Science.gov

Sample records for alternative gene programs

  1. A dynamic alternative splicing program regulates gene expression during terminal erythropoiesis

    PubMed Central

    Pimentel, Harold; Parra, Marilyn; Gee, Sherry; Ghanem, Dana; An, Xiuli; Li, Jie; Mohandas, Narla; Pachter, Lior; Conboy, John G.

    2014-01-01

    Alternative pre-messenger RNA splicing remodels the human transcriptome in a spatiotemporal manner during normal development and differentiation. Here we explored the landscape of transcript diversity in the erythroid lineage by RNA-seq analysis of five highly purified populations of morphologically distinct human erythroblasts, representing the last four cell divisions before enucleation. In this unique differentiation system, we found evidence of an extensive and dynamic alternative splicing program encompassing genes with many diverse functions. Alternative splicing was particularly enriched in genes controlling cell cycle, organelle organization, chromatin function and RNA processing. Many alternative exons exhibited differentiation-associated switches in splicing efficiency, mostly in late-stage polychromatophilic and orthochromatophilic erythroblasts, in concert with extensive cellular remodeling that precedes enucleation. A subset of alternative splicing switches introduces premature translation termination codons into selected transcripts in a differentiation stage-specific manner, supporting the hypothesis that alternative splicing-coupled nonsense-mediated decay contributes to regulation of erythroid-expressed genes as a novel part of the overall differentiation program. We conclude that a highly dynamic alternative splicing program in terminally differentiating erythroblasts plays a major role in regulating gene expression to ensure synthesis of appropriate proteome at each stage as the cells remodel in preparation for production of mature red cells. PMID:24442673

  2. A dynamic alternative splicing program regulates gene expression during terminal erythropoiesis.

    PubMed

    Pimentel, Harold; Parra, Marilyn; Gee, Sherry; Ghanem, Dana; An, Xiuli; Li, Jie; Mohandas, Narla; Pachter, Lior; Conboy, John G

    2014-04-01

    Alternative pre-messenger RNA splicing remodels the human transcriptome in a spatiotemporal manner during normal development and differentiation. Here we explored the landscape of transcript diversity in the erythroid lineage by RNA-seq analysis of five highly purified populations of morphologically distinct human erythroblasts, representing the last four cell divisions before enucleation. In this unique differentiation system, we found evidence of an extensive and dynamic alternative splicing program encompassing genes with many diverse functions. Alternative splicing was particularly enriched in genes controlling cell cycle, organelle organization, chromatin function and RNA processing. Many alternative exons exhibited differentiation-associated switches in splicing efficiency, mostly in late-stage polychromatophilic and orthochromatophilic erythroblasts, in concert with extensive cellular remodeling that precedes enucleation. A subset of alternative splicing switches introduces premature translation termination codons into selected transcripts in a differentiation stage-specific manner, supporting the hypothesis that alternative splicing-coupled nonsense-mediated decay contributes to regulation of erythroid-expressed genes as a novel part of the overall differentiation program. We conclude that a highly dynamic alternative splicing program in terminally differentiating erythroblasts plays a major role in regulating gene expression to ensure synthesis of appropriate proteome at each stage as the cells remodel in preparation for production of mature red cells. PMID:24442673

  3. Alternative Programming for Adults.

    ERIC Educational Resources Information Center

    Flint, Thomas A.; Frey, Ruth

    2003-01-01

    The Council for Adult and Experiential Learning is currently cataloguing alternative programming features that are most effective with adult students in a best practices inventory organized around a framework of high-level descriptive principles of effectiveness. This chapter identifies a few interesting features from a quick survey of this…

  4. Integrating alternative splicing detection into gene prediction

    PubMed Central

    Foissac, Sylvain; Schiex, Thomas

    2005-01-01

    Background Alternative splicing (AS) is now considered as a major actor in transcriptome/proteome diversity and it cannot be neglected in the annotation process of a new genome. Despite considerable progresses in term of accuracy in computational gene prediction, the ability to reliably predict AS variants when there is local experimental evidence of it remains an open challenge for gene finders. Results We have used a new integrative approach that allows to incorporate AS detection into ab initio gene prediction. This method relies on the analysis of genomically aligned transcript sequences (ESTs and/or cDNAs), and has been implemented in the dynamic programming algorithm of the graph-based gene finder EuGÈNE. Given a genomic sequence and a set of aligned transcripts, this new version identifies the set of transcripts carrying evidence of alternative splicing events, and provides, in addition to the classical optimal gene prediction, alternative optimal predictions (among those which are consistent with the AS events detected). This allows for multiple annotations of a single gene in a way such that each predicted variant is supported by a transcript evidence (but not necessarily with a full-length coverage). Conclusions This automatic combination of experimental data analysis and ab initio gene finding offers an ideal integration of alternatively spliced gene prediction inside a single annotation pipeline. PMID:15705189

  5. Alternative Work Programs

    ERIC Educational Resources Information Center

    Kuehn, Kerri L.

    2004-01-01

    Employers are feeling the strain of needing to offer alternative work arrangements to retain and recruit employees. Due to a change in demographics, dual-career couples and increased technology; people are demanding a transformation in the workplace environment. Two alternatives, which are being offered by employers, are flextime and…

  6. GATEWAYS: Degree program alternatives

    SciTech Connect

    Strong, K.R.; Thayer, M.M.

    1991-11-01

    Los Alamos National Laboratory is using non-traditional solutions to assist employees in attaining degrees required for essential Laboratory operations. Varieties of distance learning technologies have been implemented to meet some degree needs. While distance learning is not always a perfect solution, it enables the Laboratory to provide education that would otherwise not be practical for full-time employees. The Laboratory has also formed partnerships with local colleges to design programs to fill particular needs. Meeting the challenge of providing cost-effective, viable degree programs in an isolated location requires both patience and innovation.

  7. The Arapahoe Detention Alternatives Program.

    ERIC Educational Resources Information Center

    Illinois Univ., Champaign. Community Research Center.

    The efforts of the Colorado Division of Youth Services to develop a program that would reduce the inappropriate use of secure detention of juveniles by providing intake/screening units and various nonsecure alternative detention services are described. Program goals are discussed and criteria on which detention decisions were based for the…

  8. Evolution of alternative splicing after gene duplication.

    PubMed

    Su, Zhixi; Wang, Jianmin; Yu, Jun; Huang, Xiaoqiu; Gu, Xun

    2006-02-01

    Alternative splicing and gene duplication are two major sources of proteomic function diversity. Here, we study the evolutionary trend of alternative splicing after gene duplication by analyzing the alternative splicing differences between duplicate genes. We observed that duplicate genes have fewer alternative splice (AS) forms than single-copy genes, and that a negative correlation exists between the mean number of AS forms and the gene family size. Interestingly, we found that the loss of alternative splicing in duplicate genes may occur shortly after the gene duplication. These results support the subfunctionization model of alternative splicing in the early stage after gene duplication. Further analysis of the alternative splicing distribution in human duplicate pairs showed the asymmetric evolution of alternative splicing after gene duplications; i.e., the AS forms between duplicates may differ dramatically. We therefore conclude that alternative splicing and gene duplication may not evolve independently. In the early stage after gene duplication, young duplicates may take over a certain amount of protein function diversity that previously was carried out by the alternative splicing mechanism. In the late stage, the gain and loss of alternative splicing seem to be independent between duplicates. PMID:16365379

  9. Alternative Programs in Teacher Education.

    ERIC Educational Resources Information Center

    Ditosto, Evelyn, Ed.; Huddle, Eugene, Ed.

    Practices and patterns in teacher education were explored at this meeting. Speeches are reprinted in this report on the following subjects: (1) the nature of educational alternatives; (2) the need for quality education for children of every race; (3) training programs designed for a varied group of professionals within a community of…

  10. Academic Programs in Alternative Education: An Overview

    ERIC Educational Resources Information Center

    Ruzzi, Betsy Brown; Kraemer, Jacqueline

    2006-01-01

    This paper, second in a series of papers on alternative education, examines the academic programming in alternative education programs by reviewing the literature specifically focused on the academic programs in alternative education and summarizing a survey of fifteen alternative education programs. It suggests options for further research on…

  11. Alternative Programs for Disruptive Youth.

    ERIC Educational Resources Information Center

    Thomas, M. Angele, Ed.; And Others

    The book addresses issues in meeting the educational needs of disruptive students. In the introduction, R. Sarri examines the rise of alternative schools and discusses common elements in their design and operation. D. Sabatino follows with "Issues and Concerns: Problems with Alternative Schools," in which he examines the particular difficulties…

  12. ["Matreshka" Genes with Alternative Reading Frames].

    PubMed

    Sheshukova, E V; Shindyapina, A V; Komarova, T V; Dorokhov, Yu L

    2016-02-01

    Although a relatively small part of the human genome contains protein encoding genes, the latest data on the discovery of alternative open reading frames (ORFs) in conventional mRNAs has highlighted the expanded coding potential of these genes. Until recently, it was believed that each mRNA transcript encodes a single protein. Recent proteogenomics data indicate the existence of exceptions to this rule, which greatly changes the usual meaning of the term "gene". The topology of a gene with overlapping ORFs resembles a Russian "matreshka" toy. There are two levels of "matreshka" genetic systems. First, the chromosomal level, when the "nested" gene is located within introns and exons of the main chromosomal gene, both in the sense and antisense orientation relative to the external gene. The second level is a mature mRNA molecule containing overlapping ORFs or an ORF with an alternative-start codon. In this review we will focus on the properties of "matreshka" genes of the second type and methods for their detection and verification. Particular attention is paid to the biological properties of the polypeptides encoded by these genes. PMID:27215029

  13. Gene and alternative splicing annotation with AIR

    PubMed Central

    Florea, Liliana; Di Francesco, Valentina; Miller, Jason; Turner, Russell; Yao, Alison; Harris, Michael; Walenz, Brian; Mobarry, Clark; Merkulov, Gennady V.; Charlab, Rosane; Dew, Ian; Deng, Zuoming; Istrail, Sorin; Li, Peter; Sutton, Granger

    2005-01-01

    Designing effective and accurate tools for identifying the functional and structural elements in a genome remains at the frontier of genome annotation owing to incompleteness and inaccuracy of the data, limitations in the computational models, and shifting paradigms in genomics, such as alternative splicing. We present a methodology for the automated annotation of genes and their alternatively spliced mRNA transcripts based on existing cDNA and protein sequence evidence from the same species or projected from a related species using syntenic mapping information. At the core of the method is the splice graph, a compact representation of a gene, its exons, introns, and alternatively spliced isoforms. The putative transcripts are enumerated from the graph and assigned confidence scores based on the strength of sequence evidence, and a subset of the high-scoring candidates are selected and promoted into the annotation. The method is highly selective, eliminating the unlikely candidates while retaining 98% of the high-quality mRNA evidence in well-formed transcripts, and produces annotation that is measurably more accurate than some evidence-based gene sets. The process is fast, accurate, and fully automated, and combines the traditionally distinct gene annotation and alternative splicing detection processes in a comprehensive and systematic way, thus considerably aiding in the ensuing manual curation efforts. PMID:15632090

  14. Alternative Programs and Desegregation in Flint, Michigan

    ERIC Educational Resources Information Center

    Kugler, Marianne Russell

    1978-01-01

    Discusses the results of the Flint, Michigan Board of Education's decision to include extensive development of alternative programs as part of the Quality Education/Desegregation Plan developed for the Flint schools. (Author/RK)

  15. Program for Alternative Careers in Education (PACE).

    ERIC Educational Resources Information Center

    Flicker, Bernard

    The overall objective of the Program for Alternative Careers in Education (PACE) is to develop an alternative model of teacher preparation that prepares individuals to function as educators in the widest possible variety of educational institutions including public and private human service institutions, social welfare agencies, communication…

  16. Alternate nozzle ablative materials program

    NASA Technical Reports Server (NTRS)

    Kimmel, N. A.

    1984-01-01

    Four subscale solid rocket motor tests were conducted successfully to evaluate alternate nozzle liner, insulation, and exit cone structural overwrap components for possible application to the Space Shuttle Solid Rocket Motor (SRM) nozzle asasembly. The 10,000 lb propellant motor tests were simulated, as close as practical, the configuration and operational environment of the full scale SRM. Fifteen PAN based and three pitch based materials had no filler in the phenolic resin, four PAN based materials had carbon microballoons in the resin, and the rest of the materials had carbon powder in the resin. Three nozzle insulation materials were evaluated; an aluminum oxide silicon oxide ceramic fiber mat phenolic material with no resin filler and two E-glass fiber mat phenolic materials with no resin filler. It was concluded by MTI/WD (the fabricator and evaluator of the test nozzles) and NASA-MSFC that it was possible to design an alternate material full scale SRM nozzle assembly, which could provide an estimated 360 lb increased payload capability for Space Shuttle launches over that obtainable with the current qualified SRM design.

  17. Bridge Program: An Alternative Curricular Model

    ERIC Educational Resources Information Center

    George, Deborah A.

    2012-01-01

    With the motivation for career advancement, many adult learners have chosen to return to graduate education or professional programs. The bridge program is one relatively new alternative curricular model available for adult learners who wish to build on their education within their chosen profession. Evidence on the effectiveness of such programs…

  18. Alternatives for Revitalizing Student Services Programs.

    ERIC Educational Resources Information Center

    Deegan, William L.

    1984-01-01

    Reviews alternatives for revitalizing the programs and management of community college student services. As program development models, considers Miami-Dade Community College's computer-based instructional management system; entrepreneurial fee-based services; and divestment of situational or special-interest services to student groups. In…

  19. Alternative promoters of gene MAGE4a

    SciTech Connect

    De Plaen, E.; Naerhuyzen, B.; De Smet, C.

    1997-03-01

    Gene MAGE-4 (HGMW-approved symbol MAGE4) is expressed in several types of tumors, but not in normal tissues, except testis and placenta. The 5{prime} end of this gene contains eight homologous exons spread over a 5.8-kb region. These exons are alternatively spliced to a unique second exon and a unique third exon, which encodes a protein of 317 amino acids. The analysis of transcripts found in testis, placenta, and a sarcoma cell line showed that each of the alternative first exons is used in at least one of these tissues. Various regions of the promoter of the fifth alternative exon (1.5) were cloned in a luciferase reporter plasmid, and the constructs were transfected in a sarcoma cell line that expresses MAGE-4. Two Ets motifs located between positions -70 and -29 relative to the transcription start site were found to drive 55% of the promoter activity. A region containing an Sp1 consensus binding site located upstream of the two Ets motifs was found to be responsible for 44% of the transcriptional activity. MAGE-4a promoters 1.4 and 1.6, which also contain the Sp1 and the two Ets binding motifs, supported a level of transcription comparable to that of promoter 1.5, whereas promoter 1.1, which contains only one Ets binding site, was sixfold less active. In line with observations made with gene MAGE-1 (HGMW-approved symbol MAGE1), we found that promoter 1.5 stimulated a high level of transcription in a melanoma cell line that does not express MAGE-4. This suggests that the tumor-specific expression of MAGE genes is not determined by the presence of specific transcription factors. 26 refs., 7 figs., 2 tabs.

  20. 76 FR 11310 - Alternatives Analysis Program Discretionary Funding Allocations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-01

    ... Federal Transit Administration Alternatives Analysis Program Discretionary Funding Allocations AGENCY: Federal Transit Administration (FTA), DOT. ACTION: Alternatives Analysis Program Announcement of Project...) announces the selection of projects funded with unallocated Section 5339 Alternatives Analysis Program...

  1. Vials: Visualizing Alternative Splicing of Genes

    PubMed Central

    Strobelt, Hendrik; Alsallakh, Bilal; Botros, Joseph; Peterson, Brant; Borowsky, Mark; Pfister, Hanspeter; Lex, Alexander

    2016-01-01

    Alternative splicing is a process by which the same DNA sequence is used to assemble different proteins, called protein isoforms. Alternative splicing works by selectively omitting some of the coding regions (exons) typically associated with a gene. Detection of alternative splicing is difficult and uses a combination of advanced data acquisition methods and statistical inference. Knowledge about the abundance of isoforms is important for understanding both normal processes and diseases and to eventually improve treatment through targeted therapies. The data, however, is complex and current visualizations for isoforms are neither perceptually efficient nor scalable. To remedy this, we developed Vials, a novel visual analysis tool that enables analysts to explore the various datasets that scientists use to make judgments about isoforms: the abundance of reads associated with the coding regions of the gene, evidence for junctions, i.e., edges connecting the coding regions, and predictions of isoform frequencies. Vials is scalable as it allows for the simultaneous analysis of many samples in multiple groups. Our tool thus enables experts to (a) identify patterns of isoform abundance in groups of samples and (b) evaluate the quality of the data. We demonstrate the value of our tool in case studies using publicly available datasets. PMID:26529712

  2. Alternative-splicing-mediated gene expression

    NASA Astrophysics Data System (ADS)

    Wang, Qianliang; Zhou, Tianshou

    2014-01-01

    Alternative splicing (AS) is a fundamental process during gene expression and has been found to be ubiquitous in eukaryotes. However, how AS impacts gene expression levels both quantitatively and qualitatively remains to be fully explored. Here, we analyze two common models of gene expression, each incorporating a simple splice mechanism that a pre-mRNA is spliced into two mature mRNA isoforms in a probabilistic manner. In the constitutive expression case, we show that the steady-state molecular numbers of two mature mRNA isoforms follow mutually independent Poisson distributions. In the bursting expression case, we demonstrate that the tail decay of the steady-state distribution for both mature mRNA isoforms that in general are not mutually independent can be characterized by the product of mean burst size and splicing probability. In both cases, we find that AS can efficiently modulate both the variability (measured by variance) and the noise level of the total mature mRNA, and in particular, the latter is always lower than the noise level of the pre-mRNA, implying that AS always reduces the noise. These results altogether reveal that AS is a mechanism of efficiently controlling the gene expression noise.

  3. School Counseling in Disciplinary Alternative Education Programs

    ERIC Educational Resources Information Center

    Mullen, Patrick R.; Lambie, Glenn W.

    2013-01-01

    Disciplinary alternative education programs (DAEP) service many students; however, limited literature is published for school counselors working in these schools. Therefore, this manuscript provides a conceptual foundation for counselors working with students attending DAEPs. Specifically, the manuscript (a) reviews the types of alternative…

  4. Alternative Training Using Selected Program Planning Concepts.

    ERIC Educational Resources Information Center

    Zappala, John

    1996-01-01

    Discusses a study to determine the extent that Michigan Adult Education Alternative Training (AEAT) providers used selected concepts of systematic planning in developing training programs. Finds that 55.6% of 65 responding AEAT planners utilized the concepts. Suggests that planners should also incorporate political and practical considerations.…

  5. Indiana University Alternative School Teacher Education Program.

    ERIC Educational Resources Information Center

    Indiana Univ., Bloomington. School of Education.

    As public school districts have begun to develop optional alternative schools, a common concern has been where to find trained teachers to staff the schools. For the past four years Indiana University has worked closely with several of these school districts in developing a field-based teacher education program to prepare new teachers for these…

  6. Dormancy programs as emerging antimetastasis therapeutic alternatives

    PubMed Central

    Sosa, Maria Soledad

    2016-01-01

    We recently published that the retinoid-responsive gene NR2F1 (nuclear receptor subfamily 2, group F, member 1) mediates postsurgical dormancy of local residual tumor cells and disseminated tumor cells. Importantly, the combination of azacytidine with retinoids induces dormancy of malignant tumor cells by reinstating the NR2F1-regulated gene program. These findings open the door to the development of strategies that may stop minimal residual disease from becoming life-threatening metastases. PMID:27308542

  7. Alternative Learning Programs Evaluation: Part 3 Report. Case Studies of Alternative Schools and Programs.

    ERIC Educational Resources Information Center

    Cobb, Caronly; Brewer, Delores; Bauman, Amy; Groves, Paula; Rayle, Joesph; Noblit, George

    In 1996, North Carolina began a 5-year evaluation of alternative learning programs (ALPs). This report contains in-depth case studies of four ALPs during the 1996-97 school year. The ALPs were selected to represent different types of programs in the state, and include one middle school, two high school, and one elementary school programs. The case…

  8. An alternative method on quadratic programming problems

    NASA Astrophysics Data System (ADS)

    Dasril, Y.; Mohd, I. B.; Mustaffa, I.; Aminuddin, MMM.

    2015-05-01

    In this paper we proposed an alternative approach to find the optimum solution of quadratic programming problems (QPP) in its original form without additional information such as slack variable, surplus variable or artificial variable as done in other favourite methods. This approached is based on the violated constraints by the unconstrained optimum. The optimal solution of QPP obtained by searching from initial point to another point alongside of feasible region.

  9. 45 CFR 400.69 - Alternative RCA programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false Alternative RCA programs. 400.69 Section 400.69... Assistance § 400.69 Alternative RCA programs. A State that determines that a public/private RCA program or a... choose instead to establish an alternative approach under the Wilson/Fish program, authorized by...

  10. 45 CFR 400.69 - Alternative RCA programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Alternative RCA programs. 400.69 Section 400.69... Assistance § 400.69 Alternative RCA programs. A State that determines that a public/private RCA program or a... choose instead to establish an alternative approach under the Wilson/Fish program, authorized by...

  11. 45 CFR 400.69 - Alternative RCA programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Alternative RCA programs. 400.69 Section 400.69... Assistance § 400.69 Alternative RCA programs. A State that determines that a public/private RCA program or a... choose instead to establish an alternative approach under the Wilson/Fish program, authorized by...

  12. Association of American Railroads Alternative Fuels Program

    SciTech Connect

    Furber, C.P.

    1985-01-01

    Alternative fuels can be used in locomotive diesel engines as a means to reduce fuel costs or as fuel extenders when sufficient quantities of suitable lower cost fuels are not available. Broadened fuel purchasing guidelines, based on engine fuel tolerance limitation tests, offer a potential for reducing fuel costs. Fuels such as alcohols, certain vegetable oils, shale oils, and heavy oil blends can be used to extend fuel supplies. Fuel tolerance limitations of existing engines can be increased through modifications such as staged injection or the use of ceramic coatings. This paper describes the methods used by the Association of American Railroads Alternative Fuels Research Program to determine engine fuel tolerance limitations and extend engine fuel tolerance limits.

  13. 36 CFR 800.14 - Federal agency program alternatives.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... alternatives. 800.14 Section 800.14 Parks, Forests, and Public Property ADVISORY COUNCIL ON HISTORIC PRESERVATION PROTECTION OF HISTORIC PROPERTIES Program Alternatives § 800.14 Federal agency program alternatives. (a) Alternate procedures. An agency official may develop procedures to implement section 106...

  14. 36 CFR 800.14 - Federal agency program alternatives.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... alternatives. 800.14 Section 800.14 Parks, Forests, and Public Property ADVISORY COUNCIL ON HISTORIC PRESERVATION PROTECTION OF HISTORIC PROPERTIES Program Alternatives § 800.14 Federal agency program alternatives. (a) Alternate procedures. An agency official may develop procedures to implement section 106...

  15. 36 CFR 800.14 - Federal agency program alternatives.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... alternatives. 800.14 Section 800.14 Parks, Forests, and Public Property ADVISORY COUNCIL ON HISTORIC PRESERVATION PROTECTION OF HISTORIC PROPERTIES Program Alternatives § 800.14 Federal agency program alternatives. (a) Alternate procedures. An agency official may develop procedures to implement section 106...

  16. 36 CFR 800.14 - Federal agency program alternatives.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... alternatives. 800.14 Section 800.14 Parks, Forests, and Public Property ADVISORY COUNCIL ON HISTORIC PRESERVATION PROTECTION OF HISTORIC PROPERTIES Program Alternatives § 800.14 Federal agency program alternatives. (a) Alternate procedures. An agency official may develop procedures to implement section 106...

  17. 33 CFR 104.140 - Alternative Security Programs.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Alternative Security Programs... MARITIME SECURITY MARITIME SECURITY: VESSELS General § 104.140 Alternative Security Programs. A vessel owner or operator may use an Alternative Security Program as approved under § 101.120 of this...

  18. 33 CFR 105.140 - Alternative Security Program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Alternative Security Program. 105... MARITIME SECURITY MARITIME SECURITY: FACILITIES General § 105.140 Alternative Security Program. (a) A facility owner or operator may use an Alternative Security Program approved under § 101.120 of...

  19. 33 CFR 104.140 - Alternative Security Programs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Alternative Security Programs... MARITIME SECURITY MARITIME SECURITY: VESSELS General § 104.140 Alternative Security Programs. A vessel owner or operator may use an Alternative Security Program as approved under § 101.120 of this...

  20. 33 CFR 104.140 - Alternative Security Programs.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Alternative Security Programs... MARITIME SECURITY MARITIME SECURITY: VESSELS General § 104.140 Alternative Security Programs. A vessel owner or operator may use an Alternative Security Program as approved under § 101.120 of this...

  1. 33 CFR 105.140 - Alternative Security Program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Alternative Security Program. 105... MARITIME SECURITY MARITIME SECURITY: FACILITIES General § 105.140 Alternative Security Program. (a) A facility owner or operator may use an Alternative Security Program approved under § 101.120 of...

  2. 33 CFR 106.135 - Alternative Security Program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Alternative Security Program. 106... MARITIME SECURITY MARINE SECURITY: OUTER CONTINENTAL SHELF (OCS) FACILITIES General § 106.135 Alternative Security Program. An OCS facility owner or operator may use an Alternative Security Program approved...

  3. 33 CFR 105.140 - Alternative Security Program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Alternative Security Program. 105... MARITIME SECURITY MARITIME SECURITY: FACILITIES General § 105.140 Alternative Security Program. (a) A facility owner or operator may use an Alternative Security Program approved under § 101.120 of...

  4. Alternative Gene Form Discovery and Candidate Gene Selection from Gene Indexing Projects

    PubMed Central

    Burke, John; Wang, Hui; Hide, Winston; Davison, Daniel B.

    1998-01-01

    Several efforts are under way to partition single-read expressed sequence tag (EST), as well as full-length transcript data, into large-scale gene indices, where transcripts are in common index classes if and only if they share a common progenitor gene. Accurate gene indexing facilitates gene expression studies, as well as inexpensive and early gene sequence discovery through assembly of ESTs that are derived from genes that have not been sequenced by classical methods. We extend, correct, and enhance the information obtained from index groups by splitting index classes into subclasses based on sequence dissimilarity (diversity). Two applications of this are highlighted in this report. First it is shown that our method can ameliorate the damage that artifacts, such as chimerism, inflict on index integrity. Additionally, we demonstrate how the organization imposed by an effective subpartition can greatly increase the sensitivity of gene expression studies by accounting for the existence and tissue- or pathology-specific regulation of novel gene isoforms and polymorphisms. We apply our subpartitioning treatment to the UniGene gene indexing project to measure a marked increase in information quality and abundance (in terms of assembly length and insertion/deletion error) after treatment and demonstrate cases where new levels of information concerning differential expression of alternate gene forms, such as regulated alternative splicing, are discovered. [Tables 2 and 3 can be viewed in their entirety as Online Supplements at http://www.genome.org.] PMID:9521931

  5. Alternative Education Guidelines, Model Middle School Alternative Education Program. Workshop II. Prevention Programs & Funding.

    ERIC Educational Resources Information Center

    National Association of School Security Directors, Bellevue, WA.

    These workshop materials are the guidelines Broward County (Florida) has established to carry out the state legislative mandate authorizing school boards to establish educational alternative programs to meet the individual needs and interests of students who are disruptive, unsucessful, or disinterested in the normal school environment. Included…

  6. An Analysis of State Policies Connected with Alternative School Programs

    ERIC Educational Resources Information Center

    Bielefeld, William C.; Stubblefield, Anna; Templeton, Zach

    2009-01-01

    Many school districts offer alternative education programs for their students who do not succeed in the regular classroom. At the high school level, many of these programs are separated from the regular schools. It seemed that many districts were establishing alternative education programs, but there were differences in the types of programs that…

  7. Current and Needed Research on Alternative Certification Programs

    ERIC Educational Resources Information Center

    Ham, Edward

    2010-01-01

    With alternative certification programs gaining popularity in teacher education, the need to evaluate these programs has become much more necessary. Without strict guidelines to classify alternative certification programs, it is difficult to make generalizations about these programs because of different requirements for completion and…

  8. Alternative Educational Programs for Gifted Students in Mathematics.

    ERIC Educational Resources Information Center

    House, Peggy A.

    1983-01-01

    Some alternatives available for meeting the needs of the mathematically gifted student at the secondary school level are examined. Along with curriculum modification are discussed such approaches as magnet programs, special schools, special classes, summer programs, and mentor programs. (MNS)

  9. Fourth annual report to Congress, Federal Alternative Motor Fuels Programs

    SciTech Connect

    1995-07-01

    This annual report to Congress presents the current status of the alternative fuel vehicle programs being conducted across the country in accordance with the Alternative Motor Fuels Act of 1988. These programs, which represent the most comprehensive data collection effort ever undertaken on alternative fuels, are beginning their fifth year. This report summarizes tests and results from the fourth year.

  10. Evaluating Organizational Training Programs: Alternatives and Criteria for Selection.

    ERIC Educational Resources Information Center

    Marth, Joseph; And Others

    Managers and program sponsors are often unaware of possible alternatives to costly training evaluation procedures and do not have criteria for selecting alternatives. What is needed is an understanding of the various levels of evaluating training programs, feasible alternatives, and decision criteria for choosing the right system. It is proposed…

  11. The Role of Alternative Programming in College Radio.

    ERIC Educational Resources Information Center

    Sauls, Samuel J.

    College radio is quite often viewed as the true alternative to commercial radio. However, what is alternative radio and how does college radio factor into the ideal? To further understand this concept, this paper focuses on the role of alternative programming in college radio. Areas discussed include alternative radio as a non-mainstream form of…

  12. EPAct Alternative Fuel Transporation Program - State and Alternative Fuel Provider Fleets: Frequently Asked Questions

    SciTech Connect

    2010-03-01

    Factsheet answering frequently asked questions about the U.S. Department of Energy's Alternative Fuel Transportation Program (the Program) that implements provisions of Titles III–V of the Energy Policy Act of 1992 (EPAct). Answers to questions that are frequently asked about the Program by managers of state government and alternative fuel provider fleets are provided in the factsheet.

  13. 7 CFR 58.141 - Alternate quality control program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Alternate quality control program. 58.141 Section 58... Service 1 Quality Specifications for Raw Milk § 58.141 Alternate quality control program. When a plant has in operation an acceptable quality program, at the producer level, which is approved by...

  14. 6 CFR 27.235 - Alternative security program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Alternative security program. 27.235 Section 27.235 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY CHEMICAL FACILITY ANTI-TERRORISM STANDARDS Chemical Facility Security Program § 27.235 Alternative security program. (a)...

  15. 45 CFR 400.69 - Alternative RCA programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Alternative RCA programs. 400.69 Section 400.69 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR... Assistance § 400.69 Alternative RCA programs. A State that determines that a public/private RCA program or...

  16. 7 CFR 58.141 - Alternate quality control program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Alternate quality control program. 58.141 Section 58... Service 1 Quality Specifications for Raw Milk § 58.141 Alternate quality control program. When a plant has in operation an acceptable quality program, at the producer level, which is approved by...

  17. Cloning and expression of the potato alternative oxidase gene

    SciTech Connect

    Hiser, C.; McIntosh, L. Michigan State Univ., East Lansing )

    1990-05-01

    Mitochondria from 24-hour-aged potato slices possess an alternative path capacity and a 36kD protein not present in fresh potato mitochondria. This 36kD protein was identified by a monoclonal antibody against the Sauromatum guttatum alternative oxidase. These results suggest de novo synthesis of the 36kD protein during the aging process. To investigate this phenomenon, a clone containing a potato alternative oxidase gene was isolated from a cDNA library using the S. guttatum gene as a probe. This clone shows areas of high homology to the S. guttatum gene. Norther blots of RNA from fresh and 24-hour-aged potato slices are being probed with the potato gene to examine its expression in relation to the appearance of the 36kD protein.

  18. An Alternative Program for Pregnant Schoolgirls.

    ERIC Educational Resources Information Center

    Link, Patricia W.

    The paper describes the Lafayette Parish Homebound Program (Louisiana) for students in grades K-12, with particular emphasis on the program's services for pregnant girls. Procedures for admitting students into the program, objectives for the pregnant girls (12-18 years old), and program components are considered. It is explained that the special…

  19. 24 CFR 7.5 - EEO Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Provisions § 7.5 EEO Alternative Dispute Resolution Program. In accordance with the Secretary's Policy Statement regarding Alternative Dispute Resolution (ADR) located on the Department's website and 29 CFR 1614... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false EEO Alternative Dispute...

  20. 33 CFR 101.125 - Approved Alternative Security Programs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Approved Alternative Security... SECURITY MARITIME SECURITY MARITIME SECURITY: GENERAL General § 101.125 Approved Alternative Security Programs. The following have been approved, by the Commandant (CG-54), as Alternative Security...

  1. 78 FR 31535 - Assistive Technology Alternative Financing Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ... On August 8, 2012, we published a notice in the Federal Register (77 FR 47375) inviting applications... Assistive Technology Alternative Financing Program AGENCY: Office of Special Education and Rehabilitative... Technology (AT) Alternative Financing Program (AFP) in fiscal year (FY) 2012 to make new grant awards in...

  2. Toward a New Era: Alternatives for Revitalizing Student Services Programs.

    ERIC Educational Resources Information Center

    Deegan, William L.

    Alternatives for revitalizing the programs and management of student services in community colleges are reviewed in this paper. First, alternatives related to student services programs are considered, including: (1) the increased use of computer-assisted counseling to integrate student services more fully with mainstream academic activities; (2)…

  3. Alternative Schools and Programs: Five Descriptions. Technical Assistance Bulletin 37.

    ERIC Educational Resources Information Center

    National School Resource Network, Washington, DC.

    During the past ten years, many school districts have established alternative schools or programs for students who are seriously disruptive and academically unsuccessful. This bulletin describes five different types of alternative programs that have been effective in dealing with disruptive youth. They include the Tri-C Community Centered…

  4. A serine–arginine-rich (SR) splicing factor modulates alternative splicing of over a thousand genes in Toxoplasma gondii

    PubMed Central

    Yeoh, Lee M.; Goodman, Christopher D.; Hall, Nathan E.; van Dooren, Giel G.; McFadden, Geoffrey I.; Ralph, Stuart A.

    2015-01-01

    Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoured in this phylum. We investigated the mechanism of alternative splicing in Toxoplasma gondii, and have identified and localized TgSR3, a homologue of ASF/SF2 (alternative-splicing factor/splicing factor 2, a serine-arginine–rich, or SR protein) to a subnuclear compartment. In addition, we conditionally overexpressed this protein, which was deleterious to growth. qRT-PCR was used to confirm perturbation of splicing in a known alternatively-spliced gene. We performed high-throughput RNA-seq to determine the extent of splicing modulated by this protein. Current RNA-seq algorithms are poorly suited to compact parasite genomes, and hence we complemented existing tools by writing a new program, GeneGuillotine, that addresses this deficiency by segregating overlapping reads into distinct genes. In order to identify the extent of alternative splicing, we released another program, JunctionJuror, that detects changes in intron junctions. Using this program, we identified about 2000 genes that were constitutively alternatively spliced in T. gondii. Overexpressing the splice regulator TgSR3 perturbed alternative splicing in over 1000 genes. PMID:25870410

  5. A serine-arginine-rich (SR) splicing factor modulates alternative splicing of over a thousand genes in Toxoplasma gondii.

    PubMed

    Yeoh, Lee M; Goodman, Christopher D; Hall, Nathan E; van Dooren, Giel G; McFadden, Geoffrey I; Ralph, Stuart A

    2015-05-19

    Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoured in this phylum. We investigated the mechanism of alternative splicing in Toxoplasma gondii, and have identified and localized TgSR3, a homologue of ASF/SF2 (alternative-splicing factor/splicing factor 2, a serine-arginine-rich, or SR protein) to a subnuclear compartment. In addition, we conditionally overexpressed this protein, which was deleterious to growth. qRT-PCR was used to confirm perturbation of splicing in a known alternatively-spliced gene. We performed high-throughput RNA-seq to determine the extent of splicing modulated by this protein. Current RNA-seq algorithms are poorly suited to compact parasite genomes, and hence we complemented existing tools by writing a new program, GeneGuillotine, that addresses this deficiency by segregating overlapping reads into distinct genes. In order to identify the extent of alternative splicing, we released another program, JunctionJuror, that detects changes in intron junctions. Using this program, we identified about 2000 genes that were constitutively alternatively spliced in T. gondii. Overexpressing the splice regulator TgSR3 perturbed alternative splicing in over 1000 genes. PMID:25870410

  6. Choosing among Alternative Programs for Poor Children.

    ERIC Educational Resources Information Center

    Currie, Janet M.

    1997-01-01

    Suggests four criteria (efficiency, return on investment, incentives, and equity) for evaluating and comparing public programs for poor children, and provides an overview of information available on eight large federal programs using these criteria. Positive effects of some programs are noted, and policy recommendations the evidence supports are…

  7. THE ALTERNATIVE COVERS ASSESSMENT PROGRAM (ACAP)

    EPA Science Inventory

    Alternative covers attempt to achieve equivalent performance to conventional impermeable covers through an action that has been described as 'sponge and pump'. In this type of cover system, the soil and plants absorb moisture from precipitation, store it in the plant and soil str...

  8. John Rennie High School Alternative Learning Program.

    ERIC Educational Resources Information Center

    Robertson, Donald R.

    The paper charts the development of a gifted program for secondary students in Pointe Claire, Quebec. The program featured an unstructured learning environment maximizing student choices, a resource teacher and facility, and learning experiences outside of school. Selection criteria are explained and a sample behavior rating scale is appended.…

  9. Abnormalities in Alternative Splicing of Apoptotic Genes and Cardiovascular Diseases

    PubMed Central

    Dlamini, Zodwa; Tshidino, Shonisani C.; Hull, Rodney

    2015-01-01

    Apoptosis is required for normal heart development in the embryo, but has also been shown to be an important factor in the occurrence of heart disease. Alternative splicing of apoptotic genes is currently emerging as a diagnostic and therapeutic target for heart disease. This review addresses the involvement of abnormalities in alternative splicing of apoptotic genes in cardiac disorders including cardiomyopathy, myocardial ischemia and heart failure. Many pro-apoptotic members of the Bcl-2 family have alternatively spliced isoforms that lack important active domains. These isoforms can play a negative regulatory role by binding to and inhibiting the pro-apoptotic forms. Alternative splicing is observed to be increased in various cardiovascular diseases with the level of alternate transcripts increasing elevated in diseased hearts compared to healthy subjects. In many cases these isoforms appear to be the underlying cause of the disease, while in others they may be induced in response to cardiovascular pathologies. Regardless of this, the detection of alternate splicing events in the heart can serve as useful diagnostic or prognostic tools, while those splicing events that seem to play a causative role in cardiovascular disease make attractive future drug targets. PMID:26580598

  10. Alternative splicing of inner-ear-expressed genes.

    PubMed

    Wang, Yanfei; Liu, Yueyue; Nie, Hongyun; Ma, Xin; Xu, Zhigang

    2016-09-01

    Alternative splicing plays a fundamental role in the development and physiological function of the inner ear. Inner-ear-specific gene splicing is necessary to establish the identity and maintain the function of the inner ear. For example, exon 68 of Cadherin 23 (Cdh23) gene is subject to inner-ear-specific alternative splicing, and as a result, Cdh23(+ 68) is only expressed in inner ear hair cells. Alternative splicing along the tonotopic axis of the cochlea contributes to frequency tuning, particularly in lower vertebrates, such as chickens and turtles. Differential splicing of Kcnma1, which encodes for the α subunit of the Ca(2+)-activated K(+) channel (BK channel), has been suggested to affect the channel gating properties and is important for frequency tuning. Consequently, deficits in alternative splicing have been shown to cause hearing loss, as we can observe in Bronx Waltzer (bv) mice and Sfswap mutant mice. Despite the advances in this field, the regulation of alternative splicing in the inner ear remains elusive. Further investigation is also needed to clarify the mechanism of hearing loss caused by alternative splicing deficits. PMID:27376950

  11. Alternative Strategies for Funding a General Dentistry Residency Program.

    ERIC Educational Resources Information Center

    Kralewski, John E.; Wiggins, Carla

    1987-01-01

    Three alternative program funding approaches used in other professions are examined: (1) the reorientation of selected dental schools toward graduate education, (2) emphasizing and marketing the service aspects of the programs, and (3) developing education programs as in-house training for large organizations. (MSE)

  12. Parent Education: Key to Successful Alternative Education Programs.

    ERIC Educational Resources Information Center

    Buroker, Charles D.

    1993-01-01

    Examines the relationship between parental involvement and alternative education programs and reports results of a highly successful parent education program (Active Parenting) in Lima, Ohio. This video-based discussion program evinced high participant satisfaction, specifically encouraged 15 positive parenting behaviors, and discouraged some…

  13. Spaces of Difference: The Contradictions of Alternative Educational Programs

    ERIC Educational Resources Information Center

    Vadeboncoeur, Jennifer A.

    2009-01-01

    Drawing upon the concept of "thirdspace" (Soja 1996), this article extends sociocultural theorizations of space in relation to alternative educational programs: programs designed to re-engage youth who have been pushed out of mainstream schools. Snapshots of educational programs, provided by ethnographic research gathered in the United States,…

  14. Alternative mathematical programming formulations for FSS synthesis

    NASA Technical Reports Server (NTRS)

    Reilly, C. H.; Mount-Campbell, C. A.; Gonsalvez, D. J. A.; Levis, C. A.

    1986-01-01

    A variety of mathematical programming models and two solution strategies are suggested for the problem of allocating orbital positions to (synthesizing) satellites in the Fixed Satellite Service. Mixed integer programming and almost linear programming formulations are presented in detail for each of two objectives: (1) positioning satellites as closely as possible to specified desired locations, and (2) minimizing the total length of the geostationary arc allocated to the satellites whose positions are to be determined. Computational results for mixed integer and almost linear programming models, with the objective of positioning satellites as closely as possible to their desired locations, are reported for three six-administration test problems and a thirteen-administration test problem.

  15. Molecular trajectories leading to the alternative fates of duplicate genes.

    PubMed

    Marotta, Michael; Piontkivska, Helen; Tanaka, Hisashi

    2012-01-01

    Gene duplication generates extra gene copies in which mutations can accumulate without risking the function of pre-existing genes. Such mutations modify duplicates and contribute to evolutionary novelties. However, the vast majority of duplicates appear to be short-lived and experience duplicate silencing within a few million years. Little is known about the molecular mechanisms leading to these alternative fates. Here we delineate differing molecular trajectories of a relatively recent duplication event between humans and chimpanzees by investigating molecular properties of a single duplicate: DNA sequences, gene expression and promoter activities. The inverted duplication of the Glutathione S-transferase Theta 2 (GSTT2) gene had occurred at least 7 million years ago in the common ancestor of African great apes and is preserved in chimpanzees (Pan troglodytes), whereas a deletion polymorphism is prevalent in humans. The alternative fates are associated with expression divergence between these species, and reduced expression in humans is regulated by silencing mutations that have been propagated between duplicates by gene conversion. In contrast, selective constraint preserved duplicate divergence in chimpanzees. The difference in evolutionary processes left a unique DNA footprint in which dying duplicates are significantly more similar to each other (99.4%) than preserved ones. Such molecular trajectories could provide insights for the mechanisms underlying duplicate life and death in extant genomes. PMID:22720000

  16. 24 CFR 7.26 - EEO Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false EEO Alternative Dispute Resolution...-Complaint Processing § 7.26 EEO Alternative Dispute Resolution Program. (a) The aggrieved person may elect... chosen, the EEO Counselor shall advise the aggrieved person that if the dispute is resolved during...

  17. 24 CFR 7.26 - EEO Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false EEO Alternative Dispute Resolution...-Complaint Processing § 7.26 EEO Alternative Dispute Resolution Program. (a) The aggrieved person may elect... chosen, the EEO Counselor shall advise the aggrieved person that if the dispute is resolved during...

  18. 76 FR 55136 - Implementation of the Alternative Dispute Resolution Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-06

    ... COMMISSION Implementation of the Alternative Dispute Resolution Program AGENCY: Nuclear Regulatory Commission... Administrative Dispute Resolution Act (Act) which requires each Federal agency to, among other things; adopt a... 2011 or early November 2011 to solicit feedback from its stakeholders on its Alternative...

  19. Evaluating Alternative High Schools: Program Evaluation in Action

    ERIC Educational Resources Information Center

    Hinds, Drew Samuel Wayne

    2013-01-01

    Alternative high schools serve some of the most vulnerable students and their programs present a significant challenge to evaluate. Determining the impact of an alternative high school that serves mostly at-risk students presented a significant research problem. Few studies exist that dig deeper into the characteristics and strategies of…

  20. Quality Alternative Certification Programs in Special Education Ensure High Retention

    ERIC Educational Resources Information Center

    Karge, Belinda D.; McCabe, Marjorie

    2014-01-01

    Market driven alternative routes to teaching have evolved into a quality program option and not just an answer to the teacher shortage. Alternative certification is a viable means of recruiting, training, and certifying those who have a bachelor's degree and a strong desire to enter the field of teaching. California has been a leader in the…

  1. 33 CFR 155.5061 - Alternative Training and Exercise Program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... how the Alternative Training and Exercise Program addresses the requirements of 33 CFR 155.1055(b) through (f) and 33 CFR 155.1060; and (3) An explanation of how vessel owners or operators must implement... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Alternative Training and...

  2. Evaluation of the Union County Alternative to Suspension Program

    ERIC Educational Resources Information Center

    Dunlap, Joyce Ann

    2010-01-01

    The schools in Union County have undergone a tremendous amount of growth in the past decade. The growth in the county has led to an increase in discipline problems. In order to provide suspended students a second chance, Union County Public Schools implemented an alternative to suspension program, the Union County Alternative to Suspension Program…

  3. EPAct Alternative Fuel Transportation Program: Success Story (Fact Sheet)

    SciTech Connect

    Not Available

    2010-08-01

    This success story highlights the EPAct Alternative Fuel Transportation Program's series of workshops that bring fleets regulated under the Energy Policy Act of 1992 (EPAct) together with Clean Cities stakeholders and fuel providers to form and strengthen regional partnerships and initiate projects that will deploy more alternative fuel infrastructure.

  4. Another Alternative: A Ninety-Day Contractual Detoxification Treatment Program

    ERIC Educational Resources Information Center

    Kahn, Robert B.; And Others

    1976-01-01

    In May, 1974, Fresno County's Narcotic Abuse Treatment Program began a twenty-one-day outpatient methadone detoxification treatment modality. The results of the evaluation suggested an alternative treatment modality. The purpose of this paper is to examine this alternative treatment modality, its characteristics, its therapeutic outcomes and the…

  5. Conservation of alternative polyadenylation patterns in mammalian genes

    PubMed Central

    Ara, Takeshi; Lopez, Fabrice; Ritchie, William; Benech, Philippe; Gautheret, Daniel

    2006-01-01

    Background Alternative polyadenylation is a widespread mechanism contributing to transcript diversity in eukaryotes. Over half of mammalian genes are alternatively polyadenylated. Our understanding of poly(A) site evolution is limited by the lack of a reliable identification of conserved, equivalent poly(A) sites among species. We introduce here a working definition of conserved poly(A) sites as sites that are both (i) properly aligned in human and mouse orthologous 3' untranslated regions (UTRs) and (ii) supported by EST or cDNA data in both species. Results We identified about 4800 such conserved poly(A) sites covering one third of the orthologous gene set studied. Characteristics of conserved poly(A) sites such as processing efficiency and tissue-specificity were analyzed. Conserved sites show a higher processing efficiency but no difference in tissular distribution when compared to non-conserved sites. In general, alternative poly(A) sites are species-specific and involve minor, non-conserved sites that are unlikely to play essential roles. However, there are about 500 genes with conserved tandem poly(A) sites. A significant fraction of these conserved tandems display a conserved arrangement of major/minor sites in their 3' UTR, suggesting that these alternative 3' ends may be under selection. Conclusion This analysis allows us to identify potential functional alternative poly(A) sites and provides clues on the selective mechanisms at play in the appearance of multiple poly(A) sites and their maintenance in the 3' UTRs of genes. PMID:16872498

  6. Alternatives for Financing School Energy Savings Programs.

    ERIC Educational Resources Information Center

    Esteves, Rich

    1983-01-01

    This report compares shared-savings programs with financing through the use of internal funds, loans, leases, and lease purchase plans for financing energy conservation in nonprofit buildings. The shared savings option was found to offer the greatest benefits to the customer. (MLF)

  7. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.

    PubMed

    Simpson, Craig G; Fuller, John; Calixto, Cristiane P G; McNicol, Jim; Booth, Clare; Brown, John W S; Staiger, Dorothee

    2016-01-01

    Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments. PMID:26867620

  8. Fifth annual report to congress. Federal alternative motor fuels programs

    SciTech Connect

    1996-09-01

    This report presents the status of the US Department of Energy`s alternative fuel vehicle demonstration and performance tracking programs being conducted in accordance with the Energy Policy and Conservation Act. These programs comprise the most comprehensive data collection effort ever undertaken on alternative transportation fuels and alternative fuel vehicles. The report summarizes tests and results from the fifth year. Electric vehicles are not included in these programs, and the annual report does not include information on them. Since the inception of the programs, great strides have been made in developing commercially viable alternative fuel vehicle technologies. However, as is the case in the commercialization of all new technologies, some performance problems have been experienced on vehicles involved in early demonstration efforts. Substantial improvements have been recorded in vehicle practicality, safety, and performance in real-world demonstrations. An aspect of particular interest is emissions output. Results from light duty alternative fuel vehicles have demonstrated superior inservice emissions performance. Heavy duty alternative fuel vehicles have demonstrated dramatic reductions in particulate emissions. However, emissions results from vehicles converted to run on alternative fuel have not been as promising. Although the technologies available today are commercially viable in some markets, further improvements in infrastructure and economics will result in greater market expansion. Information is included in this report on light and heavy duty vehicles, transit buses, vehicle conversions, safety, infrastructure support, vehicle availability, and information dissemination.

  9. Alternatives. Alternative Discipline and Suspension Program Handbook, Campbell County Junior High.

    ERIC Educational Resources Information Center

    Campbell County School District 1, Gillette, WY.

    This booklet explains a program of discipline and suspension for students who come in conflict with school policy. The Alternative Discipline and Suspension Program (ADSP) operates in an environment of strict adherence to set rules where a student must earn advancement through and eventually out of ADSP back to regular classroom attendance. This…

  10. Hawaii alternative fuels utilization program. Phase 3, final report

    SciTech Connect

    Kinoshita, C.M.; Staackmann, M.

    1996-08-01

    The Hawaii Alternative Fuels Utilization Program originated as a five-year grant awarded by the US Department of Energy (USDOE) to the Hawaii Natural Energy Institute (HNEI) of the University of Hawaii at Manoa. The overall program included research and demonstration efforts aimed at encouraging and sustaining the use of alternative (i.e., substitutes for gasoline and diesel) ground transportation fuels in Hawaii. Originally, research aimed at overcoming technical impediments to the widespread adoption of alternative fuels was an important facet of this program. Demonstration activities centered on the use of methanol-based fuels in alternative fuel vehicles (AFVs). In the present phase, operations were expanded to include flexible fuel vehicles (FFVs) which can operate on M85 or regular unleaded gasoline or any combination of these two fuels. Additional demonstration work was accomplished in attempting to involve other elements of Hawaii in the promotion and use of alcohol fuels for ground transportation in Hawaii.

  11. Selectable marker genes in transgenic plants: applications, alternatives and biosafety.

    PubMed

    Miki, Brian; McHugh, Sylvia

    2004-02-01

    used. Many of the genes have specific limitations or have not been sufficiently tested to merit their widespread use. For research, a variety of selection systems are essential as no single selectable marker gene was found to be sufficient for all circumstances. Although, no adverse biosafety effects have been reported for the marker genes that have been adopted for widespread use, biosafety concerns should help direct which markers will be chosen for future crop development. Common sense dictates that marker genes conferring resistance to significant therapeutic antibiotics should not be used. An area of research that is growing rapidly but is still in its infancy is the development of strategies for eliminating selectable marker genes to generate marker-free plants. Among the several technologies described, two have emerged with significant potential. The simplest is the co-transformation of genes of interest with selectable marker genes followed by the segregation of the separate genes through conventional genetics. The more complicated strategy is the use of site-specific recombinases, under the control of inducible promoters, to excise the marker genes and excision machinery from the transgenic plant after selection has been achieved. In this review each of the genes and processes will be examined to assess the alternatives that exist for producing transgenic plants. PMID:14736458

  12. An Evaluative Study of Texas Alternative Certification Programs.

    ERIC Educational Resources Information Center

    Wale, William M.; Irons, E. Jane

    Results of an evaluative study of the Texas Alternative Certification Program (ACP), conducted by the Texas Educational Agency staff during the 1988-89 school year, are reported. The first ACP was implemented in a single urban school district during the 1985-86 school year. The program was designed, in part, to address the shortage of teachers. By…

  13. 24 CFR 7.26 - EEO Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false EEO Alternative Dispute Resolution Program. 7.26 Section 7.26 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development EQUAL EMPLOYMENT OPPORTUNITY; POLICY, PROCEDURES AND PROGRAMS Equal Employment Opportunity Without Regard to Race,...

  14. 45 CFR 400.69 - Alternative RCA programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true Alternative RCA programs. 400.69 Section 400.69 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee...

  15. Intrasplicing coordinates alternative first exons with alternative splicing in the protein 4.1R gene

    SciTech Connect

    Conboy, John G.; Parra, Marilyn K.; Tan, Jeff S.; Mohandas, Narla; Conboy, John G.

    2008-11-07

    In the protein 4.1R gene, alternative first exons splice differentially to alternative 3' splice sites far downstream in exon 2'/2 (E2'/2). We describe a novel intrasplicing mechanism by which exon 1A (E1A) splices exclusively to the distal E2'/2 acceptor via two nested splicing reactions regulated by novel properties of exon 1B (E1B). E1B behaves as an exon in the first step, using its consensus 5' donor to splice to the proximal E2'/2 acceptor. A long region of downstream intron is excised, juxtaposing E1B with E2'/2 to generate a new composite acceptor containing the E1B branchpoint/pyrimidine tract and E2 distal 3' AG-dinucleotide. Next, the upstream E1A splices over E1B to this distal acceptor, excising the remaining intron plus E1B and E2' to form mature E1A/E2 product. We mapped branch points for both intrasplicing reactions and demonstrated that mutation of the E1B 5' splice site or branchpoint abrogates intrasplicing. In the 4.1R gene, intrasplicing ultimately determines N-terminal protein structure and function. More generally, intrasplicing represents a new mechanism whereby alternative promoters can be coordinated with downstream alternative splicing.

  16. Program Health Indicators: An Alternative Approach to Program Review.

    ERIC Educational Resources Information Center

    Morton, John F.

    For the past 20 years, the University of Hawaii Community Colleges have had a formal process for the review of educational programs, requiring that each program undergo a systematic review at least once every 5 years. The result tended to be a lengthy document that chronicled the history of the previous 5 years and was far more descriptive than…

  17. Alternative RNA Structure-Coupled Gene Regulations in Tumorigenesis

    PubMed Central

    Chen, Feng-Chi

    2014-01-01

    Alternative RNA structures (ARSs), or alternative transcript isoforms, are critical for regulating cellular phenotypes in humans. In addition to generating functionally diverse protein isoforms from a single gene, ARS can alter the sequence contents of 5'/3' untranslated regions (UTRs) and intronic regions, thus also affecting the regulatory effects of these regions. ARS may introduce premature stop codon(s) into a transcript, and render the transcript susceptible to nonsense-mediated decay, which in turn can influence the overall gene expression level. Meanwhile, ARS can regulate the presence/absence of upstream open reading frames and microRNA targeting sites in 5'UTRs and 3'UTRs, respectively, thus affecting translational efficiencies and protein expression levels. Furthermore, since ARS may alter exon-intron structures, it can influence the biogenesis of intronic microRNAs and indirectly affect the expression of the target genes of these microRNAs. The connections between ARS and multiple regulatory mechanisms underline the importance of ARS in determining cell fate. Accumulating evidence indicates that ARS-coupled regulations play important roles in tumorigenesis. Here I will review our current knowledge in this field, and discuss potential future directions. PMID:25551597

  18. Alternative Approaches in Gene Discovery and Characterization in Alzheimer's Disease.

    PubMed

    Ertekin-Taner, Nilüfer; De Jager, Phillip L; Yu, Lei; Bennett, David A

    2013-03-01

    Uncovering the genetic risk and protective factors for complex diseases is of fundamental importance for advancing therapeutic and biomarker discoveries. This endeavor is particularly challenging for neuropsychiatric diseases where diagnoses predominantly rely on the clinical presentation, which may be heterogeneous, possibly due to the heterogeneity of the underlying genetic susceptibility factors and environmental exposures. Although genome-wide association studies of various neuropsychiatric diseases have recently identified susceptibility loci, there likely remain additional genetic risk factors that underlie the liability to these conditions. Furthermore, identification and characterization of the causal risk variant(s) in each of these novel susceptibility loci constitute a formidable task, particularly in the absence of any prior knowledge about their function or mechanism of action. Biologically relevant, quantitative phenotypes, i.e., endophenotypes, provide a powerful alternative to the more traditional, binary disease phenotypes in the discovery and characterization of susceptibility genes for neuropsychiatric conditions. In this review, we focus on Alzheimer's disease (AD) as a model neuropsychiatric disease and provide a synopsis of the recent literature on the use of endophenotypes in AD genetics. We highlight gene expression, neuropathology and cognitive endophenotypes in AD, with examples demonstrating the utility of these alternative approaches in the discovery of novel susceptibility genes and pathways. In addition, we discuss how these avenues generate testable hypothesis about the pathophysiology of genetic factors that have far-reaching implications for therapies. PMID:23482655

  19. Alternative management and funding options for aeronautics programs, Task 1

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Research and technology will be at lower program levels with basic military research for aviation decreasing as fewer aircraft programs are initiated and the present new aircraft programs move into the prototype and production status. The key question is can industry take on the management and financing role and meet the criteria and characteristics considered essential for a viable research and technology program. The criteria for evaluating alternative approaches include an examination of the nature of the product to be provided, responsiveness to changing needs, efficiency in terms of costs, ability to provide leadership, and to provide impartial and independent evaluation of approaches, and to provide technological inputs for regulating functions.

  20. ModMAP (Modular Multiple Alternatives Program). Program Description 1973-1974.

    ERIC Educational Resources Information Center

    Bridgeport Univ., CT. Coll. of Education.

    The Modular Multiple Alternatives Program (ModMaP), being developed at the University of Bridgeport, is the first competency-based, individualized graduate program in teacher education in the state of Connecticut. The program focuses on elementary teacher training and includes both master's and six-year level options. The program provides the…

  1. Alternative Route Programs for Certification in Special Education: Program Infrastructure Instructional Delivery, and Participant Characteristics

    ERIC Educational Resources Information Center

    Rosenberg, Michael S.; Boyer, K. Lynn; Sindelar, Paul T.; Misra, Sunil K.

    2007-01-01

    This study describes special education alternative route (AR) teacher preparation programs. The authors developed a national database of programs and collected information on program sponsorship, length and intensity, features, and participant demographics. Most of the 235 programs in the database were in states that had significant shortages of…

  2. GAP: A computer program for gene assembly

    SciTech Connect

    Eisnstein, J.R.; Uberbacher, E.C.; Guan, X.; Mural, R.J.; Mann, R.C.

    1991-09-01

    A computer program, GAP (Gene Assembly Program), has been written to assemble and score hypothetical genes, given a DNA sequence containing the gene, and the outputs of several other programs which analyze the sequence. These programs include the codign-recognition and splice-junction-recognition modules developed in this laboratory. GAP is a prototype of a planned system in which it will be integrated with an expert system and rule base. Initial tests of GAP have been carried out with four sequences, the exons of which have been determined by biochemcial methods. The highest-scoring hypothetical genes for each of the four sequences had percent correct splice junctions ranging from 50 to 100% (average 81%) and percent correct bases ranging from 92 to 100% (average 96%). 9 refs., 1 tab.

  3. Evaluating the Prevention through Alternative Learning Styles Program

    ERIC Educational Resources Information Center

    Huber, Mary J.; Workman, Judson; Ford, Jo Ann; Moore, Dennis; Mayer, Theresa

    2009-01-01

    This article reports on the evaluation of a two-year alcohol, tobacco and other drug (ATOD) intervention, the Prevention through Alternative Learning Styles ("PALS") program, targeting both teachers and middle-school students. Teachers are taught to recognize students' unique learning styles in the context of the ATOD curriculum and adapt the ATOD…

  4. Educational Alternatives for Boating Safety Programs. Final Report.

    ERIC Educational Resources Information Center

    Sager, E.; And Others

    The Coast Guard, in efforts to improve the safety of recreational boating, undertook research to identify educational alternatives in boating safety programs. Background research was done to assess materials from areas of boating education and education in comparable recreational areas. Research was also conducted to review educational and mass…

  5. 33 CFR 106.135 - Alternative Security Program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Alternative Security Program. 106.135 Section 106.135 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MARITIME SECURITY MARINE SECURITY: OUTER CONTINENTAL SHELF (OCS) FACILITIES General § 106.135...

  6. 33 CFR 106.135 - Alternative Security Program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Alternative Security Program. 106.135 Section 106.135 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MARITIME SECURITY MARINE SECURITY: OUTER CONTINENTAL SHELF (OCS) FACILITIES General § 106.135...

  7. 6 CFR 27.235 - Alternative security program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... facilities may submit an Alternate Security Program (ASP) pursuant to the requirements of this section. The... submit an ASP in lieu of a Security Vulnerability Assessment, Site Security Plan, or both. (2) Tier 1, Tier 2, or Tier 3 facilities may submit an ASP in lieu of a Site Security Plan. Tier 1, Tier 2,...

  8. 6 CFR 27.235 - Alternative security program.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... facilities may submit an Alternate Security Program (ASP) pursuant to the requirements of this section. The... submit an ASP in lieu of a Security Vulnerability Assessment, Site Security Plan, or both. (2) Tier 1, Tier 2, or Tier 3 facilities may submit an ASP in lieu of a Site Security Plan. Tier 1, Tier 2,...

  9. 6 CFR 27.235 - Alternative security program.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... facilities may submit an Alternate Security Program (ASP) pursuant to the requirements of this section. The... submit an ASP in lieu of a Security Vulnerability Assessment, Site Security Plan, or both. (2) Tier 1, Tier 2, or Tier 3 facilities may submit an ASP in lieu of a Site Security Plan. Tier 1, Tier 2,...

  10. 6 CFR 27.235 - Alternative security program.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... facilities may submit an Alternate Security Program (ASP) pursuant to the requirements of this section. The... submit an ASP in lieu of a Security Vulnerability Assessment, Site Security Plan, or both. (2) Tier 1, Tier 2, or Tier 3 facilities may submit an ASP in lieu of a Site Security Plan. Tier 1, Tier 2,...

  11. Alternate Learning Center. Abstracts of Inservice Training Programs.

    ERIC Educational Resources Information Center

    Rhode Island State Dept. of Education, Providence. Div. of Development and Operations.

    This booklet is a collection of abstracts describing the 18 programs offered at the Alternate Learning Center of the Rhode Island Teacher Center which has as its Primary function school based inservice training for local teachers and administrators. Each project is described in detail, including course goals, specific objectives, training…

  12. 33 CFR 106.135 - Alternative Security Program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Alternative Security Program. 106.135 Section 106.135 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MARITIME SECURITY MARINE SECURITY: OUTER CONTINENTAL SHELF (OCS) FACILITIES General § 106.135...

  13. The Evolution of Discipline: Alternative to Suspension Programs.

    ERIC Educational Resources Information Center

    Streva, Michael A.

    Historically, the chosen method of discipline in United States schools has gone from corporal punishment to an emphasis on suspension. Recently, as principals have become less certain about the extent of their authority to suspend students, many inschool programs have been developed as alternatives to suspension. Among the advantages of such…

  14. Alternative Administrative Certification: Socializing Factors Influencing Program Choice

    ERIC Educational Resources Information Center

    Bickmore, Dana L.; Bickmore, Steven T.; Raines, Sarah

    2013-01-01

    This study used an organizational socialization lens to examine factors influencing participants' decision to pursue the principalship and choice to engage in an alternate administration certification program. Through an analysis of participant focus groups and interviews, factors emerged from the codes that were compared with dimensions of…

  15. The Saturday Alternative: A Program for At-Risk Youth.

    ERIC Educational Resources Information Center

    Cooley, Van E.; Thompson, Jay C., Jr.

    The Saturday School Alternative for at-risk students provides educators with a realistic framework, time, and community resources to help resolve almost any student problem encountered. Based on effective schools research, the program is no panacea but a realistic attempt to modify student behavior by providing experiences that will build a…

  16. Disciplinary Alternative Education Programs in Texas--A 2009 Update

    ERIC Educational Resources Information Center

    Cortez, Albert; Cortez, Josie Danini

    2009-01-01

    This report discusses the issues surrounding the disciplinary alternative education programs (DAEPs) in Texas, its updated findings, and the authors' recommendations on what the state should do to address DAEP problems. DAEPs were supposed to be for criminal offenses--drug-related activities, gun violations and assault--all violations that had…

  17. Another Alternative: A 90-Day Contractual Detoxification Treatment Program

    ERIC Educational Resources Information Center

    Kahn, Robert B.; And Others

    1978-01-01

    In May 1974, Fresno County's Narcotic Abuse Treatment Program began a 21-day outpatient methadone detoxification treatment modality. The purpose of this paper is to examine this alternative treatment modality, its characteristics, its therapeutic outcomes and the rationale for its use. (Author)

  18. The human XPG gene: gene architecture, alternative splicing and single nucleotide polymorphisms

    PubMed Central

    Emmert, Steffen; Schneider, Thomas D.; Khan, Sikandar G.; Kraemer, Kenneth H.

    2001-01-01

    Defects in the XPG DNA repair endonuclease gene can result in the cancer-prone disorders xeroderma pigmentosum (XP) or the XP–Cockayne syndrome complex. While the XPG cDNA sequence was known, determination of the genomic sequence was required to understand its different functions. In cells from normal donors, we found that the genomic sequence of the human XPG gene spans 30 kb, contains 15 exons that range from 61 to 1074 bp and 14 introns that range from 250 to 5763 bp. Analysis of the splice donor and acceptor sites using an information theory-based approach revealed three splice sites with low information content, which are components of the minor (U12) spliceosome. We identified six alternatively spliced XPG mRNA isoforms in cells from normal donors and from XPG patients: partial deletion of exon 8, partial retention of intron 8, two with alternative exons (in introns 1 and 6) and two that retained complete introns (introns 3 and 9). The amount of alternatively spliced XPG mRNA isoforms varied in different tissues. Most alternative splice donor and acceptor sites had a relatively high information content, but one has the U12 spliceosome sequence. A single nucleotide polymorphism has allele frequencies of 0.74 for 3507G and 0.26 for 3507C in 91 donors. The human XPG gene contains multiple splice sites with low information content in association with multiple alternatively spliced isoforms of XPG mRNA. PMID:11266544

  19. 78 FR 14843 - Temporary Scope Expansion of the Post-Investigation Alternative Dispute Resolution Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-07

    ... COMMISSION Temporary Scope Expansion of the Post-Investigation Alternative Dispute Resolution Program AGENCY... Regulatory Commission (NRC) is expanding the scope of the post-investigation Alternative Dispute Resolution...-0115, ``Alternative Dispute Resolution Review Team (ART) Pilot Program Recommendations for...

  20. Considerations for the Placement of Youth with EBD in Alternative Education Programs

    ERIC Educational Resources Information Center

    Atkins, Trent; Bartuska, Jamie

    2010-01-01

    Alternative education programs (also called alternative schools) first appeared on the American landscape in the 1960s. Despite the proliferation of these programs, a generic description of what constitutes an alternative education program, historically, has been elusive. Most alternative education programs have the general criteria of serving…

  1. The Grimes Alternative School Program and Home Remedies Program. Interim Evaluation Report.

    ERIC Educational Resources Information Center

    Williams, Jay R.

    This report describes and evaluates two programs which deal with chronic status offenders: the Grimes Alternative School Program, a delinquency prevention project which targets intermediate school (grades 4 and 5) students with chronic discipline and/or truant behaviors, and the Home Remedies Program, a short-term crisis intervention and…

  2. Alternative pre-mRNA splicing switches modulate gene expression in late erythropoiesis

    SciTech Connect

    Yamamoto, Miki L.; Clark, Tyson A.; Gee, Sherry L.; Kang, Jeong-Ah; Schweitzer, Anthony C.; Wickrema, Amittha; Conboy, John G.

    2009-02-03

    Differentiating erythroid cells execute a unique gene expression program that insures synthesis of the appropriate proteome at each stage of maturation. Standard expression microarrays provide important insight into erythroid gene expression but cannot detect qualitative changes in transcript structure, mediated by RNA processing, that alter structure and function of encoded proteins. We analyzed stage-specific changes in the late erythroid transcriptome via use of high-resolution microarrays that detect altered expression of individual exons. Ten differentiation-associated changes in erythroblast splicing patterns were identified, including the previously known activation of protein 4.1R exon 16 splicing. Six new alternative splicing switches involving enhanced inclusion of internal cassette exons were discovered, as well as 3 changes in use of alternative first exons. All of these erythroid stage-specific splicing events represent activated inclusion of authentic annotated exons, suggesting they represent an active regulatory process rather than a general loss of splicing fidelity. The observation that 3 of the regulated transcripts encode RNA binding proteins (SNRP70, HNRPLL, MBNL2) may indicate significant changes in the RNA processing machinery of late erythroblasts. Together, these results support the existence of a regulated alternative pre-mRNA splicing program that is critical for late erythroid differentiation.

  3. Radiopharmaceutical and Gene Therapy Program

    SciTech Connect

    Buchsbaum, Donald J.

    2006-02-09

    The objective of our research program was to determine whether novel receptors can be induced in solid cancers as a target for therapy with radiolabeled unmodified peptides that bind to the receptors. The hypothesis was that induction of a high number of receptors on the surface of these cancer cells would result in an increased uptake of the radiolabeled monomeric peptides as compared to published results with radiolabeled antibodies or peptides to naturally expressed antigens or receptors, and therefore a better therapeutic outcome. The following is a summary of published results.

  4. "Programmed packaging" for gene delivery.

    PubMed

    Hyodo, M; Sakurai, Y; Akita, H; Harashima, H

    2014-11-10

    We report on the development of a multifunctional envelope-type nano device (MEND) based on our packaging concept "Programmed packaging" to control not only intracellular trafficking but also the biodistribution of encapsulated compounds such as nucleic acids/proteins/peptides. Our strategy for achieving this is based on molecular mechanisms of cell biology such as endocytosis, vesicular trafficking, etc. In this review, we summarize the concept of programmed packaging and discuss some of our recent successful examples of using MENDs. Systematic evolution of ligands by exponential enrichment (SELEX) was applied as a new methodology for identifying a new ligand toward cell or mitochondria. The delivery of siRNA to tumors and the tumor vasculature was achieved using pH sensitive lipid (YSK05), which was newly designed and optimized under in vivo conditions. The efficient delivery of pDNA to immune cells such as dendritic cells has also been developed using the KALA ligand, which can be a breakthrough technology for DNA vaccine. Finally, ss-cleavable and pH-activated lipid-like surfactant (ssPalm) which is a lipid like material with pH-activatable and SS-cleavable properties is also introduced as a proof of our concept. PMID:24780263

  5. Automated Eukaryotic Gene Structure Annotation Using EVidenceModeler and the Program to Assemble Spliced Alignments

    SciTech Connect

    Haas, B J; Salzberg, S L; Zhu, W; Pertea, M; Allen, J E; Orvis, J; White, O; Buell, C R; Wortman, J R

    2007-12-10

    EVidenceModeler (EVM) is presented as an automated eukaryotic gene structure annotation tool that reports eukaryotic gene structures as a weighted consensus of all available evidence. EVM, when combined with the Program to Assemble Spliced Alignments (PASA), yields a comprehensive, configurable annotation system that predicts protein-coding genes and alternatively spliced isoforms. Our experiments on both rice and human genome sequences demonstrate that EVM produces automated gene structure annotation approaching the quality of manual curation.

  6. ASPic-GeneID: A Lightweight Pipeline for Gene Prediction and Alternative Isoforms Detection

    PubMed Central

    Alioto, Tyler; Picardi, Ernesto; Guigó, Roderic

    2013-01-01

    New genomes are being sequenced at an increasingly rapid rate, far outpacing the rate at which manual gene annotation can be performed. Automated genome annotation is thus necessitated by this growth in genome projects; however, full-fledged annotation systems are usually home-grown and customized to a particular genome. There is thus a renewed need for accurate ab initio gene prediction methods. However, it is apparent that fully ab initio methods fall short of the required level of sensitivity and specificity for a quality annotation. Evidence in the form of expressed sequences gives the single biggest improvement in accuracy when used to inform gene predictions. Here, we present a lightweight pipeline for first-pass gene prediction on newly sequenced genomes. The two main components are ASPic, a program that derives highly accurate, albeit not necessarily complete, EST-based transcript annotations from EST alignments, and GeneID, a standard gene prediction program, which we have modified to take as evidence intron annotations. The introns output by ASPic CDS predictions is given to GeneID to constrain the exon-chaining process and produce predictions consistent with the underlying EST alignments. The pipeline was successfully tested on the entire C. elegans genome and the 44 ENCODE human pilot regions. PMID:24308000

  7. ASPic-GeneID: a lightweight pipeline for gene prediction and alternative isoforms detection.

    PubMed

    Alioto, Tyler; Picardi, Ernesto; Guigó, Roderic; Pesole, Graziano

    2013-01-01

    New genomes are being sequenced at an increasingly rapid rate, far outpacing the rate at which manual gene annotation can be performed. Automated genome annotation is thus necessitated by this growth in genome projects; however, full-fledged annotation systems are usually home-grown and customized to a particular genome. There is thus a renewed need for accurate ab initio gene prediction methods. However, it is apparent that fully ab initio methods fall short of the required level of sensitivity and specificity for a quality annotation. Evidence in the form of expressed sequences gives the single biggest improvement in accuracy when used to inform gene predictions. Here, we present a lightweight pipeline for first-pass gene prediction on newly sequenced genomes. The two main components are ASPic, a program that derives highly accurate, albeit not necessarily complete, EST-based transcript annotations from EST alignments, and GeneID, a standard gene prediction program, which we have modified to take as evidence intron annotations. The introns output by ASPic CDS predictions is given to GeneID to constrain the exon-chaining process and produce predictions consistent with the underlying EST alignments. The pipeline was successfully tested on the entire C. elegans genome and the 44 ENCODE human pilot regions. PMID:24308000

  8. Feelings of Preparedness among Alternatively Certified Teachers: What Is the Role of Program Features?

    ERIC Educational Resources Information Center

    Kee, Ayana N.

    2012-01-01

    In the United States, about one third of new teachers being hired are drawn from alternative certification programs. One way to address controversy about the differences among the training experiences of teachers in traditional certification programs, fast-track alternative programs, and residency alternative programs is to examine teacher reports…

  9. Discovering Successful Elements of Alternative Schools by Studying Student Perspectives in Two Alternative-to-Expulsion Programs

    ERIC Educational Resources Information Center

    Eschen, Eric Thomas

    2013-01-01

    This qualitative study analyzed two alternative-to-expulsion programs for high school students. Both programs, the "Learning with Peers Program" and the "Individualized Online Program", offered students an opportunity to earn credits rather than be expelled, but their philosophies presented an educational dichotomy: Should…

  10. The Evolutionary Fate of Alternatively Spliced Homologous Exons after Gene Duplication

    PubMed Central

    Abascal, Federico; Tress, Michael L.; Valencia, Alfonso

    2015-01-01

    Alternative splicing and gene duplication are the two main processes responsible for expanding protein functional diversity. Although gene duplication can generate new genes and alternative splicing can introduce variation through alternative gene products, the interplay between the two processes is complex and poorly understood. Here, we have carried out a study of the evolution of alternatively spliced exons after gene duplication to better understand the interaction between the two processes. We created a manually curated set of 97 human genes with mutually exclusively spliced homologous exons and analyzed the evolution of these exons across five distantly related vertebrates (lamprey, spotted gar, zebrafish, fugu, and coelacanth). Most of these exons had an ancient origin (more than 400 Ma). We found examples supporting two extreme evolutionary models for the behaviour of homologous axons after gene duplication. We observed 11 events in which gene duplication was accompanied by splice isoform separation, that is, each paralog specifically conserved just one distinct ancestral homologous exon. At other extreme, we identified genes in which the homologous exons were always conserved within paralogs, suggesting that the alternative splicing event cannot easily be separated from the function in these genes. That many homologous exons fall in between these two extremes highlights the diversity of biological systems and suggests that the subtle balance between alternative splicing and gene duplication is adjusted to the specific cellular context of each gene. PMID:25931610

  11. The evolutionary fate of alternatively spliced homologous exons after gene duplication.

    PubMed

    Abascal, Federico; Tress, Michael L; Valencia, Alfonso

    2015-06-01

    Alternative splicing and gene duplication are the two main processes responsible for expanding protein functional diversity. Although gene duplication can generate new genes and alternative splicing can introduce variation through alternative gene products, the interplay between the two processes is complex and poorly understood. Here, we have carried out a study of the evolution of alternatively spliced exons after gene duplication to better understand the interaction between the two processes. We created a manually curated set of 97 human genes with mutually exclusively spliced homologous exons and analyzed the evolution of these exons across five distantly related vertebrates (lamprey, spotted gar, zebrafish, fugu, and coelacanth). Most of these exons had an ancient origin (more than 400 Ma). We found examples supporting two extreme evolutionary models for the behaviour of homologous axons after gene duplication. We observed 11 events in which gene duplication was accompanied by splice isoform separation, that is, each paralog specifically conserved just one distinct ancestral homologous exon. At other extreme, we identified genes in which the homologous exons were always conserved within paralogs, suggesting that the alternative splicing event cannot easily be separated from the function in these genes. That many homologous exons fall in between these two extremes highlights the diversity of biological systems and suggests that the subtle balance between alternative splicing and gene duplication is adjusted to the specific cellular context of each gene. PMID:25931610

  12. SSME engine system integration of the alternate turbopump program

    NASA Astrophysics Data System (ADS)

    Sander, E. J.; Leahy, J. C.

    1993-06-01

    The effect of the engine level integration of the alternate High Pressure Oxidizer Turbopump (HPOTP) on the performance of the Space Shuttle Main Engine (SSME) is examined with reference to the results of the current engine system ground test data analysis. In particular, attention is given to the observed steady state and transient mainstage engine system effects with respect to various engine hardware combinations. It is concluded that none of the engine system concerns eliminate the turbomump from consideration as a permanent addition to the flight program pending certification.

  13. SSME engine system integration of the alternate turbopump program

    NASA Technical Reports Server (NTRS)

    Sander, E. J.; Leahy, J. C.

    1993-01-01

    The effect of the engine level integration of the alternate High Pressure Oxidizer Turbopump (HPOTP) on the performance of the Space Shuttle Main Engine (SSME) is examined with reference to the results of the current engine system ground test data analysis. In particular, attention is given to the observed steady state and transient mainstage engine system effects with respect to various engine hardware combinations. It is concluded that none of the engine system concerns eliminate the turbomump from consideration as a permanent addition to the flight program pending certification.

  14. 77 FR 47375 - Applications for New Awards; Assistive Technology Alternative Financing Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ... Applications for New Awards; Assistive Technology Alternative Financing Program AGENCY: Office of Special... Assistive Technology Alternative Financing Program Notice Inviting Applications for New Awards for Fiscal... have the private financial resources to purchase the assistive technology (AT) they need. In...

  15. 78 FR 22026 - Alternative Transportation in Parks and Public Lands Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-12

    ... Federal Transit Administration Alternative Transportation in Parks and Public Lands Program AGENCY... of Federal land management agency transportation investments with public transportation systems that... in the document ``Alternative Transportation in Parks and Public Lands Program: Requirements...

  16. ISO New England: Results of Ancillary Service Pilot Programs, Alternative Technology Regulation Pilot Program and Demand Response Reserves Pilot Program

    SciTech Connect

    Lowell, Jon; Yoshimura, Henry

    2011-10-26

    This PowerPoint presentation compares performance of pilot program assets and generation resources in alternative technology regulation and demand response reserves for flywheels and residential electric thermal storage.

  17. Innovation in two alternative elementary school programs: why it works.

    PubMed

    Dyson, B; O'Sullivan, M

    1998-09-01

    The purpose of this paper was to describe and interpret the factors that supported and maintained curricular innovation at two alternative elementary schools. The two schools have Project Adventure as their curriculum focus and use the concepts of risk, challenge, trust, cooperation, and problem solving; briefing and debriefing; and personal goal-setting contracts as part of their schoolwide curriculum (Project Adventure, 1991). Data were collected through formal interviews with the physical education teachers, the principals, and four classroom teachers at each school. Nonparticipant observation, field notes, informal interviews, and document analysis were also conducted. Inductive analysis and constant comparison were used to analyze and organize the data throughout the research process. Five factors emerged that supported the implementation of this innovative physical education program: a shared vision, external support for the schools' programs, curricula integration, centrality of physical education, and shared decision making. Project Adventure at these schools provides an example of substantive curricular reform where physical education was a critical element in the school programs. PMID:9777661

  18. A dynamic intron retention program enriched in RNA processing genes regulates gene expression during terminal erythropoiesis

    PubMed Central

    Pimentel, Harold; Parra, Marilyn; Gee, Sherry L.; Mohandas, Narla; Pachter, Lior; Conboy, John G.

    2016-01-01

    Differentiating erythroblasts execute a dynamic alternative splicing program shown here to include extensive and diverse intron retention (IR) events. Cluster analysis revealed hundreds of developmentally-dynamic introns that exhibit increased IR in mature erythroblasts, and are enriched in functions related to RNA processing such as SF3B1 spliceosomal factor. Distinct, developmentally-stable IR clusters are enriched in metal-ion binding functions and include mitoferrin genes SLC25A37 and SLC25A28 that are critical for iron homeostasis. Some IR transcripts are abundant, e.g. comprising ∼50% of highly-expressed SLC25A37 and SF3B1 transcripts in late erythroblasts, and thereby limiting functional mRNA levels. IR transcripts tested were predominantly nuclear-localized. Splice site strength correlated with IR among stable but not dynamic intron clusters, indicating distinct regulation of dynamically-increased IR in late erythroblasts. Retained introns were preferentially associated with alternative exons with premature termination codons (PTCs). High IR was observed in disease-causing genes including SF3B1 and the RNA binding protein FUS. Comparative studies demonstrated that the intron retention program in erythroblasts shares features with other tissues but ultimately is unique to erythropoiesis. We conclude that IR is a multi-dimensional set of processes that post-transcriptionally regulate diverse gene groups during normal erythropoiesis, misregulation of which could be responsible for human disease. PMID:26531823

  19. A dynamic intron retention program enriched in RNA processing genes regulates gene expression during terminal erythropoiesis

    DOE PAGESBeta

    Pimentel, Harold; Parra, Marilyn; Gee, Sherry L.; Mohandas, Narla; Pachter, Lior; Conboy, John G.

    2015-11-03

    Differentiating erythroblasts execute a dynamic alternative splicing program shown here to include extensive and diverse intron retention (IR) events. Cluster analysis revealed hundreds of developmentallydynamic introns that exhibit increased IR in mature erythroblasts, and are enriched in functions related to RNA processing such as SF3B1 spliceosomal factor. Distinct, developmentally-stable IR clusters are enriched in metal-ion binding functions and include mitoferrin genes SLC25A37 and SLC25A28 that are critical for iron homeostasis. Some IR transcripts are abundant, e.g. comprising ~50% of highly-expressed SLC25A37 and SF3B1 transcripts in late erythroblasts, and thereby limiting functional mRNA levels. IR transcripts tested were predominantly nuclearlocalized. Splicemore » site strength correlated with IR among stable but not dynamic intron clusters, indicating distinct regulation of dynamically-increased IR in late erythroblasts. Retained introns were preferentially associated with alternative exons with premature termination codons (PTCs). High IR was observed in disease-causing genes including SF3B1 and the RNA binding protein FUS. Comparative studies demonstrated that the intron retention program in erythroblasts shares features with other tissues but ultimately is unique to erythropoiesis. Finally, we conclude that IR is a multi-dimensional set of processes that post-transcriptionally regulate diverse gene groups during normal erythropoiesis, misregulation of which could be responsible for human disease.« less

  20. A dynamic intron retention program enriched in RNA processing genes regulates gene expression during terminal erythropoiesis

    SciTech Connect

    Pimentel, Harold; Parra, Marilyn; Gee, Sherry L.; Mohandas, Narla; Pachter, Lior; Conboy, John G.

    2015-11-03

    Differentiating erythroblasts execute a dynamic alternative splicing program shown here to include extensive and diverse intron retention (IR) events. Cluster analysis revealed hundreds of developmentallydynamic introns that exhibit increased IR in mature erythroblasts, and are enriched in functions related to RNA processing such as SF3B1 spliceosomal factor. Distinct, developmentally-stable IR clusters are enriched in metal-ion binding functions and include mitoferrin genes SLC25A37 and SLC25A28 that are critical for iron homeostasis. Some IR transcripts are abundant, e.g. comprising ~50% of highly-expressed SLC25A37 and SF3B1 transcripts in late erythroblasts, and thereby limiting functional mRNA levels. IR transcripts tested were predominantly nuclearlocalized. Splice site strength correlated with IR among stable but not dynamic intron clusters, indicating distinct regulation of dynamically-increased IR in late erythroblasts. Retained introns were preferentially associated with alternative exons with premature termination codons (PTCs). High IR was observed in disease-causing genes including SF3B1 and the RNA binding protein FUS. Comparative studies demonstrated that the intron retention program in erythroblasts shares features with other tissues but ultimately is unique to erythropoiesis. Finally, we conclude that IR is a multi-dimensional set of processes that post-transcriptionally regulate diverse gene groups during normal erythropoiesis, misregulation of which could be responsible for human disease.

  1. A dynamic intron retention program enriched in RNA processing genes regulates gene expression during terminal erythropoiesis.

    PubMed

    Pimentel, Harold; Parra, Marilyn; Gee, Sherry L; Mohandas, Narla; Pachter, Lior; Conboy, John G

    2016-01-29

    Differentiating erythroblasts execute a dynamic alternative splicing program shown here to include extensive and diverse intron retention (IR) events. Cluster analysis revealed hundreds of developmentally-dynamic introns that exhibit increased IR in mature erythroblasts, and are enriched in functions related to RNA processing such as SF3B1 spliceosomal factor. Distinct, developmentally-stable IR clusters are enriched in metal-ion binding functions and include mitoferrin genes SLC25A37 and SLC25A28 that are critical for iron homeostasis. Some IR transcripts are abundant, e.g. comprising ∼50% of highly-expressed SLC25A37 and SF3B1 transcripts in late erythroblasts, and thereby limiting functional mRNA levels. IR transcripts tested were predominantly nuclear-localized. Splice site strength correlated with IR among stable but not dynamic intron clusters, indicating distinct regulation of dynamically-increased IR in late erythroblasts. Retained introns were preferentially associated with alternative exons with premature termination codons (PTCs). High IR was observed in disease-causing genes including SF3B1 and the RNA binding protein FUS. Comparative studies demonstrated that the intron retention program in erythroblasts shares features with other tissues but ultimately is unique to erythropoiesis. We conclude that IR is a multi-dimensional set of processes that post-transcriptionally regulate diverse gene groups during normal erythropoiesis, misregulation of which could be responsible for human disease. PMID:26531823

  2. An extensive program of periodic alternative splicing linked to cell cycle progression

    PubMed Central

    Dominguez, Daniel; Tsai, Yi-Hsuan; Weatheritt, Robert; Wang, Yang; Blencowe, Benjamin J; Wang, Zefeng

    2016-01-01

    Progression through the mitotic cell cycle requires periodic regulation of gene function at the levels of transcription, translation, protein-protein interactions, post-translational modification and degradation. However, the role of alternative splicing (AS) in the temporal control of cell cycle is not well understood. By sequencing the human transcriptome through two continuous cell cycles, we identify ~1300 genes with cell cycle-dependent AS changes. These genes are significantly enriched in functions linked to cell cycle control, yet they do not significantly overlap genes subject to periodic changes in steady-state transcript levels. Many of the periodically spliced genes are controlled by the SR protein kinase CLK1, whose level undergoes cell cycle-dependent fluctuations via an auto-inhibitory circuit. Disruption of CLK1 causes pleiotropic cell cycle defects and loss of proliferation, whereas CLK1 over-expression is associated with various cancers. These results thus reveal a large program of CLK1-regulated periodic AS intimately associated with cell cycle control. DOI: http://dx.doi.org/10.7554/eLife.10288.001 PMID:27015110

  3. 5 CFR 2424.10 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Alternative Dispute Resolution; Requesting and Providing Allegations Concerning the Duty To Bargain § 2424.10 Collaboration and Alternative Dispute Resolution Program. Where an exclusive representative and an agency are... and Alternative Dispute Resolution Program (CADR). Upon request, and as agreed upon by the...

  4. 5 CFR 2425.8 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AWARDS § 2425.8 Collaboration and Alternative Dispute Resolution Program. The parties may request assistance from the Collaboration and Alternative Dispute Resolution Program (CADR) to attempt to resolve the... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Collaboration and Alternative...

  5. 5 CFR 2424.10 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Alternative Dispute Resolution; Requesting and Providing Allegations Concerning the Duty To Bargain § 2424.10 Collaboration and Alternative Dispute Resolution Program. Where an exclusive representative and an agency are... and Alternative Dispute Resolution Program (CADR). Upon request, and as agreed upon by the...

  6. 5 CFR 2425.8 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Collaboration and Alternative Dispute... AWARDS § 2425.8 Collaboration and Alternative Dispute Resolution Program. The parties may request assistance from the Collaboration and Alternative Dispute Resolution Program (CADR) to attempt to resolve...

  7. 5 CFR 2425.8 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Collaboration and Alternative Dispute... AWARDS § 2425.8 Collaboration and Alternative Dispute Resolution Program. The parties may request assistance from the Collaboration and Alternative Dispute Resolution Program (CADR) to attempt to resolve...

  8. 5 CFR 2425.8 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Collaboration and Alternative Dispute... AWARDS § 2425.8 Collaboration and Alternative Dispute Resolution Program. The parties may request assistance from the Collaboration and Alternative Dispute Resolution Program (CADR) to attempt to resolve...

  9. Alternative Education Programs for At-Risk Youth: Issues, Best Practice, and Recommendations.

    ERIC Educational Resources Information Center

    Tobin, Tary; Sprague, Jeffrey

    1999-01-01

    This publication discusses definitions of alternative education, outlines common program features, and lists challenges to providing quality services. The paper focuses on alternatives for students who are at risk for school failure, dropout, or delinquency. Since few studies on the effectiveness of alternative programs have been conducted and…

  10. Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.

    PubMed

    Holm, Frida; Hellqvist, Eva; Mason, Cayla N; Ali, Shawn A; Delos-Santos, Nathaniel; Barrett, Christian L; Chun, Hye-Jung; Minden, Mark D; Moore, Richard A; Marra, Marco A; Runza, Valeria; Frazer, Kelly A; Sadarangani, Anil; Jamieson, Catriona H M

    2015-12-15

    Formative research suggests that a human embryonic stem cell-specific alternative splicing gene regulatory network, which is repressed by Muscleblind-like (MBNL) RNA binding proteins, is involved in cell reprogramming. In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transduction, and in vivo humanized mouse model studies demonstrated that malignant reprogramming of progenitors into self-renewing blast crisis chronic myeloid leukemia stem cells (BC LSCs) was partially driven by decreased MBNL3. Lentiviral knockdown of MBNL3 resulted in reversion to an embryonic alternative splice isoform program typified by overexpression of CD44 transcript variant 3, containing variant exons 8-10, and BC LSC proliferation. Although isoform-specific lentiviral CD44v3 overexpression enhanced chronic phase chronic myeloid leukemia (CML) progenitor replating capacity, lentiviral shRNA knockdown abrogated these effects. Combined treatment with a humanized pan-CD44 monoclonal antibody and a breakpoint cluster region - ABL proto-oncogene 1, nonreceptor tyrosine kinase (BCR-ABL1) antagonist inhibited LSC maintenance in a niche-dependent manner. In summary, MBNL3 down-regulation-related reversion to an embryonic alternative splicing program, typified by CD44v3 overexpression, represents a previously unidentified mechanism governing malignant progenitor reprogramming in malignant microenvironments and provides a pivotal opportunity for selective BC LSC detection and therapeutic elimination. PMID:26621726

  11. Alternatives Analysis for the Resumption of Transient Testing Program

    SciTech Connect

    Lee Nelson

    2013-11-01

    An alternatives analysis was performed for resumption of transient testing. The analysis considered eleven alternatives – including both US international facilities. A screening process was used to identify two viable alternatives from the original eleven. In addition, the alternatives analysis includes a no action alternative as required by the National Environmental Policy Act (NEPA). The alternatives considered in this analysis included: 1. Restart the Transient Reactor Test Facility (TREAT) 2. Modify the Annular Core Research Reactor (ACRR) which includes construction of a new hot cell and installation of a new hodoscope. 3. No Action

  12. Alternative Splicing of an Insect Sodium Channel Gene Generates Pharmacologically Distinct Sodium Channels

    PubMed Central

    Tan, Jianguo; Liu, Zhiqi; Nomura, Yoshiko; Goldin, Alan L.; Dong, Ke

    2011-01-01

    Alternative splicing is a major mechanism by which potassium and calcium channels increase functional diversity in animals. Extensive alternative splicing of the para sodium channel gene and developmental regulation of alternative splicing have been reported in Drosophila species. Alternative splicing has also been observed for several mammalian voltage-gated sodium channel genes. However, the functional significance of alternative splicing of sodium channels has not been demonstrated. In this study, we identified three mutually exclusive alternative exons encoding part of segments 3 and 4 of domain III in the German cockroach sodium channel gene, paraCSMA. The splice site is conserved in the mouse, fish, and human Nav1.6 sodium channel genes, suggesting an ancient origin. One of the alternative exons possesses a stop codon, which would generate a truncated protein with only the first two domains. The splicing variant containing the stop codon is detected only in the PNS, whereas the other two full-size variants were detected in both the PNS and CNS. When expressed in Xenopus oocytes, the two splicing variants produced robust sodium currents, but with different gating properties, whereas the splicing variant with the stop codon did not produce any detectable sodium current. Furthermore, these two functional splicing variants exhibited a striking difference in sensitivity to a pyrethroid insecticide, deltamethrin. Exon swapping partially reversed the channel sensitivity to deltamethrin. Our results therefore provide the first evidence that alternative splicing of a sodium channel gene produces pharmacologically distinct channels. PMID:12097481

  13. 77 FR 31756 - Energy Conservation Program: Alternative Efficiency Determination Methods and Alternative Rating...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-30

    ... Methods and Alternative Rating Methods: Public Meeting AGENCY: Office of Energy Efficiency and Renewable... proposed modifications to the regulations authorizing the use of alternative methods of determining energy... alternative methods of determining energy efficiency or energy consumption of various consumer products...

  14. Alternate Assessment Manual for the Arizona Student Achievement Program

    ERIC Educational Resources Information Center

    Arizona Department of Education, 2005

    2005-01-01

    The Alternate Assessment Code of Ethics informs school personnel involved in alternate assessments of ethical, nondiscriminatory assessment practices and underscores the diligence necessary to provide accurate assessment data for instructional decision-making. The importance of commitment and adherence to the Alternate Assessment Code of Ethics by…

  15. Computational Analysis of an Evolutionarily Conserved VertebrateMuscle Alternative Splicing Program

    SciTech Connect

    Das, Debopriya; Clark, Tyson A.; Schweitzer, Anthony; Marr,Henry; Yamamoto, Miki L.; Parra, Marilyn K.; Arribere, Josh; Minovitsky,Simon; Dubchak, Inna; Blume, John E.; Conboy, John G.

    2006-06-15

    A novel exon microarray format that probes gene expression with single exon resolution was employed to elucidate critical features of a vertebrate muscle alternative splicing program. A dataset of 56 microarray-defined, muscle-enriched exons and their flanking introns were examined computationally in order to investigate coordination of the muscle splicing program. Candidate intron regulatory motifs were required to meet several stringent criteria: significant over-representation near muscle-enriched exons, correlation with muscle expression, and phylogenetic conservation among genomes of several vertebrate orders. Three classes of regulatory motifs were identified in the proximal downstream intron, within 200nt of the target exons: UGCAUG, a specific binding site for Fox-1 related splicing factors; ACUAAC, a novel branchpoint-like element; and UG-/UGC-rich elements characteristic of binding sites for CELF splicing factors. UGCAUG was remarkably enriched, being present in nearly one-half of all cases. These studies suggest that Fox and CELF splicing factors play a major role in enforcing the muscle-specific alternative splicing program, facilitating expression of a set of unique isoforms of cytoskeletal proteins that are critical to muscle cell differentiation. Supplementary materials: There are four supplementary tables and one supplementary figure. The tables provide additional detailed information concerning the muscle-enriched datasets, and about over-represented oligonucleotide sequences in the flanking introns. The supplementary figure shows RT-PCR data confirming the muscle-enriched expression of exons predicted from the microarray analysis.

  16. 19 CFR 101.9 - Test programs or procedures; alternate requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false Test programs or procedures; alternate... SECURITY; DEPARTMENT OF THE TREASURY GENERAL PROVISIONS § 101.9 Test programs or procedures; alternate requirements. (a) General testing. For purposes of conducting a test program or procedure designed to...

  17. 19 CFR 101.9 - Test programs or procedures; alternate requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Test programs or procedures; alternate... SECURITY; DEPARTMENT OF THE TREASURY GENERAL PROVISIONS § 101.9 Test programs or procedures; alternate requirements. (a) General testing. For purposes of conducting a test program or procedure designed to...

  18. 19 CFR 101.9 - Test programs or procedures; alternate requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false Test programs or procedures; alternate... SECURITY; DEPARTMENT OF THE TREASURY GENERAL PROVISIONS § 101.9 Test programs or procedures; alternate requirements. (a) General testing. For purposes of conducting a test program or procedure designed to...

  19. 19 CFR 101.9 - Test programs or procedures; alternate requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Test programs or procedures; alternate... SECURITY; DEPARTMENT OF THE TREASURY GENERAL PROVISIONS § 101.9 Test programs or procedures; alternate requirements. (a) General testing. For purposes of conducting a test program or procedure designed to...

  20. 19 CFR 101.9 - Test programs or procedures; alternate requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false Test programs or procedures; alternate... SECURITY; DEPARTMENT OF THE TREASURY GENERAL PROVISIONS § 101.9 Test programs or procedures; alternate requirements. (a) General testing. For purposes of conducting a test program or procedure designed to...

  1. Perception of Preparedness of Novice Teachers from Alternative and Traditional Licensing Programs

    ERIC Educational Resources Information Center

    Buchanan, Tenielle; Lang, Nathan; Morin, Laura-Lee

    2013-01-01

    Alternative teacher licensing programs have become very popular; however, very little research has been conducted on the efficacy of the programs. Alternative licensing programs (ALPs) have evolved and multiplied rapidly over the last few decades. The Tennessee Independent Colleges and Universities Association (TICUA) is attempting to determine…

  2. Backgrounds, Teaching Responsibilities, and Motivations of Music Education Candidates Enrolled in Alternative Certification Music Education Programs

    ERIC Educational Resources Information Center

    Hellman, Daniel; McDowell, Carol

    2011-01-01

    The purpose of this study was to examine the factors associated with enrolling, continuing, and completing alternative certification programs in music education. Data were collected via surveys and semi-structured interviews of music teacher candidates from 43 program completers from seven alternative certification programs in three states. In…

  3. Structure of the human myelin/oligodendrocyte glycoprotein gene and multiple alternative spliced isoforms

    SciTech Connect

    Pham-Dinh, D.; Gaspera, D.B.; Dautigny, A.

    1995-09-20

    Myelin/oligodendrocyte glycoprotein (MOG), a special component of the central nervous system localization on the outermost lamellae of mature myelin, is a member of the immunoglobulin superfamily. We report here the organization of the human MOG gene, which spans approximately 17 kb, and the characterization of six MOG mRNA splicing variants. The intron/exon structure of the human MOG gene confirmed the splicing pattern, supporting the hypothesis that mRNA isoforms could arise by alternative splicing of a single gene. In addition to the eight exons coding for the major MOG isoform, the human MOG gene also contains 3` region, a previously unknown alternatively spliced coding exon, VIA. Alternative utilization of two acceptor splicing sites for exon VIII could produce two different C-termini. The nucleotide sequences presented here may be a useful tool to study further possible involvement if the MOG gene in hereditary neurological disorders. 23 refs., 5 figs.

  4. Alternative Transposition Generates New Chimeric Genes and Segmental Duplications at the Maize p1 Locus.

    PubMed

    Wang, Dafang; Yu, Chuanhe; Zuo, Tao; Zhang, Jianbo; Weber, David F; Peterson, Thomas

    2015-11-01

    The maize Ac/Ds transposon family was the first transposable element system identified and characterized by Barbara McClintock. Ac/Ds transposons belong to the hAT family of class II DNA transposons. We and others have shown that Ac/Ds elements can undergo a process of alternative transposition in which the Ac/Ds transposase acts on the termini of two separate, nearby transposons. Because these termini are present in different elements, alternative transposition can generate a variety of genome alterations such as inversions, duplications, deletions, and translocations. Moreover, Ac/Ds elements transpose preferentially into genic regions, suggesting that structural changes arising from alternative transposition may potentially generate chimeric genes at the rearrangement breakpoints. Here we identified and characterized 11 independent cases of gene fusion induced by Ac alternative transposition. In each case, a functional chimeric gene was created by fusion of two linked, paralogous genes; moreover, each event was associated with duplication of the ∼70-kb segment located between the two paralogs. An extant gene in the maize B73 genome that contains an internal duplication apparently generated by an alternative transposition event was also identified. Our study demonstrates that alternative transposition-induced duplications may be a source for spontaneous creation of diverse genome structures and novel genes in maize. PMID:26434719

  5. Alternatives for Young Americans: A Catalog of Drug Abuse Prevention Programs.

    ERIC Educational Resources Information Center

    Applied Management Sciences, Inc., Silver Spring, MD.

    This reference book contains resumes of program descriptions geared toward youth and adults who need or want information on drug abuse prevention programs, locally or nationally. Several major entries include: (1) national program models; (2) evaluated alternative programs from all states; and (3) descriptions of prevention programs. All section…

  6. School District Program Cost Accounting: An Alternative Approach

    ERIC Educational Resources Information Center

    Hentschke, Guilbert C.

    1975-01-01

    Discusses the value for school districts of a program cost accounting system and examines different approaches to generating program cost data, with particular emphasis on the "cost allocation to program system" (CAPS) and the traditional "transaction-based system." (JG)

  7. Gene, Stem Cell, and Alternative Therapies for SCA 1

    PubMed Central

    Wagner, Jacob L.; O'Connor, Deirdre M.; Donsante, Anthony; Boulis, Nicholas M.

    2016-01-01

    Spinocerebellar ataxia 1 is an autosomal dominant disease characterized by neurodegeneration and motor dysfunction. In disease pathogenesis, polyglutamine expansion within Ataxin-1, a gene involved in transcriptional repression, causes protein nuclear inclusions to form. Most notably, neuronal dysfunction presents in Purkinje cells. However, the effect of mutant Ataxin-1 is not entirely understood. Two mouse models are employed to represent spinocerebellar ataxia 1, a B05 transgenic model that specifically expresses mutant Ataxin-1 in Purkinje cells, and a Sca1 154Q/2Q model that inserts the polyglutamine expansion into the mouse Ataxin-1 locus so that the mutant Ataxin-1 is expressed in all cells that express Ataxin-1. This review aims to summarize and evaluate the wide variety of therapies proposed for spinocerebellar ataxia 1, specifically gene and stem cell therapies. PMID:27570504

  8. Gene, Stem Cell, and Alternative Therapies for SCA 1.

    PubMed

    Wagner, Jacob L; O'Connor, Deirdre M; Donsante, Anthony; Boulis, Nicholas M

    2016-01-01

    Spinocerebellar ataxia 1 is an autosomal dominant disease characterized by neurodegeneration and motor dysfunction. In disease pathogenesis, polyglutamine expansion within Ataxin-1, a gene involved in transcriptional repression, causes protein nuclear inclusions to form. Most notably, neuronal dysfunction presents in Purkinje cells. However, the effect of mutant Ataxin-1 is not entirely understood. Two mouse models are employed to represent spinocerebellar ataxia 1, a B05 transgenic model that specifically expresses mutant Ataxin-1 in Purkinje cells, and a Sca1 154Q/2Q model that inserts the polyglutamine expansion into the mouse Ataxin-1 locus so that the mutant Ataxin-1 is expressed in all cells that express Ataxin-1. This review aims to summarize and evaluate the wide variety of therapies proposed for spinocerebellar ataxia 1, specifically gene and stem cell therapies. PMID:27570504

  9. Alternative splicing of the FMR1 gene in human fetal brain neurons

    SciTech Connect

    Tao Huang; Yan Shen; Xue-bin Qin; Guan-Yun Wu

    1996-08-09

    The alternative splicing expression of the FMR1 gene was reported in several human and mouse tissues. Five regions of FMR1 gene can be alternatively spliced, but the combination of them has not been investigated fully. We reported here the analysis of alternative splicing pattern of the FMR1 gene in cultured fetal human neurons, using a RT-PCR and cloning strategy. Eleven splicing types were cloned and different isoforms were not equally represented. The dominant isoform represents nearly 40%, and the other isoforms were relatively rare. One isoform has a different carboxyl-terminus. Most of the alternative spliced regions appear hydrophilic; thus, they may locate on the surface of the FMR1 protein. 16 refs., 2 figs.

  10. An alternative pathway for gene regulation by Myc.

    PubMed Central

    Peukert, K; Staller, P; Schneider, A; Carmichael, G; Hänel, F; Eilers, M

    1997-01-01

    The c-Myc protein activates transcription as part of a heteromeric complex with Max. However, Myc-transformed cells are characterized by loss of expression of several genes, suggesting that Myc may also repress gene expression. Two-hybrid cloning identifies a novel POZ domain Zn finger protein (Miz-1; Myc-interacting Zn finger protein-1) that specifically interacts with Myc, but not with Max or USF. Miz-1 binds to start sites of the adenovirus major late and cyclin D1 promoters and activates transcription from both promoters. Miz-1 has a potent growth arrest function. Binding of Myc to Miz-1 requires the helix-loop-helix domain of Myc and a short amphipathic helix located in the carboxy-terminus of Miz-1. Expression of Myc inhibits transactivation, overcomes Miz-1-induced growth arrest and renders Miz-1 insoluble in vivo. These processes depend on Myc and Miz-1 association and on the integrity of the POZ domain of Miz-1, suggesting that Myc binding activates a latent inhibitory function of this domain. Fusion of a nuclear localization signal induces efficient nuclear transport of Miz-1 and impairs the ability of Myc to overcome transcriptional activation and growth arrest by Miz-1. Our data suggest a model for how gene repression by Myc may occur in vivo. PMID:9312026

  11. Alternative filtration testing program: Pre-evaluation of test results

    SciTech Connect

    Georgeton, G.K.; Poirier, M.R.

    1990-09-28

    Based on results of testing eight solids removal technologies and one pretreatment option, it is recommended that a centrifugal ultrafilter and polymeric ultrafilter undergo further testing as possible alternatives to the Norton Ceramic filters. Deep bed filtration should be considered as a third alternative, if a backwashable cartridge filter is shown to be inefficient in separate testing.

  12. Supports and Enhancements Designed for Alternative School Programming

    ERIC Educational Resources Information Center

    Menendez, Anthony L.

    2007-01-01

    In this informational survey, the author briefly explores the needs of professional staff and administrators who serve students in alternative educational settings and special education and provides a listing of helpful Internet resources. Initially, the author provides general information concerning the current state of alternative education…

  13. Interweaving Teaching and Emotional Support for Novice Special Educators in Alternative Certification Programs

    ERIC Educational Resources Information Center

    Ricci, Leila Ansari; Zetlin, Andrea G.

    2013-01-01

    As the shortage of special education teachers has led to increasing numbers of teacher candidates enrolled in alternative certification programs, there is a need to provide systematic mentoring and coaching. The relationship between support providers and novice teachers enrolled in an alternative certification program in a diverse, urban…

  14. Considerations for Coursework and Mentoring in an Alternative-Route-to- Licensure Program for STEM Teachers

    ERIC Educational Resources Information Center

    Burbank, Mary D.; Shooter, Wynn; Groth, Cori A

    2014-01-01

    The purpose of this project was to investigate stakeholders' perspectives on coursework and the role of mentors within a unique teacher education program for prospective teachers of mathematics in an alternative-route-to-licensure program. The alternative route reflects a partnership between an institution of higher education, a state office…

  15. Bureaucratic Discretion and Alternative Teacher Certification: Understanding Program Variation in Missouri

    ERIC Educational Resources Information Center

    Heinen, Ethan B.; Scribner, Jay Paredes

    2007-01-01

    Alternative teacher certification literature has contributed significantly to our understanding of this approach to teacher preparation. However, this literature has more often than not treated alternative teacher certification programs (ATCPs) as a black box, thus ignoring program heterogeneity. The present study examines how and why five ATCPs…

  16. Evaluation of the Alternate Program at McBride School, 1971-72. Research Report.

    ERIC Educational Resources Information Center

    Gilbert, Katherine J.

    The alternate program at McBride School was an organizational and curricular modification that offered optional activities, community recreation, semestering of some subjects, and unstructured time. It involved four intermediate grade classes. Questionnaires were distributed to students who were enrolled in the alternate program and to their…

  17. 46 CFR 71.50-19 - The Alternative Hull Examination (AHE) Program application.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 3 2013-10-01 2013-10-01 false The Alternative Hull Examination (AHE) Program...) PASSENGER VESSELS INSPECTION AND CERTIFICATION Drydocking § 71.50-19 The Alternative Hull Examination (AHE... apply to the AHE Program. You must submit an application at least 90 days before the requested...

  18. 46 CFR 176.630 - The Alternative Hull Examination (AHE) Program application.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false The Alternative Hull Examination (AHE) Program... PASSENGER VESSELS (UNDER 100 GROSS TONS) INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 176.630 The Alternative Hull Examination (AHE) Program application. If your vessel meets the...

  19. 46 CFR 71.50-19 - The Alternative Hull Examination (AHE) Program application.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 3 2014-10-01 2014-10-01 false The Alternative Hull Examination (AHE) Program...) PASSENGER VESSELS INSPECTION AND CERTIFICATION Drydocking § 71.50-19 The Alternative Hull Examination (AHE... apply to the AHE Program. You must submit an application at least 90 days before the requested...

  20. 46 CFR 176.630 - The Alternative Hull Examination (AHE) Program application.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false The Alternative Hull Examination (AHE) Program... PASSENGER VESSELS (UNDER 100 GROSS TONS) INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 176.630 The Alternative Hull Examination (AHE) Program application. If your vessel meets the...

  1. 46 CFR 71.50-31 - Continued participation in the Alternative Hull Examination (AHE) program.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 3 2011-10-01 2011-10-01 false Continued participation in the Alternative Hull... the Alternative Hull Examination (AHE) program. (a) To continue to participate in the AHE Program, vessel operators must conduct an annual hull condition assessment. At a minimum, vessel operators...

  2. 46 CFR 71.50-19 - The Alternative Hull Examination (AHE) Program application.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 3 2012-10-01 2012-10-01 false The Alternative Hull Examination (AHE) Program...) PASSENGER VESSELS INSPECTION AND CERTIFICATION Drydocking § 71.50-19 The Alternative Hull Examination (AHE... apply to the AHE Program. You must submit an application at least 90 days before the requested...

  3. 46 CFR 71.50-19 - The Alternative Hull Examination (AHE) Program application.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false The Alternative Hull Examination (AHE) Program...) PASSENGER VESSELS INSPECTION AND CERTIFICATION Drydocking § 71.50-19 The Alternative Hull Examination (AHE... apply to the AHE Program. You must submit an application at least 90 days before the requested...

  4. 46 CFR 176.630 - The Alternative Hull Examination (AHE) Program application.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false The Alternative Hull Examination (AHE) Program... PASSENGER VESSELS (UNDER 100 GROSS TONS) INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 176.630 The Alternative Hull Examination (AHE) Program application. If your vessel meets the...

  5. 46 CFR 71.50-31 - Continued participation in the Alternative Hull Examination (AHE) program.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 3 2013-10-01 2013-10-01 false Continued participation in the Alternative Hull... the Alternative Hull Examination (AHE) program. (a) To continue to participate in the AHE Program, vessel operators must conduct an annual hull condition assessment. At a minimum, vessel operators...

  6. 46 CFR 71.50-31 - Continued participation in the Alternative Hull Examination (AHE) program.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 3 2012-10-01 2012-10-01 false Continued participation in the Alternative Hull... the Alternative Hull Examination (AHE) program. (a) To continue to participate in the AHE Program, vessel operators must conduct an annual hull condition assessment. At a minimum, vessel operators...

  7. 46 CFR 71.50-31 - Continued participation in the Alternative Hull Examination (AHE) program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 3 2014-10-01 2014-10-01 false Continued participation in the Alternative Hull... the Alternative Hull Examination (AHE) program. (a) To continue to participate in the AHE Program, vessel operators must conduct an annual hull condition assessment. At a minimum, vessel operators...

  8. Emotionally Disturbed Children: A Program of Alternatives to Residential Treatment

    ERIC Educational Resources Information Center

    Bedford, Linda; Hybertson, Larry D.

    1975-01-01

    Describes the Treatment Alternatives Project (TAP) which provides caseworker aid and supportive services that allow problem children to undergo treatment while remaining in their own homes or foster homes. (SDH)

  9. Design and Implementation Considerations for Alternative Teacher Compensation Programs

    ERIC Educational Resources Information Center

    Brodsky, Andrew; DeCesare, Dale; Kramer-Wine, Jennifer

    2010-01-01

    Over the past decade, educators and policymakers have used a variety of approaches to designing and implementing teacher compensation programs. These approaches include federal incentive funds, state-level programs, and district initiatives. This article reviews 6 such programs in order to identify themes and draw conclusions relevant to…

  10. Alternative Summer Educational Programs for Urban Hearing-Impaired Children.

    ERIC Educational Resources Information Center

    Goldfarb, Mark; Israelson, Jo

    The paper describes the summer programs offered by Kendall Demonstration Elementary School, a year-round day school for elementary level hearing impaired students in Washington, D.C. The programs are explained to be based on a three-point philosophy: (1) summer programs should be different from those of the rest of the year; (2) summer programs…

  11. Reconstruction and minimal gene requirements for the alternative iron-only nitrogenase in Escherichia coli.

    PubMed

    Yang, Jianguo; Xie, Xiaqing; Wang, Xia; Dixon, Ray; Wang, Yi-Ping

    2014-09-01

    All diazotrophic organisms sequenced to date encode a molybdenum-dependent nitrogenase, but some also have alternative nitrogenases that are dependent on either vanadium (VFe) or iron only (FeFe) for activity. In Azotobacter vinelandii, expression of the three different types of nitrogenase is regulated in response to metal availability. The majority of genes required for nitrogen fixation in this organism are encoded in the nitrogen fixation (nif) gene clusters, whereas genes specific for vanadium- or iron-dependent diazotophy are encoded by the vanadium nitrogen fixation (vnf) and alternative nitrogen fixation (anf) genes, respectively. Due to the complexities of metal-dependent regulation and gene redundancy in A. vinelandii, it has been difficult to determine the precise genetic requirements for alternative nitrogen fixation. In this study, we have used Escherichia coli as a chassis to build an artificial iron-only (Anf) nitrogenase system composed of defined anf and nif genes. Using this system, we demonstrate that the pathway for biosynthesis of the iron-only cofactor (FeFe-co) is likely to be simpler than the pathway for biosynthesis of the molybdenum-dependent cofactor (FeMo-co) equivalent. A number of genes considered to be essential for nitrogen fixation by FeFe nitrogenase, including nifM, vnfEN, and anfOR, are not required for the artificial Anf system in E. coli. This finding has enabled us to engineer a minimal FeFe nitrogenase system comprising the structural anfHDGK genes and the nifBUSV genes required for metallocluster biosynthesis, with nifF and nifJ providing electron transport to the alternative nitrogenase. This minimal Anf system has potential implications for engineering diazotrophy in eukaryotes, particularly in compartments (e.g., organelles) where molybdenum may be limiting. PMID:25139995

  12. Reconstruction and minimal gene requirements for the alternative iron-only nitrogenase in Escherichia coli

    PubMed Central

    Yang, Jianguo; Xie, Xiaqing; Wang, Xia; Dixon, Ray; Wang, Yi-Ping

    2014-01-01

    All diazotrophic organisms sequenced to date encode a molybdenum-dependent nitrogenase, but some also have alternative nitrogenases that are dependent on either vanadium (VFe) or iron only (FeFe) for activity. In Azotobacter vinelandii, expression of the three different types of nitrogenase is regulated in response to metal availability. The majority of genes required for nitrogen fixation in this organism are encoded in the nitrogen fixation (nif) gene clusters, whereas genes specific for vanadium- or iron-dependent diazotophy are encoded by the vanadium nitrogen fixation (vnf) and alternative nitrogen fixation (anf) genes, respectively. Due to the complexities of metal-dependent regulation and gene redundancy in A. vinelandii, it has been difficult to determine the precise genetic requirements for alternative nitrogen fixation. In this study, we have used Escherichia coli as a chassis to build an artificial iron-only (Anf) nitrogenase system composed of defined anf and nif genes. Using this system, we demonstrate that the pathway for biosynthesis of the iron-only cofactor (FeFe-co) is likely to be simpler than the pathway for biosynthesis of the molybdenum-dependent cofactor (FeMo-co) equivalent. A number of genes considered to be essential for nitrogen fixation by FeFe nitrogenase, including nifM, vnfEN, and anfOR, are not required for the artificial Anf system in E. coli. This finding has enabled us to engineer a minimal FeFe nitrogenase system comprising the structural anfHDGK genes and the nifBUSV genes required for metallocluster biosynthesis, with nifF and nifJ providing electron transport to the alternative nitrogenase. This minimal Anf system has potential implications for engineering diazotrophy in eukaryotes, particularly in compartments (e.g., organelles) where molybdenum may be limiting. PMID:25139995

  13. Brayton heat exchanger unit development program (alternate design)

    NASA Technical Reports Server (NTRS)

    Duncan, J. D.; Gibson, J. C.; Graves, R. F.; Morse, C. J.; Richard, C. E.

    1973-01-01

    A Brayton Heat Exchanger Unit Alternate Design (BHXU-Alternate) consisting of a recuperator, a heat sink heat exchanger, and a gas ducting system, was designed and fabricated. The design was formulated to provide a high performance unit suitable for use in a long-life Brayton-cycle powerplant. Emphasis was on double containment against external leakage and leakage of the organic coolant into the gas stream. A parametric analysis and design study was performed to establish the optimum component configurations to achieve low weight and size and high reliability, while meeting the requirements of high effectiveness and low pressure drop. Layout studies and detailed mechanical and structural design were performed to obtain a flight-type packaging arrangement, including the close-coupled integration of the BHXU-Alternate with the Brayton Rotating Unit (BRU).

  14. 77 FR 32038 - Energy Conservation Program: Alternative Efficiency Determination Methods and Alternative Rating...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ... the Federal Register on April 18, 2011. 76 FR 21673. The RFI requested suggestions, comments, and... ;dct=FR%252BPR%252BN%252BO%252BSR%252BPS;rpp=25;po=0;D=EER E-2011-BT-TP-0024. Table II.1--Stakeholders... Efficiency Determination Methods and Alternative Rating Methods AGENCY: Office of Energy Efficiency...

  15. ORNL Research Programs related to chlorofluorocarbon (CFC) alternatives. [Chlorofluorocarbons

    SciTech Connect

    Fairchild, P.D.

    1987-05-13

    Testimony covered: uses and control impacts; federal/Department of Energy role; and relevant DOE research and development (absorption cycle technology, advanced insulation, novel thermodynamic cycles, fluid substitutes and recovery technology, development of replacement chemicals, development of recycling and recovery practices, development of alternative technologies, and resource requirements and schedules for accomplishment). (PSB)

  16. The Twilight Academy: An Alternative Education Program that Works

    ERIC Educational Resources Information Center

    D'Angelo, Frank; Zemanick, Robert

    2009-01-01

    Today's adolescent learners are more diverse than ever in terms of their backgrounds, interests, learning styles, and motivations. Thus, school officials must address these differences by thinking outside of the box and creating alternative education settings that acknowledge the fact that not everyone can learn in the traditional classroom…

  17. 76 FR 77716 - Alternate Passenger Rail Service Pilot Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-14

    ... and Development, FRA, 1200 New Jersey Ave. SE., Washington, DC 20590 (telephone: (202) 493-6109); or... proposed rulemaking (NPRM) published on September 7, 2011 (76 FR 55335), FRA proposed an alternate... 44 FR 11034; February 26, 1979. FRA has prepared and placed in the docket a regulatory...

  18. 24 CFR 7.5 - EEO Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Statement regarding Alternative Dispute Resolution (ADR) located on the Department's website and 29 CFR 1614... 29 CFR 1614.105(c). The aggrieved person's election to proceed through ADR instead of EEO counseling... terms, the aggrieved person or Complainant may exercise the right of appeal pursuant to 29 CFR...

  19. 24 CFR 7.5 - EEO Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Statement regarding Alternative Dispute Resolution (ADR) located on the Department's website and 29 CFR 1614... 29 CFR 1614.105(c). The aggrieved person's election to proceed through ADR instead of EEO counseling... terms, the aggrieved person or Complainant may exercise the right of appeal pursuant to 29 CFR...

  20. 24 CFR 7.5 - EEO Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Statement regarding Alternative Dispute Resolution (ADR) located on the Department's website and 29 CFR 1614... 29 CFR 1614.105(c). The aggrieved person's election to proceed through ADR instead of EEO counseling... terms, the aggrieved person or Complainant may exercise the right of appeal pursuant to 29 CFR...

  1. 24 CFR 7.5 - EEO Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Statement regarding Alternative Dispute Resolution (ADR) located on the Department's website and 29 CFR 1614... 29 CFR 1614.105(c). The aggrieved person's election to proceed through ADR instead of EEO counseling... terms, the aggrieved person or Complainant may exercise the right of appeal pursuant to 29 CFR...

  2. Survey and Analysis of Alternative Education Programs II

    ERIC Educational Resources Information Center

    Hosley, Nathaniel S.; Hosley, Jessica; Thein, Myint

    2009-01-01

    This research is a follow up to a similar study published by the Center for Rural Pennsylvania in 2003 in which researchers examined alternative education policies and practices among Pennsylvania school districts. The current study extended the previous research to include information on the perceptions of administrators and teachers on the…

  3. An Alternative Kaposi's Sarcoma-Associated Herpesvirus Replication Program Triggered by Host Cell Apoptosis

    PubMed Central

    Prasad, Alka; Lu, Michael; Lukac, David M.

    2012-01-01

    Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to several neoplastic diseases: Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease (MCD). KSHV replicates actively, via a controlled gene expression program, but can also remain latent. It had been thought that the transition from latent to lytic replication was controlled exclusively by the replication and transcription activator protein RTA (open reading frame 50 [ORF50] gene product). A dominant-negative (DN) ORF50 mutant, ORF50ΔSTAD, blocks gene expression and replication. We produced a PEL cell line derivative containing both latent KSHV genomes and an inducible ORF50ΔSTAD. We unexpectedly found that induction of apoptosis triggered high-level viral replication, even when DN ORF50ΔSTAD was present, suggesting that apoptosis triggers KSHV replication through a distinct RTA-independent pathway. We verified that apoptosis triggers KSHV replication independent of RTA using ORF50 small interfering RNA (siRNA) and also showed that caspase activity is required to trigger KSHV replication. We showed that when apoptosis triggers KSHV replication, the kinetics of late gene expression is accelerated by 12 to 24 h and that virus produced following apoptosis has reduced infectivity. KSHV therefore appears to replicate via two distinct pathways, a conventional pathway requiring RTA, with slower replication kinetics, producing virus with higher infectivity, and an alternative apoptosis-triggered pathway that does not require RTA, has faster replication kinetics, and produces virus with lower infectivity. The existence of a distinct apoptosis-triggered, accelerated replication pathway may have evolutionary advantages for the virus and clinical significance for the treatment of KSHV-associated neoplasms. It also provides further evidence that KSHV can sense and react to its environment. PMID:22345480

  4. Global genome splicing analysis reveals an increased number of alternatively spliced genes with aging.

    PubMed

    Rodríguez, Sofía A; Grochová, Diana; McKenna, Tomás; Borate, Bhavesh; Trivedi, Niraj S; Erdos, Michael R; Eriksson, Maria

    2016-04-01

    Alternative splicing (AS) is a key regulatory mechanism for the development of different tissues; however, not much is known about changes to alternative splicing during aging. Splicing events may become more frequent and widespread genome-wide as tissues age and the splicing machinery stringency decreases. Using skin, skeletal muscle, bone, thymus, and white adipose tissue from wild-type C57BL6/J male mice (4 and 18 months old), we examined the effect of age on splicing by AS analysis of the differential exon usage of the genome. The results identified a considerable number of AS genes in skeletal muscle, thymus, bone, and white adipose tissue between the different age groups (ranging from 27 to 246 AS genes corresponding to 0.3-3.2% of the total number of genes analyzed). For skin, skeletal muscle, and bone, we included a later age group (28 months old) that showed that the number of alternatively spliced genes increased with age in all three tissues (P < 0.01). Analysis of alternatively spliced genes across all tissues by gene ontology and pathway analysis identified 158 genes involved in RNA processing. Additional analysis of AS in a mouse model for the premature aging disease Hutchinson-Gilford progeria syndrome was performed. The results show that expression of the mutant protein, progerin, is associated with an impaired developmental splicing. As progerin accumulates, the number of genes with AS increases compared to in wild-type skin. Our results indicate the existence of a mechanism for increased AS during aging in several tissues, emphasizing that AS has a more important role in the aging process than previously known. PMID:26685868

  5. Development and Delivery of an FCS Alternative Program for License

    ERIC Educational Resources Information Center

    Larson, Marsha L.

    2013-01-01

    When addressing the ongoing shortage of family and consumer sciences (FCS) teachers, it is important to accommodate those unable to follow a traditional college program. This article describes a program that is beneficial to practicing professionals; it utilizes technology to aid in effective communication and efficiency of content delivery.…

  6. An Alternative Feedback/Evaluation Model for Outdoor Wilderness Programs.

    ERIC Educational Resources Information Center

    Dawson, R.

    Project D.A.R.E. (Development through Adventure, Responsibility and Education), an adventure-based outdoor program, uses a feedback/evaluation model, combining a learning component with a two-part participant observational model. The first phase focuses on evaluation of the child and progress made while he is in the program (stages one to four);…

  7. Newcomer Programs: An Educational Alternative for Secondary Immigrant Students.

    ERIC Educational Resources Information Center

    Short, Deborah J.

    2002-01-01

    Examines the small but growing presence of newcomer programs being implemented in urban middle and high schools, providing the rationale for newcomer schools for recent, limited-English-speaking immigrants and summarizing data collected from a national study. Describes program designs in urban schools and their educational goals, instructional…

  8. Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene

    SciTech Connect

    Cybulsky, M.I.; Fries, J.W.U.; Williams, A.J.; Sultan, P.; Gimbrone, M.A. Jr.; Collins, T. ); Eddy, R.; Byers, M.; Shows, T. )

    1991-09-01

    Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Genomic clones encoding the VCAM1 gene were isolated and the organization of the gene was determined. The gene, which is present in a single copy in the human genome, contains 9 exons spanning {approx}25 kilobases of DNA. Exons 2-8 contain C2 or H-type immunoglobulin domains. At least two different VCAM-1 precursors can be generated from the human gene as a result of alternative mRNA splicing events, which include or exclude exon 5. A consensus TATAA element is located upstream of the transcriptional start site. The VCAM1 promoter contains consensus binding sites for NF-{kappa}B, the GATA family of transcription factors, as well as an AP1 site. The VCAM1 gene was assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. Structural analysis of the human VCAM1 gene provides the basis for alternative mRNA splicing and an initial approach to elucidating the regulation of VCAM-1 expression.

  9. Analysis of genes encoding an alternative nitrogenase in the archaeon Methanosarcina barkeri 227.

    PubMed

    Chien, Y T; Auerbuch, V; Brabban, A D; Zinder, S H

    2000-06-01

    Methanosarcina barkeri 227 possesses two clusters of genes potentially encoding nitrogenases. We have previously demonstrated that one cluster, called nif2, is expressed under molybdenum (Mo)-sufficient conditions, and the deduced amino acid sequences for nitrogenase structural genes in that cluster most closely resemble those for the Mo nitrogenase of the gram-positive eubacterium Clostridium pasteurianum. The previously cloned nifH1 from M. barkeri shows phylogenetic relationships with genes encoding components of eubacterial Mo-independent eubacterial alternative nitrogenases and other methanogen nitrogenases. In this study, we cloned and sequenced nifD1 and part of nifK1 from M. barkeri 227. The deduced amino acid sequence encoded by nifD1 from M. barkeri showed great similarity with vnfD gene products from vanadium (V) nitrogenases, with an 80% identity at the amino acid level with the vnfD gene product from Anabaena variabilis. Moreover, there was a small open reading frame located between nifD1 and nifK1 with clear homology to vnfG, a hallmark of eubacterial alternative nitrogenases. Stimulation of diazotrophic growth of M. barkeri 227 by V in the absence of Mo was demonstrated. The unusual complement of nif genes in M. barkeri 227, with one cluster resembling that from a gram-positive eubacterium and the other resembling a eubacterial V nitrogenase gene cluster, suggests horizontal genetic transfer of those genes. PMID:10809706

  10. EPAct Alternative Fuel Transportation Program: State and Alternative Fuel Provider Fleet Compliance Annual Report; Fleet Compliance Results for MY 2013/FY 2014

    SciTech Connect

    2015-09-01

    Compliance rates for covered state government and alternative fuel provider fleets under the Alternative Fuel Transportation Program (pursuant to the Energy Policy Act or EPAct) are reported for MY 2013/FY 2014 in this publication.

  11. Global variability in gene expression and alternative splicing is modulated by mitochondrial content.

    PubMed

    Guantes, Raul; Rastrojo, Alberto; Neves, Ricardo; Lima, Ana; Aguado, Begoña; Iborra, Francisco J

    2015-05-01

    Noise in gene expression is a main determinant of phenotypic variability. Increasing experimental evidence suggests that genome-wide cellular constraints largely contribute to the heterogeneity observed in gene products. It is still unclear, however, which global factors affect gene expression noise and to what extent. Since eukaryotic gene expression is an energy demanding process, differences in the energy budget of each cell could determine gene expression differences. Here, we quantify the contribution of mitochondrial variability (a natural source of ATP variation) to global variability in gene expression. We find that changes in mitochondrial content can account for ∼50% of the variability observed in protein levels. This is the combined result of the effect of mitochondria dosage on transcription and translation apparatus content and activities. Moreover, we find that mitochondrial levels have a large impact on alternative splicing, thus modulating both the abundance and type of mRNAs. A simple mathematical model in which mitochondrial content simultaneously affects transcription rate and splicing site choice can explain the alternative splicing data. The results of this study show that mitochondrial content (and/or probably function) influences mRNA abundance, translation, and alternative splicing, which ultimately affects cellular phenotype. PMID:25800673

  12. Digital-computer program for design analysis of salient, wound pole alternators

    NASA Technical Reports Server (NTRS)

    Repas, D. S.

    1973-01-01

    A digital computer program for analyzing the electromagnetic design of salient, wound pole alternators is presented. The program, which is written in FORTRAN 4, calculates the open-circuit saturation curve, the field-current requirements at rated voltage for various loads and losses, efficiency, reactances, time constants, and weights. The methods used to calculate some of these items are presented or appropriate references are cited. Instructions for using the program and typical program input and output for an alternator design are given, and an alphabetical list of most FORTRAN symbols and the complete program listing with flow charts are included.

  13. FEMP (Federal Energy Management Program) presents alternative financing guidance memoranda

    SciTech Connect

    1998-06-01

    Utility financing of energy efficient measures becomes easier to accomplish with the two new alternative financing guidance memoranda, released April 17, 1998, that address the use of utility incentives for Federal facilities. The memoranda have been approved by the Alternative Financing Guidance Committee on the Interagency Energy Management Task Force. The memoranda include: (1) Policy Statement No. 001: Authority to Sole Source Utility Service Contracts as Referenced in Section 152 of the Energy Policy Act (EPACT) of 1992; and (2) Policy Statement No. 002: Congressional Notification for Utility Projects Under the Authority of Section 152 of the Energy Policy Act (EPACT) of 1992. The purpose for developing the financing memoranda was to address specific issues within current Federal procurement regulations that require clarification or guidance. This new guidance will allow for increased use of utility incentives as a means of financing energy efficient and life cycle cost-effective projects in Federal facilities.

  14. Behavioral Outcomes of an Alternative Program for Junior High School Students at Risk of Dropping Out.

    ERIC Educational Resources Information Center

    Griffin, Edward; And Others

    The study examines the relationship between success upon leaving an alternative school program and immediate measures of program effect. The strength of the relationship was used to determine the degree to which the program effected its long term goals of preparing students for future academic or vocational success. Student success was defined as…

  15. Prevention-Related Research Targeting African American Alternative Education Program Students

    ERIC Educational Resources Information Center

    Carswell, Steven B.; Hanlon, Thomas E.; Watts, Amy M.; O'Grady, Kevin E.

    2014-01-01

    This article reports on a program of research that examined the background, planning, implementation, and evaluation of an after-school preventive intervention program within an ongoing urban alternative education program targeting African American students referred to the school because of their problematic behavior in regular schools. The…

  16. AB 3059: A Report to the Governor on the Alternative Child Care Programs, 1976-80.

    ERIC Educational Resources Information Center

    Hailey, Jack

    This publication of the California Governor's Committee on Child Development Programs focuses on the Alternative Child Care Program in California (AB 3059). The report looks back over four years of the AB 3059, reviews the goals of that legislation, and measures the program's accomplishments against those goals. In early 1976, the Governor's…

  17. 76 FR 64124 - Implementation of the Alternative Dispute Resolution Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-17

    ... INFORMATION: I. Background On September 6, 2011 (76 FR 55136), the NRC announced its intention to hold a... prolonged litigation and unnecessary expenses. The second sub-program (commonly referred to as...

  18. High-fidelity promoter profiling reveals widespread alternative promoter usage and transposon-driven developmental gene expression

    PubMed Central

    Batut, Philippe; Dobin, Alexander; Plessy, Charles; Carninci, Piero; Gingeras, Thomas R.

    2013-01-01

    Many eukaryotic genes possess multiple alternative promoters with distinct expression specificities. Therefore, comprehensively annotating promoters and deciphering their individual regulatory dynamics is critical for gene expression profiling applications and for our understanding of regulatory complexity. We introduce RAMPAGE, a novel promoter activity profiling approach that combines extremely specific 5′-complete cDNA sequencing with an integrated data analysis workflow, to address the limitations of current techniques. RAMPAGE features a streamlined protocol for fast and easy generation of highly multiplexed sequencing libraries, offers very high transcription start site specificity, generates accurate and reproducible promoter expression measurements, and yields extensive transcript connectivity information through paired-end cDNA sequencing. We used RAMPAGE in a genome-wide study of promoter activity throughout 36 stages of the life cycle of Drosophila melanogaster, and describe here a comprehensive data set that represents the first available developmental time-course of promoter usage. We found that >40% of developmentally expressed genes have at least two promoters and that alternative promoters generally implement distinct regulatory programs. Transposable elements, long proposed to play a central role in the evolution of their host genomes through their ability to regulate gene expression, contribute at least 1300 promoters shaping the developmental transcriptome of D. melanogaster. Hundreds of these promoters drive the expression of annotated genes, and transposons often impart their own expression specificity upon the genes they regulate. These observations provide support for the theory that transposons may drive regulatory innovation through the distribution of stereotyped cis-regulatory modules throughout their host genomes. PMID:22936248

  19. Alternate bearing design fabrication and test program, exhibit B

    NASA Technical Reports Server (NTRS)

    1978-01-01

    A 50 ft-lb-sec (FPS) reaction wheel was modified with an ironless armature brushless dc motor and a magnetic bearing suspension. The purpose was to demonstrate the performance of an alternate bearing concept, i.e., a magnetic bearing suspension, which could be used in Skylab type CMG's to meet the attitude control requirements of long term space stations. A magnetic suspension was built, installed and tested in the 50 FPS reaction wheel. A secondary effort included the build and test of a compatible reaction wheel motor. Performance characteristics of both are presented and discussed.

  20. SELENIUM TREATMENT/REMOVAL ALTERNATIVES DEMONSTRATION PROJECT - MINE WASTE TECHNOLOGY PROGRAM ACTIVITY III, PROJECT 20

    EPA Science Inventory

    This document is the final report for EPA's Mine WAste Technology Program (MWTP) Activity III, Project 20--Selenium Treatment/Removal Alternatives Demonstration project. Selenium contamination originates from many sources including mining operations, mineral processing, abandoned...

  1. 78 FR 45253 - National Toxicology Program Scientific Advisory Committee on Alternative Toxicological Methods...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-26

    ... HUMAN SERVICES National Institutes of Health National Toxicology Program Scientific Advisory Committee... notice announces a meeting of the Scientific Advisory Committee on Alternative Toxicological Methods... and promotes the scientific validation and regulatory acceptance of toxicological and...

  2. 77 FR 14587 - FY 2012 Discretionary Livability Funding Opportunity: Alternatives Analysis Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-12

    ... Federal Transit Administration FY 2012 Discretionary Livability Funding Opportunity: Alternatives Analysis Program AGENCY: Federal Transit Administration (FTA), DOT. ACTION: Notice of funding availability for FTA... by the Temporary Authorization. This notice solicits proposals to compete for FY 2012 funding...

  3. 5 CFR 2424.10 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Collaboration and Alternative Dispute... Collaboration and Alternative Dispute Resolution Program. Where an exclusive representative and an agency are unable to resolve disputes that arise under this part, they may request assistance from the...

  4. 5 CFR 2424.10 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Collaboration and Alternative Dispute... Collaboration and Alternative Dispute Resolution Program. Where an exclusive representative and an agency are unable to resolve disputes that arise under this part, they may request assistance from the...

  5. 5 CFR 2424.10 - Collaboration and Alternative Dispute Resolution Program.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Collaboration and Alternative Dispute... Collaboration and Alternative Dispute Resolution Program. Where an exclusive representative and an agency are unable to resolve disputes that arise under this part, they may request assistance from the...

  6. Alternative Programs for At-Risk Students: Wolves in Sheep's Clothing?

    ERIC Educational Resources Information Center

    Sagor, Richard

    1997-01-01

    Provides a critique of alternative education and details the disadvantages of segregated schooling. Discusses the problems associated with labeling children as "problem students" and outlines ways in which equitable alternative programs can be devised. Gives a case study as an example. (RJM)

  7. 46 CFR 115.660 - Continued participation in the Alternative Hull Examination (AHE) Program.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Continued participation in the Alternative Hull... MORE THAN 49 PASSENGERS INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 115.660 Continued participation in the Alternative Hull Examination (AHE) Program. (a) To continue to participate...

  8. 46 CFR 115.660 - Continued participation in the Alternative Hull Examination (AHE) Program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Continued participation in the Alternative Hull... MORE THAN 49 PASSENGERS INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 115.660 Continued participation in the Alternative Hull Examination (AHE) Program. (a) To continue to participate...

  9. 46 CFR 176.660 - Continued participation in the Alternative Hull Examination (AHE) Program.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Continued participation in the Alternative Hull... (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 176.660 Continued participation in the Alternative Hull Examination (AHE) Program. (a)...

  10. 46 CFR 176.660 - Continued participation in the Alternative Hull Examination (AHE) Program.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Continued participation in the Alternative Hull... (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 176.660 Continued participation in the Alternative Hull Examination (AHE) Program. (a)...

  11. 46 CFR 176.660 - Continued participation in the Alternative Hull Examination (AHE) Program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Continued participation in the Alternative Hull... (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 176.660 Continued participation in the Alternative Hull Examination (AHE) Program. (a)...

  12. 46 CFR 176.660 - Continued participation in the Alternative Hull Examination (AHE) Program.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Continued participation in the Alternative Hull... (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 176.660 Continued participation in the Alternative Hull Examination (AHE) Program. (a)...

  13. 46 CFR 115.660 - Continued participation in the Alternative Hull Examination (AHE) Program.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Continued participation in the Alternative Hull... MORE THAN 49 PASSENGERS INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 115.660 Continued participation in the Alternative Hull Examination (AHE) Program. (a) To continue to participate...

  14. 46 CFR 115.660 - Continued participation in the Alternative Hull Examination (AHE) Program.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Continued participation in the Alternative Hull... MORE THAN 49 PASSENGERS INSPECTION AND CERTIFICATION Hull and Tailshaft Examinations § 115.660 Continued participation in the Alternative Hull Examination (AHE) Program. (a) To continue to participate...

  15. State Approved Alternative Certification: Are These Programs Changing the Face of Teacher Preparation?

    ERIC Educational Resources Information Center

    James, Terry; McNiece, Elaine

    The paper reviews a national survey of alternative teacher certification programs, comparing the data to: (1) Teacher Education Policy in the States: A 50-State Survey of Legislative and Administrative Actions (American Association of Colleges for Teacher Education (AACTE), December 1990); and (2) Alternative Teacher Certification: A…

  16. Inclusion of Alternative and Complementary Therapies in CACREP Training Programs: A Survey

    ERIC Educational Resources Information Center

    Lumadue, Christine A.; Munk, Melanie; Wooten, H. Ray

    2005-01-01

    Given a heightened focus within the mental health profession on creative, complementary, and alternative practices, the authors surveyed CACREP programs with respect to their inclusion of such approaches in counselor training. For the purpose of this study, these approaches were designated as complementary and alternative methods (CAM) and defined…

  17. Alternate space shuttle concepts study: Design requirements and phased programs evaluation

    NASA Technical Reports Server (NTRS)

    1971-01-01

    A study to determine program and technical alternatives to the design of the space shuttle orbiter is described. The alternatives include a phased approach, involving orbiter development and operation with an expendable booster for an interim period, as well as design variations to the basic vehicle. The space shuttle orbiter configurations and predicted performance parameters are presented.

  18. Alternative Break Programs: From Isolated Enthusiasm to Best Practices--The Haiti Compact

    ERIC Educational Resources Information Center

    Piacitelli, Jill; Barwick, Molly; Doerr, Elizabeth; Porter, Melody; Sumka, Shoshanna

    2013-01-01

    Alternative break programs, which are short-term service-learning trips, immerse students in direct service and education, resulting in the creation of active citizens who think and act critically around the root causes of social issues. Over the last 20 years, domestic alternative breaks have effectively created strong community partnerships and…

  19. Experiments in Alternative Energy Program Design: Final Report, Volume One

    SciTech Connect

    Farhar, B. C.

    1989-04-01

    The Solar Energy Research Institute issued a Notice of Program Interest (NOPI) which resulted in the selection of six projects. The balance of this report briefly describes each of the six projects in turn, and then evaluates the NOPI process, which was in itself an experiment.

  20. Modular Laboratory Courses: An Alternative to a Traditional Laboratory Program

    ERIC Educational Resources Information Center

    Caprette, David R.; Armstrong, Sarah; Beason, K. Beth

    2005-01-01

    Our modular laboratory teaching program is characterized by two major features. First, each course is taught independently and not linked with a particular lecture course. Second, each course is designed to be completed within one-half semester or less. The modular organization has allowed incorporation of the latest technology, reduction of class…

  1. Clarendon Alternative School Japanese Bilingual Bicultural Program: Curriculum Sampler.

    ERIC Educational Resources Information Center

    San Francisco Unified School District, CA.

    Sample lessons and instructional materials from a Japanese bilingual/bicultural elementary school program are presented. The lessons are designed to integrate Japanese language instruction with content instruction, using thematic units related to the core curriculum. The ten lessons are organized by target grade (K-5), and describe classroom…

  2. Pacific area wide program for methyl bromide alternatives – Ornamentals

    Technology Transfer Automated Retrieval System (TEKTRAN)

    During the past 5 years the following field trials have been conducted as part of the Pacific Area-Wide program for cut flowers and ornamentals using various combinations of 1, 3-dichloropropene, chloropicrin, and metam: 1) Drip demonstration trial with field-grown calla lilies at Golden State Bulb ...

  3. Effects of hydrogen sulfide on alternative pathway respiration and induction of alternative oxidase gene expression in rice suspension cells.

    PubMed

    Xiao, Man; Ma, Jun; Li, Hongyu; Jin, Han; Feng, Hanqing

    2010-01-01

    The toxic effects of H2S on plants are well documented. However, the molecular mechanisms reponsible for inhibition of plants by H2S are still not completely understood. We determined the effects of NaHS in the range of 0.5-10 mM on the growth of rice suspension culture cells, as well as on the expression of the alternative oxidase (AOX) gene. AOX is the terminal oxidase of the alternative pathway (AP) and exists in plant mitochondria. The results showed that H2S treatment enhanced the AP activity. During the process of H2S treatment for 4 h, the AP activity increased dramatically and achieved the peak value at a concentration of 2 mM NaHS. Then it declined at higher concentrations of NaHS (5-10 mM) and maintained a steady level. The AOX1 gene transcript level also showed a similar change as the AP activity. Interestingly, different NaHS concentrations seemed to have different effects on the expression of AOX1a, AOX1b, and AOX1c. The induction of AOX expression by low concentrations of NaHS was inferred through a reactive oxygen species (ROS)-independent pathway. At the same time, rice cells grown in culture were very sensitive to H2S, different H2S concentrations induced an increase in the cell viability. These results indicate that the H2S-induced AOX induction might play a role in inhibiting the ROS production and have an influence on cell viability. PMID:20737915

  4. Update from the NREL Alternative Fuel Transit Bus Evaluation Program

    SciTech Connect

    Chandler, K.; Norton, P.; Clark, N.

    1999-05-01

    The object of this project, which is supported by the U.S. Department of Energy (DOE) through the National Renewable Energy Laboratory (NREL), is to provide a comprehensive comparison of heavy-duty urban transit buses operating on alternative fuels and diesel fuel. Final reports from this project were produced in 1996 from data collection and evaluation of 11 transit buses from eight transit sites. With the publication of these final reports, three issues were raised that needed further investigation: (1) the natural gas engines studied were older, open-loop control engines; (2) propane was not included in the original study; and (3) liquefied natural gas (LNG) was found to be in the early stages of deployment in transit applications. In response to these three issues, the project has continued by emissions testing newer natural gas engines and adding two new data collection sites to study the newer natural gas technology and specifically to measure new technology LNG buses.

  5. A Collaborative Alternative Path Program for Career-Changing Mathematics and Science Professionals: Context, Design, and Replication

    ERIC Educational Resources Information Center

    Selke, Mary J.; Fero, George J.

    2005-01-01

    The purpose of this article is to provide insights useful to readers involved in or considering the design and delivery of alternative path programs by presenting (a) a chronology of the multiple definitions of alternative certification, (b) key components of quality alternative path programs, viewed through the example of a program for…

  6. Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies

    PubMed Central

    2010-01-01

    Background Quantitative real-time RT-PCR (RT-qPCR) is a highly sensitive method for mRNA quantification, but requires invariant expression of the chosen reference gene(s). In pathological myocardium, there is limited information on suitable reference genes other than the commonly used Gapdh mRNA and 18S ribosomal RNA. Our aim was to evaluate and identify suitable reference genes in human failing myocardium, in rat and mouse post-myocardial infarction (post-MI) heart failure and across developmental stages in fetal and neonatal rat myocardium. Results The abundance of Arbp, Rpl32, Rpl4, Tbp, Polr2a, Hprt1, Pgk1, Ppia and Gapdh mRNA and 18S ribosomal RNA in myocardial samples was quantified by RT-qPCR. The expression variability of these transcripts was evaluated by the geNorm and Normfinder algorithms and by a variance component analysis method. Biological variability was a greater contributor to sample variability than either repeated reverse transcription or PCR reactions. Conclusions The most stable reference genes were Rpl32, Gapdh and Polr2a in mouse post-infarction heart failure, Polr2a, Rpl32 and Tbp in rat post-infarction heart failure and Rpl32 and Pgk1 in human heart failure (ischemic disease and cardiomyopathy). The overall most stable reference genes across all three species was Rpl32 and Polr2a. In rat myocardium, all reference genes tested showed substantial variation with developmental stage, with Rpl4 as was most stable among the tested genes. PMID:20331858

  7. Multidimensional Programming Methods for Energy Facility Siting: Alternative Approaches

    NASA Technical Reports Server (NTRS)

    Solomon, B. D.; Haynes, K. E.

    1982-01-01

    The use of multidimensional optimization methods in solving power plant siting problems, which are characterized by several conflicting, noncommensurable objectives is addressed. After a discussion of data requirements and exclusionary site screening methods for bounding the decision space, classes of multiobjective and goal programming models are discussed in the context of finite site selection. Advantages and limitations of these approaches are highlighted and the linkage of multidimensional methods with the subjective, behavioral components of the power plant siting process is emphasized.

  8. Computer programs for the characterization of protein coding genes.

    PubMed

    Pierno, G; Barni, N; Candurro, M; Cipollaro, M; Franzè, A; Juliano, L; Macchiato, M F; Mastrocinque, G; Moscatelli, C; Scarlato, V

    1984-01-11

    Computer programs, implemented on an Univac II00/80 computer system, for the identification and characterization of protein coding genes and for the analysis of nucleic acid sequences, are described. PMID:6546420

  9. Computer programs for the characterization of protein coding genes.

    PubMed Central

    Pierno, G; Barni, N; Candurro, M; Cipollaro, M; Franzè, A; Juliano, L; Macchiato, M F; Mastrocinque, G; Moscatelli, C; Scarlato, V

    1984-01-01

    Computer programs, implemented on an Univac II00/80 computer system, for the identification and characterization of protein coding genes and for the analysis of nucleic acid sequences, are described. PMID:6546420

  10. Stress-induced alternative gene splicing in mind-body medicine.

    PubMed

    Rossi, Ernest Lawrence

    2004-01-01

    Recent research documents how psychosocial stress can alter the expression of the acetylcholinesterase gene to generate at least 3 alternative proteins that are implicated in a wide variety of normal mind-body functions, as well as pathologies. These range from early embryological development, plasticity of the brain in adulthood, post-traumatic stress disorder (PTSD), and stress-associated dysfunctions of the central nervous, endocrine, and immune systems, to age-related neuropathologies. Such stress-induced alternative gene splicing is proposed here as a major mind-body pathway of psychosocial genomics-the modulation of gene expression by creative psychological, social, and cultural processes. We explore the types of research that are now needed to investigate how stress-induced alternative splicing of the acetylcholinesterase gene may play a pivotal role in the deep psychobiology of psychotherapy, meditation, spiritual rituals, and the experiencing of positive humanistic values that have been associated with mind-body medicine, such as compassion, beneficence, serenity, forgiveness, and gratitude. PMID:15356952