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Sample records for asynchronous quantal release

  1. Direct measurement of ACh release from exposed frog nerve terminals: constraints on interpretation of non-quantal release.

    PubMed Central

    Grinnell, A D; Gundersen, C B; Meriney, S D; Young, S H

    1989-01-01

    1. Acetylcholine (ACh) release from enzymatically exposed frog motor nerve terminals has been measured directly with closely apposed outside-out clamped patches of Xenopus myocyte membrane, rich in ACh receptor channels. When placed close to the synaptic surface of the terminal, such a membrane patch detects both nerve-evoked patch currents (EPCs) and spontaneous quantal 'miniature' patch currents (MPCs), from a few micrometres length of the terminal, in response to ACh release from the nearest three to five active zones. 2. Chemical measurements of ACh efflux from whole preparations revealed a spontaneous release rate of 4.1 pmol (2 h)-1, and no significant difference in resting efflux between enzyme-treated and control preparations. The ratio of enzyme-treated to contralateral control muscle efflux averaged 1.17, indicating that enzyme treatment did not affect spontaneous ACh release. Vesamicol (1.7 microM), which blocks the ACh transporter in synaptic vesicles, decreased the spontaneous release of ACh to 67% of control. 3. In the absence of nerve stimulation, the frequency of single-channel openings recorded by outside-out patch probes adjacent to nerve terminals was very low (1-2 min-1), and little different at a distance of hundreds of micrometres, suggesting that if ACh was continually leaking from the terminal in a non-quantal fashion, the amount being released near active zone regions on the terminal was below the limit of detection with the patches. 4. Direct measurements of the sensitivity of the patches, coupled with calculated ACh flux rates, lead to the conclusion that the amount of ACh released non-quantally from the synaptic surface of the frog nerve terminal is less than one-tenth the amount expected if all non-quantal release is from this region of the terminal membrane. 5. Following a series of single nerve shocks or a 50 Hz train of nerve stimuli, the frequency of asynchronous single-channel openings increased for several seconds. This transient

  2. Slow kinetics of inositol 1,4,5-trisphosphate-induced Ca2+ release: is the release 'quantal' or 'non-quantal'?

    PubMed Central

    Missiaen, L; De Smedt, H; Parys, J B; Sienaert, I; Sipma, H; Vanlingen, S; Casteels, R

    1997-01-01

    Inositol 1,4,5-trisphosphate (InsP3)-induced Ca2+ release from intracellular stores is generally assumed to be a 'quantal' process because low InsP3 concentrations mobilize less Ca2+ than high concentrations and a submaximal concentration does not release all the InsP3-mobilizable Ca2+. However, some recent reports questioned the generally accepted view that a low dose of InsP3 is unable to empty the whole store. We have now challenged the stores of permeabilized A7r5 cells in InsP3 for much longer periods than previously reported, to assess directly whether the slow phase of the release would empty the whole store (a non-quantal response) or only a fraction of it (a quantal response). Addition of a maximal [InsP3] at the end of a prolonged (92 min) stimulation with a submaximal [InsP3] resulted in additional Ca2+ release. Experiments in which the stores were challenged with different submaximal InsP3 concentrations for long time periods revealed that a lower [InsP3] never released the same amount of Ca2+ as a higher [InsP3]. This quantal pattern of Ca2+ release occurred both at 25 degrees C and at 4 degrees C. There was a time-dependent increase in the fraction of Ca2+ recruited by the lower compared with the higher [InsP3]. This recruitment of Ca2+ persisted if the [InsP3] was decreased, but was largely prevented by palmitoyl-CoA, a potent blocker of the luminal Ca2+ translocation between individual store units. ATP, in the presence of InsP3, released Ca2+ under conditions permitting the recruitment of no additional InsP3 receptors, indicating that an all-or-none emptying of a fraction of the stores cannot be the only mechanism responsible for quantal Ca2+ release in A7r5 cells. We conclude that some of the previously published evidence for a non-quantal Ca2+ release pattern should be reinterpreted. PMID:9173870

  3. Electroporation followed by electrochemical measurement of quantal transmitter release from single cells using a patterned microelectrode

    PubMed Central

    Ghosh, Jaya; Liu, Xin; Gillis, Kevin D.

    2013-01-01

    An electrochemical microelectrode located immediately adjacent to a single neuroendocrine cell can record spikes of amperometric current that result from exocytosis of oxidizable transmitter from individual vesicles, i.e., quantal exocytosis. Here, we report the development of an efficient method where the same electrochemical microelectrode is used to electropermeabilize an adjacent chromaffin cell and then measure the consequent quantal catecholamine release using amperometry. Trains of voltage pulses, 5–7 V in amplitude and 0.1–0.2 ms in duration, were used to reliably trigger release from cells using gold electrodes. Amperometric spikes induced by electropermeabilization had similar areas, peak heights and durations as amperometric spikes elicited by depolarizing high K+ solutions, therefore release occurs from individual secretory granules. Uptake of trypan blue stain into cells demonstrated that the plasma membrane is permeabilized by the voltage stimulus. Voltage pulses did not degrade the electrochemical sensitivity of the electrodes assayed using a test analyte. Surprisingly, robust quantal release was elicited upon electroporation in the absence of Ca2+ in the bath solution (0 Ca2+/5 mM EGTA). In contrast, electropermeabilization-induced transmitter release required Cl− in the bath solution in that bracketed experiments demonstrated a steep dependence of the rate of electropermeabilization-induced transmitter release on [Cl−] between 2 and 32 mM. Using the same electrochemical electrode to electroporate and record quantal release of catecholamines from an individual chromaffin cell allows precise timing of the stimulus, stimulation of a single cell at a time, and can be used to load membrane-impermeant substances into a cell. PMID:23598689

  4. A Phenomenological Synapse Model for Asynchronous Neurotransmitter Release

    PubMed Central

    Wang, Tao; Yin, Luping; Zou, Xiaolong; Shu, Yousheng; Rasch, Malte J.; Wu, Si

    2016-01-01

    Neurons communicate with each other via synapses. Action potentials cause release of neurotransmitters at the axon terminal. Typically, this neurotransmitter release is tightly time-locked to the arrival of an action potential and is thus called synchronous release. However, neurotransmitter release is stochastic and the rate of release of small quanta of neurotransmitters can be considerably elevated even long after the ceasing of spiking activity, leading to asynchronous release of neurotransmitters. Such asynchronous release varies for tissue and neuron types and has been shown recently to be pronounced in fast-spiking neurons. Notably, it was found that asynchronous release is enhanced in human epileptic tissue implicating a possibly important role in generating abnormal neural activity. Current neural network models for simulating and studying neural activity virtually only consider synchronous release and ignore asynchronous transmitter release. Here, we develop a phenomenological model for asynchronous neurotransmitter release, which, on one hand, captures the fundamental features of the asynchronous release process, and, on the other hand, is simple enough to be incorporated in large-size network simulations. Our proposed model is based on the well-known equations for short-term dynamical synaptic interactions and includes an additional stochastic term for modeling asynchronous release. We use experimental data obtained from inhibitory fast-spiking synapses of human epileptic tissue to fit the model parameters, and demonstrate that our model reproduces the characteristics of realistic asynchronous transmitter release. PMID:26834617

  5. Effect of hypertonicity on augmentation and potentiation and on corresponding quantal parameters of transmitter release.

    PubMed

    Cheng, H; Miyamoto, M D

    1999-03-01

    Augmentation and (posttetanic) potentiation are two of the four components comprising the enhanced release of transmitter following repetitive nerve stimulation. To examine the quantal basis of these components under isotonic and hypertonic conditions, we recorded miniature endplate potentials (MEPPs) from isolated frog (Rana pipiens) cutaneous pectoris muscles, before and after repetitive nerve stimulation (40 s at 80 Hz). Continuous recordings were made in low Ca2+ high Mg2+ isotonic Ringer solution, in Ringer that was made hypertonic with 100 mM sucrose, and in wash solution. Estimates were obtained of m (no. of quanta released), n (no. of functional release sites), p (mean probability of release), and vars p (spatial variance in p), using a method that employed MEPP counts. Hypertonicity abolished augmentation without affecting potentiation. There were prolonged poststimulation increases in m, n, and p and a marked but transient increase in vars p in the hypertonic solution. All effects were completely reversed with wash. The time constants of decay for potentiation and for vars p were virtually identical. The results are consistent with the notion that augmentation is caused by Ca2+ influx through voltage-gated calcium channels and that potentiation is due to Na+-induced Ca2+ release from mitochondria. The results also demonstrate the utility of this approach for analyzing the dynamics of quantal transmitter release. PMID:10085369

  6. Effect of N-acetylaspartylglutamate (NAAG) on non-quantal and spontaneous quantal release of acetylcholine at the neuromuscular synapse of rat.

    PubMed

    Malomouzh, Artem I; Nikolsky, Eugen E; Lieberman, Edward M; Sherman, Jessica A; Lubischer, Jane L; Grossfeld, Robert M; Urazaev, Albert Kh

    2005-07-01

    N-Acetylaspartylglutamate (NAAG), known to be present in rat motor neurons, may participate in neuronal modulation of non-quantal secretion of acetylcholine (ACh) from motor nerve terminals. Non-quantal release of ACh was estimated by the amplitude of the endplate membrane hyperpolarization (H-effect) caused by inhibition of nicotinic receptors by (+)-tubocurarine and acetylcholinesterase by armin (diethoxy-p-nitrophenyl phosphate). Application of exogenous NAAG decreased the H-effect in a dose-dependent manner. The reduction of the H-effect by NAAG was completely removed when N-acetyl-beta-aspartylglutamate (betaNAAG) or 2-(phosphonomethyl)-pentanedioic acid (2-PMPA) was used to inhibit glutamate carboxypeptidase II (GCP II), a presynaptic Schwann cell membrane-associated ectoenzyme that hydrolyzes NAAG to glutamate and N-acetylaspartate. Bath application of glutamate decreased the H-effect similarly to the action of NAAG but N-acetylaspartate was without effect. Inhibition of NMDA receptors by dl-2-amino-5-phosphopentanoic acid, (+)-5-methyl-10,11-dihydro-5H-dibenzocyclohepten-5,10-imine (MK801), and 7-chlorokynurenic acid or inhibition of muscle nitric oxide synthase (NO synthase) by N(G)-nitro-l-arginine methyl ester and 3-bromo-7-nitroindazole completely prevented the decrease of the H-effect by NAAG. These results suggest that glutamate, produced by enzymatic hydrolysis of bath-applied NAAG, can modulate non-quantal secretion of ACh from the presynaptic terminal of the neuromuscular synapse via activation of postsynaptic NMDA receptors and synthesis of nitric oxide (NO) in muscle fibers. NAAG also increased the frequency of miniature endplate potentials (mEPPs) generated by spontaneous quantal secretion of ACh, whereas the mean amplitude and time constants for rise time and for decay of mEPPs did not change. PMID:15953368

  7. Mouse motor nerve terminal immunoreactivity to synaptotagmin II during sustained quantal transmitter release.

    PubMed

    Angaut-Petit, D; Juzans, P; Molgó, J; Faille, L; Seagar, M J; Takahashi, M; Shoji-Kasai, Y

    1995-05-29

    An antibody directed against the lumenal NH2-terminus of synaptotagmin II was used to examine the distribution of this vesicular protein either after spontaneous acetylcholine release or after sustained release induced by La3+ or alpha-latrotoxin, in conditions that prevent endocytosis. The detection of the epitope was examined in the presence or absence of Triton X-100. We show that, in resting conditions of transmitter release, permeabilization of nerve terminal membranes is required for obvious detection of synaptotagmin Ii immunoreactivity whereas during sustained rates of quantal release, permeabilization is not necessary. These data indicate that, in the latter conditions, synaptotagmin II is incorporated into the terminal axolemma and its intravesicular domain exposed at the extracellular nerve terminal surface. PMID:7552284

  8. Molecular mechanisms for synchronous, asynchronous, and spontaneous neurotransmitter release.

    PubMed

    Kaeser, Pascal S; Regehr, Wade G

    2014-01-01

    Most neuronal communication relies upon the synchronous release of neurotransmitters, which occurs through synaptic vesicle exocytosis triggered by action potential invasion of a presynaptic bouton. However, neurotransmitters are also released asynchronously with a longer, variable delay following an action potential or spontaneously in the absence of action potentials. A compelling body of research has identified roles and mechanisms for synchronous release, but asynchronous release and spontaneous release are less well understood. In this review, we analyze how the mechanisms of the three release modes overlap and what molecular pathways underlie asynchronous and spontaneous release. We conclude that the modes of release have key fusion processes in common but may differ in the source of and necessity for Ca(2+) to trigger release and in the identity of the Ca(2+) sensor for release. PMID:24274737

  9. Molecular Mechanisms for Synchronous, Asynchronous, and Spontaneous Neurotransmitter Release

    PubMed Central

    Kaeser, Pascal S.; Regehr, Wade G.

    2015-01-01

    Most neuronal communication relies upon the synchronous release of neurotransmitters, which occurs through synaptic vesicle exocytosis triggered by action potential invasion of a presynaptic bouton. However, neurotransmitters are also released asynchronously with a longer, variable delay following an action potential or spontaneously in the absence of action potentials. A compelling body of research has identified roles and mechanisms for synchronous release, but asynchronous release and spontaneous release are less well understood. In this review, we analyze how the mechanisms of the three release modes overlap and what molecular pathways underlie asynchronous and spontaneous release. We conclude that the modes of release have key fusion processes in common but may differ in the source of and necessity for Ca2+ to trigger release and in the identity of the Ca2+ sensor for release. PMID:24274737

  10. Receptor-mediated presynaptic facilitation of quantal release of acetylcholine induced by pralidoxime in Aplysia.

    PubMed

    Fossier, P; Baux, G; Poulain, B; Tauc, L

    1990-09-01

    1. Possible interactions of contrathion (pralidoxime sulfomethylate), a reactivator of phosphorylated acetylcholinesterase (AChE), with the regulation of cholinergic transmission were investigated on an identified synapse in the buccal ganglion of Aplysia californica. 2. Transmitter release was evoked either by a presynaptic action potential or, under voltage clamp, by a long depolarization of the presynaptic cell. At concentrations higher than 10(-5) M, bath-applied contrathion decreased the amplitude of miniature postsynaptic currents and increased their decay time. At the same time, the quantal release of ACh was transiently facilitated. The facilitatory effect of contrathion was prevented by tubocurarine but not by atropine. Because in this preparation, these drugs block, respectively, the presynaptic nicotinic-like and muscarinic-like receptors involved in positive and negative feedback of ACh release, we proposed that contrathion activates presynaptic nicotinic-like receptors. 3. Differential desensitization of the presynaptic receptors is proposed to explain the transience of the facilitatory action of contrathion on ACh release. 4. The complexity of the synaptic action of contrathion raises the possibility that its therapeutic effects in AChE poisonings are not limited to AChE reactivation. PMID:2253262

  11. Vesicles derived via AP-3-dependent recycling contribute to asynchronous release and influence information transfer.

    PubMed

    Evstratova, Alesya; Chamberland, Simon; Faundez, Victor; Tóth, Katalin

    2014-01-01

    Action potentials trigger synchronous and asynchronous neurotransmitter release. Temporal properties of both types of release could be altered in an activity-dependent manner. While the effects of activity-dependent changes in synchronous release on postsynaptic signal integration have been studied, the contribution of asynchronous release to information transfer during natural stimulus patterns is unknown. Here we find that during trains of stimulations, asynchronous release contributes to the precision of action potential firing. Our data show that this form of release is selectively diminished in AP-3b2 KO animals, which lack functional neuronal AP-3, an adaptor protein regulating vesicle formation from endosomes generated during bulk endocytosis. We find that in the absence of neuronal AP-3, asynchronous release is attenuated and the activity-dependent increase in the precision of action potential timing is compromised. Lack of asynchronous release decreases the capacity of synaptic information transfer and renders synaptic communication less reliable in response to natural stimulus patterns. PMID:25410111

  12. Intestinal and peritoneal mast cells differ in kinetics of quantal release.

    PubMed

    Balseiro-Gomez, Santiago; Ramirez-Ponce, M Pilar; Acosta, Jorge; Ales, Eva; Flores, Juan A

    2016-01-15

    5-hydroxytriptamine (5-HT, serotonin) storage and release in mast cell (MC) secretory granules (SG) are dependent on serglycin proteoglycans (PG). This notion is based on the studies of MC of the connective tissue subtype that predominantly contain PG of the heparin type, whereas intestinal mucosal MC, which contain predominantly chondroitin sulfate, have been poorly explored. In the present study, we addressed the possibility that PG contents may differently affect the storage and release of preformed mediators in these two MC subclasses and explain in part their different functional properties. Rat peritoneal (PMC) and intestinal mast cells (IMC) were isolated and purified using a percoll gradient, and the efflux of 5-HT from each SG was measured by amperometric detection. IMC exhibited a ∼34% reduction in the release of 5-HT compared with PMC because of a lower number of exocytotic events, rather than a lower secretion per single exocytotic event. Amperometric spikes from IMC exhibited a slower decay phase and increased half-width but a similar ascending phase and foot parameters, indicating that the fusion pore kinetics are comparable in both MC subclasses. We conclude that both PG subtypes are equally efficient systems, directly involved in serotonin accumulation, and play a crucial role in regulating the kinetics of exocytosis from SG, providing specific secretory properties for the two cellular subtypes. PMID:26692491

  13. Controlled on-chip stimulation of quantal catecholamine release from chromaffin cells using photolysis of caged Ca2+ on transparent indium-tin-oxide microchip electrodes.

    PubMed

    Chen, Xiaohui; Gao, Yuanfang; Hossain, Maruf; Gangopadhyay, Shubhra; Gillis, Kevin D

    2008-01-01

    Photorelease of caged Ca(2+) is a uniquely powerful tool to study the dynamics of Ca(2+)-triggered exocytosis from individual cells. Using photolithography and other microfabrication techniques, we have developed transparent microchip devices to enable photorelease of caged Ca(2+), together with electrochemical detection of quantal catecholamine secretion from individual cells or cell arrays as a step towards developing high-throughput experimental devices. A 100 nm thick transparent indium-tin-oxide (ITO) film was sputter-deposited onto glass coverslips, which were then patterned into 24 cell-sized working electrodes (approximately 20 microm by 20 microm). We loaded bovine chromaffin cells with acetoxymethyl (AM) ester derivatives of the Ca(2+) cage NP-EGTA and Ca(2+) indicator dye fura-4F, then transferred these cells onto the working ITO electrodes for amperometric recordings. Upon flash photorelease of caged Ca(2+), a uniform rise of [Ca(2+)](i) within the target cell leads to quantal release of oxidizable catecholamines measured amperometrically by the underlying ITO electrode. We observed a burst of amperometric spikes upon rapid elevation of [Ca(2+)](i) and a "priming" effect of sub-stimulatory [Ca(2+)](i) on the response of cells to subsequent [Ca(2+)](i) elevation, similar to previous reports using different techniques. We conclude that UV photolysis of caged Ca(2+) is a suitable stimulation technique for higher-throughput studies of Ca(2+)-dependent exocytosis on transparent electrochemical microelectrode arrays. PMID:18094774

  14. Controlled On-chip Stimulation of Quantal Catecholamine Release from Chromaffin Cells Using Photolysis of Caged Ca2+ on Transparent Indium-Tin-Oxide Microchip Electrodes

    PubMed Central

    Chen, Xiaohui; Gao, Yuanfang; Hossain, Maruf; Gangopadhyay, Shubhra; Gillis, Kevin D.

    2008-01-01

    Photorelease of caged Ca2+ is a uniquely powerful tool to study the dynamics of Ca2+-triggered exocytosis from individual cells. Using photolithography and other microfabrication techniques, we have developed transparent microchip devices to enable photorelease of caged Ca2+ together with electrochemical detection of quantal catecholamine secretion from individual cells or cell arrays as a step towards developing high-throughput experimental devices. A 100 nm - thick transparent Indium-Tin-Oxide (ITO) film was sputter-deposited onto glass coverslips, which were then patterned into 24 cell-sized working electrodes (∼20 μm by 20 μm). We loaded bovine chromaffin cells with acetoxymethyl (AM) ester derivatives of the Ca2+ cage NP-EGTA and Ca2+ indicator dye Fura-4F, then transferred these cells onto the working ITO electrodes for amperometric recordings. Upon flash photorelease of caged Ca2+, a uniform rise of [Ca2+]i within the target cell leads to quantal release of oxidizable catecholamines measured amperometrically by the underlying ITO electrode. We observed a burst of amperometric spikes upon rapid elevation of [Ca2+]i and a “priming” effect of sub-stimulatory [Ca2+]i on the response of cells to subsequent [Ca2+]i elevation, similar to previous reports using different techniques. We conclude that UV photolysis of caged Ca2+ is a suitable stimulation technique for higher-throughput studies of Ca2+-dependent exocytosis on transparent electrochemical microelectrode arrays. PMID:18094774

  15. Illustrating Concepts of Quantal Analysis with an Intuitive Classroom Model

    ERIC Educational Resources Information Center

    Xu-Friedman, Matthew A.

    2013-01-01

    The quantal hypothesis is central to the modern understanding of how a neurotransmitter is released from synapses. This hypothesis expresses that a neurotransmitter is packaged together in quanta that are released probabilistically. The experiments that led to the quantal hypothesis are often related in introductory neuroscience textbooks, but…

  16. Presynaptic Kainate Receptor Activation Preserves Asynchronous GABA Release Despite the Reduction in Synchronous Release from Hippocampal CCK Interneurons

    PubMed Central

    Daw, Michael I.; Pelkey, Kenneth A.; Chittajallu, Ramesh; McBain, Chris J.

    2010-01-01

    Inhibitory synaptic transmission in the hippocampus in mediated by a wide variety of different interneuron classes which are assumed to play different roles in network activity. Activation of presynaptic kainate receptors (KARs) has been shown to reduce inhibitory transmission but the interneuron class(es) at which they act is only recently beginning to emerge. Using paired recordings we show that KAR activation causes a decrease in presynaptic release from CCK- but not PV-containing interneurons and that this decrease is observed when pyramidal cells, but not interneurons, are the postsynaptic target. We also show that although the synchronous release component is reduced, the barrage of asynchronous GABA release from CCK interneurons during sustained firing is unaffected by KAR activation. This indicates that presynaptic KARs preserve and act in concert with asynchronous release to switch CCK interneurons from a phasic inhibition mode to produce prolonged inhibition during periods of intense activity. PMID:20720128

  17. Presynaptic Molecular Determinants of Quantal Size

    PubMed Central

    Takamori, Shigeo

    2016-01-01

    The quantal hypothesis for the release of neurotransmitters at the chemical synapse has gained wide acceptance since it was first worked out at the motor endplate in frog skeletal muscle in the 1950’s. Considering the morphological identification of synaptic vesicles (SVs) at the nerve terminals that appeared to be homogeneous in size, the hypothesis proposed that signal transduction at synapses is mediated by the release of neurotransmitters packed in SVs that are individually uniform in size; the amount of transmitter in a synaptic vesicle is called a quantum. Although quantal size—the amplitude of the postsynaptic response elicited by the release of neurotransmitters from a single vesicle—clearly depends on the number and sensitivity of the postsynaptic receptors, accumulating evidence has also indicated that the amount of neurotransmitters stored in SVs can be altered by various presynaptic factors. Here, I provide an overview of the concepts and underlying presynaptic molecular underpinnings that may regulate quantal size. PMID:26903855

  18. Quantal currents evoked by graded intracellular depolarization of crayfish motor axon terminals.

    PubMed Central

    Atwood, H L; Parnas, H; Parnas, I; Wojtowicz, J M

    1987-01-01

    1. Quantal transmitter release was examined at nerve terminals of the excitatory motor axon of the crayfish opener muscle. The magnitude of synaptic currents, recorded with macro-patch electrodes at a nerve terminal, served as a measure of quantal size. Transmitter release was initiated by pulses of depolarizing current applied intracellularly to the axonal terminals after application of tetrodotoxin. Quantal release was altered by a variety of methods and the resulting quantal output and quantal size were measured. 2. Amplitude distributions of quantal events were obtained during experimental manipulations which altered the rate of quantal release by up to 25-fold. These manipulations consisted of: varying pulse amplitude or pulse duration; facilitating the release by prolonged depolarization; and application of a potassium channel blocker, 4-aminopyridine. 3. The amplitude of quantal events is impervious to marked changes in presynaptic depolarization and is not affected by experimental procedures which promote accumulation of calcium ions in the terminals. The vesicular mechanism of release, in which transmitter substance is prepackaged in vesicles which individually undergo exocytosis at a release zone, could account for the observed results. PMID:2888878

  19. Synaptotagmin-7 Is Essential for Ca2+-Triggered Delayed Asynchronous Release But Not for Ca2+-Dependent Vesicle Priming in Retinal Ribbon Synapses

    PubMed Central

    Bacaj, Taulant

    2015-01-01

    Most synapses release neurotransmitters in two phases: (1) a fast synchronous phase lasting a few milliseconds; and (2) a delayed “asynchronous” phase lasting hundreds of milliseconds. Ca2+ triggers fast synchronous neurotransmitter release by binding to synaptotagmin-1, synaptotagmin-2, or synaptotagmin-9, but how Ca2+ triggers delayed asynchronous release has long remained enigmatic. Recent results suggested that consistent with the Ca2+-sensor function of synaptotagmin-7 in neuroendocrine exocytosis, synaptotagmin-7 also functions as a Ca2+ sensor for synaptic vesicle exocytosis but operates during delayed asynchronous release. Puzzlingly, a subsequent study postulated that synaptotagmin-7 is not a Ca2+ sensor for release but mediates Ca2+-dependent vesicle repriming after intense stimulation. To address these issues, we here analyzed synaptic transmission at rod bipolar neuron–AII amacrine cell synapses in acute mouse retina slices as a model system. Using paired recordings, we show that knock-out of synaptotagmin-7 selectively impairs delayed asynchronous release but not fast synchronous release. Delayed asynchronous release was blocked in wild-type synapses by intracellular addition of high concentrations of the slow Ca2+-chelator EGTA, but EGTA had no effect in synaptotagmin-7 knock-out neurons because delayed asynchronous release was already impaired. Moreover, direct measurements of vesicle repriming failed to uncover an effect of the synaptotagmin-7 knock-out on vesicle repriming. Our data demonstrate that synaptotagmin-7 is selectively essential for Ca2+-dependent delayed asynchronous release in retinal rod bipolar cell synapses, that its function can be blocked by simply introducing a slow Ca2+ buffer into the cells, and that synaptotagmin-7 is not required for normal vesicle repriming. SIGNIFICANCE STATEMENT How Ca2+ triggers delayed asynchronous release has long remained enigmatic. Synaptotagmin-7 has been implicated recently as Ca2+ sensor in

  20. TOTAL CULTURABLE VIRUS QUANTAL ASSAY

    EPA Science Inventory

    This chapter describes a quantal method for assaying culturable human enteric viruses from water matrices. The assay differs from the plaque assay described in Chapter 10 (December 1987 Revision) in that it is based upon the direct microscopic viewing of cells for virus-induced ...

  1. Quantal Cumulant Dynamics for Dissipative Systems

    SciTech Connect

    Shigeta, Yasuteru

    2007-12-26

    We develop a quantal cumulant dynamics method for the quantum tunneling in dissipative environment. Reduced equations of motion of classical and quantal cumulant variables without bath degrees of freedom are derived. We observed suppression of the tunneling that depends on the sign of a friction constant for an Ohmic approximation and on the magnitude of a bath frequency for a single bath mode approximation. A possible mechanism of the suppression is explored by analyzing an effective quantal potential of the tunneling path.

  2. The effect of opiates on the terminal nerve impulse and quantal secretion from visualized amphibian nerve terminals.

    PubMed Central

    Lavidis, N. A.

    1995-01-01

    1. Secretion of transmitter from amphibian motor nerve terminal release sites is intermittent, spatially non-uniform and varies considerably throughout the year and during development. The role of opioid receptors in modulating transmitter secretion from amphibian motor nerve terminals is evaluated in this study. 2. Dynorphin-A (24 microM) and morphine (500 microM) did not significantly change the shape of the nerve impulse or the consistency with which it was observed, but decreased evoked quantal secretion by more than 50%. These effects of dynorphin-A and morphine were largely reversed by naloxone (50 microM). 3. Dynorphin-A and morphine did not significantly change either the amplitude or the frequency of spontaneous quantal secretions. 4. There was a uniform decrease in evoked quantal secretion from release sites along terminal branches, irrespective of the quantal content value before drug treatment, indicating no difference in the susceptibility of proximal vs distal release sites to opiates. 5. Increasing the extracellular calcium concentration (0.3 to 0.4 mM) or trains of conditioning-test impulses (25 to 100 Hz) resulted in smaller dynorphin-A or morphine-induced decreases in evoked quantal secretion. 6. The decrease in evoked quantal secretion occurs as a result of a uniform decrease in the probability of quantal secretion from release sites without any affect on the propagation of the nerve terminal impulse. Low probability release sites become effectively silent. PMID:7582455

  3. Asynchronous GABA Release Is a Key Determinant of Tonic Inhibition and Controls Neuronal Excitability: A Study in the Synapsin II−/− Mouse

    PubMed Central

    Medrihan, Lucian; Ferrea, Enrico; Greco, Barbara; Baldelli, Pietro; Benfenati, Fabio

    2015-01-01

    Idiopathic epilepsies have frequently been linked to mutations in voltage-gated channels (channelopathies); recently, mutations in several genes encoding presynaptic proteins have been shown to cause epilepsy in humans and mice, indicating that epilepsy can also be considered a synaptopathy. However, the functional mechanisms by which presynaptic dysfunctions lead to hyperexcitability and seizures are not well understood. We show that deletion of synapsin II (Syn II), a presynaptic protein contributing to epilepsy predisposition in humans, leads to a loss of tonic inhibition in mouse hippocampal slices due to a dramatic decrease in presynaptic asynchronous GABA release. We also show that the asynchronous GABA release reduces postsynaptic cell firing, and the parallel impairment of asynchronous GABA release and tonic inhibition results in an increased excitability at both single-neuron and network levels. Restoring tonic inhibition with THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol; gaboxadol), a selective agonist of δ subunit-containing GABAA receptors, fully rescues the SynII−/− epileptic phenotype both ex vivo and in vivo. The results demonstrate a causal relationship between the dynamics of GABA release and the generation of tonic inhibition, and identify a novel mechanism of epileptogenesis generated by dysfunctions in the dynamics of release that can be effectively targeted by novel antiepileptic strategies. PMID:24962993

  4. Facilitation at single synapses probed with optical quantal analysis.

    PubMed

    Oertner, Thomas G; Sabatini, Bernardo L; Nimchinsky, Esther A; Svoboda, Karel

    2002-07-01

    Many synapses can change their strength rapidly in a use-dependent manner, but the mechanisms of such short-term plasticity remain unknown. To understand these mechanisms, measurements of neurotransmitter release at single synapses are required. We probed transmitter release by imaging transient increases in [Ca(2+)] mediated by synaptic N-methyl-D-aspartate receptors (NMDARs) in individual dendritic spines of CA1 pyramidal neurons in rat brain slices, enabling quantal analysis at single synapses. We found that changes in release probability, produced by paired-pulse facilitation (PPF) or by manipulation of presynaptic adenosine receptors, were associated with changes in glutamate concentration in the synaptic cleft, indicating that single synapses can release a variable amount of glutamate per action potential. The relationship between release probability and response size is consistent with a binomial model of vesicle release with several (>5) independent release sites per active zone, suggesting that multivesicular release contributes to facilitation at these synapses. PMID:12055631

  5. Quantal analysis of excitatory synapses in rat hippocampal CA1 in vitro during low-frequency depression.

    PubMed Central

    Larkman, A U; Jack, J J; Stratford, K J

    1997-01-01

    1. We have performed a detailed quantal analysis of excitatory postsynaptic potentials (EPSPs) evoked by minimal extracellular stimulation in the CA1 region of slices of adult rat hippocampus maintained in vitro. 2. EPSPs were evoked at 2-5 Hz, and the eight that were analysed all showed at least a 50% depression of mean peak amplitude during recording. 3. EPSP amplitude fluctuations were analysed by three methods: the use of amplitude frequency histograms with clear and reliable peaks where available, graphs of the EPSP (coefficient of variation)-2 against EPSP mean, and analysis of EPSP mean and standard deviation assuming simple binomial statistics with the number of release sites (N) kept constant but the quantal size (Q) and the release probability (Pr) allowed to vary over time. 4. The results of the three analysis procedures were in good agreement. Seven EPSPs showed a substantial reduction in the mean number of quanta released per trial, and in three cases this was the predominant mechanism of the depression. Five EPSPs showed a substantial decrease in Q. Values for N ranged between 3 and 18, with a median of 6; Pr ranged between 0.14 and 0.81 and Q between 66 and 275 microV. 5. We used the Q estimates from the binomial method to correct the recorded EPSP amplitudes for changes in quantal size over time. For seven out of the eight EPSPs, this rescaling procedure allowed histograms with clear peaks to be obtained from longer runs of data, or improved the sharpness of the peaks in histograms from all the recorded data. The improvement in peak sharpness was assessed using an autocorrelation-based method. The correction was much less successful if the Q estimates were obtained with a variant of the binomial method in which Pr was held constant and N was allowed to vary. 6. The only simple explanation for the success of the correction procedure is that changes in quantal size were a major factor in obscuring peaks in histograms based on large numbers of trials

  6. Secretory vesicle rebound hyperacidification and increased quantal size due to prolonged methamphetamine exposure

    PubMed Central

    Markov, Dmitriy; Mosharov, Eugene V.; Setlik, Wanda; Gershon, Michael D.; Sulzer, David

    2009-01-01

    Acute exposure to amphetamines collapses secretory vesicle pH gradients, which increases cytosolic catecholamine levels while decreases the quantal size of catecholamine release during fusion events. Amphetamine and methamphetamine, however, are retained in tissues over long durations. We used optical and electron microscopic probes to measure the effects of long-term methamphetamine exposure on secretory vesicle pH, and amperometry and intracellular patch electrochemistry to observe the effects on neurosecretion and cytosolic catecholamines in cultured rat chromaffin cells. In contrast to acute methamphetamine effects, exposure to the drug for 6–48 h at 10 μM and higher concentrations produced a concentration-dependent rebound hyperacidification of secretory vesicles. At 5–10 μM levels, methamphetamine increased the quantal size and reinstated exocytotic catecholamine release, although very high (>100 μM) levels of the drug, while continuing to produce rebound hyperacidification, did not increase quantal size. Secretory vesicle rebound hyperacidification was temperature dependent with optimal response at ~ 37°C, was not blocked by the transcription inhibitor, puromycin, and appears to be a general compensatory response to prolonged exposure with membranophilic weak bases, including amphetamines, methylphenidate, cocaine, and ammonia. Thus, under some conditions of prolonged exposure, amphetamines and other weak bases can enhance, rather than deplete, the vesicular release of catecholamines via a compensatory response resulting in vesicle acidification. PMID:19014382

  7. The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.

    PubMed

    Kobayashi, Shizuka; Hida, Yamato; Ishizaki, Hiroyoshi; Inoue, Eiji; Tanaka-Okamoto, Miki; Yamasaki, Miwako; Miyazaki, Taisuke; Fukaya, Masahiro; Kitajima, Isao; Takai, Yoshimi; Watanabe, Masahiko; Ohtsuka, Toshihisa; Manabe, Toshiya

    2016-09-01

    Synaptic efficacy is determined by various factors, including the quantal size, which is dependent on the amount of neurotransmitters in synaptic vesicles at the presynaptic terminal. It is essential for stable synaptic transmission that the quantal size is kept within a constant range and that synaptic efficacy during and after repetitive synaptic activation is maintained by replenishing release sites with synaptic vesicles. However, the mechanisms for these fundamental properties have still been undetermined. We found that the active zone protein CAST (cytomatrix at the active zone structural protein) played pivotal roles in both presynaptic regulation of quantal size and recycling of endocytosed synaptic vesicles. In the CA1 region of hippocampal slices of the CAST knockout mice, miniature excitatory synaptic responses were increased in size, and synaptic depression after prolonged synaptic activation was larger, which was attributable to selective impairment of synaptic vesicle trafficking via the endosome in the presynaptic terminal likely mediated by Rab6. Therefore, CAST serves as a key molecule that regulates dynamics and neurotransmitter contents of synaptic vesicles in the excitatory presynaptic terminal in the central nervous system. PMID:27422015

  8. Adaptation of quantal content to decreased postsynaptic sensitivity at single endplates in alpha-bungarotoxin-treated rats.

    PubMed Central

    Plomp, J J; van Kempen, G T; Molenaar, P C

    1992-01-01

    1. Rats were injected once every 48 h with alpha-bungarotoxin (alpha BTX) for periods up to 6 weeks. Injections caused weakness of facial muscles which lasted about 8 h. Hemidiaphragms were dissected for biochemical and electrophysiological measurements. 2. In muscles from animals treated for 2-3 weeks with toxin, the binding of 125I-alpha BTX was reduced to 58%, and the ACh content to 81% of control values. Choline acetyltransferase activity was unchanged. ACh release evoked by 3 Hz nerve stimulation was increased to 175% of control values. 3. The use of mu-conotoxin, which specifically blocks muscle action potentials, enabled the recording of full-sized endplate potentials (EPPs) and miniature endplate potentials (MEPPs) at normal muscle membrane potentials (-70 to -80 mV). The amplitude of MEPPs was decreased to 57% in muscles from animals treated for 3 weeks with alpha BTX. The mean of the quantal contents, calculated from the ratio of the corrected EPPs and the MEPPs, was increased to 154%. 4. Within individual muscles of both alpha BTX-treated and control rats, there was an inverse relationship between the quantal content of an endplate and its MEPP amplitude. 5. The MEPP frequency of endplates from control muscles was positively correlated with the quantal content. However, this correlation was not found in alpha BTX-affected muscles. 6. Three hours after a single injection of alpha BTX the amplitude of the MEPPs was reduced to about 60% of control values but no increase of the quantal content was found. During the first few days of alpha BTX treatment the quantal content gradually increased; it reached a plateau between 20 and 30 days. 7. The results suggest the existence of an adaptive mechanism, operating at individual endplates, in which retrograde signals at the motor nerve terminals modulate ACh release when neuromuscular transmission is endangered by block of acetylcholine receptors. PMID:1302275

  9. Distinct modes of dopamine and GABA release in a dual transmitter neuron

    PubMed Central

    Borisovska, Maria; Bensen, AeSoon; Chong, Gene; Westbrook, Gary L.

    2013-01-01

    We now know of a surprising number of cases where single neurons contain multiple neurotransmitters. Neurons that contain a fast-acting neurotransmitter such as glutamate or GABA, and a modulatory transmitter such as dopamine are a particularly interesting case because they presumably serve dual signaling functions. The olfactory bulb contains a large population of GABA and dopamine-containing neurons, which have been implicated in normal olfaction as well as in Parkinson’s disease. Yet, they have been classified as non-exocytotic catecholamine neurons because of the apparent lack of vesicular monoamine transporters. Thus we examined how dopamine is stored and released from tyrosine-hydroxylase-positive-GFP (TH+-GFP) mouse periglomerular neurons in vitro. TH+ cells expressed both VMAT2 and VGAT, consistent with vesicular storage of both dopamine and GABA. Carbon fiber amperometry revealed that release of dopamine was quantal and calcium-dependent, but quantal size was much less than expected for large dense core vesicles, suggesting that release originated from EM-identified small clear vesicles. A single action potential in a TH+ neuron evoked a brief GABA synaptic current whereas evoked dopamine release was asynchronous, lasting for tens of seconds. Our data suggests that dopamine and GABA serve temporally distinct roles in these dual transmitter neurons. PMID:23365218

  10. Parallel cone bipolar pathways to a ganglion cell use different rates and amplitudes of quantal excitation.

    PubMed

    Freed, M A

    2000-06-01

    The cone signal reaches the cat's On-beta (X) ganglion cell via several parallel circuits (bipolar cell types b1, b2, and b3). These circuits might convey different regions of the cone's temporal bandwidth. To test this, I presented a step of light that elicited a transient depolarization followed by a sustained depolarization. The contribution of bipolar cells to these response components was isolated by blocking action potentials with tetrodotoxin and by blocking inhibitory synaptic potentials with bicuculline and strychnine. Stationary fluctuation analysis of the sustained depolarization gave the rate of quantal bombardment: approximately 5100 quanta sec(-1) for small central cells and approximately 45,000 quanta sec(-1) for large peripheral cells. Normalizing these rates for the vastly different numbers of bipolar synapses (150-370 per small cell vs 2000 per large cell), quantal rate was constant across the retina, approximately 22 quanta synapse(-1) sec(-1). Nonstationary fluctuation analysis gave the mean quantal EPSP amplitude: approximately 240 microV for the transient depolarization and 30 microV for the sustained depolarization. The b1 bipolar cell is known from noise analysis of the On-alpha ganglion cell to have a near-maximal sustained release of only approximately two quanta synapse(-1) sec(-1). This implies that the other bipolar types (b2 and b3) contribute many more quanta to the sustained depolarization (>/=46 synapse(-1) sec(-1)). Type b1 probably contributes large quanta to the transient depolarization. Thus, bipolar cell types b1 and b2/b3 apparently constitute parallel circuits that convey, respectively, high and low frequencies. PMID:10818130

  11. The Evolution of Matter: The Quantal Unity of Evolution

    NASA Astrophysics Data System (ADS)

    Farre, George L.

    2002-09-01

    This paper is a brief sketch of the mechanisms that govern the construction of natural systems. Consequently, relatively little will be said about the external circumstances of their diversification. As nature has relatively few tricks in its bag, what follows may be viewed as an overview of the quantal unit of evolution (QUE), modally indexed.

  12. Computer Simulation of Quantal Dose-Response Relationships.

    ERIC Educational Resources Information Center

    McGilliard, Kip L.

    1985-01-01

    Describes a program which simulates animal pharmacology experiments involving "all-or-none" responses. Use of the Applesoft BASIC program in the pharmacology teaching laboratory provides students with a rapid and economical way to gain experience in the design and statistical analysis of quantal dose-response experiments. Information on obtaining…

  13. Analysis of quantal size of voltage responses to retinal stimulation in the accessory optic system.

    PubMed

    Ariel, Michael; Johny, Manu Ben

    2007-07-01

    In the intact vertebrate central nervous system, the quantal nature of synaptic transmission is difficult to measure because the postsynaptic sites may be distributed along a tortuous dendritic tree that cannot be readily clamped spatially to a uniform potential. Titrating the intact brain's extracellular concentration of calcium ions is also challenging because of its strong buffering mechanisms. In this study, using a whole brain with eye attached preparation, quantal neurotransmission was examined in the turtle brainstem in vitro, by recording from accessory optic system neurons that receive direct input from visually responsive retinal ganglion cells. Unitary EPSPs, evoked by microstimulation of a single ganglion cell, were measured during whole cell current-clamp recordings. In this preparation, the neurons exhibit direction-selectivity, despite the hypoxic conditions. Bath application of cadmium to reduce calcium influx also reduced evoked EPSP amplitudes to that of the spontaneous synaptic events. Statistical analyses indicated that these evoked response amplitudes could be well fitted to a Poisson distribution for most brainstem neurons. Therefore, the spontaneous miniature excitatory synaptic events of approximately 1 mV, as also observed during spike blockade of the retina [Kogo, N., Ariel, M., 1997. Membrane properties and monosynaptic retinal excitation of neurons in the turtle accessory optic system. Journal of Neurophysiology 78, 614-627], are likely responses to the neurotransmitter of single vesicles release by retinal axon terminals. PMID:17543898

  14. Quantal Heating of Conducting Electrons with Discrete Spectrum

    SciTech Connect

    Vitkalov, S. A.; Bykov, A. A.

    2011-12-23

    Usually heating of conducting electrons by dc electric field results in an increase of electron temperature. In this paper we show that the dc heating of 2D electrons, placed in quantized magnetic fields, results in a peculiar electron distribution, which has the same broadening or an effective 'temperature' as the unbiased electron system. The quantal heating, however, violates strongly the Ohm's Law. In the conducting system with discrete electron spectrum the quantal heating results in spectacular decrease of electron resistance and transition of the electrons into a state with zero differential resistance (ZDR). Finally the heating leads to apparent dc driven metal-insulator transition, which correlates with the transition into the ZDR state. The correlation is very unexpected and is not understood.

  15. Asynchronous FPGA risks

    NASA Technical Reports Server (NTRS)

    Erickson, K.

    2000-01-01

    The worst case timing margin of a synchronous design implemented with a field-programmable gate array (FPGA) is easy to perform using available FPGA design tools. However, it may be difficult to impossible to verify that worst case timing requirements are met for complex asynchronous logic design.

  16. A Computer Simulation for Teaching Quantal Time Development

    NASA Astrophysics Data System (ADS)

    Styer, Daniel F.

    1996-11-01

    The computer program QMTime (for MS-DOS machines) enables students to simulate quantal time development in one dimension. A variety of initial wave packets (Gaussian, Lorentzian, etc.) can evolve in time under the influence of a variety of potential energy functions (step, ramp, square well, harmonic oscillator, etc.) with or without an external driving force. A novel visualization technique simultaneously displays the magnitude and phase of complex-valued wavefunctions. Either position-space or momentum-space wavefunctions, or both, can be shown. The program is particularly effective in demonstrating the classical limit of quantum mechanics. This program is part of the CUPS (Consortium for Upper level Physics Software) project.

  17. Dose-response model for teratological experiments involving quantal responses

    SciTech Connect

    Rai, K.; Van Ryzin, J.

    1985-03-01

    This paper introduces a dose-response model for teratological quantal response data where the probability of response for an offspring from a female at a given dose varies with the litter size. The maximum likelihood estimators for the parameters of the model are given as the solution of a nonlinear iterative algorithm. Two methods of low-dose extrapolation are presented, one based on the litter size distribution and the other a conservative method. The resulting procedures are then applied to a teratological data set from the literature.

  18. Dynamics of quantal heating in electron systems with discrete spectra

    NASA Astrophysics Data System (ADS)

    Dietrich, Scott; Mayer, William; Vitkalov, Sergey; Bykov, A. A.

    2015-05-01

    The temporal evolution of quantal Joule heating of two-dimensional (2D) electrons in a GaAs quantum well placed in quantizing magnetic fields is studied using a difference-frequency method. The method is based on measurements of the electron conductivity oscillating at the beat frequency f =f1-f2 between two microwaves applied to the 2D system at frequencies f1 and f2. The method provides direct access to the dynamical characteristics of the heating and yields the inelastic-scattering time τi n of 2D electrons. The obtained τi n is strongly temperature dependent, varying from 0.13 ns at 5.5 K to 1 ns at 2.4 K in magnetic field B =0.333 T . When the temperature T exceeds the Landau-level separation, the relaxation rate 1 /τi n is proportional to T2, indicating electron-electron interaction as the dominant mechanism limiting the quantal heating. At lower temperatures, the rate tends to be proportional to T3, indicating considerable contribution from electron-phonon scattering.

  19. Preferential cell attachment to nitrogen-doped diamond-like carbon (DLC:N) for the measurement of quantal exocytosis

    PubMed Central

    Sen, Atanu; Barizuddin, Syed; Hossain, Maruf; Polo-Parada, Luis; Gillis, Kevin D.; Gangopadhyay, Shubhra

    2013-01-01

    Electrochemical measurement of transmitter or hormone release from individual cells on microchips has applications both in basic science and drug screening. High-resolution measurement of quantal exocytosis requires the working electrode to be small (cell-sized) and located in immediate proximity to the cell. We examined the ability of candidate electrode materials to promote the attachment of two hormone-secreting cell types as a mechanism for targeting cells for to recording electrodes with high precision. We found that nitrogen-doped diamond-like carbon (DLC:N) promoted cell attachment relative to other materials tested in the rank order of DLC:N > In2O3/SnO2 (ITO), Pt > Au. In addition, we found that treating candidate electrode materials with polylysine did not increase attachment of chromaffin cells to DLC:N, but promoted cell attachment to the other tested materials. We found that hormone-secreting cells did not attach readily to Teflon AF as a potential insulating material, and demonstrated that patterning of Teflon AF leads to selective cell targeting to DLC:N “docking sites”. These results will guide the design of the next generation of biochips for automated and high-throughput measurement of quantal exocytosis. PMID:19124153

  20. Nanocrystalline diamond microelectrode arrays fabricated on sapphire technology for high-time resolution of quantal catecholamine secretion from chromaffin cells.

    PubMed

    Carabelli, V; Gosso, S; Marcantoni, A; Xu, Y; Colombo, E; Gao, Z; Vittone, E; Kohn, E; Pasquarelli, A; Carbone, E

    2010-09-15

    The quantal release of oxidizable molecules can be successfully monitored by means of polarized carbon fiber microelectrodes (CFEs) positioned in close proximity to the cell membrane. To partially overcome certain CFE limitations, mainly related to their low spatial resolution and lack of optical transparency, we developed a planar boron-doped nanocrystalline diamond (NCD) prototype, grown on a transparent sapphire wafer. Responsiveness to applied catecholamines as well as the electrochemical and optical properties of the NCD-based device were first characterized by cyclic voltammetry and optical transmittance measurements. By stimulating chromaffin cells positioned on the device with external KCl, well-resolved quantal exocytotic events could be detected either from one NCD microelectrode, or simultaneously from an array of four microelectrodes, indicating that the chip is able to monitor secretory events (amperometric spikes) from a number of isolated chromaffin cells. Spikes detected by the planar NCD device had comparable amplitudes, kinetics and vesicle diameter distributions as those measured by conventional CFEs from the same chromaffin cell. PMID:20570501

  1. Measurement of quantal secretion induced by ouabain and its correlation with depletion of synaptic vesicles.

    PubMed

    Haimann, C; Torri-Tarelli, F; Fesce, R; Ceccarelli, B

    1985-11-01

    Ouabain (0.1 and 0.05 mM) was applied to frog cutaneous pectoris nerve-muscle preparations bathed in modified Ringer's solution containing either 1.8 mM Ca2+ (and 4 mM Mg2+) or no added Ca2+ (4 mM Mg2+ and 1 mM EGTA). During the intense quantal release of acetylcholine (ACh) induced by ouabain, the parameters of the miniature endplate potentials (mepps) were deduced from the variance, skew, and power spectra of the endplate recordings by applying a recently described modification of classical fluctuation analysis. Often the high frequency of mepps is not stationary; therefore, the signal was high-pass filtered (time constant of the resistance-capacitance filter of 2 ms) to remove the errors introduced by nonstationarity. When ouabain was applied in the presence of Ca2+, mepp frequency started to rise exponentially after a lag of 1.5-2 h, reached an average peak frequency of 1,300/s in approximately 30 min, and then suddenly subsided to low level (10/s). In Ca2+-free solution, after a shorter lag (1-1.5 h), mepp frequency rose to peak rate of 700/s in approximately 20 min and then gradually subsided. In spite of the different time course of secretion in the two experimental conditions, the cumulative quantal release was not significantly different (7.4 +/- 1.3 X 10(5) in Ca2+-containing and 8.8 +/- 2.7 X 10(5) in Ca2+-free solutions). 60 min after the peak secretion, the muscles were fixed for observation in the electron microscope. Morphometric analysis on micrographs of neuromuscular junctions revealed in both cases a profound depletion of synaptic vesicles and deep infoldings of presynaptic membrane. This rapid depletion and the lack of uptake of horseradish peroxidase suggest that ouabain impairs the recycling process that tends to conserve the vesicle population during intense secretion of neurotransmitter. The good correlation observed between the reduction in the store of synaptic vesicles and the total number of quanta of ACh secreted in the absence of a

  2. A Microscopic Quantal Model for Nuclear Collective Rotation

    SciTech Connect

    Gulshani, P.

    2007-10-26

    A microscopic, quantal model to describe nuclear collective rotation in two dimensions is derived from the many-nucleon Schrodinger equation. The Schrodinger equation is transformed to a body-fixed frame to decompose the Hamiltonian into a sum of intrinsic and rotational components plus a Coriolis-centrifugal coupling term. This Hamiltonian (H) is expressed in terms of space-fixed-frame particle coordinates and momenta by using commutator of H with a rotation angle. A unified-rotational-model type wavefunction is used to obtain an intrinsic Schrodinger equation in terms of angular momentum quantum number and two-body operators. A Hartree-Fock mean-field representation of this equation is then obtained and, by means of a unitary transformation, is reduced to a form resembling that of the conventional semi-classical cranking model when exchange terms and intrinsic spurious collective excitation are ignored.

  3. Labeled Postings for Asynchronous Interaction

    ERIC Educational Resources Information Center

    ChanLin, Lih-Juan; Chen, Yong-Ting; Chan, Kung-Chi

    2009-01-01

    The Internet promotes computer-mediated communications, and so asynchronous learning network systems permit more flexibility in time, space, and interaction than synchronous mode of learning. The key point of asynchronous learning is the materials for web-aided teaching and the flow of knowledge. This research focuses on improving online…

  4. Asynchronous & Synchronous E-Learning

    ERIC Educational Resources Information Center

    Hrastinski, Stefan

    2008-01-01

    An ongoing debate addresses the usefulness of asynchronous versus synchronous e-learning. "Asynchronous e-learning," commonly facilitated by media such as e-mail and discussion boards, supports work relations among learners and with teachers, even when participants cannot be online at the same time. "Synchronous e-learning," commonly supported by…

  5. The quantal component of synaptic transmission from sensory hair cells to the vestibular calyx.

    PubMed

    Highstein, Stephen M; Mann, Mary Anne; Holstein, Gay R; Rabbitt, Richard D

    2015-06-01

    Spontaneous and stimulus-evoked excitatory postsynaptic currents (EPSCs) were recorded in calyx nerve terminals from the turtle vestibular lagena to quantify key attributes of quantal transmission at this synapse. On average, EPSC events had a magnitude of ∼ 42 pA, a rise time constant of τ(0) ∼ 229 μs, decayed to baseline with a time constant of τ(R) ∼ 690 μs, and carried ∼ 46 fC of charge. Individual EPSCs varied in magnitude and decay time constant. Variability in the EPSC decay time constant was hair cell dependent and due in part to a slow protraction of the EPSC in some cases. Variability in EPSC size was well described by an integer summation of unitary quanta, with each quanta of glutamate gating a unitary postsynaptic current of ∼ 23 pA. The unitary charge was ∼ 26 fC for EPSCs with a simple exponential decay and increased to ∼ 48 fC for EPSCs exhibiting a slow protraction. The EPSC magnitude and the number of simultaneous unitary quanta within each event increased with presynaptic stimulus intensity. During tonic hair cell depolarization, both the EPSC magnitude and event rate exhibited adaptive run down over time. Present data from a reptilian calyx are remarkably similar to noncalyceal vestibular synaptic terminals in diverse species, indicating that the skewed EPSC size distribution and multiquantal release might be an ancestral property of inner ear ribbon synapses. PMID:25878150

  6. The quantal component of synaptic transmission from sensory hair cells to the vestibular calyx

    PubMed Central

    Highstein, Stephen M.; Mann, Mary Anne; Holstein, Gay R.

    2015-01-01

    Spontaneous and stimulus-evoked excitatory postsynaptic currents (EPSCs) were recorded in calyx nerve terminals from the turtle vestibular lagena to quantify key attributes of quantal transmission at this synapse. On average, EPSC events had a magnitude of ∼42 pA, a rise time constant of τ0 ∼229 μs, decayed to baseline with a time constant of τR ∼690 μs, and carried ∼46 fC of charge. Individual EPSCs varied in magnitude and decay time constant. Variability in the EPSC decay time constant was hair cell dependent and due in part to a slow protraction of the EPSC in some cases. Variability in EPSC size was well described by an integer summation of unitary quanta, with each quanta of glutamate gating a unitary postsynaptic current of ∼23 pA. The unitary charge was ∼26 fC for EPSCs with a simple exponential decay and increased to ∼48 fC for EPSCs exhibiting a slow protraction. The EPSC magnitude and the number of simultaneous unitary quanta within each event increased with presynaptic stimulus intensity. During tonic hair cell depolarization, both the EPSC magnitude and event rate exhibited adaptive run down over time. Present data from a reptilian calyx are remarkably similar to noncalyceal vestibular synaptic terminals in diverse species, indicating that the skewed EPSC size distribution and multiquantal release might be an ancestral property of inner ear ribbon synapses. PMID:25878150

  7. Asynchronous parallel status comparator

    DOEpatents

    Arnold, Jeffrey W.; Hart, Mark M.

    1992-01-01

    Apparatus for matching asynchronously received signals and determining whether two or more out of a total number of possible signals match. The apparatus comprises, in one embodiment, an array of sensors positioned in discrete locations and in communication with one or more processors. The processors will receive signals if the sensors detect a change in the variable sensed from a nominal to a special condition and will transmit location information in the form of a digital data set to two or more receivers. The receivers collect, read, latch and acknowledge the data sets and forward them to decoders that produce an output signal for each data set received. The receivers also periodically reset the system following each scan of the sensor array. A comparator then determines if any two or more, as specified by the user, of the output signals corresponds to the same location. A sufficient number of matches produces a system output signal that activates a system to restore the array to its nominal condition.

  8. Asynchronous interactive control systems

    NASA Technical Reports Server (NTRS)

    Vuskovic, M. I.; Heer, E.

    1980-01-01

    A class of interactive control systems is derived by generalizing interactive manipulator control systems. The general structural properties of such systems are discussed and an appropriate general software implementation is proposed. This is based on the fact that tasks of interactive control systems can be represented as a network of a finite set of actions which have specific operational characteristics and specific resource requirements, and which are of limited duration. This has enabled the decomposition of the overall control algorithm into a set of subalgorithms, called subcontrollers, which can operate simultaneously and asynchronously. Coordinate transformations of sensor feedback data and actuator set-points have enabled the further simplification of the subcontrollers and have reduced their conflicting resource requirements. The modules of the decomposed control system are implemented as parallel processes with disjoint memory space communicating only by I/O. The synchronization mechanisms for dynamic resource allocation among subcontrollers and other synchronization mechanisms are also discussed in this paper. Such a software organization is suitable for the general form of multiprocessing using computer networks with distributed storage.

  9. Creating Asynchronous Online Learning Communities

    ERIC Educational Resources Information Center

    Kerr, Crystal

    2009-01-01

    This research project examined how to develop and sustain online, asynchronous learning communities in continuous intake, distance education environments for learners in grades 7 through 10. The study is an action research project that is based upon in-depth, qualitative data. Interviews were conducted with distance education teachers,…

  10. Student Satisfaction with Asynchronous Learning

    ERIC Educational Resources Information Center

    Dziuban, Charles; Moskal, Patsy; Brophy, Jay; Shea, Peter

    2007-01-01

    The authors discuss elements that potentially impact student satisfaction with asynchronous learning: the media culture, digital, personal and mobile technologies, student learning preferences, pedagogy, complexities of measurement, and the digital generation. They describe a pilot study to identify the underlying dimensions of student…

  11. Acquiring Knowledge from Asynchronous Discussion

    ERIC Educational Resources Information Center

    Teo, Yiong Hwee; Webster, Len

    2008-01-01

    This article discusses a study which was designed to explore how online scaffolding can be incorporated to support knowledge acquisition in asynchronous discussion. A group of Singapore preservice teachers engaged in collaborative critiquing of videos before they embarked on their video projects to illustrate what constitutes good and bad video…

  12. Variability of Vowel Formant Frequencies and the Quantal Theory of Speech: A First Report

    PubMed Central

    Pisoni, David B.

    2012-01-01

    This paper reports the results of a study in which variability of formant frequencies for different vowels was examined with regard to several predictions derived from the quantal theory of speech. Two subjects were required to reproduce eight different steady-state synthetic vowels which were presented repeatedly in a randomized order. Spectral analysis was carried out on the vocal responses in order to obtain means and standard deviations of the vowel formant frequencies. In the spirit of the quantal theory, it was predicted that the points vowel, /i/, /a/ and /u/ would show lower standard deviations than the nonpoint vowels because these vowels are assumed to be produced at places in the vocal tract where small perturbations in articulation produce only minimal changes in the resulting formant frequencies. That is, these vowels are assumed to be quantal vowels. The results of this study provided little support for the hypothesis under consideration. A discussion of the outcome of the results as well as some speculation as to its failure to find support for the quantal theory is provided in the report. Several final comments are also offered about computer simulation studies of speech production and the need for additional empirical studies on vowel production with real talkers. PMID:7280032

  13. Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources.

    PubMed

    Wen, Hua; Hubbard, Jeffrey M; Rakela, Benjamin; Linhoff, Michael W; Mandel, Gail; Brehm, Paul

    2013-01-01

    Asynchronous transmission plays a prominent role at certain synapses but lacks the mechanistic insights of its synchronous counterpart. The current view posits that triggering of asynchronous release during repetitive stimulation involves expansion of the same calcium domains underlying synchronous transmission. In this study, live imaging and paired patch clamp recording at the zebrafish neuromuscular synapse reveal contributions by spatially distinct calcium sources. Synchronous release is tied to calcium entry into synaptic boutons via P/Q type calcium channels, whereas asynchronous release is boosted by a propagating intracellular calcium source initiated at off-synaptic locations in the axon and axonal branch points. This secondary calcium source fully accounts for the persistence following termination of the stimulus and sensitivity to slow calcium buffers reported for asynchronous release. The neuromuscular junction and CNS neurons share these features, raising the possibility that secondary calcium sources are common among synapses with prominent asynchronous release. DOI: http://dx.doi.org/10.7554/eLife.01206.001. PMID:24368731

  14. Process membership in asynchronous environments

    NASA Technical Reports Server (NTRS)

    Ricciardi, Aleta M.; Birman, Kenneth P.

    1993-01-01

    The development of reliable distributed software is simplified by the ability to assume a fail-stop failure model. The emulation of such a model in an asynchronous distributed environment is discussed. The solution proposed, called Strong-GMP, can be supported through a highly efficient protocol, and was implemented as part of a distributed systems software project at Cornell University. The precise definition of the problem, the protocol, correctness proofs, and an analysis of costs are addressed.

  15. Developing asynchronous online interprofessional education.

    PubMed

    Sanborn, Heidi

    2016-09-01

    For many health programmes, developing interprofessional education (IPE) has been a challenge. Evidence on the best method for design and implementation of IPE has been slow to emerge, with little research on how to best incorporate IPE in the asynchronous online learning environment. This leaves online programmes with no clear guidance when embarking upon an initiative to integrate IPE into the curriculum. One tool that can be effective at guiding the incorporation of IPE across all learning platforms is the Interprofessional Education Collaborative (IPEC) competencies. A project was designed to integrate the nationally defined IPEC competencies throughout an asynchronous, online baccalaureate nursing completion programme. A programme-wide review led to targeted revision of course and unit-level objectives, learning experiences, and assessments based on the IPEC framework. As a result of this effort, the programme curriculum now provides interprofessional learning activities across all courses. This report provides a method for using the IPEC competencies to incorporate IPE within various asynchronous learning assessments, assuring students learn about, with, and from other professions. PMID:27310063

  16. Partition algebraic design of asynchronous sequential circuits

    NASA Technical Reports Server (NTRS)

    Maki, Gary K.; Chen, Kristen Q.; Gopalakrishnan, Suresh K.

    1993-01-01

    Tracey's Theorem has long been recognized as essential in generating state assignments for asynchronous sequential circuits. This paper shows that partitioning variables derived from Tracey's Theorem also has a significant impact in generating the design equations. Moreover, this theorem is important to the fundamental understanding of asynchronous sequential operation. The results of this work simplify asynchronous logic design. Moreover, detection of safe circuits is made easier.

  17. Thermal and quantal isospin and spin fluctuations in heavy ion reactions

    SciTech Connect

    Moretto, L.G.

    1980-01-01

    The isobaric charge distributions are discussed in terms of quantal and classical isospin fluctuations. The roles of mass asymmetry and of the higher giant isovector modes are treated within the framework of a cylinder model that is worked out exactly. Spin fluctuations are considered first in terms of quantal fluctuations in a cylinder model and second in terms of thermal fluctuations in a two-sphere model. The results are applied to the calculation of in- and out-of-plane angular distributions for sequential fission, alpha and gamma decay. Analytical expressions are obtained for the angular distributions. The theoretical predictions are compared with experimental results for sequential fission, alpha, and gamma angular distributions. 23 figures.

  18. Automated Targeting of Cells to Electrochemical Electrodes Using a Surface Chemistry Approach for the Measurement of Quantal Exocytosis

    PubMed Central

    2010-01-01

    Here, we describe a method to fabricate a multichannel high-throughput microchip device for the measurement of quantal transmitter release from individual cells. Instead of bringing carbon-fiber electrodes to cells, the device uses a surface chemistry approach to bring cells to an array of electrochemical microelectrodes. The microelectrodes are small and cytophilic in order to promote adhesion of a single cell, whereas all other areas of the chip are covered with a thin cytophobic film to block cell attachement and facilitate the movement of cells to electrodes. This cytophobic film also insulates unused areas of the conductive film; thus, the alignment of cell docking sites to working electrodes is automatic. Amperometric spikes resulting from single-granule fusion events were recorded on the device and had amplitudes and kinetics similar to those measured using carbon-fiber microelectrodes. Use of this device will increase the pace of basic neuroscience research and may also find applications in drug discovery or validation. PMID:21113333

  19. A new diamond biosensor with integrated graphitic microchannels for detecting quantal exocytic events from chromaffin cells.

    PubMed

    Picollo, Federico; Gosso, Sara; Vittone, Ettore; Pasquarelli, Alberto; Carbone, Emilio; Olivero, Paolo; Carabelli, Valentina

    2013-09-14

    An MeV ion-microbeam lithographic technique can be successfully employed for the fabrication of an all-carbon miniaturized cellular biosensor based on graphitic microchannels embedded in a single-crystal diamond matrix. The device is functionally characterized for the in vitro recording of quantal exocytic events from single chromaffin cells, with high sensitivity and signal-to-noise ratio, opening promising perspectives for the realization of monolithic all-carbon cellular biosensors. PMID:23847004

  20. Calcitonin gene-related peptide increases acetylcholine quantal size in neuromuscular junctions of mice.

    PubMed

    Gaydukov, Alexander E; Bogacheva, Polina O; Balezina, Olga P

    2016-08-15

    We used an intracellular microelectrode technique to study the mechanisms of action of two isoforms (human and rat) of calcitonin gene-related peptide (CGRP) on the evoked and spontaneous quantal secretion of acetylcholine (ACh) in mouse diaphragm motor synapses. Recordings of miniature endplate potentials (MEPPs) and evoked multiquantal endplate potentials (EPPs) in a cut neuromuscular preparation showed that CGRP increased the amplitude of EPPs without influencing their quantal content. Both isoforms of CGRP in a wide range of concentrations (1nM-1μM) provoked a similar considerable increase in MEPPs amplitude in a dose-dependent manner (up to 150-160% compared to control) without changing their frequency, rise-time, and decay. Inhibition of CGRP-receptors by truncated CGRP (CGRP8-37) completely prevented the potentiating effect of CGRP on the MEPPs amplitude. The effect of CGRP was not accompanied by changes in input resistance of muscle fiber membrane but was fully prevented by inhibition of vesicular ACh transport by vesamicol. Inhibition of protein kinase A (PKA) by H-89 also prevented CGRP action on the MEPPs amplitude. It is concluded that, in mammalian neuromuscular junctions, different isoforms of exogenously applied CGRP uniformly potentiate amplitudes of evoked and spontaneous postsynaptic potentials acting presynaptically via an increase in ACh quantal size. PMID:27288020

  1. Independent Sources of Quantal Variability at Single Glutamatergic Synapses

    PubMed Central

    Franks, Kevin M.; Stevens, Charles F.; Sejnowski, Terrence J.

    2010-01-01

    Variability in the size of single postsynaptic responses is a feature of most central neurons, although the source of this variability is not completely understood. The dominant source of variability could be either intersynaptic or intrasynaptic. To quantitatively examine this question, a biophysically realistic model of an idealized central axospinous synapse was used to assess mechanisms underlying synaptic variability measurements. Three independent sources of variability were considered: stochasticity of postsynaptic receptors (“channel noise”), variations of glutamate concentration in the synaptic cleft (Δq), and differences in the potency of vesicles released from different locations on the active zone [release-location dependence (RLD)]. As expected, channel noise was small (8% of the total variance) and Δq was the dominant source of variability (58% of total variance). Surprisingly, RLD accounted for a significant amount of variability (36%). Our simulations show that potency of release sites decreased with a length constant of ~100 nm, and that receptors were not activated by release events >300 nm away, which is consistent with the observation that single active zones are rarely >300 nm. RLD also predicts that the manner in which receptors are added or removed from synapses can dramatically affect the nature of the synaptic response, with increasing receptor density being more efficient than merely increasing synaptic area. Saturation levels and synaptic geometry were also important in determining the size and shape of the distribution of amplitudes recorded at different synapses. PMID:12716926

  2. Improved self arbitrated VLSI asynchronous circuits

    NASA Technical Reports Server (NTRS)

    Winterrowd, P.

    1991-01-01

    This paper introduces an improved method for designing the class of CMOS VLSI asynchronous sequential circuits introduced in the paper by Sterling R. Whitaker and Gary K. Maki, 'Self Arbitrated VLSI Asynchronous Circuits.' Of main interest here is the simple design by inspection rules that arise from these circuits. This paper presents a variation on these circuits which reduces the number of transistors required.

  3. Asynchronous Learning Forums for Business Acculturation

    ERIC Educational Resources Information Center

    Pence, Christine Cope; Wulf, Catharina

    2009-01-01

    The use of IT as a facilitator for student collaboration in higher business education has grown rapidly since 2000. Asynchronous discussion forums are used abundantly for collaborative training purposes and for teaching students business-relevant tools for their future careers. This article presents an analysis of the asynchronous discussion forum…

  4. Asynchronous Discussions and Assessment in Online Learning

    ERIC Educational Resources Information Center

    Vonderwell, Selma; Liang, Xin; Alderman, Kay

    2007-01-01

    This case study explored asynchronous online discussions, assessment processes, and the meaning students derived from their experiences in five online graduate courses at the Colleges of Education of two Midwestern higher education institutions. The findings suggest that asynchronous online discussions facilitate a multidimensional process of…

  5. An Asynchronous Augmentation to Traditional Course Delivery.

    ERIC Educational Resources Information Center

    Wolverton, Marvin L.; Wolverton, Mimi

    Asynchronous augmentation facilitates distributed learning, which relies heavily on technology and self-learning. This paper reports the results of delivering a real estate principles course using an asynchronous course delivery format. It highlights one of many ways to enhance learning using technology, and it provides information concerning how…

  6. Synaptotagmin-7 Is an Asynchronous Calcium Sensor for Synaptic Transmission in Neurons Expressing SNAP-23

    PubMed Central

    Weber, Jens P.; Toft-Bertelsen, Trine L.; Mohrmann, Ralf; Delgado-Martinez, Ignacio; Sørensen, Jakob B.

    2014-01-01

    Synchronization of neurotransmitter release with the presynaptic action potential is essential for maintaining fidelity of information transfer in the central nervous system. However, synchronous release is frequently accompanied by an asynchronous release component that builds up during repetitive stimulation, and can even play a dominant role in some synapses. Here, we show that substitution of SNAP-23 for SNAP-25 in mouse autaptic glutamatergic hippocampal neurons results in asynchronous release and a higher frequency of spontaneous release events (mEPSCs). Use of neurons from double-knock-out (SNAP-25, synaptotagmin-7) mice in combination with viral transduction showed that SNAP-23-driven release is triggered by endogenous synaptotagmin-7. In the absence of synaptotagmin-7 release became even more asynchronous, and the spontaneous release rate increased even more, indicating that synaptotagmin-7 acts to synchronize release and suppress spontaneous release. However, compared to synaptotagmin-1, synaptotagmin-7 is a both leaky and asynchronous calcium sensor. In the presence of SNAP-25, consequences of the elimination of synaptotagmin-7 were small or absent, indicating that the protein pairs SNAP-25/synaptotagmin-1 and SNAP-23/synaptotagmin-7 might act as mutually exclusive calcium sensors. Expression of fusion proteins between pHluorin (pH-sensitive GFP) and synaptotagmin-1 or -7 showed that vesicles that fuse using the SNAP-23/synaptotagmin-7 combination contained synaptotagmin-1, while synaptotagmin-7 barely displayed activity-dependent trafficking between vesicle and plasma membrane, implying that it acts as a plasma membrane calcium sensor. Overall, these findings support the idea of alternative syt∶SNARE combinations driving release with different kinetics and fidelity. PMID:25422940

  7. Carrying Synchronous Voice Data On Asynchronous Networks

    NASA Technical Reports Server (NTRS)

    Bergman, Larry A.

    1990-01-01

    Buffers restore synchronism for internal use and permit asynchronism in external transmission. Proposed asynchronous local-area digital communication network (LAN) carries synchronous voice, data, or video signals, or non-real-time asynchronous data signals. Network uses double buffering scheme that reestablishes phase and frequency references at each node in network. Concept demonstrated in token-ring network operating at 80 Mb/s, pending development of equipment operating at planned data rate of 200 Mb/s. Technique generic and used with any LAN as long as protocol offers deterministic (or bonded) access delays and sufficient capacity.

  8. Automatic defensive control of asynchronous sequential machines

    NASA Astrophysics Data System (ADS)

    Hammer, Jacob

    2016-01-01

    Control theoretic techniques are utilised to develop automatic controllers that counteract robotic adversarial interventions in the operation of asynchronous sequential machines. The scenario centres on automatic protection against pre-programmed adversarial agents that attempt to subvert the operation of an asynchronous computing system. Necessary and sufficient conditions for the existence of defensive controllers that automatically defeat such adversarial agents are derived. These conditions are stated in terms of skeleton matrices - matrices of zeros and ones obtained directly from the given description of the asynchronous sequential machine being protected. When defensive controllers exist, a procedure for their design is outlined.

  9. A Bayesian approach to the analysis of quantal bioassay studies using nonparametric mixture models.

    PubMed

    Fronczyk, Kassandra; Kottas, Athanasios

    2014-03-01

    We develop a Bayesian nonparametric mixture modeling framework for quantal bioassay settings. The approach is built upon modeling dose-dependent response distributions. We adopt a structured nonparametric prior mixture model, which induces a monotonicity restriction for the dose-response curve. Particular emphasis is placed on the key risk assessment goal of calibration for the dose level that corresponds to a specified response. The proposed methodology yields flexible inference for the dose-response relationship as well as for other inferential objectives, as illustrated with two data sets from the literature. PMID:24354490

  10. Cholinergic activation of the murine trachealis muscle via non-vesicular acetylcholine release involving low-affinity choline transporters.

    PubMed

    Nassenstein, Christina; Wiegand, Silke; Lips, Katrin S; Li, Guanfeng; Klein, Jochen; Kummer, Wolfgang

    2015-11-01

    In addition to quantal, vesicular release of acetylcholine (ACh), there is also non-quantal release at the motor endplate which is insufficient to evoke postsynaptic responses unless acetylcholinesterase (AChE) is inhibited. We here addressed potential non-quantal release in the mouse trachea by organ bath experiments and (immuno)histochemical methods. Electrical field stimulation (EFS) of nerve terminals elicited tracheal constriction that is largely due to ACh release. Classical enzyme histochemistry demonstrated acetylcholinesterase (AChE) activity in nerve fibers in the muscle and butyrylcholinesterase (BChE) activity in the smooth muscle cells. Acute inhibition of both esterases by eserine significantly raised tracheal tone which was fully sensitive to atropine. This effect was reduced, but not abolished, in AChE, but not in BChE gene-deficient mice. The eserine-induced increase in tracheal tone was unaffected by vesamicol (10(-5)M), an inhibitor of the vesicular acetylcholine transporter, and by corticosterone (10(-4)M), an inhibitor of organic cation transporters. Hemicholinium-3, in low concentrations an inhibitor of the high-affinity choline transporter-1 (CHT1), completely abrogated the eserine effects when applied in high concentrations (10(-4)M) pointing towards an involvement of low-affinity choline transporters. To evaluate the cellular sources of non-quantal ACh release in the trachea, expression of low-affinity choline transporter-like family (CTL1-5) was evaluated by RT-PCR analysis. Even though these transporters were largely abundant in the epithelium, denudation of airway epithelial cells had no effect on eserine-induced tracheal contraction, indicating a non-quantal release of ACh from non-epithelial sources in the airways. These data provide evidence for an epithelium-independent non-vesicular, non-quantal ACh release in the mouse trachea involving low-affinity choline transporters. PMID:26278668

  11. Remote Asynchronous Message Service Gateway

    NASA Technical Reports Server (NTRS)

    Wang, Shin-Ywan; Burleigh, Scott C.

    2011-01-01

    The Remote Asynchronous Message Service (RAMS) gateway is a special-purpose AMS application node that enables exchange of AMS messages between nodes residing in different AMS "continua," notionally in different geographical locations. JPL s implementation of RAMS gateway functionality is integrated with the ION (Interplanetary Overlay Network) implementation of the DTN (Delay-Tolerant Networking) bundle protocol, and with JPL s implementation of AMS itself. RAMS protocol data units are encapsulated in ION bundles and are forwarded to the neighboring RAMS gateways identified in the source gateway s AMS management information base. Each RAMS gateway has interfaces in two communication environments: the AMS message space it serves, and the RAMS network - the grid or tree of mutually aware RAMS gateways - that enables AMS messages produced in one message space to be forwarded to other message spaces of the same venture. Each gateway opens persistent, private RAMS network communication channels to the RAMS gateways of other message spaces for the same venture, in other continua. The interconnected RAMS gateways use these communication channels to forward message petition assertions and cancellations among themselves. Each RAMS gateway subscribes locally to all subjects that are of interest in any of the linked message spaces. On receiving its copy of a message on any of these subjects, the RAMS gateway node uses the RAMS network to forward the message to every other RAMS gateway whose message space contains at least one node that has subscribed to messages on that subject. On receiving a message via the RAMS network from some other RAMS gateway, the RAMS gateway node forwards the message to all subscribers in its own message space.

  12. Asynchronous vibration problem of centrifugal compressor

    NASA Technical Reports Server (NTRS)

    Fujikawa, T.; Ishiguro, N.; Ito, M.

    1980-01-01

    An unstable asynchronous vibration problem in a high pressure centrifugal compressor and the remedial actions against it are described. Asynchronous vibration of the compressor took place when the discharge pressure (Pd) was increased, after the rotor was already at full speed. The typical spectral data of the shaft vibration indicate that as the pressure Pd increases, pre-unstable vibration appears and becomes larger, and large unstable asynchronous vibration occurs suddenly (Pd = 5.49MPa). A computer program was used which calculated the logarithmic decrement and the damped natural frequency of the rotor bearing systems. The analysis of the log-decrement is concluded to be effective in preventing unstable vibration in both the design stage and remedial actions.

  13. Photon detection with parallel asynchronous processing

    NASA Technical Reports Server (NTRS)

    Coon, D. D.; Perera, A. G. U.

    1990-01-01

    An approach to photon detection with a parallel asynchronous signal processor is described. The visible or IR photon-detection capability of the silicon p(+)-n-n(+) detectors and the parallel asynchronous processing are addressed separately. This approach would permit an independent analog processing channel to be dedicated to every pixel. A laminar architecture consisting of a stack of planar arrays of the devices would form a 2D array processor with a 2D array of inputs located directly behind a focal-plane detector array. A 2D image data stream would propagate in neuronlike asynchronous pulse-coded form through the laminar processor. Such systems can integrate image acquisition and image processing. Acquisition and processing would be performed concurrently as in natural vision systems. The possibility of multispectral image processing is addressed.

  14. Asynchronous Message Service Reference Implementation

    NASA Technical Reports Server (NTRS)

    Burleigh, Scott C.

    2011-01-01

    This software provides a library of middleware functions with a simple application programming interface, enabling implementation of distributed applications in conformance with the CCSDS AMS (Consultative Committee for Space Data Systems Asynchronous Message Service) specification. The AMS service, and its protocols, implement an architectural concept under which the modules of mission systems may be designed as if they were to operate in isolation, each one producing and consuming mission information without explicit awareness of which other modules are currently operating. Communication relationships among such modules are self-configuring; this tends to minimize complexity in the development and operations of modular data systems. A system built on this model is a society of generally autonomous, inter-operating modules that may fluctuate freely over time in response to changing mission objectives, modules functional upgrades, and recovery from individual module failure. The purpose of AMS, then, is to reduce mission cost and risk by providing standard, reusable infrastructure for the exchange of information among data system modules in a manner that is simple to use, highly automated, flexible, robust, scalable, and efficient. The implementation is designed to spawn multiple threads of AMS functionality under the control of an AMS application program. These threads enable all members of an AMS-based, distributed application to discover one another in real time, subscribe to messages on specific topics, and to publish messages on specific topics. The query/reply (client/server) communication model is also supported. Message exchange is optionally subject to encryption (to support confidentiality) and authorization. Fault tolerance measures in the discovery protocol minimize the likelihood of overall application failure due to any single operational error anywhere in the system. The multi-threaded design simplifies processing while enabling application nodes to

  15. Loss of Local Astrocyte Support Disrupts Action Potential Propagation and Glutamate Release Synchrony from Unmyelinated Hippocampal Axon Terminals In Vitro

    PubMed Central

    Sobieski, Courtney; Jiang, Xiaoping; Crawford, Devon C.

    2015-01-01

    Neuron–astrocyte interactions are critical for proper CNS development and function. Astrocytes secrete factors that are pivotal for synaptic development and function, neuronal metabolism, and neuronal survival. Our understanding of this relationship, however, remains incomplete due to technical hurdles that have prevented the removal of astrocytes from neuronal circuits without changing other important conditions. Here we overcame this obstacle by growing solitary rat hippocampal neurons on microcultures that were comprised of either an astrocyte bed (+astrocyte) or a collagen bed (−astrocyte) within the same culture dish. −Astrocyte autaptic evoked EPSCs, but not IPSCs, displayed an altered temporal profile, which included increased synaptic delay, increased time to peak, and severe glutamate release asynchrony, distinct from previously described quantal asynchrony. Although we observed minimal alteration of the somatically recorded action potential waveform, action potential propagation was altered. We observed a longer latency between somatic initiation and arrival at distal locations, which likely explains asynchronous EPSC peaks, and we observed broadening of the axonal spike, which likely underlies changes to evoked EPSC onset. No apparent changes in axon structure were observed, suggesting altered axonal excitability. In conclusion, we propose that local astrocyte support has an unappreciated role in maintaining glutamate release synchrony by disturbing axonal signal propagation. SIGNIFICANCE STATEMENT Certain glial cell types (oligodendrocytes, Schwann cells) facilitate the propagation of neuronal electrical signals, but a role for astrocytes has not been identified despite many other functions of astrocytes in supporting and modulating neuronal signaling. Under identical global conditions, we cultured neurons with or without local astrocyte support. Without local astrocytes, glutamate transmission was desynchronized by an alteration of the waveform

  16. Release properties of individual presynaptic boutons expressed during homosynaptic depression and heterosynaptic facilitation of the Aplysia sensorimotor synapse

    PubMed Central

    Malkinson, Guy; Spira, Micha E.

    2013-01-01

    Much of what we know about the mechanisms underlying Homosynaptic Depression (HSD) and heterosynaptic facilitation is based on intracellular recordings of integrated postsynaptic potentials (PSPs). This methodological approach views the presynaptic apparatus as a single compartment rather than taking a more realistic representation reflecting the fact that it is made up of tens to hundreds of individual and independent Presynaptic Release Boutons (PRBs). Using cultured Aplysia sensorimotor synapses, we reexamined HSD and its dishabituation by imaging the release properties of individual PRBs. We find that the PRB population is heterogeneous and can be clustered into three groups: ~25% of the PRBs consistently release neurotransmitter throughout the entire habituation paradigm (35 stimuli, 0.05 Hz) and have a relatively high quantal content, 36% of the PRBs display intermittent failures only after the tenth stimulation, and 39% are low quantal-content PRBs that exhibit intermittent release failures from the onset of the habituation paradigm. 5HT-induced synaptic dishabituation by a single 5HT application was generated by the enhanced recovery of the quantal content of the habituated PRBs and did not involve the recruitment of new release boutons. The characterization of the PRB population as heterogeneous in terms of its temporal pattern of release-probability and quantal content provides new insights into the mechanisms underlying HSD and its dishabituation. PMID:24068986

  17. Release properties of individual presynaptic boutons expressed during homosynaptic depression and heterosynaptic facilitation of the Aplysia sensorimotor synapse.

    PubMed

    Malkinson, Guy; Spira, Micha E

    2013-01-01

    Much of what we know about the mechanisms underlying Homosynaptic Depression (HSD) and heterosynaptic facilitation is based on intracellular recordings of integrated postsynaptic potentials (PSPs). This methodological approach views the presynaptic apparatus as a single compartment rather than taking a more realistic representation reflecting the fact that it is made up of tens to hundreds of individual and independent Presynaptic Release Boutons (PRBs). Using cultured Aplysia sensorimotor synapses, we reexamined HSD and its dishabituation by imaging the release properties of individual PRBs. We find that the PRB population is heterogeneous and can be clustered into three groups: ~25% of the PRBs consistently release neurotransmitter throughout the entire habituation paradigm (35 stimuli, 0.05 Hz) and have a relatively high quantal content, 36% of the PRBs display intermittent failures only after the tenth stimulation, and 39% are low quantal-content PRBs that exhibit intermittent release failures from the onset of the habituation paradigm. 5HT-induced synaptic dishabituation by a single 5HT application was generated by the enhanced recovery of the quantal content of the habituated PRBs and did not involve the recruitment of new release boutons. The characterization of the PRB population as heterogeneous in terms of its temporal pattern of release-probability and quantal content provides new insights into the mechanisms underlying HSD and its dishabituation. PMID:24068986

  18. Implications of Tracey's theorem to asynchronous sequential circuit design

    NASA Technical Reports Server (NTRS)

    Gopalakrishnan, S.; Kim, G.; Maki, G.

    1990-01-01

    Tracey's Theorem has long been recognized as essential in generating state assignments for asynchronous sequential circuits. This paper shows that Tracey's Theorem also has a significant impact in generating the design equations. Moreover, this theorem is important to the fundamental understanding of asynchronous sequential operation. The results of this work simplify asynchronous logic design. Moreover, detection of safe circuits is made easier.

  19. Acceptability of an Asynchronous Learning Forum on Mobile Devices

    ERIC Educational Resources Information Center

    Chang, Chih-Kai

    2010-01-01

    Mobile learning has recently become noteworthy because mobile devices have become popular. To construct an asynchronous learning forum on mobile devices is important because an asynchronous learning forum is always an essential part of networked asynchronous distance learning. However, the input interface in handheld learning devices, which is…

  20. Increasing Student Engagement Using Asynchronous Learning

    ERIC Educational Resources Information Center

    Northey, Gavin; Bucic, Tania; Chylinski, Mathew; Govind, Rahul

    2015-01-01

    Student engagement is an ongoing concern for educators because of its positive association with deep learning and educational outcomes. This article tests the use of a social networking site (Facebook) as a tool to facilitate asynchronous learning opportunities that complement face-to-face interactions and thereby enable a stronger learning…

  1. Interaction and Cognition in Asynchronous Computer Conferencing

    ERIC Educational Resources Information Center

    Schrire, Sarah

    2004-01-01

    This paper is based on a multiple-case study of the learning process in three asynchronous computer conferences. The conferences were part of the distance learning component in doctoral degree courses in computing technology in education offered at an American university. The conferences were analyzed from a number of perspectives, the emphasis in…

  2. FIFO Buffer for Asynchronous Data Streams

    NASA Technical Reports Server (NTRS)

    Bascle, K. P.

    1985-01-01

    Variable-rate, asynchronous data signals from up to four measuring instruments or other sources combined in first-in/first-out (FIFO) buffer for transmission on single channel. Constructed in complementary metal-oxide-semiconductor (CMOS) logic, buffer consumes low power (only 125 mW at 5V) and conforms to aerospace standards of reliability and maintainability.

  3. Blending Online Asynchronous and Synchronous Learning

    ERIC Educational Resources Information Center

    Yamagata-Lynch, Lisa C.

    2014-01-01

    In this article I will share a qualitative self-study about a 15-week blended 100% online graduate level course facilitated through synchronous meetings on Blackboard Collaborate and asynchronous discussions on Blackboard. I taught the course at the University of Tennessee (UT) during the spring 2012 semester and the course topic was online…

  4. An Agent Architecture for Asynchronous Learning.

    ERIC Educational Resources Information Center

    Rochefort, Stephen

    Education delivery systems have evolved from content delivery mechanisms to collaborative environments. The next step in this evolution is to provide greater support for asynchronous learners, those that progress through course material with little or no support from the instructional team. The integration of Web technologies with artificial…

  5. Asynchronous Learning in Manufacturing: Review of Research.

    ERIC Educational Resources Information Center

    Bundy, Gail E.

    This research paper examines the potential for asynchronous learning networks as a viable training alternative for small and medium manufacturing businesses. This first phase of the project, conducted by the Colorado Community College and Occupational System and the National Association of Manufacturers reviews existing research with a specific…

  6. Asynchronous file transfer to IBM PC's

    NASA Technical Reports Server (NTRS)

    Hoerger, J.

    1984-01-01

    The Asynchronous File Transfer System is used for interactively selecting and tramsmitting text files from the Langley Research Center's Business Data Systems Division (BDSD) host processor to an IBM Personal Computer. The IBM asynchronous communications support package is used for handling the communications on the personal computer. An application program (NATURAL, COBOL, etc.) is used for selecting and formatting the records to be transmitted, and three subroutine modules residing on the BDSD host processor are used for interfacing the application program and the communications software. Each record transmitted to the Personal Computer must be in the standard ASCII format. This record format is directly accessible by BASIC and many of the personal computer software packages provide utility programs for converting them to the format required for the particular package in question.

  7. Inhibition Controls Asynchronous States of Neuronal Networks

    PubMed Central

    Treviño, Mario

    2016-01-01

    Computations in cortical circuits require action potentials from excitatory and inhibitory neurons. In this mini-review, I first provide a quick overview of findings that indicate that GABAergic neurons play a fundamental role in coordinating spikes and generating synchronized network activity. Next, I argue that these observations helped popularize the notion that network oscillations require a high degree of spike correlations among interneurons which, in turn, produce synchronous inhibition of the local microcircuit. The aim of this text is to discuss some recent experimental and computational findings that support a complementary view: one in which interneurons participate actively in producing asynchronous states in cortical networks. This requires a proper mixture of shared excitation and inhibition leading to asynchronous activity between neighboring cells. Such contribution from interneurons would be extremely important because it would tend to reduce the spike correlation between neighboring pyramidal cells, a drop in redundancy that could enhance the information-processing capacity of neural networks. PMID:27274721

  8. Asynchronous Communication Scheme For Hypercube Computer

    NASA Technical Reports Server (NTRS)

    Madan, Herb S.

    1988-01-01

    Scheme devised for asynchronous-message communication system for Mark III hypercube concurrent-processor network. Network consists of up to 1,024 processing elements connected electrically as though were at corners of 10-dimensional cube. Each node contains two Motorola 68020 processors along with Motorola 68881 floating-point processor utilizing up to 4 megabytes of shared dynamic random-access memory. Scheme intended to support applications requiring passage of both polled or solicited and unsolicited messages.

  9. Asynchronous event-based binocular stereo matching.

    PubMed

    Rogister, Paul; Benosman, Ryad; Ieng, Sio-Hoi; Lichtsteiner, Patrick; Delbruck, Tobi

    2012-02-01

    We present a novel event-based stereo matching algorithm that exploits the asynchronous visual events from a pair of silicon retinas. Unlike conventional frame-based cameras, recent artificial retinas transmit their outputs as a continuous stream of asynchronous temporal events, in a manner similar to the output cells of the biological retina. Our algorithm uses the timing information carried by this representation in addressing the stereo-matching problem on moving objects. Using the high temporal resolution of the acquired data stream for the dynamic vision sensor, we show that matching on the timing of the visual events provides a new solution to the real-time computation of 3-D objects when combined with geometric constraints using the distance to the epipolar lines. The proposed algorithm is able to filter out incorrect matches and to accurately reconstruct the depth of moving objects despite the low spatial resolution of the sensor. This brief sets up the principles for further event-based vision processing and demonstrates the importance of dynamic information and spike timing in processing asynchronous streams of visual events. PMID:24808513

  10. Enabling techniques for asynchronous coherent OCDMA

    NASA Astrophysics Data System (ADS)

    Wang, Xu; Wada, Naoya; Kitayama, Ken-ichi

    2005-11-01

    The coherent OCDMA system could suffer from severe multiple access interference (MAI) and beat noise, which limit the maximum number of active users that can be supported in a network. One effective method to reduce the beat noise as well as the MAI noise is to lower the interference level by adopting ultra-long optical code. Applying optical thresholding technique is also crucial to enable data-rate detection for achieving a practical OCDMA system. In this paper, we review the recent progress in the key enabling techniques for asynchronous coherent OCDMA: the novel encoder/decoders including spatial lightwave phase modulator, micro-ring resonator for spectral phase coding and superstructured FBG (SSFBG) and AWG type encode/decoder for time-spreading coding; optical thresholding techniques with PPLN and nonlinearity in fiber. The FEC has also been applied in OCDMA system recently. With 511-chip SSFBG and SC-based optical thresholder, 10-user, truly-asynchronous gigabit OCDMA transmission has been successfully achieved. Most recently, a record throughput 12×10.71 Gbps truly-asynchronous OCDMA has been demonstrated by using the 16×16 ports AWG-type encoder/decoder and FEC transmit ITU-T G.709 OTN frames.

  11. Automated Maximum Likelihood Separation of Signal from Baseline in Noisy Quantal Data

    PubMed Central

    Bruno, William J.; Ullah, Ghanim; Daniel Mak, Don-On; Pearson, John E.

    2013-01-01

    Data recordings often include high-frequency noise and baseline fluctuations that are not generated by the system under investigation, which need to be removed before analyzing the signal for the system’s behavior. In the absence of an automated method, experimentalists fall back on manual procedures for removing these fluctuations, which can be laborious and prone to subjective bias. We introduce a maximum likelihood formalism for separating signal from a drifting baseline plus noise, when the signal takes on integer multiples of some value, as in ion channel patch-clamp current traces. Parameters such as the quantal step size (e.g., current passing through a single channel), noise amplitude, and baseline drift rate can all be optimized automatically using the expectation-maximization algorithm, taking the number of open channels (or molecules in the on-state) at each time point as a hidden variable. Our goal here is to reconstruct the signal, not model the (possibly highly complex) underlying system dynamics. Thus, our likelihood function is independent of those dynamics. This may be thought of as restricting to the simplest possible hidden Markov model for the underlying channel current, in which successive measurements of the state of the channel(s) are independent. The resulting method is comparable to an experienced human in terms of results, but much faster. FORTRAN 90, C, R, and JAVA codes that implement the algorithm are available for download from our website. PMID:23823225

  12. Automated maximum likelihood separation of signal from baseline in noisy quantal data.

    PubMed

    Bruno, William J; Ullah, Ghanim; Mak, Don-On Daniel; Pearson, John E

    2013-07-01

    Data recordings often include high-frequency noise and baseline fluctuations that are not generated by the system under investigation, which need to be removed before analyzing the signal for the system's behavior. In the absence of an automated method, experimentalists fall back on manual procedures for removing these fluctuations, which can be laborious and prone to subjective bias. We introduce a maximum likelihood formalism for separating signal from a drifting baseline plus noise, when the signal takes on integer multiples of some value, as in ion channel patch-clamp current traces. Parameters such as the quantal step size (e.g., current passing through a single channel), noise amplitude, and baseline drift rate can all be optimized automatically using the expectation-maximization algorithm, taking the number of open channels (or molecules in the on-state) at each time point as a hidden variable. Our goal here is to reconstruct the signal, not model the (possibly highly complex) underlying system dynamics. Thus, our likelihood function is independent of those dynamics. This may be thought of as restricting to the simplest possible hidden Markov model for the underlying channel current, in which successive measurements of the state of the channel(s) are independent. The resulting method is comparable to an experienced human in terms of results, but much faster. FORTRAN 90, C, R, and JAVA codes that implement the algorithm are available for download from our website. PMID:23823225

  13. The analysis of dose-response curve from bioassays with quantal response: Deterministic or statistical approaches?

    PubMed

    Mougabure-Cueto, G; Sfara, V

    2016-04-25

    Dose-response relations can be obtained from systems at any structural level of biological matter, from the molecular to the organismic level. There are two types of approaches for analyzing dose-response curves: a deterministic approach, based on the law of mass action, and a statistical approach, based on the assumed probabilities distribution of phenotypic characters. Models based on the law of mass action have been proposed to analyze dose-response relations across the entire range of biological systems. The purpose of this paper is to discuss the principles that determine the dose-response relations. Dose-response curves of simple systems are the result of chemical interactions between reacting molecules, and therefore are supported by the law of mass action. In consequence, the shape of these curves is perfectly sustained by physicochemical features. However, dose-response curves of bioassays with quantal response are not explained by the simple collision of molecules but by phenotypic variations among individuals and can be interpreted as individual tolerances. The expression of tolerance is the result of many genetic and environmental factors and thus can be considered a random variable. In consequence, the shape of its associated dose-response curve has no physicochemical bearings; instead, they are originated from random biological variations. Due to the randomness of tolerance there is no reason to use deterministic equations for its analysis; on the contrary, statistical models are the appropriate tools for analyzing these dose-response relations. PMID:26952004

  14. How to build fast muscles: synchronous and asynchronous designs.

    PubMed

    Syme, Douglas A; Josephson, Robert K

    2002-08-01

    In animals, muscles are the most common effectors that translate neuronal activity into behavior. Nowhere is behavior more restricted by the limits of muscle performance than at the upper range of high-frequency movements. Here, we see new and multiple designs to cope with the demands for speed. Extremely rapid oscillations in force are required to power cyclic activities such as flight in insects or to produce vibrations for sound. Such behaviors are seen in a variety of invertebrates and vertebrates, and are powered by both synchronous and asynchronous muscles. In synchronous muscles, each contraction/relaxation cycle is accompanied by membrane depolarization and subsequent repolarization, release of activator calcium, attachment of cross-bridges and muscle shortening, then removal of activator calcium and cross-bridge detachment. To enable all of these to occur at extremely high frequencies a suite of modifications are required, including precise neural control, hypertrophy of the calcium handling machinery, innovative mechanisms to bind calcium, and molecular modification of the cross-bridges and regulatory proteins. Side effects are low force and power output and low efficiency, but the benefit of direct, neural control is maintained. Asynchronous muscles, in which there is not a 1:1 correspondence between neural activation and contraction, are a radically different design. Rather than rapid calcium cycling, they rely on delayed activation and deactivation, and the resonant characteristics of the wings and exoskeleton to guide their extremely high-frequency contractions. They thus avoid many of the modifications and attendant trade-offs mentioned above, are more powerful and more efficient than high-frequency synchronous muscles, but are considerably more restricted in their application. PMID:21708773

  15. Facilitating Asynchronous Distance Learning: Exploiting Opportunities for Knowledge Building in Asynchronous Distance Learning Environments.

    ERIC Educational Resources Information Center

    Prestera, Gustavo E.; Moller, Leslie A.

    Computer mediated communication tools enable today's distance learners to engage in collaborative problem solving, threaded discussions, and peer tutoring through asynchronous distance learning environments. This paper suggests that these are best accomplished by establishing virtual learning communities, which break down traditional…

  16. DAI-CLIPS: Distributed, Asynchronous, Interacting CLIPS

    NASA Technical Reports Server (NTRS)

    Gagne, Denis; Garant, Alain

    1994-01-01

    DAI-CLIPS is a distributed computational environment within which each CLIPS is an active independent computational entity with the ability to communicate freely with other CLIPS. Furthermore, new CLIPS can be created, others can be deleted or modify their expertise, all dynamically in an asynchronous and independent fashion during execution. The participating CLIPS are distributed over a network of heterogeneous processors taking full advantage of the available processing power. We present the general framework encompassing DAI-CLIPS and discuss some of its advantages and potential applications.

  17. Pass-transistor asynchronous sequential circuits

    NASA Astrophysics Data System (ADS)

    Whitaker, Sterling R.; Maki, Gary K.

    1989-02-01

    Design methods for asynchronous sequential pass-transistor circuits, which result in circuits that are hazard- and critical-race-free and which have added degrees of freedom for the input signals, are discussed. The design procedures are straightforward and easy to implement. Two single-transition-time state assignment methods are presented, and hardware bounds for each are established. A surprising result is that the hardware realizations for each next state variable and output variable is identical for a given flow table. Thus, a state machine with N states and M outputs can be constructed using a single layout replicated N + M times.

  18. Asynchronous Event-Driven Particle Algorithms

    SciTech Connect

    Donev, A

    2007-08-30

    We present, in a unifying way, the main components of three asynchronous event-driven algorithms for simulating physical systems of interacting particles. The first example, hard-particle molecular dynamics (MD), is well-known. We also present a recently-developed diffusion kinetic Monte Carlo (DKMC) algorithm, as well as a novel stochastic molecular-dynamics algorithm that builds on the Direct Simulation Monte Carlo (DSMC). We explain how to effectively combine event-driven and classical time-driven handling, and discuss some promises and challenges for event-driven simulation of realistic physical systems.

  19. Asynchronous Event-Driven Particle Algorithms

    SciTech Connect

    Donev, A

    2007-02-28

    We present in a unifying way the main components of three examples of asynchronous event-driven algorithms for simulating physical systems of interacting particles. The first example, hard-particle molecular dynamics (MD), is well-known. We also present a recently-developed diffusion kinetic Monte Carlo (DKMC) algorithm, as well as a novel event-driven algorithm for Direct Simulation Monte Carlo (DSMC). Finally, we describe how to combine MD with DSMC in an event-driven framework, and discuss some promises and challenges for event-driven simulation of realistic physical systems.

  20. Assessment of Alternate Delivery Mechanisms for Asynchronous Adult Distance Learners.

    ERIC Educational Resources Information Center

    Usrey, Michael W.

    Within a course at the University of Colorado addressing the management of research and development, a comparison of alternate asynchronous delivery media was conducted. For the course session on the management of intellectual property, asynchronous students were given access to the VHS tapes traditionally utilized, plus World Wide Web-based and…

  1. Interactional Coherence in Asynchronous Learning Networks: A Rhetorical Approach

    ERIC Educational Resources Information Center

    Potter, Andrew

    2008-01-01

    Numerous studies have affirmed the value of asynchronous online communication as a learning resource. Several investigations, however, have indicated that discussions in asynchronous environments are often neither interactive nor coherent. The research reported sought to develop an enhanced understanding of interactional coherence, argumentation,…

  2. Teaching Presence and Communication Timeliness in Asynchronous Online Courses

    ERIC Educational Resources Information Center

    Skramstad, Erik; Schlosser, Charles; Orellana, Anymir

    2012-01-01

    This study examined student perceptions of teaching presence and communication timeliness in asynchronous online courses. Garrison, Anderson, and Archer's (2000) community of inquiry model provided the framework for the survey research methodology used. Participants were 59 student volunteers taking 1 or more asynchronous online graduate courses.…

  3. Asynchronous Learning Sources in a High-Tech Organization

    ERIC Educational Resources Information Center

    Bouhnik, Dan; Giat, Yahel; Sanderovitch, Yafit

    2009-01-01

    Purpose: The purpose of this study is to characterize learning from asynchronous sources among research and development (R&D) personnel. It aims to examine four aspects of asynchronous source learning: employee preferences regarding self-learning; extent of source usage; employee satisfaction with these sources and the effect of the sources on the…

  4. Localized radio frequency communication using asynchronous transfer mode protocol

    DOEpatents

    Witzke, Edward L.; Robertson, Perry J.; Pierson, Lyndon G.

    2007-08-14

    A localized wireless communication system for communication between a plurality of circuit boards, and between electronic components on the circuit boards. Transceivers are located on each circuit board and electronic component. The transceivers communicate with one another over spread spectrum radio frequencies. An asynchronous transfer mode protocol controls communication flow with asynchronous transfer mode switches located on the circuit boards.

  5. Using Asynchronous Online Discussions in Primary School Project Work

    ERIC Educational Resources Information Center

    Jamaludin, Azilawati; Lang, Quek Choon

    2006-01-01

    Using asynchronous online discussions for interschool collaborative project work represents one of the innovative practices in the Singapore classroom. With anytime, anywhere access to interactions among the students and teachers, the asynchronous nature of these interactions leads to new paradigms for teaching and learning, with both unique…

  6. Asynchronous Access to Conventional Course Delivery: A Pilot Project

    ERIC Educational Resources Information Center

    Simpson, Natalie

    2006-01-01

    This study describes a technological pilot project providing 160 graduate students with asynchronous access to the ongoing proceedings of a lecture-based course. Exactly half of the students spoke the language of instruction, English, as a second language (ESL). While the asynchronous video-on-demand service proved popular overall, ESL students…

  7. Integrating Asynchronous Digital Design Into the Computer Engineering Curriculum

    ERIC Educational Resources Information Center

    Smith, S. C.; Al-Assadi, W. K.; Di, J.

    2010-01-01

    As demand increases for circuits with higher performance, higher complexity, and decreased feature size, asynchronous (clockless) paradigms will become more widely used in the semiconductor industry, as evidenced by the International Technology Roadmap for Semiconductors' (ITRS) prediction of a likely shift from synchronous to asynchronous design…

  8. Exploring Asynchronous and Synchronous Tool Use in Online Courses

    ERIC Educational Resources Information Center

    Oztok, Murat; Zingaro, Daniel; Brett, Clare; Hewitt, Jim

    2013-01-01

    While the independent contributions of synchronous and asynchronous interaction in online learning are clear, comparatively less is known about the pedagogical consequences of using both modes in the same environment. In this study, we examine relationships between students' use of asynchronous discussion forums and synchronous private messages…

  9. Asynchronicity of Facial Blood Perfusion in Migraine

    PubMed Central

    Zaproudina, Nina; Teplov, Victor; Nippolainen, Ervin; Lipponen, Jukka A.; Kamshilin, Alexei A.; Närhi, Matti; Karjalainen, Pasi A.; Giniatullin, Rashid

    2013-01-01

    Asymmetrical changes in blood perfusion and asynchronous blood supply to head tissues likely contribute to migraine pathophysiology. Imaging was widely used in order to understand hemodynamic variations in migraine. However, mapping of blood pulsations in the face of migraineurs has not been performed so far. We used the Blood Pulsation Imaging (BPI) technique, which was recently developed in our group, to establish whether 2D-imaging of blood pulsations parameters can reveal new biomarkers of migraine. BPI characteristics were measured in migraineurs during the attack-free interval and compared to healthy subjects with and without a family history of migraine. We found a novel phenomenon of transverse waves of facial blood perfusion in migraineurs in contrast to healthy subjects who showed synchronous blood delivery to both sides of the face. Moreover, the amplitude of blood pulsations was symmetrically distributed over the face of healthy subjects, but asymmetrically in migraineurs and subjects with a family history of migraine. In the migraine patients we found a remarkable correlation between the side of unilateral headache and the direction of the blood perfusion wave. Our data suggest that migraine is associated with lateralization of blood perfusion and asynchronous blood pulsations in the facial area, which could be due to essential dysfunction of the autonomic vascular control in the face. These findings may further enhance our understanding of migraine pathophysiology and suggest new easily available biomarkers of this pathology. PMID:24324592

  10. Purifying Cytokinetic Cells from an Asynchronous Population

    PubMed Central

    Panet, Einat; Ozer, Efrat; Mashriki, Tal; Lazar, Itay; Itzkovich, Devora; Tzur, Amit

    2015-01-01

    Cytokinesis is an intensively studied process by which the cell cytoplasm divides to produce two daughter cells. Like any other aspect of cell cycle research, the study of cytokinesis relies heavily on cell synchronization. However, the synchronization of cells during cytokinesis is challenging due to the rapid nature of this process and the shortage of cell cycle blocking agents specifically targeting this phase. Here, we demonstrate the use of standard flow cytometry for directly isolating cytokinetic cells from an asynchronous population of normally proliferating cells. This approach is based on a cell cycle marker whose temporal proteolysis, in combination with DNA quantification or cell size approximation, distinguishes cells undergoing cytokinesis. Furthermore, by avoiding doublet discrimination, typically used in flow cytometry analyses, we were able to further increase selectivity, specifically purifying cells at late cytokinesis. Our method circumvents checkpoint activation, cell cycle arrest, and any other means of pre-synchronization. These qualities, as demonstrated for both unattached and adherent cells, enable high selectivity for cytokinetic cells despite their overall low abundance in an asynchronous population. The sorted cells can then be readily used for cell biological, biochemical, and genomic applications to facilitate cytokinesis and cell cycle research. PMID:26260981

  11. Controllability of asynchronous Boolean multiplex control networks

    NASA Astrophysics Data System (ADS)

    Luo, Chao; Wang, Xingyuan; Liu, Hong

    2014-09-01

    In this article, the controllability of asynchronous Boolean multiplex control networks (ABMCNs) is studied. First, the model of Boolean multiplex control networks under Harvey' asynchronous update is presented. By means of semi-tensor product approach, the logical dynamics is converted into linear representation, and a generalized formula of control-depending network transition matrices is achieved. Second, a necessary and sufficient condition is proposed to verify that only control-depending fixed points of ABMCNs can be controlled with probability one. Third, using two types of controls, the controllability of system is studied and formulae are given to show: (a) when an initial state is given, the reachable set at time s under a group of specified controls; (b) the reachable set at time s under arbitrary controls; (c) the specific probability values from a given initial state to destination states. Based on the above formulae, an algorithm to calculate overall reachable states from a specified initial state is presented. Moreover, we also discuss an approach to find the particular control sequence which steers the system between two states with maximum probability. Examples are shown to illustrate the feasibility of the proposed scheme.

  12. Time-coded neurotransmitter release at excitatory and inhibitory synapses

    PubMed Central

    Rodrigues, Serafim; Desroches, Mathieu; Krupa, Martin; Cortes, Jesus M.; Sejnowski, Terrence J.; Ali, Afia B.

    2016-01-01

    Communication between neurons at chemical synapses is regulated by hundreds of different proteins that control the release of neurotransmitter that is packaged in vesicles, transported to an active zone, and released when an input spike occurs. Neurotransmitter can also be released asynchronously, that is, after a delay following the spike, or spontaneously in the absence of a stimulus. The mechanisms underlying asynchronous and spontaneous neurotransmitter release remain elusive. Here, we describe a model of the exocytotic cycle of vesicles at excitatory and inhibitory synapses that accounts for all modes of vesicle release as well as short-term synaptic plasticity (STSP). For asynchronous release, the model predicts a delayed inertial protein unbinding associated with the SNARE complex assembly immediately after vesicle priming. Experiments are proposed to test the model’s molecular predictions for differential exocytosis. The simplicity of the model will also facilitate large-scale simulations of neural circuits. PMID:26858411

  13. Time-coded neurotransmitter release at excitatory and inhibitory synapses.

    PubMed

    Rodrigues, Serafim; Desroches, Mathieu; Krupa, Martin; Cortes, Jesus M; Sejnowski, Terrence J; Ali, Afia B

    2016-02-23

    Communication between neurons at chemical synapses is regulated by hundreds of different proteins that control the release of neurotransmitter that is packaged in vesicles, transported to an active zone, and released when an input spike occurs. Neurotransmitter can also be released asynchronously, that is, after a delay following the spike, or spontaneously in the absence of a stimulus. The mechanisms underlying asynchronous and spontaneous neurotransmitter release remain elusive. Here, we describe a model of the exocytotic cycle of vesicles at excitatory and inhibitory synapses that accounts for all modes of vesicle release as well as short-term synaptic plasticity (STSP). For asynchronous release, the model predicts a delayed inertial protein unbinding associated with the SNARE complex assembly immediately after vesicle priming. Experiments are proposed to test the model's molecular predictions for differential exocytosis. The simplicity of the model will also facilitate large-scale simulations of neural circuits. PMID:26858411

  14. First-principles molecular dynamics of liquid alkali metals based on the quantal hypernetted chain theory

    NASA Astrophysics Data System (ADS)

    Kambayashi, Shaw; Chihara, Junzo

    1996-06-01

    A first-principles molecular dynamics (MD) scheme is presented on the basis of the density-functional (DF) theory with use of the the quantal hypernetted chain (QHNC) approximation. The DF theory brings about exact expressions for the ion-electron and ion-ion radial distribution functions (RDF) of an electron-ion mixture as a model of a simple liquid metal. These exact expressions prove that an ion-electron mixture can be treated as a one-component liquid interacting only via a pairwise interaction in the evaluation of the ion-ion RDF, and provide a set of integral equations: one is an exact integral equation for the ion-ion RDF and another for an effective ion-ion interaction, which depends on the ion configuration specified by the ion-ion RDF. Hence, after some approximations are introduced, the MD simulation can be performed to get the ion-ion RDF using the ion-ion interaction determined so as to be consistent to the ion-ion RDF: the MD simulation and the procedure to determine the effective interaction from the QHNC equation are performed iteratively. This MD simulation coupled with the QHNC equation (QHNC-MD method) for the effective interaction provides a first-principles calculation of structures of simple liquid metal: the ion-ion and electron-ion RDF's, the charge distributions of an ion and a pseudoatom, the effective ion-ion interaction and the ion-ion bridge function are evaluated in a self-consistent manner from the atomic number as the only input. We have applied this QHNC-MD method to Li, Na, K, Rb, and Cs near the melting temperature using up to 16 000 particles for the MD simulation. It is found that the convergence of the effective ion-ion interaction is fast enough for practical application to alkali metals; two MD runs are enough for convergence within accuracy of 3 to 4 digits, if the initial effective potential is properly set up. The structure factors, thus obtained, show excellent agreement with the experimental data observed by x-ray and

  15. PDE type-4 inhibition increases L-type Ca(2+) currents, action potential firing, and quantal size of exocytosis in mouse chromaffin cells.

    PubMed

    Marcantoni, A; Carabelli, V; Vandael, D H; Comunanza, V; Carbone, E

    2009-03-01

    We studied the effects of the cAMP-hydrolyzing enzyme phosphodiesterase type-4 (PDE4) on the L-type Ca(2+) channels (LTCCs) and Ca(2+)-dependent secretion in mouse chromaffin cells (MCCs). The selective PDE4 inhibitor rolipram (3 microM) had a specific potentiating action on Ca(2+) currents of MCCs (40% increase within 3 min). A similar effect was produced by the selective beta(1)-AR agonist denopamine (1 microM) and by the unselective PDEs inhibitor IBMX (100 microM). Rolipram and denopamine actions were selective for LTCCs, and the Ca(2+) current increase remained unchanged if the two compounds were applied simultaneously. This suggests that at rest, LTCCs in MCCs are down-regulated by the low levels of cAMP determined by PDE4 activity and that LTCCs can be up-regulated by either inhibiting PDE4 or activating beta(1)-AR. No other PDEs are likely involved in this specific action. PDE4 inhibition had also a marked effect on the spontaneous firing of resting MCCs and catecholamine secretion. Rolipram up-regulated the LTCCs contributing to the "pace-maker" current underlying action potential (AP) discharges and accelerated the firing rate, with no significant effects on AP waveform. Acceleration of AP firing was also induced by the LTCC-agonist Bay K (1 microM), while nifedipine (3 microM) reduced the firing frequency, suggesting that LTCCs and intracellular cAMP play a key role in setting the pace-maker current regulating MCCs excitability. Rolipram increased also the size of the ready-releasable pool and the quantal content of secretory vesicles without affecting their probability of release. Thus, rolipram acts on MCCs by up-regulating both exocytosis and AP firings. These two processes are effectively down-regulated by PDE4 at rest and can dramatically increase the quantity of released catecholamines when PDE4 is inhibited and/or cAMP is raised. PMID:18779976

  16. Faulty behavior of asynchronous storage elements

    NASA Technical Reports Server (NTRS)

    Al-Assadi, Waleed K.; Lu, Ding; Jayasumana, Anura P.; Malaiya, Yashwant K.; Tong, Carol Q.

    1993-01-01

    It is often assumed that the faults in storage elements (SE's) can be modeled as output/input stuck-at-faults of the element. They are implicitly considered equivalent to the stuck-at faults in the combinational logic surrounding the SE cells. A more accurate higher level fault model for elementary SE's used in asynchronous circuits is presented. This model offers better representation of the physical failures. It is shown that the stuck-at model may be adequate if only modest fault coverage is desired. The enhanced model includes some common fault behaviors of SE's that are not covered by the stuck-at model. These include data-feed-through behaviors that cause the SE to be combinational. Fault models for complex SE cells can be obtained without a significant loss of information about the structure of the circuit.

  17. Asynchronous parallel pattern search for nonlinear optimization

    SciTech Connect

    P. D. Hough; T. G. Kolda; V. J. Torczon

    2000-01-01

    Parallel pattern search (PPS) can be quite useful for engineering optimization problems characterized by a small number of variables (say 10--50) and by expensive objective function evaluations such as complex simulations that take from minutes to hours to run. However, PPS, which was originally designed for execution on homogeneous and tightly-coupled parallel machine, is not well suited to the more heterogeneous, loosely-coupled, and even fault-prone parallel systems available today. Specifically, PPS is hindered by synchronization penalties and cannot recover in the event of a failure. The authors introduce a new asynchronous and fault tolerant parallel pattern search (AAPS) method and demonstrate its effectiveness on both simple test problems as well as some engineering optimization problems

  18. Ultrawideband asynchronous tracking system and method

    NASA Technical Reports Server (NTRS)

    Arndt, G. Dickey (Inventor); Ngo, Phong H. (Inventor); Phan, Chau T. (Inventor); Gross, Julia A. (Inventor); Ni, Jianjun (Inventor); Dusl, John (Inventor)

    2012-01-01

    A passive tracking system is provided with a plurality of ultrawideband (UWB) receivers that is asynchronous with respect to a UWB transmitter. A geometry of the tracking system may utilize a plurality of clusters with each cluster comprising a plurality of antennas. Time Difference of Arrival (TDOA) may be determined for the antennas in each cluster and utilized to determine Angle of Arrival (AOA) based on a far field assumption regarding the geometry. Parallel software communication sockets may be established with each of the plurality of UWB receivers. Transfer of waveform data may be processed by alternately receiving packets of waveform data from each UWB receiver. Cross Correlation Peak Detection (CCPD) is utilized to estimate TDOA information to reduce errors in a noisy, multipath environment.

  19. Structural elements that underlie Doc2β function during asynchronous synaptic transmission

    PubMed Central

    Xue, Renhao; Gaffaney, Jon D.; Chapman, Edwin R.

    2015-01-01

    Double C2-like domain-containing proteins alpha and beta (Doc2α and Doc2β) are tandem C2-domain proteins proposed to function as Ca2+ sensors for asynchronous neurotransmitter release. Here, we systematically analyze each of the negatively charged residues that mediate binding of Ca2+ to the β isoform. The Ca2+ ligands in the C2A domain were dispensable for Ca2+-dependent translocation to the plasma membrane, with one exception: neutralization of D220 resulted in constitutive translocation. In contrast, three of the five Ca2+ ligands in the C2B domain are required for translocation. Importantly, translocation was correlated with the ability of the mutants to enhance asynchronous release when overexpressed in neurons. Finally, replacement of specific Ca2+/lipid-binding loops of synaptotagmin 1, a Ca2+ sensor for synchronous release, with corresponding loops from Doc2β, resulted in chimeras that yielded slower kinetics in vitro and slower excitatory postsynaptic current decays in neurons. Together, these data reveal the key determinants of Doc2β that underlie its function during the slow phase of synaptic transmission. PMID:26195798

  20. Structural elements that underlie Doc2β function during asynchronous synaptic transmission.

    PubMed

    Xue, Renhao; Gaffaney, Jon D; Chapman, Edwin R

    2015-08-01

    Double C2-like domain-containing proteins alpha and beta (Doc2α and Doc2β) are tandem C2-domain proteins proposed to function as Ca(2+) sensors for asynchronous neurotransmitter release. Here, we systematically analyze each of the negatively charged residues that mediate binding of Ca(2+) to the β isoform. The Ca(2+) ligands in the C2A domain were dispensable for Ca(2+)-dependent translocation to the plasma membrane, with one exception: neutralization of D220 resulted in constitutive translocation. In contrast, three of the five Ca(2+) ligands in the C2B domain are required for translocation. Importantly, translocation was correlated with the ability of the mutants to enhance asynchronous release when overexpressed in neurons. Finally, replacement of specific Ca(2+)/lipid-binding loops of synaptotagmin 1, a Ca(2+) sensor for synchronous release, with corresponding loops from Doc2β, resulted in chimeras that yielded slower kinetics in vitro and slower excitatory postsynaptic current decays in neurons. Together, these data reveal the key determinants of Doc2β that underlie its function during the slow phase of synaptic transmission. PMID:26195798

  1. Stand-alone and hybrid positioning using asynchronous pseudolites.

    PubMed

    Gioia, Ciro; Borio, Daniele

    2015-01-01

    global navigation satellite system (GNSS) receivers are usually unable to achieve satisfactory performance in difficult environments, such as open-pit mines, urban canyons and indoors. Pseudolites have the potential to extend GNSS usage and significantly improve receiver performance in such environments by providing additional navigation signals. This also applies to asynchronous pseudolite systems, where different pseudolites operate in an independent way. Asynchronous pseudolite systems require, however, dedicated strategies in order to properly integrate GNSS and pseudolite measurements. In this paper, several asynchronous pseudolite/GNSS integration strategies are considered: loosely- and tightly-coupled approaches are developed and combined with pseudolite proximity and receiver signal strength (RSS)-based positioning. The performance of the approaches proposed has been tested in different scenarios, including static and kinematic conditions. The tests performed demonstrate that the methods developed are effective techniques for integrating heterogeneous measurements from different sources, such as asynchronous pseudolites and GNSS. PMID:25609041

  2. Modeling and Analysis of Mixed Synchronous/Asynchronous Systems

    NASA Technical Reports Server (NTRS)

    Driscoll, Kevin R.; Madl. Gabor; Hall, Brendan

    2012-01-01

    Practical safety-critical distributed systems must integrate safety critical and non-critical data in a common platform. Safety critical systems almost always consist of isochronous components that have synchronous or asynchronous interface with other components. Many of these systems also support a mix of synchronous and asynchronous interfaces. This report presents a study on the modeling and analysis of asynchronous, synchronous, and mixed synchronous/asynchronous systems. We build on the SAE Architecture Analysis and Design Language (AADL) to capture architectures for analysis. We present preliminary work targeted to capture mixed low- and high-criticality data, as well as real-time properties in a common Model of Computation (MoC). An abstract, but representative, test specimen system was created as the system to be modeled.

  3. A Comparison of Distance Education Competencies Delivered Synchronously and Asynchronously.

    ERIC Educational Resources Information Center

    Dooley, Kim E.; Lindner, James R.; Richards, Lance J.

    2003-01-01

    Comparison of agriculture courses delivered synchronously via interactive television (20 students) and asynchronously via the Web (22 students) showed similar achievement of core distance education competencies: adult learning, technology knowledge, instructional design, communication skills, graphic design, administration) regardless of delivery…

  4. Stand-Alone and Hybrid Positioning Using Asynchronous Pseudolites

    PubMed Central

    Gioia, Ciro; Borio, Daniele

    2015-01-01

    global navigation satellite system (GNSS) receivers are usually unable to achieve satisfactory performance in difficult environments, such as open-pit mines, urban canyons and indoors. Pseudolites have the potential to extend GNSS usage and significantly improve receiver performance in such environments by providing additional navigation signals. This also applies to asynchronous pseudolite systems, where different pseudolites operate in an independent way. Asynchronous pseudolite systems require, however, dedicated strategies in order to properly integrate GNSS and pseudolite measurements. In this paper, several asynchronous pseudolite/GNSS integration strategies are considered: loosely- and tightly-coupled approaches are developed and combined with pseudolite proximity and receiver signal strength (RSS)-based positioning. The performance of the approaches proposed has been tested in different scenarios, including static and kinematic conditions. The tests performed demonstrate that the methods developed are effective techniques for integrating heterogeneous measurements from different sources, such as asynchronous pseudolites and GNSS. PMID:25609041

  5. Solving SAT and Hamiltonian Cycle Problem Using Asynchronous P Systems

    NASA Astrophysics Data System (ADS)

    Tagawa, Hirofumi; Fujiwara, Akihiro

    In the present paper, we consider fully asynchronous parallelism in membrane computing, and propose two asynchronous P systems for the satisfiability (SAT) and Hamiltonian cycle problem. We first propose an asynchronous P system that solves SAT with n variables and m clauses, and show that the proposed P system computes SAT in O(mn2n) sequential steps or O(mn) parallel steps using O(mn) kinds of objects. We next propose an asynchronous P system that solves the Hamiltonian cycle problem with n nodes, and show that the proposed P system computes the problem in O(n!) sequential steps or O(n2) parallel steps using O(n2) kinds of objects.

  6. Asynchronous Communication of TLNS3DMB Boundary Exchange

    NASA Technical Reports Server (NTRS)

    Hammond, Dana P.

    1997-01-01

    This paper describes the recognition of implicit serialization due to coarse-grain, synchronous communication and demonstrates the conversion to asynchronous communication for the exchange of boundary condition information in the Thin-Layer Navier Stokes 3-Dimensional Multi Block (TLNS3DMB) code. The implementation details of using asynchronous communication is provided including buffer allocation, message identification, and barrier control. The IBM SP2 was used for the tests presented.

  7. Selective-field-ionization dynamics of a lithium m=2 Rydberg state: Landau-Zener model versus quantal approach

    SciTech Connect

    Foerre, M.; Hansen, J.P.

    2003-05-01

    The selective-field-ionization (SFI) dynamics of a Rydberg state of lithium with magnetic quantum number m=2 is studied in detail based on two different theoretical models: (1) a close coupling integration of the Schroedinger equation and (2) the multichannel (incoherent) Landau-Zener (MLZ) model. The m=2 states are particularly interesting, since they define a border zone between fully adiabatic (m=0,1) and fully diabatic (m>2) ionization dynamics. Both sets of calculations are performed up to, and above, the classical ionization limit. It is found that the MLZ model is excellent in the description of the fully diabatic dynamics while certain discrepancies between the time dependent quantal amplitudes appear when the dynamics become involved. Thus, in this region, the analysis of experimental SFI spectra should be performed with care.

  8. Building asynchronous geospatial processing workflows with web services

    NASA Astrophysics Data System (ADS)

    Zhao, Peisheng; Di, Liping; Yu, Genong

    2012-02-01

    Geoscience research and applications often involve a geospatial processing workflow. This workflow includes a sequence of operations that use a variety of tools to collect, translate, and analyze distributed heterogeneous geospatial data. Asynchronous mechanisms, by which clients initiate a request and then resume their processing without waiting for a response, are very useful for complicated workflows that take a long time to run. Geospatial contents and capabilities are increasingly becoming available online as interoperable Web services. This online availability significantly enhances the ability to use Web service chains to build distributed geospatial processing workflows. This paper focuses on how to orchestrate Web services for implementing asynchronous geospatial processing workflows. The theoretical bases for asynchronous Web services and workflows, including asynchrony patterns and message transmission, are examined to explore different asynchronous approaches to and architecture of workflow code for the support of asynchronous behavior. A sample geospatial processing workflow, issued by the Open Geospatial Consortium (OGC) Web Service, Phase 6 (OWS-6), is provided to illustrate the implementation of asynchronous geospatial processing workflows and the challenges in using Web Services Business Process Execution Language (WS-BPEL) to develop them.

  9. Asynchronous blepharospasm, facial and cervical dystonia, and bilateral asynchronous hemifacial spasm.

    PubMed

    Katz, Bradley J; Burroughs, John R; Anderson, Richard L; Bownds, Shannon; McCann, John D

    2007-01-15

    We present a patient with a facial movement disorder that has characteristics of both blepharospasm and bilateral asynchronous hemifacial spasm. Because of the increased incidence of blepharospasm in patients with hemifacial spasm, our patient's clinical presentation is probably not a chance occurrence, but rather a manifestation of some predisposition for these two movement disorders. This unusual constellation of signs and symptoms challenges the current diagnostic criteria and suggests that some of these facial movement disorders may lie on a spectrum, rather than represent distinct entities. PMID:17133455

  10. Asynchronous origins of ectomycorrhizal clades of Agaricales

    PubMed Central

    Ryberg, Martin; Matheny, P. Brandon

    2012-01-01

    The ectomycorrhizal (ECM) symbiosis is the most widespread biotrophic nutritional mode in mushroom-forming fungi. ECM fungi include, though are not limited to, about 5000 described species of Agaricales from numerous, independently evolved lineages. Two central hypotheses suggest different explanations for the origin of ECM fungal diversity: (i) dual origins, initially with the Pinaceae in the Jurassic and later with angiosperms during the Late Cretaceous, and (ii) a simultaneous and convergent radiation of ECM lineages in response to cooling climate during the Palaeogene and advancing temperate ECM plant communities. Neither of these hypotheses is supported here. While we demonstrate support for asynchronous origins of ECM Agaricales, the timing of such events appears to have occurred more recently than suggested by the first hypothesis, first during the Cretaceous and later during the Palaeogene. We are also unable to reject models of rate constancy, which suggests that the diversity of ECM Agaricales is not a consequence of convergent rapid radiations following evolutionary transitions from saprotrophic to ECM habits. ECM lineages of Agaricales differ not only in age, but also in rates of diversification and rate of substitution at nuclear ribosomal RNA loci. These results question the biological uniformity of the ECM guild. PMID:22171078

  11. Rapid, generalized adaptation to asynchronous audiovisual speech

    PubMed Central

    Van der Burg, Erik; Goodbourn, Patrick T.

    2015-01-01

    The brain is adaptive. The speed of propagation through air, and of low-level sensory processing, differs markedly between auditory and visual stimuli; yet the brain can adapt to compensate for the resulting cross-modal delays. Studies investigating temporal recalibration to audiovisual speech have used prolonged adaptation procedures, suggesting that adaptation is sluggish. Here, we show that adaptation to asynchronous audiovisual speech occurs rapidly. Participants viewed a brief clip of an actor pronouncing a single syllable. The voice was either advanced or delayed relative to the corresponding lip movements, and participants were asked to make a synchrony judgement. Although we did not use an explicit adaptation procedure, we demonstrate rapid recalibration based on a single audiovisual event. We find that the point of subjective simultaneity on each trial is highly contingent upon the modality order of the preceding trial. We find compelling evidence that rapid recalibration generalizes across different stimuli, and different actors. Finally, we demonstrate that rapid recalibration occurs even when auditory and visual events clearly belong to different actors. These results suggest that rapid temporal recalibration to audiovisual speech is primarily mediated by basic temporal factors, rather than higher-order factors such as perceived simultaneity and source identity. PMID:25716790

  12. Asynchronous electrical activity in epileptic seizures

    NASA Astrophysics Data System (ADS)

    Holman, Katherine; Lim, Eugene; Gliske, Stephen; Stacey, William; Fink, Christian

    High-frequency oscillations (HFOs) have been postulated to be potential biomarkers for focal epileptic seizures, with fast ripples (>250 Hz) as the most interesting candidate. The mechanisms underlying the generation of fast ripples, however, are not well understood. In this study, we draw upon results from previous computational studies on HFOs to develop a new mathematical model from first principles describing the generation of HFOs through asynchronous neuronal firing. Asynchrony in the model is obtained with the introduction of two parameters of heterogeneity: variability in the inter-spike interval (ISI) and jitter. The model predicts the generation of harmonic narrow-band oscillations if the heterogeneity-governing parameters do not differ from the predefined ISI by more than 20%. Comparisons against results from a separately constructed computational model verify the accuracy of the model in study. These results provide us with a rigorous framework in which we may investigate the mechanisms driving the generation of abnormal HFOs, and may serve as groundwork for future research in epileptogenesis. Nsf Grant 1003992, Ohio Wesleyan University SSRP.

  13. Asynchronous Rate Chaos in Spiking Neuronal Circuits.

    PubMed

    Harish, Omri; Hansel, David

    2015-07-01

    The brain exhibits temporally complex patterns of activity with features similar to those of chaotic systems. Theoretical studies over the last twenty years have described various computational advantages for such regimes in neuronal systems. Nevertheless, it still remains unclear whether chaos requires specific cellular properties or network architectures, or whether it is a generic property of neuronal circuits. We investigate the dynamics of networks of excitatory-inhibitory (EI) spiking neurons with random sparse connectivity operating in the regime of balance of excitation and inhibition. Combining Dynamical Mean-Field Theory with numerical simulations, we show that chaotic, asynchronous firing rate fluctuations emerge generically for sufficiently strong synapses. Two different mechanisms can lead to these chaotic fluctuations. One mechanism relies on slow I-I inhibition which gives rise to slow subthreshold voltage and rate fluctuations. The decorrelation time of these fluctuations is proportional to the time constant of the inhibition. The second mechanism relies on the recurrent E-I-E feedback loop. It requires slow excitation but the inhibition can be fast. In the corresponding dynamical regime all neurons exhibit rate fluctuations on the time scale of the excitation. Another feature of this regime is that the population-averaged firing rate is substantially smaller in the excitatory population than in the inhibitory population. This is not necessarily the case in the I-I mechanism. Finally, we discuss the neurophysiological and computational significance of our results. PMID:26230679

  14. Asynchronous event-based hebbian epipolar geometry.

    PubMed

    Benosman, Ryad; Ieng, Sio-Hoï; Rogister, Paul; Posch, Christoph

    2011-11-01

    Epipolar geometry, the cornerstone of perspective stereo vision, has been studied extensively since the advent of computer vision. Establishing such a geometric constraint is of primary importance, as it allows the recovery of the 3-D structure of scenes. Estimating the epipolar constraints of nonperspective stereo is difficult, they can no longer be defined because of the complexity of the sensor geometry. This paper will show that these limitations are, to some extent, a consequence of the static image frames commonly used in vision. The conventional frame-based approach suffers from a lack of the dynamics present in natural scenes. We introduce the use of neuromorphic event-based--rather than frame-based--vision sensors for perspective stereo vision. This type of sensor uses the dimension of time as the main conveyor of information. In this paper, we present a model for asynchronous event-based vision, which is then used to derive a general new concept of epipolar geometry linked to the temporal activation of pixels. Practical experiments demonstrate the validity of the approach, solving the problem of estimating the fundamental matrix applied, in a first stage, to classic perspective vision and then to more general cameras. Furthermore, this paper shows that the properties of event-based vision sensors allow the exploration of not-yet-defined geometric relationships, finally, we provide a definition of general epipolar geometry deployable to almost any visual sensor. PMID:21954205

  15. Rapid, generalized adaptation to asynchronous audiovisual speech.

    PubMed

    Van der Burg, Erik; Goodbourn, Patrick T

    2015-04-01

    The brain is adaptive. The speed of propagation through air, and of low-level sensory processing, differs markedly between auditory and visual stimuli; yet the brain can adapt to compensate for the resulting cross-modal delays. Studies investigating temporal recalibration to audiovisual speech have used prolonged adaptation procedures, suggesting that adaptation is sluggish. Here, we show that adaptation to asynchronous audiovisual speech occurs rapidly. Participants viewed a brief clip of an actor pronouncing a single syllable. The voice was either advanced or delayed relative to the corresponding lip movements, and participants were asked to make a synchrony judgement. Although we did not use an explicit adaptation procedure, we demonstrate rapid recalibration based on a single audiovisual event. We find that the point of subjective simultaneity on each trial is highly contingent upon the modality order of the preceding trial. We find compelling evidence that rapid recalibration generalizes across different stimuli, and different actors. Finally, we demonstrate that rapid recalibration occurs even when auditory and visual events clearly belong to different actors. These results suggest that rapid temporal recalibration to audiovisual speech is primarily mediated by basic temporal factors, rather than higher-order factors such as perceived simultaneity and source identity. PMID:25716790

  16. Gigabit Ethernet Asynchronous Clock Compensation FIFO

    NASA Technical Reports Server (NTRS)

    Duhachek, Jeff

    2012-01-01

    Clock compensation for Gigabit Ethernet is necessary because the clock recovered from the 1.25 Gb/s serial data stream has the potential to be 200 ppm slower or faster than the system clock. The serial data is converted to 10-bit parallel data at a 125 MHz rate on a clock recovered from the serial data stream. This recovered data needs to be processed by a system clock that is also running at a nominal rate of 125 MHz, but not synchronous to the recovered clock. To cross clock domains, an asynchronous FIFO (first-in-first-out) is used, with the write pointer (wprt) in the recovered clock domain and the read pointer (rptr) in the system clock domain. Because the clocks are generated from separate sources, there is potential for FIFO overflow or underflow. Clock compensation in Gigabit Ethernet is possible by taking advantage of the protocol data stream features. There are two distinct data streams that occur in Gigabit Ethernet where identical data is transmitted for a period of time. The first is configuration, which happens during auto-negotiation. The second is idle, which occurs at the end of auto-negotiation and between every packet. The identical data in the FIFO can be repeated by decrementing the read pointer, thus compensating for a FIFO that is draining too fast. The identical data in the FIFO can also be skipped by incrementing the read pointer, which compensates for a FIFO draining too slowly. The unique and novel features of this FIFO are that it works in both the idle stream and the configuration streams. The increment or decrement of the read pointer is different in the idle and compensation streams to preserve disparity. Another unique feature is that the read pointer to write pointer difference range changes between compensation and idle to minimize FIFO latency during packet transmission.

  17. Asynchronous reference frame agreement in a quantum network

    NASA Astrophysics Data System (ADS)

    Islam, Tanvirul; Wehner, Stephanie

    2016-03-01

    An efficient implementation of many multiparty protocols for quantum networks requires that all the nodes in the network share a common reference frame. Establishing such a reference frame from scratch is especially challenging in an asynchronous network where network links might have arbitrary delays and the nodes do not share synchronised clocks. In this work, we study the problem of establishing a common reference frame in an asynchronous network of n nodes of which at most t are affected by arbitrary unknown error, and the identities of the faulty nodes are not known. We present a protocol that allows all the correctly functioning nodes to agree on a common reference frame as long as the network graph is complete and not more than t\\lt n/4 nodes are faulty. As the protocol is asynchronous, it can be used with some assumptions to synchronise clocks over a network. Also, the protocol has the appealing property that it allows any existing two-node asynchronous protocol for reference frame agreement to be lifted to a robust protocol for an asynchronous quantum network.

  18. Extended Self Organised Criticality in Asynchronously Tuned Cellular Automata

    NASA Astrophysics Data System (ADS)

    Gunji, Yukio-Pegio

    2014-12-01

    Systems at a critical point in phase transitions can be regarded as being relevant to biological complex behaviour. Such a perspective can only result, in a mathematical consistent manner, from a recursive structure. We implement a recursive structure based on updating by asynchronously tuned elementary cellular automata (AT ECA), and show that a large class of elementary cellular automata (ECA) can reveal critical behavior due to the asynchronous updating and tuning.We show that the obtained criticality coincides with the criticality in phase transitions of asynchronous ECA with respect to density decay, and that multiple distributed ECAs, synchronously updated, can emulate critical behavior in AT ECA. Our approach draws on concepts and tools from category and set theory, in particular on "adjunction dualities" of pairs of adjoint functors.

  19. Simulating fail-stop in asynchronous distributed systems

    NASA Technical Reports Server (NTRS)

    Sabel, Laura; Marzullo, Keith

    1994-01-01

    The fail-stop failure model appears frequently in the distributed systems literature. However, in an asynchronous distributed system, the fail-stop model cannot be implemented. In particular, it is impossible to reliably detect crash failures in an asynchronous system. In this paper, we show that it is possible to specify and implement a failure model that is indistinguishable from the fail-stop model from the point of view of any process within an asynchronous system. We give necessary conditions for a failure model to be indistinguishable from the fail-stop model, and derive lower bounds on the amount of process replication needed to implement such a failure model. We present a simple one-round protocol for implementing one such failure model, which we call simulated fail-stop.

  20. Asynchronous event-based corner detection and matching.

    PubMed

    Clady, Xavier; Ieng, Sio-Hoi; Benosman, Ryad

    2015-06-01

    This paper introduces an event-based luminance-free method to detect and match corner events from the output of asynchronous event-based neuromorphic retinas. The method relies on the use of space-time properties of moving edges. Asynchronous event-based neuromorphic retinas are composed of autonomous pixels, each of them asynchronously generating "spiking" events that encode relative changes in pixels' illumination at high temporal resolutions. Corner events are defined as the spatiotemporal locations where the aperture problem can be solved using the intersection of several geometric constraints in events' spatiotemporal spaces. A regularization process provides the required constraints, i.e. the motion attributes of the edges with respect to their spatiotemporal locations using local geometric properties of visual events. Experimental results are presented on several real scenes showing the stability and robustness of the detection and matching. PMID:25828960

  1. Bayesian analysis of the kinetics of quantal transmitter secretion at the neuromuscular junction.

    PubMed

    Saveliev, Anatoly; Khuzakhmetova, Venera; Samigullin, Dmitry; Skorinkin, Andrey; Kovyazina, Irina; Nikolsky, Eugeny; Bukharaeva, Ellya

    2015-10-01

    The timing of transmitter release from nerve endings is considered nowadays as one of the factors determining the plasticity and efficacy of synaptic transmission. In the neuromuscular junction, the moments of release of individual acetylcholine quanta are related to the synaptic delays of uniquantal endplate currents recorded under conditions of lowered extracellular calcium. Using Bayesian modelling, we performed a statistical analysis of synaptic delays in mouse neuromuscular junction with different patterns of rhythmic nerve stimulation and when the entry of calcium ions into the nerve terminal was modified. We have obtained a statistical model of the release timing which is represented as the summation of two independent statistical distributions. The first of these is the exponentially modified Gaussian distribution. The mixture of normal and exponential components in this distribution can be interpreted as a two-stage mechanism of early and late periods of phasic synchronous secretion. The parameters of this distribution depend on both the stimulation frequency of the motor nerve and the calcium ions' entry conditions. The second distribution was modelled as quasi-uniform, with parameters independent of nerve stimulation frequency and calcium entry. Two different probability density functions for the distribution of synaptic delays suggest at least two independent processes controlling the time course of secretion, one of them potentially involving two stages. The relative contribution of these processes to the total number of mediator quanta released depends differently on the motor nerve stimulation pattern and on calcium ion entry into nerve endings. PMID:26129670

  2. Design issues in the semantics and scheduling of asynchronous tasks.

    SciTech Connect

    Olivier, Stephen L.

    2013-07-01

    The asynchronous task model serves as a useful vehicle for shared memory parallel programming, particularly on multicore and manycore processors. As adoption of model among programmers has increased, support has emerged for the integration of task parallel language constructs into mainstream programming languages, e.g., C and C++. This paper examines some of the design decisions in Cilk and OpenMP concerning semantics and scheduling of asynchronous tasks with the aim of informing the efforts of committees considering language integration, as well as developers of new task parallel languages and libraries.

  3. Evaluation of attractors and basins of asynchronous random Boolean networks

    NASA Astrophysics Data System (ADS)

    Yang, Meng; Chu, Tianguang

    2012-05-01

    We present an algebraic approach for determining the attractors and their basins of random Boolean networks under an asynchronous stochastic update based on the recently developed matrix semitensor product theory, which allows for converting the logical dynamics of a Boolean network into a standard iterative dynamics. In this setting, all attractors and basins are specified by the network transition matrices. We then devise procedures that can find all attractors and their basins exactly. We also discuss the issue of overlapping basins in asynchronous random Boolean networks, and we propose methods to compute the weight of each attractor and the basin entropy of the systems.

  4. Asynchronous parallel generating set search for linearly-constrained optimization.

    SciTech Connect

    Lewis, Robert Michael; Griffin, Joshua D.; Kolda, Tamara Gibson

    2006-08-01

    Generating set search (GSS) is a family of direct search methods that encompasses generalized pattern search and related methods. We describe an algorithm for asynchronous linearly-constrained GSS, which has some complexities that make it different from both the asynchronous bound-constrained case as well as the synchronous linearly-constrained case. The algorithm has been implemented in the APPSPACK software framework and we present results from an extensive numerical study using CUTEr test problems. We discuss the results, both positive and negative, and conclude that GSS is a reliable method for solving small-to-medium sized linearly-constrained optimization problems without derivatives.

  5. Significance of microbial asynchronous anabolism to soil carbon dynamics driven by litter inputs

    NASA Astrophysics Data System (ADS)

    Fan, Zhaosheng; Liang, Chao

    2015-04-01

    Soil organic carbon (SOC) plays an important role in the global carbon cycle. However, it remains largely unknown how plant litter inputs impact magnitude, composition and source configuration of the SOC stocks over long term through microbial catabolism and anabolism, mostly due to uncoupled research on litter decomposition and SOC formation. This limits our ability to predict soil system responses to changes in land-use and climate. Here, we examine how microbes act as a valve controlling carbon sequestrated from plant litters versus released to the atmosphere in natural ecosystems amended with plant litters varying in quantity and quality. We find that litter quality - not quantity - regulates long-term SOC dynamics under different plausible scenarios. Long-term changes in bulk SOC stock occur only when the quality of carbon inputs causes asynchronous change in a microbial physiological trait, defined as ``microbial biosynthesis acceleration'' (MBA). This is the first theoretical demonstration that the response of the SOC stocks to litter inputs is critically determined by the microbial physiology. Our work suggests that total SOC at an equilibrium state may be an intrinsic property of a given ecosystem, which ultimately is controlled by the asynchronous MBA between microbial functional groups.

  6. Significance of microbial asynchronous anabolism to soil carbon dynamics driven by litter inputs

    DOE PAGESBeta

    Fan, Zhaosheng; Liang, Chao

    2015-04-02

    Soil organic carbon (SOC) plays an important role in the global carbon cycle. However, it remains largely unknown how plant litter inputs impact magnitude, composition and source configuration of the SOC stocks over long term through microbial catabolism and anabolism, mostly due to uncoupled research on litter decomposition and SOC formation. This limits our ability to predict soil system responses to changes in land-use and climate. Here, we examine how microbes act as a valve controlling carbon sequestrated from plant litters versus released to the atmosphere in natural ecosystems amended with plant litters varying in quantity and quality. We findmore » that litter quality – not quantity – regulates long-term SOC dynamics under different plausible scenarios. Long-term changes in bulk SOC stock occur only when the quality of carbon inputs causes asynchronous change in a microbial physiological trait, defined as ‘‘microbial biosynthesis acceleration’’ (MBA). This is the first theoretical demonstration that the response of the SOC stocks to litter inputs is critically determined by the microbial physiology. Our work suggests that total SOC at an equilibrium state may be an intrinsic property of a given ecosystem, which ultimately is controlled by the asynchronous MBA between microbial functional groups.« less

  7. Significance of microbial asynchronous anabolism to soil carbon dynamics driven by litter inputs

    SciTech Connect

    Fan, Zhaosheng; Liang, Chao

    2015-04-02

    Soil organic carbon (SOC) plays an important role in the global carbon cycle. However, it remains largely unknown how plant litter inputs impact magnitude, composition and source configuration of the SOC stocks over long term through microbial catabolism and anabolism, mostly due to uncoupled research on litter decomposition and SOC formation. This limits our ability to predict soil system responses to changes in land-use and climate. Here, we examine how microbes act as a valve controlling carbon sequestrated from plant litters versus released to the atmosphere in natural ecosystems amended with plant litters varying in quantity and quality. We find that litter quality – not quantity – regulates long-term SOC dynamics under different plausible scenarios. Long-term changes in bulk SOC stock occur only when the quality of carbon inputs causes asynchronous change in a microbial physiological trait, defined as ‘‘microbial biosynthesis acceleration’’ (MBA). This is the first theoretical demonstration that the response of the SOC stocks to litter inputs is critically determined by the microbial physiology. Our work suggests that total SOC at an equilibrium state may be an intrinsic property of a given ecosystem, which ultimately is controlled by the asynchronous MBA between microbial functional groups.

  8. The Influence of Asynchronous Video Communication on Learner Social Presence: A Narrative Analysis of Four Cases

    ERIC Educational Resources Information Center

    Borup, Jered; West, Richard E.; Graham, Charles R.

    2013-01-01

    Online courses are increasingly using asynchronous video communication. However, little is known about how asynchronous video communication influences students' communication patterns. This study presents four narratives of students with varying characteristics who engaged in asynchronous video communication. The extrovert valued the efficiency of…

  9. Quantal Study of the Exchange Reaction for N + N2 using an ab initio Potential Energy Surface

    NASA Technical Reports Server (NTRS)

    Wang, Dunyou; Stallcop, James R.; Huo, Winifred M.; Dateo, Christopher E.; Schwenke, David W.; Partridge, Harry; Kwak, Dochan (Technical Monitor)

    2002-01-01

    The N + N2 exchange rate is calculated using a time-dependent quantum dynamics method on a newly determined ab initio potential energy surface (PES) for the ground A" state. This ab initio PES shows a double barrier feature in the interaction region with the barrier height at 47.2 kcal/mol, and a shallow well between these two barriers, with the minimum at 43.7 kcal/mol. A quantum dynamics wave packet calculation has been carried out using the fitted PES to compute the cumulative reaction probability for the exchange reaction of N + N2(J=O). The J - K shift method is then employed to obtain the rate constant for this reaction. The calculated rate constant is compared with experimental data and a recent quasi-classical calculation using a LEPS PES. Significant differences are found between the present and quasiclassical results. The present rate calculation is the first accurate 3D quantal dynamics study for N + N2 reaction system and the ab initio PES reported here is the first such surface for N3.

  10. Developing a Successful Asynchronous Online Extension Program for Forest Landowners

    ERIC Educational Resources Information Center

    Zobrist, Kevin W.

    2014-01-01

    Asynchronous online Extension classes can reach a wide audience, is convenient for the learner, and minimizes ongoing demands on instructor time. However, producing such classes takes significant effort up front. Advance planning and good communication with contributors are essential to success. Considerations include delivery platforms, content…

  11. Improving the Asynchronous Online Learning Environment Using Discussion Boards

    ERIC Educational Resources Information Center

    Ringler, Ilene; Schubert, Carol; Deem, Jack; Flores, Jimmie; Friestad-Tate, Jill; Lockwood, Robert

    2015-01-01

    Discussion Boards are at the heart of an Asynchronous Online Learning or Distance Education Environment and can have a great impact on the learning experience. Understanding the individual factors that create a high quality discussion board experience for students and their interrelationships is critical to continuous improvement in distance…

  12. Psychological Type and Asynchronous Written Dialogue in Adult Learning

    ERIC Educational Resources Information Center

    Lin, Lin; Cranton, Patricia; Bridglall, Beatrice

    2005-01-01

    This study explores how adults learn from asynchronous written dialogue through the lens of psychological type preferences. We asked participants to discover their dominant and auxiliary psychological preferences using the Personal Empowerment through Type inventory. Participants then completed an open-ended survey in which they described their…

  13. Interaction in Asynchronous Discussion Forums: Peer Facilitation Techniques

    ERIC Educational Resources Information Center

    Ng, C. S. L.; Cheung, W. S.; Hew, K. F.

    2012-01-01

    Peer facilitation is proposed as a solution to counter limited interaction in asynchronous online discussions. However, there is a lack of empirical research on online peer facilitation. This study identifies, through cross-case comparison of two graduate-level blended courses attended by Asian Pacific students, the actual peer facilitation…

  14. Group Learning Environment Linking Synchronous and Asynchronous Learning.

    ERIC Educational Resources Information Center

    Hayashi, Toshihiro; Nakanishi, Yoshinari; Hayashida, Yukuo

    This paper describes the design and features of LEA (Learning Environment with Agent), an educational system which has the features of both synchronous and asynchronous group learning environments. The first section discusses group learning environments, including differences between individual learning and group learning, and the classification…

  15. Comparison of the Asynchronous Differential Evolution and JADE Minimization Algorithms

    NASA Astrophysics Data System (ADS)

    Zhabitsky, Mikhail

    2016-02-01

    Differential Evolution (DE) is an efficient evolutionary algorithm to solve global optimization problems. In this work we compare performance of the recently proposed Asynchronous Differential Evolution with Adaptive Correlation Matrix (ADEACM) to the widely used JADE algorithm, a DE variant with adaptive control parameters.

  16. Analysis of the Relevance of Posts in Asynchronous Discussions

    ERIC Educational Resources Information Center

    Azevedo, Breno T.; Reategui, Eliseo; Behar, Patrícia A.

    2014-01-01

    This paper presents ForumMiner, a tool for the automatic analysis of students' posts in asynchronous discussions. ForumMiner uses a text mining system to extract graphs from texts that are given to students as a basis for their discussion. These graphs contain the most relevant terms found in the texts, as well as the relationships between them.…

  17. Language Use in Asynchronous Computer-Mediated Communication in Taiwan

    ERIC Educational Resources Information Center

    Huang, Daphne Li-jung

    2009-01-01

    This paper describes how Chinese-English bilinguals in Taiwan use their languages in asynchronous computer-mediated communication, specifically, via Bulletin Board System (BBS) and email. The main data includes two types: emails collected from a social network and postings collected from two BBS websites. By examining patterns of language choice…

  18. Engaging Graduate Students in Rich Asynchronous Online Discussions

    ERIC Educational Resources Information Center

    Wuttikietpaiboon, Kanya

    2012-01-01

    Given the importance of online instructional procedures that engage students in rich online discussions and enhance the quantity and the quality of the discussions, the purpose of this study was to investigate how a particular online instructor engaged graduate students in rich asynchronous online discussions, what roles the instructor played to…

  19. Fostering Knowledge Construction in University Students through Asynchronous Discussion Groups

    ERIC Educational Resources Information Center

    Schellens, Tammy; Valcke, Martin

    2006-01-01

    Does collaborative learning in asynchronous discussion groups result in enhancing academic discourse and knowledge construction? This general research question has been researched in a study involving 300 students, working during six months in 38 electronic discussion groups. The transcripts of the discussions were coded and analysed to test…

  20. Asynchronous Education: A Blueprint for the Future of Adult Learning.

    ERIC Educational Resources Information Center

    Krueger, Lyle L.; Porter, Cyndi Wilson; Burke, Daniel

    While the distance learning curriculum at Lakeland College (Wisconsin) began 20 years ago, the idea for Lakeland Online was conceived about two and one-half years ago. A variety of delivery modes were considered before discovering Convene software. Since the communication process enabled by the Convene software is asynchronous and requires only a…

  1. Content Analysis Coding Schemes for Online Asynchronous Discussion

    ERIC Educational Resources Information Center

    Weltzer-Ward, Lisa

    2011-01-01

    Purpose: Researchers commonly utilize coding-based analysis of classroom asynchronous discussion contributions as part of studies of online learning and instruction. However, this analysis is inconsistent from study to study with over 50 coding schemes and procedures applied in the last eight years. The aim of this article is to provide a basis…

  2. Cyber Asynchronous versus Blended Cyber Approach in Distance English Learning

    ERIC Educational Resources Information Center

    Ge, Zi-Gang

    2012-01-01

    This study aims to compare the single cyber asynchronous learning approach with the blended cyber learning approach in distance English education. Two classes of 70 students participated in this study, which lasted one semester of about four months, with one class using the blended approach for their English study and the other only using the…

  3. Reflections of Students in Their Use of Asynchronous Online Seminars

    ERIC Educational Resources Information Center

    Groves, Mark; O'Donoghue, John

    2009-01-01

    This paper reports on research that has been carried out into the use, process, and effectiveness of an asynchronous online seminar within an undergraduate sports studies degree programme. Contemporary sources are used to justify the use of technology supported learning (TSL) in higher education and to inform a reflective and critical account of…

  4. Students' Activity Focus in Online Asynchronous Peer Learning Forums

    ERIC Educational Resources Information Center

    Gaspar, Alessio; Langevin, Sarah; Boyer, Naomi; Armitage, William

    2010-01-01

    This qualitative study explores how using Peer Learning Forums (PLF) in an online asynchronous computer programming course can be analyzed to derive information about Student Activity Focus (SAF) for adult Information Technology students. Three instruments are proposed to assist instructors classify questions posted by students on these forums,…

  5. Student Moderators in Asynchronous Online Discussion: A Question of Questions

    ERIC Educational Resources Information Center

    Zingaro, Daniel

    2012-01-01

    Much current research exalts the benefits of having students facilitate weekly discussions in asynchronous online courses. This study seeks to add to what is known about student moderation through an analysis of the types of questions students use to spur each discussion. Prior experimental work has demonstrated that the types of questions posed…

  6. An Investigation of Student Practices in Asynchronous Computer Conferencing Courses

    ERIC Educational Resources Information Center

    Peters, Vanessa L.; Hewitt, Jim

    2010-01-01

    This study investigated the online practices of students enrolled in graduate-level distance education courses. Using interviews and a questionnaire as data sources, the study sought to: (a) identify common practices that students adopt in asynchronous discussions, and (b) gain an understanding of why students adopt them. An analysis of the data…

  7. Promoting and Scaffolding Argumentation through Reflective Asynchronous Discussions

    ERIC Educational Resources Information Center

    Lin, Huann-shyang; Hong, Zuway-R.; Lawrenz, Frances

    2012-01-01

    The purpose of this quasi-experimental study was to explore the impact of asynchronous discussion on the quality and complexity of college students' arguments. Three different cohorts of students registered in a physical science course in 2009 Fall, 2010 Spring, and 2010 Fall semesters were briefly supported with scaffolding in class and then…

  8. Reconceptualising Moderation in Asynchronous Online Discussions Using Grounded Theory

    ERIC Educational Resources Information Center

    Vlachopoulos, Panos; Cowan, John

    2010-01-01

    This article reports a grounded theory study of the moderation of asynchronous online discussions, to explore the processes by which tutors in higher education decide when and how to moderate. It aims to construct a theory of e-moderation based on some key factors which appear to influence e-moderation. It discusses previous research on the…

  9. Superconducting magnetic energy storage for asynchronous electrical systems

    DOEpatents

    Boenig, H.J.

    1984-05-16

    It is an object of the present invention to provide superconducting magnetic energy storage for a plurality of asynchronous electrical systems. It is a further object of the present invention to provide load leveling and stability improvement in a plurality of independent ac systems using a single superconducting magnetic energy storage coil.

  10. Online Graduate Education: Developing Scholars through Asynchronous Discussion

    ERIC Educational Resources Information Center

    Bowden, Randall

    2012-01-01

    Considerable effort has been placed on understanding and enhancing online interaction to increase student learning, examine teaching strategies, and build learning communities. This research explored another aspect of interaction: the emergence of scholarship by graduate students through asynchronous discussion. Qualitative analysis of archived…

  11. Asynchronous Torque Characteristics of VR Type Vernier Motor

    NASA Astrophysics Data System (ADS)

    Suda, Hiroshi; Matsushima, Yoshitaro; Xu, Li; Anazawa, Yoshihisa

    Recently, various types of vernier motor are developed. The VR type vernier motor is a kind of three-phase reluctance motor, and its stator has slots and distributed three-phase windings. The rotor is slotted iron core without windings. Relationship between S, R and P is S = R±2, where S and R are the numbers of stator and rotor slots per pair of poles, and the number of poles P is two. The rotor of the vernier motor moves at a sub-multiple of the angular velocity of the stator mmf. The multiplying factor is P/R. Authors had reported that the VR type vernier motors are analyzed as a three-phase reluctance motor, and have obtained the voltage equations on the γ-δ axis and expression of the torque.However, the asynchronous characteristics of the vernier motor are not clear yet. In this paper, the asynchronous characteristics of the vernier motors are analyzed with the symmetric components transformation and the commutator transformation. The voltage equations on the symmetrical co-ordinates and asynchronous and synchronous torque expression at steady state condition are obtained. The tests were performed on the trial motor. The calculated asynchronous torque has agreed well with the measured values.

  12. Asynchronous Assessment in a Large Lecture Marketing Course

    ERIC Educational Resources Information Center

    Downey, W. Scott; Schetzsle, Stacey

    2012-01-01

    Asynchronous assessment, which includes quizzes or exams online or outside class, offers marketing educators an opportunity to make more efficient use of class time and to enhance students' learning experiences by giving them more flexibility and choice in their assessment environment. In this paper, we examine the performance difference between…

  13. The Socratic Dialogue in Asynchronous Online Discussions: Is Constructivism Redundant?

    ERIC Educational Resources Information Center

    Kingsley, Paul

    2011-01-01

    Purpose: This paper aims to examine Socratic dialogue in asynchronous online discussions in relation to constructivism. The links between theory and practice in teaching are to be discussed whilst tracing the origins of Socratic dialogue and recent trends and use of seminar in research based institutions. Design/methodology/approach: Many online…

  14. Maintained cocaine self-administration is determined by quantal responses: implications for the measurement of antagonist potency.

    PubMed

    Norman, Andrew B; Tabet, Michael R; Norman, Mantana K; Tsibulsky, Vladimir L

    2014-02-01

    The change in frequency of cocaine self-administration as a function of the unit dose is widely assumed to represent a graded pharmacodynamic response. Alternatively, a pharmacological theory states that during maintained self-administration, a quantal response occurs at a minimum maintained cocaine concentration (satiety threshold). Rats self-administered cocaine at unit doses spanning an 8-fold range from 0.75 to 6 µmol/kg. Despite an approximately 7-fold difference in the interinjection intervals, there were no differences in the plasma cocaine concentration at the time of lever press across this range of unit doses, consistent with the satiety threshold representing an equiactive cocaine concentration. Because self-administration always occurs when cocaine concentrations decline back to the satiety threshold, this behavior represents a process of automatic back titration of equiactive agonist concentrations. Therefore, the lower frequency of self-administration at higher unit doses is caused by an increase in the duration of the cocaine-induced satiety response, and the graded dose-frequency relationship is due to cocaine pharmacokinetics. After the interinjection intervals at a particular unit dose were stable, rats were injected with the competitive D₁-like dopamine receptor antagonist R-(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine (SCH23390; 15 nmol/kg intravenously) and the session continued. At all cocaine unit doses, SCH23390 accelerated self-administration with a concomitant increase in the calculated satiety threshold, and these equiactive cocaine concentration ratios were independent of the cocaine unit dose. Therefore, the measurement of antagonist potency requires only a single unit dose of cocaine, selected on the basis of convenience, and using multiple cocaine unit doses is redundant. PMID:24307200

  15. Striatal dopamine neurotransmission: regulation of release and uptake

    PubMed Central

    Sulzer, David; Cragg, Stephanie J.; Rice, Margaret E.

    2016-01-01

    Dopamine (DA) transmission is governed by processes that regulate release from axonal boutons in the forebrain and the somatodendritic compartment in midbrain, and by clearance by the DA transporter, diffusion, and extracellular metabolism. We review how axonal DA release is regulated by neuronal activity and by autoreceptors and heteroreceptors, and address how quantal release events are regulated in size and frequency. In brain regions densely innervated by DA axons, DA clearance is due predominantly to uptake by the DA transporter, whereas in cortex, midbrain, and other regions with relatively sparse DA inputs, the norepinephrine transporter and diffusion are involved. We discuss the role of DA uptake in restricting the sphere of influence of DA and in temporal accumulation of extracellular DA levels upon successive action potentials. The tonic discharge activity of DA neurons may be translated into a tonic extracellular DA level, whereas their bursting activity can generate discrete extracellular DA transients. PMID:27141430

  16. Asynchronous telehealth: a scoping review of analytic studies

    PubMed Central

    Deshpande, Amol; Khoja, Shariq; Lorca, Julio; McKibbon, Ann; Rizo, Carlos; Husereau, Donald; Jadad, Alejandro R

    2009-01-01

    Background Asynchronous telehealth captures clinically important digital samples (e.g., still images, video, audio, text files) and relevant data in one location and subsequently transmits these files for interpretation at a remote site by health professionals without requiring the simultaneous presence of the patient involved and his or her health care provider. Its utility in the health care system, however, still remains poorly defined. We conducted this scoping review to determine the impact of asynchronous telehealth on health outcomes, process of care, access to health services, and health resources. Methods A search was performed up to December 2006 of MEDLINE, CINAHL, HealthSTAR, the Database of Abstracts of Reviews of Effectiveness, and The Cochrane Library. Studies were included if they contained original data on the use of asynchronous telehealth and were published in English in a peer-reviewed journal. Two independent reviewers screened all articles and extracted data, reaching consensus on the articles and data identified. Data were extracted on general study characteristics, clinical domain, technology, setting, category of outcome, and results. Study quality (internal validity) was assessed using the Jadad scale for randomized controlled trials and the Downs and Black index for non-randomized studies. Summary data were categorized by medical specialty and presented qualitatively. Results The scoping review included 52 original studies from 238 citations identified; of these 52, almost half focused on the use of telehealth in dermatology. Included studies were characterized by diverse designs, interventions, and outcomes. Only 16 studies were judged to be of high quality. Most studies showed beneficial effects in terms of diagnostic accuracy, wait times, referral management, and satisfaction with services. Evidence on the impact of asynchronous telehealth on resource use in dermatology suggests a reduction in the number of, or avoidance of, in

  17. LETTER TO THE EDITOR: The validity of classical trajectory and perturbative quantal methods for electron-impact ionization from excited states in H-like ions

    NASA Astrophysics Data System (ADS)

    Griffin, D. C.; Ballance, C. P.; Pindzola, M. S.; Robicheaux, F.; Loch, S. D.; Ludlow, J. A.; Witthoeft, M. C.; Colgan, J.; Fontes, C. J.; Schultz, D. R.

    2005-06-01

    To test the validity of classical trajectory and perturbative quantal methods for electron-impact ionization of H-like ions from excited states, we have performed advanced close-coupling calculations of ionization from excited states in H, Li2+ and B4+ using the R-matrix with pseudo states and the time-dependent close-coupling methods. Comparisons with our classical trajectory Monte Carlo (CTMC) and distorted-wave (DW) calculations show that the CTMC method is more accurate than the DW method for H, but does not improve with n and grows substantially worse with Z, while the DW method improves with Z and grows worse with n.

  18. Quantification of noise sources for amperometric measurement of quantal exocytosis using microelectrodes

    PubMed Central

    Yao, Jia

    2016-01-01

    Electrochemical microelectrodes are commonly used to record amperometric spikes of current that result from oxidation of transmitter released from individual vesicles during exocytosis. Whereas the exquisite sensitivity of these measurements is well appreciated, a better understanding of the noise sources that limit the resolution of the technique is needed to guide the design of next-generation devices. We measured the current power spectral density (SI) of electrochemical microelectrodes to understand the physical basis of dominant noise sources and to determine how noise varies with the electrode material and geometry. We find that the current noise is thermal in origin in that SI is proportional to the real part of the admittance of the electrode. The admittance of microelectrodes is well described by a constant phase element model such that both the real and imaginary admittance increase with frequency raised to a power of 0.84 – 0.96. Our results demonstrate that the current standard deviation is proportional to the square root of the area of the working electrode, increases ~linearly with the bandwidth of the recording, and varies with the choice of the electrode material with Au ≈ carbon fiber > nitrogen-doped diamond-like carbon > indium-tin-oxide. Contact between a cell and a microelectrode does not appreciably increase noise. Surface-patterned microchip electrodes can have a noise performance that is superior to that of carbon-fiber microelectrodes of the same area. PMID:22540116

  19. Asynchronous Cooperative e-Learning System and Its Evaluation

    NASA Astrophysics Data System (ADS)

    Eto, Kazuhiro; Miyoshi, Takumi

    Much attention has been attracted to collaborative learning on an e-learning system. However, it is difficult to implement the collaborative environment to an asynchronous e-learning system since collaboration would be realized only when learners join the system at the same time. On the other hand, cooperative learning has been proposed. In this method, learners can study on their own pace without making mutual agreement but with receiving cognitive information from others. In this paper, the authors have developed the asynchronous cooperative e-learning system that provides learners' attendance and studying progress as the cognitive information. The subjective evaluation experiments show that our system is slightly inferior to the synchronous collaborative e-learning system, but it can motivate the learners more than the conventional system.

  20. Asynchronous Message Service for Deep Space Mission Operations

    NASA Technical Reports Server (NTRS)

    Burleigh, Scott C.

    2006-01-01

    While the CCSDS (Consultative Committee for Space Data Systems) File Delivery Protocol (CFDP) provides internationally standardized file transfer functionality that can offer significant benefits for deep space mission operations, not all spacecraft communication requirements are necessarily best met by file transfer. In particular, continuous event-driven asynchronous message exchange may also be useful for communications with, among, and aboard spacecraft. CCSDS has therefore undertaken the development of a new Asynchronous Message Service (AMS) standard, designed to provide common functionality over a wide variety of underlying transport services, ranging from shared memory message queues to CCSDS telemetry systems. The present paper discusses the design concepts of AMS, their applicability to deep space mission operations problems, and the results of preliminary performance testing obtained from exercise of a prototype implementation.

  1. Asynchronous emergence by loggerhead turtle (Caretta caretta) hatchlings

    NASA Astrophysics Data System (ADS)

    Houghton, J. D. R.; Hays, G. C.

    2001-03-01

    For many decades it has been accepted that marine turtle hatchlings from the same nest generally emerge from the sand together. However, for loggerhead turtles (Caretta caretta) nesting on the Greek Island of Kefalonia, a more asynchronous pattern of emergence has been documented. By placing temperature loggers at the top and bottom of nests laid on Kefalonia during 1998, we examined whether this asynchronous emergence was related to the thermal conditions within nests. Pronounced thermal variation existed not only between, but also within, individual nests. These within-nest temperature differences were related to the patterns of hatchling emergence, with hatchlings from nests displaying large thermal ranges emerging over a longer time-scale than those characterised by more uniform temperatures.

  2. Parallel, Asynchronous Executive (PAX): System concepts, facilities, and architecture

    NASA Technical Reports Server (NTRS)

    Jones, W. H.

    1983-01-01

    The Parallel, Asynchronous Executive (PAX) is a software operating system simulation that allows many computers to work on a single problem at the same time. PAX is currently implemented on a UNIVAC 1100/42 computer system. Independent UNIVAC runstreams are used to simulate independent computers. Data are shared among independent UNIVAC runstreams through shared mass-storage files. PAX has achieved the following: (1) applied several computing processes simultaneously to a single, logically unified problem; (2) resolved most parallel processor conflicts by careful work assignment; (3) resolved by means of worker requests to PAX all conflicts not resolved by work assignment; (4) provided fault isolation and recovery mechanisms to meet the problems of an actual parallel, asynchronous processing machine. Additionally, one real-life problem has been constructed for the PAX environment. This is CASPER, a collection of aerodynamic and structural dynamic problem simulation routines. CASPER is not discussed in this report except to provide examples of parallel-processing techniques.

  3. Asynchronous sequential circuit design using pass transistor iterative logic arrays

    NASA Technical Reports Server (NTRS)

    Liu, M. N.; Maki, G. K.; Whitaker, S. R.

    1991-01-01

    The iterative logic array (ILA) is introduced as a new architecture for asynchronous sequential circuits. This is the first ILA architecture for sequential circuits reported in the literature. The ILA architecture produces a very regular circuit structure. Moreover, it is immune to both 1-1 and 0-0 crossovers and is free of hazards. This paper also presents a new critical race free STT state assignment which produces a simple form of design equations that greatly simplifies the ILA realizations.

  4. Asynchronous Transfer Mode (ATM) Switch Technology and Vendor Survey

    NASA Technical Reports Server (NTRS)

    Berry, Noemi

    1995-01-01

    Asynchronous Transfer Mode (ATM) switch and software features are described and compared in order to make switch comparisons meaningful. An ATM switch's performance cannot be measured solely based on its claimed switching capacity; traffic management and congestion control are emerging as the determining factors in an ATM network's ultimate throughput. Non-switch ATM products and experiences with actual installations of ATM networks are described. A compilation of select vendor offerings as of October 1994 is provided in chart form.

  5. Non-Detection of Nova Shells Around Asynchronous Polars

    NASA Astrophysics Data System (ADS)

    Pagnotta, Ashley; Zurek, David

    2016-01-01

    Asynchronous polars (APs) are accreting white dwarfs (WDs) that have different WD and orbital angular velocities, unlike the rest of the known polars, which rotate synchronously (i.e., their WD and orbital angular velocities are the same). Past nova eruptions are the predicted cause of the asynchronicity, in part due to the fact that one of the APs, V1500 Cyg, was observed to undergo a nova eruption in 1975. We used the SALT 10m-class telescope and the MDM 2.4m Hiltner telescope to search for nova shells around three of the remaining four APs (V1432 Aql, BY Cam, and CD Ind) as well as one Intermediate Polar with a high asynchronicity (EX Hya). We found no evidence of nova shells in any of our images. We therefore cannot say that any of the systems besides V1500 Cyg had nova eruptions, but because not all post-nova systems have detectable shells, we also cannot exclude the possibility of a nova eruption occurring in any of these systems and knocking the rotation out of sync.

  6. Non-detection of nova shells around asynchronous polars

    NASA Astrophysics Data System (ADS)

    Pagnotta, Ashley; Zurek, David

    2016-05-01

    Asynchronous polars (APs) are accreting white dwarfs (WDs) that have different WD and orbital angular velocities, unlike the rest of the known polars, which rotate synchronously (i.e. their WD and orbital angular velocities are the same). Past nova eruptions are the predicted cause of the asynchronicity, in part due to the fact that one of the APs, V1500 Cyg, was observed to undergo a nova eruption in 1975. We used the Southern African Large Telescope 10 m class telescope and the MDM 2.4 m Hiltner telescope to search for nova shells around three of the remaining four APs (V1432 Aql, BY Cam, and CD Ind) as well as one Intermediate Polar with a high asynchronicity (EX Hya). We found no evidence of nova shells in any of our images. We therefore cannot say that any of the systems besides V1500 Cyg had nova eruptions, but because not all post-nova systems have detectable shells, we also cannot exclude the possibility of a nova eruption occurring in any of these systems and knocking the rotation out of sync.

  7. Data Collection for Mobile Group Consumption: An Asynchronous Distributed Approach.

    PubMed

    Zhu, Weiping; Chen, Weiran; Hu, Zhejie; Li, Zuoyou; Liang, Yue; Chen, Jiaojiao

    2016-01-01

    Mobile group consumption refers to consumption by a group of people, such as a couple, a family, colleagues and friends, based on mobile communications. It differs from consumption only involving individuals, because of the complex relations among group members. Existing data collection systems for mobile group consumption are centralized, which has the disadvantages of being a performance bottleneck, having single-point failure and increasing business and security risks. Moreover, these data collection systems are based on a synchronized clock, which is often unrealistic because of hardware constraints, privacy concerns or synchronization cost. In this paper, we propose the first asynchronous distributed approach to collecting data generated by mobile group consumption. We formally built a system model thereof based on asynchronous distributed communication. We then designed a simulation system for the model for which we propose a three-layer solution framework. After that, we describe how to detect the causality relation of two/three gathering events that happened in the system based on the collected data. Various definitions of causality relations based on asynchronous distributed communication are supported. Extensive simulation results show that the proposed approach is effective for data collection relating to mobile group consumption. PMID:27058544

  8. A Synchronous-Asynchronous Particle Swarm Optimisation Algorithm

    PubMed Central

    Ab Aziz, Nor Azlina; Mubin, Marizan; Mohamad, Mohd Saberi; Ab Aziz, Kamarulzaman

    2014-01-01

    In the original particle swarm optimisation (PSO) algorithm, the particles' velocities and positions are updated after the whole swarm performance is evaluated. This algorithm is also known as synchronous PSO (S-PSO). The strength of this update method is in the exploitation of the information. Asynchronous update PSO (A-PSO) has been proposed as an alternative to S-PSO. A particle in A-PSO updates its velocity and position as soon as its own performance has been evaluated. Hence, particles are updated using partial information, leading to stronger exploration. In this paper, we attempt to improve PSO by merging both update methods to utilise the strengths of both methods. The proposed synchronous-asynchronous PSO (SA-PSO) algorithm divides the particles into smaller groups. The best member of a group and the swarm's best are chosen to lead the search. Members within a group are updated synchronously, while the groups themselves are asynchronously updated. Five well-known unimodal functions, four multimodal functions, and a real world optimisation problem are used to study the performance of SA-PSO, which is compared with the performances of S-PSO and A-PSO. The results are statistically analysed and show that the proposed SA-PSO has performed consistently well. PMID:25121109

  9. Divorce and asynchronous arrival in common terns, Sterna hirundo.

    PubMed

    GonzáLez-SolíS; Becker; Wendeln

    1999-11-01

    We investigated which of three hypotheses (better option, incompatibility or asynchronous arrival) best explains divorce in the common tern. One partner did not return the next year in 18.5% of 150 pairs. Among the 106 pairs in which both mates returned, the divorce rate was 18.9%. We found no significant differences in: breeding performance or condition in relation to the probability of divorce; quality of previous mates and new mates, mean age in relation to pair bond status; breeding success before and after divorce nor did this differ from breeding success of reunited pairs. Hence the better option and incompatibility hypotheses were not supported. However, divorce was more likely in pairs in which mates arrived asynchronously on the breeding grounds, supporting the asynchronous arrival hypothesis. Median arrival asynchrony for divorced pairs was 7.5 days and for reunited pairs 2 days; mates arriving more than 16 days apart always split up. About 20% of divorced birds lost breeding status in the year of divorce, probably as a consequence of their late arrival. Our results suggest that terns search for a new mate as soon as they arrive on the breeding grounds and that mates remain faithful to each other to avoid the costs of searching for a new partner. Thus, synchrony in arrival facilitates pair bond maintenance rather than asynchrony promoting divorce, since divorce appears to be a side-effect of asynchrony and not an active decision. Copyright 1999 The Association for the Study of Animal Behaviour. PMID:10564616

  10. Asynchronous hatching and food limitation: a test of Lack's hypothesis

    USGS Publications Warehouse

    Skagen, Susan Knight

    1988-01-01

    Lack’s (1954, 1968) hypothesis that asynchronous hatching of altricial birds is an adaptive response to unpredictable food shortages during the breeding season was examined in the highly granivorous Zebra Finch (Poephila guttata). I compared growth and survival of nestlings in asynchronous and artificially created synchronous brood reared under food-limited and food-abundant conditions in an aviary. I also examined the role of parental experience on survival and growth of nestlings. There was no differential mortality of Zebra Finch nestlings due to either asynchrony or food abundance. Young in abundant food treatments grew more rapidly, however, than those in food restricted treatments. Heaviest Zebra Finch nestlings in a brood grew more quickly than their lightest siblings when food was limited, supporting Lack’s hypothesis. Further, differential survival of light and heavy siblings occurred when food was abundant, suggesting that asynchronous hatching can be maladaptive under some ecological conditions. Nestlings reared by inexperienced parents suffered greater mortality and slower growth when food was abundant than nestlings raised by experienced parents. Prefledging mass was correlated with size at adulthood.

  11. Robust Read Channel System Directly Processing Asynchronous Sampling Data

    NASA Astrophysics Data System (ADS)

    Yamamoto, Akira; Mouri, Hiroki; Yamamoto, Takashi

    2006-02-01

    In this study, we describe a robust read channel employing a novel timing recovery system and a unique Viterbi detector which extracts channel timing and channel data directly from asynchronous sampling data. The timing recovery system in the proposed read channel has feed-forward architecture and consists entirely of digital circuits. Thus, it enables robust timing recovery at high-speed and has no performance deterioration caused by variations in analog circuits. The Viterbi detector not only detects maximum-likelihood data using a reference level generator, but also transforms asynchronous data into pseudosynchronous data using two clocks, such as an asynchronous clock generated by a frequency synthesizer and a pseudosynchronous clock generated by a timing detector. The proposed read channel has achieved a constant and fast frequency acquisition time against initial frequency error and has improved its bit error rate performance. This robust read channel system can be used for high-speed signal processing and LSIs using nanometer-scale semiconductor processes.

  12. Asynchronous Stoichiometric Response in Lithium Iron Phosphate Batteries

    SciTech Connect

    Paxton, William A.; Akdogan, E. Koray; Savkliyidiz, Ilyas; Choksi, Ankur U.; Silver, Scott X.; Tsakalokos, Thomas; Zhong, Zhong

    2014-11-11

    The operando energy-dispersive x-ray diffraction (EDXRD) was carried out on a newly formed 8 Ah lithium iron phosphate (LiFePO4) battery with the goal of elucidating the origin of asynchronous phase transformation commonly seen with in situ x-ray diffraction studies. The high-energy photons at the NSLS X17B1 beamline allow for penetration into a fully assembled battery and therefore negate any need for a specially designed in situ cell which often uses modified current collectors to minimize x-ray attenuation. Spatially-and-temporally resolved phase-mapping was conducted with a semiquantitative reference intensity ratio (RIR) analysis to estimate the relative abundance of the delithiated phase. The data show an asynchronous response in the stoichiometry versus the electrochemical profile and suggest limited diffusion in the electrode toward the end of discharge. These results confirm that the asynchronous electrode response is not just limited to specially designed cells but occurs in fully assembled cells alike. We attribute this behavior to be a consequence of performing a local measurement over a wide-area heterogeneous reaction.

  13. Pharmacists’ Perception of Synchronous Versus Asynchronous Distance Learning for Continuing Education Programs

    PubMed Central

    2014-01-01

    Objective. To evaluate and compare pharmacists’ satisfaction with the content and learning environment of a continuing education program series offered as either synchronous or asynchronous webinars. Methods. An 8-lecture series of online presentations on the topic of new drug therapies was offered to pharmacists in synchronous and asynchronous webinar formats. Participants completed a 50-question online survey at the end of the program series to evaluate their perceptions of the distance learning experience. Results. Eighty-two participants completed the survey instrument (41 participants from the live webinar series and 41 participants from the asynchronous webinar series.) Responses indicated that while both groups were satisfied with the program content, the asynchronous group showed greater satisfaction with many aspects of the learning environment. Conclusion. The synchronous and asynchronous webinar participants responded positively regarding the quality of the programming and the method of delivery, but asynchronous participants rated their experience more positively overall. PMID:24558276

  14. The upregulation of acetylcholine release at endplates of alpha-bungarotoxin-treated rats: its dependency on calcium.

    PubMed Central

    Plomp, J J; van Kempen, G T; Molenaar, P C

    1994-01-01

    1. The presynaptic component of an adaptive feedback mechanism leading to increased acetylcholine (ACh) release was studied in endplates of diaphragms from rats treated chronically with alpha-bungarotoxin (alpha BTX). 2. Quantal contents were calculated 'directly' from the amplitude of miniature endplate potentials (MEPPs) and endplate potentials (EPPs) which were recorded after mu-conotoxin treatment to prevent muscle action potentials. 3. In vitro application of the Ca2+ channel blockers nifedipine (10 microM) or omega-conotoxin (40 nM) had no significant effect on the increased quantal content of endplates from alpha BTX-treated rats. 4. At control endplates, in vitro block of presynaptic K+ channels by 5 microM 3,4-diaminopyridine did increase the quantal content to a level which was similar to that found in endplates of alpha BTX-treated rats but also induced a broadening of EPPs, which was not found at endplates after alpha BTX treatment. 5. The difference between quantal contents of alpha BTX-treated and control rats was highly dependent on the [Ca2+]o/[Mg2+]o ratio when [Mg2+]o was fixed at 1 mM. At low [Ca2+]o, the quantal content of endplates from alpha BTX-treated rats was lower than that of controls while at [Ca2+]o in the normal and high range this was reversed. However, changing the [Ca2+]o/[Mg2+]o ratio by means of [Mg2+]o, at a fixed [Ca2+]o of 2 mM, did not influence the relative increase of quantal contents at endplates from alpha BTX-treated rats. Double logarithmic plots of the 'toxin-induced' myasthenia gravis (TIMG) and control quantal content versus [Ca2+]o had an approximately linear part between 0.2 and 1.5 mM [Ca2+]o. The slopes of the TIMG and control lines were 1.81 and 0.96, indicating that the ACh release in TIMG muscles was more sensitive to changes of [Ca2+]o than controls. 6. At normal [Ca2+]o and [Mg2+]o, the depression of EPP amplitude during stimulation of the phrenic nerve at 30-50 Hz was somewhat larger at endplates from alpha

  15. The Effects of Sensorineural Hearing Impairment on Asynchronous Glimpsing of Speech

    PubMed Central

    Buss, Emily; Hall, Joseph W.

    2016-01-01

    In a previous study with normal-hearing listeners, we evaluated consonant identification masked by two or more spectrally contiguous bands of noise, with asynchronous square-wave modulation applied to neighboring bands. Speech recognition thresholds were 5.1–8.5 dB better when neighboring bands were presented to different ears (dichotic) than when all bands were presented to one ear (monaural), depending on the spectral width of the frequency bands. This dichotic advantage was interpreted as reflecting masking release from peripheral spread of masking from neighboring frequency bands. The present study evaluated this effect in listeners with sensorineural hearing loss, a population more susceptible to spread of masking. Speech perception (vowel-consonant-vowel stimuli, as in /aBa/) was measured in the presence of fluctuating noise that was either modulated synchronously across frequency or asynchronously. Hearing-impaired listeners (n = 9) and normal-hearing controls were tested at either the same intensity (n = 7) or same sensation level (n = 8). Hearing-impaired listeners had mild-to-moderate hearing loss and symmetrical, flat audiometric thresholds. While all groups of listeners performed better in the dichotic than monaural condition, this effect was smaller for the hearing-impaired (3.5 dB) and equivalent-sensation-level controls (3.3 dB) than controls tested at the same intensity (11.0 dB). The present study is consistent with the idea that dichotic presentation can improve speech-in-noise listening for hearing-impaired listeners, and may be enhanced when combined with amplification. PMID:27144601

  16. Inorganic and organic mercury chloride toxicity to Coturnix: Sensitivity related to age and quantal assessment of physiologic responses

    USGS Publications Warehouse

    Hill, E.F.

    1982-01-01

    The toxicities of mercuric chloride (HgCl(,2)) and methylmercuric chloride (CH(,3)HgCl) were compared for coturnix (Coturnix coturnix japonica) from hatching to adulthood. Comparisons were based on: (1) Median lethal dosages (LD50) derived by administering single peroral and single intramuscular dosages of mercury, (2) median lethal concentrations (LC50) derived by feeding mercury for 5 days, (3) median toxic concentrations (TC50) derived by feeding mercury 9 weeks and measuring plasma enzyme activity, plasma electrolytes, and other blood constituents, and (4) transient changes of various blood chemistries following a single peroral dose of mercury. Acute peroral and intramuscular LD50s for HgCl(,2) and CH(,3)HgCl increased by two- to threefold for coturnix chicks from hatching to 4 weeks of age. Concomitantly, the LC50s also increased, but the important difference between test procedures was that with both single dose routes of exposure the toxicity ratios, i.e., HgCl(,2)/CH(,3)HgCl, at each age were about 2 to 2.5 compared to about 100 for the LC50s. For example, at 2 weeks of age the peroral LD50s for HgCl(,2) and CH(,3)HgCl were 42 and 18 mg/kg; the dietary LC50s were 5086 and 47 ppm for HgCl(,2) and CH(,3)HgCl. The 9 week feeding trial was not associated with gross effects from either HgCl(,2) at 0.5 to 32 ppm or CH(,3)HgCl at 0.125 to 8 ppm. However, subtle responses were detected for the plasma enzymes aspartate aminotransferase, lactate dehydrogenase, and ornithine carbamoyl transferase and could be quantified by probit analysis. This quantal procedure was based on establishment of a normal value for each enzyme and classing outliers as respondents. A 'hazard index' based on the TC50 for an enzyme divided by the LD50 or LC50 was introduced. The single oral dosages of HgCl(,2) and CH(,3)HgCl showed that ratios of alanine aminotransferase, lactate dehydrogenase, and orinthine carbamoyl transferase for the liver and kidneys of adult coturnix were opposite from

  17. Asynchronous Laser Transponders for Precise Interplanetary Ranging and Time Transfer

    NASA Technical Reports Server (NTRS)

    Degnan, John J.; Smith, David E. (Technical Monitor)

    2001-01-01

    The feasibility of a two-way asynchronous (i.e. independently firing) interplanetary laser transponder pair, capable of decimeter ranging and subnanosecond time transfer from Earth to a spacecraft anywhere within the inner Solar System, is discussed. In the Introduction, we briefly discuss the current state-of-the-art in Satellite Laser Ranging (SLR) and Lunar Laser Ranging (LLR) which use single-ended range measurements to a passive optical reflector, and the limitations of this approach in ranging beyond the Moon to the planets. In Section 2 of this paper, we describe two types of transponders (echo and asynchronous), introduce the transponder link equation and the concept of "balanced" transponders, describe how range and time can be transferred between terminals, and preview the potential advantages of photon counting asynchronous transponders for interplanetary applications. In Section 3, we discuss and provide mathematical models for the various sources of noise in an interplanetary transponder link including planetary albedo, solar or lunar illumination of the local atmosphere, and laser backscatter off the local atmosphere. In Section 4, we introduce the key engineering elements of an interplanetary laser transponder and develop an operational scenario for the acquisition and tracking of the opposite terminal. In Section 5, we use the theoretical models of th previous sections to perform an Earth-Mars link analysis over a full synodic period of 780 days under the simplifying assumption of coaxial, coplanar, circular orbits. We demonstrate that, using slightly modified versions of existing space and ground based laser systems, an Earth-Mars transponder link is not only feasible but quite robust. We also demonstrate through analysis the advantages and feasibility of compact, low output power (<300 mW photon-counting transponders using NASA's developmental SLR2000 satellite laser ranging system as the Earth terminal. Section 6 provides a summary of the results

  18. RAPID COMMUNICATION: Asynchronous data-driven classification of weapon systems

    NASA Astrophysics Data System (ADS)

    Jin, Xin; Mukherjee, Kushal; Gupta, Shalabh; Ray, Asok; Phoha, Shashi; Damarla, Thyagaraju

    2009-12-01

    This communication addresses real-time weapon classification by analysis of asynchronous acoustic data, collected from microphones on a sensor network. The weapon classification algorithm consists of two parts: (i) feature extraction from time-series data using symbolic dynamic filtering (SDF), and (ii) pattern classification based on the extracted features using the language measure (LM) and support vector machine (SVM). The proposed algorithm has been tested on field data, generated by firing of two types of rifles. The results of analysis demonstrate high accuracy and fast execution of the pattern classification algorithm with low memory requirements. Potential applications include simultaneous shooter localization and weapon classification with soldier-wearable networked sensors.

  19. Entry of Ebola Virus is an Asynchronous Process.

    PubMed

    Reynard, Olivier; Volchkov, Viktor E

    2015-10-01

    Ebola virus (EBOV) is responsible for a severe fever with a high mortality rate. The diverse nature of the attachment of the virus to the cell surface, the initial step of virus entry, raises questions concerning the kinetics of the virus internalization process. We investigated EBOV entry kinetics using the activity of a particular monoclonal antibody that neutralizes virus infectivity. We demonstrate that inoculation of cells with EBOV results in an asynchronous entry process, as revealed by the ability of the virus to remain in a cell-bound state for an extended period of time before it is internalized. PMID:25941332

  20. A wavelet approach to binary blackholes with asynchronous multitasking

    NASA Astrophysics Data System (ADS)

    Lim, Hyun; Hirschmann, Eric; Neilsen, David; Anderson, Matthew; Debuhr, Jackson; Zhang, Bo

    2016-03-01

    Highly accurate simulations of binary black holes and neutron stars are needed to address a variety of interesting problems in relativistic astrophysics. We present a new method for the solving the Einstein equations (BSSN formulation) using iterated interpolating wavelets. Wavelet coefficients provide a direct measure of the local approximation error for the solution and place collocation points that naturally adapt to features of the solution. Further, they exhibit exponential convergence on unevenly spaced collection points. The parallel implementation of the wavelet simulation framework presented here deviates from conventional practice in combining multi-threading with a form of message-driven computation sometimes referred to as asynchronous multitasking.

  1. The elusive quantal individual

    NASA Astrophysics Data System (ADS)

    Groenewold, H. J.

    1985-10-01

    In the formal hedgehog representation of quantum mechanics [5] (ambiguous) weights are derived for hedgehogs with a finite number of questions and answers, in particular applied to spin {1}/{2} and to correlated spin {1}/{2} pairs. Unavoidable negative weights are a clear signal for conceptual difficulties in quantum mechanical interpretation. If these weights had been presupposed to be non-negative, they could have led to Bell-like inequalities inconsistent with quantum mechanics. This is what has happened already in various special models. Owing to the indefinite weights, the hedgehog hypothesis of one-to-one mapping between individual physical samples and individual fictitious hedgehogs cannot be maintained. If no physical interpretation is conceived for the negative weights, the only way to avoid unsolved conceptual difficulties appears to resign (even in the hedgehog representation) to the skeptical ensemble interpretation [1], without theorizing about individual physical samples at all.

  2. Mechanisms, pools, and sites of spontaneous vesicle release at synapses of rod and cone photoreceptors.

    PubMed

    Cork, Karlene M; Van Hook, Matthew J; Thoreson, Wallace B

    2016-08-01

    Photoreceptors have depolarized resting potentials that stimulate calcium-dependent release continuously from a large vesicle pool but neurons can also release vesicles without stimulation. We characterized the Ca(2+) dependence, vesicle pools, and release sites involved in spontaneous release at photoreceptor ribbon synapses. In whole-cell recordings from light-adapted horizontal cells (HCs) of tiger salamander retina, we detected miniature excitatory post-synaptic currents (mEPSCs) when no stimulation was applied to promote exocytosis. Blocking Ca(2+) influx by lowering extracellular Ca(2+) , by application of Cd(2+) and other agents reduced the frequency of mEPSCs but did not eliminate them, indicating that mEPSCs can occur independently of Ca(2+) . We also measured release presynaptically from rods and cones by examining quantal glutamate transporter anion currents. Presynaptic quantal event frequency was reduced by Cd(2+) or by increased intracellular Ca(2+) buffering in rods, but not in cones, that were voltage clamped at -70 mV. By inhibiting the vesicle cycle with bafilomycin, we found the frequency of mEPSCs declined more rapidly than the amplitude of evoked excitatory post-synaptic currents (EPSCs) suggesting a possible separation between vesicle pools in evoked and spontaneous exocytosis. We mapped sites of Ca(2+) -independent release using total internal reflectance fluorescence (TIRF) microscopy to visualize fusion of individual vesicles loaded with dextran-conjugated pHrodo. Spontaneous release in rods occurred more frequently at non-ribbon sites than evoked release events. The function of Ca(2+) -independent spontaneous release at continuously active photoreceptor synapses remains unclear, but the low frequency of spontaneous quanta limits their impact on noise. PMID:27255664

  3. Asynchronous Discussion Board Facilitation and Rubric Use in a Blended Learning Environment

    ERIC Educational Resources Information Center

    Giacumo, Lisa

    2012-01-01

    The purpose of this study was to investigate the effects of instructor response prompts and rubrics on students' performance in an asynchronous discussion-board assignment, their learning achievement on an objective-type posttest, and their reported satisfaction levels. Researchers who have studied asynchronous computer-mediated student…

  4. Combining Live Video and Audio Broadcasting, Synchronous Chat, and Asynchronous Open Forum Discussions in Distance Education

    ERIC Educational Resources Information Center

    Teng, Tian-Lih; Taveras, Marypat

    2004-01-01

    This article outlines the evolution of a unique distance education program that began as a hybrid--combining face-to-face instruction with asynchronous online teaching--and evolved to become an innovative combination of synchronous education using live streaming video, audio, and chat over the Internet, blended with asynchronous online discussions…

  5. Fostering Critical Reflection in a Computer-Based, Asynchronously Delivered Diversity Training Course

    ERIC Educational Resources Information Center

    Givhan, Shawn T.

    2013-01-01

    This dissertation study chronicles the creation of a computer-based, asynchronously delivered diversity training course for a state agency. The course format enabled efficient delivery of a mandatory curriculum to the Massachusetts Department of State Police workforce. However, the asynchronous format posed a challenge to achieving the learning…

  6. Designing Asynchronous, Text-Based Computer Conferences: Ten Research-Based Suggestions

    ERIC Educational Resources Information Center

    Choitz, Paul; Lee, Doris

    2006-01-01

    Asynchronous computer conferencing refers to the use of computer software and a network enabling participants to post messages that allow discourse to continue even though interactions may be extended over days and weeks. Asynchronous conferences are time-independent, adapting to multiple time zones and learner schedules. Such activities as…

  7. Using Television Sitcoms to Facilitate Asynchronous Discussions in the Online Communication Course

    ERIC Educational Resources Information Center

    Tolman, Elizabeth; Asbury, Bryan

    2012-01-01

    Asynchronous discussions are a useful instructional resource in the online communication course. In discussion groups students have the opportunity to actively participate and interact with students and the instructor. Asynchronous communication allows for flexibility because "participants can interact with significant amounts of time between…

  8. Anonymity and Motivation in Asynchronous Discussions and L2 Vocabulary Learning

    ERIC Educational Resources Information Center

    Polat, Nihat; Mancilla, Rae; Mahalingappa, Laura

    2013-01-01

    This study investigates L2 attainment in asynchronous online environments, specifically possible relationships among anonymity, L2 motivation, participation in discussions, quality of L2 production, and success in L2 vocabulary learning. It examines, in asynchronous discussions, (a) if participation and (b) motivation contribute to L2 vocabulary…

  9. An Investigation of the Impact of Asynchronous Online Learning on Student Achievement

    ERIC Educational Resources Information Center

    Hill, Sylvia

    2009-01-01

    Asynchronous online learning is well documented with adults, but fewer studies of effectiveness have emphasized elementary education learning environments. This study investigated student achievement on the Texas Assessment of Knowledge and Skills (TAKS) test for fifth grade students who had participated in synchronous and asynchronous courses…

  10. Designing Asynchronous Online Discussion Environments: Recent Progress and Possible Future Directions

    ERIC Educational Resources Information Center

    Gao, Fei; Zhang, Tianyi; Franklin, Teresa

    2013-01-01

    Asynchronous online discussion environments are important platforms to support learning. Research suggests, however, threaded forums, one of the most popular asynchronous discussion environments, do not often foster productive online discussions naturally. This paper explores how certain properties of threaded forums have affected or constrained…

  11. The Role of Offline Metalanguage Talk in Asynchronous Computer-Mediated Communication

    ERIC Educational Resources Information Center

    Kitade, Keiko

    2008-01-01

    In order to demonstrate how learners utilize the text-based asynchronous attributes of the Bulletin Board System, this study explored Japanese-as-a-second-language learners' metalanguage episodes (Swain & Lapkin, 1995, 1998) in offline verbal peer speech and online asynchronous discussions with their Japanese key pals. The findings suggest the…

  12. Elements that Impact Facilitation of an Asynchronous Professional Learning Community: A Participatory Action Research Exploration

    ERIC Educational Resources Information Center

    Hofer, Marilyn

    2009-01-01

    Despite that fact that the demand for asynchronous learning is booming, the literature on distance learning facilitation skills is lacking (Sherry, 1996). This study supports the premise that the use of paraphrasing strategies impacts learners in an asynchronous environment. The research question has been narrowed to inspect the particular…

  13. Presynaptic effects of d-tubocurarine on neurotransmitter release at the neuromuscular junction of the frog.

    PubMed Central

    Matzner, H; Parnas, H; Parnas, I

    1988-01-01

    1. Presynaptic effects of d-tubocurarine on neurotransmitter release were examined at the frog neuromuscular junction, using intracellular and extracellular recording techniques. 2. d-Tubocurarine in concentrations of 10(-7)-10(-6) M decreased the quantal content (m) measured by the coefficient of variation and failure methods. 3. d-Tubocurarine produced a shift to the right of the curve relating log quantal content to log [Ca2+]o without changing the slope. 4. The duration of twin-impulse facilitation was not affected by 5 x 10(-7) M-d-tubocurarine. Early facilitation was higher in d-tubocurarine. 5. d-Tubocurarine altered the synaptic delay histogram. The peak of the histogram was shifted to longer delays. Prolongation of the minimal delay was seen in most but not all experiments. 6. These results suggest that d-tubocurarine inhibits release of neurotransmitter by affecting a stage in the process of release, which occurs after the entry of Ca2+ ions. PMID:2899171

  14. Designing Asynchronous Communication Tools for Optimization of Patient-Clinician Coordination

    PubMed Central

    Eschler, Jordan; Liu, Leslie S.; Vizer, Lisa M.; McClure, Jennifer B.; Lozano, Paula; Pratt, Wanda; Ralston, James D.

    2015-01-01

    Asynchronous communication outside the clinical setting has both enriched and complicated patient-clinician interactions. Many patients can now interact with a patient portal 24 hours a day, asking questions of their clinicians via secure message, checking lab results, ordering medication refills, or making appointments. However, the mode of communication (asynchronous) and the nature of the interaction (lacking tone or body language) strip valuable information from each side of patient-clinician asynchronous communication. Using interviews with 34 individuals who actively manage a chronic illness of their own, or for a child or partner, we elicited narratives about patients’ experiences and expectations for using asynchronous communication to address medical issues with their clinicians. Based on these perspectives, we present opportunities for designing asynchronous communication tools to better facilitate understanding of and coordination around care activities between patients and clinicians. PMID:26958188

  15. Asynchronous Processing of a Constellation of Geostationary and Polar-Orbiting Satellites for Fire Detection and Smoke Estimation

    NASA Astrophysics Data System (ADS)

    Hyer, E. J.; Peterson, D. A.; Curtis, C. A.; Schmidt, C. C.; Hoffman, J.; Prins, E. M.

    2014-12-01

    The Fire Locating and Monitoring of Burning Emissions (FLAMBE) system converts satellite observations of thermally anomalous pixels into spatially and temporally continuous estimates of smoke release from open biomass burning. This system currently processes data from a constellation of 5 geostationary and 2 polar-orbiting sensors. Additional sensors, including NPP VIIRS and the imager on the Korea COMS-1 geostationary satellite, will soon be added. This constellation experiences schedule changes and outages of various durations, making the set of available scenes for fire detection highly variable on an hourly and daily basis. Adding to the complexity, the latency of the satellite data is variable between and within sensors. FLAMBE shares with many fire detection systems the goal of detecting as many fires as possible as early as possible, but the FLAMBE system must also produce a consistent estimate of smoke production with minimal artifacts from the changing constellation. To achieve this, NRL has developed a system of asynchronous processing and cross-calibration that permits satellite data to be used as it arrives, while preserving the consistency of the smoke emission estimates. This talk describes the asynchronous data ingest methodology, including latency statistics for the constellation. We also provide an overview and show results from the system we have developed to normalize multi-sensor fire detection for consistency.

  16. Brain-derived neurotrophic factor enhances GABA release probability and nonuniform distribution of N- and P/Q-type channels on release sites of hippocampal inhibitory synapses.

    PubMed

    Baldelli, Pietro; Hernandez-Guijo, Jesus-Miguel; Carabelli, Valentina; Carbone, Emilio

    2005-03-30

    Long-lasting exposures to brain-derived neurotrophic factor (BDNF) accelerate the functional maturation of GABAergic transmission in embryonic hippocampal neurons, but the molecular bases of this phenomenon are still debated. Evidence in favor of a postsynaptic site of action has been accumulated, but most of the data support a presynaptic site effect. A crucial issue is whether the enhancement of evoked IPSCs (eIPSCs) induced by BDNF is attributable to an increase in any of the elementary parameters controlling neurosecretion, namely the probability of release, the number of release sites, the readily releasable pool (RRP), and the quantal size. Here, using peak-scaled variance analysis of miniature IPSCs, multiple probability fluctuation analysis, and cumulative amplitude analysis of action potential-evoked postsynaptic currents, we show that BDNF increases release probability and vesicle replenishment with little or no effect on the quantal size, the number of release sites, the RRP, and the Ca2+ dependence of eIPSCs. BDNF treatment changes markedly the distribution of Ca2+ channels controlling neurotransmitter release. It enhances markedly the contribution of N- and P/Q-type channels, which summed to >100% ("supra-additivity"), and deletes the contribution of R-type channels. BDNF accelerates the switch of presynaptic Ca2+ channel distribution from "segregated" to "nonuniform" distribution. This maturation effect was accompanied by an uncovered increased control of N-type channels on paired-pulse depression, otherwise dominated by P/Q-type channels in untreated neurons. Nevertheless, BDNF preserved the fast recovery from depression associated with N-type channels. These novel presynaptic BDNF actions derive mostly from an enhanced overlapping and better colocalization of N- and P/Q-type channels to vesicle release sites. PMID:15800191

  17. Parallel asynchronous hardware implementation of image processing algorithms

    NASA Technical Reports Server (NTRS)

    Coon, Darryl D.; Perera, A. G. U.

    1990-01-01

    Research is being carried out on hardware for a new approach to focal plane processing. The hardware involves silicon injection mode devices. These devices provide a natural basis for parallel asynchronous focal plane image preprocessing. The simplicity and novel properties of the devices would permit an independent analog processing channel to be dedicated to every pixel. A laminar architecture built from arrays of the devices would form a two-dimensional (2-D) array processor with a 2-D array of inputs located directly behind a focal plane detector array. A 2-D image data stream would propagate in neuron-like asynchronous pulse-coded form through the laminar processor. No multiplexing, digitization, or serial processing would occur in the preprocessing state. High performance is expected, based on pulse coding of input currents down to one picoampere with noise referred to input of about 10 femtoamperes. Linear pulse coding has been observed for input currents ranging up to seven orders of magnitude. Low power requirements suggest utility in space and in conjunction with very large arrays. Very low dark current and multispectral capability are possible because of hardware compatibility with the cryogenic environment of high performance detector arrays. The aforementioned hardware development effort is aimed at systems which would integrate image acquisition and image processing.

  18. Spatiotemporal features for asynchronous event-based data

    PubMed Central

    Lagorce, Xavier; Ieng, Sio-Hoi; Clady, Xavier; Pfeiffer, Michael; Benosman, Ryad B.

    2015-01-01

    Bio-inspired asynchronous event-based vision sensors are currently introducing a paradigm shift in visual information processing. These new sensors rely on a stimulus-driven principle of light acquisition similar to biological retinas. They are event-driven and fully asynchronous, thereby reducing redundancy and encoding exact times of input signal changes, leading to a very precise temporal resolution. Approaches for higher-level computer vision often rely on the reliable detection of features in visual frames, but similar definitions of features for the novel dynamic and event-based visual input representation of silicon retinas have so far been lacking. This article addresses the problem of learning and recognizing features for event-based vision sensors, which capture properties of truly spatiotemporal volumes of sparse visual event information. A novel computational architecture for learning and encoding spatiotemporal features is introduced based on a set of predictive recurrent reservoir networks, competing via winner-take-all selection. Features are learned in an unsupervised manner from real-world input recorded with event-based vision sensors. It is shown that the networks in the architecture learn distinct and task-specific dynamic visual features, and can predict their trajectories over time. PMID:25759637

  19. Formation of the wide asynchronous binary asteroid population

    SciTech Connect

    Jacobson, Seth A.; Scheeres, Daniel J.; McMahon, Jay

    2014-01-01

    We propose and analyze a new mechanism for the formation of the wide asynchronous binary population. These binary asteroids have wide semimajor axes relative to most near-Earth and main belt asteroid systems. Confirmed members have rapidly rotating primaries and satellites that are not tidally locked. Previously suggested formation mechanisms from impact ejecta, from planetary flybys, and directly from rotational fission events cannot satisfy all of the observations. The newly hypothesized mechanism works as follows: (1) these systems are formed from rotational fission, (2) their satellites are tidally locked, (3) their orbits are expanded by the binary Yarkovsky-O'Keefe-Radzievskii-Paddack (BYORP) effect, (4) their satellites desynchronize as a result of the adiabatic invariance between the libration of the secondary and the mutual orbit, and (5) the secondary avoids resynchronization because of the YORP effect. This seemingly complex chain of events is a natural pathway for binaries with satellites that have particular shapes, which define the BYORP effect torque that acts on the system. After detailing the theory, we analyze each of the wide asynchronous binary members and candidates to assess their most likely formation mechanism. Finally, we suggest possible future observations to check and constrain our hypothesis.

  20. Bowhead whale localization using asynchronous hydrophones in the Chukchi Sea.

    PubMed

    Warner, Graham A; Dosso, Stan E; Hannay, David E; Dettmer, Jan

    2016-07-01

    This paper estimates bowhead whale locations and uncertainties using non-linear Bayesian inversion of their modally-dispersed calls recorded on asynchronous recorders in the Chukchi Sea, Alaska. Bowhead calls were recorded on a cluster of 7 asynchronous ocean-bottom hydrophones that were separated by 0.5-9.2 km. A warping time-frequency analysis is used to extract relative mode arrival times as a function of frequency for nine frequency-modulated whale calls that dispersed in the shallow water environment. Each call was recorded on multiple hydrophones and the mode arrival times are inverted for: the whale location in the horizontal plane, source instantaneous frequency (IF), water sound-speed profile, seabed geoacoustic parameters, relative recorder clock drifts, and residual error standard deviations, all with estimated uncertainties. A simulation study shows that accurate prior environmental knowledge is not required for accurate localization as long as the inversion treats the environment as unknown. Joint inversion of multiple recorded calls is shown to substantially reduce uncertainties in location, source IF, and relative clock drift. Whale location uncertainties are estimated to be 30-160 m and relative clock drift uncertainties are 3-26 ms. PMID:27475129

  1. Asynchronously sampled blind source separation for coherent optical links

    NASA Astrophysics Data System (ADS)

    Detwiler, Thomas F.; Searcy, Steven M.; Stark, Andrew J.; Ralph, Stephen E.; Basch, Bert E.

    2011-01-01

    Polarization multiplexing is an integral technique for generating spectrally efficient 100 Gb/s and higher optical links. Post coherent detection DSP-based polarization demultiplexing of QPSK links is commonly performed after timing recovery. We propose and demonstrate a method of asynchronous blind source separation using the constant modulus algorithm (CMA) on the asynchronously sampled signal to initially separate energy from arbitrarily aligned polarization states. This method lends well to implementation as it allows for an open-loop sampling frequency for analog-to-digital conversion at less than twice the symbol rate. We show that the performance of subsequent receiver functions is enhanced by the initial pol demux operation. CMA singularity behavior is avoided through tap settling constraints. The method is applicable to QPSK transmissions and many other modulation formats as well, including general QAM signals, offset-QPSK, and CPM, or a combination thereof. We present the architecture and its performance under several different formats and link conditions. Comparisons of complexity and performance are drawn between the proposed architecture and conventional receivers.

  2. Experience with synchronous and asynchronous digital control systems. [for flight

    NASA Technical Reports Server (NTRS)

    Regenie, Victoria A.; Chacon, Claude V.; Lock, Wilton P.

    1986-01-01

    Flight control systems have undergone a revolution since the days of simple mechanical linkages; presently the most advanced systems are full-authority, full-time digital systems controlling unstable aircraft. With the use of advanced control systems, the aerodynamic design can incorporate features that allow greater performance and fuel savings, as can be seen on the new Airbus design and advanced tactical fighter concepts. These advanced aircraft will be and are relying on the flight control system to provide the stability and handling qualities required for safe flight and to allow the pilot to control the aircraft. Various design philosophies have been proposed and followed to investigate system architectures for these advanced flight control systems. One major area of discussion is whether a multichannel digital control system should be synchronous or asynchronous. This paper addressed the flight experience at the Dryden Flight Research Facility of NASA's Ames Research Center with both synchronous and asynchronous digital flight control systems. Four different flight control systems are evaluated against criteria such as software reliability, cost increases, and schedule delays.

  3. Asynchronous Transfer Mode Quality-of-Service Testing

    NASA Technical Reports Server (NTRS)

    Ivancic, William D.

    1998-01-01

    In support of satellite-ATM interoperability, researchers at the NASA Lewis Research Center performed asynchronous transfer mode (ATM) quality-of-service experiments using MPEG-2 (ATM application layer 5, AAL5) over ATM over an emulated satellite link. The purpose of these experiments was to determine the free-space link quality necessary to use the ATM protocol to transmit high-quality multimedia information. The experimental results have been submitted to various International Telecommunications Union (ITU) study groups in order to improve and modify current standards and recommendations for the telecommunications industry. Quality-of-service parameters for Class I, stringent class requirements for ITU-T I.356 are currently being debated. The experimental results presented will help to establish these quality-of-service thresholds. This material will also be useful in the development of the ITU-R WP-4B's Draft Preliminary New Recommendation on the Transmission of Asynchronous Transfer Mode Traffic via Satellite (Rec. S.atm).

  4. Emergence of Asynchronous Local Clocks in Excitable Media

    PubMed Central

    Gerum, Richard Carl; Fabry, Ben; Metzner, Claus

    2015-01-01

    Excitable media such as the myocardium or the brain consist of arrays of coupled excitable elements, in which the local excitation of a single element can propagate to its neighbors in the form of a non-linear autowave. Since each element has to pass through a refractory period immediately after excitation, the frequency of autowaves is self-limiting. In this work, we consider the case where each element is spontaneously excited at a fixed average rate and thereby initiates a new autowave. Although these spontaneous self-excitation events are modelled as independent Poisson point processes with exponentially distributed waiting times, the travelling autowaves lead collectively to a non-exponential, unimodal waiting time distribution for the individual elements. With increasing system size, a global ‘clock’ period T emerges as the most probable waiting time for each element, which fluctuates around T with an increasingly small but non-zero variance. This apparent synchronization between asynchronous, temporally uncorrelated point processes differs from synchronization effects between perfect oscillators interacting in a phase-aligning manner. Finally, we demonstrate that asynchronous local clocks also emerge in non-homogeneous systems in which the rates of self-excitation are different for all individuals, suggesting that this novel mechanism can occur in a wide range of excitable media. PMID:26559528

  5. Asynchronous algorithms for heat conductors in composite media

    SciTech Connect

    El-Tarazi, M.N.; Anwar, M.N. )

    1989-01-01

    In this paper the authors treat the problem of two dimensional steady-state heat conduction in a medium composed of two homogeneous and isotropic, but physically dissimilar materials. In situations where the boundary conditions are not simple elementary functions that exact analytical solution of such problems is impossible even for regions having a simple geometry. Thus a numerical technique is needed and the authors propose in this paper to discretize the considered heat conduction problem by the method of lines to obtain a system of second order differential equations with multipoint boundary conditions. This differential system is converted, using invariant imbedding for each one dimensional problem, into a fixed point problem for which several iterative algorithms can be used. If the asynchronous parallel algorithms are used, we obtain an approach which combines both simplicity (referring to the classical invariant imbedding associated with the by lines approximation) and efficiency (referring to the asynchronous parallel algorithms). In Section 2, The authors give the mathematical formulation of the problem considered in this paper together with the appropriate boundary and auxiliary conditions.

  6. Experience with synchronous and asynchronous digital control systems

    NASA Technical Reports Server (NTRS)

    Regenie, V. A.; Chacon, C. V.; Lock, W. P.

    1986-01-01

    Flight control systems have undergone a revolution since the days of simple mechanical linkages; presently the most advanced systems are full-authority, full-time digital systems controlling unstable aircraft. With the use of advanced control systems, the aerodynamic design can incorporate features that allow greater performance and fuel savings, as can be seen on the new Airbus design and advanced tactical fighter concepts. These advanced aircraft will be and are relying on the flight control system to provide the stability and handling qualities required for safe flight and to allow the pilot to control the aircraft. Various design philosophies have been proposed and followed to investigate system architectures for these advanced flight control systems. One major area of discussion is whether a multichannel digital control system should be synchronous or asynchronous. This paper addressed the flight experience at the Dryden Flight Research Facility of NASA's Ames Research Center with both synchronous and asynchronous digital flight control systems. Four different flight control systems are evaluated against criteria such as software reliability, cost increases, and schedule delays.

  7. A Parallel Particle Swarm Optimization Algorithm Accelerated by Asynchronous Evaluations

    NASA Technical Reports Server (NTRS)

    Venter, Gerhard; Sobieszczanski-Sobieski, Jaroslaw

    2005-01-01

    A parallel Particle Swarm Optimization (PSO) algorithm is presented. Particle swarm optimization is a fairly recent addition to the family of non-gradient based, probabilistic search algorithms that is based on a simplified social model and is closely tied to swarming theory. Although PSO algorithms present several attractive properties to the designer, they are plagued by high computational cost as measured by elapsed time. One approach to reduce the elapsed time is to make use of coarse-grained parallelization to evaluate the design points. Previous parallel PSO algorithms were mostly implemented in a synchronous manner, where all design points within a design iteration are evaluated before the next iteration is started. This approach leads to poor parallel speedup in cases where a heterogeneous parallel environment is used and/or where the analysis time depends on the design point being analyzed. This paper introduces an asynchronous parallel PSO algorithm that greatly improves the parallel e ciency. The asynchronous algorithm is benchmarked on a cluster assembled of Apple Macintosh G5 desktop computers, using the multi-disciplinary optimization of a typical transport aircraft wing as an example.

  8. Asynchronous replica exchange software for grid and heterogeneous computing

    NASA Astrophysics Data System (ADS)

    Gallicchio, Emilio; Xia, Junchao; Flynn, William F.; Zhang, Baofeng; Samlalsingh, Sade; Mentes, Ahmet; Levy, Ronald M.

    2015-11-01

    Parallel replica exchange sampling is an extended ensemble technique often used to accelerate the exploration of the conformational ensemble of atomistic molecular simulations of chemical systems. Inter-process communication and coordination requirements have historically discouraged the deployment of replica exchange on distributed and heterogeneous resources. Here we describe the architecture of a software (named ASyncRE) for performing asynchronous replica exchange molecular simulations on volunteered computing grids and heterogeneous high performance clusters. The asynchronous replica exchange algorithm on which the software is based avoids centralized synchronization steps and the need for direct communication between remote processes. It allows molecular dynamics threads to progress at different rates and enables parameter exchanges among arbitrary sets of replicas independently from other replicas. ASyncRE is written in Python following a modular design conducive to extensions to various replica exchange schemes and molecular dynamics engines. Applications of the software for the modeling of association equilibria of supramolecular and macromolecular complexes on BOINC campus computational grids and on the CPU/MIC heterogeneous hardware of the XSEDE Stampede supercomputer are illustrated. They show the ability of ASyncRE to utilize large grids of desktop computers running the Windows, MacOS, and/or Linux operating systems as well as collections of high performance heterogeneous hardware devices.

  9. Asynchronous compressed beamformer for portable diagnostic ultrasound systems.

    PubMed

    Zhou, Jun; Hoyos, Sebastian; Sadler, Brian

    2014-11-01

    State-of-the-art portable ultrasound imaging systems employ a small transducer array and a low carrier frequency to fit stringent constraints on power and form factor, and this tends to compromise the ultrasound imaging quality. In this paper, we present a low-complexity low-power asynchronous compressed beamformer (ACB) for portable diagnostic ultrasound. The proposed ACB integrates asynchronous sampling and compressive sensing (CS), and is capable of reducing data conversion power and handling a large data volume at the mixed-signal interface. A high-rate continuoustime ternary encoding (CT-TE) scheme eliminates the need for interpolation filters and coordinate rotation digital computer (CORDIC) units typically used in a conventional architecture. A split-projection least squares (SPLS) signal reconstruction algorithm is applied that replaces high-cost nonlinear signal recovery with a series of low-complexity and independent linear problems. Experiments with measured ultrasound data demonstrate the proposed ACB architecture, and the SPLS reconstruction algorithm achieves 9-fold data compression compared with Nyquist sampling. PMID:25389158

  10. Asynchronous Detection of Trials Onset from Raw EEG Signals.

    PubMed

    Lopez-Gordo, M A; Grima Murcia, M D; Padilla, Pablo; Pelayo, F; Fernandez, E

    2016-11-01

    Clinical processing of event-related potentials (ERPs) requires a precise synchrony between the stimulation and the acquisition units that are guaranteed by means of a physical link between them. This precise synchrony is needed since temporal misalignments during trial averaging can lead to high deviations of peak times, thus causing error in diagnosis or inefficiency in classification in brain-computer interfaces (BCIs). Out of the laboratory, mobile EEG systems and BCI headsets are not provided with the physical link, thus being inadequate for acquisition of ERPs. In this study, we propose a method for the asynchronous detection of trials onset from raw EEG without physical links. We validate it with a BCI application based on the dichotic listening task. The user goal was to attend the cued auditory message and to report three keywords contained in it while ignoring the other message. The BCI goal was to detect the attended message from the analysis of auditory ERPs. The rate of successful onset detection in both synchronous (using the real onset) and asynchronous (blind detection of trial onset from raw EEG) was 73% with a synchronization error of less than 1[Formula: see text]ms. The level of synchronization provided by this proposal would allow home-based acquisition of ERPs with low cost BCI headsets and any media player unit without physical links between them. PMID:27377663

  11. An Examination of Asynchronous Communication Experiences and Perspectives of Students in an Online Course: A Case Study.

    ERIC Educational Resources Information Center

    Vonderwell, Selma

    2003-01-01

    Explores asynchronous communication perspectives and experiences of undergraduate students in an online course. Interviews with 22 preservice teachers, student-to-instructor email transcripts, asynchronous discussion transcripts, and two independent reviewers' reviews of the asynchronous discussions were used to collect and analyze the data.…

  12. Asynchronous Multilateral Control for Simplified Walking Haptic System

    NASA Astrophysics Data System (ADS)

    Okura, Takeshi; Katsura, Seiichiro

    Recently, there has been extensive development in remote communication technologies such as telephone, radio, and television. Haptic information is studied as the third type of multimedia information. Real-world haptics is getting attention not only for the feedback of real-world haptic information in teleoperation but also as the key technology for future human support. Most of the objects of study in haptics are stationary. Therefore, the moving range of such haptic systems is limited. This paper proposes a novel haptic device that receives force feedback of walking motion from a remote environment and manipulates remote system. Thus, its moving range is unrestricted. To drive this haptic system, the paper also proposes asynchronous multilateral control. By using this control, an operator can operate the remote system without changing his/her position. The proposed haptic system is expected to act as an effective interface for virtual reality applications and teleoperation.

  13. An Asynchronous Cellular Automata-Based Adaptive Illumination Facility

    NASA Astrophysics Data System (ADS)

    Bandini, Stefania; Bonomi, Andrea; Vizzari, Giuseppe; Acconci, Vito

    The term Ambient Intelligence refers to electronic environments that are sensitive and responsive to the presence of people; in the described scenario the environment itself is endowed with a set of sensors (to perceive humans or other physical entities such as dogs, bicycles, etc.), interacting with a set of actuators (lights) that choose their actions (i.e. state of illumination) in an attempt improve the overall experience of these users. The model for the interaction and action of sensors and actuators is an asynchronous Cellular Automata (CA) with memory, supporting a self-organization of the system as a response to the presence and movements of people inside it. The paper will introduce the model, as well as an ad hoc user interface for the specification of the relevant parameters of the CA transition rule that determines the overall system behaviour.

  14. Vector control structure of an asynchronous motor at maximum torque

    NASA Astrophysics Data System (ADS)

    Chioncel, C. P.; Tirian, G. O.; Gillich, N.; Raduca, E.

    2016-02-01

    Vector control methods offer the possibility to gain high performance, being widely used. Certain applications require an optimum control in limit operating conditions, as, at maximum torque, that is not always satisfied. The paper presents how the voltage and the frequency for an asynchronous machine (ASM) operating at variable speed are determinate, with an accent on the method that keeps the rotor flux constant. The simulation analyses consider three load types: variable torque and speed, variable torque and constant speed, constant torque and variable speed. The final values of frequency and voltage are obtained through the proposed control schemes with one controller using the simulation language based on the Maple module. The dynamic analysis of the system is done for the case with P and PI controller and allows conclusions on the proposed method, which can have different applications, as the ASM in wind turbines.

  15. Minor Planet 1016 Anitra: A Likely Asynchronous Binary

    NASA Astrophysics Data System (ADS)

    Pilcher, Frederick; Benishek, Vladimir; Jacobsen, Jens; Kristensen, Leif Hugo; Lang, Kim; Larsen, Frank R.; Odden, Caroline; Pravec, Petr

    2016-07-01

    Analysis of photometry data for 1016 Anitra using a single period analysis shows a lightcurve with a period of 5.9296 hours and amplitude of 0.30 mag, with short period fluctuations. A simultaneous two-period analysis of the data found two smooth bimodal lightcurves with periods and amplitudes of P1 = 5.92951 h, A1 = 0.30 mag and P2 = 2.609143 h, A2 = 0.10 mag. These results are interpreted as the rotation of the two components of an asynchronous binary system. Four additional attenuations were observed that may have been caused by satellite mutual events, but those data were insufficient to yield an orbital period.

  16. Asynchronous transfer mode link performance over ground networks

    NASA Technical Reports Server (NTRS)

    Chow, E. T.; Markley, R. W.

    1993-01-01

    The results of an experiment to determine the feasibility of using asynchronous transfer mode (ATM) technology to support advanced spacecraft missions that require high-rate ground communications and, in particular, full-motion video are reported. Potential nodes in such a ground network include Deep Space Network (DSN) antenna stations, the Jet Propulsion Laboratory, and a set of national and international end users. The experiment simulated a lunar microrover, lunar lander, the DSN ground communications system, and distributed science users. The users were equipped with video-capable workstations. A key feature was an optical fiber link between two high-performance workstations equipped with ATM interfaces. Video was also transmitted through JPL's institutional network to a user 8 km from the experiment. Variations in video depending on the networks and computers were observed, the results are reported.

  17. Asynchronous Motor Nonlinear Control with Inverter Output LC Filter

    NASA Astrophysics Data System (ADS)

    Guzinski, J.; Abu-Rub, H.

    2009-03-01

    In many adjustable electric drives the LC filter is used to smooth the voltages and currents supplying AC motors. The LC filter makes it difficult to implement precise control of these motors especially in the case of speed sensorless solution. Because measuring motor currents and capacitor voltages is not practical, a preferable solution is to measure only filter input current. A good approach is to use observers to restore unmeasured state variables. Additionally, it is necessary to modify the used control in case of the drive with LC filter. In this paper the proposed system with nonlinear control method of the asynchronous motor, and the speed and flux observer structure were been adapted regarding the LC filter. In the presented drive only sensors previously used in the system without LC filter were used. 1.5 kW drive was verified in by simulation and experiments.

  18. Asynchronous extinction of late Quaternary sloths on continents and islands

    PubMed Central

    Steadman, David W.; Martin, Paul S.; MacPhee, Ross D. E.; Jull, A. J. T.; McDonald, H. Gregory; Woods, Charles A.; Iturralde-Vinent, Manuel; Hodgins, Gregory W. L.

    2005-01-01

    Whatever the cause, it is extraordinary that dozens of genera of large mammals became extinct during the late Quaternary throughout the Western Hemisphere, including 90% of the genera of the xenarthran suborder Phyllophaga (sloths). Radiocarbon dates directly on dung, bones, or other tissue of extinct sloths place their “last appearance” datum at ≈11,000 radiocarbon years before present (yr BP) or slightly less in North America, ≈10,500 yr BP in South America, and ≈4,400 yr BP on West Indian islands. This asynchronous situation is not compatible with glacial–interglacial climate change forcing these extinctions, especially given the great elevational, latitudinal, and longitudinal variation of the sloth-bearing continental sites. Instead, the chronology of last appearance of extinct sloths, whether on continents or islands, more closely tracks the first arrival of people. PMID:16085711

  19. Security technologies and protocols for Asynchronous Transfer Mode networks

    SciTech Connect

    Tarman, T.D.

    1996-06-01

    Asynchronous Transfer Mode (ATM) is a new data communications technology that promises to integrate voice, video, and data traffic into a common network infrastructure. In order to fully utilize ATM`s ability to transfer real-time data at high rates, applications will start to access the ATM layer directly. As a result of this trend, security mechanisms at the ATM layer will be required. A number of research programs are currently in progress which seek to better understand the unique issues associated with ATM security. This paper describes some of these issues, and the approaches taken by various organizations in the design of ATM layer security mechanisms. Efforts within the ATM Forum to address the user communities need for ATM security are also described.

  20. Reliable asynchronous image transfer protocol in wireless multimedia sensor networks.

    PubMed

    Lee, Joa-Hyoung; Jung, In-Bum

    2010-01-01

    In the paper, we propose a reliable asynchronous image transfer protocol, RAIT. RAIT applies a double sliding window method to node-to-node transfer, with one sliding window for the receiving queue, which is used to prevent packet loss caused by communication failure between nodes, and another sliding window for the sending queue, which prevents packet loss caused by network congestion. The routing node prevents packet loss between nodes by preemptive scheduling of multiple packets for a given image. RAIT implements a double sliding window method by means of a cross-layer design between the RAIT layer, routing layer, and queue layer. We demonstrate that RAIT guarantees a higher reliability of image transmission compared to the existing protocols. PMID:22294883

  1. Immunoglobulins from amyotrophic lateral sclerosis patients enhance spontaneous transmitter release from motor-nerve terminals.

    PubMed Central

    Uchitel, O D; Appel, S H; Crawford, F; Sczcupak, L

    1988-01-01

    Amyotrophic lateral sclerosis (ALS) is an incapacitating neuromuscular disease of unknown etiology. Although laboratory evidence is lacking, circumstantial evidence supports the importance of immune factors in the pathogenesis of ALS. In the present study immunoglobulins from 4 of 8 ALS patients induced a significant increase in spontaneous quantal transmitter release as monitored by miniature end-plate potential (MEPP) frequency in mouse phrenic nerve-diaphragm preparations at 23 degrees C, whereas immunoglobulins from normal individuals and from patients with other neuromuscular diseases had no effect. At 32 degrees C neither normal nor disease control immunoglobulins influenced MEPP frequency, but 8 of 11 ALS immunoglobulin samples produced a significant increase in spontaneous quantal transmitter release. The enhancing effect could be prevented by 10 mM Mg2+. No effects were noted on MEPP amplitude and muscle resting potential. These findings suggest that the presynaptic terminal of the motor neuron may be an early target and that immunological factors may play an important role in the disease process. PMID:2902629

  2. Asynchronous learning: student utilization out of sync with their preference

    PubMed Central

    Lew, Edward K.; Nordquist, Erik K.

    2016-01-01

    Background Asynchronous learning is gaining popularity. Data are limited regarding this learning method in medical students rotating in emergency medicine (EM). In EM, faculty time is limited to give in-person lectures. The authors sought to create an online curriculum that students could utilize as an additional learning modality. Objective The goal was to evaluate effectiveness, participation, and preference for this mode of learning. Methods We developed five online, narrated PowerPoint presentations. After orientation, access to the online curriculum was provided to the students, which they could review at their leisure. Results One hundred and seven fourth-year medical students participated. They reported the curriculum to be of high quality. Pretest scores were similar for those that viewed all lectures – compliant group (CG) (9.5 [CI 4.8–14.1]) and those that did not view any – non-compliant group (NCG) (9.6 [CI 5.9–13.4]). There was no statistical significant difference in posttest scores between the groups although there was improvement overall: CG 14.6 (CI 6.9–22.1); NCG 11.4 (CI 5.7–17.1). A majority (69.2%) favored inclusion of asynchronous learning, but less than a quarter (22.4%) reported viewing all five modules and more than a third (36.4%) viewed none. Conclusion Despite student-expressed preference for an online curriculum, they used the online resource less than expected. This should give pause to educators looking to convert core EM topics to an online format. However, when high-quality online lectures are utilized as a learning tool, this study demonstrates that they had neither a positive nor a negative impact on test scores. PMID:27280385

  3. Modeling and Analysis of Asynchronous Systems Using SAL and Hybrid SAL

    NASA Technical Reports Server (NTRS)

    Tiwari, Ashish; Dutertre, Bruno

    2013-01-01

    We present formal models and results of formal analysis of two different asynchronous systems. We first examine a mid-value select module that merges the signals coming from three different sensors that are each asynchronously sampling the same input signal. We then consider the phase locking protocol proposed by Daly, Hopkins, and McKenna. This protocol is designed to keep a set of non-faulty (asynchronous) clocks phase locked even in the presence of Byzantine-faulty clocks on the network. All models and verifications have been developed using the SAL model checking tools and the Hybrid SAL abstractor.

  4. Modeling and measurement of vesicle pools at the cone ribbon synapse: Changes in release probability are solely responsible for voltage-dependent changes in release.

    PubMed

    Thoreson, Wallace B; Van Hook, Matthew J; Parmelee, Caitlyn; Curto, Carina

    2016-01-01

    Postsynaptic responses are a product of quantal amplitude (Q), size of the releasable vesicle pool (N), and release probability (P). Voltage-dependent changes in presynaptic Ca(2+) entry alter postsynaptic responses primarily by changing P but have also been shown to influence N. With simultaneous whole cell recordings from cone photoreceptors and horizontal cells in tiger salamander retinal slices, we measured N and P at cone ribbon synapses by using a train of depolarizing pulses to stimulate release and deplete the pool. We developed an analytical model that calculates the total pool size contributing to release under different stimulus conditions by taking into account the prior history of release and empirically determined properties of replenishment. The model provided a formula that calculates vesicle pool size from measurements of the initial postsynaptic response and limiting rate of release evoked by a train of pulses, the fraction of release sites available for replenishment, and the time constant for replenishment. Results of the model showed that weak and strong depolarizing stimuli evoked release with differing probabilities but the same size vesicle pool. Enhancing intraterminal Ca(2+) spread by lowering Ca(2+) buffering or applying BayK8644 did not increase PSCs evoked with strong test steps, showing there is a fixed upper limit to pool size. Together, these results suggest that light-evoked changes in cone membrane potential alter synaptic release solely by changing release probability. PMID:26541100

  5. Distributed Consensus of Stochastic Delayed Multi-agent Systems Under Asynchronous Switching.

    PubMed

    Wu, Xiaotai; Tang, Yang; Cao, Jinde; Zhang, Wenbing

    2016-08-01

    In this paper, the distributed exponential consensus of stochastic delayed multi-agent systems with nonlinear dynamics is investigated under asynchronous switching. The asynchronous switching considered here is to account for the time of identifying the active modes of multi-agent systems. After receipt of confirmation of mode's switching, the matched controller can be applied, which means that the switching time of the matched controller in each node usually lags behind that of system switching. In order to handle the coexistence of switched signals and stochastic disturbances, a comparison principle of stochastic switched delayed systems is first proved. By means of this extended comparison principle, several easy to verified conditions for the existence of an asynchronously switched distributed controller are derived such that stochastic delayed multi-agent systems with asynchronous switching and nonlinear dynamics can achieve global exponential consensus. Two examples are given to illustrate the effectiveness of the proposed method. PMID:26292354

  6. Mathematical Description of an Asynchronous Motor with the Indirect Control of the Output Mechanical Variables

    NASA Astrophysics Data System (ADS)

    Glazachev, A. V.; Dementyev, Yu. N.; Negodin, K. N.; Umursakova, A. D.

    2016-02-01

    The article gives the mathematical description of an asynchronous motor with the indirect control of the output mechanical variables of an asynchronous motor in the electric drive. To determine the electromagnetic torque and angular velocity of the asynchronous motor in the electric drive the mathematical description is used in which the values are determined by the readings of the motor and easily measured values by means of known in practice devices. The proposed in the article the mathematical description for the indirect measuring the electromagnetic torque and angular velocity of the asynchronous motor in the electric drive does not contain the integral components that introduce the great error into the value of the controlled electromagnetic torque and angular velocity.

  7. Strict optical orthogonal codes for purely asynchronous code-division multiple-access applications.

    PubMed

    Zhang, J G

    1996-12-10

    Strict optical orthogonal codes are presented for purely asynchronous optical code-division multiple-access (CDMA) applications. The proposed code can strictly guarantee the peaks of its cross-correlation functions and the sidelobes of any of its autocorrelation functions to have a value of 1 in purely asynchronous data communications. The basic theory of the proposed codes is given. An experiment on optical CDMA systems is also demonstrated to verify the characteristics of the proposed code. PMID:21151299

  8. Multitude of ion channels in the regulation of transmitter release.

    PubMed Central

    Rahamimoff, R; Butkevich, A; Duridanova, D; Ahdut, R; Harari, E; Kachalsky, S G

    1999-01-01

    The presynaptic nerve terminal is of key importance in communication in the nervous system. Its primary role is to release transmitter quanta on the arrival of an appropriate stimulus. The structural basis of these transmitter quanta are the synaptic vesicles that fuse with the surface membrane of the nerve terminal, to release their content of neurotransmitter molecules and other vesicular components. We subdivide the control of quantal release into two major classes: the processes that take place before the fusion of the synaptic vesicle with the surface membrane (the pre-fusion control) and the processes that occur after the fusion of the vesicle (the post-fusion control). The pre-fusion control is the main determinant of transmitter release. It is achieved by a wide variety of cellular components, among them the ion channels. There are reports of several hundred different ion channel molecules at the surface membrane of the nerve terminal, that for convenience can be grouped into eight major categories. They are the voltage-dependent calcium channels, the potassium channels, the calcium-gated potassium channels, the sodium channels, the chloride channels, the non-selective channels, the ligand gated channels and the stretch-activated channels. There are several categories of intracellular channels in the mitochondria, endoplasmic reticulum and the synaptic vesicles. We speculate that the vesicle channels may be of an importance in the post-fusion control of transmitter release. PMID:10212476

  9. Comparing face-to-face, synchronous, and asynchronous learning: postgraduate dental resident preferences.

    PubMed

    Kunin, Marc; Julliard, Kell N; Rodriguez, Tobias E

    2014-06-01

    The Department of Dental Medicine of Lutheran Medical Center has developed an asynchronous online curriculum consisting of prerecorded PowerPoint presentations with audio explanations. The focus of this study was to evaluate if the new asynchronous format satisfied the educational needs of the residents compared to traditional lecture (face-to-face) and synchronous (distance learning) formats. Lectures were delivered to 219 dental residents employing face-to-face and synchronous formats, as well as the new asynchronous format; 169 (77 percent) participated in the study. Outcomes were assessed with pretests, posttests, and individual lecture surveys. Results found the residents preferred face-to-face and asynchronous formats to the synchronous format in terms of effectiveness and clarity of presentations. This preference was directly related to the residents' perception of how well the technology worked in each format. The residents also rated the quality of student-instructor and student-student interactions in the synchronous and asynchronous formats significantly higher after taking the lecture series than they did before taking it. However, they rated the face-to-face format as significantly more conducive to student-instructor and student-student interaction. While the study found technology had a major impact on the efficacy of this curricular model, the results suggest that the asynchronous format can be an effective way to teach a postgraduate course. PMID:24882771

  10. Simulating Quantitative Cellular Responses Using Asynchronous Threshold Boolean Network Ensembles

    PubMed Central

    2011-01-01

    Background With increasing knowledge about the potential mechanisms underlying cellular functions, it is becoming feasible to predict the response of biological systems to genetic and environmental perturbations. Due to the lack of homogeneity in living tissues it is difficult to estimate the physiological effect of chemicals, including potential toxicity. Here we investigate a biologically motivated model for estimating tissue level responses by aggregating the behavior of a cell population. We assume that the molecular state of individual cells is independently governed by discrete non-deterministic signaling mechanisms. This results in noisy but highly reproducible aggregate level responses that are consistent with experimental data. Results We developed an asynchronous threshold Boolean network simulation algorithm to model signal transduction in a single cell, and then used an ensemble of these models to estimate the aggregate response across a cell population. Using published data, we derived a putative crosstalk network involving growth factors and cytokines - i.e., Epidermal Growth Factor, Insulin, Insulin like Growth Factor Type 1, and Tumor Necrosis Factor α - to describe early signaling events in cell proliferation signal transduction. Reproducibility of the modeling technique across ensembles of Boolean networks representing cell populations is investigated. Furthermore, we compare our simulation results to experimental observations of hepatocytes reported in the literature. Conclusion A systematic analysis of the results following differential stimulation of this model by growth factors and cytokines suggests that: (a) using Boolean network ensembles with asynchronous updating provides biologically plausible noisy individual cellular responses with reproducible mean behavior for large cell populations, and (b) with sufficient data our model can estimate the response to different concentrations of extracellular ligands. Our results suggest that this