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Sample records for atf damping ring

  1. High resolution upgrade of the ATF damping ring BPM system

    SciTech Connect

    Terunuma, N.; Urakawa, J.; Frisch, J.; May, J.; McCormick, D.; Nelson, J.; Seryi, A.; Smith, T.; Woodley, M.; Briegel, C.; Dysert, R.; /Fermilab

    2008-05-01

    A beam position monitor (BPM) upgrade at the KEK Accelerator Test Facility (ATF) damping ring has been accomplished in its first stage, carried out by a KEK/FNAL/SLAC collaboration under the umbrella of the global ILC R&D effort. The upgrade consists of a high resolution, high reproducibility read-out system, based on analog and digital downconversion techniques, digital signal processing, and also tests a new automatic gain error correction schema. The technical concept and realization, as well as preliminary results of beam studies are presented.

  2. Status of the ATF Damping Ring BPM Upgrade Project

    SciTech Connect

    Briegel, C.; Eddy, N.; Haynes, B.; May, J.; McCormick, D.; Nelson, J.; Nicklaus, D.; Prieto, P.; Rechenmacher, R.; Smith, T.; Teranuma, N.; Urakawa, J.; Voy, D.; Wendt, M.; Woodley, M.; /SLAC

    2011-12-01

    A substantial upgrade of the beam position monitors (BPM) at the ATF (Accelerator Test Facility) damping ring is currently in progress. Implementing digital read-out signal processing techniques in line with an optimized, low-noise analog downconverter, a resolution well below 1 mum could be demonstrated at 20 (of 96) upgraded BPM stations. The narrowband, high resolution BPM mode permits investigation of all types of non-linearities, imperfections and other obstacles in the machine which may limit the very low target aimed vertical beam emittance of < 2 pm. The technical status of the project, first beam measurements and an outlook to it's finalization are presented.

  3. High Resolution BPM Upgrade for the ATF Damping Ring at KEK

    SciTech Connect

    Eddy, N.; Briegel, C.; Fellenz, B.; Gianfelice-Wendt, E.; Prieto, P.; Rechenmacher, R.; Semenov, A.; Voy, D.; Wendt, M.; Zhang, D.; Terunuma, N.; /KEK, Tsukuba

    2011-08-17

    A beam position monitor (BPM) upgrade at the KEK Accelerator Test Facility (ATF) damping ring has been accomplished, carried out by a KEK/FNAL/SLAC collaboration under the umbrella of the global ILC R&D effort. The upgrade consists of a high resolution, high reproducibility read-out system, based on analog and digital down-conversion techniques, digital signal processing, and also implements a new automatic gain error correction schema. The technical concept and realization as well as results of beam studies are presented. The next generation of linear colliders require ultra-low vertical emittance of <2 pm-rad. The damping ring at the KEK Accelerator Test Facility (ATF) is designed to demonstrate this mission critical goal. A high resolution beam position monitor (BPM) system for the damping ring is one of the key tools for realizing this goal. The BPM system needs to provide two distnict measurements. First, a very high resolution ({approx}100-200nm) closed-orbit measurement which is averaged over many turns and realized with narrowband filter techniques - 'narrowband mode'. This is needed to monitor and steer the beam along an optimum orbit and to facilitate beam-based alignment to minimize non-linear field effects. Second, is the ability to make turn by turn (TBT) measurements to support optics studies and corrections necessary to achieve the design performance. As the TBT measurement necessitates a wider bandwidth, it is often referred to as 'wideband mode'. The BPM upgrade was initiated as a KEK/SLAC/FNAL collaboration in the frame of the Global Design Initiative of the International Linear Collider. The project was realized and completed using Japan-US funds with Fermilab as the core partner.

  4. Damping Wiggler Study at KEK-ATF

    SciTech Connect

    Naito, T.; Hayano, H.; Honda, Y.; Kubo, K.; Kuriki, M.; Kuroda, S.; Muto, T.; Terunuma, N.; Urakawa, J.; Sakai, H.; Nakamura, N.; Korostelev, M.; Zimmermann, F.; Ross, Marc; /SLAC

    2006-02-07

    The effects of damping wiggler magnets have been studied at KEK-ATF damping ring, which is a 1.3 GeV storage ring capable of producing ultra-low emittance electron beams. The fast beam damping is a significant issue for the damping ring. The tuning method with 4 sets of wiggler magnets was investigated for the ultra-low emittance beam. The effect on the beam quality, which is related to the transverse (x and y) and the longitudinal (z and {Delta}p/p), has been measured by the wire scanner, SR monitor, the laser wire, streak camera and the energy spread monitor. We report on the operational condition and the measurement results.

  5. Exotic damping ring lattices

    SciTech Connect

    Palmer, R.B.

    1987-05-01

    This paper looks at, and compares three types of damping ring lattices: conventional, wiggler lattice with finite ..cap alpha.., wiggler lattice with ..cap alpha.. = 0, and observes the attainable equilibrium emittances for the three cases assuming a constraint on the attainable longitudinal impedance of 0.2 ohms. The emittance obtained are roughly in the ratio 4:2:1 for these cases.

  6. New concepts for damping rings

    SciTech Connect

    Raimondi, P.; Wolski, A.

    2002-05-30

    The requirements for very low emittance and short damping time in the damping rings of future linear colliders, naturally lead to very small beta functions and dispersion in the ring arcs. This makes it difficult to make chromatic correction while maintaining good dynamics. We have therefore developed a lattice with very simple arcs (designed to give the best product of emittance and damping time), and with separate chromatic correction in a dedicated section. The chromatic correction is achieved using a series of non-interleaved sextupole pairs. The performance of such a solution is comparable to that of current damping ring designs, while there are a number of potential advantages.

  7. ATF2 Commissioning

    SciTech Connect

    Seryi, A.; Christian, G.; Parker, B.; Schulte, D.; Delahaye, J.-P.; Tomas, R.; Zimmermann, F.; Wolski, A.; Elsen, E.; Sanuki, T.; Gianfelice-Wendt, E.; Ross, M.; Wendt, M.; Takahashi, T.; Bai, S.; Gao, J.; Bolzon, B.; Geffroy, N.; Jeremie, A.; Apsimon, R.; Burrows, P.; /Oxford U., JAI /Kyoto U., Inst. Chem. Res. /Kyungpook Natl. U. /Orsay, LAL /Phang Accelerator Lab /Royal Holloway, U. of London /SLAC /Daresbury /University Coll. London /Manchester U. /Univ. of Tokyo U.

    2009-10-30

    ATF2 is a final-focus test beam line that aims to focus the low-emittance beam from the ATF damping ring to a beam size of about 37 nm, and at the same time to demonstrate nm beam stability, using numerous advanced beam diagnostics and feedback tools. The construction has been finished at the end of 2008 and the beam commissioning of ATF2 has started in December of 2008. ATF2 is constructed and commissioned by ATF international collaborations with strong US, Asian and European participation.

  8. Study for ILC Damping Ring at KEKB

    SciTech Connect

    Flanagan, J.W.; Fukuma, H.; Kanazawa, K.I.; Koiso, H.; Masuzawa, M.; Ohmi, Kazuhito; Ohnishi, Y.; Oide, Katsunobu; Suetsugu, Y.; Tobiyama, M.; Pivi, M.; /SLAC

    2011-11-04

    ILC damping ring consists of very low emittance electron and positron storage rings. It is necessary for ILC damping ring to study electron cloud effects in such low emittance positron ring. We propose a low emittance operation of KEKB to study the effects.

  9. The next linear collider damping ring lattices

    SciTech Connect

    Wolski, Andrzej; Corlett, John N.

    2001-06-20

    We report on the lattice design of the Next Linear Collider (NLC) damping rings. The damping rings are required to provide low emittance electron and positron bunch trains to the NLC linacs, at a rate of 120 Hz. We present an optical design, based on a theoretical minimum emittance (TME) lattice, to produce the required normalized extracted beam emittances gex = 3 mm-mrad and gey = 0.02 mm mrad. An assessment of dynamic aperture and non-linear effects is given. The positron pre-damping ring, required to reduce the emittance of the positron beam such that it may be accepted by a main damping ring, is also described.

  10. Bunch lengthening in the SLC damping ring

    SciTech Connect

    Rivkin, L.; Bane, K.; Chen, P.; Gabella, W.; Higo, T.; Hofmann, A.; Linebarger, W.; Kheifets, S.; Knight, T.; Morton, P.

    1988-05-01

    In this paper we present the results of measurements of bunch length and bunch shape as a function of current in the SLC e/sup /minus//damping ring. After extraction, the SLC bunch is compressed by means of an RF compressor and a subsequent high dispersion section. By inserting a video screen at a point of large dispersion and by using the correlation between bunch length and energy spread induced by the compressor, we have measured not only the bunch length but also the longitudinal charge distribution of the bunch in the damping ring as a function of beam intensity. At 3 /times/ 10/sup 10/ particles per bunch with a peak ring RF voltage of 800 KV, the FWHM of the bunch length in the ring doubles over the nominal value. To measure the energy spread of the bunch in the damping ring, the optics of the extraction lines was modified to produce a large dispersion but small horizontal ..beta.. function at the video screen. At 3 /times/ 10/sup 10/ particles per bunch, the relative energy spread in the rings is increased by about 30%. Finally, these data are compared with calculations of bunch lengthening in the SLC damping rings. 8 refs., 6 figs.,

  11. Status of the SLC damping rings

    SciTech Connect

    Hutton, A.M.; Davies-White, W.A.; Delahaye, J.P.; Fieguth, T.H.; Hofmann, A.; Jager, J.; Kloeppel, P.K.; Lee, M.J.; Linebarger, W.A.; Rivkin, L.

    1985-06-01

    Electron beams of full design energy 1.21 GeV and nearly full design intensity 4 x 10/sup 10/ particles/pulse (design 5 x 10/sup 10/) have been extracted from the Stanford Linac and successfully stored in the electron damping ring. Beams of less intensity have been extracted from the ring and reinjected into the Linac. The present intensity limits are not thought to be fundamental. The operating experience with the electron ring and the status of the construction of the positron ring will be discussed. 11 refs., 1 fig., 2 tabs.

  12. Intrabeam Scattering Studies for the ILC Damping Rings Using a NewMATLAB Code

    SciTech Connect

    Reichel, I.; Wolski, A.

    2006-06-21

    A new code to calculate the effects of intrabeam scattering (IBS) has been developed in MATLAB based on the approximation suggested by K. Bane. It interfaces with the Accelerator Toolbox but can also read in lattice functions from other codes. The code has been benchmarked against results from other codes for the ATF that use this approximation or do the calculation in a different way. The new code has been used to calculate the emittance growth due to intrabeam scattering for the lattices currently proposed for the ILC Damping Rings, as IBS is a concern, especially for the electron ring. A description of the code and its user interface, as well as results for the Damping Rings, will be presented.

  13. Dynamic apeerture in damping rings with realistic wigglers

    SciTech Connect

    Cai, Yunhai; /SLAC

    2005-05-04

    The International Linear Collider based on superconducting RF cavities requires the damping rings to have extremely small equilibrium emittance, huge circumference, fast damping time, and large acceptance. To achieve all of these requirements is a very challenging task. In this paper, we will present a systematic approach to designing the damping rings using simple cells and non-interlaced sextupoles. The designs of the damping rings with various circumferences and shapes, including dogbone, are presented. To model realistic wigglers, we have developed a new hybrid symplectic integrator for faster and accurate evaluation of dynamic aperture of the lattices.

  14. Damping ring designs for a TeV Linear Collider

    SciTech Connect

    Raubenheimer, T.O.; Rivkin, L.Z.; Ruth, R.D.

    1988-12-01

    In this paper we present a damping ring design for the TLC (TeV Linear Collider). The ring operates at 1.8 GeV. It has normalized emittances of elepsilon/sub x/ = 2.8 mrad and elepsilon/sub y/ = 25.4 nmrad. The damping times are /tau//sub x/ = 2.5 ms and /tau//sub y/ = 4.0 ms. To achieve these extremely low emittances and fast damping times, the ring contains 22 m of wigglers. 30 refs., 7 figs., 7 tabs.

  15. A damping ring design for the SLAC Next Linear Collider

    SciTech Connect

    Raubenheimer, T.O.; Byrd, J.; Corlett, J.

    1995-05-01

    In this paper, we describe the design of the main damping rings and the positron pre-damping ring for the SLAC Next Linear Collider, a future linear collider with a center-of-mass energy of 0.5 to 1.5 TeV. The rings will operate at an energy of 2 GeV with a maximum repetition rate of 180 Hz. The normalized extracted beam emittances are {gamma}{epsilon}{sub x} = 3 mm-mrad and {gamma}{epsilon}{sub y} = 0.03 mm-mrad. To provide the necessary damping, the rings must damp multiple trains of bunches. Thus, the beam current is large, roughly 1 A. We will present the optical layout, magnet designs, and RF systems, along with the dynamic aperture and required alignment tolerances; collective effects will be discussed in another paper.

  16. Design of the SLC damping ring to linac transport lines

    SciTech Connect

    Fieguth, T.H.; Murray, J.J.

    1983-07-01

    The first and second order optics for the damping ring to linac transport line are designed to preserve the damped transverse emittance while simultaneously compressing the bunch length of the beam to that length required for reinjection into the linac. This design, including provisions for future control of beam polarization, is described.

  17. Damping Ring R&D at CESR-TA

    SciTech Connect

    Rubin, David

    2015-01-23

    Accelerators that collide high energy beams of matter and anti-matter are essential tools for the investigation of the fundamental constituents of matter, and the search for new forms of matter and energy. A “Linear Collider” is a machine that would bring high energy and very compact bunches of electrons and positrons (anti-electrons) into head-on collision. Such a machine would produce (among many other things) the newly discovered Higgs particle, enabling a detailed study of its properties. Among the most critical and challenging components of a linear collider are the damping rings that produce the very compact and intense beams of electrons and positrons that are to be accelerated into collision. Hot dilute particle beams are injected into the damping rings, where they are compressed and cooled. The size of the positron beam must be reduced more than a thousand fold in the damping ring, and this compression must be accomplished in a fraction of a second. The cold compact beams are then extracted from the damping ring and accelerated into collision at high energy. The proposed International Linear Collider (ILC), would require damping rings that routinely produce such cold, compact and intense beams. The goal of the Cornell study was a credible design for the damping rings for the ILC. Among the technical challenges of the damping rings; the development of instrumentation that can measure the properties of the very small beams in a very narrow window of time, and mitigation of the forces that can destabilize the beams and prevent adequate cooling, or worse lead to beam loss. One of the most pernicious destabilizing forces is due to the formation of clouds of electrons in the beam pipe. The electron cloud effect is a phenomenon in particle accelerators in which a high density of low energy electrons, build up inside the vacuum chamber. At the outset of the study, it was anticipated that electron cloud effects would limit the intensity of the positron ring

  18. Overview of collective effects in the NLC main damping rings

    SciTech Connect

    Wolski, A.; de Santis, S.

    2002-05-30

    The present design for the NLC Main Damping Rings (MDRs) meets the specifications for acceptance and extracted emittance, in the limit of zero current. However, the relatively large bunch charge and moderate energy mean that a variety of collective effects can impact the beam dynamics, leading to loss of stability or increase of equilibrium emittance. These effects include intrabeam scattering, impedance from numerous sources, fast ion instability, and (in the positron ring) electron cloud. In this note, we survey the expected impact on damping ring performance from each of a number of collective effects, and discuss the priorities for future studies in this area.

  19. Measured emittance versus store time in the SLC damping ring

    SciTech Connect

    Decker, F.J.; Emma, P.; Krejcik, P.; Limberg, T.; Minty, M.; Moshammer, H.; Raubenheimer, T.; Ross, M.; Seeman, J.T.; Siemann, R.; Spence, W.; Spencer, J.; Woodley, M.

    1992-03-01

    Emittance studies at the SLC North Damping Ring led to precise measurements of the damping time using three independent methods. These measurements were done at three different locations: (1) in the ring using a fast gated video camera which allows the acquisition of the image of the synchrotron light from a single turn, (2) using the extracted beam and a single wire scanner in the ring-to-linac transport line, and (3) in the linac using four wire scanners. In addition the extracted beam emittance was studied as a function of various parameters. A significant dependence on the tune was observed.

  20. Bunching for shorter damping rings for the ILC

    SciTech Connect

    Neuffer, D.; /Fermilab

    2005-05-01

    A variant rearrangement of the bunch trains for the ILC that enables much shorter damping rings is presented. In a particular example the {approx}2820 bunches are regrouped into {approx}550 subtrains of five adjacent bunches. These subtrains are extracted from the damping rings at {approx}1.8 {micro}s intervals, obtaining the 1ms macrobunch length of the baseline TESLA collider scenario. If the baseline damping rf frequency is 325 MHz and the kicker rise and fall times are {approx}20 ns, a ring circumference of {approx}5.8km is required. Variations of the scheme could easily reduce the circumference to {approx}4km, and faster kickers could reduce it even further.

  1. Bunching for shorter damping rings for the ILC

    SciTech Connect

    Neuffer, David; /Fermilab

    2004-12-01

    A variant rearrangement of the bunch trains for the ILC that enables much shorter damping rings is presented. In a particular example the {approx}2280 bunches are regrouped into {approx}450 subtrains of five adjacent bunches. These subtrains are extracted from the damping rings at {approx}2.2 {micro}s intervals, obtaining the 1ms macrobunch length of the baseline TESLA collider scenario. If the baseline damping rf frequency is 325 MHz and the kicker rise and fall times are {approx}20 ns, a ring circumference of {approx}4.5km is required. Variations of the scheme could easily reduce the circumference to {approx}3km, and faster kickers could reduce it even further.

  2. A damping ring design for future linear colliders

    SciTech Connect

    Raubenheimer, T.O.; Gabella, W.E.; Morton, P.L.; Lee, M.J.; Rivkin, L.Z.; Ruth, R.D.

    1989-03-01

    In this paper we present a preliminary design of a damping ring for the TeV Linear Collider (TLC), a future linear collider with an energy of 1/2 to 1 TeV in the center of mass. Because of limits on the emittance, repetition rate and longitudinal impedance, we use combined function FODO cells with wigglers in insertion regions; there are approximately 22 meters of wigglers in the 155 meter ring. The ring has a normalized horizontal emittance, including the effect of intrabeam scattering, which is less than 3 /times/ 10/sup /minus/6/ and an emittance ratio of epsilon/sub x/ approx. 100epsilon/sub y/. It is designed to damp bunches for 7 vertical damping times while operating at a repetition rate of 360 Hz. Because of these requirements on the emittance and the damping per bunch, the ring operates at 1.8 GeV and is relatively large, allowing more bunches to be damped at once. 10 refs., 5 figs., 2 tabs.

  3. Recommendation for the Feasibility of more Compact LC Damping Rings

    SciTech Connect

    Pivi, M.T.F.; Wang, L.; Demma, T.; Guiducci, S.; Suetsugu, Y.; Shibata, K.; Ohmi, K.; Dugan, G.; Palmer, M.; Crittenden, J.A.; Harkay, K.; Boon, L.; Furman, M.A.; Venturini, M.; Celata, C.; Malyshev, O.B.; Papaphilippou, I.; /CERN

    2010-06-15

    As part of the international Linear Collider (ILC) collaboration, we have compared the electron cloud (EC) effect for different Damping Ring (DR) designs respectively with 6.4 km and 3.2 km circumference and investigated the feasibility of the shorter damping ring with respect to the electron cloud build-up and related beam instabilities. The studies for a 3.2 km ring were carried out with beam parameters of the ILC Low Power option. A reduced damping ring circumference has been proposed for the new ILC baseline design SB2009 [1] and would allow considerable reduction of the number of components, wiggler magnets and costs. We discuss the impact of the proposed operation of the ILC at high repetition rate 10 Hz and address the necessary modifications for the DRs. We also briefly discuss the plans for future studies including the luminosity upgrade option with shorter bunch spacing, the evaluation of mitigation techniques and the integration of the CesrTA results into the Damping Ring design.

  4. Recommendation for the Feasibility of more Compact LC Damping Rings

    SciTech Connect

    Pivi, M. T. F.; Wang, L.; Demma, T.; Guiducci, S.; Suetsugu, Y.; Fukuma, H.; Shibata, K.; Dugan, K.,G.; Palmer, M.; Crittenden, J.; Harkay, K.; Boon, L.; Furman, M. A.; Venturini, M.; Celata, C.; Malyshev, O.; Papaphilippou, I.

    2010-05-23

    As part of the international Linear Collider (ILC) collaboration, we have compared the electron cloud (EC) effect for different Damping Ring (DR) designs respectively with 6.4 km and 3.2 km circumference and investigated the feasibility of a shorter damping ring with respect to the electron cloud build-up and related beam instability. The studies for a 3.2 km ring were carried out with beam parameters of the ILC Low Power option. A reduced damping ring circumference has been proposed for the new ILC baseline design SB2009 [1] and would allow to considerably reduce the number of components, wiggler magnets and costs. We discuss the impact of the proposed operation of the ILC at high repetition rate 10 Hz and address the necessary modifications for the DRs. We also briefly discuss the plans for future studies including the luminosity upgrade option with shorter bunch spacing, the evaluation of mitigations and the integration of the CesrTA results into the Damping Ring design.

  5. Analytic estimates of coupling in damping rings

    SciTech Connect

    Raubenheimer, T.O.; Ruth, R.D.

    1989-03-01

    In this paper we present analytic formulas to estimate the vertical emittance in weakly coupled electron/positron storage rings. We consider contributions from both the vertical dispersion and linear coupling of the betatron motions. In addition to simple expressions for random misalignments and rotations of the magnets, formulas are presented to calculate the vertical emittance blowup due to orbit distortions. The orbit distortions are assumed to be caused by random misalignments, but because the closed orbit is correlated from point to point, the effects must be treated differently. We consider only corrected orbits. Finally, the analytic expressions are compared with computer simulations of storage rings with random misalignments. 6 refs., 3 figs.

  6. Compensating the unequal bunch spacing in the NLC damping rings

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    Bane, K.L.F.; Wilson, P.B.; Kubo, K.

    1996-06-01

    The damping rings of the Next Linear Collider (NLC), at any given time, will contain four trains of 90 bunches each. Within each train the bunches populate adjacent buckets and between trains there is a gap that extends over 43 buckets. A consequence of an uneven filling scheme is that within each train the synchronous phase will vary from bunch to bunch. In the NLC after extraction the beam enters the bunch compressor and then the X-band linac. The phase variation in the ring, if uncompensated, will lead to a phase variation in the X-band linac which, in turn, will result in an unacceptable spread in the final energy of the individual bunches of a train. The synchronous phase variation, however, can be compensated, either in the damping ring itself or in the bunch compressor that follows. The subject of this paper is compensation in the damping ring. In this report we begin by finding the synchronous phase variation in damping rings with bunch trains and gaps of arbitrary length. These results are then applied to the parameters of the NLC damping rings. Finally, we study two methods of compensating this phase variation: in the first method two passive subharmonic cavities are employed, and in the second the klystron output is varied as a function of time. We find that, for the NLC, a nominal phase variation of 6 degrees within a train can be reduced by almost an order of magnitude by either method of compensation, with the cost of the second method being an extra 10% in output power capability of the klystron.

  7. Injection and Extraction Lines for the ILC Damping Rings

    SciTech Connect

    Reichel, Ina

    2007-06-20

    The current design for the injection and extraction linesintoand out of the ILC Damping Rings is presented as well as the designfor the abort line. Due to changes of the geometric boundary conditionsby other subsystems of the ILC, a modular approach has been used to beable to respond to recurring layout changes whilereusing previouslydesigned parts.

  8. A possible redesign of the SLAC SLC damping rings

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    Raubenheimer, T.O.; Early, R.; Limberg, T.; Moshammer, H.; Spencer, J.

    1993-04-01

    We describe a possible replacement for the SLC damping rings that would generate beams with normalized horizontal emittances of {gamma}{epsilon}{sub x} = 9 {times} 10{sup {minus}6} m-rad when uncoupled; this is more than three times smaller than that generated by the current rings. The primary difference between the new design and the current ring is the arc cell structure; the insertion regions, the kickers, and the RF are essentially unchanged. The new cell uses a single combined function bending magnet, roughly 70 cm in length, to replace the two bends, defocusing quadrupole, and defocusing sextupoles in the current FODO cell; the focusing quadrupole and sextupoles, used in the current cell, are also used in the new structure. The length of the new cell is identical to that of the current cell and thus nine of these new cells would simply replace the nine FODO cells in each arc of the rings.

  9. CSR in the SuperKEKB Damping Ring

    SciTech Connect

    Zhou, Demin; Abe, Tetsuo; Ikeda, Hitomi; Kikuchi, Mitsuo; Ohmi, Kazuhito; Oide, Katsunobu; Shibata, Kyo; Tobiyama, Makoto; Stupakov, Gennady; /SLAC

    2012-07-06

    Coherent synchrotron radiation (CSR) is generated when a bunched beam traverses a dipole magnet or a wiggler/undulator. It can degrade the beam quality in both storage rings and linacs through enhancing the beam energy spread and lengthening the bunch length, even cause single-bunch microwave instabilities. Using several methods, CSR impedances in the positron damping ring (DR) of the SuperKEKB which is under design were calculated. From the impedances due to CSR, resistive wall and various vacuum components, quasi-Green function wake potentials were constructed and used in simulations of Particle-In-Cell (PIC) tracking. We present the CSR related results in this paper.

  10. Analysis of KEK-ATF optics and coupling using LOCO

    SciTech Connect

    Wolski, Andrzej; Woodley, Mark D.; Nelson, Janice; Ross, Marc C.

    2004-06-30

    LOCO is a computer code for analysis of the linear optics in a storage ring based on the closed orbit response to steering magnets. The analysis provides information on focusing errors, BPM gain and rotation errors, and local coupling. Here, we discuss the details of the LOCO implementation at the KEK-ATF Damping Ring, and report the initial results. Some of the information obtained, for example on the BPM gain and coupling errors, has not previously been determined. We discuss the possibility of using the data provided by the LOCO analysis to reduce the vertical emittance of the ATF beam.

  11. Analysis of KEK-ATF Optics and Coupling Using LOCO

    SciTech Connect

    Wolski, A

    2004-08-04

    LOCO is a computer code for analysis of the linear optics in a storage ring based on the closed orbit response to steering magnets. The analysis provides information on focusing errors, BPM gain and rotation errors, and local coupling. Here, the authors discuss the details of the LOCO implementation at the KEK-ATF Damping Ring and report the initial results. Some of the information obtained, for example on the BPM gain and coupling errors, had not previously been determined. They discuss the possibility of using the data provided by the LOCO analysis to reduce the vertical emittance of the ATF beam.

  12. Configuration Studies and Recommendations for the ILC DampingRings

    SciTech Connect

    Wolski, Andrzej; Gao, Jie; Guiducci, Susanna

    2006-02-04

    We describe the results of studies comparing differentoptions for the baseline configuration of the ILC damping rings. Theprincipal configuration decisions apply to the circumference, beamenergy, lattice type, and technology options for key components,including the injection/extraction kickers and the damping wigglers. Toarrive at our recommended configuration, we performed detailed studies ofa range of lattices representing a variety of different configurationoptions; these lattices are described in Chapter 2. The results of thevarious studies are reported in chapters covering issues of beamdynamics, technical subsystems, costs, and commissioning, reliability andupgradeability. Our detailed recommendations for the baselineconfiguration are given in Chapter 7, where we also outline furtherresearch and development that is needed before a machine using ourrecommended configuration can be built and operated successfully. In thesame chapter, we suggest possible alternatives to the baselineconfiguration.

  13. Configuration Studies and Recommendations for the ILC DampingRings

    SciTech Connect

    Wolski, Andrzej; Gao, Jie; Guiducci, Susanna

    2006-02-04

    We describe the results of studies comparing different options for the baseline configuration of the ILC damping rings. The principal configuration decisions apply to the circumference, beam energy, lattice type, and technology options for key components, including the injection/extraction kickers and the damping wigglers. To arrive at our recommended configuration, we performed detailed studies of a range of lattices representing a variety of different configuration options; these lattices are described in Chapter 2. The results of the various studies are reported in chapters covering issues of beam dynamics, technical subsystems, costs, and commissioning, reliability and upgrade ability. Our detailed recommendations for the baseline configuration are given in Chapter 7, where we also outline further research and development that is needed before a machine using our recommended configuration can be built and operated successfully. In the same chapter, we suggest possible alternatives to the baseline configuration.

  14. Proposed emittance upgrade for the SLC damping rings

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    Early, R.; Limberg, T.; Moshammer, H.; Raubenheimer, T.; Skarpaas, K.; Spencer, J.

    1994-06-01

    One way to improve luminosity is to reduce transverse emittance by changing damping partitions. We consider the options in relation to the constraints. Besides modifications of the basic DR configuration the options include closed-orbit offsets in the quadrupoles, addition of strong multipoles and replacement of existing rectangular nosepieces on the dipoles (shim angles {phi}{equivalent_to}{theta}/2) with rotatable inserts. Measurements indicate the possibility of dynamically tuning {phi}>{ge}45{degrees} with decreases in {tau}{sub x} and {epsilon}{sub x} of {ge}50%. We discuss damping mechanisms to motivate the desirable field characteristics as well as nonlinear contours to cancel dipole harmonic errors (B>2T here) or to provide chromatic corrections. Such inserts could also be used to make cheaper, more compact rings with better impedance by reducing the number of conventional multipoles without impairing the stability. Estimated hardware costs are 250$/dipole end or multipole equivalent.

  15. Characterization of the International Linear Collider damping ring optics

    NASA Astrophysics Data System (ADS)

    Shanks, J.; Rubin, D. L.; Sagan, D.

    2014-10-01

    A method is presented for characterizing the emittance dilution and dynamic aperture for an arbitrary closed lattice that includes guide field magnet errors, multipole errors and misalignments. This method, developed and tested at the Cornell Electron Storage Ring Test Accelerator (CesrTA), has been applied to the damping ring lattice for the International Linear Collider (ILC). The effectiveness of beam based emittance tuning is limited by beam position monitor (BPM) measurement errors, number of corrector magnets and their placement, and correction algorithm. The specifications for damping ring magnet alignment, multipole errors, number of BPMs, and precision in BPM measurements are shown to be consistent with the required emittances and dynamic aperture. The methodology is then used to determine the minimum number of position monitors that is required to achieve the emittance targets, and how that minimum depends on the location of the BPMs. Similarly, the maximum tolerable multipole errors are evaluated. Finally, the robustness of each BPM configuration with respect to random failures is explored.

  16. Impedance and instabilities in the NLC damping rings

    SciTech Connect

    Corlett,J.; Li, D.; Pivi, M.; Rimmer, R.; DeSantis, S.; Wolski, A.; Novokhatski,A.; Ng, C.

    2001-06-12

    We report on impedance calculations and single-bunch and multi-bunch instabilities in the NLC damping rings. Preliminary designs of vacuum chambers and major components have addressed beam impedance issues, with the desire to increase instability current thresholds and reducing growth rates. MAFIA calculations of short-range and long-range wakefields have allowed computations of growth rates and thresholds, which are presented here. Resistive wall instability dominates long-range effects, and requires a broadband feedback system to control coupled-bunch motion. Growth rates are within the range addressable by current feedback system technologies. Single-bunch instability thresholds are safely above nominal operating current.

  17. Pulse shape adjustment for the SLC damping ring kickers

    SciTech Connect

    Mattison, T.; Cassel, R.; Donaldson, A.; Fischer, H.; Gough, D.

    1991-05-01

    The difficulties with damping ring kickers that prevented operation of the SLAC Linear Collider in full multiple bunch mode have been overcome by shaping the current pulse to compensate for imperfections in the magnets. The risetime was improved by a peaking capacitor, with a tunable inductor to provide a locally flat pulse. The pulse was flattened by an adjustable droop inductor. Fine adjustment was provided by pulse forming line tuners driven by stepping motors. Further risetime improvement will be obtained by a saturating ferrite pulse sharpener. 4 refs., 3 figs.

  18. ATF2 Summary and Status

    SciTech Connect

    Alabau-Gonzalvo, J.; Alabau Pons, M.; Amann, J.; Angal-Kalinin, D.; Apsimon, R.; Araki, S.; Aryshev, A.; Bai, S.; Bambade, P.; Bellomo, P.; Bett, D.; Blair, G.; Blanch, C.; Bolzon, B.; Boogert, S.; Boorman, G.; Burrows, P.; Christian, G.; Coe, P.; Constance, B.; Corner, L.; /Oxford U., JAI /Oxford U., JAI /CERN /Oxford U., JAI /DESY /Valencia U., IFIC /SLAC /KEK, Tsukuba /Beijing, Inst. High Energy Phys. /ESIA, Annecy /Fermilab /Ecole Polytechnique /KEK, Tsukuba /Kyungpook Natl. U. /KEK, Tsukuba /Pohang Accelerator Lab. /Pohang Accelerator Lab. /Kyoto U., Inst. Chem. Res. /ESIA, Annecy /Cockcroft Inst. Accel. Sci. Tech. /Tokyo U. /Oxford U., JAI /Kyungpook Natl. U. /Kyungpook Natl. U. /Pohang Accelerator Lab. /Kyungpook Natl. U. /Tokyo U. /KEK, Tsukuba /KEK, Tsukuba /KEK, Tsukuba /SLAC /University Coll. London /KEK, Tsukuba /SLAC /Oxford U., JAI /KEK, Tsukuba /Tokyo U. /SLAC /Oxford U., JAI /Tohoku U. /KEK, Tsukuba /Tokyo U. /Pohang Accelerator Lab. /Brookhaven /SLAC /Oxford U., JAI /SLAC /SLAC /CERN /Oxford U., JAI /Orsay, LAL /Fermilab /Tohoku U. /Manchester U. /CERN /Oxford U., JAI /SLAC /SLAC /SLAC /Tokyo U. /KEK, Tsukuba /Oxford U., JAI /Hiroshima U. /KEK, Tsukuba /KEK, Tsukuba /CERN /KEK, Tsukuba /Oxford U., JAI /Ecole Polytechnique /Oxford U., JAI /Beijing, Inst. High Energy Phys. /SLAC /Oxford U., JAI /Fermilab /SLAC /SLAC /Liverpool U. /SLAC /Tokyo U. /Tokyo U. /Tokyo U. /Tokyo U. /KEK, Tsukuba /SLAC /CERN

    2012-04-19

    The Accelerator Test Facility (ATF) [1] at KEK was originally constructed as a Damping Ring (DR) demonstration facility for the next generation of electron linear colliders. It consists of a 1.3 GeV s-band linac and a 136m circumference damping ring which can routinely generate 1.6 nC electron bunches with very small vertical emittances (<10pm.rad). Completed in December of 2008, a new extraction line and final focus optics was built, this is referred to as ATF2 [2]. The location of ATF in-relation to the KEK site can be seen in Figure 1. ATF is a general-purpose facility for accelerator physics related R and D activities, with approximately 50% of the available beam time currently devoted to the ATF2 program. The ATF2 extraction line (EXT) and final focus system (FFS) extends about 90 meters from the DR extraction point, a sketch of this system can be seen in figure 2, along with the location of key instrumentation. The primary purpose of the ATF2 beam line is to demonstrate a new style of magnetic focusing optics intended for use at future linear colliders, such as ILC or CLIC based on a local chromaticity correction scheme. The primary goal of the collaboration is to demonstrate the ability of this optics to focus an electron beam of similar emittance to the ILC using a FFS of the same design chromaticity. A comparison of some of the ATF2 design parameters with those of the ILC and CLIC are shown in table 1. With the 1.3 GeV beam energy of ATF2, the target spot size to prove the operability of this optics for ILC is 37nm. This is referred to as 'Goal 1'. In addition, Goal 1 requires the demonstration of stable operations with such a beam size at the Interaction Point (IP). 'Goal 2' is to demonstrate nm-level stability with respect to the IP beam monitors, such as is required for stable beam collisions at ILC or CLIC. A further goal is to attempt to increase the chromaticity of the final focus optics, reducing the IP beta functions to a level where the system

  19. Resistive-Wall Instability in the Damping Rings of the ILC

    SciTech Connect

    Wang, L.; Bane, K.L.F.; Raubenheimer, T.; Ross, M.; /SLAC

    2006-07-05

    In the damping rings of the International Linear Collider (ILC), the resistive-wall instability is one of the dominant transverse instabilities. This instability directly influences the choice of material and aperture of the vacuum pipe, and the parameters of the transverse feedback system. This paper investigates the resistive-wall instabilities in an ILC damping ring under various conditions of beam pipe material, aperture, and fill pattern.

  20. NLC Damping Ring Lattice Design Using TME Cells and Wigglers (LCC-0037)

    SciTech Connect

    Emma, P

    2004-03-23

    The design process is described for the damping rings of the Next Linear Collider (NLC) [1]. While the main (e{sup -}/e{sup +}) damping ring is the primary subject, some reference to the (e{sup +}) pre-damping ring is also included. An attempt is made to be systematic in the design, given the emittance and repetition rate requirements of the collider, in conjunction with the beam quality from the injector systems. In addition, qualitative efforts are made to minimize the cost and maximize the dynamic aperture. The approximate interdependencies of the various lattice parameters are derived and arguments for the parameter choices are presented. The rings are a race-track design with wiggler insertions and Theoretical Minimum Emittance (TME) cells. Collective effects and dynamic aperture calculations, while studied in previous designs [1], are not included here.

  1. Transverse Field Profile of the NLC Damping Rings Eletromagnet Wiggler (LCC-0038)

    SciTech Connect

    Ross, M

    2004-03-19

    The primary effort for damping ring wiggler studies has been to develop a credible radiation hard electromagnet wiggler conceptual design that meets NLC main electron and positron damping ring physics requirements [1]. Based upon an early assessment of requirements, a hybrid magnet similar to existing designs satisfies basic requirements. However, radiation damage is potentially a serious problem for the Nd-Fe-B permanent magnet material, and cost remains an issue for samarium cobalt magnets. Superconducting magnet designs have not been pursued due to their increased complexity and our unfamiliarity with the technology. Having produced and developed an electromagnet design, we now find that the transverse field roll-off is severe, and recognizing similar experience with beamline 11 at SSRL we believe that the resulting beam quality will not meet the damping ring requirements. We therefore propose, in parallel with more detailed optics studies of the wiggler field requirements, to revisit the hybrid permanent magnet design.

  2. An active feedback system to control synchrotron oscillations in the SLC Damping Rings

    SciTech Connect

    Corredoura, P.L.; Pellegrin, J.L.; Schwarz, H.D.; Sheppard, J.C.

    1989-03-01

    Initially the SLC Damping Rings accomplished Robinson instability damping by operating the RF accelerating cavities slightly detuned. In order to be able to run the cavities tuned and achieve damping for Robinson instability and synchrotron oscillations at injection an active feedback system has been developed. This paper describes the theoretical basis for the feedback system and the development of the hardware. Extensive measurements of the loop response including stored beam were performed. Overall performance of the system is also reported. 3 refs., 6 figs.

  3. Investigation into electron cloud effects in the International Linear Collider positron damping ring

    SciTech Connect

    Crittenden, J.A.; Conway, J.; Dugan, G.F.; Palmer, M.A.; Rubin, D.L.; Shanks, J.; Sonnad, K.G.; Boon, L.; Harkay, K.; Ishibashi, T.; Furman, M.A.; Guiducci, S.; Pivi, M.T.F.; Wang, L.; Crittenden, J.A.; Conway, J.; Dugan, G.F.; Palmer, M.A.; Rubin, D.L.; Shanks, J.; Sonnad, K.G.; Boon, L.; Harkay, K.; Ishibashi, T.; Furman, M.A.; Guiducci, S.; Pivi, M.T.F.; Wang, L.

    2014-02-28

    We report modeling results for electron cloud buildup and instability in the International Linear Collider positron damping ring. Updated optics, wiggler magnets, and vacuum chamber designs have recently been developed for the 5 GeV, 3.2-km racetrack layout. An analysis of the synchrotron radiation profile around the ring has been performed, including the effects of diffuse and specular photon scattering on the interior surfaces of the vacuum chamber. The results provide input to the cloud buildup simulations for the various magnetic field regions of the ring. The modeled cloud densities thus obtained are used in the instability threshold calculations. We conclude that the mitigation techniques employed in this model will suffice to allow operation of the damping ring at the design operational specifications

  4. Longitudinal single-bunch instabilities in the NLC main damping rings

    SciTech Connect

    Venturini, Marco

    2004-05-10

    Because of tight requirements on beam quality longitudinal single-bunch instabilities are a serious concern for the damping rings of the next generation of linear colliders. Unlike multi-bunch instabilities they cannot be damped using feed-back systems and need to be avoided altogether. We present an analysis of these instabilities for the current Feb. 03 NLC main damping ring design, with attention paid to coherent synchrotron radiation and vacuum chamber effects, with the latter including the main components (RF cavities, BPM's, and resistive wall). The study is carried out by solving the Vlasov-Fokker-Planck equation for the longitudinal motion numerically. Comparison is made, whenever possible, with linear theory. We find that collective effects are dominated by coherent synchrotron radiation and estimate the instability threshold to be safely above 6 times the design current.

  5. An ultimate storage ring lattice with vertical emittance generated by damping wigglers

    SciTech Connect

    Huang, Xiaobiao

    2015-01-06

    We discuss the approach of generating round beams for ultimate storage rings using vertical damping wigglers (with horizontal magnetic field). The vertical damping wigglers provide damping and excite vertical emittance. This eliminates the need to generate large linear coupling that is impractical with traditional off-axis injection. We use a PEP-X compatible lattice to demonstrate the approach. This lattice uses separate quadrupole and sextupole magnets with realistic gradient strengths. Intrabeam scattering effects are calculated. As a result, the horizontal and vertical emittances are 22.3 pm and 10.3 pm, respectively, for a 200 mA, 4.5 GeV beam, with a vertical damping wiggler of a total length of 90 m, a peak field of 1.5 T and a wiggler period of 100 mm.

  6. On the Linear Damping Relation for Density Waves in Saturn’s Rings

    NASA Astrophysics Data System (ADS)

    Schmidt, Jürgen; Colwell, Joshua E.; Lehmann, Marius; Marouf, Essam A.; Salo, Heikki; Spahn, Frank; Tiscareno, Matthew S.

    2016-06-01

    We revisit the equation for viscous damping of density waves derived from linearized theory and show that the damping is not only determined by the magnitudes of shear and bulk viscosity. Modifications arise from the dependence of the viscosity on the ring’s surface mass density. This was noted more than 30 years ago by Goldreich & Tremaine (1978b). Still, to date the consequences have not been explored. In the literature these terms have been neglected throughout when fitting the rings’ viscosity from observations of wave damping. Therefore, one must suspect that these viscosities, as well as the dispersion velocities inferred from them, suffer from systematic bias, which might be small or significant, depending on the local conditions in the ring. We show that the modified damping formula, to linear order, is related to the stability threshold for viscous overstability and argue that the appearance of density waves may be altered by this instability.

  7. Operational Experiences Tuning the ATF2 Final Focus Optics Towards Obtaining a 37nm Electron Beam IP Spot Size

    SciTech Connect

    White, Glen; Seryi, Andrei; Woodley, Mark; Bai, Sha; Bambade, Philip; Renier, Yves; Bolzon, Benoit; Kamiya, Yoshio; Komamiya, Sachio; Oroku, Masahiro; Yamaguchi, Yohei; Yamanaka, Takashi; Kubo, Kiyoshi; Kuroda, Shigeru; Okugi, Toshiyuki; Tauchi, Toshiaki; Marin, Eduardo; /CERN

    2012-07-06

    The primary aim of the ATF2 research accelerator is to test a scaled version of the final focus optics planned for use in next-generation linear lepton colliders. ATF2 consists of a 1.3 GeV linac, damping ring providing low-emittance electron beams (< 12pm in the vertical plane), extraction line and final focus optics. The design details of the final focus optics and implementation at ATF2 are presented elsewhere. The ATF2 accelerator is currently being commissioned, with a staged approach to achieving the design IP spot size. It is expected that as we implement more demanding optics and reduce the vertical beta function at the IP, the tuning becomes more difficult and takes longer. We present here a description of the implementation of the tuning procedures and describe operational experiences and performances.

  8. Design and system integration of the superconducting wiggler magnets for the Compact Linear Collider damping rings

    NASA Astrophysics Data System (ADS)

    Schoerling, Daniel; Antoniou, Fanouria; Bernhard, Axel; Bragin, Alexey; Karppinen, Mikko; Maccaferri, Remo; Mezentsev, Nikolay; Papaphilippou, Yannis; Peiffer, Peter; Rossmanith, Robert; Rumolo, Giovanni; Russenschuck, Stephan; Vobly, Pavel; Zolotarev, Konstantin

    2012-04-01

    To achieve high luminosity at the collision point of the Compact Linear Collider (CLIC), the normalized horizontal and vertical emittances of the electron and positron beams must be reduced to 500 and 4 nm before the beams enter the 1.5 TeV linear accelerators. An effective way to accomplish ultralow emittances with only small effects on the electron polarization is using damping rings operating at 2.86 GeV equipped with superconducting wiggler magnets. This paper describes a technical design concept for the CLIC damping wigglers.

  9. Simulations of the Electron Cloud Builld Up and Instabilities for Various ILC Damping Ring Configurations

    SciTech Connect

    Pivi, Mauro; Raubenheimer, Tor O.; Wang, Lanfa; Ohmi, Kazuhito; Wanzenberg, Rainer; Wolski, Andrzej; /Liverpool U. /Cockcroft Inst. Accel. Sci. Tech.

    2007-03-12

    In the beam pipe of the positron damping ring of the International Linear Collider (ILC), an electron cloud may be first produced by photoelectrons and ionization of residual gases and then increased by the secondary emission process. This paper reports the assessment of electron cloud effects in a number of configuration options for the ILC baseline configuration. Careful estimates were made of the secondary electron yield (sometimes in the literature also referred as secondary emission yield SEY or {delta}, with a peak value {delta}{sub max}) threshold for electron cloud build-up, and the related single- and coupled-bunch instabilities, as a function of beam current and surface properties for a variety of optics designs. When the configuration for the ILC damping rings was chosen at the end of 2005, the results from these studies were important considerations. On the basis of the joint theoretical and experimental work, the baseline configuration currently specifies a pair of 6 km damping rings for the positron beam, to mitigate the effects of the electron cloud that could present difficulties in a single 6 km ring. However, since mitigation techniques are now estimated to be sufficiently mature, a reduced single 6-km circumference is presently under consideration so as to reduce costs.

  10. Active Damping of the E-P Instability at the Los Alamos Proton Storage Ring

    SciTech Connect

    Macek, R.J.; Assadi, S.; Byrd, J.M.; Deibele, C.E.; Henderson, S.D.; Lee, S.Y.; McCrady, R.C.; Pivi, M.F.T.; Plum, M.A.; Walbridge, S.B.; Zaugg, T.J.; /Los Alamos

    2008-03-17

    A prototype of an analog, transverse (vertical) feedback system for active damping of the two-stream (e-p) instability has been developed and successfully tested at the Los Alamos Proton Storage Ring (PSR). This system was able to improve the instability threshold by approximately 30% (as measured by the change in RF buncher voltage at instability threshold). The feedback system configuration, setup procedures, and optimization of performance are described. Results of several experimental tests of system performance are presented including observations of instability threshold improvement and grow-damp experiments, which yield estimates of instability growth and damping rates. A major effort was undertaken to identify and study several factors limiting system performance. Evidence obtained from these tests suggests that performance of the prototype was limited by higher instability growth rates arising from beam leakage into the gap at lower RF buncher voltage and the onset of instability in the horizontal plane, which had no feedback.

  11. Active damping of the e-p instability at the Los Alamos Proton Storage Ring

    SciTech Connect

    Macek, R. J.; Assadi, S.; Byrd, J. M.; Deibele, C. E.; Henderson, S. D.; Lee, S. Y.; McCrady, R. C.; Pivi, M. F. T.; Plum, M. A.; Walbridge, S. B.; Zaugg, T. J.

    2007-12-15

    A prototype of an analog, transverse (vertical) feedback system for active damping of the two-stream (e-p) instability has been developed and successfully tested at the Los Alamos Proton Storage Ring (PSR). This system was able to improve the instability threshold by approximately 30% (as measured by the change in RF buncher voltage at instability threshold). The feedback system configuration, setup procedures, and optimization of performance are described. Results of several experimental tests of system performance are presented including observations of instability threshold improvement and grow-damp experiments, which yield estimates of instability growth and damping rates. A major effort was undertaken to identify and study several factors limiting system performance. Evidence obtained from these tests suggests that performance of the prototype was limited by higher instability growth rates arising from beam leakage into the gap at lower RF buncher voltage and the onset of instability in the horizontal plane, which had no feedback.

  12. Conceptual design of International Linear Collider damping ring wiggler based on a hybrid technology.

    PubMed

    Smirnov, Alexei V

    2009-01-01

    A magnetic design of a failure-free damping ring wiggler with 100% duty factor, 55.6 mm gap, and field exceeding 1.6 T is proposed. The insertion device is based on permanent magnets and specially shimmed poles that capable to meet the requirements of the International Linear Collider (ILC) damping rings (including positron one), with field quality and gap comparable to that projected for ILC using superconducting wiggler. Performance improvement of the modified hybrid design is attained due to yoke reduction down to six separated strips and maximized packaging of the midgrade (or high-grade) parallelepiped magnet blocks surrounding the Permendur poles. Economical efficiency is demonstrated on the base of calculated amount of magnetic material. A cryogenic variant of the hybrid design may certainly provide good sustainability to harsh radiation environment and further enhancement of the design efficiency within the state of the art. PMID:19191428

  13. Conceptual design of International Linear Collider damping ring wiggler based on a hybrid technology

    NASA Astrophysics Data System (ADS)

    Smirnov, Alexei V.

    2009-01-01

    A magnetic design of a failure-free damping ring wiggler with 100% duty factor, 55.6 mm gap, and field exceeding 1.6 T is proposed. The insertion device is based on permanent magnets and specially shimmed poles that capable to meet the requirements of the International Linear Collider (ILC) damping rings (including positron one), with field quality and gap comparable to that projected for ILC using superconducting wiggler. Performance improvement of the modified hybrid design is attained due to yoke reduction down to six separated strips and maximized packaging of the midgrade (or high-grade) parallelepiped magnet blocks surrounding the Permendur poles. Economical efficiency is demonstrated on the base of calculated amount of magnetic material. A cryogenic variant of the hybrid design may certainly provide good sustainability to harsh radiation environment and further enhancement of the design efficiency within the state of the art.

  14. Conceptual design of International Linear Collider damping ring wiggler based on a hybrid technology

    SciTech Connect

    Smirnov, Alexei V.

    2009-01-15

    A magnetic design of a failure-free damping ring wiggler with 100% duty factor, 55.6 mm gap, and field exceeding 1.6 T is proposed. The insertion device is based on permanent magnets and specially shimmed poles that capable to meet the requirements of the International Linear Collider (ILC) damping rings (including positron one), with field quality and gap comparable to that projected for ILC using superconducting wiggler. Performance improvement of the modified hybrid design is attained due to yoke reduction down to six separated strips and maximized packaging of the midgrade (or high-grade) parallelepiped magnet blocks surrounding the Permendur poles. Economical efficiency is demonstrated on the base of calculated amount of magnetic material. A cryogenic variant of the hybrid design may certainly provide good sustainability to harsh radiation environment and further enhancement of the design efficiency within the state of the art.

  15. Landau damping effects and evolutions of energy spread in small isochronous ring

    SciTech Connect

    Li, Yingjie; Wang, Langfa; Lin, Fanglei

    2014-11-01

    This paper presents the Landau damping effects on the microwave instability of a coasting long bunch in an isochronous ring due to finite energy spread and emittance. Our two-dimensional (2D) dispersion relation gives more accurate predictions of the microwave instability growth rates of short-wavelength perturbations than the conventional 1D formula. The long-term evolution of energy spread is also studied by measurements and simulations.

  16. A modified post damping ring bunch compressor beamline for the TESLA linear collider

    SciTech Connect

    Philippe R.-G. Piot; Winfried Decking

    2004-03-23

    We propose a modified bunch compressor beamline, downstream of the damping ring, for the TESLA linear collider. This modified beamline uses a third harmonic radio-frequency section based on the 3.9 GHz superconducting cavity under development at Fermilab. In our design the beam deceleration is about {approx}50 MeV instead of {approx}450 MeV in the original design proposed.

  17. The Conversion and operation of the Cornell electron storage ring as a test accelerator (cesrta) for damping rings research and development

    SciTech Connect

    Palmer, M.A.; Alexander, J.; Byrd, J.; Celata, C.M.; Corlett, J.; De Santis, S.; Furman, M.; Jackson, A.; Kraft, R.; Munson, D.; Penn, G.; Plate, D.; Rawlins, A.; Venturini, M.; Zisman, M.; Billing, M.; Calvey, J.; Chapman, S.; Codner, G.; Conolly, C.; Crittenden, J.; Dobbins, J.; Dugan, G.; Fontes, E.; Forster, M.; Gallagher, R.; Gray, S.; Greenwald, S.; Hartill, D.; Hopkins, W.; Kandaswamy, J.; Kreinick, D.; Li, Y.; Liu, X.; Livezey, J.; Lyndaker, A.; Medjidzade, V.; Meller, R.; Peck, S.; Peterson, D.; Rendina, M.; Revesz, P.; Rice, D.; Rider, N.; Rubin, D.; Sagan, D.; Savino, J.; Seeley, R.; Sexton, J.; Shanks, J.; Sikora, J.; Smolenski, K.; Strohman, C.; Temnykh, A.; tigner, M.; Whitney, W.; Williams, H.; Vishniakou, S.; Wilkens, T.; Harkay, K.; Holtzapple, R.; Smith, E.; Jones, J.; Wolski, A.; He, Y.; Ross, M.; Tan, C.Y.; Zwaska, R.; Flanagan, J.; Jain, P.; Kanazawa, K.; Ohmi, K.; Sakai, H.; Shibata, K.; Suetsugu, Y.; Kharakh, D.; Pivi, M.; Wang, L.

    2009-05-01

    In March of 2008, the Cornell Electron Storage Ring (CESR) concluded twenty eight years of colliding beam operations for the CLEO high energy physics experiment. We have reconfigured CESR as an ultra low emittance damping ring for use as a test accelerator (CesrTA) for International Linear Collider (ILC) damping ring R&D. The primary goals of the CesrTA program are to achieve a beam emittance approaching that of the ILC Damping Rings with a positron beam, to investigate the interaction of the electron cloud with both low emittance positron and electron beams, to explore methods to suppress the electron cloud, and to develop suitable advanced instrumentation required for these experimental studies (in particular a fast x-ray beam size monitor capable of single pass measurements of individual bunches). We report on progress with the CESR conversion activities, the status and schedule for the experimental program, and the first experimental results that have been obtained.

  18. Development of an Adder-Topology ILC Damping Ring Kicker Modulator

    SciTech Connect

    Tang, Tao; Burkhart, Craig; /SLAC

    2009-05-08

    The ILC damping ring injection and extraction kickers will require high availability modulators that can deliver {+-}5 kV pulses into 50 {Omega} with a 2 ns flattop ({approx}1 ns rise and fall time) at up to 6 MHz. An effort is underway at SLAC National Accelerator Laboratory to meet these requirements using a transmission line adder topology to combine the output of an array of {approx}1 kV modules. The modules employ an ultra-fast hybrid MOSFET/driver that can switch 33 A in 1.2 ns. Experimental results for a scale adder structure are presented.

  19. Study of Abnormal Vertical Emittance Growth in ATF Extraction Line

    SciTech Connect

    Alabau, M.; Faus-Golfe, A.; Alabau, M.; Bambade, P.; Brossard, J.; Le Meur, G.; Rimbault, C.; Touze, F.; Angal-Kalinin, D.; Jones, J.K.; Appleby, R.; Scarfe, A.; Kuroda, S.; White, G.R.; Woodley, M.; Zimmermann, F.; /CERN

    2011-11-04

    Since several years, the vertical beam emittance measured in the Extraction Line (EXT) of the Accelerator Test Facility (ATF) at KEK, that will transport the electron beam from the ATF Damping Ring (DR) to the future ATF2 Final Focus beam line, is significantly larger than the emittance measured in the DR itself, and there are indications that it grows rapidly with increasing beam intensity. This longstanding problem has motivated studies of possible sources of this anomalous emittance growth. One possible contribution is non-linear magnetic fields in the extraction region experimented by the beam while passing off-axis through magnets of the DR during the extraction process. In this paper, simulations of the emittance growth are presented and compared to observations. These simulations include the effects of predicted non-linear field errors in the shared DR magnets and orbit displacements from the reference orbit in the extraction region. Results of recent measurements using closed orbit bumps to probe the relation between the extraction trajectory and the anomalous emittance growth are also presented.

  20. A coupled-bunch ion instability in the SLC electron damping ring

    SciTech Connect

    Krejcik, P.

    1997-05-01

    Under abnormally poor vacuum conditions in the electron damping ring, described in a companion paper at this conference, an emittance blowup was observed related to a transverse instability. The instability was only observed with two bunches in the ring, and could not be seen with single bunch operation, even at the same total beam current as for the two bunches. Measurements of the transverse Beam Transfer Function indicate strong coupling between the two bunches when ions are present. This is described in terms of a simple model where the oscillating ion cloud provides the coupling between the two bunches. The frequency shift due to coupling can also cause the ion cloud to become unstable, and disappear, when the betatron tune of the electron bunch is just below the 1/2 integer.

  1. Electron Cloud in the Wigglers of the Positron Damping Ring of the International Linear Collider

    SciTech Connect

    Wang, L.; /SLAC

    2007-07-06

    The ILC positron damping ring comprises hundreds of meters of wiggler sections, where many more photons than in the arcs are emitted, and with the smallest beampipe aperture of the ring. A significant electron-cloud density can therefore be accumulated via photo-emission and via beam-induced multipacting. In field-free regions the electron-cloud build up may be suppressed by adding weak solenoid fields, but the electron cloud remaining in the wigglers as well as in the arc dipole magnets can still drive single-bunch and multi-bunch beam instabilities. This paper studies the electron-cloud formation in an ILC wiggler section for various scenarios, as well as its character, and possible mitigation schemes.

  2. Novel Slide-Ring Material/Natural Rubber Composites with High Damping Property.

    PubMed

    Wang, Wencai; Zhao, Detao; Yang, Jingna; Nishi, Toshio; Ito, Kohzo; Zhao, Xiuying; Zhang, Liqun

    2016-01-01

    A novel class of polymers called "slide-ring" (SR) materials with slideable junctions were used for high damping composites for the first time. The SR acts as the high damping phase dispersed in the natural rubber (NR) matrix, and epoxidized natural rubber (ENR) acts as the compatibilizer. The morphological, structural, and mechanical properties of the composites were investigated by atomic force microscope (AFM), transmission electron microscope (TEM), dynamic mechanical thermal analyzer (DMTA), rubber processing analyzer (RPA), and tensile tester. AFM and TEM results showed that the SR phase was uniformly dispersed in the composites, in a small size that is a function of ENR. DMTA and RPA results showed that the damping factor of the composites is much higher than that of NR, especially at room temperatures. Stretch hysteresis was used to study the energy dissipation of the composites at large strains. The results showed that SR and ENR can significantly improve the dissipation efficiency at strains lower than 200% strain. Wide-angle X-ray diffraction was used to study the strain-induced crystallization of the composites. The results indicated that the impact of the SR on the crystallization of NR is mitigated by the insulating effect of ENR. PMID:26949077

  3. RF cavity R&D at LBNL for the NLC damping rings, FY1999

    SciTech Connect

    Rimmer, R.A.; Corlett, J.N.; Koehler, G.; Li, D.; Hartman, N.; Rasson, J.; Saleh, T.

    1999-11-01

    This report contains a summary of the R&D activities at LBNL on RF cavities for the NLC damping rings during fiscal year19999. These activities include the optimization of the RF design for both efficiency and damping of higher-order (HOMs), by systematic study of the cavity profile, the effect of the beam pipe diameter, nosecone angle and gap, the cross section and position of the HOM damping waveguides and the coupler. The effect of the shape of the HOM waveguides and their intersection with the cavity wall on the local surface heating is also an important factor, since it determines the highest stresses in the cavity body. This was taken into account during the optimization so that the stresses could be reduced at the same time as the HOP damping was improved over previous designs. A new method of calculating the RF heating was employed, using a recently released high frequency electromagnetic element in ANSYS. This greatly facilitates the thermal and stress analysis of the design and fabrication methods have been developed with the goals of lower stresses, fewer parts and simpler assembly compared to previous designs. This should result in substantial cost savings. Preliminary designs are described for the cavity ancillary components including the RF window, HOM loads, and tuners. A preliminary manufacturing plan is included, with an initial estimate of the resource requirements. Other cavity options are discussed which might be desirable to either lower the R/Q, for reduced transient response, or lower the residual HOM impedance to reduce coupled-bunch growth rates further still.

  4. Thermoelastic damping effect of the micro-ring resonator with irregular mass and stiffness

    NASA Astrophysics Data System (ADS)

    Kim, Jung-Hwan; Kim, Ji-Hwan

    2016-05-01

    Fundamentally, vibration characteristic is a main factor for the stability of structures. In this regard, the irregularity of mass and stiffness distributions for the structure have been an interesting issue for many years. Recently, the Micro Electro Mechanical Systems (MEMS) are developed for various applications such as gyro sensors. In the present work, in-plane vibration of micro-ring structure with multiple finite-sized imperfections is investigated. Then, the unbalance of the structure is represented using Heaviside Step Function for the inextensional modeling of the ring. Also, thermoelastic damping (TED) due to internal friction is studied based on Fourier's one-dimensional heat conduction equation using Laplace Transform. To obtain the quality-factors (Q-factors) for imperfect micro-ring, analytical solutions are calculated from governing equations of motion with TED. And then, the natural frequencies and the Q-factors are observed to separate into lower and higher modes. Additionally, the vibration mode shapes are presented, and the frequency trimming concept due to attached imperfections is investigated.

  5. Longitudinal Single-Bunch Instability in the ILC Damping Rings: Estimate of Current Threshold

    SciTech Connect

    Venturini, Marco; Venturini, Marco

    2008-06-25

    Characterization of single-bunch instabilities in the International Linear Collider (ILC) damping rings (DRs) has been indicated as a high-priority activity toward completion of an engineering design. In this paper we report on a first estimate ofthe current thresholds for the instability using numerical and analytical models of the wake potentials associated with the various machine components. The numerical models were derived (upon appropriate scaling) from designs of the correspondingcomponents installed in existing machines. The current thresholds for instabilities were determined by numerical solution of the Vlasov equation for the longitudinal dynamics. For the DR baseline lattice as of Feb. 2007 we find the critical current forinstability to be safely above the design specifications leaving room for further optimization of the choice of the momentum compaction.

  6. HOM damping with coaxial dampers in the storage ring cavities of the Advanced Photon Source

    SciTech Connect

    Kang, Y.W.; Kustom, R.L.

    1994-08-01

    Coaxial dampers with E-probe and H-loop couplers are used to damp higher-order modes (HOM) in a 352-MHz single cell cavity for the storage ring of the Advanced Photon Source (APS). Measurements have been made with three different types of dampers such as E-probe dampers, small H-loop dampers, and H-loop dampers with {lambda}/4 short stub. Two dampers are used in each type. The dampers without fundamental frequency rejection filters are positioned to have a minimum deQing at the fundamental frequency: the E-probe dampers are used at the equatorial plane of the cavity, and the small H-loop dampers are used in the end wall of the cavity. The fundamental mode decoupling can be done by positioning the loop plane in the direction of the H-field of the mode.

  7. Particle-in-Cell Calculationsof the Electron Cloud in the ILCPositron Damping Ring Wigglers

    SciTech Connect

    Celata, C.M.; Furman, M.A.; Vay, J.-L.; Grote, D.P.

    2007-07-01

    The self-consistent code suite WARP-POSINST is being used to study electron cloud effects in the ILC positron damping ring wiggler. WARP is a parallelized, 3D particle-in-cell code which is fully self-consistent for all species. The POSINST models for the production of photoelectrons and secondary electrons are used to calculate electron creation. Mesh refinement and a moving reference frame for the calculation will be used to reduce the computer time needed by several orders of magnitude. We present preliminary results for cloud buildup showing 3D electron effects at the nulls of the vertical wiggler field. First results from a benchmark of WARP-POSINST vs. POSINST are also discussed.

  8. DC septum magnets for the damping rings of the SLC SLAC Linear Collider

    SciTech Connect

    Bijleveld, J.; Peterson, J.M.; Jensen, D.

    1986-07-01

    The injection/extraction systems of the 1.21 GeV Stanford Linear Collider (SLC) damping rings uses four pairs of water cooled septum magnets. Each pair consists of a thin-septum, low-field (3 mm, 3 kilogauss) magnet plus a thick-septum, high-field (12 mm, 8 kilogauss) model. In the latest design cooling reliability was improved by using stainless-steel tubing imbedded in the copper. The operating current in each is 2600 amperes, at a density of up to 120 amperes per mmS. Plasma-sprayed alumina is used to provide electrical insulation. The magnet system is compatible with 10 Z torr ultra-high vacuum. The magnet design, fabrication, and measurements are described.

  9. Beam Ion Instability in ILC Damping Ring with Multi-Gas Species

    SciTech Connect

    Wang, Lanfa; Pivi, Mauro; /SLAC

    2012-05-30

    Ion induced beam instability is one critical issue for the electron damping ring of the International Linear Collider (ILC) due to its ultra small emittance of 2 pm. The beam ion instability with various beam filling patterns for the latest lattice DTC02 is studied using PIC code. The code has been benchmarked with SPEAR3 experimental data and there is a good agreement between the simulation and observations. It uses the optics from MAD and can handle arbitrary beam filling pattern and vacuum. Different from previous studies, multi-gas species and exact beam filling patterns have been modeled simultaneously in the study. This feature makes the study more realistic. Analyses have been done to compare with the simulations.

  10. CESR Conversion Damping Ring Studies of Electron Cloud Instabilities (CESR-TA)

    SciTech Connect

    Rubin, David L.; Palmer, Mark A.

    2011-08-02

    In the International Linear Collider, two linear accelerators will accelerate bunches of positrons and electrons to over a hundred billion electron volts and collide them in a central detector. In order to obtain useful collision rates, the bunches, each containing twenty billion particles, must be compressed to a cross section of a few nanometers by a few hundred nanometers. In order to prepare these ultra high density bunches, damping rings (DRs) are employed before the linear accelerators. The DRs take the high emittance bunches that are provided by the electron and positron sources and, through the process of radiation damping, squeeze them into ultra low emittance beams that are ready for the main linear accelerators. In the damping rings, a number of effects can prevent the successful preparation of the beams. In the electron ring, an effect known as the fast ion instability can lead to beam growth and, in the positron ring, the build-up of an electron cloud (EC), which interacts with the circulating bunches, can produce the same effect. EC build-up and the subsequent interaction of the cloud with the positron beam in the DR have been identified as major risks for the successful construction of a linear collider. The CESRTA research program at the Cornell Electron Storage Ring (CESR) was developed in order to study the build-up of the EC, the details of its impact on ultra low emittance beams, as well as methods to mitigate the impact of the cloud. In the DR, the EC forms when synchrotron photons radiated from the circulating beam strike the walls of the vacuum chamber, resulting in the emission of photoelectrons. These low energy electrons can be accelerated across the vacuum chamber by the electric field of the beam, and strike the walls, causing the emission of secondary electrons. The secondary electrons are subsequently accelerated into the walls yet again via the same mechanism. The result is that the EC can rapidly begin to fill the vacuum chamber. In

  11. Phase detector and phase feedback for a single bunch in a two-bunch damping ring for the SLAC Linear Collider

    SciTech Connect

    Schwarz, H.D.; Judkins, J.G.

    1987-03-01

    The synchronous phase of a bunch of positrons or electrons being damped in a SLAC Linear Collider (SLC) damping ring is dependent on beam intensity. Injection for alternate bunches into the SLC linac from the damping rings should occur at a constant phase. A phase detector was developed allowing the measurement of phase of a single-stored bunch in the presence of a second bunch in reference to the phase of the linac. The single-bunch phase is derived from beam position monitor signals using a switching scheme to separate the two bunches circulating in each damping ring. The hardware is described including feedback loops to stabilize the extraction phase.

  12. Energy matching of 1. 2 GeV positron beam to the SLC (Stanford Linear Collider) damping ring

    SciTech Connect

    Clendenin, J.E.; Helm, R.H.; Jobe, R.K.; Kulikov, A.; Sheppard, J.C.

    1989-08-01

    Positrons collected at the SLC positron source are transported over a 2-km path at 220 MeV to be reinjected into the linac for acceleration to 1.2 GeV, the energy of the emittance damping ring. Since the positron bunch length is a significant fraction of a cycle of the linac-accelerating RF, the energy spread at 1.2 GeV is considerably larger than the acceptance of the linac-to-ring (LTR) transport system. Making use of the large pathlength difference at the beginning of the LTR due to this energy spread, a standard SLAC 3-m accelerating section has been installed in the LTR to match the longitudinal phase space of the positron beam to the acceptance of the damping ring. The design of the matching system is described, and a comparison of operating results within simulations is presented. 5 refs., 4 figs., 1 tab.

  13. ILC Damping Rings: Benefit of the Antechamber or: Antechamber vs. SEY

    SciTech Connect

    Furman, M. A.

    2011-03-30

    We present simulation results of the build-up of the electron-cloud density ne for the two proposed ILC damping ring lattices, DC04 and DSB3, with particular attention to the potential benefit of an antechamber. We examine a field-free region and a dipole bending magnet, with or without an antechamber. We assume a secondary electronemission model for the chamber surface based on approximate fits to measured data for TiN, except that we let the peak value of the secondary emission yield (SEY), delta max, be a variable. We conclude that there is a critical value of delta max below which the antechamber provides a substantial benefit, roughly a factor ~;;40 reduction in ne relative to the case in which max exceeds the critical value. We estimate the steady-state value of ne as a function of delta max, and thereby obtain the critical value of delta max for all cases considered. Thus, from the perspective of the electron-cloud effect, the inclusion of an antechamber in the design is justified only if delta max is below the critical value. The results presented here constitute a slight extension of those previously presented in March and September, 2010 [1, 2].

  14. Effect of Wiggler insertions on the single-particle dynamics of the NLC main damping rings

    SciTech Connect

    Venturini, Marco

    2003-07-30

    As they are expected to occupy a large portion of the lattice, wiggler insert ions will introduce significant linear and nonlinear perturbations to the single-particle dynamics in the NLC Main Damping Rings (MDR). The nonlinearities are of particular concern as a sufficiently large Dynamic Aperture (DA) is required for high injection efficiency. The main content of this report is a study of the wigglers impact on the DA of the NLC-MDR latest lattice design. The particle dynamics is modeled by transfer maps calculated by integration through the wiggler fields. For field representation we employed a 3D multipole expansion derived from the field data that were obtained with the aid of a magnet design code. Additional contents of this paper include an investigation of a simplified model of wiggler consisting of a sequence of standard magnet elements (where thin octupoles are used to represent the dominant nonlinearities) and the suggestion of a possible correction scheme to compensate the wiggler nonlinearities.

  15. Multi Optical Transition Radiation System for ATF2

    SciTech Connect

    Alabau-Gonzalvo, Javier; Blanch Gutierrez, Cesar; Civera, Jose Vicente; Faus-Golfe, Angeles; Garcia-Garrigos, Juan; Cruz, Juan; McCormick, Douglas; White, Glen; /SLAC

    2012-07-13

    In this paper we describe the design, installation and first calibration tests of a Multi Optical Transition Radiation System in the beam diagnostic section of the Extraction (EXT) line of ATF2, close to the multi wire scanner system. This system will be a valuable tool for measuring beam sizes and emittances coming from the ATF Damping Ring. With an optical resolution of about 2 {micro}m an original OTR design (OTR1X) located after the septum at the entrance of the EXT line demonstrated the ability to measure a 5.5 {micro}m beam size in one beam pulse and to take many fast measurements. This gives the OTR the ability to measure the beam emittance with high statistics, giving a low error and a good understanding of emittance jitter. Furthermore the nearby wire scanners will be a definitive test of the OTR as a beam emittance diagnostic device. The multi-OTR system design proposed here is based on the existing OTR1X.

  16. Recent electron-cloud simulation results for the main damping rings of the NLC and TESLA linear colliders

    SciTech Connect

    Pivi, M.; Raubenheimer, T.O.; Furman, M.A.

    2003-05-01

    In the beam pipe of the Main Damping Ring (MDR) of the Next Linear Collider (NLC), ionization of residual gases and secondary emission give rise to an electron-cloud which stabilizes to equilibrium after few bunch trains. In this paper, we present recent computer simulation results for the main features of the electron cloud at the NLC and preliminary simulation results for the TESLA main damping rings, obtained with the code POSINST that has been developed at LBNL, and lately in collaboration with SLAC, over the past 7 years. Possible remedies to mitigate the effect are also discussed. We have recently included the possibility to simulate different magnetic field configurations in our code including solenoid, quadrupole, sextupole and wiggler.

  17. ATF2 Proposal

    SciTech Connect

    Grishanov, Boris Ivanovich; Logachev, Pavel; Podgorny, Fedor; Telnov, Valery; Angal-Kalinin, Deepa; Appleby, Robert; Jones, James; Kalinin, Alexander; Napoly, Olivier; Payet, Jacques; Braun, Hans-Heinrich; Schulte, Daniel; Zimmermann, Frank; Barlow, Roger; Bailey, Ian; Jenner, Leo; Jones, Roger; Kourevlev, German; Walker, Nick; Takahasi, Tohru; Gao, Jie; /Beijing, Inst. High Energy Phys. /Oxford U. /KEK, Tsukuba /Kyoto U., Inst. Chem. Res. /Orsay, LAL /LBL, Berkeley /LLNL, Livermore /University Coll. London /Chiba, Natl. Inst. Rad. Sci. /North Carolina A-T State U. /Oregon U. /Pohang Accelerator Lab. /Queen Mary, U. of London /SLAC /Tokyo U.

    2005-08-23

    This document is the first of two volumes describing the ATF2 project. The present volume discusses the technical justification for ATF2 and presents a design description. Since the International Committee for Future Accelerator (ICFA) decision on the choice of technology, a world-wide collaboration on the design of the International Linear Collider (ILC) has rapidly progressed [1]. The formation of the Global Design Effort (GDE) will accelerate the work towards a final design. An important technical challenge is obviously the high gradient acceleration but what is similarly challenging is the collision of extremely small beams of a few nanometer size. The latter challenge has three distinct issues: creating small emittance beams, preserving the emittance during acceleration and transport, and focusing the beams to nanometers. Most studies have been done using computer simulations but many issues still remain that require experimental verification. Accelerator Test Facility (ATF) at KEK was built to create small emittance beams, and succeeded in obtaining an emittance that almost satisfies the ILC requirements [2]. In this proposal we present a project, ATF2, which addresses the focusing of the beam into a nanometer spot. The ATF2 project will extend the extraction beamline of the ATF with an ILC-type final focus system to create a tightly focused, stable beam by making use of the small emittance of the ATF. The layout is shown in Figure 1.1. In the longer term, the ATF2 project would also provide invaluable input for the CLIC design of a future multi-TeV collider.

  18. Beam-Based Alignment, Tuning and Beam Dynamics Studies for the ATF2 Extraction Line and Final Focus System

    SciTech Connect

    White, Glen R.; Molloy, S.; Woodley, M.; /SLAC

    2008-07-25

    Using a new extraction line currently under construction, the ATF2 experiment plans to test the novel compact final focus optics design with local chromaticity correction intended for use in future linear colliders. With a 1.3 GeV design beam of 30nm normalized vertical emittance extracted from the ATF damping ring, the primary goal is to achieve a vertical spot-size at the IP waist of 37nm. We discuss our planned strategy for tuning the ATF2 beam to meet the primary goal. Simulation studies have been performed to asses the effectiveness of the strategy, including 'static' (installation) errors and dynamical effects (ground-motion, mechanical vibration, ring extraction jitter etc.). We have simulated all steps in the tuning procedure, from initial orbit establishment to final IP spot-size tuning. Through a Monte Carlo study of 100's of simulation seeds we find we can achieve a spot-size within {approx}10% of the design optics value in at least 75% of cases. We also ran a simulation to study the long-term performance with the use of beam-based feedbacks.

  19. RF cavity R&D at LBNL for the NLC Damping Rings,FY2000/2001

    SciTech Connect

    Rimmer, R.A.; Atkinson, D.; Corlett, J.N.; Koehler, G.; Li, D.; Hartman, N.; Rasson, J.; Saleh, T.; Weidenbach, W.

    2001-06-01

    This report contains a summary of the R&D activities at LBNL on RF cavities for the NLC damping rings during fiscal years 2000/2001. This work is a continuation of the NLC RF system R&D of the previous year [1]. These activities include the further optimization and fine tuning of the RF cavity design for both efficiency and damping of higher-order modes (HOMs). The cavity wall surface heating and stresses were reduced at the same time as the HOM damping was improved over previous designs. Final frequency tuning was performed using the high frequency electromagnetic analysis capability in ANSYS. The mechanical design and fabrication methods have been developed with the goals of lower stresses, fewer parts and simpler assembly compared to previous designs. This should result in substantial cost savings. The cavity ancillary components including the RF window, coupling box, HOM loads, and tuners have been studied in more detail. Other cavity options are discussed which might be desirable to either further lower the HOM impedance or increase the stored energy for reduced transient response. Superconducting designs and the use of external ''energy storage'' cavities are discussed. A section is included in which the calculation method is summarized and its accuracy assessed by comparisons with the laboratory measurements of the PEP-II cavity, including errors, and with the beam-sampled spectrum.

  20. Novel Slide-Ring Material/Natural Rubber Composites with High Damping Property

    NASA Astrophysics Data System (ADS)

    Wang, Wencai; Zhao, Detao; Yang, Jingna; Nishi, Toshio; Ito, Kohzo; Zhao, Xiuying; Zhang, Liqun

    2016-03-01

    A novel class of polymers called “slide-ring” (SR) materials with slideable junctions were used for high damping composites for the first time. The SR acts as the high damping phase dispersed in the natural rubber (NR) matrix, and epoxidized natural rubber (ENR) acts as the compatibilizer. The morphological, structural, and mechanical properties of the composites were investigated by atomic force microscope (AFM), transmission electron microscope (TEM), dynamic mechanical thermal analyzer (DMTA), rubber processing analyzer (RPA), and tensile tester. AFM and TEM results showed that the SR phase was uniformly dispersed in the composites, in a small size that is a function of ENR. DMTA and RPA results showed that the damping factor of the composites is much higher than that of NR, especially at room temperatures. Stretch hysteresis was used to study the energy dissipation of the composites at large strains. The results showed that SR and ENR can significantly improve the dissipation efficiency at strains lower than 200% strain. Wide-angle X-ray diffraction was used to study the strain-induced crystallization of the composites. The results indicated that the impact of the SR on the crystallization of NR is mitigated by the insulating effect of ENR.

  1. Novel Slide-Ring Material/Natural Rubber Composites with High Damping Property

    PubMed Central

    Wang, Wencai; Zhao, Detao; Yang, Jingna; Nishi, Toshio; Ito, Kohzo; Zhao, Xiuying; Zhang, Liqun

    2016-01-01

    A novel class of polymers called “slide-ring” (SR) materials with slideable junctions were used for high damping composites for the first time. The SR acts as the high damping phase dispersed in the natural rubber (NR) matrix, and epoxidized natural rubber (ENR) acts as the compatibilizer. The morphological, structural, and mechanical properties of the composites were investigated by atomic force microscope (AFM), transmission electron microscope (TEM), dynamic mechanical thermal analyzer (DMTA), rubber processing analyzer (RPA), and tensile tester. AFM and TEM results showed that the SR phase was uniformly dispersed in the composites, in a small size that is a function of ENR. DMTA and RPA results showed that the damping factor of the composites is much higher than that of NR, especially at room temperatures. Stretch hysteresis was used to study the energy dissipation of the composites at large strains. The results showed that SR and ENR can significantly improve the dissipation efficiency at strains lower than 200% strain. Wide-angle X-ray diffraction was used to study the strain-induced crystallization of the composites. The results indicated that the impact of the SR on the crystallization of NR is mitigated by the insulating effect of ENR. PMID:26949077

  2. Computer studies of a combined-function bend magnet for a proposed redesign of the SLAC SLC damping rings

    SciTech Connect

    Early, R.A.; Raubenheimer, T.O.

    1993-04-01

    A proposed redesign of the SLAC SLC Damping Ring requires a combined-function bending magnet. The magnet will operate with a main field of 1.8338 T, and quadrupole and sextupole gradients dB{sub y}/dx, d{sup 2}B{sub y}/dx{sup 2} of {minus}14.1 T/m and {minus}477 T/m{sup 2}, respectively. Because the orbit sagitta in the magnet is in excess of 2 cm, the pole will be curved with a 2 m radius of curvature. Furthermore, since the current must be variable over a range of {plus_minus}2 percent, we have considered using vanadium permendur poles to avoid a adverse saturation effects. Studies were done using POISSON in 2-D and TOSCA for 3-D end effects.

  3. Simulation of radiation damping in rings, using stepwise ray-tracing methods

    NASA Astrophysics Data System (ADS)

    Méot, F.

    2015-06-01

    The ray-tracing code Zgoubi computes particle trajectories in arbitrary magnetic and/or electric field maps or analytical field models. It includes a built-in fitting procedure, spin tracking, many Monte Carlo processes. The accuracy of the integration method makes it an efficient tool for multi-turn tracking in periodic machines. Energy loss by synchrotron radiation, based on Monte Carlo techniques, had been introduced in Zgoubi in the early 2000s for studies regarding the linear collider beam delivery system. However, only recently has this Monte Carlo tool been used for systematic beam dynamics and spin diffusion studies in rings, including the eRHIC electron-ion collider project at the Brookhaven National Laboratory. Some beam dynamics aspects of this recent use of Zgoubi capabilities, including considerations of accuracy as well as further benchmarking in the presence of synchrotron radiation in rings, are reported here.

  4. Simulation of radiation damping in rings, using stepwise ray-tracing methods

    DOE PAGESBeta

    Meot, F.

    2015-06-26

    The ray-tracing code Zgoubi computes particle trajectories in arbitrary magnetic and/or electric field maps or analytical field models. It includes a built-in fitting procedure, spin tracking many Monte Carlo processes. The accuracy of the integration method makes it an efficient tool for multi-turn tracking in periodic machines. Energy loss by synchrotron radiation, based on Monte Carlo techniques, had been introduced in Zgoubi in the early 2000s for studies regarding the linear collider beam delivery system. However, only recently has this Monte Carlo tool been used for systematic beam dynamics and spin diffusion studies in rings, including eRHIC electron-ion collider projectmore » at the Brookhaven National Laboratory. Some beam dynamics aspects of this recent use of Zgoubi capabilities, including considerations of accuracy as well as further benchmarking in the presence of synchrotron radiation in rings, are reported here.« less

  5. Simulation of radiation damping in rings, using stepwise ray-tracing methods

    SciTech Connect

    Meot, F.

    2015-06-26

    The ray-tracing code Zgoubi computes particle trajectories in arbitrary magnetic and/or electric field maps or analytical field models. It includes a built-in fitting procedure, spin tracking many Monte Carlo processes. The accuracy of the integration method makes it an efficient tool for multi-turn tracking in periodic machines. Energy loss by synchrotron radiation, based on Monte Carlo techniques, had been introduced in Zgoubi in the early 2000s for studies regarding the linear collider beam delivery system. However, only recently has this Monte Carlo tool been used for systematic beam dynamics and spin diffusion studies in rings, including eRHIC electron-ion collider project at the Brookhaven National Laboratory. Some beam dynamics aspects of this recent use of Zgoubi capabilities, including considerations of accuracy as well as further benchmarking in the presence of synchrotron radiation in rings, are reported here.

  6. High brightness, high current injector design for the ATF upgrade at Brookhaven National Laboratory

    NASA Astrophysics Data System (ADS)

    Stratakis, Diktys

    2015-04-01

    Brookhaven National Accelerator Test Facility (BNL ATF) is in the process of moving to a new place and upgrading its major capabilities: The electron beam energy and CO2 laser power. Specifically, the maximum electron beam energy will be first projected to 100-150 MeV and then upgraded to 500 MeV while at the same time the laser power will increase 100 fold, thus making the new ATF a powerful tool in advanced accelerator concept research. The bright electron bunch produced by the new state-of-the-art photocathode rf gun will be accelerated and optionally delivered to multiple beamlines. The injector is a key element of this accelerator upgrade. It must deliver a high average current beam with very small transverse and longitudinal emittances, at a sufficiently high energy that space charge effects are under control. We review here the detailed injector design and present first results from beam dynamics simulations. We give emphasis in the production of compressed flat beams which have important applications in novel light-source concepts and could possibly alleviate the need for damping rings in lepton colliders. We present a theoretical model and with the aid of simulation examine the influence of space charge, bunch compression and suggest a operating regime with minimal phase space dilutions.

  7. ATF2 Proposal Volume 2

    SciTech Connect

    Grishanov, B.I.; Logachev, P.; Podgorny, F.; Telnov, V.; Angal-Kalinin, D.; Jones, J.; Kalinin, A.; Napoly, O.; Payet, J.; Braun, H.H.; Schulte, D.; Zimmermann, F.; Appleby, R.; Barlow, R.; Bailey, I.; Jenner, L.; Jones, R.; Kourevlev, G.; Elsen, E.; Vogel, V.; Walker, N.; /DESY /Fermilab /Hiroshima U. /Beijing, Inst. High Energy Phys. /Oxford U. /Royal Holloway, U. of London /KEK, Tsukuba /Kyoto U., Inst. Chem. Res. /Orsay, LAL /Valencia U. /Annecy, LAPP /LBL, Berkeley /LLNL, Livermore /University Coll. London /Chiba, Natl. Inst. Rad. Sci. /North Carolina A-T State U. /Oregon U. /Pohang Accelerator Lab. /Queen Mary, U. of London /SLAC /Tokyo U.

    2006-02-27

    For achieving the high luminosity required at the International Linear Collider (ILC), it is critical to focus the beams to nanometer size with the ILC Beam Delivery System (BDS), and to maintain the beam collision with a nanometer-scale stability. To establish the technologies associated with this ultra-high precision beam handling, it has been proposed to implement an ILC-like final focus optics in an extension of the existing extraction beamline of ATF at KEK. The ATF is considered to be the best platform for this exercise, since it provides an adequate ultra-low emittance electron beam in a manner dedicated to the development of ILC. The two major goals for this facility, called ATF2, are: (A) Achievement of a 37 nm beam size, and (B) control of beam position down to 2 nm level. The scientific justification for the ATF2 project and its technical design have been described in Volume 1 of the ATF2 Proposal [1]. We present here Volume 2 of the ATF2 Proposal, in which we present specifics of the construction plans and the group organization to execute the research programs at ATF2. The sections in this report have been authored by relevant ATF2 subgroups within the International ATF Collaboration. The time line of the project is described in Section 2. Section 3 discuss the structure of the international collaboration. Sections 4 and 5 discuss budget considerations, which are presented as well as the design and construction tasks to be shared by the international collaboration at ATF2. Concluding remarks have been contributed by Dr. Ewan Paterson, Chair of the International Collaboration Board of the ATF collaboration.

  8. Bolometric measurements in ATF

    SciTech Connect

    Hiroe, S.; Wilgen, J.B.; Batchelor, D.B.; Bigelow, T.S.; Wing, W.R.; Bell, G.L.; Gandy, R.F.; Goldfinger, R.C.; Kindsfather, R.R.

    1989-01-01

    Bolometer measurements are made under various plasma conditions in ATF. Each bolometer module includes four pairs of detectors; each pair consists of a sensor and a reference detector. Two or three detector pairs in each module were installed with various types of aperture masks to serve as radiation detectors. The rest were completely shielded from plasma emissions and used as blind detectors to measure background levels. When electron cyclotron heating (ECH) power was applied to ATF, the radiation signal was more than 10 times higher than that expected for 100 kW of radiated power. Surprisingly, similar signal levels were observed on the blind detectors. It is hypothesized that the bolometers are sensitive to ECH power at 53 GHz. (This problem may be general in near-term fusion experiments, in which microwave power will be an important tool for plasma heating.) In this paper, experiments aimed at proving this hypothesis are reported; bolometric signals before and after chromium gettering are discussed; and measurements of the microwave power deposition profile around the torus, made by using the observed microwave interference with the blind detectors, are presented. 4 refs., 4 figs.

  9. STREAK damping. Technical report

    SciTech Connect

    Baker, J.; Peyton, S.; Freiberg, H.

    1989-12-01

    This report documents a study aimed at improving the damping in STREAK. A form and value for an artificial viscosity is recommended which appears to control ringing and overshoots without overdamping.

  10. BNL ATF II beamlines design

    SciTech Connect

    Fedurin, M.; Jing, Y.; Stratakis, D.; Swinson, C.

    2015-05-03

    The Brookhaven National Laboratory. Accelerator Test Facility (BNL ATF) is currently undergoing a major upgrade (ATF-II). Together with a new location and much improved facilities, the ATF will see an upgrade in its major capabilities: electron beam energy and quality and CO2 laser power. The electron beam energy will be increased in stages, first to 100-150 MeV followed by a further increase to 500 MeV. Combined with the planned increase in CO2 laser power (from 1-100 TW), the ATF-II will be a powerful tool for Advanced Accelerator research. A high-brightness electron beam, produced by a photocathode gun, will be accelerated and optionally delivered to multiple beamlines. Besides the energy range (up to a possible 500 MeV in the final stage) the electron beam can be tailored to each experiment with options such as: small transverse beam size (<10 um), short bunch length (<100 fsec) and, combined short and small bunch options. This report gives a detailed overview of the ATFII capabilities and beamlines configuration.

  11. Status of the ATF2 Lattices

    SciTech Connect

    Marin, E.; Tomas, R.; Bambade, P.; Okugi, T.; Tauchi, T.; Terunuma, N.; Urakawa, J.; Seryi, A.; White, G.; Woodley, M.; /SLAC

    2011-12-09

    The current status for the ATF2 Nominal and Ultra-low {beta}* lattices are presented in this paper. New lattice designs have been obtained in order to minimise the impact of the last interpretation of multipole measurements that have been included into the model. However, the new ATF2 Ultra-low design is not able to recover the expected vertical beam size at the IP with the current magnet distribution. Therefore, different quadrupole sorting have been studied. A significant gain is evident for the ATF2 Ultra-low lattice when sorting the magnets according to the skew-sextupolar components. The ATF2 Nominal lattice is also expected to benefit from the new sorting. Tuning results of the new ATF2 Ultra-low lattice under realistic imperfections are also reported.

  12. Analysis of KEK-ATF Optics and Coupling Using LOCO

    SciTech Connect

    Wolski, Andrzej; Ross, Marc; Woodley, Mark; Nelson, Janice; Mishra, Shekhar

    2004-01-12

    LOCO is a code for analysis of the linear optics in astorage ring based on the closed orbit response to steering magnets. Theanalysis provides information on focusing errors, BPM gain and rotationerrors,and local coupling. Here, we report the results of an applicationof LOCO to the KEK-ATF. Although the analysis appears to have provideduseful information on the optics of the machine, it appears that one ofthe main aims of the study to reduce the vertical emittance by correctingthe local coupling was not successful, and we discuss some possiblereasons for this.

  13. Rings

    SciTech Connect

    Davis, R.L.

    1989-01-01

    The essence of vortex physics is that at certain low-energy scales elementary excitations of a point particle theory can behave like strings rather than particles. Vortices are the resulting string-like solutions; their thickness sets the distance scale beyond which physics is string-like rather than particle-like. String degrees of freedom are massless in the sense that excitations on a string can have an arbitrarily low frequency. Non-string degrees of freedom correspond to massive particles and are absent from the low energy spectrum. This article considers only field theories with vortices at low energies. The possible existence of a class of solitons in these vortex theories will be discussed. They are vortex rings: they are localized and finite in energy, and able to carry the quantum numbers of point particles. Rings are thus particle-like solutions of a vortex theory, which is itself a limit of a point particle field theory.

  14. ATF (Advanced Toroidal Facility) data management

    SciTech Connect

    Kannan, K.L.; Baylor, L.R.

    1988-01-01

    Data management for the Advanced Toroidal Facility (ATF), a stellarator located at Oak Ridge National Laboratory (ORNL), is provided by DMG, a locally developed, VAX-based software system. DMG is a data storage and retrieval software system that provides the user interface to ATF raw and analyzed data. Data are described in terms of data models and data types and are organized as signals into files, which are internally documented. The system was designed with user accessibility, software maintainability, and extensibility as primary goals. Extensibility features include compatibility with ATF as it moves from pulsed to steady-state operation and capability for use of the DMG system with experiments other than ATF. DMG is implemented as a run-time library of routines available as a shareable image. General-purpose and specialized data acquisition and analysis applications have been developed using the DMG system. This paper describes the DMG system and the interfaces to it. 4 refs., 2 figs.

  15. The Straightness Monitor System at ATF2

    SciTech Connect

    Hildreth, Michael; Aryshev, Alexander; Boogert, Stewart; Honda, Yosuke; Tauchi, Toshiaki; Terunuma, Nobuhiro; White, Glen; /SLAC

    2012-07-06

    The demonstration of absolute stability of the position of the focused beam is the primary goal of the ATF2 commissioning effort. We have installed a laser interferometer system that will eventually correct the measurement of high-precision Beam Position Monitors used in the ATF2Final Focus Steering Feedback for mechanical motion or vibrations. Here, we describe the installed system and present preliminary data on the short- and long-term mechanical stability of the BPM system.

  16. Damped flexible seal

    DOEpatents

    DuBois, Neil J.; Amaral, Antonio M.

    1992-10-27

    A damped flexible seal assembly for a torpedo isolates the tailcone thereof rom vibrational energy present in the drive shaft assembly. A pair of outside flanges, each of which include an inwardly facing groove and an O-ring constrained therein, provide a watertight seal against the outer non-rotating surface of the drive shaft assembly. An inside flange includes an outwardly-facing groove and an O-ring constrained therein, and provides a watertight seal against the inner surface of the tail cone. Two cast-in-place elastomeric seals provide a watertight seal between the flanges and further provide a damping barrier between the outside flanges and the inside flanges for damping vibrational energy present in the drive shaft assembly before the energy can reach the tailcone through the seal assembly.

  17. Expression Levels of the Yeast Alcohol Acetyltransferase Genes ATF1, Lg-ATF1, and ATF2 Control the Formation of a Broad Range of Volatile Esters

    PubMed Central

    Verstrepen, Kevin J.; Van Laere, Stijn D. M.; Vanderhaegen, Bart M. P.; Derdelinckx, Guy; Dufour, Jean-Pierre; Pretorius, Isak S.; Winderickx, Joris; Thevelein, Johan M.; Delvaux, Freddy R.

    2003-01-01

    Volatile aroma-active esters are responsible for the fruity character of fermented alcoholic beverages such as beer and wine. Esters are produced by fermenting yeast cells in an enzyme-catalyzed intracellular reaction. In order to investigate and compare the roles of the known Saccharomyces cerevisiae alcohol acetyltransferases, Atf1p, Atf2p and Lg-Atf1p, in volatile ester production, the respective genes were either deleted or overexpressed in a laboratory strain and a commercial brewing strain. Subsequently, the ester formation of the transformants was monitored by headspace gas chromatography and gas chromatography combined with mass spectroscopy (GC-MS). Analysis of the fermentation products confirmed that the expression levels of ATF1 and ATF2 greatly affect the production of ethyl acetate and isoamyl acetate. GC-MS analysis revealed that Atf1p and Atf2p are also responsible for the formation of a broad range of less volatile esters, such as propyl acetate, isobutyl acetate, pentyl acetate, hexyl acetate, heptyl acetate, octyl acetate, and phenyl ethyl acetate. With respect to the esters analyzed in this study, Atf2p seemed to play only a minor role compared to Atf1p. The atfatf2Δ double deletion strain did not form any isoamyl acetate, showing that together, Atf1p and Atf2p are responsible for the total cellular isoamyl alcohol acetyltransferase activity. However, the double deletion strain still produced considerable amounts of certain other esters, such as ethyl acetate (50% of the wild-type strain), propyl acetate (50%), and isobutyl acetate (40%), which provides evidence for the existence of additional, as-yet-unknown ester synthases in the yeast proteome. Interestingly, overexpression of different alleles of ATF1 and ATF2 led to different ester production rates, indicating that differences in the aroma profiles of yeast strains may be partially due to mutations in their ATF genes. PMID:12957907

  18. Quadratic Damping

    ERIC Educational Resources Information Center

    Fay, Temple H.

    2012-01-01

    Quadratic friction involves a discontinuous damping term in equations of motion in order that the frictional force always opposes the direction of the motion. Perhaps for this reason this topic is usually omitted from beginning texts in differential equations and physics. However, quadratic damping is more realistic than viscous damping in many…

  19. Damping device for a stationary labyrinth seal

    NASA Technical Reports Server (NTRS)

    El-Aini, Yehia M. (Inventor); Mitchell, William S. (Inventor); Roberts, Lawrence P. (Inventor); Montgomery, Stuart K. (Inventor); Davis, Gary A. (Inventor)

    2010-01-01

    A stationary labyrinth seal system includes a seal housing having an annular cavity, a plurality of damping devices, and a retaining ring. The damping devices are positioned within the annular cavity and are maintained within the annular cavity by the retaining ring.

  20. ATF3 inhibits PPARγ-stimulated transactivation in adipocyte cells

    SciTech Connect

    Jang, Min-Kyung; Jung, Myeong Ho

    2015-01-02

    Highlights: • ATF3 inhibits PPARγ-stimulated transcriptional activation. • ATF3 interacts with PPARγ. • ATF3 suppresses p300-mediated transcriptional coactivation. • ATF3 decreases the binding of PPARγ and recruitment of p300 to PPRE. - Abstract: Previously, we reported that activating transcription factor 3 (ATF3) downregulates peroxisome proliferator activated receptor (PPARγ) gene expression and inhibits adipocyte differentiation in 3T3-L1 cells. Here, we investigated another role of ATF3 on the regulation of PPARγ activity. ATF3 inhibited PPARγ-stimulated transactivation of PPARγ responsive element (PPRE)-containing reporter or GAL4/PPARγ chimeric reporter. Thus, ATF3 effectively repressed rosiglitazone-stimulated expression of adipocyte fatty acid binding protein (aP2), PPARγ target gene, in 3T3-L1 cells. Coimmunoprecipitation and GST pulldown assay demonstrated that ATF3 interacted with PPARγ. Accordingly, ATF3 prevented PPARγ from binding to PPRE on the aP2 promoter. Furthermore, ATF3 suppressed p300-mediated transcriptional coactivation of PPRE-containing reporter. Chromatin immunoprecipitation assay showed that overexpression of ATF3 blocked both binding of PPARγ and recruitment of p300 to PPRE on aP2 promoter induced by rosiglitazone treatment in 3T3-L1 cells. Taken together, these results suggest that ATF3 interacts with PPARγ and represses PPARγ-mediated transactivation through suppression of p300-stimulated coactivation in 3T3-L1 cells, which may play a role in inhibition of adipocyte differentiation.

  1. ATF3 represses PPARγ expression and inhibits adipocyte differentiation

    SciTech Connect

    Jang, Min-Kyung; Jung, Myeong Ho

    2014-11-07

    Highlights: • ATF3 decrease the expression of PPARγ and its target gene in 3T3-L1 adipocytes. • ATF3 represses the promoter activity of PPARγ2 gene. • ATF/CRE (−1537/−1530) is critical for ATF3-mediated downregulation of PPARγ. • ATF3 binds to the promoter region containing the ATF/CRE. • ER stress inhibits adipocyte differentiation through downregulation of PPARγ by ATF3. - Abstract: Activating transcription factor 3 (ATF3) is a stress-adaptive transcription factor that mediates cellular stress response signaling. We previously reported that ATF3 represses CCAAT/enhancer binding protein α (C/EBPα) expression and inhibits 3T3-L1 adipocyte differentiation. In this study, we explored potential role of ATF3 in negatively regulating peroxisome proliferator activated receptor-γ (PPARγ). ATF3 decreased the expression of PPARγ and its target gene in 3T3-L1 adipocytes. ATF3 also repressed the activity of −2.6 Kb promoter of mouse PPARγ2. Overexpression of PPARγ significantly prevented the ATF3-mediated inhibition of 3T3-L1 differentiation. Transfection studies with 5′ deleted-reporters showed that ATF3 repressed the activity of −2037 bp promoter, whereas it did not affect the activity of −1458 bp promoter, suggesting that ATF3 responsive element is located between the −2037 and −1458. An electrophoretic mobility shift assay and chromatin immunoprecipitation assay demonstrated that ATF3 binds to ATF/CRE site (5′-TGACGTTT-3′) between −1537 and −1530. Mutation of the ATF/CRE site abrogated ATF3-mediated transrepression of the PPARγ2 promoter. Treatment with thapsigargin, endoplasmic reticulum (ER) stress inducer, increased ATF3 expression, whereas it decreased PPARγ expression. ATF3 knockdown significantly blocked the thapsigargin-mediated downregulation of PPARγ expression. Furthermore, overexpression of PPARγ prevented inhibition of 3T3-L1 differentiation by thapsigargin. Collectively, these results suggest that ATF3-mediated

  2. Inverse Cerenkov laser acceleration experiment at ATF

    NASA Astrophysics Data System (ADS)

    Wang, X. J.; Pogorelsky, I.; Fernow, R.; Kusche, K. P.; Liu, Y.; Kimura, W. D.; Kim, G. H.; Romea, R. D.; Steinhauer, L. C.

    Inverse Cerenkov laser acceleration was demonstrated using an axicon optical system at the Brookhaven Accelerator Test Facility (ATF). The ATF S-band linac and a high power 10.6 MICROMETERSCO2 laser were used for the experiment. Experimental arrangement and the laser and the electron beams synchronization are discussed. The electrons were accelerated more than 0.7 MeV for a 34 MW CO2 laser power. More than 3.7 MeV acceleration was measured with 0.7 GW CO2 laser power, which is more than 20 times of the previous ICA experiment. The experimental results are compared with computer program TRANSPORT simulations.

  3. Landau damping

    SciTech Connect

    Ng, K.Y.; /Fermilab

    2010-10-01

    Section 2.5.8 of the Handbook of Accelerator Physics and Engineering on Landau damping is rewritten. An solvable example is first given to demonstrate the interplay between Landau damping and decoherence. This example is an actual one when the beam oscillatory motion is driven by a wake force. The dispersion relation is derived and its implication on Landau damping is illustrated. The rest of the article touches on the Landau damping of transverse and longitudinal beam oscillations. The stability criteria are given for a bunched beam and the changes of the criteria when the beam is lengthened and becomes unbunched.

  4. Coulomb Damping

    ERIC Educational Resources Information Center

    Fay, Temple H.

    2012-01-01

    Viscous damping is commonly discussed in beginning differential equations and physics texts but dry friction or Coulomb friction is not despite dry friction being encountered in many physical applications. One reason for avoiding this topic is that the equations involve a jump discontinuity in the damping term. In this article, we adopt an energy…

  5. A Superconducting Magnet Upgrade of the ATF2 Final Focus

    SciTech Connect

    Parker, Brett; Anerella, Michael; Escallier, John; He, Ping; Jain, Animesh; Marone, Andrew; Wanderer, Peter; Wu, Kuo-Chen; Bambade, Philip; Bolzon, Benoit; Jeremie, Andrea; Coe, Paul; Urner, David Hauviller, Claude; Marin, Eduardo; Tomas, Rogelio; Zimmermann, Frank; Kimura, Nobuhiro; Kubo, Kiyoshi; Kume, Tatsuya Kuroda, Shigeru; /KEK, Tsukuba /KEK, Tsukuba /KEK, Tsukuba /KEK, Tsukuba /KEK, Tsukuba /KEK, Tsukuba /KEK, Tsukuba /KEK, Tsukuba /SLAC /SLAC /SLAC

    2012-07-05

    The ATF2 facility at KEK is a proving ground for linear collider technology with a well instrumented extracted beam line and Final Focus (FF). The primary ATF2 goal is to demonstrate the extreme beam demagnification and spot stability needed for a linear collider FF. But the ATF2 FF uses water cooled magnets and the ILC baseline has a superconducting (SC) FF. We plan to upgrade ATF2 and replace some of the warm FF magnets with SC FF magnets. The ATF2 SC magnets, like the ILC FF, will made via direct wind construction. ATF2 coil winding is in progress at BNL and warm magnetic measurements indicate we have achieved good field quality. Studies indicate that having ATF2 FF magnets with larger aperture and better field quality should allow reducing the ATF2 FF beta function for study of focusing regimes relevant to CLIC. The ATF2 magnet cryostat will have laser view ports for directly monitoring cold mass movement. We plan to make stability measurements at BNL and KEK to relate ATF2 FF magnet performance to that of a full length ILC QD0 R&D FF prototype under construction at BNL.

  6. A Superconducting Magnet Upgrade of the ATF2 Final Focus

    SciTech Connect

    Parker B.; Anerella M.; Escallier J.; He P.; Jain A.; Marone A.; Wanderer P.; Wu K.C.; Hauviller C.; Marin E.; Tomas R.; Zimmermann F.; Bolzon B.; Jeremie A.; Kimura N.; Kubo K.; Kume T.; Kuroda S.; Okugi T.; Tauchi T.; Terunuma N.; Tomaru T.; Tsuchiya K.; Urakawa J.; Yamamoto A.; Bambabe P.; Coe P.; Urner D.; Seryi A.; Spencer C.; White G.

    2010-05-23

    The ATF2 facility at KEK is a proving ground for linear collider technology with a well instrumented extracted beam line and Final Focus (FF). The primary ATF2 goal is to demonstrate the extreme beam demagnification and spot stability needed for a linear collider FF. But the ATF2 FF uses water cooled magnets and the ILC baseline has a superconducting (SC) FF. We plan to upgrade ATF2 and replace some of the warm FF magnets with SC FF magnets. The ATF2 SC magnets, like the ILC FF, will made via direct wind construction. ATF2 coil winding is in progress at BNL and warm magnetic measurements indicate we have achieved good field quality. Studies indicate that having ATF2 FF magnets with larger aperture and better field quality should allow reducing the ATF2 FF beta function for study of focusing regimes relevant to CLIC. The ATF2 magnet cryostat will have laser view ports for directly monitoring cold mass movement. We plan to make stability measurements at BNL and KEK to relate ATF2 FF magnet performance to that of a full length ILC QD0 R and D FF prototype under construction at BNL.

  7. Inverse Cerenkov laser acceleration experiment at ATF

    SciTech Connect

    Wang, X.J.; Pogorelsky, I.; Fernow, R.; Kusche, K.P.; Liu, Y.; Kimura, W.D.; Kim, G.H.; Romea, R.D.; Steinhauer, L.C.

    1994-09-01

    Inverse Cerenkov laser acceleration was demonstrated using an axicon optical system at the Brookhaven Accelerator Test Facility (ATF). The ATF S-band linac and a high power 10.6 {mu}m CO{sub 2} laser were used for the experiment. Experimental arrangement and the laser and the electron beams synchronization are discussed. The electrons were accelerated more than 0.7 MeV for a 34 MW CO{sub 2} laser power. More than 3.7 MeV acceleration was measured with 0.7 GW CO{sub 2} laser power, which is more than 20 times of the previous ICA experiment. The experimental results are compared with computer program TRANSPORT simulations.

  8. miRNA-1283 Regulates the PERK/ATF4 Pathway in Vascular Injury by Targeting ATF4

    PubMed Central

    Xu, Qingyun; Chen, Ruixue; Chen, Liguo

    2016-01-01

    Background In our previous study, we found significant differences in the mRNA and microRNA (miRNA) levels among hypertensive patients with different degrees of vascular endothelial cells damage. These differences were closely associated with endoplasmic reticulum stress (ERS)-related proteins. Moreover, compared to the control group, the expression of transcription factor activating factor 4 (ATF4) was also found to be significantly different in the hypertensive patients with different degrees of vascular endothelial cells damage groups. These results were confirmed using gene prediction software, which showed synergistic effects between ATF4 and miR-1283. ATF4 is a key molecule in ERS. Three ERS pathways exist:protein kinase RNA-like ER kinase (PERK), activating transcription factor 6 (ATF6) and inositol-requiring enzyme-1 (IRE-1)-induced apoptosis. The PERK pathway is the most important and also includes the phosphorylation of eukaryotic translation initiation factor 2α (eIF2α) and ATF4. In this report, we studied the regulatory effects of miR-1283 and ATF4 on the PERK-eIF2α-ATF4 signaling pathway using human umbilical vein endothelial cells (HUVECs) and mice. Methodology/Principal Findings We verified the relationship between miR-1283 and ATF4 using a luciferase activity assay and observed the regulatory effects of miR-1283 and ATF4 on the PERK-eIF2α-ATF4 signaling pathway in vivo and in vitro. Conclusions/Significance ATF4 is a target gene of miR-1283, which regulates the PERK-eIF2α-ATF4 signaling pathway by inhibiting ATF4, and it plays a critical role in inducing injury in HUVECs and mouse heart tissue. PMID:27537404

  9. Viscosity in Saturn's rings

    NASA Technical Reports Server (NTRS)

    Lissauer, J. J.; Shu, F. H.; Cuzzi, J. N.

    1982-01-01

    The technique of estimating the viscosity in Saturn's rings from the damping rate of waves observed to be propagating within the rings is discussed. The wavetrains of attempts using spiral density waves as a diagnostic suffer significant complications that compromise the interpretations. A method that considers the damping of spiral bending waves was used to deduce a kinematic viscosity of 260 (+150, -100) sqcm/sec for the middle of the A ring where bending waves are excited by the 5:3 vertical resonance with Mimas. This value implies upper limits on the particle velocity dispersion and local ring thickness of 0.4 cm/sec and 30 m, respectively.

  10. The Transcription Factor ATF5 Mediates a Mammalian Mitochondrial UPR.

    PubMed

    Fiorese, Christopher J; Schulz, Anna M; Lin, Yi-Fan; Rosin, Nadine; Pellegrino, Mark W; Haynes, Cole M

    2016-08-01

    Mitochondrial dysfunction is pervasive in human pathologies such as neurodegeneration, diabetes, cancer, and pathogen infections as well as during normal aging. Cells sense and respond to mitochondrial dysfunction by activating a protective transcriptional program known as the mitochondrial unfolded protein response (UPR(mt)), which includes genes that promote mitochondrial protein homeostasis and the recovery of defective organelles [1, 2]. Work in Caenorhabditis elegans has shown that the UPR(mt) is regulated by the transcription factor ATFS-1, which is regulated by organelle partitioning. Normally, ATFS-1 accumulates within mitochondria, but during respiratory chain dysfunction, high levels of reactive oxygen species (ROS), or mitochondrial protein folding stress, a percentage of ATFS-1 accumulates in the cytosol and traffics to the nucleus where it activates the UPR(mt) [2]. While similar transcriptional responses have been described in mammals [3, 4], how the UPR(mt) is regulated remains unclear. Here, we describe a mammalian transcription factor, ATF5, which is regulated similarly to ATFS-1 and induces a similar transcriptional response. ATF5 expression can rescue UPR(mt) signaling in atfs-1-deficient worms requiring the same UPR(mt) promoter element identified in C. elegans. Furthermore, mammalian cells require ATF5 to maintain mitochondrial activity during mitochondrial stress and promote organelle recovery. Combined, these data suggest that regulation of the UPR(mt) is conserved from worms to mammals. PMID:27426517

  11. ATF2 High Availability Power Supplies

    SciTech Connect

    Bellomo, A; Lira, C.de; Lam, B.; MacNair, D.; White, G.; /SLAC

    2008-06-27

    ATF2 is an accelerator test facility modeled after the final focus beamline envisioned for the ILC. By the end of 2008, KEK plans to commission the ATF2 [1]. SLAC and OCEM collaborated on the design of 38 power systems for beamline magnets. The systems range in output power from 1.5 kW to 6 kW. Since high availability is essential for the success of the ILC, Collaborators employed an N+1 modular approach, allowing for redundancy and the use of a single power module rating. This approach increases the availability of the power systems. Common power modules reduces inventory and eases maintenance. Current stability requirements are as tight as 10 ppm. A novel, SLAC designed 20-bit Ethernet Power Supply Controller provides the required precision current regulation. In this paper, Collaborators present the power system design, the expected reliability, fault immunity features, and the methods for satisfying the control and monitoring challenges. Presented are test results and the status of the power systems.

  12. Coupling Measurements in ATF2 Extraction Line

    SciTech Connect

    Rimbault, Cecile; Kuroda, Shigeru; Tauchi, Toshiaki; Terunuma, Nobuhiro; White, Glen; Woodley, Mark; /SLAC

    2012-06-29

    The purpose of ATF2 is to deliver a beam with stable very small spotsizes as required for future linear colliders such as ILC or CLIC. To achieve that, precise controls of aberrations such as dispersion and coupling are necessary. Theoretically, the complete reconstruction of the beam matrix is possible from the measurements of horizontal, vertical and tilted beam sizes, combining skew quadrupole scans at several wire-scanner positions. Such measurements were performed in the extraction line (EXT) of ATF2 in May 2009. We present analysis results attempting to resolve the 4 x 4 beam matrix. We aimed to reconstruct the full beam matrix using only skew quadrupole scans at different EXT wire-scanner locations, with measurements of horizontal, vertical and two tilted beam size projections. Checking the coherence of the {sigma}{sub 13} reconstruction from the {sigma}{sub 80{sup o}} and the {sigma}{sub 100{sup o}} measurements was essential to perform this analysis. We have shown that reconstruction of the coupling element can not be performed independantly of the 4 x 4 diagonal ones since it leads to unphysical results. A more accurate and automatisable method was find, leading to physical beam matrix reconstruction, compatible with the measurements. Another analysis should be performed based on a larger number of data sets to minimise statistical errors.

  13. Analysis of KEK-ATF optics and coupling using orbit response matrix analysis

    SciTech Connect

    Wolski, A.; Nelson, J.; Ross, M.; Woodley, M.; Mishra, S.; /Fermilab

    2004-01-01

    LOCO is a code for analysis of the linear optics in a storage ring based on the closed orbit response to steering magnets. The analysis provides information on focusing errors, BPM gain and rotation errors, and local coupling. Here, we report the results of an application of LOCO to the KEK-ATF. Although the analysis appears to have provided useful information on the optics of the machine, it appears that one of the main aims of the study--to reduce the vertical emittance by correcting the local coupling--was not successful, and we discuss some possible reasons for this.

  14. Analysis of KEK-ATF Optics And Coupling Using Orbit Response Matrix Analysis

    SciTech Connect

    Wolski, A.; Nelson, J.; Ross, M.; Woodley, M.; Mishra, S.; /Fermilab

    2006-10-13

    LOCO is a code for analysis of the linear optics in a storage ring based on the closed orbit response to steering magnets. The analysis provides information on focusing errors, BPM gain and rotation errors, and local coupling. Here, we report the results of an application of LOCO to the KEK-ATF. Although the analysis appears to have provided useful information on the optics of the machine, it appears that one of the main aims of the study--to reduce the vertical emittance by correcting the local coupling--was not successful, and we discuss some possible reasons for this.

  15. ATF3 controls proliferation of osteoclast precursor and bone remodeling

    PubMed Central

    Fukasawa, Kazuya; Park, Gyujin; Iezaki, Takashi; Horie, Tetsuhiro; Kanayama, Takashi; Ozaki, Kakeru; Onishi, Yuki; Takahata, Yoshifumi; Yoneda, Yukio; Takarada, Takeshi; Kitajima, Shigetaka; Vacher, Jean; Hinoi, Eiichi

    2016-01-01

    Bone homeostasis is maintained by the sophisticated coupled actions of bone-resorbing osteoclasts and bone-forming osteoblasts. Here we identify activating transcription factor 3 (ATF3) as a pivotal transcription factor for the regulation of bone resorption and bone remodeling under a pathological condition through modulating the proliferation of osteoclast precursors. The osteoclast precursor-specific deletion of ATF3 in mice led to the prevention of receptor activator of nuclear factor-κB (RANK) ligand (RANKL)-induced bone resorption and bone loss, although neither bone volume nor osteoclastic parameter were markedly altered in these knockout mice under the physiological condition. RANKL-dependent osteoclastogenesis was impaired in vitro in ATF3-deleted bone marrow macrophages (BMM). Mechanistically, the deficiency of ATF3 impaired the RANKL-induced transient increase in cell proliferation of osteoclast precursors in bone marrow in vivo as well as of BMM in vitro. Moreover, ATF3 regulated cyclin D1 mRNA expression though modulating activator protein-1-dependent transcription in the osteoclast precursor, and the introduction of cyclin D1 significantly rescued the impairment of osteoclastogenesis in ATF3-deleted BMM. Therefore, these findings suggest that ATF3 could have a pivotal role in osteoclastogenesis and bone homeostasis though modulating cell proliferation under pathological conditions, thereby providing a target for bone diseases. PMID:27480204

  16. Measurement of damping of graphite expoxy materials

    NASA Technical Reports Server (NTRS)

    Crocker, M. J.

    1985-01-01

    During this period damping measurements were made on the cylindrical graphite epoxy specimen using the forced-vibration test method. The specimen was carefully mounted directly on the shaker through the supporting ring and the impedance head. This was done to simulate an idealized free-free boundary condition at the two edges. The damping ratio value and the natural frequency (first mode) obtained in this experiment were 0.13% and 508.75 Hz respectively. In order to check the damping induced by the supporting ring, measurements were made with the top half of the ring removed. The specimen then was supported only by the bottom half of the ring (half ring). It was observed that the natural frequency of the specimen (first mode) increased of a value of 552.5 Hz due to the influence of the reduced mass of the supporting ring. But the damping ratio value obtained was the same as that obtained with full supporting ring, namely 0.13%.

  17. ATF3 suppresses ESCC via downregulation of ID1

    PubMed Central

    Li, Jian; Yang, Zishan; Chen, Zhiuguo; Bao, Yonghua; Zhang, Huijuan; Fang, Xinhui; Yang, Wancai

    2016-01-01

    Esophageal cancer is one of the most prevalent forms of cancer and has a particularly high mortality rate due to early metastasis; however, the underlying mechanisms of its formation and progression remain unclear. The present study performed immunohistochemical analysis and observed that the expression of activating transcription factor 3 (ATF3) was reduced in esophageal squamous cell carcinoma (ESCC) in comparison with non-tumor adjacent tissues. By contrast, inhibitor of DNA binding 1 (ID1) was overexpressed in ESCC tissues, demonstrating an inverse correlation with ATF3 (P<0.01). In ESCC EC109 and KYSE450 cells lines, transfection with an ATF3-overexpression plasmid resulted in the inhibition of cell proliferation, motility and migration, which was associated with the induction of E-cadherin expression and inhibition of cyclin D1 and Twist. Notably, ATF3 exerted an inverse regulatory interaction with ID1. The results of the present study provide additional evidence of the tumor suppressive features of ATF3 and demonstrate a novel mechanism of ATF3-mediated inhibition of cancer metastasis in esophageal cancer. PMID:27602100

  18. Ringing wormholes

    SciTech Connect

    Konoplya, R.A.; Molina, C.

    2005-06-15

    We investigate the response of traversable wormholes to external perturbations through finding their characteristic frequencies and time-domain profiles. The considered solution describes traversable wormholes between the branes in the two brane Randall-Sundrum model and was previously found within Einstein gravity with a conformally coupled scalar field. The evolution of perturbations of a wormhole is similar to that of a black hole and represents damped oscillations (ringing) at intermediately late times, which are suppressed by power-law tails (proportional to t{sup -2} for monopole perturbations) at asymptotically late times.

  19. The ATF two-frequency correlation reflectometer

    SciTech Connect

    Hanson, G.R.; Wilgen, J.B.; Anabitarte, E.; Bell, J.D.; Harris, J.H.; Dunlap, J.L.; Thomas, C.E.

    1990-01-01

    The Advanced Toroidal Facility (ATF) density fluctuation reflectometer system consists of two individual reflectometers operating in the 30- to 40-GHz band. Each reflectometer consists of a tunable microwave source and a quadrature phase detector connected to the same antenna system. This arrangement allows two-frequency operation along the same radial chord for radial coherence measurements. The technique used in making radial coherence measurements is discussed and the results of such experiments are given. Initial experiments have shown high coherence when the frequencies of the two reflectometers are tuned close together and a clear loss of coherence as the radial separation of the cutoff layers is increased by increasing the frequency separation of the two reflectometers. Recent results have shown that local measurements of density fluctuations in plasmas with electron cyclotron heating (ECH) are possible and that detailed structure can be seen in the fluctuation spectra. In addition, radial correlation lengths have been found to be from 0.5 to 1.0 cm in ECH plasmas, with some frequency structures having correlation lengths up to 3 cm. In plasmas with neutral beam injection (NBI), the radial correlation lengths in the edge region have been found to be approximately 0.1--0.2 cm. 4 figs.

  20. ATF4-dependent Regulation of the JMJD3 Gene during Amino Acid Deprivation Can Be Rescued in Atf4-deficient Cells by Inhibition of Deacetylation*

    PubMed Central

    Shan, Jixiu; Fu, Lingchen; Balasubramanian, Mukundh N.; Anthony, Tracy; Kilberg, Michael S.

    2012-01-01

    Following amino acid deprivation, the amino acid response (AAR) induces transcription from specific genes through a collection of signaling mechanisms, including the GCN2-eIF2-ATF4 pathway. The present report documents that the histone demethylase JMJD3 is an activating transcription factor 4 (ATF4)-dependent target gene. The JMJD3 gene contains two AAR-induced promoter activities and chromatin immunoprecipitation (ChIP) analysis showed that the AAR leads to enhanced ATF4 recruitment to the C/EBP-ATF response element (CARE) upstream of Promoter-1. AAR-induced histone modifications across the JMJD3 gene locus occur upon ATF4 binding. Jmjd3 transcription is not induced in Atf4-knock-out cells, but the AAR-dependent activation was rescued by inhibition of histone deacetylation with trichostatin A (TSA). The TSA rescue of AAR activation in the absence of Atf4 also occurred for the Atf3 and C/EBP homology protein (Chop) genes, but not for the asparagine synthetase gene. ChIP analysis of the Jmjd3, Atf3, and Chop genes in Atf4 knock-out cells documented that activation of the AAR in the presence of TSA led to specific changes in acetylation of histone H4. The results suggest that a primary function of ATF4 is to recruit histone acetyltransferase activity to a sub-set of AAR target genes. Thus, absolute binding of ATF4 to these particular genes is not required and no ATF4 interaction with the general transcription machinery is necessary. The data are consistent with the hypothesis that ATF4 functions as a pioneer factor to alter chromatin structure and thus, enhance transcription in a gene-specific manner. PMID:22955275

  1. ATF7 ablation prevents diet-induced obesity and insulin resistance.

    PubMed

    Liu, Yang; Maekawa, Toshio; Yoshida, Keisuke; Furuse, Tamio; Kaneda, Hideki; Wakana, Shigeharu; Ishii, Shunsuke

    2016-09-16

    The activating transcription factor (ATF)2 family of transcription factors regulates a variety of metabolic processes, including adipogenesis and adaptive thermogenesis. ATF7 is a member of the ATF2 family, and mediates epigenetic changes induced by environmental stresses, such as social isolation and pathogen infection. However, the metabolic role of ATF7 remains unknown. The aim of the present study is to examine the role of ATF7 in metabolism using ATF7-dificeint mice. Atf7(-/-) mice exhibited lower body weight and resisted diet-induced obesity. Serum triglycerides, resistin, and adipose tissue mass were all significantly lower in ATF7-deficient mice. Fasting glucose levels and glucose tolerance were unaltered, but systemic insulin sensitivity was increased, by ablation of ATF7. Indirect calorimetry revealed that oxygen consumption by Atf7(-/-) mice was comparable to that of wild-type littermates on a standard chow diet, but increased energy expenditure was observed in Atf7(-/-) mice on a high-fat diet. Hence, ATF7 ablation may impair the development and function of adipose tissue and result in elevated energy expenditure in response to high-fat-feeding obesity and insulin resistance, indicating that ATF7 is a potential therapeutic target for diet-induced obesity and insulin resistance. PMID:27498002

  2. High-brightness electron beam diagnostics at the ATF

    SciTech Connect

    Wang, X.J.; Ben-Zvi, I.

    1996-07-01

    The Brookhaven Accelerator Test Facility (ATF) is a dedicated user facility for accelerator physicists. Its design is optimized to explore laser acceleration and coherent radiation production. To characterize the low-emittance, picoseconds long electron beam produced by the ATF`s photocathode RF gun, we have installed electron beam profile monitors for transverse emittance measurement, and developed a new technique to measure electron beam pulse length by chirping the electron beam energy. We have also developed a new technique to measure the ps slice emittance of a 10 ps long electron beam. Stripline beam position monitors were installed along the beam to monitor the electron beam position and intensity. A stripline beam position monitor was also used to monitor the timing jitter between the RF system and laser pulses. Transition radiation was used to measure electron beam energy, beam profile and electron beam bunch length.

  3. Solution Accounts for Structural Damping

    NASA Technical Reports Server (NTRS)

    Roussos, L. A.; Hyer, M. W.; Thornton, E. A.

    1982-01-01

    New analytical technique determines dynamic response of damped structures dominated by internal structural damping mechanisms. Though structural damping is often negligible compared with damping due to air friction and friction in joints, structural damping can be of major importance in structures having heavy damping treatments or in outer-space structures. Finite-element model includes nonlinear, nonviscous internal damping.

  4. ATF2 knockdown reinforces oxidative stress-induced apoptosis in TE7 cancer cells

    PubMed Central

    Walluscheck, Diana; Poehlmann, Angela; Hartig, Roland; Lendeckel, Uwe; Schönfeld, Peter; Hotz-Wagenblatt, Agnes; Reissig, Kathrin; Bajbouj, Khuloud; Roessner, Albert; Schneider-Stock, Regine

    2013-01-01

    Cancer cells showing low apoptotic effects following oxidative stress-induced DNA damage are mainly affected by growth arrest. Thus, recent studies focus on improving anti-cancer therapies by increasing apoptosis sensitivity. We aimed at identifying a universal molecule as potential target to enhance oxidative stress-based anti-cancer therapy through a switch from cell cycle arrest to apoptosis. A cDNA microarray was performed with hydrogen peroxide-treated oesophageal squamous epithelial cancer cells TE7. This cell line showed checkpoint activation via p21WAF1, but low apoptotic response following DNA damage. The potential target molecule was chosen depended on the following demands: it should regulate DNA damage response, cell cycle and apoptosis. As the transcription factor ATF2 is implicated in all these processes, we focused on this protein. We investigated checkpoint activation via ATF2. Indeed, ATF2 knockdown revealed ATF2-triggered p21WAF1 protein expression, suggesting p21WAF1 transactivation through ATF2. Using chromatin immunoprecipitation (ChIP), we identified a hitherto unknown ATF2-binding sequence in the p21WAF1 promoter. p-ATF2 was found to interact with p-c-Jun, creating the AP-1 complex. Moreover, ATF2 knockdown led to c-Jun downregulation. This suggests ATF2-driven induction of c-Jun expression, thereby enhancing ATF2 transcriptional activity via c-Jun-ATF2 heterodimerization. Notably, downregulation of ATF2 caused a switch from cell cycle arrest to reinforced apoptosis, presumably via p21WAF1 downregulation, confirming the importance of ATF2 in the establishment of cell cycle arrest. 1-Chloro-2,4-dinitrobenzene also led to ATF2-dependent G2/M arrest, suggesting that this is a general feature induced by oxidative stress. As ATF2 knockdown also increased apoptosis, we propose ATF2 as a target for combined oxidative stress-based anti-cancer therapies. PMID:23800081

  5. Crosstalk between ATF4 and MTA1/HDAC1 promotes osteosarcoma progression

    PubMed Central

    Wang, Jiang; Ren, Ye; Li, Mi; Li, Hao; Cai, Zhuo; Chu, Qian; Yang, Caihong

    2016-01-01

    The stress response gene activating transcription factor 4 (ATF4) is involved in metastatic behavior and cellular protection. Here we show that ATF4 is upregulated in osteosarcoma (OS) cell lines and patient clinical samples as compared to matched non-tumor tissue. Overexpression of ATF4 in OS cells promoted cell proliferation, migration and lung metastasis. Furthermore, the expression of ATF4 was markedly reduced in metastasis associated protein (MTA1) or histone deacetylase 1 (HDAC1) knockdown OS cells, but MTA1 overexpression increased the stability and activity of ATF4 protein via ATF4 deacetylation by HDAC1. ATF4 in turn enhanced the expression of MTA1 and HDAC1 at the transcription level, suggesting a positive feedback loop between ATF4 and MTA1/HDAC1. Clinically, the level of ATF4 was positively correlated with that of MTA1 in OS. Mice injected with ATF4-overexpressing cells exhibited a higher rate of tumor growth, and the average weight of these tumors was ×90% greater than the controls. Taken together, these data establish a direct correlation between ATF4-induced OS progression and MTA1/HDAC1-associated metastasis, and support the potential therapeutic value of targeting ATF4 in the treatment of OS. PMID:26797758

  6. Emittance reconstruction from measured beam sizes in ATF2 and perspectives for ILC

    NASA Astrophysics Data System (ADS)

    Faus-Golfe, A.; Navarro, J.; Fuster Martinez, N.; Resta Lopez, J.; Giner Navarro, J.

    2016-05-01

    The projected emittance (2D) and the intrinsic emittance (4D) reconstruction method by using the beam size measurements at different locations is analyzed in order to study analytically the conditions of solvability of the systems of equations involved in this process. Some conditions are deduced and discussed, and general guidelines about the locations of the measurement stations have been obtained to avoid unphysical results. The special case of the multi-Optical Transition Radiation system (m-OTR), made of four measurement stations, in the Extraction Line (EXT) of Accelerator Test Facility 2 (ATF2) has been simulated in much detail and compared with measurements. Finally a feasibility study of a multi-station system for fast transverse beam size measurement, emittance reconstruction and coupling correction in the Ring to Main Linac (RTML) of International Linear Collider (ILC) Diagnostic sections of the RTML has been discussed in detail.

  7. Magnetic Damping For Maglev

    DOE PAGESBeta

    Zhu, S.; Cai, Y.; Rote, D. M.; Chen, S. S.

    1998-01-01

    Magnetic damping is one of the important parameters that control the response and stability of maglev systems. An experimental study to measure magnetic damping directly is presented. A plate attached to a permanent magnet levitated on a rotating drum was tested to investigate the effect of various parameters, such as conductivity, gap, excitation frequency, and oscillation amplitude, on magnetic damping. The experimental technique is capable of measuring all of the magnetic damping coefficients, some of which cannot be measured indirectly.

  8. The transcription factor ATF4 regulates glucose metabolism in mice through its expression in osteoblasts

    PubMed Central

    Yoshizawa, Tatsuya; Hinoi, Eiichi; Jung, Dae Young; Kajimura, Daisuke; Ferron, Mathieu; Seo, Jin; Graff, Jonathan M.; Kim, Jason K.; Karsenty, Gerard

    2009-01-01

    The recent demonstration that osteoblasts have a role in controlling energy metabolism suggests that they express cell-specific regulatory genes involved in this process. Activating transcription factor 4 (ATF4) is a transcription factor that accumulates predominantly in osteoblasts, where it regulates virtually all functions linked to the maintenance of bone mass. Since Atf4–/– mice have smaller fat pads than littermate controls, we investigated whether ATF4 also influences energy metabolism. Here, we have shown, through analysis of Atf4–/–mice, that ATF4 inhibits insulin secretion and decreases insulin sensitivity in liver, fat, and muscle. Several lines of evidence indicated that this function of ATF4 occurred through its osteoblastic expression. First, insulin sensitivity is enhanced in the liver of Atf4–/– mice, but not in cultured hepatocytes from these mice. Second, mice overexpressing ATF4 in osteoblasts only [termed here α1(I)Collagen-Atf4 mice] displayed a decrease in insulin secretion and were insulin insensitive. Third, the α1(I)Collagen-Atf4 transgene corrected the energy metabolism phenotype of Atf4–/– mice. Fourth, and more definitely, mice lacking ATF4 only in osteoblasts presented the same metabolic abnormalities as Atf4–/– mice. Molecularly, ATF4 favored expression in osteoblasts of Esp, which encodes a product that decreases the bioactivity of osteocalcin, an osteoblast-specific secreted molecule that enhances secretion of and sensitivity to insulin. These results provide a transcriptional basis to the observation that osteoblasts fulfill endocrine functions and identify ATF4 as a regulator of most functions of osteoblasts. PMID:19726872

  9. Damped leaf flexure hinge

    NASA Astrophysics Data System (ADS)

    Chen, Zhong; Chen, Guisheng; Zhang, Xianmin

    2015-05-01

    Flexure-based mechanism like compliant actuation system embeds complex dynamics that will reduce the control bandwidth and limits their dynamic positioning precision. This paper presents a theoretical model of a leaf flexure hinge with damping layers using strain energy method and Kelvin damping model. The modified loss factor of the damped leaf flexure hinge is derived, and the equivalent viscous damping coefficient of the damped leaf hinge is obtained, which could be used to improve the pseudo-rigid-model. The free vibration signals of the hinge in three different damping configurations are measured. The experimental modal analysis also is performed on the three kinds of damped leaf flexure hinges in order to evaluate their 1st order bending natural frequency and vibration-suppressing effects. The evaluation of modified loss factor model also is performed. The experimental results indicate that the constrained layer damping can enhance the structure damping of the hinge even if only single damping layer each side, the modified loss factor model can get good predicts of a damped leaf flexure hinge in the frequency range below 1st order natural frequency, and it is necessary that the dimensional parameters of the damping layers and basic layer of the hinge should be optimized for simplification at the mechanism's design stage.

  10. Damped leaf flexure hinge.

    PubMed

    Chen, Zhong; Chen, Guisheng; Zhang, Xianmin

    2015-05-01

    Flexure-based mechanism like compliant actuation system embeds complex dynamics that will reduce the control bandwidth and limits their dynamic positioning precision. This paper presents a theoretical model of a leaf flexure hinge with damping layers using strain energy method and Kelvin damping model. The modified loss factor of the damped leaf flexure hinge is derived, and the equivalent viscous damping coefficient of the damped leaf hinge is obtained, which could be used to improve the pseudo-rigid-model. The free vibration signals of the hinge in three different damping configurations are measured. The experimental modal analysis also is performed on the three kinds of damped leaf flexure hinges in order to evaluate their 1st order bending natural frequency and vibration-suppressing effects. The evaluation of modified loss factor model also is performed. The experimental results indicate that the constrained layer damping can enhance the structure damping of the hinge even if only single damping layer each side, the modified loss factor model can get good predicts of a damped leaf flexure hinge in the frequency range below 1st order natural frequency, and it is necessary that the dimensional parameters of the damping layers and basic layer of the hinge should be optimized for simplification at the mechanism's design stage. PMID:26026549

  11. A Laser-Wire System at the ATF Extraction Line

    SciTech Connect

    Boogert, S.T.; Blair, G.; Boorman, G.; Bosco, A.; Deacon, L.; Driouichi, C.; Karataev, P.; Kamps, T.; Delerue, N.; Dixit, S.; Foster, B.; Gannaway, F.; Howell, D.F.; Qureshi, M.; Reichold, A.; Senanayake, R.; Aryshev, A.; Hayano, H.; Kubo, K.; Terunuma, N.; Urakawa, J.; /KEK, Tsukuba /Liverpool U. /Cockcroft Inst. Accel. Sci. Tech. /SLAC

    2007-02-12

    A new laser-wire (LW) system has been installed at the ATF extraction line at KEK, Tsukuba. The system aims at a micron-scale laser spot size and employs a mode-locked laser system. The purpose-built interaction chamber, light delivery optics, and lens systems are described, and the first results are presented.

  12. The ATF (Advanced Toroidal Facility) Status and Control System

    SciTech Connect

    Baylor, L.R.; Devan, W.R.; Sumner, J.N.; Alban, A.M.

    1987-01-01

    The Advanced Toroidal Facility (ATF) Status and Control System (SCS) is a programmable controller-based state monitoring and supervisory control system. This paper describes the SCS implementation and its use of a host computer to run a commercially available software package that provides color graphic interactive displays, alarm logging, and archiving of state data.

  13. Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element.

    PubMed

    Crawford, Rebecca R; Prescott, Eugenia T; Sylvester, Charity F; Higdon, Ashlee N; Shan, Jixiu; Kilberg, Michael S; Mungrue, Imran N

    2015-06-19

    Using an unbiased systems genetics approach, we previously predicted a role for CHAC1 in the endoplasmic reticulum stress pathway, linked functionally to activating transcription factor 4 (ATF4) following treatment with oxidized phospholipids, a model for atherosclerosis. Mouse and yeast CHAC1 homologs have been shown to degrade glutathione in yeast and a cell-free system. In this report, we further defined the ATF4-CHAC1 interaction by cloning the human CHAC1 promoter upstream of a luciferase reporter system for in vitro assays in HEK293 and U2OS cells. Mutation and deletion analyses defined two major cis DNA elements necessary and sufficient for CHAC1 promoter-driven luciferase transcription under conditions of ER stress or ATF4 coexpression: the -267 ATF/cAMP response element (CRE) site and a novel -248 ATF/CRE modifier (ACM) element. We also examined the ability of the CHAC1 ATF/CRE and ACM sequences to bind ATF4 and ATF3 using immunoblot-EMSA and confirmed ATF4, ATF3, and CCAAT/enhancer-binding protein β binding at the human CHAC1 promoter in the proximity of the ATF/CRE and ACM using ChIP. To further validate the function of CHAC1 in a human cell model, we measured glutathione levels in HEK293 cells with enhanced CHAC1 expression. Overexpression of CHAC1 led to a robust depletion of glutathione, which was alleviated in a CHAC1 catalytic mutant. These results suggest an important role for CHAC1 in oxidative stress and apoptosis with implications for human health and disease. PMID:25931127

  14. Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element*

    PubMed Central

    Crawford, Rebecca R.; Prescott, Eugenia T.; Sylvester, Charity F.; Higdon, Ashlee N.; Shan, Jixiu; Kilberg, Michael S.; Mungrue, Imran N.

    2015-01-01

    Using an unbiased systems genetics approach, we previously predicted a role for CHAC1 in the endoplasmic reticulum stress pathway, linked functionally to activating transcription factor 4 (ATF4) following treatment with oxidized phospholipids, a model for atherosclerosis. Mouse and yeast CHAC1 homologs have been shown to degrade glutathione in yeast and a cell-free system. In this report, we further defined the ATF4-CHAC1 interaction by cloning the human CHAC1 promoter upstream of a luciferase reporter system for in vitro assays in HEK293 and U2OS cells. Mutation and deletion analyses defined two major cis DNA elements necessary and sufficient for CHAC1 promoter-driven luciferase transcription under conditions of ER stress or ATF4 coexpression: the −267 ATF/cAMP response element (CRE) site and a novel −248 ATF/CRE modifier (ACM) element. We also examined the ability of the CHAC1 ATF/CRE and ACM sequences to bind ATF4 and ATF3 using immunoblot-EMSA and confirmed ATF4, ATF3, and CCAAT/enhancer-binding protein β binding at the human CHAC1 promoter in the proximity of the ATF/CRE and ACM using ChIP. To further validate the function of CHAC1 in a human cell model, we measured glutathione levels in HEK293 cells with enhanced CHAC1 expression. Overexpression of CHAC1 led to a robust depletion of glutathione, which was alleviated in a CHAC1 catalytic mutant. These results suggest an important role for CHAC1 in oxidative stress and apoptosis with implications for human health and disease. PMID:25931127

  15. Ceapins inhibit ATF6α signaling by selectively preventing transport of ATF6α to the Golgi apparatus during ER stress

    PubMed Central

    Gallagher, Ciara M; Walter, Peter

    2016-01-01

    The membrane-bound transcription factor ATF6α is activated by proteolysis during endoplasmic reticulum (ER) stress. ATF6α target genes encode foldases, chaperones, and lipid biosynthesis enzymes that increase protein-folding capacity in response to demand. The off-state of ATF6α is maintained by its spatial separation in the ER from Golgi-resident proteases that activate it. ER stress induces trafficking of ATF6α. We discovered Ceapins, a class of pyrazole amides, as selective inhibitors of ATF6α signaling that do not inhibit the Golgi proteases or other UPR branches. We show that Ceapins block ATF6α signaling by trapping it in ER-resident foci that are excluded from ER exit sites. Removing the requirement for trafficking by pharmacological elimination of the spatial separation of the ER and Golgi apparatus restored cleavage of ATF6α in the presence of Ceapins. Washout of Ceapins resensitized ATF6α to ER stress. These results suggest that trafficking of ATF6α is regulated by its oligomeric state. DOI: http://dx.doi.org/10.7554/eLife.11880.001 PMID:27435962

  16. Ceapins inhibit ATF6α signaling by selectively preventing transport of ATF6α to the Golgi apparatus during ER stress.

    PubMed

    Gallagher, Ciara M; Walter, Peter

    2016-01-01

    The membrane-bound transcription factor ATF6α is activated by proteolysis during endoplasmic reticulum (ER) stress. ATF6α target genes encode foldases, chaperones, and lipid biosynthesis enzymes that increase protein-folding capacity in response to demand. The off-state of ATF6α is maintained by its spatial separation in the ER from Golgi-resident proteases that activate it. ER stress induces trafficking of ATF6α. We discovered Ceapins, a class of pyrazole amides, as selective inhibitors of ATF6α signaling that do not inhibit the Golgi proteases or other UPR branches. We show that Ceapins block ATF6α signaling by trapping it in ER-resident foci that are excluded from ER exit sites. Removing the requirement for trafficking by pharmacological elimination of the spatial separation of the ER and Golgi apparatus restored cleavage of ATF6α in the presence of Ceapins. Washout of Ceapins resensitized ATF6α to ER stress. These results suggest that trafficking of ATF6α is regulated by its oligomeric state. PMID:27435962

  17. Mutation of ATF6 causes autosomal recessive achromatopsia.

    PubMed

    Ansar, Muhammad; Santos-Cortez, Regie Lyn P; Saqib, Muhammad Arif Nadeem; Zulfiqar, Fareeha; Lee, Kwanghyuk; Ashraf, Naeem Mahmood; Ullah, Ehsan; Wang, Xin; Sajid, Sundus; Khan, Falak Sher; Amin-ud-Din, Muhammad; Smith, Joshua D; Shendure, Jay; Bamshad, Michael J; Nickerson, Deborah A; Hameed, Abdul; Riazuddin, Saima; Ahmed, Zubair M; Ahmad, Wasim; Leal, Suzanne M

    2015-09-01

    Achromatopsia (ACHM) is an early-onset retinal dystrophy characterized by photophobia, nystagmus, color blindness and severely reduced visual acuity. Currently mutations in five genes CNGA3, CNGB3, GNAT2, PDE6C and PDE6H have been implicated in ACHM. We performed homozygosity mapping and linkage analysis in a consanguineous Pakistani ACHM family and mapped the locus to a 15.12-Mb region on chromosome 1q23.1-q24.3 with a maximum LOD score of 3.6. A DNA sample from an affected family member underwent exome sequencing. Within the ATF6 gene, a single-base insertion variant c.355_356dupG (p.Glu119Glyfs*8) was identified, which completely segregates with the ACHM phenotype within the family. The frameshift variant was absent in public variant databases, in 130 exomes from unrelated Pakistani individuals, and in 235 ethnically matched controls. The variant is predicted to result in a truncated protein that lacks the DNA binding and transmembrane domains and therefore affects the function of ATF6 as a transcription factor that initiates the unfolded protein response during endoplasmic reticulum (ER) stress. Immunolabeling with anti-ATF6 antibodies showed localization throughout the mouse neuronal retina, including retinal pigment epithelium, photoreceptor cells, inner nuclear layer, inner and outer plexiform layers, with a more prominent signal in retinal ganglion cells. In contrast to cytoplasmic expression of wild-type protein, in heterologous cells ATF6 protein with the p.Glu119Glyfs*8 variant is mainly confined to the nucleus. Our results imply that response to ER stress as mediated by the ATF6 pathway is essential for color vision in humans. PMID:26063662

  18. Role of ATF5 in the invasive potential of diverse human cancer cell lines.

    PubMed

    Nukuda, Akihiro; Endoh, Hiroki; Yasuda, Motoaki; Mizutani, Takeomi; Kawabata, Kazushige; Haga, Hisashi

    2016-06-01

    Activating transcription factor 5 (ATF5) is a member of the ATF/cAMP response element-binding protein family. Our research group recently revealed that ATF5 expression increases the invasiveness of human lung carcinoma cells. However, the effects of ATF5 on the invasive potential of other cancer cells lines remain unclear. Therefore, in this study, we investigated the role of ATF5 in the invasive activity of diverse human cancer cell lines. Invasiveness was assessed using Matrigel invasion assays. ATF5 knockdown resulted in decreased invasiveness in seven of eight cancer cell lines tested. These results suggest that ATF5 promotes invasiveness in several cancer cell lines. Furthermore, the roles of ATF5 in the invasiveness were evaluated in three-dimensional (3D) culture conditions. In 3D collagen gel, HT-1080 and MDA-MB-231 cells exhibited high invasiveness, with spindle morphology and high invasion speed. In both cell lines, knockdown of ATF5 resulted in rounded morphology and decreased invasion speed. Next, we showed that ATF5 induced integrin-α2 and integrin-β1 expression and that the depletion of integrin-α2 or integrin-β1 resulted in round morphology and decreased invasion speed. Our results suggest that ATF5 promotes invasion by inducing the expression of integrin-α2 and integrin-β1 in several human cancer cell lines. PMID:27125458

  19. External Coulomb-Friction Damping For Hydrostatic Bearings

    NASA Technical Reports Server (NTRS)

    Buckmann, Paul S.

    1992-01-01

    External friction device damps vibrations of shaft and hydrostatic ring bearing in which it turns. Does not rely on wear-prone facing surfaces. Hydrostatic bearing ring clamped in radially flexing support by side plates clamped against radial surfaces by spring-loaded bolts. Plates provide friction against radial motions of shaft.

  20. Planetary Rings

    NASA Astrophysics Data System (ADS)

    Esposito, Larry

    2014-03-01

    Preface: a personal view of planetary rings; 1. Introduction: the allure of the ringed planets; 2. Studies of planetary rings 1610-2013; 3. Diversity of planetary rings; 4. Individual ring particles and their collisions; 5. Large-scale ring evolution; 6. Moons confine and sculpt rings; 7. Explaining ring phenomena; 8. N-body simulations; 9. Stochastic models; 10. Age and evolution of rings; 11. Saturn's mysterious F ring; 12. Uranus' rings and moons; 13. Neptune's partial rings; 14. Jupiter's ring-moon system after Galileo and New Horizons; 15. Ring photometry; 16. Dusty rings; 17. Concluding remarks; Afterword; Glossary; References; Index.

  1. Spin-Damping in an RF atomic magnetometer

    NASA Astrophysics Data System (ADS)

    Alem, Orang; Romalis, Michael V.; Sauer, Karen L.

    2009-05-01

    Optically pumped atomic magnetometers have demonstrated an improved sensitivity over standard tuned coils for frequencies less than 50 MHz, making these radio-frequency (RF) magnetometers attractive for low-field NMR (for example, Budker and Romalis, Nature Physics 3, April 2007). Such magnetometers are often plagued by transient effects resulting in decreased sensitivity. The decay time of these transients, or ringing, can last for milliseconds, which is particularly detrimental for rapidly decaying NMR signals. We have found that actively damping the ringing of the atomic spins can significantly reduce such dead time. This spin-damping of the atomic transients is achieved through a negative feedback mechanism in which part of the optical signal during ringing is used to apply an RF field forcing the realignment of the atomic spins with the static magnetic field. We have successfully implemented spin-damping in 100 μs and recovered our femto-Tesla signal previously obscured by the ringing.

  2. ATF4 deficiency protects hepatocytes from oxidative stress via inhibiting CYP2E1 expression

    PubMed Central

    Wang, Chunxia; Li, Houkai; Meng, Qingshu; Du, Ying; Xiao, Fei; Zhang, Qian; Yu, Junjie; Li, Kai; Chen, Shanghai; Huang, Zhiying; Liu, Bin; Guo, Feifan

    2014-01-01

    Activating transcription factor (ATF) 4 is involved in the regulation of oxidative stress in fibroblasts and neurons. The role of ATF4 in hepatocytes, however, is unknown. The aim of this study was to investigate the role of ATF4 in hepatocytes in oxidative stress under a high-fat diet (HFD). Here, we showed that palmitate-stimulated reactive oxygen species (ROS) production and triglyceride (TG) accumulation is blocked by ATF4 deficiency in primary hepatocytes. Consistently, HFD-induced oxidative stress, TG accumulation and expression of cytochrome P450, family 2, subfamily, polypeptide 1 (CYP2E1) are also blocked by knocking down ATF4 expression in the mouse liver. This suggests that ATF4 might regulate oxidative stress viaCYP2E1 under an HFD. In addition, we observed that expression of CYP2E1 is indirectly regulated by ATF4 in a cAMP-responsive element binding protein (CREB)-dependent manner, which can directly activate the CYP2E1 promoter activity. Notably, ATF4-stimulated ROS production is inhibited in vivo by treatment with diallyl sulphide, a selective CYP2E1 inhibitor. Finally, we showed that ATF4 expression in the liver is responsible for the protective effects against HFD-induced CYP2E1 expression, oxidative stress, and TG accumulation. Taken together, these observations suggest that ATF4 is a novel regulator of oxidative stress as well as accumulation of TG in response to HFD. PMID:24373582

  3. Naringenin-Mediated ATF3 Expression Contributes to Apoptosis in Human Colon Cancer

    PubMed Central

    Song, Hun Min; Park, Gwang Hun; Eo, Hyun Ji; Jeong, Jin Boo

    2016-01-01

    Naringenin (NAR) as one of the flavonoids observed in grapefruit has been reported to exhibit an anti-cancer activity. Activating transcription factor 3 (ATF3) is associated with apoptosis in human colon cancer cells. This study was performed to investigate the molecular mechanism by which NAR stimulates ATF3 expression and apoptosis in human colon cancer cells. NAR reduced the cell viability and induced an apoptosis in human colon cancer cells. ATF3 overexpression increased NAR-mediated cleaved PARP, while ATF3 knockdown attenuated the cleavage of PARP by NAR. NAR increased ATF3 expression in both protein and mRNA level, and increased the luciferase activity of ATF3 promoter in a dose-dependent manner. The responsible region for ATF3 transcriptional activation by NAR is located between −317 and −148 of ATF3 promoter. p38 inhibition blocked NAR-mediated ATF3 expression, its promoter activation and apoptosis. The results suggest that NAR induces apoptosis through p38-dependent ATF3 activation in human colon cancer cells. PMID:26797111

  4. Identification and characterization of a mitochondrial unfolded protein response transcription factor ATFS-1 in Litopenaeus vannamei.

    PubMed

    Chen, Yong-Gui; Yue, Hai-Tao; Zhang, Ze-Zhi; Yuan, Feng-Hua; Bi, Hai-Tao; Yuan, Kai; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong

    2016-07-01

    A mitochondrial specific stress response termed mitochondrial unfolded protein response (UPR(mt)) is activated in responding to disturbance of protein homeostasis in mitochondria. The activating transcription factor associated with stress-1 (designated as ATFS-1) is the key regulator of UPR(mt). To investigating the roles of ATFS-1 (LvATFS-1) in Litopenaeus vannamei mitochondrial stress remission and immunity, it's full length cDNA was cloned. The open reading frame of LvATFS-1 was 1, 557 bp in length, deducing to a 268 amino acids protein. LvATFS-1 was highly expressed in muscle, hemocytes and eyestalk. Subcellular location assays showed that N-terminal of LvATFS-1 contained a mitochondrial targeting sequence, which could directed the fused EGFP located to mitochondria. And the C-terminal of LvATFS-1, which had a nuclear localization signal, expressed in nucleus. The in vitro experiments verified that LvATFS-1 could reduced the level of intracellular reactive oxygen species (ROS). And results of real-time RT-PCR indicated that LvATFS-1 might scavenge excess ROS via ROS-eliminating genes regulation. Reporter gene assays showed that LvATFS-1 could upregulated the expression of the antimicrobial peptide genes in Drosophila Schneider 2 cells. Results of real-time RT-PCR showed that Vibrio alginolyticus or white spot syndrome virus (WSSV) infection induced the expression of LvATFS-1. And knocked-down LvATFS-1 by RNAi resulted in a higher cumulative mortality of L. vannamei upon V. alginolyticus or WSSV infection. These results suggested that LvATFS-1 not only rolled in mitochondrial specific stress responding, but also important for L. vannamei immunologic defence. PMID:26481519

  5. RMS active damping augmentation

    NASA Technical Reports Server (NTRS)

    Gilbert, Michael G.; Scott, Michael A.; Demeo, Martha E.

    1992-01-01

    The topics are presented in viewgraph form and include: RMS active damping augmentation; potential space station assembly benefits to CSI; LaRC/JSC bridge program; control law design process; draper RMS simulator; MIMO acceleration control laws improve damping; potential load reduction benefit; DRS modified to model distributed accelerations; accelerometer location; Space Shuttle aft cockpit simulator; simulated shuttle video displays; SES test goals and objectives; and SES modifications to support RMS active damping augmentation.

  6. Beam Compression Experiments using the UCLA/ATF Compressor

    SciTech Connect

    Rosenzweig, J. B.; Dunning, M.; Hemsing, E.; Andonian, G.; Cook, A. M.; Murokh, A.; Reiche, S.; Schiller, D.; Babzien, M.; Kusche, K.; Yakimenko, V.; Palumbo, L.; Vicario, C.

    2006-11-27

    We review recent experimental results from the BNL ATF using the compressor built by UCLA. The measurements discussed include: first observation of short wavelength coherent edge radiation angular/wavelength spectrum and spectrum, sub-100 fsec pulse-length coherent transition autocorrelation measurements, and longitudinal and transverse phase space distortions. Extension of these measurements, as well as those which can be made possible by a new X-band traveling wave deflector being developed in an industrial collaboration, are examined.

  7. Magnetic damping for maglev

    SciTech Connect

    Chen, S.S.; Zhu, S.; Cai, Y.; Rote, D.M.

    1994-12-31

    Magnetic damping is one of the important parameters to control the response and stability of maglev systems. An experimental study is presented to measure the magnetic damping using a direct method. A plate attached to a permanent magnet levitated on a rotating drum was tested to investigate the effect of various parameters on magnetic damping such as conductivity, gap, excitation frequency, and oscillation amplitude. The experimental technique is capable of measuring all magnetic damping coefficients, some of which can not be measured by an indirect method.

  8. Cavity Beam Position Monitor System for ATF2

    SciTech Connect

    Boogert, Stewart; Boorman, Gary; Swinson, Christina; Ainsworth, Robert; Molloy, Stephen; Aryshev, Alexander; Honda, Yosuke; Tauchi, Toshiaki; Terunuma, Nobuhiro; Urakawa, Junji; Frisch, Josef; May, Justin; McCormick, Douglas; Nelson, Janice; Smith, Tonee; White, Glen; Woodley, Mark; Heo, Ae-young; Kim, Eun-San; Kim, Hyoung-Suk; Kim, Youngim; /Kyungpook Natl. U. /University Coll. London /Kyungpook Natl. U. /Fermilab /Pohang Accelerator Lab.

    2012-07-09

    The Accelerator Test Facility 2 (ATF2) in KEK, Japan, is a prototype scaled demonstrator system for the final focus required for a future high energy lepton linear collider. The ATF2 beam-line is instrumented with a total of 38 C and S band resonant cavity beam position monitors (CBPM) with associated mixer electronics and digitizers. The current status of the BPM system is described, with a focus on operational techniques and performance. The ATF2 C-band system is performing well, with individual CBPM resolution approaching or at the design resolution of 50 nm. The changes in the CBPM calibration observed over three weeks can probably be attributed to thermal effects on the mixer electronics systems. The CW calibration tone power will be upgraded to monitor changes in the electronics gain and phase. The four S-band CBPMs are still to be investigated, the main problem associated with these cavities is a large cross coupling between the x and y ports. This combined with the large design dispersion in that degion makes the digital signal processing difficult, although various techniques exist to determine the cavity parameters and use these coupled signals for beam position determination.

  9. Impurity and recycling control with gettering in ATF

    SciTech Connect

    Simpkins, J.E.; Mioduszewski, P.K.; Isler, R.C.

    1991-01-01

    The vacuum vessel of the Advanced Toroidal Facility (ATF) is Ti-gettered with a surface coverage of 70%. The major effects of gettering are: (1) reduction of the oxygen, carbon, and nitrogen content in the plasma and (2) improved density control due to wall pumping of the working gas hydrogen. The overall leak rate in ATF is 2{times}10{sup {minus}4} Torr-l/s which is too high for successful plasma operation. Ti-gettering is routinely employed every morning prior to operation and compensates for this shortcoming by reducing the partial pressure of nitrogen and other residual gas components to the low 10{sup {minus}9} Torr range which is close to the RGA background pressure. Rate-of-rise measurements at this stage show only argon and some methane. The argon is used to monitor the leak rate. In addition to impurity reduction, gettering leads to low recycling of the working gas which appears to be crucial for density control in ATF. The capacity of the gettered surface is large enough to show a strong effect even after 24 hours. An extensive data base on the short-term and long-term effects of gettering on the residual gas composition and its effects on plasma performance has been established over the past three years and will be discussed in this paper. 9 refs., 7 figs.

  10. Computer simulation of magnetic field circuits in ATF

    SciTech Connect

    White, J.A.; Googe, J.M.; Nickels, L.E.

    1983-01-01

    The proposed design of the Advanced Toroidal Facility (ATF) contains several closely coupled magnetic field circuits that are being modeled using the SUPER*SCEPTRE computer program in order to predict their transient behavior. The results of this transient analysis study will be used to determine component values and/or special precautions that may be required for power supply and other circuit element protection due to the mutual coupling between circuits. ATF is a continuous-coil torsatron device using resistive coils in a pulsed mode of operation in which a current fluctuation in one coil induces voltages in the other circuit element protection due to the mutual coupling between circuits. ATF is a continuous-coil torsatron device using resistive coils in a pulsed mode of operation in which a current fluctuation in one coil induces voltages in the other circuit that may not be desirable. The model contains the solid-state power supplies' equivalent circuits, the resistance and self-inductance of each magnetic field coil, and the mutual inductances of every coil combination. The SUPER*SCEPTRE program allows for the direct input of all electrical components as well as the mutual inductances. The power supply voltages are entered as preprogrammed wave shapes designed to achieve the desired magnetic field strengths. The outputs of this program are tables and plots of voltages and currents associated with each circuit component.

  11. ATF4 is involved in the regulation of simulated microgravity induced integrated stress response

    NASA Astrophysics Data System (ADS)

    Li, Yingxian; Li, Qi; Wang, Xiaogang; Sun, Qiao; Wan, Yumin; Li, Yinghui; Bai, Yanqiang

    Objective: Many important metabolic and signaling pathways have been identified as being affected by microgravity, thereby altering cellular functions such as proliferation, differentiation, maturation and cell survival. It has been demonstrated that microgravity could induce all kinds of stress response such as endoplasmic reticulum stress and oxidative stress et al. ATF4 belongs to the ATF/CREB family of basic region leucine zipper transcription factors. ATF4 is induced by stress signals including anoxia/hypoxia, ER stress, amino acid deprivation and oxidative stress. ATF4 regulates the expression of genes involved in oxidative stress, amino acid synthesis, differentiation, metastasis and angiogenesis. The aim of this study was to examine the changes of ATF4 under microgravity, and to investigate the role of ATF4 in microgravity induced stress. MethodsMEF cells were cultured in clinostat to simulate microgravity. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to examine mRNA and protein levels of ATF4 expression under simulated microgravity in MEF cells. ROS levels were measured with the use of the fluorescent signal H2DCF-DA. GFP-XBP1 stably transfected cell lines was used to detect the extent of ER stress under microgravity by the intensity of GFP. Dual luciferase reporter assay was used to detect the activity of ATF4. Co-immunoprecipitation was performed to analyze protein interaction. Results: ATF4 protein levels in MEF cells increased under simulated microgravity. However, ATF4 mRNA levels were consistent. XBP1 splicing can be induced due to ER stress caused by simulated microgravity. At the same time, ROS levels were also increased. Increased ATF4 could promote the expression of CHOP, which is responsible for cell apoptosis. ATF4 also play an important role in cellular anti-oxidant stress. In ATF4 -/-MEF cells, the ROS levels after H2O2 treatment were obviously higher than that of wild type cells. HDAC4 was

  12. Decoherence and Landau-Damping

    SciTech Connect

    Ng, K.Y.; /Fermilab

    2005-12-01

    The terminologies, decoherence and Landau damping, are often used concerning the damping of a collective instability. This article revisits the difference and relation between decoherence and Landau damping. A model is given to demonstrate how Landau damping affects the rate of damping coming from decoherence.

  13. Suppression of X-rays generated by runaway electrons in ATF

    NASA Astrophysics Data System (ADS)

    Rasmussen, D. A.; England, A. C.; Eberle, C. C.; Devan, W. R.; Harris, J. H.; Jernigan, T. C.; Kindsfather, R. R.; Morris, R. N.; Murakami, M.; Neilson, G. H.

    X-ray emission from runaway electrons on ATF is a serious issue. Runaway suppression techniques used on Heliotron-E are not adequate for ATF. Three approaches have been developed to suppress runaway production. Monitoring devices have been installed in occupied areas and personnel access and exposure will be limited. Additional shielding will be added as required. These systems will be ready for installation and testing on ATF prior to commissioning or first plasma operation.

  14. The FONT5 Bunch-by-Bunch Position and Angle Feedback System at ATF2

    NASA Astrophysics Data System (ADS)

    Apsimon, R. J.; Bett, D. R.; Burrows, P. N.; Christian, G. B.; Constance, B.; Davis, M. R.; Gerbershagen, A.; Perry, C.; Resta-Lopez, J.

    The FONT5 upstream beam-based feedback system at ATF2 is designed to correct the position and angle jitter at the entrance to the ATF2 final-focus system, and also to demonstrate a prototype intra-train feedback system for the International Linear Collider interaction point. We discuss the hardware, from stripline BPMs to kickers, and RF and digital signal processing, as well as presenting results from the latest beam tests at ATF2.

  15. Turbine blade damping study

    NASA Technical Reports Server (NTRS)

    Dominic, R. J.

    1984-01-01

    Research results and progress on the performance of bladed systems is reported the different topics discussed include: the study of turbine blade damping; forced vibrations of friction damped beam moistures in two dimensions; and a users manual for a computer program for dynamic analysis of bladed systems.

  16. Damped acceleration cavities

    SciTech Connect

    Palmer, R.B.

    1988-07-01

    Structures with slots to strongly damp higher order longitudinal and transverse modes should allow the use, in linear colliders, of multiple bunches, and thus attain luminosities of over 10/sup 34/cm/sup /minus/2/sec/sup /minus/1/. Preliminary measurements on model structures suggest that such damping can be achieved. 10 refs., 9 figs.

  17. Planetary Rings

    NASA Astrophysics Data System (ADS)

    Esposito, Larry W.

    2011-07-01

    Preface; 1. Introduction: the allure of ringed planets; 2. Studies of planetary rings 1610-2004; 3. Diversity of planetary rings; 4. Individual ring particles and their collisions; 5. Large-scale ring evolution; 6. Moons confine and sculpt rings; 7. Explaining ring phenomena; 8. N-Body simulations; 9. Stochastic models; 10. Age and evolution of rings; 11. Saturn's mysterious F ring; 12. Neptune's partial rings; 13. Jupiter's ring-moon system after Galileo; 14. Ring photometry; 15. Dusty rings; 16. Cassini observations; 17. Summary: the big questions; Glossary; References; Index.

  18. Turbojet engine blade damping

    NASA Technical Reports Server (NTRS)

    Srinivasan, A. V.; Cutts, D. G.; Sridhar, S.

    1981-01-01

    The potentials of various sources of nonaerodynamic damping in engine blading are evaluated through a combination of advanced analysis and testing. The sources studied include material hysteresis, dry friction at shroud and root disk interfaces as well as at platform type external dampers. A limited seris of tests was conducted to evaluate damping capacities of composite materials (B/AL, B/AL/Ti) and thermal barrier coatings. Further, basic experiments were performed on titanium specimens to establish the characteristics of sliding friction and to determine material damping constants J and n. All the tests were conducted on single blades. Mathematical models were develthe several mechanisms of damping. Procedures to apply this data to predict damping levels in an assembly of blades are developed and discussed.

  19. Searching for novel ATF4 target genes in human hepatoma cells by microarray analysis.

    PubMed

    Maruyama, Ryuto; Shimizu, Makoto; Ishijima, Tomoko; Nakai, Yuji; Inoue, Jun; Sato, Ryuichiro

    2016-06-01

    Activating transcription factor 4 (ATF4) is a transcription factor with an important biological activity. ATF4 is induced by various stresses, such as endoplasmic reticulum stress, through the phosphorylation of eukaryotic translation initiation factor 2α. ATF4 is also involved in lipid metabolism. In the present study, we performed a microarray experiment to identify new ATF4 target genes, particularly those involved in lipid metabolism, and identified C12orf39, CSTA, and CALCB as novel ATF4 target genes. An amino acid response element (AARE) as an ATF4-binding site is present in the promoter regions of these genes. In a detailed analysis using luciferase assay, we showed that ATF4 activated C12orf39 promoter activity and that this activation was diminished by deletion or mutation of the AARE sequence in the promoter region. Our results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes and that C12orf39 promoter activity is activated by ATF4 through AARE. PMID:26967115

  20. ATF3 inhibits adipocyte differentiation of 3T3-L1 cells

    SciTech Connect

    Jang, Min Kyung; Kim, Cho Hee; Seong, Je Kyung; Jung, Myeong Ho

    2012-04-27

    Highlights: Black-Right-Pointing-Pointer Overexpression of ATF3 inhibits adipocyte differentiation in 3T3-L1 cells. Black-Right-Pointing-Pointer Overexpression of ATF3 represses C/EBP{alpha} expression. Black-Right-Pointing-Pointer ATF3 directly binds to mouse C/EBP{alpha} promoter spanning from -1928 to -1907. Black-Right-Pointing-Pointer ATF3 may play a role in hypoxia-mediated inhibition of adipocyte differentiation. -- Abstract: ATF3 is a stress-adaptive gene that regulates proliferation or apoptosis under stress conditions. However, the role of ATF3 is unknown in adipocyte cells. Therefore, in this study, we investigated the functional role of ATF3 in adipocytes. Both lentivirus-mediated overexpression of ATF3 and stably-overexpressed ATF3 inhibited adipocyte differentiation in 3T3-L1 cells, as revealed by decreased lipid staining with oil red staining and reduction in adipogenic genes. Thapsigargin treatment and overexpression of ATF3 decreased C/EBP{alpha} transcript and repressed the activity of the 3.6-kb mouse C/EBP{alpha} promoter, demonstrating that ATF3 downregulates C/EBP{alpha} expression. Transfection studies using mutant constructs containing 5 Prime -deletions in the C/EBP{alpha} promoter revealed that a putative ATF/CRE element, GGATGTCA, is located between -1921 and -1914. Electrophoretic mobility shift assay and chromatin immunoprecipitation assay demonstrated that ATF3 directly binds to mouse C/EBP{alpha} promoter spanning from -1928 to -1907. Both chemical hypoxia-mimetics or physical hypoxia led to reduce the C/EBP{alpha} mRNA and repress the promoter activity of the C/EBP{alpha} gene, whereas increase ATF3 mRNA, suggesting that ATF3 may contribute to the inhibition of adipocyte differentiation in hypoxia through downregulation of C/EBP{alpha} expression. Collectively, these results demonstrate that ATF3 represses the C/EBP{alpha} gene, resulting in inhibition of adipocyte differentiation, and thus plays a role in hypoxia-mediated inhibition

  1. Glucose deprivation induces chemoresistance in colorectal cancer cells by increasing ATF4 expression

    PubMed Central

    Hu, Ya-Ling; Yin, Yuan; Liu, He-Yong; Feng, Yu-Yang; Bian, Ze-Hua; Zhou, Le-Yuan; Zhang, Ji-Wei; Fei, Bo-Jian; Wang, Yu-Gang; Huang, Zhao-Hui

    2016-01-01

    AIM: To investigate the role of activating transcription factor 4 (ATF4) in glucose deprivation (GD) induced colorectal cancer (CRC) drug resistance and the mechanism involved. METHODS: Chemosensitivity and apoptosis were measured under the GD condition. Inhibition of ATF4 using short hairpin RNA in CRC cells under the GD condition and in ATF4-overexpressing CRC cells was performed to identify the role of ATF4 in the GD induced chemoresistance. Quantitative real-time RT-PCR and Western blot were used to detect the mRNA and protein expression of drug resistance gene 1 (MDR1), respectively. RESULTS: GD protected CRC cells from drug-induced apoptosis (oxaliplatin and 5-fluorouracil) and induced the expression of ATF4, a key gene of the unfolded protein response. Depletion of ATF4 in CRC cells under the GD condition can induce apoptosis and drug re-sensitization. Similarly, inhibition of ATF4 in the ATF4-overexpressing CRC cells reintroduced therapeutic sensitivity and apoptosis. In addition, increased MDR1 expression was observed in GD-treated CRC cells. CONCLUSION: These data indicate that GD promotes chemoresistance in CRC cells through up-regulating ATF4 expression. PMID:27468213

  2. Damping models in elastography

    NASA Astrophysics Data System (ADS)

    McGarry, Matthew D. J.; Berger, Hans-Uwe; Van Houten, Elijah E. W.

    2007-03-01

    Current optimization based Elastography reconstruction algorithms encounter difficulties when the motion approaches resonant conditions, where the model does a poor job of approximating the real behavior of the material. Model accuracy can be improved through the addition of damping effects. These effects occur in-vivo due to the complex interaction between microstructural elements of the tissue; however reconstruction models are typically formulated at larger scales where the structure can be treated as a continuum. Attenuation behavior in an elastic continuum can be described as a mixture of inertial and viscoelastic damping effects. In order to develop a continuum damping model appropriate for human tissue, the behavior of each aspect of this proportional, or Rayleigh damping needs to be characterized. In this paper we investigate the nature of these various damping representations with a goal of best describing in-vivo behavior of actual tissue in order to improve the accuracy and performance of optimization based elastographic reconstruction. Inertial damping effects are modelled using a complex density, where the imaginary part is equivalent to a damping coefficient, and the effects of viscoelasticity are modelled through the use of complex shear moduli, where the real and imaginary parts represent the storage and loss moduli respectively. The investigation is carried out through a combination of theoretical analysis, numerical experiment, investigation of gelatine phantoms and comparison with other continua such as porous media models.

  3. Spatial cyclotron damping

    NASA Technical Reports Server (NTRS)

    Olson, C. L.

    1970-01-01

    To examine spatial electron cyclotron damping in a uniform Vlasov plasma, it is noted that the plasma response to a steady-state transverse excitation consists of several terms (dielectric-pole, free-streaming, and branch-cut), but that the cyclotron-damped pole term is the dominant term for z l = c/w sub ce provided (w sub pe/w sub ce) squared (c/a) is much greater than 1. If the latter inequality does not hold, then the free-streaming and branch-cut terms persist well past z = c/w sub ce as w sub 1 approaches w sub ce, making experimental measurement of cyclotron damping essentially impossible. Considering only (w sub pe/w sub ce) squared (c/a) is much greater than 1, it is shown how collisional effects should be estimated and how a finite-width excitation usually has little effect on the cyclotron-damped part of the response. Criteria is established concerning collisional damping, measurable damping length sizes, and allowed uncertainty in the magnetic field Beta. Results of numerical calculations, showing the regions in the appropriate parameter spaces that meet these criteria, are presented. From these results, one can determine the feasibility of, or propose parameter values for, an experiment designed to measure spatial cyclotron damping. It is concluded that the electron temperature T sub e should be at least 1 ev., and preferably 10 ev. or higher, for a successful experiment.

  4. Acoustic transducer with damping means

    DOEpatents

    Smith, Richard W.; Adamson, Gerald E.

    1976-11-02

    An ultrasonic transducer specifically suited to high temperature sodium applications is described. A piezoelectric active element is joined to the transducer faceplate by coating the faceplate and juxtaposed active element face with wetting agents specifically compatible with the bonding procedure employed to achieve the joint. The opposite face of the active element is fitted with a backing member designed to assure continued electrical continuity during adverse operating conditions which can result in the fracturing of the active element. The fit is achieved employing a spring-loaded electrode operably arranged to electrically couple the internal transducer components, enclosed in a hermetically sealed housing, to accessory components normally employed in transducer applications. Two alternative backing members are taught for assuring electrical continuity. The first employs a resilient, discrete multipoint contact electrode in electrical communication with the active element face. The second employs a resilient, elastomeric, electrically conductive, damped member in electrical communication with the active element face in a manner to effect ring-down of the transducer. Each embodiment provides continued electrical continuity within the transducer in the event the active element fractures, while the second provides the added benefit of damping.

  5. Development of the alternate entry port for the ATF

    SciTech Connect

    Parsa, Z.

    1993-05-01

    We discussed a second entry port for the Accelerator Test Facility (ATF) injection system at Brookhaven National Laboratory, which consists of a photocathode rf gun and a straight - ahead beamline directly into the 50 MeV linac. The proposed second entry port should improve the beam quality and lower the emittance needed for FEL (Free Electron Laser), and laser - acceleration experiments. A discussion on the laser driven high brightness photoelectrons through the primary entry port (a low energy 180 degrees achromatic double bend transport line) now in operation, and a beam analysis for the proposed secondary port is also given.

  6. Antenna Test Facility (ATF): User Test Planning Guide

    NASA Technical Reports Server (NTRS)

    Lin, Greg

    2011-01-01

    Test process, milestones and inputs are unknowns to first-time users of the ATF. The User Test Planning Guide aids in establishing expectations for both NASA and non-NASA facility customers. The potential audience for this guide includes both internal and commercial spaceflight hardware/software developers. It is intended to assist their test engineering personnel in test planning and execution. Material covered includes a roadmap of the test process, roles and responsibilities of facility and user, major milestones, facility capabilities, and inputs required by the facility. Samples of deliverables, test article interfaces, and inputs necessary to define test scope, cost, and schedule are included as an appendix to the guide.

  7. A Flight Simulator for ATF2 - A Mechanism for International Collaboration in the Writing and Deployment of Online Beam Dynamics Algorithms

    SciTech Connect

    White, Glen; Molloy, Stephen; Seryi, Andrei; Schulte, Daniel; Tomas, Rogelio; Kuroda, Shigeru; Bambade, Philip; Renier, Yves; /Orsay, LAL

    2008-07-25

    The goals of ATF2 are to test a novel compact final focus optics design with local chromaticity correction intended for use in future linear colliders. The newly designed extraction line and final focus system will be used to produce a 37nm vertical waist from an extracted beam from the ATF ring of {approx}30nm vertical normalized emittance, and to stabilize it at the IP-waist to the {approx}2nm level. Static and dynamic tolerances on all accelerator components are very tight; the achievement of the ATF2 goals is reliant on the application of multiple high-level beam dynamics control algorithms to align and tune the electron beam in the extraction line and final focus system. Much algorithmic development work has been done in Japan and by colleagues in collaborating nations in North America and Europe. We describe here development work towards realizing a 'flight simulator' environment for the shared development and implementation of beam dynamics code. This software exists as a 'middle-layer' between the lower-level control systems (EPICS and V-SYSTEM) and the multiple higher-level beam dynamics modeling tools in use by the three regions (SAD, Lucretia, PLACET, MAD...).

  8. DAMPs, ageing, and cancer: The 'DAMP Hypothesis'.

    PubMed

    Huang, Jin; Xie, Yangchun; Sun, Xiaofang; Zeh, Herbert J; Kang, Rui; Lotze, Michael T; Tang, Daolin

    2015-11-01

    Ageing is a complex and multifactorial process characterized by the accumulation of many forms of damage at the molecular, cellular, and tissue level with advancing age. Ageing increases the risk of the onset of chronic inflammation-associated diseases such as cancer, diabetes, stroke, and neurodegenerative disease. In particular, ageing and cancer share some common origins and hallmarks such as genomic instability, epigenetic alteration, aberrant telomeres, inflammation and immune injury, reprogrammed metabolism, and degradation system impairment (including within the ubiquitin-proteasome system and the autophagic machinery). Recent advances indicate that damage-associated molecular pattern molecules (DAMPs) such as high mobility group box 1, histones, S100, and heat shock proteins play location-dependent roles inside and outside the cell. These provide interaction platforms at molecular levels linked to common hallmarks of ageing and cancer. They can act as inducers, sensors, and mediators of stress through individual plasma membrane receptors, intracellular recognition receptors (e.g., advanced glycosylation end product-specific receptors, AIM2-like receptors, RIG-I-like receptors, and NOD1-like receptors, and toll-like receptors), or following endocytic uptake. Thus, the DAMP Hypothesis is novel and complements other theories that explain the features of ageing. DAMPs represent ideal biomarkers of ageing and provide an attractive target for interventions in ageing and age-associated diseases. PMID:25446804

  9. 27 CFR 646.153 - Authority of appropriate ATF officers to enter business premises.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... REGULATIONS RELATING TO ALCOHOL AND TOBACCO CONTRABAND CIGARETTES Other Provisions Relating to the Distribution of Cigarettes § 646.153 Authority of appropriate ATF officers to enter business premises. Any appropriate ATF officer may enter the business premises of any distributor of cigarettes to inspect...

  10. 27 CFR 646.153 - Authority of appropriate ATF officers to enter business premises.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... REGULATIONS RELATING TO ALCOHOL AND TOBACCO CONTRABAND CIGARETTES Other Provisions Relating to the Distribution of Cigarettes § 646.153 Authority of appropriate ATF officers to enter business premises. Any appropriate ATF officer may enter the business premises of any distributor of cigarettes to inspect...

  11. 27 CFR 646.153 - Authority of appropriate ATF officers to enter business premises.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... REGULATIONS RELATING TO ALCOHOL AND TOBACCO CONTRABAND CIGARETTES Other Provisions Relating to the Distribution of Cigarettes § 646.153 Authority of appropriate ATF officers to enter business premises. Any appropriate ATF officer may enter the business premises of any distributor of cigarettes to inspect...

  12. 27 CFR 646.153 - Authority of appropriate ATF officers to enter business premises.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... REGULATIONS RELATING TO ALCOHOL AND TOBACCO CONTRABAND CIGARETTES Other Provisions Relating to the Distribution of Cigarettes § 646.153 Authority of appropriate ATF officers to enter business premises. Any appropriate ATF officer may enter the business premises of any distributor of cigarettes to inspect...

  13. Functional Contribution of the Transcription Factor ATF4 to the Pathogenesis of Amyotrophic Lateral Sclerosis

    PubMed Central

    Matus, Soledad; Lopez, Estefanía; Valenzuela, Vicente; Nassif, Melissa; Hetz, Claudio

    2013-01-01

    Endoplasmic reticulum (ER) stress represents an early pathological event in amyotrophic lateral sclerosis (ALS). ATF4 is a key ER stress transcription factor that plays a role in both adaptation to stress and the activation of apoptosis. Here we investigated the contribution of ATF4 to ALS. ATF4 deficiency reduced the rate of birth of SOD1G86R transgenic mice. The fraction of ATF4−/−-SOD1G85R transgenic mice that were born are more resistant to develop ALS, leading to delayed disease onset and prolonged life span. ATF4 deficiency completely attenuated the induction of pro-apoptotic genes, including BIM and CHOP, and also led to quantitative changes in the ER protein homeostasis network. Unexpectedly, ATF4 deficiency enhanced mutant SOD1 aggregation at the end stage of the disease. Studies in the motoneuron cell line NSC34 demonstrated that knocking down ATF4 enhances mutant SOD1 aggregation possibly due to alteration in the redox status of the cell. Our results support a functional role of ATF4 in ALS, offering a novel target for disease intervention. PMID:23874395

  14. ATF4-dependent induction of heme oxygenase 1 prevents anoikis and promotes metastasis

    PubMed Central

    Dey, Souvik; Sayers, Carly M.; Verginadis, Ioannis I.; Lehman, Stacey L.; Cheng, Yi; Cerniglia, George J.; Tuttle, Stephen W.; Feldman, Michael D.; Zhang, Paul J.L.; Fuchs, Serge Y.; Diehl, J. Alan; Koumenis, Constantinos

    2015-01-01

    The integrated stress response (ISR) is a critical mediator of cancer cell survival, and targeting the ISR inhibits tumor progression. Here, we have shown that activating transcription factor 4 (ATF4), a master transcriptional effector of the ISR, protects transformed cells against anoikis — a specialized form of apoptosis — following matrix detachment and also contributes to tumor metastatic properties. Upon loss of attachment, ATF4 activated a coordinated program of cytoprotective autophagy and antioxidant responses, including induced expression of the major antioxidant enzyme heme oxygenase 1 (HO-1). HO-1 upregulation was the result of simultaneous activation of ATF4 and the transcription factor NRF2, which converged on the HO1 promoter. Increased levels of HO-1 ameliorated oxidative stress and cell death. ATF4-deficient human fibrosarcoma cells were unable to colonize the lungs in a murine model, and reconstitution of ATF4 or HO-1 expression in ATF4-deficient cells blocked anoikis and rescued tumor lung colonization. HO-1 expression was higher in human primary and metastatic tumors compared with noncancerous tissue. Moreover, HO-1 expression correlated with reduced overall survival of patients with lung adenocarcinoma and glioblastoma. These results establish HO-1 as a mediator of ATF4-dependent anoikis resistance and tumor metastasis and suggest ATF4 and HO-1 as potential targets for therapeutic intervention in solid tumors. PMID:26011642

  15. Damping of nanomechanical resonators.

    PubMed

    Unterreithmeier, Quirin P; Faust, Thomas; Kotthaus, Jörg P

    2010-07-01

    We study the transverse oscillatory modes of nanomechanical silicon nitride strings under high tensile stress as a function of geometry and mode index m≤9. Reproducing all observed resonance frequencies with classical elastic theory we extract the relevant elastic constants. Based on the oscillatory local strain we successfully predict the observed mode-dependent damping with a single frequency-independent fit parameter. Our model clarifies the role of tensile stress on damping and hints at the underlying microscopic mechanisms. PMID:20867737

  16. The Vibration Ring. Phase 1; [Seedling Fund

    NASA Technical Reports Server (NTRS)

    Asnani, Vivake M.; Krantz, Timothy L.; Delap, Damon C.; Stringer, David B.

    2014-01-01

    The vibration ring was conceived as a driveline damping device to prevent structure-borne noise in machines. It has the appearance of a metal ring, and can be installed between any two driveline components like an ordinary mechanical spacer. Damping is achieved using a ring-shaped piezoelectric stack that is poled in the axial direction and connected to an electrical shunt circuit. Surrounding the stack is a metal structure, called the compression cage, which squeezes the stack along its poled axis when excited by radial driveline forces. The stack in turn generates electrical energy, which is either dissipated or harvested using the shunt circuit. Removing energy from the system creates a net damping effect. The vibration ring is much stiffer than traditional damping devices, which allows it to be used in a driveline without disrupting normal operation. In phase 1 of this NASA Seedling Fund project, a combination of design and analysis was used to examine the feasibility of this concept. Several designs were evaluated using solid modeling, finite element analysis, and by creating prototype hardware. Then an analytical model representing the coupled electromechanical response was formulated in closed form. The model was exercised parametrically to examine the stiffness and loss factor spectra of the vibration ring, as well as simulate its damping effect in the context of a simplified driveline model. The results of this work showed that this is a viable mechanism for driveline damping, and provided several lessons for continued development.

  17. Atf3 mutant mice show reduced axon regeneration and impaired regeneration-associated gene induction after peripheral nerve injury.

    PubMed

    Gey, Manuel; Wanner, Renate; Schilling, Corinna; Pedro, Maria T; Sinske, Daniela; Knöll, Bernd

    2016-08-01

    Axon injury in the peripheral nervous system (PNS) induces a regeneration-associated gene (RAG) response. Atf3 (activating transcription factor 3) is such a RAG and ATF3's transcriptional activity might induce 'effector' RAGs (e.g. small proline rich protein 1a (Sprr1a), Galanin (Gal), growth-associated protein 43 (Gap43)) facilitating peripheral axon regeneration. We provide a first analysis of Atf3 mouse mutants in peripheral nerve regeneration. In Atf3 mutant mice, facial nerve regeneration and neurite outgrowth of adult ATF3-deficient primary dorsal root ganglia neurons was decreased. Using genome-wide transcriptomics, we identified a neuropeptide-encoding RAG cluster (vasoactive intestinal peptide (Vip), Ngf, Grp, Gal, Pacap) regulated by ATF3. Exogenous administration of neuropeptides enhanced neurite growth of Atf3 mutant mice suggesting that these molecules might be effector RAGs of ATF3's pro-regenerative function. In addition to the induction of growth-promoting molecules, we present data that ATF3 suppresses growth-inhibiting molecules such as chemokine (C-C motif) ligand 2. In summary, we show a pro-regenerative ATF3 function during PNS nerve regeneration involving transcriptional activation of a neuropeptide-encoding RAG cluster. ATF3 is a general injury-inducible factor, therefore ATF3-mediated mechanisms identified herein might apply to other cell and injury types. PMID:27581653

  18. Essential role of eIF5-mimic protein in animal development is linked to control of ATF4 expression

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Translational control of ATF4 through upstream ORFs (uORFs) plays an important role in eukaryotic gene regulation. While ATF4 translation is typically induced by inhibitory phosphorylation of eIF2, ATF4 translation can be also induced by expression of a new translational inhibitor protein, eIF5-mimi...

  19. Atf3 mutant mice show reduced axon regeneration and impaired regeneration-associated gene induction after peripheral nerve injury

    PubMed Central

    Gey, Manuel; Wanner, Renate; Schilling, Corinna; Pedro, Maria T.; Sinske, Daniela

    2016-01-01

    Axon injury in the peripheral nervous system (PNS) induces a regeneration-associated gene (RAG) response. Atf3 (activating transcription factor 3) is such a RAG and ATF3's transcriptional activity might induce ‘effector’ RAGs (e.g. small proline rich protein 1a (Sprr1a), Galanin (Gal), growth-associated protein 43 (Gap43)) facilitating peripheral axon regeneration. We provide a first analysis of Atf3 mouse mutants in peripheral nerve regeneration. In Atf3 mutant mice, facial nerve regeneration and neurite outgrowth of adult ATF3-deficient primary dorsal root ganglia neurons was decreased. Using genome-wide transcriptomics, we identified a neuropeptide-encoding RAG cluster (vasoactive intestinal peptide (Vip), Ngf, Grp, Gal, Pacap) regulated by ATF3. Exogenous administration of neuropeptides enhanced neurite growth of Atf3 mutant mice suggesting that these molecules might be effector RAGs of ATF3's pro-regenerative function. In addition to the induction of growth-promoting molecules, we present data that ATF3 suppresses growth-inhibiting molecules such as chemokine (C-C motif) ligand 2. In summary, we show a pro-regenerative ATF3 function during PNS nerve regeneration involving transcriptional activation of a neuropeptide-encoding RAG cluster. ATF3 is a general injury-inducible factor, therefore ATF3-mediated mechanisms identified herein might apply to other cell and injury types. PMID:27581653

  20. Damping modeling in Timoshenko beams

    NASA Technical Reports Server (NTRS)

    Banks, H. T.; Wang, Y.

    1992-01-01

    Theoretical and numerical results of damping model studies for composite material beams using the Timoshenko theory is presented. Based on the damping models developed for Euler-Bernoulli beams, the authors develop damping methods for both bending and shear in investigation of Timoshenko beams. A computational method for the estimation of the damping parameters is given. Experimental data with high-frequency excitation were used to test Timoshenko beam equations with different types of damping models for bending and shear in various combinations.

  1. Experiments with particle damping

    NASA Astrophysics Data System (ADS)

    Hollkamp, Joseph J.; Gordon, Robert W.

    1998-06-01

    High cycle fatigue in jet engines is a current military concern. The vibratory stresses that cause fatigue can be reduced by adding damping. However, the high temperatures that occur in the gas turbine greatly hinder the application of mature damping technologies. One technology which may perform in the harsh environment is particle damping. Particle damping involves placing metallic or ceramic particles inside structural cavities. As the cavity vibrates, energy is dissipated through particle collisions. Performance is influenced by many parameters including the type, shape, and size of the particles; the amount of free volume for the particles to move in; density of the particles; and the level of vibration. This paper presents results from a series of experiments designed to gain an appreciation of the important parameters. The experimental setup consists of a cantilever beam with drilled holes. These holes are partially filled with particles. The types of particles, location of the particles, fill level, and other parameters are varied. Damping is estimated for each configuration. Trends in the results are studied to determine the influence of the varied parameter.

  2. DAMPs and autophagy

    PubMed Central

    Zhang, Qiuhong; Kang, Rui; Zeh, III, Herbert J.; Lotze, Michael T.; Tang, Daolin

    2013-01-01

    Autophagy is a lysosome-mediated catabolic process involving the degradation of intracellular contents (e.g., proteins and organelles) as well as invading microbes (e.g., parasites, bacteria and viruses). Multiple forms of cellular stress can stimulate this pathway, including nutritional imbalances, oxygen deprivation, immunological response, genetic defects, chromosomal anomalies and cytotoxic stress. Damage-associated molecular pattern molecules (DAMPs) are released by stressed cells undergoing autophagy or injury, and act as endogenous danger signals to regulate the subsequent inflammatory and immune response. A complex relationship exists between DAMPs and autophagy in cellular adaption to injury and unscheduled cell death. Since both autophagy and DAMPs are important for pathogenesis of human disease, it is crucial to understand how they interplay to sustain homeostasis in stressful or dangerous environments. PMID:23388380

  3. Chronic ethanol consumption inhibits glucokinase transcriptional activity by Atf3 and triggers metabolic syndrome in vivo.

    PubMed

    Kim, Ji Yeon; Hwang, Joo-Yeon; Lee, Dae Yeon; Song, Eun Hyun; Park, Keon Jae; Kim, Gyu Hee; Jeong, Eun Ae; Lee, Yoo Jeong; Go, Min Jin; Kim, Dae Jin; Lee, Seong Su; Kim, Bong-Jo; Song, Jihyun; Roh, Gu Seob; Gao, Bin; Kim, Won-Ho

    2014-09-26

    Chronic ethanol consumption induces pancreatic β-cell dysfunction through glucokinase (Gck) nitration and down-regulation, leading to impaired glucose tolerance and insulin resistance, but the underlying mechanism remains largely unknown. Here, we demonstrate that Gck gene expression and promoter activity in pancreatic β-cells were suppressed by chronic ethanol exposure in vivo and in vitro, whereas expression of activating transcription factor 3 (Atf3) and its binding to the putative Atf/Creb site (from -287 to -158 bp) on the Gck promoter were up-regulated. Furthermore, in vitro ethanol-induced Atf3 inhibited the positive effect of Pdx-1 on Gck transcriptional regulation, enhanced recruitment of Hdac1/2 and histone H3 deacetylation, and subsequently augmented the interaction of Hdac1/Pdx-1 on the Gck promoter, which were diminished by Atf3 siRNA. In vivo Atf3-silencing reversed ethanol-mediated Gck down-regulation and β-cell dysfunction, followed by the amelioration of impaired glucose tolerance and insulin resistance. Together, we identified that ethanol-induced Atf3 fosters β-cell dysfunction via Gck down-regulation and that its loss ameliorates metabolic syndrome and could be a potential therapeutic target in treating type 2 diabetes. The Atf3 gene is associated with the induction of type 2 diabetes and alcohol consumption-induced metabolic impairment and thus may be the major negative regulator for glucose homeostasis. PMID:25074928

  4. Oxidative stress-responsive transcription factor ATF3 potentially mediates diabetic angiopathy.

    PubMed

    Okamoto, Aki; Iwamoto, Yasuhiko; Maru, Yoshiro

    2006-02-01

    Previous results of our cDNA microarray analysis to look for genes whose expression level correlates well with in vitro tubulogenesis by NP31 endothelial cells revealed the transcription factor ATF3 known to be responsive to stress such as reactive oxygen species (ROS). Anti-ATF3 small interfering RNA gave an inhibitory influence on tube formation by NP31 cells expressing an activated form of the vascular endothelial growth factor receptor 1 (VEGFR-1) kinase. When expression of ATF3 was regulated under the control of tetracycline system in NP31 cells, they acquired the tubulogenic ability upon ATF3 induction. While ATF3 failed to induce expressions of VEGF and VEGFR, it regulated those of CDK2, CDK4, p8, plasminogen activator inhibitor 1, integrin alpha1, subunit and matrix metalloprotease MMP13. In H2O2-stimulated NP31 cells as well as endothelial cells of glomerulus and aorta of Otsuka-Long-Evans-Tokushima-Fatty diabetic model rats, concomitantly enhanced expressions of ATF3, PAI-1, and p8 were observed. Given the proposed hypothesis of the close linkage between diabetic angiopathy and ROS, those data suggest that ROS-associated diabetic complication may involve ATF3-mediated pathological angiogenesis. PMID:16428460

  5. Atf3 negatively regulates Ptgs2/Cox2 expression during acute inflammation

    PubMed Central

    Hellmann, Jason; Tang, Yunan; Zhang, Michael J.; Hai, Tsonwin; Bhatnagar, Aruni; Srivastava, Sanjay; Spite, Matthew

    2015-01-01

    By generating prostaglandins, cyclooxygenase-2 (Cox-2/Ptgs2) plays a critical role in regulating inflammatory responses. While several inflammatory stimuli have been shown to increase Ptgs2 expression, less is known about how the transcription of this gene is terminated. Here we show that stimulation of macrophages with yeast zymosan, a TLR2/6 and dectin-1 agonist, causes a transient increase in the expression of Ptgs2 accompanied by a simultaneous increase in the expression of the transcriptional repressor, Activating transcription factor-3 (Atf3). The expression of Ptgs2 was significantly higher in resident peritoneal macrophages isolated from Atf3−/− mice than that from Atf3+/+ mice and was associated with higher prostaglandin production upon stimulation with zymosan. In activated macrophages, Atf3 accumulated in the nucleus and chromatin-immunoprecipitation analysis showed that Atf3 is recruited to the Ptgs2 promoter region. In acute peritonitis and in cutaneous wounds, there was increased leukocyte accumulation and higher levels of prostaglandins (PGE2/PGD2) in inflammatory exudates of Atf3−/− mice compared with WT mice. Collectively, these results demonstrate that during acute inflammation Atf3 negatively regulates Ptgs2 and therefore dysregulation of this axis could potentially contribute to aberrant Ptgs2 expression in chronic inflammatory diseases. Moreover, this axis could be a new therapeutic target for suppressing Ptgs2 expression and the resultant inflammatory responses. PMID:25619459

  6. Process Damping Parameters

    NASA Astrophysics Data System (ADS)

    Turner, Sam

    2011-12-01

    The phenomenon of process damping as a stabilising effect in milling has been encountered by machinists since milling and turning began. It is of great importance when milling aerospace alloys where maximum surface speed is limited by excessive tool wear and high speed stability lobes cannot be attained. Much of the established research into regenerative chatter and chatter avoidance has focussed on stability lobe theory with different analytical and time domain models developed to expand on the theory first developed by Trusty and Tobias. Process damping is a stabilising effect that occurs when the surface speed is low relative to the dominant natural frequency of the system and has been less successfully modelled and understood. Process damping is believed to be influenced by the interference of the relief face of the cutting tool with the waveform traced on the cut surface, with material properties and the relief geometry of the tool believed to be key factors governing performance. This study combines experimental trials with Finite Element (FE) simulation in an attempt to identify and understand the key factors influencing process damping performance in titanium milling. Rake angle, relief angle and chip thickness are the variables considered experimentally with the FE study looking at average radial and tangential forces and surface compressive stress. For the experimental study a technique is developed to identify the critical process damping wavelength as a means of measuring process damping performance. For the range of parameters studied, chip thickness is found to be the dominant factor with maximum stable parameters increased by a factor of 17 in the best case. Within the range studied, relief angle was found to have a lesser effect than expected whilst rake angle had an influence.

  7. Note: Tesla transformer damping

    NASA Astrophysics Data System (ADS)

    Reed, J. L.

    2012-07-01

    Unexpected heavy damping in the two winding Tesla pulse transformer is shown to be due to small primary inductances. A small primary inductance is a necessary condition of operability, but is also a refractory inefficiency. A 30% performance loss is demonstrated using a typical "spiral strip" transformer. The loss is investigated by examining damping terms added to the transformer's governing equations. A significant alteration of the transformer's architecture is suggested to mitigate these losses. Experimental and simulated data comparing the 2 and 3 winding transformers are cited to support the suggestion.

  8. Damping seals for turbomachinery

    NASA Technical Reports Server (NTRS)

    Vonpragenau, G. L.

    1985-01-01

    Rotor whirl stabilization of high performance turbomachinery which operates at supercritical speed is discussed. Basic whirl driving forces are reviewed. Stabilization and criteria are discussed. Damping seals are offered as a solution to whirl and high vibration problems. Concept, advantages, retrofitting, and limits of damping seals are explained. Dynamic and leakage properties are shown to require a rough stator surface for stability and efficiency. Typical seal characteristics are given for the case of the high pressure oxidizer turbopump of the Space Shuttle. Ways of implementation and bearing load effects are discussed.

  9. Endoplasmic Reticulum Stress-Activated Transcription Factor ATF6α Requires the Disulfide Isomerase PDIA5 To Modulate Chemoresistance

    PubMed Central

    Higa, Arisa; Taouji, Said; Lhomond, Stéphanie; Jensen, Devon; Fernandez-Zapico, Martin E.; Simpson, Jeremy C.; Pasquet, Jean-Max; Schekman, Randy

    2014-01-01

    ATF6α, a membrane-anchored transcription factor from the endoplasmic reticulum (ER) that modulates the cellular response to stress as an effector of the unfolded-protein response (UPR), is a key player in the development of tumors of different origin. ATF6α activation has been linked to oncogenic transformation and tumor maintenance; however, the mechanism(s) underlying this phenomenon remains elusive. Here, using a phenotypic small interfering RNA (siRNA) screening, we identified a novel role for ATF6α in chemoresistance and defined the protein disulfide isomerase A5 (PDIA5) as necessary for ATF6α activation upon ER stress. PDIA5 contributed to disulfide bond rearrangement in ATF6α under stress conditions, thereby leading to ATF6α export from the ER and activation of its target genes. Further analysis of the mechanism demonstrated that PDIA5 promotes ATF6α packaging into coat protein complex II (COPII) vesicles and that the PDIA5/ATF6α activation loop is essential to confer chemoresistance on cancer cells. Genetic and pharmacological inhibition of the PDIA5/ATF6α axis restored sensitivity to the drug treatment. This work defines the mechanisms underlying the role of ATF6α activation in carcinogenesis and chemoresistance; furthermore, it identifies PDIA5 as a key regulator ATF6α-mediated cellular functions in cancer. PMID:24636989

  10. Regulation of Gγ-Globin Gene by ATF2 and Its Associated Proteins through the cAMP-Response Element

    PubMed Central

    Dhar, Ruby; Mahajan, Milind; Choudhury, Alina; Weissman, Sherman; Pace, Betty S.

    2013-01-01

    The upstream Gγ-globin cAMP-response element (G-CRE) plays an important role in regulating Gγ-globin expression through binding of ATF2 and its DNA-binding partners defined in this study. ATF2 knockdown resulted in a significant reduction of γ-globin expression accompanied by decreased ATF2 binding to the G-CRE. By contrast, stable ATF2 expression in K562 cells increased γ-globin transcription which was reduced by ATF2 knockdown. Moreover, a similar effect of ATF2 on γ-globin expression was observed in primary erythroid progenitors. To understand the role of ATF2 in γ-globin expression, chromatographically purified G-CRE/ATF2-interacting proteins were subjected to mass spectrometry analysis; major binding partners included CREB1, cJun, Brg1, and histone deacetylases among others. Immunoprecipitation assays demonstrated interaction of these proteins with ATF2 and in vivo GCRE binding in CD34+ cells undergoing erythroid differentiation which was correlated with γ-globin expression during development. These results suggest synergism between developmental stage-specific recruitments of the ATF2 protein complex and expression of γ-globin during erythropoiesis. Microarray studies in K562 cells support ATF2 plays diverse roles in hematopoiesis and chromatin remodeling. PMID:24223142

  11. The Brookhaven ATF low-emittance beam line

    SciTech Connect

    Wang, X.J. . Center for Advanced Accelerators Physics); Kirk, H.G. )

    1991-01-01

    One component of the experimental program at the Brookhaven Accelerator Test Facility (ATF) consists of a class of experiments which will study the acceleration of electrons through micron-size structures which are exposed in coincidence to a 100 GW CO{sub 2} laser beam. These experiments require the development and control of an electron beam with geometric emittances on the order of 10{sup {minus}10} m-rad and intensities on the order of 10{sup 6} electrons. In this paper, we describe the strategies for producing such beams and the effects of high-order aberrations. Particle tracking results are presented for the final-focus system. 9 refs., 6 figs., 2 tabs.

  12. Scenarios for the ATF2 Ultra-Low Betas Proposal

    SciTech Connect

    Marin, Eduardo; Tomas, Rogelio; Bambade, Philip; Kuroda, Shigeru; Okugi, Toshiyuki; Tauchi, Toshiaki; Terunuma, Nobuhiro; Urakawa, Junji; Parker, Brett; Seryi, Andrei; White, Glen; Woodley, Mark; /SLAC

    2012-06-29

    The current ATF2 Ultra-Low beta proposal was designed to achieve 20nm vertical IP beam size without considering the multipolar components of the FD magnets. In this paper we describe different scenarios that avoid the detrimental effect of these multipolar errors to the beam size at the interaction point (IP). The simplest approach consists in modifying the optics, but other solutions are studied as the introduction of super-conducting wigglers to reduce the emittance or the replacement of the normal-conducting focusing quadrupole in the Final Doublet (NC-QF1FF) with a super-conducting quadrupole one (SC-QF1FF). These are fully addressed in the paper.

  13. Scenarios For The ATF2 Ultra-Low Betas Proposal

    SciTech Connect

    Marin E.; Parker B.; Tomas R. Bambade Kuroda S. Okugi T. Tauchi T. Terunuma N. Urakawa J. Seryi A. White G. Woodley M.

    2010-05-23

    The current ATF2 Ultra-Low beta proposal was designed to achieve 20nm vertical IP beam size without considering the multipolar components of the FD magnets. In this paper we describe different scenarios that avoid the detrimental effect of these multipolar errors to the beam size at the interaction point (IP). The simplest approach consists in modifying the optics, but other solutions are studied as the introduction of super-conducting wigglers to reduce the emittance or the replacement of the normal-conducting focusing quadrupole in the Final Doublet (NC-QF1FF) with a super-conducting quadrupole one (SC-QF1FF). These are fully addressed in the paper.

  14. Planetary rings

    SciTech Connect

    Greenberg, R.; Brahic, A.

    1984-01-01

    Among the topics discussed are the development history of planetary ring research, the view of planetary rings in astronomy and cosmology over the period 1600-1900, the characteristics of the ring systems of Saturn and Uranus, the ethereal rings of Jupiter and Saturn, dust-magnetosphere interactions, the effects of radiation forces on dust particles, the collisional interactions and physical nature of ring particles, transport effects due to particle erosion mechanisms, and collision-induced transport processes in planetary rings. Also discussed are planetary ring waves, ring particle dynamics in resonances, the dynamics of narrow rings, the origin and evolution of planetary rings, the solar nebula and planetary disk, future studies of the planetary rings by space probes, ground-based observatories and earth-orbiting satellites, and unsolved problems in planetary ring dynamics.

  15. Rubber-coated bellows improves vibration damping in vacuum lines

    NASA Technical Reports Server (NTRS)

    Hegland, D. E.; Smith, R. J.

    1966-01-01

    Compact-vibration damping systems, consisting of rubber-coated metal bellows with a sliding O-ring connector, are used in vacuum lines. The device presents a metallic surface to the vacuum system and combines flexibility with the necessary stiffness. It protects against physical damage, reduces fatigue failure, and provides easy mating of nonparallel lines.

  16. Dynamical and photometric studies of Saturn's rings

    SciTech Connect

    Dones, H.C. Jr.

    1987-01-01

    Imaging and occultation data returned by the two Voyager spacecraft have deepened understanding of dynamical processes operating in Saturn's rings. Saturn's outermost, or A, ring was investigated here in light of this data. Most structure seen in the A ring is caused by spiral density waves, which are regular oscillations in the optical depth of the rings. Density waves are excited at horizontal resonances with the inner satellites of Saturn. Most waves are weakly nonlinear and propagate for only a few wavelengths before they damp. The damping length is somewhat longer in regions of high optical depth. A theory of the viscous damping of nonlinear density waves in the rings is formulated. The energy input by the wave must be offset, in a steady state, by dissipation through inelastic collisions between ring particles. Voyage images of the A ring are analyzed to determine how the brightness of different regions varies with illumination and viewing geometry. The data are modeled with a radiative-transfer code that includes the illumination of the rings by the Sun and Saturn. Finally, the azimuthally asymmetric reflectivity of the A ring seen in Voyager images taken in backscatter is characterized.

  17. Recent electron temperature and density results from the ATF Thomson scattering system

    SciTech Connect

    Rasmussen, D.A.; England, A.C.; Murakami, M.; Howe, H.C.; Clark, T.L.; Kindsfather, R.R.; Rayburn, T.M.; Stewart, K.A.; Rogers, P.S.; Bell, G.L.

    1989-01-01

    A spatial multipoint Thomson scattering system has been developed for the Advanced Toroidal Facility (ATF) torsatron. The system measures temperature and density at 15 vertical locations on a vertical chord for each laser shot (one per plasma discharge). By remotely relocating the laser beam and reconfiguring the viewing optics during a series of ATF discharges, a two-dimensional (2-D) electron temperature and density map of the plasma cross section can be obtained. Results obtained with this system during ATF operation in 1988 and early 1989 are presented. 7 refs., 6 figs.

  18. ATF CO{sub 2} laser system upgrade to terawatt peak power

    SciTech Connect

    Pogorelsky, I.V.

    1995-05-01

    This document describes the proposed upgrade of the 10-GW peak power 50-ps CO{sub 2} laser presently operational at the ATF to the 1 TW level at a shorter, 3--10 ps, pulse duration. The approach adopted is based on state of the art CO{sub 2} laser technology and an experience gained in the course of the ATF laser design and application for the laser accelerator experiment. The proposed upgrade is an economical way for the ATF to become in a short time among leading users facilities available for next generation ({ge} 100 MeV) laser accelerator studies.

  19. Specifications of the octupole magnets required for the ATF2 ultra-low ß* lattice

    SciTech Connect

    Marin, E.; Modena, M.; Tauchi, T.; Terunuma, N.; Tomas, R.; White, G.R.; /SLAC

    2014-05-28

    The Accelerator Test Facility 2 (ATF2) aims to test the novel chromaticity correction for higher chromaticity lattices as the one of CLIC. To this end the ATF2 ultra-low ß* lattice is designed to vertically focus the beam at the focal point or usually referred to as interaction point (IP), down to 23 nm. However when the measured multipole components of the ATF2 magnets are considered in the simulations, the evaluated spot sizes at the IP are well above the design value. The designed spot size is effectively recovered by inserting a pair of octupole magnets. In this note we address the technical specifications required for these octupole magnets.

  20. ATF3 Protects Pulmonary Resident Cells from Acute and Ventilator-Induced Lung Injury by Preventing Nrf2 Degradation

    PubMed Central

    Shan, Yuexin; Akram, Ali; Amatullah, Hajera; Zhou, Dun Yuan; Gali, Patricia L.; Maron-Gutierrez, Tatiana; González-López, Adrian; Zhou, Louis; Rocco, Patricia R.M.; Hwang, David; Albaiceta, Guillermo M.; Haitsma, Jack J.

    2015-01-01

    Abstract Aims: Ventilator-induced lung injury (VILI) contributes to mortality in patients with acute respiratory distress syndrome, the most severe form of acute lung injury (ALI). Absence of activating transcription factor 3 (ATF3) confers susceptibility to ALI/VILI. To identify cell-specific ATF3-dependent mechanisms of susceptibility to ALI/VILI, we generated ATF3 chimera by adoptive bone marrow (BM) transfer and randomized to inhaled saline or lipopolysacharide (LPS) in the presence of mechanical ventilation (MV). Adenovirus vectors to silence or overexpress ATF3 were used in primary human bronchial epithelial cells and murine BM-derived macrophages from wild-type or ATF3-deficient mice. Results: Absence of ATF3 in myeloid-derived cells caused increased pulmonary cellular infiltration. In contrast, absence of ATF3 in parenchymal cells resulted in loss of alveolar-capillary membrane integrity and increased exudative edema. ATF3-deficient macrophages were unable to limit the expression of pro-inflammatory mediators. Knockdown of ATF3 in resident cells resulted in decreased junctional protein expression and increased paracellular leak. ATF3 overexpression abrogated LPS induced membrane permeability. Despite release of ATF3-dependent Nrf2 transcriptional inhibition, mice that lacked ATF3 expression in resident cells had increased Nrf2 protein degradation. Innovation: In our model, in the absence of ATF3 in parenchymal cells increased Nrf2 degradation is the result of increased Keap-1 expression and loss of DJ-1 (Parkinson disease [autosomal recessive, early onset] 7), previously not known to play a role in lung injury. Conclusion: Results suggest that ATF3 confers protection to lung injury by preventing inflammatory cell recruitment and barrier disruption in a cell-specific manner, opening novel opportunities for cell specific therapy for ALI/VILI. Antioxid. Redox Signal. 22, 651–668. PMID:25401197

  1. p53 and ATF4 mediate distinct and additive pathways to skeletal muscle atrophy during limb immobilization

    PubMed Central

    Fox, Daniel K.; Ebert, Scott M.; Bongers, Kale S.; Dyle, Michael C.; Bullard, Steven A.; Dierdorff, Jason M.; Kunkel, Steven D.

    2014-01-01

    Immobilization causes skeletal muscle atrophy via complex signaling pathways that are not well understood. To better understand these pathways, we investigated the roles of p53 and ATF4, two transcription factors that mediate adaptations to a variety of cellular stresses. Using mouse models, we demonstrate that 3 days of muscle immobilization induces muscle atrophy and increases expression of p53 and ATF4. Furthermore, muscle fibers lacking p53 or ATF4 are partially resistant to immobilization-induced muscle atrophy, and forced expression of p53 or ATF4 induces muscle fiber atrophy in the absence of immobilization. Importantly, however, p53 and ATF4 do not require each other to promote atrophy, and coexpression of p53 and ATF4 induces more atrophy than either transcription factor alone. Moreover, muscle fibers lacking both p53 and ATF4 are more resistant to immobilization-induced atrophy than fibers lacking only p53 or ATF4. Interestingly, the independent and additive nature of the p53 and ATF4 pathways allows for combinatorial control of at least one downstream effector, p21. Using genome-wide mRNA expression arrays, we identified p21 mRNA as a skeletal muscle transcript that is highly induced in immobilized muscle via the combined actions of p53 and ATF4. Additionally, in mouse muscle, p21 induces atrophy in a manner that does not require immobilization, p53 or ATF4, and p21 is required for atrophy induced by immobilization, p53, and ATF4. Collectively, these results identify p53 and ATF4 as essential and complementary mediators of immobilization-induced muscle atrophy and discover p21 as a critical downstream effector of the p53 and ATF4 pathways. PMID:24895282

  2. Placental endoplasmic reticulum stress negatively regulates transcription of placental growth factor via ATF4 and ATF6β: implications for the pathophysiology of human pregnancy complications

    PubMed Central

    Mizuuchi, Masahito; Cindrova‐Davies, Tereza; Olovsson, Matts; Charnock‐Jones, D Stephen; Burton, Graham J

    2016-01-01

    Abstract Low maternal circulating concentrations of placental growth factor (PlGF) are one of the hallmarks of human pregnancy complications, including fetal growth restriction (FGR) and early‐onset pre‐eclampsia (PE). Currently, PlGF is used clinically with other biomarkers to screen for high‐risk cases, although the mechanisms underlying its regulation are largely unknown. Placental endoplasmic reticulum (ER) stress has recently been found to be elevated in cases of FGR, and to an even greater extent in early‐onset PE complicated with FGR. ER stress activates the unfolded protein response (UPR); attenuation of protein translation and a reduction in cell growth and proliferation play crucial roles in the pathophysiology of these complications of pregnancy. In this study, we further identified that ER stress regulates release of PlGF. We first observed that down‐regulation of PlGF protein was associated with nuclear localization of ATF4, ATF6α and ATF6β in the syncytiotrophoblast of placentae from PE patients. Transcript analysis showed a decrease of PlGF mRNA, and an increase from genes encoding those UPR transcription factors in placentae from cases of early‐onset PE, but not of late‐onset (>34 weeks) PE, compared to term controls. Further investigations indicated a strong correlation between ATF4 and PlGF mRNA levels only (r = − 0.73, p < 0.05). These results could be recapitulated in trophoblast‐like cells exposed to chemical inducers of ER stress or hypoxia–reoxygenation. The stability of PlGF transcripts was unchanged. The use of small interfering RNA specific for transcription factors in the UPR pathways revealed that ATF4 and ATF6β, but not ATF6α, modulate PlGF transcription. To conclude, ATF4 and ATF6β act synergistically in the negative regulation of PlGF mRNA expression, resulting in reduced PlGF secretion by the trophoblast in response to stress. Therefore, these results further support the targeting of placental ER stress as a

  3. Damping seal for turbomachinery

    NASA Technical Reports Server (NTRS)

    Vonpragenau, G. L. (Inventor)

    1985-01-01

    A damping seal between a high speed rotor member and stator member that separates pressurized fluid compartments is described. It is characterized by the rotor member having a smooth outer surface and the stator member having its bore surface roughened by a plurality of pockets or depressions.

  4. Damping formulas and experimental values of damping in flutter models

    NASA Technical Reports Server (NTRS)

    Coleman, Robert P

    1940-01-01

    The problem of determining values of structural damping for use in flutter calculations is discussed. The concept of equivalent viscous damping is reviewed and its relation to the structural damping coefficient g introduced in NACA Technical Report No. 685 is shown. The theory of normal modes is reviewed and a number of methods are described for separating the motions associated with different modes. Equations are developed for use in evaluating the damping parameters from experimental data. Experimental results of measurements of damping in several flutter models are presented.

  5. Divest yourself of a preconceived idea: transcription factor ATF6 is not a soluble protein!

    PubMed

    Mori, Kazutoshi

    2010-05-01

    The unfolded protein response (UPR), an evolutionarily conserved transcriptional induction program that is coupled with intracellular signaling from the endoplasmic reticulum (ER) to the nucleus, is activated to cope with ER stress and to maintain the homeostasis of the ER. In 1996, we isolated a basic leucine zipper protein, which had been previously named activating transcription factor (ATF)6, as a candidate transcription factor responsible for the mammalian UPR. Subsequent analysis, however, was confounding. The problem was eventually tracked down to an unusual property of ATF6: rather than being a soluble nuclear protein, as expected for an active transcription factor, ATF6 was instead synthesized as a transmembrane protein embedded in the ER, which was activated by ER stress-induced proteolysis. ATF6 was thus unique: an ER stress sensor/transducer that is involved in all steps of the UPR, from the sensing step in the ER to the transcriptional activation step in the nucleus. PMID:20219975

  6. Divest Yourself of a Preconceived Idea: Transcription Factor ATF6 Is Not a Soluble Protein!

    PubMed Central

    2010-01-01

    The unfolded protein response (UPR), an evolutionarily conserved transcriptional induction program that is coupled with intracellular signaling from the endoplasmic reticulum (ER) to the nucleus, is activated to cope with ER stress and to maintain the homeostasis of the ER. In 1996, we isolated a basic leucine zipper protein, which had been previously named activating transcription factor (ATF)6, as a candidate transcription factor responsible for the mammalian UPR. Subsequent analysis, however, was confounding. The problem was eventually tracked down to an unusual property of ATF6: rather than being a soluble nuclear protein, as expected for an active transcription factor, ATF6 was instead synthesized as a transmembrane protein embedded in the ER, which was activated by ER stress-induced proteolysis. ATF6 was thus unique: an ER stress sensor/transducer that is involved in all steps of the UPR, from the sensing step in the ER to the transcriptional activation step in the nucleus. PMID:20219975

  7. Saturn's rings

    NASA Technical Reports Server (NTRS)

    2000-01-01

    When seen from the unlit side, the rings of Saturn present a much different appearance from that familiar to telescopic observers. Relatively opaque areas like the B Ring turn black, while lightly populated zones, such as the C Ring and the Cassini Division, prove to excellent diffuse transmitters of sunlight. The A Ring, with intermediate opacity, is at an intermediate level of brightness.

  8. ATF3 Mediates Anti-Cancer Activity of Trans-10, cis-12-Conjugated Linoleic Acid in Human Colon Cancer Cells

    PubMed Central

    Kim, Kui-Jin; Lee, Jihye; Park, Yeonhwa; Lee, Seong-Ho

    2015-01-01

    Conjugated linoleic acids (CLA) are a family of isomers of linoleic acid. CLA increases growth arrest and apoptosis of human colorectal cancer cells through an isomer-specific manner. ATF3 belongs to the ATF/CREB family of transcription factors and is associated with apoptosis in colorectal cancer. The present study was performed to investigate the molecular mechanism by which t10, c12-CLA stimulates ATF3 expression and apoptosis in human colorectal cancer cells. t10, c12-CLA increased an apoptosis in human colorectal cancer cells in dose dependent manner. t10, c12-CLA induced ATF3 mRNA and luciferase activity of ATF3 promoter in a dose-dependent manner. The responsible region for ATF3 transcriptional activation by t10, c12-CLA is located between −147 and −1850 of ATF3 promoter. mRNA stability of ATF3 was not affected by t10, c12-CLA treatment. t10, c12-CLA increases GSK3β expression and suppresses IGF-1-stimulated phosphorylation of Akt. The knockdown of ATF3 suppressed expression of GSK3β and NAG-1 and PARP cleavage. The results suggest that t10, c12-CLA induces apoptosis through ATF3-mediated pathway in human colorectal cancer cells. PMID:25767681

  9. Development of Electronics for the ATF2 Interaction Point Region Beam Position Monitor

    SciTech Connect

    Kim, Youngim; Heo, Ae-young; Kim, Eun-San; Boogert, Stewart; Honda, Yosuke; Tauchi, Toshiaki; Terunuma, Nobuhiro; May, Justin; McCormick, Douglas; Smith, Tonee; /SLAC

    2012-08-14

    Nanometer resolution beam position monitors have been developed to measure and control beam position stability at the interaction point region of ATF2. The position of the beam has to be measured to within a few nanometers at the interaction point. In order to achieve this performance, electronics for the low-Q IP-BPM was developed. Every component of the electronics have been simulated and checked on the bench and using the ATF2 beam. We will explain each component and define their working range. Then, we will show the performance of the electronics measured with beam signal. ATF2 is a final focus test beam line for ILC in the framework of the ATF international collaboration. The new beam line was constructed to extend the extraction line at ATF, KEK, Japan. The first goal of ATF2 is the acheiving of a 37 nm vertical beam size at focal point (IP). The second goal is to stabilize the beam at the focal point at a few nanometer level for a long period in order to ensure the high luminosity. To achieve these goals a high resolution IP-BPM is essential. In addition for feedback applications a low-Q system is desirable.

  10. FoxO1 Protein Cooperates with ATF4 Protein in Osteoblasts to Control Glucose Homeostasis*

    PubMed Central

    Kode, Aruna; Mosialou, Ioanna; Silva, Barbara C.; Joshi, Sneha; Ferron, Mathieu; Rached, Marie Therese; Kousteni, Stavroula

    2012-01-01

    The Forkhead transcription factor FoxO1 inhibits through its expression in osteoblasts β-cell proliferation, insulin secretion, and sensitivity. At least part of the FoxO1 metabolic functions result from its ability to suppress the activity of osteocalcin, an osteoblast-derived hormone favoring glucose metabolism and energy expenditure. In searching for mechanisms mediating the metabolic actions of FoxO1, we focused on ATF4, because this transcription factor also affects glucose metabolism through its expression in osteoblasts. We show here that FoxO1 co-localizes with ATF4 in the osteoblast nucleus, and physically interacts with and promotes the transcriptional activity of ATF4. Genetic experiments demonstrate that FoxO1 and ATF4 cooperate to increase glucose levels and decrease glucose tolerance. These effects result from a synergistic effect of the two transcription factors to suppress the activity of osteocalcin through up-regulating expression of the phosphatase catalyzing osteocalcin inactivation. As a result, insulin production by β-cells and insulin signaling in the muscle, liver and white adipose tissue are compromised and fat weight increases by the FoxO1/ATF4 interaction. Taken together these observations demonstrate that FoxO1 and ATF4 cooperate in osteoblasts to regulate glucose homeostasis. PMID:22298775

  11. ATF-2 immunoreactivity in post-mitotic and terminally differentiated human odontoblasts.

    PubMed

    Keklikoglu, Nurullah; Akinci, Sevtap

    2015-09-01

    Activating transcription factor 2 (ATF-2/CRE-BP1; cAMP-responsive element binding protein 1) is a member of nuclear transcription factor activator protein-1 (AP-1) family. AP-1 regulates cellular processes including growth, proliferation, differentiation and apoptosis. However, biological relationship of cellular process to each member of the AP-1 family is not clear yet. The objective of the present study was to compare the ATF-2 immunoreactivity in the post-mitotic and terminally differentiated odontoblasts and in the pulpal fibroblasts which can be divided by mitosis when required. Fibroblasts at various stages of differentiation co-exist in the human dental pulp. ATF-2 was investigated immunohistochemically in 20 permanent human teeth. According to the findings obtained, the mean percentage of ATF-2 positive cells was 68.5 ± 19.2% in the odontoblasts and 22.8 ± 13.7% in the pulpal fibroblasts. The comparison of ATF-2 positivity revealed a statistically significant difference between odontoblasts and pulpal fibroblasts. These findings have suggested that ATF-2 is more associated with cell survival rather than cell proliferation, and revealed much of effectiveness in maintaining terminal differentiation than the various differentiation stages of the cells. PMID:25417007

  12. Damping seals for turbomachinery

    NASA Technical Reports Server (NTRS)

    Vonpragenau, G. L.

    1982-01-01

    A rotor seal is proposed that restricts leakage like a labyrinth seal, but extends the stabilizing speed range beyond twice the first critical speed. The dynamic parameters were derived from bulk flow equations without requiring a dominant axial flow. The flow is considered incompressible and turbulent. Damping seals are shown to be feasible for extending the speed range of high performance turbomachinery beyond the limit imposed by conventional seals.

  13. Experiment on suppression of spontaneous undulator radiation at ATF

    SciTech Connect

    Litvinenko,V.; Yakimenko, V.

    2009-08-23

    We propose undertaking a demonstration experiment on suppressing spontaneous undulator radiation from an electron beam at BNL's Accelerator Test Facility (ATF). We describe the method, the proposed layout, and a possible schedule. There are several advantages in strongly suppressing shot noise in the electron beam, and the corresponding spontaneous radiation. The self-amplified spontaneous (SASE) emission originating from shot noise in the electron beam is the main source of noise in high-gain FEL amplifiers. It may negatively affect several HG FEL applications ranging from single- to multi-stage HGHG FELs. SASE saturation also imposes a fundamental hard limit on the gain of an FEL amplifier in a coherent electron-cooling scheme. A novel active method for suppressing shot noise in relativistic electron beams by many orders-of-magnitude was recently proposed. While theoretically such strong suppression appears feasible, the performance and applicability of this novel method must be evaluated experimentally. Several practical questions about the proposed noise suppressor, such as 3D effects and/or sensitivity to the e-beam parameters also require experimental clarification. To do this, we propose here a proof-of-principle experiment using elements of the VISA FEL at BNL's Accelerator Test Facility.

  14. Saturn's Rings

    NASA Astrophysics Data System (ADS)

    Cuzzi, J. N.

    2014-12-01

    The rings are changing before our eyes; structure varies on all timescales and unexpected things have been discovered. Many questions have been answered, but some answers remain elusive (see Cuzzi et al 2010 for a review). Here we highlight the major ring science progress over the mission to date, and describe new observations planned for Cassini's final three years. Ring Composition and particle sizes: The rings are nearly all water ice with no other ices - so why are they reddish? The C Ring and Cassini Division are "dirtier" than the more massive B and A Rings, as shown by near-IR and, recently, microwave observations. Particle sizes, from stellar and radio occultations, vary from place to place. Ring structure, micro and macro: numerous spiral density waves and ubiquitous "self-gravity wakes" reveal processes which fostered planet formation in the solar system and elsewhere. However, big puzzles remain regarding the main ring divisions, the C Ring plateau structures, and the B Ring irregular structure. Moonlets, inside and out, seen and unseen: Two gaps contain sizeable moonlets, but more gaps seem to contain none; even smaller embedded "propeller" objects wander, systematically or randomly, through the A ring. Rubble pile ringmoons just outside the rings may escaped from the rings, and the recently discovered "Peggy" may be trying this as we watch. Impact bombardment of the rings: Comet fragments set the rings to rippling on century-timescales, and boulders crash through hourly; meanwhile, the constant hail of infalling Kuiper belt material has a lower mass flux than previously thought. Origin and Age of the Rings: The ring mass and bombardment play key roles. The ring mass is well known everywhere but in the B Ring (where most of it is). New models suggest how tidal breakup of evolving moons may have formed massive ancient rings, of which the current ring is just a shadow. During its last three years, the Cassini tour profile will allow entirely new

  15. Methods for improving damping. Part 3: Damping material data

    NASA Astrophysics Data System (ADS)

    1992-12-01

    ESDU 92001 presents modulus and loss factor for 27 damping materials in the form of reduced temperature nomograms which allow the effects of frequency and temperature on those properties to be considered simultaneously. The data were supplied by 5 manufacturers, and their addresses in the UK and US are provided. The information is a necessary input in computational procedures, described in ESDU 91013, dealing with the application of layered damping treatments to beam- and plate-like structures. Notes are included on the measurement and reliability of the damping quantities that affect the variability allowances to be made when assessing a damping treatment for a particular application. Factors to be considered when selecting a damping material are discussed. Approximate relationships between the elastic properties of damping materials are given.

  16. Hepatic ATF6 Increases Fatty Acid Oxidation to Attenuate Hepatic Steatosis in Mice Through Peroxisome Proliferator-Activated Receptor α.

    PubMed

    Chen, Xuqing; Zhang, Feifei; Gong, Qi; Cui, Aoyuan; Zhuo, Shu; Hu, Zhimin; Han, Yamei; Gao, Jing; Sun, Yixuan; Liu, Zhengshuai; Yang, Zhongnan; Le, Yingying; Gao, Xianfu; Dong, Lily Q; Gao, Xin; Li, Yu

    2016-07-01

    The endoplasmic reticulum quality control protein activating transcription factor 6 (ATF6) has emerged as a novel metabolic regulator. Here, we show that adenovirus-mediated overexpression of the dominant-negative form of ATF6 (dnATF6) increases susceptibility to develop hepatic steatosis in diet-induced insulin-resistant mice and fasted mice. Overexpression of dnATF6 or small interfering RNA-mediated knockdown of ATF6 decreases the transcriptional activity of peroxisome proliferator-activated receptor α (PPARα)/retinoid X receptor complex, and inhibits oxygen consumption rates in hepatocytes, possibly through inhibition of the binding of PPARα to the promoter of its target gene. Intriguingly, ATF6 physically interacts with PPARα, enhances the transcriptional activity of PPARα, and triggers activation of PPARα downstream targets, such as CPT1α and MCAD, in hepatocytes. Furthermore, hepatic overexpression of the active form of ATF6 promotes hepatic fatty acid oxidation and protects against hepatic steatosis in diet-induced insulin-resistant mice. These data delineate the mechanism by which ATF6 controls the activity of PPARα and hepatic mitochondria fatty acid oxidation. Therefore, strategies to activate ATF6 could be used as an alternative avenue to improve liver function and treat hepatic steatosis in obesity. PMID:27207533

  17. Vascular ring

    MedlinePlus

    ... with aberrant subclavian and left ligamentum ateriosus; Congenital heart defect - vascular ring; Birth defect heart - vascular ring ... accounts for less than 1% of all congenital heart problems. The condition occurs as often in males ...

  18. Neptune's rings

    NASA Technical Reports Server (NTRS)

    1999-01-01

    This 591-second exposure of the rings of Neptune were taken with the clear filter by the Voyager 2 wide-angle camera. The two main rings are clearly visible and appear complete over the region imaged. Also visible in this image is the inner faint ring and the faint band which extends smoothly from the ring roughly halfway between the two bright rings. Both of these newly discovered rings are broad and much fainter than the two narrow rings. The bright glare is due to over-exposure of the crescent on Neptune. Numerous bright stars are evident in the background. Both bright rings have material throughout their entire orbit, and are therefore continuous. The Voyager Mission is conducted by JPL for NASA's Office of Space Science and Applications.

  19. On damping mechanisms in beams

    NASA Technical Reports Server (NTRS)

    Banks, H. T.; Inman, D. J.

    1989-01-01

    A partial differential equation model of a cantilevered beam with a tip mass at its free end is used to study damping in a composite. Four separate damping mechanisms consisting of air damping, strain rate damping, spatial hysteresis and time hysteresis are considered experimentally. Dynamic tests were performed to produce time histories. The time history data is then used along with an approximate model to form a sequence of least squares problems. The solution of the least squares problem yields the estimated damping coefficients. The resulting experimentally determined analytical model is compared with the time histories via numerical simulation of the dynamic response. The procedure suggested here is compared with a standard modal damping ratio model commonly used in experimental modal analysis.

  20. Damping measurements in flowing water

    NASA Astrophysics Data System (ADS)

    Coutu, A.; Seeley, C.; Monette, C.; Nennemann, B.; Marmont, H.

    2012-11-01

    Fluid-structure interaction (FSI), in the form of mass loading and damping, governs the dynamic response of water turbines, such as Francis turbines. Water added mass and damping are both critical quantities in evaluating the dynamic response of the turbine component. Although the effect of fluid added mass is well documented, fluid damping, a critical quantity to limit vibration amplitudes during service, and therefore to help avoiding possible failure of the turbines, has received much less attention in the literature. This paper presents an experimental investigation of damping due to FSI. The experimental setup, designed to create dynamic characteristics similar to the ones of Francis turbine blades is discussed, together with the experimental protocol and examples of measurements obtained. The paper concludes with the calculated damping values and a discussion on the impact of the observed damping behaviour on the response of hydraulic turbine blades to FSI.

  1. Magnetically Damped Furnace (MDF)

    NASA Technical Reports Server (NTRS)

    1998-01-01

    The Magnetically Damped Furnace (MDF) breadboard is being developed in response to NASA's mission and goals to advance the scientific knowledge of microgravity research, materials science, and related technologies. The objective of the MDF is to dampen the fluid flows due to density gradients and surface tension gradients in conductive melts by introducing a magnetic field during the sample processing. The MDF breadboard will serve as a proof of concept that the MDF performance requirements can be attained within the International Space Station resource constraints.

  2. The Joint Damping Experiment (JDX)

    NASA Technical Reports Server (NTRS)

    Folkman, Steven L.; Bingham, Jeff G.; Crookston, Jess R.; Dutson, Joseph D.; Ferney, Brook D.; Ferney, Greg D.; Rowsell, Edwin A.

    1997-01-01

    The Joint Damping Experiment (JDX), flown on the Shuttle STS-69 Mission, is designed to measure the influence of gravity on the structural damping of a high precision three bay truss. Principal objectives are: (1) Measure vibration damping of a small-scale, pinjointed truss to determine how pin gaps give rise to gravity-dependent damping rates; (2) Evaluate the applicability of ground and low-g aircraft tests for predicting on-orbit behavior; and (3) Evaluate the ability of current nonlinear finite element codes to model the dynamic behavior of the truss. Damping of the truss was inferred from 'Twang' tests that involve plucking the truss structure and recording the decay of the oscillations. Results are summarized as follows. (1) Damping, rates can change by a factor of 3 to 8 through changing the truss orientation; (2) The addition of a few pinned joints to a truss structure can increase the damping by a factor as high as 30; (3) Damping is amplitude dependent; (4) As gravity induced preloads become large (truss long axis perpendicular to gravity vector) the damping is similar to non-pinjointed truss; (5) Impacting in joints drives higher modes in structure; (6) The torsion mode disappears if gravity induced preloads are low.

  3. The DAMPE mission

    NASA Astrophysics Data System (ADS)

    Wu, Jian; Guo, Jianhua; Chang, Jin; Cai, Mingsheng

    2016-07-01

    The DArk Matter Particle Explorer (DAMPE) was launched into space on Dec.17, 2015 to a 500km dawn-to-dusk sun-synchronous orbit aiming at detecting high energy electron(gamma) as well as cosmic heavy ions up to 10TeV and 1PeV respectively to try to understand the mechanisms of particle acceleration in celestial sources and the propagation of cosmic rays in the Galaxy, to probe the nature of dark matter, a form of matter necessary to account for gravitational effects observed in very large scale structures such as anomalies in the rotation of galaxies and the gravitational lensing of light by galaxy clusters that cannot be accounted for by the quantity of observed matter , and to study the high-energy behavior of gamma-ray bursts, pulsars, Active Galaxy Nuclei and other transients,etc. After months' commissioning, DAMPE has been in the observational mode. This paper reports the status of its detectors and latest results collected so far.

  4. The transcription factor ATF2 promotes melanoma metastasis by suppressing protein fucosylation

    PubMed Central

    Lau, Eric; Feng, Yongmei; Claps, Giuseppina; Fukuda, Michiko N.; Perlina, Ally; Donn, Dylan; Jilaveanu, Lucia; Kluger, Harriet; Freeze, Hudson H.; Ronai, Ze’ev A.

    2016-01-01

    Melanoma is one of the most lethal skin cancers worldwide, primarily because of its propensity to metastasize. Thus, the elucidation of mechanisms that govern metastatic propensity is urgently needed. We found that protein kinase Cε (PKCε)–mediated activation of activating transcription factor 2 (ATF2) controls the migratory and invasive behaviors of melanoma cells. PKCε-dependent phosphorylation of ATF2 promoted its transcriptional repression of the gene encoding fucokinase (FUK), which mediates the fucose salvage pathway and thus global cellular protein fucosylation. In primary melanocytes and cell lines representing early-stage melanoma, the abundance of PKCε-phosphorylated ATF2 was low, thereby enabling the expression of FUK and cellular protein fucosylation, which promoted cellular adhesion and reduced motility. In contrast, increased expression of the gene encoding PKCε and abundance of phosphorylated, transcriptionally active ATF2 were observed in advanced-stage melanomas and correlated with decreased FUK expression, decreased cellular protein fucosylation, attenuated cell adhesion, and increased cell motility. Restoring fucosylation in mice either by dietary fucose supplementation or by genetic manipulation of murine Fuk expression attenuated primary melanoma growth, increased the number of intratumoral natural killer cells, and decreased distal metastasis in murine isograft models. Tumor microarray analysis of human melanoma specimens confirmed reduced fucosylation in metastatic tumors and a better prognosis for primary melanomas that had high abundance of fucosylation. Thus, inhibiting PKCε or ATF2 or increasing protein fucosylation in tumor cells may improve clinical outcome in melanoma patients. PMID:26645581

  5. Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.

    PubMed

    Kohl, Susanne; Zobor, Ditta; Chiang, Wei-Chieh; Weisschuh, Nicole; Staller, Jennifer; Gonzalez Menendez, Irene; Chang, Stanley; Beck, Susanne C; Garcia Garrido, Marina; Sothilingam, Vithiyanjali; Seeliger, Mathias W; Stanzial, Franco; Benedicenti, Francesco; Inzana, Francesca; Héon, Elise; Vincent, Ajoy; Beis, Jill; Strom, Tim M; Rudolph, Günther; Roosing, Susanne; Hollander, Anneke I den; Cremers, Frans P M; Lopez, Irma; Ren, Huanan; Moore, Anthony T; Webster, Andrew R; Michaelides, Michel; Koenekoop, Robert K; Zrenner, Eberhart; Kaufman, Randal J; Tsang, Stephen H; Wissinger, Bernd; Lin, Jonathan H

    2015-07-01

    Achromatopsia (ACHM) is an autosomal recessive disorder characterized by color blindness, photophobia, nystagmus and severely reduced visual acuity. Using homozygosity mapping and whole-exome and candidate gene sequencing, we identified ten families carrying six homozygous and two compound-heterozygous mutations in the ATF6 gene (encoding activating transcription factor 6A), a key regulator of the unfolded protein response (UPR) and cellular endoplasmic reticulum (ER) homeostasis. Patients had evidence of foveal hypoplasia and disruption of the cone photoreceptor layer. The ACHM-associated ATF6 mutations attenuate ATF6 transcriptional activity in response to ER stress. Atf6(-/-) mice have normal retinal morphology and function at a young age but develop rod and cone dysfunction with increasing age. This new ACHM-related gene suggests a crucial and unexpected role for ATF6A in human foveal development and cone function and adds to the list of genes that, despite ubiquitous expression, when mutated can result in an isolated retinal photoreceptor phenotype. PMID:26029869

  6. Peripheral and central neuronal ATF3 precedes CD4+ T-cell infiltration in EAE.

    PubMed

    Frezel, Noémie; Sohet, Fabien; Daneman, Richard; Basbaum, Allan I; Braz, Joao M

    2016-09-01

    Experimental allergic encephalomyelitis (EAE), an animal model of multiple sclerosis produced by immunization with myelin oligodendrocyte glycoprotein (MOG) and adjuvants, results from profound T-cell mediated CNS demyelination. EAE is characterized by progressive, ascending motor dysfunction and symptoms of ongoing pain and hypersensitivity, in some cases preceding or concomitant with the motor deficits. In this regard, the EAE model mimics major features of multiple sclerosis, where a central neuropathic pain state is common. Although the latter condition is presumed to arise from a CNS loss of inhibitory controls secondary to the demyelination, dysfunction of sensory neurons may also contribute. Based on our previous studies that demonstrated the utility of monitoring expression of activating transcription factor 3 (ATF3), a sensitive marker of injured sensory neurons, here we followed both ATF3 and CD4+ T cells invasion of sensory ganglia (as well as the CNS) at different stages of the EAE model. We found that ATF3 is induced in peripheral sensory ganglia and brainstem well before the appearance of motor deficits. Unexpectedly, the ATF3 induction always preceded T cell infiltration, typically in adjacent, but non-overlapping regions. Surprisingly, control administration of the pertussis toxin and/or Complete Freund's adjuvants, without MOG, induced ATF3 in sensory neurons. In contrast, T cell infiltration only occurred with MOG. Taken together, our results suggest that the clinical manifestations in the EAE result not only from central demyelination but also from neuronal stress and subsequent pathophysiology of sensory neurons. PMID:27343802

  7. Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia

    PubMed Central

    Kohl, Susanne; Zobor, Ditta; Chiang, Wei-Chieh; Weisschuh, Nicole; Staller, Jennifer; Menendez, Irene Gonzalez; Chang, Stanley; Beck, Susanne C; Garrido, Marina Garcia; Sothilingam, Vithiyanjali; Seeliger, Mathias W; Stanzial, Franco; Benedicenti, Francesco; Inzana, Francesca; Héon, Elise; Vincent, Ajoy; Beis, Jill; Strom, Tim M; Rudolph, Günther; Roosing, Susanne; den Hollander, Anneke I; Cremers, Frans P M; Lopez, Irma; Ren, Huanan; Moore, Anthony T; Webster, Andrew R; Michaelides, Michel; Koenekoop, Robert K; Zrenner, Eberhart; Kaufman, Randal J; Tsang, Stephen H; Wissinger, Bernd; Lin, Jonathan H

    2015-01-01

    Achromatopsia (ACHM) is an autosomal recessive disorder characterized by color blindness, photophobia, nystagmus and severely reduced visual acuity. Using homozygosity mapping and whole-exome and candidate gene sequencing, we identified ten families carrying six homozygous and two compound-heterozygous mutations in the ATF6 gene (encoding activating transcription factor 6A), a key regulator of the unfolded protein response (UPR) and cellular endoplasmic reticulum (ER) homeostasis. Patients had evidence of foveal hypoplasia and disruption of the cone photoreceptor layer. The ACHM-associated ATF6 mutations attenuate ATF6 transcriptional activity in response to ER stress. Atf6−/− mice have normal retinal morphology and function at a young age but develop rod and cone dysfunction with increasing age. This new ACHM-related gene suggests a crucial and unexpected role for ATF6A in human foveal development and cone function and adds to the list of genes that, despite ubiquitous expression, when mutated can result in an isolated retinal photoreceptor phenotype. PMID:26029869

  8. Atorvastatin up-regulates TRIB3 independent of ATF4-CHOP pathway in atherosclerotic patients

    PubMed Central

    Bi, Shao-Jie; Wang, Chun-Yan; Zhang, Juan; Lv, Zhao-Peng; Li, Yi-Xin

    2015-01-01

    Background: Macrophage apoptosis triggered by endoplasmic reticulum (ER) stress contributes much to atherosclerosis, especially plaque vulnerability. Activating transcription factor 4 (ATF4)-CCAAT/enhancer binding protein (C/EBP) homologous protein (CHOP)-Tribbles 3 (TRIB3) pathway is closely related to the ER stress. This study aimed to investigate the effect of atorvastatin on the ATF4-CHOP-TRIB3 pathway. Methods: Forty-seven patients were randomized into 80-mg and 20-mg atorvastatin group. Follow-up was performed at weeks 6 and 12, and complete blood chemistry, lipid assay and detection of 5 target genes (tumor protein 53, ATF4, C/EBP, CHOP and TRIB3) in monocytes/macrophages were conducted. Furthermore, the interaction between dosage and duration of therapy was evaluated. Results: After 12-week therapy, patients in both groups experienced significant reductions in ATF4 (P=0.038) and C/EBP (P=0.003) expressions. Tumor protein 53 (P=0.015) and TRIB3 (P=0.045) expressions increased markedly in 80-mg atorvastatin group. However, there was no significant difference in CHOP expression at three time-points and between atorvastatin groups. Moreover, there was no interaction between dosage and duration of therapy. Conclusions: Atorvastatin has an effect on ER stress through ATF4-CHOP pathway. Atorvastatin at a high dose is more likely to increase TRIB3 expression, but this warrants further investigation. PMID:26885117

  9. ATF6α/β-mediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish

    PubMed Central

    Ishikawa, Tokiro; Okada, Tetsuya; Ishikawa-Fujiwara, Tomoko; Todo, Takeshi; Kamei, Yasuhiro; Shigenobu, Shuji; Tanaka, Minoru; Saito, Taro L.; Yoshimura, Jun; Morishita, Shinichi; Toyoda, Atsushi; Sakaki, Yoshiyuki; Taniguchi, Yoshihito; Takeda, Shunichi; Mori, Kazutoshi

    2013-01-01

    ATF6α and ATF6β are membrane-bound transcription factors activated by regulated intramembrane proteolysis in response to endoplasmic reticulum (ER) stress to induce various ER quality control proteins. ATF6α- and ATF6β single-knockout mice develop normally, but ATF6α/β double knockout causes embryonic lethality, the reason for which is unknown. Here we show in medaka fish that ATF6α is primarily responsible for transcriptional induction of the major ER chaperone BiP and that ATF6α/β double knockout, but not ATF6α- or ATF6β single knockout, causes embryonic lethality, as in mice. Analyses of ER stress reporters reveal that ER stress occurs physiologically during medaka early embryonic development, particularly in the brain, otic vesicle, and notochord, resulting in ATF6α- and ATF6β-mediated induction of BiP, and that knockdown of the α1 chain of type VIII collagen reduces such ER stress. The absence of transcriptional induction of several ER chaperones in ATF6α/β double knockout causes more profound ER stress and impaired notochord development, which is partially rescued by overexpression of BiP. Thus ATF6α/β-mediated adjustment of chaperone levels to increased demands in the ER is essential for development of the notochord, which synthesizes and secretes large amounts of extracellular matrix proteins to serve as the body axis before formation of the vertebra. PMID:23447699

  10. Sound transmission loss of integrally damped, curved panels

    NASA Technical Reports Server (NTRS)

    Grosveld, Ferdinand W.; Reed, Samuel A.

    1989-01-01

    Results are reported from acoustic transmission-loss measurements on 13 curved Al and composite aircraft-type panels (height 0.81 m, arc length 1.32 m, and curvature radius 2.29 m) without and with integral damping of various types. The fabrication of the panels and the test procedures are described and illustrated with photographs, and the results are presented in graphs. It is found that the loss of a curved panel exceeds the mass-law-predicted loss for a flat panel of the same material and thickness at frequencies below the ring frequency. At higher frequencies, the curved-panel loss is lower than the mass-law loss, being proportional to 20 time the log of thickness. Integral damping is found to be effective both below and above the ring frequency, but different mechanisms are responsible in each case.

  11. Planetary Rings

    NASA Astrophysics Data System (ADS)

    Gordon, M. K.; Araki, S.; Black, G. J.; Bosh, A. S.; Brahic, A.; Brooks, S. M.; Charnoz, S.; Colwell, J. E.; Cuzzi, J. N.; Dones, L.; Durisen, R. H.; Esposito, L. W.; Ferrari, C.; Festou, M.; French, R. G.; Giuliatti-Winter, S. M.; Graps, A. L.; Hamilton, D. P.; Horanyi, M.; Karjalainen, R. M.; Krivov, A. V.; Krueger, H.; Larson, S. M.; Levison, H. F.; Lewis, M. C.; Lissauer, J. J.; Murray, C. D.; Namouni, F.; Nicholson, P. D.; Olkin, C. B.; Poulet, F.; Rappaport, N. J.; Salo, H. J.; Schmidt, J.; Showalter, M. R.; Spahn, F.; Spilker, L. J.; Srama, R.; Stewart, G. R.; Yanamandra-Fisher, P.

    2002-08-01

    The past two decades have witnessed dramatic changes in our view and understanding of planetary rings. We now know that each of the giant planets in the Solar System possesses a complex and unique ring system. Recent studies have identified complex gravitational interactions between the rings and their retinues of attendant satellites. Among the four known ring systems, we see elegant examples of Lindblad and corotation resonances (first invoked in the context of galactic disks), electromagnetic resonances, spiral density waves and bending waves, narrow ringlets which exhibit internal modes due to collective instabilities, sharp-edged gaps maintained via tidal torques from embedded moonlets, and tenuous dust belts created by meteoroid impact onto, or collisions between, parent bodies. Yet, as far as we have come, our understanding is far from complete. The fundamental questions confronting ring scientists at the beginning of the twenty-first century are those regarding the origin, age and evolution of the various ring systems, in the broadest context. Understanding the origin and age requires us to know the current ring properties, and to understand the dominant evolutionary processes and how they influence ring properties. Here we discuss a prioritized list of the key questions, the answers to which would provide the greatest improvement in our understanding of planetary rings. We then outline the initiatives, missions, and other supporting activities needed to address those questions, and recommend priorities for the coming decade in planetary ring science.

  12. Damping Vibration at an Impeller

    NASA Technical Reports Server (NTRS)

    Hager, J. A.; Rowan, B. F.

    1982-01-01

    Vibration of pump shaft is damped at impeller--where vibration-induced deflections are greatest--by shroud and seal. Damping reduces vibrational motion of shaft at bearings and load shaft places on them. Flow through clearance channel absorbs vibration energy.

  13. The Latest Results from DAMPE

    NASA Astrophysics Data System (ADS)

    Chang, Jin

    2016-07-01

    DArk Matter Particle Explorer (DAMPE) successfully launched on Dec.17, 2015 is the first Chinese astronomical satellite that can measure 2 GeV-10 TeV electrons and gamma-rays with unprecedented energy resolution. In this talk I will introduce the design, the beam-test, the on-orbit calibration and some preliminary results of DAMPE.

  14. Surge-damping vacuum valve

    DOEpatents

    Bullock, Jack C.; Kelly, Benjamin E.

    1980-01-01

    A valve having a mechanism for damping out flow surges in a vacuum system which utilizes a slotted spring-loaded disk positioned adjacent the valve's vacuum port. Under flow surge conditions, the differential pressure forces the disk into sealing engagement with the vacuum port, thereby restricting the flow path to the slots in the disk damping out the flow surge.

  15. Emittance and lifetime measurement with damping wigglers.

    PubMed

    Wang, G M; Shaftan, T; Cheng, W X; Guo, W; Ilinsky, P; Li, Y; Podobedov, B; Willeke, F

    2016-03-01

    National Synchrotron Light Source II (NSLS-II) is a new third-generation storage ring light source at Brookhaven National Laboratory. The storage ring design calls for small horizontal emittance (<1 nm-rad) and diffraction-limited vertical emittance at 12 keV (8 pm-rad). Achieving low value of the beam size will enable novel user experiments with nm-range spatial and meV-energy resolution. The high-brightness NSLS-II lattice has been realized by implementing 30-cell double bend achromatic cells producing the horizontal emittance of 2 nm rad and then halving it further by using several Damping Wigglers (DWs). This paper is focused on characterization of the DW effects in the storage ring performance, namely, on reduction of the beam emittance, and corresponding changes in the energy spread and beam lifetime. The relevant beam parameters have been measured by the X-ray pinhole camera, beam position monitors, beam filling pattern monitor, and current transformers. In this paper, we compare the measured results of the beam performance with analytic estimates for the complement of the 3 DWs installed at the NSLS-II. PMID:27036766

  16. Emittance and lifetime measurement with damping wigglers

    NASA Astrophysics Data System (ADS)

    Wang, G. M.; Shaftan, T.; Cheng, W. X.; Guo, W.; Ilinsky, P.; Li, Y.; Podobedov, B.; Willeke, F.

    2016-03-01

    National Synchrotron Light Source II (NSLS-II) is a new third-generation storage ring light source at Brookhaven National Laboratory. The storage ring design calls for small horizontal emittance (<1 nm-rad) and diffraction-limited vertical emittance at 12 keV (8 pm-rad). Achieving low value of the beam size will enable novel user experiments with nm-range spatial and meV-energy resolution. The high-brightness NSLS-II lattice has been realized by implementing 30-cell double bend achromatic cells producing the horizontal emittance of 2 nm rad and then halving it further by using several Damping Wigglers (DWs). This paper is focused on characterization of the DW effects in the storage ring performance, namely, on reduction of the beam emittance, and corresponding changes in the energy spread and beam lifetime. The relevant beam parameters have been measured by the X-ray pinhole camera, beam position monitors, beam filling pattern monitor, and current transformers. In this paper, we compare the measured results of the beam performance with analytic estimates for the complement of the 3 DWs installed at the NSLS-II.

  17. Damping seal verification setup

    NASA Technical Reports Server (NTRS)

    Cappel, K. L.

    1985-01-01

    The heart of the Space Shuttle Main Engine (SSME) is a set of turbopumps that propel cryogenic fluids at very high pressures and flow rates, at rotor speeds up to 37,000 rpm. Bushing seals that cause the flow in the fluid film to become turbulent, by means of a multiplicity of pockets, were shown theoretically not only to inhibit subsynchronous whirl, but to reduce leakage as well. However, experimental data that relate these two desirable characteristics to such parameters as pocket depth, Reynolds number (based on clearance and axial flow rate), and rotating speed are limited. To obtain the required data, NASA's Marshall Space Flight Center (MSFC) commissioned Wyle Laboratories to design, build and operate a test rig in which the damping efficacy and leakage reduction of typical candidate seals are to be evaluated.

  18. Endoplasmic reticulum stress signal impairs erythropoietin production: a role for ATF4.

    PubMed

    Chiang, Chih-Kang; Nangaku, Masaomi; Tanaka, Tetsuhiro; Iwawaki, Takao; Inagi, Reiko

    2013-02-15

    Hypoxia upregulates the hypoxia-inducible factor (HIF) pathway and the endoplasmic reticulum (ER) stress signal, unfolded protein response (UPR). The cross talk of both signals affects the pathogenic alteration by hypoxia. Here we showed that ER stress induced by tunicamycin or thapsigargin suppressed inducible (CoCl(2) or hypoxia) transcription of erythropoietin (EPO), a representative HIF target gene, in HepG2. This suppression was inversely correlated with UPR activation, as estimated by expression of the UPR regulator glucose-regulated protein 78, and restored by an ER stress inhibitor, salubrinal, in association with normalization of the UPR state. Importantly, the decreased EPO expression was also observed in HepG2 overexpressing UPR activating transcription factor (ATF)4. Overexpression of mutated ATF4 that lacks the transcriptional activity did not alter EPO transcriptional regulation. Transcriptional activity of the EPO 3'-enhancer, which is mainly regulated by HIF, was abolished by both ER stressors and ATF4 overexpression, while nuclear HIF accumulation or expression of other HIF target genes was not suppressed by ER stress. Chromatin immunoprecipitation analysis identified a novel ATF4 binding site (TGACCTCT) within the EPO 3'-enhancer region, suggesting a distinct role for ATF4 in UPR-dependent suppression of the enhancer. Induction of ER stress in rat liver and kidney by tunicamycin decreased the hepatic and renal mRNA and plasma level of EPO. Collectively, ER stress selectively impairs the transcriptional activity of EPO but not of other HIF target genes. This effect is mediated by suppression of EPO 3'-enhancer activity via ATF4 without any direct effect on HIF, indicating that UPR contributes to oxygen-sensing regulation of EPO. PMID:23242184

  19. 28 CFR 16.106 - Exemption of the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF)-Limited Access.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Exemption of Records Systems Under... Investigation Report System (JUSTICE/ATF-003). (2) These exemptions apply only to the extent that information in...) Internal Security Record System (JUSTICE/ATF-006). (2) These exemptions apply only to the extent...

  20. 28 CFR 16.106 - Exemption of the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF)-Limited Access.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Exemption of Records Systems Under... Investigation Report System (JUSTICE/ATF-003). (2) These exemptions apply only to the extent that information in...) Internal Security Record System (JUSTICE/ATF-006). (2) These exemptions apply only to the extent...

  1. 28 CFR 16.106 - Exemption of the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF)-Limited Access.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Exemption of Records Systems Under... Investigation Report System (JUSTICE/ATF-003). (2) These exemptions apply only to the extent that information in...) Internal Security Record System (JUSTICE/ATF-006). (2) These exemptions apply only to the extent...

  2. 28 CFR 16.106 - Exemption of the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF)-Limited Access.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Exemption of Records Systems Under... Investigation Report System (JUSTICE/ATF-003). (2) These exemptions apply only to the extent that information in...) Internal Security Record System (JUSTICE/ATF-006). (2) These exemptions apply only to the extent...

  3. JNK-mediated activation of ATF2 contributes to dopaminergic neurodegeneration in the MPTP mouse model of Parkinson's disease.

    PubMed

    Huang, Qiaoying; Du, Xiaoxiao; He, Xin; Yu, Qing; Hu, Kunhua; Breitwieser, Wolfgang; Shen, Qingyu; Ma, Shanshan; Li, Mingtao

    2016-03-01

    The c-Jun N-terminal kinase (JNK)/c-Jun pathway is a known critical regulator of dopaminergic neuronal death in Parkinson's disease (PD) and is considered a potential target for neuroprotective therapy. However, whether JNK is activated within dopaminergic neurons remains controversial, and whether JNK acts through downstream effectors other than c-Jun to promote dopaminergic neuronal death remains unclear. In this study, we confirm that JNK but not p38 is activated in dopaminergic neurons after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-intoxication. Furthermore, within the dopaminergic neurons of the substantia nigra in MPTP-treated mice, JNK2/3 phosphorylates threonine 69 (Thr69) of Activating transcription factor-2 (ATF2), a transcription factor of the ATF/CREB family, whereas the phosphorylation of Thr71 is constitutive and remains unchanged. The increased phosphorylation of ATF2 on Thr69 by JNK in the MPTP mouse model suggests a functional relationship between the transcriptional activation of ATF2 and dopaminergic neuron death. By using dopaminergic neuron-specific conditional ATF2 mutant mice, we found that either partial or complete deletion of the ATF2 DNA-binding domain in dopaminergic neurons markedly alleviates the MPTP-induced dopaminergic neurodegeneration, indicating that the activation of ATF2 plays a detrimental role in neuropathogenesis in PD. Taken together, our findings demonstrate that JNK-mediated ATF2 activation contributes to dopaminergic neuronal death in an MPTP model of PD. PMID:26515688

  4. Damping measurements using operational data

    SciTech Connect

    James, G.H.; Carne, T.G.; Veers, P.S.

    1996-08-01

    The authors have measured modal damping using strain-gauge data from an operating wind turbine. This new technique for measuring modal damping is easier and less expensive than previously used methods. Auto-correlation and cross-correlation functions of the strain-gauge data have been shown to consist of decaying sinusoids which correspond to the modal frequencies and damping ratios of the wind turbine. The authors have verified the method by extracting damping values from an analytically generated data set. Actual operating response data from the DOE/Sandia 34-m Test Bed has been used to calculate modal damping ratios as a function of rotor rotation rate. This capability will allow more accurate fatigue life prediction and control.

  5. Transverse instability at the recycler ring

    SciTech Connect

    Ng, K.Y.; /Fermilab

    2004-10-01

    Sporadic transverse instabilities have been observed at the Fermilab Recycler Ring leading to increase in transverse emittances and beam loss. The driving source of these instabilities has been attributed to the resistive-wall impedance with space-charge playing an important role in suppressing Landau damping. Growth rates of the instabilities are computed. Remaining problems are discussed.

  6. Damping Bearings In High-Speed Turbomachines

    NASA Technical Reports Server (NTRS)

    Von Pragenau, George L.

    1994-01-01

    Paper presents comparison of damping bearings with traditional ball, roller, and hydrostatic bearings in high-speed cryogenic turbopumps. Concept of damping bearings described in "Damping Seals and Bearings for a Turbomachine" (MFS-28345).

  7. ATF (Advanced Toroidal Facility) ECH (Electron Cyclotron Heating) waveguide component development and testing

    SciTech Connect

    Bigelow, T.S.; White, T.L.; Kimrey, H.D.

    1987-01-01

    The Advanced Toroidal Facility (ATF) Electron Cyclotron Heating (ECH) system presently under construction will consist of two 53.2-GHz, 200-kW continuous-wave (cw) gyrotrons with a mode-controlled waveguide system and polarized launcher optimized for maximum power in the ATF plasma. Several components, such as a waveguide mode-analyzing directional coupler, a TiO/sub 2/ mode absorber, miter bends, and a polarization-selectable beamed launcher, have been developed and tested. Laboratory results and initial high-power operation of the system are presented. 2 refs., 2 figs.

  8. A Comprehensive Study of Nanometer Resolution of the IPBPM at ATF2

    SciTech Connect

    Kim, Y.I.; Park, H.; Boogert, S.T.; Frisch, J.; McCormick, D.; Nelson, J.; Smith, T.; White, G.R.; Woodley, M.; Honda, Y.; Sugahara, R.; Terunuma, N.; Tauchi, T.; Urakawa, J.; /aff KEK, Tsukuba

    2011-12-13

    High-resolution beam position monitors (IPBPMs) have been developed in order to measure the electron beam position at the focus point of ATF2 to a few nanometers in the vertical plane. To date, the IPBPM system has operated in test mode with a highest demonstrated resolution of 8.7 nm in the ATF extraction line during 2008. After expected noise source calculations there still remains 7.9 nm of noise of unexplained origin. We summarize the experimental work on the IPBPM system since this measurement and outline the possible origins of these sources. We then present a study plan to be performed at the ATF2 facility designed to identify and to improve the resolution performance and comment on the expected ultimate resolution of this system. The Accelerator Test Facility 2 (ATF2) is a test beamline for ILC final focus system in the framework of the ATF international collaboration which was constructed to extend the extraction line at ATF, located at KEK, Japan. There are two goals of the ATF2: firstly to demonstrate focusing to 37 nm vertical beam size, secondly to achieve a few nanometer level beam orbit stability at the focus point in the vertical plane. High-resolution beam position monitors (IPBPMs) for the interaction point (IP) have been developed in order to measure the electron beam position at the focus point of the ATF2 to a few nanometers in the vertical plane. The previous measured position resolution of IPBPMs was 8.7 nm for a 0.68 x 10{sup 10} e/bunch beam with a dynamic range of 5 {mu}m. The intrinsic noise of the system was estimated to be 2.6 nm at 10{sup 10} e/bunch. It is scaled to 3.8 nm at 0.68 x 10{sup 10} e/bunch which means that 7.9 nm of unknown noise remains. The origin of the unknown noise must be studied in order to improve the resolution. This paper describes the ongoing work to improve the resolution of IPBPMs.

  9. Design of bunch compressing system with suppression of coherent synchrotron radiation for ATF upgrade

    SciTech Connect

    Jing, Yichao; Fedurin, Mikhail; Stratakis, Diktys

    2015-05-03

    One of the operation modes for Accelerator Test Facility (ATF) upgrade is to provide high peak current, high quality electron beam for users. Such operation requires a bunch compressing system with a very large compression ratio. The CSR originating from the strong compressors generally could greatly degrade the quality of the electron beam. In this paper, we present our design for the entire bunch compressing system that will limit the effect of CSR on the e-beam’s quality. We discuss and detail the performance from the start to end simulation of such a compressor for ATF.

  10. CHAC1/MGC4504 is a novel proapoptotic component of the unfolded protein response, downstream of the ATF4-ATF3-CHOP cascade.

    PubMed

    Mungrue, Imran N; Pagnon, Joanne; Kohannim, Omid; Gargalovic, Peter S; Lusis, Aldons J

    2009-01-01

    To understand pathways mediating the inflammatory responses of human aortic endothelial cells to oxidized phospholipids, we previously used a combination of genetics and genomics to model a coexpression network encompassing >1000 genes. CHAC1 (cation transport regulator-like protein 1), a novel gene regulated by ox-PAPC (oxidized 1-palmitoyl-2-arachidonyl-sn-3-glycero-phosphorylcholine), was identified in a co-regulated group of genes enriched for components of the ATF4 (activating transcription factor 4) arm of the unfolded protein response pathway. Herein, we characterize the role of CHAC1 and validate the network model. We first define the activation of CHAC1 mRNA by chemical unfolded protein response-inducers, but not other cell stressors. We then define activation of CHAC1 by the ATF4-ATF3-CHOP (C/EBP homologous protein), and not parallel XBP1 (X box-binding protein 1) or ATF6 pathways, using siRNA and/or overexpression plasmids. To examine the subset of genes downstream of CHAC1, we used expression microarray analysis to identify a list of 227 differentially regulated genes. We validated the activation of TNFRSF6B (tumor necrosis factor receptor superfamily, member 6b), a FASL decoy receptor, in cells treated with CHAC1 small interfering RNA. Finally, we showed that CHAC1 overexpression enhanced apoptosis, while CHAC1 small interfering RNA suppressed apoptosis, as determined by TUNEL, PARP (poly(ADP-ribose) polymerase) cleavage, and AIF (apoptosis-inducing factor) nuclear translocation. PMID:19109178

  11. Mechanism of action for NNZ-2566 anti-inflammatory effects following PBBI involves upregulation of immunomodulator ATF3.

    PubMed

    Cartagena, Casandra M; Phillips, Katie L; Williams, Garry L; Konopko, Melissa; Tortella, Frank C; Dave, Jitendra R; Schmid, Kara E

    2013-09-01

    The tripeptide glycine-proline-glutamate analogue NNZ-2566 (Neuren Pharmaceuticals) demonstrates neuroprotective efficacy in models of traumatic brain injury. In penetrating ballistic-like brain injury (PBBI), it significantly decreases injury-induced upregulation of inflammatory cytokines including TNF-α, IFN-γ, and IL-6. However, the mechanism by which NNZ-2566 acts has yet to be determined. The activating transcription factor-3 (ATF3) is known to repress expression of these inflammatory cytokines and was increased at the mRNA and protein level 24-h post-PBBI. This study investigated whether 12 h of NNZ-2566 treatment following PBBI alters atf3 expression. PBBI alone significantly increased atf3 mRNA levels by 13-fold at 12 h and these levels were increased by an additional fourfold with NNZ-2566 treatment. To confirm that changes in mRNA translated to changes in protein expression, ATF3 expression levels were determined in vivo in microglia/macrophages, T cells, natural killer cells (NKCs), astrocytes, and neurons. PBBI alone significantly increased ATF3 in microglia/macrophages (820%), NKCs (58%), and astrocytes (51%), but decreased levels in T cells (48%). NNZ-2566 treatment further increased ATF3 protein expression in microglia/macrophages (102%), NKCs (308%), and astrocytes (13%), while reversing ATF3 decreases in T cells. Finally, PBBI increased ATF3 levels by 55% in neurons and NNZ-2566 treatment further increased these levels an additional 33%. Since increased ATF3 may be an innate protective mechanism to limit inflammation following injury, these results demonstrating that the anti-inflammatory and neuroprotective drug NNZ-2566 increase both mRNA and protein levels of ATF3 in multiple cell types provide a cellular mechanism for NNZ-2566 modulation of neuroinflammation following PBBI. PMID:23765588

  12. Expression of a phosphorylated form of ATF4 in lung and non-small cell lung cancer tissues.

    PubMed

    Fan, Chui-Feng; Miao, Yuan; Lin, Xu-Yong; Zhang, Di; Wang, En-Hua

    2014-01-01

    ATF4 is a member of the cAMP-responsive element-binding protein family of basic zipper-containing proteins, a family of transcription factors phosphorylated at serines residues by protein kinase A. The family has been proved to be able to stimulate the transcription of the genes containing CRE elements. Elevated ATF4 expression was detected in some tumors including breast carcinoma compared to their corresponding nontumor tissues. p-ATF4 (ser 245), a phosphorylated form of ATF4 protein at serine 245 site, was believed to be an active type of this protein. However, its expression and clinical significance in malignant tumors including non-small cell lung cancer were not reported up to date. In the current study, we investigate the expression of p-ATF4 (ser 245) in non-small cell lung cancer using tissue microarray and immunohistochemistry. p-ATF4 (ser 245) immunostaining was detected in nucleus and cytoplasm in cancer cells and normal lung epithelial cells. Compared to bronchial epithelium and submucosal glands (total positive rate, 14.6% (12/82)), there was increased expression of p-ATF4 (ser 245) in non-small cell lung cancer cells (total positive rate, 42.7% (35/82)) (p < 0.05). In addition, increased expression of p-ATF4 (ser 245) was associated with lymph node metastasis and advanced TNM stages (III and IV) in non-small cell lung cancer (p < 0.05). Immunofluorescent staining confirmed nuclear and cytoplasmic expression of p-ATF4 (ser 245) in lung and cancer tissues, and also in non-small cell lung cancer cell lines including NCI-H157 and LTE cells. These results indicate that increased expression of p-ATF4 (ser 245) may contribute to cancer development of non-small cell lung cancer and may be a potential cancer marker. PMID:23975372

  13. Fast and reliable kicker magnets for the SLC damping rings

    SciTech Connect

    Mattison, T.S.; Cassel, R.L.; Donaldson, A.R.; Gross, G.

    1995-06-01

    The design, construction, and operation of a kicker magnet with superior electromagnetic performance and greatly improved radiation tolerance is described. A short flux return of high mu ferrite improves the field strength and linearity with current, and novel metallic field-confining structures minimize the inductance. An 8-cell structure with capacitance integrated into each cell makes the magnet a nearly perfect transmission line. The capacitor dielectric is 1 cm thick alumina-loaded epoxy, processed to eliminate air voids, and cast in a multiple step procedure developed to circumvent epoxy shrinkage. The magnet operates with pulses of up to 40 kV and 3.2 kA at 120 Hz, with magnet transit times of less than 35 nsec and field rise and fall times of less than 60 nsec.

  14. Tests and analysis for SLC damping ring data

    SciTech Connect

    Spencer, J.E.

    1993-04-01

    Beyond typical, open-loop or feedback use, fast, turn-by-turn bunch monitors provide recursive data that determines system dimensionality d and K-entropy whose time fluctuations provide useful diagnostic tools. For injection and extraction, one can study variations in the dimensionality of the attractor with matching, jitter, current and tunes. Comparison to dynamical models then helps to optimize control. Experimental and theoretical examples and their signatures are discussed e.g. the evolution of the distribution function after injection is studied as a solution of Liouville`s equation.

  15. Elastic ring deformation and pedestal contact status analysis of elastic ring squeeze film damper

    NASA Astrophysics Data System (ADS)

    Zhang, Wei; Ding, Qian

    2015-06-01

    This paper investigates the dynamic parametric characteristic of the elastic ring squeeze film damper (ERSFD). Firstly, the coupled oil film Reynolds equations and dynamic equations of an ERSFD supported rotor system are established. The finite differential method and numerical simulation are used to analyze the oil film pressure distribution, bearing capacity of ERSFD, oil film stiffness and damping characteristics during a vibration period. Then, based on the oil film pressure results, the deformation of elastic ring is revealed by the finite element method. Finally, pedestal contact status is analyzed according to the change of oil film thickness during a vibration period. The results reveal that the oil film pressure is sectionally continuous, the deformation of elastic ring is complex under the compression of inner and outer oil film, and different pedestal contacts occur in a vibration period. The level of nonlinearity of the bearing capacity, oil film stiffness and damping can be effectively lightened by application of the elastic ring.

  16. Planetary Rings

    NASA Technical Reports Server (NTRS)

    Cuzzi, Jeffrey N.

    1994-01-01

    Just over two decades ago, Jim Pollack made a critical contribution to our understanding of planetary ring particle properties, and resolved a major apparent paradox between radar reflection and radio emission observations. At the time, particle properties were about all there were to study about planetary rings, and the fundamental questions were, why is Saturn the only planet with rings, how big are the particles, and what are they made of? Since then, we have received an avalanche of observations of planetary ring systems, both from spacecraft and from Earth. Meanwhile, we have seen steady progress in our understanding of the myriad ways in which gravity, fluid and statistical mechanics, and electromagnetism can combine to shape the distribution of the submicron-to-several-meter size particles which comprise ring systems into the complex webs of structure that we now know them to display. Insights gained from studies of these giant dynamical analogs have carried over into improved understanding of the formation of the planets themselves from particle disks, a subject very close to Jim's heart. The now-complete reconnaissance of the gas giant planets by spacecraft has revealed that ring systems are invariably found in association with families of regular satellites, and there is ark emerging perspective that they are not only physically but causally linked. There is also mounting evidence that many features or aspects of all planetary ring systems, if not the ring systems themselves, are considerably younger than the solar system

  17. Self-Damping Sprung Wheel

    NASA Technical Reports Server (NTRS)

    Weddendorf, Bruce

    1993-01-01

    Self-damping sprung wheel provides shock-absorbing suspension for wheelchair, reducing user's discomfort when traversing rough terrain or obstacles. Pair of self-damping sprung wheels installed in place of conventional large rear wheels of standard wheelchair, which user operates in conventional manner. Rim deflects in vicinity of contact with ground or floor. Includes inner and outer hoops bending when obstacle encountered. Shear deformation of elastomeric hoop between them absorbs energy. Thus, three hoops act together as damping spring. Alternative version of wheel designed for bicycle.

  18. Validation of High-Resolution CFD Method for Slosh Damping Extraction of Baffled Tanks

    NASA Technical Reports Server (NTRS)

    Yang, H. Q.; West, Jeff

    2016-01-01

    Determination of slosh damping is a very challenging task as there is no analytical solution. The damping physics involve the vorticity dissipation which requires the full solution of the nonlinear Navier-Stokes equations. As a result, previous investigations and knowledge were mainly carried out by extensive experimental studies. A Volume-Of-Fluid (VOF) based CFD program developed at NASA MSFC was applied to extract slosh damping in a baffled tank from the first principle. First, experimental data using water with subscale smooth wall tank were used as the baseline validation. CFD simulation was demonstrated to be capable of accurately predicting natural frequency and very low damping value from the smooth wall tank at different fill levels. The damping due to a ring baffle at different liquid fill levels from barrel section and into the upper dome was then investigated to understand the slosh damping physics due to the presence of a ring baffle. Based on this study, the Root-Mean-Square error of our CFD simulation in estimating slosh damping was less than 4.8%, and the maximum error was less than 8.5%. Scalability of subscale baffled tank test using water was investigated using the validated CFD tool, and it was found that unlike the smooth wall case, slosh damping with baffle is almost independent of the working fluid and it is reasonable to apply water test data to the full scale LOX tank when the damping from baffle is dominant. On the other hand, for the smooth wall, the damping value must be scaled according to the Reynolds number. Comparison of experimental data, CFD, with the classical and modified Miles equations for upper dome was made, and the limitations of these semi-empirical equations were identified.

  19. Tumor suppressor BTG1 promotes PRMT1-mediated ATF4 function in response to cellular stress

    PubMed Central

    Tijchon, Esther; van Ingen Schenau, Dorette; van Emst, Liesbeth; Levers, Marloes; Palit, Sander A.L.; Rodenbach, Caroline; Poelmans, Geert; Hoogerbrugge, Peter M.; Shan, Jixiu; Kilberg, Michael S.; Scheijen, Blanca; van Leeuwen, Frank N.

    2016-01-01

    Cancer cells are frequently exposed to physiological stress conditions such as hypoxia and nutrient limitation. Escape from stress-induced apoptosis is one of the mechanisms used by malignant cells to survive unfavorable conditions. B-cell Translocation Gene 1 (BTG1) is a tumor suppressor that is frequently deleted in acute lymphoblastic leukemia and recurrently mutated in diffuse large B cell lymphoma. Moreover, low BTG1 expression levels have been linked to poor outcome in several solid tumors. How loss of BTG1 function contributes to tumor progression is not well understood. Here, using Btg1 knockout mice, we demonstrate that loss of Btg1 provides a survival advantage to primary mouse embryonic fibroblasts (MEFs) under stress conditions. This pro-survival effect involves regulation of Activating Transcription Factor 4 (ATF4), a key mediator of cellular stress responses. We show that BTG1 interacts with ATF4 and positively modulates its activity by recruiting the protein arginine methyl transferase PRMT1 to methylate ATF4 on arginine residue 239. We further extend these findings to B-cell progenitors, by showing that loss of Btg1 expression enhances stress adaptation of mouse bone marrow-derived B cell progenitors. In conclusion, we have identified the BTG1/PRMT1 complex as a new modifier of ATF4 mediated stress responses. PMID:26657730

  20. FIAT represses ATF4-mediated transcription to regulate bone mass in transgenic mice.

    PubMed

    Yu, Vionnie W C; Ambartsoumian, Gourgen; Verlinden, Lieve; Moir, Janet M; Prud'homme, Josée; Gauthier, Claude; Roughley, Peter J; St-Arnaud, René

    2005-05-23

    We report the characterization of factor inhibiting activating transcription factor 4 (ATF4)-mediated transcription (FIAT), a leucine zipper nuclear protein. FIAT interacted with ATF4 to inhibit binding of ATF4 to DNA and block ATF4-mediated transcription of the osteocalcin gene in vitro. Transgenic mice overexpressing FIAT in osteoblasts also had reduced osteocalcin gene expression and decreased bone mineral density, bone volume, mineralized volume, trabecular thickness, trabecular number, and decreased rigidity of long bones. Mineral homeostasis, osteoclast number and activity, and osteoblast proliferation and apoptosis were unchanged in transgenics. Expression of osteoblastic differentiation markers was largely unaffected and type I collagen synthesis was unchanged. Mineral apposition rate was reduced in transgenic mice, suggesting that the lowered bone mass was due to a decline in osteoblast activity. This cell-autonomous decrease in osteoblast activity was confirmed by measuring reduced alkaline phosphatase activity and mineralization in primary osteoblast cultures. These results show that FIAT regulates bone mass accrual and establish FIAT as a novel transcriptional regulator of osteoblastic function. PMID:15911876

  1. The Transcription Factor EB (TFEB) Regulates Osteoblast Differentiation Through ATF4/CHOP-Dependent Pathway.

    PubMed

    Yoneshima, Erika; Okamoto, Kuniaki; Sakai, Eiko; Nishishita, Kazuhisa; Yoshida, Noriaki; Tsukuba, Takayuki

    2016-06-01

    Osteoblasts are bone-forming cells that produce large amounts of collagen type I and various bone matrix proteins. Although osteoblast differentiation is highly regulated by various factors, it remains unknown whether lysosomes are directly involved in osteoblast differentiation. Here, we demonstrate the transcription factor EB (TFEB), a master regulator of lysosomal biogenesis, modulates osteoblast differentiation. The expression levels of TFEB as well as those of endosomal/lysosomal proteins were up-regulated during osteoblast differentiation using mouse osteoblastic MC3T3-E1 cells. By gene knockdown (KD) experiments with small interfering RNA (siRNA), TFEB depletion caused markedly reduced osteoblast differentiation as compared with the control cells. Conversely, overexpression (OE) of TFEB resulted in strikingly enhanced osteoblastogenesis compared to the control cells. By analysis of down-stream effector molecules, TFEB KD was found to cause marked up-regulation of activating transcription factor 4 (ATF4) and CCAAT/enhancer-binding protein homologous protein (CHOP), both of which are essential factors for osteoblastogenesis. In contrast, TFEB OE promoted osteoblast differentiation through reduced expression of ATF4 and CHOP without differentiation agents. Given the importance of ATF4 and CHOP in osteoblastogenesis, it is clear that the TFEB-regulated signaling pathway for osteoblast differentiation is involved in ATF4/CHOP-dependent signaling pathway. PMID:26519689

  2. Measurements of ECH absorption on ATF using a polarization-controlled beam launcher

    SciTech Connect

    Bigelow, T.S.; Schaich, C.R.; White, T.L.

    1989-01-01

    Electron cyclotron heating (ECH) is used on the Advanced Toroidal Facility (ATF) experiment at ORNL for plasma formation and heating. A 53.2 GHz gyrotron generates 200 kW cw which is transported to ATF in 6.35 cm evacuated waveguide. Power is launched into ATF using a recently completed polarization controlled beam launcher which can launch a linear polarized beam with a /minus/20 dB diameter of 12 cm at the plasma center. The launcher consists of a Vlasov mode converting antenna, a Teflon-copper laminate polarization rotating grating, and a spherical focusing mirror. The plane of polarization can be remotely adjusted by rotating the grating with a motorized vacuum feedthrough. First pass plasma absorption is monitored in two planes of polarization using a dual-polarized detector looking through a dome shaped scattering cut-off screen. During plasma operation, the detected signals indicate that absorption under ideal conditions is nearly complete. With low density or a shifted resonance zone, absorption is small and there are cases where there is mode coupling to the perpendicular polarization. This is presumably due to shear in the ATF magnetic field. 2 refs., 3 figs.

  3. 77 FR 70467 - Agency Information Collection Activities: Proposed Collection; Comments Requested; ATF...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-26

    ... of Alcohol, Tobacco, Firearms and Explosives Agency Information Collection Activities: Proposed... Department of Justice (DOJ), Bureau of Alcohol, Tobacco, Firearms and Explosives (ATF) will be submitting the... F 1370.4. Bureau of Alcohol, Tobacco, Firearms and Explosives. (4) Affected public who will be...

  4. ATF3 Expression in the corpus luteum: possible role in luteal regression

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The present study investigated the induction and possible role of activating transcription factor 3 (ATF3) in the corpus luteum. Postpubertal cattle were treated at midcycle with prostaglandin F2alpha(PGF) for 0–4 hours. Luteal tissue was processed for immunohistochemistry, in situ hybridization, an...

  5. Numerical study of self modulation instability of 1 nC electron bunch at ATF

    SciTech Connect

    Fang Yun; Mori, Warren; Muggli, Patric

    2012-12-21

    The development of self-modulation instability (SMI) is investigated numerically for the 1 nC electron bunch available at Accelerator Test Facility (ATF) of Brookhaven National Laboratory (BNL). Possible experiment based on the simulation results is proposed. All the simulations are performed with the 2D-cylindrically symmetric particle-in-cell code.

  6. KDM4C and ATF4 Cooperate in Transcriptional Control of Amino Acid Metabolism.

    PubMed

    Zhao, Erhu; Ding, Jane; Xia, Yingfeng; Liu, Mengling; Ye, Bingwei; Choi, Jeong-Hyeon; Yan, Chunhong; Dong, Zheng; Huang, Shuang; Zha, Yunhong; Yang, Liqun; Cui, Hongjuan; Ding, Han-Fei

    2016-01-26

    The histone lysine demethylase KDM4C is often overexpressed in cancers primarily through gene amplification. The molecular mechanisms of KDM4C action in tumorigenesis are not well defined. Here, we report that KDM4C transcriptionally activates amino acid biosynthesis and transport, leading to a significant increase in intracellular amino acid levels. Examination of the serine-glycine synthesis pathway reveals that KDM4C epigenetically activates the pathway genes under steady-state and serine deprivation conditions by removing the repressive histone modification H3 lysine 9 (H3K9) trimethylation. This action of KDM4C requires ATF4, a transcriptional master regulator of amino acid metabolism and stress responses. KDM4C activates ATF4 transcription and interacts with ATF4 to target serine pathway genes for transcriptional activation. We further present evidence for KDM4C in transcriptional coordination of amino acid metabolism and cell proliferation. These findings suggest a molecular mechanism linking KDM4C-mediated H3K9 demethylation and ATF4-mediated transactivation in reprogramming amino acid metabolism for cancer cell proliferation. PMID:26774480

  7. KDM4C and ATF4 Cooperate in Transcriptional Control of Amino Acid Metabolism

    PubMed Central

    Xia, Yingfeng; Liu, Mengling; Ye, Bingwei; Choi, Jeong-Hyeon; Yan, Chunhong; Dong, Zheng; Huang, Shuang; Zha, Yunhong; Yang, Liqun; Cui, Hongjuan; Ding, Han-Fei

    2015-01-01

    SUMMARY The histone lysine demethylase KDM4C is often overexpressed in cancers primarily through gene amplification. The molecular mechanisms of KDM4C action in tumorigenesis are not well defined. Here we report that KDM4C transcriptionally activates amino acid biosynthesis and transport, leading to a significant increase in intracellular amino acid levels. Examination of the serine-glycine synthesis pathway reveals that KDM4C epigenetically activates the pathway genes under steady-state and serine deprivation conditions by removing the repressive histone modification H3 lysine 9 (H3K9) trimethylation. This action of KDM4C requires ATF4, a transcriptional master regulator of amino acid metabolism and stress responses. KDM4C activates ATF4 transcription and interacts with ATF4 to target serine pathway genes for transcriptional activation. We further present evidence for KDM4C in transcriptional coordination of amino acid metabolism and cell proliferation. These findings suggest a molecular mechanism linking KDM4C-mediated H3K9 demethylation and ATF4-mediated transactivation in reprogramming amino acid metabolism for cancer cell proliferation. PMID:26774480

  8. 78 FR 77494 - Agency Information Collection Activities; Proposed Collection; Comments Requested: ATF Adjunct...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF JUSTICE Bureau of Alcohol, Tobacco, Firearms and Explosives Agency Information Collection Activities; Proposed Collection; Comments Requested: ATF Adjunct Instructor Data Form ACTION: 60-Day Notice. The Department of Justice (DOJ), Bureau of Alcohol,...

  9. Gilbert damping in noncollinear ferromagnets.

    PubMed

    Yuan, Zhe; Hals, Kjetil M D; Liu, Yi; Starikov, Anton A; Brataas, Arne; Kelly, Paul J

    2014-12-31

    The precession and damping of a collinear magnetization displaced from its equilibrium are well described by the Landau-Lifshitz-Gilbert equation. The theoretical and experimental complexity of noncollinear magnetizations is such that it is not known how the damping is modified by the noncollinearity. We use first-principles scattering theory to investigate transverse domain walls (DWs) of the important ferromagnetic alloy Ni80Fe20 and show that the damping depends not only on the magnetization texture but also on the specific dynamic modes of Bloch and Néel DWs in ways that were not theoretically predicted. Even in the highly disordered Ni80Fe20 alloy, the damping is found to be remarkably nonlocal. PMID:25615368

  10. Damping measurements using operational data

    SciTech Connect

    James, G.H.; Carne, T.G.; Veers, P.S.

    1991-01-01

    We have measured modal damping using strain-gauge data from an operating wind turbine. Previously, such measurements were difficult and expensive. Auto-correlation and cross-correlation functions of the strain-gauge data have been shown to consist of decaying sinusoids which correspond to the modal frequencies and damping ratios of the wind turbine. We have verified the method by extracting damping values from an analytically generated data set. Actual operating response data from the DOE/Sandia 34-meter Test Bed has been used to calculate modal damping ratios as a function of rotor rotation rate. This capability will allow more accurate fatigue life prediction and control. 16 refs., 3 figs., 2 tabs.

  11. Vibration damping method and apparatus

    DOEpatents

    Redmond, James M.; Barney, Patrick S.; Parker, Gordon G.; Smith, David A.

    1999-01-01

    The present invention provides vibration damping method and apparatus that can damp vibration in more than one direction without requiring disassembly, that can accommodate varying tool dimensions without requiring re-tuning, and that does not interfere with tool tip operations and cooling. The present invention provides active dampening by generating bending moments internal to a structure such as a boring bar to dampen vibration thereof.

  12. Vibration damping method and apparatus

    DOEpatents

    Redmond, J.M.; Barney, P.S.; Parker, G.G.; Smith, D.A.

    1999-06-22

    The present invention provides vibration damping method and apparatus that can damp vibration in more than one direction without requiring disassembly, that can accommodate varying tool dimensions without requiring re-tuning, and that does not interfere with tool tip operations and cooling. The present invention provides active dampening by generating bending moments internal to a structure such as a boring bar to dampen vibration thereof. 38 figs.

  13. C/EBPγ Is a Critical Regulator of Cellular Stress Response Networks through Heterodimerization with ATF4.

    PubMed

    Huggins, Christopher J; Mayekar, Manasi K; Martin, Nancy; Saylor, Karen L; Gonit, Mesfin; Jailwala, Parthav; Kasoji, Manjula; Haines, Diana C; Quiñones, Octavio A; Johnson, Peter F

    2016-03-01

    The integrated stress response (ISR) controls cellular adaptations to nutrient deprivation, redox imbalances, and endoplasmic reticulum (ER) stress. ISR genes are upregulated in stressed cells, primarily by the bZIP transcription factor ATF4 through its recruitment to cis-regulatory C/EBP:ATF response elements (CAREs) together with a dimeric partner of uncertain identity. Here, we show that C/EBPγ:ATF4 heterodimers, but not C/EBPβ:ATF4 dimers, are the predominant CARE-binding species in stressed cells. C/EBPγ and ATF4 associate with genomic CAREs in a mutually dependent manner and coregulate many ISR genes. In contrast, the C/EBP family members C/EBPβ and C/EBP homologous protein (CHOP) were largely dispensable for induction of stress genes. Cebpg(-/-) mouse embryonic fibroblasts (MEFs) proliferate poorly and exhibit oxidative stress due to reduced glutathione levels and impaired expression of several glutathione biosynthesis pathway genes. Cebpg(-/-) mice (C57BL/6 background) display reduced body size and microphthalmia, similar to ATF4-null animals. In addition, C/EBPγ-deficient newborns die from atelectasis and respiratory failure, which can be mitigated by in utero exposure to the antioxidant, N-acetyl-cysteine. Cebpg(-/-) mice on a mixed strain background showed improved viability but, upon aging, developed significantly fewer malignant solid tumors than WT animals. Our findings identify C/EBPγ as a novel antioxidant regulator and an obligatory ATF4 partner that controls redox homeostasis in normal and cancerous cells. PMID:26667036

  14. The basic helix loop helix transcription factor Twist1 is a novel regulator of ATF4 in osteoblasts.

    PubMed

    Danciu, Theodora E; Li, Yan; Koh, Amy; Xiao, Guozhi; McCauley, Laurie K; Franceschi, Renny T

    2012-01-01

    Parathyroid hormone (PTH) is an essential regulator of endochondral bone formation and an important anabolic agent for the reversal of bone loss. PTH mediates its functions in part by regulating binding of the bone-related activating transcription factor 4 (ATF4) to the osteoblast-specific gene, osteocalcin. The basic helix-loop-helix (bHLH) factors Twist1 and Twist2 also regulate osteocalcin transcription in part through the interaction of the C-terminal "box" domain in these factors and Runx2. In this study, we discovered a novel function of PTH: its ability to dramatically decrease Twist1 transcription. Since ATF4 is a major regulator of the PTH response in osteoblasts, we assessed the mutual regulation between these factors and determined that Twist proteins and ATF4 physically interact in a manner that affects ATF4 DNA binding function. We mapped the interaction domain of Twist proteins to the C-terminal "box" domain and of ATF4, to the N-terminus. Furthermore, we demonstrate that Twist1 overexpression in osteoblasts attenuates ATF4 binding to the osteocalcin promoter in response to PTH. This study thus identifies Twist proteins as novel inhibitory binding partners of ATF4 and explores the functional significance of this interaction. PMID:21866569

  15. The basic helix loop helix transcription factor Twist1 is a novel regulator of ATF4 in osteoblasts

    PubMed Central

    Danciu, Theodora E.; Li, Yan; Koh, Amy; Xiao, Guozhi; McCauley, Laurie K.; Franceschi, Renny T.

    2011-01-01

    Parathyroid hormone (PTH) is an essential regulator of endochondral bone formation and an important anabolic agent for the reversal of bone loss. PTH mediates its functions in part by regulating binding of the bone-related activating transcription factor 4 (ATF4) to the osteoblast-specific gene, osteocalcin. The basic helix-loop-helix (bHLH) factors Twist1 and Twist2 also regulate osteocalcin transcription in part through the interaction of the C-terminal “box” domain in these factors and Runx2. In this study, we discovered a novel function of PTH: its ability to dramatically decrease Twist1 transcription. Since ATF4 is a major regulator of the PTH response in osteoblasts, we assessed the mutual regulation between these factors and determined that Twist proteins and ATF4 physically interact in a manner that affects ATF4 DNA binding function. We mapped the interaction domain of Twist proteins to the C-terminal “box” domain and of ATF4, to the N-terminus. Furthermore, we demonstrate that Twist1 overexpression in osteoblasts attenuates ATF4 binding to the osteocalcin promoter in response to PTH. This study thus identifies Twist proteins as novel inhibitory binding partners of ATF4 and explores the functional significance of this interaction. PMID:21866569

  16. Transcriptional cross talk between orphan nuclear receptor ERRγ and transmembrane transcription factor ATF6α coordinates endoplasmic reticulum stress response

    PubMed Central

    Misra, Jagannath; Kim, Don-Kyu; Choi, Woogyun; Koo, Seung-Hoi; Lee, Chul-Ho; Back, Sung-Hoon; Kaufman, Randal J.; Choi, Hueng-Sik

    2013-01-01

    Orphan nuclear receptor ERRγ is a member of nuclear receptor superfamily that regulates several important cellular processes including hepatic glucose and alcohol metabolism. However, mechanistic understanding of transcriptional regulation of the ERRγ gene remains to be elucidated. Here, we report that activating transcription factor 6α (ATF6α), an endoplasmic reticulum (ER)-membrane–bound basic leucine zipper (bZip) transcription factor, directly regulates ERRγ gene expression in response to ER stress. ATF6α binds to ATF6α responsive element in the ERRγ promoter. The transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α) is required for this transactivation. Chromatin immunoprecipitation (ChIP) assay confirmed the binding of both ATF6α and PGC1α on the ERRγ promoter. ChIP assay demonstrated histone H3 and H4 acetylation occurs at the ATF6α and PGC1α binding site. Of interest, ERRγ along with PGC1α induce ATF6α gene transcription upon ER stress. ERRγ binds to an ERRγ responsive element in the ATF6α promoter. ChIP assay confirmed that both ERRγ and PGC1α bind to a site in the ATF6α promoter that exhibits histone H3 and H4 acetylation. Overall, for the first time our data show a novel pathway of cross talk between nuclear receptors and ER-membrane–bound transcription factors and suggest a positive feed-forward loop regulates ERRγ and ATF6α gene transcription. PMID:23716639

  17. Transcriptional cross talk between orphan nuclear receptor ERRγ and transmembrane transcription factor ATF6α coordinates endoplasmic reticulum stress response.

    PubMed

    Misra, Jagannath; Kim, Don-Kyu; Choi, Woogyun; Koo, Seung-Hoi; Lee, Chul-Ho; Back, Sung-Hoon; Kaufman, Randal J; Choi, Hueng-Sik

    2013-08-01

    Orphan nuclear receptor ERRγ is a member of nuclear receptor superfamily that regulates several important cellular processes including hepatic glucose and alcohol metabolism. However, mechanistic understanding of transcriptional regulation of the ERRγ gene remains to be elucidated. Here, we report that activating transcription factor 6α (ATF6α), an endoplasmic reticulum (ER)-membrane-bound basic leucine zipper (bZip) transcription factor, directly regulates ERRγ gene expression in response to ER stress. ATF6α binds to ATF6α responsive element in the ERRγ promoter. The transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α) is required for this transactivation. Chromatin immunoprecipitation (ChIP) assay confirmed the binding of both ATF6α and PGC1α on the ERRγ promoter. ChIP assay demonstrated histone H3 and H4 acetylation occurs at the ATF6α and PGC1α binding site. Of interest, ERRγ along with PGC1α induce ATF6α gene transcription upon ER stress. ERRγ binds to an ERRγ responsive element in the ATF6α promoter. ChIP assay confirmed that both ERRγ and PGC1α bind to a site in the ATF6α promoter that exhibits histone H3 and H4 acetylation. Overall, for the first time our data show a novel pathway of cross talk between nuclear receptors and ER-membrane-bound transcription factors and suggest a positive feed-forward loop regulates ERRγ and ATF6α gene transcription. PMID:23716639

  18. The effects of the space environment on damping materials and damping designs on flexible structures

    NASA Technical Reports Server (NTRS)

    Kluesener, M. F.

    1984-01-01

    The effects of space environments on damping materials and damping designs on flexible structures were investigated. The following items were examined: damping of flexible spacecraft appendages; composite loss factor (n sub s) vs. time in high vacuum for damped test beams and damping of flexible structures. The STEP experiments show inherent damping of flexible structures in space effective possible damping design configurations for space structures, effects of passively damped components on the system loss factor of flexible structures and the effect of space environment on properties of damping materials.

  19. Activating transcription factor-3 (ATF3) functions as a tumor suppressor in colon cancer and is up-regulated upon heat-shock protein 90 (Hsp90) inhibition

    PubMed Central

    2010-01-01

    Background Activating transcription factor-3 (ATF3) is involved in the complex process of cellular stress response. However, its exact role in cancer is discussed controversially because both tumor suppressive and oncogenic effects have been described. Here we followed-up on our previous observation that inhibition of Hsp90 may increase ATF3 expression and sought to determine the role of ATF3 in colon cancer. Methods Regulation of ATF3 was determined in cancer cells using signaling inhibitors and a heat-shock protein-90 (Hsp90) antagonist. Human HCT116 cancer cells were stably transfected with an ATF3-shRNA or a luciferase-shRNA expression plasmid and alterations in cell motility were assessed in migration assays. The impact of ATF3 down-regulation on cancer growth and metastasis were investigated in a subcutaneous tumor model, a model of hepatic tumor growth and in a model of peritoneal carcinomatosis. Human colon cancer tissues were analyzed for ATF3 expression. Results The results show that therapeutic Hsp90 inhibition substantially up-regulates the expression of ATF3 in various cancer cells, including colon, gastric and pancreatic cancer. This effect was evident both in vitro and in vivo. RNAi mediated knock-down of ATF3 in HCT116 colon cancer cells significantly increased cancer cell migration in vitro. Moreover, in xenogenic mouse models, ATF3 knock-down promoted subcutaneous tumor growth and hepatic metastasis, as well as peritoneal carcinomatosis. Importantly, ATF3 expression was lower in human colon cancer specimens, as compared to corresponding normal surrounding tissues, suggesting that ATF3 may represent a down-regulated tumor suppressor in colon cancer. Conclusion In conclusion, ATF3 down-regulation in colon cancer promotes tumor growth and metastasis. Considering that blocking Hsp90 induces ATF3 expression, Hsp90 inhibition may represent a valid strategy to treat metastatic colon cancer by up-regulating this anti-metastatic transcription factor. PMID

  20. Vascular rings.

    PubMed

    Backer, Carl L; Mongé, Michael C; Popescu, Andrada R; Eltayeb, Osama M; Rastatter, Jeffrey C; Rigsby, Cynthia K

    2016-06-01

    The term vascular ring refers to congenital vascular anomalies of the aortic arch system that compress the esophagus and trachea, causing symptoms related to those two structures. The most common vascular rings are double aortic arch and right aortic arch with left ligamentum. Pulmonary artery sling is rare and these patients need to be carefully evaluated for frequently associated tracheal stenosis. Another cause of tracheal compression occurring only in infants is the innominate artery compression syndrome. In the current era, the diagnosis of a vascular ring is best established by CT imaging that can accurately delineate the anatomy of the vascular ring and associated tracheal pathology. For patients with a right aortic arch there recently has been an increased recognition of a structure called a Kommerell diverticulum which may require resection and transfer of the left subclavian artery to the left carotid artery. A very rare vascular ring is the circumflex aorta that is now treated with the aortic uncrossing operation. Patients with vascular rings should all have an echocardiogram because of the incidence of associated congenital heart disease. We also recommend bronchoscopy to assess for additional tracheal pathology and provide an assessment of the degree of tracheomalacia and bronchomalacia. The outcomes of surgical intervention are excellent and most patients have complete resolution of symptoms over a period of time. PMID:27301603

  1. Update on the Argonne positron accumulator ring

    SciTech Connect

    Borland, M.

    1993-07-01

    The injector for the Advanced Photon Source incorporates a 450-MeV positron accumulator ring (PAR) to decrease the filling time with the 2-Hz synchrotron. In addition to accumulating positrons from the linac, the PAR damps the beam and reduces the bunch length. The PAR lattice has been redesigned to use zero-gradient dipoles, while retaining essentially the same damping partition. Extensive simulations have been performed to set tolerances that will give high capture efficiency, in spite of the large momentum spread of the incoming positron beam.

  2. Rings from Close Encounters

    NASA Astrophysics Data System (ADS)

    Kohler, Susanna

    2016-09-01

    the centaur internal structure, its spin, and the distance of closest approach of the centaur to the giant planet. Blue and red represent icy and silicate material, respectively. [Hyodo et al. 2016]The outcomes of the close encounters are diverse, depending strongly on the internal structure and spin of the minor planet and the geometry of the encounter. But the team finds that, in many scenarios, the centaur is only partially destroyed by tidal forces from the giant as it passes close by.In these cases the icy mantle and even some of the centaurs core can be ripped away and scattered, becoming gravitationally bound to the largest remaining clump of the core. The particles travel in highly eccentric orbits, gradually damping as they collide with each other and forming a disk around the remaining core. Further dynamical evolution of this disk could easily shape the rings that we observe today around Chariklo and Chiron.If Hyodo and collaborators scenario is correct, then Chariklo and Chiron are differentiated bodies with dense silicate cores, and their rings are either pure water ice, or a mixture of water ice and a small amount of silicate. Future observations of these minor planets will help to test this model and observations of other centaurs may discover yet more ring systems hiding in our solar system!BonusCheck out this awesomeanimation from ESO showing an artists impression of thering system around Chariklo! [ESO/L. Calada/M. Kornmesser]CitationRyuki Hyodo et al 2016 ApJ 828 L8. doi:10.3847/2041-8205/828/1/L8

  3. The origin of the eccentricities of the rings of Uranus

    NASA Technical Reports Server (NTRS)

    Goldreich, P.; Tremaine, S.

    1981-01-01

    The effect of gravitational perturbations from a nearby satellite on the eccentricity e of a narrow particulate ring is considered. The perturbations near a resonance in an eccentric ring may be divided into corotation and Lindblad terms. For small e, the corotation terms damp e, whereas the Lindblad terms excite e. In the absence of saturation the corotation terms win by a small margin, and e damps. However, if the perturbations open gaps at the strongest resonances, then the Lindblad terms win, and e grows. This result offers an explanation for the existence of both circular and eccentric rings around Uranus. It is also shown that eccentricity changes induced by circular rings on eccentric satellite orbits are similar to those induced by satellites with circular orbits on eccentric rings.

  4. Reconnection of colliding vortex rings.

    PubMed

    Chatelain, Philippe; Kivotides, Demosthenes; Leonard, Anthony

    2003-02-01

    We investigate numerically the Navier-Stokes dynamics of reconnecting vortex rings at small Reynolds number for a variety of configurations. We find that reconnections are dissipative due to the smoothing of vorticity gradients at reconnection kinks and to the formation of secondary structures of stretched antiparallel vorticity which transfer kinetic energy to small scales where it is subsequently dissipated efficiently. In addition, the relaxation of the reconnection kinks excites Kelvin waves which due to strong damping are of low wave number and affect directly only large scale properties of the flow. PMID:12633362

  5. The bZIP repressor proteins, c-Jun dimerization protein 2 and activating transcription factor 3, recruit multiple HDAC members to the ATF3 promoter.

    PubMed

    Darlyuk-Saadon, Ilona; Weidenfeld-Baranboim, Keren; Yokoyama, Kazunari K; Hai, Tsonwin; Aronheim, Ami

    2012-01-01

    JDP2, is a basic leucine zipper (bZIP) protein displaying a high degree of homology with the stress inducible transcription factor, ATF3. Both proteins bind to cAMP and TPA response elements and repress transcription by multiple mechanisms. Histone deacetylases (HDACs) play a key role in gene inactivation by deacetylating lysine residues on histones. Here we describe the association of JDP2 and ATF3 with HDACs 1, 2-6 and 10. Association of HDAC3 and HDAC6 with JDP2 and ATF3 occurs via direct protein-protein interactions. Only part of the N-terminal bZIP motif of JDP2 and ATF3 basic domain is necessary and sufficient for the interaction with HDACs in a manner that is independent of coiled-coil dimerization. Class I HDACs associate with the bZIP repressors via the DAC conserved domain whereas the Class IIb HDAC6 associates through its C-terminal unique binder of ubiquitin Zn finger domain. Both JDP2 and ATF3 are known to bind and repress the ATF3 promoter. MEF cells treated with histone deacetylase inhibitor, trichostatin A (TSA) display enhanced ATF3 transcription. ATF3 enhanced transcription is significantly reduced in MEF cells lacking both ATF3 and JDP2. Collectively, we propose that the recruitment of multiple HDAC members to JDP2 and ATF3 is part of their transcription repression mechanism. PMID:22989952

  6. Assessing the contribution of thrombospondin-4 induction and ATF6α activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction.

    PubMed

    Krawczyk, Katarzyna K; Ekman, Mari; Rippe, Catarina; Grossi, Mario; Nilsson, Bengt-Olof; Albinsson, Sebastian; Uvelius, Bengt; Swärd, Karl

    2016-01-01

    Phenotypic modulation of smooth muscle cells is a hallmark of disease. The associated expansion of endoplasmic reticulum (ER) volume remains unexplained. Thrombospondin-4 was recently found to promote ATF6α activation leading to ER expansion. Using bladder outlet obstruction as a paradigm for phenotypic modulation, we tested if thrombospondin-4 is induced in association with ATF6α activation and ER expansion. Thrombospondin-4 was induced and ATF6α was activated after outlet obstruction in rodents. Increased abundance of spliced of Xbp1, another ER-stress sensor, and induction of Atf4 and Creb3l2 was also seen. Downstream of ATF6α, Calr, Manf, Sdf2l1 and Pdi increased as did ER size, whereas contractile markers were reduced. Overexpression of ATF6α, but not of thrombospondin-4, increased Calr, Manf, Sdf2l1 and Pdi and caused ER expansion, but the contractile markers were inert. Knockout of thrombospondin-4 neither affected bladder growth nor expression of ATF6α target genes, and repression of contractile markers was the same, even if ATF6α activation was curtailed. Increases of Xbp1s, Atf4 and Creb3l2 were similar. Our findings demonstrate reciprocal regulation of the unfolded protein response, including ATF6α activation and ER expansion, and reduced contractile differentiation in bladder outlet obstruction occurring independently of thrombospondin-4, which however is a sensitive indicator of obstruction. PMID:27581066

  7. Assessing the contribution of thrombospondin-4 induction and ATF6α activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction

    PubMed Central

    Krawczyk, Katarzyna K.; Ekman, Mari; Rippe, Catarina; Grossi, Mario; Nilsson, Bengt-Olof; Albinsson, Sebastian; Uvelius, Bengt; Swärd, Karl

    2016-01-01

    Phenotypic modulation of smooth muscle cells is a hallmark of disease. The associated expansion of endoplasmic reticulum (ER) volume remains unexplained. Thrombospondin-4 was recently found to promote ATF6α activation leading to ER expansion. Using bladder outlet obstruction as a paradigm for phenotypic modulation, we tested if thrombospondin-4 is induced in association with ATF6α activation and ER expansion. Thrombospondin-4 was induced and ATF6α was activated after outlet obstruction in rodents. Increased abundance of spliced of Xbp1, another ER-stress sensor, and induction of Atf4 and Creb3l2 was also seen. Downstream of ATF6α, Calr, Manf, Sdf2l1 and Pdi increased as did ER size, whereas contractile markers were reduced. Overexpression of ATF6α, but not of thrombospondin-4, increased Calr, Manf, Sdf2l1 and Pdi and caused ER expansion, but the contractile markers were inert. Knockout of thrombospondin-4 neither affected bladder growth nor expression of ATF6α target genes, and repression of contractile markers was the same, even if ATF6α activation was curtailed. Increases of Xbp1s, Atf4 and Creb3l2 were similar. Our findings demonstrate reciprocal regulation of the unfolded protein response, including ATF6α activation and ER expansion, and reduced contractile differentiation in bladder outlet obstruction occurring independently of thrombospondin-4, which however is a sensitive indicator of obstruction. PMID:27581066

  8. Assembly of a Functional Beta Interferon Enhanceosome Is Dependent on ATF-2–c-jun Heterodimer Orientation

    PubMed Central

    Falvo, James V.; Parekh, Bhavin S.; Lin, Charles H.; Fraenkel, Ernest; Maniatis, Tom

    2000-01-01

    Heterodimeric transcription factors, including the basic region-leucine zipper (bZIP) protein ATF-2–c-jun, are well-characterized components of an enhanceosome that mediates virus induction of the human beta interferon (IFN-β) gene. Here we report that within the IFN-β enhanceosome the ATF-2–c-jun heterodimer binds in a specific orientation, which is required for assembly of a complex between ATF-2–c-jun and interferon regulatory factor 3 (IRF-3). We demonstrate that correct orientation of the ATF-2–c-jun binding site is required for virus induction of the IFN-β gene and for IRF-3-dependent activation of a composite ATF-2– c-jun–IRF site in the IFN-β promoter. We also show that in vitro the DNA-bound ATF-2–c-jun heterodimer adopts a fixed orientation upon the binding of IRF-3 at an adjacent site in the IFN-β enhancer and that the DNA-binding domain of IRF-3 is sufficient to mediate this effect. In addition, we show that the DNA-binding domain of ATF-2 is necessary and sufficient for selective protein-protein interactions with IRF-3. Strikingly, in vivo chromatin immunoprecipitation experiments with IFN-β reporter constructs reveal that recruitment of IRF-3 to the IFN-β promoter upon virus infection is dependent on the orientation of the ATF-2–c-jun heterodimer binding site. These observations demonstrate functional and physical cooperativity between the bZIP and IRF transcription factor families and illustrate the critical role of heterodimeric transcription factors in formation of the IFN-β enhanceosome. PMID:10848607

  9. Timoshenko systems with indefinite damping

    NASA Astrophysics Data System (ADS)

    Muñoz Rivera, Jaime E.; Racke, Reinhard

    2008-05-01

    We consider the Timoshenko system in a bounded domain . The system has an indefinite damping mechanism, i.e. with a damping function a=a(x) possibly changing sign, present only in the equation for the rotation angle. We shall prove that the system is still exponentially stable under the same conditions as in the positive constant damping case, and provided and , for [epsilon] small enough. The decay rate will be described explicitly. In the arguments, we shall also give a new proof of exponential stability for the constant case . Moreover, we give a precise description of the decay rate and demonstrate that the system has the spectrum determined growth (SDG) property, i.e. the type of the induced semigroup coincides with the spectral bound for its generator.

  10. Storage Rings

    SciTech Connect

    Fischer, W.

    2011-01-01

    Storage rings are circular machines that store particle beams at a constant energy. Beams are stored in rings without acceleration for a number of reasons (Tab. 1). Storage rings are used in high-energy, nuclear, atomic, and molecular physics, as well as for experiments in chemistry, material and life sciences. Parameters for storage rings such as particle species, energy, beam intensity, beam size, and store time vary widely depending on the application. The beam must be injected into a storage ring but may not be extracted (Fig. 1). Accelerator rings such as synchrotrons are used as storage rings before and after acceleration. Particles stored in rings include electrons and positrons; muons; protons and anti-protons; neutrons; light and heavy, positive and negative, atomic ions of various charge states; molecular and cluster ions, and neutral polar molecules. Spin polarized beams of electrons, positrons, and protons were stored. The kinetic energy of the stored particles ranges from 10{sup -6} eV to 3.5 x 10{sup 12} eV (LHC, 7 x 10{sup 12} eV planned), the number of stored particles from one (ESR) to 1015 (ISR). To store beam in rings requires bending (dipoles) and transverse focusing (quadrupoles). Higher order multipoles are used to correct chromatic aberrations, to suppress instabilities, and to compensate for nonlinear field errors of dipoles and quadrupoles. Magnetic multipole functions can be combined in magnets. Beams are stored bunched with radio frequency systems, and unbunched. The magnetic lattice and radio frequency system are designed to ensure the stability of transverse and longitudinal motion. New technologies allow for better storage rings. With strong focusing the beam pipe dimensions became much smaller than previously possible. For a given circumference superconducting magnets make higher energies possible, and superconducting radio frequency systems allow for efficient replenishment of synchrotron radiation losses of large current electron or

  11. Landau damping of auroral hiss

    NASA Technical Reports Server (NTRS)

    Morgan, D. D.; Gurnett, D. A.; Menietti, J. D.; Winningham, J. D.; Burch, J. L.

    1994-01-01

    Auroral hiss is observed to propagate over distances comparable to an Earth radius from its source in the auroral oval. The role of Landau damping is investigated for upward propagating auroral hiss. By using a ray tracing code and a simplified model of the distribution function, the effect of Landau damping is calculated for auroral hiss propagation through the environment around the auroral oval. Landau damping is found to be the likely mechanism for explaining some of the one-sided auroral hiss funnels observed by Dynamics Explorer 1. It is also found that Landau damping puts a lower limit on the wavelength of auroral hiss. Poleward of the auroral oval, Landau damping is found in a typical case to limit omega/k(sub parallel) to values of 3.4 x 10(exp 4) km/s or greater, corresponding to resonance energies of 3.2 keV or greater and wavelengths of 2 km or greater. For equatorward propagation, omega/k(sub parallel) is limited to values greater than 6.8 x 10(exp 4) km/s, corresponding to resonance energies greater than 13 keV and wavelengths greater than 3 km. Independent estimates based on measured ratios of the magnetic to electric field intensity also show that omega/k(sub parallel) corresponds to resonance energies greater than 1 keV and wavelengths greater than 1 km. These results lead to the difficulty that upgoing electron beams sufficiently energetic to directly generate auroral hiss of the inferred wavelength are not usually observed. A partial transmission mechanism utilizing density discontinuities oblique to the magnetic field is proposed for converting auroral hiss to wavelengths long enough to avoid damping of the wave over long distances. Numerous reflections of the wave in an upwardly flared density cavity could convert waves to significantly increased wavelengths and resonance velocities.

  12. Landau damping of auroral hiss

    SciTech Connect

    Morgan, D.D.; Gurnett, D.A.; Menietti, J.D.; Winningham, J.D.; Burch, J.L.

    1994-02-01

    Auroral hiss is observed to propagate over distances comparable to an Earth radius from its source in the auroral oval. The role of Landau damping is investigated for upward propagating auroral hiss. By using a ray tracing code and a simplified model of the distribution function, the effect of Landau damping is calculated for auroral hiss propagation through the environment around the auroral oval. Landau damping is found to be the likely mechanism for explaining some of the one-sided auroral hiss funnels observed by Dynamics Explorer 1. It is also found that Landau damping puts a lower limit on the wavelength of auroral hiss. Poleward of the auroral oval, Landau damping is found in a typical case to limit {omega}/k{parallel} to values of 3.4 x 10{sup 4} km/s or greater, corresponding to resonance energies of 3.2 keV or greater and wavelengths of 2 km or greater. For equatorward propagation, {omega}/k{parallel} is limited to values greater than 6.8 x 10{sup 4} km/s, corresponding to resonance energies greater than 13 keV and wavelengths greater than 3 km. Independent estimates based on measured ratios of the magnetic to electric field intensity also show that {omega}/k{parallel} corresponds to resonance energies greater than 1 keV and wavelengths greater than 1 km. These results lead to the difficulty that upgoing electron beams sufficiently energetic to directly generate auroral hiss of the inferred wavelength are not usually observed. A partial transmission mechanism utilizing density discontinuities oblique to the magnetic field is proposed for converting auroral hiss to wavelengths long enough to avoid damping of the wave over long distances. Numerous reflections of the wave in an upwardly flared density cavity could convert waves to significantly increased wavelengths and resonance velocities. 36 refs., 12 figs., 4 tabs.

  13. Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress

    PubMed Central

    D’Osualdo, Andrea; Anania, Veronica G.; Yu, Kebing; Lill, Jennie R.; Kaufman, Randal J.; Matsuzawa, Shu-ichi; Reed, John C.

    2015-01-01

    Perturbation of endoplasmic reticulum (ER) homeostasis triggers the ER stress response (also known as Unfolded Protein Response), a hallmark of many pathological disorders. However the connection between ER stress and inflammation remains largely unexplored. Recent data suggest that ER stress controls the activity of inflammasomes, key signaling platforms that mediate innate immune responses. Here we report that expression of NLRP1, a core inflammasome component, is specifically up-regulated during severe ER stress conditions in human cell lines. Both IRE1α and PERK, but not the ATF6 pathway, modulate NLRP1 gene expression. Furthermore, using mutagenesis, chromatin immunoprecipitation and CRISPR-Cas9-mediated genome editing technology, we demonstrate that ATF4 transcription factor directly binds to NLRP1 promoter during ER stress. Although involved in different types of inflammatory responses, XBP-1 splicing was not required for NLRP1 induction. This study provides further evidence that links ER stress with innate PMID:26086088

  14. Quantitative phase retrieval with picosecond X-ray pulses from the ATF Inverse Compton Scattering source

    SciTech Connect

    Endrizzi, M.; Pogorelsky, I.; Gureyev, T.E.; Delogu, P.; Oliva, P.; Golosio, B.; Carpinelli, M.; Yakimenko, Y.; Bottigli, U.

    2011-01-28

    Quantitative phase retrieval is experimentally demonstrated using the Inverse Compton Scattering X-ray source available at the Accelerator Test Facility (ATF) in the Brookhaven National Laboratory. Phase-contrast images are collected using in-line geometry, with a single X-ray pulse of approximate duration of one picosecond. The projected thickness of homogeneous samples of various polymers is recovered quantitatively from the time-averaged intensity of transmitted X-rays. The data are in good agreement with the expectations showing that ATF Inverse Compton Scattering source is suitable for performing phase-sensitive quantitative X-ray imaging on the picosecond scale. The method shows promise for quantitative imaging of fast dynamic phenomena.

  15. Nutrient shortage triggers the hexosamine biosynthetic pathway via the GCN2-ATF4 signalling pathway

    PubMed Central

    Chaveroux, Cédric; Sarcinelli, Carmen; Barbet, Virginie; Belfeki, Sofiane; Barthelaix, Audrey; Ferraro-Peyret, Carole; Lebecque, Serge; Renno, Toufic; Bruhat, Alain; Fafournoux, Pierre; Manié, Serge N.

    2016-01-01

    The hexosamine biosynthetic pathway (HBP) is a nutrient-sensing metabolic pathway that produces the activated amino sugar UDP-N-acetylglucosamine, a critical substrate for protein glycosylation. Despite its biological significance, little is known about the regulation of HBP flux during nutrient limitation. Here, we report that amino acid or glucose shortage increase GFAT1 production, the first and rate-limiting enzyme of the HBP. GFAT1 is a transcriptional target of the activating transcription factor 4 (ATF4) induced by the GCN2-eIF2α signalling pathway. The increased production of GFAT1 stimulates HBP flux and results in an increase in O-linked β-N-acetylglucosamine protein modifications. Taken together, these findings demonstrate that ATF4 provides a link between nutritional stress and the HBP for the regulation of the O-GlcNAcylation-dependent cellular signalling. PMID:27255611

  16. Nutrient shortage triggers the hexosamine biosynthetic pathway via the GCN2-ATF4 signalling pathway.

    PubMed

    Chaveroux, Cédric; Sarcinelli, Carmen; Barbet, Virginie; Belfeki, Sofiane; Barthelaix, Audrey; Ferraro-Peyret, Carole; Lebecque, Serge; Renno, Toufic; Bruhat, Alain; Fafournoux, Pierre; Manié, Serge N

    2016-01-01

    The hexosamine biosynthetic pathway (HBP) is a nutrient-sensing metabolic pathway that produces the activated amino sugar UDP-N-acetylglucosamine, a critical substrate for protein glycosylation. Despite its biological significance, little is known about the regulation of HBP flux during nutrient limitation. Here, we report that amino acid or glucose shortage increase GFAT1 production, the first and rate-limiting enzyme of the HBP. GFAT1 is a transcriptional target of the activating transcription factor 4 (ATF4) induced by the GCN2-eIF2α signalling pathway. The increased production of GFAT1 stimulates HBP flux and results in an increase in O-linked β-N-acetylglucosamine protein modifications. Taken together, these findings demonstrate that ATF4 provides a link between nutritional stress and the HBP for the regulation of the O-GlcNAcylation-dependent cellular signalling. PMID:27255611

  17. Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress.

    PubMed

    D'Osualdo, Andrea; Anania, Veronica G; Yu, Kebing; Lill, Jennie R; Kaufman, Randal J; Matsuzawa, Shu-ichi; Reed, John C

    2015-01-01

    Perturbation of endoplasmic reticulum (ER) homeostasis triggers the ER stress response (also known as Unfolded Protein Response), a hallmark of many pathological disorders. However the connection between ER stress and inflammation remains largely unexplored. Recent data suggest that ER stress controls the activity of inflammasomes, key signaling platforms that mediate innate immune responses. Here we report that expression of NLRP1, a core inflammasome component, is specifically up-regulated during severe ER stress conditions in human cell lines. Both IRE1α and PERK, but not the ATF6 pathway, modulate NLRP1 gene expression. Furthermore, using mutagenesis, chromatin immunoprecipitation and CRISPR-Cas9-mediated genome editing technology, we demonstrate that ATF4 transcription factor directly binds to NLRP1 promoter during ER stress. Although involved in different types of inflammatory responses, XBP-1 splicing was not required for NLRP1 induction. This study provides further evidence that links ER stress with innate. PMID:26086088

  18. 4D Emittance Measurements Using Multiple Wire and Waist Scan Methods in the ATF Extraction Line

    SciTech Connect

    Rimbault, C.; Bambade, P.; Brossard, J.; Alabau, M.; Kuroda, S.; Scarfe, A.; Woodley, M.; /SLAC

    2011-11-02

    Emittance measurements performed in the diagnostic section of the Accelerator Test Facility (ATF) extraction line since 1998 led to vertical emittances three times larger than the expected ones, with a strong dependence on intensity. An experimental program is pursued to investigate potential sources of emittance growth and find possible remedies. This requires efficient and reliable emittance measurement techniques. In the past, several phase-space reconstruction methods developed at SLAC and KEK have been used to estimate the vertical emittance, based on multiple location beam size measurements and dedicated quadrupole scans. These methods have been shown to be very sensitive to measurement errors and other fluctuations in the beam conditions. In this context new emittance measurements have been performed revisiting these methods and newly developed ones with a systematic approach to compare and characterise their performance in the ATF extraction line.

  19. Damped vacuum states of light

    NASA Astrophysics Data System (ADS)

    Philbin, T. G.

    2016-09-01

    We consider one-dimensional propagation of quantum light in the presence of a block of material, with a full account of dispersion and absorption. The electromagnetic zero-point energy for some frequencies is damped (suppressed) by the block below the free-space value, while for other frequencies it is increased. We also calculate the regularized (Casimir) zero-point energy at each frequency and find that it too is damped below the free-space value (zero) for some frequencies. The total Casimir energy is positive.

  20. Loss of ATF3 promotes hormone-induced prostate carcinogenesis and the emergence of CK5(+)CK8(+) epithelial cells.

    PubMed

    Wang, Z; Kim, J; Teng, Y; Ding, H-F; Zhang, J; Hai, T; Cowell, J K; Yan, C

    2016-07-01

    Steroid sex hormones can induce prostate carcinogenesis, and are thought to contribute to the development of prostate cancer during aging. However, the mechanism for hormone-induced prostate carcinogenesis remains elusive. Here, we report that activating transcription factor 3 (ATF3)-a broad stress sensor-suppressed hormone-induced prostate carcinogenesis in mice. Although implantation of testosterone and estradiol (T+E2) pellets for 2 months in wild-type mice rarely induced prostatic intraepithelial neoplasia (PIN) in dorsal prostates (one out of eight mice), the loss of ATF3 led to the appearance of not only PIN but also invasive lesions in almost all examined animals. The enhanced carcinogenic effects of hormones on ATF3-deficient prostates did not appear to be caused by a change in estrogen signaling, but were more likely a consequence of elevated androgen signaling that stimulated differentiation of prostatic basal cells into transformation-preferable luminal cells. Indeed, we found that hormone-induced lesions in ATF3-knockout mice often contained cells with both basal and luminal characteristics, such as p63(+) cells (a basal-cell marker) showing luminal-like morphology, or cells double-stained with basal (CK5(+)) and luminal (CK8(+)) markers. Consistent with these findings, low ATF3 expression was found to be a poor prognostic marker for prostate cancer in a cohort of 245 patients. Our results thus support that ATF3 is a tumor suppressor in prostate cancer. PMID:26522727

  1. Regression/eradication of gliomas in mice by a systemically-deliverable ATF5 dominant-negative peptide.

    PubMed

    Cates, Charles C; Arias, Angelo D; Nakayama Wong, Lynn S; Lamé, Michael W; Sidorov, Maxim; Cayanan, Geraldine; Rowland, Douglas J; Fung, Jennifer; Karpel-Massler, Georg; Siegelin, Markus D; Greene, Lloyd A; Angelastro, James M

    2016-03-15

    Malignant gliomas have poor prognosis and urgently require new therapies. Activating Transcription Factor 5 (ATF5) is highly expressed in gliomas, and interference with its expression/function precipitates targeted glioma cell apoptosis in vitro and in vivo. We designed a novel deliverable truncated-dominant-negative (d/n) form of ATF5 fused to a cell-penetrating domain (Pen-d/n-ATF5-RP) that can be intraperitoneally/subcutaneously administered to mice harboring malignant gliomas generated; (1) by PDGF-B/sh-p53 retroviral transformation of endogenous neural progenitor cells; and (2) by human U87-MG xenografts. In vitro Pen-d/n-ATF5-RP entered into glioma cells and triggered massive apoptosis. In vivo, subcutaneously-administered Pen-d/n-ATF5-RP passed the blood brain barrier, entered normal brain and tumor cells, and then caused rapid selective tumor cell death. MRI verified elimination of retrovirus-induced gliomas within 8-21 days. Histopathology revealed growth-suppression of intracerebral human U87-MG cells xenografts. For endogenous PDGF-B gliomas, there was no recurrence or mortality at 6-12 months versus 66% mortality in controls at 6 months. Necropsy and liver-kidney blood enzyme analysis revealed no adverse effects on brain or other tissues. Our findings thus identify Pen-d/n-ATF5-RP as a potential therapy for malignant gliomas. PMID:26863637

  2. Ceapins are a new class of unfolded protein response inhibitors, selectively targeting the ATF6α branch

    PubMed Central

    Gallagher, Ciara M; Garri, Carolina; Cain, Erica L; Ang, Kenny Kean-Hooi; Wilson, Christopher G; Chen, Steven; Hearn, Brian R; Jaishankar, Priyadarshini; Aranda-Diaz, Andres; Arkin, Michelle R; Renslo, Adam R; Walter, Peter

    2016-01-01

    The membrane-bound transcription factor ATF6α plays a cytoprotective role in the unfolded protein response (UPR), required for cells to survive ER stress. Activation of ATF6α promotes cell survival in cancer models. We used cell-based screens to discover and develop Ceapins, a class of pyrazole amides, that block ATF6α signaling in response to ER stress. Ceapins sensitize cells to ER stress without impacting viability of unstressed cells. Ceapins are highly specific inhibitors of ATF6α signaling, not affecting signaling through the other branches of the UPR, or proteolytic processing of its close homolog ATF6β or SREBP (a cholesterol-regulated transcription factor), both activated by the same proteases. Ceapins are first-in-class inhibitors that can be used to explore both the mechanism of activation of ATF6α and its role in pathological settings. The discovery of Ceapins now enables pharmacological modulation all three UPR branches either singly or in combination. DOI: http://dx.doi.org/10.7554/eLife.11878.001 PMID:27435960

  3. Unfolded protein response in hypothalamic cultures of wild-type and ATF6α-knockout mice.

    PubMed

    Lu, Wenjun; Hagiwara, Daisuke; Morishita, Yoshiaki; Tochiya, Masayoshi; Azuma, Yoshinori; Suga, Hidetaka; Goto, Motomitsu; Banno, Ryoichi; Sugimura, Yoshihisa; Oyadomari, Seiichi; Mori, Kazutoshi; Arima, Hiroshi

    2016-01-26

    Recent studies suggest that endoplasmic reticulum (ER) stress in the hypothalamus could affect systemic homeostatic regulation in areas such as energy and water balance. Activating transcription factor 6α (ATF6α) is an ER stress transducer which increases the expression of ER chaperones and ER-associated degradation (ERAD) components under ER stress. In the present study, we examined the regulation of the unfolding protein response (UPR) in mouse hypothalamic cultures of wild-type (WT) and ATF6α(-/-) mice. Thapsigargin (TG), an ER stressor, significantly increased the mRNA expression of immunoglobulin heavy chain binding protein (BiP), spliced X-box binding protein 1 (XBP1), activating transcription factor 4 (ATF4), C/EBP homologous protein (CHOP), and ERAD components, in hypothalamic cultures of WT mice with the same threshold (0.1μM) and similar time courses. On the other hand, TG-induced upregulation of BiP and CHOP as well as most ERAD-related genes, but not spliced XBP1 or ATF4, was attenuated in ATF6α(-/-) mice compared with WT mice. Our data suggest that all the UPR arms are activated similarly in the mouse hypothalamus under ER stress conditions, where ATF6α regulates the expression of ER chaperones, CHOP, and ERAD components. PMID:26708632

  4. Ceapins are a new class of unfolded protein response inhibitors, selectively targeting the ATF6α branch.

    PubMed

    Gallagher, Ciara M; Garri, Carolina; Cain, Erica L; Ang, Kenny Kean-Hooi; Wilson, Christopher G; Chen, Steven; Hearn, Brian R; Jaishankar, Priyadarshini; Aranda-Diaz, Andres; Arkin, Michelle R; Renslo, Adam R; Walter, Peter

    2016-01-01

    The membrane-bound transcription factor ATF6α plays a cytoprotective role in the unfolded protein response (UPR), required for cells to survive ER stress. Activation of ATF6α promotes cell survival in cancer models. We used cell-based screens to discover and develop Ceapins, a class of pyrazole amides, that block ATF6α signaling in response to ER stress. Ceapins sensitize cells to ER stress without impacting viability of unstressed cells. Ceapins are highly specific inhibitors of ATF6α signaling, not affecting signaling through the other branches of the UPR, or proteolytic processing of its close homolog ATF6β or SREBP (a cholesterol-regulated transcription factor), both activated by the same proteases. Ceapins are first-in-class inhibitors that can be used to explore both the mechanism of activation of ATF6α and its role in pathological settings. The discovery of Ceapins now enables pharmacological modulation all three UPR branches either singly or in combination. PMID:27435960

  5. Regression/Eradication of gliomas in mice by a systemically-deliverable ATF5 dominant-negative peptide

    PubMed Central

    Cates, Charles C.; Arias, Angelo D.; Wong, Lynn S. Nakayama; Lamé, Michael W.; Sidorov, Maxim; Cayanan, Geraldine; Rowland, Douglas J.; Fung, Jennifer; Karpel-Massler, Georg; Siegelin, Markus D.; Greene, Lloyd A.; Angelastro, James M.

    2016-01-01

    Malignant gliomas have poor prognosis and urgently require new therapies. Activating Transcription Factor 5 (ATF5) is highly expressed in gliomas, and interference with its expression/function precipitates targeted glioma cell apoptosis in vitro and in vivo. We designed a novel deliverable truncated-dominant-negative (d/n) form of ATF5 fused to a cell-penetrating domain (Pen-d/n-ATF5-RP) that can be intraperitoneally/subcutaneously administered to mice harboring malignant gliomas generated; (1) by PDGF-B/sh-p53 retroviral transformation of endogenous neural progenitor cells; and (2) by human U87-MG xenografts. In vitro Pen-d/n-ATF5-RP entered into glioma cells and triggered massive apoptosis. In vivo, subcutaneously-administered Pen-d/n-ATF5-RP passed the blood brain barrier, entered normal brain and tumor cells, and then caused rapid selective tumor cell death. MRI verified elimination of retrovirus-induced gliomas within 8-21 days. Histopathology revealed growth-suppression of intracerebral human U87-MG cells xenografts. For endogenous PDGF-B gliomas, there was no recurrence or mortality at 6-12 months versus 66% mortality in controls at 6 months. Necropsy and liver-kidney blood enzyme analysis revealed no adverse effects on brain or other tissues. Our findings thus identify Pen-d/n-ATF5-RP as a potential therapy for malignant gliomas. PMID:26863637

  6. Structural damping studies at cryogenic temperatures

    NASA Technical Reports Server (NTRS)

    Young, Clarence P., Jr.; Buehrle, Ralph D.

    1994-01-01

    Results of an engineering study to measure changes in structural damping properties of two cryogenic wind tunnel model systems and two metallic test specimens at cryogenic temperatures are presented. Data are presented which indicate overall, a trend toward reduced structural damping at cryogenic temperatures (-250 degrees F) when compared with room temperature damping properties. The study was focused on structures and materials used for model systems tested in the National Transonic Facility (NTF). The study suggests that the significant reductions in damping at extremely cold temperatures are most likely associated with changes in mechanical joint compliance damping rather than changes in material (solid) damping.

  7. The data acquisition and control system for Thomson Scattering on ATF (Advanced Toroidal Facility)

    SciTech Connect

    Stewart, K.A.; Kindsfather, R.R.; Rasmussen, D.A.

    1989-01-01

    The 2-dimensional Thomson Scattering System measuring electron temperatures and densities in the Advanced Toroidal Facility (ATF) is interfaced to a VAX-8700 computer system running in a clustered configuration. Calibration, alignment, and operation of this diagnostic is under computer control. Extensive CAMAC instrumentation is used for timing control, data acquisition, and laser alignment. This paper will discuss the computer hardware and software, system operations, and data storage and retrieval. 3 refs.

  8. FODO-Supercell Based Compact Ring Design with Tunable Momentum Compaction and Optimized Dynamic Aperture

    SciTech Connect

    Sun, Yipeng; /SLAC

    2012-05-11

    A storage ring with tunable momentum compaction has the advantage in achieving different RMS bunch length with similar RF capacity, which is potentially useful for many applications, such as linear collider damping ring and pre-damping ring where injected beam has a large energy spread and a large transverse emittance. A tunable bunch length also makes the commissioning and fine tuning easier in manipulating the single bunch instabilities. In this paper, a compact ring design based on a supercell is presented, which achieves a tunable momentum compaction while maintaining a large dynamic aperture.

  9. Farnesol activates the intrinsic pathway of apoptosis and the ATF4-ATF3-CHOP cascade of ER stress in human T lymphoblastic leukemia Molt4 cells.

    PubMed

    Joo, Joung Hyuck; Ueda, Eiichiro; Bortner, Carl D; Yang, Xiao-Ping; Liao, Grace; Jetten, Anton M

    2015-10-01

    In this study, we demonstrate that treatment of T lymphoblastic leukemic Molt4 cells with farnesol activates the apoptosome via the intrinsic pathway of apoptosis. This induction was associated with changes in the level of intracellular potassium and calcium, the dissipation of the mitochondrial and plasma membrane potential, release of cytochrome c, activation of several caspases, and PARP cleavage. The induction of apoptosis by farnesol was inhibited by the addition of the pan-caspase inhibitor Z-VAD-fmk and by the exogenous expression of the anti-apoptotic protein Bcl2. Analysis of the gene expression profiles by microarray analysis revealed that farnesol increased the expression of several genes related to the unfolded protein response (UPR), including CHOP and CHAC1. This induction was associated with the activation of the PERK-eIF2α-ATF3/4 cascade, but not the XBP-1 branch of the UPR. Although farnesol induced activation of the ERK1/2, p38, and JNK pathways, inhibition of these MAPKs had little effect on farnesol-induced apoptosis or the induction of UPR-related genes. Our data indicate that the induction of apoptosis in leukemic cells by farnesol is mediated through a pathway that involves activation of the apoptosome via the intrinsic pathway and induction of the PERK-eIF2α-ATF3/4 cascade in a manner that is independent of the farnesol-induced activation of MAPKs. PMID:26275811

  10. Molecular pathway of near-infrared laser phototoxicity involves ATF-4 orchestrated ER stress.

    PubMed

    Khan, Imran; Tang, Elieza; Arany, Praveen

    2015-01-01

    High power lasers are used extensively in medicine while lower power applications are popular for optical imaging, optogenetics, skin rejuvenation and a therapeutic modality termed photobiomodulation (PBM). This study addresses the therapeutic dose limits, biological safety and molecular pathway of near-infrared (NIR) laser phototoxicity. Increased erythema and tissue damage were noted in mice skin and cytotoxicity in cell cultures at phototoxic laser doses involving generation of reactive oxygen species (ROS) coupled with a rise in surface temperature (>45 °C). NIR laser phototoxicity results from Activating Transcription Factor-4 (ATF-4) mediated endoplasmic reticulum stress and autophagy. Neutralizations of heat or ROS and overexpressing ATF-4 were noted to rescue NIR laser phototoxicity. Further, NIR laser mediated phototoxicity was noted to be non-genotoxic and non-mutagenic. This study outlines the mechanism of NIR laser phototoxicity and the utility of monitoring surface temperature and ATF4 expression as potential biomarkers to develop safe and effective clinical applications. PMID:26030745

  11. Molecular pathway of near-infrared laser phototoxicity involves ATF-4 orchestrated ER stress

    PubMed Central

    Khan, Imran; Tang, Elieza; Arany, Praveen

    2015-01-01

    High power lasers are used extensively in medicine while lower power applications are popular for optical imaging, optogenetics, skin rejuvenation and a therapeutic modality termed photobiomodulation (PBM). This study addresses the therapeutic dose limits, biological safety and molecular pathway of near-infrared (NIR) laser phototoxicity. Increased erythema and tissue damage were noted in mice skin and cytotoxicity in cell cultures at phototoxic laser doses involving generation of reactive oxygen species (ROS) coupled with a rise in surface temperature (>45 °C). NIR laser phototoxicity results from Activating Transcription Factor-4 (ATF-4) mediated endoplasmic reticulum stress and autophagy. Neutralizations of heat or ROS and overexpressing ATF-4 were noted to rescue NIR laser phototoxicity. Further, NIR laser mediated phototoxicity was noted to be non-genotoxic and non-mutagenic. This study outlines the mechanism of NIR laser phototoxicity and the utility of monitoring surface temperature and ATF4 expression as potential biomarkers to develop safe and effective clinical applications. PMID:26030745

  12. Initial high-power testing of the ATF (Advanced Toroidal Facility) ECH (electron cyclotron heating) system

    SciTech Connect

    White, T.L.; Bigelow, T.S.; Kimrey, H.D. Jr.

    1987-01-01

    The Advanced Toroidal Facility (ATF) is a moderate aspect ratio torsatron that will utilize 53.2 GHz 200 kW Electron Cyclotron Heating (ECH) to produce nearly current-free target plasmas suitable for subsequent heating by strong neutral beam injection. The initial configuration of the ECH system from the gyrotron to ATF consists of an optical arc detector, three bellows, a waveguide mode analyzer, two TiO/sub 2/ mode absorbers, two 90/sup 0/ miter bends, two waveguide pumpouts, an insulating break, a gate valve, and miscellaneous straight waveguide sections feeding a launcher radiating in the TE/sub 02/ mode. Later, a focusing Vlasov launcher will be added to beam the ECH power to the saddle point in ATF magnetic geometry for optimum power deposition. The ECH system has several unique features; namely, the entire ECH system is evacuated, the ECH system is broadband, forward power is monitored by a newly developed waveguide mode analyzer, phase correcting miter bends will be employed, and the ECH system will be capable of operating short pulse to cw. Initial high-power tests show that the overall system efficiency is 87%. The waveguide mode analyzer shows that the gyrotron mode output consists of 13% TE/sub 01/, 82.6% TE/sub 02/, 2.5% TE/sub 03/, and 1.9% TE/sub 04/. 4 refs.

  13. Grass carp (Ctenopharyngodon idella) ATF6 (activating transcription factor 6) modulates the transcriptional level of GRP78 and GRP94 in CIK cells.

    PubMed

    Wang, Xiangqin; Zhang, Tao; Mao, Huiling; Mi, Yichuan; Zhong, Bin; Wei, Lili; Liu, Xiancheng; Hu, Chengyu

    2016-05-01

    ATF transcription factors are stress proteins containing alkaline area-leucine zipper and play an important role in endoplasmic reticulum stress. ATF6 is a protective protein which regulates the adaptation of cells to ER stress by modulating the transcription of UPR (Unfolded Protein Response) target genes, including GRP78 and GRP94. In the present study, a grass carp (Ctenopharyngodon idella) ATF6 full-length cDNA (named CiATF6, KT279356) has been cloned and identified. CiATF6 is 4176 bp in length, comprising 159 nucleotides of 5'-untranslated sequence, a 1947 nucleotides open reading frame and 2170 nucleotides of 3'-untranslated sequences. The largest open reading frame of CiATF6 translates into 648 aa with a typical DNA binding domain (BRLZ domain) and shares significant homology to the known ATF6 counterparts. Phylogenetic reconstruction confirmed its closer evolutionary relationship with other fish counterparts, especially with Zebrafish ATF6. RT-PCR showed that CiATF6 was ubiquitously expressed and significantly up-regulated after stimulation with thermal stress in all tested grass carp tissues. In order to know more about the role of CiATF6 in ER stress, recombinant CiATF6N with His-tag was over-expressed in Rosetta Escherichia coli, and the expressed protein was purified by affinity chromatography with Ni-NTA His-Bind Resin. In vitro, gel mobility shift assays were employed to analyze the interaction of CiATF6 protein with the promoters of grass carp GRP78 and GRP94, respectively. The result has shown that CiATF6 could bind to these promoters with high affinity by means of its BRLZ mainly. To further study the transcriptional regulatory mechanism of CiATF6, Dual-luciferase reporter assays were applied. Recombinant plasmids of pGL3-GRP78P and pGL3-CiGRP94P were constructed and transiently co-transfected with pcDNA3.1-CiATF6 (pcDN3.1-CiATF6-nBRLZ, respectively) into C. idella kidney (CIK) cells. The result has shown that CiATF6 could activate CiGRP78 and

  14. Ring Galaxies

    NASA Astrophysics Data System (ADS)

    Dennefeld, M.; Materne, J.

    1980-09-01

    Among the 338 exotic, intriguing and/or fascinating objects contained in Arp's catalogue of peculiar galaxies, two, Arp 146 and 147, are calling special attention as a presumably separate class of objects displaying closed rings with almost empty interior. It is difficult to find out when, historically speaking, attention was called first to this type of object as a peculiar class, but certainly ga1axies with rings were widely found and recognized in the early sixties, ul}der others by Vorontsov-Velyaminov (1960), Sandage (1961) in the Hubble Atlas or de Vaucouleurs (1964) in the first reference catalogue of ga1axies. The most recent estimates by Arp and Madore (1977) from a search on about 200 Schmidt plates covering 7,000 square degrees give 3.6 per cent of ring galaxies among 2,784 peculiar galaxies found. However, despite the mythological perfection associated with a circle, some ordering is necessary before trying to understand the nature of such objects. This is particularly true because a large fraction of those galaxies with rings are probably normal spiral galaxies of type RS or S(r) as defined by de Vaucouleurs, where the spiral arms are simply "closing the circle". A good example of such "ordinary" galaxy is NGC 3081 in the Hubble Atlas .

  15. A Compact Ring Design with Tunable Momentum Compaction

    SciTech Connect

    Sun, Y.; /SLAC

    2012-05-17

    A storage ring with tunable momentum compaction has the advantage in achieving different RMS bunch length with similar RF capacity, which is potentially useful for many applications, such as linear collider damping ring and predamping ring where injected beam has a large energy spread and a large transverse emittance. A tunable bunch length also makes the commissioning and fine tuning easier in manipulating the single bunch instabilities. In this paper, a compact ring design based on a supercell is presented, which achieves a tunable momentum compaction while maintaining a large dynamic aperture.

  16. Dealing with damping-off

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Damping-off is a common disease that rots and kills both seeds and recently germinated seedlings. The disease is caused by number of different soilborne pathogens, including true fungi (Botrytis, Fusarium, and Rhizoctonia species) and oomycetes (Phytophthora and Pythium species). The seedlings of mo...

  17. Red cell DAMPs and inflammation.

    PubMed

    Mendonça, Rafaela; Silveira, Angélica A A; Conran, Nicola

    2016-09-01

    Intravascular hemolysis, or the destruction of red blood cells in the circulation, can occur in numerous diseases, including the acquired hemolytic anemias, sickle cell disease and β-thalassemia, as well as during some transfusion reactions, preeclampsia and infections, such as those caused by malaria or Clostridium perfringens. Hemolysis results in the release of large quantities of red cell damage-associated molecular patterns (DAMPs) into the circulation, which, if not neutralized by innate protective mechanisms, have the potential to activate multiple inflammatory pathways. One of the major red cell DAMPs, heme, is able to activate converging inflammatory pathways, such as toll-like receptor signaling, neutrophil extracellular trap formation and inflammasome formation, suggesting that this DAMP both activates and amplifies inflammation. Other potent DAMPs that may be released by the erythrocytes upon their rupture include heat shock proteins (Hsp), such as Hsp70, interleukin-33 and Adenosine 5' triphosphate. As such, hemolysis represents a major inflammatory mechanism that potentially contributes to the clinical manifestations that have been associated with the hemolytic diseases, such as pulmonary hypertension and leg ulcers, and likely plays a role in specific complications of sickle cell disease such as endothelial activation, vaso-occlusive processes and tissue injury. PMID:27251171

  18. Damped Oscillator with Delta-Kicked Frequency

    NASA Technical Reports Server (NTRS)

    Manko, O. V.

    1996-01-01

    Exact solutions of the Schrodinger equation for quantum damped oscillator subject to frequency delta-kick describing squeezed states are obtained. The cases of strong, intermediate, and weak damping are investigated.

  19. The partially filled viscous ring damper.

    NASA Technical Reports Server (NTRS)

    Alfriend, K. T.

    1973-01-01

    The problem of a spinning satellite with a partially filled viscous ring damper is investigated. It is shown that there are two distinct modes of motion, the nutation-synchronous mode and spin-synchronous mode. From an approximate solution of the equations of motion a time constant is obtained for each mode. From a consideration of the fluid dynamics several methods are developed for determining the damping constant.

  20. Squeezed states of damped oscillator chain

    NASA Technical Reports Server (NTRS)

    Manko, O. V.

    1993-01-01

    The Caldirola-Kanai model of one-dimensional damped oscillator is extended to the chain of coupled parametric oscillators with damping. The correlated and squeezed states for the chain of coupled parametric oscillators with damping are constructed. Based on the concept of the integrals of motion, it is demonstrated how squeezing phenomenon arises due to parametric excitation.

  1. Upregulated ATF6 contributes to chronic intermittent hypoxia-afforded protection against myocardial ischemia/reperfusion injury.

    PubMed

    Jia, Weikun; Jian, Zhao; Li, Jingwei; Luo, Lin; Zhao, Liang; Zhou, Yang; Tang, Fuqin; Xiao, Yingbin

    2016-05-01

    In the present study, we investigated the role of activating transcription factor 6 (ATF6) in the mechanism by which chronic intermittent hypoxia (CIH) increases tolerance to myocardial ischemia/reperfusion (I/R). Experiments were conducted using a rat model of I/R injury in vivo and isolated Langendorff-perfused rat hearts ex vivo. The role of Akt in this process was also investigated in vitro using rat myoblast H9c2 cells. Cell viability was measured using a cell counting kit-8 assay. Lactate dehydrogenase (LDH) and creatine kinase cardiac isoenzyme activity were also measured as markers of cellular damage. ATF6, Akt and phosphorylated (p)-Akt expression was analyzed by western blot analysis. RNA interference (RNAi) was used to suppress ATF6 expression. We noted that ATF6 expression in the ventricular myocardium was significantly increased in rats exposed to CIH. Furthermore, we noted that CIH preserved cardiac function after I/R in vivo and improved post-ischemic recovery of myocardial performance in isolated rat hearts. ATF6 and p-Akt expression was upregulated in cultured H9c2 cells exposed to chronic mild hypoxia compared with those cultured under normoxic conditions. Chronic mild hypoxia attenuated subsequent simulated I/R injury in H9c2 cells (48 h), as evidenced by increased cell viability and decreased LDH activity. By contrast, decreased cell viability and increased LDH activity were observed in siRNA-ATF6-transfected H9c2 cells, with a concomitant reduction in p-Akt levels. These results indicated that ATF6 upregulation is involved in the mechanism by which CIH attenuates myocardial I/R injury, possibly through upregulation of p-Akt, which is a key regulator of cardiomyocyte survival. PMID:27035093

  2. Characterization of the Enzymatic Activity of SETDB1 and Its 1:1 Complex with ATF7IP.

    PubMed

    Basavapathruni, Aravind; Gureasko, Jodi; Porter Scott, Margaret; Hermans, William; Godbole, Adarsh; Leland, Peter A; Boriack-Sjodin, P Ann; Wigle, Tim J; Copeland, Robert A; Riera, Thomas V

    2016-03-22

    The protein methyltransferase (PMT) SETDB1 is a strong candidate oncogene in melanoma and lung carcinomas. SETDB1 methylates lysine 9 of histone 3 (H3K9), utilizing S-adenosylmethionine (SAM) as the methyl donor and its catalytic activity, has been reported to be regulated by a partner protein ATF7IP. Here, we examine the contribution of ATF7IP to the in vitro activity and substrate specificity of SETDB1. SETDB1 and ATF7IP were co-expressed and 1:1 stoichiometric complexes were purified for comparison against SETDB1 enzyme alone. We employed both radiometric flashplate-based and SAMDI mass spectrometry assays to follow methylation on histone H3 15-mer peptides, where lysine 9 was either unmodified, monomethylated, or dimethylated. Results show that SETDB1 and the SETDB1:ATF7IP complex efficiently catalyze both monomethylation and dimethylation of H3K9 peptide substrates. The activity of the binary complex was 4-fold lower than SETDB1 alone. This difference was due to a decrease in the value of kcat as the substrate KM values were comparable between SETDB1 and the SETDB1:ATF7IP complex. H3K9 methylation by SETDB1 occurred in a distributive manner, and this too was unaffected by the presence of ATF7IP. This finding is important as H3K9 can be methylated by HMTs other than SETDB1 and a distributive mechanism would allow for interplay between multiple HMTs on H3K9. Our results indicate that ATF7IP does not directly modulate SETDB1 catalytic activity, suggesting alternate roles, such as affecting cellular localization or mediating interaction with additional binding partners. PMID:26813693

  3. Intranasal administration of aTf protects and repairs the neonatal white matter after a cerebral hypoxic-ischemic event.

    PubMed

    Guardia Clausi, Mariano; Paez, Pablo M; Campagnoni, Anthony T; Pasquini, Laura A; Pasquini, Juana M

    2012-10-01

    Our previous studies showed that the intracerebral injection of apotransferrin (aTf) attenuates white matter damage and accelerates the remyelination process in a neonatal rat model of cerebral hypoxia-ischemia (HI) injury. However, the intracerebral injection of aTf might not be practical for clinical treatments. Therefore, the development of less invasive techniques capable of delivering aTf to the central nervous system would clearly aid in its effective clinical use. In this work, we have determined whether intranasal (iN) administration of human aTf provides neuroprotection to the neonatal mouse brain following a cerebral hypoxic-ischemic event. Apotransferrin was infused into the naris of neonatal mice and the HI insult was induced by right common carotid artery ligation followed by exposure to low oxygen concentration. Our results showed that aTf was successfully delivered into the neonatal HI brain and detected in the olfactory bulb, forebrain and posterior brain 30 min after inhalation. This treatment successfully reduced white matter damage, neuronal loss and astrogliosis in different brain regions and enhanced the proliferation and survival of oligodendroglial progenitor cells (OPCs) in the subventricular zone and corpus callosum (CC). Additionally, using an in vitro hypoxic model, we demonstrated that aTf prevents oligodendrocyte progenitor cell death by promoting their differentiation. In summary, these data suggest that iN administration of aTf has the potential to be used for clinical treatment to protect myelin and to induce remyelination in demyelinating hypoxic-ischemic events in the neonatal brain. PMID:22736466

  4. Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.

    PubMed

    Nimmanee, Panjaphorn; Woo, Patrick C Y; Vanittanakom, Pramote; Youngchim, Sirida; Vanittanakom, Nongnuch

    2014-01-01

    Penicillium marneffei, the pathogenic thermal dimorphic fungus is a causative agent of a fatal systemic disease, penicilliosis marneffei, in immunocompromised patients especially HIV patients. For growth and survival, this fungus has to adapt to environmental stresses outside and inside host cells and this adaptation requires stress signaling pathways and regulation of gene expression under various kinds of stresses. In this report, P. marneffei activating transcription factor (atfA) gene encoding bZip-type transcription factor was characterized. To determine functions of this gene, atfA isogenic mutant strain was constructed using the modified split marker recombination method. The phenotypes and susceptibility to varieties of stresses including osmotic, oxidative, heat, UV, cell wall and cell membrane stresses of the mutant strain were compared with the wild type and the atfA complemented strains. Results demonstrated that the mRNA expression level of P. marneffei atfA gene increased under heat stress at 42°C. The atfA mutant was more sensitive to sodium dodecyl sulphate, amphotericin B and tert-butyl hydroperoxide than the wild type and complemented strains but not hydrogen peroxide, menadione, NaCl, sorbitol, calcofluor white, itraconazole, UV stresses and heat stress at 39°C. In addition, recovery of atfA mutant conidia after mouse and human macrophage infections was significantly decreased compared to those of wild type and complemented strains. These results indicated that the atfA gene was required by P. marneffei under specific stress conditions and might be necessary for fighting against host immune cells during the initiation of infection. PMID:25365258

  5. Upregulated ATF6 contributes to chronic intermittent hypoxia-afforded protection against myocardial ischemia/reperfusion injury

    PubMed Central

    JIA, WEIKUN; JIAN, ZHAO; LI, JINGWEI; LUO, LIN; ZHAO, LIANG; ZHOU, YANG; TANG, FUQIN; XIAO, YINGBIN

    2016-01-01

    In the present study, we investigated the role of activating transcription factor 6 (ATF6) in the mechanism by which chronic intermittent hypoxia (CIH) increases tolerance to myocardial ischemia/reperfusion (I/R). Experiments were conducted using a rat model of I/R injury in vivo and isolated Langendorff-perfused rat hearts ex vivo. The role of Akt in this process was also investigated in vitro using rat myoblast H9c2 cells. Cell viability was measured using a cell counting kit-8 assay. Lactate dehydrogenase (LDH) and creatine kinase cardiac isoenzyme activity were also measured as markers of cellular damage. ATF6, Akt and phosphorylated (p)-Akt expression was analyzed by western blot analysis. RNA interference (RNAi) was used to suppress ATF6 expression. We noted that ATF6 expression in the ventricular myocardium was significantly increased in rats exposed to CIH. Furthermore, we noted that CIH preserved cardiac function after I/R in vivo and improved post-ischemic recovery of myocardial performance in isolated rat hearts. ATF6 and p-Akt expression was upregulated in cultured H9c2 cells exposed to chronic mild hypoxia compared with those cultured under normoxic conditions. Chronic mild hypoxia attenuated subsequent simulated I/R injury in H9c2 cells (48 h), as evidenced by increased cell viability and decreased LDH activity. By contrast, decreased cell viability and increased LDH activity were observed in siRNA-ATF6-transfected H9c2 cells, with a concomitant reduction in p-Akt levels. These results indicated that ATF6 upregulation is involved in the mechanism by which CIH attenuates myocardial I/R injury, possibly through upregulation of p-Akt, which is a key regulator of cardiomyocyte survival. PMID:27035093

  6. Central activating transcription factor 4 (ATF4) regulates hepatic insulin resistance in mice via S6K1 signaling and the vagus nerve.

    PubMed

    Zhang, Qian; Yu, Junjie; Liu, Bin; Lv, Ziquan; Xia, Tingting; Xiao, Fei; Chen, Shanghai; Guo, Feifan

    2013-07-01

    Recent studies have revealed that the central nervous system, particularly the hypothalamus, is critical for regulating insulin sensitivity in peripheral tissues. The aim of our current study is to investigate the possible involvement of hypothalamic activating transcription factor 4 (ATF4) in the regulation of insulin sensitivity in the liver. Here, we show that overexpression of ATF4 in the hypothalamus resulting from intracerebroventricular injection of adenovirus expressing ATF4 induces hepatic insulin resistance in mice and that inhibition of hypothalamic ATF4 by intracerebroventricular adenovirus expressing a dominant-negative ATF4 variant has the opposite effect. We also show that hypothalamic ATF4-induced insulin resistance is significantly blocked by selective hepatic vagotomy or by inhibiting activity of the mammalian target of rapamycin (mTOR) downstream target S6K1. Finally, we show that inhibition of hypothalamic ATF4 reverses hepatic insulin resistance induced by acute brain endoplasmic reticulum (ER) stress. Taken together, our study describes a novel central pathway regulating hepatic insulin sensitivity that is mediated by hypothalamic ATF4/mTOR/S6K1 signaling and the vagus nerve and demonstrates an important role for hypothalamic ATF4 in brain ER stress-induced hepatic insulin resistance. These results may lead to the identification of novel therapeutic targets for treating insulin resistance and associated metabolic diseases. PMID:23454693

  7. Central Activating Transcription Factor 4 (ATF4) Regulates Hepatic Insulin Resistance in Mice via S6K1 Signaling and the Vagus Nerve

    PubMed Central

    Zhang, Qian; Yu, Junjie; Liu, Bin; Lv, Ziquan; Xia, Tingting; Xiao, Fei; Chen, Shanghai; Guo, Feifan

    2013-01-01

    Recent studies have revealed that the central nervous system, particularly the hypothalamus, is critical for regulating insulin sensitivity in peripheral tissues. The aim of our current study is to investigate the possible involvement of hypothalamic activating transcription factor 4 (ATF4) in the regulation of insulin sensitivity in the liver. Here, we show that overexpression of ATF4 in the hypothalamus resulting from intracerebroventricular injection of adenovirus expressing ATF4 induces hepatic insulin resistance in mice and that inhibition of hypothalamic ATF4 by intracerebroventricular adenovirus expressing a dominant-negative ATF4 variant has the opposite effect. We also show that hypothalamic ATF4-induced insulin resistance is significantly blocked by selective hepatic vagotomy or by inhibiting activity of the mammalian target of rapamycin (mTOR) downstream target S6K1. Finally, we show that inhibition of hypothalamic ATF4 reverses hepatic insulin resistance induced by acute brain endoplasmic reticulum (ER) stress. Taken together, our study describes a novel central pathway regulating hepatic insulin sensitivity that is mediated by hypothalamic ATF4/mTOR/S6K1 signaling and the vagus nerve and demonstrates an important role for hypothalamic ATF4 in brain ER stress–induced hepatic insulin resistance. These results may lead to the identification of novel therapeutic targets for treating insulin resistance and associated metabolic diseases. PMID:23454693

  8. Transcription factor ATF4 directs basal and stress-induced gene expression in the unfolded protein response and cholesterol metabolism in the liver

    PubMed Central

    Fusakio, Michael E.; Willy, Jeffrey A.; Wang, Yongping; Mirek, Emily T.; Al Baghdadi, Rana J. T.; Adams, Christopher M.; Anthony, Tracy G.; Wek, Ronald C.

    2016-01-01

    Disturbances in protein folding and membrane compositions in the endoplasmic reticulum (ER) elicit the unfolded protein response (UPR). Each of three UPR sensory proteins—PERK (PEK/EIF2AK3), IRE1, and ATF6—is activated by ER stress. PERK phosphorylation of eIF2 represses global protein synthesis, lowering influx of nascent polypeptides into the stressed ER, coincident with preferential translation of ATF4 (CREB2). In cultured cells, ATF4 induces transcriptional expression of genes directed by the PERK arm of the UPR, including genes involved in amino acid metabolism, resistance to oxidative stress, and the proapoptotic transcription factor CHOP (GADD153/DDIT3). In this study, we characterize whole-body and tissue-specific ATF4-knockout mice and show in liver exposed to ER stress that ATF4 is not required for CHOP expression, but instead ATF6 is a primary inducer. RNA-Seq analysis indicates that ATF4 is responsible for a small portion of the PERK-dependent UPR genes and reveals a requirement for expression of ATF4 for expression of genes involved in oxidative stress response basally and cholesterol metabolism both basally and under stress. Consistent with this pattern of gene expression, loss of ATF4 resulted in enhanced oxidative damage, and increased free cholesterol in liver under stress accompanied by lowered cholesterol in sera. PMID:26960794

  9. Passive damping for space truss structures

    NASA Technical Reports Server (NTRS)

    Chen, Gun-Shing; Wada, Ben K.

    1988-01-01

    Theoretical and experimental studies of passive damping techniques in truss-type structures are presented, with emphasis on the use of viscoelastic damping in the parallel load path. The constraining member length is shown to be a convenient design variable for enhancing damping performance. Results are presented for integral damping members made of thin-wall aluminum tubes, concentric constraining members, and viscoelastic materials in a six-bay truss structure at low frequency and low dynamic strain conditions. Integral members with graphite/epoxy constraining members exhibited relatively low damping values due to the possible polymer interaction during the cocure stage.

  10. Forced oscillations with linear and nonlinear damping

    NASA Astrophysics Data System (ADS)

    Li, Aijun; Ma, Li; Keene, David; Klingel, Joshua; Payne, Marvin; Wang, Xiao-jun

    2016-01-01

    A general solution is derived for the differential equations of forced oscillatory motion with both linear damping ( ˜v ) and nonlinear damping ( ˜v2 ). Experiments with forced oscillators are performed using a flat metal plate with a drag force due to eddy currents and a flat piece of stiffened cardboard with a drag force due to air resistance serving as the linear and nonlinear damping, respectively. Resonance of forced oscillations for different damping forces and quality factors is demonstrated. The experimental measurements and theoretical calculations are in good agreement, and damping constants are determined.

  11. Clipped viscous damping with negative stiffness for semi-active cable damping

    NASA Astrophysics Data System (ADS)

    Weber, F.; Boston, C.

    2011-04-01

    This paper investigates numerically and experimentally clipped viscous damping with negative stiffness for semi-active cable damping. From simulations it is concluded that unclipped and clipped viscous damping with negative stiffness is equivalent to unclipped and clipped LQR. It is shown that optimized unclipped viscous damping with negative stiffness generates critical cable damping by an anti-node at the actuator position. The resulting curvature at the actuator position is larger than the curvature close to the anchors due to the disturbance forces which may lead to premature cable fatigue at the actuator position. Optimized clipped viscous damping with negative stiffness does not show this drawback, can be implemented using a semi-active damper and produces twice as much cable damping as optimal viscous damping. Close to the optimal tuning, it leads to approximately the same control force as optimal semi-active friction damping with negative stiffness, which explains the superior cable damping. The superior damping results from the negative stiffness that increases the damper motion. Clipped viscous damping with negative stiffness is validated on a strand cable with a magneto-rheological damper. The measured cable damping is twice that achieved by emulated viscous damping, which confirms the numerical results. A tuning rule for clipped viscous damping with negative stiffness of real cables with flexural rigidity is given.

  12. Reactivation of MASPIN in non-small cell lung carcinoma (NSCLC) cells by artificial transcription factors (ATFs)

    PubMed Central

    Beltran, Adriana S

    2011-01-01

    Tumor suppressor genes have antiproliferative and antimetastatic functions and thus, they negatively affect tumor progression. Reactivating specific tumor suppressor genes would offer an important therapeutic strategy to block tumor progression. Mammary serine protease inhibitor (MASPIN) is a tumor suppressor gene that is not mutated or rearranged in tumor cells, but is silenced during metastatic progression by transcriptional and epigenetic mechanisms. In this work, we have investigated the ability of artificial transcription factors (ATFs) to reactivate MASPIN expression and to reduce tumor growth and metastatic dissemination in non-small cell lung carcinoma (NSCLC) cell lines carrying a hypermethylated MASPIN promoter. We found that the ATFs linked to transactivator domains were able to demethylate the MASPIN promoter. Consistently, we observed that co-treatment of ATF-transduced cells with methyltransferase inhibitors enhanced MASPIN expression as well as induction of tumor cell apoptosis. In addition to tumor suppressive functions, restoration of endogenous MASPIN expression was accompanied by inhibition of metastatic dissemination in nude mice. ATF-mediated reactivation of MASPIN lead to changes in cell motility and to induction of E-CADHERIN. These data suggest that ATFs are able to reprogram aggressive lung tumor cells towards a more epithelial, differentiated phenotype and represent novel therapeutic agents for metastatic lung cancers. PMID:20948306

  13. The CR1 and CR3 domains of the adenovirus type 5 E1A proteins can independently mediate activation of ATF-2.

    PubMed Central

    Duyndam, M C; van Dam, H; van der Eb, A J; Zantema, A

    1996-01-01

    The adenovirus 12S E1A protein can stimulate the activity of the c-jun promoter through a conserved region 1 (CR1)-dependent mechanism. The effect is mediated by two AP-1/ATF-like elements, jun1 and jun2, that preferentially bind c-Jun-ATF-2 heterodimers. In this study, we show that the ATF-2 component of the c-Jun-ATF-2 heterodimer is the primary target for 12S E1A: 12S E1A can enhance the transactivating activity of the N terminus of ATF-2 when fused to a heterologous DNA-binding domain, whereas the transactivating activity of the c-Jun N terminus is not significantly affected. Activation of the ATF-2 N terminus by 12S E1A is dependent on CR1. In the context of the 13S E1A protein, CR1 and CR3 can both contribute to activation of ATF-2, and their relative contributions are dependent on the cell type. In contrast to activation of ATF-2 by stress-inducing agents, CR1-dependent activation of ATF-2 was found not to depend strictly on the presence of threonines 69 and 71 in the N terminus of ATF-2, which are targets for phosphorylation by stress-activated protein kinases (SAPKs). In agreement with this observation, we did not observe phosphorylation of threonines 69 and 71 or constitutively enhanced SAPK activity in E1A- plus E1B-transformed cell lines. These data suggest that CR1-dependent activation of ATF-2 by 12S E1A does not require phosphorylation of threonines 69 and 71 by SAPK. PMID:8709204

  14. Tests of the FONT3 Linear Collider Intra-Train Beam Feedback System at the ATF

    SciTech Connect

    Burrows, P.N.; Christian, G.; Clarke, C.; Hartin, A.; Dabiri Khah, H.; Molloy, S.; White, G.R.; Frisch, J.C.; Markiewicz, T.W.; McCormick, D.J.; Ross, M.C.; Smith, S.; Smith, T.J.; Kalinin, A.; Perry, C.; /Oxford Instruments

    2006-03-14

    We report preliminary results of beam tests of the FONT3 Linear Collider intra-train position feedback system prototype at the Accelerator Test Facility at KEK. The feedback system incorporates a novel beam position monitor (BPM) processor with a latency below 5 nanoseconds, and a kicker driver amplifier with similar low latency. The 56 nanosecond-long bunchtrain in the ATF extraction line was used to test the prototype BPM processor. The achieved latency will allow a demonstration of intra-train feedback on timescales relevant even for the CLIC Linear Collider design.

  15. Development and quality assessments of commercial heat production of ATF FeCrAl tubes

    SciTech Connect

    Yamamoto, Yukinori

    2015-09-01

    Development and quality assessment of the 2nd generation ATF FeCrAl tube production with commercial manufacturers were conducted. The manufacturing partners include Sophisticated Alloys, Inc. (SAI), Butler, PA for FeCrAl alloy casting via vacuum induction melting, Oak Ridge National Laboratory (ORNL) for extrusion process to prepare the master bars/tubes to be tube-drawn, and Rhenium Alloys, Inc. (RAI), North Ridgeville, OH, for tube-drawing process. The masters bars have also been provided to Los Alamos National Laboratory (LANL) who works with Century Tubes, Inc., (CTI), San Diego, CA, as parallel tube production effort under the current program.

  16. Resistive-wall instability at Fermilab recycler ring

    SciTech Connect

    Ng, King-Yuen B.; /Fermilab

    2004-11-01

    Sporadic transverse instabilities have been observed at the Fermilab Recycler Ring leading to increase in transverse emittances and beam loss. The driving source of these instabilities has been attributed to the resistive-wall impedance with space-charge playing an important role in suppressing Landau damping. Growth rates of the instabilities have been computed. Remaining problems are discussed.

  17. Compton backscattering of intracavity storage ring free-electron laser radiation

    SciTech Connect

    Dattoli, G.; Giannessi, L.; Torre, A.

    1995-12-31

    We discuss the{gamma}-ray production by Compton backscattering of intracavity storage ring Free-Electron Laser radiation. We use a semi-analytical model which provides the build up of the signal combined with the storage ring damping mechanism and derive simple relations yielding the connection between backscattered. Photons brightness and the intercavity laser equilibrium intensity.

  18. Fabrication Control Plan for ORNL RH-LOCA ATF Test Specimens to be Irradiated in the ATR

    SciTech Connect

    Kevin G. Field; Richard Howard; Michael Teague

    2014-06-01

    The purpose of this fabrication plan is (1) to summarize the design of a set of rodlets that will be fabricated and then irradiated in the Advanced Test Reactor (ATR) and (2) provide requirements for fabrication and acceptance criteria for inspections of the Light Water Reactor (LWR) – Accident Tolerant Fuels (ATF) rodlet components. The functional and operational (F&OR) requirements for the ATF program are identified in the ATF Test Plan. The scope of this document only covers fabrication and inspections of rodlet components detailed in drawings 604496 and 604497. It does not cover the assembly of these items to form a completed test irradiation assembly or the inspection of the final assembly, which will be included in a separate INL final test assembly specification/inspection document. The controls support the requirements that the test irradiations must be performed safely and that subsequent examinations must provide valid results.

  19. Targeting damage-associated molecular pattern molecules (DAMPs) and DAMP receptors in melanoma.

    PubMed

    Boone, Brian A; Lotze, Michael T

    2014-01-01

    Damage-associated molecular pattern molecules (DAMPs) are proteins released from cells under stress due to nutrient deprivation, hypoxia, trauma, or treatment with chemotherapy, among a variety of other causes. When released, DAMPs activate innate immunity, providing a pathway to a systemic inflammatory response in the absence of infection. By regulating inflammation in the tumor microenvironment, promoting angiogenesis, and increasing autophagy with evasion of apoptosis, DAMPs facilitate cancer growth. DAMPs and DAMP receptors have a key role in melanoma pathogenesis. Due to their crucial role in the development of melanoma and chemoresistance, DAMPs represent intriguing targets at a time when novel treatments are desperately needed. PMID:24258998

  20. Mitochondrial ATF2 translocation contributes to apoptosis induction and BRAF inhibitor resistance in melanoma through the interaction of Bim with VDAC1

    PubMed Central

    Deng, Chun; Guo, Chunbao

    2015-01-01

    Background The mitochondrial accumulation of ATF2 is involved in tumor suppressor activities via cytochrome c release in melanoma cells. However, the signaling pathways that connect mitochondrial ATF2 accumulation and cytochrome c release are not well documented. Methods Several melanoma cell lines, B16F10, K1735M2, A375 and A375-R1, were treated with paclitaxel and vemurafenib to test the function of mitochondrial ATF2 and its connection to Bim and voltage-dependent anion channel 1 (VDAC1). Immunoprecipitation analysis was performed to investigate the functional interaction between the involved proteins. VDAC1 oligomerization was evaluated using an EGS-based crosslinking assay. Results The expression and migration of ATF2 to the mitochondria accounted for paclitaxel stimuli and acquired resistance to BRAF inhibitors. Mitochondrial ATF2 facilitated Bim stabilization through the inhibition of its degradation by the proteasome, thereby promoting cytochrome c release and inducing apoptosis in B16F10 and A375 cells. Studies using B16F10 and A375 cells genetically modified for ATF2 indicated that mitochondrial ATF2 was able to dissociate Bim from the Mcl-1/Bim complex to trigger VDAC1 oligomerization. Immunoprecipitation analysis revealed that Bim interacts with VDAC1, and this interaction was remarkably enhanced during apoptosis. Conclusion These results reveal that mitochondrial ATF2 is associated with the induction of apoptosis and BRAF inhibitor resistance through Bim activation, which might suggest potential novel therapies for the targeted induction of apoptosis in melanoma therapy. PMID:26462148

  1. 27 CFR 555.34 - Replacement of stolen or lost ATF Form 5400.30 (Intrastate Purchase of Explosives Coupon (IPEC)).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... lost ATF Form 5400.30 (Intrastate Purchase of Explosives Coupon (IPEC)). 555.34 Section 555.34 Alcohol... or lost ATF Form 5400.30 (Intrastate Purchase of Explosives Coupon (IPEC)). When any Form 5400.30 is.... (Approved by the Office of Management and Budget under control number 1140-0077)...

  2. Performance of the FONT3 Fast Analogue Intra-Train Beam-Based Feedback System at ATF

    SciTech Connect

    Burrows, P.; Christian, G.B.; Hartin, A.F.; Dabiri Khah, H.; White, G.R.; Clarke, C.C.; Perry, C.; Kalinin, A.; McCormick, D.J.; Molloy, S.; Ross, M.C.; /SLAC

    2007-04-16

    We report results of beam tests of the FONT3 intra-train position feedback system prototype at the Accelerator Test Facility (ATF) at KEK. The feedback system incorporates a novel beam position monitor (BPM) processor with latency below 5 nanoseconds, and a kicker driver amplifier with similar low latency. The 56 nanosecond-long bunchtrain in the ATF extraction line was used to test the prototype feedback system. The achieved latency of 23ns provides a demonstration of intra-train feedback on very short timescales relevant even for the CLIC Linear Collider design.

  3. Introduction to DAMPE event reconstruction (On behalf of DAMPE collaboration)

    NASA Astrophysics Data System (ADS)

    Zang, Jingjing

    2016-07-01

    The Dark Matter Particle Explorer (DAMPE) is a high energy particle physics experiment satellite, launched on 17 Dec 2015. To measure basic attributes of cosmic ray particles, DAMPE is equipped with four sub-detectors, BGO calorimeter (BGO), plastic scintillator detector (PSD), silicon tungsten tracker (STK) and neutron detector (NUD). On orbit, the high energy particle data are acquired and recorded by well-designed Data Acquisition system. After that, a series of elaborate event reconstruction algorithms are implemented to determine the energy, direction and particle ID of each event. The energy reconstruction algorithm firstly treats the sum of the BGO crystal energy as the overall energy estimator and various corrections are performed to calculate energy leakage from side and back of the calorimeter. The track reconstruction starts with cluster finding in STK, then shower axis of BGO and barycentre of clusters are used to extract seed of tracks. These seeds will be projected on the next layer by Kalman Filter method which will finally give location and direction of particle tracks. Based on shower development in BGO and tracks reconstructed by STK, we also combine data from PSD and NUD and developed a series of algorithms to evaluate particle's charge and identification. In this talk, we will describe technical strategies of event reconstruction and provide their basic performance.

  4. ICAN/DAMP-integrated composite analyzer with damping analysis capabilities: User's manual

    NASA Technical Reports Server (NTRS)

    Saravanos, Dimitrious A.; Sanfeliz, Jose G.

    1992-01-01

    This manual describes the use of the computer code ICAN/DAMP (Integrated Composite Analyzer with Damping Analysis Capabilities) for the prediction of damping in polymer-matrix composites. The code is written in FORTRAN 77 and is a version of the ICAN (Integrated Composite ANalyzer) computer program. The code incorporates a new module for synthesizing the material damping from micromechanics to laminate level. Explicit micromechanics equations based on hysteretic damping are programmed relating the on-axis damping capacities to the fiber and matrix properties and fiber volume ratio. The damping capacities of unidirectional composites subjected to off-axis loading are synthesized from on-axis damping values. The hygrothermal effect on the damping performance of unidirectional composites caused by temperature and moisture variation is modeled along with the damping contributions from interfacial friction between broken fibers and matrix. The temperature rise is continuously vibrating composite plies and composite laminates is also estimated. The ICAN/DAMP user's manual provides descriptions of the damping analysis module's functions, structure, input requirements, output interpretation, and execution requirements. It only addresses the changes required to conduct the damping analysis and is used in conjunction with the 'Second Generation Integrated Composite Analyzer (ICAN) Computer Code' user's manual (NASA TP-3290).

  5. Viscous damping for base isolated structures

    SciTech Connect

    Lee, D.; Hussain, S.; Retamal, E.

    1995-12-01

    Seismic Base Isolation can use elastomeric pads, sliding plates or inverted pendulums. Each method can include an energy dissipation means, but only as some kind of hysteretic damping. Hysteretic damping has limitations in terms of energy absorption and may tend to excite higher modes in some cases. It`s possible to avoid these problems with viscous dampers. Viscous damping adds energy dissipation through loads that are 900 out of phase with bending and shear loads so even with damping levels as high as 40% of critical adverse side effects tend to be minimal. This paper presents basic theory of viscous damping, and also describes a sample project. Viscous dampers being built for the new San Bernardino Medical Center reduce both deflections and loads by 50% compared with high damping elastomer base isolation bearings by themselves.

  6. Cryptocephal, the Drosophila melanogaster ATF4, Is a Specific Coactivator for Ecdysone Receptor Isoform B2

    PubMed Central

    Gauthier, Sebastien A.; VanHaaften, Eric; Cherbas, Lucy; Cherbas, Peter; Hewes, Randall S.

    2012-01-01

    The ecdysone receptor is a heterodimer of two nuclear receptors, the Ecdysone receptor (EcR) and Ultraspiracle (USP). In Drosophila melanogaster, three EcR isoforms share common DNA and ligand-binding domains, but these proteins differ in their most N-terminal regions and, consequently, in the activation domains (AF1s) contained therein. The transcriptional coactivators for these domains, which impart unique transcriptional regulatory properties to the EcR isoforms, are unknown. Activating transcription factor 4 (ATF4) is a basic-leucine zipper transcription factor that plays a central role in the stress response of mammals. Here we show that Cryptocephal (CRC), the Drosophila homolog of ATF4, is an ecdysone receptor coactivator that is specific for isoform B2. CRC interacts with EcR-B2 to promote ecdysone-dependent expression of ecdysis-triggering hormone (ETH), an essential regulator of insect molting behavior. We propose that this interaction explains some of the differences in transcriptional properties that are displayed by the EcR isoforms, and similar interactions may underlie the differential activities of other nuclear receptors with distinct AF1-coactivators. PMID:22912598

  7. Loading, absorption, and Fokker-Planck calculations for upcoming ICRF experiments on ATF

    SciTech Connect

    Shepard, T.D.; Carter, M.D.; Goulding, R.H.; Kwon, M.

    1989-01-01

    ICRF experiments on ATF at the 100-kW level are planned for the current 1989 operating period. These plans include the 2..omega../sub cH/ regime at f/sub RF/ = 28.88 MHz, D(H) at 14.44 MHz, and /sup 4/He(/sup 3/He) and D(/sup 3/He) at 9.63 MHz. ECH target plasmas have n/sub eO/ /approx lt/ 0.15 /times/ 10/sup 20/ m/sup /minus/3/ and B = 0.95 T. The density and temperature profiles obtained are broader than those from 1988, owing to recent field error corrections. The values used for target-plasma parameters in the calculations were taken from initial 1989 ATF data. Loading and absorption calculations have been performed using the 3D RF heating code ORION with a helically symmetric equilibrium, and Fokker-Planck calculations were performed using the steady-state code RFTRANS with two velocity dimensions and one spatial dimension. 6 refs., 3 figs.

  8. Experience with a high-brightness storage ring: the NSLS 750 MeV vuv ring

    SciTech Connect

    Galayda, J.

    1984-01-01

    The NSLS vuv ring is the first implementation of the proposals of R. Chasman and G.K. Green for a synchrotron radiation source with enhanced brightness: its lattice is a series of achromatic bends with two zero-gradient dipoles each, giving small damped emittance; and these bends are connected by straight sections with zero dispersion to accommodate wigglers and undulators without degrading the radiation damping properties of the ring. The virtues of the Chasman-Green lattice, its small betatron and synchrotron emittances, may be understood with some generality; e.g. the electron ..gamma..m/sub 0/c/sup 2/ energy and the number of achromatic bends M sets a lower limit on the betatron emittance of e/sub x/ > 7.7 x 10/sup -13/ ..gamma../sup 2//M meter-radians. There is strong interest in extrapolation of this type of lattice to 6 GeV and to 32 achromatic bends. The subject of this report is the progress toward achieving performance in the vuv ring limited by the radiation damping parameters optimized in its design. 14 refs., 4 figs., 1 tab.

  9. CHARACTERIZATION OF DAMPING IN BOLTED LAP JOINTS

    SciTech Connect

    C. MALONEY; D. PEAIRS; ET AL

    2000-08-01

    The dynamic response of a jointed beam was measured in laboratory experiments. The data were analyzed and the system was mathematically modeled to establish plausible representations of joint damping behavior. Damping is examined in an approximate, local linear framework using log decrement and half power bandwidth approaches. in addition, damping is modeled in a nonlinear framework using a hybrid surface irregularities model that employs a bristles-construct. Experimental and analytical results are presented.

  10. Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa

    PubMed Central

    Bhootada, Yogesh; Kotla, Pravallika; Zolotukhin, Sergei; Gorbatyuk, Oleg; Bebok, Zsuzsanna; Athar, Mohammad; Gorbatyuk, Marina

    2016-01-01

    T17M rhodopsin expression in rod photoreceptors leads to severe retinal degeneration and is associated with the activation of ER stress related Unfolded Protein Response (UPR) signaling. Here, we show a novel role of a UPR transcription factor, ATF4, in photoreceptor cellular pathology. We demonstrated a pro-death role for ATF4 overexpression during autosomal dominant retinitis pigmentosa (ADRP). Based on our results in ATF4 knockout mice and adeno-associated viral (AAV) delivery of ATF4 to the retina, we validated a novel therapeutic approach targeting ATF4 over the course of retinal degeneration. In T17M rhodopsin retinas, we observed ATF4 overexpression concomitantly with reduction of p62 and elevation of p53 levels. These molecular alterations, together with increased CHOP and caspase-3/7 activity, possibly contributed to the mechanism of photoreceptor cell loss. Conversely, ATF4 knockdown retarded retinal degeneration in 1-month-old T17M Rhodopsin mice and promoted photoreceptor survival, as measured by scotopic and photopic ERGs and photoreceptor nuclei row counts. Similarly, ATF4 knockdown also markedly delayed retinal degeneration in 3-month-old ADRP animals. This delay was accompanied by a dramatic decrease in UPR signaling, the launching of anti-oxidant defense, initiation of autophagy, and improvement of rhodopsin biosynthesis which together perhaps combat the cellular stress associated with T17M rhodopsin. Our data indicate that augmented ATF4 signals during retinal degeneration plays a cytotoxic role by triggering photoreceptor cell death. Future ADRP therapy regulating ATF4 expression can be developed to treat retinal degenerative disorders associated with activated UPR. PMID:27144303

  11. CARMA1- and MyD88-dependent activation of Jun/ATF-type AP-1 complexes is a hallmark of ABC diffuse large B-cell lymphomas

    PubMed Central

    Juilland, Mélanie; Gonzalez, Montserrat; Erdmann, Tabea; Banz, Yara; Jevnikar, Zala; Hailfinger, Stephan; Tzankov, Alexandar; Grau, Michael; Lenz, Georg; Novak, Urban

    2016-01-01

    A hallmark of the diffuse large B-cell lymphoma (DLBCL) of the activated B-cell (ABC) type, a molecular subtype characterized by adverse outcome, is constitutive activation of the transcription factor nuclear factor–κB (NF-κB), which controls expression of genes promoting cellular survival and proliferation. Much less, however, is known about the role of the transcription factor activator protein-1 (AP-1) in ABC DLBCL. Here, we show that AP-1, like NF-κB, was controlled by constitutive activation of the B-cell receptor signaling component caspase recruitment domain-containing membrane-associated guanylate kinase 1 (CARMA1) and/or the Toll-like receptor signaling component myeloid differentiation primary response gene 88 (MyD88) in ABC DLBCL cell lines. In contrast to germinal center (GC) B-cell (GCB) DLBCL, ABC DLBCL cell lines expressed high levels of the AP-1 family members c-Jun, JunB, and JunD, which formed heterodimeric complexes with the AP-1 family members activating transcription factor (ATF) 2, ATF3, and ATF7. Inhibition of these complexes by a dominant-negative approach led to impaired growth of a majority of ABC DLBCL cell lines. Individual silencing of c-Jun, ATF2, or ATF3 decreased cellular survival and revealed c-Jun/ATF2-dependent control of ATF3 expression. As a consequence, ATF3 expression was much higher in ABC vs GCB DLBCL cell lines. Samples derived from DLBCL patients showed a clear trend toward high and nuclear ATF3 expression in nodal DLBCL of the non-GC or ABC subtype. These findings identify the activation of AP-1 complexes of the Jun/ATF-type as an important element controlling the growth of ABC DLBCL. PMID:26747248

  12. Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa.

    PubMed

    Bhootada, Yogesh; Kotla, Pravallika; Zolotukhin, Sergei; Gorbatyuk, Oleg; Bebok, Zsuzsanna; Athar, Mohammad; Gorbatyuk, Marina

    2016-01-01

    T17M rhodopsin expression in rod photoreceptors leads to severe retinal degeneration and is associated with the activation of ER stress related Unfolded Protein Response (UPR) signaling. Here, we show a novel role of a UPR transcription factor, ATF4, in photoreceptor cellular pathology. We demonstrated a pro-death role for ATF4 overexpression during autosomal dominant retinitis pigmentosa (ADRP). Based on our results in ATF4 knockout mice and adeno-associated viral (AAV) delivery of ATF4 to the retina, we validated a novel therapeutic approach targeting ATF4 over the course of retinal degeneration. In T17M rhodopsin retinas, we observed ATF4 overexpression concomitantly with reduction of p62 and elevation of p53 levels. These molecular alterations, together with increased CHOP and caspase-3/7 activity, possibly contributed to the mechanism of photoreceptor cell loss. Conversely, ATF4 knockdown retarded retinal degeneration in 1-month-old T17M Rhodopsin mice and promoted photoreceptor survival, as measured by scotopic and photopic ERGs and photoreceptor nuclei row counts. Similarly, ATF4 knockdown also markedly delayed retinal degeneration in 3-month-old ADRP animals. This delay was accompanied by a dramatic decrease in UPR signaling, the launching of anti-oxidant defense, initiation of autophagy, and improvement of rhodopsin biosynthesis which together perhaps combat the cellular stress associated with T17M rhodopsin. Our data indicate that augmented ATF4 signals during retinal degeneration plays a cytotoxic role by triggering photoreceptor cell death. Future ADRP therapy regulating ATF4 expression can be developed to treat retinal degenerative disorders associated with activated UPR. PMID:27144303

  13. Understanding the damped SHM without ODEs

    NASA Astrophysics Data System (ADS)

    Ng, Chiu-king

    2016-03-01

    Instead of solving ordinary differential equations (ODEs), the damped simple harmonic motion (SHM) is surveyed qualitatively from basic mechanics and quantitatively by the instrumentality of a graph of velocity against displacement. In this way, the condition b≥slant \\sqrt{4mk}~ for the occurrence of the non-oscillating critical damping and heavy-damping is derived. Besides, we prove in the under-damping, the oscillation is isochronous and the diminishing amplitude satisfies a rule of ‘constant ratio’. All are done on a non-ODE basis.

  14. Damping constant estimation in magnetoresistive readers

    SciTech Connect

    Stankiewicz, Andrzej Hernandez, Stephanie

    2015-05-07

    The damping constant is a key design parameter in magnetic reader design. Its value can be derived from bulk or sheet film ferromagnetic resonance (FMR) line width. However, dynamics of nanodevices is usually defined by presence of non-uniform modes. It triggers new damping mechanisms and produces stronger damping than expected from traditional FMR. This work proposes a device-level technique for damping evaluation, based on time-domain analysis of thermally excited stochastic oscillations. The signal is collected using a high bandwidth oscilloscope, by direct probing of a biased reader. Recorded waveforms may contain different noise signals, but free layer FMR is usually a dominating one. The autocorrelation function is a reflection of the damped oscillation curve, averaging out stochastic contributions. The damped oscillator formula is fitted to autocorrelation data, producing resonance frequency and damping constant values. Restricting lag range allows for mitigation of the impact of other phenomena (e.g., reader instability) on the damping constant. For a micromagnetically modeled reader, the technique proves to be much more accurate than the stochastic FMR line width approach. Application to actual reader waveforms yields a damping constant of ∼0.03.

  15. Viscous damped space structure for reduced jitter

    NASA Technical Reports Server (NTRS)

    Wilson, James F.; Davis, L. Porter

    1987-01-01

    A technique to provide modal vibration damping in high performance space structures was developed which uses less than one once of incompressible fluid. Up to 50 percent damping can be achieved which can reduce the settling times of the lowest structural mode by as much as 50 to 1. This concept allows the designers to reduce the weight of the structure while improving its dynamic performance. Damping by this technique is purely viscous and has been shown by test to be linear over 5 orders of input magnitude. Amplitudes as low as 0.2 microinch were demonstrated. Damping in the system is independent of stiffness and relatively insensitive to temperature.

  16. Phenomenology of chiral damping in noncentrosymmetric magnets

    NASA Astrophysics Data System (ADS)

    Akosa, Collins Ashu; Miron, Ioan Mihai; Gaudin, Gilles; Manchon, Aurélien

    2016-06-01

    A phenomenology of magnetic chiral damping is proposed in the context of magnetic materials lacking inversion symmetry. We show that the magnetic damping tensor acquires a component linear in magnetization gradient in the form of Lifshitz invariants. We propose different microscopic mechanisms that can produce such a damping in ferromagnetic metals, among which local spin pumping in the presence of an anomalous Hall effect and an effective "s-d" Dzyaloshinskii-Moriya antisymmetric exchange. The implication of this chiral damping in terms of domain-wall motion is investigated in the flow and creep regimes.

  17. Damping constant estimation in magnetoresistive readers

    NASA Astrophysics Data System (ADS)

    Stankiewicz, Andrzej; Hernandez, Stephanie

    2015-05-01

    The damping constant is a key design parameter in magnetic reader design. Its value can be derived from bulk or sheet film ferromagnetic resonance (FMR) line width. However, dynamics of nanodevices is usually defined by presence of non-uniform modes. It triggers new damping mechanisms and produces stronger damping than expected from traditional FMR. This work proposes a device-level technique for damping evaluation, based on time-domain analysis of thermally excited stochastic oscillations. The signal is collected using a high bandwidth oscilloscope, by direct probing of a biased reader. Recorded waveforms may contain different noise signals, but free layer FMR is usually a dominating one. The autocorrelation function is a reflection of the damped oscillation curve, averaging out stochastic contributions. The damped oscillator formula is fitted to autocorrelation data, producing resonance frequency and damping constant values. Restricting lag range allows for mitigation of the impact of other phenomena (e.g., reader instability) on the damping constant. For a micromagnetically modeled reader, the technique proves to be much more accurate than the stochastic FMR line width approach. Application to actual reader waveforms yields a damping constant of ˜0.03.

  18. Relaxation damping in oscillating contacts

    PubMed Central

    Popov, M.; Popov, V.L.; Pohrt, R.

    2015-01-01

    If a contact of two purely elastic bodies with no sliding (infinite coefficient of friction) is subjected to superimposed oscillations in the normal and tangential directions, then a specific damping appears, that is not dependent on friction or dissipation in the material. We call this effect “relaxation damping”. The rate of energy dissipation due to relaxation damping is calculated in a closed analytic form for arbitrary axially-symmetric contacts. In the case of equal frequency of normal and tangential oscillations, the dissipated energy per cycle is proportional to the square of the amplitude of tangential oscillation and to the absolute value of the amplitude of normal oscillation, and is dependent on the phase shift between both oscillations. In the case of low frequency tangential oscillations with superimposed high frequency normal oscillations, the dissipation is proportional to the ratio of the frequencies. Generalization of the results for macroscopically planar, randomly rough surfaces as well as for the case of finite friction is discussed. PMID:26549011

  19. Relaxation damping in oscillating contacts

    NASA Astrophysics Data System (ADS)

    Popov, M.; Popov, V. L.; Pohrt, R.

    2015-11-01

    If a contact of two purely elastic bodies with no sliding (infinite coefficient of friction) is subjected to superimposed oscillations in the normal and tangential directions, then a specific damping appears, that is not dependent on friction or dissipation in the material. We call this effect “relaxation damping”. The rate of energy dissipation due to relaxation damping is calculated in a closed analytic form for arbitrary axially-symmetric contacts. In the case of equal frequency of normal and tangential oscillations, the dissipated energy per cycle is proportional to the square of the amplitude of tangential oscillation and to the absolute value of the amplitude of normal oscillation, and is dependent on the phase shift between both oscillations. In the case of low frequency tangential oscillations with superimposed high frequency normal oscillations, the dissipation is proportional to the ratio of the frequencies. Generalization of the results for macroscopically planar, randomly rough surfaces as well as for the case of finite friction is discussed.

  20. Superconductive material and magnetic field for damping and levitation support and damping of cryogenic instruments

    NASA Technical Reports Server (NTRS)

    Dolgin, Benjamin P. (Inventor)

    1994-01-01

    A superconductive load bearing support without a mechanical contact and vibration damping for cryogenic instruments in space is presented. The levitation support and vibration damping is accomplished by the use of superconducting magnets and the 'Meissner' effect. The assembly allows for transfer of vibration energy away from the cryogenic instrument which then can be damped by the use of either an electronic circuit or conventional vibration damping mean.

  1. Validation of High-Resolution CFD Method for Slosh Damping Extraction of Baffled Cryogenic Propellant Tanks

    NASA Technical Reports Server (NTRS)

    Yang, H. Q.; West, Jeff

    2016-01-01

    Propellant slosh is a potential source of disturbance critical to the stability of space vehicles. The slosh dynamics are typically represented by a mechanical model of a spring-mass-damper. This mechanical model is then included in the equation of motion of the entire vehicle for Guidance, Navigation and Control analysis. A Volume-Of-Fluid (VOF) based Computational Fluid Dynamics (CFD) program developed at MSFC was applied to extract slosh damping in the baffled tank from the first principle. First the experimental data using water with sub-scale smooth wall tank were used as the baseline validation. It is demonstrated that CFD can indeed accurately predict low damping values from the smooth wall at different fill levels. The damping due to a ring baffles at different depths from the free surface was then simulated, and fairly good agreement with experimental measurement was observed. Comparison with an empirical correlation of Miles equation is also made.

  2. Controllable damping of high-Q violin modes in fused silica suspension fibers

    NASA Astrophysics Data System (ADS)

    Dmitriev, A. V.; Mescheriakov, S. D.; Tokmakov, K. V.; Mitrofanov, V. P.

    2010-01-01

    Fused silica fiber suspension of the test masses will be used in the interferometric gravitational wave detectors of the next generation. This allows a significant reduction of losses in the suspension and thermal noise associated with the suspension. Unfortunately, unwanted violin modes may be accidentally excited in the suspension fibers. The Q-factor of the violin modes also exceeds 108. They have a ring-down time that is too long and may complicate the stable control of the interferometer. Results of the investigation of a violin mode active damping system are described. An original sensor and actuator were especially developed to realize the effective coupling of a thin, optically transparent, non-conducting fused silica fiber with an electric circuit. The damping system allowed the changing of the violin mode's damping rate over a wide range.

  3. Tuned vibration absorbers with nonlinear viscous damping for damped structures under random load

    NASA Astrophysics Data System (ADS)

    Shum, K. M.

    2015-06-01

    The classical problem for the application of a tuned vibration absorber is to minimize the response of a structural system, such as displacement, velocity, acceleration or to maximize the energy dissipated by tuned vibration absorber. The development of explicit optimal absorber parameters is challenging for a damped structural system since the fixed points no longer exist in the frequency response curve. This paper aims at deriving a set of simple design formula of tuned vibration absorber with nonlinear viscous damping based on the frequency tuning for harmonic load for a damped structural system under white noise excitation. The vibration absorbers being considered include tuned mass damper (TMD) and liquid column vibration absorber (LCVA). Simple approximate expression for the standard deviation velocity response of tuned vibration absorber for damped primary structure is also derived in this study to facilitate the estimation of the damping coefficient of TMD with nonlinear viscous damping and the head loss coefficient of LCVA. The derived results indicate that the higher the structural inherent damping the smaller the supplementary damping provided by a tuned vibration absorber. Furthermore, the optimal damping of tuned vibration absorber is shown to be independent of structural damping when it is tuned using the frequency tuning for harmonic load. Finally, the derived closed-form expressions are demonstrated to be capable of predicting the optimal parameters of tuned vibration absorbers with sufficient accuracy for preliminary design of tuned vibration absorbers with nonlinear viscous damping for a damped primary structure.

  4. ATF4 (activating transcription factor 4) from grass carp (Ctenopharyngodon idella) modulates the transcription initiation of GRP78 and GRP94 in CIK cells.

    PubMed

    Fan, Qidi; Mao, Huiling; Wu, Chuxin; Liu, Yong; Hu, Yousheng; Zhong, Bin; Mi, Yichuan; Hu, Chengyu

    2014-05-01

    GRP78 and GRP94, belong to GRP (glucose-regulated protein) family of endoplasmatic reticulum (ER) chaperone superfamily, are essential for cell survival under ER stress. ATF4 is a protective protein which regulates the adaptation of cells to ER stress by modulating the transcription of UPR (Unfolded Protein Response) target genes, including GRP78 and GRP94. To understand the molecular mechanism of ATF4 modulates the transcription initiation of CiGRP78 and CiGRP94, we cloned ATF4 ORF cDNA sequences (CiATF4) by homologous cloning techniques. The expression trend of CiATF4 was similar to CiGRP78 and CiGRP94 did under 37 °C thermal stress, namely, the expression of CiATF4 was up-regulated twice at 2 h post-thermal stress and at 18 h post recovery from thermal stress. In this paper, CiATF4 was expressed in BL21 Escherichia coli, and the expressed protein was purified by affinity chromatography with the Ni-NTA His-Bind Resin. On the basis of the cloned CiGRP78 and CiGRP94 cDNA in our laboratory previously, we cloned their promoter sequences by genomic walking approach. In vitro, gel mobility shift assays revealed that CiATF4 could bind to CiGRP78 and CiGRP94 promoter with high affinity. Subsequently, the recombinant plasmid of pGL3-CiGRPs and pcDNA3.1-CiATF4 were constructed and transiently co-transfected into Ctenopharyngodon idella kidney (CIK) cells. The impact of CiATF4 on CiGRP promoter sequences were measured by luciferase assays. These results demonstrated that CiATF4 could activate the transcription of CiGRP78 and CiGRP94. What's more, for better understanding the molecular mechanism of CiATF4 modulate the transcription initiation of CiGRP, three mutant fragments of CiGRP78 promoter recombinant plasmids (called CARE-mut/LUC, CRE1-mut/LUC and CRE2-mut/LUC) were constructed and transiently co-transfected with CiATF4 into CIK cells. The results indicated that CRE or CARE elements were the regulatory element for transcription initiation of CiGRP78. Between them, CRE

  5. Prediction of Liquid Slosh Damping Using a High Resolution CFD Tool

    NASA Technical Reports Server (NTRS)

    Yang, H. Q.; Purandare, Ravi; Peugeot, John; West, Jeff

    2012-01-01

    Propellant slosh is a potential source of disturbance critical to the stability of space vehicles. The slosh dynamics are typically represented by a mechanical model of a spring mass damper. This mechanical model is then included in the equation of motion of the entire vehicle for Guidance, Navigation and Control analysis. Our previous effort has demonstrated the soundness of a CFD approach in modeling the detailed fluid dynamics of tank slosh and the excellent accuracy in extracting mechanical properties (slosh natural frequency, slosh mass, and slosh mass center coordinates). For a practical partially-filled smooth wall propellant tank with a diameter of 1 meter, the damping ratio is as low as 0.0005 (or 0.05%). To accurately predict this very low damping value is a challenge for any CFD tool, as one must resolve a thin boundary layer near the wall and must minimize numerical damping. This work extends our previous effort to extract this challenging parameter from first principles: slosh damping for smooth wall and for ring baffle. First the experimental data correlated into the industry standard for smooth wall were used as the baseline validation. It is demonstrated that with proper grid resolution, CFD can indeed accurately predict low damping values from smooth walls for different tank sizes. The damping due to ring baffles at different depths from the free surface and for different sizes of tank was then simulated, and fairly good agreement with experimental correlation was observed. The study demonstrates that CFD technology can be applied to the design of future propellant tanks with complex configurations and with smooth walls or multiple baffles, where previous experimental data is not available.

  6. CONTRIBUTION OF INSPIRATORY FLOW TO ACTIVATION OF EGFR, RAS, MAPK, ATF-2 AND C-JUN DURING LUNG STRETCH

    EPA Science Inventory

    Contribution of Inspiratory Flow to Activation of EGFR, Ras, MAPK, ATF-2 and c-Jun during Lung Stretch

    R. Silbajoris 1, Z. Li 2, J. M. Samet 1 and Y. C. Huang 1. 1 NHEERL, ORD, US EPA, RTP, NC and 2 CEMALB, UNC-CH, Chapel Hill, NC .

    Mechanical ventilation with larg...

  7. Novel mechanism of enhancing IRE1α-XBP1 signalling via the PERK-ATF4 pathway

    PubMed Central

    Tsuru, Akio; Imai, Yasutaka; Saito, Michiko; Kohno, Kenji

    2016-01-01

    Mammalian inositol-requiring enzyme 1α (IRE1α) is the most conserved of all endoplasmic reticulum (ER) stress sensors, which includes activating transcription factor (ATF) 6 and double-stranded RNA-dependent protein kinase (PKR)-like ER kinase (PERK). IRE1α has been known to splice X-box binding protein 1 (XBP1) mRNA, which is induced by ATF6 under ER stress. This spliced XBP1 mRNA is translated into the active transcription factor that promotes the expression of specific genes to alleviate ER stress. Herein, we report that in addition to the induction of XBP1 expression by ATF6, IRE1α expression is induced by ATF4, which is downstream of PERK, under ER stress. Increased IRE1α expression results in a higher splicing ratio of XBP1 mRNA. This effect was not transient and affected not only the intensity but also the duration of the activated state of this pathway. These multiple regulatory mechanisms may modulate the response to various levels or types of ER stress. PMID:27052593

  8. Finding ATF4/p75NTR/IL-8 Signal Pathway in Endothelial–Mesenchymal Transition by Safrole Oxide

    PubMed Central

    Zhao, Wenbo; Yue, Hongwei; Su, Le; Zhang, ShangLi; Zhao, Jing

    2014-01-01

    Targeting the endothelial-to-mesenchymal transition (EndoMT) may be a novel therapeutic strategy for cancer and various diseases induced by fibrosis. We aimed to identify a small chemical molecule as an inducer of EndoMT and find a new signal pathway by using the inducer. Safrole oxide (SFO), 50 µg/ml, could most effectively induce EndoMT within 12 h. To understand the underlying molecular mechanism, we performed microarray, quantitative real-time PCR and western blot analysis to find key factors involved in SFO-induced EndoMT and demonstrated the involvement of the factors by RNAi. The expression of activating transcription factor 4 (ATF4), p75 neurotrophin receptor (p75NTR), and interleukin 8 (IL-8) was greatly increased in SFO-induced EndoMT. Knockdown of ATF4 inhibited the SFO-induced EndoMT completely, and knockdown of p75NTR or IL-8 partially inhibited the EndoMT, which suggests that all three factors were involved in the process. Furthermore, knockdown of p75NTR inhibited the SFO-increased IL-8 expression and secretion, and knockdown of ATF4 inhibited SFO-increased p75NTR level significantly. The ATF4/p75NTR/IL-8 signal pathway may have an important role in EndoMT induced by SFO. Our findings support potential novel targets for the therapeutics of cancer and fibrosis disease. PMID:24905361

  9. Expression of ATF4 and RUNX2 in periodontal tissue of pressure side during orthodontic tooth movement in rat

    PubMed Central

    Han, Jinyou; Xu, Xiaodong; Zhang, Bin; Chen, Baoxing; Hang, Wangyan

    2015-01-01

    Objective: This study aims to explore the expression levels of ATF4 and RUNX and their interactions in periodontal tissue of the pressure side during orthodontic tooth movement. Methods: A total of 72 SPF level male Sprague Dawley rats were used in this study, they were divided into 9 groups randomly and 8 rats in each group. The expression changes of ATF4 and RUNX2 in periodontal tissue of pressure side at different straining time point were detected with RT-PCR and Western blotting methods. The morphological changes of cells in the tissue samples were observed by HE staining. The data were analyzed with SPSS 19.0 software. Results: The expression levels of ATF4 and RUNX2 increased during orthodontic tooth movement and were related with the movement time. They reached highest after straining for 24 h and began to decrease after straining for 12 d. Conclusions: The expression levels of ATF4 and RUNX2 in periodontal tissue can increase transiently induced by stress, which play a role in the process of osteogenesis and reconstruction of periodontal tissue during orthodontic tooth movement. PMID:25785078

  10. Coffee Polyphenols Change the Expression of STAT5B and ATF-2 Modifying Cyclin D1 Levels in Cancer Cells

    PubMed Central

    Oleaga, Carlota; Ciudad, Carlos J.; Noé, Véronique; Izquierdo-Pulido, Maria

    2012-01-01

    Background. Epidemiological studies suggest that coffee consumption reduces the risk of cancer, but the molecular mechanisms of its chemopreventive effects remain unknown. Objective. To identify differentially expressed genes upon incubation of HT29 colon cancer cells with instant caffeinated coffee (ICC) or caffeic acid (CA) using whole-genome microarrays. Results. ICC incubation of HT29 cells caused the overexpression of 57 genes and the underexpression of 161, while CA incubation induced the overexpression of 12 genes and the underexpression of 32. Using Venn-Diagrams, we built a list of five overexpressed genes and twelve underexpressed genes in common between the two experimental conditions. This list was used to generate a biological association network in which STAT5B and ATF-2 appeared as highly interconnected nodes. STAT5B overexpression was confirmed at the mRNA and protein levels. For ATF-2, the changes in mRNA levels were confirmed for both ICC and CA, whereas the decrease in protein levels was only observed in CA-treated cells. The levels of cyclin D1, a target gene for both STAT5B and ATF-2, were downregulated by CA in colon cancer cells and by ICC and CA in breast cancer cells. Conclusions. Coffee polyphenols are able to affect cyclin D1 expression in cancer cells through the modulation of STAT5B and ATF-2. PMID:22919439

  11. 28 CFR 16.106 - Exemption of the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF)-Limited Access.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Exemption of the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF)-Limited Access. 16.106 Section 16.106 Judicial Administration DEPARTMENT OF JUSTICE PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Exemption of Records Systems Under the Privacy Act § 16.106 Exemption of...

  12. Asymmetric dipolar ring

    DOEpatents

    Prosandeev, Sergey A.; Ponomareva, Inna V.; Kornev, Igor A.; Bellaiche, Laurent M.

    2010-11-16

    A device having a dipolar ring surrounding an interior region that is disposed asymmetrically on the ring. The dipolar ring generates a toroidal moment switchable between at least two stable states by a homogeneous field applied to the dipolar ring in the plane of the ring. The ring may be made of ferroelectric or magnetic material. In the former case, the homogeneous field is an electric field and in the latter case, the homogeneous field is a magnetic field.

  13. MiR-214 regulates the function of osteoblast under simulated microgravity by targeting ATF4

    NASA Astrophysics Data System (ADS)

    Li, Yingxian; Wang, Xiaogang; Li, Qi; Lv, Ke; Wan, Yumin; Li, Yinghui; Bai, Yanqiang

    Background: MicroRNAs (miRNAs) are small fragments of single-stranded RNA containing 18-24 nucleotides, and are generated from endogenous transcripts. MicroRNAs function in post-transcriptional gene silencing by targeting the 3'-untranslated region (UTR) of mRNAs, resulting in translational repression. Growing evidence shows that microRNAs (miRNAs) regu-late various developmental and homeostatic events in vertebrates and invertebrates. Osteoblast differentiation is a key step in proper skeletal development and acquisition of bone mass; How-ever, the physiological role of non-coding small RNAs, especially miRNAs, in osteoblast dif-ferentiation remains elusive. Methods: To study the potential involvement of miRNAs in osteoblast differentiation under stimulated microgravity, we analyzed the expression of 20 bone relative miRNAs using real time PCR platform to find particularly miRNAs whose expression is altered during osteoblast differentiation. TargetScan, miRBase and Miranda were used to predict the target gene of candidate miRNA. To investigate whether ATF4 can be directly targeted by miR-214, we engineered luciferase reporters that have either the wild-type 3'UTRs of these genes, or the mutant UTRs with a 6 base pair (bp) deletion in the target sites. Lastly, to address the in vivo role of miR-214 in bone formation, tail suspension mice model was used to simulate the change of osteoblast function and bone loss. Results: Recent studies have sug-gested that miRNAs might play a role in osteoblast differentiation and bone formation. Here, we identify miR-214 in MC3T3-E1 cells, which is a primary mouse osteoblasts cell line, to promote osteoblast differentiation by repressing Activating Transcription Factor4 (ATF4) ex-pression at the posttranscriptional level. What is more, miR-214 was found to be transcribed in C2C12 cells during bone morphogenetic protein 2-induced (BMP2-induced) osteogenesis, and overexpression of miR-214 attenuated BMP2-induced osteoblastogenesis

  14. HOME DAMPNESS AND RESPIRATORY MORBIDITY IN CHILDREN

    EPA Science Inventory

    This study examined the relationship between measures of home dampness and respiratory illness and symptoms in a cohort of 4,625 eight- to 12-year old children in six U.S. cities. ome dampness was characterized from questionnaire reports of mold or mildew damage inside the home, ...

  15. Passive damping in EDS maglev systems.

    SciTech Connect

    Rote, D. M.

    2002-05-03

    There continues to be strong interest in the subjects of damping and drag forces associated with electrodynamic suspension (EDS) systems. While electromagnetic drag forces resist the forward motion of a vehicle and therefore consume energy, damping forces control, at least in part, the response of the vehicle to disturbances. Ideally, one would like to reduce the drag forces as much as possible while retaining adequate damping forces to insure dynamic stability and satisfactory ride quality. These two goals turn out to be difficult to achieve in practice. It is well known that maglev systems tend to be intrinsically under damped. Consequently it is often necessary in a practical system design to enhance the damping passively or actively. For reasons of cost and simplicity, it is desirable to rely as much as possible on passive damping mechanisms. In this paper, rough estimates are made of the passive damping and drag forces caused by various mechanisms in EDS systems. No attention will be given to active control systems or secondary suspension systems which are obvious ways to augment passive damping mechanisms if the latter prove to be inadequate.

  16. Understanding the Damped SHM without ODEs

    ERIC Educational Resources Information Center

    Ng, Chiu-king

    2016-01-01

    Instead of solving ordinary differential equations (ODEs), the damped simple harmonic motion (SHM) is surveyed qualitatively from basic mechanics and quantitatively by the instrumentality of a graph of velocity against displacement. In this way, the condition b ? [square root]4mk for the occurrence of the non-oscillating critical damping and…

  17. Status report of RMS active damping augmentation

    NASA Technical Reports Server (NTRS)

    Gilbert, Mike; Demeo, Martha E.

    1993-01-01

    A status report of Remote Manipulator System (RMS) active damping augmentation is presented. Topics covered include: active damping augmentation; benefits of RMS ADA; simulated payload definition; sensor and actuator definition; ADA control law design; Shuttle Engineering Simulator (SES) real-time simulation; and astronaut evaluation.

  18. Large space structure damping design

    NASA Technical Reports Server (NTRS)

    Pilkey, W. D.; Haviland, J. K.

    1983-01-01

    Several FORTRAN subroutines and programs were developed which compute complex eigenvalues of a damped system using different approaches, and which rescale mode shapes to unit generalized mass and make rigid bodies orthogonal to each other. An analytical proof of a Minimum Constrained Frequency Criterion (MCFC) for a single damper is presented. A method to minimize the effect of control spill-over for large space structures is proposed. The characteristic equation of an undamped system with a generalized control law is derived using reanalysis theory. This equation can be implemented in computer programs for efficient eigenvalue analysis or control quasi synthesis. Methods to control vibrations in large space structure are reviewed and analyzed. The resulting prototype, using electromagnetic actuator, is described.

  19. VIBRATION DAMPING AND SHOCK MOUNT

    DOEpatents

    Stevens, D.J.; Forman, G.W.

    1963-12-10

    A shock absorbing mount in which vibrations are damped by an interference fit between relatively movable parts of the mount is described. A pair of generally cup-shaped parts or members have skirt portions disposed in an oppositely facing nesting relationship with the skirt of one member frictionally engaging the skirt of the other. The outermost skirt may be slotted to provide spring-like segments which embrace the inner skirt for effecting the interference fit. Belleville washers between the members provide yieldable support for a load carried by the mount. When a resonant frequency of vibration forces acting upon the moumt attains a certain level the kinetic energy of these forces is absorbed by sliding friction between the parts. (AEC)

  20. Stabilization of ATF5 by TAK1–Nemo-Like Kinase Critically Regulates the Interleukin-1β-Stimulated C/EBP Signaling Pathway

    PubMed Central

    Zhang, Ze-Yan; Li, Shang-Ze; Zhang, Hui-Hui; Wu, Qu-Ran; Gong, Jun; Liang, Tong; Gao, Lu; Xing, Na-Na; Liu, Wen-Bin

    2014-01-01

    Interleukin-1β (IL-1β) is a key proinflammatory cytokine that initiates several signaling cascades, including those involving CCAAT/enhancer binding proteins (C/EBPs). The mechanism by which IL-1β propagates a signal that activates C/EBP has remained elusive. Nemo-like kinase (NLK) is a mitogen-activated protein kinase (MAPK)-like kinase associated with many pathways and phenotypes that are not yet well understood. Using a luciferase reporter screen, we found that IL-1β-induced C/EBP activation was positively regulated by NLK. Overexpression of NLK activated C/EBP and potentiated IL-1β-triggered C/EBP activation, whereas knockdown or knockout of NLK had the opposite effect. NLK interacted with activating transcription factor 5 (ATF5) and inhibited the proteasome-dependent degradation of ATF5 in a kinase-independent manner. Consistently, NLK deficiency resulted in decreased levels of ATF5. NLK cooperated with ATF5 to activate C/EBP, whereas NLK could not activate C/EBP upon knockdown of ATF5. Moreover, TAK1, a downstream effector of IL-1β that acts upstream of NLK, mimicked the ability of NLK to stabilize ATF5 and activate C/EBP. Thus, our findings reveal the TAK1-NLK pathway as a novel regulator of basal or IL-1β-triggered C/EBP activation though stabilization of ATF5. PMID:25512613

  1. Landau damping in a turbulent setting

    SciTech Connect

    Plunk, G. G.

    2013-03-15

    To address the problem of Landau damping in kinetic turbulence, we consider the forcing of the linearized Vlasov equation by a stationary random source. It is found that the time-asymptotic density response is dominated by resonant particle interactions that are synchronized with the source. The energy consumption of this response is calculated, implying an effective damping rate, which is the main result of this paper. Evaluating several cases, it is found that the effective damping rate can differ from the Landau damping rate in magnitude and also, remarkably, in sign. A limit is demonstrated in which the density and current become phase-locked, which causes the effective damping to be negligible; this result offers a fresh perspective from which to reconsider recent observations of kinetic turbulence satisfying critical balance.

  2. Damping characteristics of damaged fiber composite components

    NASA Technical Reports Server (NTRS)

    Eberle, K.

    1986-01-01

    Defects in fiber composite components produce changes with respect to the vibrational characteristics of the material. These changes can be recognized in the form of a frequency shift or an alteration of the damping process. The present investigation is concerned with questions regarding the possibility of a utilization of the changes in suitable defect-detecting inspection procedures. A description is given of a method for measuring the damping characteristics of a specimen. This method provides a spectrum of the damping coefficients of the sample as a basis for a comprehensive evaluation of the damping behavior. The correlation between defects and change in the damping characteristics is demonstrated with the aid of results obtained in measurements involving specimens of carbon-fiber composites and a component consisting of glass-fiber-reinforced plastics.

  3. Roles for ATF6 and the sarco/endoplasmic reticulum protein quality control system in the heart

    PubMed Central

    Glembotski, Christopher C.

    2014-01-01

    The hypertrophic growth of cardiac myocytes is a highly dynamic process that underlies physiological and pathological adaptation of the heart. Accordingly, a better understanding of the molecular underpinnings of cardiac myocyte hypertrophy is required in order to fully appreciate the causes and functional consequences of the changes in the size of the healthy and diseased heart. Hypertrophy is driven by increases in cardiacmyocyte protein, which must be balanced by cellular ability to maintain protein quality in order to avoid maladaptive accumulation of toxic misfolded proteins. Recent studies have shown that the endoplasmic reticulum (ER), which, in cardiac myocytes, comprises the sarco/endoplasmic reticulum (SR/ER), is the site of most protein synthesis. Thus, the protein quality control machinery located at the SR/ER is likely to be an important determinant of whether the heart responds adaptively to hypertrophic growth stimuli. The SR/ER-transmembrane protein, ATF6, serves a critical protein quality control function as a first responder to the accumulation of potentially toxic, misfolded proteins. Misfolded proteins transform ATF6 into a transcription factor that regulates a gene program that is partly responsible for enhancing protein quality control. Two ATF6-inducible genes that have been studied in the heart and shown to be adaptive are RCAN1 and Derl3, which encode proteins that decrease protein-folding demand, and enhance degradation of misfolded proteins, respectively. Thus, the ATF6-regulated SR/ER protein quality control system is important for maintaining protein quality during growth, making ATF6, and other components of the system, potentially attractive targets for the therapeutic management pathological cardiac hypertrophy. This article is part of a Special Issue entitled “Protein Quality Control, the Ubiquitin Proteasome System, and Autophagy”. PMID:24140798

  4. Calcium-independent phospholipase A2γ enhances activation of the ATF6 transcription factor during endoplasmic reticulum stress.

    PubMed

    Elimam, Hanan; Papillon, Joan; Takano, Tomoko; Cybulsky, Andrey V

    2015-01-30

    Injury of visceral glomerular epithelial cells (GECs) causes proteinuria in many glomerular diseases. We reported previously that calcium-independent phospholipase A2γ (iPLA2γ) is cytoprotective against complement-mediated GEC injury. Because iPLA2γ is localized at the endoplasmic reticulum (ER), this study addressed whether the cytoprotective effect of iPLA2γ involves the ER stress unfolded protein response (UPR). In cultured rat GECs, overexpression of the full-length iPLA2γ, but not a mutant iPLA2γ that fails to associate with the ER, augmented tunicamycin-induced activation of activating transcription factor-6 (ATF6) and induction of the ER chaperones, glucose-regulated protein 94 (GRP94) and glucose-regulated protein 78 (GRP78). Augmented responses were inhibited by the iPLA2γ inhibitor, (R)-bromoenol lactone, but not by the cyclooxygenase inhibitor, indomethacin. Tunicamycin-induced cytotoxicity was reduced in GECs expressing iPLA2γ, and the cytoprotection was reversed by dominant-negative ATF6. GECs from iPLA2γ knock-out mice showed blunted ATF6 activation and chaperone up-regulation in response to tunicamycin. Unlike ATF6, the two other UPR pathways, i.e. inositol-requiring enzyme 1α and protein kinase RNA-like ER kinase pathways, were not affected by iPLA2γ. Thus, in GECs, iPLA2γ amplified activation of the ATF6 pathway of the UPR, resulting in up-regulation of ER chaperones and cytoprotection. These effects were dependent on iPLA2γ catalytic activity and association with the ER but not on prostanoids. Modulating iPLA2γ activity may provide opportunities for pharmacological intervention in glomerular diseases associated with ER stress. PMID:25492867

  5. Selective activation of the transcription factor ATF6 mediates endoplasmic reticulum proliferation triggered by a membrane protein.

    PubMed

    Maiuolo, Jessica; Bulotta, Stefania; Verderio, Claudia; Benfante, Roberta; Borgese, Nica

    2011-05-10

    It is well known that the endoplasmic reticulum (ER) is capable of expanding its surface area in response both to cargo load and to increased expression of resident membrane proteins. Although the response to increased cargo load, known as the unfolded protein response (UPR), is well characterized, the mechanism of the response to membrane protein load has been unclear. As a model system to investigate this phenomenon, we have used a HeLa-TetOff cell line inducibly expressing a tail-anchored construct consisting of an N-terminal cytosolic GFP moiety anchored to the ER membrane by the tail of cytochrome b5 [GFP-b(5)tail]. After removal of doxycycline, GFP-b(5)tail is expressed at moderate levels (1-2% of total ER protein) that, nevertheless, induce ER proliferation, as assessed both by EM and by a three- to fourfold increase in phosphatidylcholine synthesis. We investigated possible participation of each of the three arms of the UPR and found that only the activating transcription factor 6 (ATF6) arm was selectively activated after induction of GFP-b(5)tail expression; peak ATF6α activation preceded the increase in phosphatidylcholine synthesis. Surprisingly, up-regulation of known ATF6 target genes was not observed under these conditions. Silencing of ATF6α abolished the ER proliferation response, whereas knockdown of Ire1 was without effect. Because GFP-b(5)tail lacks a luminal domain, the response we observe is unlikely to originate from the ER lumen. Instead, we propose that a sensing mechanism operates within the lipid bilayer to trigger the selective activation of ATF6. PMID:21521793

  6. Experimental Investigation of the Damping Behavior of the Particle Damping in the Transient Vibrations

    NASA Astrophysics Data System (ADS)

    Chavan, Shrirang Pandurang; Kale, Arvind Kamalakar; Mulla, Faiz Abdulkadar

    2016-01-01

    Particle damping is a non linear type of damping in which energy of the vibratory system is dissipated by the impact and the frictional losses made by the particles used for the damping purposes. The particle damping technique is useful over other types of damping as it is temperature independent. So it is reliable over wide temperature range and hence is essentially used in the cryogenic and the gas turbine related applications. For experimentation, cantilever beam with particle enclosure attached to its free end has been extensively used and the effect of the particle material, particle size, mass ratio and enclosure height on the damping performance has been studied [1]. For a small weight penalty, rather large amounts of damping can be achieved [2].

  7. Saturn's Spectacular Ring System

    NASA Technical Reports Server (NTRS)

    Lissauer, Jack J.; DeVincenzi, Donald (Technical Monitor)

    2001-01-01

    Saturn's beautiful rings have fascinated astronomers since they were first observed by Galileo in 1610. The main rings consist of solid particles mostly in the 1 cm - 10 m range, composed primarily of water ice. The ring disk is exceptionally thin - the typical local thickness of the bright rings is tens of meters, whereas the diameter of the main rings is 250,000 km! The main rings exhibit substantial radial variations "ringlets", many of which are actively maintained via gravitational perturbations from Saturn's moons. Exterior to the main rings lie tenuous dust rings, which have little mass but occupy a very large volume of space. This seminar will emphasize the physics of ring-moon interactions, recent advances in our understanding of various aspects of the rings obtained from observations taken during 1995 when the rings appeared edge-on to the Earth and then to the Sun, and observations in subsequent years from HST.

  8. Development of a thermal diode heat pipe for the advanced thermal control flight experiment /ATFE/.

    NASA Technical Reports Server (NTRS)

    Swerdling, B.; Kosson, R.; Urkowitz, M.; Kirkpatrick, J.

    1972-01-01

    Description of the analysis, design, fabrication, and test of the engineering model of the ATFE diode. Included is a review of several diode concepts that led to selection of the liquid blockage technique for shut-off. The diode is made of stainless steel, 26 in. long, 0.375-in. nominal OD, with self-filling spiral artery wick and ammonia working fluid. In the normal heat pipe mode, at ambient temperatures, the diode capacity is 85 W. For flight, the pipe will deliver 20 W with a 9 F temperature difference between the external evaporator and condenser surfaces. Reverse mode conduction is less than 1.5 W with a 260 F temperature difference.

  9. Plasma wakefields in the quasi-nonlinear regime: Experiments at ATF

    SciTech Connect

    Rosenzweig, J. B.; Andonian, G.; Barber, S.; Ferrario, M.; Muggli, P.; O'Shea, B.; Sakai, Y.; Valloni, A.; Williams, O.; Xi, Y.; Yakimenko, V.

    2012-12-21

    In this work we present details of planned experiments to investigate certain aspects of the quasi non linear regime (QNL) of plasma wakefield acceleration (PWFA). In the QNL regime it is, in principal, possible to combine the benefits of both nonlinear and linear PWFA. That is, beams of high quality can be maintained through acceleration due to the complete ejection of plasma electrons from beam occupied region, while large energy gains can be achieved through use of transformer ratio increasing schemes, such as ramped bunch trains. With the addition of an short focal length PMQ triplet capable of focusing beams to the few micron scale and the ability to generate tunable bunch trains, the Accelerator Test Facility (ATF) at Brookhaven National Lab offers the unique capabilities to probe these characteristics of the QNL regime.

  10. Active interlock for the NSLS-II damping wiggler

    NASA Astrophysics Data System (ADS)

    Choi, J.; Willeke, F.

    2012-07-01

    The NSLS-II is a 3rd generation light source with ultra-low beam emittance that is currently under construction at the Brookhaven National Laboratory. Because the power of the synchrotron radiation from the damping wiggler (DW) is about 64 kW, a slight mis-steer can result in severe damage to the vacuum chamber. To avoid such problems, an active interlock system is being considered. The system dumps the beam when it departs from the predefined safe window in the phase space. In this paper, we present simple geometric arguments from which we define the safe window on the basis of betatron amplitudes. This window can be applied to any DW around the ring. For the entrance of the wiggler, we obtained window of Δx=±8.4 mm, Δx'=±429 μrad and Δy=±2.1 mm, Δy'=±449 μrad.

  11. Stirling engine piston ring

    DOEpatents

    Howarth, Roy B.

    1983-01-01

    A piston ring design for a Stirling engine wherein the contact pressure between the piston and the cylinder is maintained at a uniform level, independent of engine conditions through a balancing of the pressure exerted upon the ring's surface and thereby allowing the contact pressure on the ring to be predetermined through the use of a preloaded expander ring.

  12. Validation Of Equivalent Viscous Damping Methodologies

    NASA Astrophysics Data System (ADS)

    Vaquer Araujo, Xavier; Fransen, S. H. J. A.; Germes, S.; Thiry, N.

    2012-07-01

    An important step in the design and verification process of spacecraft structures is the coupled dynamic analysis with the launch vehicle in the low-frequency domain. To obtain accurate predictions of the satellite’s dynamic environment it is essential that the damping of the system is correctly defined and taken into account within the resolution methodologies for the Coupled Loads Analysis (CLA). When working with finite element models, the materials’ damping is characterized by structural damping ratios. In addition, most of the load cases present in the CLA are transient excitations so the resolution of the equations of motion must be done in the time domain. Unfortunately, transient analyses cannot be carried out using structural damping models. Thus, a transformation from a structural to a viscous damping characterization is necessary. Nevertheless, this transformation is not trivial. There exist many methodologies aiming at computing an equivalent viscous damping matrix of the system so it can be used in transient analyses. This paper describes the results obtained in the validation of equivalent viscous damping methodologies used in the European Space Agency. This work permitted to identify the limitations of these methodologies and to come up with an enhanced methodology that predicts more reliable results.

  13. Bounce harmonic Landau damping of plasma waves

    NASA Astrophysics Data System (ADS)

    Anderegg, F.; Affolter, M.; Kabantsev, A. A.; Dubin, D. H. E.; Ashourvan, A.; Driscoll, C. F.

    2016-05-01

    We present measurements of bounce harmonic Landau damping due to z-variations in the plasma potential, created by an azimuthally symmetric "squeeze" voltage Vs applied to the cylindrical wall. Traditional Landau damping on spatially uniform plasma is weak in regimes where the wave phase velocity vp h≡ω/k is large compared to the thermal velocity. However, z-variations in plasma density and potential create higher spatial harmonics, which enable resonant wave damping by particles with bounce-averaged velocities vp h/n , where n is an integer. In our geometry, the applied squeeze predominantly generates a resonance at vp h/3 . Wave-coherent laser induced fluorescence measurements of particle velocities show a distinctive Landau damping signature at vp h/3 , with amplitude proportional to the applied Vs. The measured (small amplitude) wave damping is then proportional to Vs2 , in quantitative agreement with theory over a range of 20 in temperature. Significant questions remain regarding "background" bounce harmonic damping due to ubiquitous confinement fields and regarding the saturation of this damping at large wave amplitudes.

  14. Enhanced Damping for Capillary Bridge Oscillation Using Velocity Feedback

    NASA Technical Reports Server (NTRS)

    Wei, Wei; Thiessen, David B.; Marston, Philip L.

    2004-01-01

    The stability of cylindrical liquid bridges in reduced gravity is affected by ambient vibrations of the spacecraft. Such vibrations are expected to excite capillary modes of the bridge. The lowest-order unstable mode is particularly susceptible to vibration as the length of the bridge approaches the stability limit. This low-order mode is known as the (2,0) mode and is an axisymmetric varicose mode of one wavelength in the axial direction. In this work, an optical system is used to detect the (2,0)-mode amplitude. The derivative of the error signal produced by this detector is used to produce the appropriate voltages on a pair of ring electrodes which are concentric with the bridge. A mode-coupled Maxwell stress profile is thus generated in proportional to the modal velocity. Depending on the sign of the gain, the damping of the capillary oscillation can be either increased or decreased. This effect has been demonstrated in Plateau-tank experiments. Increasing the damping of the capillary modes on free liquid surfaces in space could be beneficial for containerless processing and other novel technologies. [work supported by NASA

  15. Enhanced Damping for Capillary Bridge Oscillation Using Velocity Feedback

    NASA Technical Reports Server (NTRS)

    Wei, Wei; Thiessen, David B.; Marston, Philip L.

    2004-01-01

    The stability of cylindrical liquid bridges in reduced gravity is affected by ambient vibrations of the spacecraft. Such vibrations are expected to excite capillary modes of the bridge. The lowest-order unstable mode is particularly susceptible to vibration as the length of the bridge approaches the stability limit. This low-order mode is known as the (2,0) mode and is an axisymmetric varicose mode of one wavelength in the axial direction. In this work, an optical system is used to detect the (2,0)-mode amplitude. The derivative of the error signal produced by this detector is used to produce the appropriate voltages on a pair of ring electrodes which are concentric with the bridge. A mode-coupled Maxwell stress profile is thus generated in proportional to the modal velocity. Depending on the sign of the gain, the damping of the capillary oscillation can be either increased or decreased. This effect has been demonstrated in Plateau-tank experiments. Increasing the damping of the capillary modes on free liquid surfaces in space could be beneficial for containerless processing and other novel technologies.

  16. Actin Rings of Power.

    PubMed

    Schwayer, Cornelia; Sikora, Mateusz; Slováková, Jana; Kardos, Roland; Heisenberg, Carl-Philipp

    2016-06-20

    Circular or ring-like actin structures play important roles in various developmental and physiological processes. Commonly, these rings are composed of actin filaments and myosin motors (actomyosin) that, upon activation, trigger ring constriction. Actomyosin ring constriction, in turn, has been implicated in key cellular processes ranging from cytokinesis to wound closure. Non-constricting actin ring-like structures also form at cell-cell contacts, where they exert a stabilizing function. Here, we review recent studies on the formation and function of actin ring-like structures in various morphogenetic processes, shedding light on how those different rings have been adapted to fulfill their specific roles. PMID:27326928

  17. New Dust Belts of Uranus: One Ring, Two Ring, Red Ring, Blue Ring

    SciTech Connect

    de Pater, I; Hammel, H B; Gibbard, S G; Showalter, M R

    2006-02-02

    We compare near-infrared observations of the recently discovered outer rings of Uranus with HST results. We find that the inner ring, R/2003 U 2, is red, whereas the outer ring, R/2003 U 1, is very blue. Blue is an unusual color for rings; Saturn's enigmatic E ring is the only other known example. By analogy to the E ring, R/2003 U 1 is probably produced via impacts into the embedded moon Mab, which apparently orbits at a location where non-gravitational perturbations favor the survival and spreading of sub-micron sized dust. R/2003 U 2 more closely resembles Saturn's G ring.

  18. Evolution of a Ring around the Pluto-Charon Binary

    NASA Astrophysics Data System (ADS)

    Bromley, Benjamin C.; Kenyon, Scott J.

    2015-08-01

    We consider the formation of satellites around the Pluto-Charon binary. An early collision between the two partners likely produced the binary and a narrow ring of debris, out of which arose the moons Styx, Nix, Kerberos, and Hydra. How the satellites emerged from the compact ring is uncertain. Here we show that a particle ring spreads from physical collisions and collective gravitational scattering, similar to migration. Around a binary, these processes take place in the reference frames of “most circular” orbits, akin to circular ones in a Keplerian potential. Ring particles damp to these orbits and avoid destructive collisions. Damping and diffusion also help particles survive dynamical instabilities driven by resonances with the binary. In some situations, particles become trapped near resonances that sweep outward with the tidal evolution of the Pluto-Charon binary. With simple models and numerical experiments, we show how the Pluto-Charon impact ring may have expanded into a broad disk, out of which grew the circumbinary moons. In some scenarios, the ring can spread well beyond the orbit of Hydra, the most distant moon, to form a handful of smaller satellites. If these small moons exist, New Horizons will find them.

  19. Continuum damping of ideal toroidal Alfven eigenmodes

    SciTech Connect

    Zhang, X.D.; Zhang, Y.Z.; Mahajan, S.M.

    1993-08-01

    A perturbation theory based on the two dimensional (2D) ballooning transform is systematically developed for ideal toroidal Alfven eigenmodes (TAEs). A formula, similar to the Fermi golden rule for decaying systems in quantum mechanics, is derived for the continuum damping rate of the TAE; the decay (damping) rate is expressed explicitly in terms of the coupling of the TAE to the continuum spectrum. Numerical results are compared with previous calculations. It is found that in some narrow intervals of the parameter m{cflx {epsilon}} the damping rate varies very rapidly. These regions correspond precisely to the root missing intervals of the numerical solution by Rosenbluth et al.

  20. A Resonant Damping Study Using Piezoelectric Materials

    NASA Technical Reports Server (NTRS)

    Min, J. B.; Duffy, K. P.; Choi, B. B.; Morrison, C. R.; Jansen, R. H.; Provenza, A. J.

    2008-01-01

    Excessive vibration of turbomachinery blades causes high cycle fatigue (HCF) problems requiring damping treatments to mitigate vibration levels. Based on the technical challenges and requirements learned from previous turbomachinery blade research, a feasibility study of resonant damping control using shunted piezoelectric patches with passive and active control techniques has been conducted on cantilever beam specimens. Test results for the passive damping circuit show that the optimum resistive shunt circuit reduces the third bending resonant vibration by almost 50%, and the optimum inductive circuit reduces the vibration by 90%. In a separate test, active control reduced vibration by approximately 98%.

  1. Saturation of Gyrokinetic Turbulence through Damped Eigenmodes

    SciTech Connect

    Hatch, D. R.; Terry, P. W.; Jenko, F.; Merz, F.; Nevins, W. M.

    2011-03-18

    In the context of toroidal gyrokinetic simulations, it is shown that a hierarchy of damped modes is excited in the nonlinear turbulent state. These modes exist at the same spatial scales as the unstable eigenmodes that drive the turbulence. The larger amplitude subdominant modes are weakly damped and exhibit smooth, large-scale structure in velocity space and in the direction parallel to the magnetic field. Modes with increasingly fine-scale structure are excited to decreasing amplitudes. In aggregate, damped modes define a potent energy sink. This leads to an overlap of the spatial scales of energy injection and peak dissipation, a feature that is in contrast with more traditional turbulent systems.

  2. Oscillation damped movement of suspended objects

    SciTech Connect

    Jones, J.F.; Petterson, B.J.

    1988-01-01

    Transportation of objects using overhead cranes or manipulators can induce pendulum motion of the object. Residual oscillation from transportation typically must be damped or allowed to decay before the next process can take place. By properly programming the acceleration of the transporting device (e.g., crane) an oscillation damped transport and swing free stop is obtainable. This paper reviews the theory associated with oscillation damped trajectories for simply suspended objects and describes a particular implementation using a CIMCORP XR 6100 gantry robot. 8 refs., 7 figs., 1 tab.

  3. Turbine blade friction damping study

    NASA Technical Reports Server (NTRS)

    Dominic, R. J.

    1985-01-01

    A lumped parameter method, implemented on a VAX 11/780 computer shows that the primary parameters affecting the performance of the friction damper of the first stage turbine of the SSME high pressure fuel pump are: the damper-blade coefficient of friction; the normal force applied to the friction interface; the amplitude of the periodic forcing function; the relative phase angle of the forcing functions for adjacent blades bridged by a damper (effectively, the engine order of the forcing function); and the amount of hysteretic damping that acts to limit the vibration amplitude of the blade in its resonance modes. The low order flexural resonance vibration modes of HPFTP blades without dampers, with production dampers, and with two types of lightweight experimental dampers were evaluated in high speed spin pit tests. Results agree with those of the analytical study in that blades fitted with production friction dampers experienced the airfoil-alone flexural resonance mode, while those without dampers or with lighter weight dampers did not. No blades fitted with dampers experienced the whole blade flexural resonance mode during high speed tests, while those without dampers did.

  4. Artificial Compressibility with Entropic Damping

    NASA Astrophysics Data System (ADS)

    Clausen, Jonathan; Roberts, Scott

    2012-11-01

    Artificial Compressibility (AC) methods relax the strict incompressibility constraint associated with the incompressible Navier-Stokes equations. Instead, they rely on an artificial equation of state relating pressure and density fluctuations through a numerical Mach number. Such methods are not new: the first AC methods date back to Chorin (1967). More recent applications can be found in the lattice-Boltzmann method, which is a kinetic/mesoscopic method that converges to an AC form of the Navier-Stokes equations. With computing hardware trending towards massively parallel architectures in order to achieve high computational throughput, AC style methods have become attractive due to their local information propagation and concomitant parallelizable algorithms. In this work, we examine a damped form of AC in the context of finite-difference and finite-element methods, with a focus on achieving time-accurate simulations. Also, we comment on the scalability of the various algorithms. Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.

  5. Turbulent Damping without Eddy Viscosity

    NASA Astrophysics Data System (ADS)

    Thalabard, Simon

    2015-11-01

    The intrinsic Non-Gaussianity of turbulence may explain why the standard Quasi-Normal cumulant discard closures can fail dramatically, an example being the development of negative energy spectra in Millionshtchikov's 1941 Quasi-Normal (QN) theory. While Orszag's 1977 EDQNM provides an ingenious patch to the issue, the reason why QN fails so badly is not so clear. Is it because of the Gaussian Ansatz itself? Or rather its inconsistent use? The purpose of the talk is to argue in favor of the latter option, using the lights of a new ``optimal closure'' recently exposed by [Turkington,2013], which allows Gaussians to be used consistently with an intrinsic damping. The key to this apparent paradox lies in a clear distinction between the ensemble averages and their proxies, most easily grasped provided one uses the Liouville equation rather than the cumulant hierarchy as a starting point. Schematically said, closure is achieved by minimizing a lack-of-fit residual, that retains the intrinsic features of the dynamics. For the sake of clarity, I will discuss the optimal closure on a problem where it can be entirely implemented and compared to DNS: the relaxation of an arbitrarily far from equilibrium energy shell towards the Gibbs equilibrium for truncated Euler dynamics.

  6. Eigensolutions of non-proportionally damped systems based on continuous damping sensitivity

    NASA Astrophysics Data System (ADS)

    Lázaro, Mario

    2016-02-01

    The viscous damping model has been widely used to represent dissipative forces in structures under mechanical vibrations. In multiple degree of freedom systems, such behavior is mathematically modeled by a damping matrix, which in general presents non-proportionality, that is, it does not become diagonal in the modal space of the undamped problem. Eigensolutions of non-proportional systems are usually estimated assuming that the modal damping matrix is diagonally dominant (neglecting the off-diagonal terms) or, in the general case, using the state-space approach. In this paper, a new closed-form expression for the complex eigenvalues of non-proportionally damped system is proposed. The approach is derived assuming small damping and involves not only the diagonal terms of the modal damping matrix, but also the off-diagonal terms, which appear under higher order. The validity of the proposed approach is illustrated through a numerical example.

  7. Structuring eccentric-narrow planetary rings

    NASA Astrophysics Data System (ADS)

    Papaloizou, J. C. B.; Melita, M. D.

    2005-06-01

    A simple and general description of the dynamics of a narrow-eccentric ring is presented. We view an eccentric ring which precesses uniformly at a slow rate as exhibiting a global m=1 mode, which can be seen as originating from a standing wave superposed on an axisymmetric background. We adopt a continuum description using the language of fluid dynamics which gives equivalent results for the secular dynamics of thin rings as the well-known description in terms of a set of discrete elliptical streamlines formulated by Goldreich and Tremaine (1979, Astron. J. 84, 1638-1641). We use this to discuss the nonlinear mode interactions that appear in the ring through the excitation of higher m modes because of the coupling of the m=1 mode with an external satellite potential, showing that they that can lead to the excitation of the m=1 mode through a feedback process. In addition to the external perturbations by neighboring satellites, our model includes effects due to inelastic inter-particle collisions. Two main conditions for the ring to be able to maintain a steady m=1 normal mode are obtained. One can be expressed as an integral condition for the normal mode pattern to precess uniformly, which requires the correct balance between the differential precession induced by the oblateness of the central planet, self-gravity and collisional effects is the continuum form of that obtained from the N streamline model of Goldreich and Tremaine (1979, Astron. J. 84, 1638-1641). The other condition, not before examined in detail, is for the steady maintenance of the nonzero radial action that the ring contains because of its finite normal mode. This requires a balance between injection due to eccentric resonances arising from external satellites and additional collisional damping associated with the presence of the m=1 mode. We estimate that such a balance can occur in the ɛ-ring of Uranus, given its currently observed physical and orbital parameters.

  8. Linear collisionless Landau damping in Hilbert space

    NASA Astrophysics Data System (ADS)

    Zocco, Alessandro

    2015-08-01

    The equivalence between the Laplace transform (Landau, J. Phys. USSR 10 (1946), 25) and Hermite transform (Zocco and Schekochihin, Phys. Plasmas 18, 102309 (2011)) solutions of the linear collisionless Landau damping problem is proven.

  9. Nucleon exchange in damped nuclear reactions

    SciTech Connect

    Randrup, J.

    1986-04-01

    Starting from the general context of one-body nuclear dynamics, the nucleon-exchange mechanism in damped nuclear reactions is discussed. Some of its characteristic effects on various dinuclear observables are highlighted and a few recent advances are described.

  10. Magnetic Damping in Ferromagnetic Thin Films

    NASA Astrophysics Data System (ADS)

    Oogane, Mikihiko; Wakitani, Takeshi; Yakata, Satoshi; Yilgin, Resul; Ando, Yasuo; Sakuma, Akimasa; Miyazaki, Terunobu

    2006-05-01

    We determined the Gilbert damping constants of Fe-Co-Ni and Co-Fe-B alloys with various compositions and half-metallic Co2MnAl Heusler alloy films prepared by magnetron sputtering. The ferromagnetic resonance (FMR) technique was used to determine the damping constants of the prepared films. The out-of-plane angular dependences of the resonance field (HR) and line width (Δ Hpp) of FMR spectra were measured and fitted using the Landau-Lifshitz-Gilbert (LLG) equation. The experimental results fitted well, considering the inhomogeneities of the films in the fitting. The damping constants of the metallic films were much larger than those of bulk ferrimagnetic insulators and were roughly proportional to (g-2)2, where g is the Lande g factor. We discuss the origin of magnetic damping, considering spin-orbit and s-d interactions.

  11. Concepts and effects of damping in isolators

    NASA Technical Reports Server (NTRS)

    Kerley, J.

    1984-01-01

    A series of innovative designs and inventions which led to the solution of many aerospace vibration and shock problems through damping techniques is presented. The design of damped airborne structures has presented a need for such creative innovation. The primary concern was to discover what concepts were necessary for good structural damping. Once these concepts are determined and converted into basic principles, the design of hardware follows. The following hardware and techniques were developed in support of aerospace program requirements: shipping containers, alignment cables for precision mechanisms, isolation of small components such as relays and flight instruments, isolation for heavy flight equipment, coupling devices, universal joints, use of wire mesh to replace cable, isolation of 16-dB, 5000 lb horn, and compound damping devices to get better isolation from shock and vibration in a high steady environment.

  12. Simplified Model of Nonlinear Landau Damping

    SciTech Connect

    N. A. Yampolsky and N. J. Fisch

    2009-07-16

    The nonlinear interaction of a plasma wave with resonant electrons results in a plateau in the electron distribution function close to the phase velocity of the plasma wave. As a result, Landau damping of the plasma wave vanishes and the resonant frequency of the plasma wave downshifts. However, this simple picture is invalid when the external driving force changes the plasma wave fast enough so that the plateau cannot be fully developed. A new model to describe amplification of the plasma wave including the saturation of Landau damping and the nonlinear frequency shift is proposed. The proposed model takes into account the change of the plasma wave amplitude and describes saturation of the Landau damping rate in terms of a single fluid equation, which simplifies the description of the inherently kinetic nature of Landau damping. A proposed fluid model, incorporating these simplifications, is verified numerically using a kinetic Vlasov code.

  13. Coarse-graining Landau-Lifshitz damping

    NASA Astrophysics Data System (ADS)

    Feng, Xuebing; Visscher, P. B.

    2001-06-01

    High speed switching in magnetic materials is usually studied with the Landau-Lifshitz (LL) equation, which describes damping through a phenomenological coefficient. The results of micromagnetic calculations based on the LL equation have been observed to depend strongly on the cell size. We take a coarse-graining or renormalization-group approach to this cell size dependence: from a simulation using cell size L, we look at the dynamics of a cell of size 2L and determine an effective damping coefficient that describes the larger-scale dynamics. This can be thought of as a Green-Kubo calculation of the effective damping coefficient. In principle, this makes it possible to coarse grain from the atomic scale to determine the micromagnetic damping coefficient.

  14. Oscillation damping means for magnetically levitated systems

    DOEpatents

    Post, Richard F.

    2009-01-20

    The present invention presents a novel system and method of damping rolling, pitching, or yawing motions, or longitudinal oscillations superposed on their normal forward or backward velocity of a moving levitated system.

  15. Active damping and compensation of satellite appendages

    NASA Astrophysics Data System (ADS)

    Charon, W.; Baier, H.

    1993-10-01

    Future space missions will employ large and, for lightweight reasons, extremely flexible structures with very high performance requirements such as high pointing accuracy and stability, and high shape precision. This requires actively damping out vibrations induced by spacecraft maneuvers. The damping of the solar array vibrations is a characteristic task for such active interface devices. The example of an active interface for damping the bending vibrations of large conventional solar arrays is addressed. Other typical active components are active tube sections for damping the vibrations of large booms, and interfaces between satellite and vibrating large masts carrying high precision reflectors or measurement systems. The mechanical properties of the interfaces and the technological requirements related to their development are determined. New 'smart' materials are prominent among current concerns. Piezoelectric polymer foils bonded to structural shell surfaces, embedded thin piezoceramics plates, and embedded fiber optics sensors, as well as the implementation of materials such as memory alloys, are here addressed.

  16. Random vibrations of quadratic damping systems. [optimum damping analysis for automobile suspension system

    NASA Technical Reports Server (NTRS)

    Sireteanu, T.

    1974-01-01

    An oscillating system with quadratic damping subjected to white noise excitation is replaced by a nonlinear, statistically equivalent system for which the associated Fokker-Planck equation can be exactly solved. The mean square responses are calculated and the optimum damping coefficient is determined with respect to the minimum mean square acceleration criteria. An application of these results to the optimization of automobile suspension damping is given.

  17. The Roles of ATF3, an Adaptive-Response Gene, in High-Fat-Diet-Induced Diabetes and Pancreatic β-Cell Dysfunction

    PubMed Central

    Zmuda, Erik J.; Qi, Ling; Zhu, Michael X.; Mirmira, Raghavendra G.; Montminy, Marc R.; Hai, Tsonwin

    2010-01-01

    Most people with type 2 diabetes (T2D) have reduced β-cell mass, and apoptosis is a key factor for this reduction. Previously, we showed that ATF3, an adaptive-response gene, is induced by various stress signals relevant to T2D, such as high glucose and high fatty acid. Because ATF3 is proapoptotic in β-cells, we tested the hypothesis that ATF3 plays a detrimental role and contributes to the development of T2D. We compared wild-type (WT) and ATF3 knockout (KO) mice in an animal model for T2D, high-fat diet-induced diabetes. We also used INS-1 β-cells and primary islets to analyze the roles of ATF3 in β-cell function, including insulin gene expression and glucose-induced insulin secretion. Surprisingly, WT mice performed better in glucose tolerance test than KO mice, suggesting a protective, rather than detrimental, role of ATF3. At 12 wk on high-fat diet, no β-cell apoptosis was observed, and the WT and KO mice had comparable β-cell areas. However, ATF3 deficiency significantly reduced serum insulin levels in the KO mice without affecting insulin sensitivity, suggesting reduced β-cell function in the KO mice. Analyses using INS-1 cells and primary islets support the notion that this defect is due, at least partly, to reduced insulin gene transcription in the KO islets without detectable reduction in glucose-induced calcium influx, a critical step for insulin secretion. In conclusion, our results support a model in which, before apoptosis becomes obvious, expression of ATF3 can be beneficial by helping β-cells to cope with higher metabolic demand. PMID:20519332

  18. Optimizing Thomson's jumping ring

    NASA Astrophysics Data System (ADS)

    Tjossem, Paul J. H.; Brost, Elizabeth C.

    2011-04-01

    The height to which rings will jump in a Thomson jumping ring apparatus is the central question posed by this popular lecture demonstration. We develop a simple time-averaged inductive-phase-lag model for the dependence of the jump height on the ring material, its mass, and temperature and apply it to measurements of the jump height for a set of rings made by slicing copper and aluminum alloy pipe into varying lengths. The data confirm a peak jump height that grows, narrows, and shifts to smaller optimal mass when the rings are cooled to 77 K. The model explains the ratio of the cooled/warm jump heights for a given ring, the reduction in optimal mass as the ring is cooled, and the shape of the mass resonance. The ring that jumps the highest is found to have a characteristic resistance equal to the inductive reactance of the set of rings.

  19. Quantum damped oscillator I: Dissipation and resonances

    SciTech Connect

    Chruscinski, Dariusz

    2006-04-15

    Quantization of a damped harmonic oscillator leads to so called Bateman's dual system. The corresponding Bateman's Hamiltonian, being a self-adjoint operator, displays the discrete family of complex eigenvalues. We show that they correspond to the poles of energy eigenvectors and the corresponding resolvent operator when continued to the complex energy plane. Therefore, the corresponding generalized eigenvectors may be interpreted as resonant states which are responsible for the irreversible quantum dynamics of a damped harmonic oscillator.

  20. Effect of material damping on bone remodelling.

    PubMed

    Misra, J C; Samanta, S

    1987-01-01

    This paper considers the effect of internal material damping on the stresses, strains, and surface and internal remodelling behaviour in a section of axisymmetrical bone with a force-fitted axially oriented medullary pin. The bone response to several loading situations is modelled using visco-elastic equations. An approximate method is developed to analyse the proposed mathematical model. By considering a numerical example, the effect of material damping on the remodelling stresses is quantified. PMID:3584150

  1. Spatial versus time hysteresis in damping mechanisms

    NASA Technical Reports Server (NTRS)

    Banks, H. T.; Fabiano, R. H.; Wang, Y.; Inman, D. J.; Cudney, H., Jr.

    1988-01-01

    A description is given of continuing investigations on the task of estimating internal damping mechanisms in flexible structures. Specifically, two models for internal damping in Euler-Bernoulli beams are considered: spatial hysteresis and time hysteresis. A theoretically sound computational algorithm for estimation is described, and experimental results are discussed. It is concluded that both models perform well in the sense that they accurately predict response for the experiments conducted.

  2. Turbine blade with tuned damping structure

    SciTech Connect

    Campbell, Christian X.; Messmann, Stephen J.

    2015-09-01

    A turbine blade is provided comprising: a root; an airfoil comprising an external wall extending radially from the root and having a radially outermost portion; and a damping structure. The external wall may comprise first and second side walls joined together to define an inner cavity of the airfoil. The damping structure may be positioned within the airfoil inner cavity and coupled to the airfoil so as to define a tuned mass damper.

  3. Modelling of Resonantly Forced Density Waves in Dense Planetary Rings

    NASA Astrophysics Data System (ADS)

    Lehmann, M.; Schmidt, J.; Salo, H.

    2014-04-01

    Density wave theory, originally proposed to explain the spiral structure of galactic disks, has been applied to explain parts of the complex sub-structure in Saturn's rings, such as the wavetrains excited at the inner Lindblad resonances (ILR) of various satellites. The linear theory for the excitation and damping of density waves in Saturn's rings is fairly well developed (e.g. Goldreich & Tremaine [1979]; Shu [1984]). However, it fails to describe certain aspects of the observed waves. The non-applicability of the linear theory is already indicated by the "cusplike" shape of many of the observed wave profiles. This is a typical nonlinear feature which is also present in overstability wavetrains (Schmidt & Salo [2003]; Latter & Ogilvie [2010]). In particular, it turns out that the detailed damping mechanism, as well as the role of different nonlinear effects on the propagation of density waves remain intransparent. First attemps are being made to investigate the excitation and propagation of nonlinear density waves within a hydrodynamical formalism, which is also the natural formalism for describing linear density waves. A simple weakly nonlinear model, derived from a multiple-scale expansion of the hydrodynamic equations, is presented. This model describes the damping of "free" spiral density waves in a vertically integrated fluid disk with density dependent transport coefficients, where the effects of the hydrodynamic nonlinearities are included. The model predicts that density waves are linearly unstable in a ring region where the conditions for viscous overstability are met, which translates to a steep dependence of the shear viscosity with respect to the disk's surface density. The possibility that this dependence could lead to a growth of density waves with increasing distance from the resonance, was already mentioned in Goldreich & Tremaine [1978]. Sufficiently far away from the ILR, the surface density perturbation caused by the wave, is predicted to

  4. The first terawatt picosecond CO{sub 2} laser for advanced accelerator studies at the Brookhaven ATF

    SciTech Connect

    Pogorelsky, I.V.; Ben-Zvi, I.; Skaritka, J.

    1996-10-01

    The first terawatt picosecond C0{sub 2} laser system is under development at the Brookhaven Accelerator Test Facility. Presently operational 1 Joule 100-ps ATF laser will be upgraded with a 10 atm amplifier capable of delivery {approximately} 15 Joules of laser energy in a 3 ps pulse. We describe the design of the x-ray preionized 10 atm amplifier of a 10 liter active volume energized by a 1 MV, 200 kA transverse electric discharge. The amplifier, equipped with internal optics, permits the accommodation of a regenerative stage and a multi-pass booster in a relatively compact single discharge volume. The ATF terawatt C0{sub 2} laser shall become operational in 1997 to serve for laser acceleration, x-ray generation and other strong-field physics experiments.

  5. Embedded absorbers for helicopter rotor lag damping

    NASA Astrophysics Data System (ADS)

    Byers, Lynn; Gandhi, Farhan

    2009-09-01

    Radial and chordwise damped vibration absorbers embedded in the rotor blade are compared for rotor lag damping augmentation. Results show that the radial absorber is more effective in transferring damping to the rotor blade lag mode. The chordwise absorber needs to be at a more outboard location and have a larger mass to introduce levels of lag damping comparable to that introduced by the radial absorber. The 1/rev amplitude of a chordwise absorber at the blade tip, per degree of blade lead-lag motion in forward flight, is of the order of 35% of the blade chord, and such a stroke might be difficult to accommodate. The 1/rev amplitude of a radial absorber at 70% span (having significantly lower mass than the chordwise absorber and producing comparable lag damping) is of the order of 4% of the rotor blade span. The static displacement of the radial absorber under centrifugal load needs to be limited using a frequency-dependent (high static stiffness, low dynamic stiffness) or nonlinear spring. The chordwise absorber can also undergo a large static displacement under the chordwise component of the centrifugal load if there is an offset from the feather axis, and this would again have to be limited using a strategy such as a frequency-dependent spring. Significant advantages of the radial absorber are—higher lag damping, lower absorber mass, space for absorber mass travel, and no chordwise travel of blade center of gravity reducing susceptibility to aeroelastic instability and dynamic pitch-link loads.

  6. Damping of Sound Waves in Strong Centrifugal Field

    NASA Astrophysics Data System (ADS)

    Bogovalov, S. V.; Kislov, V. A.; Tronin, I. V.

    A method for numerical calculation of the sound wave damping and dispersion law in a strong centrifugal field of the order of 106 g is considered. The damping is defined from the width of the resonance peak for different wave vectors. In the strong centrifugal field damping of the sound waves essentially exceeds the damping in the quiescent gas.

  7. Jupiter's Main Ring/Ring Halo

    NASA Technical Reports Server (NTRS)

    1997-01-01

    A mosaic of four images taken through the clear filter (610 nanometers) of the solid state imaging (CCD) system aboard NASA's Galileo spacecraft on November 8, 1996, at a resolution of approximately 46 kilometers (28.5 miles) per picture element (pixel) along Jupiter's rings. Because the spacecraft was only about 0.5 degrees above the ring plane, the image is highly foreshortened in the vertical direction. The images were obtained when Galileo was in Jupiter's shadow, peering back toward the Sun; the ring was approximately 2.3 million kilometers (1.4 million miles) away. The arc on the far right of the image is produced when sunlight is scattered by small particles comprising Jupiter's upper atmospheric haze. The ring also efficiently scatters light, indicating that much of its brightness is due to particles that are microns or less in diameter. Such small particles are believed to have human-scale lifetimes, i.e., very brief compared to the solar system's age.

    Jupiter's ring system is composed of three parts - - a flat main ring, a lenticular halo interior to the main ring, and the gossamer ring, outside the main ring. The near and far arms of Jupiter's main ring extend horizontally across the mosaic, joining together at the ring's ansa, on the figure's far left side. The near arm of the ring appears to be abruptly truncated close to the planet, at the point where it passes into Jupiter's shadow. Some radial structure is barely visible across the ring's ansa (top image). A faint mist of particles can be seen above and below the main rings. This vertically extended 'halo' is unusual in planetary rings, and is probably caused by electromagnetic forces pushing the smallest grains out of the ring plane. Because of shadowing, the halo is not visible close to Jupiter in the lower right part of the mosaic. To accentuate faint features in the bottom image of the ring halo, different brightnesses are shown through color. Brightest features are white or yellow and the

  8. Landau damping in relativistic plasmas

    NASA Astrophysics Data System (ADS)

    Young, Brent

    2016-02-01

    We examine the phenomenon of Landau damping in relativistic plasmas via a study of the relativistic Vlasov-Poisson (rVP) system on the torus for initial data sufficiently close to a spatially uniform steady state. We find that if the steady state is regular enough (essentially in a Gevrey class of degree in a specified range) and if the deviation of the initial data from this steady state is small enough in a certain norm, the evolution of the system is such that its spatial density approaches a uniform constant value quasi-exponentially fast (i.e., like exp ( - C |" separators=" t | ν ¯ ) for ν ¯ ∈ ( 0 , 1 ) ). We take as a priori assumptions that solutions launched by such initial data exist for all times (by no means guaranteed with rVP, but a reasonable assumption since we are close to a spatially uniform state) and that the various norms in question are continuous in time (which should be a consequence of an abstract version of the Cauchy-Kovalevskaya theorem). In addition, we must assume a kind of "reverse Poincaré inequality" on the Fourier transform of the solution. In spirit, this assumption amounts to the requirement that there exists 0 < ϰ < 1 so that the mass in the annulus ϰ ≤ |" separators=" v | < 1 for the solution launched by the initial data is uniformly small for all t. Typical velocity bounds for solutions to rVP launched by small initial data (at least on ℝ6) imply this bound. We note that none of our results require spherical symmetry (a crucial assumption for many current results on rVP).

  9. On semi ring bornologies

    NASA Astrophysics Data System (ADS)

    Imran, A. N.; Rakhimov, I. S.; Husain, Sh. K. Said

    2016-06-01

    Our main focus in this work is to introduce new structure bornological semi rings. This generalizes the theory of algebraic semi rings from the algebraic setting to the framework of bornological sets. We give basic properties for this new structure. As well as, We study the fundamental construction of bornological semi ring as product, inductive limits and projective limits and their extensions on bornological semi ring. Additionally, we introduce the category of bornological semi rings and study product and pullback (fiber product) in the category of bornological semi rings.

  10. Docosahexaenoic acid consumption inhibits deoxynivalenol-induced CREB/ATF1 activation and IL-6 gene transcription in mouse macrophages.

    PubMed

    Jia, Qunshan; Zhou, Hui-Ren; Shi, Yuhui; Pestka, James J

    2006-02-01

    The mycotoxin deoxynivalenol (DON) induces IgA nephropathy in mice by upregulating IL-6 expression, which is suppressed by (n-3) PUFA consumption. The purpose of this study was to test the hypothesis that consumption of the (n-3) PUFA docosahexaenoic acid (DHA) interferes with DON-induced transcriptional and post-transcriptional upregulation of IL-6 mRNA in murine macrophages. DON evoked expression of IL-6 mRNA and IL-6 heterogenous nuclear RNA (hnRNA), an indicator of ongoing IL-6 transcription, in macrophages elicited from mice fed control AIN-93G diet for 4 wk, whereas expression of both RNA species was suppressed in macrophages from mice fed AIN-93G modified to contain 30 g DHA/kg diet for the same time period. DON enhanced IL-6 mRNA stability similarly in macrophages from control and DHA-fed mice suggesting that (n-3) PUFA effects were not post-transcriptional. DON upregulated binding activity of cAMP response element binding protein (CREB) and activator protein (AP-1) to their respective consensus sequences in nuclear extracts from control-fed mice, whereas both activities were suppressed in nuclear extracts from DHA-fed mice. DON induced phosphorylation of CREB at Ser-133 and ATF1 at Ser-63 as well as intranuclear binding of phospho-CREB/ATF1 to the cis element of the IL-6 promoter in control macrophages, whereas both activities were inhibited in macrophages from DHA-fed mice. DHA consumption blocked DON-induced phosphorylation of the CREB kinase AKT. Inhibition of AKT suppressed both CREB/ATF1 phosphorylation and IL-6 transcription. These data suggest that DHA consumption suppresses DON-induced IL-6 transcription in macrophages in part by interfering with AKT-dependent phosphorylation and subsequent binding of CREB/ATF1 to the IL-6 promoter. PMID:16424113

  11. Inhibin beta E is upregulated by drug-induced endoplasmic reticulum stress as a transcriptional target gene of ATF4

    SciTech Connect

    Brüning, Ansgar Matsingou, Christina; Brem, German Johannes; Rahmeh, Martina; Mylonas, Ioannis

    2012-10-15

    Inhibins and activins are gonadal peptide hormones of the transforming growth factor-β super family with important functions in the reproductive system. By contrast, the recently identified inhibin βE subunit, primarily expressed in liver cells, appears to exert functions unrelated to the reproductive system. Previously shown downregulation of inhibin βE in hepatoma cells and anti-proliferative effects of ectopic inhibin βE overexpression indicated growth-regulatory effects of inhibin βE. We observed a selective re-expression of the inhibin βE subunit in HepG2 hepatoblastoma cells, MCF7 breast cancer cells, and HeLa cervical cancer cells under endoplasmic reticulum stress conditions induced by tunicamycin, thapsigargin, and nelfinavir. Analysis of XPB1 splicing and ATF4 activation revealed that inhibin βE re-expression was associated with induction of the endoplasmic reticulum stress reaction by these drugs. Transfection of an ATF4 expression plasmid specifically induced inhibin βE expression in HeLa cells and indicates inhibin βE as a hitherto unidentified target gene of ATF4, a key transcription factor of the endoplasmic reticulum stress response. Therefore, the inhibin βE subunit defines not only a new player but also a possible new marker for drug-induced endoplasmic reticulum stress. -- Highlights: ► Endoplasmic reticulum stress induces inhibin beta E expression. ► Inhibin beta E is regulated by the transcription factor ATF4. ► Inhibin beta E expression can be used as a marker for drug-induced ER stress.

  12. Letter Report Documenting Progress of Second Generation ATF FeCrAl Alloy Fabrication

    SciTech Connect

    Yamamoto, Y.; Yang, Y.; Field, K. G.; Terrani, K.; Pint, B. A.; Snead, L. L.

    2014-06-10

    Development of the 2nd generation ATF FeCrAl alloy has been initiated, and a candidate alloy was selected for trial tube fabrication through hot-extrusion and gun-drilling processes. Four alloys based on Fe-13Cr-4.5Al-0.15Y in weight percent were newly cast with minor alloying additions of Mo, Si, Nb, and C to promote solid-solution and second-phase precipitate strengthening. The alloy compositions were selected with guidance from computational thermodynamic tools. The lab-scale heats of ~ 600g were arc-melted and drop-cast, homogenized, hot-forged and -rolled, and then annealed producing plate shape samples. An alloy with Mo and Nb additions (C35MN) processed at 800°C exhibits very fine sub-grain structure with the sub-grain size of 1-3μm which exhibited more than 25% better yield and tensile strengths together with decent ductility compared to the other FeCrAl alloys at room temperature. It was found that the Nb addition was key to improving thermal stability of the fine sub-grain structure. Optimally, grains of less than 30 microns are desired, with grains up to and order of magnitude in desired produced through Nb addition. Scale-up effort of the C35MN alloy was made in collaboration with a commercial cast company who has a capability of vacuum induction melting. A 39lb columnar ingot with ~81mm diameter and ~305mm height (with hot-top) was commercially cast, homogenized, hot-extruded, and annealed providing 10mm-diameter bar-shape samples with the fine sub-grain structure. This commercial heat proved consistent with materials produced at ORNL at the lab-scale. Tubes and end caps were machined from the bar sample and provided to another work package for the ATF-1 irradiation campaign in the milestone M3FT-14OR0202251.

  13. Hybrid Damping System for an Electronic Equipment Mounting Shelf

    NASA Technical Reports Server (NTRS)

    Voracek, David; Kolkailah, Faysal A.; Cavalli, J. R.; Elghandour, Eltahry

    1997-01-01

    The objective of this study was to design and construct a vibration control system for an electronic equipment shelf to be evaluated in the NASA Dryden FTF-11. The vibration control system was a hybrid system which included passive and active damping techniques. Passive damping was fabricated into the equipment shelf using ScothDamp(trademark) damping film and aluminum constraining layers. Active damping was achieved using a two channel active control circuit employing QuickPack(trademark) sensors and actuators. Preliminary Chirp test results indicated passive damping smoothed the frequency response while active damping reduced amplitudes of the frequency response for most frequencies below 500Hz.

  14. Hybrid Damping System for an Electronic Equipment Mounting Shelf

    NASA Technical Reports Server (NTRS)

    Voracek, David; Kolkailah, Faysal A.; Cavalli, J. R.; Elghandour, Eltahry

    1997-01-01

    The objective of this study was to design and construct a vibration control system for an electronic equipment shelf to be evaluated in the NASA Dryden FTF-II. The vibration control system was a hybrid system which included passive and active damping techniques. Passive damping was fabricated into the equipment shelf using ScothDamp(trademark) damping film and aluminum constraining layers. Active damping was achieved using a two channel active control circuit employing QuickPack(trademark) sensors and actuators. Preliminary Chirp test results indicated passive damping smoothed the frequency response while active damping reduced amplitudes of the frequency response for most frequencies below 500Hz.

  15. Mmu-miR-702 functions as an anti-apoptotic mirtron by mediating ATF6 inhibition in mice.

    PubMed

    Zhang, Wei-Guang; Chen, Lin; Dong, Qin; He, Juan; Zhao, Han-Dong; Li, Feng-Lan; Li, Hui

    2013-12-01

    MicroRNAs (miRNAs) are a group of endogenous, small, noncoding RNAs that function as key post-transcriptional regulators. miRNAs are involved in many biological processes including apoptosis. In this study, mouse miR-702 (mmu-miR-702), a mirtron derived from the 13th intron of the Plod3 gene, was identified as a regulator of anti-apoptosis. mmu-miR-702 was down-regulated after treatment with the apoptosis-inducer isoproterenol both in vivo and in vitro. According to over-expression experiments, mmu-miR-702 inhibited apoptosis as well as the expression levels of a subset of apoptosis-related genes including activating transcription factor 6 (ATF6). An interaction between mmu-miR-702 and the ATF6 3'-UTR binding site was confirmed using luciferase reporter and western blot assays. This is the first report of ATF6 interaction with miRNA. Although the possible existence of miR-702 in the human genome is low, our results indicate that mirtrons also participate in the process of apoptosis and may provide a novel study strategy for apoptosis. PMID:24035931

  16. A Project to Design and Build the Magnets for a New Test Beamline, the ATF2, at KEK

    SciTech Connect

    Spencer, Cherrill M.; Sugahara, Ryuhei; Masuzawa, Mika; Bolzon, Benoit; Jeremie, Andrea; /Annecy, LAPP

    2011-02-07

    In order to achieve the high luminosity required at the proposed International Linear Collider (ILC), it is critical to focus the beams to nanometer size with the ILC Beam Delivery System, and to maintain the beams collisions with a nanometer-scale stability. To establish the technologies associated with this ultra-high precision beam handling, a special beamline has been designed and built as an extension of the existing extraction beamline of the Accelerator Test Facility at KEK, Japan. The ATF provides an adequate ultra-low emittance electron beam that is comparable to the ILC requirements; the ATF2 mimics the ILC final focus system to create a tightly focused, stable beam. There are 37 magnets in the ATF2, 29 quadrupoles, 5 sextupoles and 3 bends. These magnets had to be acquired in a short time and at minimum cost, which led to various acquisition strategies; but nevertheless they had to meet strict requirements on integrated strength, physical dimensions, compatibility with existing magnet movers and beam position monitors, mechanical stability and field stability and quality. This paper will describe how 2 styles of quadrupoles, 2 styles of sextupoles, one dipole style and their supports were designed, fabricated, refurbished or modified, measured and aligned by a small team of engineers from 3 continents.

  17. Non-Linear Dynamics of Saturn's Rings

    NASA Astrophysics Data System (ADS)

    Esposito, L. W.

    2015-10-01

    Non-linear processes can explain why Saturn's rings are so active and dynamic. Ring systems differ from simple linear systems in two significant ways: 1. They are systems of granular material: where particle-to-particle collisions dominate; thus a kinetic, not a fluid description needed. We find that stresses are strikingly inhomogeneous and fluctuations are large compared to equilibrium. 2. They are strongly forced by resonances: which drive a non-linear response, pushing the system across thresholds that lead to persistent states. Some of this non-linearity is captured in a simple Predator-Prey Model: Periodic forcing from the moon causes streamline crowding; This damps the relative velocity, and allows aggregates to grow. About a quarter phase later, the aggregates stir the system to higher relative velocity and the limit cycle repeats each orbit, with relative velocity ranging from nearly zero to a multiple of the orbit average: 2-10x is possible. Results of driven N-body systems by Stuart Robbins: Even unforced rings show large variations; Forcing triggers aggregation; Some limit cycles and phase lags seen, but not always as predicted by predator-prey model. Summary of Halo Results: A predatorprey model for ring dynamics produces transient structures like 'straw' that can explain the halo structure and spectroscopy: Cyclic velocity changes cause perturbed regions to reach higher collision speeds at some orbital phases, which preferentially removes small regolith particles; Surrounding particles diffuse back too slowly to erase the effect: this gives the halo morphology; This requires energetic collisions (v ≈ 10m/sec, with throw distances about 200km, implying objects of scale R ≈ 20km); We propose 'straw'. Transform to Duffing Eqn : With the coordinate transformation, z = M2/3, the Predator-Prey equations can be combined to form a single second-order differential equation with harmonic resonance forcing. Ring dynamics and history implications: Moon

  18. Non-Linear Dynamics of Saturn's Rings

    NASA Astrophysics Data System (ADS)

    Esposito, Larry W.

    2015-04-01

    Non-linear processes can explain why Saturn's rings are so active and dynamic. Ring systems differ from simple linear systems in two significant ways: 1. They are systems of granular material: where particle-to-particle collisions dominate; thus a kinetic, not a fluid description needed. We find that stresses are strikingly inhomogeneous and fluctuations are large compared to equilibrium. 2. They are strongly forced by resonances: which drive a non-linear response, pushing the system across thresholds that lead to persistent states. Some of this non-linearity is captured in a simple Predator-Prey Model: Periodic forcing from the moon causes streamline crowding; This damps the relative velocity, and allows aggregates to grow. About a quarter phase later, the aggregates stir the system to higher relative velocity and the limit cycle repeats each orbit, with relative velocity ranging from nearly zero to a multiple of the orbit average: 2-10x is possible Results of driven N-body systems by Stuart Robbins: Even unforced rings show large variations; Forcing triggers aggregation; Some limit cycles and phase lags seen, but not always as predicted by predator-prey model. Summary of Halo Results: A predator-prey model for ring dynamics produces transient structures like 'straw' that can explain the halo structure and spectroscopy: Cyclic velocity changes cause perturbed regions to reach higher collision speeds at some orbital phases, which preferentially removes small regolith particles; Surrounding particles diffuse back too slowly to erase the effect: this gives the halo morphology; This requires energetic collisions (v ≈ 10m/sec, with throw distances about 200km, implying objects of scale R ≈ 20km); We propose 'straw'. Transform to Duffing Eqn : With the coordinate transformation, z = M2/3, the Predator-Prey equations can be combined to form a single second-order differential equation with harmonic resonance forcing. Ring dynamics and history implications: Moon

  19. New dust belts of Uranus: one ring, two ring, red ring, blue ring.

    PubMed

    de Pater, Imke; Hammel, Heidi B; Gibbard, Seran G; Showalter, Mark R

    2006-04-01

    We compared near-infrared observations of the recently discovered outer rings of Uranus with Hubble Space Telescope results. We find that the inner ring, R/2003 U 2, is red, whereas the outer ring, R/2003 U 1, is very blue. Blue is an unusual color for rings; Saturn's enigmatic E ring is the only other known example. By analogy to the E ring, R/2003 U 1 is probably produced by impacts into the embedded moon Mab, which apparently orbits at a location where nongravitational perturbations favor the survival and spreading of submicron-sized dust. R/2003 U 2 more closely resembles Saturn's G ring, which is red, a typical color for dusty rings. PMID:16601188

  20. Spin-orbit damping in transition metals

    NASA Astrophysics Data System (ADS)

    Gilmore, Keith

    2008-03-01

    Magnetization dynamics are routinely described with the Landau-Lifshitz-Gilbert (LLG) equation. However, it is expected that the LLG equation fails to properly describe the large amplitude dynamics that occur during magnetization reversal. Improving switching speeds in nanoscale devices by tailoring materials requires both a qualitative understanding of the relaxation processes that contribute to damping and the ability to quantitatively calculate the resulting damping rates. We consider small amplitude LLG damping in transition metals as a prelude to approaching the more complicated mechanisms expected in complete reversal events. LLG damping rates in pure transition metal systems have non-monotonic temperature dependencies that have been empirically shown by Heinrich et al. [1] to have one part proportional to the conductivity and one part proportional to the resistivity. Kambersky [2] postulated that both contributions result from a torque between the spin and orbital moments. We have conducted first-principles calculations that validate this claim for single element systems [3]. Our calculations for Fe, Co, and Ni both qualitatively match the two trends observed in measurements and quantitatively agree with the observed damping rates. We will discuss how the spin-orbit interaction produces two contributions to damping with nearly opposite temperature dependencies and compare calculations of the damping rate versus resistivity with experimental results. [1] B. Heinrich, D.J. Meredith, and J.F. Cochran, J. Appl. Phys., 50(11), 7726 (1979). [2] V. Kambersky, Czech. J. Phys. B, 26, 1366 (1976). [3] K. Gilmore, Y.U. Idzerda, and M.D. Stiles, Phys. Rev. Lett., 99, 027204 (2007).

  1. Saturn's F-Ring

    NASA Technical Reports Server (NTRS)

    2000-01-01

    This narrow-angle camera image of Saturn's F Ring was taken through the Clear filter while at a distance of 6.9 million km from Saturn on 8 November 1980. The brightness variations of this tightly-constrained ring shown here indicate that the ring is less uniform in makeup than the larger rings. JPL managed the Voyager Project for NASA's Office of Space Science

  2. Neptune - full ring system

    NASA Technical Reports Server (NTRS)

    1989-01-01

    This pair of Voyager 2 images (FDS 11446.21 and 11448.10), two 591-s exposures obtained through the clear filter of the wide angle camera, show the full ring system with the highest sensitivity. Visible in this figure are the bright, narrow N53 and N63 rings, the diffuse N42 ring, and (faintly) the plateau outside of the N53 ring (with its slight brightening near 57,500 km).

  3. Location and repair of air leaks in the ATF (Advanced Toroidal Facility) vacuum vessel

    SciTech Connect

    Schwenterly, S.W.; Gabbard, W.A.; Schaich, C.R.; Yarber, J.L. )

    1989-01-01

    On the basis of partial pressure rate-of-rise and base pressure measurements, it was determined that the Advanced Toroidal Facility (ATF) vacuum vessel had an air leak in the low 10{sup -4} mbar-{ell}/s range. Pinpointing this leak by conventional helium leak-checking procedures was not possible, because large portions of the outside of the vessel are covered by the helcial field coils and a structural shell. Various alternative leak-detection schemes that were considered are summarized and their advantages and disadvantages noted. In the method ultimately employed, gun-rubber patches of various sizes ranging from 12.7 by 12.7 cm to 20.3 by 30.5 cm were positioned on the inside surfaces of the vessel and evacuated by the leak detector (LD). After roughly 5% of the surface was inspected in this way, a leak of > 10{sup -5} mbar-{ell}/s was discovered and localized to an area of 5 by 5 cm. Dye penetrant applied to this area disclosed three pinholes. Two small slag pockets were discovered while these points were being ground out. After these were rewelded, no furthered leakage could be found in the repaired area. Global leak rates measured after the machine was reevacuated indicated that this leak was about 30% of the overall leak rate. 1 ref., 5 figs., 1 tab.

  4. Analysis of Performance of Selected AFC, ATF Fuels, and Lanthanide Transport

    SciTech Connect

    Unal, Cetin; Galloway, Jack D.

    2015-09-29

    We started to look at the performance of ATF concept in LWRs late in FY14 and finish our studies in FY15. The work has been presented in AFC review meetings, ICAPP and TOPFUEL conferences. The final version of the work is accepted for publication in Nuclear Engineering and Science Journal (NES). The copy of ICAPP and NES papers are attached separately to this document as our milestone deliverables. We made an important progress in the modeling of lanthanide transport in FY15. This work produced an ANS Winter Meeting paper and GLOBAL 2015 paper. GLOBAL 2015 paper is also attached as deliverable of FY15. The work on the lanthanide transport is preliminary. We are exploring other potential mechanisms, in addition to “liquid-like” diffusion mechanisms, proposed by Robert Mariani [1] before we analyze data that will be taken by Ohio State University. This year, we concentrate on developing diffusion kernels and principles of modeling. Next year, this work will continue and analyze the Ohio State data and develop approaches to solve multicomponent diffusion. In addition to three papers we attached to this report, we have done some research on coupling and the development of gas release model for metallic fuels in FY15. They are also preliminary in nature; therefore, we give the summary of what we found rather than an extended report that will be done in FY16.

  5. LASER WAKEFIELD ACCELERATION DRIVEN BY ATF CO2 LASER (STELLA-LW).

    SciTech Connect

    KIMURA,W.D.; ANDREEV,N.E.; BABZIEN,M.; BEN-ZVI,I.; ET AL.

    2004-09-25

    A new experiment has begun that builds upon the successful Staged Electron Laser Acceleration (STELLA) experiment, which demonstrated high-trapping efficiency and narrow energy spread in a staged laser-driven accelerator. STELLA was based upon inverse free electron lasers (IFEL); the new experiment, called STELLA-LW, is based upon laser wakefield acceleration (LWFA). The first phase of STELLA-LW will be to demonstrate LWFA in a capillary discharge driven by the Brookhaven National Laboratory Accelerator Test Facility (ATF) terawatt CO{sub 2} laser beam. This will be the first time LWFA is conducted at 10.6-{micro}m laser wavelength. It will also be operating in an interesting pseudo-resonant regime where the laser pulse length is too long for resonant LWFA, but too short for self-modulated LWFA. Analysis has shown that in pseudo-resonant LWFA, pulse-steepening effects occur on the laser pulse that permits generation of strong wakefields. Various approaches are being explored for the capillary discharge including polypropylene and hydrogen-filled capillaries. Planned diagnostics for the experiment include coherent Thomson scattering (CTS) to detect the wakefield generation. This will be one of the first times CTS is used on a capillary discharge.

  6. Proposal for a study of laser acceleration of electrons using micrograting structures at ATF (Phase 1)

    SciTech Connect

    Chen, W.; Claus, J.; Fernow, R.C.; Fischer, J.; Gallardo, J.C.; Kirk, H.G.; Kramer, H.; Li, Z.; Palmer, R.B.; Rogers, J.; Shrinvasan-Rao, T.; Tsang, T.; Ulc, S.; Veligdan, J.; Warren, J.; Bigio, I.; Kurnit, N.; Shimada, T.; Wang, X.; McDonald, K.T.; Russell, D.P.; Los Alamos National Lab., NM; Princeton Univ., NJ; California Univ., Los Angeles, CA )

    1989-10-29

    We propose to investigate new methods of particle acceleration using a short-pulse CO{sub 2} laser as the power source and grating-like structures as accelerator cavities''. Phase I of this program is intended to demonstrate the principle of the method. We will focus the laser light to a 3 mm line on the surface of the microstructure. The structure is used to transform the electric field pattern of the incoming transversely polarized laser beam to a mode which has a component along the electron beam direction in the vicinity of the surface. With 6 mJ of laser energy and a 6 ps pulse length, the electric field in the spot will be around 1 GV/m. The electron beam from the Brookhaven Accelerator Test Facility (ATF) will be focused transversely within the few micron transverse dimension of the microstructure. The maximum expected acceleration for a 1 GV/m field and a 3 mm acceleration length is 3 MeV. 17 refs., 11 figs., 2 tabs.

  7. High order mode damping in the NSLS accelerating RF cavities by the use of damping antennae

    NASA Astrophysics Data System (ADS)

    Fewell, N.; Wen, Z.

    High order modes were successfully damped in the existing NSLS accelerating cavities by the insertion of damping antennae. The location of the antennae was aided by cavity field plots using superfish and their lengths determined experimentally. A description of their construction is presented together with the results of their insertion upon higher order cavity modes and beam stability.

  8. Collisional damping of the geodesic acoustic mode with toroidal rotation. I. Viscous damping

    NASA Astrophysics Data System (ADS)

    Gong, Xueyu; Xie, Baoyi; Guo, Wenfeng; Chen, You; Yu, Jiangmei; Yu, Jun

    2016-03-01

    With the dispersion relation derived for the geodesic acoustic mode in toroidally rotating tokamak plasmas using the fluid model, the effect of the toroidal rotation on the collisional viscous damping of the geodesic acoustic mode is investigated. It is found that the collisional viscous damping of the geodesic acoustic mode has weak increase with respect to the toroidal Mach number.

  9. Introduction to the scientific application system of DAMPE (On behalf of DAMPE collaboration)

    NASA Astrophysics Data System (ADS)

    Zang, Jingjing

    2016-07-01

    The Dark Matter Particle Explorer (DAMPE) is a high energy particle physics experiment satellite, launched on 17 Dec 2015. The science data processing and payload operation maintenance for DAMPE will be provided by the DAMPE Scientific Application System (SAS) at the Purple Mountain Observatory (PMO) of Chinese Academy of Sciences. SAS is consisted of three subsystems - scientific operation subsystem, science data and user management subsystem and science data processing subsystem. In cooperation with the Ground Support System (Beijing), the scientific operation subsystem is responsible for proposing observation plans, monitoring the health of satellite, generating payload control commands and participating in all activities related to payload operation. Several databases developed by the science data and user management subsystem of DAMPE methodically manage all collected and reconstructed science data, down linked housekeeping data, payload configuration and calibration data. Under the leadership of DAMPE Scientific Committee, this subsystem is also responsible for publication of high level science data and supporting all science activities of the DAMPE collaboration. The science data processing subsystem of DAMPE has already developed a series of physics analysis software to reconstruct basic information about detected cosmic ray particle. This subsystem also maintains the high performance computing system of SAS to processing all down linked science data and automatically monitors the qualities of all produced data. In this talk, we will describe all functionalities of whole DAMPE SAS system and show you main performances of data processing ability.

  10. Necessary and sufficient conditions for the entanglement sudden death under amplitude damping and phase damping

    SciTech Connect

    Huang Jiehui; Zhu Shiyao

    2007-12-15

    By using principal minor method, which is developed from the Peres-Horodecki criterion for the separability of a quantum state, we derive the necessary and sufficient conditions for the entanglement sudden death of a two-qubit state under amplitude damping and phase damping.

  11. Metformin-induced inhibition of the mitochondrial respiratory chain increases FGF21 expression via ATF4 activation

    SciTech Connect

    Kim, Kook Hwan; Jeong, Yeon Taek; Kim, Seong Hun; Jung, Hye Seung; Park, Kyong Soo; Lee, Hae-Youn; Lee, Myung-Shik

    2013-10-11

    Highlights: •Metformin induces FGF21 expression in an AMPK independent manner. •Metformin enhances FGF21 expression by inhibiting mitochondrial complex I activity. •The PERK-eIF2α-ATF4 axis is required for metformin-induced FGF21 expression. •Metformin activates the ATF4-FGF21 axis in the liver of mouse. •Metformin increases serum FGF21 level in diabetic human subjects. -- Abstract: Fibroblast growth factor 21 (FGF21) is an endocrine hormone that exhibits anti-obesity and anti-diabetes effects. Because metformin is widely used as a glucose-lowering agent in patients with type 2 diabetes (T2D), we investigated whether metformin modulates FGF21 expression in cell lines, and in mice or human subjects. We found that metformin increased the expression and release of FGF21 in a diverse set of cell types, including rat hepatoma FaO, primary mouse hepatocytes, and mouse embryonic fibroblasts (MEFs). Intriguingly, AMP-activated protein kinase (AMPK) was dispensable for the induction of FGF21 by metformin. Mammalian target of rapamycin complex 1 (mTORC1) and peroxisome proliferator-activated receptor α (PPARα), which are additional targets of metformin, were not involved in metformin-induced FGF21 expression. Importantly, inhibition of mitochondrial complex I activity by metformin resulted in FGF21 induction through PKR-like ER kinase (PERK)-eukaryotic translation factor 2α (eIF2α)-activating transcription factor 4 (ATF4). We showed that metformin activated ATF4 and increased FGF21 expression in the livers of mice, which led to increased serum levels of FGF21. We also found that serum FGF21 level was increased in human subjects with T2D after metformin therapy for 6 months. In conclusion, our results indicate that metformin induced expression of FGF21 through an ATF4-dependent mechanism by inhibiting mitochondrial respiration independently of AMPK. Therefore, FGF21 induction by metformin might explain a portion of the beneficial metabolic effects of metformin.

  12. PKR Inhibition Rescues Memory Deficit and ATF4 Overexpression in ApoE ε4 Human Replacement Mice.

    PubMed

    Segev, Yifat; Barrera, Iliana; Ounallah-Saad, Hadile; Wibrand, Karin; Sporild, Ida; Livne, Adva; Rosenberg, Tali; David, Orit; Mints, Meshi; Bramham, Clive R; Rosenblum, Kobi

    2015-09-23

    Sporadic Alzheimer's disease (AD) is an incurable neurodegenerative disease with clear pathological hallmarks, brain dysfunction, and unknown etiology. Here, we tested the hypothesis that there is a link between genetic risk factors for AD, cellular metabolic stress, and transcription/translation regulation. In addition, we aimed at reversing the memory impairment observed in a mouse model of sporadic AD. We have previously demonstrated that the most prevalent genetic risk factor for AD, the ApoE4 allele, is correlated with increased phosphorylation of the translation factor eIF2α. In the present study, we tested the possible involvement of additional members of the eIF2α pathway and identified increased mRNA expression of negative transcription factor ATF4 (aka CREB2) both in human and a mouse model expressing the human ApoE4 allele. Furthermore, injection of a PKR inhibitor rescued memory impairment and attenuated ATF4 mRNA increased expression in the ApoE4 mice. The results propose a new mechanism by which ApoE4 affects brain function and further suggest that inhibition of PKR is a way to restore ATF4 overexpression and memory impairment in early stages of sporadic AD. Significance statement: ATF4 mRNA relative quantities are elevated in ApoE4 allele carriers compared with noncarrier controls. This is true also for the ApoE ε4 human replacement mice. ApoE4 mice injected with PKR inhibitor (PKRi) demonstrate a significant reduction in ATF4 expression levels 3 h after one injection of PKRi. Treatment of ApoE4 human replacement mice with the PKRi before learning rescues the memory impairment of the ApoE4 AD model mice. We think that these results propose a new mechanism by which ApoE4 affects brain function and suggest that inhibition of PKR is a way to restore memory impairment in early stages of sporadic AD. PMID:26400930

  13. Saturn's F-Ring

    NASA Technical Reports Server (NTRS)

    2000-01-01

    This narrow-angle camera image of Saturn's F Ring was taken through the Clear filter while at a distance of 0.75 million km from Saturn on 12 November 1980. The kinks and braids of this tightly-constrained ring are visible along with the outer edge of the A Ring. JPL managed the Voyager Project for NASA's Office of Space Science.

  14. The Jumping Ring Experiment

    ERIC Educational Resources Information Center

    Baylie, M.; Ford, P. J.; Mathlin, G. P.; Palmer, C.

    2009-01-01

    The jumping ring experiment has become central to liquid nitrogen shows given as part of the outreach and open day activities carried out within the University of Bath. The basic principles of the experiment are described as well as the effect of changing the geometry of the rings and their metallurgical state. In general, aluminium rings are…

  15. Rings Around Uranus

    ERIC Educational Resources Information Center

    Maran, Stephen P.

    1977-01-01

    Events leading up to the discovery of the rings of Uranus are described. The methods used and the logic behind the methods are explained. Data collected to prove the existence of the rings are outlined and theories concerning the presence of planetary rings are presented. (AJ)

  16. Summary of Working Group on Storage Ring Collective Effects

    SciTech Connect

    Zisman, M.S.

    1987-06-01

    The purposes of this Workshop were to investigate the techniques available for the production of very low emittance electron beams, to explore the limitations of these techniques, and to consider new possibilities that might improve the present situation. Two uses for these low emittance beams are of interest here: to serve for a high energy linear collider, which requires very small beam sizes to achieve a suitable value for the luminosity; and to serve for a free-electron laser (FEL) in the short wavelength - say 40 A - regime, which requires both small transverse beam dimensions and a very low longitudinal emittance. This paper contains a brief summary of the main topics discussed by the Working Group on Storage Ring Collective Effects. In the case of the linear collider application, the use of a damping ring (DR) to reduce, by radiation damping, the emittance of an intermediate energy linac beam prior to its subsequent injection into the remaining high energy linac is considered. For FEL use, a high-gain device with a storage ring to damp the beam periodically between passages through a bypass section containing the long FEL undulator is considered. Such designs - at a longer wavelength of 400 A - are already available, but the shorter wavelength of interest here is much more of a challenge.

  17. Experimental and analytical estimation of damping in beams and plates with damping treatments

    NASA Astrophysics Data System (ADS)

    Liu, Wanbo

    The research presented in this dissertation is devoted to the problem of damping estimation in engineering structures, especially beams and plates with passive damping treatments. In structural design and/or optimization, knowledge about damping is essential. However, due to the complexity of the dynamic interaction of system components, the determination of damping, by either analysis or experiments, has never been straightforward. In this research, currently-used methods are reviewed and gaps are identified first. Then both analytical and experimental studies on the damping estimation are conducted and possibilities of improvement are explored. Various passive damping treatments using ViscoElastic Materials (VEMs) are designed, manufactured and then added to aluminum and composite beams and plates. Experiments on these damped structures are conducted. Currently used experimental methods, namely, the free-decay method, the modal curve-fitting method and the Power Input Method (PIM), are used to process the experimental data and investigate the damping characteristics. Especially, (1) experimental procedures of the power input method are carefully identified and investigated; (2) the power input method is applied to non-uniformly damped structures; (3) the power input method is applied in an extended frequency range (from 0 to 5000 Hz) to meet emerging needs of the transportation industries. A new analytical power input method is proposed for evaluating the loss factor of builtup structures, based on the finite element model with assigned properties of the constituents. Finite Element (FE) models of beams and plates with various damping configurations are developed so a frequency response solution suffices to provide mobility and energy results needed by the new analytical power input method. The analytical power input method is evaluated by comparison with the commonly used Modal Strain Energy (MSE) method. Instead of making an approximate correction of the

  18. Hamiltonian and Lagrangian dynamics of charged particles including the effects of radiation damping

    NASA Astrophysics Data System (ADS)

    Qin, Hong; Burby, Joshua; Davidson, Ronald; Fisch, Nathaniel; Chung, Moses

    2015-11-01

    The effects of radiation damping (radiation reaction) on accelerating charged particles in modern high-intensity accelerators and high-intensity laser beams have becoming increasingly important. Especially for electron accelerators and storage rings, radiation damping is an effective mechanism and technique to achieve high beam luminosity. We develop Hamiltonian and Lagrangian descriptions of the classical dynamics of a charged particle including the effects of radiation damping in the general electromagnetic focusing channels encountered in accelerators. The direct connection between the classical Hamiltonian and Lagrangian theories and the more fundamental QED description of the synchrotron radiation process is also addressed. In addition to their theoretical importance, the classical Hamiltonian and Lagrangian theories of the radiation damping also enable us to numerically integrate the dynamics using advanced structure-preserving geometric algorithms. These theoretical developments can also be applied to runaway electrons and positrons generated during the disruption or startup of tokamak discharges. This research was supported by the U.S. Department of Energy (DE-AC02-09CH11466).

  19. Damping and spectral formation of upstream whistlers

    SciTech Connect

    Orlowski, D.S.; Russell, C.T.; Krauss-Varban, D.

    1995-09-01

    Previous studies have indicated that damping rates of upstream whistlers strongly depend on the details of the electron distribution function. Moreover, detailed analysis of Doppler shift and the whistler dispersion relation indicate that upstream whistlers propagate obliquely in a finite band of frequencies. In this paper we present results of a kinetic calculation of damping lengths of wideband whistlers using the sum of seven drifting bi-Maxwellian electron distributions as a best fit to the ISEE 1 electron data. For two cases, when upstream whistlers are observed, convective damping lengths derived from ISEE magnetic field and ephemeris data are compared with theoretical results. We find that the calculated convective damping lengths are consistent with the data and that upstream whistlers remain marginally stable. We also show that the slope of plasma frame spectra of upstream whistlers, obtained by direct fitting of the observed spectra, is between 5 and 7. The overall spectral, wave, and particle characteristics, proximity to the shock, as well as propagation and damping properties indicated that these waves cannot be generated locally. Instead, the observed upstream whistlers arise in the shock ramp, most likely by a variety of cross-field drift and/or anisotropy driven instabilities. 57 refs., 11 figs.

  20. Noise Transmission Characteristics of Damped Plexiglas Windows

    NASA Technical Reports Server (NTRS)

    Gibbs, Gary P.; Buehrle, Ralph D.; Klos, Jacob; Brown, Sherilyn A.

    2002-01-01

    Most general aviation aircraft utilize single layer plexiglas material for the windshield and side windows. Adding noise control treatments to transparent panels is a challenging problem. In this paper, damped plexiglas windows are evaluated for replacement of conventional windows in general aviation aircraft to reduce the structure-borne and airborne noise transmitted into the interior. In contrast to conventional solid windows, the damped plexiglas window panels are fabricated using two or three layers of plexiglas with transparent viscoelastic damping material sandwiched between the layers. Results from acoustic tests conducted in the NASA Langley Structural Acoustic Loads and Transmission (SALT) facility are used to compare different designs of the damped plexiglas panels with solid windows of the same nominal thickness. Comparisons of the solid and damped plexiglas panels show reductions in the radiated sound power of up to 8 dB at low frequency resonances and as large as 4.5 dB over a 4000 Hz bandwidth. The weight of the viscoelastic treatment was approximately 1% of the panel mass. Preliminary FEM/BEM modeling shows good agreement with experimental results for radiated sound power.

  1. Damped Windows for Aircraft Interior Noise Control

    NASA Technical Reports Server (NTRS)

    Buehrle, Ralph D.; Klos, Jacob; Gibbs, Gary P.

    2004-01-01

    Windows are a significant path for structure-borne and air-borne noise transmission into aircraft. To improve the acoustical performance, damped windows were fabricated using two or three layers of plexiglas with transparent viscoelastic damping material sandwiched between the layers. In this paper, numerical and experimental results are used to evaluate the acoustic benefits of damped windows. Tests were performed in the Structural Acoustic Loads and Transmission Facility at NASA Langley Research Center to measure the transmission loss for diffuse acoustic excitation and radiated sound power for point force excitation. Comparisons between uniform and damped plexiglas windows showed increased transmission loss of 6 dB at the first natural frequency, 6 dB at coincidence, and 4.5 dB over a 50 to 4k Hz range. Radiated sound power was reduced up to 7 dB at the lower natural frequencies and 3.7 dB over a 1000 Hz bandwidth. Numerical models are presented for the prediction of radiated sound power for point force excitation and transmission loss for diffuse acoustic excitation. Radiated sound power and transmission loss predictions are in good agreement with experimental data. A parametric study is presented that evaluates the optimum configuration of the damped plexiglas windows for reducing the radiated sound power.

  2. Jovian Ring System Mosaic

    NASA Technical Reports Server (NTRS)

    2000-01-01

    NASA's Galileo spacecraft acquired this mosaic of Jupiter's ring system (top) when the spacecraft was in Jupiter's shadow looking back toward the Sun. Jupiter's ring system (inset diagram) is composed of three parts: an outermost gossamer ring, a flat main ring, and an innermost donut-shaped halo. These rings are made up of dust-sized particles that are blasted off of the nearby inner satellites by small impacts. This image was taken on November 9, 1996 at a distance of 2.3 million kilometers (1.4 million miles).

  3. Expression and purification of recombinant ATF-mellitin, a new type fusion protein targeting ovarian cancer cells, in P. pastoris.

    PubMed

    Su, Manman; Chang, Weiqin; Zhang, Kun; Cui, Manhua; Wu, Shuying; Xu, Tianmin

    2016-02-01

    Melittin is well known to possess cytolytic activity with wide-spectrum lytic properties and its potential use as an agent to treat several types of cancer has been tested. Due to the non-specific toxicity, melittin can impair not only cancer cells but also normal tissue. Thus, tumor-targeted toxins may be helpful for developing novel anticancer therapeutics. The urokinase-type plasminogen activator (uPA) plays a central role in tissue remodelling events occurring in normal physiology and in pathophysiology, including cancer invasion and metastasis. Heartening findings showed that uPA receptor is predominantly expressed on many types of cancer. Therefore, the amino-terminal fragment (ATF) of uPA which was able to identify and bond with cancer cells was used as the cell-targeting domain to make up tumor-targeted toxin in this study. In the present study, pPICZαC-ATF-melittin eukaryotic expression vector was successfully constructed. After transformed into P. pastoris and induced by methanol, rATF-mellitin was detected by SDS-PAGE and western blot analysis. After induction with methanol, the expression level of rATF-mellitin was 312 mg/l in 80-l fermentor. rATF‑mellitin was purified to >95% purity using SP Sepharose ion exchange chromatography and source™ 30 RPC with 67.2% recovery. Cell proliferation assay showed that rATF-melittin inhibited growth of SKOV3 cells and had no cytotoxicity effect on normal cells. For the first time, we established a stable and effective rATF-mellitin P. pastoris expression system to obtain a high level of expression of secreted rATF-mellitin which was purified by a highly efficient purification procedure. PMID:26718643

  4. CREB, ATF, and AP-1 transcription factors regulate IFN-gamma secretion by human T cells in response to mycobacterial antigen.

    PubMed

    Samten, Buka; Townsend, James C; Weis, Steven E; Bhoumik, Anindita; Klucar, Peter; Shams, Homayoun; Barnes, Peter F

    2008-08-01

    IFN-gamma production by T cells is pivotal for defense against many pathogens, and the proximal promoter of IFN-gamma, -73 to -48 bp upstream of the transcription start site, is essential for its expression. However, transcriptional regulation mechanisms through this promoter in primary human cells remain unclear. We studied the effects of cAMP response element binding protein/activating transcription factor (CREB/ATF) and AP-1 transcription factors on the proximal promoter of IFN-gamma in human T cells stimulated with Mycobacterium tuberculosis. Using EMSA, supershift assays, and promoter pulldown assays, we demonstrated that CREB, ATF-2, and c-Jun, but not cyclic AMP response element modulator, ATF-1, or c-Fos, bind to the proximal promoter of IFN-gamma upon stimulation, and coimmunoprecipitation indicated the possibility of interaction among these transcription factors. Chromatin immunoprecipitation confirmed the recruitment of these transcription factors to the IFN-gamma proximal promoter in live Ag-activated T cells. Inhibition of ATF-2 activity in T cells with a dominant-negative ATF-2 peptide or with small interfering RNA markedly reduced the expression of IFN-gamma and decreased the expression of CREB and c-Jun. These findings suggest that CREB, ATF-2, and c-Jun are recruited to the IFN-gamma proximal promoter and that they up-regulate IFN-gamma transcription in response to microbial Ag. Additionally, ATF-2 controls expression of CREB and c-Jun during T cell activation. PMID:18641343

  5. Differential ATF3 expression in dorsal root ganglion neurons reveals the profile of primary afferents engaged by diverse noxious chemical stimuli

    PubMed Central

    Bráz, João M.; Basbaum, Allan I.

    2010-01-01

    Although transgenic and knockout mice have helped delineate the mechanisms of action of diverse noxious compounds, it is still difficult to determine unequivocally the subpopulations of primary afferent nociceptor that these molecules engage. As most noxious stimuli lead to tissue and/or nerve injury, here we used induction of activating transcription factor 3 (ATF3), a reliable marker of nerve injury, to assess the populations of primary afferent fibers that are activated after peripheral administration of noxious chemical stimuli. In wild-type mice, hindpaw injections of capsaicin, formalin, mustard oil or menthol induce expression of ATF3 in distinct subpopulations of sensory neurons. Interestingly, even though these noxious chemicals are thought to act through subtypes of transient receptor potential (TRP) channels, all compounds also induced ATF3 in neurons that appear not to express the expected TRP channel subtypes. On the other hand, capsaicin failed to induce ATF3 in mice lacking TRPV1, indicating that TRPV1 is required for both the direct and indirect induction of ATF3 in sensory neurons. By contrast, only low doses of formalin or mustard oil failed to induce ATF3 in TRPA1 null mice, indicating that injections of high doses (>0.5%) of formalin or mustard oil recruit both TRPA1 and non-TRPA1 expressing primary afferent fibers. Finally, peripheral injection of menthol, a TRPM8 receptor agonist, induced ATF3 in a wide variety of sensory neurons, but in a TRPM8-independent manner. We conclude that purportedly selective agonists can activate a heterogeneous population of sensory neurons, which ultimately could contribute to the behavioral responses evoked. PMID:20605331

  6. Passive damping concepts for slender columns in space structures

    NASA Technical Reports Server (NTRS)

    Razzaq, Z.; Ekhelikar, R. K.

    1985-01-01

    An experimental and theoretical study of three different passive damping concepts is conducted for a slender member with partial rotational end restraints. Over a hundred full-scale natural vibration experiments were conducted to evaluate the effectiveness of mass-string, polyethylene tubing, and chain damping concepts. The damping properties obtained from the experiments were used in the approximate analyses based on the partial differential equation of motion for the problem. The comparison of the experimental and the theoretical deflection-time relations shows that the velocity-dependent damping model used in the theory is adequate. From the experimental results, the effect of end connection friction and induced axial forces on damping is identified. The definition of an efficiency index is proposed based on the damping ratio and the mass of a given passive damping device. Using this definition, the efficiencies of the three damping devices are compared. The polyethylene tubing concept resulted into a low damping efficiency.

  7. Saturn's largest ring.

    PubMed

    Verbiscer, Anne J; Skrutskie, Michael F; Hamilton, Douglas P

    2009-10-22

    Most planetary rings in the Solar System lie within a few radii of their host body, because at these distances gravitational accelerations inhibit satellite formation. The best known exceptions are Jupiter's gossamer rings and Saturn's E ring, broad sheets of dust that extend outward until they fade from view at five to ten planetary radii. Source satellites continuously supply the dust, which is subsequently lost in collisions or by radial transport. Here we report that Saturn has an enormous ring associated with its outer moon Phoebe, extending from at least 128R(S) to 207R(S) (Saturn's radius R(S) is 60,330 km). The ring's vertical thickness of 40R(S) matches the range of vertical motion of Phoebe along its orbit. Dynamical considerations argue that these ring particles span the Saturnian system from the main rings to the edges of interplanetary space. The ring's normal optical depth of approximately 2 x 10(-8) is comparable to that of Jupiter's faintest gossamer ring, although its particle number density is several hundred times smaller. Repeated impacts on Phoebe, from both interplanetary and circumplanetary particle populations, probably keep the ring populated with material. Ring particles smaller than centimetres in size slowly migrate inward and many of them ultimately strike the dark leading face of Iapetus. PMID:19812546

  8. Radiative Damping and Functional Differential Equations

    NASA Astrophysics Data System (ADS)

    Raju, Suvrat; Raju, C. K.

    We propose a general technique to solve the classical many-body problem with radiative damping. We modify the short-distance structure of Maxwell electrodynamics. This allows us to avoid runaway solutions as if we had a covariant model of extended particles. The resulting equations of motion are functional differential equations (FDEs) rather than ordinary differential equations (ODEs). Using recently developed numerical techniques for stiff, retarded FDEs, we solve these equations for the one-body central force problem with radiative damping. Our results indicate that locally the magnitude of radiation damping may be well approximated by the standard third-order expression but the global properties of our solutions are dramatically different. We comment on the two-body problem and applications to quantum field theory and quantum mechanics.

  9. Resolving photons from cosmic ray in DAMPE

    NASA Astrophysics Data System (ADS)

    Xu, Zunlei; Chang, Jin; Li, Xiang; Dong, TieKuang; Zang, Jingjing

    2016-07-01

    The Dark Matter Particle Explorer(DAMPE), which took to the skies on 17 December, is designed for high energy cosmic ray ion detection. The proportion of photons in the cosmic ray is very small, so it's difficult to distinguish between photons and 'background', but necessary for any DAMPE gamma-ray science goals.The paper present a algorithm to identify photons from 'background' mainly by the tracker/converter, which promote pair conversion and measure the directions of incident particles, and an anticoincidence detector,featuring an array of plastic scintillator to detect the charged particles.The method has been studied by simulating using the GEANT4 Monte Carlo simulation code and adjusted by the BeamTest at CERN in December,2014.In addition,DAMPE photon detection capabilities can be checked using the flight data.

  10. Highly damped kinematic coupling for precision instruments

    DOEpatents

    Hale, Layton C.; Jensen, Steven A.

    2001-01-01

    A highly damped kinematic coupling for precision instruments. The kinematic coupling provides support while causing essentially no influence to its nature shape, with such influences coming, for example, from manufacturing tolerances, temperature changes, or ground motion. The coupling uses three ball-cone constraints, each combined with a released flexural degree of freedom. This arrangement enables a gain of higher load capacity and stiffness, but can also significantly reduce the friction level in proportion to the ball radius divided by the distance between the ball and the hinge axis. The blade flexures reduces somewhat the stiffness of the coupling and provides an ideal location to apply constrained-layer damping which is accomplished by attaching a viscoelastic layer and a constraining layer on opposite sides of each of the blade flexures. The three identical ball-cone flexures provide a damped coupling mechanism to kinematically support the projection optics system of the extreme ultraviolet lithography (EUVL) system, or other load-sensitive apparatus.

  11. Passively damped vibration welding system and method

    DOEpatents

    Tan, Chin-An; Kang, Bongsu; Cai, Wayne W.; Wu, Tao

    2013-04-02

    A vibration welding system includes a controller, welding horn, an anvil, and a passive damping mechanism (PDM). The controller generates an input signal having a calibrated frequency. The horn vibrates in a desirable first direction at the calibrated frequency in response to the input signal to form a weld in a work piece. The PDM is positioned with respect to the system, and substantially damps or attenuates vibration in an undesirable second direction. A method includes connecting the PDM having calibrated properties and a natural frequency to an anvil of an ultrasonic welding system. Then, an input signal is generated using a weld controller. The method includes vibrating a welding horn in a desirable direction in response to the input signal, and passively damping vibration in an undesirable direction using the PDM.

  12. Turbine blade damping device with controlled loading

    SciTech Connect

    Marra, John J.

    2015-09-29

    A damping structure for a turbomachine rotor. The damping structure including an elongated snubber element including a first snubber end rigidly attached to a first blade and extending toward an adjacent second blade, and an opposite second snubber end positioned adjacent to a cooperating surface associated with the second blade. The snubber element has a centerline extending radially inwardly in a direction from the first blade toward the second blade along at least a portion of the snubber element between the first and second snubber ends. Rotational movement of the rotor effects relative movement between the second snubber end and the cooperating surface to position the second snubber end in frictional engagement with the cooperating surface with a predetermined damping force determined by a centrifugal force on the snubber element.

  13. Fluid damping of cylindrical liquid storage tanks.

    PubMed

    Habenberger, Joerg

    2015-01-01

    A method is proposed in order to calculate the damping effects of viscous fluids in liquid storage tanks subjected to earthquakes. The potential equation of an ideal fluid can satisfy only the boundary conditions normal to the surface of the liquid. To satisfy also the tangential interaction conditions between liquid and tank wall and tank bottom, the potential flow is superimposed by a one-dimensional shear flow. The shear flow in this boundary layer yields to a decrease of the mechanical energy of the shell-liquid-system. A damping factor is derived from the mean value of the energy dissipation in time. Depending on shell geometry and fluid viscosity, modal damping ratios are calculated for the convective component. PMID:26405635

  14. Saturation of gyrokinetic turbulence through damped eigenmodes.

    PubMed

    Hatch, D R; Terry, P W; Jenko, F; Merz, F; Nevins, W M

    2011-03-18

    In the context of toroidal gyrokinetic simulations, it is shown that a hierarchy of damped modes is excited in the nonlinear turbulent state. These modes exist at the same spatial scales as the unstable eigenmodes that drive the turbulence. The larger amplitude subdominant modes are weakly damped and exhibit smooth, large-scale structure in velocity space and in the direction parallel to the magnetic field. Modes with increasingly fine-scale structure are excited to decreasing amplitudes. In aggregate, damped modes define a potent energy sink. This leads to an overlap of the spatial scales of energy injection and peak dissipation, a feature that is in contrast with more traditional turbulent systems. PMID:21469869

  15. Viscous damping of perforated planar micromechanical structures

    PubMed Central

    Homentcovschi, D.; Miles, R.N.

    2008-01-01

    The paper gives an analytical approximation to the viscous damping coefficient due to the motion of a gas between a pair of closely spaced fluctuating plates in which one of the plates contains a regular system of circular holes. These types of structures are important parts of many microelectromechanical devices realized in MEMS technology as microphones, microaccelerometers, resonators, etc. The pressure satisfies a Reynolds’ type equation with coefficients accounting for all the important effects: compressibility of the gas, inertia and possibly slip of the gas on the plates. An analytical expression for the optimum number of circular holes which assure a minimum value of the total damping coefficient is given. This value realizes an equilibrium between the squeeze-film damping and the viscous resistance of the holes. The paper also provides analytical design formulas to be used in the case of regular circular perforated plates. PMID:19365579

  16. Biomimetic Gradient Polymers with Enhanced Damping Capacities.

    PubMed

    Wang, Dong; Zhang, Huan; Guo, Jing; Cheng, Beichen; Cao, Yuan; Lu, Shengjun; Zhao, Ning; Xu, Jian

    2016-04-01

    Designing gradient structures, mimicking biological materials, such as pummelo peels and tendon, is a promising strategy for developing advanced materials with superior energy damping capacities. Here a facile and effective approach for fabricating polymers with composition gradients at millimeter length scale is presented. The gradient thiol-ene polymers (TEPs) are created by the use of density difference of ternary thiol-ene-ene precursors and the subsequent photo-crosslinking via thiol-ene reaction. The compositional gradients are analyzed via differential scanning calorimeter (DSC), compressive modulus testing, atomic force microscopy (AFM) indentation, and swelling measurements. In contrast to homogeneous TEPs networks, the resultant gradient polymer shows a broader effective damping temperature range combining with good mechanical properties. The present result provides an effective route toward high damping materials by the fabrication of gradient structures. PMID:26776353

  17. Turbine blade damping device with controlled loading

    SciTech Connect

    Marra, John J

    2013-09-24

    A damping structure for a turbomachine rotor. The damping structure including an elongated snubber element including a first snubber end rigidly attached to a first blade and extending toward an adjacent second blade, and an opposite second snubber end positioned adjacent to a cooperating surface associated with the second blade. The snubber element has a centerline extending radially inwardly in a direction from the first blade toward the second blade along at least a portion of the snubber element between the first and second snubber ends. Rotational movement of the rotor effects relative movement between the second snubber end and the cooperating surface to position the second snubber end in frictional engagement with the cooperating surface with a predetermined damping force determined by a centrifugal force on the snubber element.

  18. Measurement of damping of graphite epoxy materials

    NASA Technical Reports Server (NTRS)

    Crocker, M. J.

    1985-01-01

    The design of an experiment to measure the damping of a cylindrical graphite-epoxy specimen with a three point support and a knife edge support is described as well as equipment used in tests conducted to determine the influence of the support at the two ends of the specimen and to simulate an idealized free-free boundary condition at the two edges. A curve fitting technique is being used to process the frequency response data obtained. Experiments conducted on the thin plate specimen also reveal the influence of the end support condition on the damping ratio of the specimen. The damping ratio values measured for both specimens appear to be strongly influenced by the shape of the specimen and appear to depend on length and fiber orientation as well as the presence of discontinuities such as sharp bends, corners, and notches.

  19. Identification of civil structures with nonproportional damping

    NASA Astrophysics Data System (ADS)

    Yang, Jann N.; Lei, Ying

    2000-04-01

    Recently, the method of Hilbert transform has been used successfully by the authors to identify parameters of linear structures with real eigenvalues and eigenvectors, e.g., structures with proportional damping. Frequently, linear structures may not have proportional damping so that normal modes do not exist. In this case, all the eigenvalues, eigenvectors and modeshapes are complex. In this paper, the Hilbert transform and the method of Empirical Mode Decomposition are used to identify the parameters of structures with nonproportional damping using the impulse response data. Measured impulse response signals are first decomposed into Intrinsic Mode Functions using the method of Empirical Mode Decomposition with intermittency criteria. An Intrinsic Mode Function (IMF) contains only one characteristic time scale (frequency), which may involve the contribution of a complex conjugate pair of modes with a unique frequency and a damping ratio, referred to as the modal response. It is shown that all the modal responses can be obtained from IMFs. Then, each modal response is decomposed in the frequency-time domain to yield instantaneous phase angle and amplitude as functions of time using the Hilbert transform. Based on only a single measurement of the impulse response time history at one location, the complex eigenvalues of the linear structure can be identified using a simple analysis procedure. When the response time histories are measured at all locations, the proposed methodology is capable of identifying the complex modeshapes as well as the mass, damping and stiffness matrices of the structure. The effectiveness and accuracy of the methodology presented are demonstrated through numerical simulations. It is shown that complete dynamic characteristics of linear structures with nonproportional damping can be identified effectively using the Hilbert transform and the Empirical Mode Decomposition method.

  20. Damping Higher Order Modes in the PEP-II B-Factory Vertex Bellows

    SciTech Connect

    Weathersby, S.; Langton, J.; Novokhatski, A.; Seeman, J.; /SLAC

    2005-06-30

    Higher stored currents and shorter bunch lengths are requirements for increasing luminosity in colliding storage rings. As a result, more HOM power is generated in the IP region. This HOM power propagates to sensitive components causing undesirable heating, thus becoming a limiting issue for the PEP-II B-factory. HOM field penetration through RF shielding fingers has been shown to cause heating in bellows structures. To overcome these limitations, a proposal to incorporate ceramic absorbers within the bellows cavity to damp these modes is presented. Results show that the majority of modes of interest are damped, the effectiveness depending on geometrical considerations. An optimal configuration is presented for the PEP-II B-factory IR bellows component utilizing commercial grade ceramics with consideration for heat transfer requirements.

  1. Particle systems and nonlinear Landau damping

    SciTech Connect

    Villani, Cédric

    2014-03-15

    Some works dealing with the long-time behavior of interacting particle systems are reviewed and put into perspective, with focus on the classical Kolmogorov–Arnold–Moser theory and recent results of Landau damping in the nonlinear perturbative regime, obtained in collaboration with Clément Mouhot. Analogies are discussed, as well as new qualitative insights in the theory. Finally, the connection with a more recent work on the inviscid Landau damping near the Couette shear flow, by Bedrossian and Masmoudi, is briefly discussed.

  2. Inertia-Wheel Vibration-Damping System

    NASA Technical Reports Server (NTRS)

    Fedor, Joseph V.

    1990-01-01

    Proposed electromechanical system would damp vibrations in large, flexible structure. In active vibration-damping system motors and reaction wheels at tips of appendages apply reaction torques in response to signals from accelerometers. Velocity signal for vibrations about one axis processes into control signal to oppose each of n vibrational modes. Various modes suppressed one at a time. Intended primarily for use in spacecraft that has large, flexible solar panels and science-instrument truss assembly, embodies principle of control interesting in its own right and adaptable to terrestrial structures, vehicles, and instrument platforms.

  3. Delay of Transition Using Forced Damping

    NASA Technical Reports Server (NTRS)

    Exton, Reginald J.

    2014-01-01

    Several experiments which have reported a delay of transition are analyzed in terms of the frequencies of the induced disturbances generated by different flow control elements. Two of the experiments employed passive stabilizers in the boundary layer, one leading-edge bluntness, and one employed an active spark discharge in the boundary layer. It is found that the frequencies generated by the various elements lie in the damping region of the associated stability curve. It is concluded that the creation of strong disturbances in the damping region stabilizes the boundary-layer and delays the transition from laminar to turbulent flow.

  4. Damping of Bogoliubov excitations at finite temperatures

    NASA Astrophysics Data System (ADS)

    Pastukhov, Volodymyr

    2015-10-01

    We present a simple and efficient method to calculate the damping for the excitation spectrum of a uniform D-dimensional Bose gas. Starting from the original Popov’s hydrodynamic description and integrating out phase variables, we obtained the effective action of amplitude fluctuations. Within this approach, the lifetime of quasi-particles with a finite momentum is calculated at a wide temperature range. It is shown that the correct use of the hydrodynamic approach leads to the damping rate, which coincides with results obtained by means of the perturbation theory.

  5. Minimal energy damping in an axisymmetric flow

    NASA Astrophysics Data System (ADS)

    Sachs, Alexander

    2008-05-01

    The method of Lagrange's undetermined multipliers is used to find the velocity field which minimizes the energy damping for a viscous incompressible fluid described by the Navier- Stoke equation. The vorticity of this velocity field obeys a Helmholtz equation with an undetermined parameter. This Helmholtz equation is used to determine the axisymmetric velocity field in a cylinder. This velocity field is slightly different from the Poiseuille velocity field. The rate of energy damping per unit energy is calculated as a function of the parameter. It is a minimum when the parameter is equal to the root of a Bessel function.

  6. Damping Goes the Distance in Golf

    NASA Technical Reports Server (NTRS)

    2004-01-01

    In the late 1980s, Dr. Benjamin Dolgin of NASA s Jet Propulsion Laboratory developed a concept for a high-damping graphite/viscoelastic material for the Strategic Defense Initiative (popularly referred to as "Star Wars"), as part of a space-based laser anti-missile program called "Asterix." Dolgin drummed up this concept with the intention of stabilizing weapons launch platforms in space, where there is no solid ground to firmly support these structures. Without the inclusion of high-damping material, the orbital platforms were said to vibrate for 20 minutes after force was applied - a rate deemed "unacceptable" by leaders of the Strategic Defense Initiative.

  7. Impact of Saturn Main Ring Mass on interpretation of Pioneer 11 and Cassini SOI Radiation Measurements Across the Rings

    NASA Astrophysics Data System (ADS)

    Cooper, John F.; Sturner, Steven J.; Sittler, Edward C., Jr.; Kollmann, Peter; Roussos, Elias; Johnson, Robert E.

    2015-11-01

    The Pioneer 11 (1979) and Cassini Orbiter (2004) missions measured the energetic particle and gamma ray flux environments across the A, B, and outer C rings of Saturn. This radiation originates as secondary proton, neutron, electron, and gamma ray emissions from the interaction of high-energy (> 20 GeV) galactic cosmic ray protons and other ions with bulk ice material in the rings and is sensitive to the surface mass density of the rings. The Pioneer 11 analysis from the University of Chicago High Energy Telescope, published in 1985, was consistent with a average surface density of about 50 g/cm2, assuming pure water ice, and a total ring mass of 2.7x10-8 Saturn masses (MS). This independently-derived value confirmed the post-Voyager result of 3x10-8 MS from radio and stellar occultations, and from observed damping of density waves in the rings. Although some later ring models in the Cassini mission era (2004 - present) allow for a greater mass by an order of magnitude, the latest density wave analysis from Cassini indicates that the Pioneer-Voyager value may be correct. GEANT radiation transport simulations have been performed to update the ring radiation model and enable ongoing assessments of the Pioneer 11 HET and Cassini MIMI/LEMMS responses to this radiation. The O2 gas production by radiation chemistry within the ring material is also estimated as a function of ring mass for comparison to Cassini and earlier measurements of the ring atmosphere and ionosphere. More massive rings would produce more O2.

  8. Assessing Equivalent Viscous Damping Using Piping System test Results

    SciTech Connect

    Nie, J.; Morante, R.

    2010-07-18

    The specification of damping for nuclear piping systems subject to seismic-induced motions has been the subject of many studies and much controversy. Damping estimation based on test data can be influenced by numerous factors, consequently leading to considerable scatter in damping estimates in the literature. At present, nuclear industry recommendations and nuclear regulatory guidance are not consistent on the treatment of damping for analysis of nuclear piping systems. Therefore, there is still a need to develop a more complete and consistent technical basis for specification of appropriate damping values for use in design and analysis. This paper summarizes the results of recent damping studies conducted at Brookhaven National Laboratory.

  9. Dust and Planetary Rings

    NASA Astrophysics Data System (ADS)

    Siddiqui, Muddassir

    ABSTRACT Space is not empty it has comic radiations (CMBR), dust etc. Cosmic dust is that type of dust which is composed of particles in space which vary from few molecules to 0.1micro metres in size. This type of dust is made up of heavier atoms born in the heart of stars and supernova. Mainly it contains dust grains and when these dust grains starts compacting then it turns to dense clouds, planetary ring dust and circumstellar dust. Dust grains are mainly silicate particles. Dust plays a major role in our solar system, for example in zodiacal light, Saturn's B ring spokes, planetary rings at Jovian planets and comets. Observations and measurements of cosmic dust in different regions of universe provide an important insight into the Universe's recycling processes. Astronomers consider dust in its most recycled state. Cosmic dust have radiative properties by which they can be detected. Cosmic dusts are classified as intergalactic dusts, interstellar dusts and planetary rings. A planetary ring is a ring of cosmic dust and other small particles orbiting around a planet in flat disc shape. All of the Jovian planets in our solar system have rings. But the most notable one is the Saturn's ring which is the brightest one. In March 2008 a report suggested that the Saturn's moon Rhea may have its own tenuous ring system. The ring swirling around Saturn consists of chunks of ice and dust. Most rings were thought to be unstable and to dissipate over course of tens or hundreds of millions of years but it now appears that Saturn's rings might be older than that. The dust particles in the ring collide with each other and are subjected to forces other than gravity of its own planet. Such collisions and extra forces tend to spread out the rings. Pluto is not known to have any ring system but some Astronomers believe that New Horizons probe might find a ring system when it visits in 2015.It is also predicted that Phobos, a moon of Mars will break up and form into a planetary ring

  10. Traceable Ring Signature

    NASA Astrophysics Data System (ADS)

    Fujisaki, Eiichiro; Suzuki, Koutarou

    The ring signature allows a signer to leak secrets anonymously, without the risk of identity escrow. At the same time, the ring signature provides great flexibility: No group manager, no special setup, and the dynamics of group choice. The ring signature is, however, vulnerable to malicious or irresponsible signers in some applications, because of its anonymity. In this paper, we propose a traceable ring signature scheme. A traceable ring scheme is a ring signature except that it can restrict “excessive” anonymity. The traceable ring signature has a tag that consists of a list of ring members and an issue that refers to, for instance, a social affair or an election. A ring member can make any signed but anonymous opinion regarding the issue, but only once (per tag). If the member submits another signed opinion, possibly pretending to be another person who supports the first opinion, the identity of the member is immediately revealed. If the member submits the same opinion, for instance, voting “yes” regarding the same issue twice, everyone can see that these two are linked. The traceable ring signature can suit to many applications, such as an anonymous voting on a BBS. We formalize the security definitions for this primitive and show an efficient and simple construction in the random oracle model.

  11. Sunset on Saturn's Rings

    NASA Technical Reports Server (NTRS)

    1996-01-01

    This is a rare view of Saturn's rings seen just after the Sun has set below the ring plane, taken with the Hubble Space Telescope on Nov. 21, 1995.

    This perspective is unusual because the Earth is slightly above (2.7 degrees latitude) Saturn's rings and the Sun is below them. Normally we see the rings fully illuminated by the Sun.

    The photograph shows three bright ring features: the F Ring, the Cassini Division, and the C Ring (moving from the outer rings to the inner). The low concentration of material in these rings allows light from the Sun to shine through them. The A and B rings are much denser, which limits the amount of light that penetrates through them. Instead, they are faintly visible because they reflect light from Saturn's disk.

    Scientists believe that the F Ring is slightly warped because it disappears part way around on the right (West) side. Hubble's high resolution shows the that A Ring's shadow obscures part of the F ring (right).

    The image was assembled from 20 exposures taken with Wide Field Planetary Camera-2 over 8 hours.

    The Wide Field/Planetary Camera 2 was developed by the Jet Propulsion Laboratory and managed by the Goddard Spaced Flight Center for NASA's Office of Space Science.

    This image and other images and data received from the Hubble Space Telescope are posted on the World Wide Web on the Space Telescope Science Institute home page at URL http://oposite.stsci.edu/pubinfo/

  12. Regulation of the death-associated protein kinase 1 expression and autophagy via ATF6 requires apoptosis signal-regulating kinase 1.

    PubMed

    Gade, Padmaja; Manjegowda, Srikanta B; Nallar, Shreeram C; Maachani, Uday B; Cross, Alan S; Kalvakolanu, Dhananjaya V

    2014-11-01

    The death-associated protein kinase 1 (DAPK1) is an important regulator of cell death and autophagy. Recently, we have identified that ATF6, an endoplasmic reticulum-resident transcription factor, in association with the transcription factor CEBP-β, regulates the gamma interferon (IFN-γ)-induced expression of Dapk1 (P. Gade et al., Proc. Natl. Acad. Sci. U. S. A. 109:10316-10321, 2012, doi.org/10.1073/pnas.1119273109). IFN-γ-induced proteolytic processing of ATF6 and phosphorylation of C/EBP-β were essential for the formation of a novel transcriptional complex that regulates DAPK1. Here, we report that IFN-γ activates the ASK1-MKK3/MKK6-p38 mitogen-activated protein kinase (MAPK) pathway for controlling the activity of ATF6. The terminal enzyme in this pathway, p38 MAPK, phosphorylates a critical threonine residue in ATF6 upstream of its DNA binding domain. ATF6 mutants defective for p38 MAPK phosphorylation fail to undergo proteolytic processing in the Golgi apparatus and drive IFN-γ-induced gene expression and autophagy. We also show that mice lacking Ask1 are highly susceptible to lethal bacterial infection owing to defective autophagy. Together, these results identify a novel host defense pathway controlled by IFN-γ signaling. PMID:25135476

  13. Boric acid induces cytoplasmic stress granule formation, eIF2α phosphorylation, and ATF4 in prostate DU-145 cells.

    PubMed

    Henderson, Kimberly A; Kobylewski, Sarah E; Yamada, Kristin E; Eckhert, Curtis D

    2015-02-01

    Dietary boron intake is associated with reduced prostate and lung cancer risk and increased bone mass. Boron is absorbed and circulated as boric acid (BA) and at physiological concentrations is a reversible competitive inhibitor of cyclic ADP ribose, the endogenous agonist of the ryanodine receptor calcium (Ca(+2)) channel, and lowers endoplasmic reticulum (ER) [Ca(2+)]. Low ER [Ca(2+)] has been reported to induce ER stress and activate the eIF2α/ATF4 pathway. Here we report that treatment of DU-145 prostate cells with physiological levels of BA induces ER stress with the formation of stress granules and mild activation of eIF2α, GRP78/BiP, and ATF4. Mild activation of eIF2α and its downstream transcription factor, ATF4, enables cells to reconfigure gene expression to manage stress conditions and mild activation of ATF4 is also required for the differentiation of osteoblast cells. Our results using physiological levels of boric acid identify the eIF2α/ATF pathway as a plausible mode of action that underpins the reported health effects of dietary boron. PMID:25425213

  14. Endoplasmic Reticulum Stress-responsive Transcription Factor ATF6α Directs Recruitment of the Mediator of RNA Polymerase II Transcription and Multiple Histone Acetyltransferase Complexes*♦

    PubMed Central

    Sela, Dotan; Chen, Lu; Martin-Brown, Skylar; Washburn, Michael P.; Florens, Laurence; Conaway, Joan Weliky; Conaway, Ronald C.

    2012-01-01

    The basic leucine zipper transcription factor ATF6α functions as a master regulator of endoplasmic reticulum (ER) stress response genes. Previous studies have established that, in response to ER stress, ATF6α translocates to the nucleus and activates transcription of ER stress response genes upon binding sequence specifically to ER stress response enhancer elements in their promoters. In this study, we investigate the biochemical mechanism by which ATF6α activates transcription. By exploiting a combination of biochemical and multidimensional protein identification technology-based mass spectrometry approaches, we have obtained evidence that ATF6α functions at least in part by recruiting to the ER stress response enhancer elements of ER stress response genes a collection of RNA polymerase II coregulatory complexes, including the Mediator and multiple histone acetyltransferase complexes, among which are the Spt-Ada-Gcn5 acetyltransferase (SAGA) and Ada-Two-A-containing (ATAC) complexes. Our findings shed new light on the mechanism of action of ATF6α, and they outline a straightforward strategy for applying multidimensional protein identification technology mass spectrometry to determine which RNA polymerase II transcription factors and coregulators are recruited to promoters and other regulatory elements to control transcription. PMID:22577136

  15. Damping of the wrist joint during voluntary movement.

    PubMed

    Milner, T E; Cloutier, C

    1998-10-01

    Damping characteristics of the musculoskeletal system were investigated during rapid voluntary wrist flexion movements. Oscillations about the final position were induced by introducing a load with the characteristics of negative damping, which artificially reduced the damping of the wrist. Subjects responded to increases in the negatively damped load by stronger cocontraction of wrist flexor and extensor muscles during the stabilization phase of the movement. However, their ability to counteract the effects of the negatively damped load diminished as the negative damping increased. Consequently, the number and frequency of oscillations increased. The oscillations were accompanied by phase-locked muscle activity superimposed on underlying tonic muscle activation. The wrist stiffness and damping coefficient increased with the increased cocontraction that accompanied more negatively damped loads, although changes in the damping coefficient were less systematic than the stiffness. Analysis of successive half-cycles of the oscillation revealed that the wrist stiffness and damping coefficient increased, despite decreasing muscle activation, as oscillation amplitude and velocity declined. This indicates that the inverse dependence of the damping coefficient on oscillation velocity contributes significantly to damping of joint motion. It is suggested that this property helps to offset a negative contribution to damping from the stretch reflex. PMID:9808304

  16. Non-Linear Dynamics of Saturn's Rings

    NASA Astrophysics Data System (ADS)

    Esposito, L. W.

    2015-12-01

    Non-linear processes can explain why Saturn's rings are so active and dynamic. Some of this non-linearity is captured in a simple Predator-Prey Model: Periodic forcing from the moon causes streamline crowding; This damps the relative velocity, and allows aggregates to grow. About a quarter phase later, the aggregates stir the system to higher relative velocity and the limit cycle repeats each orbit, with relative velocity ranging from nearly zero to a multiple of the orbit average: 2-10x is possible. Summary of Halo Results: A predator-prey model for ring dynamics produces transient structures like 'straw' that can explain the halo structure and spectroscopy: Cyclic velocity changes cause perturbed regions to reach higher collision speeds at some orbital phases, which preferentially removes small regolith particles; Surrounding particles diffuse back too slowly to erase the effect: this gives the halo morphology; This requires energetic collisions (v ≈ 10m/sec, with throw distances about 200km, implying objects of scale R ≈ 20km); We propose 'straw', as observed ny Cassini cameras. Transform to Duffing Eqn : With the coordinate transformation, z = M2/3, the Predator-Prey equations can be combined to form a single second-order differential equation with harmonic resonance forcing. Ring dynamics and history implications: Moon-triggered clumping at perturbed regions in Saturn's rings creates both high velocity dispersion and large aggregates at these distances, explaining both small and large particles observed there. This confirms the triple architecture of ring particles: a broad size distribution of particles; these aggregate into temporary rubble piles; coated by a regolith of dust. We calculate the stationary size distribution using a cell-to-cell mapping procedure that converts the phase-plane trajectories to a Markov chain. Approximating the Markov chain as an asymmetric random walk with reflecting boundaries allows us to determine the power law index from

  17. Landau damping of Gardner solitons in a dusty bi-ion plasma

    NASA Astrophysics Data System (ADS)

    Misra, A. P.; Barman, Arnab

    2015-07-01

    The effects of linear Landau damping on the nonlinear propagation of dust-acoustic solitary waves (DASWs) are studied in a collisionless unmagnetized dusty plasma with two species of positive ions. The extremely massive, micron-seized, cold, and negatively charged dust particles are described by fluid equations, whereas the two species of positive ions, namely, the cold (heavy) and hot (light) ions are described by the kinetic Vlasov equations. Following Ott and Sudan [Phys. Fluids 12, 2388 (1969)], and by considering lower and higher-order perturbations, the evolution of DASWs with Landau damping is shown to be governed by Korteweg-de Vries (KdV), modified KdV (mKdV), or Gardner (KdV-mKdV)-like equations. The properties of the phase velocity and the Landau damping rate of DASWs are studied for different values of the ratios of the temperatures (σ) and the number densities (μ) of hot and cold ions as well as the cold to hot ion mass ratio m. The distinctive features of the decay rates of the amplitudes of the KdV, mKdV, and Gardner solitons with a small effect of Landau damping are also studied in different parameter regimes. It is found that the Gardner soliton points to lower wave amplitudes than the KdV and mKdV solitons. The results may be useful for understanding the localization of solitary pulses and associated wave damping (collisionless) in laboratory and space plasmas (e.g., the F-ring of Saturn), in which the number density of free electrons is much smaller than that of ions and the heavy, micron seized dust grains are highly charged.

  18. Some approaches to substructure coupling with damping

    NASA Technical Reports Server (NTRS)

    Craig, R. R., Jr.; Bachmeyer, R. C.; Howsman, T. G.

    1986-01-01

    Time-domain and frequency-domain methods for coupling substructures with general linear damping are discussed. A time-domain method is presented which employs a state variable representation of each substructure. Also presented is a method which employs frequency-domain coupling together with DFT and FFT transformations to obtain transient response solutions.

  19. Chiral damping of magnetic domain walls.

    PubMed

    Jué, Emilie; Safeer, C K; Drouard, Marc; Lopez, Alexandre; Balint, Paul; Buda-Prejbeanu, Liliana; Boulle, Olivier; Auffret, Stephane; Schuhl, Alain; Manchon, Aurelien; Miron, Ioan Mihai; Gaudin, Gilles

    2016-03-01

    Structural symmetry breaking in magnetic materials is responsible for the existence of multiferroics, current-induced spin-orbit torques and some topological magnetic structures. In this Letter we report that the structural inversion asymmetry (SIA) gives rise to a chiral damping mechanism, which is evidenced by measuring the field-driven domain-wall (DW) motion in perpendicularly magnetized asymmetric Pt/Co/Pt trilayers. The DW dynamics associated with the chiral damping and those with Dzyaloshinskii-Moriya interaction (DMI) exhibit identical spatial symmetry. However, both scenarios are differentiated by their time reversal properties: whereas DMI is a conservative effect that can be modelled by an effective field, the chiral damping is purely dissipative and has no influence on the equilibrium magnetic texture. When the DW motion is modulated by an in-plane magnetic field, it reveals the structure of the internal fields experienced by the DWs, allowing one to distinguish the physical mechanism. The chiral damping enriches the spectrum of physical phenomena engendered by the SIA, and is essential for conceiving DW and skyrmion devices owing to its coexistence with DMI (ref. ). PMID:26689141

  20. DAMP signaling in fungal infections and diseases

    PubMed Central

    Cunha, Cristina; Carvalho, Agostinho; Esposito, Antonella; Bistoni, Francesco; Romani, Luigina

    2012-01-01

    Fungal infections and diseases predominantly affect patients with deregulated immunity. Compelling experimental and clinical evidence indicate that severe fungal diseases belong to the spectrum of fungus-related inflammatory diseases. Some degree of inflammation is required for protection during the transitional response occurring temporally between the rapid innate and slower adaptive response. However, progressive inflammation worsens disease and ultimately prevents pathogen eradication. The challenge now is to elucidate cellular and molecular pathways distinguishing protective vs. pathogenic inflammation to fungi. In addition to fungal ligands of pattern recognition receptors (pathogen-associated molecular patterns, PAMPs), several host-encoded proteins, the damage-associated molecular patterns (DAMPs), are released during tissue injury and activate innate recognition receptors. DAMPs have been shown to regulate inflammation in fungal diseases. The DAMP/receptor for advanced glycation end-products axis integrated with the PAMP/Toll-like receptors axis in the generation of the inflammatory response in experimental and clinical fungal pneumonia. These emerging themes better accommodate fungal pathogenesis in the face of high-level inflammation seen in several clinical settings and point to DAMP targeting as a novel immunomodulatory strategy in fungal diseases. PMID:22973279