oligomeric ellagitannins in shoots of Oenothera tetraptera was correlated with the differentiation of tissues. Cultured cells of Eriobotrya japonica producing ursane- and oleanane-type triterpenes with antitumor activities were also established.

Plant tissue-based chemiluminescence biosensor for ethanol.

PubMed

Huang, Yuming; Wu, Fangqiong

2006-07-01

A plant tissue-based chemiluminescence biosensor for ethanol based on using mushroom (Agaricus bisporus) tissue as the recognition element is proposed in this paper. The principle for ethanol sensing relies on the luminol-potassium hexacyanoferrate(III)-hydrogen peroxide transducer reaction, in which hydrogen peroxide is produced from the ethanol enzymatic catalytic oxidation by oxygen under the catalysis of alcohol oxidase in the tissue column. Under optimum conditions, the method allowed the measurement of ethanol in the range of 0.001 - 2 mmol/l with a detection limit (3 sigma) of 0.2 micromol/l. The relative standard deviation (RSD) was 4.14% (n = 11) for 0.05 mmol/l ethanol. The proposed method has been applied to the determination of ethanol in biological fluids and beverages with satisfactory results.

Alterations in Rubisco activity and in stomatal behavior induce a daily rhythm in photosynthesis of aerial leaves in the amphibious-plant Nuphar lutea.

PubMed

Snir, Ainit; Gurevitz, Michael; Marcus, Yehouda

2006-12-01

Nuphar lutea is an amphibious plant with submerged and aerial foliage, which raises the question how do both leaf types perform photosynthetically in two different environments. We found that the aerial leaves function like terrestrial sun-leaves in that their photosynthetic capability was high and saturated under high irradiance (ca. 1,500 mumol photons m(-2) s(-1)). We show that stomatal opening and Rubisco activity in these leaves co-limited photosynthesis at saturating irradiance fluctuating in a daily rhythm. In the morning, sunlight stimulated stomatal opening, Rubisco synthesis, and the neutralization of a night-accumulated Rubisco inhibitor. Consequently, the light-saturated quantum efficiency and rate of photosynthesis increased 10-fold by midday. During the afternoon, gradual closure of the stomata and a decrease in Rubisco content reduced the light-saturated photosynthetic rate. However, at limited irradiance, stomatal behavior and Rubisco content had only a marginal effect on the photosynthetic rate, which did not change during the day. In contrast to the aerial leaves, the photosynthesis rate of the submerged leaves, adapted to a shaded environment, was saturated under lower irradiance. The light-saturated quantum efficiency of these leaves was much lower and did not change during the day. Due to their low photosynthetic affinity for CO(2) (35 muM) and inability to utilize other inorganic carbon species, their photosynthetic rate at air-equilibrated water was CO(2)-limited. These results reveal differences in the photosynthetic performance of the two types of Nuphar leaves and unravel how photosynthetic daily rhythm in the aerial leaves is controlled.

In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections

USDA-ARS?s Scientific Manuscript database

Rust fungi infect a wide range of plant species making them of particular interest to plant pathologists. In order to study the interactions between these important pathogenic fungi and their host plants it is useful to be able to differentiate fungal tissue from plant tissue. This can be accomplish...

On the genetic control of planar growth during tissue morphogenesis in plants.

PubMed

Enugutti, Balaji; Kirchhelle, Charlotte; Schneitz, Kay

2013-06-01

Tissue morphogenesis requires extensive intercellular communication. Plant organs are composites of distinct radial cell layers. A typical layer, such as the epidermis, is propagated by stereotypic anticlinal cell divisions. It is presently unclear what mechanisms coordinate cell divisions relative to the plane of a layer, resulting in planar growth and maintenance of the layer structure. Failure in the regulation of coordinated growth across a tissue may result in spatially restricted abnormal growth and the formation of a tumor-like protrusion. Therefore, one way to approach planar growth control is to look for genetic mutants that exhibit localized tumor-like outgrowths. Interestingly, plants appear to have evolved quite robust genetic mechanisms that govern these aspects of tissue morphogenesis. Here we provide a short summary of the current knowledge about the genetics of tumor formation in plants and relate it to the known control of coordinated cell behavior within a tissue layer. We further portray the integuments of Arabidopsis thaliana as an excellent model system to study the regulation of planar growth. The value of examining this process in integuments was established by the recent identification of the Arabidopsis AGC VIII kinase UNICORN as a novel growth suppressor involved in the regulation of planar growth and the inhibition of localized ectopic growth in integuments and other floral organs. An emerging insight is that misregulation of central determinants of adaxial-abaxial tissue polarity can lead to the formation of spatially restricted multicellular outgrowths in several tissues. Thus, there may exist a link between the mechanisms regulating adaxial-abaxial tissue polarity and planar growth in plants.

Enhanced cellulose orientation analysis in complex model plant tissues.

PubMed

Rüggeberg, Markus; Saxe, Friederike; Metzger, Till H; Sundberg, Björn; Fratzl, Peter; Burgert, Ingo

2013-09-01

The orientation distribution of cellulose microfibrils in the plant cell wall is a key parameter for understanding anisotropic plant growth and mechanical behavior. However, precisely visualizing cellulose orientation in the plant cell wall has ever been a challenge due to the small size of the cellulose microfibrils and the complex network of polymers in the plant cell wall. X-ray diffraction is one of the most frequently used methods for analyzing cellulose orientation in single cells and plant tissues, but the interpretation of the diffraction images is complex. Traditionally, circular or square cells and Gaussian orientation of the cellulose microfibrils have been assumed to elucidate cellulose orientation from the diffraction images. However, the complex tissue structures of common model plant systems such as Arabidopsis or aspen (Populus) require a more sophisticated approach. We present an evaluation procedure which takes into account the precise cell geometry and is able to deal with complex microfibril orientation distributions. The evaluation procedure reveals the entire orientation distribution of the cellulose microfibrils, reflecting different orientations within the multi-layered cell wall. By analyzing aspen wood and Arabidopsis stems we demonstrate the versatility of this method and show that simplifying assumptions on geometry and orientation distributions can lead to errors in the calculated microfibril orientation pattern. The simulation routine is intended to be used as a valuable tool for nanostructural analysis of plant cell walls and is freely available from the authors on request. Copyright © 2013 Elsevier Inc. All rights reserved.

Sample Preparation for Mass Spectrometry Imaging of Plant Tissues: A Review

PubMed Central

Dong, Yonghui; Li, Bin; Malitsky, Sergey; Rogachev, Ilana; Aharoni, Asaph; Kaftan, Filip; Svatoš, Aleš; Franceschi, Pietro

2016-01-01

Mass spectrometry imaging (MSI) is a mass spectrometry based molecular ion imaging technique. It provides the means for ascertaining the spatial distribution of a large variety of analytes directly on tissue sample surfaces without any labeling or staining agents. These advantages make it an attractive molecular histology tool in medical, pharmaceutical, and biological research. Likewise, MSI has started gaining popularity in plant sciences; yet, information regarding sample preparation methods for plant tissues is still limited. Sample preparation is a crucial step that is directly associated with the quality and authenticity of the imaging results, it therefore demands in-depth studies based on the characteristics of plant samples. In this review, a sample preparation pipeline is discussed in detail and illustrated through selected practical examples. In particular, special concerns regarding sample preparation for plant imaging are critically evaluated. Finally, the applications of MSI techniques in plants are reviewed according to different classes of plant metabolites. PMID:26904042

How-To-Do-It: Using Cauliflower to Demonstrate Plant Tissue Culture.

ERIC Educational Resources Information Center

Haldeman, Janice H.; Ellis, Jane P.

1988-01-01

Presents techniques used for disinfestation of plant material, preparation of equipment and media, and laboratory procedures for tissue culture using cauliflower. Details methods for preparing solutions and plant propagation by cloning. (CW)

The influence of gravity and wind on land plant evolution.

PubMed

Niklas, K J

1998-07-01

Aspects of the engineering theory treating the elastic stability of vertical stems and cantilevered leaves supporting their own weight and additional wind-induced forces (drag) are reviewed in light of biomechanical studies of living and fossil terrestrial plant species. The maximum height to which arborescent species can grow before their stems elastically buckle under their own weight is estimated by means of the Euler-Greenhill formula which states that the critical buckling height scales as the 1/3 power of plant tissue-stiffness normalized with respect to tissue bulk density and as the 2/3 power of stem diameter. Data drawn from living plants indicate that progressively taller plant species employ stiffer and lighter-weight plant tissues as the principal stiffening agent in their vertical stems. The elastic stability of plants subjected to high lateral wind-loadings is governed by the drag torque (the product of the drag force and the height above ground at which this force is applied), which cannot exceed the gravitational bending moment (the product of the weight of aerial organs and the lever arm measured at the base of the plant). Data from living plants indicate that the largest arborescent plant species rely on massive trunks and broad, horizontally expansive root crowns to resist drag torques. The drag on the canopies of these plants is also reduced by highly flexible stems and leaves composed of tissues that twist and bend more easily than tissues used to stiffen older, more proximal stems. A brief review of the fossil record suggests that modifications in stem, leaf, and root morphology and anatomy capable of simultaneously coping with self-weight and wind-induced drag forces evolved by Devonian times, suggesting that natural selection acting on the elastic stability of sporophytes occurred early in the history of terrestrial plants.

Aerial Photography Summary Record System

USGS Publications Warehouse

,

1998-01-01

The Aerial Photography Summary Record System (APSRS) describes aerial photography projects that meet specified criteria over a given geographic area of the United States and its territories. Aerial photographs are an important tool in cartography and a number of other professions. Land use planners, real estate developers, lawyers, environmental specialists, and many other professionals rely on detailed and timely aerial photographs. Until 1975, there was no systematic approach to locate an aerial photograph, or series of photographs, quickly and easily. In that year, the U.S. Geological Survey (USGS) inaugurated the APSRS, which has become a standard reference for users of aerial photographs.

27. AERIAL VIEW LOOKING EAST DOWN THE WEST ACCESS ROAD. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. AERIAL VIEW LOOKING EAST DOWN THE WEST ACCESS ROAD. THE FIRST LARGE PROTEST AT THE PLANT CAME IN 1978. IT WAS THE FIRST MAJOR PROTEST AT ANY DEPARTMENT OF ENERGY PLANT. IN RESPONSE TO CONTINUING ANTI- NUCLEAR PROTESTS, IN PARTICULAR A 1979 RALLY THAT DREW 10,000 PARTICIPANTS, ROCKWELL EMPLOYEES AT THE PLANT FORMED A GRASSROOT ORGANIZATION, CITIZENS FOR ENERGY AND FREEDOM, AND ORGANIZED A PRO-NUCLEAR RALLY, 'POWER TO THE PEOPLE,' THAT ATTRACTED 16,000 PEOPLE (5/4/78). - Rocky Flats Plant, Bounded by Indiana Street & Routes 93, 128 & 72, Golden, Jefferson County, CO

[Comparative study on alkaloids of tissue-culture seedling and wild plant of Dendrobium huoshanense ].

PubMed

Chen, Nai-dong; Gao, Feng; Lin, Xin; Jin, Hui

2014-06-01

To compare the composition and content of alkaloid of Dendrobium huoshanense tissue-culture seedling and wild plant. A comparative evaluation on the quality was carried out by HPLC and TLC methods including the composition and the content of alkaloids. Remarkable variation existed in the two kinds of Dendrobium huoshanense. For the tissue-culture plant, only two alkaloids were checked out by both HPLC and TLC while four alkaloids were observed in the wild plant. The alkaloid content of tissue-culture seedling and wild plant was(0. 29 ± 0. 11)%o and(0. 43 ± 0. 15) %o,respectively. Distinguished difference is observed in both composition and content of alkaloids from the annual shoots of different provenances of Dendrobium huoshanense. It suggested that the quality of tissue-culture seedling of Dendrobium huoshanense might be inconsistent with the wild plant. Furthermore, the established alkaloids-knock-out HPLC method would provide a new research tool on quality control of Chinese medicinal materials which contain unknown alkaloids.

11. Copy of early 20th century photograph, an aerial view, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Copy of early 20th century photograph, an aerial view, showing the plant from the south looking north. Photo owned by the Parker- Hannifin Corporation. - Cleveland-Chandler Motors Corporation, 300 East 131st Street, Cleveland, Cuyahoga County, OH

The Development and Flight Testing of an Aerially Deployed Unmanned Aerial System

NASA Astrophysics Data System (ADS)

Smith, Andrew

An investigation into the feasibility of aerial deployed unmanned aerial vehicles was completed. The investigation included the development and flight testing of multiple unmanned aerial systems to investigate the different components of potential aerial deployment missions. The project consisted of two main objectives; the first objective dealt with the development of an airframe capable of surviving aerial deployment from a rocket and then self assembling from its stowed configuration into its flight configuration. The second objective focused on the development of an autopilot capable of performing basic guidance, navigation, and control following aerial deployment. To accomplish these two objectives multiple airframes were developed to verify their completion experimentally. The first portion of the project, investigating the feasibility of surviving an aerial deployment, was completed using a fixed wing glider that following a successful deployment had 52 seconds of controlled flight. Before developing the autopilot in the second phase of the project, the glider was significantly upgraded to fix faults discovered in the glider flight testing and to enhance the system capabilities. Unfortunately to conform to outdoor flight restrictions imposed by the university and the Federal Aviation Administration it was required to switch airframes before flight testing of the new fixed wing platform could begin. As a result, an autopilot was developed for a quadrotor and verified experimentally completely indoors to remain within the limits of governing policies.

Is polyploidy necessary for tissue differentiation in higher plants. [Triticum, helianthus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Evans, L.S.; Hof, J.V.

1975-01-01

Measurements of relative DNA per nucleus of cells from various tissues show that cell differentiation can occur in the absence of polyploidy in higher plants. In Pisum polyploidy was present in roots, sepals, pods, pistils, and stamens but not in petals or leaves. In Triticum cells of leaves exhibited some polyploidy, but no polyploid cells were present in mature roots. No polyploid cells were found in any tissue of Helianthus examined (roots, cotyledons, stems, sepals, petals, pistils, and stamens). Therefore, as a general rule, polyploidy should not be considered essential in tissue or organ differentiation of higher plants. In Helianthusmore » polyploidy is unnecessary for the completion of the life cycle. (auth)« less

Aerial Photography: Use in Detecting Simulated Insect Defoliation in Corn

NASA Technical Reports Server (NTRS)

Chiang, H. C.; Latham, R.; Meyer, M. P.

1973-01-01

Artificial defoliation in corn was used to explore the usefulness of aerial photography in detecting crop insect infestations. Defoliation on the top of plants was easily detected, while that on the base was less so. Aero infrared film with Wratten 89B filter gave the best results, and morning flights at the scale of 1:15840 are recommended. Row direction, plant growth stage, and time elapse since defoliation were not important factors.

47. CAPE COD AIR STATION PAVE PAWS FACILITY AERIAL VIEW ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

47. CAPE COD AIR STATION PAVE PAWS FACILITY AERIAL VIEW OF "A" FACE (LEFT) WITH CLEANING SYSTEM INSTALLED (NOW REMOVED) AND "B" FACE (RIGHT) WITH CONSTRUCTION CRANE IN USE. - Cape Cod Air Station, Technical Facility-Scanner Building & Power Plant, Massachusetts Military Reservation, Sandwich, Barnstable County, MA

Aerial Identification of Individual Cotton Plants

USDA-ARS?s Scientific Manuscript database

Boll weevils can infest individual cotton plants as well as cotton fields. However, volunteer and regrowth plants may grow where seed has scattered along roadsides and waterways, and in fields following rotation from cotton production. Timely areawide detection of these potential host plants is cr...

Tropine Forming Tropinone Reductase Gene from Withania somnifera (Ashwagandha): Biochemical Characteristics of the Recombinant Enzyme and Novel Physiological Overtones of Tissue-Wide Gene Expression Patterns

PubMed Central

Kushwaha, Amit Kumar; Sangwan, Neelam Singh; Trivedi, Prabodh Kumar; Negi, Arvind Singh; Misra, Laxminarain; Sangwan, Rajender Singh

2013-01-01

Withania somnifera is one of the most reputed medicinal plants of Indian systems of medicine synthesizing diverse types of secondary metabolites such as withanolides, alkaloids, withanamides etc. Present study comprises cloning and E. coli over-expression of a tropinone reductase gene (WsTR-I) from W. somnifera, and elucidation of biochemical characteristics and physiological role of tropinone reductase enzyme in tropane alkaloid biosynthesis in aerial tissues of the plant. The recombinant enzyme was demonstrated to catalyze NADPH-dependent tropinone to tropine conversion step in tropane metabolism, through TLC, GC and GC-MS-MS analyses of the reaction product. The functionally active homodimeric ∼60 kDa enzyme catalyzed the reaction in reversible manner at optimum pH 6.7. Catalytic kinetics of the enzyme favoured its forward reaction (tropine formation). Comparative 3-D models of landscape of the enzyme active site contours and tropinone binding site were also developed. Tissue-wide and ontogenic stage-wise assessment of WsTR-I transcript levels revealed constitutive expression of the gene with relatively lower abundance in berries and young leaves. The tissue profiles of WsTR-I expression matched those of tropine levels. The data suggest that, in W. somnifera, aerial tissues as well possess tropane alkaloid biosynthetic competence. In vivo feeding of U-[14C]-sucrose to orphan shoot (twigs) and [14C]-chasing revealed substantial radiolabel incorporation in tropinone and tropine, confirming the de novo synthesizing ability of the aerial tissues. This inherent independent ability heralds a conceptual novelty in the backdrop of classical view that these tissues acquire the alkaloids through transportation from roots rather than synthesis. The TR-I gene expression was found to be up-regulated on exposure to signal molecules (methyl jasmonate and salicylic acid) and on mechanical injury. The enzyme's catalytic and structural properties as well as gene expression

2. AERIAL VIEW OF MINUTEMAN SILOS. Low oblique aerial view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. AERIAL VIEW OF MINUTEMAN SILOS. Low oblique aerial view (original in color) of the two launch silos, covered. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Missile Silo Type, Test Area 1-100, northeast end of Test Area 1-100 Road, Boron, Kern County, CA

The Development of an Open Hardware and Software System Onboard Unmanned Aerial Vehicles to Monitor Concentrated Solar Power Plants

PubMed Central

Mesas-Carrascosa, Francisco Javier; Verdú Santano, Daniel; Pérez Porras, Fernando; Meroño-Larriva, José Emilio; García-Ferrer, Alfonso

2017-01-01

Concentrated solar power (CSP) plants are increasingly gaining interest as a source of renewable energy. These plants face several technical problems and the inspection of components such as absorber tubes in parabolic trough concentrators (PTC), which are widely deployed, is necessary to guarantee plant efficiency. This article presents a system for real-time industrial inspection of CSP plants using low-cost, open-source components in conjunction with a thermographic sensor and an unmanned aerial vehicle (UAV). The system, available in open-source hardware and software, is designed to be employed independently of the type of device used for inspection (laptop, smartphone, tablet or smartglasses) and its operating system. Several UAV flight missions were programmed as follows: flight altitudes at 20, 40, 60, 80, 100 and 120 m above ground level; and three cruising speeds: 5, 7 and 10 m/s. These settings were chosen and analyzed in order to optimize inspection time. The results indicate that it is possible to perform inspections by an UAV in real time at CSP plants as a means of detecting anomalous absorber tubes and improving the effectiveness of methodologies currently being utilized. Moreover, aside from thermographic sensors, this contribution can be applied to other sensors and can be used in a broad range of applications where real-time georeferenced data visualization is necessary. PMID:28594353

The Development of an Open Hardware and Software System Onboard Unmanned Aerial Vehicles to Monitor Concentrated Solar Power Plants.

PubMed

Mesas-Carrascosa, Francisco Javier; Verdú Santano, Daniel; Pérez Porras, Fernando; Meroño-Larriva, José Emilio; García-Ferrer, Alfonso

2017-06-08

Concentrated solar power (CSP) plants are increasingly gaining interest as a source of renewable energy. These plants face several technical problems and the inspection of components such as absorber tubes in parabolic trough concentrators (PTC), which are widely deployed, is necessary to guarantee plant efficiency. This article presents a system for real-time industrial inspection of CSP plants using low-cost, open-source components in conjunction with a thermographic sensor and an unmanned aerial vehicle (UAV). The system, available in open-source hardware and software, is designed to be employed independently of the type of device used for inspection (laptop, smartphone, tablet or smartglasses) and its operating system. Several UAV flight missions were programmed as follows: flight altitudes at 20, 40, 60, 80, 100 and 120 m above ground level; and three cruising speeds: 5, 7 and 10 m/s. These settings were chosen and analyzed in order to optimize inspection time. The results indicate that it is possible to perform inspections by an UAV in real time at CSP plants as a means of detecting anomalous absorber tubes and improving the effectiveness of methodologies currently being utilized. Moreover, aside from thermographic sensors, this contribution can be applied to other sensors and can be used in a broad range of applications where real-time georeferenced data visualization is necessary.

3. AERIAL VIEW, LOOKING SOUTH, OF BUILDING 371 BASEMENT UNDER ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. AERIAL VIEW, LOOKING SOUTH, OF BUILDING 371 BASEMENT UNDER CONSTRUCTION. THE BASEMENT HOUSES HEATING, VENTILATION, AND AIR CONDITIONING EQUIPMENT AND MECHANICAL UTILITIES, THE UPPER PART OF THE PLUTONIUM STORAGE VAULT AND MAINTENANCE BAY, AND SMALL PLUTONIUM PROCESSING AREAS. THE BASEMENT LEVEL IS DIVIDED INTO NEARLY EQUAL NORTH AND SOUTH PARTS BY THE UPPER PORTION OF THE PLUTONIUM STORAGE VAULT. (10/7/74) - Rocky Flats Plant, Plutonium Recovery Facility, Northwest portion of Rocky Flats Plant, Golden, Jefferson County, CO

Determination of Arsenical Herbicide Residues in Plant Tissues

Treesearch

R.M. Sachs; J.L. Michael; F.B. Anastasia; W.A. Wells

1971-01-01

Paper chromatographic separation of hydroxydimethylarsine oxide (cacodylic acid), monosodium methanearsonate (MSMA), sodium arsenate, and sodium arsenite was achieved with the aid of four solvent systems. Aqueous extracts of plant tissues removed essentially all the arscnicals applied, but mechanoiic fractionation was required before the extracts could be analyzed by...

Lactococcus lactis Metabolism and Gene Expression during Growth on Plant Tissues

PubMed Central

Golomb, Benjamin L.

2014-01-01

Lactic acid bacteria have been isolated from living, harvested, and fermented plant materials; however, the adaptations these bacteria possess for growth on plant tissues are largely unknown. In this study, we investigated plant habitat-specific traits of Lactococcus lactis during growth in an Arabidopsis thaliana leaf tissue lysate (ATL). L. lactis KF147, a strain originally isolated from plants, exhibited a higher growth rate and reached 7.9-fold-greater cell densities during growth in ATL than the dairy-associated strain L. lactis IL1403. Transcriptome profiling (RNA-seq) of KF147 identified 853 induced and 264 repressed genes during growth in ATL compared to that in GM17 laboratory culture medium. Genes induced in ATL included those involved in the arginine deiminase pathway and a total of 140 carbohydrate transport and metabolism genes, many of which are involved in xylose, arabinose, cellobiose, and hemicellulose metabolism. The induction of those genes corresponded with L. lactis KF147 nutrient consumption and production of metabolic end products in ATL as measured by gas chromatography-time of flight mass spectrometry (GC-TOF/MS) untargeted metabolomic profiling. To assess the importance of specific plant-inducible genes for L. lactis growth in ATL, xylose metabolism was targeted for gene knockout mutagenesis. Wild-type L. lactis strain KF147 but not an xylA deletion mutant was able to grow using xylose as the sole carbon source. However, both strains grew to similarly high levels in ATL, indicating redundancy in L. lactis carbohydrate metabolism on plant tissues. These findings show that certain strains of L. lactis are well adapted for growth on plants and possess specific traits relevant for plant-based food, fuel, and feed fermentations. PMID:25384484

New insights into Fe localization in plant tissues

PubMed Central

Roschzttardtz, Hannetz; Conéjéro, Geneviève; Divol, Fanchon; Alcon, Carine; Verdeil, Jean-Luc; Curie, Catherine; Mari, Stéphane

2013-01-01

Deciphering cellular iron (Fe) homeostasis requires having access to both quantitative and qualitative information on the subcellular pools of Fe in tissues and their dynamics within the cells. We have taken advantage of the Perls/DAB Fe staining procedure to perform a systematic analysis of Fe distribution in roots, leaves and reproductive organs of the model plant Arabidopsis thaliana, using wild-type and mutant genotypes affected in iron transport and storage. Roots of soil-grown plants accumulate iron in the apoplast of the central cylinder, a pattern that is strongly intensified when the citrate effluxer FRD3 is not functional, thus stressing the importance of citrate in the apoplastic movement of Fe. In leaves, Fe level is low and only detected in and around vascular tissues. In contrast, Fe staining in leaves of iron-treated plants extends in the surrounding mesophyll cells where Fe deposits, likely corresponding to Fe-ferritin complexes, accumulate in the chloroplasts. The loss of ferritins in the fer1,3,4 triple mutant provoked a massive accumulation of Fe in the apoplastic space, suggesting that in the absence of iron buffering in the chloroplast, cells activate iron efflux and/or repress iron influx to limit the amount of iron in the cell. In flowers, Perls/DAB staining has revealed a major sink for Fe in the anthers. In particular, developing pollen grains accumulate detectable amounts of Fe in small-size intracellular bodies that aggregate around the vegetative nucleus at the binuclear stage and that were identified as amyloplasts. In conclusion, using the Perls/DAB procedure combined to selected mutant genotypes, this study has established a reliable atlas of Fe distribution in the main Arabidopsis organs, proving and refining long-assumed intracellular locations and uncovering new ones. This “iron map” of Arabidopsis will serve as a basis for future studies of possible actors of iron movement in plant tissues and cell compartments. PMID:24046774

Using Peat Pellets in Liquid Media to Root Sunflower Tissue Culture Plants

USDA-ARS?s Scientific Manuscript database

Traditional plant breeding is often limited by the genetic diversity within a species. The use of biotechnology allows introducing into a plant, specific traits that come from the same or another plant species. In this paper, we focus on tissue culture of sunflower (Helianthus annus L., Asteraceae...

Aerial Explorers and Robotic Ecosystems

NASA Technical Reports Server (NTRS)

Young, Larry A.; Pisanich, Greg

2004-01-01

A unique bio-inspired approach to autonomous aerial vehicle, a.k.a. aerial explorer technology is discussed. The work is focused on defining and studying aerial explorer mission concepts, both as an individual robotic system and as a member of a small robotic "ecosystem." Members of this robotic ecosystem include the aerial explorer, air-deployed sensors and robotic symbiotes, and other assets such as rovers, landers, and orbiters.

Plasmid-dependent attachment of Agrobacterium tumefaciens to plant tissue culture cells.

PubMed

Matthysse, A G; Wyman, P M; Holmes, K V

1978-11-01

Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. Five Ti-plasmid-containing virulent Agrobacterium strains showed greater attachment to tobacco cells than did five avirulent strains. Light and scanning electron microscopic observations confirmed that virulent strains showed little attachment. Bacterial attachment was blocked by prior incubation of the plant cells with lipopolysaccharide extracted from A. tumefaciens, but not from A. radiobacter, suggesting that bacterial lipopolysaccharide is one of the components involved in the attachment process. At least one other bacterial product may be required for attachment in tissue culture because the virulent A. tumefaciens NT1, which lacks the Ti plasmid, does not itself attach to tobacco cells, but its lipopolysaccharide does inhibit the attachment of virulent strains.

Plasmid-dependent attachment of Agrobacterium tumefaciens to plant tissue culture cells.

PubMed Central

Matthysse, A G; Wyman, P M; Holmes, K V

1978-01-01

Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. Five Ti-plasmid-containing virulent Agrobacterium strains showed greater attachment to tobacco cells than did five avirulent strains. Light and scanning electron microscopic observations confirmed that virulent strains showed little attachment. Bacterial attachment was blocked by prior incubation of the plant cells with lipopolysaccharide extracted from A. tumefaciens, but not from A. radiobacter, suggesting that bacterial lipopolysaccharide is one of the components involved in the attachment process. At least one other bacterial product may be required for attachment in tissue culture because the virulent A. tumefaciens NT1, which lacks the Ti plasmid, does not itself attach to tobacco cells, but its lipopolysaccharide does inhibit the attachment of virulent strains. Images PMID:730370

Investigation of viability of plant tissue in the environmental scanning electron microscopy.

PubMed

Zheng, Tao; Waldron, K W; Donald, Athene M

2009-11-01

The advantages of environmental scanning electron microscopy (ESEM) make it a suitable technique for studying plant tissue in its native state. There have been few studies on the effects of ESEM environment and beam damage on the viability of plant tissue. A simple plant tissue, Allium cepa (onion) upper epidermal tissue was taken as the model for study. The change of moisture content of samples was studied at different relative humidities. Working with the electron beam on, viability tests were conducted for samples after exposure in the ESEM under different operating conditions to investigate the effect of electron beam dose on the viability of samples. The results suggested that without the electron beam, the ESEM chamber itself can prevent the loss of initial moisture if its relative humidity is maintained above 90%. With the electron beam on, the viability of Allium cepa (onion) cells depends both on the beam accelerating voltage and the electron dose/unit area hitting the sample. The dose can be controlled by several of the ESEM instrumental parameters. The detailed process of beam damage on cuticle-down and cuticle-up samples was investigated and compared. The results indicate that cuticular adhesion to the cell wall is relatively weak, but highly resistant to electron beam damage. Systematic study on the effect of ESEM operation parameters has been done. Results qualitatively support the intuitive expectations, but demonstrate quantitatively that Allium cepa epidermal cells are able to be kept in a hydrated and viable state under relevant operation condition inside ESEM, providing a basis for further in situ experiments on plant tissues.

A fully automatable enzymatic method for DNA extraction from plant tissues

PubMed Central

Manen, Jean-François; Sinitsyna, Olga; Aeschbach, Lorène; Markov, Alexander V; Sinitsyn, Arkady

2005-01-01

Background DNA extraction from plant tissues, unlike DNA isolation from mammalian tissues, remains difficult due to the presence of a rigid cell wall around the plant cells. Currently used methods inevitably require a laborious mechanical grinding step, necessary to disrupt the cell wall for the release of DNA. Results Using a cocktail of different carbohydrases, a method was developed that enables a complete digestion of the plant cell walls and subsequent DNA release. Optimized conditions for the digestion reaction minimize DNA shearing and digestion, and maximize DNA release from the plant cell. The method gave good results in 125 of the 156 tested species. Conclusion In combination with conventional DNA isolation techniques, the new enzymatic method allows to obtain high-yield, high-molecular weight DNA, which can be used for many applications, including genome characterization by AFLP, RAPD and SSR. Automation of the protocol (from leaf disks to DNA) is possible with existing workstations. PMID:16269076

Plant cell tissue culture: A potential source of chemicals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scott, C.D.; Dougall, D.K.

1987-08-01

Higher plants produce many industrially important products. Among these are drugs and medicinal chemicals, essential oils and flavors, vegetable oils and fats, fine and specialty chemicals, and even some commodity chemicals. Although, currently, whole-plant extraction is the primary means of harvesting these materials, the advent of plant cell tissue culture could be a much more effective method of producing many types of phytochemicals. The use of immobilized plant cells in an advanced bioreactor configuration with excretion of the product into the reactor medium may represent the most straightforward way of commercializing such techniques for lower-value chemicals. Important research and developmentmore » opportunities in this area include screening for plant cultures for nonmedical, lower-value chemicals; understanding and controlling plant cell physiology and biochemistry; optimizing effective immobilization methods; developing more efficient bioreactor concepts; and perfecting product extraction and purification techniques. 62 refs., 2 figs.« less

[Diversity and tissue distribution of fungal endophytes in Alpinia officinarum: an important south-China medicinal plant].

PubMed

Zhou, Ren-Chao; Huang, Juan; Li, Ze-En; Li, Shu-Bin

2014-08-01

In the present study, terminal-restriction fragment length polymorphism (T-RFLP) technique was applied to assess the diversity and tissue distribution of the fungal endophyte communities of Alpinia officinarum collected from Longtang town in Xuwen county, Guangdong province, China, at which the pharmacological effect of the medicine plant is traditional considered to be the significantly higher than that in any other growth areas in China. A total of 28 distinct Terminal-Restriction Fragment (T-RFs) were detected with HhaI Mono-digestion targeted amplified fungal nuclear ribosomal internal transcribed spacer region sequences (rDNA ITS) from the root, rhizome, stem, and leaf internal tissues of A. officinarum plant, indicating that at least 28 distinct fungal species were able to colonize the internal tissue of the host plant. The rDNA ITS-T-RFLP profiles obtained from different tissues of the host plant were obvious distinct. And the numbers of total T-RFs, and the dominant T-RFs detected from various tissues were significantly different. Based on the obtained T-RFLP profiles, Shannon's diversity index and the Shannon's evenness index were calculated, which were significantly different among tissues (P < 0.05). Furthermore, two types of active chemicals, total volatile oils by water vapor distillation method and galangin by methanol extraction-HPLC method, were examined in the each tissue of the tested plant. Both of tested components were detected in all of the four tissues of the medicine plant with varying contents. And the highest was in rhizome tissue. Correlation analysis revealed there were significant negative correlations between both of the tested active components contents and calculated Shannon's diversity index, as well as the Shannon's evenness index of the fungal endophyte communities of the host plant (P = 0, Pearson correlation coefficient ≤ -0.962), and significant positive correlations between both of the tested active components contents and

Aerial photo SBVC1962". Photo no. 360. Low oblique aerial view ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Aerial photo -SBVC-1962". Photo no. 360. Low oblique aerial view of the campus, looking southeast. Stamped on the rear: "Ron Wilhite, Sun-Telegram photo, file, 10/22/62/ - San Bernardino Valley College, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

The role of activated charcoal in plant tissue culture.

PubMed

Thomas, T Dennis

2008-01-01

Activated charcoal has a very fine network of pores with large inner surface area on which many substances can be adsorbed. Activated charcoal is often used in tissue culture to improve cell growth and development. It plays a critical role in micropropagation, orchid seed germination, somatic embryogenesis, anther culture, synthetic seed production, protoplast culture, rooting, stem elongation, bulb formation etc. The promotary effects of AC on morphogenesis may be mainly due to its irreversible adsorption of inhibitory compounds in the culture medium and substancially decreasing the toxic metabolites, phenolic exudation and brown exudate accumulation. In addition to this activated charcoal is involved in a number of stimulatory and inhibitory activities including the release of substances naturally present in AC which promote growth, alteration and darkening of culture media, and adsorption of vitamins, metal ions and plant growth regulators, including abscisic acid and gaseous ethylene. The effect of AC on growth regulator uptake is still unclear but some workers believe that AC may gradually release certain adsorbed products, such as nutrients and growth regulators which become available to plants. This review focuses on the various roles of activated charcoal in plant tissue culture and the recent developments in this area.

Improved method for HPLC analysis of polyamines, agmatine and aromatic monoamines in plant tissue

NASA Technical Reports Server (NTRS)

Slocum, R. D.; Flores, H. E.; Galston, A. W.; Weinstein, L. H.

1989-01-01

The high performance liquid chromatographic (HPLC) method of Flores and Galston (1982 Plant Physiol 69: 701) for the separation and quantitation of benzoylated polyamines in plant tissues has been widely adopted by other workers. However, due to previously unrecognized problems associated with the derivatization of agmatine, this important intermediate in plant polyamine metabolism cannot be quantitated using this method. Also, two polyamines, putrescine and diaminopropane, also are not well resolved using this method. A simple modification of the original HPLC procedure greatly improves the separation and quantitation of these amines, and further allows the simulation analysis of phenethylamine and tyramine, which are major monoamine constituents of tobacco and other plant tissues. We have used this modified HPLC method to characterize amine titers in suspension cultured carrot (Daucas carota L.) cells and tobacco (Nicotiana tabacum L.) leaf tissues.

RDX in Plant Tissue: Leading to Humification in Surface Soils

DTIC Science & Technology

2013-01-01

enzymatic activities could accel- erate the degradation of RDX once taken up from an aqueous solution . Plant tissue with higher chlorophyll content was found...whereas GSH inhibited it. Photo-induced degrada- tion of TNT occurs at approximately the same rate in extract-based solution . The results indi- cate...RDX-water solution taken up by each plant species................................. 10 Figure 3. Pigmentation patterns of all coleus used

Soluble inorganic tissue phosphorus and calcicole-calcifuge behaviour of plants.

PubMed

Zohlen, Angelika; Tyler, Germund

2004-09-01

Natural and semi-natural, non-fertilized calcareous soils are consistently low in soluble and easily exchangeable phosphate. An over-utilization, or possibly an immobilization, of inorganic P in the tissues of calcifuge plants may take place, if such plants are forced to grow on a calcareous soil, though this has not been experimentally demonstrated. The objectives of this study are, therefore, to elucidate if calcifuge plants, when forced to develop on a calcareous soil, not only have lower total P (Ptot) concentrations in their leaves than calcicole plants grown on such soil, but also a lower proportion of Ptot as water-soluble, inorganic phosphate. Such differences may be of importance in understanding the calcicole-calcifuge behaviour of plants. Plants of five calcicole and five calcifuge herbs and three calcicole and three calcifuge grasses were cultivated in a glasshouse on a moderately acid Cambisol and a calcareous Rendzic Leptosol using seeds of wild populations from southern Sweden. The calcifuges were: Corynephorus canescens, Deschampsia flexuosa, Holcus mollis, Digitalis purpurea, Lychnis viscaria, Rumex acetosella, Scleranthus annuus and Silene rupestris. The calcicoles were: Melica ciliata, Phleum phleoides, Sesleria caerulea, Arabis hirsuta, Sanguisorba minor, Scabiosa columbaria, Silene uniflora ssp. petraea and Veronica spicata. At harvest, calcifuges had much lower leaf tissue concentrations of Ptot and Pi than calcicoles when grown on the calcareous soil, and biomass production of the calcifuges was poor on this soil. Moreover, the calcifuge herbs had, on average, a lower proportion of their Ptot as Pi than had the calcicole herbs. The calcifuge herbs were also unable to avoid excessive uptake of Ca from the calcareous soil. The calcifuge grasses maintained a similar proportion of Ptot as Pi as the calcicole grasses, but their growth was still poor on the calcareous soil. On calcareous soil, very little Pi in the tissues of calcifuge herbs is, at

Final Environmental Assessment for Aerial Spraying for Invasive Species Control MacDill AFB, Florida

DTIC Science & Technology

2007-09-01

bark tree (Melaleuca quinquinervia), Australian pine (Casuarina equisetifolia), Japanese mimosa (Albizia julibrissin) and, cogan grass (Imperata...inaccessible areas of the base. 2.1 DETAILED DESCRIPTION OF THE PROPOSED ACTION Invasive plant species such as Brazilian pepper, melaleuca, and mimosa are...principle invasive plant species proposed for treatment through the aerial application of herbicides include Brazilian pepper, melaleuca, and mimosa

Prevention of pink-pigmented methylotrophic bacteria (Methylohacterium mesophilicum) contamination of plant tissue cultures.

PubMed

Chanprame, S; Todd, J J; Widholm, J M

1996-12-01

Pink-pigmented facultative methylotrophic bacteria (PPFMs) have been found on the surfaces of leaves of most plants tested. We found PPFMs on the leaf surfaces of all 40 plants (38 species) tested and on soybean pods by pressing onto AMS medium with methanol as the sole carbon source. The abundance ranged from 0.5 colony forming unit (cfu) /cm(2) to 69.4 cfu/cm(2) on the leaf surfaces. PPFMs were found in homogenized leaf tissues of only 4 of the species after surface disinfestation with 1.05% sodium hypochlorite and were rarely found in cultures initiated from surface disinfested Datura innoxia leaves or inside surface disinfested soybean pods. Of 20 antibiotics tested for PPFM growth inhibition, rifampicin was the most effective and of seven others which also inhibited PPFM growth, cefotaxime should be the most useful due to the expected low plant cell toxicity. These antibiotics could be used in concert with common surface sterilization procedures to prevent the introduction or to eliminate PPFM bacteria in tissue cultures. Thus, while PPFMs are present on the surfaces of most plant tissues, surface disinfestation alone can effectively remove them so that uncontaminated tissue cultures can be initiated in most cases.

24. Duplicate negative of an historic negative. 'AERIAL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. Duplicate negative of an historic negative. 'AERIAL VIEW OF AREA 'B' HOLSTON ORDNANCE WORKS.' 1944. #OCMH 4-12.2ASAV3 in Super Explosives Program RDX and Its Composition A, B, & C, Record Group No. 319, National Archives, Washington, D.C. - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

Nanoparticles as smart treatment-delivery systems in plants: assessment of different techniques of microscopy for their visualization in plant tissues.

PubMed

González-Melendi, P; Fernández-Pacheco, R; Coronado, M J; Corredor, E; Testillano, P S; Risueño, M C; Marquina, C; Ibarra, M R; Rubiales, D; Pérez-de-Luque, A

2008-01-01

The great potential of using nanodevices as delivery systems to specific targets in living organisms was first explored for medical uses. In plants, the same principles can be applied for a broad range of uses, in particular to tackle infections. Nanoparticles tagged to agrochemicals or other substances could reduce the damage to other plant tissues and the amount of chemicals released into the environment. To explore the benefits of applying nanotechnology to agriculture, the first stage is to work out the correct penetration and transport of the nanoparticles into plants. This research is aimed (a) to put forward a number of tools for the detection and analysis of core-shell magnetic nanoparticles introduced into plants and (b) to assess the use of such magnetic nanoparticles for their concentration in selected plant tissues by magnetic field gradients. Cucurbita pepo plants were cultivated in vitro and treated with carbon-coated Fe nanoparticles. Different microscopy techniques were used for the detection and analysis of these magnetic nanoparticles, ranging from conventional light microscopy to confocal and electron microscopy. Penetration and translocation of magnetic nanoparticles in whole living plants and into plant cells were determined. The magnetic character allowed nanoparticles to be positioned in the desired plant tissue by applying a magnetic field gradient there; also the graphitic shell made good visualization possible using different microscopy techniques. The results open a wide range of possibilities for using magnetic nanoparticles in general plant research and agronomy. The nanoparticles can be charged with different substances, introduced within the plants and, if necessary, concentrated into localized areas by using magnets. Also simple or more complex microscopical techniques can be used in localization studies.

AERIAL MEASURING SYSTEM IN JAPAN

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lyons, Craig; Colton, David

2012-01-01

The U.S. Department of Energy National Nuclear Security Agency’s Aerial Measuring System deployed personnel and equipment to partner with the U.S. Air Force in Japan to conduct multiple aerial radiological surveys. These were the first and most comprehensive sources of actionable information for U.S. interests in Japan and provided early confirmation to the government of Japan as to the extent of the release from the Fukushima Daiichi Nuclear Power Generation Station. Many challenges were overcome quickly during the first 48 hours; including installation and operation of Aerial Measuring System equipment on multiple U.S. Air Force Japan aircraft, flying over difficultmore » terrain, and flying with talented pilots who were unfamiliar with the Aerial Measuring System flight patterns. These all combined to make for a dynamic and non-textbook situation. In addition, the data challenges of the multiple and on-going releases, and integration with the Japanese government to provide valid aerial radiological survey products that both military and civilian customers could use to make informed decisions, was extremely complicated. The Aerial Measuring System Fukushima response provided insight in addressing these challenges and gave way to an opportunity for the expansion of the Aerial Measuring System’s mission beyond the borders of the US.« less

Aerial Survey Results for 131I Deposition on the Ground after the Fukushima Daiichi Nuclear Power Plant Accident

DOE Office of Scientific and Technical Information (OSTI.GOV)

Torii, Tatsuo; Sugita, Takeshi; Okada, Colin E.

In March 2011 the second largest accidental release of radioactivity in history occurred at the Fukushima Daiichi nuclear power plant following a magnitude 9.0 earthquake and subsequent tsunami. Teams from the U.S. Department of Energy, National Nuclear Security Administration Office of Emergency Response performed aerial surveys to provide initial maps of the dispersal of radioactive material in Japan. The initial results from the surveys did not report the concentration of 131I. This work reports on analyses performed on the initial survey data by a joint Japan-US collaboration to determine 131I ground concentration. This information is potentially useful in reconstruction ofmore » the inhalation and external exposure doses from this short-lived radionuclide. The deposited concentration of 134Cs is also reported.« less

Chemical constituents from aerial part of Curcuma wenyujin.

PubMed

Tao, Zheng-Ming; Li, Ying-Yu; Ji, Ping; Wang, Yu-Bo; Qin, Guo-Wei

2007-12-01

To investigate the chemical constituents from aerial part of Curcuma wenyujin. Compounds were isolated by repeated column chromatography on silica gel. Their structures were elucidated on the basis of spectral analysis and comparison with literature data. Six compounds were isolated and identified as codonolactone (1), voleneol (2), octacosanoic acid (3), beta-sitosterol (4), mangdesisterol (5), and daucosterol (6). Compounds 1, 2, and 5 were isolated from the plant for the first time.

Plant Tissues in 3D via X-Ray Tomography: Simple Contrasting Methods Allow High Resolution Imaging

PubMed Central

Staedler, Yannick M.; Masson, David; Schönenberger, Jürg

2013-01-01

Computed tomography remains strongly underused in plant sciences despite its high potential in delivering detailed 3D phenotypical information because of the low X-ray absorption of most plant tissues. Existing protocols to study soft tissues display poor performance, especially when compared to those used on animals. More efficient protocols to study plant material are therefore needed. Flowers of Arabidopsis thaliana and Marcgravia caudata were immersed in a selection of contrasting agents used to treat samples for transmission electron microscopy. Grayscale values for floral tissues and background were measured as a function of time. Contrast was quantified via a contrast index. The thick buds of Marcgravia were scanned to determine which contrasting agents best penetrate thick tissues. The highest contrast increase with cytoplasm-rich tissues was obtained with phosphotungstate, whereas osmium tetroxide and bismuth tatrate displayed the highest contrast increase with vacuolated tissues. Phosphotungstate also displayed the best sample penetration. Furthermore, infiltration with phosphotungstate allowed imaging of all plants parts at a high resolution of 3 µm, which approaches the maximum resolution of our equipment: 1.5 µm. The high affinity of phosphotungstate for vasculature, cytoplasm-rich tissue, and pollen causes these tissues to absorb more X-rays than the surrounding tissues, which, in turn, makes these tissues appear brighter on the scan data. Tissues with different brightness can then be virtually dissected from each other by selecting the bracket of grayscale to be visualized. Promising directions for the future include in silico phenotyping and developmental studies of plant inner parts (e.g., ovules, vasculature, pollen, and cell nuclei) via virtual dissection as well as correlations of quantitative phenotypes with omics datasets. Therefore, this work represents a crucial improvement of previous methods, allowing new directions of research to be

The mechanics behind plant development.

PubMed

Hamant, Olivier; Traas, Jan

2010-01-01

Morphogenesis in living organisms relies on the integration of both biochemical and mechanical signals. During the last decade, attention has been mainly focused on the role of biochemical signals in patterning and morphogenesis, leaving the contribution of mechanics largely unexplored. Fortunately, the development of new tools and approaches has made it possible to re-examine these processes. In plants, shape is defined by two local variables: growth rate and growth direction. At the level of the cell, these variables depend on both the cell wall and turgor pressure. Multidisciplinary approaches have been used to understand how these cellular processes are integrated in the growing tissues. These include quantitative live imaging to measure growth rate and direction in tissues with cellular resolution. In parallel, stress patterns have been artificially modified and their impact on strain and cell behavior been analysed. Importantly, computational models based on analogies with continuum mechanics systems have been useful in interpreting the results. In this review, we will discuss these issues focusing on the shoot apical meristem, a population of stem cells that is responsible for the initiation of the aerial organs of the plant.

[Study on the chemical constituents of aerial part of Ligusticum jeholense].

PubMed

Sun, Jia-ming; Zhang, Bo; Chang, Ren-long; Ye, Dou-dan; Zhang, Hui

2011-07-01

To study the chemical constituents of the aerial part of Ligusticum jeholense. The constituents were isolated by sillica gel column chromatography, Sephadex LH-20 column chromatography and their structures were elucidated by spectral analysis. Seven compounds were separated from the EtOH extracts. Their structures were identified as psoralen (1), beta-sitosterol (2), daucosterol (3), kaempferol-3-O-(2",4"-di-E-p-coumaroyl)-alpha-L-rhamnoside (4), kaempferol-3-O-beta-D-galactoside (5), quercetin-3-O-beta-D-galactoside (6), sucrose (7). Compounds 1, 4, 5 and 6 are isolated from the genus for the first time. Compounds 2, 3 and 7 are isolated from the aerial part of the plant for the first time.

Analysis of chemical components from plant tissue samples

NASA Technical Reports Server (NTRS)

Laseter, J. L.

1972-01-01

Information is given on the type and concentration of sterols, free fatty acids, and total fatty acids in plant tissue samples. All samples were analyzed by gas chromatography and then by gas chromatography-mass spectrometry combination. In each case the mass spectral data was accumulated as a computer printout and plot. Typical gas chromatograms are included as well as tables describing test results.

Bioactive alkaloids from the aerial parts of Houttuynia cordata.

PubMed

Ma, Qinge; Wei, Rongrui; Wang, Zhiqiang; Liu, Wenmin; Sang, Zhipei; Li, Yaping; Huang, Hongchun

2017-01-04

Houttuynia cordata is an important traditional Chinese medicine used in heat-clearing and detoxifying, swelling and discharging pus, promoting diuresis and relieving stranguria which recorded in Pharmacopoeia of the people's Republic of China (2015 Edition). H. cordata has been recorded in the book Bencaogangmu which was written by Shizhen Li for the treatment of pyretic toxicity, carbuncle swelling, haemorrhoids, and rectocele diseases. Phytochemical investigation of the aerial parts of H. cordata and evaluation of their PTP1B inhibitory activities and hepatoprotective activities. The dried aerial parts of H. cordata were fractionated by liquid-liquid extraction to obtain CHCl 3 , ethyl acetate, and n-butanolic fractions. The CHCl 3 fraction was confirmed active fraction by the bioactivity-guided investigation, which was isolated and purified by chromatographing over silica gel, Sephadex LH-20, MPLC, and preparative HPLC. The chemical structures of the purified compounds were identified by their spectroscopic data and references. Eight new compounds (1-8), together with fourteen known compounds (9-22) were isolated from the aerial parts of H. cordata. The known compounds (9-22) were obtained from this plant for the first time. Among them, some compounds exhibited moderate bioactivities. Compounds (1-8) were identified as new alkaloids, and the known alkaloids (9-22) were isolated from this plant for the first time. Compounds 1, 4, 14, and 19 showed significant PTP1B inhibitory activities with IC 50 values of 1.254, 2.016, 2.672, and 1.862µm, respectively. Compounds 1, 3, 6, 11, 17, and 20 (10µm) exhibited moderate hepatoprotective activities against D-galactosamine-induced WB-F344 cells damage. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Using Tissue Culture To Investigate Plant Cell Differentiation and Dedifferentiation.

ERIC Educational Resources Information Center

Bozzone, Donna M.

1997-01-01

Describes an experimental project that uses plant tissue culture techniques to examine cell differentiation in the carrot. Allows students to gain experience in some important techniques and to explore fundamental questions about cell differentiation. (DDR)

Zooming In on Plant Hormone Analysis: Tissue- and Cell-Specific Approaches.

PubMed

Novák, Ondřej; Napier, Richard; Ljung, Karin

2017-04-28

Plant hormones are a group of naturally occurring, low-abundance organic compounds that influence physiological processes in plants. Our knowledge of the distribution profiles of phytohormones in plant organs, tissues, and cells is still incomplete, but advances in mass spectrometry have enabled significant progress in tissue- and cell-type-specific analyses of phytohormones over the last decade. Mass spectrometry is able to simultaneously identify and quantify hormones and their related substances. Biosensors, on the other hand, offer continuous monitoring; can visualize local distributions and real-time quantification; and, in the case of genetically encoded biosensors, are noninvasive. Thus, biosensors offer additional, complementary technologies for determining temporal and spatial changes in phytohormone concentrations. In this review, we focus on recent advances in mass spectrometry-based quantification, describe monitoring systems based on biosensors, and discuss validations of the various methods before looking ahead at future developments for both approaches.

Anti-inflammatory Activity of Constituents Isolated from Aerial Part of Angelica acutiloba Kitagawa.

PubMed

Uto, Takuhiro; Tung, Nguyen Huu; Taniyama, Risa; Miyanowaki, Tosihide; Morinaga, Osamu; Shoyama, Yukihiro

2015-12-01

Recently, the resources of medicinal plants have been exhausting. The root of Angelica acutiloba is one of the most important ingredients in Japanese Kampo medicine for the treatment of gynecological diseases. In our search for alternative medicinal plant resources of the root of A. acutiloba, we found that its aerial part has the anti-inflammatory potency as well as the root. Phytochemical investigation of the aerial part resulted in the isolation of four compounds including a new dimeric phthalide, namely tokiaerialide (2), along with Z-ligustilide (1), falcarindiol (3), and bergaptol (4). Next, we investigated the in vitro anti-inflammatory activity of 1-4 in lipopolysaccharide-stimulated RAW264 macrophages. Among the isolated compounds, 1 exhibited the most potent inhibition against lipopolysaccharide-induced production of prostaglandin E2 , nitric oxide, and pro-inflammatory cytokines (interleukin-6 and tumor necrosis factor-α). Compounds 3 and 4 also inhibited all inflammatory mediators, but their inhibitory abilities were weaker than those of 1. Furthermore, 1, 3, and 4 strongly also induced heme oxygenase-1. These results suggest that 1, 3, and 4 potentially exert anti-inflammatory activity, and the aerial part of A. acutiloba may be considered to be a useful medicinal resource for inflammatory diseases. Copyright © 2015 John Wiley & Sons, Ltd.

Pollutant-induced cell death and reactive oxygen species accumulation in the aerial roots of Chinese banyan (Ficus microcarpa)

NASA Astrophysics Data System (ADS)

Liu, Nan; Cao, Ce; Sun, Zhongyu; Lin, Zhifang; Deng, Rufang

2016-11-01

Industrial pollutants induce the production of toxic reactive oxygen species (ROS) such as O2.-, H2O2, and ·OH in plants, but they have not been well quantified or localized in tissues and cells. This study evaluated the pollutant- (HSO3-, NH4NO3, Al3+, Zn2+, and Fe2+) induced toxic effects of ROS on the aerial roots of Chinese banyan (Ficus microcarpa). Root cell viability was greatly reduced by treatment with 20 mM NaHSO3, 20 mM NH4NO3, 0.2 mM AlCl3, 0.2 mM ZnSO4, or 0.2 mM FeSO4. Biochemical assay and histochemical localization showed that O2.- accumulated in roots in response to pollutants, except that the staining of O2.- under NaHSO3 treatment was not detective. Cytochemical localization further indicated that the generated O2.- was present mainly in the root cortex, and pith cells, especially in NH4NO3- and FeSO4-treated roots. The pollutants also caused greatly accumulated H2O2 and ·OH in aerial roots, which finally resulted in lipid peroxidation as indicated by increased malondialdehyde contents. We conclude that the F. microcarpa aerial roots are sensitive to pollutant-induced ROS and that the histochemical localization of O2.- via nitrotetrazolium blue chloride staining is not effective for detecting the effects of HSO3- treatment because of the treatment’s bleaching effect.

Unmanned Aerial Vehicle (UAV) data analysis for fertilization dose assessment

NASA Astrophysics Data System (ADS)

Kavvadias, Antonis; Psomiadis, Emmanouil; Chanioti, Maroulio; Tsitouras, Alexandros; Toulios, Leonidas; Dercas, Nicholas

2017-10-01

The growth rate monitoring of crops throughout their biological cycle is very important as it contributes to the achievement of a uniformly optimum production, a proper harvest planning, and reliable yield estimation. Fertilizer application often dramatically increases crop yields, but it is necessary to find out which is the ideal amount that has to be applied in the field. Remote sensing collects spatially dense information that may contribute to, or provide feedback about, fertilization management decisions. There is a potential goal to accurately predict the amount of fertilizer needed so as to attain an ideal crop yield without excessive use of fertilizers cause financial loss and negative environmental impacts. The comparison of the reflectance values at different wavelengths, utilizing suitable vegetation indices, is commonly used to determine plant vigor and growth. Unmanned Aerial Vehicles (UAVs) have several advantages; because they can be deployed quickly and repeatedly, they are flexible regarding flying height and timing of missions, and they can obtain very high-resolution imagery. In an experimental crop field in Eleftherio Larissa, Greece, different dose of pre-plant and in-season fertilization was applied in 27 plots. A total of 102 aerial photos in two flights were taken using an Unmanned Aerial Vehicle based on the scheduled fertilization. Α correlation of experimental fertilization with the change of vegetation indices values and with the increase of the vegetation cover rate during those days was made. The results of the analysis provide useful information regarding the vigor and crop growth rate performance of various doses of fertilization.

Nanoparticles as Smart Treatment-delivery Systems in Plants: Assessment of Different Techniques of Microscopy for their Visualization in Plant Tissues

PubMed Central

González-Melendi, P.; Fernández-Pacheco, R.; Coronado, M. J.; Corredor, E.; Testillano, P. S.; Risueño, M. C.; Marquina, C.; Ibarra, M. R.; Rubiales, D.; Pérez-de-Luque, A.

2008-01-01

Background and Aims The great potential of using nanodevices as delivery systems to specific targets in living organisms was first explored for medical uses. In plants, the same principles can be applied for a broad range of uses, in particular to tackle infections. Nanoparticles tagged to agrochemicals or other substances could reduce the damage to other plant tissues and the amount of chemicals released into the environment. To explore the benefits of applying nanotechnology to agriculture, the first stage is to work out the correct penetration and transport of the nanoparticles into plants. This research is aimed (a) to put forward a number of tools for the detection and analysis of core-shell magnetic nanoparticles introduced into plants and (b) to assess the use of such magnetic nanoparticles for their concentration in selected plant tissues by magnetic field gradients. Methods Cucurbita pepo plants were cultivated in vitro and treated with carbon-coated Fe nanoparticles. Different microscopy techniques were used for the detection and analysis of these magnetic nanoparticles, ranging from conventional light microscopy to confocal and electron microscopy. Key Results Penetration and translocation of magnetic nanoparticles in whole living plants and into plant cells were determined. The magnetic character allowed nanoparticles to be positioned in the desired plant tissue by applying a magnetic field gradient there; also the graphitic shell made good visualization possible using different microscopy techniques. Conclusions The results open a wide range of possibilities for using magnetic nanoparticles in general plant research and agronomy. The nanoparticles can be charged with different substances, introduced within the plants and, if necessary, concentrated into localized areas by using magnets. Also simple or more complex microscopical techniques can be used in localization studies. PMID:17998213

1. AERIAL VIEW OF SLC3 FROM THE NORTHEAST SHOWING THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. AERIAL VIEW OF SLC-3 FROM THE NORTHEAST SHOWING THE EAST (LEFT) AND WEST (RIGHT) LAUNCH PADS, AND CABLE TRAYS FROM SLC-3W. SEWAGE TREATMENT PLANT (BLDG. 769) AND STORAGE SHED (BLDG. 773) IN LEFT FOREGROUND. - Vandenberg Air Force Base, Space Launch Complex 3, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

Prediction of atmospheric δ 13CO 2 using fossil plant tissues

DOE Office of Scientific and Technical Information (OSTI.GOV)

A. Hope Jahren; Arens, Nan Crystal; Harbeson, Stephanie A.

2008-06-30

To summarize the content: we presented the results of laboratory experiments designed to quantify the relationship between plant tissue δ 13C and δ 13CO 2 values under varying environmental conditions, including differential pCO 2 ranging from 1 to 3 times today’s levels. As predicted, plants grown under elevated pCO2 showed increased average biomass compared to controls grown at the same temperature. Across a very large range in δ 13Ca (≈ 24 ‰) and pCO 2 (≈ 740 ppmv) we observed a consistent correlation between δ13Ca and δ 13Cp (p<0.001). We show an average isotopic depletion of -25.4 ‰ for above-groundmore » tissue and -23.2 ‰ for below-ground tissue of Raphanus sativus L. relative to the composition of the atmosphere under which it formed. For both above- and below-ground tissue, grown at both ~23 °C and ~29 °C, correlation was strong and significant (r2 ≥ 0.98, p<0.001); variation in pCO 2 level had little or no effect on this relationship.« less

Cyanide Metabolism in Relation to Ethylene Production in Plant Tissues 1

PubMed Central

Yip, Wing-Kin; Yang, Shang Fa

1988-01-01

HCN is the putative product of C-1 and amino moieties of 1-aminocyclopropane-1-carboxylic acid (ACC) during its conversion to ethylene. In apple (Malus sylvestrus Mill.) slices or auxin-treated mungbean (Vigna radiata L.) hypocotyls, which produced ethylene at high rates, the steady state concentration of HCN was found to be no higher than 0.2 micromolar, which was too low to inhibit respiration (reported Ki for HCN to inhibit respiration was 10-20 micromolar). However, these tissues became cyanogenic when treated with ACC, the precursor of ethylene, and with 2-aminoxyacetic acid, which inhibits β-cyanoalanine synthase, the main enzyme to detoxify HCN; the HCN levels in these tissues went up to 1.7 and 8.1 micromolar, respectively. Although ethylene production by avocado (Persea gratissima) and apple fruits increased several hundred-fold during ripening, β-cyanoalanine synthase activity increased only one- to two-fold. These findings support the notion that HCN is a co-product of ethylene biosynthesis and that the plant tissues possess ample capacity to detoxify HCN formed during ethylene biosynthesis so that the concentration of HCN in plant tissues is kept at a low level. PMID:16666329

SPRUCE Pretreatment Plant Tissue Analyses, 2009 through 2013

DOE Data Explorer

Phillips, J. R. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Brice, D. J. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Hanson, P. J. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Childs, J. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Iversen, C. M. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Norby, R. J. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A; Warren, J. M. [Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A

2009-12-01

This data set reports the results of elemental analyses of foliar and stem/woody twig plant tissues collected at the SPRUCE site in 2009, 2012, and 2013. Samples were obtained at various locations around the S1 Bog and from within the developing experimental treatment plots. These are pretreatment vegetation samples, collected prior to initiation of the SPRUCE experiment heating and elevated CO2 treatments.

Lead detection in living plant tissue using a new histochemical method

DOE Office of Scientific and Technical Information (OSTI.GOV)

Glater, R.A.B.; Hernandez, L. Jr.

1972-06-01

A quick, simple method for identifying and distinguishing lead from other heavy metals in living plants has been developed using sodium rhodizonate (C/sub 6/O/sub 6/Na/sub 2/) which forms a scarlet precipitate with lead at approximately pH 2.8. Hand sections of plant tissues are treated with rhodizonate reagent, buffered, and examined microscopically. Very little time and/or effect is required for this method. Those cells and tissues contaminated with lead turn scarlet - color intensity being directly related to concentration. Lead may be detected in quite low concentrations and, most importantly, may be observed in situ; its entry and movement through themore » plant can thus be followed. In an area of moderate traffic of Downey, California (Southeast Los Angeles), lead was found abundantly on leaves as well as on and in roots of garden-grown lettuce; origin of this lead is presumed to be from car exhausts.« less

Temperature-dependent growth of Botrytis cinerea isolates from potted plants.

PubMed

Martínez, J A; Gómez-Bellot, M J; Bañón, S

2009-01-01

Botrytis cinereo is a common aggressive saprophyte fungus which also invades injured plant tissues, causing Botrytis blight (Grey mould) in many ornamental plants, including potted flowering plants. Several B. cinerea isolates from potted plants (Pelargonium x hortorum, Lantana camara, Lonicera japonica, Hydrangea macrophylla, and Cyclamen persicum) affected by Botrytis blight in the south of Spain were studied and identified by PCR. The isolates showed phenotypic differences between them, as previously reported by the authors. In this work we demonstrate that these isolates show different temperature-dependent growth phenomena, expressed as mycelial growth rates, conidiation (measured as the number of conidia per colony and time of appearance), mass of both aerial and submerged mycelia, and sclerotia production. Growth rates were assessed from differences in colony area and mass of both aerial and submerged mycelium growing in potato dextrose agar culture medium (PDA). Three temperatures were used to measure these variables (6, 16, and 26 degrees C) and to establish the differences among isolates by modelling the effects of temperature on the growth variables. B. cinerea showed a high degree of phenotypic variability and differences in its growth kinetics, depending on temperature and isolate in question. The isolate from P. x hortorum showed the greatest conidiation although this process did not depend on the temperatures assayed. The growth rate of the isolates from P. x hortorum was the highest. The growth rates in all the isolates were determined and the growth kinetics could be fitted to a typical equation of fungi growing on solid culture medium. The isolate from P. x hortorum was the most vigorous, while the least vigorous was the isolate from L. japonica. A relationship between mycelial growth rate, conidiation and aerial mycelium could be established. A temperature of 26 degrees C accelerated sclerotia production, but only in the isolate from C. persicum

Induction of kranz anatomy and C4-like biochemical characteristics in a submerged amphibious plant by abscisic acid

PubMed Central

Ueno, O

1998-01-01

The amphibious leafless sedge Eleocharis vivipara develops C4-like traits as well as Kranz anatomy under terrestrial conditions, but it develops C3-like traits without Kranz anatomy under submerged conditions. When submerged plants are exposed to aerial conditions, they rapidly produce new photosynthetic tissues with C4-like traits. In this study, experiments were performed to determine whether abscisic acid (ABA), a plant stress hormone, could induce the formation of photosynthetic tissues with Kranz anatomy and C4-like biochemical traits under water in the submerged form. When the submerged plants were grown in water containing 5 &mgr;M ABA, they developed new photosynthetic tissues with Kranz anatomy, forming well-developed Kranz (bundle sheath) cells that contained many organelles. The ABA-induced tissues accumulated large amounts of phosphoenolpyruvate carboxylase, pyruvate orthophosphate dikinase, and NAD-malic enzyme at the appropriate cellular sites. The tissues had 3.4 to 3.8 times more C4 enzyme activity than did tissues of the untreated submerged plants. Carbon-14 pulse and carbon-12 chase experiments revealed that the ABA-induced tissues fixed higher amounts of carbon-14 into C4 compounds and lower amounts of carbon-14 into C3 compounds as initial products than did the submerged plants and that they exhibited a C4-like pattern of carbon fixation under aqueous conditions of low carbon, indicating enhanced C4 capacity in the tissues. This report provides an example of the hormonal control of the differentiation of the structural and functional traits required for the C4 pathway. PMID:9548983

Use of aerial photography to inventory aquatic vegetation

USGS Publications Warehouse

Schloesser, Donald W.; Brown, Charles L.; Manny, Bruce A.

1988-01-01

This study demonstrates the feasibility of using low-altitude aerial photography to inventory submersed macrophytes in the connecting channels of the Great Lakes. For this purpose, we obtained aerial color transparencies and collateral ground truth information about submersed vegetation at 160 stations within four study sites in the St. Clair and Detroit rivers, September 17 to October 4, 1984. Photographs were interpreted by five test subjects to determine with what accuracy they could detect beds of submersed macrophytes, and the precision of delineating the extent of such vegetation beds. The interpreters correctly determined the presence or absence of vegetation 80% of the time (range 73-86%). Differences between individuals were statistically significant. Determination of the presence or absence of macrophytes depended partly on their relative abundance and water clarity. Analysis of one photograph from each of the four study sites revealed that photointerpreters delineated between 35 and 75 ha of river bottom covered by vegetation. This wide range indicates that individuals should be tested to assess their relative capability and be trained before they are employed to delineate plant beds in large-scale inventories. Within limits, low-altitude aerial photography, combined with collateral ground truth information, can be used to determine the presence or absence and delineate the extent of submersed macrophytes in connecting channels of the Great Lakes.

Selection of plants for phytoremediation of barium-polluted flooded soils.

PubMed

Ribeiro, Paulo Roberto Cleyton de Castro; Viana, Douglas Gomes; Pires, Fábio Ribeiro; Egreja Filho, Fernando Barboza; Bonomo, Robson; Cargnelutti Filho, Alberto; Martins, Luiz Fernando; Cruz, Leila Beatriz Silva; Nascimento, Mauro César Pinto

2018-05-10

The use of barite (BaSO4) in drilling fluids for oil and gas activities makes barium a potential contaminant in case of spills onto flooded soils, where low redox conditions may increase barium sulfate solubility. In order to select plants able to remove barium in such scenarios, the following species were evaluated on barium phytoextraction capacity: Brachiaria arrecta, Cyperus papyrus, Eleocharis acutangula, E. interstincta, Nephrolepsis cf. rivularis, Oryza sativa IRGA 424, O. sativa BRS Tropical, Paspalum conspersum, and Typha domingensis. Plants were grown in pots and exposed to six barium concentrations: 0, 2.5, 5.0, 10.0, 30.0, and 65.0 mg kg -1 . To simulate flooding conditions, each pot was kept with a thin water film over the soil surface (∼1.0 cm). Plants were evaluated for biomass yield and barium removal. The highest amount of barium was observed in T. domingensis biomass, followed by C. papyrus. However, the latter exported most of the barium to the aerial part of the plant, especially at higher BaCl 2 doses, while the former accumulated barium preferentially in the roots. Thus, barium removal with C. papyrus could be achieved by simply harvesting aerial biomass. The high amounts of barium in T. domingensis and C. papyrus resulted from the combination of high barium concentration in plant tissues with high biomass production. These results make T. domingensis and C. papyrus potential candidates for phytoremediation schemes to remove barium from flooded soils. Copyright © 2018 Elsevier Ltd. All rights reserved.

7 CFR 1755.506 - Aerial wire services

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 11 2011-01-01 2011-01-01 false Aerial wire services 1755.506 Section 1755.506... § 1755.506 Aerial wire services (a) Aerial services of one through six pairs shall consist of Service...), Specifications and Drawings for Service Installations at Customer Access Locations. The wire used for aerial...

Delivery and detection of dietary plant-based miRNAs in animal tissues

USDA-ARS?s Scientific Manuscript database

It has been proposed that genetic material, namely microRNAs (miRNAs), consumed in plant-based diets can affect animal gene expression. Though deep sequencing reveals the low-level presence of plant miRNAs in animal tissues, many groups have been thus far unable to replicate the finding that a rice ...

11. AERIAL VIEW LOOKING NORTH AT THE BUILDING 800 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. AERIAL VIEW LOOKING NORTH AT THE BUILDING 800 - AREA COMPLEX. ENRICHED URANIUM COMPONENTS WERE MANUFACTURED IN THIS AREA OF THE SITE. BUILDING 881, IN THE RIGHT FOREGROUND OF THE PHOTOGRAPH, WAS THE ORIGINAL PLANT B. BUILDING 883, USED FOR ROLLING AND FORMING URANIUM COMPONENTS, IS DIRECTLY TO THE NORTH OF BUILDING 881. TO THE EAST OF BUILDING 883 IS BUILDING 885, A RESEARCH AND DEVELOPMENT FACILITY FOR ALLOYS AND NON-PLUTONIUM METALS. IN THE FOREGROUND TO THE WEST OF BUILDING 881 IS AN OFFICE BUILDING, 850 (6/7/90). - Rocky Flats Plant, Bounded by Indiana Street & Routes 93, 128 & 72, Golden, Jefferson County, CO

10. AERIAL VIEW LOOKING NORTHWEST AT THE 400AREA COMPLEX. THIS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. AERIAL VIEW LOOKING NORTHWEST AT THE 400-AREA COMPLEX. THIS AREA OF THE PLANT MANUFACTURED NON-PLUTONIUM WEAPONS COMPONENTS FROM BERYLLIUM, DEPLETED URANIUM, AND STAINLESS STEEL. THE 400 - AREA ALSO INCLUDED A FACILITY FOR THE MODIFICATION OF SAFE SECURE TRANSPORT VEHICLES FOR SPECIAL NUCLEAR MATERIALS BEING SHIPPED TO AND FROM THE SITE. BUILDING 444, IN THE UPPER RIGHT EDGE OF THE PHOTOGRAPH, WAS THE ORIGINAL PLANT A. THE LARGE BUILDING IN THE TOP OF THE PHOTOGRAPH IS BUILDING 460, BUILT AS A STATE-OF-THE-ART STAINLESS STEEL MANUFACTURING FACILITY (6/27/95). - Rocky Flats Plant, Bounded by Indiana Street & Routes 93, 128 & 72, Golden, Jefferson County, CO

Demonstration of the economic feasibility of plant tissue culture for jojoba (Simmondsia chinensis) and Euphorbia spp

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sluis, C.

1980-09-01

The economic feasibility of plant tissue culture was demonstrated as applied to two plants: jojoba (Simmondsia chinensis) and Euphorbia spp. The gopher weed (Euphorbia lathyris) was selected as the species of Euphorbia to research due to the interest in this plant as a potential source of hydrocarbon-like compounds. High yield female selections of jojoba were chosen from native stands and were researched to determine the economic feasibility of mass producing these plants via a tissue culture micropropagation program. The female jojoba selection was successfully mass produced through tissue culture. Modifications in initiation techniques, as well as in multiplication media andmore » rooting parameters, were necessary to apply the tissue culture system, which had been developed for juvenile seedling tissue, to mature jojobas. Since prior attempts at transfer of tissue cultured plantlets were unsuccessful, transfer research was a major part of the project and has resulted in a system for transfer of rooted jojoba plantlets to soil. Euphorbia lathyris was successfully cultured using shoot tip cultures. Media and procedures were established for culture initiation, multiplication of shoots, callus induction and growth, and root initiation. Well-developed root systems were not attained and root initiation percentages should be increased if the system is to become commercially feasible.« less

25. Aerial photograph dated 20 June 1942, showing north end ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. Aerial photograph dated 20 June 1942, showing north end of Gould Island from the northeast (caption on photo is in error). Shop and power plant under construction at left, firing pier under construction at far right. Photo courtesy of Naval Undersea Warfare Center, Division Newport, Rhode Island. - Naval Torpedo Station, Firing Pier, North end of Gould Island in Narragansett Bay, Newport, Newport County, RI

Distribution of Potato virus Y in potato plant organs, tissues, and cells.

PubMed

Kogovšek, P; Kladnik, A; Mlakar, J; Znidarič, M Tušek; Dermastia, M; Ravnikar, M; Pompe-Novak, M

2011-11-01

The distribution of Potato virus Y (PVY) in the systemically infected potato (Solanum tuberosum) plants of the highly susceptible cultivar Igor was investigated. Virus presence and accumulation was analyzed in different plant organs and tissues using real-time polymerase chain reaction and transmission electron microscopy (TEM) negative staining methods. To get a complete insight into the location of viral RNA within the tissue, in situ hybridization was developed and optimized for the detection of PVY RNA at the cellular level. PVY was shown to accumulate in all studied leaf and stem tissues, in shoot tips, roots, and tubers; however, the level of virus accumulation was specific for each organ or tissue. The highest amounts of viral RNA and viral particles were found in symptomatic leaves and stem. By observing cell ultrastructure with TEM, viral cytoplasmic inclusion bodies were localized in close vicinity to the epidermis and in trichomes. Our results show that viral RNA, viral particles, and cytoplasmic inclusion bodies colocalize within the same type of cells or in close vicinity.

Water relations in the interaction of foliar bacterial pathogens with plants.

PubMed

Beattie, Gwyn A

2011-01-01

This review examines the many ways in which water influences the relations between foliar bacterial pathogens and plants. As a limited resource in aerial plant tissues, water is subject to manipulation by both plants and pathogens. A model is emerging that suggests that plants actively promote localized desiccation at the infection site and thus restrict pathogen growth as one component of defense. Similarly, many foliar pathogens manipulate water relations as one component of pathogenesis. Nonvascular pathogens do this using effectors and other molecules to alter hormonal responses and enhance intercellular watersoaking, whereas vascular pathogens use many mechanisms to cause wilt. Because of water limitations on phyllosphere surfaces, bacterial colonists, including pathogens, benefit from the protective effects of cellular aggregation, synthesis of hygroscopic polymers, and uptake and production of osmoprotective compounds. Moreover, these bacteria employ tactics for scavenging and distributing water to overcome water-driven barriers to nutrient acquisition, movement, and signal exchange on plant surfaces. Copyright © 2011 by Annual Reviews. All rights reserved.

Contrast-enhanced 3D micro-CT of plant tissues using different impregnation techniques.

PubMed

Wang, Zi; Verboven, Pieter; Nicolai, Bart

2017-01-01

X-ray micro-CT has increasingly been used for 3D imaging of plant structures. At the micrometer resolution however, limitations in X-ray contrast often lead to datasets with poor qualitative and quantitative measures, especially within dense cell clusters of plant tissue specimens. The current study developed protocols for delivering a cesium based contrast enhancing solution to varying plant tissue specimens for the purpose of improving 3D tissue structure characterization within plant specimens, accompanied by new image processing workflows to extract the additional data generated by the contrast enhanced scans. Following passive delivery of a 10% cesium iodide contrast solution, significant increases of 85.4 and 38.0% in analyzable cell volumes were observed in pear fruit hypanthium and tomato fruit outer mesocarp samples. A significant increase of 139.6% in the number of analyzable cells was observed in the pear fruit samples along the added ability to locate and isolate better brachysclereids and vasculature in the sample volume. Furthermore, contrast enhancement resulted in significant improvement in the definition of collenchyma and parenchyma in the petiolule of tomato leaflets, from which both qualitative and quantitative data can be extracted with respect to cell measures. However, contrast enhancement was not achieved in leaf vasculature and mesophyll tissue due to fundamental limitations. Active contrast delivery to apple fruit hypanthium samples did yield a small but insignificant increase in analyzable volume and cells, but data on vasculature can now be extracted better in correspondence to the pear hypanthium samples. Contrast delivery thus improved visualization and analysis the most in dense tissue types. The cesium based contrast enhancing protocols and workflows can be utilized to obtain detailed 3D data on the internal microstructure of plant samples, and can be adapted to additional samples of interest with minimal effort. The resulting

DRACO-STEM: An Automatic Tool to Generate High-Quality 3D Meshes of Shoot Apical Meristem Tissue at Cell Resolution

PubMed Central

Cerutti, Guillaume; Ali, Olivier; Godin, Christophe

2017-01-01

Context: The shoot apical meristem (SAM), origin of all aerial organs of the plant, is a restricted niche of stem cells whose growth is regulated by a complex network of genetic, hormonal and mechanical interactions. Studying the development of this area at cell level using 3D microscopy time-lapse imaging is a newly emerging key to understand the processes controlling plant morphogenesis. Computational models have been proposed to simulate those mechanisms, however their validation on real-life data is an essential step that requires an adequate representation of the growing tissue to be carried out. Achievements: The tool we introduce is a two-stage computational pipeline that generates a complete 3D triangular mesh of the tissue volume based on a segmented tissue image stack. DRACO (Dual Reconstruction by Adjacency Complex Optimization) is designed to retrieve the underlying 3D topological structure of the tissue and compute its dual geometry, while STEM (SAM Tissue Enhanced Mesh) returns a faithful triangular mesh optimized along several quality criteria (intrinsic quality, tissue reconstruction, visual adequacy). Quantitative evaluation tools measuring the performance of the method along those different dimensions are also provided. The resulting meshes can be used as input and validation for biomechanical simulations. Availability: DRACO-STEM is supplied as a package of the open-source multi-platform plant modeling library OpenAlea (http://openalea.github.io/) implemented in Python, and is freely distributed on GitHub (https://github.com/VirtualPlants/draco-stem) along with guidelines for installation and use. PMID:28424704

Essential oil composition of aerial parts of Micromeria persica Boiss. from Western of Shiraz, Iran.

PubMed

Jafari, Efat; Ghanbarian, Gholamabbas; Bahmanzadegan, Atefeh

2018-04-01

Micromeria persica Boiss. is medicinal and aromatic plant, belonging to the Lamiaceae family. The chemical composition of the essential oils (EOs) from aerial parts of M. persica were extracted using hydro-distillation method and analysed using GC and GC-MS. Fifty-two compounds were identified in the EOs of aerial parts of M. persica. The main chemical compositions were n-hexadecanoic acid (14.9%), thymol (9.5%), linoleic acid (8.0%), carvacrol (5.6%), (E)-nerolidol (5.5%), linolenic acid (5.5%), α-cadinol (2.7%), linalool (2.7%), borneol (2.6%), caryophyllene oxide (2.3%) and pulegone (2.0%). Presence of borneol, thymol, carvacrol and pulegone suggests the potential of this plant as a flavouring source in the food industry, being used in perfumery and cosmetics industry, vitamin E synthesis and exhibit strong fungicidal, antibacterial and antimicrobial activities.

Fate of the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) in soil and bioaccumulation in bush bean hydroponic plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harvey, S.D.; Fellows, R.J.; Cataldo, D.A.

Soils amended with [[sup 14]C]hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) were sampled over 60 d and subjected to exhaustive Soxhlet extraction followed by HPLC analysis. RDX was the only radiolabeled compound observed in soil extracts. Emission of volatile organics and [sup 14]CO[sub 2] from soil accounted for only 0.31 % of the amended radiolabel. Mass balance for RDX-amended soil was better than 84% throughout the two-month study. The analytical method developed for plants involved acid hydrolysis, solvent extraction, fractionation on Florisil adsorbent and separation by HPLC. The described methodology allowed for RDX recovery of 86 [+-] 3% from fortified bush bean leaf tissue. Furthermore » experiments were conducted with bush bean plants maintained on RDX-containing hydroponic solutions. Hydroponic plants did not emit detectable amounts of [sup 14]CO[sub 2] or radiolabeled volatile organics. Analysis of the plant tissue indicated bioaccumulation of RDX in the aerial tissues of hydroponic plants exposed for either 1 or 7 d. Metabolism of RDX to polar metabolites was observed in plants exposed for 7 d.« less

Integration of aerial imaging and variable-rate technology for site-specific aerial herbicide application

USDA-ARS?s Scientific Manuscript database

As remote sensing and variable rate technology are becoming more available for aerial applicators, practical methodologies on effective integration of these technologies are needed for site-specific aerial applications of crop production and protection materials. The objectives of this study were to...

A Protocol for Rapid, Measurable Plant Tissue Culture Using Stem Disc Meristem Micropropagation of Garlic ("Allium Sativum L.")

ERIC Educational Resources Information Center

Peat, Gerry; Jones, Meriel

2012-01-01

Plant tissue culture is becoming an important technique for the mass propagation of plants. Problems with existing techniques, such as slow growth and contamination, have restricted the practical work in plant tissue culture carried out in schools. The new protocol using garlic meristematic stem discs explained in this article addresses many of…

Chromium localization in plant tissues of Lycopersicum esculentum Mill using ICP-MS and ion microscopy (SIMS)

NASA Astrophysics Data System (ADS)

Mangabeira, Pedro Antonio; Gavrilov, Konstantin L.; Almeida, Alex-Alan Furtado de; Oliveira, Arno Heeren; Severo, Maria Isabel; Rosa, Tiago Santana; Silva, Delmira da Costa; Labejof, Lise; Escaig, Françoise; Levi-Setti, Riccardo; Mielke, Marcelo Schramm; Loustalot, Florence Grenier; Galle, Pierre

2006-03-01

High-resolution imaging secondary ion mass spectrometry (HRI-SIMS) in combination with inductively coupled plasma mass spectrometry (ICP-MS) were utilised to determine specific sites of chromium concentration in tomato plant tissues (roots, stems and leaves). The tissues were obtained from plants grown for 2 months in hydroponic conditions with Cr added in a form chromium salt (CrCl 3·6H 2O) to concentrations of 25 and 50 mg/L. The chemical fixation procedure used permit to localize only insoluble or strongly bound Cr components in tomato plant tissue. In this work no quantitative SIMS analysis was made. HRI-SIMS analysis revealed that the transport of chromium is restricted to the vascular system of roots, stems and leaves. No Cr was detected in epidermis, palisade parenchyma and spongy parenchyma cells of the leaves. The SIMS-300 spectra obtained from the tissues confirm the HRI-SIMS observations. The roots, and especially walls of xylem vessels, were determined as the principal site of chromium accumulation in tomato plants.

Mechanisms and prevention of plant tissue collapse during dehydration: a critical review.

PubMed

Prothon, Frédéric; Ahrné, Lilia; Sjöholm, Ingegerd

2003-01-01

The appearance and functional properties are primordial in the quality assessment of semifinished fruit and vegetable products. These properties are often associated with shrunken, shriveled, darkened materials of poor rehydration ability after been subjected to air-drying--the most used drying method in the food industry. Fruits and vegetables are cellular tissues containing gas-filled pores that tend to collapse when subjected to dehydration. Collapse is an overall term that has different meanings and scale-settings in the literature depending on whether the author is a plant physiologist, a food technologist, a chemical engineer, or a material scientist. Some clarifications are given in this particular but wide field. The purpose of this work was to make a state-of-the-art contribution to the structural and textural effects of different types of dehydration on edible plant products and give a basis for preventing this phenomenon. The plant tissue is described, and the primordial role of the cell wall in keeping the structural integrity is emphasized. Water and its functionality at macro and micro levels of the cellular tissue are reviewed as well as its transport during dehydration. The effects of both dehydration and rehydration are described in detail, and the term "textural collapse" is proposed as an alternative to structural collapse.

Tissue age and plant genotype affect the microbiota of apple and pear bark.

PubMed

Arrigoni, Elena; Antonielli, Livio; Pindo, Massimo; Pertot, Ilaria; Perazzolli, Michele

2018-06-01

Plant tissues host complex fungal and bacterial communities, and their composition is determined by host traits such as tissue age, plant genotype and environmental conditions. Despite the importance of bark as a possible reservoir of plant pathogenic microorganisms, little is known about the associated microbial communities. In this work, we evaluated the composition of fungal and bacterial communities in the pear (Abate and Williams cultivars) and apple (Golden Delicious and Gala cultivars) bark of three/four-year-old shoots (old bark) or one-year-old shoots (young bark), using a meta-barcoding approach. The results showed that both fungal and bacterial communities are dominated by genera with ubiquitous attitudes, such as Aureobasidium, Cryptococcus, Deinococcus and Hymenobacter, indicating intense microbial migration to surrounding environments. The shoot age, plant species and plant cultivar influenced the composition of bark fungal and bacterial communities. In particular, bark communities included potential biocontrol agents that could maintain an equilibrium with potential plant pathogens. The abundance of fungal (e.g. Alternaria, Penicillium, Rosellinia, Stemphylium and Taphrina) and bacterial (e.g. Curtobacterium and Pseudomonas) plant pathogens was affected by bark age and host genotype, as well as those of fungal genera (e.g. Arthrinium, Aureobasidium, Rhodotorula, Sporobolomyces) and bacterial genera (e.g. Bacillus, Brevibacillus, Methylobacterium, Sphingomonas and Stenotrophomonas) with possible biocontrol and plant growth promotion properties. Copyright © 2018 Elsevier GmbH. All rights reserved.

The Art of Aerial Warfare

DTIC Science & Technology

2005-03-01

14 3 THE POLITICAL DIMENSIONS OF AERIAL WARFARE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 How Political Effects in...Aerial Warfare . . . . . . Outweigh Military Effects . . . . . . . . . . . . . . . 19 Political Targets Versus Military Targets . . . . . 22...34 4 MILITARY AND POLITICAL EFFECTS OF STRATEGIC ATTACK . . . . . . . . . . . . . . . . . . 35 The Premise of

Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants.

PubMed

Sarmast, Mostafa Khoshhal; Salehi, Hassan; Khosh-Khui, Morteza

2014-12-01

Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these plantlets is the most important step in tissue culture techniques. An experiment was conducted under in vitro conditions to study the effects of shaking duration (twice daily at 6:00 a.m. and 9:00 p.m. for 2, 4, 8, and 16 min at 250 rpm for 14 days) on Sansevieria trifasciata L. as a model plant. Results showed that shaking improved handling, total plant height, and leaf characteristics of the model plant. Forty-eight hours after 14 days of shaking treatments with increasing shaking time, leaf length decreased but proline content of leaf increased. However, 6 months after starting the experiment different results were observed. In explants that received 16 min of shaking treatment, leaf length and area and photosynthesis rate were increased compared with control plantlets. Six months after starting the experiment, control plantlets had 12.5 % mortality; however, no mortality was observed in other treated explants. The results demonstrated that shaking improved the explants' root length and number and as a simple, cost-effective, and non-chemical novel approach may be substituted for other prevalent acclimatization techniques used for tissue culture regenerated plantlets. Further studies with sensitive plants are needed to establish this hypothesis.

47 CFR 32.6431 - Aerial wire expense.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false Aerial wire expense. 32.6431 Section 32.6431... FOR TELECOMMUNICATIONS COMPANIES Instructions for Expense Accounts § 32.6431 Aerial wire expense. This account shall include expenses associated with aerial wire. ...

47 CFR 32.6431 - Aerial wire expense.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 2 2011-10-01 2011-10-01 false Aerial wire expense. 32.6431 Section 32.6431... FOR TELECOMMUNICATIONS COMPANIES Instructions for Expense Accounts § 32.6431 Aerial wire expense. This account shall include expenses associated with aerial wire. ...

Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7

PubMed Central

Prieto, Pilar; Navarro‐Raya, Carmen; Valverde‐Corredor, Antonio; Amyotte, Stefan G.; Dobinson, Katherine F.; Mercado‐Blanco, Jesús

2009-01-01

Summary The colonization process of Olea europaea by the defoliating pathotype of Verticillium dahliae, and the in planta interaction with the endophytic, biocontrol strain Pseudomonas fluorescens PICF7 were determined. Differential fluorescent protein tagging was used for the simultaneous visualization of P. fluorescens PICF7 and V. dahliae in olive tissues. Olive plants were bacterized with PICF7 and then transferred to V. dahliae‐infested soil. Monitoring olive colonization events by V. dahliae and its interaction with PICF7 was conducted using a non‐gnotobiotic system, confocal laser scanner microscopy and tissue vibratoming sections. A yellow fluorescently tagged V. dahliae derivative (VDAT‐36I) was obtained by Agrobacterium tumefaciens‐mediated transformation. Isolate VDAT‐36I quickly colonized olive root surface, successfully invaded root cortex and vascular tissues via macro‐ and micro‐breakages, and progressed to the aerial parts of the plant through xylem vessel cells. Strain PICF7 used root hairs as preferred penetration site, and once established on/in root tissues, hindered pathogen colonization. For the first time using this approach, the entire colonization process of a woody plant by V. dahliae is reported. Early and localized root surface and root endophytic colonization by P. fluorescens PICF7 is needed to impair full progress of verticillium wilt epidemics in olive. PMID:21255281

Aerial thermography for energy conservation

NASA Technical Reports Server (NTRS)

Jack, J. R.

1978-01-01

Thermal infrared scanning from an aircraft is a convenient and commercially available means for determining relative rates of energy loss from building roofs. The need to conserve energy as fuel costs makes the mass survey capability of aerial thermography an attractive adjunct to community energy awareness programs. Background information on principles of aerial thermography is presented. Thermal infrared scanning systems, flight and environmental requirements for data acquisition, preparation of thermographs for display, major users and suppliers of thermography, and suggested specifications for obtaining aerial scanning services were reviewed.

Phytochemical screening and chemical variability in volatile oils of aerial parts of Morinda morindoides.

PubMed

Kiazolu, J Boima; Intisar, Azeem; Zhang, Lingyi; Wang, Yun; Zhang, Runsheng; Wu, Zhongping; Zhang, Weibing

2016-10-01

Morinda morindoides is an important Liberian traditional medicine for the treatment of malaria, fever, worms etc. The plant was subjected to integrated approaches including phytochemical screening and gas chromatography mass spectrometry (GC-MS) analyses. Phytochemical investigation of the powdered plant revealed the presence of phenolics, tannins, flavonoids, saponins, terpenes, steroidal compounds and volatile oil. Steam distillation followed by GC-MS resulted in the identification of 47 volatiles in its aerial parts: 28 were in common including various bioactive volatiles. Major constituents of leaves were phytol (43.63%), palmitic acid (8.55%) and geranyl linalool (6.95%) and stem were palmitic acid (14.95%), eicosane (9.67%) and phytol (9.31%), and hence, a significant difference in the percentage composition of aerial parts was observed. To study seasonal changes, similarity analysis was carried out by calculating correlation coefficient (r) and vector angle cosine (z) that were more than 0.91 for stem-to-stem and leaf-to-leaf batches indicating considerable consistency.

Exploring plant tissue culture in Withania somnifera (L.) Dunal: in vitro propagation and secondary metabolite production.

PubMed

Shasmita; Rai, Manoj K; Naik, Soumendra K

2017-12-26

Withania somnifera (L.) Dunal (family: Solanaceae), commonly known as "Indian Ginseng", is a medicinally and industrially important plant of the Indian subcontinent and other warmer parts of the world. The plant has multi-use medicinal potential and has been listed among 36 important cultivated medicinal plants of India that are in high demand for trade due to its pharmaceutical uses. The medicinal importance of this plant is mainly due to the presence of different types of steroidal lactones- withanolides in the roots and leaves. Owing to low seed viability and poor germination, the conventional propagation of W. somnifera falls short to cater its commercial demands particularly for secondary metabolite production. Therefore, there is a great need to develop different biotechnological approaches through tissue and organ culture for seasonal independent production of plants in large scale which will provide sufficient raw materials of uniform quality for pharmaceutical purposes. During past years, a number of in vitro plant regeneration protocols via organogenesis and somatic embryogenesis and in vitro conservation through synthetic seed based encapsulation technology have been developed for W. somnifera. Several attempts have also been made to standardize the protocol of secondary metabolite production via tissue/organ cultures, cell suspension cultures, and Agrobacterium rhizogenes-mediated transformed hairy root cultures. Employment of plant tissue culture based techniques would provide means for rapid propagation and conservation of this plant species and also provide scope for enhanced production of different bioactive secondary metabolites. The present review provides a comprehensive report on research activities conducted in the area of tissue culture and secondary metabolite production in W. somnifera during the past years. It also discusses the unexplored areas which might be taken into consideration for future research so that the medicinal properties and

29. Aerial photograph (1973) looking south across Gould Island. Firing ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. Aerial photograph (1973) looking south across Gould Island. Firing pier (still possessing third and fourth levels) in foreground. Pitched roof extending from south end of firing pier marks location of frame approach between pier and shop building (center rear) and power plant (to right of shop). Photo courtesy of Naval Undersea Warfare Center, Division Newport, Rhode Island. - Naval Torpedo Station, Firing Pier, North end of Gould Island in Narragansett Bay, Newport, Newport County, RI

Electrophoretic Detection and Confocal Microscopic Imaging of Tyrosine Nitrated Proteins in Plant Tissue.

PubMed

Arora, Dhara; Singh, Neha; Bhatla, Satish C

2018-01-01

Tyrosine nitrated proteins can be detected in plant cells electrophoretically and their distribution can be monitored by confocal laser scanning microscopy (CLSM) imaging. One-dimensional polyacrylamide gel electrophoresis (1D PAGE) followed by Western blotting using polyclonal antibody against 3-nitrotyrosine residues enables detection of tyrosine nitrated proteins in plant cells. Here we describe detection of tyrosine nitrated proteins in the homogenates derived from sunflower (Helianthus annuus L.) seedling cotyledons. Total soluble proteins obtained from tissue homogenates are resolved using vertical gel electrophoresis followed by their electrophoretic transfer on to a microporous membrane support for immunodetection. Spatial distribution of tyrosine nitrated proteins can be visualized using an antibody against 3-nitrotyrosine residues. Immunofluorescent localization is performed by cutting 7 μm thick wax sections of tissue followed by incubation in primary anti-nitrotyrosine antibody (dilution 1:200) and secondary Cy-3 labeled anti-rabbit IgG antibody (dilution 1:1500). Confocal laser scanning microscopy analysis is undertaken using argon lasers (ex: 530-550 nm and em: 570 nm) at pinhole 1. Modulation in the abundance and spatial localization of tyrosine nitrated proteins in plant tissues can be monitored using these techniques.

ASSESSMENT OF MOLECULAR GENETIC STABILITY BETWEEN LONG-TERM CRYOPRESERVED AND TISSUE CULTURED WASABI (Wasabia japonica) PLANTS.

PubMed

Maki, S; Hirai, Y; Niino, T; Matsumoto, T

2015-01-01

Maintaining the genetic integrity in long-term tissue cultured and cryopreserved plants is important for the conservation of plant genetic resources. In this study, the genetic stability of cryopreserved wasabi shoot tips stored for 10 years at -150 degree C was visualized using Amplified Fragment Length Polymorphism (AFLP) and Methylation Sensitive Amplified Polymorphism (MSAP). The study included plants derived from cryopreserved shoot tips after 10.5 years storage at -150 degree C (LN10yr), after 2 h storage at -196 degree C (LN2hr), cryopreservation controls (No LN cooling (TC)) and non-treated controls without LN cooling (LC). The donor plants for LN2hr, TC and LC were also maintained in vitro at 20 degree C for the same period. Neither technique detected genetic variations in either control or cryopreserved plants. Some mutations were noted in plants maintained in tissue culture for 10 years. Comparison of genome stability for TC and LN2hr plants showed only a minor change in DNA. However, when comparing the LC and Ln10yr, many differences were found. We conclude that cryopreservation is a superior conservation method compared to tissue culture in maintaining genetic stability for a long-term storage of wasabi germplasm.

A workflow to preserve genome-quality tissue samples from plants in botanical gardens and arboreta.

PubMed

Gostel, Morgan R; Kelloff, Carol; Wallick, Kyle; Funk, Vicki A

2016-09-01

Internationally, gardens hold diverse living collections that can be preserved for genomic research. Workflows have been developed for genomic tissue sampling in other taxa (e.g., vertebrates), but are inadequate for plants. We outline a workflow for tissue sampling intended for two audiences: botanists interested in genomics research and garden staff who plan to voucher living collections. Standard herbarium methods are used to collect vouchers, label information and images are entered into a publicly accessible database, and leaf tissue is preserved in silica and liquid nitrogen. A five-step approach for genomic tissue sampling is presented for sampling from living collections according to current best practices. Collecting genome-quality samples from gardens is an economical and rapid way to make available for scientific research tissue from the diversity of plants on Earth. The Global Genome Initiative will facilitate and lead this endeavor through international partnerships.

Isolation of viable multicellular glands from tissue of the carnivorous plant, Nepenthes.

PubMed

Rottloff, Sandy; Mithöfer, Axel; Müller, Ute; Kilper, Roland

2013-12-22

Many plants possess specialized structures that are involved in the production and secretion of specific low molecular weight compounds and proteins. These structures are almost always localized on plant surfaces. Among them are nectaries or glandular trichomes. The secreted compounds are often employed in interactions with the biotic environment, for example as attractants for pollinators or deterrents against herbivores. Glands that are unique in several aspects can be found in carnivorous plants. In so-called pitcher plants of the genus Nepenthes, bifunctional glands inside the pitfall-trap on the one hand secrete the digestive fluid, including all enzymes necessary for prey digestion, and on the other hand take-up the released nutrients. Thus, these glands represent an ideal, specialized tissue predestinated to study the underlying molecular, biochemical, and physiological mechanisms of protein secretion and nutrient uptake in plants. Moreover, generally the biosynthesis of secondary compounds produced by many plants equipped with glandular structures could be investigated directly in glands. In order to work on such specialized structures, they need to be isolated efficiently, fast, metabolically active, and without contamination with other tissues. Therefore, a mechanical micropreparation technique was developed and applied for studies on Nepenthes digestion fluid. Here, a protocol is presented that was used to successfully prepare single bifunctional glands from Nepenthes traps, based on a mechanized microsampling platform. The glands could be isolated and directly used further for gene expression analysis by PCR techniques after preparation of RNA.

The use of color infrared aerial photography in determining salt marsh vegetation and delimiting man-made structures of Lynnhaven Bay, Virginia. M.S. Thesis

NASA Technical Reports Server (NTRS)

Holman, R. E., III

1974-01-01

Color infrared aerial photography was found to be superior to color aerial photography in an ecological study of Lynnhaven Bay, Virginia. The research was divided into three phases: (1) Determination of the feasibility of correlating color infrared aerial photography with saline wetland species composition and zonation patterns, (2) determination of the accuracy of the aerial interpretation and problems related to the aerial method used; and (3) comparison of developed with undeveloped areas along Lynnhaven Bay's shoreline. Wetland species composition and plant community zonation bands were compared with aerial infrared photography and resulted in a high degree of correlation. Problems existed with changing physical conditions; time of day, aircraft angle and sun angle, making it necessary to use several different characteristics in wetland species identification. The main characteristics used were known zonation patterns, textural signatures and color tones. Lynnhaven Bay's shoreline was 61.5 percent developed.

CFD Simulation of Aerial Crop Spraying

NASA Astrophysics Data System (ADS)

Omar, Zamri; Qiang, Kua Yong; Mohd, Sofian; Rosly, Nurhayati

2016-11-01

Aerial crop spraying, also known as crop dusting, is made for aerial application of pesticides or fertilizer. An agricultural aircraft which is converted from an aircraft has been built to combine with the aerial crop spraying for the purpose. In recent years, many studies on the aerial crop spraying were conducted because aerial application is the most economical, large and rapid treatment for the crops. The main objective of this research is to study the airflow of aerial crop spraying system using Computational Fluid Dynamics. This paper is focus on the effect of aircraft speed and nozzle orientation on the distribution of spray droplet at a certain height. Successful and accurate of CFD simulation will improve the quality of spray during the real situation and reduce the spray drift. The spray characteristics and efficiency are determined from the calculated results of CFD. Turbulence Model (k-ɛ Model) is used for the airflow in the fluid domain to achieve a more accurate simulation. Furthermore, spray simulation is done by setting the Flat-fan Atomizer Model of Discrete Phase Model (DPM) at the nozzle exit. The interaction of spray from each flat-fan atomizer can also be observed from the simulation. The evaluation of this study is validation and grid dependency study using field data from industry.

A novel resource-service mutualism between bats and pitcher plants.

PubMed

Grafe, T Ulmar; Schöner, Caroline R; Kerth, Gerald; Junaidi, Anissa; Schöner, Michael G

2011-06-23

Mutualistic relationships between vertebrates and plants apart from the pollen and seed-dispersal syndromes are rare. At first view, carnivorous pitcher plants of the genus Nepenthes seem to be highly unlikely candidates for mutualistic interactions with animals, as they form dimorphic terrestrial and aerial pitchers that trap arthropods and small vertebrates. Surprisingly, however, the aerial pitchers of Nepenthes rafflesiana variety elongata are poor insect traps, with low amounts of insect-attractive volatile compounds and low amounts of digestive fluid. Here, we show that N. rafflesiana elongata gains an estimated 33.8 per cent of the total foliar nitrogen from the faeces of Hardwicke's woolly bats (Kerivoula hardwickii hardwickii) that exclusively roost in its aerial pitchers. This is the first case in which the faeces-trapping syndrome has been documented in a pitcher plant that attracts bats and only the second case of a mutualistic association between a carnivorous plant and a mammal to date.

Comparative Analysis of the Tour Jete and Aerial with Detailed Analysis of Aerial Takeoff Mechanics

NASA Astrophysics Data System (ADS)

Pierson, Mimi; Coplin, Kim

2006-10-01

Whether internally as muscle tension or from external sources, forces are necessary for all motion. This research focused on athletic rotations where conditions of flight are established during takeoff. By studying reaction forces that produce torques, moments of inertia, and linear and angular differences between distinct rotations around different principle axes of the body (tour jete in ballet - longitudinal axis; aerial in gymnastics - anteroposterior axis), and by looking at the values of angular momentum in the specific mechanics of aerial takeoff, we can gain insight into possible causes of injury, flaws in technique and limitations of athletes. Results showed significant differences in the horizontal and vertical components of takeoff between the tour jete and the aerial, and a realization that torque was produced in different biomechanical planes. Both rotations showed braking forces before takeoff to counteract forward momentum and increase vertical lift, but the angle of applied force varied, and the horizontal components of velocity and force and vertical velocity as well as moment of inertia throughout flight were consistently greater for the aerial. Breakdown of aerial takeoff highlighted the relative importance of the takeoff phases, showing that completion depends fundamentally upon the rotation of the rear foot and torso twisting during takeoff rather than the last foot in contact with the ground.

Aerial 3D display by use of a 3D-shaped screen with aerial imaging by retro-reflection (AIRR)

NASA Astrophysics Data System (ADS)

Kurokawa, Nao; Ito, Shusei; Yamamoto, Hirotsugu

2017-06-01

The purpose of this paper is to realize an aerial 3D display. We design optical system that employs a projector below a retro-reflector and a 3D-shaped screen. A floating 3D image is formed with aerial imaging by retro-reflection (AIRR). Our proposed system is composed of a 3D-shaped screen, a projector, a quarter-wave retarder, a retro-reflector, and a reflective polarizer. Because AIRR forms aerial images that are plane-symmetric of the light sources regarding the reflective polarizer, the shape of the 3D screen is inverted from a desired aerial 3D image. In order to expand viewing angle, the 3D-shaped screen is surrounded by a retro-reflector. In order to separate the aerial image from reflected lights on the retro- reflector surface, the retro-reflector is tilted by 30 degrees. A projector is located below the retro-reflector at the same height of the 3D-shaped screen. The optical axis of the projector is orthogonal to the 3D-shaped screen. Scattered light on the 3D-shaped screen forms the aerial 3D image. In order to demonstrate the proposed optical design, a corner-cube-shaped screen is used for the 3D-shaped screen. Thus, the aerial 3D image is a cube that is floating above the reflective polarizer. For example, an aerial green cube is formed by projecting a calculated image on the 3D-shaped screen. The green cube image is digitally inverted in depth by our developed software. Thus, we have succeeded in forming aerial 3D image with our designed optical system.

Pathogen and biological contamination management in plant tissue culture: phytopathogens, vitro pathogens, and vitro pests.

PubMed

Cassells, Alan C

2012-01-01

The ability to establish and grow plant cell, organ, and tissue cultures has been widely exploited for basic and applied research, and for the commercial production of plants (micro-propagation). Regardless of whether the application is for research or commerce, it is essential that the cultures be established in vitro free of biological contamination and be maintained as aseptic cultures during manipulation, growth, and storage. The risks from microbial contamination are spurious experimental results due to the effects of latent contaminants or losses of valuable experimental or commercial cultures. Much of the emphasis in culture contamination management historically focussed on the elimination of phytopathogens and the maintenance of cultures free from laboratory contamination by environmental bacteria, fungi (collectively referred to as "vitro pathogens", i.e. pathogens or environmental micro-organisms which cause culture losses), and micro-arthropods ("vitro pests"). Microbial contamination of plant tissue cultures is due to the high nutrient availability in the almost universally used Murashige and Skoog (Physiol Plant 15:473-497, 1962) basal medium or variants of it. In recent years, it has been shown that many plants, especially perennials, are at least locally endophytically colonized intercellularly by bacteria. The latter, and intracellular pathogenic bacteria and viruses/viroids, may pass latently into culture and be spread horizontally and vertically in cultures. Growth of some potentially cultivable endophytes may be suppressed by the high salt and sugar content of the Murashige and Skoog basal medium and suboptimal temperatures for their growth in plant tissue growth rooms. The management of contamination in tissue culture involves three stages: disease screening (syn. disease indexing) of the stock plants with disease and endophyte elimination where detected; establishment and pathogen and contaminant screening of established initial cultures

28. AERIAL VIEW LOOKING EAST AT THE WEST GATE IN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

28. AERIAL VIEW LOOKING EAST AT THE WEST GATE IN 1978. SHOWN IS BUILDING 100, THE MAIN ENTRANCE POINT TO THE SITE FROM 1969 UNTIL 1985. DURING THIS TIME EACH AUTOMOBILE THAT ENTERED THE SITE WAS SEARCHED. IN 1985, BUILDING 120 WAS BUILT AT THE OUTERMOST WEST EDGE OF THE SITE. THERE WERE 29 FACILITIES AROUND THE SITE DEDICATED TO SECURITY (5/4/78). - Rocky Flats Plant, Bounded by Indiana Street & Routes 93, 128 & 72, Golden, Jefferson County, CO

Evaluation of medicinal plants from Central Kalimantan for antimelanogenesis.

PubMed

Arung, Enos Tangke; Kusuma, Irawan Wijaya; Christy, Eva Oktoberiani; Shimizu, Kuniyoshi; Kondo, Ryuichiro

2009-10-01

In the course of searching for new materials to use as whitening agents, we screened 19 methanol extracts prepared from 14 medicinal plants from Central Kalimantan province, Indonesia. The screening methods used were the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, a tyrosinase inhibition assay, and a melanin formation inhibition assay using B16 melanoma cells. The extracts of Willughbeia coriacea (bark part of aerial root), Phyllanthus urinaria (root), Eleutherine palmifolia (bulb), Eusideroxylon zwageri (seed), Dendrophthoe petandra (aerial root), Passiflora foetida (stem), and Vitex pinnata (root) showed DPPH radical-scavenging activity of more than 70% at 100 microg/ml. The extracts of W. coriacea (bark part of aerial root), P. urinaria (root), and D. petandra (aerial root) showed tyrosinase inhibitory activity of more than 40% using L-tyrosine as a substrate at 500 microg/ml. The extracts of W. coriacea (bark part of aerial root) and D. petandra (aerial root) showed tyrosinase inhibitory activity of more than 40% using L-DOPA as a substrate at 500 microg/ml. The extracts of W. coriacea (bark part of aerial root, 200 microg/ml), Glochidion philippcum (aerial root, 200 and 300 microg/ml), E. palmifolia (bulb, 50 microg/ml), E. zwageri (seed, 100 microg/ml), D. petandra (aerial root, 200 microg/ml), Lansium domesticum (bark, 25 microg/ml), P. foetida (stem, fruit, 300 microg/ml), and Solanum torvum (root, 300 microg/ml) strongly inhibited the melanin production of B16 melanoma cells without significant cytotoxicity. These findings indicate that some medicinal plants from Central Kalimantan are potential ingredients for skin-whitening cosmetics if their safety can be confirmed.

Plant Growth Promoting and Biocontrol Activity of Streptomyces spp. as Endophytes.

PubMed

Vurukonda, Sai Shiva Krishna Prasad; Giovanardi, Davide; Stefani, Emilio

2018-03-22

There has been many recent studies on the use of microbial antagonists to control diseases incited by soilborne and airborne plant pathogenic bacteria and fungi, in an attempt to replace existing methods of chemical control and avoid extensive use of fungicides, which often lead to resistance in plant pathogens. In agriculture, plant growth-promoting and biocontrol microorganisms have emerged as safe alternatives to chemical pesticides. Streptomyces spp. and their metabolites may have great potential as excellent agents for controlling various fungal and bacterial phytopathogens. Streptomycetes belong to the rhizosoil microbial communities and are efficient colonizers of plant tissues, from roots to the aerial parts. They are active producers of antibiotics and volatile organic compounds, both in soil and in planta , and this feature is helpful for identifying active antagonists of plant pathogens and can be used in several cropping systems as biocontrol agents. Additionally, their ability to promote plant growth has been demonstrated in a number of crops, thus inspiring the wide application of streptomycetes as biofertilizers to increase plant productivity. The present review highlights Streptomyces spp.-mediated functional traits, such as enhancement of plant growth and biocontrol of phytopathogens.

Aerial detection surveys in the United States

Treesearch

E. W. Johnson; D. Wittwer

2006-01-01

Aerial detection surveys, also known as aerial sketchmapping, is a remote sensing technique of observing forest change events from an aircraft and documenting them manually onto a map. Data from aerial surveys have become an important component of the Forest Health Monitoring, a national program designed to determine the status, changes, and trends in indicators of...

Multitemporal field-based plant height estimation using 3D point clouds generated from small unmanned aerial systems high-resolution imagery

NASA Astrophysics Data System (ADS)

Malambo, L.; Popescu, S. C.; Murray, S. C.; Putman, E.; Pugh, N. A.; Horne, D. W.; Richardson, G.; Sheridan, R.; Rooney, W. L.; Avant, R.; Vidrine, M.; McCutchen, B.; Baltensperger, D.; Bishop, M.

2018-02-01

Plant breeders and agronomists are increasingly interested in repeated plant height measurements over large experimental fields to study critical aspects of plant physiology, genetics and environmental conditions during plant growth. However, collecting such measurements using commonly used manual field measurements is inefficient. 3D point clouds generated from unmanned aerial systems (UAS) images using Structure from Motion (SfM) techniques offer a new option for efficiently deriving in-field crop height data. This study evaluated UAS/SfM for multitemporal 3D crop modelling and developed and assessed a methodology for estimating plant height data from point clouds generated using SfM. High-resolution images in visible spectrum were collected weekly across 12 dates from April (planting) to July (harvest) 2016 over 288 maize (Zea mays L.) and 460 sorghum (Sorghum bicolor L.) plots using a DJI Phantom 3 Professional UAS. The study compared SfM point clouds with terrestrial lidar (TLS) at two dates to evaluate the ability of SfM point clouds to accurately capture ground surfaces and crop canopies, both of which are critical for plant height estimation. Extended plant height comparisons were carried out between SfM plant height (the 90th, 95th, 99th percentiles and maximum height) per plot and field plant height measurements at six dates throughout the growing season to test the repeatability and consistency of SfM estimates. High correlations were observed between SfM and TLS data (R2 = 0.88-0.97, RMSE = 0.01-0.02 m and R2 = 0.60-0.77 RMSE = 0.12-0.16 m for the ground surface and canopy comparison, respectively). Extended height comparisons also showed strong correlations (R2 = 0.42-0.91, RMSE = 0.11-0.19 m for maize and R2 = 0.61-0.85, RMSE = 0.12-0.24 m for sorghum). In general, the 90th, 95th and 99th percentile height metrics had higher correlations to field measurements than the maximum metric though differences among them were not statistically significant. The

Fungal endophytes in aboveground tissues of desert plants: infrequent in culture, but highly diverse and distinctive symbionts.

PubMed

Massimo, Nicholas C; Nandi Devan, M M; Arendt, Kayla R; Wilch, Margaret H; Riddle, Jakob M; Furr, Susan H; Steen, Cole; U'Ren, Jana M; Sandberg, Dustin C; Arnold, A Elizabeth

2015-07-01

In hot deserts, plants cope with aridity, high temperatures, and nutrient-poor soils with morphological and biochemical adaptations that encompass intimate microbial symbioses. Whereas the root microbiomes of arid-land plants have received increasing attention, factors influencing assemblages of symbionts in aboveground tissues have not been evaluated for many woody plants that flourish in desert environments. We evaluated the diversity, host affiliations, and distributions of endophytic fungi associated with photosynthetic tissues of desert trees and shrubs, focusing on nonsucculent woody plants in the species-rich Sonoran Desert. To inform our strength of inference, we evaluated the effects of two different nutrient media, incubation temperatures, and collection seasons on the apparent structure of endophyte assemblages. Analysis of >22,000 tissue segments revealed that endophytes were isolated four times more frequently from photosynthetic stems than leaves. Isolation frequency was lower than expected given the latitude of the study region and varied among species a function of sampling site and abiotic factors. However, endophytes were very species-rich and phylogenetically diverse, consistent with less arid sites of a similar latitudinal position. Community composition differed among host species, but not as a function of tissue type, sampling site, sampling month, or exposure. Estimates of abundance, diversity, and composition were not influenced by isolation medium or incubation temperature. Phylogenetic analyses of the most commonly isolated genus (Preussia) revealed multiple evolutionary origins of desert-plant endophytism and little phylogenetic structure with regard to seasonality, tissue preference, or optimal temperatures and nutrients for growth in vitro. Together, these results provide insight into endophytic symbioses in desert-plant communities and can be used to optimize strategies for capturing endophyte biodiversity at regional scales.

Plant tissue analysis for explosive compounds in phytoremediation and phytoforensics.

PubMed

Karnjanapiboonwong, Adcharee; Mu, Ruipu; Yuan, Yuan; Shi, Honglan; Ma, Yinfa; Burken, Joel G

2012-01-01

Plant tissue analysis methods were evaluated for six explosive compounds to assess uptake and phytoforensic methods development to quantify explosives in plant to obtain the plant data for the evaluation of explosive contamination in soil and groundwater. Four different solvent mixtures containing acetonitrile or methanol were tested at variable extraction ratios to compare the extraction efficiency for six explosive compounds: 2,4,6-trinitrotoluene (TNT), pentaerythritoltetranitrate (PETN), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), 2-amino-4,6-dinitrotoluene (2ADNT), and 2,4-Dinitroanisole (DNAN), in Laurel Willow (Salix pentandra) stem and range grass Big Bluestem (Andropogon gerardii) using LC-MS/MS. Plant tissues were spiked with 500 ng/g of explosives and extracted using ultrasonically-assisted solvent extraction. With the ratio of fresh plant mass to solvent volume of 1:20 for willow and 1:40 for big bluestem grass, results indicated that all explosives in willow except HMX were extracted at higher than 73.3% by using 20 mL of methanol, 50:50 (v/v) methanol:water, or acetonitrile, whereas HMX was extracted with the highest recovery of 61.3% by 20 mL of acetonitrile. In big bluestem grass, the most effective solvents were 20 mL of either methanol or 50:50 (v/v) methanol:water for PETN extraction with a recovery of higher than 101.2% and 20 mL of 50:50 (v/v) methanol:water for HMX, RDX, TNT, 2ADNT, and DNAN extraction with a recovery of 83.8%, 104.4%, 97.5%, 80.7%, and 108.2%, respectively. However, unlike methanol and acetonitrile, 50:50 (v/v) methanol:water provided no problem of leading or split peak in chromatogram; therefore, it was preferred in the test and performed a method validation. Results indicated that 50:50 (v/v) methanol:water provided good repeatability and recovery and method detection limits at 0.5-20 ng/g fresh weight or 8.8-61.3 ng/g dry weight. Overall, results suggested that

Keeping the rhythm: light/dark cycles during postharvest storage preserve the tissue integrity and nutritional content of leafy plants.

PubMed

Liu, John D; Goodspeed, Danielle; Sheng, Zhengji; Li, Baohua; Yang, Yiran; Kliebenstein, Daniel J; Braam, Janet

2015-03-27

The modular body structure of plants enables detached plant organs, such as postharvest fruits and vegetables, to maintain active responsiveness to environmental stimuli, including daily cycles of light and darkness. Twenty-four hour light/darkness cycles entrain plant circadian clock rhythms, which provide advantage to plants. Here, we tested whether green leafy vegetables gain longevity advantage by being stored under light/dark cycles designed to maintain biological rhythms. Light/dark cycles during postharvest storage improved several aspects of plant tissue performance comparable to that provided by refrigeration. Tissue integrity, green coloration, and chlorophyll content were generally enhanced by cycling of light and darkness compared to constant light or darkness during storage. In addition, the levels of the phytonutrient glucosinolates in kale and cabbage remained at higher levels over time when the leaf tissue was stored under light/dark cycles. Maintenance of the daily cycling of light and dark periods during postharvest storage may slow the decline of plant tissues, such as green leafy vegetables, improving not only appearance but also the health value of the crops through the maintenance of chlorophyll and phytochemical content after harvest.

Hydraulics of Asteroxylon mackei, an early Devonian vascular plant, and the early evolution of water transport tissue in terrestrial plants.

PubMed

Wilson, J P; Fischer, W W

2011-03-01

The core of plant physiology is a set of functional solutions to a tradeoff between CO(2) acquisition and water loss. To provide an important evolutionary perspective on how the earliest land plants met this tradeoff, we constructed a mathematical model (constrained geometrically with measurements of fossils) of the hydraulic resistance of Asteroxylon, an Early Devonian plant. The model results illuminate the water transport physiology of one of the earliest vascular plants. Results show that Asteroxylon's vascular system contains cells with low hydraulic resistances; these resistances are low because cells were covered by scalariform pits, elliptical structures that permit individual cells to have large areas for water to pass from one cell to another. Asteroxylon could move a large amount of water quickly given its large pit areas; however, this would have left these plants particularly vulnerable to damage from excessive evapotranspiration. These results highlight a repeated pattern in plant evolution, wherein the evolution of highly conductive vascular tissue precedes the appearance of adaptations to increase water transport safety. Quantitative insight into the vascular transport of Asteroxylon also allows us to reflect on the quality of CO(2) proxy estimates based on early land plant fossils. Because Asteroxylon's vascular tissue lacked any safety features to prevent permanent damage, it probably used stomatal abundance and behavior to prevent desiccation. If correct, low stomatal frequencies in Asteroxylon reflect the need to limit evapotranspiration, rather than adaptation to high CO(2) concentrations in the atmosphere. More broadly, methods to reveal and understand water transport in extinct plants have a clear use in testing and bolstering fossil plant-based paleoclimate proxies. © 2011 Blackwell Publishing Ltd.

Biological screening of some Turkish medicinal plant extracts for antimicrobial and toxicity activities.

PubMed

Turker, A U; Usta, C

2008-01-20

Screening of antibacterial activity and toxicity of 22 aqueous plant extracts from 17 Turkish plants was conducted. Antibacterial activity was performed with six bacteria including Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus and Staphylococcus epidermidis. Extracts of Tussilago farfara leaves, Helichyrsum plicatum flowers, Solanum dulcamara aerial parts and Urtica dioica leaves gave the best inhibitory activity against S. pyogenes, S. aureus and S. epidermidis. Of the 22 plant extracts, 20 extracts displayed toxicity (LC50 was <1000 mg L(-1)) in the brine shrimp bioassay. For radish seed bioassay, two different determinations (root length and seed germination) were performed with a comparison between two concentrations (50,000 mg L(-1) and 10,000 mg L(-1)). At low concentration (10,000 mg L(-1)), S. dulcamara aerial parts and Primula vulgaris leaf extracts were observed to inhibit the root length more than the other plant extracts. Also, the most inhibitive plant extract for seed germination was obtained with S. dulcamara aerial parts.

Infrared film for aerial photography

USGS Publications Warehouse

Anderson, William H.

1979-01-01

Considerable interest has developed recently in the use of aerial photographs for agricultural management. Even the simplest hand-held aerial photographs, especially those taken with color infrared film, often provide information not ordinarily available through routine ground observation. When fields are viewed from above, patterns and variations become more apparent, often allowing problems to be spotted which otherwise may go undetected.

47 CFR 32.2421 - Aerial cable.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false Aerial cable. 32.2421 Section 32.2421 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES UNIFORM SYSTEM OF ACCOUNTS FOR TELECOMMUNICATIONS COMPANIES Instructions for Balance Sheet Accounts § 32.2421 Aerial cable. (a...

A workflow to preserve genome-quality tissue samples from plants in botanical gardens and arboreta1

PubMed Central

Gostel, Morgan R.; Kelloff, Carol; Wallick, Kyle; Funk, Vicki A.

2016-01-01

Premise of the study: Internationally, gardens hold diverse living collections that can be preserved for genomic research. Workflows have been developed for genomic tissue sampling in other taxa (e.g., vertebrates), but are inadequate for plants. We outline a workflow for tissue sampling intended for two audiences: botanists interested in genomics research and garden staff who plan to voucher living collections. Methods and Results: Standard herbarium methods are used to collect vouchers, label information and images are entered into a publicly accessible database, and leaf tissue is preserved in silica and liquid nitrogen. A five-step approach for genomic tissue sampling is presented for sampling from living collections according to current best practices. Conclusions: Collecting genome-quality samples from gardens is an economical and rapid way to make available for scientific research tissue from the diversity of plants on Earth. The Global Genome Initiative will facilitate and lead this endeavor through international partnerships. PMID:27672517

Behaviour of coconut mites preceding take-off to passive aerial dispersal.

PubMed

Melo, J W S; Lima, D B; Sabelis, M W; Pallini, A; Gondim, M G C

2014-12-01

For more than three decades the coconut mite Aceria guerreronis Keifer is one of the most important pests of coconut palms and has recently spread to many coconut production areas worldwide. Colonization of coconut palms is thought to arise from mites dispersing aerially after take-off from other plants within the same plantation or other plantations. The underlying dispersal behaviour of the mite at take-off, in the airborne state and after landing is largely unknown and this is essential to understand how they spread from tree to tree. In this article we studied whether take-off to aerial dispersal of coconut mites is preceded by characteristic behaviour, whether there is a correlation between the body position preceding aerial dispersal and the direction of the wind, and whether the substrate (outer surface of coconut bracts or epidermis) and the wind speed matter to the decision to take-off. We found that take-off can sometimes be preceded by a raised body stance, but more frequently take-off occurs while the mite is walking or resting on its substrate. Coconut mites that become airborne assumed a body stance that had no relation to the wind direction. Take-off was suppressed on a substrate providing food to coconut mites, but occurred significantly more frequently on the outer surface of coconut bracts than on the surface of the fruit. For both substrates, take-off frequency increased with wind speed. We conclude that coconut mites have at least some degree of control over take-off for aerial dispersal and that there is as yet no reason to infer that a raised body stance is necessary to become airborne.

Plant development. Integration of growth and patterning during vascular tissue formation in Arabidopsis.

PubMed

De Rybel, Bert; Adibi, Milad; Breda, Alice S; Wendrich, Jos R; Smit, Margot E; Novák, Ondřej; Yamaguchi, Nobutoshi; Yoshida, Saiko; Van Isterdael, Gert; Palovaara, Joakim; Nijsse, Bart; Boekschoten, Mark V; Hooiveld, Guido; Beeckman, Tom; Wagner, Doris; Ljung, Karin; Fleck, Christian; Weijers, Dolf

2014-08-08

Coordination of cell division and pattern formation is central to tissue and organ development, particularly in plants where walls prevent cell migration. Auxin and cytokinin are both critical for division and patterning, but it is unknown how these hormones converge upon tissue development. We identify a genetic network that reinforces an early embryonic bias in auxin distribution to create a local, nonresponding cytokinin source within the root vascular tissue. Experimental and theoretical evidence shows that these cells act as a tissue organizer by positioning the domain of oriented cell divisions. We further demonstrate that the auxin-cytokinin interaction acts as a spatial incoherent feed-forward loop, which is essential to generate distinct hormonal response zones, thus establishing a stable pattern within a growing vascular tissue. Copyright © 2014, American Association for the Advancement of Science.

Precision measurements from very-large scale aerial digital imagery.

PubMed

Booth, D Terrance; Cox, Samuel E; Berryman, Robert D

2006-01-01

Managers need measurements and resource managers need the length/width of a variety of items including that of animals, logs, streams, plant canopies, man-made objects, riparian habitat, vegetation patches and other things important in resource monitoring and land inspection. These types of measurements can now be easily and accurately obtained from very large scale aerial (VLSA) imagery having spatial resolutions as fine as 1 millimeter per pixel by using the three new software programs described here. VLSA images have small fields of view and are used for intermittent sampling across extensive landscapes. Pixel-coverage among images is influenced by small changes in airplane altitude above ground level (AGL) and orientation relative to the ground, as well as by changes in topography. These factors affect the object-to-camera distance used for image-resolution calculations. 'ImageMeasurement' offers a user-friendly interface for accounting for pixel-coverage variation among images by utilizing a database. 'LaserLOG' records and displays airplane altitude AGL measured from a high frequency laser rangefinder, and displays the vertical velocity. 'Merge' sorts through large amounts of data generated by LaserLOG and matches precise airplane altitudes with camera trigger times for input to the ImageMeasurement database. We discuss application of these tools, including error estimates. We found measurements from aerial images (collection resolution: 5-26 mm/pixel as projected on the ground) using ImageMeasurement, LaserLOG, and Merge, were accurate to centimeters with an error less than 10%. We recommend these software packages as a means for expanding the utility of aerial image data.

Interplays between Soil-Borne Plant Viruses and RNA Silencing-Mediated Antiviral Defense in Roots

PubMed Central

Andika, Ida Bagus; Kondo, Hideki; Sun, Liying

2016-01-01

Although the majority of plant viruses are transmitted by arthropod vectors and invade the host plants through the aerial parts, there is a considerable number of plant viruses that infect roots via soil-inhabiting vectors such as plasmodiophorids, chytrids, and nematodes. These soil-borne viruses belong to diverse families, and many of them cause serious diseases in major crop plants. Thus, roots are important organs for the life cycle of many viruses. Compared to shoots, roots have a distinct metabolism and particular physiological characteristics due to the differences in development, cell composition, gene expression patterns, and surrounding environmental conditions. RNA silencing is an important innate defense mechanism to combat virus infection in plants, but the specific information on the activities and molecular mechanism of RNA silencing-mediated viral defense in root tissue is still limited. In this review, we summarize and discuss the current knowledge regarding RNA silencing aspects of the interactions between soil-borne viruses and host plants. Overall, research evidence suggests that soil-borne viruses have evolved to adapt to the distinct mechanism of antiviral RNA silencing in roots. PMID:27695446

Looking for an old aerial photograph

USGS Publications Warehouse

,

1997-01-01

Attempts to photograph the surface of the Earth date from the 1800's, when photographers attached cameras to balloons, kites, and even pigeons. Today, aerial photographs and satellite images are commonplace. The rate of acquiring aerial photographs and satellite images has increased rapidly in recent years. Views of the Earth obtained from aircraft or satellites have become valuable tools to Government resource planners and managers, land-use experts, environmentalists, engineers, scientists, and a wide variety of other users. Many people want historical aerial photographs for business or personal reasons. They may want to locate the boundaries of an old farm or a piece of family property. Or they may want a photograph as a record of changes in their neighborhood, or as a gift. The U.S. Geological Survey (USGS) maintains the Earth Science Information Centers (ESIC?s) to sell aerial photographs, remotely sensed images from satellites, a wide array of digital geographic and cartographic data, as well as the Bureau?s wellknown maps. Declassified photographs from early spy satellites were recently added to the ESIC offerings of historical images. Using the Aerial Photography Summary Record System database, ESIC researchers can help customers find imagery in the collections of other Federal agencies and, in some cases, those of private companies that specialize in esoteric products.

Comparative analysis among the small RNA populations of source, sink and conductive tissues in two different plant-virus pathosystems.

PubMed

Herranz, Mari Carmen; Navarro, Jose Antonio; Sommen, Evelien; Pallas, Vicente

2015-02-22

In plants, RNA silencing plays a fundamental role as defence mechanism against viruses. During last years deep-sequencing technology has allowed to analyze the sRNA profile of a large variety of virus-infected tissues. Nevertheless, the majority of these studies have been restricted to a unique tissue and no comparative analysis between phloem and source/sink tissues has been conducted. In the present work, we compared the sRNA populations of source, sink and conductive (phloem) tissues in two different plant virus pathosystems. We chose two cucurbit species infected with two viruses very different in genome organization and replication strategy; Melon necrotic spot virus (MNSV) and Prunus necrotic ringspot virus (PNRSV). Our findings showed, in both systems, an increase of the 21-nt total sRNAs together with a decrease of those with a size of 24-nt in all the infected tissues, except for the phloem where the ratio of 21/24-nt sRNA species remained constant. Comparing the vsRNAs, both PNRSV- and MNSV-infected plants share the same vsRNA size distribution in all the analyzed tissues. Similar accumulation levels of sense and antisense vsRNAs were observed in both systems except for roots that showed a prevalence of (+) vsRNAs in both pathosystems. Additionally, the presence of overrepresented discrete sites along the viral genome, hot spots, were identified and validated by stem-loop RT-PCR. Despite that in PNRSV-infected plants the presence of vsRNAs was scarce both viruses modulated the host sRNA profile. We compare for the first time the sRNA profile of four different tissues, including source, sink and conductive (phloem) tissues, in two plant-virus pathosystems. Our results indicate that antiviral silencing machinery in melon and cucumber acts mainly through DCL4. Upon infection, the total sRNA pattern in phloem remains unchanged in contrast to the rest of the analyzed tissues indicating a certain tissue-tropism to this polulation. Independently of the

Nocturnal Accumulation of Malic Acid Occurs in Mesophyll Tissue without Proton Transport to Epidermal Tissue in the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum1

PubMed Central

Winter, Klaus; Edwards, Gerald E.; Holtum, Joseph A. M.

1981-01-01

The inducible Crassulacean acid metabolism plant, Mesembryanthemum crystallinum, accumulates malic acid, i.e. equivalent amounts of malate anions and protons in the mesophyll cells at night. Levels of malate and titratable acidity are low in the epidermal tissue and do not change significantly during the day/night cycle. This result is in contrast to a recent report (Bloom 1979 Plant Physiol 64: 919-923) that the synthesis of malic acid during dark CO2 fixation is associated with an equivalent exchange of inorganic cations from epidermal tissue with protons in the mesophyll cells. PMID:16661916

Aerial Refueling Boom/Receptacle Guide

DTIC Science & Technology

2017-07-28

Alleviation System; AR – Aerial Refueling; IDS – Independent Disconnect System; PDL – Pilot Director Lights; PSIG – Pounds per square inch gauge; TMF...proprietary, sensitive, classified or otherwise restricted information. ARSAG documents, as prepared, are not DOD, MOD or NATO standards, but provide...Boom Nozzle Disconnect Provisions, Aerial Refueling Fuel System and Tanker Aids and Cues for the Receiver Aircraft. Also included are Receiver

Aerial Photography

NASA Technical Reports Server (NTRS)

1985-01-01

John Hill, a pilot and commercial aerial photographer, needed an information base. He consulted NERAC and requested a search of the latest developments in camera optics. NERAC provided information; Hill contacted the manufacturers of camera equipment and reduced his photographic costs significantly.

Yield improvement strategies for the production of secondary metabolites in plant tissue culture: silymarin from Silybum marianum tissue culture.

PubMed

AbouZid, S

2014-01-01

Plant cell culture can be a potential source for the production of important secondary metabolites. This technology bears many advantages over conventional agricultural methods. The main problem to arrive at a cost-effective process is the low productivity. This is mainly due to lack of differentiation in the cultured cells. Many approaches have been used to maximise the yield of secondary metabolites produced by cultured plant cells. Among these approaches: choosing a plant with a high biosynthetic capacity, obtaining efficient cell line for growth and production of metabolite of interest, manipulating culture conditions, elicitation, metabolic engineering and organ culture. This article gives an overview of the various approaches used to maximise the production of pharmaceutically important secondary metabolites in plant cell cultures. Examples of using these different approaches are shown for the production of silymarin from Silybum marianum tissue culture.

Fungal endophytes in above-ground tissues of desert plants: infrequent in culture, but highly diverse and distinctive symbionts

PubMed Central

Massimo, Nicholas C.; Nandi Devan, MM; Arendt, Kayla R.; Wilch, Margaret H.; Riddle, Jakob M.; Furr, Susan H.; Steen, Cole; U'Ren, Jana M.; Sandberg, Dustin C.; Arnold, A. Elizabeth

2015-01-01

In hot deserts, plants cope with aridity, high temperatures, and nutrient-poor soils with morphological and biochemical adaptations that encompass intimate microbial symbioses. Whereas the root microbiomes of arid-land plants have received increasing attention, factors influencing assemblages of symbionts in above-ground tissues have not been evaluated for many woody plants that flourish in desert environments. We evaluated the diversity, host affiliations, and distributions of endophytic fungi associated with photosynthetic tissues of desert trees and shrubs, focusing on non-succulent woody plants in the species-rich Sonoran Desert. To inform our strength of inference, we evaluated the effects of two different nutrient media, incubation temperatures, and collection seasons on the apparent structure of endophyte assemblages. Analysis of >22,000 tissue segments revealed that endophytes were isolated four times more frequently from photosynthetic stems than leaves. Isolation frequency was lower than expected given the latitude of the study region, and varied among species a function of sampling site and abiotic factors. However, endophytes were very species-rich and phylogenetically diverse, consistent with less-arid sites of a similar latitudinal position. Community composition differed among host species, but not as a function of tissue type, sampling site, sampling month, or exposure. Estimates of abundance, diversity and composition were not influenced by isolation medium or incubation temperature. Phylogenetic analyses of the most commonly isolated genus (Preussia) revealed multiple evolutionary origins of desert-plant endophytism and little phylogenetic structure with regard to seasonality, tissue preference, or optimal temperatures and nutrients for growth in vitro. Together, these results provide insight into endophytic symbioses in desert plant communities, and can be used to optimize strategies for capturing endophyte biodiversity at regional scales. PMID

Draper Laboratory small autonomous aerial vehicle

NASA Astrophysics Data System (ADS)

DeBitetto, Paul A.; Johnson, Eric N.; Bosse, Michael C.; Trott, Christian A.

1997-06-01

The Charles Stark Draper Laboratory, Inc. and students from Massachusetts Institute of Technology and Boston University have cooperated to develop an autonomous aerial vehicle that won the 1996 International Aerial Robotics Competition. This paper describes the approach, system architecture and subsystem designs for the entry. This entry represents a combination of many technology areas: navigation, guidance, control, vision processing, human factors, packaging, power, real-time software, and others. The aerial vehicle, an autonomous helicopter, performs navigation and control functions using multiple sensors: differential GPS, inertial measurement unit, sonar altimeter, and a flux compass. The aerial transmits video imagery to the ground. A ground based vision processor converts the image data into target position and classification estimates. The system was designed, built, and flown in less than one year and has provided many lessons about autonomous vehicle systems, several of which are discussed. In an appendix, our current research in augmenting the navigation system with vision- based estimates is presented.

47 CFR 32.2431 - Aerial wire.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false Aerial wire. 32.2431 Section 32.2431... FOR TELECOMMUNICATIONS COMPANIES Instructions for Balance Sheet Accounts § 32.2431 Aerial wire. (a) This account shall include the original cost of bare line wire and other material used in the...

47 CFR 32.2431 - Aerial wire.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 2 2011-10-01 2011-10-01 false Aerial wire. 32.2431 Section 32.2431... FOR TELECOMMUNICATIONS COMPANIES Instructions for Balance Sheet Accounts § 32.2431 Aerial wire. (a) This account shall include the original cost of bare line wire and other material used in the...

[Chemical constituents from the aerial part of Stauntonia obovatifoliola Hayata subsp. urophylla].

PubMed

Peng, Xiao-bing; Gao, Wei-lue; Hu, Dong-qun; Ma, Fang-fang; Fu, Li-gang; Deng, Qian; Wei, Ying

2013-11-01

To study the chemical constituents of the aerial part of Stauntonia obovatifoliola. The chemical constituents of ethyl acetate fraction were isolated and purified by several chromatography. Their structures were elucidated by their physiochemical properties and spectral methods. Six known compounds were isolated and identified as lupeone(1), lupeol(2), stigmasterol(3),3beta-O-acetyloleanolic acid(4), resinone(5) and daucosterol(6). Compounds 1-6 are isolated from this plant for the first time.

Very high resolution aerial films

NASA Astrophysics Data System (ADS)

Becker, Rolf

1986-11-01

The use of very high resolution aerial films in aerial photography is evaluated. Commonly used panchromatic, color, and CIR films and their high resolution equivalents are compared. Based on practical experience and systematic investigations, the very high image quality and improved height accuracy that can be achieved using these films are demonstrated. Advantages to be gained from this improvement and operational restrictions encountered when using high resolution film are discussed.

Summary of Investigations of Mark 25 Aerial-Torpedo Turbine

NASA Technical Reports Server (NTRS)

Schum, Harold J.; Whitney, Warren J.; Buckner, Howard A., Jr.

1950-01-01

The power plant from a Mark 25 aerial torpedo was investigated both as a two-stage turbine and as a single-stage modified turbine to determine the effect on overall performance of nozzle size and shape, first-stage rotor-blade configuration, and axial nozzle-rotor running clearance. Performance was evaluated in terms of brake, rotor, and blade efficiencies. All the performance data were obtained for inlet total to outlet static pressure ratios of 8, 15 (design), and 20 with inlet conditions maintained constant at 95 pounds per square inch gage and 1000 F for rotor speeds from approximately 6000 to 18,000 rpm.

Assessment of a 1H high-resolution magic angle spinning NMR spectroscopy procedure for free sugars quantification in intact plant tissue.

PubMed

Delgado-Goñi, Teresa; Campo, Sonia; Martín-Sitjar, Juana; Cabañas, Miquel E; San Segundo, Blanca; Arús, Carles

2013-08-01

In most plants, sucrose is the primary product of photosynthesis, the transport form of assimilated carbon, and also one of the main factors determining sweetness in fresh fruits. Traditional methods for sugar quantification (mainly sucrose, glucose and fructose) require obtaining crude plant extracts, which sometimes involve substantial sample manipulation, making the process time-consuming and increasing the risk of sample degradation. Here, we describe and validate a fast method to determine sugar content in intact plant tissue by using high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR). The HR-MAS NMR method was used for quantifying sucrose, glucose and fructose in mesocarp tissues from melon fruits (Cucumis melo var. reticulatus and Cucumis melo var. cantalupensis). The resulting sugar content varied among individual melons, ranging from 1.4 to 7.3 g of sucrose, 0.4-2.5 g of glucose; and 0.73-2.83 g of fructose (values per 100 g fw). These values were in agreement with those described in the literature for melon fruit tissue, and no significant differences were found when comparing them with those obtained using the traditional, enzymatic procedure, on melon tissue extracts. The HR-MAS NMR method offers a fast (usually <30 min) and sensitive method for sugar quantification in intact plant tissues, it requires a small amount of tissue (typically 50 mg fw) and avoids the interferences and risks associated with obtaining plant extracts. Furthermore, this method might also allow the quantification of additional metabolites detectable in the plant tissue NMR spectrum.

24. AERIAL VIEW LOOKING SOUTHEAST AT BUILDING 371 UNDER CONSTRUCTION ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. AERIAL VIEW LOOKING SOUTHEAST AT BUILDING 371 UNDER CONSTRUCTION IN 1974. BY 1968, BUILDING 771 WAS OUTMODED AND NEW TECHNOLOGIES HAD BEEN DEVELOPED FOR PLUTONIUM RECOVERY. AS A RESULT, A NEW RECOVERY BUILDING, BUILDING 371 WAS PLANNED. BUILDING 371 SUFFERED FROM VARIOUS DESIGN PROBLEMS, WHICH PREVENTED ITS OPENING UNTIL 1981 AND CAUSED TERMINATION OF RECOVERY OPERATIONS IN 1986. IT NEVER BECAME FULLY OPERATIONAL. TO THE EAST OF BUILDING 371, IS THE 700 BUILDING COMPLEX (4/74). - Rocky Flats Plant, Bounded by Indiana Street & Routes 93, 128 & 72, Golden, Jefferson County, CO

Identification or Development of Chemical Analysis Methods for Plants and Animal Tissues

DTIC Science & Technology

1981-01-01

Report No. DRXTH-TE-CR-80086 [E EMWSTEEACISITE - IDENTIFICATION OR DEVELOPMENT OF CHEMICAL ANALYSISI METHODS FOR PLANTS AND ANIMAL TISSUES D l...86i 4TITLE (ansdo"t) TYPE or" P 4. Iih~iti.)Final epwt. )9 A4,417 ~, Identlifiation or Development of Chemical I-g 9 O d 18 Analysis Methods for Plants...n TN oT sacmpihdo in. wEr DContinng er adetcsr aid Iuent bybopkmertda lo aeo 1.5th 1/s deeipta24ndectr Tritrotasolsoepae d andT Boloia Matrc,, 1473

Extraction of high-quality DNA from ethanol-preserved tropical plant tissues.

PubMed

Bressan, Eduardo A; Rossi, Mônica L; Gerald, Lee T S; Figueira, Antonio

2014-04-24

Proper conservation of plant samples, especially during remote field collection, is essential to assure quality of extracted DNA. Tropical plant species contain considerable amounts of secondary compounds, such as polysaccharides, phenols, and latex, which affect DNA quality during extraction. The suitability of ethanol (96% v/v) as a preservative solution prior to DNA extraction was evaluated using leaves of Jatropha curcas and other tropical species. Total DNA extracted from leaf samples stored in liquid nitrogen or ethanol from J. curcas and other tropical species (Theobroma cacao, Coffea arabica, Ricinus communis, Saccharum spp., and Solanum lycopersicon) was similar in quality, with high-molecular-weight DNA visualized by gel electrophoresis. DNA quality was confirmed by digestion with EcoRI or HindIII and by amplification of the ribosomal gene internal transcribed spacer region. Leaf tissue of J. curcas was analyzed by light and transmission electron microscopy before and after exposure to ethanol. Our results indicate that leaf samples can be successfully preserved in ethanol for long periods (30 days) as a viable method for fixation and conservation of DNA from leaves. The success of this technique is likely due to reduction or inactivation of secondary metabolites that could contaminate or degrade genomic DNA. Tissue conservation in 96% ethanol represents an attractive low-cost alternative to commonly used methods for preservation of samples for DNA extraction. This technique yields DNA of equivalent quality to that obtained from fresh or frozen tissue.

Extraction of high-quality DNA from ethanol-preserved tropical plant tissues

PubMed Central

2014-01-01

Background Proper conservation of plant samples, especially during remote field collection, is essential to assure quality of extracted DNA. Tropical plant species contain considerable amounts of secondary compounds, such as polysaccharides, phenols, and latex, which affect DNA quality during extraction. The suitability of ethanol (96% v/v) as a preservative solution prior to DNA extraction was evaluated using leaves of Jatropha curcas and other tropical species. Results Total DNA extracted from leaf samples stored in liquid nitrogen or ethanol from J. curcas and other tropical species (Theobroma cacao, Coffea arabica, Ricinus communis, Saccharum spp., and Solanum lycopersicon) was similar in quality, with high-molecular-weight DNA visualized by gel electrophoresis. DNA quality was confirmed by digestion with EcoRI or HindIII and by amplification of the ribosomal gene internal transcribed spacer region. Leaf tissue of J. curcas was analyzed by light and transmission electron microscopy before and after exposure to ethanol. Our results indicate that leaf samples can be successfully preserved in ethanol for long periods (30 days) as a viable method for fixation and conservation of DNA from leaves. The success of this technique is likely due to reduction or inactivation of secondary metabolites that could contaminate or degrade genomic DNA. Conclusions Tissue conservation in 96% ethanol represents an attractive low-cost alternative to commonly used methods for preservation of samples for DNA extraction. This technique yields DNA of equivalent quality to that obtained from fresh or frozen tissue. PMID:24761774

An optical clearing technique for plant tissues allowing deep imaging and compatible with fluorescence microscopy.

PubMed

Warner, Cherish A; Biedrzycki, Meredith L; Jacobs, Samuel S; Wisser, Randall J; Caplan, Jeffrey L; Sherrier, D Janine

2014-12-01

We report on a nondestructive clearing technique that enhances transmission of light through specimens from diverse plant species, opening unique opportunities for microscope-enabled plant research. After clearing, plant organs and thick tissue sections are amenable to deep imaging. The clearing method is compatible with immunocytochemistry techniques and can be used in concert with common fluorescent probes, including widely adopted protein tags such as GFP, which has fluorescence that is preserved during the clearing process. © 2014 American Society of Plant Biologists. All Rights Reserved.

Results of laboratory testing for diphacinone in seawater, fish, invertebrates, and soil following aerial application of rodenticide on Lehua Island, Kauai County, Hawaii, January 2009

USGS Publications Warehouse

Orazio, Carl E.; Tanner, Michael J.; Swenson, Chris; Herod, Jeffrey J.; Dunlevy, Peter; Gale, Robert W.

2009-01-01

In January 2009, rodenticide bait (Ramik Green pellets) containing the active ingredient diphacinone was aerially applied to Lehua Island. Reported herein are the results of laboratory analyses to determine diphacinone concentrations in samples of seawater, fillet of fish, soft tissue of limpets (opihi), whole-body crabs, and soil collected from Lehua Island, Kauai County, Hawaii, after aerial application of the rodenticide bait. Diphacinone was specifically chosen because of its low toxicity to nontarget organisms. Its use on Lehua Island is the second time it has ever been used for an aerial application to eradicate rodents. Testing of the Lehua Island samples for diphacinone utilized high-performance liquid chromatography with photodiode array detection. No detectable concentrations of diphacinone were found in any of the samples from Lehua Island. The limits of detection for diphacinone were 0.4 nanograms per milliliter (parts per billion) seawater, 15 nanograms per gram (dry weight) soil, 20 nanograms per gram (parts per billion) fish fillet, 13 nanograms per gram whole crab, and 34 nanograms per gram soft tissue limpet.

In vivo monitoring of seeds and plant-tissue water absorption using optical coherence tomography and optical coherence microscopy

NASA Astrophysics Data System (ADS)

Sapozhnikova, Veronika V.; Kutis, Irina S.; Kutis, Sergey D.; Kuranov, Roman V.; Gelikonov, Grigory V.; Shabanov, Dmitry V.; Kamensky, Vladislav A.

2004-07-01

First experimental results on OCT imaging of internal structure of plant tissues and in situ OCT monitoring of plant tissue regeneration at different water supply are reported. Experiments for evaluating OCT capabilities were performed on Tradescantia. The investigation of seeds swelling was performed on wheat seeds (Triticum L.), barley seeds (Hordeum L.), long-fibred flax seeds (Linum usitatissimum L.) and cucumber seeds (Cucumis sativus L.). These OCT images correlate with standard microscopy data from the same tissue regions. Seeds were exposed to a low-intensity physical factor-the pulsed gradient magnetic field (GMF) with pulse duration 0.1 s and maximum amplitude 5 mT (4 successive pulses during 0.4 s). OCT and OCM enable effective monitoring of fast reactions in plants and seeds at different water supply.

EFFECT OF AMBIENT LIGHT, AERIAL EXPOSURE, AND SEASON ON EELGRASS (ZOSTERA MARINA) METRICS IN A NORTHEAST PACIFIC (USA) ESTUARY

EPA Science Inventory

Although light is the principal factor controlling the lower depth limit of seagrasses, little attention has been given to how reduced winter lighting may affect intertidal plants. In the present study intertidal light intensity, temperature, and aerial exposure were measured ove...

Waterlogging tolerance, tissue nitrogen and oxygen transport in the forage legume Melilotus siculus: a comparison of nodulated and nitrate-fed plants.

PubMed

Konnerup, Dennis; Toro, Guillermo; Pedersen, Ole; Colmer, Timothy David

2018-03-14

Soil waterlogging adversely impacts most plants. Melilotus siculus is a waterlogging-tolerant annual forage legume, but data were lacking for the effects of root-zone hypoxia on nodulated plants reliant on N2 fixation. The aim was to compare the waterlogging tolerance and physiology of M. siculus reliant on N2 fixation or with access to NO3-. A factorial experiment imposed treatments of water level (drained or waterlogged), rhizobia (nil or inoculated) and mineral N supply (nil or 11 mm NO3-) for 21 d on plants in pots of vermiculite in a glasshouse. Nodulation, shoot and root growth and tissue N were determined. Porosity (gas volume per unit tissue volume) and respiration rates of root tissues and nodules, and O2 microelectrode profiling across nodules, were measured in a second experiment. Plants inoculated with the appropriate rhizobia, Ensifer (syn. Sinorhizobium) medicae, formed nodules. Nodulated plants grew as well as plants fed NO3-, both in drained and waterlogged conditions. The growth and total N content of nodulated plants (without any NO3- supplied) indicated N2 fixation. Respiration rates (mass basis) were highest in nodules and root tips and lowest in basal root tissues. Secondary aerenchyma (phellem) formed along basal root parts and a thin layer of this porous tissue also covered nodules, which together enhanced gas-phase diffusion of O2 to the nodules; O2 was below detection within the infected zone of the nodule interior. Melilotus siculus reliant on N2 fixation grew well both in drained and waterlogged conditions, and had similar tissue N concentrations. In waterlogged conditions the relatively high respiration rates of nodules must rely on O2 movement via the aerenchymatous phellem in hypocotyl, roots and the outer tissue layers of nodules.

Universal Algorithms for Plant Phenotyping: Are we there yet?

NASA Astrophysics Data System (ADS)

Kakani, V. G.; Kambham, R. R.; Zhao, D.; Foster, A. J.; Gowda, P. H.

2017-12-01

Hyperspectral remote sensing offers ability to capture spectral signatures of plant morpho-physio-biochemical traits at multiple scales (leaf to canopy to aerial). Experimental results on plant phenotype from pot, growth chamber and field studies at multiple location were used in this study. Pigment, leaf/plant water status, plant nutrient status, plant height, leaf area, fresh and dry weights of biomass and its components are correlated with hyperspectral reflectance signatures. Leaf reflectance was collected with spectroradiometer having a light source. Canopy hyperspectral reflectance was collected from 1.5 m above the canopy using a spectroradiometer, while multispectral images were acquired from aerial platforms ( 400m). Several statistical methods including simple ratios, principal component analysis, and partial least squares regression were used to identify hyperspectral reflectance bands that were tightly associated with plant phenotypic traits. Leaf level spectra best described the morpho-physio-biochemical traits (R2 = 0.6-0.9), while canopy reflectance best described plant height (R2 = 0.65), leaf area index (R2 = 0.67-0.74) and biomass (R2 = 0.69-0.78), while aerial spectra improved canopy level regression coefficients for plant height (R2 = 0.93) and leaf area index (R2 = 0.89). The comparison of multi-level spectra and resolution, clearly showed the advantage of hyperspectral reflectance data over the multispectral reflectance data, particularly for understanding the basis for spectral reflectance differences among species and traits. In conclusion, high resolution (1-2 cm) spectral imagery can help to bridge the gap across multiple levels of phenotype measurement.

22. AERIAL VIEW LOOKING EAST DOWN CENTRAL AVENUE FROM WEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. AERIAL VIEW LOOKING EAST DOWN CENTRAL AVENUE FROM WEST OF THE ADMINISTRATIVE AND SUPPORT AREA OF THE PLANT. ON THE LEFT (NORTH) SIDE OF THE STREET IN THE FOREGROUND OF THE PHOTOGRAPH IS BUILDING 111, THE GENERAL ADMINISTRATION BUILDING. TO THE EAST OF BUILDING 111 IS BUILDING 112, THE CAFETERIA. FURTHER TO THE EAST IS BUILDING 331, THE VEHICLE MAINTENANCE GARAGE AND FIRE DEPARTMENT; BUILDING 333, THE PAINT SHOP; BUILDING 334, THE ELECTRICAL AND GENERAL MAINTENANCE SHOP; AND BUILDING 551, THE GENERAL WAREHOUSE. ON THE RIGHT (SOUTH) SIDE OF CENTRAL AVENUE, IN THE FOREGROUND IS BUILDING 121, FIREARMS REPAIR. BEHIND BUILDING 121 IS BUILDING 122, EMERGENCY MEDICAL SERVICES, AND BUILDING 123, HEALTH PHYSICS LABORATORY. BUILDING 441, THE PRODUCTION ... - Rocky Flats Plant, Bounded by Indiana Street & Routes 93, 128 & 72, Golden, Jefferson County, CO

Effect of x-ray irradiation on maize inbred line B73 tissue cultures and regenerated plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, A.S.; Cheng, D.S.K.; Milcic, J.B.

In order to enhance variation induced by the tissue culture process and to obtain agronomically desirable mutants, friable embryogenic tissue cultures of maize (Zea mays L.) inbred line B73 were x-ray irradiated with 11 doses (0-8.4 kilorads (kR)). Reductions in callus growth rate and embryogenic callus formation occurred with increasing x-ray doses 20 d and 3 months after irradiation. Callus irradiated with 0.8 kR showed a significant increase in growth rate and a 20% increase in embryogenic callus 9 months after irradiation. A total of 230 R/sub 0/ plants were regenerated for evaluation. Pollen fertility and seed set of R/submore » 0/ plants decreased with increasing x-ray dosage. Days to anthesis and plant height of R/sub 0/ plants varied among x-ray treatments but were generally reduced with higher dosages. The number of chromosomal aberrations increased with x-ray dosage. The R/sub 1/ seeds taken from R/sub 0/ plants were also grown and tested for mutant segregation. Plants regenerated from irradiated calli had a two- to 10-fold increase in mutations over plants regenerated from unirradiated control callus. Germination frequency of seeds from R/sub 0/ plants decreased with increasing x-ray dosage. Although chlorophyll mutants were most frequently observed, a number of vigorous plants with earlier anthesis date were also recovered.« less

Nitrate dynamics in natural plants: insights based on the concentration and natural isotope abundances of tissue nitrate

PubMed Central

Liu, Xue-Yan; Koba, Keisuke; Makabe, Akiko; Liu, Cong-Qiang

2014-01-01

The dynamics of nitrate (NO−3), a major nitrogen (N) source for natural plants, has been studied mostly through experimental N addition, enzymatic assay, isotope labeling, and genetic expression. However, artificial N supply may not reasonably reflect the N strategies in natural plants because NO−3 uptake and reduction may vary with external N availability. Due to abrupt application and short operation time, field N addition, and isotopic labeling hinder the elucidation of in situ NO−3-use mechanisms. The concentration and natural isotopes of tissue NO−3 can offer insights into the plant NO−3 sources and dynamics in a natural context. Furthermore, they facilitate the exploration of plant NO−3 utilization and its interaction with N pollution and ecosystem N cycles without disturbing the N pools. The present study was conducted to review the application of the denitrifier method for concentration and isotope analyses of NO−3 in plants. Moreover, this study highlights the utility and advantages of these parameters in interpreting NO−3 sources and dynamics in natural plants. We summarize the major sources and reduction processes of NO−3 in plants, and discuss the implications of NO−3 concentration in plant tissues based on existing data. Particular emphasis was laid on the regulation of soil NO−3 and plant ecophysiological functions in interspecific and intra-plant NO−3 variations. We introduce N and O isotope systematics of NO−3 in plants and discuss the principles and feasibilities of using isotopic enrichment and fractionation factors; the correlation between concentration and isotopes (N and O isotopes: δ18O and Δ17O); and isotope mass-balance calculations to constrain sources and reduction of NO−3 in possible scenarios for natural plants are deliberated. Finally, we offer a preliminary framework of intraplant δ18O-NO−3 variation, and summarize the uncertainties in using tissue NO−3 parameters to interpret plant NO−3 utilization

Nitrate dynamics in natural plants: insights based on the concentration and natural isotope abundances of tissue nitrate.

PubMed

Liu, Xue-Yan; Koba, Keisuke; Makabe, Akiko; Liu, Cong-Qiang

2014-01-01

The dynamics of nitrate (NO(-) 3), a major nitrogen (N) source for natural plants, has been studied mostly through experimental N addition, enzymatic assay, isotope labeling, and genetic expression. However, artificial N supply may not reasonably reflect the N strategies in natural plants because NO(-) 3 uptake and reduction may vary with external N availability. Due to abrupt application and short operation time, field N addition, and isotopic labeling hinder the elucidation of in situ NO(-) 3-use mechanisms. The concentration and natural isotopes of tissue NO(-) 3 can offer insights into the plant NO(-) 3 sources and dynamics in a natural context. Furthermore, they facilitate the exploration of plant NO(-) 3 utilization and its interaction with N pollution and ecosystem N cycles without disturbing the N pools. The present study was conducted to review the application of the denitrifier method for concentration and isotope analyses of NO(-) 3 in plants. Moreover, this study highlights the utility and advantages of these parameters in interpreting NO(-) 3 sources and dynamics in natural plants. We summarize the major sources and reduction processes of NO(-) 3 in plants, and discuss the implications of NO(-) 3 concentration in plant tissues based on existing data. Particular emphasis was laid on the regulation of soil NO(-) 3 and plant ecophysiological functions in interspecific and intra-plant NO(-) 3 variations. We introduce N and O isotope systematics of NO(-) 3 in plants and discuss the principles and feasibilities of using isotopic enrichment and fractionation factors; the correlation between concentration and isotopes (N and O isotopes: δ(18)O and Δ(17)O); and isotope mass-balance calculations to constrain sources and reduction of NO(-) 3 in possible scenarios for natural plants are deliberated. Finally, we offer a preliminary framework of intraplant δ(18)O-NO(-) 3 variation, and summarize the uncertainties in using tissue NO(-) 3 parameters to interpret

Unmanned aerial systems for forest reclamation monitoring: throwing balloons in the air

NASA Astrophysics Data System (ADS)

Andrade, Rita; Vaz, Eric; Panagopoulos, Thomas; Guerrero, Carlos

2014-05-01

Wildfires are a recurrent phenomenon in Mediterranean landscapes, deteriorating environment and ecosystems, calling out for adequate land management. Monitoring burned areas enhances our abilities to reclaim them. Remote sensing has become an increasingly important tool for environmental assessment and land management. It is fast, non-intrusive, and provides continuous spatial coverage. This paper reviews remote sensing methods, based on space-borne, airborne or ground-based multispectral imagery, for monitoring the biophysical properties of forest areas for site specific management. The usage of satellite imagery for land use management has been frequent in the last decades, it is of great use to determine plants health and crop conditions, allowing a synergy between the complexity of environment, anthropogenic landscapes and multi-temporal understanding of spatial dynamics. Aerial photography increments on spatial resolution, nevertheless it is heavily dependent on airborne availability as well as cost. Both these methods are required for wide areas management and policy planning. Comprising an active and high resolution imagery source, that can be brought at a specific instance, reducing cost while maintaining locational flexibility is of utmost importance for local management. In this sense, unmanned aerial vehicles provide maximum flexibility with image collection, they can incorporate thermal and multispectral sensors, however payload and engine operation time limit flight time. Balloon remote sensing is becoming increasingly sought after for site specific management, catering rapid digital analysis, permitting greater control of the spatial resolution as well as of datasets collection in a given time. Different wavelength sensors may be used to map spectral variations in plant growth, monitor water and nutrient stress, assess yield and plant vitality during different stages of development. Proximity could be an asset when monitoring forest plants vitality

Lichens as bioindicators of aerial fallout of heavy metals in Zaria, Nigeria

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kapu, M.M.; Ipaye, M.M.; Ega, R.A.I.

1991-09-01

Lichens and other epiphytic cryptogams possess efficient ion-exchange mechanisms which enable many species to accumulate airborne metals and which probably contribute to their tolerating metals at concentrations high enough to cause death to other plant species. A direct relationship between the distribution pattern of lichens and the trace metal content of the surrounding air has been demonstrated. The present study used lichens to assess the aerial fallout of heavy metals from traffic in Zaria, northern Nigeria.

Multiscale Investigation from Subcellular to Tissue Scale of Onion Epidermal Plant Cell Wall Mechanical Properties

NASA Astrophysics Data System (ADS)

Zamil, Mohammad Shafayet

The physical and mechanical properties of cell walls, their shape, how they are arranged and interact with each other determine the architecture of plant organs and how they mechanically respond to different environmental and loading conditions. Due to the distinctive hierarchy from subcellular to tissue scale, plant materials can exhibit remarkably different mechanical properties. To date, how the subcellular scale arrangement and the mechanical properties of plant cell wall structural constituents give rise to macro or tissue scale mechanical responses is not yet well understood. Although the tissue scale plant cell wall samples are easy to prepare and put to different types of mechanical tests, the hierarchical features that emerge when moving towards a higher scale make it complicated to link the macro scale results to micro or subcellular scale structural components. On the other hand, the microscale size of cell brings formidable challenges to prepare and grip samples and carry mechanical tests under tensile loading at subcellular scale. This study attempted to develop a set of test protocols based on microelectromechanical system (MEMS) tensile testing devices for characterizing plant cell wall materials at different length scales. For the ease of sample preparation and well established database of the composition and conformation of its structural constituents, onion epidermal cell wall profile was chosen as the study material. Based on the results and findings of multiscale mechanical characterization, a framework of architecture-based finite element method (FEM) computational model was developed. The computational model laid the foundation of bridging the subcellular or microscale to the tissue or macroscale mechanical properties. This study suggests that there are important insights of cell wall mechanics and structural features that can only be investigated by carrying tensile characterization of samples not confounded by extracellular parameters. To

Simple preparation of plant epidermal tissue for laser microdissection and downstream quantitative proteome and carbohydrate analysis.

PubMed

Falter, Christian; Ellinger, Dorothea; von Hülsen, Behrend; Heim, René; Voigt, Christian A

2015-01-01

The outwardly directed cell wall and associated plasma membrane of epidermal cells represent the first layers of plant defense against intruding pathogens. Cell wall modifications and the formation of defense structures at sites of attempted pathogen penetration are decisive for plant defense. A precise isolation of these stress-induced structures would allow a specific analysis of regulatory mechanism and cell wall adaption. However, methods for large-scale epidermal tissue preparation from the model plant Arabidopsis thaliana, which would allow proteome and cell wall analysis of complete, laser-microdissected epidermal defense structures, have not been provided. We developed the adhesive tape - liquid cover glass technique (ACT) for simple leaf epidermis preparation from A. thaliana, which is also applicable on grass leaves. This method is compatible with subsequent staining techniques to visualize stress-related cell wall structures, which were precisely isolated from the epidermal tissue layer by laser microdissection (LM) coupled to laser pressure catapulting. We successfully demonstrated that these specific epidermal tissue samples could be used for quantitative downstream proteome and cell wall analysis. The development of the ACT for simple leaf epidermis preparation and the compatibility to LM and downstream quantitative analysis opens new possibilities in the precise examination of stress- and pathogen-related cell wall structures in epidermal cells. Because the developed tissue processing is also applicable on A. thaliana, well-established, model pathosystems that include the interaction with powdery mildews can be studied to determine principal regulatory mechanisms in plant-microbe interaction with their potential outreach into crop breeding.

3D Reconstruction of Frozen Plant Tissue: a unique histological analysis to image post-freeze responses

USDA-ARS?s Scientific Manuscript database

Winter hardiness in plants is the result of a complex interaction between genes, the tissue where those genes are expressed and the environment. The light microscope is a valuable tool to understand this complexity which will ultimately help researchers improve the tolerance of plants to freezing st...

Development of an improved sample preparation platform for acidic endogenous hormones in plant tissues using electromembrane extraction.

PubMed

Suh, Joon Hyuk; Han, Sang Beom; Wang, Yu

2018-02-02

Despite their importance in pivotal signaling pathways due to trace quantities and complex matrices, the analysis of plant hormones is a challenge. Here, to improve this issue, we present an electromembrane extraction technology combined with liquid chromatography-tandem mass spectrometry for determination of acidic plant hormones including jasmonic acid, abscisic acid, salicylic acid, benzoic acid, gibberellic acid and gibberellin A 4 in plant tissues. Factors influencing extraction efficiency, such as voltage, extraction time and stirring rate were optimized using a design of experiments. Analytical performance was evaluated in terms of specificity, linearity, limit of quantification, precision, accuracy, recovery and repeatability. The results showed good linearity (r 2  > 0.995), precision and acceptable accuracy. The limit of quantification ranged from 0.1 to 10 ng mL -1 , and the recoveries were 34.6-50.3%. The developed method was applied in citrus leaf samples, showing better clean-up efficiency, as well as higher sensitivity compared to a previous method using liquid-liquid extraction. Organic solvent consumption was minimized during the process, making it an appealing method. More noteworthy, electromembrane extraction has been scarcely applied to plant tissues, and this is the first time that major plant hormones were extracted using this technology, with high sensitivity and selectivity. Taken together, this work gives not only a novel sample preparation platform using an electric field for plant hormones, but also a good example of extracting complex plant tissues in a simple and effective way. Copyright © 2017 Elsevier B.V. All rights reserved.

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

PubMed Central

Bobik, Krzysztof; Dunlap, John R.; Burch-Smith, Tessa M.

2014-01-01

Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, because of laborious and time-consuming protocols that also demand experience in preparation of artifact-free samples, TEM is not considered a user-friendly technique. Traditional sample preparation for TEM used chemical fixatives to preserve cellular structures. High-pressure freezing is the cryofixation of biological samples under high pressures to produce very fast cooling rates, thereby restricting ice formation, which is detrimental to the integrity of cellular ultrastructure. High-pressure freezing and freeze substitution are currently the methods of choice for producing the highest quality morphology in resin sections for TEM. These methods minimize the artifacts normally associated with conventional processing for TEM of thin sections. After cryofixation the frozen water in the sample is replaced with liquid organic solvent at low temperatures, a process called freeze substitution. Freeze substitution is typically carried out over several days in dedicated, costly equipment. A recent innovation allows the process to be completed in three hours, instead of the usual two days. This is typically followed by several more days of sample preparation that includes infiltration and embedding in epoxy resins before sectioning. Here we present a protocol combining high-pressure freezing and quick freeze substitution that enables plant sample fixation to be accomplished within hours. The protocol can readily be adapted for working with other tissues or organisms. Plant tissues are of special concern because of the presence of aerated spaces and water-filled vacuoles that impede ice-free freezing of water. In addition, the process of chemical fixation is especially long in plants due to cell walls impeding the penetration of the chemicals to deep within the tissues. Plant tissues are therefore particularly challenging, but

Dark respiration rate increases with plant size in saplings of three temperate tree species despite decreasing tissue nitrogen and nonstructural carbohydrates.

PubMed

Machado, José-Luis; Reich, Peter B

2006-07-01

In shaded environments, minimizing dark respiration during growth could be an important aspect of maintaining a positive whole-plant net carbon balance. Changes with plant size in both biomass distribution to different tissue types and mass-specific respiration rates (R(d)) of those tissues would have an impact on whole-plant respiration. In this paper, we evaluated size-related variation in R(d), biomass distribution, and nitrogen (N) and total nonstructural carbohydrate (TNC) concentrations of leaves, stems and roots of three cold-temperate tree species (Abies balsamea (L.) Mill, Acer rubrum L. and Pinus strobus L.) in a forest understory. We sampled individuals varying in age (6 to 24 years old) and in size (from 2 to 500 g dry mass), and growing across a range of irradiances (from 1 to 13% of full sun) in northern Minnesota, USA. Within each species, we found small changes in R(d), N and TNC when comparing plants growing across this range of light availability. Consistent with our hypotheses, as plants grew larger, whole-plant N and TNC concentrations in all species declined as a result of a combination of changes in tissue N and shifts in biomass distribution patterns. However, contrary to our hypotheses, whole-plant and tissue R(d) increased with plant size in the three species.

Estimation of the solubility parameters of model plant surfaces and agrochemicals: a valuable tool for understanding plant surface interactions

PubMed Central

2012-01-01

Background Most aerial plant parts are covered with a hydrophobic lipid-rich cuticle, which is the interface between the plant organs and the surrounding environment. Plant surfaces may have a high degree of hydrophobicity because of the combined effects of surface chemistry and roughness. The physical and chemical complexity of the plant cuticle limits the development of models that explain its internal structure and interactions with surface-applied agrochemicals. In this article we introduce a thermodynamic method for estimating the solubilities of model plant surface constituents and relating them to the effects of agrochemicals. Results Following the van Krevelen and Hoftyzer method, we calculated the solubility parameters of three model plant species and eight compounds that differ in hydrophobicity and polarity. In addition, intact tissues were examined by scanning electron microscopy and the surface free energy, polarity, solubility parameter and work of adhesion of each were calculated from contact angle measurements of three liquids with different polarities. By comparing the affinities between plant surface constituents and agrochemicals derived from (a) theoretical calculations and (b) contact angle measurements we were able to distinguish the physical effect of surface roughness from the effect of the chemical nature of the epicuticular waxes. A solubility parameter model for plant surfaces is proposed on the basis of an increasing gradient from the cuticular surface towards the underlying cell wall. Conclusions The procedure enabled us to predict the interactions among agrochemicals, plant surfaces, and cuticular and cell wall components, and promises to be a useful tool for improving our understanding of biological surface interactions. PMID:23151272

Estimation of the solubility parameters of model plant surfaces and agrochemicals: a valuable tool for understanding plant surface interactions.

PubMed

Khayet, Mohamed; Fernández, Victoria

2012-11-14

Most aerial plant parts are covered with a hydrophobic lipid-rich cuticle, which is the interface between the plant organs and the surrounding environment. Plant surfaces may have a high degree of hydrophobicity because of the combined effects of surface chemistry and roughness. The physical and chemical complexity of the plant cuticle limits the development of models that explain its internal structure and interactions with surface-applied agrochemicals. In this article we introduce a thermodynamic method for estimating the solubilities of model plant surface constituents and relating them to the effects of agrochemicals. Following the van Krevelen and Hoftyzer method, we calculated the solubility parameters of three model plant species and eight compounds that differ in hydrophobicity and polarity. In addition, intact tissues were examined by scanning electron microscopy and the surface free energy, polarity, solubility parameter and work of adhesion of each were calculated from contact angle measurements of three liquids with different polarities. By comparing the affinities between plant surface constituents and agrochemicals derived from (a) theoretical calculations and (b) contact angle measurements we were able to distinguish the physical effect of surface roughness from the effect of the chemical nature of the epicuticular waxes. A solubility parameter model for plant surfaces is proposed on the basis of an increasing gradient from the cuticular surface towards the underlying cell wall. The procedure enabled us to predict the interactions among agrochemicals, plant surfaces, and cuticular and cell wall components, and promises to be a useful tool for improving our understanding of biological surface interactions.

[Chemical constituents in aerial part of Reineckea carnea].

PubMed

Xu, Xin; Fu, Hong-Zheng

2008-10-01

To study the chemical constituents in the aerial part of Reineckea carnea. The compounds were isolated by extraction, silica gel, gel, and reversed-phase silica gel coloum chromatography, and high-performance liquid chromatography. The structures were identified by various spectroscopic methods including 1D and 2D NMR spectrum, MS, IR, etc. Six compounds were isolated and identified as 1alpha, 3beta-dihydroxy-5beta-pregn-16-en-20-one-3-O-beta-D-glucopyranoside (1), syringaresinol-beta-D-glucoside (2), sophoraflavone B (3), stigmast-5, 22-dien-3-O-beta-D-glucopyranoside (4), daucosterol (5), a-D-glucose (6). Compound 1 was a new compound, coumpounds 2-6 were obtained from the plant for the first time.

Midgut serine proteases and alternative host plant utilization in Pieris brassicae L.

PubMed Central

Kumar, Rakesh; Bhardwaj, Usha; Kumar, Pawan; Mazumdar-Leighton, Sudeshna

2015-01-01

Pieris brassicae L. is a serious pest of cultivated crucifers in several parts of the world. Larvae of P. brassicae also feed prolifically on garden nasturtium (Tropaeolum majus L., of the family Tropaeolaceae). Proteolytic digestion was studied in larvae feeding on multiple hosts. Fourth instars were collected from cauliflower fields before transfer onto detached, aerial tissues of selected host plants in the lab. Variable levels of midgut proteases were detected in larvae fed on different hosts using protein substrates (casein and recombinant RBCL cloned from cauliflower) and diagnostic, synthetic substrates. Qualitative changes in midgut trypsin activities and quantitative changes in midgut chymotrypsin activities were implicated in physiological adaptation of larvae transferred to T. majus. Midgut proteolytic activities were inhibited to different extents by serine protease inhibitors, including putative trypsin inhibitors isolated from herbivore-attacked and herbivore-free leaves of cauliflower (CfTI) and T. majus (TpTI). Transfer of larvae to T. majus significantly influenced feeding parameters but not necessarily when transferred to different tissues of the same host. Results obtained are relevant for devising sustainable pest management strategies, including transgenic approaches using genes encoding plant protease inhibitors. PMID:25873901

The Plant-Dependent Life Cycle of Thecaphora thlaspeos: A Smut Fungus Adapted to Brassicaceae.

PubMed

Frantzeskakis, Lamprinos; Courville, Kaitlyn J; Plücker, Lesley; Kellner, Ronny; Kruse, Julia; Brachmann, Andreas; Feldbrügge, Michael; Göhre, Vera

2017-04-01

Smut fungi are globally distributed plant pathogens that infect agriculturally important crop plants such as maize or potato. To date, molecular studies on plant responses to smut fungi are challenging due to the genetic complexity of their host plants. Therefore, we set out to investigate the known smut fungus of Brassicaceae hosts, Thecaphora thlaspeos. T. thlaspeos infects different Brassicaceae plant species throughout Europe, including the perennial model plant Arabis alpina. In contrast to characterized smut fungi, mature and dry T. thlaspeos teliospores germinated only in the presence of a plant signal. An infectious filament emerges from the teliospore, which can proliferate as haploid filamentous cultures. Haploid filaments from opposite mating types mate, similar to sporidia of the model smut fungus Ustilago maydis. Consistently, the a and b mating locus genes are conserved. Infectious filaments can penetrate roots and aerial tissues of host plants, causing systemic colonization along the vasculature. Notably, we could show that T. thlaspeos also infects Arabidopsis thaliana. Exploiting the genetic resources of A. thaliana and Arabis alpina will allow us to characterize plant responses to smut infection in a comparative manner and, thereby, characterize factors for endophytic growth as well as smut fungi virulence in dicot plants.

Endemic Sardinian plants: the case of Genista cadasonensis Valsecchi.

PubMed

Serrilli, Anna Maria; Graziosi, Valentina; Ballero, Mauro; Foddis, Caterina; Serafini, Mauro; Poli, Ferruccio; Scartezzini, Paolo; Bianco, Armandodoriano

2010-06-01

The flavonoid fraction of the aerial parts of Genista cadasonensis Valsecchi (Leguminosae), an endemic plant from Sardinia, was examined and compared with the flavonoid pattern already known in the Genista genus. This comparison evidenced the endemic nature of this species, showing the presence of a rare flavonoid, the 6-hydroxy-genistein. The antioxidant activity of dichloromethanic, ethanolic and acetonic total extracts of aerial parts was evaluated.

Online Aerial Terrain Mapping for Ground Robot Navigation

PubMed Central

Peterson, John; Chaudhry, Haseeb; Abdelatty, Karim; Bird, John; Kochersberger, Kevin

2018-01-01

This work presents a collaborative unmanned aerial and ground vehicle system which utilizes the aerial vehicle’s overhead view to inform the ground vehicle’s path planning in real time. The aerial vehicle acquires imagery which is assembled into a orthomosaic and then classified. These terrain classes are used to estimate relative navigation costs for the ground vehicle so energy-efficient paths may be generated and then executed. The two vehicles are registered in a common coordinate frame using a real-time kinematic global positioning system (RTK GPS) and all image processing is performed onboard the unmanned aerial vehicle, which minimizes the data exchanged between the vehicles. This paper describes the architecture of the system and quantifies the registration errors between the vehicles. PMID:29461496

Online Aerial Terrain Mapping for Ground Robot Navigation.

PubMed

Peterson, John; Chaudhry, Haseeb; Abdelatty, Karim; Bird, John; Kochersberger, Kevin

2018-02-20

This work presents a collaborative unmanned aerial and ground vehicle system which utilizes the aerial vehicle's overhead view to inform the ground vehicle's path planning in real time. The aerial vehicle acquires imagery which is assembled into a orthomosaic and then classified. These terrain classes are used to estimate relative navigation costs for the ground vehicle so energy-efficient paths may be generated and then executed. The two vehicles are registered in a common coordinate frame using a real-time kinematic global positioning system (RTK GPS) and all image processing is performed onboard the unmanned aerial vehicle, which minimizes the data exchanged between the vehicles. This paper describes the architecture of the system and quantifies the registration errors between the vehicles.

Impact of understory vegetation on soil carbon and nitrogen dynamic in aerially seeded Pinus massoniana plantations.

PubMed

Pan, Ping; Zhao, Fang; Ning, Jinkui; Zhang, Ling; Ouyang, Xunzhi; Zang, Hao

2018-01-01

Understory vegetation plays a vital role in regulating soil carbon (C) and nitrogen (N) characteristics due to differences in plant functional traits. Different understory vegetation types have been reported following aerial seeding. While aerial seeding is common in areas with serious soil erosion, few studies have been conducted to investigate changes in soil C and N cycling as affected by understory vegetation in aerially seeded plantations. Here, we studied soil C and N characteristics under two naturally formed understory vegetation types (Dicranopteris and graminoid) in aerially seeded Pinus massoniana Lamb plantations. Across the two studied understory vegetation types, soil organic C was significantly correlated with all measured soil N variables, including total N, available N, microbial biomass N and water-soluble organic N, while microbial biomass C was correlated with all measured variables except soil organic C. Dicranopteris and graminoid differed in their effects on soil C and N process. Except water-soluble organic C, all the other C and N variables were higher in soils with graminoids. The higher levels of soil organic C, microbial biomass C, total N, available N, microbial biomass N and water-soluble organic N were consistent with the higher litter and root quality (C/N) of graminoid vegetation compared to Dicranopteris. Changes in soil C and N cycles might be impacted by understory vegetation types via differences in litter or root quality.

Impact of understory vegetation on soil carbon and nitrogen dynamic in aerially seeded Pinus massoniana plantations

PubMed Central

Pan, Ping; Zhao, Fang; Ning, Jinkui; Ouyang, Xunzhi; Zang, Hao

2018-01-01

Understory vegetation plays a vital role in regulating soil carbon (C) and nitrogen (N) characteristics due to differences in plant functional traits. Different understory vegetation types have been reported following aerial seeding. While aerial seeding is common in areas with serious soil erosion, few studies have been conducted to investigate changes in soil C and N cycling as affected by understory vegetation in aerially seeded plantations. Here, we studied soil C and N characteristics under two naturally formed understory vegetation types (Dicranopteris and graminoid) in aerially seeded Pinus massoniana Lamb plantations. Across the two studied understory vegetation types, soil organic C was significantly correlated with all measured soil N variables, including total N, available N, microbial biomass N and water-soluble organic N, while microbial biomass C was correlated with all measured variables except soil organic C. Dicranopteris and graminoid differed in their effects on soil C and N process. Except water-soluble organic C, all the other C and N variables were higher in soils with graminoids. The higher levels of soil organic C, microbial biomass C, total N, available N, microbial biomass N and water-soluble organic N were consistent with the higher litter and root quality (C/N) of graminoid vegetation compared to Dicranopteris. Changes in soil C and N cycles might be impacted by understory vegetation types via differences in litter or root quality. PMID:29377926

Characterization of Cadmium Uptake by Plant Tissue 12

PubMed Central

Cutler, Jay M.; Rains, Donald W.

1974-01-01

The uptake of cadmium by excised root tissue of barley (Hordeum vulgare L. cv. Arivat) was investigated with respect to kinetics, concentration, and interactions with various cations. The role of metabolism in Cd absorption was examined using a range of temperatures, anaerobic treatments, and chemical inhibitors. The uptake and distribution of Cd in intact barley plants was also determined. A large fraction of the Cd taken up by excised barley roots was apparently the result of exchange adsorption and was displaced by subsequent desorption with unlabeled Cd, Zn, Cu, or Hg. Another fraction of Cd which could not be displaced by desorption in unlabeled Cd was thought to result from strong irreversible binding of Cd, perhaps on sites of the cell wall. The fraction of the Cd taken up beyond that by exchange adsorption by fresh roots was a linear function of temperature, and inhibited by conditions of low oxygen and by the presence of 2,4-dinitrophenol. It was concluded that this fraction of Cd entered excised barley roots by diffusion. Diffusion, when followed by sequestering, probably accounts for the accumulation of Cd observed in intact barley plants. PMID:16658840

Reliable aerial thermography for energy conservation

NASA Technical Reports Server (NTRS)

Jack, J. R.; Bowman, R. L.

1981-01-01

A method for energy conservation, the aerial thermography survey, is discussed. It locates sources of energy losses and wasteful energy management practices. An operational map is presented for clear sky conditions. The map outlines the key environmental conditions conductive to obtaining reliable aerial thermography. The map is developed from defined visual and heat loss discrimination criteria which are quantized based on flat roof heat transfer calculations.

CONTEXTUAL AERIAL VIEW OF "EXCLUSION" MTR AREA WITH IDAHO CHEMICAL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

CONTEXTUAL AERIAL VIEW OF "EXCLUSION" MTR AREA WITH IDAHO CHEMICAL PROCESSING PLANT IN BACKGROUND AT CENTER TOP OF VIEW. CAMERA FACING EAST. EXCLUSION GATE HOUSE AT LEFT OF VIEW. BEYOND MTR BUILDING AND ITS WING, THE PROCESS WATER BUILDING AND WORKING RESERVOIR ARE LEFT-MOST. FAN HOUSE AND STACK ARE TO ITS RIGHT. PLUG STORAGE BUILDING IS RIGHT-MOST STRUCTURE. NOTE FAN LOFT ABOVE MTR BUILDING'S ONE-STORY WING. THIS WAS LATER CONVERTED FOR OFFICES. INL NEGATIVE NO. 3610. Unknown Photographer, 10/30/1951 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

Glucosinolate profile and distribution among plant tissues and phenological stages of field-grown horseradish.

PubMed

Agneta, Rosa; Lelario, Filomena; De Maria, Susanna; Möllers, Christian; Bufo, Sabino Aurelio; Rivelli, Anna Rita

2014-10-01

Profile and distribution of glucosinolates (GLS) were detected in plant tissues of horseradish at different developmental stages: beginning of vegetative re-growth, flowering and silique formation. The GLS profile varied widely in the different tissues: we identified 17 GLS in roots and sprouts, one of which was not previously characterized in horseradish, i.e. the 2(S)-hydroxy-2-phenylethyl-GLS (glucobarbarin) and/or 2(R)-hydroxy-2-phenylethyl-GLS (epiglucobarbarin), 11 already found in the roots, including the putative 2-methylsulfonyl-oxo-ethyl-GLS, and 5 previously recognized only in the sprouts. Fifteen of those GLS were also identified in young and cauline leaves, 12 in the mature leaves and 13 in the inflorescences. No difference in GLS profile was observed in plant among the phenological stages. Differences in concentrations of GLS, quantified as desulfated, were found in plant. At the beginning of vegetative re-growth, sprouts while showing the same profile of the roots were much richer in GLS having the highest total GLS concentrations (117.5 and 7.7μmolg(-1) dry weight in sprouts and roots, respectively). During flowering and silique forming stages, the roots still maintained lower amount of total GLS (7.4μmolg(-1) of dry weight, on average) with respect to the epigeous tissues, in which mature and young leaves showed the highest total concentrations (70.5 and 73.8μmolg(-1) of dry weight on average, respectively). Regardless of the phenological stages, the aliphatic GLS were always predominant in all tissues (95%) followed by indolic (2.6%) and benzenic (2.4%) GLS. Sinigrin contributed more than 90% of the total GLS concentration. Aliphatic GLS concentrations were much higher in the epigeous tissues, particularly in the mature and young leaves, while benzenic and indolic GLS concentrations were higher in the roots. Through the phenological stages, GLS concentration increased in young and mature leaves and decreased in cauline leaves and inflorescences

High clearance phenotyping systems for season-long measurement of corn, sorghum and other row crops to complement unmanned aerial vehicle systems

NASA Astrophysics Data System (ADS)

Murray, Seth C.; Knox, Leighton; Hartley, Brandon; Méndez-Dorado, Mario A.; Richardson, Grant; Thomasson, J. Alex; Shi, Yeyin; Rajan, Nithya; Neely, Haly; Bagavathiannan, Muthukumar; Dong, Xuejun; Rooney, William L.

2016-05-01

The next generation of plant breeding progress requires accurately estimating plant growth and development parameters to be made over routine intervals within large field experiments. Hand measurements are laborious and time consuming and the most promising tools under development are sensors carried by ground vehicles or unmanned aerial vehicles, with each specific vehicle having unique limitations. Previously available ground vehicles have primarily been restricted to monitoring shorter crops or early growth in corn and sorghum, since plants taller than a meter could be damaged by a tractor or spray rig passing over them. Here we have designed two and already constructed one of these self-propelled ground vehicles with adjustable heights that can clear mature corn and sorghum without damage (over three meters of clearance), which will work for shorter row crops as well. In addition to regular RGB image capture, sensor suites are incorporated to estimate plant height, vegetation indices, canopy temperature and photosynthetically active solar radiation, all referenced using RTK GPS to individual plots. These ground vehicles will be useful to validate data collected from unmanned aerial vehicles and support hand measurements taken on plots.

A new flavonol glucoside from the aerial parts of Sida glutinosa.

PubMed

Das, Niranjan; Achari, Basudev; Harigaya, Yoshihiro; Dinda, Biswanath

2011-10-01

Phytochemical investigation on the dried aerial parts of Sida glutinosa has led to the isolation of a new flavonol glucoside, glutinoside (1), along with seven known compounds, 24(28)-dehydromakisterone A (2), 1,2,3,9-tetrahydropyrrolo[2,1-b]-quinazolin-3-amine (3), docosanoic acid, 1-triacontanol, campesterol, stigmasterol, and β-sitosterol. The structures of these compounds were elucidated by means of extensive spectroscopic techniques as well as GC/MS analysis (for sterols) and comparison with the literature data. All these seven known compounds are reported from this plant for the first time.

A preliminary evaluation of antihyperglycemic and analgesic activity of Alternanthera sessilis aerial parts

PubMed Central

2014-01-01

Background Alternanthera sessilis is used by folk medicinal practitioners of Bangladesh for alleviation of severe pain. The objective of this study was to scientifically analyze the analgesic (non-narcotic) property of aerial parts of the plant along with antihyperglycemic activity. Methods Antihyperglycemic activity was measured by oral glucose tolerance tests. Analgesic (non-narcotic) activity was determined by observed decreases in abdominal writhings in intraperitoneally administered acetic acid-induced pain model in mice. Results Administration of methanol extract of aerial parts led to dose-dependent and significant reductions in blood glucose levels in glucose-loaded mice. At doses of 50, 100, 200 and 400 mg per kg body weight, the extract reduced blood sugar levels by 22.9, 30.7, 45.4 and 46.1%, respectively compared to control animals. By comparison, a standard antihyperglycemic drug, glibenclamide, when administered at a dose of 10 mg per kg body weight, reduced blood glucose level by 48.9%. In analgesic activity tests, the extract at the above four doses reduced the number of abdominal writhings by 27.6, 37.9, 41.4, and 44.8%, respectively. A standard analgesic drug, aspirin, reduced the number of writhings by 31.0 and 51.7%, respectively, when administered at doses of 200 and 400 mg per kg body weight. Conclusion The results validate the folk medicinal use of the plant to alleviate pain. At the same time, the antihyperglycemic activity result suggests that the plant may be a potential source for blood sugar lowering drug(s). PMID:24885344

USGS Releases New Digital Aerial Products

USGS Publications Warehouse

,

2005-01-01

The U.S. Geological Survey (USGS) Center for Earth Resources Observation and Science (EROS) has initiated distribution of digital aerial photographic products produced by scanning or digitizing film from its historical aerial photography film archive. This archive, located in Sioux Falls, South Dakota, contains thousands of rolls of film that contain more than 8 million frames of historic aerial photographs. The largest portion of this archive consists of original film acquired by Federal agencies from the 1930s through the 1970s to produce 1:24,000-scale USGS topographic quadrangle maps. Most of this photography is reasonably large scale (USGS photography ranges from 1:8,000 to 1:80,000) to support the production of the maps. Two digital products are currently available for ordering: high-resolution scanned products and medium-resolution digitized products.

Cell surface and cell outline imaging in plant tissues using the backscattered electron detector in a variable pressure scanning electron microscope

PubMed Central

2013-01-01

Background Scanning electron microscopy (SEM) has been used for high-resolution imaging of plant cell surfaces for many decades. Most SEM imaging employs the secondary electron detector under high vacuum to provide pseudo-3D images of plant organs and especially of surface structures such as trichomes and stomatal guard cells; these samples generally have to be metal-coated to avoid charging artefacts. Variable pressure-SEM allows examination of uncoated tissues, and provides a flexible range of options for imaging, either with a secondary electron detector or backscattered electron detector. In one application, we used the backscattered electron detector under low vacuum conditions to collect images of uncoated barley leaf tissue followed by simple quantification of cell areas. Results Here, we outline methods for backscattered electron imaging of a variety of plant tissues with particular focus on collecting images for quantification of cell size and shape. We demonstrate the advantages of this technique over other methods to obtain high contrast cell outlines, and define a set of parameters for imaging Arabidopsis thaliana leaf epidermal cells together with a simple image analysis protocol. We also show how to vary parameters such as accelerating voltage and chamber pressure to optimise imaging in a range of other plant tissues. Conclusions Backscattered electron imaging of uncoated plant tissue allows acquisition of images showing details of plant morphology together with images of high contrast cell outlines suitable for semi-automated image analysis. The method is easily adaptable to many types of tissue and suitable for any laboratory with standard SEM preparation equipment and a variable-pressure-SEM or tabletop SEM. PMID:24135233

Aerial and soil seed banks enable populations of an annual species to cope with an unpredictable dune ecosystem

PubMed Central

Gao, Ruiru; Yang, Xuejun; Yang, Fan; Wei, Lingling; Huang, Zhenying; Walck, Jeffrey L.

2014-01-01

Background and Aims Simultaneous formation of aerial and soil seed banks by a species provides a mechanism for population maintenance in unpredictable environments. Eolian activity greatly affects growth and regeneration of plants in a sand dune system, but we know little about the difference in the contributions of these two seed banks to population dynamics in sand dunes. Methods Seed release, germination, seedling emergence and survival of a desert annual, Agriophyllum squarrosum (Chenopodiaceae), inhabiting the Ordos Sandland in China, were determined in order to explore the different functions of the aerial and soil seed banks. Key Results The size of the aerial seed bank was higher than that of the soil seed bank throughout the growing season. Seed release was positively related to wind velocity. Compared with the soil seed bank, seed germination from the aerial seed bank was lower at low temperature (5/15 °C night/day) but higher in the light. Seedling emergence from the soil seed bank was earlier than that from the aerial seed bank. Early-emerged (15 April–15 May) seedlings died due to frost, but seedlings that emerged during the following months survived to reproduce successfully. Conclusions The timing of seed release and different germination behaviour resulted in a temporal heterogeneity of seedling emergence and establishment between the two seed banks. The study suggests that a bet-hedging strategy for the two seed banks enables A. squarrosum populations to cope successfully with the unpredictable desert environment. PMID:24918206

Species and tissue type regulate long-term decomposition of brackish marsh plants grown under elevated CO2 conditions

NASA Astrophysics Data System (ADS)

Jones, Joshua A.; Cherry, Julia A.; McKee, Karen L.

2016-02-01

Organic matter accumulation, the net effect of plant production and decomposition, contributes to vertical soil accretion in coastal wetlands, thereby playing a key role in whether they keep pace with sea-level rise. Any factor that affects decomposition may affect wetland accretion, including atmospheric CO2 concentrations. Higher CO2 can influence decomposition rates by altering plant tissue chemistry or by causing shifts in plant species composition or biomass partitioning. A combined greenhouse-field experiment examined how elevated CO2 affected plant tissue chemistry and subsequent decomposition of above- and belowground tissues of two common brackish marsh species, Schoenoplectus americanus (C3) and Spartina patens (C4). Both species were grown in monoculture and in mixture under ambient (350-385 μL L-1) or elevated (ambient + 300 μL L-1) atmospheric CO2 conditions, with all other growth conditions held constant, for one growing season. Above- and belowground tissues produced under these treatments were decomposed under ambient field conditions in a brackish marsh in the Mississippi River Delta, USA. Elevated CO2 significantly reduced nitrogen content of S. americanus, but not sufficiently to affect subsequent decomposition. Instead, long-term decomposition (percent mass remaining after 280 d) was controlled by species composition and tissue type. Shoots of S. patens had more mass remaining (41 ± 2%) than those of S. americanus (12 ± 2%). Belowground material decomposed more slowly than that placed aboveground (62 ± 1% vs. 23 ± 3% mass remaining), but rates belowground did not differ between species. Increases in atmospheric CO2 concentration will likely have a greater effect on overall decomposition in this brackish marsh community through shifts in species dominance or biomass allocation than through effects on tissue chemistry. Consequent changes in organic matter accumulation may alter marsh capacity to accommodate sea-level rise through vertical

An aerial radiological survey of the Browns Ferry Nuclear Power Station and surrounding area, Decatur, Alabama

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berry, H.A.

1986-10-01

An aerial radiological survey was conducted during the period 12 July to 17 July 1985 over a 97-square-kilometer (37-square-mile) area centered on the Browns Ferry Nuclear Power Station located near Decatur, Alabama. The survey was conducted at a nominal altitude of 46 meters (150 feet) with line spacings of 76 meters (250 feet). A contour map of the terrestrial gamma exposure rate plus the cosmic exposure rate extrapolated to 1 meter above ground level was prepared and overlaid on an aerial photograph and a USGS topographic map of the area. The terrestrial exposure rates north and east of Wheeler Lakemore » ranged from approximately 11 to 18 microroentgens per hour (..mu..R/h), while the area south and west of the lake ranged from approximately 9 to 11 ..mu..R/h. Two areas of increased exposure rate were evident. One of these areas was associated with the main units of the reactor facility, while the other was associated with a temporary radiological waste holding area inside the plant's protected area. A machine-aided search of the data for man-made sources of radiation indicated the presence of a third area within the plant facility. This area is utilized as a temporary low-level radioactive waste storage site. All three areas indicated the presence of Co-60. Soil samples and ion chamber measurements were obtained at four locations to support the aerial data. In addition, soil samples were also taken at six locations along the shore of Wheeler Lake. 8 refs., 13 figs., 4 tabs.« less

Cadmium uptake, accumulation, and remobilization in iron plaque and rice tissues at different growth stages.

PubMed

Zhou, Hang; Zhu, Wei; Yang, Wen-Tao; Gu, Jiao-Feng; Gao, Zi-Xiang; Chen, Li-Wei; Du, Wen-Qi; Zhang, Ping; Peng, Pei-Qin; Liao, Bo-Han

2018-05-15

Rice consumption is considered the main source of human dietary Cd intake in Southeast Asia. This study aimed to investigate Cd uptake, accumulation, and remobilization in iron plaque and rice (Oryza sativa L. cv. 'Xiangwanxian 12') tissues at different growth stages. A pot experiment was performed in two Cd-contaminated paddy soils. Cd concentrations in iron plaque and rice tissues at five different growth stages (tillering, booting, milky, dough, and maturing) were measured. Cd concentrations in iron plaque and rice tissues (roots, stems, leaves, spikelet, husks, and brown rice) varied with growth stage. Cd accumulation in rice plants increased with extending growth in both soils, reaching 15.3 and 35.4μg/pot, respectively, at the maturing stage. The amounts of Cd in brown rice increased from the milky to maturing stages, with the greatest percentage uptake during the maturing stage. Cd amount in iron plaque significantly affected the uptake and accumulation of Cd in roots and aerial parts of rice plants. Accumulated Cd in leaves was remobilized and transported during the booting to maturing stages, and the contributions of Cd transportation from leaves to brown rice were 30.0% and 22.5% in the two soils, respectively. A large amount of Cd accumulated in brown rice during the maturing stage. The transportation of remobilized Cd from leaves was also important for the accumulation of Cd in brown rice. Copyright © 2018 Elsevier Inc. All rights reserved.

Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues

PubMed Central

Ndunguru, Joseph; Taylor, Nigel J; Yadav, Jitender; Aly, Haytham; Legg, James P; Aveling, Terry; Thompson, Graham; Fauquet, Claude M

2005-01-01

Background Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting plants is required to facilitate their molecular study and is essential for the development of crop protection and improvement programs. Retaining integrity of viral pathogens within sampled plant tissues is often a limiting factor in this process, most especially when sample sizes are large and when operating in developing counties and regions remote from laboratory facilities. FTA is a paper-based system designed to fix and store nucleic acids directly from fresh tissues pressed into the treated paper. We report here the use of FTA as an effective technology for sampling and retrieval of DNA and RNA viruses from plant tissues and their subsequent molecular analysis. Results DNA and RNA viruses were successfully recovered from leaf tissues of maize, cassava, tomato and tobacco pressed into FTA® Classic Cards. Viral nucleic acids eluted from FTA cards were found to be suitable for diagnostic molecular analysis by PCR-based techniques and restriction analysis, and for cloning and nucleotide sequencing in a manner equivalent to that offered by tradition isolation methods. Efficacy of the technology was demonstrated both from sampled greenhouse-grown plants and from leaf presses taken from crop plants growing in farmer's fields in East Africa. In addition, FTA technology was shown to be suitable for recovery of viral-derived transgene sequences integrated into the plant genome. Conclusion Results demonstrate that FTA is a practical, economical and sensitive method for sampling, storage and retrieval of viral pathogens and plant genomic sequences, when working under controlled conditions and in the field. Application of this technology has the potential to significantly increase ability to bring modern analytical techniques to bear on the viral pathogens infecting crop plants. PMID:15904535

Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues.

PubMed

Ndunguru, Joseph; Taylor, Nigel J; Yadav, Jitender; Aly, Haytham; Legg, James P; Aveling, Terry; Thompson, Graham; Fauquet, Claude M

2005-05-18

Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting plants is required to facilitate their molecular study and is essential for the development of crop protection and improvement programs. Retaining integrity of viral pathogens within sampled plant tissues is often a limiting factor in this process, most especially when sample sizes are large and when operating in developing counties and regions remote from laboratory facilities. FTA is a paper-based system designed to fix and store nucleic acids directly from fresh tissues pressed into the treated paper. We report here the use of FTA as an effective technology for sampling and retrieval of DNA and RNA viruses from plant tissues and their subsequent molecular analysis. DNA and RNA viruses were successfully recovered from leaf tissues of maize, cassava, tomato and tobacco pressed into FTA Classic Cards. Viral nucleic acids eluted from FTA cards were found to be suitable for diagnostic molecular analysis by PCR-based techniques and restriction analysis, and for cloning and nucleotide sequencing in a manner equivalent to that offered by tradition isolation methods. Efficacy of the technology was demonstrated both from sampled greenhouse-grown plants and from leaf presses taken from crop plants growing in farmer's fields in East Africa. In addition, FTA technology was shown to be suitable for recovery of viral-derived transgene sequences integrated into the plant genome. Results demonstrate that FTA is a practical, economical and sensitive method for sampling, storage and retrieval of viral pathogens and plant genomic sequences, when working under controlled conditions and in the field. Application of this technology has the potential to significantly increase ability to bring modern analytical techniques to bear on the viral pathogens infecting crop plants.

Detection of Laurel Wilt Disease in Avocado Using Low Altitude Aerial Imaging

PubMed Central

de Castro, Ana I.; Ehsani, Reza; Ploetz, Randy C.; Crane, Jonathan H.; Buchanon, Sherrie

2015-01-01

Laurel wilt is a lethal disease of plants in the Lauraceae plant family, including avocado (Persea americana). This devastating disease has spread rapidly along the southeastern seaboard of the United States and has begun to affect commercial avocado production in Florida. The main objective of this study was to evaluate the potential to discriminate laurel wilt-affected avocado trees using aerial images taken with a modified camera during helicopter surveys at low-altitude in the commercial avocado production area. The ability to distinguish laurel wilt-affected trees from other factors that produce similar external symptoms was also studied. RmodGB digital values of healthy trees and laurel wilt-affected trees, as well as fruit stress and vines covering trees were used to calculate several vegetation indices (VIs), band ratios, and VI combinations. These indices were subjected to analysis of variance (ANOVA) and an M-statistic was performed in order to quantify the separability of those classes. Significant differences in spectral values among laurel wilt affected and healthy trees were observed in all vegetation indices calculated, although the best results were achieved with Excess Red (ExR), (Red–Green) and Combination 1 (COMB1) in all locations. B/G showed a very good potential for separate the other factors with symptoms similar to laurel wilt-affected trees, such as fruit stress and vines covering trees, from laurel wilt-affected trees. These consistent results prove the usefulness of using a modified camera (RmodGB) to discriminate laurel wilt-affected avocado trees from healthy trees, as well as from other factors that cause the same symptoms and suggest performing the classification in further research. According to our results, ExR and B/G should be utilized to develop an algorithm or decision rules to classify aerial images, since they showed the highest capacity to discriminate laurel wilt-affected trees. This methodology may allow the rapid

Detection of laurel wilt disease in avocado using low altitude aerial imaging.

PubMed

de Castro, Ana I; Ehsani, Reza; Ploetz, Randy C; Crane, Jonathan H; Buchanon, Sherrie

2015-01-01

Laurel wilt is a lethal disease of plants in the Lauraceae plant family, including avocado (Persea americana). This devastating disease has spread rapidly along the southeastern seaboard of the United States and has begun to affect commercial avocado production in Florida. The main objective of this study was to evaluate the potential to discriminate laurel wilt-affected avocado trees using aerial images taken with a modified camera during helicopter surveys at low-altitude in the commercial avocado production area. The ability to distinguish laurel wilt-affected trees from other factors that produce similar external symptoms was also studied. RmodGB digital values of healthy trees and laurel wilt-affected trees, as well as fruit stress and vines covering trees were used to calculate several vegetation indices (VIs), band ratios, and VI combinations. These indices were subjected to analysis of variance (ANOVA) and an M-statistic was performed in order to quantify the separability of those classes. Significant differences in spectral values among laurel wilt affected and healthy trees were observed in all vegetation indices calculated, although the best results were achieved with Excess Red (ExR), (Red-Green) and Combination 1 (COMB1) in all locations. B/G showed a very good potential for separate the other factors with symptoms similar to laurel wilt-affected trees, such as fruit stress and vines covering trees, from laurel wilt-affected trees. These consistent results prove the usefulness of using a modified camera (RmodGB) to discriminate laurel wilt-affected avocado trees from healthy trees, as well as from other factors that cause the same symptoms and suggest performing the classification in further research. According to our results, ExR and B/G should be utilized to develop an algorithm or decision rules to classify aerial images, since they showed the highest capacity to discriminate laurel wilt-affected trees. This methodology may allow the rapid detection

BOREAS Level-0 ER-2 Aerial Photography

NASA Technical Reports Server (NTRS)

Newcomer, Jeffrey A.; Dominquez, Roseanne; Hall, Forrest G. (Editor)

2000-01-01

For BOReal Ecosystem-Atmosphere Study (BOREAS), the ER-2 and other aerial photography was collected to provide finely detailed and spatially extensive documentation of the condition of the primary study sites. The ER-2 aerial photography consists of color-IR transparencies collected during flights in 1994 and 1996 over the study areas.

7 CFR 1755.703 - Nonmetallic reinforced (NMR) aerial service wire.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 11 2014-01-01 2014-01-01 false Nonmetallic reinforced (NMR) aerial service wire... MATERIALS, AND STANDARD CONTRACT FORMS § 1755.703 Nonmetallic reinforced (NMR) aerial service wire. (a... resistance of each conductor in a completed NMR aerial service wire shall comply with the requirement...

7 CFR 1755.703 - Nonmetallic reinforced (NMR) aerial service wire.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 11 2013-01-01 2013-01-01 false Nonmetallic reinforced (NMR) aerial service wire... MATERIALS, AND STANDARD CONTRACT FORMS § 1755.703 Nonmetallic reinforced (NMR) aerial service wire. (a... resistance of each conductor in a completed NMR aerial service wire shall comply with the requirement...

7 CFR 1755.703 - Nonmetallic reinforced (NMR) aerial service wire.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 11 2012-01-01 2012-01-01 false Nonmetallic reinforced (NMR) aerial service wire... MATERIALS, AND STANDARD CONTRACT FORMS § 1755.703 Nonmetallic reinforced (NMR) aerial service wire. (a... resistance of each conductor in a completed NMR aerial service wire shall comply with the requirement...

Evolutionary and tissue-specific control of expression of multiple acyl-carrier protein isoforms in plants and bacteria.

PubMed

Battey, J F; Ohlrogge, J B

1990-02-01

We have examined the occurrence of multiple acyl-carrier protein (ACP), isoforms in evolutionarily diverse species of higher and lower plants. Isoforms were resolved by native polyacrylamide gel electrophoresis (PAGE), and were detected by Western blotting or fluorography of [(3)H]-palmitate-labelled ACPs. Multiple isoforms of ACP were found in leaf tissue of the monocotyledons Avena sativa and Hordeum vulgare and dicotyledons Arabidopsis thaliana, Cuphea wrightii, and Brassica napus. Lower vascular plants including the lycopod Selaginella krausseriana, the gymnosperms Ephedra sp. and Dioon edule, the ferns Davallia feejensis and Marsilea sp. and the most primitive known extant vascular plant, Psilotum nudum, were all found to have multiple ACP isoforms, as were the nonvascular liverworts, Lunularia sp. and Marchantia sp. and the moss, Polytrichum sp. Therefore, the development of ACP isoforms appears to have occurred early in plant evolution. However, we could detect only a single electrophoretic form of ACP in the unicellular algae Chlamydomonas reinhardtii and Dunaliella tertiolecta and the photosynthetic cyanobacteria Synechocystis strain 6803 and Agmnellum quadruplicatum. Thus, multiple forms of ACP do not occur in all photosynthetic organisms but may be associated with multicellular plants. We have also examined tissue specificity and light control over the expression of ACP isoforms. The relative abundance of multiple forms of ACP in leaf of Spinacia and Avena was altered very little by light. Rather, the different patterns of ACP isoforms were primarily dependent on the tissue type.

[Small unmanned aerial vehicles for low-altitude remote sensing and its application progress in ecology.

PubMed

Sun, Zhong Yu; Chen, Yan Qiao; Yang, Long; Tang, Guang Liang; Yuan, Shao Xiong; Lin, Zhi Wen

2017-02-01

Low-altitude unmanned aerial vehicles (UAV) remote sensing system overcomes the deficiencies of space and aerial remote sensing system in resolution, revisit period, cloud cover and cost, which provides a novel method for ecological research on mesoscale. This study introduced the composition of UAV remote sensing system, reviewed its applications in species, population, community and ecosystem ecology research. Challenges and opportunities of UAV ecology were identified to direct future research. The promising research area of UAV ecology includes the establishment of species morphology and spectral characteristic data base, species automatic identification, the revelation of relationship between spectral index and plant physiological processes, three-dimension monitoring of ecosystem, and the integration of remote sensing data from multi resources and multi scales. With the development of UAV platform, data transformation and sensors, UAV remote sensing technology will have wide application in ecology research.

Off-the-Wall Project Brings Aerial Mapping down to Earth

ERIC Educational Resources Information Center

Davidhazy, Andrew

2008-01-01

The technology of aerial photography, photogrametry, has widespread applications in mapping and aerial surveying. A multi-billion-dollar industry, aerial surveying and mapping is "big business" in both civilian and military sectors. While the industry has grown increasingly automated, employment opportunities still exist for people with a basic…

Knowledge-based understanding of aerial surveillance video

NASA Astrophysics Data System (ADS)

Cheng, Hui; Butler, Darren

2006-05-01

Aerial surveillance has long been used by the military to locate, monitor and track the enemy. Recently, its scope has expanded to include law enforcement activities, disaster management and commercial applications. With the ever-growing amount of aerial surveillance video acquired daily, there is an urgent need for extracting actionable intelligence in a timely manner. Furthermore, to support high-level video understanding, this analysis needs to go beyond current approaches and consider the relationships, motivations and intentions of the objects in the scene. In this paper we propose a system for interpreting aerial surveillance videos that automatically generates a succinct but meaningful description of the observed regions, objects and events. For a given video, the semantics of important regions and objects, and the relationships between them, are summarised into a semantic concept graph. From this, a textual description is derived that provides new search and indexing options for aerial video and enables the fusion of aerial video with other information modalities, such as human intelligence, reports and signal intelligence. Using a Mixture-of-Experts video segmentation algorithm an aerial video is first decomposed into regions and objects with predefined semantic meanings. The objects are then tracked and coerced into a semantic concept graph and the graph is summarized spatially, temporally and semantically using ontology guided sub-graph matching and re-writing. The system exploits domain specific knowledge and uses a reasoning engine to verify and correct the classes, identities and semantic relationships between the objects. This approach is advantageous because misclassifications lead to knowledge contradictions and hence they can be easily detected and intelligently corrected. In addition, the graph representation highlights events and anomalies that a low-level analysis would overlook.

Estimating occupancy and abundance using aerial images with imperfect detection

USGS Publications Warehouse

Williams, Perry J.; Hooten, Mevin B.; Womble, Jamie N.; Bower, Michael R.

2017-01-01

Species distribution and abundance are critical population characteristics for efficient management, conservation, and ecological insight. Point process models are a powerful tool for modelling distribution and abundance, and can incorporate many data types, including count data, presence-absence data, and presence-only data. Aerial photographic images are a natural tool for collecting data to fit point process models, but aerial images do not always capture all animals that are present at a site. Methods for estimating detection probability for aerial surveys usually include collecting auxiliary data to estimate the proportion of time animals are available to be detected.We developed an approach for fitting point process models using an N-mixture model framework to estimate detection probability for aerial occupancy and abundance surveys. Our method uses multiple aerial images taken of animals at the same spatial location to provide temporal replication of sample sites. The intersection of the images provide multiple counts of individuals at different times. We examined this approach using both simulated and real data of sea otters (Enhydra lutris kenyoni) in Glacier Bay National Park, southeastern Alaska.Using our proposed methods, we estimated detection probability of sea otters to be 0.76, the same as visual aerial surveys that have been used in the past. Further, simulations demonstrated that our approach is a promising tool for estimating occupancy, abundance, and detection probability from aerial photographic surveys.Our methods can be readily extended to data collected using unmanned aerial vehicles, as technology and regulations permit. The generality of our methods for other aerial surveys depends on how well surveys can be designed to meet the assumptions of N-mixture models.

Distribution patterns of MCA-coated granules aerially applied to corn fields of Southern Hungary between 2000 and 2002.

PubMed

Wennemann, L; Hummel, H E

2003-01-01

Field studies in corn (Zea mays L.) were conducted to evaluate distribution patterns of 4-methoxy-cinnamaldehyde (MCA) coated corn grits after aerial application with a Dromader fixed wing aircraft. The kairomone mimic MCA is synthetically available and a quite specific and efficient adult attractant for the invasive alien maize pest western corn rootworm (WCR) Diabrotica virgifera virgifera LeConte. Orientation disruptive properties of MCA for WCR when applied at unphysiologically high concentrations are currently under investigation. For successful implementation of the MCA disruption technique, the distribution patterns of MCA coated corn granules ('grits') in the field are important. Grits are degrained corn cobs, shredded to different sizes, coated with MCA and used as a carrier material to disseminate MCA vapors into corn fields. Granules of 10-12 mesh size were aerially applied eight times at rates ranging from 12.4 to 25.0 kg/ha. The goal is to evaluate distribution patterns of corn grits treated with MCA in three fields located at Csanadpalota, Kardoskút and Mezöhegyes in Southern Hungary between 2000 and 2002. Increasing rates reflect our attempts in finding and optimising the most even distribution of granules in the field. Field experiments were evaluated by collecting grits in 30-cm plastic saucers and by counting grits accumulated on corn plant parts. Variation in grit number per unit area and frequency of corn granule number per plant showed some transient technical application problems. Analysis of grits collected in the saucers revealed some statistical difference between the different application dates as well as differences in rates applied. Altogether grits in saucers were more evenly distributed in comparison to the grits collected on plant parts. As the corn plants age, their leaves and whorls present a smaller and smaller surface area where granules can accumulate. Altogether, however, grit distribution patterns indicate that aerial

7 CFR 1755.703 - Nonmetallic reinforced (NMR) aerial service wire.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 11 2011-01-01 2011-01-01 false Nonmetallic reinforced (NMR) aerial service wire... MATERIALS, AND STANDARD CONTRACT FORMS § 1755.703 Nonmetallic reinforced (NMR) aerial service wire. (a..., paragraphs 2.2 and 2.2.1. The ANSI/ICEA S-89-648-1993 Standard For Telecommunications Aerial Service Wire...

7 CFR 1755.703 - Nonmetallic reinforced (NMR) aerial service wire.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false Nonmetallic reinforced (NMR) aerial service wire... MATERIALS, AND STANDARD CONTRACT FORMS § 1755.703 Nonmetallic reinforced (NMR) aerial service wire. (a..., paragraphs 2.2 and 2.2.1. The ANSI/ICEA S-89-648-1993 Standard For Telecommunications Aerial Service Wire...

7 CFR 1755.700 - RUS specification for aerial service wires.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false RUS specification for aerial service wires. 1755.700..., AND STANDARD CONTRACT FORMS § 1755.700 RUS specification for aerial service wires. §§ 1755.701 through 1755.704 cover the requirements for aerial service wires. [61 FR 26074, May 24, 1996] ...

7 CFR 1755.700 - RUS specification for aerial service wires.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 11 2011-01-01 2011-01-01 false RUS specification for aerial service wires. 1755.700..., AND STANDARD CONTRACT FORMS § 1755.700 RUS specification for aerial service wires. §§ 1755.701 through 1755.704 cover the requirements for aerial service wires. [61 FR 26074, May 24, 1996] ...

RNA Isolation from Plant Tissues: A Hands-On Laboratory Experimental Experience for Undergraduates

ERIC Educational Resources Information Center

Zhang, Nianhui; Yu, Dong; Zhu, Xiaofeng

2018-01-01

The practice of RNA isolation in undergraduate experimental courses is rare because of the existence of robust, ubiquitous and stable ribonucleases. We reported here modifications to our original protocol for RNA isolation from plant tissues, including the recovery of nucleic acids by ethanol precipitation at 0 degrees C for 10 min and the…

Fluorescence in situ hybridization for phytoplasma and endophytic bacteria localization in plant tissues.

PubMed

Bulgari, Daniela; Casati, Paola; Faoro, Franco

2011-11-01

In the present study, we developed a rapid and efficient fluorescence in situ hybridization assay (FISH) in non-embedded tissues of the model plant Catharanthus roseus for co-localizing phytoplasmas and endophytic bacteria, opening new perspectives for studying the interaction between these microorganisms. Copyright © 2011 Elsevier B.V. All rights reserved.

Cocowood Fibrovascular Tissue System—Another Wonder of Plant Evolution

PubMed Central

González, Oswaldo M.; Nguyen, Khoi A.

2016-01-01

The coconut palm (Cocos nucifera L.) stem tissue (referred to as cocowood in this study) is a complex fibrovascular system that is made up of fibrovascular bundles embedded into a parenchymatous ground tissue. The complex configuration of fibrovascular bundles along with the non-uniform distribution of the material properties likely allow senile coconut stems to optimize their biomechanical performance per unit mass (i.e., mechanical efficiency) and grow into tall, slender, and very flexible plants with minimum resources of biomass and water. For the first time, to the best of the authors' knowledge, this paper examines, from the integral (i.e., stem structure) and macroscopic (i.e., tissue structure) levels of hierarchy, the characteristic triple helix formation depicted by the fibrovascular bundles within the monocotyledon cocowood. The natural course of the tangential orientation of the axial fibrovascular bundles is mapped for the whole cocowood structure by quantifying 264 cocowood discs, corresponding to 41 senile coconut palms estimated to be >70 years old. The observed variations were modeled in this paper by simple equations that partially enabled characterization of the cocowood fibrovascular tissue system. Furthermore, 11 finite element analyses (FEA) were performed over a three dimensional (3D) finite element (FE) model resembling a characteristic coconut palm stem of 25 m in height to analyze the biomaterial reactions produced by the progressive deviation of the tangential fibrovascular bundles on the cocowood mechanical response (i.e., on the material compressive strength and the bending stiffness). The analyses in this study were carried out for the critical wind speed of 23 m/s (i.e., Gale tornado according to the Fujita tornado scale). For each analysis, the characteristic average maxima degree of orientation of the cocowood fibrovascular bundles was varied from 0° to 51°. The acquired results provided a deep understanding of the cocowood optimum

Effects of chemically characterized fractions from aerial parts of Echinacea purpurea and E. angustifolia on myelopoiesis in rats.

PubMed

Ramasahayam, Sindhura; Baraka, Hany N; Abdel Bar, Fatma M; Abuasal, Bilal S; Widrlechner, Mark P; Sayed, Khalid A El; Meyer, Sharon A

2011-11-01

Echinacea species are used for beneficial effects on immune function, and various prevalent phytochemicals have immunomodulatory effects. Using a commercial E. purpurea (L.) Moench product, we have evaluated the myelopoietic effect on bone marrow of rats treated with various extracts and correlated this with their chemical class composition. Granulocyte/macrophage-colony forming cells (GM-CFCs) from femurs of female Sprague-Dawley rats were assessed at 24 h after 7 daily oral treatments. A 75% ethanolic extract at 50 mg dried weight (derived from 227 mg aerial parts) per kg body weight increased GM-CFCs by 70% but at 100 mg/kg was without effect. Ethanolic extracts from aerial parts of E. angustifolia DC. var. angustifolia and E. purpurea from the USDA North Central Regional Plant Introduction Station increased GM-CFCs by 3- and 2-fold, respectively, at 200 mg/kg (~1400 mg/kg plant material). Extract from another USDA E. angustifolia was inactive. Proton and APT NMR, MS, and TLC indicated alkylamides and caffeic-acid derivatives (CADs) present in ethanolic extracts of both the commercial and USDA-derived material. Cichoric and caftaric acids were prominent in both E. purpurea ethanolic extracts but absent in E. angustifolia. Aqueous extract of the commercial material exhibited polysaccharide and CAD signatures and was without effect on GM-CFCs. A methanol-CHCl3 fraction of commercial source, also inactive, was almost exclusively 1:4 nonanoic: decanoic acids, which were also abundant in commercial ethanolic extract but absent from USDA material. In conclusion, we have demonstrated an ethanolextractable myelostimulatory activity in Echinacea aerial parts that, when obtained from commercial herbal supplements, may be antagonized by medium-chain fatty acids presumably derived from a non-plant additive. © Georg Thieme Verlag KG Stuttgart · New York.

Geography via Aerial Field Trips: Do It This Way, 6.

ERIC Educational Resources Information Center

Richason, Benjamin F., Jr.; Guell, Carl E.

To provide guidance for geography teachers, this booklet presents information on how to plan and execute aerial field trips. The aerial field trip can be employed as an effective visual aid technique in the teaching of geography, especially for presenting earth generalizations and interrelationships. The benefits of an aerial field trip are…

Exogenous Methyl Jasmonate Treatment Increases Glucosinolate Biosynthesis and Quinone Reductase Activity in Kale Leaf Tissue

PubMed Central

Ku, Kang-Mo; Jeffery, Elizabeth H.; Juvik, John A.

2014-01-01

Methyl jasmonate (MeJA) spray treatments were applied to the kale varieties ‘Dwarf Blue Curled Vates’ and ‘Red Winter’ in replicated field plantings in 2010 and 2011 to investigate alteration of glucosinolate (GS) composition in harvested leaf tissue. Aqueous solutions of 250 µM MeJA were sprayed to saturation on aerial plant tissues four days prior to harvest at commercial maturity. The MeJA treatment significantly increased gluconasturtiin (56%), glucobrassicin (98%), and neoglucobrassicin (150%) concentrations in the apical leaf tissue of these genotypes over two seasons. Induction of quinone reductase (QR) activity, a biomarker for anti-carcinogenesis, was significantly increased by the extracts from the leaf tissue of these two cultivars. Extracts of apical leaf tissues had greater MeJA mediated increases in phenolics, glucosinolate concentrations, GS hydrolysis products, and QR activity than extracts from basal leaf tissue samples. The concentration of the hydrolysis product of glucoraphanin, sulforphane was significantly increased in apical leaf tissue of the cultivar ‘Red Winter’ in both 2010 and 2011. There was interaction between exogenous MeJA treatment and environmental conditions to induce endogenous JA. Correlation analysis revealed that indole-3-carbanol (I3C) generated from the hydrolysis of glucobrassicin significantly correlated with QR activity (r = 0.800, P<0.001). Concentrations required to double the specific QR activity (CD values) of I3C was calculated at 230 µM, which is considerably weaker at induction than other isothiocyanates like sulforphane. To confirm relationships between GS hydrolysis products and QR activity, a range of concentrations of MeJA sprays were applied to kale leaf tissues of both cultivars in 2011. Correlation analysis of these results indicated that sulforaphane, NI3C, neoascorbigen, I3C, and diindolylmethane were all significantly correlated with QR activity. Thus, increased QR activity may be due to

Micro-scale elemental partition in tissues of the aquatic plant Lemna minor L. exposed to highway drainage water

NASA Astrophysics Data System (ADS)

Mendes Godinho, R.; Raimundo, J.; Vale, C.; Anes, B.; Brito, P.; Alves, L. C.; Pinheiro, T.

2013-07-01

In the scope of a monitoring program to assess the environmental impact of automobile traffic over one main bridge in Lisbon, both water and duckweed (Lemna minor L.) were sampled from the road drainage tanks and analyzed for chemical elements. Plants uptake Cr, Mn, Cu, and Zn metals from rain water draining the bridge road. Nuclear microprobe elemental maps of cryosections of L. minor tissues showed that incorporated elements were internalized in fronds of the plant. This approach at micrometer level allows a better knowledge of the elemental tissue partitioning in this biomonitor organism.

Spatial organization and correlation properties quantify structural changes on mesoscale of parenchymatous plant tissue

NASA Astrophysics Data System (ADS)

Valous, N. A.; Delgado, A.; Drakakis, K.; Sun, D.-W.

2014-02-01

The study of plant tissue parenchyma's intercellular air spaces contributes to the understanding of anatomy and physiology. This is challenging due to difficulty in making direct measurements of the pore space and the complex mosaic of parenchymatous tissue. The architectural complexity of pore space has shown that single geometrical measurements are not sufficient for characterization. The inhomogeneity of distribution depends not only on the percentage content of phase, but also on how the phase fills the space. The lacunarity morphometric, as multiscale measure, provides information about the distribution of gaps that correspond to degree of spatial organization in parenchyma. Additionally, modern theories have suggested strategies, where the focus has shifted from the study of averages and histograms to the study of patterns in data fluctuations. Detrended fluctuation analysis provides information on the correlation properties of the parenchyma at different spatial scales. The aim is to quantify (with the aid of the aforementioned metrics), the mesostructural changes—that occur from one cycle of freezing and thawing—in the void phase of pome fruit parenchymatous tissue, acquired with X-ray microcomputed tomography. Complex systems methods provide numerical indices and detailed insights regarding the freezing-induced modifications upon the arrangement of cells and voids. These structural changes have the potential to lead to physiological disorders. The work can further stimulate interest for the analysis of internal plant tissue structures coupled with other physico-chemical processes or phenomena.

23. AERIAL VIEW LOOKING SOUTHEAST AT THE PLUTONIUM OPERATION BUILDINGS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. AERIAL VIEW LOOKING SOUTHEAST AT THE PLUTONIUM OPERATION BUILDINGS 771, 776/777, AND 707. BUILDING 771, IN THE FOREGROUND, WAS BUILT IN 1952 TO HOUSE ALL PLUTONIUM OPERATIONS. BY 1956, BUILDING 771 WAS NO LONGER ADEQUATE FOR PRODUCTION DEMANDS. BUILDING 776/777, TO THE SOUTH OF BUILDING 771, WAS CONSTRUCTED TO HOUSE PLUTONIUM FABRICATION AND FOUNDRY OPERATIONS. PLUTONIUM RECOVERY REMAINED IN BUILDING 771. BY 1967, CONSTRUCTION ON BUILDING 707, TO THE SOUTH OF BUILDING 776/777, BEGAN AS PRODUCTION LEVELS CONTINUED TO EXPAND NECESSITATING THE NEED FOR ADDITIONAL PLUTONIUM FABRICATION SPACE (7/1/69). - Rocky Flats Plant, Bounded by Indiana Street & Routes 93, 128 & 72, Golden, Jefferson County, CO

Combining LANDSAT MSS, aerial photographs and ground measurements to estimate rangeland productivity

NASA Technical Reports Server (NTRS)

Gialdini, M. J.

1981-01-01

The production of a vegetation map of over 2.2 million acres with detail down to the plant community level, and the production of estimates of rangeland productivity (pounds of usable forage per acre for cattle) for a 500,000 acre subset of area with a design goal for accuracy and precision of + or - 20% at the 80% confidence level, are considered. The data consist of five groups: maps of area, LANDSAT data, digital terrain data, large scale aerial photography, and ground plots. An outline of the data acquisition and data reduction schemes are presented.

Tissue Culture as a Source of Replicates in Nonmodel Plants: Variation in Cold Response in Arabidopsis lyrata ssp. petraea.

PubMed

Kenta, Tanaka; Edwards, Jessica E M; Butlin, Roger K; Burke, Terry; Quick, W Paul; Urwin, Peter; Davey, Matthew P

2016-12-07

While genotype-environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates-a challenging hurdle in outcrossing plants. This could be potentially overcome by using tissue culture techniques. However, plants regenerated from tissue culture may show aberrant phenotypes and "somaclonal" variation. Here, we examined somaclonal variation due to tissue culturing using the response to cold treatment of photosynthetic efficiency (chlorophyll fluorescence measurements for F v /F m , F v '/F m ', and Φ PSII , representing maximum efficiency of photosynthesis for dark- and light-adapted leaves, and the actual electron transport operating efficiency, respectively, which are reliable indicators of photoinhibition and damage to the photosynthetic electron transport system). We compared this to variation among half-sibling seedlings from three different families of Arabidopsis lyrata ssp. petraea Somaclonal variation was limited, and we could detect within-family variation in change in chlorophyll fluorescence due to cold shock successfully with the help of tissue-culture derived replicates. Icelandic and Norwegian families exhibited higher chlorophyll fluorescence, suggesting higher performance after cold shock, than a Swedish family. Although the main effect of tissue culture on F v /F m , F v '/F m ', and Φ PSII was small, there were significant interactions between tissue culture and family, suggesting that the effect of tissue culture is genotype-specific. Tissue-cultured plantlets were less affected by cold treatment than seedlings, but to a different extent in each family. These interactive effects, however, were comparable to, or much smaller than the single effect of family. These results suggest that tissue culture is a useful method for obtaining genetically homogenous replicates for studying genotype-environment interaction related to adaptively-relevant phenotypes, such

Diurnal Changes in Volume and Specific Tissue Weight of Crassulacean Acid Metabolism Plants 1

PubMed Central

Chen, Sheng-Shu; Black, Clanton C.

1983-01-01

The diurnal variations in volume and in specific weight were determined for green stems and leaves of Crassulacen acid metabolism (CAM) plants. Volume changes were measured by a water displacement method. Diurnal variations occurred in the volume of green CAM tissues. Their volume increased early in the light period reaching a maximum about mid-day, then the volume decreased to a minimum near midnight. The maximum volume increase each day was about 2.7% of the total volume. Control leaves of C3 and C4 plants exhibited reverse diurnal volume changes of 0.2 to 0.4%. The hypothesis is presented and supported that green CAM tissues should exhibit a diurnal increase in volume due to the increase of internal gas pressure from CO2 and O2 when their stomata are closed. Conversely, the volume should decrease when the gas pressure is decreased. The second hypothesis presented and supported was that the specific weight (milligrams of dry weight per square centimeter of green surface area) of green CAM tissues should increase at night due to the net fixation of CO2. Green CAM tissues increased their specific weight at night in contrast to control C3 and C4 leaves which decreased their specific weight at night. With Kalanchoë daigremontiana leaves, the calculated increase in specific leaf weight at night based on estimates of carbohydrate available for net CO2 fixation was near 6% and the measured increase in specific leaf weight was 6%. Diurnal measurements of CAM tissue water content were neither coincident nor reciprocal with their diurnal patterns of either volume or specific weight changes. PMID:16662833

Effects of pesticides aerial applications on rice quality

USDA-ARS?s Scientific Manuscript database

Aerial application of pesticides has become an important research topic in recent years. This research investigated the effects of two types of commercial pesticides on the rice quality under low volume aerial application. It could provide guidance for the pesticide application and choose the right ...

International-Aerial Measuring System (I-AMS) Training Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wasiolek, Piotre T.; Malchor, Russell L.; Maurer, Richard J.

2015-10-01

Since the Fukushima reactor accident in 2011, there has been an increased interest worldwide in developing national capabilities to rapidly map and assess ground contamination resulting from nuclear reactor accidents. The capability to rapidly measure the size of the contaminated area, determine the activity level, and identify the radionuclides can aid emergency managers and decision makers in providing timely protective action recommendations to the public and first responders. The development of an aerial detection capability requires interagency coordination to assemble the radiation experts, detection system operators, and aviation aircrews to conduct the aerial measurements, analyze and interpret the data, andmore » provide technical assessments. The Office of International Emergency Management and Cooperation (IEMC) at the U.S. Department of Energy, National Nuclear Security Administration (DOE/NNSA) sponsors an International - Aerial Measuring System (I-AMS) training program for partner nations to develop and enhance their response to radiological emergencies. An initial series of courses can be conducted in the host country to assist in developing an aerial detection capability. As the capability develops and expands, additional experience can be gained through advanced courses with the opportunity to conduct aerial missions over a broad range of radiation environments.« less

Initial Efforts toward Mission-Representative Imaging Surveys from Aerial Explorers

NASA Technical Reports Server (NTRS)

Pisanich, Greg; Plice, Laura; Ippolito, Corey; Young, Larry A.; Lau, Benton; Lee, Pascal

2004-01-01

Numerous researchers have proposed the use of robotic aerial explorers to perform scientific investigation of planetary bodies in our solar system. One of the essential tasks for any aerial explorer is to be able to perform scientifically valuable imaging surveys. The focus of this paper is to discuss the challenges implicit in, and recent observations related to, acquiring mission-representative imaging data from a small fixed-wing UAV, acting as a surrogate planetary aerial explorer. This question of successfully performing aerial explorer surveys is also tied to other topics of technical investigation, including the development of unique bio-inspired technologies.

Simple preparation of plant epidermal tissue for laser microdissection and downstream quantitative proteome and carbohydrate analysis

PubMed Central

Falter, Christian; Ellinger, Dorothea; von Hülsen, Behrend; Heim, René; Voigt, Christian A.

2015-01-01

The outwardly directed cell wall and associated plasma membrane of epidermal cells represent the first layers of plant defense against intruding pathogens. Cell wall modifications and the formation of defense structures at sites of attempted pathogen penetration are decisive for plant defense. A precise isolation of these stress-induced structures would allow a specific analysis of regulatory mechanism and cell wall adaption. However, methods for large-scale epidermal tissue preparation from the model plant Arabidopsis thaliana, which would allow proteome and cell wall analysis of complete, laser-microdissected epidermal defense structures, have not been provided. We developed the adhesive tape – liquid cover glass technique (ACT) for simple leaf epidermis preparation from A. thaliana, which is also applicable on grass leaves. This method is compatible with subsequent staining techniques to visualize stress-related cell wall structures, which were precisely isolated from the epidermal tissue layer by laser microdissection (LM) coupled to laser pressure catapulting. We successfully demonstrated that these specific epidermal tissue samples could be used for quantitative downstream proteome and cell wall analysis. The development of the ACT for simple leaf epidermis preparation and the compatibility to LM and downstream quantitative analysis opens new possibilities in the precise examination of stress- and pathogen-related cell wall structures in epidermal cells. Because the developed tissue processing is also applicable on A. thaliana, well-established, model pathosystems that include the interaction with powdery mildews can be studied to determine principal regulatory mechanisms in plant–microbe interaction with their potential outreach into crop breeding. PMID:25870605

Evaluation of two-dimensional electrophoresis and liquid chromatography – tandem mass spectrometry for tissue-specific protein profiling of laser-microdissected plant samples

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schad, Martina; Lipton, Mary S.; Giavalisco, Patrick

2005-07-14

Laser microdissection (LM) allows the collection of homogeneous tissue- and cell specific plant samples. The employment of this technique with subsequent protein analysis has thus far not been reported for plant tissues, probably due to the difficulties associated with defining a reasonable cellular morphology and, in parallel, allowing efficient protein extraction from tissue samples. The relatively large sample amount needed for successful proteome analysis is an additional issue that complicates protein profiling on a tissue- or even cell-specific level. In contrast to transcript profiling that can be performed from very small sample amounts due to efficient amplification strategies, there ismore » as yet no amplification procedure for proteins available. In the current study, we compared different tissue preparation techniques prior to LM/laser pressure catapulting (LMPC) with respect to their suitability for protein retrieval. Cryosectioning was identified as the best compromise between tissue morphology and effective protein extraction. After collection of vascular bundles from Arabidopsis thaliana stem tissue by LMPC, proteins were extracted and subjected to protein analysis, either by classical two-dimensional gel electrophoresis (2-DE), or by high-efficiency liquid chromatography (LC) in conjunction with tandem mass spectrometry (MS/MS). Our results demonstrate that both methods can be used with LMPC collected plant material. But because of the significantly lower sample amount required for LC-MS/MS than for 2-DE, the combination of LMPC and LC-MS/MS has a higher potential to promote comprehensive proteome analysis of specific plant tissues.« less

Career Profile- Jim Ross, Aerial Photographer

NASA Image and Video Library

2016-12-21

Check out what it takes to “capture the moment” at Mach speeds. The stunning aerial imagery of NASA Armstrong Flight Research Center comes from well-skilled photographers like Jim Ross, Photo Lead. This career profile video highlights Jim’s job responsibilities in documenting aircraft hardware installations, aerial research, and mission work that happens both on center and around the world. During Jim’s 27-year career, he has logged over 800 flight hours in twelve different types of aircraft.

Soil bacteria confer plant salt tolerance by tissue-specific regulation of the sodium transporter HKT1.

PubMed

Zhang, Huiming; Kim, Mi-Seong; Sun, Yan; Dowd, Scot E; Shi, Huazhong; Paré, Paul W

2008-06-01

Elevated sodium (Na(+)) decreases plant growth and, thereby, agricultural productivity. The ion transporter high-affinity K(+) transporter (HKT)1 controls Na(+) import in roots, yet dysfunction or overexpression of HKT1 fails to increase salt tolerance, raising questions as to HKT1's role in regulating Na(+) homeostasis. Here, we report that tissue-specific regulation of HKT1 by the soil bacterium Bacillus subtilis GB03 confers salt tolerance in Arabidopsis thaliana. Under salt stress (100 mM NaCl), GB03 concurrently down- and upregulates HKT1 expression in roots and shoots, respectively, resulting in lower Na(+) accumulation throughout the plant compared with controls. Consistent with HKT1 participation in GB03-induced salt tolerance, GB03 fails to rescue salt-stressed athkt1 mutants from stunted foliar growth and elevated total Na(+) whereas salt-stressed Na(+) export mutants sos3 show GB03-induced salt tolerance with enhanced shoot and root growth as well as reduced total Na(+). These results demonstrate that tissue-specific regulation of HKT1 is critical for managing Na(+) homeostasis in salt-stressed plants, as well as underscore the breadth and sophistication of plant-microbe interactions.

Arachnid aloft: directed aerial descent in neotropical canopy spiders.

PubMed

Yanoviak, Stephen P; Munk, Yonatan; Dudley, Robert

2015-09-06

The behaviour of directed aerial descent has been described for numerous taxa of wingless hexapods as they fall from the tropical rainforest canopy, but is not known in other terrestrial arthropods. Here, we describe similar controlled aerial behaviours for large arboreal spiders in the genus Selenops (Selenopidae). We dropped 59 such spiders from either canopy platforms or tree crowns in Panama and Peru; the majority (93%) directed their aerial trajectories towards and then landed upon nearby tree trunks. Following initial dorsoventral righting when necessary, falling spiders oriented themselves and then translated head-first towards targets; directional changes were correlated with bilaterally asymmetric motions of the anterolaterally extended forelegs. Aerial performance (i.e. the glide index) decreased with increasing body mass and wing loading, but not with projected surface area of the spider. Along with the occurrence of directed aerial descent in ants, jumping bristletails, and other wingless hexapods, this discovery of targeted gliding in selenopid spiders further indicates strong selective pressures against uncontrolled falls into the understory for arboreal taxa. © 2015 The Author(s).

Species and tissue type regulate long-term decomposition of brackish marsh plants grown under elevated CO2 conditions

USGS Publications Warehouse

Jones, Joshua A; Cherry, Julia A; Mckee, Karen L.

2016-01-01

Organic matter accumulation, the net effect of plant production and decomposition, contributes to vertical soil accretion in coastal wetlands, thereby playing a key role in whether they keep pace with sea-level rise. Any factor that affects decomposition may affect wetland accretion, including atmospheric CO2 concentrations. Higher CO2 can influence decomposition rates by altering plant tissue chemistry or by causing shifts in plant species composition or biomass partitioning. A combined greenhouse-field experiment examined how elevated CO2 affected plant tissue chemistry and subsequent decomposition of above- and belowground tissues of two common brackish marsh species, Schoenoplectus americanus (C3) and Spartina patens (C4). Both species were grown in monoculture and in mixture under ambient (350-385 μL L-1) or elevated (ambient + 300 μL L-1) atmospheric CO2 conditions, with all other growth conditions held constant, for one growing season. Above- and belowground tissues produced under these treatments were decomposed under ambient field conditions in a brackish marsh in the Mississippi River Delta, USA. Elevated CO2 significantly reduced nitrogen content of S. americanus, but not sufficiently to affect subsequent decomposition. Instead, long-term decomposition (percent mass remaining after 280 d) was controlled by species composition and tissue type. Shoots of S. patens had more mass remaining (41 ± 2%) than those of S. americanus (12 ± 2 %). Belowground material decomposed more slowly than that placed aboveground (62 ± 1% vs. 23 ± 3% mass remaining), but rates belowground did not differ between species. Increases in atmospheric CO2concentration will likely have a greater effect on overall decomposition in this brackish marsh community through shifts in species dominance or biomass allocation than through effects on tissue chemistry. Consequent changes in organic matter accumulation may alter marsh capacity to accommodate sea-level rise

Soil DIC uptake and fixation in Pinus taeda seedlings and its C contribution to plant tissues and ectomycorrhizal fungi

Treesearch

Chelcy R. Ford; Nina Wurzburger; Ronald L. Henderick; Robert O. Teskey

2007-01-01

Plants can aquaire carbon from sources other than atmospheric carbon dioxide (CO2), including soil-dissolved inorganic carbon (DIC). Although the next flux of CO2 is out of the root, soil DIC can be taken up by the root, transported within the plant, and fixed either photosynthetically or anaplerotically by plant tissues....

Changes in fatty acid composition in plant tissues expressing a mammalian delta9 desaturase.

PubMed

Moon, H; Hazebroek, J; Hildebrand, D F

2000-05-01

Plant tissues expressing a mammalian stearoyl-CoA delta9 desaturase were reported to accumulate delta9 hexadecenoic acid (16:1), normally very minor in most plant tissues. The transgenic plants were thoroughly analyzed for alterations of individual lipids in different subcellular sites. Western blot analysis indicated that the animal desaturase was targeted to the microsomes. The delta9 16:1 was incorporated into both the sn-1 and sn-2 positions of all the major membrane lipids tested, indicating that the endoplasmic reticulum acyltransferases do not exclude unsaturated C16 fatty acids from the sn-2 position. In addition to increases in monounsaturated and decreases in saturated fatty acids, accumulation of 16:1 was accompanied by a reduction in 18:3 in all the lipids tested except phosphatidylglycerol, and increases in 18:2 in phospholipids. Total C16 fatty acid content in the galactolipids of the transgenics was significantly higher than that in the control, but those in the phospholipids were unchanged. In transgenics, delta11 18:1 was detected in the sn-1 position of the lipids tested except phosphatidylinositol and phosphatidylserine. Introduction of the animal desaturase, controlled by a seed-specific phaseolin promoter, into soybean somatic embryo resulted in a significant reduction in saturated fatty acids. Such effects were greater in cotyledons than hypocotyl-radicles. This study demonstrated that the animal desaturase can be used to decrease the levels of saturated fatty acids in a crop plant.

Shoot atmospheric contact is of little importance to aeration of deeper portions of the wetland plant Meionectes brownii; submerged organs mainly acquire O2 from the water column or produce it endogenously in underwater photosynthesis.

PubMed

Rich, Sarah Meghan; Pedersen, Ole; Ludwig, Martha; Colmer, Timothy David

2013-01-01

Partial shoot submergence is considered less stressful than complete submergence of plants, as aerial contact allows gas exchange with the atmosphere. In situ microelectrode studies of the wetland plant Meionectes brownii showed that O(2) dynamics in the submerged stems and aquatic roots of partially submerged plants were similar to those of completely submerged plants, with internal O(2) concentrations in both organs dropping to less than 5 kPa by dawn regardless of submergence level. The anatomy at the nodes and the relationship between tissue porosity and rates of O(2) diffusion through stems were studied. Stem internodes contained aerenchyma and had mean gas space area of 17.7% per cross section, whereas nodes had 8.2%, but nodal porosity was highly variable, some nodes had very low porosity or were completely occluded (ca. 23% of nodes sampled). The cumulative effect of these low porosity nodes would have impeded internal O(2) movement down stems. Therefore, regardless of the presence of an aerial connection, the deeper portions of submerged organs sourced most of their O(2) via inwards diffusion from the water column during the night, and endogenous production in underwater photosynthesis during the daytime. © 2012 Blackwell Publishing Ltd.

The Impact of Microfibril Orientations on the Biomechanics of Plant Cell Walls and Tissues.

PubMed

Ptashnyk, Mariya; Seguin, Brian

2016-11-01

The microscopic structure and anisotropy of plant cell walls greatly influence the mechanical properties, morphogenesis, and growth of plant cells and tissues. The microscopic structure and properties of cell walls are determined by the orientation and mechanical properties of the cellulose microfibrils and the mechanical properties of the cell wall matrix. Viewing the shape of a plant cell as a square prism with the axis aligning with the primary direction of expansion and growth, the orientation of the microfibrils within the side walls, i.e. the parts of the cell walls on the sides of the cells, is known. However, not much is known about their orientation at the upper and lower ends of the cell. Here we investigate the impact of the orientation of cellulose microfibrils within the upper and lower parts of the plant cell walls by solving the equations of linear elasticity numerically. Three different scenarios for the orientation of the microfibrils are considered. We also distinguish between the microstructure in the side walls given by microfibrils perpendicular to the main direction of the expansion and the situation where the microfibrils are rotated through the wall thickness. The macroscopic elastic properties of the cell wall are obtained using homogenization theory from the microscopic description of the elastic properties of the cell wall microfibrils and wall matrix. It is found that the orientation of the microfibrils in the upper and lower parts of the cell walls affects the expansion of the cell in the lateral directions and is particularly important in the case of forces acting on plant cell walls and tissues.

An RNA isolation system for plant tissues rich in secondary metabolites

PubMed Central

2011-01-01

Background Secondary metabolites are reported to interfere with the isolation of RNA particularly with the recipes that use guanidinium-based salt. Such interference was observed in isolation of RNA with medicinal plants rheum (Rheum australe) and arnebia (Arnebia euchroma). A rapid and less cumbersome system for isolation of RNA was essential to facilitate any study related to gene expression. Findings An RNA isolation system free of guanidinium salt was developed that successfully isolated RNA from rheum and arnebia. The method took about 45 min and was successfully evaluated on twenty one tissues with varied secondary metabolites. The A260/280 ratio ranged between 1.8 - 2.0 with distinct 28 S and 18 S rRNA bands visible on a formaldehyde-agarose gel. Conclusions The present manuscript describes a rapid protocol for isolation of RNA, which works well with all the tissues examined so far. The remarkable feature was the success in isolation of RNA with those tissues, wherein the most commonly used methods failed. Isolated RNA was amenable to downstream applications such as reverse transcription-polymerase chain reaction (RT-PCR), differential display (DD), suppression subtractive hybridization (SSH) library construction, and northern hybridization. PMID:21443767

Noise from aerial bursts of fireworks

NASA Technical Reports Server (NTRS)

Maglieri, D. J.; Henderson, H. R.

1973-01-01

A study was made recording the pressure time histories of the aerial bursts of mortars of various sizes launched during an actual fireworks display. The peak overpressure and duration of blast noise as well as the energy spectral density are compared with the characteristics of a blasting cap and of an F-104 aircraft at a Mach number of 1.4 and an altitude of 42,000 ft. Noise levels of the fireworks aerial bursts peaked 15 decibels below levels deemed damaging to hearing.

Resource understanding: a challenge to aerial methods

USGS Publications Warehouse

Udall, Stewart L.

1965-01-01

Aerial survey methods are speeding acquisition of survey data needed to provide and manage the nation's resources. These methods have been applied to topographic mapping for a number of years and the record clearly shows their advantages in terms of cost and speed in contrast to the ground methods that have been historically employed. Limited use is now being made of aerial methods to assist cadastral surveys, in location, acquisition and development of National Parks, in mapping the geology of the nation, in locating and developing water resources, and in surveys of the oceans. It is the purpose of this paper to call attention to these uses and to encourage the scientific community to further refine aerial methods so that their use may be increased and the veracity of data improved.

Melon yield prediction using small unmanned aerial vehicles

NASA Astrophysics Data System (ADS)

Zhao, Tiebiao; Wang, Zhongdao; Yang, Qi; Chen, YangQuan

2017-05-01

Thanks to the development of camera technologies, small unmanned aerial systems (sUAS), it is possible to collect aerial images of field with more flexible visit, higher resolution and much lower cost. Furthermore, the performance of objection detection based on deeply trained convolutional neural networks (CNNs) has been improved significantly. In this study, we applied these technologies in the melon production, where high-resolution aerial images were used to count melons in the field and predict the yield. CNN-based object detection framework-Faster R-CNN is applied in the melon classification. Our results showed that sUAS plus CNNs were able to detect melons accurately in the late harvest season.

Distributions of imidacloprid, imidacloprid-olefin and imidacloprid-urea in green plant tissues and roots of rapeseed (Brassica napus) from artificially contaminated potting soil.

PubMed

Seifrtova, Marcela; Halesova, Tatana; Sulcova, Klara; Riddellova, Katerina; Erban, Tomas

2017-05-01

Imidacloprid-urea is the primary imidacloprid soil metabolite, whereas imidacloprid-olefin is the main plant-relevant metabolite and is more toxic to insects than imidacloprid. We artificially contaminated potting soil and used quantitative UHPLC-QqQ-MS/MS to determine the imidacloprid, imidacloprid-olefin and imidacloprid-urea distributions in rapeseed green plant tissues and roots after 4 weeks of exposure. In soil, the imidacloprid/imidacloprid-urea molar ratios decreased similarly after the 250 and 2500 µg kg -1 imidacloprid treatments. The imidacloprid/imidacloprid-urea molar ratios in the root and soil were similar, whereas in the green plant tissue, imidacloprid-urea increased more than twofold compared with the root. Although imidacloprid-olefin was prevalent in the green plant tissues, with imidacloprid/imidacloprid-olefin molar ratios of 2.24 and 1.47 for the 250 and 2500 µg kg -1 treatments respectively, it was not detected in the root. However, imidacloprid-olefin was detected in the soil after the 2500 µg kg -1 imidacloprid treatment. Significant proportions of imidacloprid-olefin and imidacloprid-urea in green plant tissues were demonstrated. The greater imidacloprid supply increased the imidacloprid-olefin/imidacloprid molar ratio in the green plant tissues. The absence of imidacloprid-olefin in the root excluded its retransport from leaves. The similar imidacloprid/imidacloprid-urea ratios in the soil and root indicated that the root serves primarily for transporting these substances. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

SITE I - AERIAL - MSC

NASA Image and Video Library

1966-07-01

S66-42379 (1966) --- Aerial view of construction progress at the Manned Spacecraft Center, Houston, Texas. NOTE: The Manned Spacecraft Center was named Lyndon B. Johnson Space Center in memory of the late President following his death.

The relationship between growth of the aerial part and alkaloid content variation in cultivated Aconitum carmichaeli Debeaux.

PubMed

Kawasaki, Ryoichi; Motoya, Wakako; Atsumi, Toshiyuki; Mouri, Chika; Kakiuchi, Nobuko; Mikage, Masayuki

2011-01-01

Processed root of aconite, Aconitum carmichaeli Debeaux--known as bushi in Japan--is indispensable for treating diseases among elderly persons in Japanese and Chinese traditional medicine. Its active component is bushi diester alkaloid (BDA), which consists of aconitine (ACO), mesaconitine (MES), hypaconitine (HYP), and jesaconitine (JES). Since an overdose of BDA results in severe side effects, the BDA content should be within safe limits. However, the BDA content of raw aconite root, even that produced by standard cultivation procedures, varies greatly. In this study, to clarify the cause of BDA variation, we examined the weight and BDA content of each part of cultivated A. carmichaeli: the aerial part, the mother tuberous root (MT), the daughter tuberous root (DT), and the rootlet (RL). We found the following positive relationships: between aerial part weight and DT weight, aerial part weight and BDA content in stem of apex, and BDA content in stem of apex and total BDA of DT attached to the plant. Furthermore, DT belonging to a higher weight group showed less BDA content variation. In addition, BDA of DT and those of MT and RL differ in both content and composition. In conclusion, it was suggested that the weight or the size of the aerial part was a good marker for monitoring BDA content and its variation in the tuberous root, and it was found to be desirable to prevent mixing MT and RL at harvest.

Early diagenesis of vascular plant tissues: Lignin and cutin decomposition and biogeochemical implications

NASA Astrophysics Data System (ADS)

Opsahl, Stephen; Benner, Ronald

1995-12-01

Long-term subaqueous decomposition patterns of five different vascular plant tissues including mangrove leaves and wood ( Avicennia germinans), cypress needles and wood ( Taxodium distichum) and smooth cordgrass ( Spartina alternifora) were followed for a period of 4.0 years, representing the longest litter bag decomposition study to date. All tissues decomposed under identical conditions and final mass losses were 97, 68, 86, 39, and 93%, respectively. Analysis of the lignin component of herbaceous tissues using alkaline CuO oxidation was complicated by the presence of a substantial ester-bound phenol component composed primarily of cinnamyl phenols. To overcome this problem, we introduce a new parameter to represent lignin, Λ6. Λ6 is comprised only of the six syringyl and vanillyl phenols and was found to be much less sensitive to diagenetic variation than the commonly used parameter Λ, which includes the cinnamyl phenols. Patterns of change in lignin content were strongly dependent on tissue type, ranging from 77% enrichment in smooth cordgrass to 6% depletion in cypress needles. In contrast, depletion of cutin was extensive (65-99%) in all herbaceous tissues. Despite these differences in the overall reactivity of lignin and cutin, both macromolecules were extensively degraded during the decomposition period. The long-term decomposition series also provided very useful information about the compositional parameters which are derived from the specific oxidation products of both lignin and cutin. The relative lability of ester-bound cinnamyl phenols compromised their use in parameters to distinguish woody from herbaceous plant debris. The dimer to monomer ratios of lignin-derived phenols indicated that most intermonomeric linkages in lignin degraded at similar rates. Acid to aldehyde ratios of vanillyl and syringyl phenols became elevated, particularly during the latter stages of decomposition supporting the use of these parameters as indicators of diagenetic

Deep sequencing analysis of the transcriptomes of peanut aerial and subterranean young pods identifies candidate genes related to early embryo abortion.

PubMed

Chen, Xiaoping; Zhu, Wei; Azam, Sarwar; Li, Heying; Zhu, Fanghe; Li, Haifen; Hong, Yanbin; Liu, Haiyan; Zhang, Erhua; Wu, Hong; Yu, Shanlin; Zhou, Guiyuan; Li, Shaoxiong; Zhong, Ni; Wen, Shijie; Li, Xingyu; Knapp, Steve J; Ozias-Akins, Peggy; Varshney, Rajeev K; Liang, Xuanqiang

2013-01-01

The failure of peg penetration into the soil leads to seed abortion in peanut. Knowledge of genes involved in these processes is comparatively deficient. Here, we used RNA-seq to gain insights into transcriptomes of aerial and subterranean pods. More than 2 million transcript reads with an average length of 396 bp were generated from one aerial (AP) and two subterranean (SP1 and SP2) pod libraries using pyrosequencing technology. After assembly, sets of 49 632, 49 952 and 50 494 from a total of 74 974 transcript assembly contigs (TACs) were identified in AP, SP1 and SP2, respectively. A clear linear relationship in the gene expression level was observed between these data sets. In brief, 2194 differentially expressed TACs with a 99.0% true-positive rate were identified, among which 859 and 1068 TACs were up-regulated in aerial and subterranean pods, respectively. Functional analysis showed that putative function based on similarity with proteins catalogued in UniProt and gene ontology term classification could be determined for 59 342 (79.2%) and 42 955 (57.3%) TACs, respectively. A total of 2968 TACs were mapped to 174 KEGG pathways, of which 168 were shared by aerial and subterranean transcriptomes. TACs involved in photosynthesis were significantly up-regulated and enriched in the aerial pod. In addition, two senescence-associated genes were identified as significantly up-regulated in the aerial pod, which potentially contribute to embryo abortion in aerial pods, and in turn, to cessation of swelling. The data set generated in this study provides evidence for some functional genes as robust candidates underlying aerial and subterranean pod development and contributes to an elucidation of the evolutionary implications resulting from fruit development under light and dark conditions. © 2012 The Authors Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.

Ground-Cover Measurements: Assessing Correlation Among Aerial and Ground-Based Methods

NASA Astrophysics Data System (ADS)

Booth, D. Terrance; Cox, Samuel E.; Meikle, Tim; Zuuring, Hans R.

2008-12-01

Wyoming’s Green Mountain Common Allotment is public land providing livestock forage, wildlife habitat, and unfenced solitude, amid other ecological services. It is also the center of ongoing debate over USDI Bureau of Land Management’s (BLM) adjudication of land uses. Monitoring resource use is a BLM responsibility, but conventional monitoring is inadequate for the vast areas encompassed in this and other public-land units. New monitoring methods are needed that will reduce monitoring costs. An understanding of data-set relationships among old and new methods is also needed. This study compared two conventional methods with two remote sensing methods using images captured from two meters and 100 meters above ground level from a camera stand (a ground, image-based method) and a light airplane (an aerial, image-based method). Image analysis used SamplePoint or VegMeasure software. Aerial methods allowed for increased sampling intensity at low cost relative to the time and travel required by ground methods. Costs to acquire the aerial imagery and measure ground cover on 162 aerial samples representing 9000 ha were less than 3000. The four highest correlations among data sets for bare ground—the ground-cover characteristic yielding the highest correlations (r)—ranged from 0.76 to 0.85 and included ground with ground, ground with aerial, and aerial with aerial data-set associations. We conclude that our aerial surveys are a cost-effective monitoring method, that ground with aerial data-set correlations can be equal to, or greater than those among ground-based data sets, and that bare ground should continue to be investigated and tested for use as a key indicator of rangeland health.

Flavonoids from the aerial parts of Houttuynia cordata attenuate lung inflammation in mice.

PubMed

Lee, Ju Hee; Ahn, Jongmin; Kim, Jin Woong; Lee, Sang Gook; Kim, Hyun Pyo

2015-07-01

The aerial parts of Houttuynia cordata used for treating inflammation-related disorders contain flavonoids as major constituents. Since certain flavonoids possess anti-inflammatory activity, especially in the lung, the pharmacological activities of H. cordata and the flavonoid constituents were evaluated using in vitro and in vivo models of lung inflammation. The 70 % ethanol extract of the aerial parts of H. cordata inhibited the production of inflammatory biomarkers IL-6 and NO in lung epithelial cells (A549) and alveolar macrophages (MH-S), respectively. And the same plant material, administered orally (100 and 400 mg/kg), significantly inhibited lung inflammatory response in a mouse model of lipopolysaccharide (LPS)-induced acute lung injury. From the extract, major flavonoids including afzelin, hyperoside and quercitrin were successfully isolated and they also attenuated LPS-induced lung inflammation in mice by oral administration. In particular, quercitrin showed most potent activity at 100 mg/kg. These results demonstrate for the first time that H. cordata and three flavonoid constituents have a therapeutic potential for treating lung inflammatory disorders.

SITE I - AERIAL - MSC

NASA Image and Video Library

1965-08-01

S65-51530 (September 1965) --- Aerial view of Manned Spacecraft Center, Site 1, Houston, Texas, looking north. NOTE: The Manned Spacecraft Center was named Lyndon B. Johnson Space Center in memory of the late President following his death.

Advanced Image Processing of Aerial Imagery

NASA Technical Reports Server (NTRS)

Woodell, Glenn; Jobson, Daniel J.; Rahman, Zia-ur; Hines, Glenn

2006-01-01

Aerial imagery of the Earth is an invaluable tool for the assessment of ground features, especially during times of disaster. Researchers at the NASA Langley Research Center have developed techniques which have proven to be useful for such imagery. Aerial imagery from various sources, including Langley's Boeing 757 Aries aircraft, has been studied extensively. This paper discusses these studies and demonstrates that better-than-observer imagery can be obtained even when visibility is severely compromised. A real-time, multi-spectral experimental system will be described and numerous examples will be shown.

AERIAL OF SHUTTLE LANDING FACILITY [SLF] POURING OF CONCRETE

NASA Technical Reports Server (NTRS)

1975-01-01

AERIAL OF SHUTTLE LANDING FACILITY [SLF] POURING OF CONCRETE KSC-375C-10036.31 108-KSC-375C-10036.31, P-21426, ARCHIVE-04502 Aerial oblique of Shuttle runway facilities. Pouring concrete on runway. Direction north - altitude 100'.

Ecological Energetics of an Abundant Aerial Insectivore, the Purple Martin

PubMed Central

Kelly, Jeffrey F.; Bridge, Eli S.; Frick, Winifred F.; Chilson, Phillip B.

2013-01-01

The atmospheric boundary layer and lower free atmosphere, or aerosphere, is increasingly important for human transportation, communication, environmental monitoring, and energy production. The impacts of anthropogenic encroachment into aerial habitats are not well understood. Insectivorous birds and bats are inherently valuable components of biodiversity and play an integral role in aerial trophic dynamics. Many of these insectivores are experiencing range-wide population declines. As a first step toward gaging the potential impacts of these declines on the aerosphere’s trophic system, estimates of the biomass and energy consumed by aerial insectivores are needed. We developed a suite of energetics models for one of the largest and most common avian aerial insectivores in North America, the Purple Martin ( Progne subis ). The base model estimated that Purple Martins consumed 412 (± 104) billion insects*y-1 with a biomass of 115,860 (± 29,192) metric tonnes*y-1. During the breeding season Purple Martins consume 10.3 (+ 3.0) kg of prey biomass per km3 of aerial habitat, equal to about 36,000 individual insects*km-3. Based on these calculations, the cumulative seasonal consumption of insects*km-3 is greater in North America during the breeding season than during other phases of the annual cycle, however the maximum daily insect consumption*km-3 occurs during fall migration. This analysis provides the first range-wide quantitative estimate of the magnitude of the trophic impact of this large and common aerial insectivore. Future studies could use a similar modeling approach to estimate impacts of the entire guild of aerial insectivores at a variety of temporal and spatial scales. These analyses would inform our understanding of the impact of population declines among aerial insectivores on the aerosphere’s trophic dynamics. PMID:24086755

Laser assisted microdissection, an efficient technique to understand tissue specific gene expression patterns and functional genomics in plants.

PubMed

Gautam, Vibhav; Sarkar, Ananda K

2015-04-01

Laser assisted microdissection (LAM) is an advanced technology used to perform tissue or cell-specific expression profiling of genes and proteins, owing to its ability to isolate the desired tissue or cell type from a heterogeneous population. Due to the specificity and high efficiency acquired during its pioneering use in medical science, the LAM technique has quickly been adopted for use in many biological researches. Today, it has become a potent tool to address a wide range of questions in diverse field of plant biology. Beginning with comparative transcriptome analysis of different tissues such as reproductive parts, meristems, lateral organs, roots etc., LAM has also been extensively used in plant-pathogen interaction studies, proteomics, and metabolomics. In combination with next generation sequencing and proteomics analysis, LAM has opened up promising opportunities in the area of large scale functional studies in plants. Ever since the advent of this technique, significant improvements have been achieved in term of its instrumentation and method, which has made LAM a more efficient tool applicable in wider research areas. Here, we discuss the advancement of LAM technique with special emphasis on its methodology and highlight its scope in modern research areas of plant biology. Although we put emphasis on use of LAM in transcriptome studies, which is mostly used, we also discuss its recent application and scope in proteome and metabolome studies.

Overview of meteorological measurements for aerial spray modeling.

PubMed

Rafferty, J E; Biltoft, C A; Bowers, J F

1996-06-01

The routine meteorological observations made by the National Weather Service have a spatial resolution on the order of 1,000 km, whereas the resolution needed to conduct or model aerial spray applications is on the order of 1-10 km. Routinely available observations also do not include the detailed information on the turbulence and thermal structure of the boundary layer that is needed to predict the transport, dispersion, and deposition of aerial spray releases. This paper provides an overview of the information needed to develop the meteorological inputs for an aerial spray model such as the FSCBG and discusses the different types of instruments that are available to make the necessary measurements.

CONTEXTUAL AERIAL VIEW OF "COLD" NORTH HALF OF MTR COMPLEX. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

CONTEXTUAL AERIAL VIEW OF "COLD" NORTH HALF OF MTR COMPLEX. CAMERA FACING EASTERLY. FOREGROUND CORNER CONTAINS OIL STORAGE TANKS. WATER TANKS AND WELL HOUSES ARE BEYOND THEM TO THE LEFT. LARGE LIGHT-COLORED BUILDING IN CENTER OF VIEW IS STEAM PLANT. DEMINERALIZER AND WATER STORAGE TANK ARE BEYOND. SIX-CELL COOLING TOWER AND ITS PUMP HOUSE ARE ABOVE IT IN VIEW. SERVICE BUILDINGS INCLUDING CANTEEN ARE ON NORTH SIDE OF ROAD. "EXCLUSION" AREA IS BEYOND ROAD. COMPARE LOCATION OF EXCLUSION-AREA GATE WITH PHOTO ID-33-G-202. INL NEGATIVE NO. 3608. Unknown Photographer, 10/30/1951 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

Advancements in mass spectrometry for biological samples: Protein chemical cross-linking and metabolite analysis of plant tissues

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klein, Adam

2015-01-01

This thesis presents work on advancements and applications of methodology for the analysis of biological samples using mass spectrometry. Included in this work are improvements to chemical cross-linking mass spectrometry (CXMS) for the study of protein structures and mass spectrometry imaging and quantitative analysis to study plant metabolites. Applications include using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to further explore metabolic heterogeneity in plant tissues and chemical interactions at the interface between plants and pests. Additional work was focused on developing liquid chromatography-mass spectrometry (LC-MS) methods to investigate metabolites associated with plant-pest interactions.

Aerial spray technology: possibilities and limitations for control of pear thrips

Treesearch

Karl Mierzejewski

1991-01-01

The feasibility of using aerial application as a means of managing a pear thrips infestation in maple forest stands is examined, based on existing knowledge of forest aerial application acquired from theoretical and empirical studies. Specific strategies by which aerial application should be performed and potential problem areas are discussed. Two new tools, aircraft...

Acquisition and evolution of plant pathogenesis-associated gene clusters and candidate determinants of tissue-specificity in xanthomonas.

PubMed

Lu, Hong; Patil, Prabhu; Van Sluys, Marie-Anne; White, Frank F; Ryan, Robert P; Dow, J Maxwell; Rabinowicz, Pablo; Salzberg, Steven L; Leach, Jan E; Sonti, Ramesh; Brendel, Volker; Bogdanove, Adam J

2008-01-01

Xanthomonas is a large genus of plant-associated and plant-pathogenic bacteria. Collectively, members cause diseases on over 392 plant species. Individually, they exhibit marked host- and tissue-specificity. The determinants of this specificity are unknown. To assess potential contributions to host- and tissue-specificity, pathogenesis-associated gene clusters were compared across genomes of eight Xanthomonas strains representing vascular or non-vascular pathogens of rice, brassicas, pepper and tomato, and citrus. The gum cluster for extracellular polysaccharide is conserved except for gumN and sequences downstream. The xcs and xps clusters for type II secretion are conserved, except in the rice pathogens, in which xcs is missing. In the otherwise conserved hrp cluster, sequences flanking the core genes for type III secretion vary with respect to insertion sequence element and putative effector gene content. Variation at the rpf (regulation of pathogenicity factors) cluster is more pronounced, though genes with established functional relevance are conserved. A cluster for synthesis of lipopolysaccharide varies highly, suggesting multiple horizontal gene transfers and reassortments, but this variation does not correlate with host- or tissue-specificity. Phylogenetic trees based on amino acid alignments of gum, xps, xcs, hrp, and rpf cluster products generally reflect strain phylogeny. However, amino acid residues at four positions correlate with tissue specificity, revealing hpaA and xpsD as candidate determinants. Examination of genome sequences of xanthomonads Xylella fastidiosa and Stenotrophomonas maltophilia revealed that the hrp, gum, and xcs clusters are recent acquisitions in the Xanthomonas lineage. Our results provide insight into the ancestral Xanthomonas genome and indicate that differentiation with respect to host- and tissue-specificity involved not major modifications or wholesale exchange of clusters, but subtle changes in a small number of genes or

Potential of Chilopsis Linearis for Gold Phytomining: Using XAS to Determine Gold Reduction And Nanoparticle Formation Within Plant Tissues

DOE Office of Scientific and Technical Information (OSTI.GOV)

E, Rodriguez; Parsons, J.G.; Peralta-Videa, J.R.

This study reports on the capability of the desert plant Chilopsis linearis (Cav.) Sweet (desert willow) to uptake gold (Au) from gold-enriched media at different plant-growth stages. Plants were exposed to 20, 40, 80, 160, and 320 mg Au L{sup -1} in agar-based growing media for 13, 18, 23, and 35 d. The Au content and oxidation state of Au in the plants were determined using an inductively coupled plasma/optical emission spectrometer (ICP/OES) and X-ray absorption spectroscopy (XAS), respectively. Gold concentrations ranging from 20 to 80 mg Au L{sup -1} did not significantly affect Chilopsis linearis plant growth. The concentrationmore » of gold in the plants increased as the age of the plant increased. The Au concentrations in leaves for the 20, 40, 80, and 160 mg Au L{sup -1} treatments were 32, 60, 62, and 179 mg Au kg{sup -1} dry weight mass, respectively, demonstrating the gold uptake capability of desert willow. The XAS data indicated that desert willow produced gold nanoparticles within plant tissues. Plants exposed to 160 mg Au L{sup -1} formed nanoparticles that averaged approximately 8, 35, and 18 in root, stem, and leaves, respectively. It was observed that the average size of the Au nanoparticles formed by the plants is related to the total Au concentration in tissues and their location in the plant.« less

Pseudohypericin and hyperforin in Hypericum perforatum from Northern Turkey: variation among populations, plant parts and phenological stages.

PubMed

Cirak, Cüneyt; Radusiene, Jolita; Janulis, Valdimaras; Ivanauskas, Liudas

2008-05-01

Hypericum perforatum is a perennial medicinal plant known as "St. John's wort" in Western Europe and has been used in the treatment of several diseases for centuries. In the present study, morphologic, phenologic and population variability in pseudohypericin and hyperforin concentrations among H. perforatum populations from Northern Turkey was investigated for the first time. The aerial parts of H. perforatum plants representing a total of 30 individuals were collected at full flowering from 10 sites of Northern Turkey to search the regional variation in the secondary metabolite concentrations. For morphologic and phenologic sampling, plants from one site were gathered in five phenological stages: vegetative, floral budding, full flowering, fresh fruiting and mature fruiting. The plant materials were air-dried at room temperature and subsequently assayed for chemical concentrations by high performance liquid chromatography. Secondary metabolite concentrations ranged from traces to 2.94 mg/g dry weight (DW) for pseudohypericin and traces -6.29 mg/g DW for hyperforin. The differences in the secondary metabolite concentrations among populations of H. perforatum were found to be significant. The populations varied greatly in hyperforin concentrations, whereas they produced a similar amount of pseudohypericin. Concentrations of both secondary metabolites in all tissues increased with advancing of plant development and higher accumulation levels were reached at flowering. Among different tissues, full opened flowers were found to be superior to stems, leaves and the other reproductive parts with regard to pseudohypericin and hyperforin accumulations. The present findings might be useful to optimize the processing methodology of wild-harvested plant material and obtain increased concentrations of these secondary metabolites.

An algorithm for approximate rectification of digital aerial images

USDA-ARS?s Scientific Manuscript database

High-resolution aerial photography is one of the most valuable tools available for managing extensive landscapes. With recent advances in digital camera technology, computer hardware, and software, aerial photography is easier to collect, store, and transfer than ever before. Images can be automa...

Unmanned Aerial Vehicle Systems for Disaster Relief: Tornado Alley

NASA Technical Reports Server (NTRS)

DeBusk, Wesley M.

2009-01-01

Unmanned aerial vehicle systems are currently in limited use for public service missions worldwide. Development of civil unmanned technology in the United States currently lags behind military unmanned technology development in part because of unresolved regulatory and technological issues. Civil unmanned aerial vehicle systems have potential to augment disaster relief and emergency response efforts. Optimal design of aerial systems for such applications will lead to unmanned vehicles which provide maximum potentiality for relief and emergency response while accounting for public safety concerns and regulatory requirements. A case study is presented that demonstrates application of a civil unmanned system to a disaster relief mission with the intent on saving lives. The concept utilizes unmanned aircraft to obtain advanced warning and damage assessments for tornados and severe thunderstorms. Overview of a tornado watch mission architecture as well as commentary on risk, cost, need for, and design tradeoffs for unmanned aerial systems are provided.

Secondary Metabolites of Hypericum leptophyllum Hochst., an Endemic Turkish Species

PubMed Central

Camas, Necdet; Radusiene, Jolita; Stanius, Zydrunas; Caliskan, Omer; Cirak, Cuneyt

2012-01-01

In the present study, the presence of the phloroglucinol derivative hyperforin, the naphthodianthrones hypericin and pseudohypericin, the phenylpropane chlorogenic acid and the flavonoids rutin, hyperoside, kaempferol, isoquercetine, quercitrine, and quercetine was investigated in Hypericum leptophyllum Hochst., an endemic Turkish species for the first time. The aerial parts representing a total of 30 individuals were collected at full flowering and dissected into floral, leaf, and stem tissues. After being dried at room temperature, the plant materials were assayed for secondary metabolite concentrations by HPLC. Aerial plant parts accumulated chlorogenic acid, hyperoside, isoquercetine, quercitrine, and quercetine, but they did not accumulate hyperforin, hypericin, pseudohypericin, rutin, and kaempferol. Accumulation levels of the detected compounds varied with plant tissues. Such kind of data could be useful for elucidation of the chemotaxonomical significance of the corresponding compounds and phytochemical evaluation of this endemic species. PMID:22649295

Genes encoding novel lipid transporters and their use to increase oil production in vegetative tissues of plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, Changcheng; Fan, Jilian; Yan, Chengshi

The present invention discloses a novel gene encoding a transporter protein trigalactosyldiacylglycerol-5 (TGD5), mutations thereof and their use to enhance TAG production and retention in plant vegetative tissue.

Manipulation of host plant cells and tissues by gall-inducing insects and adaptive strategies used by different feeding guilds.

PubMed

Oliveira, D C; Isaias, R M S; Fernandes, G W; Ferreira, B G; Carneiro, R G S; Fuzaro, L

2016-01-01

Biologists who study insect-induced plant galls are faced with the overwhelming diversity of plant forms and insect species. A challenge is to find common themes amidst this diversity. We discuss common themes that have emerged from our cytological and histochemical studies of diverse neotropical insect-induced galls. Gall initiation begins with recognition of reactive plant tissues by gall inducers, with subsequent feeding and/or oviposition triggering a cascade of events. Besides, to induce the gall structure insects have to synchronize their life cycle with plant host phenology. We predict that reactive oxygen species (ROS) play a role in gall induction, development and histochemical gradient formation. Controlled levels of ROS mediate the accumulation of (poly)phenols, and phytohormones (such as auxin) at gall sites, which contributes to the new cell developmental pathways and biochemical alterations that lead to gall formation. The classical idea of an insect-induced gall is a chamber lined with a nutritive tissue that is occupied by an insect that directly harvests nutrients from nutritive cells via its mouthparts, which function mechanically and/or as a delivery system for salivary secretions. By studying diverse gall-inducing insects we have discovered that insects with needle-like sucking mouthparts may also induce a nutritive tissue, whose nutrients are indirectly harvested as the gall-inducing insects feeds on adjacent vascular tissues. Activity of carbohydrate-related enzymes across diverse galls corroborates this hypothesis. Our research points to the importance of cytological and histochemical studies for elucidating mechanisms of induced susceptibility and induced resistance. Copyright © 2015 Elsevier Ltd. All rights reserved.

Fast probing of glucose and fructose in plant tissues via plasmonic affinity sandwich assay with molecularly-imprinted extraction microprobes.

PubMed

Muhammad, Pir; Liu, Jia; Xing, Rongrong; Wen, Yanrong; Wang, Yijia; Liu, Zhen

2017-12-01

Determination of specific target compounds in agriculture food and natural plant products is essential for many purposes; however, it is often challenging due to the complexity of the sample matrices. Herein we present a new approach called plasmonic affinity sandwich assay for the facile and rapid probing of glucose and fructose in plant tissues. The approach mainly relies on molecularly imprinted plasmonic extraction microprobes, which were prepared on gold-coated acupuncture needles via boronate affinity controllable oriented surface imprinting with the target monosaccharide as the template molecules. An extraction microprobe was inserted into plant tissues under investigation, which allowed for the specific extraction of glucose or fructose from the tissues. The glucose or fructose molecules extracted on the microprobe were labeled with boronic acid-functionalized Raman-active silver nanoparticles, and thus affinity sandwich complexes were formed on the microprobes. After excess Raman nanotags were washed away, the microprobe was subjected to Raman detection. Upon being irradiated with a laser beam, surface plasmon on the gold-coated microprobes was generated, which further produced plasmon-enhanced Raman scattering of the silver-based nanotags and thereby provided sensitive detection. Apple fruits, which contain abundant glucose and fructose, were used as a model of plant tissues. The approach exhibited high specificity, good sensitivity (limit of detection, 1 μg mL -1 ), and fast speed (the whole procedure required only 20 min). The spatial distribution profiles of glucose and fructose within an apple were investigated by the developed approach. Copyright © 2017 Elsevier B.V. All rights reserved.

Aerially released spray penetration of a tall coniferous canopy

USDA-ARS?s Scientific Manuscript database

An aerial spray deposition project was designed to evaluate aerial application to an Eastern Hemlock (Tsuga canadensis) canopy to combat Hemlock Woolly Adelgid (Adelges tsugae). This adelgid offers a difficult target residing in the forest canopy at the nodes of branchlets. The study collected 1680 ...

Ectopic KNOX Expression Affects Plant Development by Altering Tissue Cell Polarity and Identity[OPEN

PubMed Central

Rebocho, Alexandra B.

2016-01-01

Plant development involves two polarity types: tissue cell (asymmetries within cells are coordinated across tissues) and regional (identities vary spatially across tissues) polarity. Both appear altered in the barley (Hordeum vulgare) Hooded mutant, in which ectopic expression of the KNOTTED1-like Homeobox (KNOX) gene, BKn3, causes inverted polarity of differentiated hairs and ectopic flowers, in addition to wing-shaped outgrowths. These lemma-specific effects allow the spatiotemporal analysis of events following ectopic BKn3 expression, determining the relationship between KNOXs, polarity, and shape. We show that tissue cell polarity, based on localization of the auxin transporter SISTER OF PINFORMED1 (SoPIN1), dynamically reorients as ectopic BKn3 expression increases. Concurrently, ectopic expression of the auxin importer LIKE AUX1 and boundary gene NO APICAL MERISTEM is activated. The polarity of hairs reflects SoPIN1 patterns, suggesting that tissue cell polarity underpins oriented cell differentiation. Wing cell files reveal an anisotropic growth pattern, and computational modeling shows how polarity guiding growth can account for this pattern and wing emergence. The inverted ectopic flower orientation does not correlate with SoPIN1, suggesting that this form of regional polarity is not controlled by tissue cell polarity. Overall, the results suggest that KNOXs trigger different morphogenetic effects through interplay between tissue cell polarity, identity, and growth. PMID:27553356

Vehicle detection in aerial surveillance using dynamic Bayesian networks.

PubMed

Cheng, Hsu-Yung; Weng, Chih-Chia; Chen, Yi-Ying

2012-04-01

We present an automatic vehicle detection system for aerial surveillance in this paper. In this system, we escape from the stereotype and existing frameworks of vehicle detection in aerial surveillance, which are either region based or sliding window based. We design a pixelwise classification method for vehicle detection. The novelty lies in the fact that, in spite of performing pixelwise classification, relations among neighboring pixels in a region are preserved in the feature extraction process. We consider features including vehicle colors and local features. For vehicle color extraction, we utilize a color transform to separate vehicle colors and nonvehicle colors effectively. For edge detection, we apply moment preserving to adjust the thresholds of the Canny edge detector automatically, which increases the adaptability and the accuracy for detection in various aerial images. Afterward, a dynamic Bayesian network (DBN) is constructed for the classification purpose. We convert regional local features into quantitative observations that can be referenced when applying pixelwise classification via DBN. Experiments were conducted on a wide variety of aerial videos. The results demonstrate flexibility and good generalization abilities of the proposed method on a challenging data set with aerial surveillance images taken at different heights and under different camera angles.

Laser Doppler velocimeter aerial spray measurements

NASA Technical Reports Server (NTRS)

Zalay, A. D.; Eberle, W. R.; Howle, R. E.; Shrider, K. R.

1978-01-01

An experimental research program for measuring the location, spatial extent, and relative concentration of airborne spray clouds generated by agricultural aircraft is described. The measurements were conducted with a ground-based laser Doppler velocimeter. The remote sensing instrumentation, experimental tests, and the results of the flight tests are discussed. The cross section of the aerial spray cloud and the observed location, extent, and relative concentration of the airborne particulates are presented. It is feasible to use a mobile laser Doppler velocimeter to track and monitor the transport and dispersion of aerial spray generated by an agricultural aircraft.

Sugars en route to the roots. Transport, metabolism and storage within plant roots and towards microorganisms of the rhizosphere.

PubMed

Hennion, Nils; Durand, Mickael; Vriet, Cécile; Doidy, Joan; Maurousset, Laurence; Lemoine, Rémi; Pourtau, Nathalie

2018-04-28

In plants, root is a typical sink organ that relies exclusively on the import of sugar from the aerial parts. Sucrose is delivered by the phloem to the most distant root tips and, en route to the tip, is used by the different root tissues for metabolism and storage. Besides, a certain portion of this carbon is exuded in the rhizosphere, supplied to beneficial microorganisms and diverted by parasitic microbes. The transport of sugars towards these numerous sinks either occurs symplastically through cell connections (plasmodesmata) or is apoplastically mediated through membrane transporters (MST, SUT/SUC and SWEET) that control monosaccharide and sucrose fluxes. Here, we review recent progresses on carbon partitioning within and outside roots, discussing membrane transporters involved in plant responses to biotic and abiotic factors. This article is protected by copyright. All rights reserved.

36 CFR 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

Code of Federal Regulations, 2014 CFR

2014-07-01

... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the aerial...

36 CFR 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

Code of Federal Regulations, 2012 CFR

2012-07-01

... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the aerial...

36 CFR 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

Code of Federal Regulations, 2011 CFR

2011-07-01

... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the aerial...

36 CFR 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

Code of Federal Regulations, 2010 CFR

2010-07-01

... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the aerial...

Two micro-scale protocols for the isolation of DNA from polysaccharide-rich plant tissue.

PubMed

Shepherd, Lara D; McLay, Todd G B

2011-03-01

The high polysaccharide content of some plant species hinders the successful isolation of their DNA. As an alternative to the macro-extraction methods previously published for polysaccharide-rich plants, we present two techniques (STE/CTAB and HEPES/CTAB), which are performed in microcentrifuge tubes. These protocols are suitable for small amounts of silica gel-preserved plant tissue such as are commonly available from endangered plants. The critical step to remove polysaccharides was performing initial washes in either STE (0.25 M sucrose, 0.03 M Tris, 0.05 M EDTA) or HEPES (2% β-mercaptoethanol, 0.2% PVP, 0.1 M HEPES, pH 8.0) buffer. Precipitating the DNA at room temperature with isopropanol also aided in decreasing polysaccharide co-precipitation. Of the two protocols we present the STE/CTAB method has the advantages of being more cost-effective and avoiding the use of the hazardous chemical β-mercaptoethanol.

The interaction of heavy metals and nutrients present in soil and native plants with arbuscular mycorrhizae on the riverside in the Matanza-Riachuelo River Basin (Argentina).

PubMed

Mendoza, Rodolfo E; García, Ileana V; de Cabo, Laura; Weigandt, Cristian F; Fabrizio de Iorio, Alicia

2015-02-01

This study assessed the contamination by heavy metals (Cr, Cu, Pb, Zn), and nutrients (N, P) in soils and native plants, and the effect of the concentration of those elements with the density of arbuscular-mycorrhizal (AM) spores in soil and colonization in roots from the riverside of the Matanza-Riachuelo River Basin (MRRB). The concentration of metals and nutrients in soils and plants (Eleocharis montana, Cyperus eragrostis, Hydrocotyle bonariensis) increased from the upper sites (8 km from headwaters) to the lower sites (6 km from the mouth of the Riachuelo River) of the basin. AM-colonization on the roots of H. bonariensis and spore density in soil decreased as the concentrations of metals in soil and plant tissues increased from the upper to lower sites of the basin within a consistent gradient of contamination associated with land use, soil disturbance, population, and chemicals discharged into the streams and rivers along the MRRB. The general trends for all metals in plant tissue were to have highest concentrations in roots, then in rhizomes and lowest in aerial biomass. The translocation (TF) and bioconcentration (BCF) factors decreased in plants which grow from the upper sites to the lower sites of the basin. The plants tolerated a wide range in type and quantity of contamination along the basin by concentrating more metals and nutrients in roots than in aboveground tissue. The AM spore density in soil and colonization in roots of H. bonariensis decreased with the increase of the degree of contamination (Dc) in soil. Copyright © 2014 Elsevier B.V. All rights reserved.

Monitoring and Assuring the Quality of Digital Aerial Data

NASA Technical Reports Server (NTRS)

Christopherson, Jon

2007-01-01

This viewgraph presentation explains the USGS plan for monitoring and assuring the quality of digital aerial data. The contents include: 1) History of USGS Aerial Imaging Involvement; 2) USGS Research and Results; 3) Outline of USGS Quality Assurance Plan; 4) Other areas of Interest; and 5) Summary

Aerial biomass and elemental changes in Atriplex canescens and A. acanthocarpa as affected by salinity and soil water availability

Treesearch

Ricardo Mata-Gonzalez; Ruben Melendez-Gonzalez; J. Jesus Martinez-Hernandez

2001-01-01

Atriplex canescens and A. acanthocarpa from the Chihuahuan Desert in Mexico were subjected to different salinity and irrigation treatments in a greenhouse study. Plants were grown in pots containing soil and irrigated with NaCl solutions of 0, 50, and 100 mM at 40 and 80 percent available soil water. Aerial biomass of A. canescens declined as NaCl treatments increased...

Detail design of empennage of an unmanned aerial vehicle

NASA Astrophysics Data System (ADS)

Sarker, Md. Samad; Panday, Shoyon; Rasel, Md; Salam, Md. Abdus; Faisal, Kh. Md.; Farabi, Tanzimul Hasan

2017-12-01

In order to maintain the operational continuity of air defense systems, unmanned autonomous or remotely controlled unmanned aerial vehicle (UAV) plays a great role as a target for the anti-aircraft weapons. The aerial vehicle must comply with the requirements of high speed, remotely controlled tracking and navigational aids, operational sustainability and sufficient loiter time. It can also be used for aerial reconnaissance, ground surveillance and other intelligence operations. This paper aims to develop a complete tail design of an unmanned aerial vehicle using Systems Engineering approach. The design fulfils the requirements of longitudinal and directional trim, stability and control provided by the horizontal and vertical tail. Tail control surfaces are designed to provide sufficient control of the aircraft in critical conditions. Design parameters obtained from wing design are utilized in the tail design process as required. Through chronological calculations and successive iterations, optimum values of 26 tail design parameters are determined.

Arctic Oil Spill Mapping and Response Using Unmanned Aerial Systems

NASA Astrophysics Data System (ADS)

Cunningham, K. W.

2011-12-01

The University of Alaska Fairbanks works extensively with unmanned aerial systems and various sensor payloads used in mapping. Recent projects with Royal Dutch Shell and British Petroleum have demonstrated that unmanned aerial systems, including fixed and rotary winged platforms, can provide quick response to oil spill mapping in a variety of flight conditions, including those not well suited for manned aerial systems. We describe this collaborative research between the University and oil companies exploring and developing oil resources in Alaska and the Arctic.

Measurements from an Aerial Vehicle: A New Tool for Planetary Exploration

NASA Technical Reports Server (NTRS)

Wright, Henry S.; Levine, Joel S.; Croom, Mark A.; Edwards, William C.; Qualls, Garry D.; Gasbarre, Joseph F.

2004-01-01

Aerial vehicles fill a unique planetary science measurement gap, that of regional-scale, near-surface observation, while providing a fresh perspective for potential discovery. Aerial vehicles used in planetary exploration bridge the scale and resolution measurement gaps between orbiters (global perspective with limited spatial resolution) and landers (local perspective with high spatial resolution) thus complementing and extending orbital and landed measurements. Planetary aerial vehicles can also survey scientifically interesting terrain that is inaccessible or hazardous to landed missions. The use of aerial assets for performing observations on Mars, Titan, or Venus will enable direct measurements and direct follow-ons to recent discoveries. Aerial vehicles can be used for remote sensing of the interior, surface and atmosphere of Mars, Venus and Titan. Types of aerial vehicles considered are airplane "heavier than air" and airships and balloons "lighter than air". Interdependencies between the science measurements, science goals and objectives, and platform implementation illustrate how the proper balance of science, engineering, and cost, can be achieved to allow for a successful mission. Classification of measurement types along with how those measurements resolve science questions and how these instruments are accommodated within the mission context are discussed.

Human health risk assessment of pharmaceuticals and personal care products in plant tissue due to biosolids and manure amendments, and wastewater irrigation.

PubMed

Prosser, R S; Sibley, P K

2015-02-01

Amending soil with biosolids or livestock manure provides essential nutrients in agriculture. Irrigation with wastewater allows for agriculture in regions where water resources are limited. However, biosolids, manure and wastewater have all been shown to contain pharmaceuticals and personal care products (PPCPs). Studies have shown that PPCPs can accumulate in the tissues of plants but the risk that accumulated residues may pose to humans via consumption of edible portions is not well documented. This study reviewed the literature for studies that reported residues of PPCPs in the edible tissue of plants grown in biosolids- or manure-amended soils or irrigated with wastewater. These residues were used to determine the estimated daily intake of PPCPs for an adult and toddler. Estimated daily intake values were compared to acceptable daily intakes to determine whether PPCPs in plant tissue pose a hazard to human health. For all three amendment practices, the majority of reported residues resulted in hazard quotients <0.1. Amendment with biosolids or manure resulted in hazard quotients ≥0.1 for carbamazepine, diphenhydramine, salbutamol, triclosan, and sulfamethazine. Irrigation with wastewater resulted in hazard quotients of ≥0.1 for ambrettolid, carbamazepine, diclofenac, flunixin, lamotrigine, metoprolol, naproxen, sildenafil and tonalide. [corrected]. Many of the residues that resulted in hazard quotients ≥0.1 were due to exposing plants to concentrations of PPCPs that would not be considered relevant based on concentrations reported in biosolids and manure or unrealistic methods of exposure, which lead to artificially elevated plant residues. Our assessment indicates that the majority of individual PPCPs in the edible tissue of plants due to biosolids or manure amendment or wastewater irrigation represent a de minimis risk to human health. Assuming additivity, the mixture of PPCPs could potentially present a hazard. Further work needs to be done to assess

Fuzzy C-Means Algorithm for Segmentation of Aerial Photography Data Obtained Using Unmanned Aerial Vehicle

NASA Astrophysics Data System (ADS)

Akinin, M. V.; Akinina, N. V.; Klochkov, A. Y.; Nikiforov, M. B.; Sokolova, A. V.

2015-05-01

The report reviewed the algorithm fuzzy c-means, performs image segmentation, give an estimate of the quality of his work on the criterion of Xie-Beni, contain the results of experimental studies of the algorithm in the context of solving the problem of drawing up detailed two-dimensional maps with the use of unmanned aerial vehicles. According to the results of the experiment concluded that the possibility of applying the algorithm in problems of decoding images obtained as a result of aerial photography. The considered algorithm can significantly break the original image into a plurality of segments (clusters) in a relatively short period of time, which is achieved by modification of the original k-means algorithm to work in a fuzzy task.

Herbicidal drift control: aerial spray equipment, formulations, and supervision.

Treesearch

H. Gratkowski

1974-01-01

Public concern over environmental pollution requires increasingly sophisticated procedures when herbicides are used in silviculture. Many specialized aerial application systems and spray additives have been developed to reduce drift of herbicidal sprays. This publication provides forest-land managers with a brief description of these aerial spray systems and additives...

Calculated Drag of an Aerial Refueling Assembly Through Airplane Performance Analysis

NASA Technical Reports Server (NTRS)

Vachon, Jake; Ray, Ronald; Calianno, Carl

2004-01-01

This viewgraph document reviews NASA Dryden's work on Aerial refueling, with specific interest in calculating the drag of the refueling system. The aerodynamic drag of an aerial refueling assembly was calculated during the Automated Aerial Refueling project at the NASA Dryden Flight Research Center. An F/A-18A airplane was specially instrumented to obtain accurate fuel flow measurements and to determine engine thrust

Phyto-dehydration of confined polluted sludge: impacts on C-storage and heavy metal immobilization in plant tissues

NASA Astrophysics Data System (ADS)

Liberati, Dario; Sconocchia, Paolo; Ricci, Anna; Gigliotti, Giovanni; Tacconi, Chiara; De Angelis, Paolo

2017-04-01

Transpiration of plants can be used to control or remove water in artificial basins containing polluted flooded sediments (phyto-dehydration), with the aim to reduce the risk of environment contamination due to water/sediment spillage. At the same time plants can reduce the risks associated to the pollutants, reducing their mobility by the adsorption in the rhizosphere, uptake and accumulation in tissues, and providing organiccompounds contributing to bind heavy metals. We tested, at pilot scale, a phytodeydration approach to be applied to a storage pond containing sludge with high zinc and copper concentrations (3200 and 1000 µg/Kg, respectively). The sludge derives from the biodigestion of pig slurries, and for most of the year is covered by a water layer due to rainfall. The phyto-dehydration approach was tested in a two years long mesocosm-scale experiment. Inside the mesocosms we maintained the same sludge/water stratification observed in the pond; the helophyte species Phragmites australis was planted over a floating frame inside half of the mesocosms. Mesocosms with P.australis and control mesocosms without plants, were monitored during the test to assess the water consumption, CO2 and CH4 gas exchanges and plant functioning. At the end of the second year we analysed the changes on the carbon pool of the sludge and the immobilization of heavy metals in the plant tissues. After two years, the total organic carbon content of the sludge has been reduced in the control mesocosms, while in the P. australis mesocosms remain close to the initial values. Zinc and copper immobilization in the plant tissues, was characterised by: a very low concentration of zinc (5 µg/kg ) in leaves, intermediates values in culms and rhizomes (49 and 30 µg/kg) and higher values in roots (222 and 114 µg/kg). In conclusion, in addition to the reduction of the sludge spillage risks, the phyto-dehydration approach based on P. australis reduced the carbon loss of the sludge, and

Changes of ammonia, urea contents and transaminase activity in the body during aerial exposure and ammonia loading in Chinese loach Paramisgurnus dabryanus.

PubMed

Zhang, Yun-Long; Zhang, Hai-Long; Wang, Ling-Yu; Gu, Bei-Yi; Fan, Qi-Xue

2017-04-01

The Paramisgurnus dabryanus was exposed to 30 mmol L -1 NH 4 Cl solution and air to assessing the change of body ammonia and urea contents and the activities of alanine aminotransferase (ALT) and aspartate transaminase (AST). After 48 h of ammonia exposure, ammonia concentration in the plasma, brain, liver and muscle were 3.3-fold, 5.6-fold, 3.5-fold and 4.2-fold, respectively, those of the control values. Plasma, brain, liver and muscle ammonia concentrations increased to 2.2-fold, 3.3-fold, 2.5-fold and 2.9-fold, respectively, those of control values in response to 48 h of aerial exposure. Within the given treatment (ammonia or aerial exposure), there was no change in plasma, brain and liver urea concentrations between exposure durations. The plasma ALT activity was significantly affected by exposure time during aerial exposure, while the liver ALT activity was not affected by ammonia or aerial exposure. Exposure to NH 4 Cl or air had no effect on either plasma or liver AST activity. Our results suggested that P. dabryanus could accumulate quite high level of internal ammonia because of the high ammonia tolerance in its cells and tissues, and NH 3 volatilization would be a possible ammonia detoxification strategy in P. dabryanus. Urea synthesis was not an effective mechanism to deal with environmental or internal ammonia problem. The significant increase of ALT activity in plasma during aerial exposure, indicating that alanine synthesis through certain amino acid catabolism may be subsistent in P. dabryanus.

Cellular Force Microscopy for in Vivo Measurements of Plant Tissue Mechanics1[W][OA

PubMed Central

Routier-Kierzkowska, Anne-Lise; Weber, Alain; Kochova, Petra; Felekis, Dimitris; Nelson, Bradley J.; Kuhlemeier, Cris; Smith, Richard S.

2012-01-01

Although growth and morphogenesis are controlled by genetics, physical shape change in plant tissue results from a balance between cell wall loosening and intracellular pressure. Despite recent work demonstrating a role for mechanical signals in morphogenesis, precise measurement of mechanical properties at the individual cell level remains a technical challenge. To address this challenge, we have developed cellular force microscopy (CFM), which combines the versatility of classical microindentation techniques with the high automation and resolution approaching that of atomic force microscopy. CFM’s large range of forces provides the possibility to map the apparent stiffness of both plasmolyzed and turgid tissue as well as to perform micropuncture of cells using very high stresses. CFM experiments reveal that, within a tissue, local stiffness measurements can vary with the level of turgor pressure in an unexpected way. Altogether, our results highlight the importance of detailed physically based simulations for the interpretation of microindentation results. CFM’s ability to be used both to assess and manipulate tissue mechanics makes it a method of choice to unravel the feedbacks between mechanics, genetics, and morphogenesis. PMID:22353572

Estimating tree heights from shadows on vertical aerial photographs

Treesearch

Earl J. Rogers

1947-01-01

Aerial photographs are now being applied more and more to practical forestry - especially to forest survey. Many forest characteristics can be recognized on aerial photographs in greater detail than is possible through ground methods alone. The basic need is for tools and methods for interpreting the detail in quantitative terms.

Toxic hazards in aerial application.

DOT National Transportation Integrated Search

1962-04-01

An analysis of the hazards accompanying the aerial application of toxic pest-control chemicals are presented. The nature of teh chemicals, teh symptoms of toxicity, recommended treatment, and suggestions for safe-handling, are discussed

Applicability of New Approaches of Sensor Orientation to Micro Aerial Vehicles

NASA Astrophysics Data System (ADS)

Rehak, M.; Skaloud, J.

2016-06-01

This study highlights the benefits of precise aerial position and attitude control in the context of mapping with Micro Aerial Vehicles (MAVs). Accurate mapping with MAVs is gaining importance in applications such as corridor mapping, road and pipeline inspections or mapping of large areas with homogeneous surface structure, e.g. forests or agricultural fields. There, accurate aerial control plays a major role in successful terrain reconstruction and artifact-free ortophoto generation. The presented experiments focus on new approaches of aerial control. We confirm practically that the relative aerial position and attitude control can improve accuracy in difficult mapping scenarios. Indeed, the relative orientation method represents an attractive alternative in the context of MAVs for two reasons. First, the procedure is somewhat simplified, e.g. the angular misalignment, so called boresight, between the camera and the inertial measurement unit (IMU) does not have to be determined and, second, the effect of possible systematic errors in satellite positioning (e.g. due to multipath and/or incorrect recovery of differential carrier-phase ambiguities) is mitigated. First, we present a typical mapping project over an agricultural field and second, we perform a corridor road mapping. We evaluate the proposed methods in scenarios with and without automated image observations. We investigate a recently proposed concept where adjustment is performed using image observations limited to ground control and check points, so called fast aerial triangulation (Fast AT). In this context we show that accurate aerial control (absolute or relative) together with a few image observations can deliver accurate results comparable to classical aerial triangulation with thousands of image measurements. This procedure in turns reduces the demands on processing time and the requirements on the existence of surface texture. Finally, we compare the above mentioned procedures with direct sensor

ARM Unmanned Aerial Systems Implementation Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmid, Beat; Ivey, Mark

Recent advances in Unmanned Aerial Systems (UAS) coupled with changes in the regulatory environment for operations of UAS in the National Airspace increase the potential value of UAS to the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility. UAS include unmanned aerial vehicles (UAV) and tethered balloon systems (TBS). The roles UAVs and TBSs could play within the ARM Facility, particularly science questions they could help address, have been discussed in several workshops, reports, and vision documents, including: This document describes the implementation of a robust and vigorous program for use of UAV and TBS formore » the science missions ARM supports.« less

Spectral anomaly methods for aerial detection using KUT nuisance rejection

NASA Astrophysics Data System (ADS)

Detwiler, R. S.; Pfund, D. M.; Myjak, M. J.; Kulisek, J. A.; Seifert, C. E.

2015-06-01

This work discusses the application and optimization of a spectral anomaly method for the real-time detection of gamma radiation sources from an aerial helicopter platform. Aerial detection presents several key challenges over ground-based detection. For one, larger and more rapid background fluctuations are typical due to higher speeds, larger field of view, and geographically induced background changes. As well, the possible large altitude or stand-off distance variations cause significant steps in background count rate as well as spectral changes due to increased gamma-ray scatter with detection at higher altitudes. The work here details the adaptation and optimization of the PNNL-developed algorithm Nuisance-Rejecting Spectral Comparison Ratios for Anomaly Detection (NSCRAD), a spectral anomaly method previously developed for ground-based applications, for an aerial platform. The algorithm has been optimized for two multi-detector systems; a NaI(Tl)-detector-based system and a CsI detector array. The optimization here details the adaptation of the spectral windows for a particular set of target sources to aerial detection and the tailoring for the specific detectors. As well, the methodology and results for background rejection methods optimized for the aerial gamma-ray detection using Potassium, Uranium and Thorium (KUT) nuisance rejection are shown. Results indicate that use of a realistic KUT nuisance rejection may eliminate metric rises due to background magnitude and spectral steps encountered in aerial detection due to altitude changes and geographically induced steps such as at land-water interfaces.

Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis

USDA-ARS?s Scientific Manuscript database

As the outermost layer on aerial tissues of the primary plant body, the cuticle plays important roles in plant development and physiology. The major components of the cuticle are cutin and cuticular wax, both of which are composed primarily of fatty acid derivatives synthesized in the epidermal cell...

United States Air Force in Southeast Asia 1965-1973. Aces and Aerial Victories

DTIC Science & Technology

1976-01-01

UNITED STATES IN SOUTHEAST ACES and AERIAL VICTORIES The United States Air Force in SoutheastAsia 1965*19?3 by R Frank Fuirefl William H...TYPE 3. DATES COVERED 00-00-1976 to 00-00-1976 4. TITLE AND SUBTITLE United States Air Force in Southeast Asia 1965-1973. Aces and Aerial... aces and aerial victories, 1965-1973. Includes index and glossary. 1. Vietnamese Conflict, 1961-1975-Aerial operations, American. 2. Viet- namese

AERIAL OF SHUTTLE LANDING FACILITY [SLF] POURING CONCRETE ON RUNWAY

NASA Technical Reports Server (NTRS)

1975-01-01

AERIAL OF SHUTTLE LANDING FACILITY [SLF] POURING CONCRETE ON RUNWAY KSC-375C-10036.32 108-KSC-375C-10036.32, P-21425, ARCHIVE-04501 Aerial oblique of Shuttle Landing Facility. Pouring concrete on runway. Direction North - Altitude 100'.

AERIAL OF SHUTTLE LANDING FACILITY [SLF] SAFING AND PARKING AREA

NASA Technical Reports Server (NTRS)

1975-01-01

AERIAL OF SHUTTLE LANDING FACILITY [SLF] SAFING AND PARKING AREA KSC-375C-0654.18 108-KSC-375C-654.18, P-18812, ARCHIVE-04493 Aerial oblique - safing and parking area, Shuttle Orbiter Landing Facility. Altitude 800' direction- west.

Assessment of forest plantations from low altitude aerial photography. [North Carolina coastal plains

NASA Technical Reports Server (NTRS)

Nelson, H. A.

1977-01-01

Vertical color, and color-infrared, aerial photography obtained from altitudes between 183 m and 915 m provide a cost effective method of determining tree survival and height growth in pine plantations on the North Carolina Coastal Plain. All interpretations were performed by professional forestry personnel from the original 70 mm color transparencies. Prompt assessment of tree survival is necessary if failed spots are to be successfully replanted. Counts of living trees made after the third growing season, and sometimes only two growing seasons after planting, are accurate enough to permit planning of replanting operations without extensive ground surveys.

Role of different salt marsh plants on metal retention in an urban estuary (Lima estuary, NW Portugal)

NASA Astrophysics Data System (ADS)

Almeida, C. M. R.; Mucha, Ana P.; Teresa Vasconcelos, M.

2011-01-01

The aim of the present work was to understand the role different salt marsh plants on metal distribution and retention in the Lima River estuary (NW Portugal), which to our knowledge have not been ascertained in this area yet. The knowledge of these differences is an important requirement for the development of appropriate management strategies, and is poorly described for Eurosiberian estuaries, like the one selected. In addition it is important to understand the difference among introduced and native salt marsh plants. In this work, metal levels (Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) were surveyed (by atomic absorption spectrometry) in sediments from sites vegetated with Juncus maritimus, Spartina patens, Phragmites australis and Triglochin striata (rhizo-sediments), in non-vegetated sediments and in the different tissues of the plants (roots, rhizomes and aerial shoots). In general, rhizo-sediments had higher metal concentrations than non-vegetated sediments, a feature that seems common to sediments colonized by salt marsh plants of different estuarine areas. All plants concentrated metals, at least Cd, Cu and Zn (and Pb for T. striata) in their belowground structures ([ M] belowground tissues/[ M] non-vegetated sediment > 1). However, when considered per unit of salt marsh area, the different selected plants played a different role on sediment metal distribution and retention. Triglochin striata retained a significant metal burden in it belowground structures (root plus rhizomes) acting like a possible phyto-stabilizer, whereas P. australis had an higher metal burden in aboveground tissues acting as a possible phyto-extractor. As for J. maritimus and S. patens, metal burden distribution between above and belowground structures depended on the metal, with J. maritimus retaining, for instance, much more Cd and Cu in the aboveground than in the belowground structures. Therefore, the presence of invasive and exotic plants in some areas of the salt marsh may

77 FR 36250 - Information Collection Request; Request for Aerial Photography

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-18

... the responsibility for conducting and coordinating the FSA's aerial photography, remote sensing... FSA Aerial Photography Field Office (APFO) uses the information from this form to collect the customer... respond, including through the use of appropriate automated, electronic, mechanical, or other...

Short Communication An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues.

PubMed

Oliveira, R R; Viana, A J C; Reátegui, A C E; Vincentz, M G A

2015-12-29

Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA cytosine methylation, which is an epigenetic mark. Correlation of gene expression with DNA sequence or epigenetic mark polymorphism is often desirable; for this, a robust protocol to isolate high-quality RNA and DNA simultaneously from the same sample is required. Although commercial kits and protocols are available, they are mainly optimized for animal tissues and, in general, restricted to RNA or DNA extraction, not both. In the present study, we describe an efficient and accessible method to extract both RNA and DNA simultaneously from the same sample of various plant tissues, using small amounts of starting material. The protocol was efficient in the extraction of high-quality nucleic acids from several Arabidopsis thaliana tissues (e.g., leaf, inflorescence stem, flower, fruit, cotyledon, seedlings, root, and embryo) and from other tissues of non-model plants, such as Avicennia schaueriana (Acanthaceae), Theobroma cacao (Malvaceae), Paspalum notatum (Poaceae), and Sorghum bicolor (Poaceae). The obtained nucleic acids were used as templates for downstream analyses, such as mRNA sequencing, quantitative real time-polymerase chain reaction, bisulfite treatment, and others; the results were comparable to those obtained with commercial kits. We believe that this protocol could be applied to a broad range of plant species, help avoid technical and sampling biases, and facilitate several RNA- and DNA-dependent analyses.

36 CFR § 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

Code of Federal Regulations, 2013 CFR

2013-07-01

... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the aerial...

The ABCs of New Zealand Sign Language: Aerial Spelling.

ERIC Educational Resources Information Center

Forman, Wayne

2003-01-01

Aerial spelling is the term given for the way many people with deafness in New Zealand (NZ) manually represent letters of the alphabet. This article examines the nature and role of aerial spelling in New Zealand Sign Language, particularly that form used by older members of the NZ deaf community. (Contains references.) (Author/CR)

Application possibilities of aerial and terrain data evaluation in particulate pollution effects

NASA Astrophysics Data System (ADS)

Kozma-Bognar, V.; Berke, J.; Martin, G.

2012-04-01

Recently, remote sensing has become a widely used technology in order to acquire information about our environment. Data collected using remote sensing technology indispensible criteria to recognise and monitor environmental problems caused by contamination from various human activities. According to great technological change and development in the previous decade high spectral and geometric resolution sensors are more often used. The higher resolution technology allows getting more accurate and reliable results in the research processes of the environmental pollution impacts. At University of Pannonia, Georgikon Faculty (Hungary) plant-soil-atmosphere system analyses are carried out for detecting the potential harmful effects of heavy metal pollution originated from vehicle industry. Related to this research at the Department of Meteorology and Water Management, black carbon and cadmium pollution effects are being analysed on maize crops. Testing area is situated at Agro-meteorological Research Station in Keszthely, where the first time in 2011 aerial imaging technology was used in parallel with field analyses. The experiment aims to analyses correlation of the field data with aerial data. During aerial photography were taken in different spectral bands (Visible, Near Infrared, Far Infrared). High intensity, spectral and spatial resolution data was an important part of the multitemporal imagine sensing and evaluating technology, therefore original technical solutions were applied. These resolutions served accurate plot-level evaluation. Fractal structure and intensity measurement evaluation methods were applied to examine black carbon and cadmium polluted and control maize canopy after data pre-processing. Research also focused on the examination of potential negative or positive effects of irrigation so that differences between irrigated and non-irrigated maize was investigated. For the period of growing season of 2011 time-series analyses were carried out in

Research of aerial imaging spectrometer data acquisition technology based on USB 3.0

NASA Astrophysics Data System (ADS)

Huang, Junze; Wang, Yueming; He, Daogang; Yu, Yanan

2016-11-01

With the emergence of UAV (unmanned aerial vehicle) platform for aerial imaging spectrometer, research of aerial imaging spectrometer DAS(data acquisition system) faces new challenges. Due to the limitation of platform and other factors, the aerial imaging spectrometer DAS requires small-light, low-cost and universal. Traditional aerial imaging spectrometer DAS system is expensive, bulky, non-universal and unsupported plug-and-play based on PCIe. So that has been unable to meet promotion and application of the aerial imaging spectrometer. In order to solve these problems, the new data acquisition scheme bases on USB3.0 interface.USB3.0 can provide guarantee of small-light, low-cost and universal relying on the forward-looking technology advantage. USB3.0 transmission theory is up to 5Gbps.And the GPIF programming interface achieves 3.2Gbps of the effective theoretical data bandwidth.USB3.0 can fully meet the needs of the aerial imaging spectrometer data transmission rate. The scheme uses the slave FIFO asynchronous data transmission mode between FPGA and USB3014 interface chip. Firstly system collects spectral data from TLK2711 of high-speed serial interface chip. Then FPGA receives data in DDR2 cache after ping-pong data processing. Finally USB3014 interface chip transmits data via automatic-dma approach and uploads to PC by USB3.0 cable. During the manufacture of aerial imaging spectrometer, the DAS can achieve image acquisition, transmission, storage and display. All functions can provide the necessary test detection for aerial imaging spectrometer. The test shows that system performs stable and no data lose. Average transmission speed and storage speed of writing SSD can stabilize at 1.28Gbps. Consequently ,this data acquisition system can meet application requirements for aerial imaging spectrometer.

The sky is the limit? 20 years of small-format aerial photography taken from UAS for monitoring geomorphological processes

NASA Astrophysics Data System (ADS)

Marzolff, Irene

2014-05-01

One hundred years after the first publication on aerial photography taken from unmanned aerial platforms (Arthur Batut 1890), small-format aerial photography (SFAP) became a distinct niche within remote sensing during the 1990s. Geographers, plant biologists, archaeologists and other researchers with geospatial interests re-discovered the usefulness of unmanned platforms for taking high-resolution, low-altitude photographs that could then be digitized and analysed with geographical information systems, (softcopy) photogrammetry and image processing techniques originally developed for digital satellite imagery. Even before the ubiquity of digital consumer-grade cameras and 3D analysis software accessible to the photogrammetric layperson, do-it-yourself remote sensing using kites, blimps, drones and micro air vehicles literally enabled the questing researcher to get their own pictures of the world. As a flexible, cost-effective method, SFAP offered images with high spatial and temporal resolutions that could be ideally adapted to the scales of landscapes, forms and distribution patterns to be monitored. During the last five years, this development has been significantly accelerated by the rapid technological advancements of GPS navigation, autopiloting and revolutionary softcopy-photogrammetry techniques. State-of-the-art unmanned aerial systems (UAS) now allow automatic flight planning, autopilot-controlled aerial surveys, ground control-free direct georeferencing and DEM plus orthophoto generation with centimeter accuracy, all within the space of one day. The ease of use of current UAS and processing software for the generation of high-resolution topographic datasets and spectacular visualizations is tempting and has spurred the number of publications on these issues - but which advancements in our knowledge and understanding of geomorphological processes have we seen and can we expect in the future? This presentation traces the development of the last two decades

Ontogeny of aerial righting and wing flapping in juvenile birds.

PubMed

Evangelista, Dennis; Cam, Sharlene; Huynh, Tony; Krivitskiy, Igor; Dudley, Robert

2014-08-01

Mechanisms of aerial righting in juvenile chukar partridge (Alectoris chukar) were studied from hatching to 14 days-post-hatching (dph). Asymmetric movements of the wings were used from 1 to 8 dph to effect progressively more successful righting behaviour via body roll. Following 8 dph, wing motions transitioned to bilaterally symmetric flapping that yielded aerial righting via nose-down pitch, along with substantial increases in vertical force production during descent. Ontogenetically, the use of such wing motions to effect aerial righting precedes both symmetric flapping and a previously documented behaviour in chukar (i.e. wing-assisted incline running) hypothesized to be relevant to incipient flight evolution in birds. These findings highlight the importance of asymmetric wing activation and controlled aerial manoeuvres during bird development and are potentially relevant to understanding the origins of avian flight. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Aerial Views of KSC

NASA Image and Video Library

2003-07-23

This aerial view, looking northwest, shows the newly opened Space Commerce Way that winds from S.R 3 on the right to its exit on S.R. 405 (near the top) in the background. The road is the new public access from Merritt Island to the KSC Visitor Complex (seen at top).

Sucrose-induced anthocyanin accumulation in vegetative tissue of Petunia plants requires anthocyanin regulatory transcription factors.

PubMed

Ai, Trinh Ngoc; Naing, Aung Htay; Arun, Muthukrishnan; Lim, Sun-Hyung; Kim, Chang Kil

2016-11-01

The effects of three different sucrose concentrations on plant growth and anthocyanin accumulation were examined in non-transgenic (NT) and transgenic (T 2 ) specimens of the Petunia hybrida cultivar 'Mirage rose' that carried the anthocyanin regulatory transcription factors B-Peru+mPAP1 or RsMYB1. Anthocyanin accumulation was not observed in NT plants in any treatments, whereas a range of anthocyanin accumulation was observed in transgenic plants. The anthocyanin content detected in transgenic plants expressing the anthocyanin regulatory transcription factors (B-Peru+mPAP1 or RsMYB1) was higher than that in NT plants. In addition, increasing sucrose concentration strongly enhanced anthocyanin content as shown by quantitative real-time polymerase chain reaction (qRT-PCR) analysis, wherein increased concentrations of sucrose enhanced transcript levels of the transcription factors that are responsible for the induction of biosynthetic genes involved in anthocyanin synthesis; this pattern was not observed in NT plants. In addition, sucrose affected plant growth, although the effects were different between NT and transgenic plants. Taken together, the application of sucrose could enhance anthocyanin production in vegetative tissue of transgenic Petunia carrying anthocyanin regulatory transcription factors, and this study provides insights about interactive effects of sucrose and transcription factors in anthocyanin biosynthesis in the transgenic plant. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Xanthan gum: an economical substitute for agar in plant tissue culture media.

PubMed

Jain, R; Babbar, S B

2006-03-01

Xanthan gum, a microbial desiccation-resistant polysaccharide prepared commercially by aerobic submerged fermentation from Xanthomonas campestris, has been successfully used as a solidifying agent for plant tissue culture media. Its suitability as a substitute to agar was demonstrated for in vitro seed germination, caulogenesis and rhizogenesis of Albizzia lebbeck, androgenesis in anther cultures of Datura innoxia, and somatic embryogenesis in callus cultures of Calliandra tweedii. Culture media used for eliciting these morphogenic responses were gelled with either 1% xanthan gum or 0.9% agar. Xanthan gum, like agar, supported all these responses.

ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology

PubMed Central

De Col, Valentina; Fuchs, Philippe; Nietzel, Thomas; Elsässer, Marlene; Voon, Chia Pao; Candeo, Alessia; Seeliger, Ingo; Fricker, Mark D; Grefen, Christopher; Møller, Ian Max; Bassi, Andrea; Lim, Boon Leong; Zancani, Marco; Meyer, Andreas J; Costa, Alex; Wagner, Stephan; Schwarzländer, Markus

2017-01-01

Growth and development of plants is ultimately driven by light energy captured through photosynthesis. ATP acts as universal cellular energy cofactor fuelling all life processes, including gene expression, metabolism, and transport. Despite a mechanistic understanding of ATP biochemistry, ATP dynamics in the living plant have been largely elusive. Here, we establish MgATP2- measurement in living plants using the fluorescent protein biosensor ATeam1.03-nD/nA. We generate Arabidopsis sensor lines and investigate the sensor in vitro under conditions appropriate for the plant cytosol. We establish an assay for ATP fluxes in isolated mitochondria, and demonstrate that the sensor responds rapidly and reliably to MgATP2- changes in planta. A MgATP2- map of the Arabidopsis seedling highlights different MgATP2- concentrations between tissues and within individual cell types, such as root hairs. Progression of hypoxia reveals substantial plasticity of ATP homeostasis in seedlings, demonstrating that ATP dynamics can be monitored in the living plant. DOI: http://dx.doi.org/10.7554/eLife.26770.001 PMID:28716182

Aerial vehicles collision avoidance using monocular vision

NASA Astrophysics Data System (ADS)

Balashov, Oleg; Muraviev, Vadim; Strotov, Valery

2016-10-01

In this paper image-based collision avoidance algorithm that provides detection of nearby aircraft and distance estimation is presented. The approach requires a vision system with a single moving camera and additional information about carrier's speed and orientation from onboard sensors. The main idea is to create a multi-step approach based on a preliminary detection, regions of interest (ROI) selection, contour segmentation, object matching and localization. The proposed algorithm is able to detect small targets but unlike many other approaches is designed to work with large-scale objects as well. To localize aerial vehicle position the system of equations relating object coordinates in space and observed image is solved. The system solution gives the current position and speed of the detected object in space. Using this information distance and time to collision can be estimated. Experimental research on real video sequences and modeled data is performed. Video database contained different types of aerial vehicles: aircrafts, helicopters, and UAVs. The presented algorithm is able to detect aerial vehicles from several kilometers under regular daylight conditions.

Growth of plant tissue cultures in simulated lunar soil: Implications for a lunar base CELSS (Controlled Ecological Life Support System)

NASA Technical Reports Server (NTRS)

Venketeswaran, S.

1988-01-01

Experiments were carried out on plant tissue cultures, seed germination, seedling development and plants grown on Simulated Lunar Soil to evaluate the potential of future development of lunar based agriculture. The studies done to determine the effect of the placement of SLS on tissue cultures showed no adverse effect of SLS on tissue cultures. Although statistically insignificant, SLS in suspension showed a comparatively higher growth rate. Observations indicate the SLS, itself cannot support calli growth but was able to show a positive effect on growth rate of calli when supplemented with MS salts. This positive effect related to nutritive value of the SLS was found to have improved at high pH levels, than at the recommended low pH levels for standard media. Results from seed germination indicated that there is neither inhibitory, toxicity nor stimulatory effect of SLS, even though SLS contains high amounts of aluminum compounds compared to earth soil. Analysis of seeding development and growth data showed significant reduction in growth rate indicating that, SLS was a poor growth medium for plant life. This was confirmed by the studies done with embryos and direct plant growth on SLS. Further observations attributed this poor quality of SLS is due to it's lack of essential mineral elements needed for plant growth. By changing the pH of the soil, to more basic conditions, the quality of SLS for plant growth could be improved up to a significant level. Also it was found that the quality of SLS could be improved by almost twice, by external supply of major mineral elements, directly to SLS.

Utilization of Local Law Enforcement Aerial Resources in Consequence Management (CM) Response

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wasiolek, Piotr T.; Malchow, Russell L.

2013-03-12

During the past decade the U.S. Department of Homeland Security (DHS) was instrumental in enhancing the nation’s ability to detect and prevent a radiological or nuclear attack in the highest risk cities. Under the DHS Securing the Cities initiative, nearly 13,000 personnel in the New York City region have been trained in preventive radiological and nuclear detection operations, and nearly 8,500 pieces of radiological detection equipment have been funded. As part of the preventive radiological/nuclear detection (PRND) mission, several cities have received funding to purchase commercial aerial radiation detection systems. In 2008, the U.S. Department of Energy, National Nuclear Securitymore » Administration Aerial Measuring System (AMS) program started providing Mobile Aerial Radiological Surveillance (MARS) training to such assets, resulting in over 150 HAZMAT teams’ officers and pilots from 10 law enforcement organizations and fire departments being trained in the aerial radiation detection. From the beginning, the MARS training course covered both the PRND and consequence management (CM) missions. Even if the law enforcement main focus is PRND, their aerial assets can be utilized in the collection of initial radiation data for post-event radiological CM response. Based on over 50 years of AMS operational experience and information collected during MARS training, this presentation will focus on the concepts of CM response using aerial assets as well as utilizing law enforcement/fire department aerial assets in CM. Also discussed will be the need for establishing closer relationships between local jurisdictions’ aerial radiation detection capabilities and state and local radiation control program directors, radiological health department managers, etc. During radiological events these individuals may become primary experts/advisers to Incident Commanders for radiological emergency response, especially in the early stages of a response. The knowledge of the

Inertial instrument system for aerial surveying

USGS Publications Warehouse

Brown, R.H.; Chapman, W.H.; Hanna, W.F.; Mongan, C.E.; Hursh, J.W.

1985-01-01

An inertial guidance system for aerial surveying has been developed under contract to the U.S. Geological Survey. This prototype system, known as the aerial profiling of terrain (APT) system, is designed to determine continuously the positions of points along an aircraft flight path, or the underlying terrain profile, to an accuracy of + or - 0.5 ft (15 cm) vertically and + or - 2 ft (61 cm) horizontally. The system 's objective thus is to accomplish, from a fixed-wing aircraft, what would traditionally be accomplished from ground-based topographic surveys combined with aerial photography and photogrammetry. The two-part strategy for measuring the terrain profile entails: (1) use of an inertial navigator for continuous determination of the three-coordinate position of the aircraft, and (2) use of an eye-safe pulsed laser profiler for continuous measurement of the vertical distance from aircraft to land surface, so that the desired terrain profile can then be directly computed. The APT system, installed in a DeHavilland Twin Otter aircraft, is typically flown at a speed of 115 mph (105 knots) at an altitude of 2,000 ft (610 m) above the terrain. Performance-evaluation flights have shown that the vertical and horizontal accuracy specifications are met. (USGS)

A facile means for the identification of indolic compounds from plant tissues.

PubMed

Yu, Peng; Hegeman, Adrian D; Cohen, Jerry D

2014-09-01

The bulk of indole-3-acetic acid (IAA) in plants is found in the form of conjugated molecules, yet past research on identifying these compounds has largely relied on methods that were both laborious and inefficient. Using recent advances in analytical instrumentation, we have developed a simple yet powerful liquid chromatography-mass spectrometry (LC-MS)-based method for the facile characterization of the small IAA conjugate profile of plants. The method uses the well-known quinolinium ion (m/z 130.0651) generated in MS processes as a signature with high mass accuracy that can be used to screen plant extracts for indolic compounds, including IAA conjugates. We reinvestigated Glycine max (soybean) for its indoles and found indole-3-acetyl-trytophan (IA-Trp) in addition to the already known indole-3-acetyl-aspartic acid (IA-Asp) and indole-3-acetyl-glutamic acid (IA-Glu) conjugates. Surprisingly, several organic acid conjugates of tryptophan were also discovered, many of which have not been reported in planta before. These compounds may have important physiological roles in tryptophan metabolism, which in turn can affect human nutrition. We also demonstrated the general applicability of this method by identifying indolic compounds in different plant tissues of diverse phylogenetic origins. It involves minimal sample preparation but can work in conjunction with sample enrichment techniques. This method enables quick screening of IAA conjugates in both previously characterized as well as uncharacterized species, and facilitates the identification of indolic compounds in general. © 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.

PLANT INCORPORATED PROTECTANT CROP MONITORING USING REMOTE SENSING

EPA Science Inventory

The extent of past and anticipated plantings of transgenic corn in the United States requires a new approach to monitor this important crop for the development of pest resistance. Remote sensing by aerial and/or satellite images may provide a method of identifying transgenic pest...

Aerial shows Stennis test stands

NASA Image and Video Library

2004-04-16

An aerial photo shows the B-1/B-2 Test Stand (foreground), A-2 Test Stand (middle) and A-1 Test Stand (back). The historic stands have been used to test engines used on every manned Apollo and space shuttle mission.

The remote characterization of vegetation using Unmanned Aerial Vehicle photography

USDA-ARS?s Scientific Manuscript database

Unmanned Aerial Vehicles (UAVs) can fly in place of piloted aircraft to gather remote sensing information on vegetation characteristics. The type of sensors flown depends on the instrument payload capacity available, so that, depending on the specific UAV, it is possible to obtain video, aerial phot...

Effect of moderate high temperature on the vegetative growth and potassium allocation in olive plants.

PubMed

Benlloch-González, María; Quintero, José Manuel; Suárez, María Paz; Sánchez-Lucas, Rosa; Fernández-Escobar, Ricardo; Benlloch, Manuel

2016-12-01

There is little information about the prolonged effect of a moderately high temperature on the growth of olive (Olea europaea L.). It has been suggested that when the temperature of the air rises above 35°C the shoot growth of olive is inhibited while there is any reference on how growth is affected when the soil is warmed. In order to examine these effects, mist-cuttings and young plants generated from seeds were grown under moderate high temperature (37°C) for 64 and 42days respectively. In our study, plant dry matter accumulation was reduced when the temperature of both the air and the root medium was moderately high. However, when the temperature of the root medium was 25°C, the inhibitory effect of air high temperature on plant growth was not observed. The exposure of both the aerial part and the root to moderate high temperature also reduced the accumulation of K + in the stem and the root, the water use efficiency and leaf relative water content. However, when only the aerial part was exposed to moderate high temperature, the accumulation of K + in the stem, the water use efficiency and leaf relative water content were not modified. The results from this study suggest that the olive is very efficient in regulating the water and potassium transport through the plant when only the atmosphere surrounding the aerial part is warmed up. However, an increase in the soil temperature decrease root K + uptake and its transport to the aerial parts resulting in a reduction in shoot water status and growth. Copyright © 2016 Elsevier GmbH. All rights reserved.

Aerial sampling using drones for measuring trace gases

NASA Astrophysics Data System (ADS)

Chang, Chih-Chung; Wang, Jia-Lin; Chang, Chih-Yuan; Lin, Ming-Ren; Ou-Yang, Chang-Feng; Pan, Xiang-Xu; Lin, Neng-Huei

2017-04-01

Aerial and ground-level samples were simultaneously collected at the northern tip of Taiwan, Cape Fuguei, which is commonly served as a receptor site to receive air parcels from East Asia, Asian continent, the northwest Pacific Ocean and the island of Taiwan itself. Both aerial and surface samples were analyzed for 106 volatile organic compounds (VOCs) and divided into three categories as follows: 1. the total concentrations of 106 VOC (T-VOC) at 300-m height that are lower than the T-VOC level at the surface (Category A), 2. T-VOC concentrations at 300-m that are higher than those at the surface (Category B), and 3. comparable concentrations (Category C). Ten VOCs were exploited as tracers for a variety of emission sources linking to possible sources and transport routes of airborne pollutants. The first two categories A and B showed significant differences in the observed composition and concentrations of tracers between aerial and ground-level samples, implying that the pollutants at different heights may have resulted from different sources and poor air mixing, despite only a 300-m difference in vertical height. In contrast, Category C showed good vertical mixing, as indicated by the comparable concentrations between the aerial and surface measurements. Since the three categories occurred in specific meteorological conditions (between, prior to, and after cold fronts), respectively, it suggests that varied prevailing meteorology controlled the distribution and transport of airborne pollutants. Unlike sampling commonly performed at the surface, this study uses aerial sampling to demonstrate that layered structures under different meteorological conditions. Sampling aloft in lower boundary layer avoids samples being over-influenced by the close-by surface sources such as traffic to reveal signatures of a broader region.

Rapid in situ detection of alkaloids in plant tissue under ambient conditions using desorption electrospray ionization.

PubMed

Talaty, Nari; Takáts, Zoltán; Cooks, R Graham

2005-12-01

Desorption electrospray ionization (DESI) mass spectrometry is applied to the in situ detection of alkaloids in the tissue of poison hemlock (Conium maculatum), jimsonweed (Datura stramonium) and deadly nightshade (Atropa belladonna). The experiment is carried out by electrospraying micro-droplets of solvent onto native or freshly-cut plant tissue surfaces. No sample preparation is required and the mass spectra are recorded under ambient conditions, in times of a few seconds. The impact of the sprayed droplets on the surface produces gaseous ions from organic compounds originally present in the plant tissue. The effects of operating parameters, including the electrospray high voltage, heated capillary temperature, the solvent infusion rate and the carrier gas pressure on analytical performance are evaluated and optimized. Different types of plant material are analyzed including seeds, stems, leaves, roots and flowers. All the previously reported alkaloids have been detected in C. maculatum, while fifteen out of nineteen known alkaloids for D. stramonium and the principal alkaloids of A. belladonna were also identified. All identifications were confirmed by tandem mass spectrometry. Results obtained show similar mass spectra, number of alkaloids, and signal intensities to those obtained when extraction and separation processes are performed prior to mass spectrometric analysis. Evidence is provided that DESI ionization occurs by both a gas-phase ionization process and by a droplet pick-up mechanism. Quantitative precision of DESI is compared with conventional electrospray ionization mass spectrometry (after sample workup) and the RSD values for the same set of 25 dicotyledonous C. maculatum seeds (one half of each seed analyzed by ESI and the other by DESI) are 9.8% and 5.2%, respectively.

A global analysis of parenchyma tissue fractions in secondary xylem of seed plants.

PubMed

Morris, Hugh; Plavcová, Lenka; Cvecko, Patrick; Fichtler, Esther; Gillingham, Mark A F; Martínez-Cabrera, Hugo I; McGlinn, Daniel J; Wheeler, Elisabeth; Zheng, Jingming; Ziemińska, Kasia; Jansen, Steven

2016-03-01

Parenchyma is an important tissue in secondary xylem of seed plants, with functions ranging from storage to defence and with effects on the physical and mechanical properties of wood. Currently, we lack a large-scale quantitative analysis of ray parenchyma (RP) and axial parenchyma (AP) tissue fractions. Here, we use data from the literature on AP and RP fractions to investigate the potential relationships of climate and growth form with total ray and axial parenchyma fractions (RAP). We found a 29-fold variation in RAP fraction, which was more strongly related to temperature than with precipitation. Stem succulents had the highest RAP values (mean ± SD: 70.2 ± 22.0%), followed by lianas (50.1 ± 16.3%), angiosperm trees and shrubs (26.3 ± 12.4%), and conifers (7.6 ± 2.6%). Differences in RAP fraction between temperate and tropical angiosperm trees (21.1 ± 7.9% vs 36.2 ± 13.4%, respectively) are due to differences in the AP fraction, which is typically three times higher in tropical than in temperate trees, but not in RP fraction. Our results illustrate that both temperature and growth form are important drivers of RAP fractions. These findings should help pave the way to better understand the various functions of RAP in plants. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Robotics and dynamic image analysis for studies of gene expression in plant tissues.

PubMed

Hernandez-Garcia, Carlos M; Chiera, Joseph M; Finer, John J

2010-05-05

Gene expression in plant tissues is typically studied by destructive extraction of compounds from plant tissues for in vitro analyses. The methods presented here utilize the green fluorescent protein (gfp) gene for continual monitoring of gene expression in the same pieces of tissues, over time. The gfp gene was placed under regulatory control of different promoters and introduced into lima bean cotyledonary tissues via particle bombardment. Cotyledons were then placed on a robotic image collection system, which consisted of a fluorescence dissecting microscope with a digital camera and a 2-dimensional robotics platform custom-designed to allow secure attachment of culture dishes. Images were collected from cotyledonary tissues every hour for 100 hours to generate expression profiles for each promoter. Each collected series of 100 images was first subjected to manual image alignment using ImageReady to make certain that GFP-expressing foci were consistently retained within selected fields of analysis. Specific regions of the series measuring 300 x 400 pixels, were then selected for further analysis to provide GFP Intensity measurements using ImageJ software. Batch images were separated into the red, green and blue channels and GFP-expressing areas were identified using the threshold feature of ImageJ. After subtracting the background fluorescence (subtraction of gray values of non-expressing pixels from every pixel) in the respective red and green channels, GFP intensity was calculated by multiplying the mean grayscale value per pixel by the total number of GFP-expressing pixels in each channel, and then adding those values for both the red and green channels. GFP Intensity values were collected for all 100 time points to yield expression profiles. Variations in GFP expression profiles resulted from differences in factors such as promoter strength, presence of a silencing suppressor, or nature of the promoter. In addition to quantification of GFP intensity, the

Metrically preserving the USGS aerial film archive

USGS Publications Warehouse

Moe, Donald; Longhenry, Ryan

2013-01-01

Since 1972, the U.S. Geological Survey (USGS) Earth Resources Observation and Science (EROS) Center in Sioux Falls, South Dakota, has provided fi lm-based products to the public. EROS is home to an archive of 12 million frames of analog photography ranging from 1937 to the present. The archive contains collections from both aerial and satellite platforms including programs such as the National High Altitude Program (NHAP), National Aerial Photography Program (NAPP), U.S. Antarctic Resource Center (USARC), Declass 1(CORONA, ARGON, and LANYARD), Declass 2 (KH-7 and KH-9), and Landsat (1972 – 1992, Landsat 1–5).

Mutagenic Activity of Indigofera truxillensis and I. suffruticosa Aerial Parts

PubMed Central

Calvo, Tamara Regina; Cardoso, Cássia Regina Primila; da Silva Moura, Adriana Candido; dos Santos, Lourdes Campaner; Colus, Ilce Mara Syllos; Vilegas, Wagner; Varanda, Eliana Aparecida

2011-01-01

Indigofera truxillensis and I. suffruticosa, are used as a source of indigo dye and to treat several diseases. The mutagenic activity of the methanolic extracts from aerial parts, glycerolipid, flavonoid and alkaloid fractions of the extract were evaluated by means of Salmonella/microsome assays using TA100, TA98, TA102 and TA97a strains. The methanolic extract of I. truxillensis showed mutagenic activity in the TA98 strain without S9 while glycerolipid fraction was devoid of activity. The flavonoid and alkaloid fractions of both plants showed mutagenicity. Chemical analysis of flavonoid fractions of I. truxillensis and I. suffruticosa resulted in the identification of kaempferol, quercetin and their derivatives. The alkaloid fraction of both the species contained indigo and indirubin and indigo was found mainly responsible for the mutagenic activity. PMID:19696193

FlyAR: augmented reality supported micro aerial vehicle navigation.

PubMed

Zollmann, Stefanie; Hoppe, Christof; Langlotz, Tobias; Reitmayr, Gerhard

2014-04-01

Micro aerial vehicles equipped with high-resolution cameras can be used to create aerial reconstructions of an area of interest. In that context automatic flight path planning and autonomous flying is often applied but so far cannot fully replace the human in the loop, supervising the flight on-site to assure that there are no collisions with obstacles. Unfortunately, this workflow yields several issues, such as the need to mentally transfer the aerial vehicle’s position between 2D map positions and the physical environment, and the complicated depth perception of objects flying in the distance. Augmented Reality can address these issues by bringing the flight planning process on-site and visualizing the spatial relationship between the planned or current positions of the vehicle and the physical environment. In this paper, we present Augmented Reality supported navigation and flight planning of micro aerial vehicles by augmenting the user’s view with relevant information for flight planning and live feedback for flight supervision. Furthermore, we introduce additional depth hints supporting the user in understanding the spatial relationship of virtual waypoints in the physical world and investigate the effect of these visualization techniques on the spatial understanding.

Unplanned releases and injuries associated with aerial application of chemicals, 1995-2002.

PubMed

Rice, Nancy; Messing, Rita; Souther, Larry; Berkowitz, Zahava

2005-11-01

For this article, records of the Hazardous Substances Emergency Events Surveillance (HSEES) system were reviewed to identify and describe acute, unplanned releases of agricultural chemicals and associated injuries related to aerial application during 1995-2002. Records of aerial-application accidents from the National Transportation Safety Board were also reviewed. Of the 54,090 events in the HSEES system for 1995-2002, 91 were identified as aerial-application events. The most commonly released substance was malathion. There were 56 victims; 12 died, and 34 required treatment at a hospital. A higher percentage of HSEES aerial-applicator events involved injury and death than did other HSEES transportation events. The relatively high number of injuries and fatalities underscores the need for precautions such as monitoring and limiting pilot cumulative exposures to pesticides, and using appropriate personal protective equipment and decontamination equipment. Emergency responders should be educated about the hazards associated with chemicals at aerial-application crash sites.

Microbial small molecules - weapons of plant subversion.

PubMed

Stringlis, Ioannis A; Zhang, Hao; Pieterse, Corné M J; Bolton, Melvin D; de Jonge, Ronnie

2018-05-25

Covering: up to 2018 Plants live in close association with a myriad of microbes that are generally harmless. However, the minority of microbes that are pathogens can severely impact crop quality and yield, thereby endangering food security. By contrast, beneficial microbes provide plants with important services, such as enhanced nutrient uptake and protection against pests and diseases. Like pathogens, beneficial microbes can modulate host immunity to efficiently colonize the nutrient-rich niches within and around the roots and aerial tissues of a plant, a phenomenon mirroring the establishment of commensal microbes in the human gut. Numerous ingenious mechanisms have been described by which pathogenic and beneficial microbes in the plant microbiome communicate with their host, including the delivery of immune-suppressive effector proteins and the production of phytohormones, toxins and other bioactive molecules. Plants signal to their associated microbes via exudation of photosynthetically fixed carbon sources, quorum-sensing mimicry molecules and selective secondary metabolites such as strigolactones and flavonoids. Molecular communication thus forms an integral part of the establishment of both beneficial and pathogenic plant-microbe relations. Here, we review the current knowledge on microbe-derived small molecules that can act as signalling compounds to stimulate plant growth and health by beneficial microbes on the one hand, but also as weapons for plant invasion by pathogens on the other. As an exemplary case, we used comparative genomics to assess the small molecule biosynthetic capabilities of the Pseudomonas genus; a genus rich in both plant pathogenic and beneficial microbes. We highlight the biosynthetic potential of individual microbial genomes and the population at large, providing evidence for the hypothesis that the distinction between detrimental and beneficial microbes is increasingly fading. Knowledge on the biosynthesis and molecular activity of

Using high-resolution digital aerial imagery to map land cover

USGS Publications Warehouse

Dieck, J.J.; Robinson, Larry

2014-01-01

The Upper Midwest Environmental Sciences Center (UMESC) has used aerial photography to map land cover/land use on federally owned and managed lands for over 20 years. Until recently, that process used 23- by 23-centimeter (9- by 9-inch) analog aerial photos to classify vegetation along the Upper Mississippi River System, on National Wildlife Refuges, and in National Parks. With digital aerial cameras becoming more common and offering distinct advantages over analog film, UMESC transitioned to an entirely digital mapping process in 2009. Though not without challenges, this method has proven to be much more accurate and efficient when compared to the analog process.

Presence of amphibian chytrid fungus (Batrachochytrium dendrobatidis) in rainwater suggests aerial dispersal is possible

USGS Publications Warehouse

Kolby, Jonathan E.; Sara D. Ramirez,; Lee Berger,; Griffin, Dale W.; Merlijn Jocque,; Lee F. Skerratt,

2015-01-01

Abstract Global spread of the pathogenic amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) may involve dispersal mechanisms not previously explored. Weather systems accompanied by strong wind and rainfall have been known to assist the dispersal of microbes pathogenic to plants and animals, and we considered a similar phenomenon might occur with Bd. We investigated this concept by sampling rainwater from 20 precipitation events for the presence of Bd in Cusuco National Park, Honduras: a site where high Bd prevalence was previously detected in stream-associated amphibians. Quantitative PCR analysis confirmed the presence of Bd in rainwater in one (5 %) of the weather events sampled, although viability cannot be ascertained from molecular presence alone. The source of the Bd and distance that the contaminated rainwater traveled could not be determined; however, this collection site was located approximately 600 m from the nearest observed perennial river by straight-line aerial distance. Although our results suggest atmospheric Bd dispersal is uncommon and unpredictable, even occasional short-distance aerial transport could considerably expand the taxonomic diversity of amphibians vulnerable to exposure and at risk of decline, including terrestrial and arboreal species that are not associated with permanent water bodies.

The Joint Tactical Aerial Resupply Vehicle Impact on Sustainment Operations

DTIC Science & Technology

2017-06-09

Artificial Intelligence , Sustainment Operations, Rifle Company, Autonomous Aerial Resupply, Joint Tactical Autonomous Aerial Resupply System 16...Integrations and Development System AI Artificial Intelligence ARCIC Army Capabilities Integration Center ARDEC Armament Research, Development and...semi- autonomous systems, and fully autonomous systems. Autonomy of machines depends on sophisticated software, including Artificial Intelligence

Kite Aerial Photography (KAP) as a Tool for Field Teaching

ERIC Educational Resources Information Center

Sander, Lasse

2014-01-01

Kite aerial photography (KAP) is proposed as a creative tool for geography field teaching and as a medium to approach the complexity of readily available geodata. The method can be integrated as field experiment, surveying technique or group activity. The acquired aerial images can instantaneously be integrated in geographic information systems…

Aerial photo interpretation of understories in two Oregon oak stands.

Treesearch

H. Gyde Lund; George R. Fahnestock; John F. Wear

1967-01-01

Aerial color photography has shown promise for evaluating understory vegetation as a forest-fire fuel. Mapping understory vegetation from special aerial photography produced results reasonably similar to those obtained by an independent ground check. Differences in the methods used in the exploratory work prevented strict comparability, but agreement was close enough...

Locating waterfowl observations on aerial surveys

USGS Publications Warehouse

Butler, W.I.; Hodges, J.I.; Stehn, R.A.

1995-01-01

We modified standard aerial survey data collection to obtain the geographic location for each waterfowl observation on surveys in Alaska during 1987-1993. Using transect navigation with CPS (global positioning system), data recording on continuously running tapes, and a computer data input program, we located observations with an average deviation along transects of 214 m. The method provided flexibility in survey design and data analysis. Although developed for geese nesting near the coast of the Yukon-Kuskokwim Delta, the methods are widely applicable and were used on other waterfowl surveys in Alaska to map distribution and relative abundance of waterfowl. Accurate location data with GIS analysis and display may improve precision and usefulness of data from any aerial transect survey.

Aerospace toxicology overview: aerial application and cabin air quality.

PubMed

Chaturvedi, Arvind K

2011-01-01

Aerospace toxicology is a rather recent development and is closely related to aerospace medicine. Aerospace toxicology can be defined as a field of study designed to address the adverse effects of medications, chemicals, and contaminants on humans who fly within or outside the atmosphere in aviation or on space flights. The environment extending above and beyond the surface of the Earth is referred to as aerospace. The term aviation is frequently used interchangeably with aerospace. The focus of the literature review performed to prepare this paper was on aerospace toxicology-related subject matters, aerial application and aircraft cabin air quality. Among the important topics addressed are the following: · Aerial applications of agricultural chemicals, pesticidal toxicity, and exposures to aerially applied mixtures of chemicals and their associated formulating solvents/surfactants The safety of aerially encountered chemicals and the bioanalytical methods used to monitor exposures to some of them · The presence of fumes and smoke, as well as other contaminants that may generally be present in aircraft/space vehicle cabin air · And importantly, the toxic effects of aerially encountered contaminants, with emphasis on the degradation products of oils, fluids, and lubricants used in aircraft, and finally · Analytical methods used for monitoring human exposure to CO and HCN are addressed in the review, as are the signs and symptoms associated with exposures to these combustion gases. Although many agricultural chemical monitoring studies have been published, few have dealt with the occurrence of such chemicals in aircraft cabin air. However, agricultural chemicals do appear in cabin air; indeed, attempts have been made to establish maximum allowable concentrations for several of the more potentially toxic ones that are found in aircraft cabin air. In this article, I emphasize the need for precautionary measures to be taken to minimize exposures to aerially

A Spherical Aerial Terrestrial Robot

NASA Astrophysics Data System (ADS)

Dudley, Christopher J.

This thesis focuses on the design of a novel, ultra-lightweight spherical aerial terrestrial robot (ATR). The ATR has the ability to fly through the air or roll on the ground, for applications that include search and rescue, mapping, surveillance, environmental sensing, and entertainment. The design centers around a micro-quadcopter encased in a lightweight spherical exoskeleton that can rotate about the quadcopter. The spherical exoskeleton offers agile ground locomotion while maintaining characteristics of a basic aerial robot in flying mode. A model of the system dynamics for both modes of locomotion is presented and utilized in simulations to generate potential trajectories for aerial and terrestrial locomotion. Details of the quadcopter and exoskeleton design and fabrication are discussed, including the robot's turning characteristic over ground and the spring-steel exoskeleton with carbon fiber axle. The capabilities of the ATR are experimentally tested and are in good agreement with model-simulated performance. An energy analysis is presented to validate the overall efficiency of the robot in both modes of locomotion. Experimentally-supported estimates show that the ATR can roll along the ground for over 12 minutes and cover the distance of 1.7 km, or it can fly for 4.82 minutes and travel 469 m, on a single 350 mAh battery. Compared to a traditional flying-only robot, the ATR traveling over the same distance in rolling mode is 2.63-times more efficient, and in flying mode the system is only 39 percent less efficient. Experimental results also demonstrate the ATR's transition from rolling to flying mode.

An Improved Aerial Target Localization Method with a Single Vector Sensor

PubMed Central

Zhao, Anbang; Bi, Xuejie; Hui, Juan; Zeng, Caigao; Ma, Lin

2017-01-01

This paper focuses on the problems encountered in the actual data processing with the use of the existing aerial target localization methods, analyzes the causes of the problems, and proposes an improved algorithm. Through the processing of the sea experiment data, it is found that the existing algorithms have higher requirements for the accuracy of the angle estimation. The improved algorithm reduces the requirements of the angle estimation accuracy and obtains the robust estimation results. The closest distance matching estimation algorithm and the horizontal distance estimation compensation algorithm are proposed. The smoothing effect of the data after being post-processed by using the forward and backward two-direction double-filtering method has been improved, thus the initial stage data can be filtered, so that the filtering results retain more useful information. In this paper, the aerial target height measurement methods are studied, the estimation results of the aerial target are given, so as to realize the three-dimensional localization of the aerial target and increase the understanding of the underwater platform to the aerial target, so that the underwater platform has better mobility and concealment. PMID:29135956

Aerial surveillance vehicles augment security at shipping ports

NASA Astrophysics Data System (ADS)

Huck, Robert C.; Al Akkoumi, Muhammad K.; Cheng, Samuel; Sluss, James J., Jr.; Landers, Thomas L.

2008-10-01

With the ever present threat to commerce, both politically and economically, technological innovations provide a means to secure the transportation infrastructure that will allow efficient and uninterrupted freight-flow operations for trade. Currently, freight coming into United States ports is "spot checked" upon arrival and stored in a container yard while awaiting the next mode of transportation. For the most part, only fences and security patrols protect these container storage yards. To augment these measures, the authors propose the use of aerial surveillance vehicles equipped with video cameras and wireless video downlinks to provide a birds-eye view of port facilities to security control centers and security patrols on the ground. The initial investigation described in this paper demonstrates the use of unmanned aerial surveillance vehicles as a viable method for providing video surveillance of container storage yards. This research provides the foundation for a follow-on project to use autonomous aerial surveillance vehicles coordinated with autonomous ground surveillance vehicles for enhanced port security applications.

Aerial Observation Needs Workshop, May 13-14, 2015

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nasiri, Shaima; Serbin, Shawn; Lesmes, David

2015-10-01

The mission of the Climate and Environmental Sciences Division (CESD) of the Office of Biological and Environmental Research (BER) within the U.S. Department of Energy's (DOE) Office of Science is "to advance a robust, predictive understanding of Earth's climate and environmental systems and to inform the development of sustainable solutions to the nation's energy and environmental challenges." Accomplishing this mission requires aerial observations of the atmospheric and terrestrial components of the climate system. CESD is assessing its current and future aerial observation needs to develop a strategy and roadmap of capability requirements for the next decade. To facilitate this process,more » a workshop was convened that consisted of invited experts in the atmospheric and terrestrial sciences, airborne observations, and modeling. This workshop report summarizes the community input prior to and during the workshop on research challenges and opportunities, as well as specific science questions and observational needs that require aerial observations to address.« less

Distinguishing plant population and variety with UAV-derived vegetation indices

NASA Astrophysics Data System (ADS)

Oakes, Joseph; Balota, Maria

2017-05-01

Variety selection and seeding rate are two important choice that a peanut grower must make. High yielding varieties can increase profit with no additional input costs, while seeding rate often determines input cost a grower will incur from seed costs. The overall purpose of this study was to examine the effect that seeding rate has on different peanut varieties. With the advent of new UAV technology, we now have the possibility to use indices collected with the UAV to measure emergence, seeding rate, growth rate, and perhaps make yield predictions. This information could enable growers to make management decisions early in the season based on low plant populations due to poor emergence, and could be a useful tool for growers to use to estimate plant population and growth rate in order to help achieve desired crop stands. Red-Green-Blue (RGB) and near-infrared (NIR) images were collected from a UAV platform starting two weeks after planting and continued weekly for the next six weeks. Ground NDVI was also collected each time aerial images were collected. Vegetation indices were derived from both the RGB and NIR images. Greener area (GGA- the proportion of green pixels with a hue angle from 80° to 120°) and a* (the average red/green color of the image) were derived from the RGB images while Normalized Differential Vegetative Index (NDVI) was derived from NIR images. Aerial indices were successful in distinguishing seeding rates and determining emergence during the first few weeks after planting, but not later in the season. Meanwhile, these aerial indices are not an adequate predictor of yield in peanut at this point.

The Role of Unmanned Aerial Systems-Sensors in Air Quality Research

EPA Science Inventory

The use of unmanned aerial systems (UASs) and miniaturized sensors for a variety of scientific and security purposes has rapidly increased. UASs include aerostats (tethered balloons) and remotely controlled, unmanned aerial vehicles (UAVs) including lighter-than-air vessels, fix...

Evaluation of Three Protein-Extraction Methods for Proteome Analysis of Maize Leaf Midrib, a Compound Tissue Rich in Sclerenchyma Cells.

PubMed

Wang, Ning; Wu, Xiaolin; Ku, Lixia; Chen, Yanhui; Wang, Wei

2016-01-01

Leaf morphology is closely related to the growth and development of maize (Zea mays L.) plants and final kernel production. As an important part of the maize leaf, the midrib holds leaf blades in the aerial position for maximum sunlight capture. Leaf midribs of adult plants contain substantial sclerenchyma cells with heavily thickened and lignified secondary walls and have a high amount of phenolics, making protein extraction and proteome analysis difficult in leaf midrib tissue. In the present study, three protein-extraction methods that are commonly used in plant proteomics, i.e., phenol extraction, TCA/acetone extraction, and TCA/acetone/phenol extraction, were qualitatively and quantitatively evaluated based on 2DE maps and MS/MS analysis using the midribs of the 10th newly expanded leaves of maize plants. Microscopy revealed the existence of substantial amounts of sclerenchyma underneath maize midrib epidermises (particularly abaxial epidermises). The spot-number order obtained via 2DE mapping was as follows: phenol extraction (655) > TCA/acetone extraction (589) > TCA/acetone/phenol extraction (545). MS/MS analysis identified a total of 17 spots that exhibited 2-fold changes in abundance among the three methods (using phenol extraction as a control). Sixteen of the proteins identified were hydrophilic, with GRAVY values ranging from -0.026 to -0.487. For all three methods, we were able to obtain high-quality protein samples and good 2DE maps for the maize leaf midrib. However, phenol extraction produced a better 2DE map with greater resolution between spots, and TCA/acetone extraction produced higher protein yields. Thus, this paper includes a discussion regarding the possible reasons for differential protein extraction among the three methods. This study provides useful information that can be used to select suitable protein extraction methods for the proteome analysis of recalcitrant plant tissues that are rich in sclerenchyma cells.

Cold Tolerance of Plants Used for Cold-Regions Revegetation

DTIC Science & Technology

1990-10-01

water Mitochondrial 02 uptake Sweet potatot Decreased translocation, which can result in the desiccation of Chlorogenic acid Sweet potato Increased...Amino acids Bean Increased of toxic substances between the ice and the soil surface. Protein Bean Decreased Also, frozen soil and plant stems can prevent...warmer aerial plant parts. Oxalic acid Oxalis sp. Increased Chlorophyll Bean Decreased Frost heaving has been a concern in forestry and Organic acids

The Role of Unmanned Aerial Systems/Sensors in Air Quality Research

EPA Science Inventory

The use of unmanned aerial systems (UASs) for a variety of scientific and security purposes has rapidly increased. UASs include aerostats (tethered balloons) and remotely controlled, unmanned aerial vehicles (UAVs) including lighter-than-air vessels, fixed wing airplanes, and he...

Current status and future directions of precision agriculture for aerial application in the USA

USDA-ARS?s Scientific Manuscript database

Precision aerial application in the USA is less than a decade old since the development of the first variable-rate aerial application system. Many areas of the United States rely on readily available agricultural airplanes or helicopters for pest management. Variable-rate aerial application provides...

Interactions between Streptomyces coelicolor and Bacillus subtilis: Role of surfactants in raising aerial structures.

PubMed

Straight, Paul D; Willey, Joanne M; Kolter, Roberto

2006-07-01

Using mixed-species cultures, we have undertaken a study of interactions between two common spore-forming soil bacteria, Bacillus subtilis and Streptomyces coelicolor. Our experiments demonstrate that the development of aerial hyphae and spores by S. coelicolor is inhibited by surfactin, a lipopeptide surfactant produced by B. subtilis. Current models of aerial development by sporulating bacteria and fungi postulate a role for surfactants in reducing surface tension at air-liquid interfaces, thereby removing the major barrier to aerial growth. S. coelicolor produces SapB, an amphipathic peptide that is surface active and required for aerial growth on certain media. Loss of aerial hyphae in developmental mutants can be rescued by addition of purified SapB. While a surfactant from a fungus can substitute for SapB in a mutant that lacks aerial hyphae, not all surfactants have this effect. We show that surfactin is required for formation of aerial structures on the surface of B. subtilis colonies. However, in contrast to this positive role, our experiments reveal that surfactin acts antagonistically by arresting S. coelicolor aerial development and causing altered expression of developmental genes. Our observations support the idea that surfactants function specifically for a given organism regardless of their shared ability to reduce surface tension. Production of surfactants with antagonistic activity could provide a powerful competitive advantage during surface colonization and in competition for resources.

Remotely deployable aerial inspection using tactile sensors

DOE Office of Scientific and Technical Information (OSTI.GOV)

MacLeod, C. N.; Cao, J.; Pierce, S. G.

For structural monitoring applications, the use of remotely deployable Non-Destructive Evaluation (NDE) inspection platforms offer many advantages, including improved accessibility, greater safety and reduced cost, when compared to traditional manual inspection techniques. The use of such platforms, previously reported by researchers at the University Strathclyde facilitates the potential for rapid scanning of large areas and volumes in hazardous locations. A common problem for both manual and remote deployment approaches lies in the intrinsic stand-off and surface coupling issues of typical NDE probes. The associated complications of these requirements are obviously significantly exacerbated when considering aerial based remote inspection and deployment,more » resulting in simple visual techniques being the preferred sensor payload. Researchers at Bristol Robotics Laboratory have developed biomimetic tactile sensors modelled on the facial whiskers (vibrissae) of animals such as rats and mice, with the latest sensors actively sweeping their tips across the surface in a back and forth motion. The current work reports on the design and performance of an aerial inspection platform and the suitability of tactile whisking sensors to aerial based surface monitoring applications.« less

Verification of aerial photo stand volume tables for southeast Alaska.

Treesearch

Theodore S. Setzer; Bert R. Mead

1988-01-01

Aerial photo volume tables are used in the multilevel sampling system of Alaska Forest Inventory and Analysis. These volume tables are presented with a description of the data base and methods used to construct the tables. Volume estimates compiled from the aerial photo stand volume tables and associated ground-measured values are compared and evaluated.

An electrochemical approach to monitor pH change in agar media during plant tissue culture.

PubMed

Wang, Min; Ha, Yang

2007-05-15

In this work, metal oxide microelectrodes were developed to monitor pH change in agar media during plant tissue culture. An antimony wire was produced by a new approach "capillary melt method". The surface of the obtained antimony wire was oxidized in a potassium nitrate melt to fabricate an antimony oxide film for pH sensing. Characterization results show that the oxide layer grown on the wire surface consists of Sb(2)O(3) crystal phase. The sensing response, open-circuit potential, of the electrode has a good linear relationship (R(2)=1.00) with pH value of the test solution. Adding organic compounds into the test media would not affect the linear relationship, although the slope of the lines varied with different ingredients added. The antimony oxide electrodes were employed to continuously monitor pH change of agar culture media during a 2-week plant tissue culture of Dendrobium candidum. The antimony oxide electrode fabricated this way has the advantages of low cost, easy fabrication, fast response, and almost no contamination introduced into the system. It would be suitable for in situ and continuous pH measurement in many bio applications.

Unmanned Aerial Vehicles Produce High-Resolution Seasonally-Relevant Imagery for Classifying Wetland Vegetation

NASA Astrophysics Data System (ADS)

Marcaccio, J. V.; Markle, C. E.; Chow-Fraser, P.

2015-08-01

With recent advances in technology, personal aerial imagery acquired with unmanned aerial vehicles (UAVs) has transformed the way ecologists can map seasonal changes in wetland habitat. Here, we use a multi-rotor (consumer quad-copter, the DJI Phantom 2 Vision+) UAV to acquire a high-resolution (< 8 cm) composite photo of a coastal wetland in summer 2014. Using validation data collected in the field, we determine if a UAV image and SWOOP (Southwestern Ontario Orthoimagery Project) image (collected in spring 2010) differ in their classification of type of dominant vegetation type and percent cover of three plant classes: submerged aquatic vegetation, floating aquatic vegetation, and emergent vegetation. The UAV imagery was more accurate than available SWOOP imagery for mapping percent cover of submergent and floating vegetation categories, but both were able to accurately determine the dominant vegetation type and percent cover of emergent vegetation. Our results underscore the value and potential for affordable UAVs (complete quad-copter system < 3,000 CAD) to revolutionize the way ecologists obtain imagery and conduct field research. In Canada, new UAV regulations make this an easy and affordable way to obtain multiple high-resolution images of small (< 1.0 km2) wetlands, or portions of larger wetlands throughout a year.

High Density Aerial Image Matching: State-Of and Future Prospects

NASA Astrophysics Data System (ADS)

Haala, N.; Cavegn, S.

2016-06-01

Ongoing innovations in matching algorithms are continuously improving the quality of geometric surface representations generated automatically from aerial images. This development motivated the launch of the joint ISPRS/EuroSDR project "Benchmark on High Density Aerial Image Matching", which aims on the evaluation of photogrammetric 3D data capture in view of the current developments in dense multi-view stereo-image matching. Originally, the test aimed on image based DSM computation from conventional aerial image flights for different landuse and image block configurations. The second phase then put an additional focus on high quality, high resolution 3D geometric data capture in complex urban areas. This includes both the extension of the test scenario to oblique aerial image flights as well as the generation of filtered point clouds as additional output of the respective multi-view reconstruction. The paper uses the preliminary outcomes of the benchmark to demonstrate the state-of-the-art in airborne image matching with a special focus of high quality geometric data capture in urban scenarios.

Modelling and Closed-Loop System Identification of a Quadrotor-Based Aerial Manipulator

NASA Astrophysics Data System (ADS)

Dube, Chioniso; Pedro, Jimoh O.

2018-05-01

This paper presents the modelling and system identification of a quadrotor-based aerial manipulator. The aerial manipulator model is first derived analytically using the Newton-Euler formulation for the quadrotor and Recursive Newton-Euler formulation for the manipulator. The aerial manipulator is then simulated with the quadrotor under Proportional Derivative (PD) control, with the manipulator in motion. The simulation data is then used for system identification of the aerial manipulator. Auto Regressive with eXogenous inputs (ARX) models are obtained from the system identification for linear accelerations \\ddot{X} and \\ddot{Y} and yaw angular acceleration \\ddot{\\psi }. For linear acceleration \\ddot{Z}, and pitch and roll angular accelerations \\ddot{θ } and \\ddot{φ }, Auto Regressive Moving Average with eXogenous inputs (ARMAX) models are identified.

ESTIMATING DENSITIES OF ESTUARINE EELGRASS AND BENTHIC MACROALGAE VIA AERIAL PHOTOGRAPHY

EPA Science Inventory

The goal of this research was to distinguish meadows of native eelgrass (Zostera marina L.) from adjacent beds of benthic green macroalgae in the exposed intertidal zone of Pacific Northwest estuaries, using false-color near-infrared aerial photography. Aerial photographs of Yaq...

Development and prospect of unmanned aerial vehicles for agricultural production management

USDA-ARS?s Scientific Manuscript database

Unmanned aerial vehicles have been developed and applied to support agricultural production management. Compared to piloted aircrafts, an Unmanned Aerial Vehicle (UAV) can focus on small crop fields in lower flight altitude than regular airplanes to perform site-specific management with high precisi...

The Ground Control Room as an Enabling Technology in the Unmanned Aerial System

NASA Technical Reports Server (NTRS)

Gear, Gary; Mace, Thomas

2007-01-01

This viewgraph presentation reviews the development of the ground control room as an required technology for the use of an Unmanned Aerial system. The Unmanned Aerial system is a strategic component of the Global Observing System, which will serve global science needs. The unmanned aerial system will use the same airspace as manned aircraft, therefore there will be unique telemetry needs.

CMOS Imaging Sensor Technology for Aerial Mapping Cameras

NASA Astrophysics Data System (ADS)

Neumann, Klaus; Welzenbach, Martin; Timm, Martin

2016-06-01

In June 2015 Leica Geosystems launched the first large format aerial mapping camera using CMOS sensor technology, the Leica DMC III. This paper describes the motivation to change from CCD sensor technology to CMOS for the development of this new aerial mapping camera. In 2002 the DMC first generation was developed by Z/I Imaging. It was the first large format digital frame sensor designed for mapping applications. In 2009 Z/I Imaging designed the DMC II which was the first digital aerial mapping camera using a single ultra large CCD sensor to avoid stitching of smaller CCDs. The DMC III is now the third generation of large format frame sensor developed by Z/I Imaging and Leica Geosystems for the DMC camera family. It is an evolution of the DMC II using the same system design with one large monolithic PAN sensor and four multi spectral camera heads for R,G, B and NIR. For the first time a 391 Megapixel large CMOS sensor had been used as PAN chromatic sensor, which is an industry record. Along with CMOS technology goes a range of technical benefits. The dynamic range of the CMOS sensor is approx. twice the range of a comparable CCD sensor and the signal to noise ratio is significantly better than with CCDs. Finally results from the first DMC III customer installations and test flights will be presented and compared with other CCD based aerial sensors.

Genetic Variation in an Inbred Plant: Variation in Tissue Cultures of Soybean [Glycine Max (L.) Merrill

PubMed Central

Roth, E. J.; Frazier, B. L.; Apuya, N. R.; Lark, K. G.

1989-01-01

Although soybean [Glycine max (L.) Merrill] grows as an inbreeding, generally homozygous, plant, the germplasm of the species contains large amounts of genetic variation. Analysis of soybean DNA has indicated that variation of RFLP (restriction fragment length polymorphism) markers within the species usually entails only two alleles at any one locus and that mixtures of such dimorphic loci account for virtually all of the restriction fragment variation seen in soybean (G. max), and in its ancestors, G. soja and G. gracilis. We report here that tissue cultures prepared from root tissue of individual soybean plants develop RFLP allelic differences at various loci. However, these newly generated alleles are almost always the same as ones previously found and characterized in other varieties of cultivated soybean (cultivars). This repeated generation of particular alleles suggests that much of the genetic variation seen in soybean could be the consequence of specific, relatively frequently employed, recombinational events. Such a mechanism would allow inbred cultivars to generate genetic variation (in the form of alternative alleles) in a controlled manner, perhaps in response to stress. PMID:2567263

Three scales of aerial photography compared for making stand measurements

Treesearch

Earl J. Rogers; Gene Avery; Roy A. Chapman

1959-01-01

Three scales of aerial photography were tested in an attempt to determine the best scale to use in forest surveying. This was done by comparing photo measurements of average tree height, average crown diameter, and crown-closure percent. These stand variables were selected for testing because of their applicability in making aerial estimates of timber volume.

Suitability of low cost commercial off-the-shelf aerial platforms and consumer grade digital cameras for small format aerial photography

NASA Astrophysics Data System (ADS)

Turley, Anthony Allen

Many research projects require the use of aerial images. Wetlands evaluation, crop monitoring, wildfire management, environmental change detection, and forest inventory are but a few of the applications of aerial imagery. Low altitude Small Format Aerial Photography (SFAP) is a bridge between satellite and man-carrying aircraft image acquisition and ground-based photography. The author's project evaluates digital images acquired using low cost commercial digital cameras and standard model airplanes to determine their suitability for remote sensing applications. Images from two different sites were obtained. Several photo missions were flown over each site, acquiring images in the visible and near infrared electromagnetic bands. Images were sorted and analyzed to select those with the least distortion, and blended together with Microsoft Image Composite Editor. By selecting images taken within minutes apart, radiometric qualities of the images were virtually identical, yielding no blend lines in the composites. A commercial image stitching program, Autopano Pro, was purchased during the later stages of this study. Autopano Pro was often able to mosaic photos that the free Image Composite Editor was unable to combine. Using telemetry data from an onboard data logger, images were evaluated to calculate scale and spatial resolution. ERDAS ER Mapper and ESRI ArcGIS were used to rectify composite images. Despite the limitations inherent in consumer grade equipment, images of high spatial resolution were obtained. Mosaics of as many as 38 images were created, and the author was able to record detailed aerial images of forest and wetland areas where foot travel was impractical or impossible.

Collecting maple sap with unvented spouts, using aerial and ground lines

Treesearch

H. Clay Smith; Carter B. Gibbs

1971-01-01

Two methods of using plastic tubing to collect sugar maple sap were tried: aerial lines and ground lines. Unvented spouts were used in both. We found that the sap yields collected from the aerial and ground lines were not statistically different from each other.

Development of Unmanned Aerial Vehicles for Site-Specific Crop Production Management

USDA-ARS?s Scientific Manuscript database

Unmanned Aerial Vehicles (UAV) have been developed and applied to support the practice of precision agriculture. Compared to piloted aircrafts, an Unmanned Aerial Vehicle can focus on much smaller crop fields with much lower flight altitude than regular airplanes to perform site-specific management ...

Medicinal plants used for ophthalmological problems in Navarra (Spain).

PubMed

Calvo, M Isabel; Cavero, Rita Yolanda

2016-08-22

Several plants have been found to have effective against number of ophthalmological problems in Navarra. Information was collected using semi-structured ethnobotanical interviews with 686 informants in 267 locations. In order to confirm the pharmacological application of the uses more cited by the informants, a literature review was conducted. A total of 57 pharmaceutical uses were reported, for 19 plants and 13 families, mainly represented by Asteraceae. The most frequently used parts were inflorescences, flowers, aerial parts, leaves and flowered aerial parts. The related affections fell into eleven categories: bloodshot eyes, watery eyes and wounds, improve vision, irrited eyelids, rheums and styes, tired eyes, conjunctivitis, eyewash, ocular problems in general. The most cited plants were: Chamaemelum nobile (L.) All., Santolina chamaecyparissus L. ssp. squarrosa (DC.) Nyman, Sambucus nigra L. ssp. nigra, Rosa agrestis Savi and Calendula officinalis L. None of them have been pharmacologically validated by Official International Organisms. From the therapeutic point of view, Allium sativum L., Foeniculum vulgare Mill., C. officinalis, and S. chamaecyparissus ssp. squarrosa deserve special attention, because ethnobotanical and pharmacological studies suggest that these medicinal plants are effective for ophthalmological problems. The present study constitutes a good basis for further phytochemical and pharmacological research of these four plants, which could be of interest in the design of new inexpensive, effective and safe drugs. The remaining plants are needed to be screened through standard pharmacological and clinical procedures for their activities. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Phytochemical and Antioxidant Investigation of the Aerial Parts of Dorema glabrum Fisch. & C.A. Mey.

PubMed Central

Delnavazi, Mohammad-Reza; Hadjiakhoondi, Abbas; Delazar, Abbas; Ajani, Yousef; Tavakoli, Saeed; Yassa, Narguess

2015-01-01

Dorema glabrum Fisch. & C.A. Mey. (Apiaceae) is a monocarpic perennial plant distributed in southern Caucasus. In Azerbaijan Republic folk medicine, the gum-resin of this species is used as a diuretic and anti-diarrheal agent. It is also traditionally used for the treatment of bronchitis and catarrh. In the present study, chemical constituents of the essential oil and extract of D. glabrum aerial parts were investigated and their free radical scavenging potentials were assessed. GC-MS and GC-FID analyses of the plant essential oil resulted in identifying twenty compounds, out of which elemicin (38.6%) and myristicin (14.3%) were main compounds. Seven compounds including daucosterol (1), chlorogenic acid (2), a mixture of cynarin (3) and 3,5-di-O-caffeoylquinic acid (4), isorhamnetin-3-O-β-D-glucopyranoside (5), isoquercetin (6) and astragalin (7) were also isolated from the ethyl acetate and methanol fractions of D. glabrum aerial parts using different chromatographic methods on silica gel (normal and reversed-phase) and sephadex LH20. Structures of the isolated compounds were elucidated using UV and 1H, 13C-NMR spectrain comparison with those reported in respective published data. Antioxidant activities of the crude extract, fractions and isolated compounds were evaluated using DPPH free radical scavenging assay method. Among the fractions, methanol fraction (IC50=53.3 ±4.7μg mL-1) and among the isolated compounds, caffeoylquinic acid derivatives exhibited the highest free radical scavenging activity (IC50= 2.2-2.6 μg mL-1). PMID:26330882

Pb-induced responses in Zygophyllum fabago plants are organ-dependent and modulated by salicylic acid.

PubMed

López-Orenes, Antonio; Martínez-Pérez, Ascensión; Calderón, Antonio A; Ferrer, María A

2014-11-01

Zygophyllum fabago is a promising species for restoring heavy metal (HM) polluted soils, although the mechanisms involved in HM tolerance in this non-model plant remain largely unknown. This paper analyses the extent to which redox-active compounds and enzymatic antioxidants in roots, stems and leaves are responsible for Pb tolerance in a metallicolous ecotype of Z. fabago and the possible influence of salicylic acid (SA) pretreatment (24 h, 0.5 mM SA) in the response to Pb stress. SA pretreatment reduced both the accumulation of Pb in roots and even more so the concentration of Pb in aerial parts of the plants, although a similar drop in the content of chlorophylls and in the maximum quantum yield of photosystem II was observed in both Pb- and SA-Pb-treated plants. Pb increased the endogenous free SA levels in all organs and this response was enhanced in root tissues upon SA pretreatment. Generally, Pb induced a reduction in catalase, ascorbate peroxidase and glutathione reductase specific activities, whereas dehydroascorbate reductase was increased in all organs of control plants. SA pretreatment enhanced the Pb-induced H2O2 accumulation in roots by up-regulating Fe-superoxide dismutase isoenzymes. Under Pb stress, the GSH redox ratio remained highly reduced in all organs while the ascorbic acid redox ratio dropped in leaf tissues where a rise in lipid peroxidation products and electrolyte leakage was observed. Finally, an organ-dependent accumulation of proline and β-carboline alkaloids was found, suggesting these nitrogen-redox-active compounds could play a role in the adaptation strategies of this species to Pb stress. Copyright © 2014. Published by Elsevier Masson SAS.