Science.gov

Sample records for annual publication ngs

  1. Biodiversity Monitoring Using NGS Approaches on Unusual Substrates (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect

    Gilbert, Tom

    2013-03-01

    Tom Gilbert of the Natural History Museum of Denmark on "Biodiversity monitoring using NGS approaches on unusual substrates" at the 8th Annual Genomics of Energy & Environment Meeting in Walnut Creek, Calif.

  2. NGS for the Masses: Empowering Biologists to Improve Bioinformatics Productivity ( 7th Annual SFAF Meeting, 2012)

    SciTech Connect

    Qaadri, Kashef

    2012-06-01

    Kashef Qaadri on "NGS for the Masses: Empowering biologists to improve bioinformatic productivity" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

  3. NGS for the Masses: Empowering Biologists to Improve Bioinformatics Productivity ( 7th Annual SFAF Meeting, 2012)

    ScienceCinema

    Qaadri, Kashef [Biomatters

    2013-03-22

    Kashef Qaadri on "NGS for the Masses: Empowering biologists to improve bioinformatic productivity" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

  4. A framework for organizing cancer-related variations from existing databases, publications and NGS data using a High-performance Integrated Virtual Environment (HIVE).

    PubMed

    Wu, Tsung-Jung; Shamsaddini, Amirhossein; Pan, Yang; Smith, Krista; Crichton, Daniel J; Simonyan, Vahan; Mazumder, Raja

    2014-01-01

    Years of sequence feature curation by UniProtKB/Swiss-Prot, PIR-PSD, NCBI-CDD, RefSeq and other database biocurators has led to a rich repository of information on functional sites of genes and proteins. This information along with variation-related annotation can be used to scan human short sequence reads from next-generation sequencing (NGS) pipelines for presence of non-synonymous single-nucleotide variations (nsSNVs) that affect functional sites. This and similar workflows are becoming more important because thousands of NGS data sets are being made available through projects such as The Cancer Genome Atlas (TCGA), and researchers want to evaluate their biomarkers in genomic data. BioMuta, an integrated sequence feature database, provides a framework for automated and manual curation and integration of cancer-related sequence features so that they can be used in NGS analysis pipelines. Sequence feature information in BioMuta is collected from the Catalogue of Somatic Mutations in Cancer (COSMIC), ClinVar, UniProtKB and through biocuration of information available from publications. Additionally, nsSNVs identified through automated analysis of NGS data from TCGA are also included in the database. Because of the petabytes of data and information present in NGS primary repositories, a platform HIVE (High-performance Integrated Virtual Environment) for storing, analyzing, computing and curating NGS data and associated metadata has been developed. Using HIVE, 31 979 nsSNVs were identified in TCGA-derived NGS data from breast cancer patients. All variations identified through this process are stored in a Curated Short Read archive, and the nsSNVs from the tumor samples are included in BioMuta. Currently, BioMuta has 26 cancer types with 13 896 small-scale and 308 986 large-scale study-derived variations. Integration of variation data allows identifications of novel or common nsSNVs that can be prioritized in validation studies. Database URL: BioMuta: http

  5. A framework for organizing cancer-related variations from existing databases, publications and NGS data using a High-performance Integrated Virtual Environment (HIVE)

    PubMed Central

    Wu, Tsung-Jung; Shamsaddini, Amirhossein; Pan, Yang; Smith, Krista; Crichton, Daniel J.; Simonyan, Vahan; Mazumder, Raja

    2014-01-01

    Years of sequence feature curation by UniProtKB/Swiss-Prot, PIR-PSD, NCBI-CDD, RefSeq and other database biocurators has led to a rich repository of information on functional sites of genes and proteins. This information along with variation-related annotation can be used to scan human short sequence reads from next-generation sequencing (NGS) pipelines for presence of non-synonymous single-nucleotide variations (nsSNVs) that affect functional sites. This and similar workflows are becoming more important because thousands of NGS data sets are being made available through projects such as The Cancer Genome Atlas (TCGA), and researchers want to evaluate their biomarkers in genomic data. BioMuta, an integrated sequence feature database, provides a framework for automated and manual curation and integration of cancer-related sequence features so that they can be used in NGS analysis pipelines. Sequence feature information in BioMuta is collected from the Catalogue of Somatic Mutations in Cancer (COSMIC), ClinVar, UniProtKB and through biocuration of information available from publications. Additionally, nsSNVs identified through automated analysis of NGS data from TCGA are also included in the database. Because of the petabytes of data and information present in NGS primary repositories, a platform HIVE (High-performance Integrated Virtual Environment) for storing, analyzing, computing and curating NGS data and associated metadata has been developed. Using HIVE, 31 979 nsSNVs were identified in TCGA-derived NGS data from breast cancer patients. All variations identified through this process are stored in a Curated Short Read archive, and the nsSNVs from the tumor samples are included in BioMuta. Currently, BioMuta has 26 cancer types with 13 896 small-scale and 308 986 large-scale study-derived variations. Integration of variation data allows identifications of novel or common nsSNVs that can be prioritized in validation studies. Database URL: BioMuta: http

  6. Alabama Public Library Service: 1998 Annual Report.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    The Alabama Public Library Service (APLS) is responsible for receiving and administering federal and state funds for the more than 200 public libraries in Alabama. This document represents the annual report for the Alabama Public Library Service for fiscal year 1998. Information is reported under the following categories: Evaluation and Research;…

  7. Alabama Public Library Service. Annual Report, 1979.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    This annual report highlights services to the blind and physically handicapped, progress in library automation and interlibrary cooperation, administration of state aid and funding from the Library Services and Construction Act, library development-planning and research, public library income, public information, continuing education, and other…

  8. Alabama Public Library Service Annual Report.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    The Alabama Public Library Service (APLS) lists its duties, goals, and recent accomplishments in this 1988 annual report. Some of these duties and goals are: (1) administering grants for library development and state aid; (2) providing consultation for library automation; (3) researching and answering reference questions; (4) lending books from…

  9. Alabama Public Library Service, 1987 Annual Report.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    Designed to provide an overview of the range and quality of services provided by the Alabama Public Library Service (APLS), this annual report focuses on the 1987 activities of APLS. A report on the activities of the Library Development Division shows the allocation of state aid and Library Services and Construction Act (LCSA) Titles I and III…

  10. Alabama Public Library Service Annual Report, 1990.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    This annual report highlights the accomplishments of the Alabama Public Library Service (APLS) during 1990. Introductory materials include a map of Alabama's Congressional districts, photographs of the executive board of APLS, and comments from the chairman of the executive board and from APLS' director. Accomplishments are reported under the…

  11. Alabama Public Library Service Annual Report, 1989.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    This annual report highlights the accomplishments of the Alabama Public Library Service (APLS) during 1989. Introductory materials include a map of Alabama's Congressional districts, photographs of the executive board of APLS, and comments from the chairman of the executive board and from APLS' director. The following accomplishments are then…

  12. Houston Public Libraries Annual Report, 1977.

    ERIC Educational Resources Information Center

    Houston Public Library, TX.

    Primary focus in this 1977 annual report of the Houston Public Library system is the library's nine-month program, "City...Our Urban Past, Present and Future," which was funded by a grant from the National Endowment for the Humanities. Aspects of the first four months of the program that are described include its lecture series, exhibits, reading…

  13. 75 FR 9427 - Notice of Proposed Information Collection for Public Comment; Public Housing Annual Contributions...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection for Public Comment; Public Housing Annual Contributions Contract AGENCY: Office of the Assistant Secretary for Public and Indian Housing, HUD. ACTION... lists the following information: Title of Proposal: Public Housing Annual Contributions Contract....

  14. 75 FR 39034 - Public Housing Annual Contributions Contract

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-07

    ... URBAN DEVELOPMENT Public Housing Annual Contributions Contract AGENCY: Office of the Chief Information... conditions contained in an Annual Contributions Contract (ACC) with certain requirements applicable to all... electronic submission of responses. This notice also lists the following information: Title of...

  15. 78 FR 5516 - Sunshine Act: OPIC Annual Public Hearing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-25

    ... CORPORATION Sunshine Act: OPIC Annual Public Hearing TIME AND DATE: 2 p.m., Wednesday, March 13, 2013. PLACE...: Hearing open to the public at 2 p.m. PURPOSE: Annual Public Hearing to afford an opportunity for any... INFORMATION: OPIC is a U.S. Government agency that provides, on a commercial basis, political risk...

  16. 75 FR 76758 - Sunshine Act; OPIC Annual Public Hearing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-09

    ... CORPORATION Sunshine Act; OPIC Annual Public Hearing Time and Date: 3:30 p.m., Thursday, January 20, 2011.... Status: Hearing OPEN to the Public at 3:30 p.m. Purpose: Annual Public Hearing to afford an opportunity...: OIC is a U.S. Government agency that provides, on a commercial basis, political risk insurance...

  17. 77 FR 43618 - Sunshine Act Meetings; OPIC Annual Public Hearing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-25

    ... CORPORATION Sunshine Act Meetings; OPIC Annual Public Hearing TIME AND DATE: 2:00 p.m., Thursday, September 6..., DC. STATUS: Hearing OPEN to the Public at 2:00 p.m. PURPOSE: Annual Public Hearing to afford an... INFORMATION: OPIC is a U.S. Government agency that provides, on a commercial basis, political risk...

  18. Corporation for Public Broadcasting Annual Report, 1971.

    ERIC Educational Resources Information Center

    Corporation for Public Broadcasting, Washington, DC.

    The Corporation for Public Broadcasting (CPB) continued to grow in fiscal year 1971, its third year of operation. Fourteen new public television stations began broadcasting, bringing 7.9 million more persons within receiving range, and bringing the total number of TV stations to 207. Income increased 18 per cent to $14 million due largely to more…

  19. Alabama Public Library Service Annual Report, 1999.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    The Alabama Public Library Service (APLS) is charged with improving library services throughout the state to ensure that all citizens have access to quality library and information services. The agency is responsible for receiving and administering federal and state funds for the more than 200 public libraries in Alabama. Information is reported…

  20. Alabama Public Library Service: 1997 Annual Report.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    The Alabama Public Library Service (APLS) serves the information needs of Alabama public libraries. As a state agency, APLS is charged with improving library services throughout the state to ensure that all citizens have access to quality library and information services. The agency is responsible for receiving and administering federal and state…

  1. Alabama Public Library Service 1996 Annual Report.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    The Alabama Public Library Service is charged with improving library services throughout Alabama to ensure that all citizens have access to quality library and information services. The agency is responsible for administering federal and state funds for the more than 200 public libraries in Alabama. Building renovations were completed in April…

  2. Airborne Gravity Data Enhances NGS Experimental Gravimetric Geoid in Alaska

    NASA Astrophysics Data System (ADS)

    Holmes, S. A.; Childers, V. A.; Li, X.; Roman, D. R.

    2014-12-01

    The U.S. National Geodetic Survey [NGS], through their Gravity for the Redefinition of the American Vertical Datum [GRAV-D] program, continues to update its gravimetry holdings by flying new airborne gravity surveys over a large fraction of the USA and its territories. By 2022, NGS intends that all orthometric heights in the USA will be determined in the field by using a reliable national gravimetric geoid model to transform from geodetic heights obtained from GPS. Several airborne campaigns have already been flown over Alaska and its coastline. Some of this Alaskan coastal data have been incorporated into a new NGS experimental geoid model - xGEOID14. The xGEOID14 model is the first in a series of annual experimental geoid models that will incorporate NGS GRAV-D airborne data. This series provides a useful benchmark for assessing and improving current techniques by which the airborne and land-survey data are filtered and cleaned, and then combined with satellite gravity models, elevation data (etc.) with the ultimate aim of computing a geoid model that can support a national physical height system by 2022. Here we will examine the NGS GRAV-D airborne data in Alaska, and assess its contribution to xGEOID14. Future prospects for xGEOID15 will also be considered.

  3. 77 FR 4568 - Annual Computational Science Symposium; Public Conference

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-30

    ... Drug Administration (FDA), in cosponsorship with the Pharmaceutical Users Software Exchange (PhUSE), is announcing a public conference entitled ``The FDA/PhUSE Annual Computational Science Symposium.'' The purpose...-5300. Contact: Chris Decker, U.S. Regional Director, Pharmaceutical Users Software Exchange (PhUSE),...

  4. State of Alabama: Alabama Public Library Service. Annual Report, 1977.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    Presented in a new format which makes possible statistical analysis and comparison, this annual report includes summaries of special services, as well as statistical reports and studies on public and regional libraries, and two library directories. Special services include long range programs, state aid, the Library Services and Construction Act,…

  5. The Condition of Connecticut's Public School Facilities. Annual Report.

    ERIC Educational Resources Information Center

    Connecticut State Dept. of Education, Hartford.

    This is the second annual report on the condition of Connecticut's public school facilities required pursuant to Section 10-220 of the Connecticut General Statutes. The data in this report reflect the responses of school district officials to various survey questions. The report includes tables which list school-by-school responses to all survey…

  6. Annual Report on the Condition of Connecticut's Public Schools, 2002.

    ERIC Educational Resources Information Center

    Connecticut State Dept. of Education, Hartford.

    This is the fourth annual report on the condition of Connecticut's public school facilities required pursuant to Section 10-220 of the Connecticut General Statutes. The data in this report reflect the responses of school district officials to various survey questions. Summary data are discussed in fairly broad categories--education reference…

  7. Annual Report on the Condition of Connecticut's Public Schools.

    ERIC Educational Resources Information Center

    Connecticut State Dept. of Education, Hartford.

    This is the third annual report on the condition of Connecticut's public school facilities required pursuant to Section 10-220 of the Connecticut General Statutes. The data in this report reflect the responses of school district officials to various survey questions. Summary data are discussed in fairly broad categories--education reference groups…

  8. The 41st Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    ERIC Educational Resources Information Center

    Bushaw, William J.; McNee, John A.

    2009-01-01

    The authors report the findings of the 41st Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools. The annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools is unique because it's not an advocacy poll, but rather a thoughtful study of Americans' current perceptions of their public schools. Each…

  9. What impact do annual public health reports have?

    PubMed

    Fulop, N; McKee, M

    1996-09-01

    Each district health authority is required to produce an annual report on the health of their population. Although they consume considerable resources, there is little evidence as to their impact on their intended audiences. We describe the results of a postal survey of how these reports are viewed by health authority and local government managers in one region. Eighty-nine out of 139 responded (64%). Over half of the managers had read the reports in their entirety. Most were discussed by health authorities but few by relevant local government committees. There was some disagreement between directors of public health and other health authority managers about the impact of the reports on commissioning. The paper discusses the difficulties in evaluating the impact of annual public health reports. The survey reveals a fundamental disagreement about whether the intended audience should be health care purchasers or the public. This reflects an underlying tension about the role of public health and the debate about its independence. PMID:8885669

  10. 86th Annual Georgia Public Health Association Meeting & Conference Report

    PubMed Central

    Smith, Selina A.; Abbott, Regina; Sims, Christy

    2015-01-01

    EXECUTIVE SUMMARY The 86th annual meeting of the Georgia Public Health Association (GPHA) and joint conference with the Southern Health Association was held in Atlanta, Georgia, on April 13–14, 2015, with pre-conference (April 12th) and post-conference (April 14th) Executive Board meetings. As Georgia’s leading forum for public health researchers, practitioners, and students, the annual meeting of the GPHA brings together participants from across the state to explore recent developments in the field and to exchange techniques, tools, and experiences. Historically, the GPHA conference has been held in Savannah (n=24); Jekyll Island (n=20); Atlanta (n=16); Augusta (n=4); and Gainesville (n=1). There was no annual meeting during the early years (1929–1936); during World War II (1941–1943 and 1945); and for four years during the 1980s. Between 2006 and 2010, GPHA held one-day annual meetings and business sessions with educational workshops. Several new initiatives were highlighted as part of this year’s conference. These included a “move and groove” physical activity lounge, registration scholarships for students with a dedicated meet-and-greet reception, an expanded exhibit hall, presentation and approval of three resolutions (related to healthy foods at official activities and events; weapons at official activities and events; and memorials), and approval of the 2015 legislative policy positions and amended association bylaws. The theme for the conference was Advocacy in Action for Public Health. Specifically, the program addressed ensuring access to care; protecting funding for core programs, services, and infrastructure; eliminating health disparities; and addressing key public health issues important to the state of Georgia. One hundred and nine (109) abstracts were submitted for peer review; 36 were accepted for poster and 40 for workshop presentations. Four plenary sessions with keynote speakers covered the intersection between advocacy and policy

  11. 78 FR 57378 - Proposed Information Collection Request; Comment Request; Annual Public Water System Compliance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-18

    ... AGENCY Proposed Information Collection Request; Comment Request; Annual Public Water System Compliance... Protection Agency is planning to submit an information collection request (ICR), ``Annual Public Water System... Public Water Systems Compliance Report; ICR Numbers: EPA ICR Number 1812.05, OMB Control Number...

  12. Oak Ridge Reservation Public Warning Siren System Annual Test Report

    SciTech Connect

    R. F. Gee

    2000-10-01

    The full operational test of the Oak Ridge Reservation (ORR) Public Warning Siren System (PWSS) was successfully conducted on September 27, 2000. The annual test is a full-scale sounding of the individual siren systems around each of the three Department of Energy (DOE) sites in Oak Ridge, Tennessee. The purpose of the annual test is to demonstrate and validate the siren systems' ability to alert personnel outdoors in the Immediate Notification Zones (INZ) (approximately two miles) around each site. The success of this test is based on two critical functions of the siren system. The first function is system operability. The system is considered operable if 90% of the sirens are operational. System diagnostics and direct field observations were used to validate the operability of the siren systems. Based on the diagnostic results and field observations, greater than 90% of the sirens were considered operational. The second function is system audibility. The system is considered audible if the siren could be heard in the immediate notification zones around each of the three sites. Direct field observations, along with sound level measurements, were used to validate the audibility of the siren system. Based on the direct field observations and sound level measurements, the siren system was considered audible. The combination of field observations, system diagnostic status reports, and sound level measurements provided a high level of confidence that the system met and would meet operational requirements upon demand. As part of the overall system test, the Tennessee Emergency Management Agency (TEMA) activated the Emergency Alerting System (EAS), which utilized area radio stations to make announcements regarding the test and to remind residents of what to do in the event of an actual emergency.

  13. The 38th Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    ERIC Educational Resources Information Center

    Rose, Lowell C.; Gallup, Alec M.

    2006-01-01

    Rose and Gallup report on the results of the 38th Annual PDK/Gallup Poll of the Public's Attitude Toward the Public Schools. This year's survey examined No Child Left Behind and the public's perception of the law, the appropriate role of standardized testing, the achievement gap between white students and black and Hispanic students, the…

  14. The 39th Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    ERIC Educational Resources Information Center

    Rose, Lowell C.; Gallup, Alec M.

    2007-01-01

    In this article, the authors report the results of the 39th Annual Phi Delta Kappa/Gallup Poll of the public's attitudes toward the public schools. This year's report examined the public's assessment of the No Child Left Behind (NCLB) and its principal strategy, standardized testing. The authors include a summary of key findings and tables showing…

  15. 76 FR 17657 - Medical Device Epidemiology Network 2011: Second Annual Public Workshop

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-30

    ... HUMAN SERVICES Food and Drug Administration Medical Device Epidemiology Network 2011: Second Annual... Network (MDEpiNet) 2011: Second Annual Public Workshop.'' The purpose of the public workshop is to provide... establishment of a network that works with FDA experts to determine the evidence gaps and questions,...

  16. 77 FR 53935 - Sunshine Act Cancellation Notice-OPIC's September 6, 2012 Annual Public Hearing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    ... From the Federal Register Online via the Government Publishing Office OVERSEAS PRIVATE INVESTMENT CORPORATION Sunshine Act Cancellation Notice--OPIC's September 6, 2012 Annual Public Hearing OPIC's Sunshine Act notice of its Annual Public Hearing was published in the Federal Register (Volume 77, Number...

  17. 78 FR 13912 - Sunshine Act; Cancellation Notice of Annual Public Hearing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ... From the Federal Register Online via the Government Publishing Office OVERSEAS PRIVATE INVESTMENT CORPORATION Sunshine Act; Cancellation Notice of Annual Public Hearing OPIC's Sunshine Act notice of its Annual Public Hearing was published in the Federal Register (Volume 78, Number 17, Page 5516) on...

  18. 40 CFR 256.61 - Requirements for public participation in the annual State work program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES GUIDELINES FOR DEVELOPMENT AND IMPLEMENTATION OF STATE SOLID WASTE MANAGEMENT PLANS Public Participation § 256.61 Requirements for public participation in the annual State work program. (a) A public participation work plan in accord with 40 CFR 25.11 shall be included in the...

  19. 40 CFR 256.61 - Requirements for public participation in the annual State work program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES GUIDELINES FOR DEVELOPMENT AND IMPLEMENTATION OF STATE SOLID WASTE MANAGEMENT PLANS Public Participation § 256.61 Requirements for public participation in the annual State work program. (a) A public participation work plan in accord with 40 CFR 25.11 shall be included in the...

  20. 40 CFR 256.61 - Requirements for public participation in the annual State work program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES GUIDELINES FOR DEVELOPMENT AND IMPLEMENTATION OF STATE SOLID WASTE MANAGEMENT PLANS Public Participation § 256.61 Requirements for public participation in the annual State work program. (a) A public participation work plan in accord with 40 CFR 25.11 shall be included in the...

  1. 40 CFR 256.61 - Requirements for public participation in the annual State work program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES GUIDELINES FOR DEVELOPMENT AND IMPLEMENTATION OF STATE SOLID WASTE MANAGEMENT PLANS Public Participation § 256.61 Requirements for public participation in the annual State work program. (a) A public participation work plan in accord with 40 CFR 25.11 shall be included in the...

  2. Measuring Changes in Salaries and Wages in Public Schools. [Annual Edition].

    ERIC Educational Resources Information Center

    Educational Research Service, Arlington, VA.

    The Educational Research Service reports annually a statistical measure of salaries of public school system employees called the Composite Indicator of Changes (CIC) in Average Salaries and Wages Paid by Public School Systems. Each edition can be used to analyze trends in average salaries and wages paid to public school employees over the past 10…

  3. 78 FR 13912 - Sunshine Act; Cancellation Notice of Annual Public Hearing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ... From the Federal Register Online via the Government Publishing Office OVERSEAS PRIVATE INVESTMENT CORPORATION Sunshine Act; Cancellation Notice of Annual Public Hearing TIME AND DATE: 3:00 p.m., Wednesday...., Washington, DC. STATUS: Hearing OPEN to the Public at 3:00 p.m. PURPOSE: Public Hearing in conjunction...

  4. 76 FR 9044 - Notice of Extension of Information Collection for Public Comment; Consolidated Plan and Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-16

    ... URBAN DEVELOPMENT Notice of Extension of Information Collection for Public Comment; Consolidated Plan and Annual Performance Report AGENCY: Office of the Assistant Secretary for Community Planning and... proposed extension of information collection requirements for Consolidated Planning for Community...

  5. 77 FR 4824 - Notice of Revised Information Collection for Public Comment; Consolidated Plan & Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-31

    ... URBAN DEVELPOMENT Notice of Revised Information Collection for Public Comment; Consolidated Plan & Annual Performance Report AGENCY: Office of the Assistant Secretary for Community Planning and... information collection requirements for Consolidated Planning for Community Planning and Development...

  6. 76 FR 69769 - Annual Public Meeting of the Interagency Steering Committee on Multimedia Environmental Modeling

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-09

    ... COMMISSION Annual Public Meeting of the Interagency Steering Committee on Multimedia Environmental Modeling... the Federal Interagency Steering Committee on Multimedia Environmental Modeling (ISCMEM) will convene to discuss the latest developments in environmental modeling applications, tools and frameworks...

  7. Alabama Public Library Service, The State Library Agency. 1986 Annual Report.

    ERIC Educational Resources Information Center

    Alabama Public Library Service, Montgomery.

    Designed to provide an overview of the range and quality of services provided by the Alabama Public Library Service (APLS), this annual report focuses on the 1986 activities of APLS; the state's public library directory and statistical report will be published separately. A discussion of the activities of the Library Development Division includes…

  8. Statewide Report Card: An Annual Report to the Legislature on Oregon Public Schools, 2013-2014

    ERIC Educational Resources Information Center

    Oregon Department of Education, 2014

    2014-01-01

    The Oregon Statewide Report Card is an annual publication required by law (ORS 329.115), which reports on the state of public schools and their progress towards the goals of the Oregon Educational Act for the 21st Century. The purpose of the Oregon Report Card is to monitor trends among school districts and Oregon's progress toward achieving the…

  9. Statewide Report Card: An Annual Report to the Legislature on Oregon Public Schools, 2011-2012

    ERIC Educational Resources Information Center

    Oregon Department of Education, 2012

    2012-01-01

    The Oregon Statewide Report Card is an annual publication required by law (ORS 329.115), which reports on the state of public schools and their progress towards the goals of the Oregon Educational Act for the 21st Century. The purpose of the Oregon Report Card is to monitor trends among school districts and Oregon's progress toward achieving the…

  10. Student Charges at Public Institutions: Annual Survey 1988-89.

    ERIC Educational Resources Information Center

    Ludwig, Meredith; And Others

    The 1988 report on student charges at public colleges and universities compiled by the American Association of State Colleges and Universities (AASCU) and the National Association of State Universities and Land Grant Colleges (NASULGC) describes the costs of attending about 90% of the nation's publicly assisted, four-year institutions of higher…

  11. Student Charges at Public Institutions. Annual Survey 1989-90.

    ERIC Educational Resources Information Center

    Nathanson, Jeanne H.; Ludwig, Meredith J.

    The 1990 report on student charges at public colleges and universities describes the costs of attending about 90 percent of the national publicly assisted, four-year institutions. The report uses data compiled by the American Association of State Colleges and Universities (AASCU) and the National Association of State Universities and Land-Grand…

  12. The Corporation for Public Broadcasting. 1977 Annual Report.

    ERIC Educational Resources Information Center

    Corporation for Public Broadcasting, Washington, DC.

    Activities of the Corporation for Public Broadcasting (CPB) in its fiscal year ending September 30, 1977 are described in terms of television, radio, and other educational activities, as well as technology, legislation, long-term funding, human resources development, and general financing of public broadcasting. Other information includes CPB…

  13. Annual State of Public Education Report, 2012-2013

    ERIC Educational Resources Information Center

    Guam Department of Education, 2013

    2013-01-01

    This report addresses the reporting requirements of Public Law 26-26 and the provisions of "No Child Left Behind" ("NCLB") as described in the Guam Department of Education Board adopted "District Action Plan" ("DAP"). Public Law 26-26, Section 3106 states that "No later than thirty (30) days following…

  14. Taking Education's Pulse: The 13th Annual Gallup Poll of the Public's Attitudes Toward the Public Schools.

    ERIC Educational Resources Information Center

    Gallup, George H.

    This survey, which measures the attitudes of Americans toward their public schools, is the 13th annual survey in this series. New as well as trend questions are included in this and every survey. Personal, in-home interviews of 1519 adults were conducted in all areas of the nation and in all types of communities. The survey questions addressed:…

  15. Changing Expectations of College: The 47th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools

    ERIC Educational Resources Information Center

    Phi Delta Kappan, 2015

    2015-01-01

    This installment reporting on the 47th Annual PDK/Gallup Poll of American's Attitudes Toward the Public Schools focuses on American's views of the value of a high school and a college education and the affordability of college. It is the first year the poll included enough respondents to be able to break out sentiments of specific demographic…

  16. The ICR142 NGS validation series: a resource for orthogonal assessment of NGS analysis

    PubMed Central

    Ruark, Elise; Renwick, Anthony; Clarke, Matthew; Snape, Katie; Ramsay, Emma; Elliott, Anna; Hanks, Sandra; Strydom, Ann; Seal, Sheila; Rahman, Nazneen

    2016-01-01

    To provide a useful community resource for orthogonal assessment of NGS analysis software, we present the ICR142 NGS validation series. The dataset includes high-quality exome sequence data from 142 samples together with Sanger sequence data at 730 sites; 409 sites with variants and 321 sites at which variants were called by an NGS analysis tool, but no variant is present in the corresponding Sanger sequence. The dataset includes 286 indel variants and 275 negative indel sites, and thus the ICR142 validation dataset is of particular utility in evaluating indel calling performance. The FASTQ files and Sanger sequence results can be accessed in the European Genome-phenome Archive under the accession number EGAS00001001332. PMID:27158454

  17. 18 CFR 141.2 - FERC Form No. 1-F, Annual report for Nonmajor public utilities and licensees.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual report for Nonmajor public utilities and licensees. 141.2 Section 141.2 Conservation of Power and... ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978 STATEMENTS AND REPORTS (SCHEDULES) § 141.2 FERC Form No. 1-F, Annual report for Nonmajor public utilities and licensees. (a) Prescription. The form...

  18. 75 FR 76018 - Third Annual Sentinel Initiative Public Workshop

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-07

    .... Hosted by the Engelberg Center for Health Care Reform at The Brookings Institution, this 1-day public... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND.... Department of Health and Human Services efforts that use distributed systems of automated health care...

  19. Patenting genetic diagnostic methods: NGS, GWAS, SNPs and patents.

    PubMed

    Lawson, Charles

    2015-06-01

    This article reviews the problems posed by patent claims to genetic diagnostic methods associated with genome-wide association studies (GWAS) adopting methodologies using next-generation sequencing (NGS) and single nucleotide polymorphisms (SNP). These problems are essentially about experimental reproducibility and the credibility and veracity of reported developments. An analysis of the relevant law demonstrates that the current Australian and United States laws about suitable patentable subject matter differ, and that the current reproducibility (sufficiency, enablement and inutility) standards are unlikely to address these problems. The article concludes that following the United States approach excluding these genetic diagnostic method claims from patenting is one solution. Failing this, improving analysis and quality controls that are now being adopted in the basic research will reduce the nature of the problems, although this will remain problematic for patent examiners and the broader public. PMID:26349382

  20. Powerplant and Industrial Fuel Use Act. Annual report. [Annual report required by Section 108, Public Law 95-620

    SciTech Connect

    Not Available

    1983-03-01

    This annual report has been prepared for Congress by the Department of Energy in conjunction with the Environmental Protection Agency, as required by section 806 of the Powerplant and Industrial Fuel Use Act of 1978 (FUA) as amended, Public Law 95-620, enacted November 9, 1978. This annual report describes actions taken, under the provisions of the legislation, to encourage the use of indigenous energy resources in electric powerplants and major fuel-burning installations in the electric utility, industrial, and Federal governmental sectors. This report addresses implementation activities during calendar year 1982 under FUA and section 2 of the Energy Supply and Environmental Coordination Act of 1974, Public Law 93-319, and assesses the effectiveness of both laws in achieving their purposes. The report also includes summary information on natural gas consumption by electric utilities in 1977 and 1982, as required by section 711(c) of FUA, as amended by section 1021 of the Omnibus Budget Reconciliation Act of 1981, Public Law 97-35. Copies of this report were submitted to the Committee on Energy and Natural Resources of the US Senate and to the Committee on Energy and Commerce of the US House of Representatives, as required by section 807 of FUA. A companion report, as required by section 403(c) of FUA, will be submitted to Congress covering implementation of FUA at fuel-burning facilities owned or operated by the Federal Government.

  1. Publication outcome for research presented at the Vascular Society of Great Britain and Ireland annual meetings.

    PubMed

    Bhasin, N; Scott, D J A

    2007-04-01

    BACKGROUND The Vascular Society of Great Britain and Ireland (VSGBI) annual meeting is a major international vascular surgery conference. Studies suggest that the percentage of presentations that result in full-text publications are a measure of the quality of the meeting. We investigated the publication outcome of abstracts presented to the VSGBI in 2001 and 2002. MATERIALS AND METHODS We retrospectively identified abstracts from the conference programmes and conducted a detailed electronic Medline and PubMed search to determine publication. We collected data regarding the study design, subject matter,publishing journal, time to publication, institution of origin, impact factors and RAE levels. RESULTS There were 63 publications from 106 abstracts (59.4%), with a median impact factor of 3.507. Prospective observational studies accounted for 20.6% of publications, with abdominal aortic aneurysms being the commonest subject matter(34.9%). The median time to publication was 12 months, with the European Journal of Vascular and Endovascular Surgery publishing 33.3% of the articles. Leicester achieved the highest number of publications and the majority of work came from centres with Research Assessment Exercise (RAE) level scores of 4, university centres accounted for 74.6% of publications. CONCLUSIONS We conclude that when compared to equivalent meetings in other specialties and geographical regions, the annual meeting of the VSGBI is of the very highest quality. PMID:17431939

  2. The changing nature and scope of public health emergencies in response to annual flu.

    PubMed

    Hodge, James G

    2013-06-01

    The rapid spread of influenza during the 2012-13 season brought a series of public health challenges and corresponding response efforts. For decades, responses to annual flu have been undertaken routinely without extensive legal intervention. With the recent declaration of states of public health emergencies in Boston (January 9, 2013) and New York State (January 12, 2013), however, the legal baseline is changing. Propelled by a slate of state and local emergency declarations during the 2009-10 H1N1 pandemic, public officials are beginning to show cause for the issuance of formal emergency declarations in support of flu response efforts. The legal effects of these types of declarations are profound. Public and private actors are given significant, expedited public health powers. Scarce resources like vaccines can be more efficiently allocated. Laws relating to licensure, scope of practice, and liability can be effectively waived. Though originally conceptualized and once reserved for catastrophic, long-term health-related or bioterrorism events, public health emergency declarations are evolving to address temporary impacts on health care and public health services arising annually from flu outbreaks. This commentary explores the changing nature of public health emergencies and their current and potential impact on the provision of healthcare services in response to national or regional threats to the public's health. PMID:23641729

  3. State Governance Action Annual 2004. Balancing Act: Public Higher Education in Transition

    ERIC Educational Resources Information Center

    Johnson, Neal C.; Longanecker, David A.

    2004-01-01

    In this annual report, AGB's Center for Public Trusteeship and Governance provides an up-to-date and insightful description of current state-level policy discussions and actions, many of which could significantly alter the nature of higher education governance in America. Some to the ideas presented in this report may not, on the surface, appear…

  4. Measuring Changes in Salaries and Wages in Public Schools: 2000 Edition. 27th Annual Edition.

    ERIC Educational Resources Information Center

    Williams, Alicia R.; Cooke, Willa D.; Davis, Andrea M.; Miller, Oronde A.; Lewis, JoAnn

    This annual survey reports comparable salary data for 22 professional positions. The purpose of this edition is to aid in the analysis of trends in average salaries and wages paid public-school employees in the six component groups of school personnel over the previous 10 years. This report shows how the Composite Indicator of Changes (CIC) in…

  5. Annual Progress Report, 1976. Southern Rural Development Center, Mississippi State University. SRDC Series Publication No. 15.

    ERIC Educational Resources Information Center

    Southern Rural Development Center, State College, MS.

    Covering the 1976 activities of the Southern Rural Development Center (SRDC), this third annual report describes SRDC's: history; organization; regional workshops; functional networks; network bibliographies and other publications; Title V reports; grant received for training in rural development; orientation visits; consultants; information…

  6. 78 FR 17937 - Notice of Proposed Information Collection for Public Comment; FHA Lender Approval, Annual Renewal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection for Public Comment; FHA Lender Approval, Annual Renewal, Periodic Updates and Noncompliance Reporting by FHA Approved Lenders AGENCY: Office of...: Reports Liaison Officer, Department of Housing and Urban Development, 451 7th Street SW., Washington,...

  7. 75 FR 12251 - Notice of Proposed Information Collection for Public Comment; FHA Lender Approval, Annual Renewal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection for Public Comment; FHA Lender Approval, Annual Renewal, Periodic Updates and Noncompliance Reporting by FHA Approved Lenders AGENCY: Office of... Housing and Urban Development, 451 7th Street, SW., Washington, DC 20410; e-mail...

  8. 76 FR 34091 - Notice of Proposed Information Collection for Public Comment; FHA Lender Approval, Annual Renewal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-10

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection for Public Comment; FHA Lender Approval, Annual Renewal, Periodic Updates and Noncompliance Reporting by FHA Approved Lenders AGENCY: Office of...: Reports Liaison Officer, Department of Housing and Urban Development, 451 7th Street, SW., Washington,...

  9. 77 FR 63323 - Notice of Proposed Information Collection for Public Comment; FHA Lender Approval, Annual Renewal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-16

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection for Public Comment; FHA Lender Approval, Annual Renewal, Periodic Updates and Noncompliance Reporting by FHA Approved Lenders AGENCY: Office of...: Reports Liaison Officer, Department of Housing and Urban Development, 451 7th Street SW., Washington,...

  10. 78 FR 2684 - Notice of Proposed Information Collection for Public Comment: Annual Progress Report (APR) for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-14

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection for Public Comment: Annual Progress Report... Community Planning and Development, U.S. Department of Housing and Urban Development (HUD). ACTION: Notice... Urban Development, 451 7th Street SW., Room 4160, Washington, DC 20410-5000; telephone (202)...

  11. 76 FR 30921 - International Whaling Commission; 63rd Annual Meeting; Announcement of Public Meetings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... National Oceanic and Atmospheric Administration RIN 0648-XA459 International Whaling Commission; 63rd... annual International Whaling Commission (IWC) meeting. DATES: The public meetings will be held June 14... International Convention for the Regulation of Whaling, 1946. The U.S. Commissioner has responsibility for...

  12. 78 FR 59662 - Annual Public Meeting of the Interagency Steering Committee on Multimedia Environmental Modeling

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ...The annual public meeting of the Federal Interagency Steering Committee on Multimedia Environmental Modeling (ISCMEM) will convene to discuss the latest developments in environmental modeling applications, tools and frameworks as well as new operational initiatives for FY 2014 among the participating agencies. The meeting will be hosted by the U.S. Nuclear Regulatory Commission (U.S. NRC), one......

  13. Publication rates of abstracts presented at the annual meeting of the Society of Military Orthopaedic Surgeons.

    PubMed

    Fuller, Brian C; Dowd, Thomas C; Masini, Brendan D; Gerlinger, Tad L

    2012-01-01

    Previous studies have reported publication rates of abstracts presented at orthopaedic meetings from 22 to 68 percent. The objective of this study was to determine the publication rate of papers presented at the Society of Military Orthopaedic Surgeons (SOMOS) meetings from 1999 to 2003. A database was created including all abstracts presented at SOMOS meetings from 1999 to 2003 as listed in official program books. To assess whether each abstract resulted in publication in a peer-reviewed journal, a computerized PubMed search of the presenting author and appropriate keywords from the title was conducted. Overall, 191 of the 435 abstracts presented at SOMOS from 1999 to 2003 were published in a peer-reviewed journal, giving a publication rate of 44%. The publication rate of abstract presentations at annual SOMOS meetings compares favorably with the rates for other orthopaedic meetings. However, less than 50% result in peer-reviewed publication. PMID:22995357

  14. The 16th Annual Gallup Poll of the Public's Attitudes toward the Public Schools.

    ERIC Educational Resources Information Center

    Gallup, George H.

    1984-01-01

    Reports results from the 1984 survey, which found Americans respond more favorably to public schools than at any time in the past decade. Survey results show that they also feel education contributes more to national strength than industrial or military power. (MD)

  15. Fourth Annual Conference on Public and Non-Public Schools: Value Education. December 1, 1977. Proceedings.

    ERIC Educational Resources Information Center

    Lachman, Seymour P., Ed.

    Convened to offer a forum for discussion of values education to over 100 representatives of public and private schools, the conference explored moral dilemmas, educational trends, cognitive moral development, and educational objectives. The keynote address by values education exponent Dr. Edwin Fenton (Director, Carnegie-Mellon Education Center,…

  16. Orthogonal NGS for High Throughput Clinical Diagnostics

    PubMed Central

    Chennagiri, Niru; White, Eric J.; Frieden, Alexander; Lopez, Edgardo; Lieber, Daniel S.; Nikiforov, Anastasia; Ross, Tristen; Batorsky, Rebecca; Hansen, Sherry; Lip, Va; Luquette, Lovelace J.; Mauceli, Evan; Margulies, David; Milos, Patrice M.; Napolitano, Nichole; Nizzari, Marcia M.; Yu, Timothy; Thompson, John F.

    2016-01-01

    Next generation sequencing is a transformative technology for discovering and diagnosing genetic disorders. However, high-throughput sequencing remains error-prone, necessitating variant confirmation in order to meet the exacting demands of clinical diagnostic sequencing. To address this, we devised an orthogonal, dual platform approach employing complementary target capture and sequencing chemistries to improve speed and accuracy of variant calls at a genomic scale. We combined DNA selection by bait-based hybridization followed by Illumina NextSeq reversible terminator sequencing with DNA selection by amplification followed by Ion Proton semiconductor sequencing. This approach yields genomic scale orthogonal confirmation of ~95% of exome variants. Overall variant sensitivity improves as each method covers thousands of coding exons missed by the other. We conclude that orthogonal NGS offers improvements in variant calling sensitivity when two platforms are used, better specificity for variants identified on both platforms, and greatly reduces the time and expense of Sanger follow-up, thus enabling physicians to act on genomic results more quickly. PMID:27090146

  17. NGS-Trex: Next Generation Sequencing Transcriptome profile explorer

    PubMed Central

    2013-01-01

    Background Next-Generation Sequencing (NGS) technology has exceptionally increased the ability to sequence DNA in a massively parallel and cost-effective manner. Nevertheless, NGS data analysis requires bioinformatics skills and computational resources well beyond the possibilities of many "wet biology" laboratories. Moreover, most of projects only require few sequencing cycles and standard tools or workflows to carry out suitable analyses for the identification and annotation of genes, transcripts and splice variants found in the biological samples under investigation. These projects can take benefits from the availability of easy to use systems to automatically analyse sequences and to mine data without the preventive need of strong bioinformatics background and hardware infrastructure. Results To address this issue we developed an automatic system targeted to the analysis of NGS data obtained from large-scale transcriptome studies. This system, we named NGS-Trex (NGS Transcriptome profile explorer) is available through a simple web interface http://www.ngs-trex.org and allows the user to upload raw sequences and easily obtain an accurate characterization of the transcriptome profile after the setting of few parameters required to tune the analysis procedure. The system is also able to assess differential expression at both gene and transcript level (i.e. splicing isoforms) by comparing the expression profile of different samples. By using simple query forms the user can obtain list of genes, transcripts, splice sites ranked and filtered according to several criteria. Data can be viewed as tables, text files or through a simple genome browser which helps the visual inspection of the data. Conclusions NGS-Trex is a simple tool for RNA-Seq data analysis mainly targeted to "wet biology" researchers with limited bioinformatics skills. It offers simple data mining tools to explore transcriptome profiles of samples investigated taking advantage of NGS technologies

  18. CRCDA—Comprehensive resources for cancer NGS data analysis

    PubMed Central

    Thangam, Manonanthini; Gopal, Ramesh Kumar

    2015-01-01

    Next generation sequencing (NGS) innovations put a compelling landmark in life science and changed the direction of research in clinical oncology with its productivity to diagnose and treat cancer. The aim of our portal comprehensive resources for cancer NGS data analysis (CRCDA) is to provide a collection of different NGS tools and pipelines under diverse classes with cancer pathways and databases and furthermore, literature information from PubMed. The literature data was constrained to 18 most common cancer types such as breast cancer, colon cancer and other cancers that exhibit in worldwide population. NGS-cancer tools for the convenience have been categorized into cancer genomics, cancer transcriptomics, cancer epigenomics, quality control and visualization. Pipelines for variant detection, quality control and data analysis were listed to provide out-of-the box solution for NGS data analysis, which may help researchers to overcome challenges in selecting and configuring individual tools for analysing exome, whole genome and transcriptome data. An extensive search page was developed that can be queried by using (i) type of data [literature, gene data and sequence read archive (SRA) data] and (ii) type of cancer (selected based on global incidence and accessibility of data). For each category of analysis, variety of tools are available and the biggest challenge is in searching and using the right tool for the right application. The objective of the work is collecting tools in each category available at various places and arranging the tools and other data in a simple and user-friendly manner for biologists and oncologists to find information easier. To the best of our knowledge, we have collected and presented a comprehensive package of most of the resources available in cancer for NGS data analysis. Given these factors, we believe that this website will be an useful resource to the NGS research community working on cancer. Database URL: http://bioinfo.au-kbc.org.in/ngs

  19. Next generation sequencing (NGS)technologies and applications

    SciTech Connect

    Vuyisich, Momchilo

    2012-09-11

    NGS technology overview: (1) NGS library preparation - Nucleic acids extraction, Sample quality control, RNA conversion to cDNA, Addition of sequencing adapters, Quality control of library; (2) Sequencing - Clonal amplification of library fragments, (except PacBio), Sequencing by synthesis, Data output (reads and quality); and (3) Data analysis - Read mapping, Genome assembly, Gene expression, Operon structure, sRNA discovery, and Epigenetic analyses.

  20. CRCDA--Comprehensive resources for cancer NGS data analysis.

    PubMed

    Thangam, Manonanthini; Gopal, Ramesh Kumar

    2015-01-01

    Next generation sequencing (NGS) innovations put a compelling landmark in life science and changed the direction of research in clinical oncology with its productivity to diagnose and treat cancer. The aim of our portal comprehensive resources for cancer NGS data analysis (CRCDA) is to provide a collection of different NGS tools and pipelines under diverse classes with cancer pathways and databases and furthermore, literature information from PubMed. The literature data was constrained to 18 most common cancer types such as breast cancer, colon cancer and other cancers that exhibit in worldwide population. NGS-cancer tools for the convenience have been categorized into cancer genomics, cancer transcriptomics, cancer epigenomics, quality control and visualization. Pipelines for variant detection, quality control and data analysis were listed to provide out-of-the box solution for NGS data analysis, which may help researchers to overcome challenges in selecting and configuring individual tools for analysing exome, whole genome and transcriptome data. An extensive search page was developed that can be queried by using (i) type of data [literature, gene data and sequence read archive (SRA) data] and (ii) type of cancer (selected based on global incidence and accessibility of data). For each category of analysis, variety of tools are available and the biggest challenge is in searching and using the right tool for the right application. The objective of the work is collecting tools in each category available at various places and arranging the tools and other data in a simple and user-friendly manner for biologists and oncologists to find information easier. To the best of our knowledge, we have collected and presented a comprehensive package of most of the resources available in cancer for NGS data analysis. Given these factors, we believe that this website will be an useful resource to the NGS research community working on cancer. Database URL: http://bioinfo.au-kbc.org.in/ngs

  1. The Use of GOCE/GRACE Information in the Latest NGS xGeoid15 Model for the USA

    NASA Astrophysics Data System (ADS)

    Holmes, S. A.; Li, X.; Youngman, M.

    2015-12-01

    The U.S. National Geodetic Survey [NGS], through its Gravity for the Redefinition of the American Vertical Datum [GRAV-D] program, is flying airborne gravity surveys over the USA and its territories. By 2022, NGS intends that all orthometric heights in the USA will be determined in the field using a reliable national gravimetric geoid model to transform from geodetic heights obtained from GPS. Towards this end, all available airborne data has been incorporated into a new NGS experimental geoid model - xGEOID15. The xGEOID15 model is the second in a series of annual experimental geoid models that incorporates NGS GRAV-D airborne data. This series provides a useful benchmark for assessing and improving current techniques, to ultimately compute a geoid model that can support a national physical height system by 2022. Here, we focus on the combination of the latest GOCE/GRACE models with the terrestrial gravimetry (land/airborne) that was applied for xGeoid15. Comparisons against existing combination gravitational solutions, such as EGM2008 and EIGEN6C4, as well as recent geoid models, such as xGeoid14 and CGG2013, are interesting for what they reveal about the respective use of the GOCE/GRACE satgrav information.

  2. 75 FR 65340 - Secretarial Review and Publication of the Annual Report to Congress Submitted by the Contracted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... mandate for review and publication of the annual report on September 10, 2009 (74 FR 46594). In March 2010... different aspects of the healthcare system and are widely viewed as the ``gold standard'' for...

  3. Development of a high-resolution NGS-based HLA-typing and analysis pipeline

    PubMed Central

    Wittig, Michael; Anmarkrud, Jarl A.; Kässens, Jan C.; Koch, Simon; Forster, Michael; Ellinghaus, Eva; Hov, Johannes R.; Sauer, Sascha; Schimmler, Manfred; Ziemann, Malte; Görg, Siegfried; Jacob, Frank; Karlsen, Tom H.; Franke, Andre

    2015-01-01

    The human leukocyte antigen (HLA) complex contains the most polymorphic genes in the human genome. The classical HLA class I and II genes define the specificity of adaptive immune responses. Genetic variation at the HLA genes is associated with susceptibility to autoimmune and infectious diseases and plays a major role in transplantation medicine and immunology. Currently, the HLA genes are characterized using Sanger- or next-generation sequencing (NGS) of a limited amplicon repertoire or labeled oligonucleotides for allele-specific sequences. High-quality NGS-based methods are in proprietary use and not publicly available. Here, we introduce the first highly automated open-kit/open-source HLA-typing method for NGS. The method employs in-solution targeted capturing of the classical class I (HLA-A, HLA-B, HLA-C) and class II HLA genes (HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1, HLA-DPB1). The calling algorithm allows for highly confident allele-calling to three-field resolution (cDNA nucleotide variants). The method was validated on 357 commercially available DNA samples with known HLA alleles obtained by classical typing. Our results showed on average an accurate allele call rate of 0.99 in a fully automated manner, identifying also errors in the reference data. Finally, our method provides the flexibility to add further enrichment target regions. PMID:25753671

  4. Radiolytic Treatment of the Next-Generation Caustic-Side Solvent Extraction (NGS) Solvent and its Effect on the NGS Process

    SciTech Connect

    Roach, Benjamin D.; Williams, Neil J.; Duncan, Nathan C.; Delmau, Lætitia H.; Lee, Denise L.; Birdwell, Joseph F.; Moyer, Bruce A.

    2014-12-01

    We show in this work that the solvent used in the Next Generation Caustic-Side Solvent Extraction (NGS) process can withstand a radiation dose well in excess of the dose it would receive in multiple years of treating legacy salt waste at the US Department of Energy Savannah River Site. The solvent was subjected to a maximum of 50 kGy of gamma radiation while in dynamic contact with each of the aqueous phases of the current NGS process, namely SRS−15 (a highly caustic waste simulant), sodium hydroxide scrub solution (0.025 M), and boric acid strip solution (0.01 M). Bench-top testing of irradiated solvent confirmed that irradiation has inconsequential impact on the extraction, scrubbing, and stripping performance of the solvent up to 13 times the estimated 0.73 kGy/y annual absorbed dose. Lastly, stripping performance is the most sensitive step to radiation, deteriorating more due to buildup of p-sec-butylphenol (SBP) and possibly other proton-ionizable products than to degradation of the guanidine suppressor, as shown by chemical analyses.

  5. Radiolytic Treatment of the Next-Generation Caustic-Side Solvent Extraction (NGS) Solvent and its Effect on the NGS Process

    DOE PAGESBeta

    Roach, Benjamin D.; Williams, Neil J.; Duncan, Nathan C.; Delmau, Lætitia H.; Lee, Denise L.; Birdwell, Joseph F.; Moyer, Bruce A.

    2014-12-01

    We show in this work that the solvent used in the Next Generation Caustic-Side Solvent Extraction (NGS) process can withstand a radiation dose well in excess of the dose it would receive in multiple years of treating legacy salt waste at the US Department of Energy Savannah River Site. The solvent was subjected to a maximum of 50 kGy of gamma radiation while in dynamic contact with each of the aqueous phases of the current NGS process, namely SRS−15 (a highly caustic waste simulant), sodium hydroxide scrub solution (0.025 M), and boric acid strip solution (0.01 M). Bench-top testing ofmore » irradiated solvent confirmed that irradiation has inconsequential impact on the extraction, scrubbing, and stripping performance of the solvent up to 13 times the estimated 0.73 kGy/y annual absorbed dose. Lastly, stripping performance is the most sensitive step to radiation, deteriorating more due to buildup of p-sec-butylphenol (SBP) and possibly other proton-ionizable products than to degradation of the guanidine suppressor, as shown by chemical analyses.« less

  6. Americans Speak out: Are Educators and Policy Makers Listening?--The 40th Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    ERIC Educational Resources Information Center

    Bushaw, William J.; Gallup, Alec M.

    2008-01-01

    Parents, educators, political leaders--all Americans need the information contained in this article. But why this poll? First, it's not just a quick snapshot of public perception. Rather, it is a serious research tool, conducted annually, that allows Phi Delta Kappa (PDK) members and other educators and policy makers to track public opinion from…

  7. Americans Put Teacher Quality on Center Stage: The 46th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools. Part II

    ERIC Educational Resources Information Center

    Bushaw, William J.; Calderon, Valerie J.

    2014-01-01

    In this, the second installment of a two-part report of the 46th annual PDK/Gallup Poll of the Public's Attitudes Toward the Public Schools, the report unveils what Americans are thinking about public schools--in particular their views of teachers and the classrooms where they work. Some findings were surprising, others were not. For example,…

  8. Annual Statistical Report of the Public Schools of Arkansas, Public Charter Schools, and Education Service Cooperatives, 2013-2014 Actual and 2014-2015 Budgeted, (ASR)

    ERIC Educational Resources Information Center

    Arkansas Department of Education, 2015

    2015-01-01

    In compliance with the provisions of A.C.A.§§6-20-2201 et seq., the Annual Statistical Report of the Public Schools of Arkansas, Public Charter Schools, and Education Service Cooperatives, 2013-2014 Actual and 2014-2015 Budgeted, (ASR) is presented here. The Rankings of Selected Items of the Public Schools of Arkansas, 2013-2014 Actual, (Rankings)…

  9. 77 FR 56919 - Secretarial Review and Publication of the Annual Report to Congress Submitted by the Contracted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... publication of the annual report on September 10, 2009 (74 FR 46594). In March 2010, NQF submitted to Congress... FR 65340) to comply with the statutorily required Secretarial review and publication. In March 2011... the Federal Register on September 7, 2011 (76 FR 55474). Affordable Care Act was signed into law...

  10. 77 FR 58205 - Request for Public Comments on Annual Review of Country Eligibility for Benefits Under the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-19

    ... Subcommittee of the Trade Policy Staff Committee (the ``Subcommittee'') is requesting written public comments... make a submission at www.regulations.gov , please contact Don Eiss, Trade Policy Staff Committee, at... TRADE REPRESENTATIVE Request for Public Comments on Annual Review of Country Eligibility for...

  11. 75 FR 57492 - Notice of Submission of Proposed Information Collection to OMB Public Housing 5 Year Annual PHA Plan

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-21

    ... URBAN DEVELOPMENT Notice of Submission of Proposed Information Collection to OMB Public Housing 5 Year... public comments on the subject proposal. PHAs are required to submit annual and 5-Year Plans to HUD as required by section 5A of the United States Housing Act of 1937 (42 U.S.C. 1437c-1) The purpose of the...

  12. ngs.plot: Quick mining and visualization of next-generation sequencing data by integrating genomic databases

    PubMed Central

    2014-01-01

    Background Understanding the relationship between the millions of functional DNA elements and their protein regulators, and how they work in conjunction to manifest diverse phenotypes, is key to advancing our understanding of the mammalian genome. Next-generation sequencing technology is now used widely to probe these protein-DNA interactions and to profile gene expression at a genome-wide scale. As the cost of DNA sequencing continues to fall, the interpretation of the ever increasing amount of data generated represents a considerable challenge. Results We have developed ngs.plot – a standalone program to visualize enrichment patterns of DNA-interacting proteins at functionally important regions based on next-generation sequencing data. We demonstrate that ngs.plot is not only efficient but also scalable. We use a few examples to demonstrate that ngs.plot is easy to use and yet very powerful to generate figures that are publication ready. Conclusions We conclude that ngs.plot is a useful tool to help fill the gap between massive datasets and genomic information in this era of big sequencing data. PMID:24735413

  13. Public Utility Regulatory Policies Act of 1978. Annual report to Congress

    SciTech Connect

    None,

    1980-05-01

    The Public Utility Regulatory Policies Act of 1978 requires State regulatory authorities and nonregulated utilities to consider and make determinations regarding a set of Federal standards that show promise of furthering three statutory purposes: end-use conservation, utility efficiency, and equitable rates. PURPA sections 116 and 309 require the Secretary of Energy to report annually to Congress regarding the progress of these State regulatory authorities and nonregulated utilities in carrying out their PURPA obligations. DOE is also required to report on its own PURPA-related activities and to recommend any further Federal initiatives, including legislation, that may be necessary to carry out the purposes of the Act. In addition, section 206 of the Energy Conservation and Production Act of 1976 requires DOE to report annually regarding its activities under Title II of ECPA. This document fulfills these statutory reporting requirements for 1980, and assesses the progress made by State regulatory authorities and nonregulated utilities prior to June 30, 1979, in carrying out their PURPA duties and responsibilities. The report concludes that while there was more progress made on the regulatory standards than on the ratemaking standards, progress on all standards as of June 30, 1979, was limited. The report also describes DOE's efforts to assist State regulatory authorities and nonregulated utilities in carrying out their PURPA responsibilities.

  14. Try It Again, Uncle Sam: The 46th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools. [Part I

    ERIC Educational Resources Information Center

    Bushaw, William J.; Calderon, Valerie J.

    2014-01-01

    The 46th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools reveals that Americans say they know a lot more this year about the Common Core State Standards than they indicated a year ago, and they like it a lot less. Americans also say local school boards should have the greatest influence in deciding what is taught in…

  15. 26 CFR 301.6104(d)-1 - Public inspection and distribution of applications for tax exemption and annual information...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 18 2011-04-01 2011-04-01 false Public inspection and distribution of applications for tax exemption and annual information returns of tax-exempt organizations. 301.6104(d)-1 Section 301.6104(d)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) PROCEDURE AND ADMINISTRATION PROCEDURE...

  16. 78 FR 60367 - Request for Public Comments on Annual Review of Country Eligibility for Benefits Under the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-01

    ... Opportunity Act Implementation Subcommittee of the Trade Policy Staff Committee (``Subcommittee'') is... to make a submission at www.regulations.gov , please contact Yvonne Jamison, Trade Policy Staff... TRADE REPRESENTATIVE Request for Public Comments on Annual Review of Country Eligibility for...

  17. A Report to the People. 20 Years of Your National Commitment to Public Broadcasting, 1967-1987. 1986 Annual Report.

    ERIC Educational Resources Information Center

    Corporation for Public Broadcasting, Washington, DC.

    This annual report for the Corporation for Public Broadcasting (CPB) for fiscal year 1986 also summarizes the CPB's activities over the last 20 years. The front inside cover folds out to three pages and provides a chronology of the important events in CPB history from its inception in 1967 to 1987. A narrative report on the CPB's 20 years of…

  18. Multiple Object Adaptive Optics: Mixed NGS/LGS tomography

    NASA Astrophysics Data System (ADS)

    Morris, Tim; Gendron, Eric; Basden, Alastair; Martin, Olivier; Osborn, James; Henry, David; Hubert, Zoltan; Sivo, Gaetano; Gratadour, Damien; Chemla, Fanny; Sevin, Arnaud; Cohen, Matthieu; Younger, Eddy; Vidal, Fabrice; Wilson, Richard; Batterley, Tim; Bitenc, Urban; Reeves, Andrew; Bharmal, Nazim; Raynaud, Henri-François; Kulcsar, Caroline; Conan, Jean-Marc; Guzman, Dani; De Cos Juez, Javier; Huet, Jean-Michel; Perret, Denis; Dickson, Colin; Atkinson, David; Baillie, Tom; Longmore, Andy; Todd, Stephen; Talbot, Gordon; Morris, Simon; Myers, Richard; Rousset, Gérard

    2013-12-01

    Open-loop adaptive optics has been successfully demonstrated on-sky by several groups, including the fully tomographic MOAO demonstration made using CANARY. MOAO instrumentation such as RAVEN will deliver the first astronomical science and other planned instruments aim to extend both open-loop AO performance and the number of corrected fields. Many of these planned systems rely on the use of tomographic open-loop LGS wavefront sensing. Here we present results from the combined NGS/LGS tomographic CANARY system and then compare the NGS- and LGS-based tomographic system performance. We identify the major system performance drivers, and highlight some potential routes for further exploitation of open-loop tomographic AO.

  19. Make Teaching More Professional: The 47th Annual PDK/Gallup Poll of the Public's Attitudes toward the Public Schools

    ERIC Educational Resources Information Center

    Starr, Joshua P.

    2015-01-01

    Americans want higher professional requirements for teachers and believe teacher pay is too low, but they don't like tenure, according to the newest PDK/Gallup Poll of the Public's Attitudes To­ward the Public Schools. Public school parents trust and have confidence in the nation's teachers, and they said communicating with their child's teacher…

  20. COV2HTML: a visualization and analysis tool of bacterial next generation sequencing (NGS) data for postgenomics life scientists.

    PubMed

    Monot, Marc; Orgeur, Mickael; Camiade, Emilie; Brehier, Clément; Dupuy, Bruno

    2014-03-01

    COV2HTML is an interactive web interface, which is addressed to biologists, and allows performing both coverage visualization and analysis of NGS alignments performed on prokaryotic organisms (bacteria and phages). It combines two processes: a tool that converts the huge NGS mapping or coverage files into light specific coverage files containing information on genetic elements; and a visualization interface allowing a real-time analysis of data with optional integration of statistical results. To demonstrate the scope of COV2HTML, the program was tested with data from two published studies. The first data were from RNA-seq analysis of Campylobacter jejuni, based on comparison of two conditions with two replicates. We were able to recover 26 out of 27 genes highlighted in the publication using COV2HTML. The second data comprised of stranded TSS and RNA-seq data sets on the Archaea Sulfolobus solfataricus. COV2HTML was able to highlight most of the TSSs from the article and allows biologists to visualize both TSS and RNA-seq on the same screen. The strength of the COV2HTML interface is making possible NGS data analysis without software installation, login, or a long training period. A web version is accessible at https://mmonot.eu/COV2HTML/ . This website is free and open to users without any login requirement. PMID:24512253

  1. COV2HTML: A Visualization and Analysis Tool of Bacterial Next Generation Sequencing (NGS) Data for Postgenomics Life Scientists

    PubMed Central

    Orgeur, Mickael; Camiade, Emilie; Brehier, Clément; Dupuy, Bruno

    2014-01-01

    Abstract COV2HTML is an interactive web interface, which is addressed to biologists, and allows performing both coverage visualization and analysis of NGS alignments performed on prokaryotic organisms (bacteria and phages). It combines two processes: a tool that converts the huge NGS mapping or coverage files into light specific coverage files containing information on genetic elements; and a visualization interface allowing a real-time analysis of data with optional integration of statistical results. To demonstrate the scope of COV2HTML, the program was tested with data from two published studies. The first data were from RNA-seq analysis of Campylobacter jejuni, based on comparison of two conditions with two replicates. We were able to recover 26 out of 27 genes highlighted in the publication using COV2HTML. The second data comprised of stranded TSS and RNA-seq data sets on the Archaea Sulfolobus solfataricus. COV2HTML was able to highlight most of the TSSs from the article and allows biologists to visualize both TSS and RNA-seq on the same screen. The strength of the COV2HTML interface is making possible NGS data analysis without software installation, login, or a long training period. A web version is accessible at https://mmonot.eu/COV2HTML/. This website is free and open to users without any login requirement. PMID:24512253

  2. Annual Progress Report, 1975. Southern Rural Development Center, Mississippi State University. SRDC Series Publication No. 10.

    ERIC Educational Resources Information Center

    Southern Rural Development Center, State College, MS.

    Included in this second annual report on the Southern Rural Development Center's (SRDC) 1974-75 plan of work are data re: orientation visits; regional workshops; technical consultants; liaison with regional agencies and organizations; information dissemination; annual evaluation; functional networks in the areas of land use issues, citizen…

  3. ViennaNGS: A toolbox for building efficient next- generation sequencing analysis pipelines

    PubMed Central

    Wolfinger, Michael T.; Fallmann, Jörg; Eggenhofer, Florian; Amman, Fabian

    2015-01-01

    Recent achievements in next-generation sequencing (NGS) technologies lead to a high demand for reuseable software components to easily compile customized analysis workflows for big genomics data. We present ViennaNGS, an integrated collection of Perl modules focused on building efficient pipelines for NGS data processing. It comes with functionality for extracting and converting features from common NGS file formats, computation and evaluation of read mapping statistics, as well as normalization of RNA abundance. Moreover, ViennaNGS provides software components for identification and characterization of splice junctions from RNA-seq data, parsing and condensing sequence motif data, automated construction of Assembly and Track Hubs for the UCSC genome browser, as well as wrapper routines for a set of commonly used NGS command line tools. PMID:26236465

  4. SNPhood: investigate, quantify and visualise the epigenomic neighbourhood of SNPs using NGS data

    PubMed Central

    Arnold, Christian; Bhat, Pooja; Zaugg, Judith B.

    2016-01-01

    Motivation: The vast majority of the many thousands of disease-associated single nucleotide polymorphisms (SNPs) lie in the non-coding part of the genome. They are likely to affect regulatory elements, such as enhancers and promoters, rather than the function of a protein. To understand the molecular mechanisms underlying genetic diseases, it is therefore increasingly important to study the effect of a SNP on nearby molecular traits such as chromatin or transcription factor binding. Results: We developed SNPhood, a user-friendly Bioconductor R package to investigate, quantify and visualise the local epigenetic neighbourhood of a set of SNPs in terms of chromatin marks or TF binding sites using data from NGS experiments. Availability and implementation: SNPhood is publicly available and maintained as an R Bioconductor package at http://bioconductor.org/packages/SNPhood/. Contact: judith.zaugg@embl.de Supplementary information: Supplementary data are available at Bioinformatics online. PMID:27153574

  5. Maryland's Public Charter School Program: Providing High Quality Choices in Public Education. Charter School Annual Report, 2008

    ERIC Educational Resources Information Center

    Maryland State Department of Education, 2009

    2009-01-01

    The Maryland General Assembly enacted Maryland's charter school law in 2003. This current school year (2008-2009) marks the sixth year of Maryland's public charter school program. This paper presents the statistics representing the performance of Maryland's public charter schools for the school year 2008-2009.

  6. An NGS Workflow Blueprint for DNA Sequencing Data and Its Application in Individualized Molecular Oncology

    PubMed Central

    Li, Jian; Batcha, Aarif Mohamed Nazeer; Grüning, Björn; Mansmann, Ulrich R.

    2015-01-01

    Next-generation sequencing (NGS) technologies that have advanced rapidly in the past few years possess the potential to classify diseases, decipher the molecular code of related cell processes, identify targets for decision-making on targeted therapy or prevention strategies, and predict clinical treatment response. Thus, NGS is on its way to revolutionize oncology. With the help of NGS, we can draw a finer map for the genetic basis of diseases and can improve our understanding of diagnostic and prognostic applications and therapeutic methods. Despite these advantages and its potential, NGS is facing several critical challenges, including reduction of sequencing cost, enhancement of sequencing quality, improvement of technical simplicity and reliability, and development of semiautomated and integrated analysis workflow. In order to address these challenges, we conducted a literature research and summarized a four-stage NGS workflow for providing a systematic review on NGS-based analysis, explaining the strength and weakness of diverse NGS-based software tools, and elucidating its potential connection to individualized medicine. By presenting this four-stage NGS workflow, we try to provide a minimal structural layout required for NGS data storage and reproducibility. PMID:27081306

  7. 78 FR 59949 - Annual Factors for Determining Public Housing Agency Administrative Fees for the Section 8...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-30

    ...This notice announces the availability on HUD's Web site of the annual factors for determining the on-going administrative fee for housing agencies administering the rental voucher and moderate rehabilitation programs, including Single Room Occupancy during Calendar Year (CY)...

  8. Using Annual Data to Estimate the Public Health Impact of Extreme Temperatures.

    PubMed

    Goggins, William B; Yang, Chunyuh; Hokama, Tomiko; Law, Lewis S K; Chan, Emily Y Y

    2015-07-01

    Short-term associations between both hot and cold ambient temperatures and higher mortality have been found worldwide. Few studies have examined these associations on longer time scales. Age-standardized mortality rates (ASMRs) were calculated for 1976-2012 for Hong Kong SAR, People's Republic of China, defining "annual" time periods in 2 ways: from May through April of the following year and from November through October. Annual frequency and severity of extreme temperatures were summarized by using a degree-days approach with extreme heat expressed as annual degree-days >29.3°C and cold as annual degree-days <27.5°C. For example, a day with a mean temperature of 25.0°C contributes 2.5 cold degree-days to the annual total. Generalized additive models were used to estimate the association between annual hot and cold degree-days and the ASMR, with adjustment for long-term trends. Increases of 10 hot or 200 cold degree-days in an annual period, the approximate interquartile ranges for these variables, were significantly (all P's ≤ 0.011) associated with 1.9% or 3.1% increases, respectively, in the annual ASMR for the May-April analyses and with 2.2% or 2.8% increases, respectively, in the November-October analyses. Associations were stronger for noncancer and elderly mortality. Mortality increases associated with extreme temperature are not simply due to short-term forward displacement of deaths that would have occurred anyway within a few weeks. PMID:26009315

  9. Increasing Understanding of Public Problems and Policies: 1993. [Proceedings of the Annual Conference of the National Public Policy Education Committee (43rd, Clearwater Beach, Florida, September 12-15, 1993).

    ERIC Educational Resources Information Center

    Halbrook, Steve A., Ed.; Grace, Teddee E., Ed.

    The annual conference of the National Public Policy Education Committee (NPPEC) is held to improve the policy education efforts of extension workers responsible for public affairs programs. This publication contains 26 conference papers: "Rural America and the Information Revolution: An Exploration of Possibilities and Potentialities" (David…

  10. Annual report on the administration of the Radiation Control for Health and Safety Act of 1968, Public Law 90-602, (1988), April 1, 1989. Annual report

    SciTech Connect

    Not Available

    1989-04-01

    The Secretary of Health and Human Services is required by Subpart 3, Part F of Title III of the Public Health Service Act; 42 USC 263b et seq. (Public Law 90-602) to submit an annual report to the President for transmittal to the Congress on or before April 1 on the administration of the Radiation Control for Health and Safety Act. The detailed information required in the report is outlined in Section 360D of the Public Health Service Act. The Food and Drug Administration, through its Center for Devices and Radiological Health, is responsible for the day-to-day administration of the Radiation Control for Health and Safety Act of 1968. The report provides a summary of the operations of the Center in carrying out that responsibility for calendar year 1988. In reviewing the operations of the Center for Devices and Radiological Health as reported in the document, it should be kept in mind that the day-to-day administration of the Act is only part of the Center's function. Other responsibilities include the administration and enforcement of the 1976 Medical Device Amendments to the Federal Food, Drug, and Cosmetic Act (not covered in the report).

  11. NGS WFSs module for MAORY at E-ELT

    NASA Astrophysics Data System (ADS)

    Esposito, S.; Agapito, G.; Antichi, J.; Bonanno, A.; Carbonaro, L.; Giordano, C.; Spanò, P.

    We report on the natural guide star (NGS) wavefront sensors (WFS) module for MAORY, the multi-cojugate adaptive optics (MCAO) system for the ESO E-ELT. Three low-order, near-infrared (H-band), Shack-Hartmann sensors provide fast acquisition of the first 5 modes (tip, tilt, focus, astigmatism) on 3 natural guide stars over a 160 arcsec field of view. Three moderate-order (20x20), visible (600-800 nm), pyramid WFSs provide the slow Truth sensing to correct LGS wavefront estimates of low-order modes. These sensors are mounted onto three R-theta stages to patrol the field of view. The module is also equipped with a retractable, on-axis, high-order (80x80), visible, pyramid WFS for the single-conjugate AO (SCAO) mode of MAORY and MICADO. The visible WFSs share the same 80x80 pyramid WFS design. This choice enables also a MCAO NGS capability. Simulations show that Strehl ratios (SR) over 40% are reached with MCAO and three, 2x2 sub-apertures, NIR low-order WFSs working with H-mag=20 reference stars. In SCAO mode, 90% SR for a 8mag stars with a contrast down to 10-5, and 45% SR for a 16mag star, are achieved.

  12. 77 FR 61748 - Annual Public Meeting of the Interagency Steering Committee on Multimedia Environmental Modeling

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-11

    ... Public Meeting of the Interagency Steering Committee on Multimedia Environmental Modeling AGENCY: Office... public meeting of the Federal Interagency Steering Committee on Multimedia Environmental Modeling (ISCMEM) will convene to discuss the latest developments in environmental modeling applications, tools...

  13. Fifth Annual Conference on Public and Non-Public Schools: Education Faces the 80's and Beyond. Proceedings.

    ERIC Educational Resources Information Center

    Lachman, Seymour P., Ed.

    The purpose of this conference was to bring together leaders of public and private schools to discuss matters of mutual concern and interest. The morning session was concerned with the topic "The Child, the Teacher, the Machine" and focused on educational technology with emphasis on computer-aided instruction. The morning workshops dealt with "The…

  14. 77 FR 44656 - Notice of Annual Factors for Determining Public Housing Agency Administrative Fees for the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-30

    ... unit cost from a PHA's latest year-end settlement statement for 100 percent of expiring annual...-bedroom unit in a PHA's market area or the FY 1994 FMR for a two-bedroom unit, but not more than 103.5... implemented, which was calculated based on the amount each PHA was eligible to receive in CY 2003....

  15. Synthesis--An Annual Publication of The Higher Learning Commission, 2001.

    ERIC Educational Resources Information Center

    North Central Association of Colleges and Schools, Chicago, IL. Higher Learning Commission.

    This report, first of a projected annual series, focuses on the key commitments of the mission statement of the Higher Learning Commission of the North Central Association of Colleges and Schools and highlights new Commission initiatives. The issue opens with two essays related to the Commission's purposes: "Serving the Common Good in a Diverse…

  16. Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education, 2008. Annual Report

    ERIC Educational Resources Information Center

    Connecticut Department of Higher Education (NJ1), 2008

    2008-01-01

    Increasing the participation of minority groups at public colleges and universities is a longstanding goal of the Board of Governors for Higher Education, as first outlined in its 1983 "Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education." The minority groups defined by the plan are: Hispanic/Latino,…

  17. Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education, 2010. Annual Report

    ERIC Educational Resources Information Center

    Connecticut Department of Higher Education (NJ1), 2010

    2010-01-01

    Increasing the participation of minority groups at public colleges and universities is a longstanding goal of the Board of Governors for Higher Education, as first outlined in its 1983 "Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education". The minority groups defined by the plan are: Hispanic/Latino, African…

  18. Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education, 2011. Annual Report

    ERIC Educational Resources Information Center

    Connecticut Department of Higher Education (NJ1), 2011

    2011-01-01

    Increasing the participation of minority groups at public colleges and universities is a longstanding goal of the Board of Governors for Higher Education, as first outlined in its 1983 "Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education." The minority groups defined by the plan are: Hispanic/Latino,…

  19. Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education, 2009. Annual Report

    ERIC Educational Resources Information Center

    Connecticut Department of Higher Education (NJ1), 2009

    2009-01-01

    Increasing the participation of minority groups at public colleges and universities is a longstanding goal of the Board of Governors for Higher Education, as first outlined in its 1983 "Strategic Plan to Ensure Racial and Ethnic Diversity in Connecticut Public Higher Education". The minority groups defined by the plan are: Hispanic/Latino, African…

  20. Denver Public Schools, Division of Health Services. Forty-Sixth Annual Report, 1970-1971.

    ERIC Educational Resources Information Center

    Denver Public Schools, CO.

    This publication from the Division of Health Services in the Denver Public Schools details new projects initiated in 1970-71. Some of these projects include a) extension of dental care in kindergarten through seventh grade and among pupils in the Neighborhood Youth Corps, b) initiation of rubella immunization centers in the elementary schools, c)…

  1. A slow death or eternal life? Future directions for annual public health reports.

    PubMed

    Bruce, N; Price, C; Whincup, P

    1994-11-01

    Several years after their reintroduction, district public health reports continue to face questions about purpose, content and evaluation. As public health embraces the wider vision of the World Health Organisation's 'Health for All' strategy, and the consequent need for collaboration with other sectors and participation with communities, there is a need for the reports to respond accordingly. This theme is explored in relation to the needs of the report's diverse audience, the type of local research required to make the report relevant and effective, and approaches to evaluation. A growing local research function has resource and training implications for Departments of Public Health. The demands of producing a report every year suggest that biennial publication of the main report, with other targeted products over the intervening period, would be a more realistic and effective option. PMID:7997494

  2. Radiogenic age and isotopic studies, Report 3. Paper No. 89-02. Annual publication

    SciTech Connect

    Not Available

    1990-01-01

    Annual collection of reports presenting data from the Geochronology Section of the Continental Geoscience Division. Reports make full presentation of the data, relate these to field settings, and make comparatively short interpretations. The reports cover Rb-Sr and Sm-Nd procedures currently in use at the laboratory using high performance liquid chromatography methods; 40Ar/39Ar analyses, including that on the age of the New Quebec Crater; a summary of studies in N.B.; and studies in the northwest Canadian Shield, Baffin Island, Ontario, and Yukon Territory.

  3. Documenting stewardship responsibilities across the annual cycle for birds on U.S. public lands.

    PubMed

    La Sorte, Frank A; Fink, Daniel; Hochachka, Wesley M; Aycrigg, Jocelyn L; Rosenberg, Kenneth V; Rodewald, Amanda D; Bruns, Nicholas E; Farnsworth, Andrew; Sullivan, Brian L; Wood, Chris; Kelling, Steve

    2015-01-01

    In the face of global environmental change, the importance of protected areas in biological management and conservation is expected to grow. Birds have played an important role as biological indicators of the effectiveness of protected areas, but with little consideration given to where species occur outside the breeding season. We estimated weekly probability of occurrence for 308 bird species throughout the year within protected areas in the western contiguous USA using eBird occurrence data for the combined period 2004 to 2011. We classified species based on their annual patterns of occurrence on lands having intermediate conservation mandates (GAP status 2 and 3) administered by the Bureau of Land Management (BLM) and the United States Forest Service (USFS). We identified species having consistent annual association with one agency, and species whose associations across the annual cycle switched between agencies. BLM and USFS GAP status 2 and 3 lands contained low to moderate proportions of species occurrences, with proportions highest for species that occurred year-round or only during the summer. We identified two groups of species whose annual movements resulted in changes in stewardship responsibilities: (1) year-round species that occurred on USFS lands during the breeding season and BLM lands during the nonbreeding season; and (2) summer species that occurred on USFS lands during the breeding season and BLM lands during spring and autumn migration. Species that switched agencies had broad distributions, bred on high-elevation USFS lands, were not more likely to be identified as species of special management concern, and migrated short (year-round species) to long distances (summer species). Our findings suggest cooperative efforts that address the requirements of short-distance migratory species on GAP status 2 lands (n = 20 species) and GAP status 3 lands (n = 24) and long-distance migratory species on GAP status 2 lands (n = 9) would likely benefit

  4. VizieR Online Data Catalog: DUSTiNGS. I. The Good Source Catalog (Boyer+, 2015)

    NASA Astrophysics Data System (ADS)

    Boyer, M. L.; McQuinn, K. B. W.; Barmby, P.; Bonanos, A. Z.; Gehrz, R. D.; Gordon, K. D.; Groenewegen, M. A. T.; Lagadec, E.; Lennon, D.; Marengo, M.; Meixner, M.; Skillman, E.; Sloan, G. C.; Sonneborn, G.; van Loon, J. Th.; Zijlstra, A.

    2015-07-01

    The DUSTiNGS (DUST in Nearby Galaxies with Spitzer) survey includes uniform 3.6 and 4.5um Spitzer/IRAC imaging of 50 nearby galaxies (see table 1) between 2011 Jun 19 and 2012 Jun 23 (see table 2). The final Vega magnitudes of the high-quality point sources are reported in the DUSTiNGS "Good"-Source Catalog (GSC), which is described in Table 5 (see section 5). A full point source catalog is available at: http://irsa.ipac.caltech.edu/data/SPITZER/DUSTiNGS/ (3 data files).

  5. Student Charges at Public, Four-Year Institutions. Annual Survey, Fall 1992.

    ERIC Educational Resources Information Center

    Strand, Heather; And Others

    This report examines student charges at public, four-year institutions throughout the United States based on the responses from a survey of 513 responding institutions. Statistical data are presented in the appendix comprising nearly the entire report. Major findings include the following items: (1) the overall fall 1992 resident undergraduate…

  6. Fund Raising Outcomes and Effectiveness in Private and Public Institutions. ASHE Annual Meeting Paper.

    ERIC Educational Resources Information Center

    Duronio, Margaret A.; Loessin, Bruce A.

    An analysis of fund raising outcome for private and public institutions is presented for the 5-year period between 1983/84 and 1987/88. The results of research involving intensive case studies of fund raising practices and policies in 10 dissimilar institutions with successful fund raising programs are offered. Fund raising outcomes for the…

  7. Montgomery County Public Schools Annual Report to the Community, 2014 [Summary

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2015

    2015-01-01

    Montgomery County Public Schools (MCPS) is more than a school system, it is a community. It's a community of people working together to provide Montgomery County students with an education that prepares them for success today, tomorrow, and in the years to come. That is MCPS' core purpose: to prepare students to thrive in their future. The Annual…

  8. Annual Financial Reporting Requirements for Texas Public Community Colleges, Fiscal Year 1996.

    ERIC Educational Resources Information Center

    Texas State Higher Education Coordinating Board, Austin. Div. of Research, Planning and Finance.

    This manual provides a comprehensive set of definitions, rules, formats, and illustrations for use by public community and junior colleges in Texas to ensure consistent and uniform financial reporting. Chapter 1 provides an introduction to the manual, highlighting changes in requirements for Fiscal Year (FY) 1996. Chapter 2 presents general…

  9. SPIRES (Stanford Public Information Retrieval System) 1970-71 Annual Report.

    ERIC Educational Resources Information Center

    Parker, Edwin B.

    SPIRES (Stanford Public Information REtrieval System) is a computer information storage and retrieval system being developed at Stanford University with funding from the National Science Foundation. SPIRES has two major goals: to provide a user-oriented, interactive, on-line retrieval system for a variety of researchers at Stanford; and to support…

  10. Annual Research Review: Resilience and Child Well-Being--Public Policy Implications

    ERIC Educational Resources Information Center

    Ager, Alastair

    2013-01-01

    Background: There has been an 8-fold increase in use of the term resilience within scientific and scholar literature over the last twenty years. The arena of public policy has also seen increasing use made of the concept, both with respect to child well-being and development and wider issues. Method: A focal sample of literature comprising 108…

  11. Denver Public Schools, Department of Health Services. Forty-Eighth Annual Report, 1972-1973.

    ERIC Educational Resources Information Center

    Denver Public Schools, CO.

    This report details the programs and activities of the Department of Health Services of the Denver Public Schools, 1972-73. The first section, New Projects and Special Activities of the Department, presents health programs initiated in elementary and secondary schools and among adult employees. The development of a joint plan to give community…

  12. CANARY: The NGS/LGS MOAO demonstrator for EAGLE

    NASA Astrophysics Data System (ADS)

    Morris, Tim; Hubert, Zoltan; Myers, Richard; Gendron, Eric; Longmore, Andy; Rousset, Gerard; Talbot, Gordon; Fusco, Thierry; Dipper, Nigel; Vidal, Fabrice; Henry, David; Gratadour, Damien; Butterley, Tim; Chemla, Fanny; Guzman, Dani; Laporte, Phillipe; Younger, Eddy; Kellerer, Aglae; Harrison, Mark; Marteaud, Michel; Geng, Deli; Basden, Ali; Guesalaga, Andres; Dunlop, Colin; Todd, Steven; Robert, Clelia; Dee, Kevin; Dickson, Colin; Vedrenne, Nicolas; Greenaway, Alan; Stobie, Brian; Dalgarno, Heather; Skvarc, Jure

    EAGLE is a multi-object 3D spectroscopy instrument currently under design for the 42-metre European Extremely Large Telescope (E-ELT). EAGLE will use open-loop Multi-Object Adaptive Optics (MOAO) to provide partial AO correction across a wide (5-10 arcmin) field of view. The novelty of this scheme is such that on-sky demonstration is required prior to final construction of an E-ELT instrument. The CANARY project will implement a single channel of an MOAO system on the 4.2m William Herschel Telescope. The CANARY project is undergoing a phased development plan that starts with demonstration of low-order open-loop AO correction using first NGS then Rayleigh LGS tomography, moving to a demonstration of high-order open-loop AO correction using LGS tomography. This final stage will also include 2 DMs in a woofer-tweeter configuration similar to that of EAGLE when installed at the E-ELT. We describe the requirements for the various phases of MOAO demonstration, the corresponding CANARY configurations and capabilities and the current designs of the various subsystems.

  13. Electric and Magnetic Fields Research and Public Information Dissemination Program annual report for fiscal year 1996

    SciTech Connect

    1997-06-01

    The Electric and Magnetic Fields (EMF) Research and Public Information Dissemination (RAPID) Program was authorized by the Energy Policy Act of 1992 as a near-term effort to expand and accelerate the research needed to address the EMF issue. As required by this legislation, the EMF Interagency Committee, the National EMF Advisory Committee (NEMFAC), and the National Academy of Sciences (NAS) are providing valued input and advice for the direction of this program. With this input and advice, the Department of Energy (DOE) and the National Institute of Environmental Health Sciences (NIEHS) have developed and are implementing five-year program plans. Multi-year health effects research projects and related EMF measurement and exposure assessment projects are underway using funds appropriated in fiscal years 1994, 1995, and 1996 together with voluntary non-Federal contributions. The results of these research projects, along with the results of other EMF research, will be used as input to the hazard evaluation effort, which is the focus of the EMF RAPID Program. A coordinated interagency program is underway to communicate needed information on the EMF issue in a clear manner to the public and other decision makers.

  14. Publication rates of manuscript presentations at the American College of Foot and Ankle Surgeons Annual Scientific Conference between 1999 and 2008.

    PubMed

    Roukis, Thomas S

    2011-01-01

    Publication is the ultimate desired end point of scientific research. However, oral manuscript presentations of research studies are often referenced in textbooks, journal articles, and industry white papers, and, as a result, influence treatment care plans. No data exist for the actual publication rate of podiatric foot and ankle surgery oral manuscript presentations. Therefore, the objective of this study was to determine the actual publication rates of oral manuscript presentations at the American College of Foot and Ankle Surgeons (ACFAS) Annual Scientific Conference over 10 years. Print or electronic media for the ACFAS Annual Scientific Conference official program between 1999 and 2008 were obtained. Each year's official program was hand searched for any oral manuscript presentation, and, when identified, the title and authors were individually searched through electronic internet-based search engines to determine whether an oral manuscript presentation had been followed by publication of a full-text article. Additionally, pertinent journals were hand searched for potential articles. A total of 67.5% (139/206) oral manuscript presentations were ultimately published in 1 of 12 medical journals in a mean of 14.5 months. All journals except one (91.7%) represented peer-reviewed journals. The publication rate of oral manuscript presentations at the ACFAS Annual Scientific Conference is similar to or greater than orthopaedic subspecialties, including foot and ankle surgery, publication rates. Based on the above, attendees of the ACFAS Annual Scientific Conference should be aware that the majority of oral manuscript material presented at the ACFAS Annual Scientific Conference can be considered as accurate because they survive the rigors of the peer-review process more than two thirds of the time. PMID:21549618

  15. Fiscal Year 1979 Annual Program Plan Amendment for Part B of the Education of the Handicapped Act as Amended by Public Law 94-142.

    ERIC Educational Resources Information Center

    Oregon State Dept. of Education, Salem.

    The Oregon Annual Program Plan for fiscal year 1979 sets forth the state's policies to provide all handicapped childre (6-20 years old) with a free appropriate public education, as mandated by P.L. 94-142, the Education for All Handicapped Children Act. Addressed are the following topics: right to education, full educational opportunity timetable,…

  16. Public Utility Regulatory Policies Act of 1978. Annual report to Congress

    SciTech Connect

    None,

    1980-05-01

    Titles I and III of the Public Utility Regulatory Policies Act of 1978 (PURPA) establish retail regulatory policies for electric and natural gas utilities, respectively, aimed at achieving three purposes: conservation of energy supplied by electric and gas utilities; efficiency in the use of facilities and resources by these utilities; equitable rates to electricity and natural gas consumers. PURPA also continues the pilot utility implementation program, authorized under Title II of the Energy Conservation and Production ACT (ECPA), to encourage adoption of cost-based rates and efficient energy-management practices. The purpose of this report is twofold: (1) to summarize and analyze the progress that state regulatory authorities and certain nonregulated utilities have made in their consideration of the PURPA standards; and (2) to summarize the Department of Energy (DOE) activities relating to PURPA and ECPA. The report provides a broad overview and assessment of the status of electric and gas regulation nationwide, and thus helps provide the basis for congressional and DOE actions targeted on the utility industry to address pressing national energy problems.

  17. Wages and Salaries Paid Support Personnel in Public Schools, 1994-95. Part 3 of National Survey of Salaries and Wages in Public Schools. 22nd Annual Edition. ERS Report.

    ERIC Educational Resources Information Center

    Educational Research Service, Arlington, VA.

    This document is the third in a series that presents data from the National Survey of Salaries and Wages in Public Schools. The survey of a national stratified sample of 1,818 school systems elicited 1,019 responses, an overall response rate of 56 percent. The volume reports hourly wage rates and annual salaries actually being paid during the…

  18. Publications

    Cancer.gov

    Information about NCI publications including PDQ cancer information for patients and health professionals, patient-education publications, fact sheets, dictionaries, NCI blogs and newsletters and major reports.

  19. 78 FR 48421 - Publication of North American Datum of 1983 (2011) Epoch 2010.00, North American Datum of 1983...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ..., 54 FR 25318, June 14, 1989). DATES: Individuals or organizations wishing to submit comments on the...) Epoch 2010.00 AGENCY: National Geodetic Survey (NGS), National Ocean Service (NOS), National Oceanic and... Geodetic Survey (NGS) has finalized the publication of new realizations of three geodetic references...

  20. NGS-Trex: an automatic analysis workflow for RNA-Seq data.

    PubMed

    Boria, Ilenia; Boatti, Lara; Saggese, Igor; Mignone, Flavio

    2015-01-01

    RNA-Seq technology allows the rapid analysis of whole transcriptomes taking advantage of next-generation sequencing platforms. Moreover with the constant decrease of the cost of NGS analysis RNA-Seq is becoming very popular and widespread. Unfortunately data analysis is quite demanding in terms of bioinformatic skills and infrastructures required, thus limiting the potential users of this method. Here we describe the complete analysis of sample data from raw sequences to data mining of results by using NGS-Trex platform, a low user interaction, fully automatic analysis workflow. Used through a web interface, NGS-Trex processes data and profiles the transcriptome of the samples identifying expressed genes, transcripts, and new and known splice variants. It also detects differentially expressed genes and transcripts across different experiments. PMID:25577383

  1. RESULTS OF ANALYSIS OF NGS CONCENTRATE DRUM SAMPLES [Next Generation Solvent

    SciTech Connect

    Peters, T.; Williams, M.

    2013-09-13

    Savannah River National Laboratory (SRNL) prepared two drums (50 gallons each in �Drum#2� and �Drum#4�) of NGS-MCU (Next Generation Solvent-Modular CSSX Unit) concentrate for future use at MCU in downblending the BOBCalixC6 based solvent to produce NGS-MCU solvent. Samples of each drum were sent for analysis. The results of all the analyses indicate that the blend concentrate is of the correct composition and should produce a blended solvent at MCU of the desired formulation.

  2. 76 FR 55473 - Secretarial Review and Publication of the Annual Report to Congress Submitted by the Contracted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ... annual report on September 10, 2009 (74 FR 46594). In March 2010, NQF submitted to Congress and the..., 2010. The second annual report was published in the Federal Register on October 22, 2010 (75 FR 65340... obstructive pulmonary disease (9) Acute myocardial infarction (10) Colorectal cancer (11) Hip/pelvic...

  3. A Web-Hosted R Workflow to Simplify and Automate the Analysis of 16S NGS Data

    EPA Science Inventory

    Next-Generation Sequencing (NGS) produces large data sets that include tens-of-thousands of sequence reads per sample. For analysis of bacterial diversity, 16S NGS sequences are typically analyzed in a workflow that containing best-of-breed bioinformatics packages that may levera...

  4. Publications.

    ERIC Educational Resources Information Center

    Aviation/Space, 1980

    1980-01-01

    Presents a variety of publications available from government and nongovernment sources. The government publications are from the Federal Aviation Administration (FAA) and the National Aeronautics and Space Administration (NASA) and are designed for educators, students, and the public. (Author/SA)

  5. An Assessment of Airborn Gravimetry Collected under the NGS GRAV-D Project

    NASA Astrophysics Data System (ADS)

    Holmes, S. A.

    2009-12-01

    In the United States, the National Geodetic Survey [NGS] holds the official charter to maintain the vertical datum for the USA and its territories. This includes the responsibility to maintain a current geoid model for transforming between orthometric (H) and geodetic (h) heights. The latest (2009) NGS geoid model incorporates the latest GRACE-based satellite-only geopotential solutions, very-high-resolution digital elevation models [DEMs] derived from the Shuttle Radar Topography Mission [SRTM], and other additional data and processing improvements. This recent geoid model also benefited greatly from the prior release of the National Geospatial-Intelligence Agency’s [NGA] Earth Gravitational Model 2008 [EGM2008], which served as a computational reference field for the new geoid, but was also particularly useful for identifying and removing corrupted data from the NGS gravimetry database. Looking forwards, NGS intends to increase its efforts to refine and improve its future national geoid models. To this end, their ambitious "Gravity for the Re-definition of the American Datum” [GRAV-D] project aims to update the NGS gravimetry holdings by flying new airborn gravity surveys over a large fraction of the USA and it territories. Concurrent efforts will focus on developing new processing techniques for optimally incorporating improved gravimetry into the final geoid solution. To this end, the GRAV-D team has already flown several surveys in the Gulf of Mexico, Puerto Rico, US Virgin Islands, and Alaska. Testing and analysis aimed at calibrating and validating the preliminary survey data is already underway. The latest assessment of these recent efforts, including the extent to which this new data can be expected to contribute to an improved gravimetric geoid model, is presented here.

  6. Publication rates of abstracts presented at annual scientific meetings: how does the Royal Australian and New Zealand College of Radiologists compare?

    PubMed

    Bydder, Sean A; Joseph, David J; Spry, Nigel A

    2004-03-01

    The abstract to publication ratio (APR) is a measure of the quality of scientific meetings. The aim of the present study was to determine the publication rate of abstracts presented at annual Royal Australian and New Zealand College of Radiologists (RANZCR) conferences, and to identify the publishing journals. All free paper research abstracts (oral or poster) presented by RANZCR radiologists, radiation oncologists and trainees at the four consecutive meetings between 1996 and 1999 were identified retrospectively from conference programmes. The PubMed database (http://www.ncbi.nlm.nih.gov/PubMed/) was searched to determine whether or not the abstract had been published as a full paper. Of the 480 free paper research abstracts, 168 (35%) had been published as full articles. The overall abstract to publication ratio for radiology was 29% and for radiation oncology was 41%. Papers were published in a variety of journals but Australasian Radiology accounted for 27%. The mean time between presentation and publication was 16.5 months (median 17 months). These overall abstract to publication ratios are lower than those reported for overseas-based meetings in each respective area. Guidelines to scientific committees could increase the APR by more rigorous selection of abstracts. Future research should look at barriers to the publication of research findings, and identify ways to assist the publication process. PMID:15027917

  7. National Academy of Sciences, National Academy of Engineering, Institute of Medicine, National Research Council, Annual Report Fiscal Year 1975-76. Committee on Labor and Public Welfare, 94th Congress, 2nd Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Committee on Labor and Public Welfare.

    Presented is the annual report for fiscal year 1975-76 of the National Academy of Sciences (NAS), including the annual reports of the National Academy of Engineering and the Institute of Medicine. Contents include: (1) report of the President; (2) reports on completed studies including food and nutrition, public health, natural resources, space…

  8. Scheduled Salaries for Professional Personnel in Public Schools, 1999-2000. ERS Report: Part 1. National Survey of Salaries and Wages in Public Schools. 27th Annual Edition.

    ERIC Educational Resources Information Center

    Williams, Alicia R.; Cooke, Willa D.; Davis, Andrea M.; Miller, Oronde A.; Lewis, JoAnn

    This is part 1 of a 3-part report on a survey conducted in Fall 1999 of salaries and wages in public schools. The purpose of the survey is to provide school administrators and others concerned with the determination of salaries and wages for public-school personnel with data useful in comparing employee compensation in school systems of similar…

  9. Journal Publication of Material Presented at the 1967 Annual Meeting of the Geophysical Union During the Year Following the Meeting.

    ERIC Educational Resources Information Center

    Johns Hopkins Univ., Baltimore, MD. Center for Research in Scientific Communication.

    The April 1967 Annual Meeting of the American Geophysical Union was the subject of an investigation of scientific information exchange among geophysicists. The study focused on meeting presentation papers and drew a sample of 240 of the 800 presentation authors. The results of the meeting study demonstrated the currency of the work reported by…

  10. The Power of Possibility. Defining Moments: How IDRA and Its Partners Are Changing Public Education. IDRA Annual Report 2014

    ERIC Educational Resources Information Center

    Intercultural Development Research Association, 2014

    2014-01-01

    Intercultural Development Research Association's (IDRA) 2014 Annual Report highlights seven defining moments in the work of the previous year: (1) Securing Opportunity; (2) Defending Children's Educational and Civil Rights; (3) Building a National Grassroots Family Model; (4) Putting Quality Early Education at the Forefront; (5) Empowering…

  11. 78 FR 46695 - Secretarial Review and Publication of the Annual Report to Congress Submitted by the Contracted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-01

    ... 10, 2009 (74 FR 46594). In March 2010, NQF submitted to Congress and the Secretary the second annual... report was published in the Federal Register on October 22, 2010 (75 FR 65340) to comply with the... February 28, 2011, was published in the Federal Register on September 7, 2011 (76 FR 55474). In March...

  12. Environmental Sciences Division annual progress report for period ending September 30, 1982. Environmental Sciences Division Publication No. 2090. [Lead abstract

    SciTech Connect

    Not Available

    1983-04-01

    Separate abstracts were prepared for 12 of the 14 sections of the Environmental Sciences Division annual progress report. The other 2 sections deal with educational activities. The programs discussed deal with advanced fuel energy, toxic substances, environmental impacts of various energy technologies, biomass, low-level radioactive waste management, the global carbon cycle, and aquatic and terrestrial ecology. (KRM)

  13. Publication rates of poster presentations at the American College of Foot and Ankle Surgeons annual scientific conference between 1999 and 2008.

    PubMed

    Abicht, Bradley P; Donnenwerth, Michael P; Borkosky, Sara L; Plovanich, Elizabeth J; Roukis, Thomas S

    2012-01-01

    Publication is the desired end point of scientific research. Ultimately, it is desired that research presented in poster format at a scientific conference will be developed into a report and become published in a peer-reviewed scientific journal. Moreover, poster presentations of research studies are often referenced and, as a result, influence treatment care plans. No data exist for the actual publication rate of podiatric foot and ankle surgery poster presentations. Therefore, the objective of the present study was to determine the actual publication rates of poster presentations at the American College of Foot and Ankle Surgeons (ACFAS) annual scientific conference (ASC) during a 10-year period. Print or electronic media for the ACFAS ASC official program from 1999 to 2008 were obtained. Each year's official program was manually searched for any poster presentation and, when identified, the authors and title were individually searched using Internet-based search engines to determine whether a poster presentation had been followed by publication. Of the 825 posters, 198 (24%) poster presentations were ultimately published in 1 of 32 medical journals within a weighted mean of 17.6 months. Of the 32 journals, 25 (78.1%) represented peer-reviewed journals. The publication rate of poster presentations at the ACFAS ASC was less than that of oral manuscripts presented at the same meeting during the same period and was also less than the orthopedic subspecialty poster presentation publication rates. Therefore, attendees of the ACFAS ASC should be aware that only a few of the posters presented at the ACFAS ASC will be valid because they will not survive the rigors of publication 76% of the time. Additionally, more stringent selection criteria should be used so that the selected poster presentations can ultimately withstand the publication process. PMID:22196458

  14. An Extensive Evaluation of Read Trimming Effects on Illumina NGS Data Analysis

    PubMed Central

    Morgante, Michele; Giorgi, Federico M.

    2013-01-01

    Next Generation Sequencing is having an extremely strong impact in biological and medical research and diagnostics, with applications ranging from gene expression quantification to genotyping and genome reconstruction. Sequencing data is often provided as raw reads which are processed prior to analysis 1 of the most used preprocessing procedures is read trimming, which aims at removing low quality portions while preserving the longest high quality part of a NGS read. In the current work, we evaluate nine different trimming algorithms in four datasets and three common NGS-based applications (RNA-Seq, SNP calling and genome assembly). Trimming is shown to increase the quality and reliability of the analysis, with concurrent gains in terms of execution time and computational resources needed. PMID:24376861

  15. CANARY: the on-sky NGS/LGS MOAO demonstrator for EAGLE

    NASA Astrophysics Data System (ADS)

    Myers, Richard M.; Hubert, Zoltán; Morris, Timothy J.; Gendron, Eric; Dipper, Nigel A.; Kellerer, Aglaé; Goodsell, Stephen J.; Rousset, Gérard; Younger, Eddy; Marteaud, Michel; Basden, Alastair G.; Chemla, Fanny; Guzman, C. Dani; Fusco, Thierry; Geng, Deli; Le Roux, Brice; Harrison, Mark A.; Longmore, Andrew J.; Young, Laura K.; Vidal, Fabrice; Greenaway, Alan H.

    2008-07-01

    EAGLE is a multi-object 3D spectroscopy instrument currently under design for the 42-metre European Extremely Large Telescope (E-ELT). Precise requirements are still being developed, but it is clear that EAGLE will require (~100 x 100 actuator) adaptive optics correction of ~20 - 60 spectroscopic subfields distributed across a ~5 arcminute diameter field of view. It is very likely that LGS will be required to provide wavefront sensing with the necessary sky coverage. Two alternative adaptive optics implementations are being considered, one of which is Multi-Object Adaptive Optics (MOAO). In this scheme, wavefront tomography is performed using a set of LGS and NGS in either a completely open-loop manner, or in a configuration that is only closed-loop with respect to only one DM, probably the adaptive M4 of the E-ELT. The fine wavefront correction required for each subfield is then applied in a completely open-loop fashion by independent DMs within each separate optical relay. The novelty of this scheme is such that on-sky demonstration is required prior to final construction of an E-ELT instrument. The CANARY project will implement a single channel of an MOAO system on the 4.2m William Herschel Telescope. This will be a comprehensive demonstration, which will be phased to include pure NGS, low-order NGS-LGS and high-order woofer-tweeter NGS-LGS configurations. The LGSs used for these demonstrations will be Rayleigh systems, where the variable range-gate height and extension can be used to simulate many of the LGS effects on the E-ELT. We describe the requirements for the various phases of MOAO demonstration, the corresponding CANARY configurations and capabilities and the current conceptual designs of the various subsystems.

  16. Environmental Performance Report 2013: Annual Site Environmental Report per the U.S. Department of Energy Order 231.1-1B (Management Publication)

    SciTech Connect

    Schlomberg, K.; Eickhoff, J.; Beatty, B.; Braus, G.; Durbin, L.; Fiehweg, R.; Ray, M.; Ryon, T.; Schmitz, E.

    2014-08-01

    The National Renewable Energy Laboratory's (NREL's) Environmental Performance Report provides a description of the laboratory's environmental management activities for 2013, including information on environmental and sustainability performance, environmental compliance activities and status, and environmental protection programs, highlights, and successes. The purpose of this report is to ensure that U.S. Department of Energy (DOE) and the public receive timely, accurate information about events that have affected or could adversely affect the health, safety, and security of the public or workers; the environment; or the operations of DOE facilities. This report meets the requirements of the Annual Site Environmental Report and is prepared in accordance with the DOE Order 231.1B, Environment, Safety and Health Reporting.

  17. NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data

    PubMed Central

    Patel, Ravi K.; Jain, Mukesh

    2012-01-01

    Next generation sequencing (NGS) technologies provide a high-throughput means to generate large amount of sequence data. However, quality control (QC) of sequence data generated from these technologies is extremely important for meaningful downstream analysis. Further, highly efficient and fast processing tools are required to handle the large volume of datasets. Here, we have developed an application, NGS QC Toolkit, for quality check and filtering of high-quality data. This toolkit is a standalone and open source application freely available at http://www.nipgr.res.in/ngsqctoolkit.html. All the tools in the application have been implemented in Perl programming language. The toolkit is comprised of user-friendly tools for QC of sequencing data generated using Roche 454 and Illumina platforms, and additional tools to aid QC (sequence format converter and trimming tools) and analysis (statistics tools). A variety of options have been provided to facilitate the QC at user-defined parameters. The toolkit is expected to be very useful for the QC of NGS data to facilitate better downstream analysis. PMID:22312429

  18. Functional genomics of tomato: opportunities and challenges in post-genome NGS era.

    PubMed

    Kumar, Rahul; Khurana, Ashima

    2014-12-01

    The Tomato Genome Sequencing Project represented a landmark venture in the history of sequencing projects where both Sanger's and next-generation sequencing (NGS) technologies were employed, and a highly accurate and one of the best assembled plant genomes along with a draft of the wild relative, Solanum pimpinellifolium, were released in 2012. However, the functional potential of the major portion of this newly generated resource is still undefined. The very first challenge before scientists working on tomato functional biology is to exploit this high-quality reference sequence for tapping of the wealth of genetic variants for improving agronomic traits in cultivated tomatoes. The sequence data generated recently by 150 Tomato Genome Consortium would further uncover the natural alleles present in different tomato genotypes. Therefore, we found it relevant to have a fresh outlook on tomato functional genomics in the context of application of NGS technologies in its post-genome sequencing phase. Herein, we provide an overview how NGS technologies vis-a-vis available reference sequence have assisted each other for their mutual improvement and how their combined use could further facilitate the development of other 'omics' tools, required to propel the Solanaceae research. Additionally, we highlight the challenges associated with the application of these cutting-edge technologies. PMID:25431420

  19. tropiTree: an NGS-based EST-SSR resource for 24 tropical tree species.

    PubMed

    Russell, Joanne R; Hedley, Peter E; Cardle, Linda; Dancey, Siobhan; Morris, Jenny; Booth, Allan; Odee, David; Mwaura, Lucy; Omondi, William; Angaine, Peter; Machua, Joseph; Muchugi, Alice; Milne, Iain; Kindt, Roeland; Jamnadass, Ramni; Dawson, Ian K

    2014-01-01

    The development of genetic tools for non-model organisms has been hampered by cost, but advances in next-generation sequencing (NGS) have created new opportunities. In ecological research, this raises the prospect for developing molecular markers to simultaneously study important genetic processes such as gene flow in multiple non-model plant species within complex natural and anthropogenic landscapes. Here, we report the use of bar-coded multiplexed paired-end Illumina NGS for the de novo development of expressed sequence tag-derived simple sequence repeat (EST-SSR) markers at low cost for a range of 24 tree species. Each chosen tree species is important in complex tropical agroforestry systems where little is currently known about many genetic processes. An average of more than 5,000 EST-SSRs was identified for each of the 24 sequenced species, whereas prior to analysis 20 of the species had fewer than 100 nucleotide sequence citations. To make results available to potential users in a suitable format, we have developed an open-access, interactive online database, tropiTree (http://bioinf.hutton.ac.uk/tropiTree), which has a range of visualisation and search facilities, and which is a model for the efficient presentation and application of NGS data. PMID:25025376

  20. tropiTree: An NGS-Based EST-SSR Resource for 24 Tropical Tree Species

    PubMed Central

    Russell, Joanne R.; Hedley, Peter E.; Cardle, Linda; Dancey, Siobhan; Morris, Jenny; Booth, Allan; Odee, David; Mwaura, Lucy; Omondi, William; Angaine, Peter; Machua, Joseph; Muchugi, Alice; Milne, Iain; Kindt, Roeland; Jamnadass, Ramni; Dawson, Ian K.

    2014-01-01

    The development of genetic tools for non-model organisms has been hampered by cost, but advances in next-generation sequencing (NGS) have created new opportunities. In ecological research, this raises the prospect for developing molecular markers to simultaneously study important genetic processes such as gene flow in multiple non-model plant species within complex natural and anthropogenic landscapes. Here, we report the use of bar-coded multiplexed paired-end Illumina NGS for the de novo development of expressed sequence tag-derived simple sequence repeat (EST-SSR) markers at low cost for a range of 24 tree species. Each chosen tree species is important in complex tropical agroforestry systems where little is currently known about many genetic processes. An average of more than 5,000 EST-SSRs was identified for each of the 24 sequenced species, whereas prior to analysis 20 of the species had fewer than 100 nucleotide sequence citations. To make results available to potential users in a suitable format, we have developed an open-access, interactive online database, tropiTree (http://bioinf.hutton.ac.uk/tropiTree), which has a range of visualisation and search facilities, and which is a model for the efficient presentation and application of NGS data. PMID:25025376

  1. Refining Our Mission: Continuing Education's Role in Engagement, Outreach and Public Service. Proceedings of the Annual Meeting of the Association for Continuing Higher Education (69th, Roanoke, VA, October 27-30, 2007)

    ERIC Educational Resources Information Center

    Barrineau, Irene T., Ed.

    2007-01-01

    The 2007 Annual Meeting of the Association for Continuing Higher Education was themed "Refining Our Mission: Continuing Education's Role in Engagement, Outreach and Public Service." Opportunities were available to participate in sessions relating to outreach, partnerships and public service and perspectives on this theme were presented by the…

  2. Funding Public Higher Education for Results: Fad or Trend? Results from the Second Annual Survey. Rockefeller Reports.

    ERIC Educational Resources Information Center

    Burke, Joseph C.; Serban, Andreea M.

    This report presents the results of a telephone survey conducted in 1997 and 1998 of the 50 State Higher Education Finance Officers in regard to performance budgeting and performance funding of public higher education. It was found that 21 states used some form of performance budgeting in public higher education in 1998, up from 16 in 1997, and…

  3. Indispensable Resources for Institutional Researchers: An Analysis of AIR Publication Topics Since 1974. AIR 1997 Annual Forum Paper.

    ERIC Educational Resources Information Center

    Volkwein, J. Fredericks

    This study identified and classified the Association for Institutional Research (AIR) publication topics and authors since 1973 in order to provide a reference resource for institutional researchers. The study reviewed the following AIR publication collections: "Research in Higher Education"; "New Directions for Institutional Research"; "Resources…

  4. Betting on Teachers: The 43rd Annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes toward the Public Schools

    ERIC Educational Resources Information Center

    Bushaw, William J.; Lopez, Shane J.

    2011-01-01

    This is the latest in a series of polls sponsored by Phi Delta Kappa International with the Gallup organization. Some important findings of this year's poll include: About half of us believe teacher unions are hurting public education, but we're more likely to support teacher union leaders than governors in disputes over teacher collective…

  5. 78 FR 4378 - Annual List of Newspapers To Be Used by the Alaska Region for Publication of Legal Notices of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ... appeal under 36 CFR Part 215, thereby allowing them to receive constructive notice of a decision or... the appeals process. DATES: Publication of legal notices in the listed newspapers begins on February...

  6. The Tsimane’ Amazonian Panel Study (TAPS): Nine years (2002-2010) of annual data available to the public

    PubMed Central

    Leonard, William R.; Reyes-García, Victoria; Tanner, Susan; Rosinger, Asher; Schultz, Alan; Vadez, Vincent; Zhang, Rebecca; Godoy, Ricardo

    2016-01-01

    This brief communication contains a description of the 2002-2010 annual panel collected by the Tsimane’ Amazonian Panel Study team. The study took place among the Tsimane’, a native Amazonian society of forager-horticulturalists. The team tracked a wide range of socio-economic and anthropometric variables from all residents (633 adults ≥16 years; 820 children) in 13 villages along the Maniqui River, department of Beni. The panel is ideally suited to examine how market exposure and modernization affect the well-being of a highly autarkic population and to examine human growth in a non-Western rural setting. PMID:26280812

  7. The Tsimane' Amazonian Panel Study (TAPS): Nine years (2002-2010) of annual data available to the public.

    PubMed

    Leonard, William R; Reyes-García, Victoria; Tanner, Susan; Rosinger, Asher; Schultz, Alan; Vadez, Vincent; Zhang, Rebecca; Godoy, Ricardo

    2015-12-01

    This brief communication contains a description of the 2002-2010 annual panel collected by the Tsimane' Amazonian Panel Study team. The study took place among the Tsimane', a native Amazonian society of forager-horticulturalists. The team tracked a wide range of socio-economic and anthropometric variables from all residents (633 adults ≥16 years; 820 children) in 13 villages along the Maniqui River, Department of Beni. The panel is ideally suited to examine how market exposure and modernization affect the well-being of a highly autarkic population and to examine human growth in a non-Western rural setting. PMID:26280812

  8. 26 CFR 301.6104(d)-1 - Public inspection and distribution of applications for tax exemption and annual information...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Public inspection and distribution of... Section 301.6104(d)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED... recognition of exemption under section 501, it shall make its application for tax exemption (as defined...

  9. Michigan Council on Physical Fitness and Health Annual Report to the Michigan Department of Public Health, December 29, 1978.

    ERIC Educational Resources Information Center

    Michigan State Dept. of Public Health, Lansing. Council on Physical Fitness & Health.

    The projects which the Michigan Council on Physical Fitness and Health undertook in 1978 are reported and described here. The projects and activities include: a school physical fitness program that provided assistance to public, private, and parochial schools in monitoring the physical fitness levels of all students in grades kindergarten through…

  10. Implementation of Public Law 94-142: The Education for All Handicapped Children Act. Sixth Annual Report to Congress.

    ERIC Educational Resources Information Center

    Special Education Programs (ED/OSERS), Washington, DC. Div. of Educational Services.

    The report examines progress for the school years 1981-82 and 1982-83 in implementing P.L. 94-142, The Education for All Handicapped Children Act. Following an executive summary, an analysis of four major aspects of the law's implementation is detailed. A section on students receiving a free appropriate public education cites data on the number of…

  11. 75 FR 61239 - Request for Public Comments on Annual Review of Country Eligibility for Benefits Under the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-04

    ... Subcommittee of the Trade Policy Staff Committee (the ``Subcommittee'') is requesting written public comments..., please contact Gloria Blue, Executive Secretary, Trade Policy Staff Committee, at (202) 395-6143 to make...-0024. Carmen Suro-Bredie, Chairman, Trade Policy Staff Committee. BILLING CODE 3190-W0-P...

  12. Excellence in Higher Education as Defined by Legislators and Public and Private Institutions Administrators. AIR 1991 Annual Forum Paper.

    ERIC Educational Resources Information Center

    Munoz, Grisel; And Others

    This report presents the results of a delphi study used to determine the criteria for defining excellence in higher education. An open questionnaire was administered to the commissions on education of the Puerto Rico Legislature, the administrators and governing boards of the major public and private postsecondary institutions in Puerto Rico, and…

  13. State Financial Control Practices and Public Universities: Results of a National Study. Revised. ASHE 1984 Annual Meeting Paper.

    ERIC Educational Resources Information Center

    Volkwein, J. Fredericks

    Results of a national study of budgetary control imposed upon 88 Ph.D. granting public universities by 49 state governments (excluding Alaska) are presented. A comparative analysis is provided of financial control practices in each state, along with an index that places these practices along a continuum. Information is included on the nature and…

  14. 26 CFR 301.6104(d)-1 - Public inspection and distribution of applications for tax exemption and annual information...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... day care, health care or scientific or medical research); and (B) The site does not serve as an office... days from the date it receives the request, notify the requester of its prepayment policy and the... 26 Internal Revenue 18 2014-04-01 2014-04-01 false Public inspection and distribution...

  15. 26 CFR 301.6104(d)-1 - Public inspection and distribution of applications for tax exemption and annual information...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... day care, health care or scientific or medical research); and (B) The site does not serve as an office... days from the date it receives the request, notify the requester of its prepayment policy and the... 26 Internal Revenue 18 2013-04-01 2013-04-01 false Public inspection and distribution...

  16. 26 CFR 301.6104(d)-1 - Public inspection and distribution of applications for tax exemption and annual information...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... day care, health care or scientific or medical research); and (B) The site does not serve as an office... days from the date it receives the request, notify the requester of its prepayment policy and the... 26 Internal Revenue 18 2012-04-01 2012-04-01 false Public inspection and distribution...

  17. The Implications for Higher-Accuracy Absolute Measurements for NGS and its GRAV-D Project

    NASA Astrophysics Data System (ADS)

    Childers, V. A.; Winester, D.; Roman, D. R.; Eckl, M. C.; Smith, D. A.

    2013-12-01

    Absolute and relative gravity measurements play an important role in the work of NOAA's National Geodetic Survey (NGS). When NGS decided to replace the US national vertical datum, the Gravity for the Redefinition of the American Vertical Datum (GRAV-D) project added a new dimension to the NGS gravity program. Airborne gravity collection would complement existing satellite and surface gravity data to allow the creation of a gravimetric geoid sufficiently accurate to form the basis of the new reference surface. To provide absolute gravity ties for the airborne surveys, initially new FG5 absolute measurements were made at existing absolute stations and relative measurements were used to transfer those measurements to excenters near the absolute mark and to the aircraft sensor height at the parking space. In 2011, NGS obtained a field-capable A10 absolute gravimeter from Micro-g LaCoste which became the basis of the support of the airborne surveys. Now A10 measurements are made at the aircraft location and transferred to sensor height. Absolute and relative gravity play other roles in GRAV-D. Comparison of surface data with new airborne collection will highlight surface surveys with bias or tilt errors and can provide enough information to repair or discard the data. We expect that areas of problem surface data may be re-measured. The GRAV-D project also plans to monitor the geoid in regions of rapid change and update the vertical datum when appropriate. Geoid change can result from glacial isostatic adjustment (GIA), tectonic change, and the massive drawdown of large scale aquifers. The NGS plan for monitoring these changes over time is still in its preliminary stages and is expected to rely primarily on the GRACE and GRACE Follow On satellite data in conjunction with models of GIA and tectonic change. We expect to make absolute measurements in areas of rapid change in order to verify model predictions. With the opportunities presented by rapid, highly accurate

  18. New genes and pathomechanisms in mitochondrial disorders unraveled by NGS technologies.

    PubMed

    Legati, Andrea; Reyes, Aurelio; Nasca, Alessia; Invernizzi, Federica; Lamantea, Eleonora; Tiranti, Valeria; Garavaglia, Barbara; Lamperti, Costanza; Ardissone, Anna; Moroni, Isabella; Robinson, Alan; Ghezzi, Daniele; Zeviani, Massimo

    2016-08-01

    Next Generation Sequencing (NGS) technologies are revolutionizing the diagnostic screening for rare disease entities, including primary mitochondrial disorders, particularly those caused by nuclear gene defects. NGS approaches are able to identify the causative gene defects in small families and even single individuals, unsuitable for investigation by traditional linkage analysis. These technologies are contributing to fill the gap between mitochondrial disease cases defined on the basis of clinical, neuroimaging and biochemical readouts, which still outnumber by approximately 50% the cases for which a molecular-genetic diagnosis is attained. We have been using a combined, two-step strategy, based on targeted genes panel as a first NGS screening, followed by whole exome sequencing (WES) in still unsolved cases, to analyze a large cohort of subjects, that failed to show mutations in mtDNA and in ad hoc sets of specific nuclear genes, sequenced by the Sanger's method. Not only this approach has allowed us to reach molecular diagnosis in a significant fraction (20%) of these difficult cases, but it has also revealed unexpected and conceptually new findings. These include the possibility of marked variable penetrance of recessive mutations, the identification of large-scale DNA rearrangements, which explain spuriously heterozygous cases, and the association of mutations in known genes with unusual, previously unreported clinical phenotypes. Importantly, WES on selected cases has unraveled the presence of pathogenic mutations in genes encoding non-mitochondrial proteins (e.g. the transcription factor E4F1), an observation that further expands the intricate genetics of mitochondrial disease and suggests a new area of investigation in mitochondrial medicine. This article is part of a Special Issue entitled 'EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2-6, 2016', edited by Prof. Paolo Bernardi. PMID:26968897

  19. Sample Results From The Extraction, Scrub, And Strip Test For The Blended NGS Solvent

    SciTech Connect

    Washington, A. L. II; Peters, T. B.

    2014-03-03

    This report summarizes the results of the extraction, scrub, and strip testing for the September 2013 sampling of the Next Generation Solvent (NGS) Blended solvent from the Modular Caustic Side-Solvent Extraction Unit (MCU) Solvent Hold Tank. MCU is in the process of transitioning from the BOBCalixC6 solvent to the NGS Blend solvent. As part of that transition, MCU has intentionally created a blended solvent to be processed using the Salt Batch program. This sample represents the first sample received from that blended solvent. There were two ESS tests performed where NGS blended solvent performance was assessed using either the Tank 21 material utilized in the Salt Batch 7 analyses or a simulant waste material used in the V-5/V-10 contactor testing. This report tabulates the temperature corrected cesium distribution, or DCs values, step recovery percentage, and actual temperatures recorded during the experiment. This report also identifies the sample receipt date, preparation method, and analysis performed in the accumulation of the listed values. The calculated extraction DCs values using the Tank 21H material and simulant are 59.4 and 53.8, respectively. The DCs values for two scrub and three strip processes for the Tank 21 material are 4.58, 2.91, 0.00184, 0.0252, and 0.00575, respectively. The D-values for two scrub and three strip processes for the simulant are 3.47, 2.18, 0.00468, 0.00057, and 0.00572, respectively. These values are similar to previous measurements of Salt Batch 7 feed with lab-prepared blended solvent. These numbers are considered compatible to allow simulant testing to be completed in place of actual waste due to the limited availability of feed material.

  20. Precise GPS ephemerides from DMA and NGS tested by time transfer

    NASA Technical Reports Server (NTRS)

    Lewandowski, Wlodzimierz W.; Petit, Gerard; Thomas, Claudine

    1992-01-01

    It was shown that the use of the Defense Mapping Agency's (DMA) precise ephemerides brings a significant improvement to the accuracy of GPS time transfer. At present a new set of precise ephemerides produced by the National Geodetic Survey (NGS) has been made available to the timing community. This study demonstrates that both types of precise ephemerides improve long-distance GPS time transfer and remove the effects of Selective Availability (SA) degradation of broadcast ephemerides. The issue of overcoming SA is also discussed in terms of the routine availability of precise ephemerides.

  1. Unipro UGENE NGS pipelines and components for variant calling, RNA-seq and ChIP-seq data analyses

    PubMed Central

    Golosova, Olga; Henderson, Ross; Vaskin, Yuriy; Gabrielian, Andrei; Grekhov, German; Oler, Andrew J.; Quiñones, Mariam; Hurt, Darrell; Fursov, Mikhail; Huyen, Yentram

    2014-01-01

    The advent of Next Generation Sequencing (NGS) technologies has opened new possibilities for researchers. However, the more biology becomes a data-intensive field, the more biologists have to learn how to process and analyze NGS data with complex computational tools. Even with the availability of common pipeline specifications, it is often a time-consuming and cumbersome task for a bench scientist to install and configure the pipeline tools. We believe that a unified, desktop and biologist-friendly front end to NGS data analysis tools will substantially improve productivity in this field. Here we present NGS pipelines “Variant Calling with SAMtools”, “Tuxedo Pipeline for RNA-seq Data Analysis” and “Cistrome Pipeline for ChIP-seq Data Analysis” integrated into the Unipro UGENE desktop toolkit. We describe the available UGENE infrastructure that helps researchers run these pipelines on different datasets, store and investigate the results and re-run the pipelines with the same parameters. These pipeline tools are included in the UGENE NGS package. Individual blocks of these pipelines are also available for expert users to create their own advanced workflows. PMID:25392756

  2. Next generation sequencing (NGS) database for tandem repeats with multiple pattern 2°-shaft multicore string matching.

    PubMed

    Someswara Rao, Chinta; Raju, S Viswanadha

    2016-03-01

    Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research in recent years. To provide the comprehensive NGS resource for the research, in this paper , we have considered 10 loci/codi/repeats TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA. Then we developed the NGS Tandem Repeat Database (TandemRepeatDB) for all the chromosomes of Homo sapiens, Callithrix jacchus, Chlorocebus sabaeus, Gorilla gorilla, Macaca fascicularis, Macaca mulatta, Nomascus leucogenys, Pan troglodytes, Papio anubis and Pongo abelii genome data sets for all those locis. We find the successive occurence frequency for all the above 10 SSR (simple sequence repeats) in the above genome data sets on a chromosome-by-chromosome basis with multiple pattern 2° shaft multicore string matching. PMID:26981434

  3. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    SciTech Connect

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-end NGS analysis requirements. The Globus Genomics system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.

  4. A case study for cloud based high throughput analysis of NGS data using the globus genomics system.

    PubMed

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the "Globus Genomics" system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-endNGS analysis requirements. The Globus Genomics system is built on Amazon 's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research. PMID:26925205

  5. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    DOE PAGESBeta

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-end NGS analysis requirements. The Globus Genomicsmore » system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.« less

  6. Next generation sequencing (NGS) database for tandem repeats with multiple pattern 2°-shaft multicore string matching

    PubMed Central

    Someswara Rao, Chinta; Raju, S. Viswanadha

    2016-01-01

    Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research in recent years. To provide the comprehensive NGS resource for the research, in this paper , we have considered 10 loci/codi/repeats TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA. Then we developed the NGS Tandem Repeat Database (TandemRepeatDB) for all the chromosomes of Homo sapiens, Callithrix jacchus, Chlorocebus sabaeus, Gorilla gorilla, Macaca fascicularis, Macaca mulatta, Nomascus leucogenys, Pan troglodytes, Papio anubis and Pongo abelii genome data sets for all those locis. We find the successive occurence frequency for all the above 10 SSR (simple sequence repeats) in the above genome data sets on a chromosome-by-chromosome basis with multiple pattern 2° shaft multicore string matching. PMID:26981434

  7. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    PubMed Central

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2014-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-endNGS analysis requirements. The Globus Genomics system is built on Amazon 's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research. PMID:26925205

  8. NGS-Based Assay for the Identification of Individuals Carrying Recessive Genetic Mutations in Reproductive Medicine.

    PubMed

    Abulí, Anna; Boada, Montserrat; Rodríguez-Santiago, Benjamín; Coroleu, Buenaventura; Veiga, Anna; Armengol, Lluís; Barri, Pedro N; Pérez-Jurado, Luis A; Estivill, Xavier

    2016-06-01

    Next-generation sequencing (NGS) has the capacity of carrier screening in gamete donation (GD) programs. We have developed and validated an NGS carrier-screening test (qCarrier test) that includes 200 genes associated with 368 disorders (277 autosomal recessive and 37 X-linked). Carrier screening is performed on oocyte donation candidates and the male partner of oocyte recipient. Carriers of X-linked conditions are excluded from the GD program, whereas donors are chosen who do not carry mutations for the same gene/disease as the recipients. The validation phase showed a high sensitivity (>99% sensitivity) detecting all single-nucleotide variants, 13 indels, and 25 copy-number variants included in the validation set. A total of 1,301 individuals were analysed with the qCarrier test, including 483 candidate oocyte donors and 635 receptor couples, 105 females receiving sperm donation, and 39 couples seeking pregnancy. We identified 56% of individuals who are carriers for at least one genetic condition and 1.7% of female donors who were excluded from the program due to a carrier state of X-linked conditions. Globally, 3% of a priori assigned donations had a high reproductive risk that could be minimized after testing. Genetic counselling at different stages is essential for helping to facilitate a successful and healthy pregnancy. PMID:26990548

  9. NGS-based Molecular diagnosis of 105 eyeGENE® probands with Retinitis Pigmentosa

    PubMed Central

    Ge, Zhongqi; Bowles, Kristen; Goetz, Kerry; Scholl, Hendrik P. N.; Wang, Feng; Wang, Xinjing; Xu, Shan; Wang, Keqing; Wang, Hui; Chen, Rui

    2015-01-01

    The National Ophthalmic Disease Genotyping and Phenotyping Network (eyeGENE®) was established in an effort to facilitate basic and clinical research of human inherited eye disease. In order to provide high quality genetic testing to eyeGENE®’s enrolled patients which potentially aids clinical diagnosis and disease treatment, we carried out a pilot study and performed Next-generation sequencing (NGS) based molecular diagnosis for 105 Retinitis Pigmentosa (RP) patients randomly selected from the network. A custom capture panel was designed, which incorporated 195 known retinal disease genes, including 61 known RP genes. As a result, disease-causing mutations were identified in 52 out of 105 probands (solving rate of 49.5%). A total of 82 mutations were identified, and 48 of them were novel. Interestingly, for three probands the molecular diagnosis was inconsistent with the initial clinical diagnosis, while for five probands the molecular information suggested a different inheritance model other than that assigned by the physician. In conclusion, our study demonstrated that NGS target sequencing is efficient and sufficiently precise for molecular diagnosis of a highly heterogeneous patient cohort from eyeGENE®. PMID:26667666

  10. Assessment of the latest NGS enrichment capture methods in clinical context.

    PubMed

    García-García, Gema; Baux, David; Faugère, Valérie; Moclyn, Mélody; Koenig, Michel; Claustres, Mireille; Roux, Anne-Françoise

    2016-01-01

    Enrichment capture methods for NGS are widely used, however, they evolve rapidly and it is necessary to periodically measure their strengths and weaknesses before transfer to diagnostic services. We assessed two recently released custom DNA solution-capture enrichment methods for NGS, namely Illumina NRCCE and Agilent SureSelect(QXT), against a reference method NimbleGen SeqCap EZ Choice on a similar gene panel, sharing 678 kb and 110 genes. Two Illumina MiSeq runs of 12 samples each have been performed, for each of the three methods, using the same 24 patients (affected with sensorineural disorders). Technical outcomes have been computed and compared, including depth and evenness of coverage, enrichment in targeted regions, performance in GC-rich regions and ability to generate consistent variant datasets. While we show that the three methods resulted in suitable datasets for standard DNA variant discovery, we describe significant differences between the results for the above parameters. NimbleGen offered the best depth of coverage and evenness, while NRCCE showed the highest on target levels but high duplicate rates. SureSelect(QXT) showed an overall quality close to that of NimbleGen. The new methods exhibit reduced preparation time but behave differently. These findings will guide laboratories in their choice of library enrichment approach. PMID:26864517

  11. Assessment of the latest NGS enrichment capture methods in clinical context

    PubMed Central

    García-García, Gema; Baux, David; Faugère, Valérie; Moclyn, Mélody; Koenig, Michel; Claustres, Mireille; Roux, Anne-Françoise

    2016-01-01

    Enrichment capture methods for NGS are widely used, however, they evolve rapidly and it is necessary to periodically measure their strengths and weaknesses before transfer to diagnostic services. We assessed two recently released custom DNA solution-capture enrichment methods for NGS, namely Illumina NRCCE and Agilent SureSelectQXT, against a reference method NimbleGen SeqCap EZ Choice on a similar gene panel, sharing 678 kb and 110 genes. Two Illumina MiSeq runs of 12 samples each have been performed, for each of the three methods, using the same 24 patients (affected with sensorineural disorders). Technical outcomes have been computed and compared, including depth and evenness of coverage, enrichment in targeted regions, performance in GC-rich regions and ability to generate consistent variant datasets. While we show that the three methods resulted in suitable datasets for standard DNA variant discovery, we describe significant differences between the results for the above parameters. NimbleGen offered the best depth of coverage and evenness, while NRCCE showed the highest on target levels but high duplicate rates. SureSelectQXT showed an overall quality close to that of NimbleGen. The new methods exhibit reduced preparation time but behave differently. These findings will guide laboratories in their choice of library enrichment approach. PMID:26864517

  12. Panel-based NGS Reveals Novel Pathogenic Mutations in Autosomal Recessive Retinitis Pigmentosa

    PubMed Central

    Perez-Carro, Raquel; Corton, Marta; Sánchez-Navarro, Iker; Zurita, Olga; Sanchez-Bolivar, Noelia; Sánchez-Alcudia, Rocío; Lelieveld, Stefan H.; Aller, Elena; Lopez-Martinez, Miguel Angel; López-Molina, Mª Isabel; Fernandez-San Jose, Patricia; Blanco-Kelly, Fiona; Riveiro-Alvarez, Rosa; Gilissen, Christian; Millan, Jose M; Avila-Fernandez, Almudena; Ayuso, Carmen

    2016-01-01

    Retinitis pigmentosa (RP) is a group of inherited progressive retinal dystrophies (RD) characterized by photoreceptor degeneration. RP is highly heterogeneous both clinically and genetically, which complicates the identification of causative genes and mutations. Targeted next-generation sequencing (NGS) has been demonstrated to be an effective strategy for the detection of mutations in RP. In our study, an in-house gene panel comprising 75 known RP genes was used to analyze a cohort of 47 unrelated Spanish families pre-classified as autosomal recessive or isolated RP. Disease-causing mutations were found in 27 out of 47 cases achieving a mutation detection rate of 57.4%. In total, 33 pathogenic mutations were identified, 20 of which were novel mutations (60.6%). Furthermore, not only single nucleotide variations but also copy-number variations, including three large deletions in the USH2A and EYS genes, were identified. Finally seven out of 27 families, displaying mutations in the ABCA4, RP1, RP2 and USH2A genes, could be genetically or clinically reclassified. These results demonstrate the potential of our panel-based NGS strategy in RP diagnosis. PMID:26806561

  13. Characterization of oxides on Bruce A NGS liner tubes and steam generator tubes

    SciTech Connect

    Miller, D.G.; Burrill, K.A.

    1998-12-31

    Oxide deposits on end-fitting liner tubes and steam generator tubes from the Bruce A Nuclear Generating Station (NGS) were characterized in advance of the decontamination of the heat transport system (HTS) of Bruce Unit 2. Oxide loadings, and Co-60 surface activities and specific activities were determined for the oxides on inlet and outlet end-fitting liner tubes from Bruce Unit l, Bruce Unit 2 and Bruce Unit 4. Oxides on the inner surfaces of steam generator tubes from Bruce NGS Units 1 and 2 were also characterized. The consistency in the deposit characteristics on the inlet liner tubes and steam generator tubes from Bruce A, along with the absence of magnetite on the outlet liner tubes has led to the development of a model for iron transport in the HTS of pressurized heavy water reactors (PHWRs). The activity transport/fouling mechanism involves flow-accelerated corrosion of the outlet feeder pipes, followed by deposition of iron in the steam generators, along the inlet feeder pipes, on the inlet end fittings, on the inlet fuel bundles and on the inlet region of the pressure tube. The results of loop experiments using decontamination solutions indicated that the oxide was rapidly removed from inlet liner tubes. However, removal of the Cr-rich oxide from the outlet liner tubes was less efficient, requiring the Alkaline Permangante (AP) oxidizing pre-treatment that is typically used in light water reactors (LWRs). The steam generator tubes were effectively decontaminated.

  14. Integrated Systems for NGS Data Management and Analysis: Open Issues and Available Solutions

    PubMed Central

    Bianchi, Valerio; Ceol, Arnaud; Ogier, Alessandro G. E.; de Pretis, Stefano; Galeota, Eugenia; Kishore, Kamal; Bora, Pranami; Croci, Ottavio; Campaner, Stefano; Amati, Bruno; Morelli, Marco J.; Pelizzola, Mattia

    2016-01-01

    Next-generation sequencing (NGS) technologies have deeply changed our understanding of cellular processes by delivering an astonishing amount of data at affordable prices; nowadays, many biology laboratories have already accumulated a large number of sequenced samples. However, managing and analyzing these data poses new challenges, which may easily be underestimated by research groups devoid of IT and quantitative skills. In this perspective, we identify five issues that should be carefully addressed by research groups approaching NGS technologies. In particular, the five key issues to be considered concern: (1) adopting a laboratory management system (LIMS) and safeguard the resulting raw data structure in downstream analyses; (2) monitoring the flow of the data and standardizing input and output directories and file names, even when multiple analysis protocols are used on the same data; (3) ensuring complete traceability of the analysis performed; (4) enabling non-experienced users to run analyses through a graphical user interface (GUI) acting as a front-end for the pipelines; (5) relying on standard metadata to annotate the datasets, and when possible using controlled vocabularies, ideally derived from biomedical ontologies. Finally, we discuss the currently available tools in the light of these issues, and we introduce HTS-flow, a new workflow management system conceived to address the concerns we raised. HTS-flow is able to retrieve information from a LIMS database, manages data analyses through a simple GUI, outputs data in standard locations and allows the complete traceability of datasets, accompanying metadata and analysis scripts. PMID:27200084

  15. Integrated Systems for NGS Data Management and Analysis: Open Issues and Available Solutions.

    PubMed

    Bianchi, Valerio; Ceol, Arnaud; Ogier, Alessandro G E; de Pretis, Stefano; Galeota, Eugenia; Kishore, Kamal; Bora, Pranami; Croci, Ottavio; Campaner, Stefano; Amati, Bruno; Morelli, Marco J; Pelizzola, Mattia

    2016-01-01

    Next-generation sequencing (NGS) technologies have deeply changed our understanding of cellular processes by delivering an astonishing amount of data at affordable prices; nowadays, many biology laboratories have already accumulated a large number of sequenced samples. However, managing and analyzing these data poses new challenges, which may easily be underestimated by research groups devoid of IT and quantitative skills. In this perspective, we identify five issues that should be carefully addressed by research groups approaching NGS technologies. In particular, the five key issues to be considered concern: (1) adopting a laboratory management system (LIMS) and safeguard the resulting raw data structure in downstream analyses; (2) monitoring the flow of the data and standardizing input and output directories and file names, even when multiple analysis protocols are used on the same data; (3) ensuring complete traceability of the analysis performed; (4) enabling non-experienced users to run analyses through a graphical user interface (GUI) acting as a front-end for the pipelines; (5) relying on standard metadata to annotate the datasets, and when possible using controlled vocabularies, ideally derived from biomedical ontologies. Finally, we discuss the currently available tools in the light of these issues, and we introduce HTS-flow, a new workflow management system conceived to address the concerns we raised. HTS-flow is able to retrieve information from a LIMS database, manages data analyses through a simple GUI, outputs data in standard locations and allows the complete traceability of datasets, accompanying metadata and analysis scripts. PMID:27200084

  16. CDH1 mutations in gastric cancer patients from northern Brazil identified by Next- Generation Sequencing (NGS)

    PubMed Central

    El-Husny, Antonette; Raiol-Moraes, Milene; Amador, Marcos; Ribeiro-dos-Santos, André M.; Montagnini, André; Barbosa, Silvanira; Silva, Artur; Assumpção, Paulo; Ishak, Geraldo; Santos, Sidney; Pinto, Pablo; Cruz, Aline; Ribeiro-dos-Santos, Ândrea

    2016-01-01

    Abstract Gastric cancer is considered to be the fifth highest incident tumor worldwide and the third leading cause of cancer deaths. Developing regions report a higher number of sporadic cases, but there are only a few local studies related to hereditary cases of gastric cancer in Brazil to confirm this fact. CDH1 germline mutations have been described both in familial and sporadic cases, but there is only one recent molecular description of individuals from Brazil. In this study we performed Next Generation Sequencing (NGS) to assess CDH1 germline mutations in individuals who match the clinical criteria for Hereditary Diffuse Gastric Cancer (HDGC), or who exhibit very early diagnosis of gastric cancer. Among five probands we detected CDH1 germline mutations in two cases (40%). The mutation c.1023T > G was found in a HDGC family and the mutation c.1849G > A, which is nearly exclusive to African populations, was found in an early-onset case of gastric adenocarcinoma. The mutations described highlight the existence of gastric cancer cases caused by CDH1 germline mutations in northern Brazil, although such information is frequently ignored due to the existence of a large number of environmental factors locally. Our report represent the first CDH1 mutations in HDGC described from Brazil by an NGS platform. PMID:27192129

  17. CDH1 mutations in gastric cancer patients from northern Brazil identified by Next- Generation Sequencing (NGS).

    PubMed

    El-Husny, Antonette; Raiol-Moraes, Milene; Amador, Marcos; Ribeiro-Dos-Santos, André M; Montagnini, André; Barbosa, Silvanira; Silva, Artur; Assumpção, Paulo; Ishak, Geraldo; Santos, Sidney; Pinto, Pablo; Cruz, Aline; Ribeiro-Dos-Santos, Ândrea

    2016-05-13

    Gastric cancer is considered to be the fifth highest incident tumor worldwide and the third leading cause of cancer deaths. Developing regions report a higher number of sporadic cases, but there are only a few local studies related to hereditary cases of gastric cancer in Brazil to confirm this fact. CDH1 germline mutations have been described both in familial and sporadic cases, but there is only one recent molecular description of individuals from Brazil. In this study we performed Next Generation Sequencing (NGS) to assess CDH1 germline mutations in individuals who match the clinical criteria for Hereditary Diffuse Gastric Cancer (HDGC), or who exhibit very early diagnosis of gastric cancer. Among five probands we detected CDH1 germline mutations in two cases (40%). The mutation c.1023T > G was found in a HDGC family and the mutation c.1849G > A, which is nearly exclusive to African populations, was found in an early-onset case of gastric adenocarcinoma. The mutations described highlight the existence of gastric cancer cases caused by CDH1 germline mutations in northern Brazil, although such information is frequently ignored due to the existence of a large number of environmental factors locally. Our report represent the first CDH1 mutations in HDGC described from Brazil by an NGS platform. PMID:27192129

  18. Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities

    PubMed Central

    Tan, BoonFei; Ng, Charmaine; Nshimyimana, Jean Pierre; Loh, Lay Leng; Gin, Karina Y.-H.; Thompson, Janelle R.

    2015-01-01

    Water quality is an emergent property of a complex system comprised of interacting microbial populations and introduced microbial and chemical contaminants. Studies leveraging next-generation sequencing (NGS) technologies are providing new insights into the ecology of microbially mediated processes that influence fresh water quality such as algal blooms, contaminant biodegradation, and pathogen dissemination. In addition, sequencing methods targeting small subunit (SSU) rRNA hypervariable regions have allowed identification of signature microbial species that serve as bioindicators for sewage contamination in these environments. Beyond amplicon sequencing, metagenomic and metatranscriptomic analyses of microbial communities in fresh water environments reveal the genetic capabilities and interplay of waterborne microorganisms, shedding light on the mechanisms for production and biodegradation of toxins and other contaminants. This review discusses the challenges and benefits of applying NGS-based methods to water quality research and assessment. We will consider the suitability and biases inherent in the application of NGS as a screening tool for assessment of biological risks and discuss the potential and limitations for direct quantitative interpretation of NGS data. Secondly, we will examine case studies from recent literature where NGS based methods have been applied to topics in water quality assessment, including development of bioindicators for sewage pollution and microbial source tracking, characterizing the distribution of toxin and antibiotic resistance genes in water samples, and investigating mechanisms of biodegradation of harmful pollutants that threaten water quality. Finally, we provide a short review of emerging NGS platforms and their potential applications to the next generation of water quality assessment tools. PMID:26441948

  19. Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities.

    PubMed

    Tan, BoonFei; Ng, Charmaine; Nshimyimana, Jean Pierre; Loh, Lay Leng; Gin, Karina Y-H; Thompson, Janelle R

    2015-01-01

    Water quality is an emergent property of a complex system comprised of interacting microbial populations and introduced microbial and chemical contaminants. Studies leveraging next-generation sequencing (NGS) technologies are providing new insights into the ecology of microbially mediated processes that influence fresh water quality such as algal blooms, contaminant biodegradation, and pathogen dissemination. In addition, sequencing methods targeting small subunit (SSU) rRNA hypervariable regions have allowed identification of signature microbial species that serve as bioindicators for sewage contamination in these environments. Beyond amplicon sequencing, metagenomic and metatranscriptomic analyses of microbial communities in fresh water environments reveal the genetic capabilities and interplay of waterborne microorganisms, shedding light on the mechanisms for production and biodegradation of toxins and other contaminants. This review discusses the challenges and benefits of applying NGS-based methods to water quality research and assessment. We will consider the suitability and biases inherent in the application of NGS as a screening tool for assessment of biological risks and discuss the potential and limitations for direct quantitative interpretation of NGS data. Secondly, we will examine case studies from recent literature where NGS based methods have been applied to topics in water quality assessment, including development of bioindicators for sewage pollution and microbial source tracking, characterizing the distribution of toxin and antibiotic resistance genes in water samples, and investigating mechanisms of biodegradation of harmful pollutants that threaten water quality. Finally, we provide a short review of emerging NGS platforms and their potential applications to the next generation of water quality assessment tools. PMID:26441948

  20. Commissioner's Annual Report to the Education Committees of the Senate and General Assembly on Violence, Vandalism and Substance Abuse in New Jersey Public Schools, July 1, 2006 to June 30, 2007

    ERIC Educational Resources Information Center

    New Jersey Department of Education, 2008

    2008-01-01

    The "Commissioner of Education's Report on Violence, Vandalism and Substance Abuse in New Jersey Public Schools", submitted annually to the education committees of the Senate and Assembly, provides the Legislature with data in four broad incident categories: violence, vandalism, weapons, and substance abuse. This year's report highlights thee-year…

  1. Commissioner's Annual Report to the Education Committees of the Senate and General Assembly on Violence, Vandalism and Substance Abuse in New Jersey Public Schools, July 1, 2005 to June 30, 2006

    ERIC Educational Resources Information Center

    New Jersey Department of Education, 2007

    2007-01-01

    The Commissioner of Education's Report on Violence, Vandalism and Substance Abuse in New Jersey Public Schools, submitted annually to the education committees of the Senate and Assembly, provides the Legislature with data in four broad incident categories: violence, vandalism, weapons, and substance abuse. The report also summarizes initiatives…

  2. Commissioner's Annual Report to the Education Committees of the Senate and General Assembly on Violence, Vandalism and Substance Abuse in New Jersey Public Schools, July 1, 2004 to June 30, 2005

    ERIC Educational Resources Information Center

    New Jersey Department of Education, 2006

    2006-01-01

    The Commissioner of Education's Report on Violence, Vandalism and Substance Abuse in New Jersey Public Schools is submitted annually to the education committees of the Senate and Assembly of the New Jersey State Legislature. It provides the Legislature with data in four broad categories of incidents: violence, vandalism, weapons, and substance…

  3. RAP: RNA-Seq Analysis Pipeline, a new cloud-based NGS web application

    PubMed Central

    2015-01-01

    Background The study of RNA has been dramatically improved by the introduction of Next Generation Sequencing platforms allowing massive and cheap sequencing of selected RNA fractions, also providing information on strand orientation (RNA-Seq). The complexity of transcriptomes and of their regulative pathways make RNA-Seq one of most complex field of NGS applications, addressing several aspects of the expression process (e.g. identification and quantification of expressed genes and transcripts, alternative splicing and polyadenylation, fusion genes and trans-splicing, post-transcriptional events, etc.). Moreover, the huge volume of data generated by NGS platforms introduces unprecedented computational and technological challenges to efficiently analyze and store sequence data and results. Methods In order to provide researchers with an effective and friendly resource for analyzing RNA-Seq data, we present here RAP (RNA-Seq Analysis Pipeline), a cloud computing web application implementing a complete but modular analysis workflow. This pipeline integrates both state-of-the-art bioinformatics tools for RNA-Seq analysis and in-house developed scripts to offer to the user a comprehensive strategy for data analysis. RAP is able to perform quality checks (adopting FastQC and NGS QC Toolkit), identify and quantify expressed genes and transcripts (with Tophat, Cufflinks and HTSeq), detect alternative splicing events (using SpliceTrap) and chimeric transcripts (with ChimeraScan). This pipeline is also able to identify splicing junctions and constitutive or alternative polyadenylation sites (implementing custom analysis modules) and call for statistically significant differences in genes and transcripts expression, splicing pattern and polyadenylation site usage (using Cuffdiff2 and DESeq). Results Through a user friendly web interface, the RAP workflow can be suitably customized by the user and it is automatically executed on our cloud computing environment. This strategy

  4. MutAid: Sanger and NGS Based Integrated Pipeline for Mutation Identification, Validation and Annotation in Human Molecular Genetics

    PubMed Central

    Pandey, Ram Vinay; Pabinger, Stephan; Kriegner, Albert; Weinhäusel, Andreas

    2016-01-01

    Traditional Sanger sequencing as well as Next-Generation Sequencing have been used for the identification of disease causing mutations in human molecular research. The majority of currently available tools are developed for research and explorative purposes and often do not provide a complete, efficient, one-stop solution. As the focus of currently developed tools is mainly on NGS data analysis, no integrative solution for the analysis of Sanger data is provided and consequently a one-stop solution to analyze reads from both sequencing platforms is not available. We have therefore developed a new pipeline called MutAid to analyze and interpret raw sequencing data produced by Sanger or several NGS sequencing platforms. It performs format conversion, base calling, quality trimming, filtering, read mapping, variant calling, variant annotation and analysis of Sanger and NGS data under a single platform. It is capable of analyzing reads from multiple patients in a single run to create a list of potential disease causing base substitutions as well as insertions and deletions. MutAid has been developed for expert and non-expert users and supports four sequencing platforms including Sanger, Illumina, 454 and Ion Torrent. Furthermore, for NGS data analysis, five read mappers including BWA, TMAP, Bowtie, Bowtie2 and GSNAP and four variant callers including GATK-HaplotypeCaller, SAMTOOLS, Freebayes and VarScan2 pipelines are supported. MutAid is freely available at https://sourceforge.net/projects/mutaid. PMID:26840129

  5. What Is the Best NGS Enrichment Method for the Molecular Diagnosis of Monogenic Diabetes and Obesity?

    PubMed

    Philippe, Julien; Derhourhi, Mehdi; Durand, Emmanuelle; Vaillant, Emmanuel; Dechaume, Aurélie; Rabearivelo, Iandry; Dhennin, Véronique; Vaxillaire, Martine; De Graeve, Franck; Sand, Olivier; Froguel, Philippe; Bonnefond, Amélie

    2015-01-01

    Molecular diagnosis of monogenic diabetes and obesity is of paramount importance for both the patient and society, as it can result in personalized medicine associated with a better life and it eventually saves health care spending. Genetic clinical laboratories are currently switching from Sanger sequencing to next-generation sequencing (NGS) approaches but choosing the optimal protocols is not easy. Here, we compared the sequencing coverage of 43 genes involved in monogenic forms of diabetes and obesity, and variant detection rates, resulting from four enrichment methods based on the sonication of DNA (Agilent SureSelect, RainDance technologies), or using enzymes for DNA fragmentation (Illumina Nextera, Agilent HaloPlex). We analyzed coding exons and untranslated regions of the 43 genes involved in monogenic diabetes and obesity. We found that none of the methods achieves yet full sequencing of the gene targets. Nonetheless, the RainDance, SureSelect and HaloPlex enrichment methods led to the best sequencing coverage of the targets; while the Nextera method resulted in the poorest sequencing coverage. Although the sequencing coverage was high, we unexpectedly found that the HaloPlex method missed 20% of variants detected by the three other methods and Nextera missed 10%. The question of which NGS technique for genetic diagnosis yields the highest diagnosis rate is frequently discussed in the literature and the response is still unclear. Here, we showed that the RainDance enrichment method as well as SureSelect, which are both based on the sonication of DNA, resulted in a good sequencing quality and variant detection, while the use of enzymes to fragment DNA (HaloPlex or Nextera) might not be the best strategy to get an accurate sequencing. PMID:26599467

  6. Expanding genotype/phenotype of neuromuscular diseases by comprehensive target capture/NGS

    PubMed Central

    Tian, Xia; Liang, Wen-Chen; Feng, Yanming; Wang, Jing; Zhang, Victor Wei; Chou, Chih-Hung; Huang, Hsien-Da; Lam, Ching Wan; Hsu, Ya-Yun; Lin, Thy-Sheng; Chen, Wan-Tzu

    2015-01-01

    Objective: To establish and evaluate the effectiveness of a comprehensive next-generation sequencing (NGS) approach to simultaneously analyze all genes known to be responsible for the most clinically and genetically heterogeneous neuromuscular diseases (NMDs) involving spinal motoneurons, neuromuscular junctions, nerves, and muscles. Methods: All coding exons and at least 20 bp of flanking intronic sequences of 236 genes causing NMDs were enriched by using SeqCap EZ solution-based capture and enrichment method followed by massively parallel sequencing on Illumina HiSeq2000. Results: The target gene capture/deep sequencing provides an average coverage of ∼1,000× per nucleotide. Thirty-five unrelated NMD families (38 patients) with clinical and/or muscle pathologic diagnoses but without identified causative genetic defects were analyzed. Deleterious mutations were found in 29 families (83%). Definitive causative mutations were identified in 21 families (60%) and likely diagnoses were established in 8 families (23%). Six families were left without diagnosis due to uncertainty in phenotype/genotype correlation and/or unidentified causative genes. Using this comprehensive panel, we not only identified mutations in expected genes but also expanded phenotype/genotype among different subcategories of NMDs. Conclusions: Target gene capture/deep sequencing approach can greatly improve the genetic diagnosis of NMDs. This study demonstrated the power of NGS in confirming and expanding clinical phenotypes/genotypes of the extremely heterogeneous NMDs. Confirmed molecular diagnoses of NMDs can assist in genetic counseling and carrier detection as well as guide therapeutic options for treatable disorders. PMID:27066551

  7. HapTree: a novel Bayesian framework for single individual polyplotyping using NGS data.

    PubMed

    Berger, Emily; Yorukoglu, Deniz; Peng, Jian; Berger, Bonnie

    2014-03-01

    As the more recent next-generation sequencing (NGS) technologies provide longer read sequences, the use of sequencing datasets for complete haplotype phasing is fast becoming a reality, allowing haplotype reconstruction of a single sequenced genome. Nearly all previous haplotype reconstruction studies have focused on diploid genomes and are rarely scalable to genomes with higher ploidy. Yet computational investigations into polyploid genomes carry great importance, impacting plant, yeast and fish genomics, as well as the studies of the evolution of modern-day eukaryotes and (epi)genetic interactions between copies of genes. In this paper, we describe a novel maximum-likelihood estimation framework, HapTree, for polyploid haplotype assembly of an individual genome using NGS read datasets. We evaluate the performance of HapTree on simulated polyploid sequencing read data modeled after Illumina sequencing technologies. For triploid and higher ploidy genomes, we demonstrate that HapTree substantially improves haplotype assembly accuracy and efficiency over the state-of-the-art; moreover, HapTree is the first scalable polyplotyping method for higher ploidy. As a proof of concept, we also test our method on real sequencing data from NA12878 (1000 Genomes Project) and evaluate the quality of assembled haplotypes with respect to trio-based diplotype annotation as the ground truth. The results indicate that HapTree significantly improves the switch accuracy within phased haplotype blocks as compared to existing haplotype assembly methods, while producing comparable minimum error correction (MEC) values. A summary of this paper appears in the proceedings of the RECOMB 2014 conference, April 2-5. PMID:24675685

  8. DEVELOPMENT OF ANALYTICAL METHODS FOR DETERMINING SUPPRESSOR CONCENTRATION IN THE MCU NEXT GENERATION SOLVENT (NGS)

    SciTech Connect

    Taylor-Pashow, K.; Fondeur, F.; White, T.; Diprete, D.; Milliken, C.

    2013-07-31

    Savannah River National Laboratory (SRNL) was tasked with identifying and developing at least one, but preferably two methods for quantifying the suppressor in the Next Generation Solvent (NGS) system. The suppressor is a guanidine derivative, N,N',N"-tris(3,7-dimethyloctyl)guanidine (TiDG). A list of 10 possible methods was generated, and screening experiments were performed for 8 of the 10 methods. After completion of the screening experiments, the non-aqueous acid-base titration was determined to be the most promising, and was selected for further development as the primary method. {sup 1}H NMR also showed promising results from the screening experiments, and this method was selected for further development as the secondary method. Other methods, including {sup 36}Cl radiocounting and ion chromatography, also showed promise; however, due to the similarity to the primary method (titration) and the inability to differentiate between TiDG and TOA (tri-n-ocytlamine) in the blended solvent, {sup 1}H NMR was selected over these methods. Analysis of radioactive samples obtained from real waste ESS (extraction, scrub, strip) testing using the titration method showed good results. Based on these results, the titration method was selected as the method of choice for TiDG measurement. {sup 1}H NMR has been selected as the secondary (back-up) method, and additional work is planned to further develop this method and to verify the method using radioactive samples. Procedures for analyzing radioactive samples of both pure NGS and blended solvent were developed and issued for the both methods.

  9. Cancer modelling in the NGS era - Part I: Emerging technology and initial modelling.

    PubMed

    Rovigatti, Ugo

    2015-11-01

    It is today indisputable that great progresses have been made in our molecular understanding of cancer cells, but an effective implementation of such knowledge into dramatic cancer-cures is still belated and yet desperately needed. This review gives a snapshot at where we stand today in this search for cancer understanding and definitive treatments, how far we have progressed and what are the major obstacles we will have to overcome both technologically and for disease modelling. In the first part, promising 3rd/4th Generation Sequencing Technologies will be summarized (particularly IonTorrent and OxfordNanopore technologies). Cancer modelling will be then reviewed from its origin in XIX Century Germany to today's NGS applications for cancer understanding and therapeutic interventions. Developments after Molecular Biology revolution (1953) are discussed as successions of three phases. The first, PH1, labelled "Clonal Outgrowth" (from 1960s to mid 1980s) was characterized by discoveries in cytogenetics (Nowell, Rowley) and viral oncology (Dulbecco, Bishop, Varmus), which demonstrated clonality. Treatments were consequently dominated by a "cytotoxic eradication" strategy with chemotherapeutic agents. In PH2, (from the mid 1980s to our days) the description of cancer as "Gene Networks" led to targeted-gene-therapies (TGTs). TGTs are the focus of Section 3: in view of their apparent failing (Ephemeral Therapies), alternative strategies will be discussed in review part II (particularly cancer immunotherapy, CIT). Additional Pitfalls impinge on the concepts of tumour heterogeneity (inter/intra; ITH). The described pitfalls set the basis for a new phase, PH3, which is called "NGS Era" and will be also discussed with ten emerging cancer models in the Review 2nd part. PMID:26427785

  10. e-DNA meta-barcoding: from NGS raw data to taxonomic profiling.

    PubMed

    Bruno, Fosso; Marinella, Marzano; Santamaria, Monica

    2015-01-01

    In recent years, thanks to the essential support provided by the Next-Generation Sequencing (NGS) technologies, Metagenomics is enabling the direct access to the taxonomic and functional composition of mixed microbial communities living in any environmental niche, without the prerequisite to isolate or culture the single organisms. This approach has already been successfully applied for the analysis of many habitats, such as water or soil natural environments, also characterized by extreme physical and chemical conditions, food supply chains, and animal organisms, including humans. A shotgun sequencing approach can lead to investigate both organisms and genes diversity. Anyway, if the purpose is limited to explore the taxonomic complexity, an amplicon-based approach, based on PCR-targeted sequencing of selected genetic species markers, commonly named "meta-barcodes", is desirable. Among the genomic regions most widely used for the discrimination of bacterial organisms, in some cases up to the species level, some hypervariable domains of the gene coding for the 16S rRNA occupy a prominent place. The amplification of a certain meta-barcode from a microbial community through the use of PCR primers able to work in the entire considered taxonomic group is the first task after the extraction of the total DNA. Generally, this step is followed by the high-throughput sequencing of the resulting amplicons libraries by means of a selected NGS platform. Finally, the interpretation of the huge amount of produced data requires appropriate bioinformatics tools and know-how in addition to efficient computational resources. Here a computational methodology suitable for the taxonomic characterization of 454 meta-barcode sequences is described in detail. In particular, a dataset covering the V1-V3 region belonging to the bacterial 16S rRNA coding gene and produced in the Human Microbiome Project (HMP) from a palatine tonsils sample is analyzed. The proposed exercise includes the

  11. 45 CFR 1176.5 - Annual plan.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Annual plan. 1176.5 Section 1176.5 Public Welfare... ENDOWMENT FOR THE HUMANITIES PART-TIME CAREER EMPLOYMENT § 1176.5 Annual plan. (a) An agencywide plan for promoting part-time employment opportunities will be developed annually. This plan will establish...

  12. 45 CFR 1176.5 - Annual plan.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Annual plan. 1176.5 Section 1176.5 Public Welfare... ENDOWMENT FOR THE HUMANITIES PART-TIME CAREER EMPLOYMENT § 1176.5 Annual plan. (a) An agencywide plan for promoting part-time employment opportunities will be developed annually. This plan will establish...

  13. 45 CFR 1176.5 - Annual plan.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Annual plan. 1176.5 Section 1176.5 Public Welfare... ENDOWMENT FOR THE HUMANITIES PART-TIME CAREER EMPLOYMENT § 1176.5 Annual plan. (a) An agencywide plan for promoting part-time employment opportunities will be developed annually. This plan will establish...

  14. 45 CFR 1176.5 - Annual plan.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Annual plan. 1176.5 Section 1176.5 Public Welfare... ENDOWMENT FOR THE HUMANITIES PART-TIME CAREER EMPLOYMENT § 1176.5 Annual plan. (a) An agencywide plan for promoting part-time employment opportunities will be developed annually. This plan will establish...

  15. 45 CFR 1176.5 - Annual plan.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Annual plan. 1176.5 Section 1176.5 Public Welfare... ENDOWMENT FOR THE HUMANITIES PART-TIME CAREER EMPLOYMENT § 1176.5 Annual plan. (a) An agencywide plan for promoting part-time employment opportunities will be developed annually. This plan will establish...

  16. 45 CFR 671.14 - Annual review.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Annual review. 671.14 Section 671.14 Public... Designation of Banned Substances; Reclassification of Pollutants § 671.14 Annual review. The Director shall review the list of banned substances and designated pollutants at least annually, and may propose...

  17. 45 CFR 671.14 - Annual review.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Annual review. 671.14 Section 671.14 Public... Designation of Banned Substances; Reclassification of Pollutants § 671.14 Annual review. The Director shall review the list of banned substances and designated pollutants at least annually, and may propose...

  18. 42 CFR 419.50 - Annual review.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 3 2012-10-01 2012-10-01 false Annual review. 419.50 Section 419.50 Public Health....50 Annual review. (a) General rule. Not less often than annually, CMS reviews and updates groups... composed of an appropriate selection of representatives of providers to review (and advise CMS...

  19. 36 CFR 901.5 - Annual report.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Annual report. 901.5 Section 901.5 Parks, Forests, and Public Property PENNSYLVANIA AVENUE DEVELOPMENT CORPORATION BYLAWS OF THE CORPORATION § 901.5 Annual report. The Executive Director shall prepare annually a comprehensive and...

  20. 36 CFR 901.5 - Annual report.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Annual report. 901.5 Section 901.5 Parks, Forests, and Public Property PENNSYLVANIA AVENUE DEVELOPMENT CORPORATION BYLAWS OF THE CORPORATION § 901.5 Annual report. The Executive Director shall prepare annually a comprehensive and...

  1. 45 CFR 2400.2 - Annual competition.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Annual competition. 2400.2 Section 2400.2 Public... FELLOWSHIP PROGRAM REQUIREMENTS General § 2400.2 Annual competition. To achieve its principal purposes, the Foundation holds an annual national competition to select teachers in grades 7-12, college seniors,...

  2. 45 CFR 2400.2 - Annual competition.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Annual competition. 2400.2 Section 2400.2 Public... FELLOWSHIP PROGRAM REQUIREMENTS General § 2400.2 Annual competition. To achieve its principal purposes, the Foundation holds an annual national competition to select teachers in grades 7-12, college seniors,...

  3. 45 CFR 2400.2 - Annual competition.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Annual competition. 2400.2 Section 2400.2 Public... FELLOWSHIP PROGRAM REQUIREMENTS General § 2400.2 Annual competition. To achieve its principal purposes, the Foundation holds an annual national competition to select teachers in grades 7-12, college seniors,...

  4. 45 CFR 2400.2 - Annual competition.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Annual competition. 2400.2 Section 2400.2 Public... FELLOWSHIP PROGRAM REQUIREMENTS General § 2400.2 Annual competition. To achieve its principal purposes, the Foundation holds an annual national competition to select teachers in grades 7-12, college seniors,...

  5. 45 CFR 2400.2 - Annual competition.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Annual competition. 2400.2 Section 2400.2 Public... FELLOWSHIP PROGRAM REQUIREMENTS General § 2400.2 Annual competition. To achieve its principal purposes, the Foundation holds an annual national competition to select teachers in grades 7-12, college seniors,...

  6. 42 CFR 457.750 - Annual report.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 4 2012-10-01 2012-10-01 false Annual report. 457.750 Section 457.750 Public..., Reporting, and Evaluation § 457.750 Annual report. (a) Report required for each Federal fiscal year. A State... State's assessment of the operation of the State plan. (b) Contents of annual report. In the...

  7. 42 CFR 457.750 - Annual report.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 4 2013-10-01 2013-10-01 false Annual report. 457.750 Section 457.750 Public..., Reporting, and Evaluation § 457.750 Annual report. (a) Report required for each Federal fiscal year. A State... State's assessment of the operation of the State plan. (b) Contents of annual report. In the...

  8. 42 CFR 457.750 - Annual report.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 4 2014-10-01 2014-10-01 false Annual report. 457.750 Section 457.750 Public..., Reporting, and Evaluation § 457.750 Annual report. (a) Report required for each Federal fiscal year. A State... State's assessment of the operation of the State plan. (b) Contents of annual report. In the...

  9. Photometric calibration of NGS/POSS and ESO/SRC plates using the NOAO PDS measuring engine. II - Surface photometry

    NASA Technical Reports Server (NTRS)

    Cutri, Roc M.; Low, Frank J.; Guhathakurta, Puragra

    1993-01-01

    In this paper we present a method to calibrate surface photometry of faint sources measured from direct photographic plates, such as those of the NGS/POSS and ESO/SRC Sky Survey. This calibration procedure does not require scanning sensitometer spots on the plates, but instead uses measurements of the brightness profiles of many faint stars of known brightness to fit a linearized approximation to the characteristic curve. The approximation is valid for only low- to medium-density emulsions, so this technique is appropriate only for relatively faint emission. Comparison between measurements of representative extended sources on the NGS/POSS and CCD images indicates that surface photometry can be obtained from the Sky Survey plates accurate to 0.1-0.3 mag in the range mu(B) between 23 and 27 and mu(R) between 22 and 26 mag/sq arcsec.

  10. Implementation and on-sky results of an optimal wavefront controller for the MMT NGS adaptive optics system

    NASA Astrophysics Data System (ADS)

    Powell, Keith B.; Vaitheeswaran, Vidhya

    2010-07-01

    The MMT observatory has recently implemented and tested an optimal wavefront controller for the NGS adaptive optics system. Open loop atmospheric data collected at the telescope is used as the input to a MATLAB based analytical model. The model uses nonlinear constrained minimization to determine controller gains and optimize the system performance. The real-time controller performing the adaptive optics close loop operation is implemented on a dedicated high performance PC based quad core server. The controller algorithm is written in C and uses the GNU scientific library for linear algebra. Tests at the MMT confirmed the optimal controller significantly reduced the residual RMS wavefront compared with the previous controller. Significant reductions in image FWHM and increased peak intensities were obtained in J, H and K-bands. The optimal PID controller is now operating as the baseline wavefront controller for the MMT NGS-AO system.

  11. Effective Analysis of NGS Metagenomic Data with Ultra-Fast Clustering Algorithms (MICW - Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

    ScienceCinema

    Li, Weizhong [San Diego Supercomputer Center

    2013-01-22

    San Diego Supercomputer Center's Weizhong Li on "Effective Analysis of NGS Metagenomic Data with Ultra-fast Clustering Algorithms" at the Metagenomics Informatics Challenges Workshop held at the DOE JGI on October 12-13, 2011.

  12. Effective Analysis of NGS Metagenomic Data with Ultra-Fast Clustering Algorithms (MICW - Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

    SciTech Connect

    Li, Weizhong

    2011-10-12

    San Diego Supercomputer Center's Weizhong Li on "Effective Analysis of NGS Metagenomic Data with Ultra-fast Clustering Algorithms" at the Metagenomics Informatics Challenges Workshop held at the DOE JGI on October 12-13, 2011.

  13. Profiling of the dynamically alteredgene expression in peripheral nerve injury using NGS RNA sequencing technique.

    PubMed

    Han, Duanyang; Chen, Yixun; Kou, Yuhui; Weng, Jian; Chen, Bo; Yu, Youlai; Zhang, Peixun; Jiang, Baoguo

    2016-01-01

    Functional recovery of peripheral nerve injuries is of major demand in clinical practice worldwide. Although, to some extent, peripheral nervous system can spontaneously regenerate, post-injury recovery is often associated with poor functional outcome. The molecular mechanism controlling the peripheral nerve repair process is still majorly unclear. In this study, by utilizing the Next Generation Sequencing (NGS) RNA sequencing technique, we aim to profile the gene expression spectrum of the peripheral nerve repair. In total, we detected 2847 were differentially expressed at day 7 post crush nerve injury. The GO, Panther, IPA and GSEA analysis was performed to decipher the biological processes involving the differentially expressed genes. Collectively, our results highlighted the inflammatory response and related signaling pathway (NFkB and TNFa signaling) play key role in peripheral nerve repair regulation. Furthermore, Network analysis illustrated that the IL10, IL18, IFN-γ and PDCD1 were four key regulators with multiple participations in peripheral nerve repair and potentially exert influence to the repair process. The expression changes of IL10, IL18, IFN-γ, PDCD1 and TNFSF14 (LIGHT) were further validated by western blot analysis. Hopefully, the present study may provide useful platform to further reveal the molecular mechanism of peripheral nerve repair and discover promising treatment target to enhance peripheral nerve regeneration. PMID:27158375

  14. Profiling of the dynamically alteredgene expression in peripheral nerve injury using NGS RNA sequencing technique

    PubMed Central

    Han, Duanyang; Chen, Yixun; Kou, Yuhui; Weng, Jian; Chen, Bo; Yu, Youlai; Zhang, Peixun; Jiang, Baoguo

    2016-01-01

    Functional recovery of peripheral nerve injuries is of major demand in clinical practice worldwide. Although, to some extent, peripheral nervous system can spontaneously regenerate, post-injury recovery is often associated with poor functional outcome. The molecular mechanism controlling the peripheral nerve repair process is still majorly unclear. In this study, by utilizing the Next Generation Sequencing (NGS) RNA sequencing technique, we aim to profile the gene expression spectrum of the peripheral nerve repair. In total, we detected 2847 were differentially expressed at day 7 post crush nerve injury. The GO, Panther, IPA and GSEA analysis was performed to decipher the biological processes involving the differentially expressed genes. Collectively, our results highlighted the inflammatory response and related signaling pathway (NFkB and TNFa signaling) play key role in peripheral nerve repair regulation. Furthermore, Network analysis illustrated that the IL10, IL18, IFN-γ and PDCD1 were four key regulators with multiple participations in peripheral nerve repair and potentially exert influence to the repair process. The expression changes of IL10, IL18, IFN-γ, PDCD1 and TNFSF14 (LIGHT) were further validated by western blot analysis. Hopefully, the present study may provide useful platform to further reveal the molecular mechanism of peripheral nerve repair and discover promising treatment target to enhance peripheral nerve regeneration. PMID:27158375

  15. IgH-V(D)J NGS-MRD measurement pre- and early post-allotransplant defines very low- and very high-risk ALL patients

    PubMed Central

    Carlson, Chris; Langholz, Bryan; Wall, Donna A.; Schultz, Kirk R.; Bunin, Nancy; Kirsch, Ilan; Gastier-Foster, Julie M.; Borowitz, Michael; Desmarais, Cindy; Williamson, David; Kalos, Michael; Grupp, Stephan A.

    2015-01-01

    Positive detection of minimal residual disease (MRD) by multichannel flow cytometry (MFC) prior to hematopoietic cell transplantation (HCT) of patients with acute lymphoblastic leukemia (ALL) identifies patients at high risk for relapse, but many pre-HCT MFC-MRD negative patients also relapse, and the predictive power MFC-MRD early post-HCT is poor. To test whether the increased sensitivity of next-generation sequencing (NGS)–MRD better identifies pre- and post-HCT relapse risk, we performed immunoglobulin heavy chain (IgH) variable, diversity, and joining (V[D]J) DNA sequences J NGS-MRD on 56 patients with B-cell ALL enrolled in Children’s Oncology Group trial ASCT0431. NGS-MRD predicted relapse and survival more accurately than MFC-MRD (P < .0001), especially in the MRD negative cohort (relapse, 0% vs 16%; P = .02; 2-year overall survival, 96% vs 77%; P = .003). Post-HCT NGS-MRD detection was better at predicting relapse than MFC-MRD (P < .0001), especially early after HCT (day 30 MFC-MRD positive relapse rate, 35%; NGS-MRD positive relapse rate, 67%; P = .004). Any post-HCT NGS positivity resulted in an increase in relapse risk by multivariate analysis (hazard ratio, 7.7; P = .05). Absence of detectable IgH-V(D)J NGS-MRD pre-HCT defines good-risk patients potentially eligible for less intense treatment approaches. Post-HCT NGS-MRD is highly predictive of relapse and survival, suggesting a role for this technique in defining patients early who would be eligible for post-HCT interventions. The trial was registered at www.clinicaltrials.gov as #NCT00382109. PMID:25862561

  16. NGS Transcriptomes and Enzyme Inhibitors Unravel Complexity of Picrosides Biosynthesis in Picrorhiza kurroa Royle ex. Benth

    PubMed Central

    Shitiz, Kirti; Sharma, Neha; Pal, Tarun; Sood, Hemant; Chauhan, Rajinder S.

    2015-01-01

    Picrorhiza kurroa is an important medicinal herb valued for iridoid glycosides, Picroside-I (P-I) and Picroside-II (P-II), which have several pharmacological activities. Genetic interventions for developing a picroside production platform would require knowledge on biosynthetic pathway and key control points, which does not exist as of today. The current study reports that geranyl pyrophosphate (GPP) moiety is mainly contributed by the non-mevalonate (MEP) route, which is further modified to P-I and P-II through phenylpropanoid and iridoid pathways, in total consisting of 41 and 35 enzymatic steps, respectively. The role of the MEP pathway was ascertained through enzyme inhibitors fosmidomycin and mevinolin along with importance of other integrating pathways using glyphosate, aminooxy acetic acid (AOA) and actinomycin D, which overall resulted in 17%-92% inhibition of P-I accumulation. Retrieval of gene sequences for enzymatic steps from NGS transcriptomes and their expression analysis vis-à-vis picrosides content in different tissues/organs showed elevated transcripts for twenty genes, which were further shortlisted to seven key genes, ISPD, DXPS, ISPE, PMK, 2HFD, EPSPS and SK, on the basis of expression analysis between high versus low picrosides content strains of P. kurroa so as to eliminate tissue type/ developmental variations in picrosides contents. The higher expression of the majority of the MEP pathway genes (ISPD, DXPS and ISPE), coupled with higher inhibition of DXPR enzyme by fosmidomycin, suggested that the MEP route contributed to the biosynthesis of P-I in P. kurroa. The outcome of the study is expected to be useful in designing a suitable genetic intervention strategy towards enhanced production of picrosides. Possible key genes contributing to picroside biosynthesis have been identified with potential implications in molecular breeding and metabolic engineering of P. kurroa. PMID:26658062

  17. NGS Transcriptomes and Enzyme Inhibitors Unravel Complexity of Picrosides Biosynthesis in Picrorhiza kurroa Royle ex. Benth.

    PubMed

    Shitiz, Kirti; Sharma, Neha; Pal, Tarun; Sood, Hemant; Chauhan, Rajinder S

    2015-01-01

    Picrorhiza kurroa is an important medicinal herb valued for iridoid glycosides, Picroside-I (P-I) and Picroside-II (P-II), which have several pharmacological activities. Genetic interventions for developing a picroside production platform would require knowledge on biosynthetic pathway and key control points, which does not exist as of today. The current study reports that geranyl pyrophosphate (GPP) moiety is mainly contributed by the non-mevalonate (MEP) route, which is further modified to P-I and P-II through phenylpropanoid and iridoid pathways, in total consisting of 41 and 35 enzymatic steps, respectively. The role of the MEP pathway was ascertained through enzyme inhibitors fosmidomycin and mevinolin along with importance of other integrating pathways using glyphosate, aminooxy acetic acid (AOA) and actinomycin D, which overall resulted in 17%-92% inhibition of P-I accumulation. Retrieval of gene sequences for enzymatic steps from NGS transcriptomes and their expression analysis vis-à-vis picrosides content in different tissues/organs showed elevated transcripts for twenty genes, which were further shortlisted to seven key genes, ISPD, DXPS, ISPE, PMK, 2HFD, EPSPS and SK, on the basis of expression analysis between high versus low picrosides content strains of P. kurroa so as to eliminate tissue type/ developmental variations in picrosides contents. The higher expression of the majority of the MEP pathway genes (ISPD, DXPS and ISPE), coupled with higher inhibition of DXPR enzyme by fosmidomycin, suggested that the MEP route contributed to the biosynthesis of P-I in P. kurroa. The outcome of the study is expected to be useful in designing a suitable genetic intervention strategy towards enhanced production of picrosides. Possible key genes contributing to picroside biosynthesis have been identified with potential implications in molecular breeding and metabolic engineering of P. kurroa. PMID:26658062

  18. Next Generation Solvent (NGS): Development for Caustic-Side Solvent Extraction of Cesium

    SciTech Connect

    Moyer, Bruce A.; Birdwell, Jr, Joseph F.; Bonnesen, Peter V.; Bruffey, Stephanie H.; Delmau, Laetitia Helene; Duncan, Nathan C.; Ensor, Dale; Hill, Talon G.; Lee, Denise L.; Rajbanshi, Arbin; Roach, Benjamin D.; Szczygiel, Patricia L.; Frederick V. Sloop, Jr.; Stoner, Erica L.; Williams, Neil J.

    2014-03-01

    This report summarizes the FY 2010 and 2011 accomplishments at Oak Ridge National Laboratory (ORNL) in developing the Next Generation Caustic-Side Solvent Extraction (NG-CSSX) process, referred to commonly as the Next Generation Solvent (NGS), under funding from the U.S. Department of Energy, Office of Environmental Management (DOE-EM), Office of Technology Innovation and Development. The primary product of this effort is a process solvent and preliminary flowsheet capable of meeting a target decontamination factor (DF) of 40,000 for worst-case Savannah River Site (SRS) waste with a concentration factor of 15 or higher in the 18-stage equipment configuration of the SRS Modular Caustic-Side Solvent Extraction Unit (MCU). In addition, the NG-CSSX process may be readily adapted for use in the SRS Salt Waste Processing Facility (SWPF) or in supplemental tank-waste treatment at Hanford upon appropriate solvent or flowsheet modifications. Efforts in FY 2010 focused on developing a solvent composition and process flowsheet for MCU implementation. In FY 2011 accomplishments at ORNL involved a wide array of chemical-development activities and testing up through single-stage hydraulic and mass-transfer tests in 5-cm centrifugal contactors. Under subcontract from ORNL, Argonne National Laboratory (ANL) designed a preliminary flowsheet using ORNL cesium distribution data, and Tennessee Technological University confirmed a chemical model for cesium distribution ratios (DCs) as a function of feed composition. Interlaboratory efforts were coordinated with complementary engineering tests carried out (and reported separately) by personnel at Savannah River National Laboratory (SRNL) and Savannah River Remediation (SRR) with helpful advice by Parsons Engineering and General Atomics on aspects of possible SWPF implementation.

  19. MicNeSs: genotyping microsatellite loci from a collection of (NGS) reads.

    PubMed

    Suez, Marie; Behdenna, Abdelkader; Brouillet, Sophie; Graça, Paula; Higuet, Dominique; Achaz, Guillaume

    2016-03-01

    Microsatellites are widely used in population genetics to uncover recent evolutionary events. They are typically genotyped using capillary sequencer, which capacity is usually limited to 9, at most 12 loci for each run, and which analysis is a tedious task that is performed by hand. With the rise of next-generation sequencing (NGS), a much larger number of loci and individuals are available from sequencing: for example, on a single run of a GS Junior, 28 loci from 96 individuals are sequenced with a 30X cover. We have developed an algorithm to automatically and efficiently genotype microsatellites from a collection of reads sorted by individual (e.g. specific PCR amplifications of a locus or a collection of reads that encompass a locus of interest). As the sequencing and the PCR amplification introduce artefactual insertions or deletions, the set of reads from a single microsatellite allele shows several length variants. The algorithm infers, without alignment, the true unknown allele(s) of each individual from the observed distributions of microsatellites length of all individuals. MicNeSs, a python implementation of the algorithm, can be used to genotype any microsatellite locus from any organism and has been tested on 454 pyrosequencing data of several loci from fruit flies (a model species) and red deers (a nonmodel species). Without any parallelization, it automatically genotypes 22 loci from 441 individuals in 11 hours on a standard computer. The comparison of MicNeSs inferences to the standard method shows an excellent agreement, with some differences illustrating the pros and cons of both methods. PMID:26400716

  20. 45 CFR 1801.11 - Annual nomination.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Annual nomination. 1801.11 Section 1801.11 Public Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Nominations § 1801.11 Annual nomination. (a) Except as provided in §§...

  1. 45 CFR 1801.11 - Annual nomination.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Annual nomination. 1801.11 Section 1801.11 Public Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Nominations § 1801.11 Annual nomination. (a) Except as provided in §§...

  2. 45 CFR 1801.11 - Annual nomination.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Annual nomination. 1801.11 Section 1801.11 Public Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Nominations § 1801.11 Annual nomination. (a) Except as provided in §§...

  3. 45 CFR 1801.11 - Annual nomination.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Annual nomination. 1801.11 Section 1801.11 Public Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Nominations § 1801.11 Annual nomination. (a) Except as provided in §§...

  4. 45 CFR 1801.11 - Annual nomination.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Annual nomination. 1801.11 Section 1801.11 Public Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Nominations § 1801.11 Annual nomination. (a) Except as provided in §§...

  5. 45 CFR 1620.5 - Annual review.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Annual review. 1620.5 Section 1620.5 Public Welfare Regulations Relating to Public Welfare (Continued) LEGAL SERVICES CORPORATION PRIORITIES IN USE OF RESOURCES § 1620.5 Annual review. (a) Priorities shall be set periodically and shall be reviewed by...

  6. 42 CFR 51.8 - Annual reports.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Annual reports. 51.8 Section 51.8 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS REQUIREMENTS APPLICABLE TO THE PROTECTION AND ADVOCACY FOR INDIVIDUALS WITH MENTAL ILLNESS PROGRAM Basic Requirements § 51.8 Annual...

  7. Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (80th, Chicago, Illinois, July 30-August 2, 1997): Public Relations.

    ERIC Educational Resources Information Center

    Association for Education in Journalism and Mass Communication.

    The Public Relations section of the Proceedings contains the following 13 papers: "Pluralistic Ignorance and Educators in Public Relations: Underestimating Professionalism of Our Educator Peers and of Practitioners in the Field" (Lynne M. Sallot; Glen T. Cameron; Ruth Ann Weaver-Lariscy); "Critical Conflict Issues in Public Relations Agency-Client…

  8. Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (86th, Kansas City, Missouri, July 30-August 2, 2003). Public Relations Division.

    ERIC Educational Resources Information Center

    2003

    The Public Relations Division of the proceedings contains the following 20 papers: "Communicating for Technical Change: Business-to-Business Communication with Small Manufacturing Firms" (Danielle Pontiff); "Integrating Editorial Presentation and Public Relations Publications: New Frontiers for Convergence and Collaborative Learning" (Jennifer…

  9. Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (81st, Baltimore, Maryland, August 5-8, 1988). Public Relations.

    ERIC Educational Resources Information Center

    Association for Education in Journalism and Mass Communication.

    The Public Relations section of the Proceedings contains the following 15 papers: "The World Wide Web as a Public Relations Medium: The Use of Research, Planning, and Evaluation by Web Site Decision-Makers" (Candace White and Niranjan Raman); "Enlightened Self Interest--An Ethical Baseline for Teaching Corporate Public Relations" (Patricia T.…

  10. Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (85th, Miami, Florida, August 5-8, 2002). Public Relations Division.

    ERIC Educational Resources Information Center

    2002

    The Public Relations Division of the proceedings contains the following 15 papers: "Virtual Issues in Traditional Texts: How Introductory Public Relations Textbooks Address Internet Technology Issues" (Lois A. Boynton and Cassandra Imfeld Gajkowski); "Crisis Public Relations: A Study of Leadership, Culture, Demand and Delivery" (Terence (Terry)…

  11. Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (74th, Boston, Massachusetts, August 7-10, 1991). Part XV: Public Relations.

    ERIC Educational Resources Information Center

    Association for Education in Journalism and Mass Communication.

    The Public Relations section of the proceedings contains the following 14 papers: "Telling the Truth in Public Relations: An Application of the Narrative Paradigm" (Stephen P. Banks); "Teaching Fund Raising in Public Relations Programs: A National Study of the Attitudes and Behaviors of Educators" (Kathleen S. Kelly); "The Effect of World View on…

  12. The Community Care Program Makes a Difference. Annual Report to the Governor and the Illinois General Assembly on Public Act 81-202, Fiscal Year 1986.

    ERIC Educational Resources Information Center

    Illinois State Dept. on Aging, Springfield.

    This document contains an annual report on Illinois' Community Care Program (CCP), a program which provides in-home and other support services to help older persons to remain living at home and avoid premature institutionalization. Three levels of care provided by the CCP are described: (1) chore housekeeping, consisting of basic household tasks…

  13. Semi-Annual Report to Congress: April 1, 1981-September 30, 1981. No. 3. (Submitted Pursuant to Public Law 95-452).

    ERIC Educational Resources Information Center

    Office of Inspector General (ED), Washington, DC.

    The Office of Inspector General (OIG), mandated to provide audit, investigation, fraud detection and prevention, and some security services to the U.S. Department of Education, presents its third semi-annual report in this document. OIG audit activities are recounted in the first section, which details audit accomplishments and highlights audits…

  14. UNICEF Annual Report 1983.

    ERIC Educational Resources Information Center

    United Nations Children's Fund, New York, NY.

    In introducing this annual report, the executive director of UNICEF delineates the four techniques for primary health care and basic services reported in the publication "State of the World's Children, 1982-1983." The ensuing review of UNICEF's activities illustrates highlights of the year's program cooperation, including trends and key events, by…

  15. Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (82nd, New Orleans, Louisiana, August 3-8, 1999). Public Relations.

    ERIC Educational Resources Information Center

    Association for Education in Journalism and Mass Communication.

    The Public Relations section of the Proceedings contains the following 8 papers: "The Cultural Competence Spiral: An Assessment and Profile of U.S. Public Relations Practitioners' Preparation for International Assignments" (Alan R. Freitag); "Trust, Communication, and Quality Relationships in the Workplace: Comparing Views of CEOs and Top…

  16. 28 CFR 16.208 - Annual report.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Annual report. 16.208 Section 16.208 Judicial Administration DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Public Observation of Parole Commission Meetings § 16.208 Annual report. The Commission shall report annually...

  17. 28 CFR 16.208 - Annual report.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 1 2014-07-01 2014-07-01 false Annual report. 16.208 Section 16.208 Judicial Administration DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Public Observation of Parole Commission Meetings § 16.208 Annual report. The Commission shall report annually...

  18. 28 CFR 16.208 - Annual report.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 1 2012-07-01 2012-07-01 false Annual report. 16.208 Section 16.208 Judicial Administration DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Public Observation of Parole Commission Meetings § 16.208 Annual report. The Commission shall report annually...

  19. 28 CFR 16.208 - Annual report.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 1 2013-07-01 2013-07-01 false Annual report. 16.208 Section 16.208 Judicial Administration DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Public Observation of Parole Commission Meetings § 16.208 Annual report. The Commission shall report annually...

  20. 28 CFR 16.208 - Annual report.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Annual report. 16.208 Section 16.208 Judicial Administration DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Public Observation of Parole Commission Meetings § 16.208 Annual report. The Commission shall report annually...

  1. 22 CFR 62.15 - Annual reports.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Annual reports. 62.15 Section 62.15 Foreign Relations DEPARTMENT OF STATE PUBLIC DIPLOMACY AND EXCHANGES EXCHANGE VISITOR PROGRAM General Provisions § 62.15 Annual reports. Sponsors shall submit an annual report to the Department of State....

  2. 22 CFR 62.15 - Annual reports.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Annual reports. 62.15 Section 62.15 Foreign Relations DEPARTMENT OF STATE PUBLIC DIPLOMACY AND EXCHANGES EXCHANGE VISITOR PROGRAM General Provisions § 62.15 Annual reports. Sponsors shall submit an annual report to the Department of State....

  3. 22 CFR 62.15 - Annual reports.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Annual reports. 62.15 Section 62.15 Foreign Relations DEPARTMENT OF STATE PUBLIC DIPLOMACY AND EXCHANGES EXCHANGE VISITOR PROGRAM General Provisions § 62.15 Annual reports. Sponsors shall submit an annual report to the Department of State....

  4. Comparative study of aCGH and Next Generation Sequencing (NGS) for chromosomal microdeletion and microduplication screening

    PubMed Central

    Russo, Claudio Dello; Di Giacomo, Gianluca; Cignini, Pietro; Padula, Francesco; Mangiafico, Lucia; Mesoraca, Alvaro; D’Emidio, Laura; McCluskey, Megan R.; Paganelli, Arianna; Giorlandino, Claudio

    2014-01-01

    Background prenatal genetic diagnosis of rare disorders is undergoing in recent years a significant enhancement through the application of methods of massive parallel sequencing. Despite the quantity and quality of the data produced, just few analytical tools and software have been developed in order to identify structural and numerical chromosomal anomalies through NGS, mostly not compatible with benchtop NGS platform and routine clinical diagnosis. Methods we developed technical, bioinformatic, interpretive and validation pipelines for Next Generation Sequencing to identify SNPs, indels, aneuploidies, and CNVs (Copy Number Variations). Results we show a new targeted resequencing approach applied to prenatal diagnosis. For sample processing we used an enrichment method for 4,813 genes library preparation; after sequencing our bioinformatic pipelines allowed both SNPs analysis for approximately thirty diseases or diseases family involved in fetus development and numerical chromosomal anomalies screening. Conclusions results obtained are compatible with those obtained through the gold standard technique, aCGH array, moreover allowing identification of genes involved in chromosome deletions or duplications and exclusion of point mutation on allele not affected by chromosome aberrations. PMID:26266003

  5. Using GC-FID to Quantify the Removal of 4-sec-Butylphenol from NGS Solvent by NaOH

    SciTech Connect

    Sloop, Jr., Frederick V.; Moyer, Bruce A.

    2014-12-01

    A caustic wash of the solvent used in the Next-Generation Caustic-Side Solvent Extraction (NG-CSSX) process was found to remove the modifier breakdown product 4-sec-butylphenol (SBP) with varying efficiency depending on the aqueous NaOH concentration. Recent efforts at ORNL have aimed at characterizing the flowsheet chemistry and reducing the technical uncertainties of the NG-CSSX process. One technical uncertainty has been the efficacy of caustic washing of the solvent for the removal of lipophilic anions, in particular, the efficient removal of SBP, an important degradation product of the solvent modifier, Cs-7SB. In order to make this determination, it was necessary to develop a sensitive and reliable analytical technique for the detection and quantitation of SBP. This report recounts the development of a GC-FID-based (Gas Chromatography Flame Ionization Detection) technique for analyzing SBP and the utilization of the technique to subsequently confirm the ability of the caustic wash to efficiently remove SBP from the Next Generation Solvent (NGS) used in NG-CSSX. In particular, the developed technique was used to monitor the amount of SBP removed from a simple solvent and the full NGS by contact with sodium hydroxide wash solutions over a range of concentrations. The results show that caustic washing removes SBP with effectively the same efficiency as it did in the original Caustic-Side Solvent Extraction (CSSX) process.

  6. NGS-QCbox and Raspberry for Parallel, Automated and Rapid Quality Control Analysis of Large-Scale Next Generation Sequencing (Illumina) Data

    PubMed Central

    Katta, Mohan A. V. S. K.; Khan, Aamir W.; Doddamani, Dadakhalandar; Thudi, Mahendar; Varshney, Rajeev K.

    2015-01-01

    Rapid popularity and adaptation of next generation sequencing (NGS) approaches have generated huge volumes of data. High throughput platforms like Illumina HiSeq produce terabytes of raw data that requires quick processing. Quality control of the data is an important component prior to the downstream analyses. To address these issues, we have developed a quality control pipeline, NGS-QCbox that scales up to process hundreds or thousands of samples. Raspberry is an in-house tool, developed in C language utilizing HTSlib (v1.2.1) (http://htslib.org), for computing read/base level statistics. It can be used as stand-alone application and can process both compressed and uncompressed FASTQ format files. NGS-QCbox integrates Raspberry with other open-source tools for alignment (Bowtie2), SNP calling (SAMtools) and other utilities (bedtools) towards analyzing raw NGS data at higher efficiency and in high-throughput manner. The pipeline implements batch processing of jobs using Bpipe (https://github.com/ssadedin/bpipe) in parallel and internally, a fine grained task parallelization utilizing OpenMP. It reports read and base statistics along with genome coverage and variants in a user friendly format. The pipeline developed presents a simple menu driven interface and can be used in either quick or complete mode. In addition, the pipeline in quick mode outperforms in speed against other similar existing QC pipeline/tools. The NGS-QCbox pipeline, Raspberry tool and associated scripts are made available at the URL https://github.com/CEG-ICRISAT/NGS-QCbox and https://github.com/CEG-ICRISAT/Raspberry for rapid quality control analysis of large-scale next generation sequencing (Illumina) data. PMID:26460497

  7. NGS-QCbox and Raspberry for Parallel, Automated and Rapid Quality Control Analysis of Large-Scale Next Generation Sequencing (Illumina) Data.

    PubMed

    Katta, Mohan A V S K; Khan, Aamir W; Doddamani, Dadakhalandar; Thudi, Mahendar; Varshney, Rajeev K

    2015-01-01

    Rapid popularity and adaptation of next generation sequencing (NGS) approaches have generated huge volumes of data. High throughput platforms like Illumina HiSeq produce terabytes of raw data that requires quick processing. Quality control of the data is an important component prior to the downstream analyses. To address these issues, we have developed a quality control pipeline, NGS-QCbox that scales up to process hundreds or thousands of samples. Raspberry is an in-house tool, developed in C language utilizing HTSlib (v1.2.1) (http://htslib.org), for computing read/base level statistics. It can be used as stand-alone application and can process both compressed and uncompressed FASTQ format files. NGS-QCbox integrates Raspberry with other open-source tools for alignment (Bowtie2), SNP calling (SAMtools) and other utilities (bedtools) towards analyzing raw NGS data at higher efficiency and in high-throughput manner. The pipeline implements batch processing of jobs using Bpipe (https://github.com/ssadedin/bpipe) in parallel and internally, a fine grained task parallelization utilizing OpenMP. It reports read and base statistics along with genome coverage and variants in a user friendly format. The pipeline developed presents a simple menu driven interface and can be used in either quick or complete mode. In addition, the pipeline in quick mode outperforms in speed against other similar existing QC pipeline/tools. The NGS-QCbox pipeline, Raspberry tool and associated scripts are made available at the URL https://github.com/CEG-ICRISAT/NGS-QCbox and https://github.com/CEG-ICRISAT/Raspberry for rapid quality control analysis of large-scale next generation sequencing (Illumina) data. PMID:26460497

  8. Scheduled Salaries for Professional Personnel in Public Schools: 1994-95. Part 1 of National Survey of Salaries and Wages in Public Schools. 22nd Annual Edition. ERS Report.

    ERIC Educational Resources Information Center

    Educational Research Service, Arlington, VA.

    Much of the school-budget dollar goes to salaries and wages for employees. This document is the first part of a three-part report of the Educational Research Service (ERS) National Survey of Salaries and Wages in Public Schools. This volume reports data on salaries scheduled for 22 selected professional positions in 1,019 public school systems for…

  9. 42 CFR 1007.17 - Annual report.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Annual report. 1007.17 Section 1007.17 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OIG AUTHORITIES STATE MEDICAID FRAUD CONTROL UNITS § 1007.17 Annual report. At least 60 days prior to the expiration...

  10. 42 CFR 419.50 - Annual review.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 3 2010-10-01 2010-10-01 false Annual review. 419.50 Section 419.50 Public Health... review. (a) General rule. Not less often than annually, CMS reviews and updates groups, relative payment... selection of representatives of providers to review (and advise CMS concerning) the clinical integrity...

  11. 42 CFR 456.712 - Annual report.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 4 2013-10-01 2013-10-01 false Annual report. 456.712 Section 456.712 Public...) MEDICAL ASSISTANCE PROGRAMS UTILIZATION CONTROL Drug Use Review (DUR) Program and Electronic Claims Management System for Outpatient Drug Claims § 456.712 Annual report. (a) DUR Board report. The State...

  12. 42 CFR 456.712 - Annual report.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 4 2012-10-01 2012-10-01 false Annual report. 456.712 Section 456.712 Public...) MEDICAL ASSISTANCE PROGRAMS UTILIZATION CONTROL Drug Use Review (DUR) Program and Electronic Claims Management System for Outpatient Drug Claims § 456.712 Annual report. (a) DUR Board report. The State...

  13. 42 CFR 456.712 - Annual report.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 4 2014-10-01 2014-10-01 false Annual report. 456.712 Section 456.712 Public...) MEDICAL ASSISTANCE PROGRAMS UTILIZATION CONTROL Drug Use Review (DUR) Program and Electronic Claims Management System for Outpatient Drug Claims § 456.712 Annual report. (a) DUR Board report. The State...

  14. 42 CFR 51.8 - Annual reports.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Annual reports. 51.8 Section 51.8 Public Health... PROTECTION AND ADVOCACY FOR INDIVIDUALS WITH MENTAL ILLNESS PROGRAM Basic Requirements § 51.8 Annual reports... Office of Management and Budget....

  15. 42 CFR 51.8 - Annual reports.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Annual reports. 51.8 Section 51.8 Public Health... PROTECTION AND ADVOCACY FOR INDIVIDUALS WITH MENTAL ILLNESS PROGRAM Basic Requirements § 51.8 Annual reports... Office of Management and Budget....

  16. 42 CFR 51.8 - Annual reports.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Annual reports. 51.8 Section 51.8 Public Health... PROTECTION AND ADVOCACY FOR INDIVIDUALS WITH MENTAL ILLNESS PROGRAM Basic Requirements § 51.8 Annual reports... Office of Management and Budget....

  17. 42 CFR 51.8 - Annual reports.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Annual reports. 51.8 Section 51.8 Public Health... PROTECTION AND ADVOCACY FOR INDIVIDUALS WITH MENTAL ILLNESS PROGRAM Basic Requirements § 51.8 Annual reports... Date Note: At 62 FR 53564, Oct. 15, 1997, § 51.8 was added. This section contains...

  18. Administration of the Radiation Control for Health and Safety Act of 1968, Public Law 90-602, April 1, 1985 (1984 annual report). Report for 1 January-31 December 1984

    SciTech Connect

    Not Available

    1985-04-01

    The Secretary of Health and Human Services is required by Subpart 3, Part F of Title III of the Public Health Service Act; 42 USC 263b et seq. (Public Law 90-602) to submit an annual report to the President for transmittal to the Congress on or before April 1 on the administration of the Radiation Control for Health and Safety Act. The detailed information required to be included in the report is outlined in Section 360D of the Public Health Service Act. The Food and Drug Administration, through its Center for Devices and Radiological Health, is responsible for the day-to-day administration of the Act. The report covers the detailed operation of the Agency in carrying out that responsibility for calendar year 1984. Manufacturers of electronic products are required by 21 CFR 1002.20 to report accidental radiation occurrences to the CDRH, FDA. During the calendar year 1984, the Radiation Incidents Registry received 11 reports alleging injury involving 235 persons.

  19. Social Phenomena Leading to College Founding in Three States: The First Public Two-Year Colleges. ASHE 1986 Annual Meeting Paper.

    ERIC Educational Resources Information Center

    Ratcliff, James L.

    The reciprocal relationship of college founding and social factors in the external environment was studied in connection with the first public junior college in three states. The three cases were: Saginaw Junior College (reportedly established in 1895 in Michigan); McCook Junior College (1926, Nebraska); and Springfield Junior College (1917,…

  20. Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (84th, Washington, DC, August 5-8, 2001). Public Relations Division.

    ERIC Educational Resources Information Center

    Association for Education in Journalism and Mass Communication.

    The Public Relations section of the proceedings contains the following 11 selected papers: "If We Build It, Will They Come? Testing the Theory of Planned Behavior as a Predictive Model for Use in Determining How Career Counseling Centers Can Better Promote Their Facilities and Services" (Carolyn Ringer Lepre); "Student Preferences for University…

  1. Third Annual Desegregation Status Report for Public Postsecondary Education Institutions in the State of Maryland. Volume IV: Employees and Board Members.

    ERIC Educational Resources Information Center

    Maryland State Board for Higher Education, Annapolis.

    The data in this volume were collected in accordance with the 1974 Maryland plan for the desegregation of public post secondary education institutions. Presented are employee related data for Maryland colleges, universities and state agencies, and data on members of boards associated with higher education. The statistics for institutions include…

  2. Fiscal Year 1979 Maryland Amended Annual Program Plan Under Part B of the Education of the Handicapped Act as Amended by Public Law 94-142.

    ERIC Educational Resources Information Center

    Maryland State Dept. of Education, Baltimore. Office of Special Education.

    The document contains Maryland's 1979 program plan for the education of handicapped children as mandated by P.L. 94-142 (Education for All Handicapped Children Act). Seventeen chapters address the following: public notice and opportunity for comment; right to education policy statement; full educational goal and timelines; policy on priorities;…

  3. Assessing the Effectiveness of Public Research Universities: Using NSF/NCES Data and Data Envelopment Analysis Technique. AIR 2000 Annual Forum Paper.

    ERIC Educational Resources Information Center

    Zheng, Henry Y.; Stewart, Alice A.

    This study explores data envelopment analysis (DEA) as a tool for assessing and benchmarking the performance of public research universities. Using of national databases such as those conducted by the National Science Foundation and the National Center for Education Statistics, DEA analysis was conducted of the research and instructional outcomes…

  4. Discrepancies between Abstracts Presented at International Association for Dental Research Annual Sessions from 2004 to 2005 and Full-Text Publication

    PubMed Central

    Prasad, Soni; Lee, Damian J.; Yuan, Judy Chia-Chun; Barao, Valentim A. R.; Shyamsunder, Nodesh; Sukotjo, Cortino

    2012-01-01

    Purpose. The purpose of this study was to evaluate the discrepancies between abstracts presented at the IADR meeting (2004-2005) and their full-text publication. Material and Methods. Abstracts from the Prosthodontic Section of IADR meeting were obtained. The following information was collected: abstract title, number of authors, study design, statistical analysis, outcome, and funding source. PubMed was used to identify the full-text publication of the abstracts. The discrepancies between the abstract and the full-text publication were examined, categorized as major and minor discrepancies, and quantified. The data were collected and analyzed using descriptive analysis. Frequency and percentage of major and minor discrepancies were calculated. Results. A total of 109 (95.6%) articles showed changes from their abstracts. Seventy-four (65.0%) and 105 (92.0%) publications had at least one major and one minor discrepancies, respectively. Minor discrepancies were more prevalent (92.0%) than major discrepancies (65.0%). The most common minor discrepancy was observed in the title (80.7%), and most common major discrepancies were seen in results (48.2%). Conclusion. Minor discrepancies were more prevalent than major discrepancies. The data presented in this study may be useful to establish a more comprehensive structured abstract requirement for future meetings. PMID:22505912

  5. ICO amplicon NGS data analysis: a Web tool for variant detection in common high-risk hereditary cancer genes analyzed by amplicon GS Junior next-generation sequencing.

    PubMed

    Lopez-Doriga, Adriana; Feliubadaló, Lídia; Menéndez, Mireia; Lopez-Doriga, Sergio; Morón-Duran, Francisco D; del Valle, Jesús; Tornero, Eva; Montes, Eva; Cuesta, Raquel; Campos, Olga; Gómez, Carolina; Pineda, Marta; González, Sara; Moreno, Victor; Capellá, Gabriel; Lázaro, Conxi

    2014-03-01

    Next-generation sequencing (NGS) has revolutionized genomic research and is set to have a major impact on genetic diagnostics thanks to the advent of benchtop sequencers and flexible kits for targeted libraries. Among the main hurdles in NGS are the difficulty of performing bioinformatic analysis of the huge volume of data generated and the high number of false positive calls that could be obtained, depending on the NGS technology and the analysis pipeline. Here, we present the development of a free and user-friendly Web data analysis tool that detects and filters sequence variants, provides coverage information, and allows the user to customize some basic parameters. The tool has been developed to provide accurate genetic analysis of targeted sequencing of common high-risk hereditary cancer genes using amplicon libraries run in a GS Junior System. The Web resource is linked to our own mutation database, to assist in the clinical classification of identified variants. We believe that this tool will greatly facilitate the use of the NGS approach in routine laboratories. PMID:24227591

  6. Localization of causal locus in the genome of the brown macroalga Ectocarpus: NGS-based mapping and positional cloning approaches.

    PubMed

    Billoud, Bernard; Jouanno, Émilie; Nehr, Zofia; Carton, Baptiste; Rolland, Élodie; Chenivesse, Sabine; Charrier, Bénédicte

    2015-01-01

    Mutagenesis is the only process by which unpredicted biological gene function can be identified. Despite that several macroalgal developmental mutants have been generated, their causal mutation was never identified, because experimental conditions were not gathered at that time. Today, progresses in macroalgal genomics and judicious choices of suitable genetic models make mutated gene identification possible. This article presents a comparative study of two methods aiming at identifying a genetic locus in the brown alga Ectocarpus siliculosus: positional cloning and Next-Generation Sequencing (NGS)-based mapping. Once necessary preliminary experimental tools were gathered, we tested both analyses on an Ectocarpus morphogenetic mutant. We show how a narrower localization results from the combination of the two methods. Advantages and drawbacks of these two approaches as well as potential transfer to other macroalgae are discussed. PMID:25745426

  7. Localization of causal locus in the genome of the brown macroalga Ectocarpus: NGS-based mapping and positional cloning approaches

    PubMed Central

    Billoud, Bernard; Jouanno, Émilie; Nehr, Zofia; Carton, Baptiste; Rolland, Élodie; Chenivesse, Sabine; Charrier, Bénédicte

    2015-01-01

    Mutagenesis is the only process by which unpredicted biological gene function can be identified. Despite that several macroalgal developmental mutants have been generated, their causal mutation was never identified, because experimental conditions were not gathered at that time. Today, progresses in macroalgal genomics and judicious choices of suitable genetic models make mutated gene identification possible. This article presents a comparative study of two methods aiming at identifying a genetic locus in the brown alga Ectocarpus siliculosus: positional cloning and Next-Generation Sequencing (NGS)-based mapping. Once necessary preliminary experimental tools were gathered, we tested both analyses on an Ectocarpus morphogenetic mutant. We show how a narrower localization results from the combination of the two methods. Advantages and drawbacks of these two approaches as well as potential transfer to other macroalgae are discussed. PMID:25745426

  8. Annual Energy Review 2007

    SciTech Connect

    Seiferlein, Katherine E.

    2008-06-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....”

  9. International energy annual 1997

    SciTech Connect

    1999-04-01

    The International Energy Annual presents an overview of key international energy trends for production, consumption, imports, and exports of primary energy commodities in over 220 countries, dependencies, and areas of special sovereignty. Also included are population and gross domestic product data, as well as prices for crude oil and petroleum products in selected countries. Renewable energy reported in the International Energy Annual includes hydroelectric power and geothermal, solar, and wind electric power. Also included are biomass electric power for Brazil and the US, and biomass, geothermal, and solar energy produced in the US and not used for electricity generation. This report is published to keep the public and other interested parties fully informed of primary energy supplies on a global basis. The data presented have been largely derived from published sources. The data have been converted to units of measurement and thermal values (Appendices E and F) familiar to the American public. 93 tabs.

  10. Alberta Advanced Education Annual Report 2005-06

    ERIC Educational Resources Information Center

    Alberta Advanced Education, 2006

    2006-01-01

    The Public Accounts of Alberta are prepared in accordance with the Financial Administration Act and the "Government Accountability Act." The Public Accounts consist of the annual report of the Government of Alberta and the annual reports of each of the 24 ministries. The annual report of the Government of Alberta released June 26, 2006 contains…

  11. 41 CFR 105-69.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Semi-annual compilation... Administration 69-NEW RESTRICTIONS ON LOBBYING Agency Reports § 105-69.600 Semi-annual compilation. (a) The head... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  12. 45 CFR 1230.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Semi-annual compilation. 1230.600 Section 1230.600... SERVICE NEW RESTRICTIONS ON LOBBYING Agency Reports § 1230.600 Semi-annual compilation. (a) The head of... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  13. 45 CFR 1230.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Semi-annual compilation. 1230.600 Section 1230.600... SERVICE NEW RESTRICTIONS ON LOBBYING Agency Reports § 1230.600 Semi-annual compilation. (a) The head of... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  14. 45 CFR 1158.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Semi-annual compilation. 1158.600 Section 1158.600... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NEW RESTRICTIONS ON LOBBYING Agency Reports § 1158.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  15. 45 CFR 1230.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Semi-annual compilation. 1230.600 Section 1230.600... SERVICE NEW RESTRICTIONS ON LOBBYING Agency Reports § 1230.600 Semi-annual compilation. (a) The head of... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  16. 45 CFR 1158.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Semi-annual compilation. 1158.600 Section 1158.600... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NEW RESTRICTIONS ON LOBBYING Agency Reports § 1158.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  17. 45 CFR 1158.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Semi-annual compilation. 1158.600 Section 1158.600... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NEW RESTRICTIONS ON LOBBYING Agency Reports § 1158.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  18. 41 CFR 105-69.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 3 2013-07-01 2013-07-01 false Semi-annual compilation... Administration 69-NEW RESTRICTIONS ON LOBBYING Agency Reports § 105-69.600 Semi-annual compilation. (a) The head... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  19. 41 CFR 105-69.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 41 Public Contracts and Property Management 3 2012-01-01 2012-01-01 false Semi-annual compilation... Administration 69-NEW RESTRICTIONS ON LOBBYING Agency Reports § 105-69.600 Semi-annual compilation. (a) The head... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  20. 41 CFR 105-69.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false Semi-annual compilation... Administration 69-NEW RESTRICTIONS ON LOBBYING Agency Reports § 105-69.600 Semi-annual compilation. (a) The head... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  1. 45 CFR 1158.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Semi-annual compilation. 1158.600 Section 1158.600... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NEW RESTRICTIONS ON LOBBYING Agency Reports § 1158.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  2. 1987 annual report on low-level radioactive waste management progress: Report to Congress in response to Public Law 99-240

    SciTech Connect

    Not Available

    1988-08-01

    In response to Section 7(b) of the Low-Level Radioactive Waste Policy Amendments Act of 1985 (Public Law 99-240), this report summarizes the progress of states and low-level radioactive waste compacts in 1987 in establishing new low-level waste disposal facilities. It also reports the volume of low-level waste received for disposal in 1987 by commercially operated low-level waste disposal facilities.

  3. Petroleum marketing annual 1993

    SciTech Connect

    Not Available

    1995-01-01

    The Petroleum Marketing Annual (PMA) contains statistical data on a variety of crude oils and refined petroleum products. The publication provides statistics on crude oil costs and refined petroleum products sales for use by industry, government, private sector analysts, educational institutions, and consumers. Data on crude oil include the domestic first purchase price, the free-on-board (f.o.b.) and landed cost of imported crude oil, and the refiners acquisition cost of crude oil. Sales data for motor gasoline, distillates, residuals, aviation fuels, kerosene, and propane are presented. For this publication, all estimates have been recalculated since their earlier publication in the Petroleum Marketing Monthly (PMM). These calculations made use of additional data and corrections that were received after the PMM publication dates.

  4. Administration of the Radiation Control for Health and Safety Act of 1968, Public Law 90-602, (1987 annual report). Report for January-December 1987

    SciTech Connect

    Not Available

    1988-04-01

    This document is an annual report submitted to the President for transmittal to the Congress. The Food and Drug Administration, through its Center for Devices and Radiological Health, is responsible for the day-to-day administration of the Radiation Control for Health and Safety Act of 1968. The report provides a summary of the operations of the Center in carrying out that responsibility for calendar year 1987. In reviewing the operations of the CDRH as reported in the document, it should be kept in mind that the day-to-day administration of the Act is only part of the Center's function. Other responsibilities include the administration and enforcement of the 1976 Medical Device Amendments to the Federal Food, Drug, and Cosmetic Act (not covered in the report). Manufacturers of electronic products are required by 21 CFR 1002.20 to report accidental radiation occurrences to the CDRH. The Center no longer maintains a Radiation Incidents Registry, since accidental radiation occurrences are reported through the Device Experience Network (DEN) and through the requirements of the Medical Device Reporting (MDR) regulations.

  5. Annual report (1982) on the administration of the Radiation Control for Health and Safety Act of 1968 - Public Law 90-602, April 1, 1983. Report for 1 Jan 82-31 Dec 82

    SciTech Connect

    Not Available

    1983-04-01

    The Secretary of HHS is required by Subpart 3, Part F of Title III of the PHS Act; 42 USC 263b et seq. (Public Law 90-602) to submit an annual report to the President for transmittal to the Congress on or before April 1 on the administration of The Radiation Control for Health and Safety Act. The detailed information required to be included in the report is outlined in Section 360D of the Public Health Service Act. The Food and Drug Administration, through its National Center for Devices and Radiological Health, is responsible for the day-to-day administration of the Act. This report covers the detailed operation of the Agency in carrying out that responsibility for calendar year 1982. In reviewing the operations of the NCDRH as reported in this document, it should be kept in mind that the day-to-day administration of the Radiation Control for Health and Safety Act of 1968 is only a part of the functions of the Center; other activities, for example in nuclear medicine and medical applications and guidelines, are not included in this report.

  6. Teacher to Teacher: The Public's Take on Public Schools

    ERIC Educational Resources Information Center

    McElroy, Edward J.

    2004-01-01

    There is no shortage of opinions about what the schools are doing right--and where they fall short. One interesting portal into public opinion comes from the annual Phi Delta Kappa/Gallup Poll of the Public's Attitudes Toward the Public Schools. Released each fall, this poll has proven to be an important gauge of where the public stands on timely…

  7. Live genomics for pathogen monitoring in public health.

    PubMed

    D'Auria, Giuseppe; Schneider, Maria Victoria; Moya, Andrés

    2014-01-01

    Whole genome analysis based on next generation sequencing (NGS) now represents an affordable framework in public health systems. Robust analytical pipelines of genomic data provides in short laps of time (hours) information about taxonomy, comparative genomics (pan-genome) and single polymorphisms profiles. Pathogenic organisms of interest can be tracked at the genomic level, allowing monitoring at one-time several variables including: epidemiology, pathogenicity, resistance to antibiotics, virulence, persistence factors, mobile elements and adaptation features. Such information can be obtained not only at large spectra, but also at the "local" level, such as in the event of a recurrent or emergency outbreak. This paper reviews the state of the art in infection diagnostics in the context of modern NGS methodologies. We describe how actuation protocols in a public health environment will benefit from a "streaming approach" (pipeline). Such pipeline would NGS data quality assessment, data mining for comparative analysis, searching differential genetic features, such as virulence, resistance persistence factors and mutation profiles (SNPs and InDels) and formatted "comprehensible" results. Such analytical protocols will enable a quick response to the needs of locally circumscribed outbreaks, providing information on the causes of resistance and genetic tracking elements for rapid detection, and monitoring actuations for present and future occurrences. PMID:25437609

  8. Live Genomics for Pathogen Monitoring in Public Health

    PubMed Central

    D’Auria, Giuseppe; Schneider, Maria Victoria; Moya, Andrés

    2014-01-01

    Whole genome analysis based on next generation sequencing (NGS) now represents an affordable framework in public health systems. Robust analytical pipelines of genomic data provides in a short lapse of time (hours) information about taxonomy, comparative genomics (pan-genome) and single polymorphisms profiles. Pathogenic organisms of interest can be tracked at the genomic level, allowing monitoring at one-time several variables including: epidemiology, pathogenicity, resistance to antibiotics, virulence, persistence factors, mobile elements and adaptation features. Such information can be obtained not only at large spectra, but also at the “local” level, such as in the event of a recurrent or emergency outbreak. This paper reviews the state of the art in infection diagnostics in the context of modern NGS methodologies. We describe how actuation protocols in a public health environment will benefit from a “streaming approach” (pipeline). Such pipeline would include NGS data quality assessment, data mining for comparative analysis, searching differential genetic features, such as virulence, resistance persistence factors and mutation profiles (SNPs and InDels) and formatted “comprehensible” results. Such analytical protocols will enable a quick response to the needs of locally circumscribed outbreaks, providing information on the causes of resistance and genetic tracking elements for rapid detection, and monitoring actuations for present and future occurrences. PMID:25437609

  9. Annual report on the administration of the Radiation Control for health and Safety Act of 1968, Public Law 90-602, April 1, 1991. Rept. for Jan-Dec 90

    SciTech Connect

    Not Available

    1991-04-01

    The Secretary of Health and Human Services is required by Subpart 3, Part F of Title III of the Public Health Service Act; 42 USC 263b et seq. (Public Law 90-602) to submit an annual report to the President for transmittal to the Congress on or before April 1 on the administration of the Radiation Control for Health and Safety Act. The detailed information required in the report is outlined in Section 360D of the Public Health Service Act. The Food and Drug Administration, through its Center for Devices and Radiological Health, is responsible for the day-to-day administration of the Radiation Control for Health and Safety Act of 1968. The report provides a summary of the operations of the Center in carrying out that responsibility for calendar year 1990. In reviewing the operations of the Center for Devices and Radiological Health as reported in the document, it should be kept in mind that the day-to-day administration of the Act is only part of the Center's function. Other responsibilities include the administration and enforcement of the 1976 Medical Device Amendments to the Federal Food, Drug, and Cosmetic Act (not covered in the report). Manufacturers of electronic products are required by 21 CFR 1002.20 to report accidental radiation occurrences to the Center for Devices and Radiological Health. The Center no longer maintains a Radiation Incidents Registry, since accidental radiation occurrences are reported through the Device Experience Network (DEN) and through the requirements of the Medical Device Reporting (MDR) Regulations.

  10. Annual Energy Review 2009

    SciTech Connect

    Fichman, Barbara T.

    2010-08-01

    The Annual Energy Review (AER) is the U.S. Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are statistics on total energy production, consumption, trade, and energy prices; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, and international energy; financial and environment indicators; and data unit conversions. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding the content of the AER and other EIA publications.

  11. Annual Energy Review 2011

    SciTech Connect

    Fichman, Barbara T.

    2012-09-01

    The Annual Energy Review (AER) is the U.S. Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are statistics on total energy production, consumption, trade, and energy prices; overviews of petroleum, natural gas, coal, electricity, nuclear energy, and renewable energy; financial and environment indicators; and data unit conversions. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding the content of the AER and other EIA publications.

  12. Annual Energy Review 2005

    SciTech Connect

    Seiferlein, Katherine E.

    2006-07-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  13. Annual Energy Review 2004

    SciTech Connect

    Seiferlein, Katherine E.

    2005-08-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  14. Annual Energy Review 2006

    SciTech Connect

    Seiferlein, Katherine E.

    2007-06-01

    The Annual Energy Review (AER) is the Energy Information Administration's (EIA) primary report of annual historical energy statistics. For many series, data begin with the year 1949. Included are data on total energy production, consumption, and trade; overviews of petroleum, natural gas, coal, electricity, nuclear energy, renewable energy, international energy, as well as financial and environment indicators; and data unit conversion tables. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the EIA under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  15. Electric power annual 1992

    SciTech Connect

    Not Available

    1994-01-06

    The Electric Power Annual presents a summary of electric utility statistics at national, regional and State levels. The objective of the publication is to provide industry decisionmakers, government policymakers, analysts and the general public with historical data that may be used in understanding US electricity markets. The Electric Power Annual is prepared by the Survey Management Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); US Department of Energy. ``The US Electric Power Industry at a Glance`` section presents a profile of the electric power industry ownership and performance, and a review of key statistics for the year. Subsequent sections present data on generating capability, including proposed capability additions; net generation; fossil-fuel statistics; retail sales; revenue; financial statistics; environmental statistics; electric power transactions; demand-side management; and nonutility power producers. In addition, the appendices provide supplemental data on major disturbances and unusual occurrences in US electricity power systems. Each section contains related text and tables and refers the reader to the appropriate publication that contains more detailed data on the subject matter. Monetary values in this publication are expressed in nominal terms.

  16. LLNL NESHAP's 1999 Annual Report

    SciTech Connect

    Gallegos, G.; Biermann, A.H.; Harrach, R.J.; Bertoldo, N.A.; Berger, R.L.; Surano,K.A.

    2000-06-01

    This annual report is prepared pursuant to the National Emissions Standards for Hazardous Air Pollutants (NESHAPs) 40 CFR Part 61, Subpart H; Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an annual effective dose equivalent (EDE) of 10 mrem (100 {micro}Sv) to any member of the public. The EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from 1999 operations are summarized.

  17. 45 CFR 1158.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Semi-annual compilation. 1158.600 Section 1158.600 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NEW RESTRICTIONS ON LOBBYING Agency Reports § 1158.600 Semi-annual compilation. (a) The head of...

  18. 45 CFR 604.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Semi-annual compilation. 604.600 Section 604.600 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION NEW RESTRICTIONS ON LOBBYING Agency Reports § 604.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports...

  19. Robust species taxonomy assignment algorithm for 16S rRNA NGS reads: application to oral carcinoma samples

    PubMed Central

    Al-Hebshi, Nezar Noor; Nasher, Akram Thabet; Idris, Ali Mohamed; Chen, Tsute

    2015-01-01

    Background Usefulness of next-generation sequencing (NGS) in assessing bacteria associated with oral squamous cell carcinoma (OSCC) has been undermined by inability to classify reads to the species level. Objective The purpose of this study was to develop a robust algorithm for species-level classification of NGS reads from oral samples and to pilot test it for profiling bacteria within OSCC tissues. Methods Bacterial 16S V1-V3 libraries were prepared from three OSCC DNA samples and sequenced using 454's FLX chemistry. High-quality, well-aligned, and non-chimeric reads ≥350 bp were classified using a novel, multi-stage algorithm that involves matching reads to reference sequences in revised versions of the Human Oral Microbiome Database (HOMD), HOMD extended (HOMDEXT), and Greengene Gold (GGG) at alignment coverage and percentage identity ≥98%, followed by assignment to species level based on top hit reference sequences. Priority was given to hits in HOMD, then HOMDEXT and finally GGG. Unmatched reads were subject to operational taxonomic unit analysis. Results Nearly, 92.8% of the reads were matched to updated-HOMD 13.2, 1.83% to trusted-HOMDEXT, and 1.36% to modified-GGG. Of all matched reads, 99.6% were classified to species level. A total of 228 species-level taxa were identified, representing 11 phyla; the most abundant were Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Actinobacteria. Thirty-five species-level taxa were detected in all samples. On average, Prevotella oris, Neisseria flava, Neisseria flavescens/subflava, Fusobacterium nucleatum ss polymorphum, Aggregatibacter segnis, Streptococcus mitis, and Fusobacterium periodontium were the most abundant. Bacteroides fragilis, a species rarely isolated from the oral cavity, was detected in two samples. Conclusion This multi-stage algorithm maximizes the fraction of reads classified to the species level while ensuring reliable classification by giving priority to the human, oral reference

  20. 43 CFR 2524.2 - Annual proof.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Annual proof. 2524.2 Section 2524.2 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR LAND RESOURCE MANAGEMENT (2000) DESERT-LAND ENTRIES Desert-Land Entries Within...

  1. 43 CFR 2524.2 - Annual proof.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Annual proof. 2524.2 Section 2524.2 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR LAND RESOURCE MANAGEMENT (2000) DESERT-LAND ENTRIES Desert-Land Entries Within...

  2. 43 CFR 2524.2 - Annual proof.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Annual proof. 2524.2 Section 2524.2 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR LAND RESOURCE MANAGEMENT (2000) DESERT-LAND ENTRIES Desert-Land Entries Within...

  3. 43 CFR 2524.2 - Annual proof.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Annual proof. 2524.2 Section 2524.2 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR LAND RESOURCE MANAGEMENT (2000) DESERT-LAND ENTRIES Desert-Land Entries Within...

  4. Coal industry annual 1997

    SciTech Connect

    1998-12-01

    Coal Industry Annual 1997 provides comprehensive information about US coal production, number of mines, prices, productivity, employment, productive capacity, and recoverable reserves. US Coal production for 1997 and previous years is based on the annual survey EIA-7A, Coal Production Report. This report presents data on coal consumption, coal distribution, coal stocks, coal prices, and coal quality for Congress, Federal and State agencies, the coal industry, and the general public. Appendix A contains a compilation of coal statistics for the major coal-producing States. This report includes a national total coal consumption for nonutility power producers that are not in the manufacturing, agriculture, mining, construction, or commercial sectors. 14 figs., 145 tabs.

  5. 75 FR 12584 - Sunshine Act; Public Hearing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... CORPORATION Sunshine Act; Public Hearing March 17, 2010. OPIC's Sunshine Act notice of its Annual Public Hearing meeting was published in the Federal Register (Volume 75, Number 38, Pages 9004 and 9005) on... record; therefore, OPIC's annual public hearing scheduled for 2 p.m. on March 17, 2010 has been...

  6. What next-generation sequencing (NGS) technology has enabled us to learn about primary autosomal recessive microcephaly (MCPH).

    PubMed

    Morris-Rosendahl, Deborah J; Kaindl, Angela M

    2015-10-01

    The impact that next-generation sequencing technology (NGS) is having on many aspects of molecular and cell biology, is becoming increasingly apparent. One of the most noticeable outcomes of the new technology in human genetics, has been the accelerated rate of identification of disease-causing genes. Especially for rare, heterogeneous disorders, such as autosomal recessive primary microcephaly (MCPH), the handful of genes previously known to harbour disease-causing mutations, has grown at an unprecedented rate within a few years. Knowledge of new genes mutated in MCPH over the last four years has contributed to our understanding of the disorder at both the clinical and cellular levels. The functions of proteins such as WDR62, CASC5, PHC1, CDK6, CENP-E, CENP-F, CEP63, ZNF335, PLK4 and TUBGPC, have been added to the complex network of critical cellular processes known to be involved in brain growth and size. In addition to the importance of mitotic spindle assembly and structure, centrosome and centriole function and DNA repair and damage response, new mechanisms involving kinetochore-associated proteins and chromatin remodelling complexes have been elucidated. Two of the major contributions to our clinical knowledge are the realisation that primary microcephaly caused by mutations in genes at the MCPH loci is seldom an isolated clinical feature and is often accompanied either by additional cortical malformations or primordial dwarfism. Gene-phenotype correlations are being revisited, with a new dimension of locus heterogeneity and phenotypic variability being revealed. PMID:26050940

  7. Photometric calibration of NGS/POSS and ESO/SRC plates using the NOAO PDS measuring engine. I - Stellar photometry

    NASA Technical Reports Server (NTRS)

    Cutri, Roc M.; Low, Frank J.; Marvel, Kevin B.

    1992-01-01

    The PDS/Monet measuring engine at the National Optical Astronomy Observatory was used to obtain photometry of nearly 10,000 stars on the NGS/POSS and 2000 stars on the ESO/SRC Survey glass plates. These measurements have been used to show that global transformation functions exist that allow calibration of stellar photometry from any blue or red plate to equivalent Johnson B and Cousins R photoelectric magnitudes. The four transformation functions appropriate for the POSS O and E and ESO/SRC J and R plates were characterized, and it was found that, within the measurement uncertainties, they vary from plate to plate only by photometric zero-point offsets. A method is described to correct for the zero-point shifts and to obtain calibrated B and R photometry of stellar sources to an average accuracy of 0.3-0.4 mag within the range R between values of 8 and 19.5 for red plates in both surveys, B between values of 9 and 20.5 on POSS blue plates, and B between values of 10 and 20.5 on ESO/SRC blue plates. This calibration procedure makes it possible to obtain rapid photometry of very large numbers of stellar sources.

  8. Applying NGS Data to Find Evolutionary Network Biomarkers from the Early and Late Stages of Hepatocellular Carcinoma

    PubMed Central

    Wong, Yung-Hao; Wu, Chia-Chou; Lin, Chih-Lung; Chen, Ting-Shou; Chang, Tzu-Hao; Chen, Bor-Sen

    2015-01-01

    Hepatocellular carcinoma (HCC) is a major liver tumor (~80%), besides hepatoblastomas, angiosarcomas, and cholangiocarcinomas. In this study, we used a systems biology approach to construct protein-protein interaction networks (PPINs) for early-stage and late-stage liver cancer. By comparing the networks of these two stages, we found that the two networks showed some common mechanisms and some significantly different mechanisms. To obtain differential network structures between cancer and noncancer PPINs, we constructed cancer PPIN and noncancer PPIN network structures for the two stages of liver cancer by systems biology method using NGS data from cancer cells and adjacent noncancer cells. Using carcinogenesis relevance values (CRVs), we identified 43 and 80 significant proteins and their PPINs (network markers) for early-stage and late-stage liver cancer. To investigate the evolution of network biomarkers in the carcinogenesis process, a primary pathway analysis showed that common pathways of the early and late stages were those related to ordinary cancer mechanisms. A pathway specific to the early stage was the mismatch repair pathway, while pathways specific to the late stage were the spliceosome pathway, lysine degradation pathway, and progesterone-mediated oocyte maturation pathway. This study provides a new direction for cancer-targeted therapies at different stages. PMID:26366411

  9. Neurofibromatosis type 1 molecular diagnosis: what can NGS do for you when you have a large gene with loss of function mutations?

    PubMed Central

    Pasmant, Eric; Parfait, Béatrice; Luscan, Armelle; Goussard, Philippe; Briand-Suleau, Audrey; Laurendeau, Ingrid; Fouveaut, Corinne; Leroy, Chrystel; Montadert, Annelore; Wolkenstein, Pierre; Vidaud, Michel; Vidaud, Dominique

    2015-01-01

    Molecular diagnosis of neurofibromatosis type 1 (NF1) is challenging owing to the large size of the tumour suppressor gene NF1, and the lack of mutation hotspots. A somatic alteration of the wild-type NF1 allele is observed in NF1-associated tumours. Genetic heterogeneity in NF1 was confirmed in patients with SPRED1 mutations. Here, we present a targeted next-generation sequencing (NGS) of NF1 and SPRED1 using a multiplex PCR approach (230 amplicons of ∼150 bp) on a PGM sequencer. The chip capacity allowed mixing 48 bar-coded samples in a 4-day workflow. We validated the NGS approach by retrospectively testing 30 NF1-mutated samples, and then prospectively analysed 279 patients in routine diagnosis. On average, 98.5% of all targeted bases were covered by at least 20X and 96% by at least 100X. An NF1 or SPRED1 alteration was found in 246/279 (88%) and 10/279 (4%) patients, respectively. Genotyping throughput was increased over 10 times, as compared with Sanger, with ∼90€ for consumables per sample. Interestingly, our targeted NGS approach also provided quantitative information based on sequencing depth allowing identification of multiexons deletion or duplication. We then addressed the NF1 somatic mutation detection sensitivity in mosaic NF1 patients and tumours. PMID:25074460

  10. 45 CFR 1801.54 - Annual report.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Payment Conditions and Procedures § 1801.54 Annual report. (a) Scholars with remaining eligibility for scholarship stipends must submit no later than July 15 an annual report to...

  11. 45 CFR 1801.54 - Annual report.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Payment Conditions and Procedures § 1801.54 Annual report. (a) Scholars with remaining eligibility for scholarship stipends must submit no later than July 15 an annual report to...

  12. 45 CFR 1801.54 - Annual report.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Payment Conditions and Procedures § 1801.54 Annual report. (a) Scholars with remaining eligibility for scholarship stipends must submit no later than July 15 an annual report to...

  13. 45 CFR 1801.54 - Annual report.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Welfare Regulations Relating to Public Welfare (Continued) HARRY S. TRUMAN SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM Payment Conditions and Procedures § 1801.54 Annual report. (a) Scholars with remaining eligibility for scholarship stipends must submit no later than July 15 an annual report to...

  14. Strategic petroleum reserve annual report

    SciTech Connect

    1996-02-15

    Section 165 of the Energy Policy and Conservation Act (Public Law 94- 163), as amended, requires the Secretary of Energy to submit annual reports to the President and the Congress on activities of the Strategic Petroleum Reserve (SPR). This report describes activities for the year ending December 31, 1995.

  15. Annual report to Congress

    SciTech Connect

    1992-03-01

    This is the eighth annual report submitted by the Office of Civilian Radioactive Waste Management (OCRWM) to Congress. It covers activities and expenditures during Fiscal Year 1991, which ended September 30, 1991. Chapter 1 of this report describes OCRWM`s mission and objectives. Chapters 2 through 8 cover the following topics: earning public trust and confidence; geological disposal; monitored retrieval storage; transportation; systems integration and regulatory compliance; international programs; and program management. Financial statements for the Nuclear Waste Fund are presented in Chapter 9.

  16. Assessment of the Impact of a New Guanidine Suppressor In NGS on F/H Laboratory Analyses For DWPF and Saltstone MCU Transfers

    SciTech Connect

    Bannochie, C. J.

    2013-04-29

    Implementation of the Next Generation Solvent (NGS) in the Modular Caustic-Side Solvent Extraction Unit (MCU) will now proceed with a new suppressor compound, 1,2,3-tris(3,7-dimethyloctyl)guanidine (TiDG), replacing the originally planned suppressor for NGS, 1,3-dicyclohexyl-2-(11-methyldodecyl) guanidine (DCiTG). The Savannah River National Laboratory (SRNL) was tasked with evaluating the potential impact to F/H Laboratory analyses supporting the Defense Waste Processing Facility (DWPF) Waste Acceptance Criteria (WAC) used to qualify transfers of MCU Strip Effluent (SE) into the facility and the Saltstone WAC used to qualify transfers of Tank 50 containing Decontaminated Salt Solution (DSS) from MCU into Saltstone. This assigned scope is covered by a Task Technical and Quality Assurance Plan (TTQAP). Previous impact evaluations were conducted when the DCiTG suppressor was planned for NGS and concluded that there was no impact to either the determination of MCU SE pH nor the analysis of Isopar® L carryover in the MCU SE and DSS streams. SRNL reported on this series of cross-check studies between the SRNL and F/H Laboratories. The change in suppressor from DCiTG to TiDG in the NGS should not impact the measurement of Isopar® L or pH in SE or DSS necessary to satisfy DWPF and Saltstone WAC (Tank 50) criteria, respectively. A statistical study of the low bias observed in Isopar® L measurements in both SRNL and F/H Laboratories may be necessary now that the final NGS composition is fixed in order to quantify the low bias so that a proper correction can be applied to measurements critical to the DWPF and Saltstone WACs. Depending upon the final DWPF WAC requirement put in place for SE pH, it could become necessary to implement an alternative ICP-AES measurement of boron. The current blended solvent system testing in SRNL should address any impacts to Isopar® L carryover into either the DSS or the SE. It is recommended that SRNL monitor the current blended solvent

  17. Annual Energy Review 2002

    SciTech Connect

    Seiferlein, Katherine E.

    2003-10-01

    The Annual Energy Review (AER) presents the Energy Information Administration’s historical energy statistics. For many series, statistics are given for every year from 1949 through 2002. The statistics, expressed in either physical units or British thermal units, cover all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels, electricity, and renewable energy sources. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the Energy Information Administration (EIA) under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications. Related Publication: Readers of the AER may also be interested in EIA’s Monthly Energy Review, which presents monthly updates of many of the data in the AER. Contact our National Energy Information Center for more information.

  18. Uranium industry annual 1994

    SciTech Connect

    1995-07-05

    The Uranium Industry Annual 1994 (UIA 1994) provides current statistical data on the US uranium industry`s activities relating to uranium raw materials and uranium marketing during that survey year. The UIA 1994 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. It contains data for the 10-year period 1985 through 1994 as collected on the Form EIA-858, ``Uranium Industry Annual Survey.`` Data collected on the ``Uranium Industry Annual Survey`` (UIAS) provide a comprehensive statistical characterization of the industry`s activities for the survey year and also include some information about industry`s plans and commitments for the near-term future. Where aggregate data are presented in the UIA 1994, care has been taken to protect the confidentiality of company-specific information while still conveying accurate and complete statistical data. A feature article, ``Comparison of Uranium Mill Tailings Reclamation in the United States and Canada,`` is included in the UIA 1994. Data on uranium raw materials activities including exploration activities and expenditures, EIA-estimated resources and reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities, including purchases of uranium and enrichment services, and uranium inventories, enrichment feed deliveries (actual and projected), and unfilled market requirements are shown in Chapter 2.

  19. Coal industry annual 1993

    SciTech Connect

    Not Available

    1994-12-06

    Coal Industry Annual 1993 replaces the publication Coal Production (DOE/FIA-0125). This report presents additional tables and expanded versions of tables previously presented in Coal Production, including production, number of mines, Productivity, employment, productive capacity, and recoverable reserves. This report also presents data on coal consumption, coal distribution, coal stocks, coal prices, coal quality, and emissions for a wide audience including the Congress, Federal and State agencies, the coal industry, and the general public. In addition, Appendix A contains a compilation of coal statistics for the major coal-producing States. This report does not include coal consumption data for nonutility Power Producers who are not in the manufacturing, agriculture, mining, construction, or commercial sectors. This consumption is estimated to be 5 million short tons in 1993.

  20. Publicity and public relations

    NASA Technical Reports Server (NTRS)

    Fosha, Charles E.

    1990-01-01

    This paper addresses approaches to using publicity and public relations to meet the goals of the NASA Space Grant College. Methods universities and colleges can use to publicize space activities are presented.

  1. Changing of the Guard: Migrating 16S and ITS Diversity Profiling from 454 Onto Alternate NGS Platforms

    PubMed Central

    McNally, R.; Johnson, M.; Kasinadhuni, N.; Tinning, M.; McGrath, K.

    2014-01-01

    The Australian Genome Research Facility (AGRF) currently operates a microbial diversity profiling service on the 454 GS-FLX platform, targeting various regions of 16S and ITS microbial genes. The recent announcement by Roche to discontinue support prompted us to investigate how this service can be transitioned to alternate NGS platforms. The objective of this study was to evaluate the accuracy and reproducibility of each platform/target combination for both artificial control samples (known gDNA pools) and natural microbial community samples (soils). Replicate PCR amplicons were generated for several microbial and fungal regions (16S and ITS), and sequenced on the GS-FLX, MiSeq and Ion Torrent PGM platforms. The resulting sequences were identified at different taxonomic levels (phylum to species) and the results for each sample, platform and target compiled. This comparison revealed distinct and reproducible differences between the same samples run on different platforms. While no platform was able to perfectly describe the proportions of the artificial pooled sample, each one showed a high level of reproducibility (< 1% variance). This suggests that PCR bias during amplification gives rise to consistent biases in resulting microbial distributions and confirms this method as a useful tool for comparing samples and monitoring changes, but demonstrate its weakness for absolute quantitation. Between platforms, the long reads of the GS-FLX gave the greatest resolution for microbial identification, followed by the MiSeq. Despite the shorter amplicon size, the ion torrent was still able to provide high resolution profiles of the microbial samples. These results show that currently, the 454 still provides higher resolution data sets for microbial diversity than other available platforms. However, as read lengths improve and error rates decrease, these alternate platforms should provide a suitable transition pathway for researchers interested in profiling microbial

  2. Identification and Characterization of Microsatellite Loci in Maqui (Aristotelia chilensis [Molina] Stunz) Using Next-Generation Sequencing (NGS)

    PubMed Central

    Bastías, Adriana; Correa, Francisco; Rojas, Pamela; Almada, Rubén; Muñoz, Carlos; Sagredo, Boris

    2016-01-01

    Maqui (Aristotelia chilensis [Molina] Stunz) is a small dioecious tree native to South America with edible fruit characterized by very high antioxidant capacity and anthocyanin content. To preserve maqui as a genetic resource it is essential to study its genetic diversity. However, the complete genome is unknown and only a few gene sequences are available in databases. Simple sequence repeats (SSR) markers, which are neutral, co-dominant, reproducible and highly variable, are desirable to support genetic studies in maqui populations. By means of identification and characterization of microsatellite loci from a maqui genotype, using 454 sequencing technology, we develop a set of SSR for this species. Obtaining a total of 165,043 shotgun genome sequences, with an average read length of 387 bases, we covered 64 Mb of the maqui genome. Reads were assembled into 4,832 contigs, while 98,546 reads remained as singletons, generating a total of 103,378 consensus genomic sequences. A total of 24,494 SSR maqui markers were identified. Of them, 15,950 SSR maqui markers were classified as perfects. The most common SSR motifs were dinucleotide (31%), followed by tetranucleotide (26%) and trinucleotide motifs (24%). The motif AG/CT (28.4%) was the most abundant, while the motif AC (89 bp) was the largest. Eleven polymorphic SSRs were selected and used to analyze a population of 40 maqui genotypes. Polymorphism information content (PIC) ranged from 0.117 to 0.82, with an average of 0.58. Non-significant groups were observed in the maqui population, showing a panmictic genetic structure. In addition, we also predicted 11150 putative genes and 3 microRNAs (miRNAs) in maqui sequences. This results, including partial sequences of genes, some miRNAs and SSR markers from high throughput next generation sequencing (NGS) of maqui genomic DNA, constitute the first platform to undertake genetic and molecular studies of this important species. PMID:27459734

  3. Identification and Characterization of Microsatellite Loci in Maqui (Aristotelia chilensis [Molina] Stunz) Using Next-Generation Sequencing (NGS).

    PubMed

    Bastías, Adriana; Correa, Francisco; Rojas, Pamela; Almada, Rubén; Muñoz, Carlos; Sagredo, Boris

    2016-01-01

    Maqui (Aristotelia chilensis [Molina] Stunz) is a small dioecious tree native to South America with edible fruit characterized by very high antioxidant capacity and anthocyanin content. To preserve maqui as a genetic resource it is essential to study its genetic diversity. However, the complete genome is unknown and only a few gene sequences are available in databases. Simple sequence repeats (SSR) markers, which are neutral, co-dominant, reproducible and highly variable, are desirable to support genetic studies in maqui populations. By means of identification and characterization of microsatellite loci from a maqui genotype, using 454 sequencing technology, we develop a set of SSR for this species. Obtaining a total of 165,043 shotgun genome sequences, with an average read length of 387 bases, we covered 64 Mb of the maqui genome. Reads were assembled into 4,832 contigs, while 98,546 reads remained as singletons, generating a total of 103,378 consensus genomic sequences. A total of 24,494 SSR maqui markers were identified. Of them, 15,950 SSR maqui markers were classified as perfects. The most common SSR motifs were dinucleotide (31%), followed by tetranucleotide (26%) and trinucleotide motifs (24%). The motif AG/CT (28.4%) was the most abundant, while the motif AC (89 bp) was the largest. Eleven polymorphic SSRs were selected and used to analyze a population of 40 maqui genotypes. Polymorphism information content (PIC) ranged from 0.117 to 0.82, with an average of 0.58. Non-significant groups were observed in the maqui population, showing a panmictic genetic structure. In addition, we also predicted 11150 putative genes and 3 microRNAs (miRNAs) in maqui sequences. This results, including partial sequences of genes, some miRNAs and SSR markers from high throughput next generation sequencing (NGS) of maqui genomic DNA, constitute the first platform to undertake genetic and molecular studies of this important species. PMID:27459734

  4. 42 CFR 483.114 - Annual review of NF residents.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 5 2011-10-01 2011-10-01 false Annual review of NF residents. 483.114 Section 483... Screening and Annual Review of Mentally Ill and Mentally Retarded Individuals § 483.114 Annual review of NF... physical and mental condition, the resident requires— (1) The level of services provided by— (i) A NF;...

  5. 42 CFR 483.114 - Annual review of NF residents.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 5 2014-10-01 2014-10-01 false Annual review of NF residents. 483.114 Section 483... Screening and Annual Review of Mentally Ill and Mentally Retarded Individuals § 483.114 Annual review of NF... physical and mental condition, the resident requires— (1) The level of services provided by— (i) A NF;...

  6. 42 CFR 483.114 - Annual review of NF residents.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 5 2012-10-01 2012-10-01 false Annual review of NF residents. 483.114 Section 483... Screening and Annual Review of Mentally Ill and Mentally Retarded Individuals § 483.114 Annual review of NF... physical and mental condition, the resident requires— (1) The level of services provided by— (i) A NF;...

  7. 49 CFR 20.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false Semi-annual compilation. 20.600 Section 20.600... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  8. 34 CFR 82.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 1 2013-07-01 2013-07-01 false Semi-annual compilation. 82.600 Section 82.600....600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  9. 7 CFR 3018.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 15 2012-01-01 2012-01-01 false Semi-annual compilation. 3018.600 Section 3018.600..., DEPARTMENT OF AGRICULTURE NEW RESTRICTIONS ON LOBBYING Agency Reports § 3018.600 Semi-annual compilation. (a... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  10. 44 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 44 Emergency Management and Assistance 1 2013-10-01 2013-10-01 false Semi-annual compilation. 18... OF HOMELAND SECURITY GENERAL NEW RESTRICTIONS ON LOBBYING Agency Reports § 18.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  11. 22 CFR 311.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Semi-annual compilation. 311.600 Section 311.600 Foreign Relations PEACE CORPS NEW RESTRICTIONS ON LOBBYING Agency Reports § 311.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  12. 7 CFR 3018.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false Semi-annual compilation. 3018.600 Section 3018.600..., DEPARTMENT OF AGRICULTURE NEW RESTRICTIONS ON LOBBYING Agency Reports § 3018.600 Semi-annual compilation. (a... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  13. 45 CFR 93.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 1 2011-10-01 2011-10-01 false Semi-annual compilation. 93.600 Section 93.600... LOBBYING Agency Reports § 93.600 Semi-annual compilation. (a) The head of each agency shall collect and... first semi-annual compilation shall be submitted on May 31, 1990, and shall contain a compilation of...

  14. 44 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 44 Emergency Management and Assistance 1 2011-10-01 2011-10-01 false Semi-annual compilation. 18... OF HOMELAND SECURITY GENERAL NEW RESTRICTIONS ON LOBBYING Agency Reports § 18.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  15. 10 CFR 601.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Semi-annual compilation. 601.600 Section 601.600 Energy... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  16. 32 CFR 28.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 1 2013-07-01 2013-07-01 false Semi-annual compilation. 28.600 Section 28.600... REGULATIONS NEW RESTRICTIONS ON LOBBYING Agency Reports § 28.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  17. 49 CFR 20.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false Semi-annual compilation. 20.600 Section 20.600... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  18. 32 CFR 28.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false Semi-annual compilation. 28.600 Section 28.600... REGULATIONS NEW RESTRICTIONS ON LOBBYING Agency Reports § 28.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  19. 45 CFR 604.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Semi-annual compilation. 604.600 Section 604.600... RESTRICTIONS ON LOBBYING Agency Reports § 604.600 Semi-annual compilation. (a) The head of each agency shall... first semi-annual compilation shall be submitted on May 31, 1990, and shall contain a compilation of...

  20. 49 CFR 20.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false Semi-annual compilation. 20.600 Section 20.600... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  1. 22 CFR 519.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 2 2012-04-01 2009-04-01 true Semi-annual compilation. 519.600 Section 519.600... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  2. 38 CFR 45.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Semi-annual compilation... (CONTINUED) NEW RESTRICTIONS ON LOBBYING Agency Reports § 45.600 Semi-annual compilation. (a) The head of... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  3. 29 CFR 93.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 1 2013-07-01 2013-07-01 false Semi-annual compilation. 93.600 Section 93.600 Labor Office of the Secretary of Labor NEW RESTRICTIONS ON LOBBYING Agency Reports § 93.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  4. 24 CFR 87.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Semi-annual compilation. 87.600... Development NEW RESTRICTIONS ON LOBBYING Agency Reports § 87.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  5. 22 CFR 311.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 2 2013-04-01 2009-04-01 true Semi-annual compilation. 311.600 Section 311.600 Foreign Relations PEACE CORPS NEW RESTRICTIONS ON LOBBYING Agency Reports § 311.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  6. 45 CFR 93.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 1 2013-10-01 2013-10-01 false Semi-annual compilation. 93.600 Section 93.600... LOBBYING Agency Reports § 93.600 Semi-annual compilation. (a) The head of each agency shall collect and... first semi-annual compilation shall be submitted on May 31, 1990, and shall contain a compilation of...

  7. 22 CFR 227.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Semi-annual compilation. 227.600 Section 227... § 227.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  8. 24 CFR 87.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Semi-annual compilation. 87.600... Development NEW RESTRICTIONS ON LOBBYING Agency Reports § 87.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  9. 34 CFR 82.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 1 2011-07-01 2011-07-01 false Semi-annual compilation. 82.600 Section 82.600....600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  10. 40 CFR 34.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Semi-annual compilation. 34.600 Section... NEW RESTRICTIONS ON LOBBYING Agency Reports § 34.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  11. 44 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Semi-annual compilation. 18... OF HOMELAND SECURITY GENERAL NEW RESTRICTIONS ON LOBBYING Agency Reports § 18.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  12. 22 CFR 311.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Semi-annual compilation. 311.600 Section 311.600 Foreign Relations PEACE CORPS NEW RESTRICTIONS ON LOBBYING Agency Reports § 311.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  13. 22 CFR 138.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Semi-annual compilation. 138.600 Section 138... § 138.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  14. 49 CFR 20.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Semi-annual compilation. 20.600 Section 20.600... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  15. 38 CFR 45.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Semi-annual compilation... (CONTINUED) NEW RESTRICTIONS ON LOBBYING Agency Reports § 45.600 Semi-annual compilation. (a) The head of... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  16. 22 CFR 519.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Semi-annual compilation. 519.600 Section 519.600... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  17. 38 CFR 45.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Semi-annual compilation... (CONTINUED) NEW RESTRICTIONS ON LOBBYING Agency Reports § 45.600 Semi-annual compilation. (a) The head of... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  18. 24 CFR 87.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Semi-annual compilation. 87.600... Development NEW RESTRICTIONS ON LOBBYING Agency Reports § 87.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  19. 7 CFR 3018.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Semi-annual compilation. 3018.600 Section 3018.600..., DEPARTMENT OF AGRICULTURE NEW RESTRICTIONS ON LOBBYING Agency Reports § 3018.600 Semi-annual compilation. (a... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  20. 45 CFR 604.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Semi-annual compilation. 604.600 Section 604.600... RESTRICTIONS ON LOBBYING Agency Reports § 604.600 Semi-annual compilation. (a) The head of each agency shall... first semi-annual compilation shall be submitted on May 31, 1990, and shall contain a compilation of...

  1. 32 CFR 28.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 1 2012-07-01 2012-07-01 false Semi-annual compilation. 28.600 Section 28.600... REGULATIONS NEW RESTRICTIONS ON LOBBYING Agency Reports § 28.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  2. 45 CFR 604.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Semi-annual compilation. 604.600 Section 604.600... RESTRICTIONS ON LOBBYING Agency Reports § 604.600 Semi-annual compilation. (a) The head of each agency shall... first semi-annual compilation shall be submitted on May 31, 1990, and shall contain a compilation of...

  3. 22 CFR 519.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Semi-annual compilation. 519.600 Section 519.600... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  4. 22 CFR 227.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Semi-annual compilation. 227.600 Section 227... § 227.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  5. 40 CFR 34.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Semi-annual compilation. 34.600 Section... NEW RESTRICTIONS ON LOBBYING Agency Reports § 34.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  6. 44 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 44 Emergency Management and Assistance 1 2012-10-01 2011-10-01 true Semi-annual compilation. 18... OF HOMELAND SECURITY GENERAL NEW RESTRICTIONS ON LOBBYING Agency Reports § 18.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  7. 24 CFR 87.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Semi-annual compilation. 87.600... Development NEW RESTRICTIONS ON LOBBYING Agency Reports § 87.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  8. 38 CFR 45.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2013-07-01 2013-07-01 false Semi-annual compilation... (CONTINUED) NEW RESTRICTIONS ON LOBBYING Agency Reports § 45.600 Semi-annual compilation. (a) The head of... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  9. 22 CFR 138.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Semi-annual compilation. 138.600 Section 138... § 138.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  10. 7 CFR 3018.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Semi-annual compilation. 3018.600 Section 3018.600..., DEPARTMENT OF AGRICULTURE NEW RESTRICTIONS ON LOBBYING Agency Reports § 3018.600 Semi-annual compilation. (a... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  11. 22 CFR 311.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 2 2012-04-01 2009-04-01 true Semi-annual compilation. 311.600 Section 311.600 Foreign Relations PEACE CORPS NEW RESTRICTIONS ON LOBBYING Agency Reports § 311.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  12. 29 CFR 93.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 1 2011-07-01 2011-07-01 false Semi-annual compilation. 93.600 Section 93.600 Labor Office of the Secretary of Labor NEW RESTRICTIONS ON LOBBYING Agency Reports § 93.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  13. 40 CFR 34.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Semi-annual compilation. 34.600 Section... NEW RESTRICTIONS ON LOBBYING Agency Reports § 34.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  14. 29 CFR 93.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Semi-annual compilation. 93.600 Section 93.600 Labor Office of the Secretary of Labor NEW RESTRICTIONS ON LOBBYING Agency Reports § 93.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  15. 40 CFR 34.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Semi-annual compilation. 34.600 Section... NEW RESTRICTIONS ON LOBBYING Agency Reports § 34.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  16. 10 CFR 601.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Semi-annual compilation. 601.600 Section 601.600 Energy... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  17. 49 CFR 20.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 1 2014-10-01 2014-10-01 false Semi-annual compilation. 20.600 Section 20.600... Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure reports... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  18. 22 CFR 138.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Semi-annual compilation. 138.600 Section 138... § 138.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  19. 29 CFR 93.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 1 2014-07-01 2013-07-01 true Semi-annual compilation. 93.600 Section 93.600 Labor Office of the Secretary of Labor NEW RESTRICTIONS ON LOBBYING Agency Reports § 93.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  20. 45 CFR 604.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Semi-annual compilation. 604.600 Section 604.600... RESTRICTIONS ON LOBBYING Agency Reports § 604.600 Semi-annual compilation. (a) The head of each agency shall... first semi-annual compilation shall be submitted on May 31, 1990, and shall contain a compilation of...

  1. 38 CFR 45.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2014-07-01 2014-07-01 false Semi-annual compilation... (CONTINUED) NEW RESTRICTIONS ON LOBBYING Agency Reports § 45.600 Semi-annual compilation. (a) The head of... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  2. 22 CFR 227.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Semi-annual compilation. 227.600 Section 227... § 227.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  3. 44 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 44 Emergency Management and Assistance 1 2014-10-01 2014-10-01 false Semi-annual compilation. 18... OF HOMELAND SECURITY GENERAL NEW RESTRICTIONS ON LOBBYING Agency Reports § 18.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  4. 22 CFR 311.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 2 2014-04-01 2014-04-01 false Semi-annual compilation. 311.600 Section 311.600 Foreign Relations PEACE CORPS NEW RESTRICTIONS ON LOBBYING Agency Reports § 311.600 Semi-annual... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  5. 7 CFR 3018.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false Semi-annual compilation. 3018.600 Section 3018.600..., DEPARTMENT OF AGRICULTURE NEW RESTRICTIONS ON LOBBYING Agency Reports § 3018.600 Semi-annual compilation. (a... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  6. 24 CFR 87.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Semi-annual compilation. 87.600... Development NEW RESTRICTIONS ON LOBBYING Agency Reports § 87.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  7. 45 CFR 93.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 1 2014-10-01 2014-10-01 false Semi-annual compilation. 93.600 Section 93.600... LOBBYING Agency Reports § 93.600 Semi-annual compilation. (a) The head of each agency shall collect and... first semi-annual compilation shall be submitted on May 31, 1990, and shall contain a compilation of...

  8. 34 CFR 82.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 1 2014-07-01 2014-07-01 false Semi-annual compilation. 82.600 Section 82.600....600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  9. 22 CFR 519.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 2 2014-04-01 2014-04-01 false Semi-annual compilation. 519.600 Section 519... § 519.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the disclosure... information shall not be available for public inspection. (e) The first semi-annual compilation shall...

  10. 32 CFR 28.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 1 2014-07-01 2014-07-01 false Semi-annual compilation. 28.600 Section 28.600... REGULATIONS NEW RESTRICTIONS ON LOBBYING Agency Reports § 28.600 Semi-annual compilation. (a) The head of each... available for public inspection. (e) The first semi-annual compilation shall be submitted on May 31,...

  11. 77 FR 26585 - Annual Public Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-04

    ... provide an overview of its history, project updates, as well as projected activities going forward. DATES... has announced initial projects including: An evaluation of a systems biology approach to preclinical... providing an overview of its history, project updates, as well as projected activities going forward....

  12. 78 FR 23960 - Annual Public Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-23

    ... postmarket safety surveillance; the Systems Toxicology Project, an evaluation of a systems biology approach... provide an overview of its history, project updates, as well as projected activities going forward. DATES...) Project, looking at new ways to develop tuberculosis combination therapies. The Foundation seeks...

  13. Uranium industry annual 1995

    SciTech Connect

    1996-05-01

    The Uranium Industry Annual 1995 (UIA 1995) provides current statistical data on the U.S. uranium industry`s activities relating to uranium raw materials and uranium marketing. The UIA 1995 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. It contains data for the period 1986 through 2005 as collected on the Form EIA-858, ``Uranium Industry Annual Survey``. Data collected on the ``Uranium Industry Annual Survey`` provide a comprehensive statistical characterization of the industry`s plans and commitments for the near-term future. Where aggregate data are presented in the UIA 1995, care has been taken to protect the confidentiality of company-specific information while still conveying accurate and complete statistical data. Data on uranium raw materials activities for 1986 through 1995 including exploration activities and expenditures, EIA-estimated reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities for 1994 through 2005, including purchases of uranium and enrichment services, enrichment feed deliveries, uranium fuel assemblies, filled and unfilled market requirements, uranium imports and exports, and uranium inventories are shown in Chapter 2. The methodology used in the 1995 survey, including data edit and analysis, is described in Appendix A. The methodologies for estimation of resources and reserves are described in Appendix B. A list of respondents to the ``Uranium Industry Annual Survey`` is provided in Appendix C. For the reader`s convenience, metric versions of selected tables from Chapters 1 and 2 are presented in Appendix D along with the standard conversion factors used. A glossary of technical terms is at the end of the report. 14 figs., 56 tabs.

  14. Annual Energy Review 1993

    SciTech Connect

    Not Available

    1994-07-14

    This twelfth edition of the Annual Energy Review (AER) presents the Energy Information Administration`s historical energy statistics. For most series, statistics are given for every year from 1949 through 1993. Because coverage spans four and a half decades, the statistics in this report are well-suited to long-term trend analyses. The AER is comprehensive. It covers all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels and electricity. The AER also presents Energy Information Administration (EIA) statistics on some renewable energy sources. EIA estimates that its consumption series include about half of the renewable energy used in the United States. For a more complete discussion of EIA`s renewables data, see p. xix, ``Introducing Expanded Coverage of Renewable Energy Data Into the Historical Consumption Series.`` Copies of the 1993 edition of the Annual Energy Review may be obtained by using the order form in the back of this publication. Most of the data in the 1993 edition also are available on personal computer diskette. For more information about the diskettes, see the back of this publication. In addition, the data are available as part of the National Economic, Social, and Environmental Data Bank on a CD-ROM. For more information about the data bank, contact the US Department of Commerce Economics and Statistics Administration, on 202-482-1986.

  15. USGS Annual Water Data Reports

    SciTech Connect

    2012-04-01

    Water resources data are published annually for use by engineers, scientists, managers, educators, and the general public. These archival products supplement direct access to current and historical water data provided by the National Water Information System (NWIS). Beginning with Water Year 2006, annual water data reports are available as individual electronic Site Data Sheets for the entire Nation for retrieval, download, and localized printing on demand. National distribution includes tabular and map interfaces for search, query, display and download of data. Data provided include extreme and mean discharge rates.

  16. Ames research center publications, 1975

    NASA Technical Reports Server (NTRS)

    Sherwood, B. R. (Compiler)

    1977-01-01

    This bibliography cites 851 documents by Ames Research Center personnel and contractors which appeared in formal NASA publications, journals, books, patents, and contractor reports in 1975, or not included in previous annual bibliographies. An author index is provided.

  17. Celebrating Leadership in Public Health and Medicine

    MedlinePlus

    ... Home Current Issue Past Issues Celebrating Leadership in Public Health and Medicine Friends of the National Library of ... 2009 FNLM Annual Awards Dinner celebrated advances in public health and medicine, along with the individuals and organizations ...

  18. Annual Energy Review 2000

    SciTech Connect

    Seiferlein, Katherine E.

    2001-08-01

    The Annual Energy Review (AER) presents the Energy Information Administration’s historical energy statistics. For many series, statistics are given for every year from 1949 through 2000. The statistics, expressed in either physical units or British thermal units, cover all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels, electricity, and renewable energy sources. Publication of this report is required under Public Law 95–91 (Department of Energy Organization Act), Section 205(c), and is in keeping with responsibilities given to the Energy Information Administration under Section 205(a)(2), which states: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  19. Annual Energy Review 1997

    SciTech Connect

    Seiferlein, Katherine E.

    1998-07-01

    The Annual Energy Review (AER) presents the Energy Information Administration’s historical energy statistics. For many series, statistics are given for every year from 1949 through 1997. The statistics, expressed in either physical units or British thermal units, cover all major energy activities, including consumption, production, trade, stocks, and prices, for all major energy commodities, including fossil fuels, electricity, and renewable energy sources. Publication of this report is in keeping with responsibilities given to the Energy Information Administration (EIA) in Public Law 95–91 (Department of Energy Organization Act), which states, in part, in Section 205(a)(2) that: “The Administrator shall be responsible for carrying out a central, comprehensive, and unified energy data and information program which will collect, evaluate, assemble, analyze, and disseminate data and information....” The AER is intended for use by Members of Congress, Federal and State agencies, energy analysts, and the general public. EIA welcomes suggestions from readers regarding data series in the AER and in other EIA publications.

  20. Statistics & Input-Output Measures for Colorado Public Libraries, 1999.

    ERIC Educational Resources Information Center

    Colorado State Dept. of Education, Denver. State Library and Adult Education Office.

    "Statistics and Input-Output Measures for Colorado Public Libraries, 1999" is a compilation of data collected from the Colorado Public Library Annual Report sent to each public library jurisdiction in February of 2000 and returned in March. The 1999 response rate was excellent: 100% of Colorado public libraries returned the Annual Report and there…

  1. 45 CFR 93.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Semi-annual compilation. 93.600 Section 93.600 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION NEW RESTRICTIONS ON... first semi-annual compilation shall be submitted on May 31, 1990, and shall contain a compilation of...

  2. 43 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Semi-annual compilation. 18.600 Section 18... Reports § 18.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the... committees. Such information shall not be available for public inspection. (e) The first...

  3. 43 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false Semi-annual compilation. 18.600 Section 18... Reports § 18.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the... committees. Such information shall not be available for public inspection. (e) The first...

  4. 43 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true Semi-annual compilation. 18.600 Section 18... Reports § 18.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the... committees. Such information shall not be available for public inspection. (e) The first...

  5. 43 CFR 18.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false Semi-annual compilation. 18.600 Section 18... Reports § 18.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the... committees. Such information shall not be available for public inspection. (e) The first...

  6. Annual Report.

    ERIC Educational Resources Information Center

    Tellstrom, A. Theodore

    1978-01-01

    This Music Educators National Conference (MENC) 1977-78 report covers the following areas: audit, the government relations program, committees and commissions, the Music Industry Council, publications, student chapters, Music in Our Schools Week 1978, Presidential Scholars Program, MENC Historical Center, the National Inservice Conference, and the…

  7. Development and application of a next-generation-sequencing (NGS) approach to detect known and novel gene defects underlying retinal diseases

    PubMed Central

    2012-01-01

    Background Inherited retinal disorders are clinically and genetically heterogeneous with more than 150 gene defects accounting for the diversity of disease phenotypes. So far, mutation detection was mainly performed by APEX technology and direct Sanger sequencing of known genes. However, these methods are time consuming, expensive and unable to provide a result if the patient carries a new gene mutation. In addition, multiplicity of phenotypes associated with the same gene defect may be overlooked. Methods To overcome these challenges, we designed an exon sequencing array to target 254 known and candidate genes using Agilent capture. Subsequently, 20 DNA samples from 17 different families, including four patients with known mutations were sequenced using Illumina Genome Analyzer IIx next-generation-sequencing (NGS) platform. Different filtering approaches were applied to identify the genetic defect. The most likely disease causing variants were analyzed by Sanger sequencing. Co-segregation and sequencing analysis of control samples validated the pathogenicity of the observed variants. Results The phenotype of the patients included retinitis pigmentosa, congenital stationary night blindness, Best disease, early-onset cone dystrophy and Stargardt disease. In three of four control samples with known genotypes NGS detected the expected mutations. Three known and five novel mutations were identified in NR2E3, PRPF3, EYS, PRPF8, CRB1, TRPM1 and CACNA1F. One of the control samples with a known genotype belongs to a family with two clinical phenotypes (Best and CSNB), where a novel mutation was identified for CSNB. In six families the disease associated mutations were not found, indicating that novel gene defects remain to be identified. Conclusions In summary, this unbiased and time-efficient NGS approach allowed mutation detection in 75% of control cases and in 57% of test cases. Furthermore, it has the possibility of associating known gene defects with novel phenotypes

  8. NGS-based BRCA1/2 mutation testing of high-grade serous ovarian cancer tissue: results and conclusions of the first international round robin trial.

    PubMed

    Endris, Volker; Stenzinger, Albrecht; Pfarr, Nicole; Penzel, Roland; Möbs, Markus; Lenze, Dido; Darb-Esfahani, Silvia; Hummel, Michael; Sabine-Merkelbach-Bruse; Jung, Andreas; Lehmann, Ulrich; Kreipe, Hans; Kirchner, Thomas; Büttner, Reinhard; Jochum, Wolfram; Höfler, Gerald; Dietel, Manfred; Weichert, Wilko; Schirmacher, Peter

    2016-06-01

    With the approval of olaparib as monotherapy treatment in platinum-sensitive, relapsed high-grade serous ovarian cancer by the European Medical Agency (EMA), comprehensive genotyping of BRCA1 and BRCA2 in tumor tissue has become a mandatory pre-therapeutic test. This requires significant advances in routine tumor test methodologies due to the large size of both genes and the lack of mutational hot spots. Classical focused screening approaches, like Sanger sequencing, do not allow for a sensitive, rapid, and economic analysis of tumor tissue. Next-generation sequencing (NGS) approaches employing targeted panels for BRCA1/2 to interrogate formalin-fixed and paraffin-embedded tumor samples from either surgical resection or biopsy specimens can overcome these limitations. Although focused NGS methods have been implemented by few centers in routine molecular diagnostics for the analysis of some druggable oncogenic mutations, the reliable diagnostic testing of the entire coding regions of BRCA1 and BRCA2 was a new challenge requiring extensive technological improvement and quality management. Here, we describe the implementation and results of the first round robin trial for BRCA1/2 mutation testing in tumor tissue that was conducted in central Europe on May 2015, shortly after the approval and prior to the official release of olaparib. The high success rate of 81 % (21/26 test centers) demonstrates that BRCA1/2 multicenter mutation testing is well feasible in FFPE tumor tissue, extending to other tumor entities beyond ovarian cancer. The high number of test centers passing the trial demonstrates the success of the concerted efforts by German, Swiss, and Austrian pathology centers to ensure quality-controlled NGS-based testing and proves the potential of this technology in routine molecular pathology. On the basis of our results, we provide recommendations for predictive testing of tumor tissue for BRCA1/2 to clinical decision making in ovarian cancer patients. PMID

  9. 36 CFR 330.8 - Annual report.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Annual report. 330.8 Section 330.8 Parks, Forests, and Public Property CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY REGULATION OF LAW ENFORCEMENT SERVICES CONTRACTS AT CIVIL WORKS WATER RESOURCE PROJECTS ADMINISTERED BY THE CHIEF OF...

  10. 42 CFR 1007.17 - Annual report.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 5 2013-10-01 2013-10-01 false Annual report. 1007.17 Section 1007.17 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OIG AUTHORITIES... abuse and neglect of patients in health care facilities; the number of such complaints investigated...

  11. 42 CFR 1007.17 - Annual report.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 5 2011-10-01 2011-10-01 false Annual report. 1007.17 Section 1007.17 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OIG AUTHORITIES... abuse and neglect of patients in health care facilities; the number of such complaints investigated...

  12. 42 CFR 1007.17 - Annual report.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 5 2014-10-01 2014-10-01 false Annual report. 1007.17 Section 1007.17 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OIG AUTHORITIES... abuse and neglect of patients in health care facilities; the number of such complaints investigated...

  13. 42 CFR 1007.17 - Annual report.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 5 2012-10-01 2012-10-01 false Annual report. 1007.17 Section 1007.17 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OIG AUTHORITIES... abuse and neglect of patients in health care facilities; the number of such complaints investigated...

  14. ACCJC 1996-1997 Annual Report.

    ERIC Educational Resources Information Center

    Accrediting Commission for Community and Junior Colleges, Santa Rosa, CA.

    The 1996-97 Annual Report from the Accrediting Commission for Community and Junior Colleges (ACCJC) provides public disclosure of the Commission's procedures and policies to help renew public confidence in the importance and effectiveness of peer review and self-regulation in accreditation. The report begins with messages from the Commission's…

  15. Petroleum supply annual 1993. Volume 2

    SciTech Connect

    Not Available

    1994-06-01

    This publication contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1993 through annual and monthly surveys. This second volume contains final statistics for each month of 1993.

  16. Exploring the Genes of Yerba Mate (Ilex paraguariensis A. St.-Hil.) by NGS and De Novo Transcriptome Assembly

    PubMed Central

    Aguilera, Patricia M.; Bubillo, Rosana E.; Otegui, Mónica B.; Ducasse, Daniel A.; Zapata, Pedro D.; Marti, Dardo A.

    2014-01-01

    Yerba mate (Ilex paraguariensis A. St.-Hil.) is an important subtropical tree crop cultivated on 326,000 ha in Argentina, Brazil and Paraguay, with a total yield production of more than 1,000,000 t. Yerba mate presents a strong limitation regarding sequence information. The NCBI GenBank lacks an EST database of yerba mate and depicts only 80 DNA sequences, mostly uncharacterized. In this scenario, in order to elucidate the yerba mate gene landscape by means of NGS, we explored and discovered a vast collection of I. paraguariensis transcripts. Total RNA from I. paraguariensis was sequenced by Illumina HiSeq-2000 obtaining 72,031,388 pair-end 100 bp sequences. High quality reads were de novo assembled into 44,907 transcripts encompassing 40 million bases with an estimated coverage of 180X. Multiple sequence analysis allowed us to predict that yerba mate contains ∼32,355 genes and 12,551 gene variants or isoforms. We identified and categorized members of more than 100 metabolic pathways. Overall, we have identified ∼1,000 putative transcription factors, genes involved in heat and oxidative stress, pathogen response, as well as disease resistance and hormone response. We have also identified, based in sequence homology searches, novel transcripts related to osmotic, drought, salinity and cold stress, senescence and early flowering. We have also pinpointed several members of the gene silencing pathway, and characterized the silencing effector Argonaute1. We predicted a diverse supply of putative microRNA precursors involved in developmental processes. We present here the first draft of the transcribed genomes of the yerba mate chloroplast and mitochondrion. The putative sequence and predicted structure of the caffeine synthase of yerba mate is presented. Moreover, we provide a collection of over 10,800 SSR accessible to the scientific community interested in yerba mate genetic improvement. This contribution broadly expands the limited knowledge of yerba mate genes

  17. Unusual multisystemic involvement and a novel BAG3 mutation revealed by NGS screening in a large cohort of myofibrillar myopathies

    PubMed Central

    2014-01-01

    Background Myofibrillar myopathies (MFM) are a group of phenotypically and genetically heterogeneous neuromuscular disorders, which are characterized by protein aggregations in muscle fibres and can be associated with multisystemic involvement. Methods We screened a large cohort of 38 index patients with MFM for mutations in the nine thus far known causative genes using Sanger and next generation sequencing (NGS). We studied the clinical and histopathological characteristics in 38 index patients and five additional relatives (n = 43) and particularly focused on the associated multisystemic symptoms. Results We identified 14 heterozygous mutations (diagnostic yield of 37%), among them the novel p.Pro209Gln mutation in the BAG3 gene, which was associated with onset in adulthood, a mild phenotype and an axonal sensorimotor polyneuropathy, in the absence of giant axons at the nerve biopsy. We revealed several novel clinical phenotypes and unusual multisystemic presentations with previously described mutations: hearing impairment with a FLNC mutation, dysphonia with a mutation in DES and the first patient with a FLNC mutation presenting respiratory insufficiency as the initial symptom. Moreover, we described for the first time respiratory insufficiency occurring in a patient with the p.Gly154Ser mutation in CRYAB. Interestingly, we detected a polyneuropathy in 28% of the MFM patients, including a BAG3 and a MYOT case, and hearing impairment in 13%, including one patient with a FLNC mutation and two with mutations in the DES gene. In four index patients with a mutation in one of the MFM genes, typical histological findings were only identified at the ultrastructural level (29%). Conclusions We conclude that extraskeletal symptoms frequently occur in MFM, particularly cardiac and respiratory involvement, polyneuropathy and/or deafness. BAG3 mutations should be considered even in cases with a mild phenotype or an adult onset. We identified a genetic defect in one of

  18. Keeping Public Information Public.

    ERIC Educational Resources Information Center

    Kelley, Wayne P.

    1998-01-01

    Discusses the trend toward the transfer of federal government information from the public domain to the private sector. Topics include free access, privatization, information-policy revision, accountability, copyright issues, costs, pricing, and market needs versus public needs. (LRW)

  19. Impact of NGS in the medical sciences: Genetic syndromes with an increased risk of developing cancer as an example of the use of new technologies.

    PubMed

    Lapunzina, Pablo; López, Rocío Ortiz; Rodríguez-Laguna, Lara; García-Miguel, Purificación; Martínez, Augusto Rojas; Martínez-Glez, Víctor

    2014-03-01

    The increased speed and decreasing cost of sequencing, along with an understanding of the clinical relevance of emerging information for patient management, has led to an explosion of potential applications in healthcare. Currently, SNP arrays and Next-Generation Sequencing (NGS) technologies are relatively new techniques used to scan genomes for gains and losses, losses of heterozygosity (LOH), SNPs, and indel variants as well as to perform complete sequencing of a panel of candidate genes, the entire exome (whole exome sequencing) or even the whole genome. As a result, these new high-throughput technologies have facilitated progress in the understanding and diagnosis of genetic syndromes and cancers, two disorders traditionally considered to be separate diseases but that can share causal genetic alterations in a group of developmental disorders associated with congenital malformations and cancer risk. The purpose of this work is to review these syndromes as an example of a group of disorders that has been included in a panel of genes for NGS analysis. We also highlight the relationship between development and cancer and underline the connections between these syndromes. PMID:24764758

  20. Impact of NGS in the medical sciences: Genetic syndromes with an increased risk of developing cancer as an example of the use of new technologies

    PubMed Central

    Lapunzina, Pablo; López, Rocío Ortiz; Rodríguez-Laguna, Lara; García-Miguel, Purificación; Martínez, Augusto Rojas; Martínez-Glez, Víctor

    2014-01-01

    The increased speed and decreasing cost of sequencing, along with an understanding of the clinical relevance of emerging information for patient management, has led to an explosion of potential applications in healthcare. Currently, SNP arrays and Next-Generation Sequencing (NGS) technologies are relatively new techniques used to scan genomes for gains and losses, losses of heterozygosity (LOH), SNPs, and indel variants as well as to perform complete sequencing of a panel of candidate genes, the entire exome (whole exome sequencing) or even the whole genome. As a result, these new high-throughput technologies have facilitated progress in the understanding and diagnosis of genetic syndromes and cancers, two disorders traditionally considered to be separate diseases but that can share causal genetic alterations in a group of developmental disorders associated with congenital malformations and cancer risk. The purpose of this work is to review these syndromes as an example of a group of disorders that has been included in a panel of genes for NGS analysis. We also highlight the relationship between development and cancer and underline the connections between these syndromes. PMID:24764758

  1. Next-generation sequencing (NGS) as a fast molecular diagnosis tool for left ventricular noncompaction in an infant with compound mutations in the MYBPC3 gene.

    PubMed

    Schaefer, Elise; Helms, Pauline; Marcellin, Luc; Desprez, Philippe; Billaud, Philippe; Chanavat, Valérie; Rousson, Robert; Millat, Gilles

    2014-03-01

    Left ventricular noncompaction (LVNC) is a clinically heterogeneous disorder characterized by a trabecular meshwork and deep intertrabecular myocardial recesses that communicate with the left ventricular cavity. LVNC is classified as a rare genetic cardiomyopathy. Molecular diagnosis is a challenge for the medical community as the condition shares morphologic features of hypertrophic and dilated cardiomyopathies. Several genetic causes of LVNC have been reported, with variable modes of inheritance, including autosomal dominant and X-linked inheritance, but relatively few responsible genes have been identified. In this report, we describe a case of a severe form of LVNC leading to death at 6 months of life. NGS sequencing using a custom design for hypertrophic cardiomyopathy panel allowed us to identify compound heterozygosity in the MYBPC3 gene (p.Lys505del, p.Pro955fs) in 3 days, confirming NGS sequencing as a fast molecular diagnosis tool. Other studies have reported neonatal presentation of cardiomyopathies associated with compound heterozygous or homozygous MYBPC3 mutations. In this family and in families in which parental truncating MYBPC3 mutations are identified, preimplantation or prenatal genetic screening should be considered as these genotypes leads to neonatal mortality and morbidity. PMID:24602869

  2. Trends in IT Innovation to Build a Next Generation Bioinformatics Solution to Manage and Analyse Biological Big Data Produced by NGS Technologies

    PubMed Central

    de Brevern, Alexandre G.; Meyniel, Jean-Philippe; Fairhead, Cécile; Neuvéglise, Cécile; Malpertuy, Alain

    2015-01-01

    Sequencing the human genome began in 1994, and 10 years of work were necessary in order to provide a nearly complete sequence. Nowadays, NGS technologies allow sequencing of a whole human genome in a few days. This deluge of data challenges scientists in many ways, as they are faced with data management issues and analysis and visualization drawbacks due to the limitations of current bioinformatics tools. In this paper, we describe how the NGS Big Data revolution changes the way of managing and analysing data. We present how biologists are confronted with abundance of methods, tools, and data formats. To overcome these problems, focus on Big Data Information Technology innovations from web and business intelligence. We underline the interest of NoSQL databases, which are much more efficient than relational databases. Since Big Data leads to the loss of interactivity with data during analysis due to high processing time, we describe solutions from the Business Intelligence that allow one to regain interactivity whatever the volume of data is. We illustrate this point with a focus on the Amadea platform. Finally, we discuss visualization challenges posed by Big Data and present the latest innovations with JavaScript graphic libraries. PMID:26125026

  3. Trends in IT Innovation to Build a Next Generation Bioinformatics Solution to Manage and Analyse Biological Big Data Produced by NGS Technologies.

    PubMed

    de Brevern, Alexandre G; Meyniel, Jean-Philippe; Fairhead, Cécile; Neuvéglise, Cécile; Malpertuy, Alain

    2015-01-01

    Sequencing the human genome began in 1994, and 10 years of work were necessary in order to provide a nearly complete sequence. Nowadays, NGS technologies allow sequencing of a whole human genome in a few days. This deluge of data challenges scientists in many ways, as they are faced with data management issues and analysis and visualization drawbacks due to the limitations of current bioinformatics tools. In this paper, we describe how the NGS Big Data revolution changes the way of managing and analysing data. We present how biologists are confronted with abundance of methods, tools, and data formats. To overcome these problems, focus on Big Data Information Technology innovations from web and business intelligence. We underline the interest of NoSQL databases, which are much more efficient than relational databases. Since Big Data leads to the loss of interactivity with data during analysis due to high processing time, we describe solutions from the Business Intelligence that allow one to regain interactivity whatever the volume of data is. We illustrate this point with a focus on the Amadea platform. Finally, we discuss visualization challenges posed by Big Data and present the latest innovations with JavaScript graphic libraries. PMID:26125026

  4. LLNL NESHAPs 1995 annual report

    SciTech Connect

    Gallegos, G.M.; Harrach, R.J.; Biermann, A.H.; Tate, P.J.

    1996-06-01

    This annual report is prepared pursuant to the National Emissions Standards for Hazardous Air Pollutants (NESHAPs) 40 CFR Part 61, Subpart H; Subpart H governs radionuclide emissions to air from Department of Energy (DOE) facilities. NESHAPs limits the emission of radionuclides to the ambient air from DOE facilities to levels resulting in an effective dose equivalent (EDE) of 10 mrem to any member of the public. This document contains the EDEs for the Lawrence Livermore National Laboratory (LLNL) site-wide maximally exposed members of the public from 1995 operations.

  5. Uranium Industry Annual, 1992

    SciTech Connect

    Not Available

    1993-10-28

    The Uranium Industry Annual provides current statistical data on the US uranium industry for the Congress, Federal and State agencies, the uranium and electric utility industries, and the public. The feature article, ``Decommissioning of US Conventional Uranium Production Centers,`` is included. Data on uranium raw materials activities including exploration activities and expenditures, resources and reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities including domestic uranium purchases, commitments by utilities, procurement arrangements, uranium imports under purchase contracts and exports, deliveries to enrichment suppliers, inventories, secondary market activities, utility market requirements, and uranium for sale by domestic suppliers are presented in Chapter 2.

  6. Uranium industry annual 1996

    SciTech Connect

    1997-04-01

    The Uranium Industry Annual 1996 (UIA 1996) provides current statistical data on the US uranium industry`s activities relating to uranium raw materials and uranium marketing. The UIA 1996 is prepared for use by the Congress, Federal and State agencies, the uranium and nuclear electric utility industries, and the public. Data on uranium raw materials activities for 1987 through 1996 including exploration activities and expenditures, EIA-estimated reserves, mine production of uranium, production of uranium concentrate, and industry employment are presented in Chapter 1. Data on uranium marketing activities for 1994 through 2006, including purchases of uranium and enrichment services, enrichment feed deliveries, uranium fuel assemblies, filled and unfilled market requirements, uranium imports and exports, and uranium inventories are shown in Chapter 2. A feature article, The Role of Thorium in Nuclear Energy, is included. 24 figs., 56 tabs.

  7. Publication planning 101.

    PubMed

    Sismondo, Sergio; Nicholson, Scott Howard

    2009-01-01

    Publication planning is the sub-industry to the pharmaceutical industry that does the organizational and practical work of shaping pharmaceutical companies' data and turning it into medical journal articles. Its main purpose is to create and communicate scientific information to support the marketing of products. This report is based mostly on information presented at the 2007 annual meeting of the International Society of Medical Planning Professionals, including a workshop entitled "Publication Planning 101/201", attended by one of us. We provide some analysis of the role of publication planning in medical publishing, and its implications for the structuring of medical knowledge. PMID:20067704

  8. Effective Public Relations in Extension.

    ERIC Educational Resources Information Center

    Hogan, Mike

    1994-01-01

    Public relations efforts of the Carroll County (Ohio) extension office included periodic reports to legislators, a toll-free number distributed on refrigerator magnets, annual calendar/report to the public, newspaper supplements, and town meetings. Long-term effects were a 116% increase in funding, used to upgrade staff, programs, and…

  9. Ames Research Center publications, 1977

    NASA Technical Reports Server (NTRS)

    1979-01-01

    This bibliography lists 786 formal NASA publications, journal articles, books, chapters of books, patents, and contractor reports which appeared during 1977 or which were not included in previous annual bibliographies. Citations are arranged by directorate, type of publication, and author. Each NASA report is identified by a technical report and accession number to facilitate ordering. An author index is provided.

  10. Petroleum supply annual 1993. Volume 1

    SciTech Connect

    Not Available

    1994-06-01

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1993 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains four sections: Summary Statistics, Detailed Statistics, Refinery Capacity, and Oxygenate Capacity each with final annual data. The second volume contains final statistics for each month of 1993, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. Below is a description of each section in Volume 1 of the PSA.

  11. Petroleum supply annual 1994. Volume 1

    SciTech Connect

    1995-05-22

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1994 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains four sections: Summary Statistics, Detailed Statistics, Refinery Capacity, and Oxygenate Capacity each with final annual data. The second volume contains final statistics for each month of 1994, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. Below is a description of each section in Volume 1 of the PSA.

  12. Petroleum supply annual 1998: Volume 1

    SciTech Connect

    1999-06-01

    The ``Petroleum Supply Annual`` (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1998 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains three sections: Summary Statistics, Detailed Statistics, and Refinery Statistics; each with final annual data. The second volume contains final statistics for each month of 1998, and replaces data previously published in the PSA. The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. 16 figs., 59 tabs.

  13. Petroleum supply annual, 1997. Volume 1

    SciTech Connect

    1998-06-01

    The Petroleum Supply Annual (PSA) contains information on the supply and disposition of crude oil and petroleum products. The publication reflects data that were collected from the petroleum industry during 1997 through annual and monthly surveys. The PSA is divided into two volumes. This first volume contains three sections: Summary Statistics, Detailed Statistics, and Refinery Statistics; each with final annual data. The second volume contains final statistics for each month of 1997, and replaces data previously published in the Petroleum Supply Monthly (PSM). The tables in Volumes 1 and 2 are similarly numbered to facilitate comparison between them. 16 figs., 48 tabs.

  14. Commissioner's Annual Report to the Education Committees of the Senate and General Assembly on Violence, Vandalism and Substance Abuse in New Jersey Public Schools, July 1, 2007 to June 30, 2008

    ERIC Educational Resources Information Center

    New Jersey Department of Education, 2009

    2009-01-01

    The Commissioner's annual report provides the Legislature with information reported by school districts concerning incidents of serious student misconduct grouped into the following four major reporting categories: violence, vandalism, weapons, and substance abuse. An analysis of trends yields indications of progress and of ongoing concern, and…

  15. Commissioner's Annual Report to the Education Committees of the Senate and General Assembly on Violence, Vandalism and Substance Abuse in New Jersey Public Schools, July 1, 2008 to June 30, 2009

    ERIC Educational Resources Information Center

    New Jersey Department of Education, 2010

    2010-01-01

    The Commissioner's annual report provides the Legislature with information reported by school districts concerning incidents of serious student misconduct grouped into the following four major reporting categories: violence, vandalism, weapons, and substance abuse. An analysis of trends yields indications of progress and of ongoing concern, and…

  16. To Assure the Free Appropriate Public Education of All Children with Disabilities (Individuals with Disabilities Education Act, Section 618). Twenty-Second Annual Report to Congress on the Implementation of the Individuals with Disabilities Education Act. 2000.

    ERIC Educational Resources Information Center

    Department of Education, Washington, DC.

    This 22nd annual report to Congress on the implementation of the Individuals with Disabilities Education Act 1997 (IDEA) begins with a special preface reflecting on the progress made in the 25 years since the initial passage of the law. Following the preface, Section 1 examines contextual and environmental factors such as the role of minority…

  17. Threshold Concepts: From Personal Practice to Communities of Practice. Proceedings of the National Academy's Sixth Annual Conference and the Fourth Biennial Threshold Concepts Conference [E-publication] (Dublin, Ireland, June 27-29, 2012)

    ERIC Educational Resources Information Center

    O'Mahony, Catherine, Ed.; Buchanan, Avril, Ed.; O'Rourke, Mary, Ed.; Higgs, Bettie, Ed.

    2014-01-01

    The 6th Annual Conference of the National Academy for the Integration of Research, Teaching and Learning (NAIRTL) and the 4th Biennial Threshold Concepts Conference was held at Trinity College Dublin, Ireland, on June 27-29, 2012. The NAIRTL is a collaborative initiative between University College Cork, Cork Institute of Technology, National…

  18. Science, Technology, and American Diplomacy 1985. Sixth Annual Report Submitted to the Congress by the President Pursuant to Section 503(b) of Title V of Public Law 95-426. Serial E.

    ERIC Educational Resources Information Center

    Department of State, Washington, DC.

    The Reagan administration's annual report to the Congress on international activities in the fields of science and technology (S&T) for fiscal year 1984 consists of three parts. The two chapters in part I (S&T in American diplomacy) examine S&T in American foreign policy and resources necessary for successful diplomacy. The two chapters in part II…

  19. Applicability and Efficiency of NGS in Routine Diagnosis: In-Depth Performance Analysis of a Complete Workflow for CFTR Mutation Analysis

    PubMed Central

    Pagin, Adrien; Devos, Aurore; Figeac, Martin; Truant, Maryse; Willoquaux, Christelle; Broly, Franck; Lalau, Guy

    2016-01-01

    Background Actually, about 2000 sequence variations have been documented in the CFTR gene requiring extensive and multi-step genetic testing in the diagnosis of cystic fibrosis and CFTR-related disorders. We present a two phases study, with validation and performance monitoring, of a single experiment methodology based on multiplex PCR and high throughput sequencing that allows detection of all variants, including large rearrangements, affecting the coding regions plus three deep intronic loci. Methods A total of 340 samples, including 257 patients and 83 previously characterized control samples, were sequenced in 17 MiSeq runs and analyzed with two bioinformatic pipelines in routine diagnostic conditions. We obtained 100% coverage for all the target regions in every tested sample. Results We correctly identified all the 87 known variants in the control samples and successfully confirmed the 62 variants identified among the patients without observing false positive results. Large rearrangements were identified in 18/18 control samples. Only 17 patient samples showed false positive signals (6.6%), 12 of which showed a borderline result for a single amplicon. We also demonstrated the ability of the assay to detect allele specific dropout of amplicons when a sequence variation occurs at a primer binding site thus limiting the risk for false negative results. Conclusions We described here the first NGS workflow for CFTR routine analysis that demonstrated equivalent diagnostic performances compared to Sanger sequencing and multiplex ligation-dependent probe amplification. This study illustrates the advantages of NGS in term of scalability, workload reduction and cost-effectiveness in combination with an improvement of the overall data quality due to the simultaneous detection of SNVs and large rearrangements. PMID:26900683

  20. Apprentices and Trainees: Annual, 2009. Terms and Definitions. Support Document

    ERIC Educational Resources Information Center

    National Centre for Vocational Education Research (NCVER), 2010

    2010-01-01

    This document covers the data terms used in the "Apprentices and trainees: Annual 2009" publication and its associated data tables. The primary purpose of this document is to assist users of the publication to understand the specific data terms used within it. Terms that appear in the publication are listed in alphabetical order with the following…

  1. 45 CFR 1168.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Semi-annual compilation. 1168.600 Section 1168.600 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Agency Reports § 1168.600...

  2. 45 CFR 1168.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Semi-annual compilation. 1168.600 Section 1168.600 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Agency Reports § 1168.600...

  3. 45 CFR 1168.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Semi-annual compilation. 1168.600 Section 1168.600 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Agency Reports § 1168.600...

  4. 45 CFR 1168.600 - Semi-annual compilation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Semi-annual compilation. 1168.600 Section 1168.600 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Agency Reports § 1168.600...

  5. 45 CFR 1168.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Semi-annual compilation. 1168.600 Section 1168.600 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Agency Reports § 1168.600...

  6. 42 CFR 422.306 - Annual MA capitation rates.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 3 2011-10-01 2011-10-01 false Annual MA capitation rates. 422.306 Section 422.306....306 Annual MA capitation rates. Subject to adjustments at §§ 422.308(b) and 422.308(g), the annual capitation rate for each MA local area is determined under paragraph (a) of this section for 2005 and...

  7. 42 CFR 422.306 - Annual MA capitation rates.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 3 2013-10-01 2013-10-01 false Annual MA capitation rates. 422.306 Section 422.306... Organizations § 422.306 Annual MA capitation rates. Subject to adjustments at §§ 422.308(b) and 422.308(g), the annual capitation rate for each MA local area is determined under paragraph (a) of this section for...

  8. 42 CFR 422.306 - Annual MA capitation rates.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 3 2012-10-01 2012-10-01 false Annual MA capitation rates. 422.306 Section 422.306... Organizations § 422.306 Annual MA capitation rates. Subject to adjustments at §§ 422.308(b) and 422.308(g), the annual capitation rate for each MA local area is determined under paragraph (a) of this section for...

  9. 42 CFR 422.306 - Annual MA capitation rates.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 3 2014-10-01 2014-10-01 false Annual MA capitation rates. 422.306 Section 422.306... Organizations § 422.306 Annual MA capitation rates. Subject to adjustments at §§ 422.308(b) and 422.308(g), the annual capitation rate for each MA local area is determined under paragraph (a) of this section for...

  10. 15 CFR 200.106 - Publications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... NIST programs and services to its varied technical audiences, as well as to the general public. NIST... Standards & Technology” 1 and SP 260 “NIST Standard Reference Materials Catalog.” 1 A complete catalog of all publications by NIST authors is issued annually as a supplement to SP 305 “Publications of...

  11. 15 CFR 200.106 - Publications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... NIST programs and services to its varied technical audiences, as well as to the general public. NIST... Standards & Technology” 1 and SP 260 “NIST Standard Reference Materials Catalog.” 1 A complete catalog of all publications by NIST authors is issued annually as a supplement to SP 305 “Publications of...

  12. Maryland's Achievements in Public Education, 2011

    ERIC Educational Resources Information Center

    Maryland State Department of Education, 2011

    2011-01-01

    This report presents Maryland's achievements in public education for 2011. Maryland's achievements include: (1) Maryland's public schools again ranked #1 in the nation in Education Week's 2011 Quality Counts annual report; (2) Maryland ranked 1st nationwide for a 3rd year in a row in the percentage of public school students scoring 3 or higher on…

  13. 1 CFR 10.10 - Publication required.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 1 General Provisions 1 2014-01-01 2012-01-01 true Publication required. 10.10 Section 10.10 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER PRESIDENTIAL PAPERS Annual Publication § 10.10 Publication required. The Director of the...

  14. 1 CFR 10.10 - Publication required.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 1 General Provisions 1 2013-01-01 2012-01-01 true Publication required. 10.10 Section 10.10 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER PRESIDENTIAL PAPERS Annual Publication § 10.10 Publication required. The Director of the...

  15. 1 CFR 10.10 - Publication required.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 1 General Provisions 1 2011-01-01 2011-01-01 false Publication required. 10.10 Section 10.10 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER PRESIDENTIAL PAPERS Annual Publication § 10.10 Publication required. The Director of the...

  16. 1 CFR 10.10 - Publication required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 1 General Provisions 1 2010-01-01 2010-01-01 false Publication required. 10.10 Section 10.10 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER PRESIDENTIAL PAPERS Annual Publication § 10.10 Publication required. The Director of the...

  17. 1 CFR 10.10 - Publication required.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 1 General Provisions 1 2012-01-01 2012-01-01 false Publication required. 10.10 Section 10.10 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER PRESIDENTIAL PAPERS Annual Publication § 10.10 Publication required. The Director of the...

  18. 78 FR 74048 - Eleventh Coast Guard District Annual Fireworks Events

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ...The Coast Guard proposes to amend several permanent safety zones located in the Eleventh Coast Guard District that are established to protect public safety during annual firework displays. These amendments will standardize the safety zone language, update listed events, delete events that are no longer occurring, add new annual fireworks events, and establish a standardized format using a......

  19. 45 CFR 1801.1 - Annual Truman Scholarship competition.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Annual Truman Scholarship competition. 1801.1... SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM General § 1801.1 Annual Truman Scholarship competition. Each year, the Harry S. Truman Scholarship Foundation carries out a nationwide competition...

  20. 45 CFR 1801.1 - Annual Truman Scholarship competition.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Annual Truman Scholarship competition. 1801.1... SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM General § 1801.1 Annual Truman Scholarship competition. Each year, the Harry S. Truman Scholarship Foundation carries out a nationwide competition...

  1. 45 CFR 1801.1 - Annual Truman Scholarship competition.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Annual Truman Scholarship competition. 1801.1... SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM General § 1801.1 Annual Truman Scholarship competition. Each year, the Harry S. Truman Scholarship Foundation carries out a nationwide competition...

  2. 45 CFR 1801.1 - Annual Truman Scholarship competition.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Annual Truman Scholarship competition. 1801.1... SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM General § 1801.1 Annual Truman Scholarship competition. Each year, the Harry S. Truman Scholarship Foundation carries out a nationwide competition...

  3. 45 CFR 1801.1 - Annual Truman Scholarship competition.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Annual Truman Scholarship competition. 1801.1... SCHOLARSHIP FOUNDATION HARRY S. TRUMAN SCHOLARSHIP PROGRAM General § 1801.1 Annual Truman Scholarship competition. Each year, the Harry S. Truman Scholarship Foundation carries out a nationwide competition...

  4. The Annual Report of the ACTFL Executive Director.

    ERIC Educational Resources Information Center

    Scebold, C. Edward

    1979-01-01

    Presents an overview of the President's Commission on Foreign Language and International Studies and the MLA/ACLS Task Force reports. The annual report of the American Council on the Teaching of Foreign Language includes: its organization, publications; annual meeting and preconference workshops, and projects. (EJS)

  5. 15 CFR 28.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Semi-annual compilation. 28.600... Agency Reports § 28.600 Semi-annual compilation. (a) The head of each agency shall collect and compile... committees. Such information shall not be available for public inspection. (e) The first...

  6. 14 CFR 1271.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Semi-annual compilation. 1271.600 Section... LOBBYING Agency Reports § 1271.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...

  7. 31 CFR 21.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Semi-annual compilation. 21.600... LOBBYING Agency Reports § 21.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...

  8. 28 CFR 69.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Semi-annual compilation. 69.600 Section... Reports § 69.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the... committees. Such information shall not be available for public inspection. (e) The first...

  9. 28 CFR 69.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Semi-annual compilation. 69.600 Section... Reports § 69.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the... committees. Such information shall not be available for public inspection. (e) The first...

  10. 14 CFR 1271.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Semi-annual compilation. 1271.600 Section... LOBBYING Agency Reports § 1271.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...

  11. 31 CFR 21.600 - Semi-annual compilation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Semi-annual compilation. 21.600... LOBBYING Agency Reports § 21.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...

  12. 12 CFR 411.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 4 2011-01-01 2011-01-01 false Semi-annual compilation. 411.600 Section 411... Reports § 411.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the... committees. Such information shall not be available for public inspection. (e) The first...

  13. 31 CFR 21.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Semi-annual compilation. 21.600... LOBBYING Agency Reports § 21.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...

  14. 28 CFR 69.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Semi-annual compilation. 69.600 Section... Reports § 69.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the... committees. Such information shall not be available for public inspection. (e) The first...

  15. 31 CFR 21.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Semi-annual compilation. 21.600... LOBBYING Agency Reports § 21.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...

  16. 15 CFR 28.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Semi-annual compilation. 28.600... Agency Reports § 28.600 Semi-annual compilation. (a) The head of each agency shall collect and compile... committees. Such information shall not be available for public inspection. (e) The first...

  17. 15 CFR 28.600 - Semi-annual compilation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Semi-annual compilation. 28.600... Agency Reports § 28.600 Semi-annual compilation. (a) The head of each agency shall collect and compile... committees. Such information shall not be available for public inspection. (e) The first...

  18. 14 CFR 1271.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Semi-annual compilation. 1271.600 Section... LOBBYING Agency Reports § 1271.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...

  19. 22 CFR 712.600 - Semi-annual compilation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Semi-annual compilation. 712.600 Section 712.600... LOBBYING Agency Reports § 712.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...

  20. 22 CFR 712.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Semi-annual compilation. 712.600 Section 712.600... LOBBYING Agency Reports § 712.600 Semi-annual compilation. (a) The head of each agency shall collect and... committees. Such information shall not be available for public inspection. (e) The first...