Science.gov

Sample records for automated production line

  1. Automated solar panel assembly line

    NASA Technical Reports Server (NTRS)

    Somberg, H.

    1981-01-01

    The initial stage of the automated solar panel assembly line program was devoted to concept development and proof of approach through simple experimental verification. In this phase, laboratory bench models were built to demonstrate and verify concepts. Following this phase was machine design and integration of the various machine elements. The third phase was machine assembly and debugging. In this phase, the various elements were operated as a unit and modifications were made as required. The final stage of development was the demonstration of the equipment in a pilot production operation.

  2. Automated applications of the infrared imagers in the automotive assembly lines: products and process control

    NASA Astrophysics Data System (ADS)

    Omar, M. A.; Zhou, Y.; Liu, J.

    2007-04-01

    This work discusses the automated application of the micro-bolometric and the photonic cooled infrared arrays, in the context of the automotive assembly operations. Such usages comprise: static thermographic applications as in evaluating the protective coating coverage over steel-fuel tanks. Furthermore, this discussion includes a variety of processing algorithms dedicated for handling the acquired infrared sequences or scans; addressing challenges in emissivity variations, sensor saturation, and surrounding contribution, while introducing possible solution schemes. The physics and detection methodology are presented through modeling and simulation work.

  3. El Paso automates main line compressor stations

    SciTech Connect

    Kind, R.H. )

    1989-12-01

    This paper reports how an El Paso natural gas company has automated 27 compressor stations on its main line gas-transmission system, ahead of its 5-year schedule begun in 1984. The project involved the total automation (unmanned operation) of one reciprocating engine-driven compressor station and 21 turbine-driven compressor facilities; the semi-automation (computer-assisted operation) of six reciprocating engine-driven compressor stations; and the addition of a central control facility located in El Paso.

  4. Development of Automated Production Line Processes for Solar Brightfield Modules: Annual Technical Progress Report, 1 January 2003 -- 30 June 2004

    SciTech Connect

    Nowlan, M. J.; Murach, J. M.; Sutherland, S. F.; Miller, D. C.; Moore, S. B.; Hogan, S. J.

    2005-06-01

    This report describes how Spire Corporation is addressing the PV Manufacturing R&D project goals of improving photovoltaic (PV) manufacturing processes and products while reducing costs and providing a technology foundation that supports significant manufacturing scale-up. To accomplish this, we are focusing our efforts on the design of a large-area utility-scale module and the development of the necessary manufacturing techniques and equipment to manufacture such a module in a high-volume production environment. A three-phase program is under way for developing and demonstrating new automated systems for fabricating very large PV modules ideal for use in multi-megawatt grid-connected applications. We designed a large-area 800 W module and we are developing associated module production equipment that will minimize the total installed system cost for utility-scale PV arrays. Unique features of the module design include a cantilevered glass superstrate to reduce the glass thickness a nd internally laminated bypass diodes that simplify internal busing and junction-box designs. Other program activities include the development of automation for solar cell string inspections, string busing, materials lay-up, and lamination; enhancements to the lamination process; and performance testing of large-area modules.

  5. SAF line pellet gaging. [Secure Automated Fabrication

    SciTech Connect

    Jedlovec, D.R.; Bowen W.W.; Brown, R.L.

    1983-10-01

    Automated and remotely controlled pellet inspection operations will be utilized in the Secure Automated Fabrication (SAF) line. A prototypic pellet gage was designed and tested to verify conformance to the functions and requirements for measurement of diameter, surface flaws and weight-per-unit length.

  6. ALFA: Automated Line Fitting Algorithm

    NASA Astrophysics Data System (ADS)

    Wesson, R.

    2015-12-01

    ALFA fits emission line spectra of arbitrary wavelength coverage and resolution, fully automatically. It uses a catalog of lines which may be present to construct synthetic spectra, the parameters of which are then optimized by means of a genetic algorithm. Uncertainties are estimated using the noise structure of the residuals. An emission line spectrum containing several hundred lines can be fitted in a few seconds using a single processor of a typical contemporary desktop or laptop PC. Data cubes in FITS format can be analysed using multiple processors, and an analysis of tens of thousands of deep spectra obtained with instruments such as MUSE will take a few hours.

  7. Office Automation Boosts University's Productivity.

    ERIC Educational Resources Information Center

    School Business Affairs, 1986

    1986-01-01

    The University of Pittsburgh has a 2-year agreement designating the Xerox Corporation as the primary supplier of word processing and related office automation equipment in order to increase productivity and more efficient use of campus resources. (MLF)

  8. Start up testing for the secure automated fabrication line

    SciTech Connect

    Gerber, E.W.; Benson, E.M.; Dahl, R.E.

    1986-10-21

    The Secure Automated Fabrication (SAF) Line has been designed and built by Westinghouse Hanford Company for the Department of Energy at the Hanford Site near Richland, Washington. The SAF Line will provide the capability for remote manufacture of fuel for Liquid Metal Reactors, and will supply fuel for the Fast Flux Test Facility (FFTF). The SAF process is highly automated and represents a major advancement in nuclear fuel manufacturing, offering significant improvements in product quality, productivity, safety, and accountability of Special Nuclear Materials. The construction phase of the project is complete, and testing has been initiated to accomplish start up of the plant for manufacture of FFTF fuel. This paper describes the test methodology used for SAF Line start up.

  9. ALFA: an automated line fitting algorithm

    NASA Astrophysics Data System (ADS)

    Wesson, R.

    2016-03-01

    I present the automated line fitting algorithm, ALFA, a new code which can fit emission line spectra of arbitrary wavelength coverage and resolution, fully automatically. In contrast to traditional emission line fitting methods which require the identification of spectral features suspected to be emission lines, ALFA instead uses a list of lines which are expected to be present to construct a synthetic spectrum. The parameters used to construct the synthetic spectrum are optimized by means of a genetic algorithm. Uncertainties are estimated using the noise structure of the residuals. An emission line spectrum containing several hundred lines can be fitted in a few seconds using a single processor of a typical contemporary desktop or laptop PC. I show that the results are in excellent agreement with those measured manually for a number of spectra. Where discrepancies exist, the manually measured fluxes are found to be less accurate than those returned by ALFA. Together with the code NEAT, ALFA provides a powerful way to rapidly extract physical information from observations, an increasingly vital function in the era of highly multiplexed spectroscopy. The two codes can deliver a reliable and comprehensive analysis of very large data sets in a few hours with little or no user interaction.

  10. Productivity goals drive office automation

    NASA Technical Reports Server (NTRS)

    Bradley, A. P.; Kurzhals, P. R.

    1983-01-01

    Office automation (OA) steps being taken by NASA to improve efficiency in communications between centers and personnel are outlined. NASA centers are currently linked by satellite for electronic mail and scheduling through dumb and intelligent terminals. The implementation of teleconferencing with interactive graphics transmitted between dial-up terminals is being examined in a pilot program, and interactive data bases are already in operation, with an on-line summary data base being planned for NASA headquarters. The NASA Recon on-line service is operating with citations of over 2,200,000 aeronautics and astronautics research documents and 300,000 scientific books accessed by over 250 terminals around the U.S. The emphasis for all the OA systems is on user-friendly design and minimizing the required input for entry and access.

  11. Nuclear Energy Research Initiative Annual Report-Innovative Approaches to Automating QA/QC of Fuel Particle Production Using On-Line Nondestructive Methods for Higher Reliability.

    SciTech Connect

    Hockey, Ronald L.; Bond, Leonard J.; Ahmed, Salahuddin; Sandness, Gerald A.; Gray, Joseph N.; Batishko, Charles R.; Flake, Matthew; Panetta, Paul D.; Saurwein, John J.; Lowden, Richard A.; Good, Morris S.

    2004-04-20

    This document summarizes the activities performed and progress made in FY-03. Various approaches for automating the particle fuel production QC process using on-line nondestructive methods for higher reliability were evaluated. In this first-year of a three-year project, surrogate fuel particles made available for testing included leftovers from initial coater development runs. These particles had a high defect fraction and the particle properties spanned a wide range, providing the opportunity to examine worst-case conditions before refining the inspection methods to detect more subtle coating features. Particles specifically designed to evaluate the NDE methods being investigated under this project will be specified and fabricated at ORNL early next reporting period. The literature was reviewed for existing inspection technology and to identify many of the fuel particle conditions thought to degrade its performance. A modeling study, including the electromagnetic and techniques, showed that the in-line electromagnetic methods should provide measurable responses to missing layers, kernel diameter, and changes in coating layer thickness, with reasonable assumptions made for material conductivities. The modeling study for the ultrasonic methods provided the resonant frequencies that should be measured using the resonant ultrasound technique, and the results from these calculations were published in the proceedings for two conferences. The notion of a particle quality index to relate coating properties to fabrication process parameters was explored. Progress was made in understanding the fabrication process. GA identified key literature in this area and Saurwein (2003a) provided a literature review/summary. This literature has been reviewed. An approach previously applied to flexible manufacturing was adopted and the modification and development of the concepts to meet TRISO particle fuel manufacturing and QA/QC needs initiated. This approach establishes

  12. Development of Automated Production Line Processes for Solar Brightfield Modules: Final Annual Technical Progress Report, 1 July 2004 -- 15 October 2005

    SciTech Connect

    Nowlan, M. J.; Murach, J. M.; Sutherland, S. F.; Miller, D. C.; Moore S. B.; Hogan, S. J.

    2006-08-01

    Spire Corporation is addressing the Photovoltaic Manufacturing R&D project goals of improving photovoltaic (PV) manufacturing processes and products while reducing costs and providing a technology foundation that supports significant manufacturing scale-up. To accomplish this, we are focusing our efforts on the design of a large-area utility-scale module and the development of the necessary manufacturing techniques and equipment to manufacture such a module in a high-volume production environment. A three-phase program is under way for developing and demonstrating new automated systems for fabricating very large PV modules ideal for use in multi-megawatt grid-connected applications. We designed a large-area (1.57 m x 3.68 m) 800-W module, and we are developing associated module production equipment that will minimize the total installed system cost for utility-scale PV arrays. Activities in Phase 2 focused on the development of automation for module materials lay-up, cell string busing, and module lamination; enhancements to the cell stringing and lamination processes; and performance testing of large-area modules.

  13. Nuclear Energy Research Initiative Project No. 02 103 Innovative Low Cost Approaches to Automating QA/QC of Fuel Particle Production Using On Line Nondestructive Methods for Higher Reliability Final Project Report

    SciTech Connect

    Ahmed, Salahuddin; Batishko, Charles R.; Flake, Matthew; Good, Morris S.; Mathews, Royce; Morra, Marino; Panetta, Paul D.; Pardini, Allan F.; Sandness, Gerald A.; Tucker, Brian J.; Weier, Dennis R.; Hockey, Ronald L.; Gray, Joseph N.; Saurwein, John J.; Bond, Leonard J.; Lowden, Richard A.; Miller, James H.

    2006-02-28

    This Nuclear Energy Research Initiative (NERI) project was tasked with exploring, adapting, developing and demonstrating innovative nondestructive test methods to automate nuclear coated particle fuel inspection so as to provide the United States (US) with necessary improved and economical Quality Assurance and Control (QA/QC) that is needed for the fuels for several reactor concepts being proposed for both near term deployment [DOE NE & NERAC, 2001] and Generation IV nuclear systems. Replacing present day QA/QC methods, done manually and in many cases destructively, with higher speed automated nondestructive methods will make fuel production for advanced reactors economically feasible. For successful deployment of next generation reactors that employ particle fuels, or fuels in the form of pebbles based on particles, extremely large numbers of fuel particles will require inspection at throughput rates that do not significantly impact the proposed manufacturing processes. The focus of the project is nondestructive examination (NDE) technologies that can be automated for production speeds and make either: (I) On Process Measurements or (II) In Line Measurements. The inspection technologies selected will enable particle “quality” qualification as a particle or group of particles passes a sensor. A multiple attribute dependent signature will be measured and used for qualification or process control decisions. A primary task for achieving this objective is to establish standard signatures for both good/acceptable particles and the most problematic types of defects using several nondestructive methods.

  14. Multiresidue trace analysis of pharmaceuticals, their human metabolites and transformation products by fully automated on-line solid-phase extraction-liquid chromatography-tandem mass spectrometry.

    PubMed

    García-Galán, María Jesús; Petrovic, Mira; Rodríguez-Mozaz, Sara; Barceló, Damià

    2016-09-01

    A novel, fully automated analytical methodology based on dual column liquid chromatography coupled to tandem mass spectrometry (LC-LC-MS(2)) has been developed and validated for the analysis of 12 pharmaceuticals and 20 metabolites and transformation products in different types of water (influent and effluent wastewaters and surface water). Two LC columns were used - one for pre-concentration of the sample and the second for separation and analysis - so that water samples were injected directly in the chromatographic system. Besides the many advantages of the methodology, such as minimization of the sample volume required and its manipulation, both compounds ionized in positive and negative mode could be analyzed simultaneously without compromising the sensitivity. A comparative study of different mobile phases, gradients and LC pre-concentration columns was carried out to obtain the best analytical performance. Limits of detection (MLODs) achieved were in the low ngL(-1) range for all the compounds. The method was successfully applied to study the presence of the target analytes in different wastewater and surface water samples collected near the city of Girona (Catalonia, Spain). Data on the environmental presence and fate of pharmaceutical metabolites and TPs is still scarce, highlighting the relevance of the developed methodology. PMID:27343613

  15. Towards an automated intelligence product generation capability

    NASA Astrophysics Data System (ADS)

    Smith, Alison M.; Hawes, Timothy W.; Nolan, James J.

    2015-05-01

    Creating intelligence information products is a time consuming and difficult process for analysts faced with identifying key pieces of information relevant to a complex set of information requirements. Complicating matters, these key pieces of information exist in multiple modalities scattered across data stores, buried in huge volumes of data. This results in the current predicament analysts find themselves; information retrieval and management consumes huge amounts of time that could be better spent performing analysis. The persistent growth in data accumulation rates will only increase the amount of time spent on these tasks without a significant advance in automated solutions for information product generation. We present a product generation tool, Automated PrOduct Generation and Enrichment (APOGEE), which aims to automate the information product creation process in order to shift the bulk of the analysts' effort from data discovery and management to analysis. APOGEE discovers relevant text, imagery, video, and audio for inclusion in information products using semantic and statistical models of unstructured content. APOGEEs mixed-initiative interface, supported by highly responsive backend mechanisms, allows analysts to dynamically control the product generation process ensuring a maximally relevant result. The combination of these capabilities results in significant reductions in the time it takes analysts to produce information products while helping to increase the overall coverage. Through evaluation with a domain expert, APOGEE has been shown the potential to cut down the time for product generation by 20x. The result is a flexible end-to-end system that can be rapidly deployed in new operational settings.

  16. An automated Genomes-to-Natural Products platform (GNP) for the discovery of modular natural products

    PubMed Central

    Johnston, Chad W.; Skinnider, Michael A.; Wyatt, Morgan A.; Li, Xiang; Ranieri, Michael R. M.; Yang, Lian; Zechel, David L.; Ma, Bin; Magarvey, Nathan A.

    2015-01-01

    Bacterial natural products are a diverse and valuable group of small molecules, and genome sequencing indicates that the vast majority remain undiscovered. The prediction of natural product structures from biosynthetic assembly lines can facilitate their discovery, but highly automated, accurate, and integrated systems are required to mine the broad spectrum of sequenced bacterial genomes. Here we present a genome-guided natural products discovery tool to automatically predict, combinatorialize and identify polyketides and nonribosomal peptides from biosynthetic assembly lines using LC–MS/MS data of crude extracts in a high-throughput manner. We detail the directed identification and isolation of six genetically predicted polyketides and nonribosomal peptides using our Genome-to-Natural Products platform. This highly automated, user-friendly programme provides a means of realizing the potential of genetically encoded natural products. PMID:26412281

  17. Process development for automated solar cell and module production. Task 4: Automated array assembly

    NASA Technical Reports Server (NTRS)

    1980-01-01

    A process sequence which can be used in conjunction with automated equipment for the mass production of solar cell modules for terrestrial use was developed. The process sequence was then critically analyzed from a technical and economic standpoint to determine the technological readiness of certain process steps for implementation. The steps receiving analysis were: back contact metallization, automated cell array layup/interconnect, and module edge sealing. For automated layup/interconnect, both hard automation and programmable automation (using an industrial robot) were studied. The programmable automation system was then selected for actual hardware development.

  18. Product lines in hospitals.

    PubMed

    Weber, J R

    1986-01-01

    The findings presented in the case study demonstrate that financial analysis is only as valid as the assumptions it is based upon. The single most important task is the definition of the clinical product. All other associated data is suspect and can lead to serious errors in business decisions if the product is poorly defined. A comprehensive case mix system, integrated with other financial systems with flexible reporting capabilities is required. The hospital and physicians must be willing to invest the time and expense necessary to ensure that the data is reliable. The hospital must be prudent in determining what cost finding approach they will elect to use. Although the RCC method has some fundamental problems, care must be taken not to develop a cost system that will require a significant amount of effort to maintain. PMID:10311387

  19. Off-Line Catalog Production.

    ERIC Educational Resources Information Center

    OCLC Online Computer Library Center, Inc., Dublin, OH.

    The Ohio College Library Center's off-line catalog system is a limited technique for production of card catalogs. Unlike the on-line system, it cannotmake the resources of a region available to users in an individual institution, and it does not have the potential for significantly reducing rate of rise of library per-unit costs. In short, it is…

  20. Automation and control of off-planet oxygen production processes

    NASA Technical Reports Server (NTRS)

    Marner, W. J.; Suitor, J. W.; Schooley, L. S.; Cellier, F. E.

    1990-01-01

    This paper addresses several aspects of the automation and control of off-planet production processes. First, a general approach to process automation and control is discussed from the viewpoint of translating human process control procedures into automated procedures. Second, the control issues for the automation and control of off-planet oxygen processes are discussed. Sensors, instruments, and components are defined and discussed in the context of off-planet applications, and the need for 'smart' components is clearly established.

  1. Weld line detection and process control for welding automation

    NASA Astrophysics Data System (ADS)

    Yang, Sang-Min; Cho, Man-Ho; Lee, Ho-Young; Cho, Taik-Dong

    2007-03-01

    Welding has been widely used as a process to join metallic parts. But because of hazardous working conditions, workers tend to avoid this task. Techniques to achieve the automation are the recognition of joint line and process control. A CCD (charge coupled device) camera with a laser stripe was applied to enhance the automatic weld seam tracking in GMAW (gas metal arc welding). The adaptive Hough transformation having an on-line processing ability was used to extract laser stripes and to obtain specific weld points. The three-dimensional information obtained from the vision system made it possible to generate the weld torch path and to obtain information such as the width and depth of the weld line. In this study, a neural network based on the generalized delta rule algorithm was adapted to control the process of GMAW, such as welding speed, arc voltage and wire feeding speed. The width and depth of the weld joint have been selected as neurons in the input layer of the neural-network algorithm. The input variables, the width and depth of the weld joint, are determined by image information. The voltage, weld speed and wire feed rate are represented as the neurons in the output layer. The results of the neural-network learning applied to the welding are as follows: learning ratio 0.5, momentum ratio 0.7, the number of hidden layers 2 and the number of hidden units 8. They have significant influence on the weld quality.

  2. Process development for automated solar cell and module production. Task 4: automated array assembly

    SciTech Connect

    Hagerty, J.J.

    1980-06-30

    The scope of work under this contract involves specifying a process sequence which can be used in conjunction with automated equipment for the mass production of solar cell modules for terrestrial use. This process sequence is then critically analyzed from a technical and economic standpoint to determine the technological readiness of each process step for implementation. The process steps are ranked according to the degree of development effort required and according to their significance to the overall process. Under this contract the steps receiving analysis were: back contact metallization, automated cell array layup/interconnect, and module edge sealing. For automated layup/interconnect both hard automation and programmable automation (using an industrial robot) were studied. The programmable automation system was then selected for actual hardware development. Economic analysis using the SAMICS system has been performed during these studies to assure that development efforts have been directed towards the ultimate goal of price reduction. Details are given. (WHK)

  3. Automated yield forecasting in a high product mix ASIC facility

    NASA Astrophysics Data System (ADS)

    Barber, Duane; Giewont, Mark; Hanson, Jeff; Shen, Jun

    2005-05-01

    Yield forecasting is a key component in running a successful semiconductor fab. It is also a significant challenge for facilities such as ASIC houses, which fabricate a wide range of devices using multiple technologies. Yield forecasting takes on increased significance in these environments, with new products introduced frequently and many products running only in small numbers. An accurate yield prediction system can greatly accelerate the process of identifying design bugs, test program issues and process integration problems. To this end, we have constructed a forecasting model geared for our ASIC manufacturing line. The model will accommodate an arbitrary number of design and/or process elements, each with an associated defectivity term. In addition, we have automated the generation of the yield forecast through passively linking to the already existing EDA design tools and scripts used by LSI Logic. Once the model is constructed, an automated query engine can extract the design and process parameters for any requested device, insert the data into the forecasting model, and deliver the resulting yield prediction. The actual yield for any lot or group of lots may thus be compared to the forecast, greatly assisting yield enhancement activities. This is especially useful for prototype lots and low-volume devices, for which it eliminates a great deal of manual computation and searching of design files. Using the model in conjunction with the query engine, any deviations from expected yield performance are generated automatically, quickly and efficiently highlighting opportunities for improvement.

  4. On-line Automated Performance Diagnosis on Thousands of Processes

    SciTech Connect

    Roth, Philip C

    2006-01-01

    Performance analysis tools are critical for the effective use of large parallel computing resources, but existing tools have failed to address three problems that limit their scalability: (1) management and processing of the volume of performance data generated when monitoring a large number of application processes, (2) communication between a large number of tool components, and (3) presentation of performance data and analysis results for applications with a large number of processes. In this paper, we present a novel approach for finding performance problems in applications with a large number of processes that leverages our multicast and data aggregation infrastructure to address these three performance tool scalability barriers.First, we show how to design a scalable, distributed performance diagnosis facility. We demonstrate this design with an on-line, automated strategy for finding performance bottlenecks. Our strategy uses distributed, independent bottleneck search agents located in the tool agent processes that monitor running application processes. Second, we present a technique for constructing compact displays of the results of our bottleneck detection strategy. This technique, called the Sub-Graph Folding Algorithm, presents bottleneck search results using dynamic graphs that record the refinement of a bottleneck search. The complexity of the results graph is controlled by combining sub-graphs showing similar local application behavior into a composite sub-graph.Using an approach that combines these two synergistic parts, we performed bottleneck searches on programs with up to 1024 processes with no sign of tool resource saturation. With 1024 application processes, our visualization technique reduced a search results graph containing over 30,000 nodes to a single composite 44-node graph sub-graph showing the same qualitative performance information as the original graph.

  5. Automation Framework for Flight Dynamics Products Generation

    NASA Technical Reports Server (NTRS)

    Wiegand, Robert E.; Esposito, Timothy C.; Watson, John S.; Jun, Linda; Shoan, Wendy; Matusow, Carla

    2010-01-01

    XFDS provides an easily adaptable automation platform. To date it has been used to support flight dynamics operations. It coordinates the execution of other applications such as Satellite TookKit, FreeFlyer, MATLAB, and Perl code. It provides a mechanism for passing messages among a collection of XFDS processes, and allows sending and receiving of GMSEC messages. A unified and consistent graphical user interface (GUI) is used for the various tools. Its automation configuration is stored in text files, and can be edited either directly or using the GUI.

  6. Process development for automated solar cell and module production. Task 4: Automated array assembly

    NASA Technical Reports Server (NTRS)

    Hagerty, J. J.

    1981-01-01

    Progress in the development of automated solar cell and module production is reported. The unimate robot is programmed for the final 35 cell pattern to be used in the fabrication of the deliverable modules. The mechanical construction of the automated lamination station and final assembly station phases are completed and the first operational testing is underway. The final controlling program is written and optimized. The glass reinforced concrete (GRC) panels to be used for testing and deliverables are in production. Test routines are grouped together and defined to produce the final control program.

  7. PHOENIX: the production line for science data products at ESO

    NASA Astrophysics Data System (ADS)

    Hanuschik, Reinhard

    2015-12-01

    In the past two years ESO has installed a production line for level 2 science data products. Focussing on spectroscopic observations, these in-house generated data products are complementary to the externally provided data products from the imaging surveys. The production line combines mass production (more than one million spectra have been generated so far), previews, and quality control.

  8. Can machines see fecal contaminants? - Automated on-line poultry safety inspection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Automated food processing improves productivity with high quality control and benefits both producers and consumers. The poultry industry is one of the food industries that require automated processing for high throughput considering both safety and quality. Current poultry safety inspection is be...

  9. Automating the Production of Interactive Technical Documentation and EPSS.

    ERIC Educational Resources Information Center

    Emmott, Lyle C.

    1998-01-01

    Discusses how the production of IETM (interactive electronic technical manuals) and EPSS (electronic performance support systems) has been automated to give subject-matter experts the ability to mass-produce electronic documents, training, and training-support materials. Describes four projects involving object-relational database architecture.…

  10. Automated Analysis of Child Phonetic Production Using Naturalistic Recordings

    ERIC Educational Resources Information Center

    Xu, Dongxin; Richards, Jeffrey A.; Gilkerson, Jill

    2014-01-01

    Purpose: Conventional resource-intensive methods for child phonetic development studies are often impractical for sampling and analyzing child vocalizations in sufficient quantity. The purpose of this study was to provide new information on early language development by an automated analysis of child phonetic production using naturalistic…

  11. Aspect-Oriented Model-Driven Software Product Line Engineering

    NASA Astrophysics Data System (ADS)

    Groher, Iris; Voelter, Markus

    Software product line engineering aims to reduce development time, effort, cost, and complexity by taking advantage of the commonality within a portfolio of similar products. The effectiveness of a software product line approach directly depends on how well feature variability within the portfolio is implemented and managed throughout the development lifecycle, from early analysis through maintenance and evolution. This article presents an approach that facilitates variability implementation, management, and tracing by integrating model-driven and aspect-oriented software development. Features are separated in models and composed of aspect-oriented composition techniques on model level. Model transformations support the transition from problem to solution space models. Aspect-oriented techniques enable the explicit expression and modularization of variability on model, template, and code level. The presented concepts are illustrated with a case study of a home automation system.

  12. Unique automation system monitors south Florida production operations

    SciTech Connect

    Weaver, E.G.; Hildebrand, S.M.

    1982-06-01

    A stand-alone local automation system and company-wide design remote terminal units (RTU's) are combined into one system by employing a microprocessor-based control unit. The local system provides operational personnel with alarms, accumulators, analogs, and controls for daily real-time operations. These operational data points, through specialized software, are reformated and interfaced to pseudo-RTU's within the microprocessor memory. From the pseudo-RTU's, the information is transmitted to the company-wide computer production control (CPC) system. A description is given of this unique automation system, its equipment, functions, and operation. 2 refs.

  13. Automation or De-automation

    NASA Astrophysics Data System (ADS)

    Gorlach, Igor; Wessel, Oliver

    2008-09-01

    In the global automotive industry, for decades, vehicle manufacturers have continually increased the level of automation of production systems in order to be competitive. However, there is a new trend to decrease the level of automation, especially in final car assembly, for reasons of economy and flexibility. In this research, the final car assembly lines at three production sites of Volkswagen are analysed in order to determine the best level of automation for each, in terms of manufacturing costs, productivity, quality and flexibility. The case study is based on the methodology proposed by the Fraunhofer Institute. The results of the analysis indicate that fully automated assembly systems are not necessarily the best option in terms of cost, productivity and quality combined, which is attributed to high complexity of final car assembly systems; some de-automation is therefore recommended. On the other hand, the analysis shows that low automation can result in poor product quality due to reasons related to plant location, such as inadequate workers' skills, motivation, etc. Hence, the automation strategy should be formulated on the basis of analysis of all relevant aspects of the manufacturing process, such as costs, quality, productivity and flexibility in relation to the local context. A more balanced combination of automated and manual assembly operations provides better utilisation of equipment, reduces production costs and improves throughput.

  14. Application of distributive control in production automation

    SciTech Connect

    White, W.W.; Lockerd, C.R.

    1983-01-01

    When selecting an automatic well test system, Cities Service Co. realized it must meet the requirements defined by engineers and production personnel to document their present requirements and needs for future expansion. The concept of the system should be flexible enough to allow its implementation at any Cities Service production facility. The primary function of the Cities Service South Welch system was for automatic well test and CO/sub 2/ injection plant monitoring. The ability to implement features such as pump-off control, dynamometer cards, submersible pump control, waterflood plant control, and transmission of data to head office must be inherent in the system.

  15. An introduction to product line management.

    PubMed

    1991-01-01

    Management by product line has been used successfully in the manufacturing industry for decades. As the healthcare industry evolves, implementation of Product Line Management is emerging as one strategy to increase efficiency, effectiveness, and profitability. Product Line Management is an organizational scheme and alternative management system for focusing on the programmatic scope of operations in order to achieve particular objectives. Product Line Management in a hospital setting requires deviation from linear functional management paradigms to a reorganization in thinking that focuses on end-result objectives. This management structure recognizes the need to integrate operational and clinical management in response to market-driven forces from both the standpoint of packaging products for the marketplace and appropriate management of those products to improve organizational effectiveness and profitability. However, a "test of time" is necessary to evaluate whether Product Line Management has a positive impact on clinical intervention and outcome. Although Product Line Management seems to be a method to increase profitability, its effect on the quality of client care remains unclear. Speech-language pathologists and audiologists need to be well informed about Product Line Management in the healthcare industry, and should prepare for the possibility of participating within such a framework. Finally, speech-language pathologists and audiologists need to examine and consider their particular qualifications for assuming leadership within such a structure. PMID:2001266

  16. Automation in Animal Housing and Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Intensive, controlled environment animal production began modestly in the mid-20th century as poultry were brought indoors. While mankind had utilized structures to provide shelter for their animals for centuries, the availability of relatively inexpensive energy and the electrification of rural are...

  17. Performance evaluation of the croissant production line with reparable machines

    NASA Astrophysics Data System (ADS)

    Tsarouhas, Panagiotis H.

    2015-09-01

    In this study, the analytical probability models for an automated serial production system, bufferless that consists of n-machines in series with common transfer mechanism and control system was developed. Both time to failure and time to repair a failure are assumed to follow exponential distribution. Applying those models, the effect of system parameters on system performance in actual croissant production line was studied. The production line consists of six workstations with different numbers of reparable machines in series. Mathematical models of the croissant production line have been developed using Markov process. The strength of this study is in the classification of the whole system in states, representing failures of different machines. Failure and repair data from the actual production environment have been used to estimate reliability and maintainability for each machine, workstation, and the entire line is based on analytical models. The analysis provides a useful insight into the system's behaviour, helps to find design inherent faults and suggests optimal modifications to upgrade the system and improve its performance.

  18. Performance evaluation of the croissant production line with reparable machines

    NASA Astrophysics Data System (ADS)

    Tsarouhas, Panagiotis H.

    2014-09-01

    In this study, the analytical probability models for an automated serial production system, bufferless that consists of n-machines in series with common transfer mechanism and control system was developed. Both time to failure and time to repair a failure are assumed to follow exponential distribution. Applying those models, the effect of system parameters on system performance in actual croissant production line was studied. The production line consists of six workstations with different numbers of reparable machines in series. Mathematical models of the croissant production line have been developed using Markov process. The strength of this study is in the classification of the whole system in states, representing failures of different machines. Failure and repair data from the actual production environment have been used to estimate reliability and maintainability for each machine, workstation, and the entire line is based on analytical models. The analysis provides a useful insight into the system's behaviour, helps to find design inherent faults and suggests optimal modifications to upgrade the system and improve its performance.

  19. Development of a Production Ready Automated Wire Delivery System

    NASA Technical Reports Server (NTRS)

    1997-01-01

    The current development effort is a Phase 3 research study entitled "A Production Ready Automated Wire Delivery System", contract number NAS8-39933, awarded to Nichols Research Corporation (NRC). The goals of this research study were to production harden the existing Automated Wire Delivery (AWDS) motion and sensor hardware and test the modified AWDS in a range of welding applications. In addition, the prototype AWDS controller would be moved to the VME bus platform by designing, fabricating and testing a single board VME bus AWDS controller. This effort was to provide an AWDS that could transition from the laboratory environment to production operations. The project was performed in two development steps. Step 1 modified and tested an improved MWG. Step 2 developed and tested the AWDS single board VME bus controller. Step 3 installed the Wire Pilot in a Weld Controller with the imbedded VME bus controller.

  20. Automated Sample Exchange Robots for the Structural Biology Beam Lines at the Photon Factory

    SciTech Connect

    Hiraki, Masahiko; Watanabe, Shokei; Yamada, Yusuke; Matsugaki, Naohiro; Igarashi, Noriyuki; Gaponov, Yurii; Wakatsuki, Soichi

    2007-01-19

    We are now developing automated sample exchange robots for high-throughput protein crystallographic experiments for onsite use at synchrotron beam lines. It is part of the fully automated robotics systems being developed at the Photon Factory, for the purposes of protein crystallization, monitoring crystal growth, harvesting and freezing crystals, mounting the crystals inside a hutch and for data collection. We have already installed the sample exchange robots based on the SSRL automated mounting system at our insertion device beam lines BL-5A and AR-NW12A at the Photon Factory. In order to reduce the time required for sample exchange further, a prototype of a double-tonged system was developed. As a result of preliminary experiments with double-tonged robots, the sample exchange time was successfully reduced from 70 seconds to 10 seconds with the exception of the time required for pre-cooling and warming up the tongs.

  1. ON-LINE MEASUREMENT OF NITROUS OXIDE FROM COMBUSTION SOURCES BY AUTOMATED GAS CHROMATOGRAPHY

    EPA Science Inventory

    The paper discusses on-line measurement of nitrous oxide (N2O) from combustion sources by automated gas chromatography. ossil fuel combustion is suspected of contributing to measured increases in the ambient concentrations of N2O. haracterization of N2O emissions from fossil fuel...

  2. Automated Single-Kernel Sorting to Select for Quality Traits in Wheat Breeding Lines

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An automated single kernel near-infrared system was used to select kernels to enhance the end-use quality of hard red wheat breeder samples. Twenty breeding populations and advanced lines were sorted for hardness index, protein content, and kernel color. To determine if the phenotypic sorting was b...

  3. Human Communication Needs and Organizational Productivity: The Potential Impact of Office Automation.

    ERIC Educational Resources Information Center

    Culnan, Mary J.; Bair, James H.

    1983-01-01

    This survey of potential impacts of office automation on organizational communication and productivity covers the following--(1) the relationship between office automation and organizational communication; (2) communication variables relevant to office automation; (3) benefits and caveats related to implementation of office automation. Thirty-four…

  4. Automation of production of semiconductor power devices

    NASA Astrophysics Data System (ADS)

    Kyuts, R. V.; Toomsoo, G. K.

    1984-10-01

    An informational consultative automatic system for control of technological processes is being developed. Then automatic control systems for groups of technological processes are being developed, essentially with a computer base. Its accounting functions include tracking of parts, statistical analysis of product lots, inspection and correction for excessive reject rate. Its control functions include adaptive simulation of technological processes, with stabilization and correction, and predicting characteristics. The software consists of general and special programs. The hardware consists of modular computers with expanded memory on three magnetic disks and four magnetic tapes, workshop terminals with teletypes, and testers. The data processing part consists of minicomputer with peripheral equipment. The testing part includes means of transporting as well as heating and cooling the test samples, and inserting them into the test circuit adequate electrical contact.

  5. Automated ocean color product validation for the Southern California Bight

    NASA Astrophysics Data System (ADS)

    Davis, Curtiss O.; Tufillaro, Nicholas; Jones, Burt; Arnone, Robert

    2012-06-01

    Automated match ups allow us to maintain and improve the products of current satellite ocean color sensors (MODIS, MERIS), and new sensors (VIIRS). As part of the VIIRS mission preparation, we have created a web based automated match up tool that provides access to searchable fields for date, site, and products, and creates match-ups between satellite (MODIS, MERIS, VIIRS), and in-situ measurements (HyperPRO and SeaPRISM). The back end of the system is a 'mySQL' database, and the front end is a `php' web portal with pull down menus for searchable fields. Based on selections, graphics are generated showing match-ups and statistics, and ascii files are created for downloads for the matchup data. Examples are shown for matching the satellite data with the data from Platform Eureka SeaPRISM off L.A. Harbor in the Southern California Bight.

  6. Automated Production of Movies on a Cluster of Computers

    NASA Technical Reports Server (NTRS)

    Nail, Jasper; Le, Duong; Nail, William L.; Nail, William

    2008-01-01

    A method of accelerating and facilitating production of video and film motion-picture products, and software and generic designs of computer hardware to implement the method, are undergoing development. The method provides for automation of most of the tedious and repetitive tasks involved in editing and otherwise processing raw digitized imagery into final motion-picture products. The method was conceived to satisfy requirements, in industrial and scientific testing, for rapid processing of multiple streams of simultaneously captured raw video imagery into documentation in the form of edited video imagery and video derived data products for technical review and analysis. In the production of such video technical documentation, unlike in production of motion-picture products for entertainment, (1) it is often necessary to produce multiple video derived data products, (2) there are usually no second chances to repeat acquisition of raw imagery, (3) it is often desired to produce final products within minutes rather than hours, days, or months, and (4) consistency and quality, rather than aesthetics, are the primary criteria for judging the products. In the present method, the workflow has both serial and parallel aspects: processing can begin before all the raw imagery has been acquired, each video stream can be subjected to different stages of processing simultaneously on different computers that may be grouped into one or more cluster(s), and the final product may consist of multiple video streams. Results of processing on different computers are shared, so that workers can collaborate effectively.

  7. Soliton production with nonlinear homogeneous lines

    SciTech Connect

    Elizondo-Decanini, Juan M.; Coleman, Phillip D.; Moorman, Matthew W.; Petney, Sharon Joy Victor; Dudley, Evan C.; Youngman, Kevin; Penner, Tim Dwight; Fang, Lu; Myers, Katherine M.

    2015-11-24

    Low- and high-voltage Soliton waves were produced and used to demonstrate collision and compression using diode-based nonlinear transmission lines. Experiments demonstrate soliton addition and compression using homogeneous nonlinear lines. We built the nonlinear lines using commercially available diodes. These diodes are chosen after their capacitance versus voltage dependence is used in a model and the line design characteristics are calculated and simulated. Nonlinear ceramic capacitors are then used to demonstrate high-voltage pulse amplification and compression. The line is designed such that a simple capacitor discharge, input signal, develops soliton trains in as few as 12 stages. We also demonstrated output voltages in excess of 40 kV using Y5V-based commercial capacitors. The results show some key features that determine efficient production of trains of solitons in the kilovolt range.

  8. Soliton production with nonlinear homogeneous lines

    DOE PAGESBeta

    Elizondo-Decanini, Juan M.; Coleman, Phillip D.; Moorman, Matthew W.; Petney, Sharon Joy Victor; Dudley, Evan C.; Youngman, Kevin; Penner, Tim Dwight; Fang, Lu; Myers, Katherine M.

    2015-11-24

    Low- and high-voltage Soliton waves were produced and used to demonstrate collision and compression using diode-based nonlinear transmission lines. Experiments demonstrate soliton addition and compression using homogeneous nonlinear lines. We built the nonlinear lines using commercially available diodes. These diodes are chosen after their capacitance versus voltage dependence is used in a model and the line design characteristics are calculated and simulated. Nonlinear ceramic capacitors are then used to demonstrate high-voltage pulse amplification and compression. The line is designed such that a simple capacitor discharge, input signal, develops soliton trains in as few as 12 stages. We also demonstrated outputmore » voltages in excess of 40 kV using Y5V-based commercial capacitors. The results show some key features that determine efficient production of trains of solitons in the kilovolt range.« less

  9. Flight flutter testing technology at Grumman. [automated telemetry station for on line data reduction

    NASA Technical Reports Server (NTRS)

    Perangelo, H. J.; Milordi, F. W.

    1976-01-01

    Analysis techniques used in the automated telemetry station (ATS) for on line data reduction are encompassed in a broad range of software programs. Concepts that form the basis for the algorithms used are mathematically described. The control the user has in interfacing with various on line programs is discussed. The various programs are applied to an analysis of flight data which includes unimodal and bimodal response signals excited via a swept frequency shaker and/or random aerodynamic forces. A nonlinear response error modeling analysis approach is described. Preliminary results in the analysis of a hard spring nonlinear resonant system are also included.

  10. Automation and robotics - Key to productivity. [in industry and space

    NASA Technical Reports Server (NTRS)

    Cohen, A.

    1985-01-01

    The automated and robotic systems requirements of the NASA Space Station are prompted by maintenance, repair, servicing and assembly requirements. Trend analyses, fault diagnoses, and subsystem status assessments for the Station's electrical power, guidance, navigation, control, data management and environmental control subsystems will be undertaken by cybernetic expert systems; this will reduce or eliminate on-board or ground facility activities that would otherwise be essential, enhancing system productivity. Additional capabilities may also be obtained through the incorporation of even a limited amount of artificial intelligence in the controllers of the various Space Station systems.

  11. Reference line extraction for automated data-entry system using wavelet transform

    NASA Astrophysics Data System (ADS)

    Chitwong, Sakreya; Phonsri, Seksan; Thitimajshima, Punya

    1999-12-01

    It is common that most document forms opt for the use of straight line as a reference position for filled information. The automated data-entry systems of such documents require an ability to search these reference lines so that the location of information in the forms can be known. This paper proposes a wavelet-based algorithm for extracting these reference lines in business forms. Stationary wavelet transform is used to transform a gray-level document image into different frequency-band images. The horizontal detail subband is then selected and passed through a post-processing to produce a binary bitmap of reference lines. The experimental results on synthetic and real document images will be given to illustrate the usefulness of such an algorithm.

  12. The Future of Product Line Development at NASA

    NASA Technical Reports Server (NTRS)

    Lutz, Robyn R.

    2006-01-01

    This viewgraph presentation reviews NASA's software production in the light of a product line similar to a commercial product line. The authors propose to identify, investigate, evaluate and apply product-line engineering techniques to NASA's product lines in order to improve the timeliness, robustness and effectiveness of these future systems.

  13. On the Automated and Objective Detection of Emission Lines in Faint-Object Spectroscopy

    NASA Astrophysics Data System (ADS)

    Hong, Sungryong; Dey, Arjun; Prescott, Moire K. M.

    2014-11-01

    Modern spectroscopic surveys produce large spectroscopic databases, generally with sizes well beyond the scope of manual investigation. The need arises, therefore, for an automated line detection method with objective indicators for detection significance. In this paper, we present an automated and objective method for emission line detection in spectroscopic surveys and apply this technique to observed spectra from a Lyα emitter survey at z ~ 2.7, obtained with the Hectospec spectrograph on the MMT Observatory (MMTO). The basic idea is to generate on-source (signal plus noise) and off-source (noise only) mock observations using Monte Carlo simulations, and calculate completeness and reliability values, (C,R), for each simulated signal. By comparing the detections from real data with the Monte Carlo results, we assign the completeness and reliability values to each real detection. From 1574 spectra, we obtain 881 raw detections and, by removing low reliability detections, we finalize 652 detections from an automated pipeline. Most of high completeness and reliability detections, (C,R) ≈ (1.0,1.0), are robust detections when visually inspected; the low C and R detections are also marginal on visual inspection. This method of detecting faint sources is dependent on the accuracy of the sky subtraction.

  14. A power line data communication interface using spread spectrum technology in home automation

    SciTech Connect

    Shwehdi, M.H.; Khan, A.Z.

    1996-07-01

    Building automation technology is rapidly developing towards more reliable communication systems, devices that control electronic equipments. These equipment if controlled leads to efficient energy management, and savings on the monthly electricity bill. Power Line communication (PLC) has been one of the dreams of the electronics industry for decades, especially for building automation. It is the purpose of this paper to demonstrate communication methods among electronic control devices through an AC power line carrier within the buildings for more efficient energy control. The paper outlines methods of communication over a powerline, namely the X-10 and CE bus. It also introduces the spread spectrum technology as to increase speed to 100--150 times faster than the X-10 system. The powerline carrier has tremendous applications in the field of building automation. The paper presents an attempt to realize a smart house concept, so called, in which all home electronic devices from a coffee maker to a water heater microwave to chaos robots will be utilized by an intelligent network whenever one wishes to do so. The designed system may be applied very profitably to help in energy management for both customer and utility.

  15. Lutetium-177 DOTATATE Production with an Automated Radiopharmaceutical Synthesis System

    PubMed Central

    Aslani, Alireza; Snowdon, Graeme M; Bailey, Dale L; Schembri, Geoffrey P; Bailey, Elizabeth A; Pavlakis, Nick; Roach, Paul J

    2015-01-01

    Objective(s): Peptide Receptor Radionuclide Therapy (PRRT) with yttrium-90 (90Y) and lutetium-177 (177Lu)-labelled SST analogues are now therapy option for patients who have failed to respond to conventional medical therapy. In-house production with automated PRRT synthesis systems have clear advantages over manual methods resulting in increasing use in hospital-based radiopharmacies. We report on our one year experience with an automated radiopharmaceutical synthesis system. Methods: All syntheses were carried out using the Eckert & Ziegler Eurotope’s Modular-Lab Pharm Tracer® automated synthesis system. All materials and methods used were followed as instructed by the manufacturer of the system (Eckert & Ziegler Eurotope, Berlin, Germany). Sterile, GMP-certified, no-carrier added (NCA) 177Lu was used with GMP-certified peptide. An audit trail was also produced and saved by the system. The quality of the final product was assessed after each synthesis by ITLC-SG and HPLC methods. Results: A total of 17 [177Lu]-DOTATATE syntheses were performed between August 2013 and December 2014. The amount of radioactive [177Lu]-DOTATATE produced by each synthesis varied between 10-40 GBq and was dependant on the number of patients being treated on a given day. Thirteen individuals received a total of 37 individual treatment administrations in this period. There were no issues and failures with the system or the synthesis cassettes. The average radiochemical purity as determined by ITLC was above 99% (99.8 ± 0.05%) and the average radiochemical purity as determined by HPLC technique was above 97% (97.3 ± 1.5%) for this period. Conclusions: The automated synthesis of [177Lu]-DOTATATE using Eckert & Ziegler Eurotope’s Modular-Lab Pharm Tracer® system is a robust, convenient and high yield approach to the radiolabelling of DOTATATE peptide benefiting from the use of NCA 177Lu and almost negligible radiation exposure of the operators. PMID:27408890

  16. SCOUSE: Semi-automated multi-COmponent Universal Spectral-line fitting Engine

    NASA Astrophysics Data System (ADS)

    Henshaw, J. D.; Longmore, S. N.; Kruijssen, J. M. D.; Davies, B.; Bally, J.; Barnes, A.; Battersby, C.; Burton, M.; Cunningham, M. R.; Dale, J. E.; Ginsburg, A.; Immer, K.; Jones, P. A.; Kendrew, S.; Mills, E. A. C.; Molinari, S.; Moore, T. J. T.; Ott, J.; Pillai, T.; Rathborne, J.; Schilke, P.; Schmiedeke, A.; Testi, L.; Walker, D.; Walsh, A.; Zhang, Q.

    2016-01-01

    The Semi-automated multi-COmponent Universal Spectral-line fitting Engine (SCOUSE) is a spectral line fitting algorithm that fits Gaussian files to spectral line emission. It identifies the spatial area over which to fit the data and generates a grid of spectral averaging areas (SAAs). The spatially averaged spectra are fitted according to user-provided tolerance levels, and the best fit is selected using the Akaike Information Criterion, which weights the chisq of a best-fitting solution according to the number of free-parameters. A more detailed inspection of the spectra can be performed to improve the fit through an iterative process, after which SCOUSE integrates the new solutions into the solution file.

  17. Automated decomposition algorithm for Raman spectra based on a Voigt line profile model.

    PubMed

    Chen, Yunliang; Dai, Liankui

    2016-05-20

    Raman spectra measured by spectrometers usually suffer from band overlap and random noise. In this paper, an automated decomposition algorithm based on a Voigt line profile model for Raman spectra is proposed to solve this problem. To decompose a measured Raman spectrum, a Voigt line profile model is introduced to parameterize the measured spectrum, and a Gaussian function is used as the instrumental broadening function. Hence, the issue of spectral decomposition is transformed into a multiparameter optimization problem of the Voigt line profile model parameters. The algorithm can eliminate instrumental broadening, obtain a recovered Raman spectrum, resolve overlapping bands, and suppress random noise simultaneously. Moreover, the recovered spectrum can be decomposed to a group of Lorentzian functions. Experimental results on simulated Raman spectra show that the performance of this algorithm is much better than a commonly used blind deconvolution method. The algorithm has also been tested on the industrial Raman spectra of ortho-xylene and proved to be effective. PMID:27411136

  18. Design of Control System for Flexible Packaging Bags Palletizing Production Line Based on PLC

    NASA Astrophysics Data System (ADS)

    Zheng, Huiping; Chen, Lin; Zhao, Xiaoming; Liu, Zhanyang

    Flexible packaging bags palletizing production line is to put the bags in the required area according to particular order and size, in order to finish handling, storage, loading and unloading, transportation and other logistics work of goods. Flexible packaging bags palletizing line is composed of turning bags mechanism, shaping mechanism, indexing mechanism, marshalling mechanism, pushing bags mechanism, pressing bags mechanism, laminating mechanism, elevator, tray warehouse, tray conveyor and loaded tray conveyor. Whether the whole production line can smoothly run depends on each of the above equipment and precision control among them. In this paper the technological process and the control logic of flexible packaging bags palletizing production line is introduced. Palletizing process of the production line realized automation by means of a control system based on programmable logic controller (PLC). It has the advantages of simple structure, reliable and easy maintenance etc.

  19. An automated system for measuring the mass flowrate of powders in transport lines

    NASA Astrophysics Data System (ADS)

    Littman, Howard; Morgan, Morris B., III; Prapas, Demetrios K.; Rubel, Glen O.

    1990-08-01

    A new automated particle transport (APT) system has been developed for studying the dissemination of bulk powders into deagglomerated aerosols. It consists of a 1.12-inch ID transport line with a spout-fluidized bed feeder. The particles are transported from an aerated annulus into the transport line and collected in a closed can or bag filter. Two separate feed lines supply the air necessary to operate the transport line and aerate the particles in order that they flow smoothly into the transport line. An IBM PC AT computer clone equipped with a data translation DT 2806 multifunction input-output board and A to D and D to A modules (DTX 311 and 328) is used for both control and data acquisition. A fluid mechanical model of the flow has been developed and the APT system will be used to verify it. Experiments will be conducted to measure the choking velocity, drag coefficient, fluid and particle flowrates, and pressure distribution in the line.

  20. Towards more reliable automated multi-dose dispensing: retrospective follow-up study on medication dose errors and product defects.

    PubMed

    Palttala, Iida; Heinämäki, Jyrki; Honkanen, Outi; Suominen, Risto; Antikainen, Osmo; Hirvonen, Jouni; Yliruusi, Jouko

    2013-03-01

    To date, little is known on applicability of different types of pharmaceutical dosage forms in an automated high-speed multi-dose dispensing process. The purpose of the present study was to identify and further investigate various process-induced and/or product-related limitations associated with multi-dose dispensing process. The rates of product defects and dose dispensing errors in automated multi-dose dispensing were retrospectively investigated during a 6-months follow-up period. The study was based on the analysis of process data of totally nine automated high-speed multi-dose dispensing systems. Special attention was paid to the dependence of multi-dose dispensing errors/product defects and pharmaceutical tablet properties (such as shape, dimensions, weight, scored lines, coatings, etc.) to profile the most suitable forms of tablets for automated dose dispensing systems. The relationship between the risk of errors in dose dispensing and tablet characteristics were visualized by creating a principal component analysis (PCA) model for the outcome of dispensed tablets. The two most common process-induced failures identified in the multi-dose dispensing are predisposal of tablet defects and unexpected product transitions in the medication cassette (dose dispensing error). The tablet defects are product-dependent failures, while the tablet transitions are dependent on automated multi-dose dispensing systems used. The occurrence of tablet defects is approximately twice as common as tablet transitions. Optimal tablet preparation for the high-speed multi-dose dispensing would be a round-shaped, relatively small/middle-sized, film-coated tablet without any scored line. Commercial tablet products can be profiled and classified based on their suitability to a high-speed multi-dose dispensing process. PMID:22458299

  1. Automated genome mining of ribosomal peptide natural products

    SciTech Connect

    Mohimani, Hosein; Kersten, Roland; Liu, Wei; Wang, Mingxun; Purvine, Samuel O.; Wu, Si; Brewer, Heather M.; Pasa-Tolic, Ljiljana; Bandeira, Nuno; Moore, Bradley S.; Pevzner, Pavel A.; Dorrestein, Pieter C.

    2014-07-31

    Ribosomally synthesized and posttranslationally modified peptides (RiPPs), especially from microbial sources, are a large group of bioactive natural products that are a promising source of new (bio)chemistry and bioactivity (1). In light of exponentially increasing microbial genome databases and improved mass spectrometry (MS)-based metabolomic platforms, there is a need for computational tools that connect natural product genotypes predicted from microbial genome sequences with their corresponding chemotypes from metabolomic datasets. Here, we introduce RiPPquest, a tandem mass spectrometry database search tool for identification of microbial RiPPs and apply it for lanthipeptide discovery. RiPPquest uses genomics to limit search space to the vicinity of RiPP biosynthetic genes and proteomics to analyze extensive peptide modifications and compute p-values of peptide-spectrum matches (PSMs). We highlight RiPPquest by connection of multiple RiPPs from extracts of Streptomyces to their gene clusters and by the discovery of a new class III lanthipeptide, informatipeptin, from Streptomyces viridochromogenes DSM 40736 as the first natural product to be identified in an automated fashion by genome mining. The presented tool is available at cy-clo.ucsd.edu.

  2. Automation of beam based alignment for the PAL-XFEL undulator line

    NASA Astrophysics Data System (ADS)

    Parc, Yong Woon; Hwang, Ilmoon; Han, Jang-Hui; Ko, In Soo

    2015-05-01

    An automated method of quadrupole beam based alignment in an X-ray free electron laser (XFEL) undulator line is proposed. To realize the automatic beam based alignment (BBA) independently of operator's skill and efforts, the procedure is simplified. Simulation results on the BBA technique using two alignment stages for the Pohang Accelerator Laboratory XFEL's (PAL-XFEL) undulator line is carried out. After a standard mechanical alignment the quadrupoles are expected to be aligned to 100 μm rms from our previous experience. A rough alignment method in this study is able to align the quadrupoles to about 40 μm rms without any pre-handling of electron beam orbit or quadrupoles position. At the second stage, the singular value decomposition (SVD) method is applied to perform a fine alignment in which the trajectory of an electron beam is controlled within 5 μm rms to the ideal orbit. All procedures are programmable to perform the BBA automatically.

  3. WARP (workflow for automated and rapid production): a framework for end-to-end automated digital print workflows

    NASA Astrophysics Data System (ADS)

    Joshi, Parag

    2006-02-01

    Publishing industry is experiencing a major paradigm shift with the advent of digital publishing technologies. A large number of components in the publishing and print production workflow are transformed in this shift. However, the process as a whole requires a great deal of human intervention for decision making and for resolving exceptions during job execution. Furthermore, a majority of the best-of-breed applications for publishing and print production are intrinsically designed and developed to be driven by humans. Thus, the human-intensive nature of the current prepress process accounts for a very significant amount of the overhead costs in fulfillment of jobs on press. It is a challenge to automate the functionality of applications built with the model of human driven exectution. Another challenge is to orchestrate various components in the publishing and print production pipeline such that they work in a seamless manner to enable the system to perform automatic detection of potential failures and take corrective actions in a proactive manner. Thus, there is a great need for a coherent and unifying workflow architecture that streamlines the process and automates it as a whole in order to create an end-to-end digital automated print production workflow that does not involve any human intervention. This paper describes an architecture and building blocks that lay the foundation for a plurality of automated print production workflows.

  4. Process Development for Automated Solar Cell and Module Production. Task 4: Automated Array Assembly

    NASA Technical Reports Server (NTRS)

    Hagerty, J. J.

    1981-01-01

    The Automated Lamination Station is mechanically complete and is currently undergoing final wiring. The high current driver and isolator boards have been completed and installed, and the main interface board is under construction. The automated vacuum chamber has had a minor redesign to increase stiffness and improve the cover open/close mechanism. Design of the Final Assembly Station has been completed and construction is underway.

  5. Automating Mapping Production for the Enterprise: from Contract to Delivery

    NASA Astrophysics Data System (ADS)

    Uebbing, R.; Xie, C.; Beshah, B.; Welter, J.

    2012-07-01

    The ever increasing volume and quality of geospatial data has created new challenges for mapping companies. Due to increased image resolution, fusion of different data sources and more frequent data update requirements, mapping production is forced to streamline the work flow to meet client deadlines. But the data volume alone is not the only barrier for an efficient production work flow. Processing geospatial information traditionally uses domain and vendor specific applications that do not interface with each other, often leading to data duplication and therefore creating sources for error. Also, it creates isolation between different departments within a mapping company resulting in additional communication barriers. North West Geomatics has designed and implemented a data centric enterprise solution for the flight acquisition and production work flow to combat the above challenges. A central data repository containing not only geospatial data in the strictest sense such as images, vector layers and 3D point clouds, but also other information such as product specifications, client requirements, flight acquisition data, production resource usage and much more has been deployed at the company. As there is only one instance of the database shared throughout the whole organization it allows all employees, given they have been granted the appropriate permission, to view the current status of any project with a graphical and table based interface through its life cycle from sales, through flight acquisition, production and product delivery. Not only can users track progress and status of various work flow steps, but the system also allows users and applications to actively schedule or start specific production steps such as data ingestion and triangulation with many other steps (orthorectification, mosaicing, accounting, etc.) in the planning stages. While the complete system is exposed to the users through a web interface and therefore allowing outside customers to

  6. Environmental friendly automated line for recovering the cabinet of waste refrigerator.

    PubMed

    Ruan, Jujun; Xu, Zhenming

    2011-11-01

    A large amount of hazardous trichloromonofluoromethane (CFC-11) is contained in the polyurethane (PUR) foam of refrigerator cabinet. How to recover the cabinet of waste refrigerator with environmental awareness has been the pressing problem in the treatment of waste refrigerator. The published literature of waste refrigerator cabinets recovering mainly focuses on policy making and conceptual design. Little information relates to the production line of waste refrigerator cabinet recovering and its detailed operation, which is the urgent needed literature. In this paper, an environmental friendly production line for recovering the cabinet of waste refrigerators was reported for helping enterprise or government to construct line to recover the obsolete refrigerators. It included closed shearing process, activated carbon fiber absorption (ACFA) process, air current separation, and magnetic/eddy current separation. The optimized operating parameters of every process were obtained from the experiments. Then, experiment of recovering 50 waste refrigerator cabinets by the production line was performed in factory. Mass balances of the recovered materials were carried out. The result shown the recovery rate of waste refrigerator cabinet could reach 97.6%. Finally, comparison between the production line and other recovery technologies was made. The results indicated that the proposed line provided environment-friendly recovery of waste refrigerator cabinets while achieving low cost of production. PMID:21782408

  7. Special steel production on common carbon steel production line

    NASA Astrophysics Data System (ADS)

    Pi, Huachun; Han, Jingtao; Hu, Haiping; Bian, Ruisheng; Kang, Jianjun; Xu, Manlin

    2004-06-01

    The equipment and technology of small bar tandem rolling line of Shijiazhuang Iron & Steel Co. in China has reached the 90's international advanced level in the 20th century, but products on the line are mostly of common carbon steel. Currently there are few steel plants in China to produce 45 steel bars for cold drawing, which is a kind of shortage product. Development of 45 steel for cold drawing has a wide market outlook in China. In this paper, continuous cooling transformation (CCT) curve of 45 steel for cold drawing used for rolling was set out first. According to the CCT curve, we determined some key temperature points such as Ac3 temperature and Ac1 temperature during the cooling procedure and discussed the precipitation microstructure at different cooling rate. Then by studying thermal treatment process of 45 steel bars for cold drawing, the influence of cooling time on microstructure was analyzed and the optimum cooling speed has been found. All results concluded from the above studies are the basis of regulating controlled cooling process of 45 steel bars for cold drawing. Finally, the feasible production process of 45 steel bars for cold drawing on common carbon steel production line combined with the field condition was recommended.

  8. Automated flow for site definition and CD measurement with a SEM for use in mask production

    NASA Astrophysics Data System (ADS)

    Rotsch, Christian; Haffner, Henning; Ruebekohl, Christian; Buechner, Bettine

    2002-12-01

    The continuous tightening of CD and registration specifications demands most advanced metrology equipment and highly sophisticated logistics and measurement strategies. Not only the smallness of structures but also the increasing number of measurement sites is a challenge. Until recently, CD measurements in mask mass production were done at a handful of different positions using mainly optical microscopy. The measurement locations were usually picked randomly according to the visual image and some general rules that were agreed upon between lithographers and the mask shop. In this paper we describe a flow for SEM based CD measurement in automated production. A new type of instances is introduced solely to provide a simple and effective way for transferring the desired measurement locations from design to a mask shop. Therefore, we use the new CATS features that allow highly automated and flexible off-line preparation of measurement jobs. On the KLA-Tencor 8250-R CD-SEM we furthermore utilize its capability of converting CATS output files into fully functional SEM measurement jobs with large numbers of sites and multiple steps of pattern recognition. A comparison of results obtained with the CATS jobs with those of native SEM jobs proves the consistency of data.

  9. Study of the impact of automation on productivity in bus-maintenance facilities. Final report

    SciTech Connect

    Sumanth, D.J.; Weiss, H.J.; Adya, B.

    1988-12-01

    Whether or not the various types of automation and new technologies introduced in a bus-transit system really have an impact on productivity is the question addressed in the study. The report describes a new procedure of productivity measurement and evaluation for a county-transit system and provides an objective perspective on the impact of automation on productivity in bus maintenance facilities. The research objectives were: to study the impact of automation on total productivity in transit maintenance facilities; to develop and apply a methodology for measuring the total productivity of a Floridian transit maintenance facility (Bradenton-Manatee County bus maintenance facility which has been introducing automation since 1983); and to develop a practical step-by-step implementation scheme for the total productivity-based productivity measurement system that any bus manager can use. All 3 objectives were successfully accomplished.

  10. Automated production of plant-based vaccines and pharmaceuticals.

    PubMed

    Wirz, Holger; Sauer-Budge, Alexis F; Briggs, John; Sharpe, Aaron; Shu, Sudong; Sharon, Andre

    2012-12-01

    A fully automated "factory" was developed that uses tobacco plants to produce large quantities of vaccines and other therapeutic biologics within weeks. This first-of-a-kind factory takes advantage of a plant viral vector technology to produce specific proteins within the leaves of rapidly growing plant biomass. The factory's custom-designed robotic machines plant seeds, nurture the growing plants, introduce a viral vector that directs the plant to produce a target protein, and harvest the biomass once the target protein has accumulated in the plants-all in compliance with Food and Drug Administration (FDA) guidelines (e.g., current Good Manufacturing Practices). The factory was designed to be time, cost, and space efficient. The plants are grown in custom multiplant trays. Robots ride up and down a track, servicing the plants and delivering the trays from the lighted, irrigated growth modules to each processing station as needed. Using preprogrammed robots and processing equipment eliminates the need for human contact, preventing potential contamination of the process and economizing the operation. To quickly produce large quantities of protein-based medicines, we transformed a laboratory-based biological process and scaled it into an industrial process. This enables quick, safe, and cost-effective vaccine production that would be required in case of a pandemic. PMID:23015521

  11. Comparison of Automated Treponemal and Nontreponemal Test Algorithms as First-Line Syphilis Screening Assays

    PubMed Central

    Chung, Jae-Woo; Park, Seong Yeon; Chae, Seok Lae

    2016-01-01

    Background Automated Mediace Treponema pallidum latex agglutination (TPLA) and Mediace rapid plasma reagin (RPR) assays are used by many laboratories for syphilis diagnosis. This study compared the results of the traditional syphilis screening algorithm and a reverse algorithm using automated Mediace RPR or Mediace TPLA as first-line screening assays in subjects undergoing a health checkup. Methods Samples from 24,681 persons were included in this study. We routinely performed Mediace RPR and Mediace TPLA simultaneously. Results were analyzed according to both the traditional algorithm and reverse algorithm. Samples with discordant results on the reverse algorithm (e.g., positive Mediace TPLA, negative Mediace RPR) were tested with Treponema pallidum particle agglutination (TPPA). Results Among the 24,681 samples, 30 (0.1%) were found positive by traditional screening, and 190 (0.8%) by reverse screening. The identified syphilis rate and overall false-positive rate according to the traditional algorithm were lower than those according to the reverse algorithm (0.07% and 0.05% vs. 0.64% and 0.13%, respectively). A total of 173 discordant samples were tested with TPPA by using the reverse algorithm, of which 140 (80.9%) were TPPA positive. Conclusions Despite the increased false-positive results in populations with a low prevalence of syphilis, the reverse algorithm detected 140 samples with treponemal antibody that went undetected by the traditional algorithm. The reverse algorithm using Mediace TPLA as a screening test is more sensitive for the detection of syphilis. PMID:26522755

  12. Production and quality assurance automation in the Goddard Space Flight Center Flight Dynamics Facility

    NASA Technical Reports Server (NTRS)

    Chapman, K. B.; Cox, C. M.; Thomas, C. W.; Cuevas, O. O.; Beckman, R. M.

    1994-01-01

    The Flight Dynamics Facility (FDF) at the NASA Goddard Space Flight Center (GSFC) generates numerous products for NASA-supported spacecraft, including the Tracking and Data Relay Satellites (TDRS's), the Hubble Space Telescope (HST), the Extreme Ultraviolet Explorer (EUVE), and the space shuttle. These products include orbit determination data, acquisition data, event scheduling data, and attitude data. In most cases, product generation involves repetitive execution of many programs. The increasing number of missions supported by the FDF has necessitated the use of automated systems to schedule, execute, and quality assure these products. This automation allows the delivery of accurate products in a timely and cost-efficient manner. To be effective, these systems must automate as many repetitive operations as possible and must be flexible enough to meet changing support requirements. The FDF Orbit Determination Task (ODT) has implemented several systems that automate product generation and quality assurance (QA). These systems include the Orbit Production Automation System (OPAS), the New Enhanced Operations Log (NEOLOG), and the Quality Assurance Automation Software (QA Tool). Implementation of these systems has resulted in a significant reduction in required manpower, elimination of shift work and most weekend support, and improved support quality, while incurring minimal development cost. This paper will present an overview of the concepts used and experiences gained from the implementation of these automation systems.

  13. On-line tritium production monitor

    DOEpatents

    Mihalczo, John T.

    1993-01-01

    A scintillation optical fiber system for the on-line monitoring of nuclear reactions in an event-by-event manner is described. In the measurement of tritium production one or more optical fibers are coated with enriched .sup.6 Li and connected to standard scintillation counter circuitry. A neutron generated .sup.6 Li(n )T reaction occurs in the coated surface of .sup.6 Li-coated fiber to produce energetic alpha and triton particles one of which enters the optical fiber and scintillates light through the fiber to the counting circuit. The coated optical fibers can be provided with position sensitivity by placing a mirror at the free end of the fibers or by using pulse counting circuits at both ends of the fibers.

  14. On-line tritium production monitor

    DOEpatents

    Mihalczo, J.T.

    1993-11-23

    A scintillation optical fiber system for the on-line monitoring of nuclear reactions in an event-by-event manner is described. In the measurement of tritium production one or more optical fibers are coated with enriched {sup 6}Li and connected to standard scintillation counter circuitry. A neutron generated {sup 6}Li(n)T reaction occurs in the coated surface of {sup 6}Li-coated fiber to produce energetic alpha and triton particles one of which enters the optical fiber and scintillates light through the fiber to the counting circuit. The coated optical fibers can be provided with position sensitivity by placing a mirror at the free end of the fibers or by using pulse counting circuits at both ends of the fibers. 5 figures.

  15. “Development of an Automated On-line Electrochemical Chlorite Ion Sensor”

    PubMed Central

    Myers, John N.; Steinecker, William H.; Sandlin, Zechariah D.; Cox, James A.; Gordon, Gilbert; Pacey, Gilbert E.

    2012-01-01

    A sensor system for the automatic, in-line, determination of chlorite ion is reported. Electroanalytical measurements were performed in electrolyte-free liquids by using an electrochemical probe (EC), which enables in-line detection in high-resistance media such as disinfected water. Cyclic voltammetry scan rate studies suggest that the current arising from the oxidation of chlorite ion at an EC probe is mass-transfer limited. By coupling FIA with an EC probe amperometric cell, automated analysis was achieved. This sensor is intended to fulfill the daily monitoring requirements of the EPA DBP regulations for chlorite ion. Detection limits of 0.02-0.13 mg/L were attained, which is about one order of magnitude below the MRDL. The sensor showed no faradaic signal for perchlorate, chlorate, or nitrate. The lifetime and stability of the sensor were investigated by measuring calibration curves over time under constant-flow conditions. Detection limits of <0.1 mg/L were repeatedly achieved over a period of three weeks. PMID:22608440

  16. SkyLine and SkyGas: Novel automated technologies for automatic GHG flux measurements

    NASA Astrophysics Data System (ADS)

    Ineson, Philip; Stockdale, James

    2014-05-01

    1. Concerns for the future of the Earth's climate centre around the anthropogenically-driven continuing increases in atmospheric concentrations of the major 'greenhouse gases' (GHGs) which include CO2, CH4 and N2O. A major component of the global budgets for all three of these gases is the flux between the atmosphere and terrestrial ecosystems. 2. Currently, these fluxes are poorly quantified, largely due to technical limitations associated with making these flux measurements. Whilst eddy covariance systems have greatly improved such measurements at the ecosystem scale, flux measurements at the plot scale are commonly made using labour intensive traditional 'cover box' approaches; technical limitations have frequently been a bottle-neck in producing adequate and appropriate GHG flux data necessary for making land management decisions. For example, there are almost no night time flux data for N2O fluxes, and frequently such data are only measured over bare soil patches. 3. We have been addressing the design of novel field equipment for the automation of GHG flux measurements at the chamber and plot scale and will present here some of the technical solutions we have developed. These solutions include the development of the SkyLine and SkyGas approaches which resolve many of the common problems associated with making high frequency, sufficiently replicated GHG flux measurements under field conditions. 4. Unlike most other automated systems, these technologies 'fly' a single chamber to the measurement site, rather than have multiple replicated chambers and analysers. We will present data showing how such systems can deliver high time and spatial resolution flux data, with a minimum of operator intervention and, potentially, at relatively low per plot cost. We will also show how such measurements can be extended to monitoring fluxes from freshwater features in the landscape.

  17. CERES AuTomAted job Loading SYSTem (CATALYST): An automated workflow manager for satellite data production

    NASA Astrophysics Data System (ADS)

    Gleason, J. L.; Hillyer, T. N.; Wilkins, J.

    2012-12-01

    The CERES Science Team integrates data from 5 CERES instruments onboard the Terra, Aqua and NPP missions. The processing chain fuses CERES observations with data from 19 other unique sources. The addition of CERES Flight Model 5 (FM5) onboard NPP, coupled with ground processing system upgrades further emphasizes the need for an automated job-submission utility to manage multiple processing streams concurrently. The operator-driven, legacy-processing approach relied on manually staging data from magnetic tape to limited spinning disk attached to a shared memory architecture system. The migration of CERES production code to a distributed, cluster computing environment with approximately one petabyte of spinning disk containing all precursor input data products facilitates the development of a CERES-specific, automated workflow manager. In the cluster environment, I/O is the primary system resource in contention across jobs. Therefore, system load can be maximized with a throttling workload manager. This poster discusses a Java and Perl implementation of an automated job management tool tailored for CERES processing.

  18. Line Pilots' Attitudes about and Experience with Flight Deck Automation: Results of an International Survey and Proposed Guidelines

    NASA Technical Reports Server (NTRS)

    Rudisill, Marianne

    1995-01-01

    A survey of line pilots' attitudes about flight deck automation was conducted by the Royal Air Force Institute of Aviation Medicine (RAF IAM, Farnborough, UK) under the sponsorship of the United Kingdom s Civil Aviation Authority and in cooperation with IATA (the International Air Transport Association). Survey freehand comments given by pilots operating 13 types of commercial transports across five manufacturers (Airbus, Boeing, British Aerospace, Lockheed, and McDonnell-Douglas) and 57 air carriers/organizations were analyzed by NASA. These data provide a "lessons learned" knowledge base which may be used for the definition of guidelines for flight deck automation and its associated crew interface within the High Speed Research Program. The aircraft chosen for analysis represented a progression of levels of automation sophistication and complexity, from "Basic" types (e.g., B727, DC9), through "Transition" types (e.g., A300, Concorde), to two levels of glass cockpits (e.g., Glass 1: e.g., A310; Glass 2: e.g., B747-400). This paper reports the results of analyses of comments from pilots flying commercial transport types having the highest level of automation sophistication (B757/B767, B747-400, and A320). Comments were decomposed into five categories relating to: (1) general observations with regard to flight deck automation; comments concerning the (2) design and (3) crew understanding of automation and the crew interface; (4) crew operations with automation; and (5) personal factors affecting crew/automation interaction. The goal of these analyses is to contribute to the definition of guidelines which may be used during design of future aircraft flight decks.

  19. Process development for automated solar cell and module production. Task 4: Automated array assembly

    NASA Technical Reports Server (NTRS)

    Hagerty, J. J.

    1981-01-01

    The Unimate robot was programmed for the final 35 cell pattern to be used in the fabrication of the deliverable modules. Mechanical construction of the Automated Lamination Station and Final Assembly Station were completed on schedule. All final wiring and interconnect cables were also completed and the first operational testing began. The final controlling program was written. A local fabricator was contracted to produce the glass reinforced concrete panels to be used for testing and deliverables. A video tape showing all three stations in operation was produced.

  20. Harnessing natural product assembly lines: structure, promiscuity, and engineering.

    PubMed

    Ladner, Christopher C; Williams, Gavin J

    2016-03-01

    Many therapeutically relevant natural products are biosynthesized by the action of giant mega-enzyme assembly lines. By leveraging the specificity, promiscuity, and modularity of assembly lines, a variety of strategies has been developed that enables the biosynthesis of modified natural products. This review briefly summarizes recent structural advances related to natural product assembly lines, discusses chemical approaches to probing assembly line structures in the absence of traditional biophysical data, and surveys efforts that harness the inherent or engineered promiscuity of assembly lines for the synthesis of non-natural polyketides and non-ribosomal peptide analogues. PMID:26527577

  1. Microsoft Business Solutions-Axapta as a basis for automated monitoring of high technology products competitiveness

    NASA Astrophysics Data System (ADS)

    Tashchiyan, G. O.; Sushko, A. V.; Grichin, S. V.

    2015-09-01

    One of the conditions of normal performance of the Russian economy is the problem of high technology products competitiveness. Different tools of these products estimation are used nowadays, one of them is automated monitoring of the high technology products in mechanical engineering. This system is developed on the basis of “Innovator" software integrated in Microsoft Business Solutions-Axapta.

  2. Automation and integration of multiplexed on-line sample preparation with capillary electrophoresis for DNA sequencing

    SciTech Connect

    Tan, H.

    1999-03-31

    The purpose of this research is to develop a multiplexed sample processing system in conjunction with multiplexed capillary electrophoresis for high-throughput DNA sequencing. The concept from DNA template to called bases was first demonstrated with a manually operated single capillary system. Later, an automated microfluidic system with 8 channels based on the same principle was successfully constructed. The instrument automatically processes 8 templates through reaction, purification, denaturation, pre-concentration, injection, separation and detection in a parallel fashion. A multiplexed freeze/thaw switching principle and a distribution network were implemented to manage flow direction and sample transportation. Dye-labeled terminator cycle-sequencing reactions are performed in an 8-capillary array in a hot air thermal cycler. Subsequently, the sequencing ladders are directly loaded into a corresponding size-exclusion chromatographic column operated at {approximately} 60 C for purification. On-line denaturation and stacking injection for capillary electrophoresis is simultaneously accomplished at a cross assembly set at {approximately} 70 C. Not only the separation capillary array but also the reaction capillary array and purification columns can be regenerated after every run. DNA sequencing data from this system allow base calling up to 460 bases with accuracy of 98%.

  3. An Automated HIV-1 Env-Pseudotyped Virus Production for Global HIV Vaccine Trials

    PubMed Central

    Fuss, Martina; Mazzotta, Angela S.; Sarzotti-Kelsoe, Marcella; Ozaki, Daniel A.; Montefiori, David C.; von Briesen, Hagen; Zimmermann, Heiko; Meyerhans, Andreas

    2012-01-01

    Background Infections with HIV still represent a major human health problem worldwide and a vaccine is the only long-term option to fight efficiently against this virus. Standardized assessments of HIV-specific immune responses in vaccine trials are essential for prioritizing vaccine candidates in preclinical and clinical stages of development. With respect to neutralizing antibodies, assays with HIV-1 Env-pseudotyped viruses are a high priority. To cover the increasing demands of HIV pseudoviruses, a complete cell culture and transfection automation system has been developed. Methodology/Principal Findings The automation system for HIV pseudovirus production comprises a modified Tecan-based Cellerity system. It covers an area of 5×3 meters and includes a robot platform, a cell counting machine, a CO2 incubator for cell cultivation and a media refrigerator. The processes for cell handling, transfection and pseudovirus production have been implemented according to manual standard operating procedures and are controlled and scheduled autonomously by the system. The system is housed in a biosafety level II cabinet that guarantees protection of personnel, environment and the product. HIV pseudovirus stocks in a scale from 140 ml to 1000 ml have been produced on the automated system. Parallel manual production of HIV pseudoviruses and comparisons (bridging assays) confirmed that the automated produced pseudoviruses were of equivalent quality as those produced manually. In addition, the automated method was fully validated according to Good Clinical Laboratory Practice (GCLP) guidelines, including the validation parameters accuracy, precision, robustness and specificity. Conclusions An automated HIV pseudovirus production system has been successfully established. It allows the high quality production of HIV pseudoviruses under GCLP conditions. In its present form, the installed module enables the production of 1000 ml of virus-containing cell culture supernatant per

  4. A sensor skid for precise 3D modeling of production lines

    NASA Astrophysics Data System (ADS)

    Elseberg, J.; Borrmann, D.; Schauer, J.; Nüchter, A.; Koriath, D.; Rautenberg, U.

    2014-05-01

    Motivated by the increasing need of rapid characterization of environments in 3D, we designed and built a sensor skid that automates the work of an operator of terrestrial laser scanners. The system combines terrestrial laser scanning with kinematic laser scanning and uses a novel semi-rigid SLAMmethod. It enables us to digitize factory environments without the need to stop production. The acquired 3D point clouds are precise and suitable to detect objects that collide with items moved along the production line.

  5. Production system chunking in SOAR: Case studies in automated learning

    NASA Technical Reports Server (NTRS)

    Allen, Robert

    1989-01-01

    A preliminary study of SOAR, a general intelligent architecture for automated problem solving and learning, is presented. The underlying principles of universal subgoaling and chunking were applied to a simple, yet representative, problem in artificial intelligence. A number of problem space representations were examined and compared. It is concluded that learning is an inherent and beneficial aspect of problem solving. Additional studies are suggested in domains relevant to mission planning and to SOAR itself.

  6. Survey of Product-line Verification and Validation Techniques

    NASA Technical Reports Server (NTRS)

    Lutz, Robyn

    2007-01-01

    This report presents the results from the first task of the SARP Center Initiative, 'Product Line Verification of Safety-Critical Software.' Task 1 is a literature survey of available techniques for product line verification and validation. Section 1 of the report provides an introduction to product lines and motivates the survey of verification techniques. It describes what is reused in product-line engineering and explains the goal of verifiable conformance of the developed system to its product-line specifications. Section 2 of the report describes six lifecycle steps in product-line verification and validation. This description is based on, and refers to, the best practices extracted from the readings. It ends with a list of verification challenges for NASA product lines (2.7) and verification enablers for NASA product lines (2.8) derived from the survey. Section 3 provides resource lists of related conferences, workshops, industrial and defense industry experiences and case studies of product lines, and academic/industrial consortiums. Section 4 is a bibliography of papers and tutorials with annotated entries for relevant papers not previously discussed in sections 2 or 3.

  7. Technology Management within Product Lines in High Technology Markets

    ERIC Educational Resources Information Center

    Sarangee, Kumar R.

    2009-01-01

    Understanding the nuances of product line management has been of great interest to business scholars and practitioners. This assumes greater significance for firms conducting business in technologically dynamic industries, where they face certain challenges regarding the management of multiple, overlapping technologies within their product lines.…

  8. Automated Performance Tracking and Productivity Improvement Project. Annual Report: Innovations in Protective Services.

    ERIC Educational Resources Information Center

    Dennis-Small, Lucretia

    Findings are reported of a process evaluation of the Automated Performance Tracking and Productivity Improvement Project, a project developed to enhance the productivity and efficiency of child protective services (CPS) staff and to enable Region 10 of the Texas Department of Human Services to better meet federal and state performance guidelines.…

  9. Informational needs for a product line management system.

    PubMed

    Alfirevic, J; Kroman, B; Ruflin, P

    1987-03-01

    Changing to a product line management structure requires a total dedicated effort from hospital management. The information systems support requirements are diverse and often call for both the further integration of existing capabilities as well as the development of new ones. The reporting structure must address the profit and quality management objectives assigned to both product line and functional managers. A thorough planning and evaluation process that recognizes the institution's unique objectives is the key to successful implementation. This article is the final in a series on product line management in health care. The previous two articles examined the concepts and evolution of product line management and the alternative product line organizational models. PMID:10280835

  10. Automation in sludge dewatering by novel on-line characterisation of flocculation.

    PubMed

    Sievers, M; Schroeder, C; Bormann, H; Onyeche, T I; Schlaefer, O; Schaefer, S

    2003-01-01

    A novel on-line method and system for characterisation of sludge flocs in view of improving sludge dewatering has been developed. The characterisation of sludge flocs was carried out after the conditioning or the flocculation process. The system uses a conventional CCD-line scan camera providing an on-line monitoring of the relative floc size distribution by image processing procedures. The image processing procedure has been re-evaluated and adapted to the practical dewatering results, obtained from a chamber filter press of 250 x 250 mm size. A good correlation between the calculated sensor signal and the sludge dewaterability of digested sludge in terms of the up-concentration factor was found. Although different sludge compositions and flocculation systems have been tested within the whole experimental period of six months, a good reproducibility of this correlation was also found. A well balanced floc size distribution is necessary showing that not too many but still some fine flocs and also not too large but compact flocs lead to improved dewaterability. This has been illustrated by an extended range of floc size measurements ranging between 50 microm and 29 mm. The conditioning monitoring system would be suitable for the control of production of good size-balanced flocs to compensate fluctuations in sludge characteristics of the sludge to be conditioned. PMID:12636075

  11. A Physics-based Automated Technique for the Detection of Field Line Resonance Frequency in Ground Magnetometer Data

    NASA Astrophysics Data System (ADS)

    Boudouridis, A.; Zesta, E.; Moldwin, M.

    2015-12-01

    The accurate determination of the Field Line Resonance (FLR) frequency of a resonating geomagnetic field line is necessary for the remote monitoring of the plasmaspheric mass density during geomagnetic storms and quiet times alike. Under certain assumptions the plasmaspheric mass density at the equator is inversely proportional to the square of the FLR frequency. The most common techniques to determine the FLR frequency from ground magnetometer measurements are the amplitude ratio and phase difference techniques, both based on geomagnetic field measurements at two latitudinally separated ground stations. Previously developed automated techniques have used statistical methods to pinpoint the FLR frequency using the amplitude ratio and phase difference calculations. We now introduce a physics-based automated technique that can reproduce the resonant wave characteristics from the two ground station data, and from those determine the FLR frequency. The advantage of the new technique, besides moving away from ambiguous statistical manipulations of the ground data, is the estimation of physically determined errors of the FLR frequency, which can yield physically determined errors of the equatorial plasmaspheric mass density. We present preliminary results of the new technique calculations, and test it using data from the new Inner-Magnetospheric Array for Geospace Science (iMAGS) ground magnetometer chain along the coast of Chile and the east coast of the United States. We compare the results with the results of previously published statistical automated techniques.

  12. Reliability and maintainability analysis of bread production line.

    PubMed

    Tsarouhas, Panagiotis H; Arvanitoyannis, Ioannis S

    2010-04-01

    The statistical analysis of the bread production line of the failure and repair data at machine and line levels was displayed. The experiment covers a period of twenty-five months. The best fit of the failure data between the common theoretical distributions was found and its parameters were computed. The reliability and hazard rate modes for all machines and the entire production line were calculated as well. The models could prove to be a useful tool to assess the current conditions, and to predict the reliability for upgrading the maintenance policy of the production line. It was pointed out that (a) the availability of the bread production line is 90.74% and went down to 86.76% because the equipment's failures cause an additional production gap in the line, (b) the 53.5% of all failures occurred at the bread machine, cooling tower machine, and volumetric-divider machine, and (c) the machines of the bread production line that displayed increasing hazard rate functions were identified. This analysis will be very useful in terms of identifying the occurring and latent problems in manufacturing process of bread and improve it. PMID:20301018

  13. Capillary electrophoresis for automated on-line monitoring of suspension cultures: Correlating cell density, nutrients and metabolites in near real-time.

    PubMed

    Alhusban, Ala A; Breadmore, Michael C; Gueven, Nuri; Guijt, Rosanne M

    2016-05-12

    Increasingly stringent demands on the production of biopharmaceuticals demand monitoring of process parameters that impact on their quality. We developed an automated platform for on-line, near real-time monitoring of suspension cultures by integrating microfluidic components for cell counting and filtration with a high-resolution separation technique. This enabled the correlation of the growth of a human lymphocyte cell line with changes in the essential metabolic markers, glucose, glutamine, leucine/isoleucine and lactate, determined by Sequential Injection-Capillary Electrophoresis (SI-CE). Using 8.1 mL of media (41 μL per run), the metabolic status and cell density were recorded every 30 min over 4 days. The presented platform is flexible, simple and automated and allows for fast, robust and sensitive analysis with low sample consumption and high sample throughput. It is compatible with up- and out-scaling, and as such provides a promising new solution to meet the future demands in process monitoring in the biopharmaceutical industry. PMID:27114228

  14. Research on flexible automation and robotics for plant production at Rutgers University.

    PubMed

    Ting, K C; Ling, P P; Giacomelli, G A

    1996-01-01

    This is an overview of research activities in the areas of flexible automation and robotics (FAR) within controlled environment plant production systems (CEPPS) in the Department of Bioresource Engineering, Rutgers University. In the past thirty years, our CEPPS research has dealt with the topics including structures and energy, environmental monitoring and control, plant growing systems, operations research and decision support systems, flexible automation and robotics, and impact to natural (i.e. surrounding) environment. Computer and modeling/simulation techniques have been utilized extensively. Mechanized systems have been developed to substitute human's physical labor and maintain uniformity in production. Automation research has been directed towards adding, to the mechanized systems, the capabilities of perception, reasoning, communication, and task planning. Computers, because of their programmability, provide flexibility to automated systems, when incorporated with generic hardware devices. Robots are ideal hardware tools to be employed in flexible automation systems. Some technologies developed in our CEPPS research may be readily adaptable to Closed Bioregenerative Life Support Systems (CBLSS). PMID:11538960

  15. Production of an Automated Lesson for Use on the Audio Visual Response System.

    ERIC Educational Resources Information Center

    Rowse, Nancy

    The development, production, and evaluation of an automated lesson for use on the Audio Visual Response System (AVR System 400) is described in this paper. Establishment of learning objectives was the central focus of the lesson development process, followed by script development and the planning and design of illustrative materials. These…

  16. Evaluation of different lactation curve models fitted for milk viscosity recorded by an automated on-line California Mastitis Test.

    PubMed

    Neitzel, Anne-Christin; Stamer, Eckhard; Junge, Wolfgang; Thaller, Georg

    2015-05-01

    Laboratory somatic cell count (LSCC) records are usually recorded monthly and provide an important information source for breeding and herd management. Daily milk viscosity detection in composite milking (expressed as drain time) with an automated on-line California Mastitis Test (CMT) could serve immediately as an early predictor of udder diseases and might be used as a selection criterion to improve udder health. The aim of the present study was to clarify the relationship between the well-established LSCS and the new trait,'drain time', and to estimate their correlations to important production traits. Data were recorded on the dairy research farm Karkendamm in Germany. Viscosity sensors were installed on every fourth milking stall in the rotary parlour to measure daily drain time records. Weekly LSCC and milk composition data were available. Two data sets were created containing records of 187,692 milkings from 320 cows (D1) and 25,887 drain time records from 311 cows (D2). Different fixed effect models, describing the log-transformed drain time (logDT), were fitted to achieve applicable models for further analysis. Lactation curves were modelled with standard parametric functions (Ali and Schaeffer, Legendre polynomials of second and third degree) of days in milk (DIM). Random regression models were further applied to estimate the correlations between cow effects between logDT and LSCS with further important production traits. LogDT and LSCS were strongest correlated in mid-lactation (r = 0.78). Correlations between logDT and production traits were low to medium. Highest correlations were reached in late lactation between logDT and milk yield (r = -0.31), between logDT and protein content (r = 0.30) and in early as well as in late lactation between logDT and lactose content (r = -0.28). The results of the present study show that the drain time could be used as a new trait for daily mastitis control. PMID:25731191

  17. Tempest gas turbine extends EGT product line

    SciTech Connect

    Chellini, R.

    1995-07-01

    With the introduction of the 7.8 MW (mechanical output) Tempest gas turbine, ECT has extended the company`s line of its small industrial turbines. The new Tempest machine, featuring a 7.5 MW electric output and a 33% thermal efficiency, ranks above the company`s single-shaft Typhoon gas turbine, rated 3.2 and 4.9 MW, and the 6.3 MW Tornado gas turbine. All three machines are well-suited for use in combined heat and power (CHP) plants, as demonstrated by the fact that close to 50% of the 150 Typhoon units sold are for CHP applications. This experience has induced EGT, of Lincoln, England, to announce the introduction of the new gas turbine prior to completion of the testing program. The present single-shaft machine is expected to be used mainly for industrial trial cogeneration. This market segment, covering the needs of paper mills, hospitals, chemical plants, ceramic industry, etc., is a typical local market. Cogeneration plants are engineered according to local needs and have to be assisted by local organizations. For this reason, to efficiently cover the world market, EGT has selected a number of associates that will receive from Lincoln completely engineered machine packages and will engineer the cogeneration system according to custom requirements. These partners will also assist the customer and dispose locally of the spares required for maintenance operations.

  18. Implementation of automated macro after develop inspection in a production lithography process

    NASA Astrophysics Data System (ADS)

    Yanof, Arnold W.; Plachecki, Vincent E.; Fischer, Frank W.; Cusacovich, Marcelo; Nelson, Chris; Merrill, Mark A.

    2000-06-01

    impossibility of accurate classification and recording of defect types, locations, and layer of occurrence. In this paper, we discuss a pilot implementation of an automated macro inspection system at Motorola, Inc., which has enabled the early detection and containment of significant photolithography defects. We show a variety of different types of defects that have been effectively detected and identified by this system during production usage. We introduce a methodology for determining the automated tool's ability to discriminate between the defect signal and process noise. We indicate the potential for defect database analysis, and identification of maverick product. Based upon the pilot experience, we discuss the parameters of a cost/benefit analysis of full implementation. The costs involve tool cost, additional wafer dispositions, and the engineering costs of recipe management. The most tangible measurable benefit is the saved revenue of scrapped wafers. An analysis of risk also shows a major reduction due to improved detection, as well as reduced occurrence because of better containment. This reduction of risk extends both to the customer -- in terms of field failures, OTD, maverick product -- as well as to the production facility -- in terms of major scrap incidents, forced inking at probe, redo, and containment.

  19. Automated Production of High Rep Rate Foam Targets

    NASA Astrophysics Data System (ADS)

    Hall, F.; Spindloe, C.; Haddock, D.; Tolley, M.; Nazarov, W.

    2016-04-01

    Manufacturing low density targets in the numbers needed for high rep rate experiments is highly challenging. This report summarises advances from manual production to semiautomated and the improvements that follow both in terms of production time and target uniformity. The production process is described and shown to be improved by the integration of an xyz robot with dispensing capabilities. Results are obtained from manual and semiautomated production runs and compared. The variance in the foam thickness is reduced significantly which should decrease experimental variation due to target parameters and could allow for whole batches to be characterised by the measurement of a few samples. The work applies to both foil backed and free standing foam targets.

  20. Automation and quality assurance of the production cycle

    NASA Astrophysics Data System (ADS)

    Hajdu, L.; Didenko, L.; Lauret, J.

    2010-04-01

    Processing datasets on the order of tens of terabytes is an onerous task, faced by production coordinators everywhere. Users solicit data productions and, especially for simulation data, the vast amount of parameters (and sometime incomplete requests) point at the need for a tracking, control and archiving all requests made so a coordinated handling could be made by the production team. With the advent of grid computing the parallel processing power has increased but traceability has also become increasing problematic due to the heterogeneous nature of Grids. Any one of a number of components may fail invalidating the job or execution flow in various stages of completion and re-submission of a few of the multitude of jobs (keeping the entire dataset production consistency) a difficult and tedious process. From the definition of the workflow to its execution, there is a strong need for validation, tracking, monitoring and reporting of problems. To ease the process of requesting production workflow, STAR has implemented several components addressing the full workflow consistency. A Web based online submission request module, implemented using Drupal's Content Management System API, enforces ahead that all parameters are described in advance in a uniform fashion. Upon submission, all jobs are independently tracked and (sometime experiment-specific) discrepancies are detected and recorded providing detailed information on where/how/when the job failed. Aggregate information on success and failure are also provided in near real-time.

  1. Automated tuning of a CO{sub 2} laser to a required oscillation line without a spectral instrument

    SciTech Connect

    Petukhov, V O; Gorobets, V A

    2005-02-28

    The method is proposed for tuning CO{sub 2} laser to a required oscillation line without a spectral instrument based on the coincidence of transition frequencies belonging to different vibrational-rotational bands of the CO{sub 2} molecule. This coincidence leads to the anomaly in the gain distribution over rotational sublevels, thereby affecting the laser output parameters. The method was successfully applied to a completely automated low-pressure, longitudinal-discharge cw CO{sub 2} laser and a pulsed TEA CO{sub 2} laser. (control of laser radiation parameters)

  2. Vaccine production: upstream processing with adherent or suspension cell lines.

    PubMed

    Genzel, Yvonne; Rödig, Jana; Rapp, Erdmann; Reichl, Udo

    2014-01-01

    The production of viral vaccines in cell culture can be accomplished with primary, diploid, or continuous (transformed) cell lines. Each cell line, each virus type, and each vaccine preparation require the specific design of upstream and downstream processing. Media have to be selected as well as production vessels, cultivation conditions, and modes of operation. Many viruses only replicate to high titers in adherently growing cells, but similar to processes established for recombinant protein production, an increasing number of suspension cell lines is being evaluated for future use. Here, we describe key issues to be considered for the establishment of large-scale virus production in bioreactors. As an example upstream processing of cell culture-derived influenza virus production is described in more detail for adherently growing and for suspension cells. In particular, use of serum-containing, serum-free, and chemically defined media as well as choice of cultivation vessel are considered. PMID:24297427

  3. Automated segmentation and feature extraction of product inspection items

    NASA Astrophysics Data System (ADS)

    Talukder, Ashit; Casasent, David P.

    1997-03-01

    X-ray film and linescan images of pistachio nuts on conveyor trays for product inspection are considered. The final objective is the categorization of pistachios into good, blemished and infested nuts. A crucial step before classification is the separation of touching products and the extraction of features essential for classification. This paper addresses new detection and segmentation algorithms to isolate touching or overlapping items. These algorithms employ a new filter, a new watershed algorithm, and morphological processing to produce nutmeat-only images. Tests on a large database of x-ray film and real-time x-ray linescan images of around 2900 small, medium and large nuts showed excellent segmentation results. A new technique to detect and segment dark regions in nutmeat images is also presented and tested on approximately 300 x-ray film and approximately 300 real-time linescan x-ray images with 95-97 percent detection and correct segmentation. New algorithms are described that determine nutmeat fill ratio and locate splits in nutmeat. The techniques formulated in this paper are of general use in many different product inspection and computer vision problems.

  4. Automated breeder fuel fabrication

    SciTech Connect

    Goldmann, L.H.; Frederickson, J.R.

    1983-09-01

    The objective of the Secure Automated Fabrication (SAF) Project is to develop remotely operated equipment for the processing and manufacturing of breeder reactor fuel pins. The SAF line will be installed in the Fuels and Materials Examination Facility (FMEF). The FMEF is presently under construction at the Department of Energy's (DOE) Hanford site near Richland, Washington, and is operated by the Westinghouse Hanford Company (WHC). The fabrication and support systems of the SAF line are designed for computer-controlled operation from a centralized control room. Remote and automated fuel fabriction operations will result in: reduced radiation exposure to workers; enhanced safeguards; improved product quality; near real-time accountability, and increased productivity. The present schedule calls for installation of SAF line equipment in the FMEF beginning in 1984, with qualifying runs starting in 1986 and production commencing in 1987. 5 figures.

  5. A fully automated system for analysis of pesticides in water: on-line extraction followed by liquid chromatography-tandem photodiode array/postcolumn derivatization/fluorescence detection.

    PubMed

    Patsias, J; Papadopoulou-Mourkidou, E

    1999-01-01

    A fully automated system for on-line solid phase extraction (SPE) followed by high-performance liquid chromatography (HPLC) with tandem detection with a photodiode array detector and a fluorescence detector (after postcolumn derivatization) was developed for analysis of many chemical classes of pesticides and their major conversion products in aquatic systems. An automated on-line-SPE system (Prospekt) operated with reversed-phase cartridges (PRP-1) extracts analytes from 100 mL acidified (pH = 3) filtered water sample. On-line HPLC analysis is performed with a 15 cm C18 analytical column eluted with a mobile phase of phosphate (pH = 3)-acetonitrile in 25 min linear gradient mode. Solutes are detected by tandem diode array/derivatization/fluorescence detection. The system is controlled and monitored by a single computer operated with Millenium software. Recoveries of most analytes in samples fortified at 1 microgram/L are > 90%, with relative standard deviation values of < 5%. For a few very polar analytes, mostly N-methylcarbamoyloximes (i.e., aldicarb sulfone, methomyl, and oxamyl), recoveries are < 20%. However, for these compounds, as well as for the rest of the N-methylcarbamates except for aldicarb sulfoxide and butoxycarboxim, the limits of detection (LODs) are 0.005-0.05 microgram/L. LODs for aldicarb sulfoxide and butoxycarboxim are 0.2 and 0.1 microgram, respectively. LODs for the rest of the analytes except 4-nitrophenol, bentazone, captan, decamethrin, and MCPA are 0.05-0.1 microgram/L. LODs for the latter compounds are 0.2-1.0 microgram/L. The system can be operated unattended. PMID:10444834

  6. Automated two-step chromatography using an ÄKTA equipped with in-line dilution capability.

    PubMed

    Winters, Dwight; Chu, Carolyn; Walker, Kenneth

    2015-12-11

    There has been a great emphasis on developing higher-throughput protein purification techniques to screen potential human therapeutics faster and more efficiently. Not only is it desirable to have high-throughput purification for initial screens but it is also desirable to efficiently purify selected protein therapeutics in the amounts and purity required for definitive assays. Current automated tandem technologies involve size exclusion as a second step that often fails to generate the required purity, is not robust and can only be operated at a limited scale. We have modified an ÄKTA to enable in-line dilution, assuring that the automated loading of a second column from a first column elution can be modified to a pH and ionic strength which is suitable for binding to the second column. For example, Protein A can be employed as a first step followed by direct loading on to a cation exchange column by conditioning the Protein A elution using the in-line diluter. Using this method as described, up to six samples of 1L each can be purified through two columns without human intervention per day per machine, and the system produces good yields of purified protein over a wide range of loading levels (12-300mg). In addition, the system employs guanidine HCl regeneration, followed by a sodium hydroxide wash between purification runs, minimizing the possibility of carryover contamination. The system is described at the 5mL and the 10mL column sizes; however, it could readily be programed for 100mL columns to enable larger-scale purifications. Using this system to automate two-column purifications minimizes human intervention, increases efficiency and minimizes the risk of human error. PMID:26596873

  7. A gradient-based approach for automated crest-line detection and analysis of sand dune patterns on planetary surfaces

    NASA Astrophysics Data System (ADS)

    Lancaster, N.; LeBlanc, D.; Bebis, G.; Nicolescu, M.

    2015-12-01

    Dune-field patterns are believed to behave as self-organizing systems, but what causes the patterns to form is still poorly understood. The most obvious (and in many cases the most significant) aspect of a dune system is the pattern of dune crest lines. Extracting meaningful features such as crest length, orientation, spacing, bifurcations, and merging of crests from image data can reveal important information about the specific dune-field morphological properties, development, and response to changes in boundary conditions, but manual methods are labor-intensive and time-consuming. We are developing the capability to recognize and characterize patterns of sand dunes on planetary surfaces. Our goal is to develop a robust methodology and the necessary algorithms for automated or semi-automated extraction of dune morphometric information from image data. Our main approach uses image processing methods to extract gradient information from satellite images of dune fields. Typically, the gradients have a dominant magnitude and orientation. In many cases, the images have two major dominant gradient orientations, for the sunny and shaded side of the dunes. A histogram of the gradient orientations is used to determine the dominant orientation. A threshold is applied to the image based on gradient orientations which agree with the dominant orientation. The contours of the binary image can then be used to determine the dune crest-lines, based on pixel intensity values. Once the crest-lines have been extracted, the morphological properties can be computed. We have tested our approach on a variety of images of linear and crescentic (transverse) dunes and compared dune detection algorithms with manually-digitized dune crest lines, achieving true positive values of 0.57-0.99; and false positives values of 0.30-0.67, indicating that out approach is generally robust.

  8. Recombinant protein production from stable mammalian cell lines and pools.

    PubMed

    Hacker, David L; Balasubramanian, Sowmya

    2016-06-01

    We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site-specific recombinases (targeted transgene integration). Each of these methods results in the generation of cell lines with protein yields that are generally superior to those achievable through classical plasmid transfection that depends on the integration of the transfected DNA by non-homologous DNA end-joining. This is the main reason why these techniques can also be used for the generation of stable cell pools, heterogenous populations of recombinant cells generated by gene delivery and genetic selection without resorting to single cell cloning. This allows the time line from gene transfer to protein production to be reduced. PMID:27322762

  9. Software Product Line Engineering Approach for Enhancing Agile Methodologies

    NASA Astrophysics Data System (ADS)

    Martinez, Jabier; Diaz, Jessica; Perez, Jennifer; Garbajosa, Juan

    One of the main principles of Agile methodologies consists in the early and continuous delivery of valuable software by short time-framed iterations. After each iteration, a working product is delivered according to the requirements defined at the beginning of the iteration. Testing tools facilitate the task of checking if the system provides the expected behavior according to the specified requirements. However, since testing tools need to be adapted in order to test new working products in each iteration, a significant effort has to be invested. This work presents a Software Product Line Engineering (SPLE) approach that allows flexibility in the adaption of testing tools with the working products in an iterative way. A case study is also presented using PLUM (Product Line Unified Modeller) as the tool suite for SPL implementation and management.

  10. Verifying Architectural Design Rules of the Flight Software Product Line

    NASA Technical Reports Server (NTRS)

    Ganesan, Dharmalingam; Lindvall, Mikael; Ackermann, Chris; McComas, David; Bartholomew, Maureen

    2009-01-01

    This paper presents experiences of verifying architectural design rules of the NASA Core Flight Software (CFS) product line implementation. The goal of the verification is to check whether the implementation is consistent with the CFS architectural rules derived from the developer's guide. The results indicate that consistency checking helps a) identifying architecturally significant deviations that were eluded during code reviews, b) clarifying the design rules to the team, and c) assessing the overall implementation quality. Furthermore, it helps connecting business goals to architectural principles, and to the implementation. This paper is the first step in the definition of a method for analyzing and evaluating product line implementations from an architecture-centric perspective.

  11. SAGA: A project to automate the management of software production systems

    NASA Technical Reports Server (NTRS)

    Campbell, R. H.; Badger, W.; Beckman, C. S.; Beshers, G.; Hammerslag, D.; Kimball, J.; Kirslis, P. A.; Render, H.; Richards, P.; Terwilliger, R.

    1984-01-01

    The project to automate the management of software production systems is described. The SAGA system is a software environment that is designed to support most of the software development activities that occur in a software lifecycle. The system can be configured to support specific software development applications using given programming languages, tools, and methodologies. Meta-tools are provided to ease configuration. Several major components of the SAGA system are completed to prototype form. The construction methods are described.

  12. Interdisciplinary development of manual and automated product usability assessments for older adults with dementia: lessons learned.

    PubMed

    Boger, Jennifer; Taati, Babak; Mihailidis, Alex

    2016-10-01

    The changes in cognitive abilities that accompany dementia can make it difficult to use everyday products that are required to complete activities of daily living. Products that are inherently more usable for people with dementia could facilitate independent activity completion, thus reducing the need for caregiver assistance. The objectives of this research were to: (1) gain an understanding of how water tap design impacted tap usability and (2) create an automated computerized tool that could assess tap usability. 27 older adults, who ranged from cognitively intact to advanced dementia, completed 1309 trials on five tap designs. Data were manually analyzed to investigate tap usability as well as used to develop an automated usability analysis tool. Researchers collaborated to modify existing techniques and to create novel ones to accomplish both goals. This paper presents lessons learned through the course of this research, which could be applicable in the development of other usability studies, automated vision-based assessments and the development of assistive technologies for cognitively impaired older adults. Collaborative interdisciplinary teamwork, which included older adult with dementia participants, was key to enabling innovative advances that achieved the projects' research goals. Implications for Rehabilitation Products that are implicitly familiar and usable by older adults could foster independent activity completion, potentially reducing reliance on a caregiver. The computer-based automated tool can significantly reduce the time and effort required to perform product usability analysis, making this type of analysis more feasible. Interdisciplinary collaboration can result in a more holistic understanding of assistive technology research challenges and enable innovative solutions. PMID:26135222

  13. TOPS On-Line: Automating the Construction and Maintenance of HTML Pages

    NASA Technical Reports Server (NTRS)

    Jones, Kennie H.

    1994-01-01

    After the Technology Opportunities Showcase (TOPS), in October, 1993, Langley Research Center's (LaRC) Information Systems Division (ISD) accepted the challenge to preserve the investment in information assembled in the TOPS exhibits by establishing a data base. Following the lead of several people at LaRC and others around the world, the HyperText Transport Protocol (HTTP) server and Mosaic were the obvious tools of choice for implementation. Initially, some TOPS exhibitors began the conventional approach of constructing HyperText Markup Language (HTML) pages of their exhibits as input to Mosaic. Considering the number of pages to construct, a better approach was conceived that would automate the construction of pages. This approach allowed completion of the data base construction in a shorter period of time using fewer resources than would have been possible with the conventional approach. It also provided flexibility for the maintenance and enhancement of the data base. Since that time, this approach has been used to automate construction of other HTML data bases. Through these experiences, it is concluded that the most effective use of the HTTP/Mosaic technology will require better tools and techniques for creating, maintaining and managing the HTML pages. The development and use of these tools and techniques are the subject of this document.

  14. Increasing inspection equipment productivity by utilizing factory automation SW on TeraScan 5XX systems

    NASA Astrophysics Data System (ADS)

    Jakubski, Thomas; Piechoncinski, Michal; Moses, Raphael; Bugata, Bharathi; Schmalfuss, Heiko; Köhler, Ines; Lisowski, Jan; Klobes, Jens; Fenske, Robert

    2009-01-01

    Especially for advanced masks the reticle inspection operation is a very significant cost factor, since it is a time consuming process and inspection tools are becoming disproportionately expensive. Analyzing and categorizing historical equipment utilization times of the reticle inspection tools however showed a significant amount of time which can be classified as non productive. In order to reduce the inspection costs the equipment utilization needed to be improved. The main contributors to non productive time were analyzed and several use cases identified, where automation utilizing a SECS1 equipment interface was expected to help to reduce these non productive times. The paper demonstrates how real time access to equipment utilization data can be applied to better control manufacturing resources. Scenarios are presented where remote monitoring and control of the inspection equipment can be used to avoid setup errors or save inspection time by faster response to problem situations. Additionally a solution to the second important need, the maximization of tool utilization in cases where not all of the intended functions are available, is explained. Both the models and the software implementation are briefly explained. For automation of the so called inspection strategy a new approach which allows separation of the business rules from the automation infrastructure was chosen. Initial results of inspection equipment performance data tracked through the SECS interface are shown. Furthermore a system integration overview is presented and examples of how the inspection strategy rules are implemented and managed are given.

  15. Sex differences on a measure of conformity in automated teller machine lines.

    PubMed

    Reysen, Stephen; Reysen, Matthew B

    2004-10-01

    Sex differences in conformity were examined as participants approached two ATMs, one of which was occupied by three confederates and the other immediately available. The number of men and women in the line in front of one of the ATMs was manipulated (3 men or 3 women), and an unobtrusive observer recorded the sex of each participant. The results indicated that women were more likely than men to wait in line to use the ATM regardless of the makeup of the line. Thus, the present study provides evidence in favor of the idea that sex differences in conformity are evident on a common task performed in a natural setting. PMID:15587205

  16. Process development for automated solar-cell and module production. Task 4. Automated array assembly. Quarterly report No. 3

    SciTech Connect

    Hagerty, J. J.; Gifford, M.

    1981-04-15

    The Automated Lamination Station is mechanically complete and is currently undergoing final wiring. The high current driver and isolator boards have been completed and installed, and the main interface board is under construction. The automated vacuum chamber has had a minor redesign to increase stiffness and improve the cover open/close mechanism. Design of the Final Assembly Station has been completed and construction is underway.

  17. Automated Optical Extraction from Line Arrays of the Alignment Between Microfabricated Layers

    NASA Technical Reports Server (NTRS)

    Lieneweg, Udo

    1997-01-01

    Machine reading of layer alignment from line arrays in fully fabricated wafers is demonstrated. Misalignment is calculated from the correlation funcation of optical intensity scans through arrays in the two layers.

  18. [A new automated method to identify emission line star from massive spectra].

    PubMed

    Pan, Jing-Chang; Zhang, Cai-Ming; Wei, Peng; Luo, A-Li; Zhao, Yong-Heng

    2012-06-01

    Stellar spectra are characterized by obvious absorption lines or absorption bands, while those with emission lines are usually special stars such as cataclysmic variable stars (CVs), HerbigAe/Be etc. The further study of this kind of spectra is meaningful. The present paper proposed a new method to identify emission line stars (ELS) spectra automatically. After the continuum normalization is done for the original spectral flux, line detection is made by comparing the normalized flux with the mean and standard deviation of the flux in its neighbor region The results of the experiment on massive spectra from SDSS DR8 indicate that the method can identify ELS spectra completely and accurately. Since no complex transformation and computation are involved in this method, the identifying process is fast and it is ideal for the ELS detection in large sky survey projects like LAMOST and SDSS. PMID:22870668

  19. How a heart network can influence a cardiovascular product line.

    PubMed

    Cotham, P; Gilmer, C

    1993-01-01

    The authors believe that a viable heart network program can give tertiary centers the competitive edge in cardiology marketing, can provide the opportunity to fine-tune cardiovascular product lines and can position hospitals to meet the challenges of the next decade in healthcare. However, success will be determined by an institution's ability to carefully balance cost and quality issues. PMID:10126710

  20. Space Station Freedom automation and robotics: An assessment of the potential for increased productivity

    NASA Technical Reports Server (NTRS)

    Weeks, David J.; Zimmerman, Wayne F.; Swietek, Gregory E.; Reid, David H.; Hoffman, Ronald B.; Stammerjohn, Lambert W., Jr.; Stoney, William; Ghovanlou, Ali H.

    1990-01-01

    This report presents the results of a study performed in support of the Space Station Freedom Advanced Development Program, under the sponsorship of the Space Station Engineering (Code MT), Office of Space Flight. The study consisted of the collection, compilation, and analysis of lessons learned, crew time requirements, and other factors influencing the application of advanced automation and robotics, with emphasis on potential improvements in productivity. The lessons learned data collected were based primarily on Skylab, Spacelab, and other Space Shuttle experiences, consisting principally of interviews with current and former crew members and other NASA personnel with relevant experience. The objectives of this report are to present a summary of this data and its analysis, and to present conclusions regarding promising areas for the application of advanced automation and robotics technology to the Space Station Freedom and the potential benefits in terms of increased productivity. In this study, primary emphasis was placed on advanced automation technology because of its fairly extensive utilization within private industry including the aerospace sector. In contrast, other than the Remote Manipulator System (RMS), there has been relatively limited experience with advanced robotics technology applicable to the Space Station. This report should be used as a guide and is not intended to be used as a substitute for official Astronaut Office crew positions on specific issues.

  1. Cell line profiling to improve monoclonal antibody production.

    PubMed

    Kang, Sohye; Ren, Da; Xiao, Gang; Daris, Kristi; Buck, Lynette; Enyenihi, Atim A; Zubarev, Roman; Bondarenko, Pavel V; Deshpande, Rohini

    2014-04-01

    Mammalian cell culture performance is influenced by both intrinsic (genetic) and extrinsic (media and process) factors. In this study, intrinsic capacity of various monoclonal antibody-producing Chinese Hamster Ovary (CHO) cell lines was compared by exposing them to the same culture condition. Microarray-based transcriptomics and LC-MS/MS shotgun proteomics technologies were utilized to obtain expression landscape of different cell lines. Specific transcripts and proteins correlating with productivity, growth rate and cell size have been identified. The proteomics analysis results showed a strong correlation between the intracellular protein expression levels of the recombinant DHFR and productivity. In contrast, neither the light chain nor the heavy chain of the recombinant monoclonal antibody showed correlation to productivity. Other top ranked proteins which demonstrated positive correlation to productivity included the adaptor protein complex subunits AP3D1and AP2B2, DNA repair protein DDB1 and the ER translocation complex component, SRPR. The subunits of molecular chaperone T-complex protein 1 and the regulator of mitochondrial one-carbon metabolism MTHFD2 showed negative correlation to productivity. The transcriptomics analysis has identified the regulators of calcium signaling, Tmem20 and Rcan1, as the top ranked genes displaying positive and negative correlation to productivity, respectively. For the second part of the study, the principal component analysis (PCA) was generated to view the underlying global structure of the expression data. A clear division and expression polarity was observed between the two distinct clusters of cell lines, independent of link to productivity or any other traits examined. The primary component of the PCA generated from either transcriptomics or proteomics data displayed a strong correlation to cell size and doubling time, while none of the main principal components showed correlation to productivity. Our findings suggest

  2. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris.

    PubMed

    Shah, Kartik A; Clark, John J; Goods, Brittany A; Politano, Timothy J; Mozdzierz, Nicholas J; Zimnisky, Ross M; Leeson, Rachel L; Love, J Christopher; Love, Kerry R

    2015-12-01

    Monoclonal antibodies (mAbs) that bind and neutralize human pathogens have great therapeutic potential. Advances in automated screening and liquid handling have resulted in the ability to discover antigen-specific antibodies either directly from human blood or from various combinatorial libraries (phage, bacteria, or yeast). There remain, however, bottlenecks in the cloning, expression and evaluation of such lead antibodies identified in primary screens that hinder high-throughput screening. As such, "hit-to-lead identification" remains both expensive and time-consuming. By combining the advantages of overlap extension PCR (OE-PCR) and a genetically stable yet easily manipulatable microbial expression host Pichia pastoris, we have developed an automated pipeline for the rapid production and screening of full-length antigen-specific mAbs. Here, we demonstrate the speed, feasibility and cost-effectiveness of our approach by generating several broadly neutralizing antibodies against human immunodeficiency virus (HIV). PMID:26032261

  3. Automated IR determination of petroleum products in water based on sequential injection analysis.

    PubMed

    Falkova, Marina; Vakh, Christina; Shishov, Andrey; Zubakina, Ekaterina; Moskvin, Aleksey; Moskvin, Leonid; Bulatov, Andrey

    2016-02-01

    The simple and easy performed automated method for the IR determination of petroleum products (PP) in water using extraction-chromatographic cartridges has been developed. The method assumes two stages: on-site extraction of PP during a sampling by using extraction-chromatographic cartridges and subsequent determination of the extracted PP using sequential injection analysis (SIA) with IR detection. The appropriate experimental conditions for extraction of the dissolved in water PP and for automated SIA procedure were investigated. The calibration plot constructed using the developed procedure was linear in the range of 3-200 μg L(-1). The limit of detection (LOD), calculated from a blank test based on 3σ was 1 µg L(-1). The sample volume was 1L. The system throughput was found to be 12 h(-1). PMID:26653498

  4. Verification of Software Product Lines with Delta-Oriented Slicing

    NASA Astrophysics Data System (ADS)

    Bruns, Daniel; Klebanov, Vladimir; Schaefer, Ina

    Software product line (SPL) engineering is a well-known approach to develop industry-size adaptable software systems. SPL are often used in domains where high-quality software is desirable; the overwhelming product diversity, however, remains a challenge for assuring correctness. In this paper, we present delta-oriented slicing, an approach to reduce the deductive verification effort across an SPL where individual products are Java programs and their relations are described by deltas. On the specification side, we extend the delta language to deal with formal specifications. On the verification side, we combine proof slicing and similarity-guided proof reuse to ease the verification process.

  5. Targeted genetic modification of cell lines for recombinant protein production

    PubMed Central

    Piskareva, Olga; Muniyappa, Mohan

    2007-01-01

    Considerable increases in productivity have been achieved in biopharmaceutical production processes over the last two decades. Much of this has been a result of improvements in media formulation and process development. Though advances have been made in cell line development, there remains considerable opportunity for improvement in this area. The wealth of transcriptional and proteomic data being generated currently hold the promise of specific molecular interventions to improve the performance of production cell lines in the bioreactor. Achieving this—particularly for multi-gene modification—will require specific, targeted and controlled genetic manipulation of these cells. This review considers some of the current and potential future techniques that might be employed to realise this goal. PMID:19003191

  6. Evaluating space station applications of automation and robotics technologies from a human productivity point of view

    NASA Technical Reports Server (NTRS)

    Bard, J. F.

    1986-01-01

    The role that automation, robotics, and artificial intelligence will play in Space Station operations is now beginning to take shape. Although there is only limited data on the precise nature of the payoffs that these technologies are likely to afford there is a general consensus that, at a minimum, the following benefits will be realized: increased responsiveness to innovation, lower operating costs, and reduction of exposure to hazards. Nevertheless, the question arises as to how much automation can be justified with the technical and economic constraints of the program? The purpose of this paper is to present a methodology which can be used to evaluate and rank different approaches to automating the functions and tasks planned for the Space Station. Special attention is given to the impact of advanced automation on human productivity. The methodology employed is based on the Analytic Hierarchy Process. This permits the introduction of individual judgements to resolve the confict that normally arises when incomparable criteria underly the selection process. Because of the large number of factors involved in the model, the overall problem is decomposed into four subproblems individually focusing on human productivity, economics, design, and operations, respectively. The results from each are then combined to yield the final rankings. To demonstrate the methodology, an example is developed based on the selection of an on-orbit assembly system. Five alternatives for performing this task are identified, ranging from an astronaut working in space, to a dexterous manipulator with sensory feedback. Computational results are presented along with their implications. A final parametric analysis shows that the outcome is locally insensitive to all but complete reversals in preference.

  7. Automated poultry carcass inspection by hyperspectral-multispectral line-scan imaging system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Visible/near-infrared spectroscopy methods first developed by ARS scientists were demonstrated capable of over 90 percent accuracies on high-speed processing lines in differentiating wholesome chickens from unwholesome birds exhibiting systemic conditions; however, given the lack of spatial informat...

  8. Matrix Metalloproteinase-9 Production by Immortalized Human Chondrocyte Lines

    PubMed Central

    Malemud, Charles J.; Meszaros, Evan C.; Wylie, Meredith A.; Dahoud, Wissam; Skomorovska-Prokvolit, Yelenna; Mesiano, Sam

    2016-01-01

    We reported at the Keynote Forum of Immunology Summit-2015 that recombinant human (rh) TNF-α or rhIL-6 stimulated production of matrix metalloproteinase-9 (MMP-9) in the T/C28a2 and C-28/I2 human immortalized chondrocyte cell lines. Furthermore, we reported that tocilizumab (TCZ), a fully humanized monoclonal antibody which neutralizes IL-6-mediated signaling, inhibited the rhIL-6-mediated increase in the production of MMP-9. IL-6 is also a known activator of the JAK/STAT signaling pathway. In that regard, we evaluated the effect of rhIL-6 on total and phosphorylated Signal Transducer and Activator of Transcription by these chondrocyte lines which showed that whereas STAT3 was constitutively phosphorylated in T/C28a2 chondrocytes, rhIL-6 activated STAT3 in C-28/I2 chondrocytes. The finding that rhIL-6 increased the production of MMP-9 by human immortalized chondrocyte cell lines may have important implications with respect to the destruction of articular cartilage in rheumatoid arthritis and osteoarthritis. Thus, the markedly elevated level of IL-6 in rheumatoid arthritis and osteoarthritis sera and synovial fluid would be expected to generate significant MMP-9 to cause the degradation of articular cartilage extracellular matrix proteins. The finding that TCZ suppressed rhIL-6-mediated MMP-9 production suggests that TCZ, currently employed in the medical therapy of rheumatoid arthritis, could be considered as a drug for osteoarthritis.

  9. Automated fiber placement composite manufacturing: The mission at MSFC's Productivity Enhancement Complex

    NASA Technical Reports Server (NTRS)

    Vickers, John H.; Pelham, Larry I.

    1993-01-01

    Automated fiber placement is a manufacturing process used for producing complex composite structures. It is a notable leap to the state-of-the-art in technology for automated composite manufacturing. The fiber placement capability was established at the Marshall Space Flight Center's (MSFC) Productivity Enhancement Complex in 1992 in collaboration with Thiokol Corporation to provide materials and processes research and development, and to fabricate components for many of the Center's Programs. The Fiber Placement System (FPX) was developed as a distinct solution to problems inherent to other automated composite manufacturing systems. This equipment provides unique capabilities to build composite parts in complex 3-D shapes with concave and other asymmetrical configurations. Components with complex geometries and localized reinforcements usually require labor intensive efforts resulting in expensive, less reproducible components; the fiber placement system has the features necessary to overcome these conditions. The mechanical systems of the equipment have the motion characteristics of a filament winder and the fiber lay-up attributes of a tape laying machine, with the additional capabilities of differential tow payout speeds, compaction and cut-restart to selectively place the correct number of fibers where the design dictates. This capability will produce a repeatable process resulting in lower cost and improved quality and reliability.

  10. Data fusion of extremely high resolution aerial imagery and LiDAR data for automated railroad centre line reconstruction

    NASA Astrophysics Data System (ADS)

    Beger, Reinhard; Gedrange, Claudia; Hecht, Robert; Neubert, Marco

    2011-12-01

    The quality of remotely sensed data in regards of accuracy and resolution has considerably improved in recent years. Very small objects are detectable by means of imaging and laser scanning, yet there are only few studies to use such data for large scale mapping of railroad infrastructure.In this paper, an approach is presented that integrates extremely high resolution ortho-imagery and dense airborne laser scanning point clouds. These data sets are used to reconstruct railroad track centre lines. A feature level data fusion is carried out in order to combine the advantages of both data sets and to achieve a maximum of accuracy and completeness.The workflow consists of three successive processing steps. First, object-based image analysis is used to derive a railroad track mask from ortho-imagery. This spatial location information is then combined with the height information to classify the laser points. Lastly, the location of railroad track centre lines from these classified points were approximated using a feature extraction method based on an adapted random sample consensus algorithm. This workflow is tested on two railroad sections and was found to deliver very accurate results in a quickly and highly automated manner.

  11. Automated DNA extraction platforms offer solutions to challenges of assessing microbial biofouling in oil production facilities.

    PubMed

    Oldham, Athenia L; Drilling, Heather S; Stamps, Blake W; Stevenson, Bradley S; Duncan, Kathleen E

    2012-01-01

    The analysis of microbial assemblages in industrial, marine, and medical systems can inform decisions regarding quality control or mitigation. Modern molecular approaches to detect, characterize, and quantify microorganisms provide rapid and thorough measures unbiased by the need for cultivation. The requirement of timely extraction of high quality nucleic acids for molecular analysis is faced with specific challenges when used to study the influence of microorganisms on oil production. Production facilities are often ill equipped for nucleic acid extraction techniques, making the preservation and transportation of samples off-site a priority. As a potential solution, the possibility of extracting nucleic acids on-site using automated platforms was tested. The performance of two such platforms, the Fujifilm QuickGene-Mini80™ and Promega Maxwell®16 was compared to a widely used manual extraction kit, MOBIO PowerBiofilm™ DNA Isolation Kit, in terms of ease of operation, DNA quality, and microbial community composition. Three pipeline biofilm samples were chosen for these comparisons; two contained crude oil and corrosion products and the third transported seawater. Overall, the two more automated extraction platforms produced higher DNA yields than the manual approach. DNA quality was evaluated for amplification by quantitative PCR (qPCR) and end-point PCR to generate 454 pyrosequencing libraries for 16S rRNA microbial community analysis. Microbial community structure, as assessed by DGGE analysis and pyrosequencing, was comparable among the three extraction methods. Therefore, the use of automated extraction platforms should enhance the feasibility of rapidly evaluating microbial biofouling at remote locations or those with limited resources. PMID:23168231

  12. Automated DNA extraction platforms offer solutions to challenges of assessing microbial biofouling in oil production facilities

    PubMed Central

    2012-01-01

    The analysis of microbial assemblages in industrial, marine, and medical systems can inform decisions regarding quality control or mitigation. Modern molecular approaches to detect, characterize, and quantify microorganisms provide rapid and thorough measures unbiased by the need for cultivation. The requirement of timely extraction of high quality nucleic acids for molecular analysis is faced with specific challenges when used to study the influence of microorganisms on oil production. Production facilities are often ill equipped for nucleic acid extraction techniques, making the preservation and transportation of samples off-site a priority. As a potential solution, the possibility of extracting nucleic acids on-site using automated platforms was tested. The performance of two such platforms, the Fujifilm QuickGene-Mini80™ and Promega Maxwell®16 was compared to a widely used manual extraction kit, MOBIO PowerBiofilm™ DNA Isolation Kit, in terms of ease of operation, DNA quality, and microbial community composition. Three pipeline biofilm samples were chosen for these comparisons; two contained crude oil and corrosion products and the third transported seawater. Overall, the two more automated extraction platforms produced higher DNA yields than the manual approach. DNA quality was evaluated for amplification by quantitative PCR (qPCR) and end-point PCR to generate 454 pyrosequencing libraries for 16S rRNA microbial community analysis. Microbial community structure, as assessed by DGGE analysis and pyrosequencing, was comparable among the three extraction methods. Therefore, the use of automated extraction platforms should enhance the feasibility of rapidly evaluating microbial biofouling at remote locations or those with limited resources. PMID:23168231

  13. Automated apparatus for solvent separation of a coal liquefaction product stream

    DOEpatents

    Schweighardt, Frank K.

    1985-01-01

    An automated apparatus for the solvent separation of a coal liquefaction product stream that operates continuously and unattended and eliminates potential errors resulting from subjectivity and the aging of the sample during analysis. In use of the apparatus, metered amounts of one or more solvents are passed sequentially through a filter containing the sample under the direction of a microprocessor control means. The mixture in the filter is agitated by means of ultrasonic cavitation for a timed period and the filtrate is collected. The filtrate of each solvent extraction is collected individually and the residue on the filter element is collected to complete the extraction process.

  14. Gallium-68 DOTATATE Production with Automated PET Radiopharmaceutical Synthesis System: A Three Year Experience

    PubMed Central

    Aslani, Alireza; Snowdon, Graeme M; Bailey, Dale L; Schembri, Geoffrey P; Bailey, Elizabeth A; Roach, Paul J

    2014-01-01

    Objective(s): Gallium-68 (Ga-68) is an ideal research and hospital-based PET radioisotope. Currently, the main form of Ga-68 radiopharmaceutical that is being synthesised in-house is Ga-68 conjugated with DOTA based derivatives. The development of automated synthesis systems has increased the reliability, reproducibility and safety of radiopharmaceutical productions. Here we report on our three year, 500 syntheses experience with an automated system for Ga-68 DOTATATE. Methods: The automated synthesis system we use is divided into three parts of a) servomotor modules, b) single use sterile synthesis cassettes and, c) a computerised system that runs the modules. An audit trail is produced by the system as a requirement for GMP production. The required reagents and chemicals are made in-. The Germanium breakthrough is determined on a weekly basis. Production yields for each synthesis are calculated to monitor the performance and efficiency of the synthesis. The quality of the final product is assessed after each synthesis by ITLC-SG and HPLC methods. Results: A total of 500 Ga-68 DOTATATE syntheses (>800 patient doses) were performed between March 2011 and February 2014. The average generator yield was 81.3±0.2% for 2011, 76.7±0.4% for 2012 and 75.0±0.3% for 2013. Ga-68 DOTATATE yields for 2011, 2012, and 2013 were 81.8±0.4%, 82.2±0.4% and 87.9±0.4%, respectively. These exceed the manufacturer's expected value of approximately 70%. Germanium breakthrough averaged 8.6×10-6% of total activity which is well below the recommended level of 0.001%. The average ITLC-measured radiochemical purity was above 98.5% and the average HPLC-measured radiochemical purity was above 99.5%. Although there were some system failures during synthesis, there were only eight occasions where the patient scans needed to be rescheduled. Conclusion: In our experience the automated synthesis system performs reliably with a relatively low incident of failures. Our system had a consistent

  15. Automated generation of image products for Mars Exploration Rover Mission tactical operations

    NASA Technical Reports Server (NTRS)

    Alexander, Doug; Zamani, Payam; Deen, Robert; Andres, Paul; Mortensen, Helen

    2005-01-01

    This paper will discuss, from design to implementation, the methodologies applied to MIPL's automated pipeline processing as a 'system of systems' integrated with the MER GDS. Overviews of the interconnected product generating systems will also be provided with emphasis on interdependencies, including those for a) geometric rectificationn of camera lens distortions, b) generation of stereo disparity, c) derivation of 3-dimensional coordinates in XYZ space, d) generation of unified terrain meshes, e) camera-to-target ranging (distance) and f) multi-image mosaicking.

  16. SAGA: A project to automate the management of software production systems

    NASA Technical Reports Server (NTRS)

    Campbell, Roy H.; Beckman-Davies, C. S.; Benzinger, L.; Beshers, G.; Laliberte, D.; Render, H.; Sum, R.; Smith, W.; Terwilliger, R.

    1986-01-01

    Research into software development is required to reduce its production cost and to improve its quality. Modern software systems, such as the embedded software required for NASA's space station initiative, stretch current software engineering techniques. The requirements to build large, reliable, and maintainable software systems increases with time. Much theoretical and practical research is in progress to improve software engineering techniques. One such technique is to build a software system or environment which directly supports the software engineering process, i.e., the SAGA project, comprising the research necessary to design and build a software development which automates the software engineering process. Progress under SAGA is described.

  17. Automated 2D-3D registration of a radiograph and a cone beam CT using line-segment enhancement

    SciTech Connect

    Munbodh, Reshma; Jaffray, David A.; Moseley, Douglas J.; Chen Zhe; Knisely, Jonathan P.S.; Cathier, Pascal; Duncan, James S.

    2006-05-15

    The objective of this study was to develop a fully automated two-dimensional (2D)-three-dimensional (3D) registration framework to quantify setup deviations in prostate radiation therapy from cone beam CT (CBCT) data and a single AP radiograph. A kilovoltage CBCT image and kilovoltage AP radiograph of an anthropomorphic phantom of the pelvis were acquired at 14 accurately known positions. The shifts in the phantom position were subsequently estimated by registering digitally reconstructed radiographs (DRRs) from the 3D CBCT scan to the AP radiographs through the correlation of enhanced linear image features mainly representing bony ridges. Linear features were enhanced by filtering the images with ''sticks,'' short line segments which are varied in orientation to achieve the maximum projection value at every pixel in the image. The mean (and standard deviations) of the absolute errors in estimating translations along the three orthogonal axes in millimeters were 0.134 (0.096) AP(out-of-plane), 0.021 (0.023) ML and 0.020 (0.020) SI. The corresponding errors for rotations in degrees were 0.011 (0.009) AP, 0.029 (0.016) ML (out-of-plane), and 0.030 (0.028) SI (out-of-plane). Preliminary results with megavoltage patient data have also been reported. The results suggest that it may be possible to enhance anatomic features that are common to DRRs from a CBCT image and a single AP radiography of the pelvis for use in a completely automated and accurate 2D-3D registration framework for setup verification in prostate radiotherapy. This technique is theoretically applicable to other rigid bony structures such as the cranial vault or skull base and piecewise rigid structures such as the spine.

  18. Automating data analysis during the inspection of boiler tubes using line scanning thermography

    NASA Astrophysics Data System (ADS)

    Ley, Obdulia; Momeni, Sepand; Ostroff, Jason; Godinez, Valery

    2012-05-01

    Failures in boiler waterwalls can occur when a relatively small amount of corrosion and loss of metal have been experienced. This study presents our efforts towards the application of Line Scanning Thermography (LST) for the analysis of thinning in boiler waterwall tubing. LST utilizes a line heat source to thermally excite the surface to be inspected and an infrared detector to record the transient surface temperature increase observed due to the presence of voids, thinning or other defects. In waterwall boiler tubes the defects that can be detected using LST correspond to corrosion pitting, hydrogen damage and wall thinning produced by inadequate burner heating or problems with the water chemistry. In this paper we discuss how the LST technique is implemented to determine thickness from the surface temperature data, and we describe our efforts towards developing a semiautomatic analysis tool to speed up the time between scanning, reporting and implementing repairs. We compare the density of data produced by the common techniques used to assess wall thickness and the data produced by LST.

  19. 40 CFR 1054.310 - How must I select engines for production-line testing?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... production-line testing? 1054.310 Section 1054.310 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... EQUIPMENT Production-line Testing § 1054.310 How must I select engines for production-line testing? (a) Test... each test period, randomly select and test an engine from the end of the assembly line for each...

  20. Development of an automated high temperature valveless injection system for on-line gas chromatography

    NASA Astrophysics Data System (ADS)

    Kreisberg, N. M.; Worton, D. R.; Zhao, Y.; Isaacman, G.; Goldstein, A. H.; Hering, S. V.

    2014-07-01

    A reliable method of sample introduction is presented for on-line gas chromatography with a special application to in-situ field portable atmospheric sampling instruments. A traditional multi-port valve is replaced with a controlled pressure switching device that offers the advantage of long term reliability and stable sample transfer efficiency. An engineering design model is presented and tested that allows customizing the interface for other applications. Flow model accuracy is within measurement accuracy (1%) when parameters are tuned for an ambient detector and 15% accurate when applied to a vacuum based detector. Laboratory comparisons made between the two methods of sample introduction using a thermal desorption aerosol gas chromatograph (TAG) show approximately three times greater reproducibility maintained over the equivalent of a week of continuous sampling. Field performance results for two versions of the valveless interface used in the in-situ instrument demonstrate minimal trending and a zero failure rate during field deployments ranging up to four weeks of continuous sampling. Extension of the VLI to dual collection cells is presented with less than 3% cell-to-cell carry-over.

  1. Automated kidney detection for 3D ultrasound using scan line searching

    NASA Astrophysics Data System (ADS)

    Noll, Matthias; Nadolny, Anne; Wesarg, Stefan

    2016-04-01

    Ultrasound (U/S) is a fast and non-expensive imaging modality that is used for the examination of various anatomical structures, e.g. the kidneys. One important task for automatic organ tracking or computer-aided diagnosis is the identification of the organ region. During this process the exact information about the transducer location and orientation is usually unavailable. This renders the implementation of such automatic methods exceedingly challenging. In this work we like to introduce a new automatic method for the detection of the kidney in 3D U/S images. This novel technique analyses the U/S image data along virtual scan lines. Here, characteristic texture changes when entering and leaving the symmetric tissue regions of the renal cortex are searched for. A subsequent feature accumulation along a second scan direction produces a 2D heat map of renal cortex candidates, from which the kidney location is extracted in two steps. First, the strongest candidate as well as its counterpart are extracted by heat map intensity ranking and renal cortex size analysis. This process exploits the heat map gap caused by the renal pelvis region. Substituting the renal pelvis detection with this combined cortex tissue feature increases the detection robustness. In contrast to model based methods that generate characteristic pattern matches, our method is simpler and therefore faster. An evaluation performed on 61 3D U/S data sets showed, that in 55 cases showing none or minor shadowing the kidney location could be correctly identified.

  2. Development of an automated high temperature valveless injection system for on-line gas chromatography

    DOE PAGESBeta

    Kreisberg, N. M.; Worton, D. R.; Zhao, Y.; Isaacman, G.; Goldstein, A. H.; Hering, S. V.

    2014-07-23

    A reliable method of sample introduction is presented for on-line gas chromatography with a special application to in-situ field portable atmospheric sampling instruments. A traditional multi-port valve is replaced with a controlled pressure switching device that offers the advantage of long term reliability and stable sample transfer efficiency. An engineering design model is presented and tested that allows customizing the interface for other applications. Flow model accuracy is within measurement accuracy (1%) when parameters are tuned for an ambient detector and 15% accurate when applied to a vacuum based detector. Laboratory comparisons made between the two methods of sample introductionmore » using a thermal desorption aerosol gas chromatograph (TAG) show approximately three times greater reproducibility maintained over the equivalent of a week of continuous sampling. Field performance results for two versions of the valveless interface used in the in-situ instrument demonstrate minimal trending and a zero failure rate during field deployments ranging up to four weeks of continuous sampling. Extension of the VLI to dual collection cells is presented with less than 3% cell-to-cell carry-over.« less

  3. Automated Flight Dynamics Product Generation for the EOS AM-1 Spacecraft

    NASA Technical Reports Server (NTRS)

    Matusow, Carla

    1999-01-01

    As part of NASA's Earth Science Enterprise, the Earth Observing System (EOS) AM-1 spacecraft is designed to monitor long-term, global, environmental changes. Because of the complexity of the AM-1 spacecraft, the mission operations center requires more than 80 distinct flight dynamics products (reports). To create these products, the AM-1 Flight Dynamics Team (FDT) will use a combination of modified commercial software packages (e.g., Analytical Graphic's Satellite ToolKit) and NASA-developed software applications. While providing the most cost-effective solution to meeting the mission requirements, the integration of these software applications raises several operational concerns: (1) Routine product generation requires knowledge of multiple applications executing on variety of hardware platforms. (2) Generating products is a highly interactive process requiring a user to interact with each application multiple times to generate each product. (3) Routine product generation requires several hours to complete. (4) User interaction with each application introduces the potential for errors, since users are required to manually enter filenames and input parameters as well as run applications in the correct sequence. Generating products requires some level of flight dynamics expertise to determine the appropriate inputs and sequencing. To address these issues, the FDT developed an automation software tool called AutoProducts, which runs on a single hardware platform and provides all necessary coordination and communication among the various flight dynamics software applications. AutoProducts, autonomously retrieves necessary files, sequences and executes applications with correct input parameters, and deliver the final flight dynamics products to the appropriate customers. Although AutoProducts will normally generate pre-programmed sets of routine products, its graphical interface allows for easy configuration of customized and one-of-a-kind products. Additionally, AutoProducts

  4. Automated system for generation of soil moisture products for agricultural drought assessment

    NASA Astrophysics Data System (ADS)

    Raja Shekhar, S. S.; Chandrasekar, K.; Sesha Sai, M. V. R.; Diwakar, P. G.; Dadhwal, V. K.

    2014-11-01

    Drought is a frequently occurring disaster affecting lives of millions of people across the world every year. Several parameters, indices and models are being used globally to forecast / early warning of drought and monitoring drought for its prevalence, persistence and severity. Since drought is a complex phenomenon, large number of parameter/index need to be evaluated to sufficiently address the problem. It is a challenge to generate input parameters from different sources like space based data, ground data and collateral data in short intervals of time, where there may be limitation in terms of processing power, availability of domain expertise, specialized models & tools. In this study, effort has been made to automate the derivation of one of the important parameter in the drought studies viz Soil Moisture. Soil water balance bucket model is in vogue to arrive at soil moisture products, which is widely popular for its sensitivity to soil conditions and rainfall parameters. This model has been encoded into "Fish-Bone" architecture using COM technologies and Open Source libraries for best possible automation to fulfill the needs for a standard procedure of preparing input parameters and processing routines. The main aim of the system is to provide operational environment for generation of soil moisture products by facilitating users to concentrate on further enhancements and implementation of these parameters in related areas of research, without re-discovering the established models. Emphasis of the architecture is mainly based on available open source libraries for GIS and Raster IO operations for different file formats to ensure that the products can be widely distributed without the burden of any commercial dependencies. Further the system is automated to the extent of user free operations if required with inbuilt chain processing for every day generation of products at specified intervals. Operational software has inbuilt capabilities to automatically

  5. Human cell lines: A promising alternative for recombinant FIX production.

    PubMed

    de Sousa Bomfim, Aline; Cristina Corrêa de Freitas, Marcela; Picanço-Castro, Virgínia; de Abreu Soares Neto, Mário; Swiech, Kamilla; Tadeu Covas, Dimas; Maria de Sousa Russo, Elisa

    2016-05-01

    Factor IX (FIX) is a vitamin K-dependent protein, and it has become a valuable pharmaceutical in the Hemophilia B treatment. We evaluated the potential of recombinant human FIX (rhFIX) expression in 293T and SK-Hep-1 human cell lines. SK-Hep-1-FIX cells produced higher levels of biologically active protein. The growth profile of 293T-FIX cells was not influenced by lentiviral integration number into the cellular genome. SK-Hep-1-FIX cells showed a significantly lower growth rate than SK-Hep-1 cells. γ-carboxylation process is significant to FIX biological activity, thus we performed a expression analysis of genes involved in this process. The 293T gene expression suggests that this cell line could efficiently carboxylate FIX, however only 28% of the total secreted protein is active. SK-Hep-1 cells did not express high amounts of VKORC1 and carboxylase, but this cell line secreted large amounts of active protein. Enrichment of culture medium with Ca(+2) and Mg(+2) ions did not affect positively rhFIX expression in SK-Hep-1 cells. In 293T cells, the addition of 0.5 mM Ca(+2) and 1 mM Mg(+2) resulted in higher rhFIX concentration. SK-Hep-1 cell line proved to be very effective in rhFIX production, and it can be used as a novel biotechnological platform for the production of recombinant proteins. PMID:26802680

  6. High performance liquid chromatography for quantification of gatifloxacin in rat plasma following automated on-line solid phase extraction.

    PubMed

    Tasso, Leandro; Dalla Costa, Teresa

    2007-05-01

    An automated system using on-line solid phase extraction and HPLC with fluorimetric detection was developed and validated for quantification of gatifloxacin in rat plasma. The extraction was carried out using C(18) cartridges (BondElut), with a high extraction yield. After washing, gatifloxacin was eluted from the cartridge with mobile phase onto a C(18) HPLC column. The mobile phase consisted of a mixture of phosphoric acid (2.5mM), methanol, acetonitrile and triethylamine (64.8:15:20:0.2, v/v/v/v, apparent pH(app.) 2.8). All samples and standard solutions were chromatographed at 28 degrees C. The method developed was selective and linear for drug concentrations ranging between 20 and 600 ng/ml. Gatifloxacin recovery ranged from 95.6 to 99.7%, and the limit of quantification was 20 ng/ml. The intra and inter-assay accuracy were up to 94.3%. The precision determined not exceed 5.8% of the CV. High extraction yield up to 95% was obtained. Drug stability in plasma was shown in freezer at -20 degrees C up to 1 month, after three freeze-thaw cycles and for 24h in the autosampler after processing. The assay has been successfully applied to measure gatifloxacin plasma concentrations in pharmacokinetic study in rats. PMID:17403594

  7. A prototype for automation of land-cover products from Landsat Surface Reflectance Data Records

    NASA Astrophysics Data System (ADS)

    Rover, J.; Goldhaber, M. B.; Steinwand, D.; Nelson, K.; Coan, M.; Wylie, B. K.; Dahal, D.; Wika, S.; Quenzer, R.

    2014-12-01

    Landsat data records of surface reflectance provide a three-decade history of land surface processes. Due to the vast number of these archived records, development of innovative approaches for automated data mining and information retrieval were necessary. Recently, we created a prototype utilizing open source software libraries for automatically generating annual Anderson Level 1 land cover maps and information products from data acquired by the Landsat Mission for the years 1984 to 2013. The automated prototype was applied to two target areas in northwestern and east-central North Dakota, USA. The approach required the National Land Cover Database (NLCD) and two user-input target acquisition year-days. The Landsat archive was mined for scenes acquired within a 100-day window surrounding these target dates, and then cloud-free pixels where chosen closest to the specified target acquisition dates. The selected pixels were then composited before completing an unsupervised classification using the NLCD. Pixels unchanged in pairs of the NLCD were used for training decision tree models in an iterative process refined with model confidence measures. The decision tree models were applied to the Landsat composites to generate a yearly land cover map and related information products. Results for the target areas captured changes associated with the recent expansion of oil shale production and agriculture driven by economics and policy, such as the increase in biofuel production and reduction in Conservation Reserve Program. Changes in agriculture, grasslands, and surface water reflect the local hydrological conditions that occurred during the 29-year span. Future enhancements considered for this prototype include a web-based client, ancillary spatial datasets, trends and clustering algorithms, and the forecasting of future land cover.

  8. Product-line performance reporting: a key to cost management.

    PubMed

    Nackel, J G; Fenaroli, P J; Kis, G M

    1987-11-01

    For many hospitals, performance reporting is the most important and valuable function of a cost management system. Performance reporting provides hospitals--or any organization, for that matter--with an enhanced ability to reduce and control costs and monitor profitability. By periodically comparing strategic and operational plans with actual results, managers are provided with measurements of their performance for the aspects of the organization they control. The following is an excerpt from the book, Cost Management for Hospitals. This section deals with product-line performance reporting and the variances that measure the effects different factors have on performance. PMID:10284490

  9. Process development for automated solar cell and module production. Task 4. Automated array assembly. Quarterly report No. 1

    SciTech Connect

    Hagerty, J. J.

    1980-10-15

    Work has been divided into five phases. The first phase is to modify existing hardware and controlling computer software to: (1) improve cell-to-cell placement accuracy, (2) improve the solder joint while reducing the amount of solder and flux smear on the cell's surface, and (3) reduce the system cycle time to 10 seconds. The second phase involves expanding the existing system's capabilities to be able to reject broken cells and make post-solder electrical tests. Phase 3 involves developing new hardware to allow for the automated encapsulation of solar modules. This involves three discrete pieces of hardware: (1) a vacuum platen end effector for the robot which allows it to pick up the 1' x 4' array of 35 inter-connected cells. With this, it can also pick up the cover glass and completed module, (2) a lamination preparation station which cuts the various encapsulation components from roll storage and positions them for encapsulation, and (3) an automated encapsulation chamber which interfaces with the above two and applies the heat and vacuum to cure the encapsulants. Phase 4 involves the final assembly of the encapsulated array into a framed, edge-sealed module completed for installation. For this we are using MBA's Glass Reinforced Concrete (GRC) in panels such as those developed by MBA for JPL under contract No. 955281. The GRC panel plays the multiple role of edge frame, substrate and mounting structure. An automated method of applying the edge seal will also be developed. The final phase (5) is the fabrication of six 1' x 4' electrically active solar modules using the above developed equipment. Progress is reported. (WHK)

  10. Flow injection analysis-based methodology for automatic on-line monitoring and quality control for biodiesel production.

    PubMed

    Pinzi, S; Priego Capote, F; Ruiz Jiménez, J; Dorado, M P; Luque de Castro, M D

    2009-01-01

    An automated on-line approach based on determination of free and bound glycerol was here proposed to monitor biodiesel production. The method was based on liquid-liquid extraction of glycerol from the biodiesel to an aqueous ethanolic phase in which glycerol is oxidized to formaldehyde with meta periodate with subsequent reaction with acetylacetone. The reaction product was photometrically measured at 410 nm. Free and bound glycerol were differentiated by glycerides hydrolysis with potassium ethylate. The experimental set-up consisted of a flow-injection manifold for liquid-liquid extraction without phase separation and iterative change of the flow direction that enabled: (a) filling the flow manifold with a meta periodate-acetylacetone acceptor phase; (b) sampling of small amounts (microl) from the reactor; (c) determination of free glycerol by extraction from biodiesel to the aqueous phase with simultaneous oxidation-reaction with acetylacetone in the acceptor phase; (d) continuous monitoring of the aqueous phase by passage through a photometric detector; (e) filling the flow manifold with a potassium ethylate-meta periodate-acetylacetone new acceptor phase; (d) repetition of steps b-to-d to determine total glycerol after saponification of the bound glycerol by potassium ethylate; and (f) determination of bound glycerol by difference between the second and first analyses. The results showed that the proposed automated on-line method is a suitable option in routine analysis during biodiesel production. PMID:18614358

  11. Heparanase augments inflammatory chemokine production from colorectal carcinoma cell lines.

    PubMed

    Tsunekawa, Naoki; Higashi, Nobuaki; Kogane, Yusuke; Waki, Michihiko; Shida, Hiroaki; Nishimura, Yoshio; Adachi, Hayamitsu; Nakajima, Motowo; Irimura, Tatsuro

    2016-01-22

    To explore possible roles of heparanase in cancer-host crosstalk, we examined whether heparanase influences expression of inflammatory chemokines in colorectal cancer cells. Murine colorectal carcinoma cells incubated with heparanase upregulated MCP-1, KC, and RANTES genes and released MCP-1 and KC proteins. Heparanase-dependent production of IL-8 was detected in two human colorectal carcinoma cell lines. Addition of a heparanase inhibitor Heparastatin (SF4) did not influence MCP-1 production, while both latent and mature forms of heparanase augmented MCP-1 release, suggesting that heparanase catalytic activity was dispensable for MCP-1 production. In contrast, addition of heparin to the medium suppressed MCP-1 release in a dose-dependent manner. Similarly, targeted suppression of Ext1 by RNAi significantly suppressed cell surface expression of heparan sulfate and MCP-1 production in colon 26 cells. Taken together, it is concluded that colon 26 cells transduce the heparanase-mediated signal through heparan sulfate binding. We propose a novel function for heparanase independent of its endoglycosidase activity, namely as a stimulant for chemokine production. PMID:26713365

  12. An automated perfusion bioreactor for the streamlined production of engineered osteogenic grafts.

    PubMed

    Ding, Ming; Henriksen, Susan S; Wendt, David; Overgaard, Søren

    2016-04-01

    A computer-controlled perfusion bioreactor was developed for the streamlined production of engineered osteogenic grafts. This system automated the required bioprocesses, from the initial filling of the system through the phases of cell seeding and prolonged cell/tissue culture. Flow through chemo-optic micro-sensors allowed to non-invasively monitor the levels of oxygen and pH in the perfused culture medium throughout the culture period. To validate its performance, freshly isolated ovine bone marrow stromal cells were directly seeded on porous scaffold granules (hydroxyapatite/β-tricalcium-phosphate/poly-lactic acid), bypassing the phase of monolayer cell expansion in flasks. Either 10 or 20 days after culture, engineered cell-granule grafts were implanted in an ectopic mouse model to quantify new bone formation. After four weeks of implantation, histomorphometry showed more bone in bioreactor-generated grafts than cell-free granule controls, while bone formation did not show significant differences between 10 days and 20 days of incubation. The implanted granules without cells had no bone formation. This novel perfusion bioreactor has revealed the capability of activation larger viable bone graft material, even after shorter incubation time of graft material. This study has demonstrated the feasibility of engineering osteogenic grafts in an automated bioreactor system, laying the foundation for a safe, regulatory-compliant, and cost-effective manufacturing process. PMID:25952142

  13. Automated microextraction sample preparation coupled on-line to FT-ICR-MS: application to desalting and concentration of river and marine dissolved organic matter.

    PubMed

    Morales-Cid, Gabriel; Gebefugi, Istvan; Kanawati, Basem; Harir, Mourad; Hertkorn, Norbert; Rosselló-Mora, Ramón; Schmitt-Kopplin, Philippe

    2009-10-01

    Sample preparation procedures are in most cases sample- and time-consuming and commonly require the use of a large amount of solvents. Automation in this regard can optimize the minimal-needed injection volume and the solvent consumption will be efficiently reduced. A new fully automated sample desalting and pre-concentration technique employing microextraction by packed sorbents (MEPS) cartridges is implemented and coupled to an ion cyclotron resonance Fourier-transform mass spectrometer (ICR-FT/MS). The performance of non-target mass spectrometric analysis is compared for the automated versus off-line sample preparation for several samples of aqueous natural organic matter. This approach can be generalized for any metabolite profiling or metabolome analysis of biological materials but was optimized herein using a well characterized but highly complex organic mixture: a surface water and its well-characterized natural organic matter and a marine sample having a highly salt charge and enabling to validate the presented automatic system for salty samples. The analysis of Suwannee River water showed selective C18-MEPS enrichment of chemical signatures with average H/C and O/C elemental ratios and loss of both highly polar and highly aromatic structures from the original sample. Automated on-line application to marine samples showed desalting and different chemical signatures from surface to bottom water. Relative comparison of structural footprints with the C18-concentration/desalting procedure however enabled to demonstrate that the surface water film was more concentrated in surface-active components of natural (fatty acids) and anthropogenic origin (sulfur-containing surfactants). Overall, the relative standard deviation distribution in terms of peak intensity was improved by automating the proposed on-line method. PMID:19685041

  14. Automated Planning of Science Products Based on Nadir Overflights and Alerts for Onboard and Ground Processing

    NASA Technical Reports Server (NTRS)

    Chien, Steve A.; McLaren, David A.; Rabideau, Gregg R.; Mandl, Daniel; Hengemihle, Jerry

    2013-01-01

    A set of automated planning algorithms is the current operations baseline approach for the Intelligent Payload Module (IPM) of the proposed Hyper spectral Infrared Imager (HyspIRI) mission. For this operations concept, there are only local (e.g. non-depletable) operations constraints, such as real-time downlink and onboard memory, and the forward sweeping algorithm is optimal for determining which science products should be generated onboard and on ground based on geographical overflights, science priorities, alerts, requests, and onboard and ground processing constraints. This automated planning approach was developed for the HyspIRI IPM concept. The HyspIRI IPM is proposed to use an X-band Direct Broadcast (DB) capability that would enable data to be delivered to ground stations virtually as it is acquired. However, the HyspIRI VSWIR and TIR instruments will produce approximately 1 Gbps data, while the DB capability is 15 Mbps for a approx. =60X oversubscription. In order to address this mismatch, this innovation determines which data to downlink based on both the type of surface the spacecraft is overflying, and the onboard processing of data to detect events. For example, when the spacecraft is overflying Polar Regions, it might downlink a snow/ice product. Additionally, the onboard software will search for thermal signatures indicative of a volcanic event or wild fire and downlink summary information (extent, spectra) when detected, thereby reducing data volume. The planning system described above automatically generated the IPM mission plan based on requested products, the overflight regions, and available resources.

  15. Fully Automated One-Step Production of Functional 3D Tumor Spheroids for High-Content Screening.

    PubMed

    Monjaret, François; Fernandes, Mathieu; Duchemin-Pelletier, Eve; Argento, Amelie; Degot, Sébastien; Young, Joanne

    2016-04-01

    Adoption of spheroids within high-content screening (HCS) has lagged behind high-throughput screening (HTS) due to issues with running complex assays on large three-dimensional (3D) structures.To enable multiplexed imaging and analysis of spheroids, different cancer cell lines were grown in 3D on micropatterned 96-well plates with automated production of nine uniform spheroids per well. Spheroids achieve diameters of up to 600 µm, and reproducibility was experimentally validated (interwell and interplate CV(diameter) <5%). Biphoton imaging confirmed that micropatterned spheroids exhibit characteristic cell heterogeneity with distinct microregions. Furthermore, central necrosis appears at a consistent spheroid size, suggesting standardized growth.Using three reference compounds (fluorouracil, irinotecan, and staurosporine), we validated HT-29 micropatterned spheroids on an HCS platform, benchmarking against hanging-drop spheroids. Spheroid formation and imaging in a single plate accelerate assay workflow, and fixed positioning prevents structures from overlapping or sticking to the well wall, augmenting image processing reliability. Furthermore, multiple spheroids per well increase the statistical confidence sufficiently to discriminate compound mechanisms of action and generate EC50 values for endpoints of cell death, architectural change, and size within a single-pass read. Higher quality data and a more efficient HCS work chain should encourage integration of micropatterned spheroid models within fundamental research and drug discovery applications. PMID:26385905

  16. Decreasing costs of ground data processing system development using a software product line

    NASA Technical Reports Server (NTRS)

    Chaffin, Brian

    2005-01-01

    In this paper, I describe software product lines and why a Ground Data Processing System should use one. I also describe how to develop a software product line, using examples from an imaginary Ground Data Processing System.

  17. Horizon: The Portable, Scalable, and Reusable Framework for Developing Automated Data Management and Product Generation Systems

    NASA Astrophysics Data System (ADS)

    Huang, T.; Alarcon, C.; Quach, N. T.

    2014-12-01

    Capture, curate, and analysis are the typical activities performed at any given Earth Science data center. Modern data management systems must be adaptable to heterogeneous science data formats, scalable to meet the mission's quality of service requirements, and able to manage the life-cycle of any given science data product. Designing a scalable data management doesn't happen overnight. It takes countless hours of refining, refactoring, retesting, and re-architecting. The Horizon data management and workflow framework, developed at the Jet Propulsion Laboratory, is a portable, scalable, and reusable framework for developing high-performance data management and product generation workflow systems to automate data capturing, data curation, and data analysis activities. The NASA's Physical Oceanography Distributed Active Archive Center (PO.DAAC)'s Data Management and Archive System (DMAS) is its core data infrastructure that handles capturing and distribution of hundreds of thousands of satellite observations each day around the clock. DMAS is an application of the Horizon framework. The NASA Global Imagery Browse Services (GIBS) is NASA's Earth Observing System Data and Information System (EOSDIS)'s solution for making high-resolution global imageries available to the science communities. The Imagery Exchange (TIE), an application of the Horizon framework, is a core subsystem for GIBS responsible for data capturing and imagery generation automation to support the EOSDIS' 12 distributed active archive centers and 17 Science Investigator-led Processing Systems (SIPS). This presentation discusses our ongoing effort in refining, refactoring, retesting, and re-architecting the Horizon framework to enable data-intensive science and its applications.

  18. Automated High-Content Assay for Compounds Selectively Toxic to Trypanosoma cruzi in a Myoblastic Cell Line

    PubMed Central

    Alonso-Padilla, Julio; Cotillo, Ignacio; Presa, Jesús L.; Cantizani, Juan; Peña, Imanol; Bardera, Ana I.; Martín, Jose J.; Rodriguez, Ana

    2015-01-01

    Background Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, represents a very important public health problem in Latin America where it is endemic. Although mostly asymptomatic at its initial stage, after the disease becomes chronic, about a third of the infected patients progress to a potentially fatal outcome due to severe damage of heart and gut tissues. There is an urgent need for new drugs against Chagas disease since there are only two drugs available, benznidazole and nifurtimox, and both show toxic side effects and variable efficacy against the chronic stage of the disease. Methodology/Principal Findings Genetically engineered parasitic strains are used for high throughput screening (HTS) of large chemical collections in the search for new anti-parasitic compounds. These assays, although successful, are limited to reporter transgenic parasites and do not cover the wide T. cruzi genetic background. With the aim to contribute to the early drug discovery process against Chagas disease we have developed an automated image-based 384-well plate HTS assay for T. cruzi amastigote replication in a rat myoblast host cell line. An image analysis script was designed to inform on three outputs: total number of host cells, ratio of T. cruzi amastigotes per cell and percentage of infected cells, which respectively provides one host cell toxicity and two T. cruzi toxicity readouts. The assay was statistically robust (Z´ values >0.6) and was validated against a series of known anti-trypanosomatid drugs. Conclusions/Significance We have established a highly reproducible, high content HTS assay for screening of chemical compounds against T. cruzi infection of myoblasts that is amenable for use with any T. cruzi strain capable of in vitro infection. Our visual assay informs on both anti-parasitic and host cell toxicity readouts in a single experiment, allowing the direct identification of compounds selectively targeted to the parasite. PMID:25615687

  19. 16 CFR 303.22 - Products containing linings, interlinings, fillings, and paddings.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE TEXTILE FIBER PRODUCTS IDENTIFICATION... required information as to textile fiber products, the fiber content of any linings, interlinings,...

  20. Laser light-section sensor automating the production of textile-reinforced composites

    NASA Astrophysics Data System (ADS)

    Schmitt, R.; Niggemann, C.; Mersmann, C.

    2009-05-01

    Due to their advanced weight-specific mechanical properties, the application of fibre-reinforced plastics (FRP) has been established as a key technology in several engineering areas. Textile-based reinforcement structures (Preform) in particular achieve a high structural integrity due to the multi-dimensional build-up of dry-fibre layers combined with 3D-sewing and further textile processes. The final composite parts provide enhanced damage tolerances through excellent crash-energy absorbing characteristics. For these reasons, structural parts (e.g. frame) will be integrated in next generation airplanes. However, many manufacturing processes for FRP are still involving manual production steps without integrated quality control. The non-automated production implies considerable process dispersion and a high rework rate. Before the final inspection there is no reliable information about the production status. This work sets metrology as the key to automation and thus an economically feasible production, applying a laser light-section sensor system (LLSS) to measure process quality and feed back the results to close control loops of the production system. The developed method derives 3D-measurements from height profiles acquired by the LLSS. To assure the textile's quality a full surface scan is conducted, detecting defects or misalignment by comparing the measurement results with a CAD model of the lay-up. The method focuses on signal processing of the height profiles to ensure a sub-pixel accuracy using a novel algorithm based on a non-linear least-square fitting to a set of sigmoid functions. To compare the measured surface points to the CAD model, material characteristics are incorporated into the method. This ensures that only the fibre layer of the textile's surface is included and gaps between the fibres or overlaying seams are neglected. Finally, determining the uncertainty in measurement according to the GUM-standard proofed the sensor system's accuracy

  1. Product lines in a complex marketplace: matching organizational strategy to buyer behavior.

    PubMed

    Zelman, W N; McLaughlin, C P

    1990-01-01

    Product-line strategy should be developed in relation to markets. This article focuses on designing product-line strategy in relation to four purchaser types: (1) traditional purchasers, (2) motivated purchasers, (3) HMO-type purchasers, and (4) PPO-type purchasers. In many cases, product-line strategy may have to adopt various combinations of the above. PMID:2351546

  2. 40 CFR 1042.305 - Preparing and testing production-line engines.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of hours you operated your emission-data engine for certifying the engine family (see 40 CFR part... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Preparing and testing production-line... AND VESSELS Testing Production-line Engines § 1042.305 Preparing and testing production-line...

  3. Production-line assembly of 150+ VIRUS spectrographs

    NASA Astrophysics Data System (ADS)

    Marshall, J. L.; Vattiat, Brian; DePoy, D. L.; Hill, Gary J.; Collins, Amanda D.; Lee, Hanshin; Allen, Richard D.; Kelz, Andreas; Bauer, Svend M.; Popow, Emil

    2010-07-01

    The Visual Integral-Field Replicable Unit Spectrograph (VIRUS) instrument is being built to support observations for the Hobby-Eberly Telescope Dark Energy Experiment (HETDEX) project. The instrument consists of 150+ identical fiber-fed integral field optical spectrographs. This instrument provides a unique challenge in astronomical instrumentation: each of the 150+ instruments must be identical and each component must be interchangeable amongst every other spectrograph in order to ease assembly and maintenance of the instrument. In this paper we describe plans for the production-line assembly of the spectrographs. In particular, we discuss the assembly procedures and design choices that will ensure uniformity of the spectrographs and support the project schedule.

  4. Automation pilot

    NASA Technical Reports Server (NTRS)

    1983-01-01

    An important concept of the Action Information Management System (AIMS) approach is to evaluate office automation technology in the context of hands on use by technical program managers in the conduct of human acceptance difficulties which may accompany the transition to a significantly changing work environment. The improved productivity and communications which result from application of office automation technology are already well established for general office environments, but benefits unique to NASA are anticipated and these will be explored in detail.

  5. Automated Urinalysis

    NASA Technical Reports Server (NTRS)

    1994-01-01

    Information from NASA Tech Briefs assisted DiaSys Corporation in the development of the R/S 2000 which automates urinalysis, eliminating most manual procedures. An automatic aspirator is inserted into a standard specimen tube, the "Sample" button is pressed, and within three seconds a consistent amount of urine sediment is transferred to a microscope. The instrument speeds up, standardizes, automates and makes urine analysis safer. Additional products based on the same technology are anticipated.

  6. AUTOMATED ANALYSIS OF AQUEOUS SAMPLES CONTAINING PESTICIDES, ACIDIC/BASIC/NEUTRAL SEMIVOLATILES AND VOLATILE ORGANIC COMPOUNDS BY SOLID PHASE EXTRACTION COUPLED IN-LINE TO LARGE VOLUME INJECTION GC/MS

    EPA Science Inventory

    Data is presented on the development of a new automated system combining solid phase extraction (SPE) with GC/MS spectrometry for the single-run analysis of water samples containing a broad range of organic compounds. The system uses commercially available automated in-line 10-m...

  7. Evaluation of an automated hydrolysis and extraction method for quantification of total fat and lipid classess in cereal products.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The utility of an automated acid hydrolysis-extraction (AHE) system was evaluated for extraction of fat for the quantification of total, saturated, polyunsaturated, monounsaturated, and trans fat in cereal products. Oil extracted by the AHE system was assessed for total fat gravimetrically and by c...

  8. What Automated Vocal Analysis Reveals about the Vocal Production and Language Learning Environment of Young Children with Autism

    ERIC Educational Resources Information Center

    Warren, Steven F.; Gilkerson, Jill; Richards, Jeffrey A.; Oller, D. Kimbrough; Xu, Dongxin; Yapanel, Umit; Gray, Sharmistha

    2010-01-01

    The study compared the vocal production and language learning environments of 26 young children with autism spectrum disorder (ASD) to 78 typically developing children using measures derived from automated vocal analysis. A digital language processor and audio-processing algorithms measured the amount of adult words to children and the amount of…

  9. Improving GPR Surveys Productivity by Array Technology and Fully Automated Processing

    NASA Astrophysics Data System (ADS)

    Morello, Marco; Ercoli, Emanuele; Mazzucchelli, Paolo; Cottino, Edoardo

    2016-04-01

    The realization of network infrastructures with lower environmental impact and the tendency to use digging technologies less invasive in terms of time and space of road occupation and restoration play a key-role in the development of communication networks. However, pre-existing buried utilities must be detected and located in the subsurface, to exploit the high productivity of modern digging apparatus. According to SUE quality level B+ both position and depth of subsurface utilities must be accurately estimated, demanding for 3D GPR surveys. In fact, the advantages of 3D GPR acquisitions (obtained either by multiple 2D recordings or by an antenna array) versus 2D acquisitions are well-known. Nonetheless, the amount of acquired data for such 3D acquisitions does not usually allow to complete processing and interpretation directly in field and in real-time, thus limiting the overall efficiency of the GPR acquisition. As an example, the "low impact mini-trench "technique (addressed in ITU - International Telecommunication Union - L.83 recommendation) requires that non-destructive mapping of buried services enhances its productivity to match the improvements of new digging equipment. Nowadays multi-antenna and multi-pass GPR acquisitions demand for new processing techniques that can obtain high quality subsurface images, taking full advantage of 3D data: the development of a fully automated and real-time 3D GPR processing system plays a key-role in overall optical network deployment profitability. Furthermore, currently available computing power suggests the feasibility of processing schemes that incorporate better focusing algorithms. A novel processing scheme, whose goal is the automated processing and detection of buried targets that can be applied in real-time to 3D GPR array systems, has been developed and fruitfully tested with two different GPR arrays (16 antennas, 900 MHz central frequency, and 34 antennas, 600 MHz central frequency). The proposed processing

  10. Systems, methods and apparatus for developing and maintaining evolving systems with software product lines

    NASA Technical Reports Server (NTRS)

    Hinchey, Michael G. (Inventor); Rash, James L. (Inventor); Pena, Joaquin (Inventor)

    2011-01-01

    Systems, methods and apparatus are provided through which an evolutionary system is managed and viewed as a software product line. In some embodiments, the core architecture is a relatively unchanging part of the system, and each version of the system is viewed as a product from the product line. Each software product is generated from the core architecture with some agent-based additions. The result may be a multi-agent system software product line.

  11. Progress in DRS production line for uncooled focal plane arrays

    NASA Astrophysics Data System (ADS)

    Han, Chien J.; Howard, Christopher G.; Howard, Philip E.; Ionescu, Adrian C.; Li, Chuan C.; Monson, John C.; Naranjo, Robert L.; Scholten, Myron J.; Sweeney, R. Michael; Strong, Roger L.; Sullivan, William; Teherani, Towfik H.

    2004-08-01

    To improve its capacity to meet customer needs, DRS Infrared Technologies began technology transfer of the VOx uncooled FPA process from its Anaheim facility to its Dallas facility in the Fall of 2002. The new facility delivered its first U3000 arrays (320x240, 51μm pitch) three months after the VOx deposition system was installed, and produced over 300 units of U3000 per month just twelve months after beginning the transfer. Process enhancements and tool upgrades have enabled excellent control of the microbolometer process. Today, this line selectively fabricates arrays with NETD varying from 30mK to 80mK in 15mK bins with less than 30 ms time constant. The same arrays also have low defect density of less than 2% dead pixels and no more than one row and one column out. The arrays are packaged in imager or radiometer (F/1.4) packages. DRS also transferred small and large format arrays with 25μm pitch under the PEO-Soldier Sensor Producibility to the Dallas facility. Production of the 25μm pitch devices is currently more that 100 units per month and is ramping up to meet customer demand. This paper reports on production progress on the U3000s and the status of U3500 and U6000 25μm pitch array.

  12. Automated Data Production for a Novel Airborne Multiangle Spectropolarimetric Imager (airmspi)

    NASA Astrophysics Data System (ADS)

    Jovanovic, V. M.; Bull, M.; Diner, D. J.; Geier, S.; Rheingans, B.

    2012-07-01

    A novel polarimetric imaging technique making use of rapid retardance modulation has been developed by JPL as a part of NASA's Instrument Incubator Program. It has been built into the Airborne Multiangle SpectroPolarimetric Imager (AirMSPI) under NASA's Airborne Instrument Technology Transition Program, and is aimed primarily at remote sensing of the amounts and microphysical properties of aerosols and clouds. AirMSPI includes an 8-band (355, 380, 445, 470, 555, 660, 865, 935 nm) pushbroom camera that measures polarization in a subset of the bands (470, 660, and 865 nm). The camera is mounted on a gimbal and acquires imagery in a configurable set of along-track viewing angles ranging between +67° and -67° relative to nadir. As a result, near simultaneous multi-angle, multi-spectral, and polarimetric measurements of the targeted areas at a spatial resolution ranging from 7 m to 20 m (depending on the viewing angle) can be derived. An automated data production system is being built to support high data acquisition rate in concert with co-registration and orthorectified mapping requirements. To date, a number of successful engineering checkout flights were conducted in October 2010, August-September 2011, and January 2012. Data products resulting from these flights will be presented.

  13. Automated Data Production For A Novel Airborne Multiangle Spectropolarimetric Imager (AIRMSPI)

    NASA Technical Reports Server (NTRS)

    Jovanovic, V .M.; Bull, M.; Diner, D. J.; Geier, S.; Rheingans, B.

    2012-01-01

    A novel polarimetric imaging technique making use of rapid retardance modulation has been developed by JPL as a part of NASA's Instrument Incubator Program. It has been built into the Airborne Multiangle SpectroPolarimetric Imager (AirMSPI) under NASA's Airborne Instrument Technology Transition Program, and is aimed primarily at remote sensing of the amounts and microphysical properties of aerosols and clouds. AirMSPI includes an 8-band (355, 380, 445, 470, 555, 660, 865, 935 nm) pushbroom camera that measures polarization in a subset of the bands (470, 660, and 865 nm). The camera is mounted on a gimbal and acquires imagery in a configurable set of along-track viewing angles ranging between +67 deg and -67 deg relative to nadir. As a result, near simultaneous multi-angle, multi-spectral, and polarimetric measurements of the targeted areas at a spatial resolution ranging from 7 m to 20 m (depending on the viewing angle) can be derived. An automated data production system is being built to support high data acquisition rate in concert with co-registration and orthorectified mapping requirements. To date, a number of successful engineering checkout flights were conducted in October 2010, August-September 2011, and January 2012. Data products resulting from these flights will be presented.

  14. Managing the Evolution of an Enterprise Architecture using a MAS-Product-Line Approach

    NASA Technical Reports Server (NTRS)

    Pena, Joaquin; Hinchey, Michael G.; Resinas, manuel; Sterritt, Roy; Rash, James L.

    2006-01-01

    We view an evolutionary system ns being n software product line. The core architecture is the unchanging part of the system, and each version of the system may be viewed as a product from the product line. Each "product" may be described as the core architecture with sonre agent-based additions. The result is a multiagent system software product line. We describe an approach to such n Software Product Line-based approach using the MaCMAS Agent-Oriented nzethoclology. The approach scales to enterprise nrchitectures as a multiagent system is an approprinre means of representing a changing enterprise nrchitectclre nnd the inferaction between components in it.

  15. Neural networks are paying off on the production line

    SciTech Connect

    Irving, B.

    1997-10-01

    One very small company is working overtime on a technology called neural networks and is bringing this technology onto the production lines of some of the largest manufacturers in the world. The innovator here is Native American Technologies Co., Golden, Colo. Jerry Jones, the company`s chief scientist, explained the reason for this great interest in neural networks. A former member of the faculty at Colorado School of Mines, Jones said the large manufacturers want robust operating conditions. They want to be able to understand their processes well enough so that they don`t have to have fixtures on their shop floors. Fixtures are expensive to buy and to maintain and they have been known to die on the shop floor. Industry wants software capable of searching for those most robust operating conditions. N.A. Technologies is working closely with the Colorado School of Mines and with AMET, Inc., Rexford, Idaho, on projects in arc welding, friction stir welding, resistance welding, laser beam welding and electron beam welding. All of these projects are using exactly the same architecture and the same software, so the cost of development is spread out over a wide number of projects and a large number of potential applications. The end result is that the cost for any one given process is significantly less than it would be by any other approach.

  16. Automation of the longwall mining system

    NASA Technical Reports Server (NTRS)

    Zimmerman, W.; Aster, R. W.; Harris, J.; High, J.

    1982-01-01

    Cost effective, safe, and technologically sound applications of automation technology to underground coal mining were identified. The longwall analysis commenced with a general search for government and industry experience of mining automation technology. A brief industry survey was conducted to identify longwall operational, safety, and design problems. The prime automation candidates resulting from the industry experience and survey were: (1) the shearer operation, (2) shield and conveyor pan line advance, (3) a management information system to allow improved mine logistics support, and (4) component fault isolation and diagnostics to reduce untimely maintenance delays. A system network analysis indicated that a 40% improvement in productivity was feasible if system delays associated with all of the above four areas were removed. A technology assessment and conceptual system design of each of the four automation candidate areas showed that state of the art digital computer, servomechanism, and actuator technologies could be applied to automate the longwall system.

  17. A first-generation software product line for data acquisition systems in astronomy

    NASA Astrophysics Data System (ADS)

    López-Ruiz, J. C.; Heradio, Rubén; Cerrada Somolinos, José Antonio; Coz Fernandez, José Ramón; López Ramos, Pablo

    2008-07-01

    This article presents a case study on developing a software product line for data acquisition systems in astronomy based on the Exemplar Driven Development methodology and the Exemplar Flexibilization Language tool. The main strategies to build the software product line are based on the domain commonality and variability, the incremental scope and the use of existing artifacts. It consists on a lean methodology with little impact on the organization, suitable for small projects, which reduces product line start-up time. Software Product Lines focuses on creating a family of products instead of individual products. This approach has spectacular benefits on reducing the time to market, maintaining the know-how, reducing the development costs and increasing the quality of new products. The maintenance of the products is also enhanced since all the data acquisition systems share the same product line architecture.

  18. Characterization of available automated external defibrillators in the market based on the product manuals in 2014

    PubMed Central

    Ho, Chik Leung; Cheng, Ka Wai; Ma, Tze Hang; Wong, Yau Hang; Cheng, Ka Lok; Kam, Chak Wah

    2016-01-01

    BACKGROUND: To popularize the wide-spread use of automated external defibrillator (AED) to save life in sudden cardiac arrest, we compared the strength and weakness of different types of AEDs to enable a sound selection based on regional requirement. METHODS: This was a retrospective descriptive study. Different types of AEDs were compared according to the information of AEDs from manuals and brochures provided by the manufacturers. Fifteen types of AEDs were divided into 3 groups, basic, intermediate and advanced. RESULTS: Lifeline™ AUTO AED had the best performance in price, portability and user-friendly among AEDs of basic level. It required less time for shock charging. Samaritan PAD defibrillator was superior in price, portability, durability and characteristic among AEDs of intermediate level. It had the longest warranty and highest protection against water and dust. Lifeline™ PRO AED had the best performance in most of the criteria among AEDs of advanced level and offered CPR video and manual mode for laypersons and clinicians respectively. CONCLUSION: Lifeline™ AUTO AED, Samaritan PAD defibrillator, Lifeline™ PRO AED are superior in AEDs of basic, intermediate and advanced levels, respectively. A feasible AED may be chosen by users according to the regional requirement and the current information about the best available products. PMID:27313810

  19. Technology modernization assessment flexible automation

    SciTech Connect

    Bennett, D.W.; Boyd, D.R.; Hansen, N.H.; Hansen, M.A.; Yount, J.A.

    1990-12-01

    The objectives of this report are: to present technology assessment guidelines to be considered in conjunction with defense regulations before an automation project is developed to give examples showing how assessment guidelines may be applied to a current project to present several potential areas where automation might be applied successfully in the depot system. Depots perform primarily repair and remanufacturing operations, with limited small batch manufacturing runs. While certain activities (such as Management Information Systems and warehousing) are directly applicable to either environment, the majority of applications will require combining existing and emerging technologies in different ways, with the special needs of depot remanufacturing environment. Industry generally enjoys the ability to make revisions to its product lines seasonally, followed by batch runs of thousands or more. Depot batch runs are in the tens, at best the hundreds, of parts with a potential for large variation in product mix; reconfiguration may be required on a week-to-week basis. This need for a higher degree of flexibility suggests a higher level of operator interaction, and, in turn, control systems that go beyond the state of the art for less flexible automation and industry in general. This report investigates the benefits and barriers to automation and concludes that, while significant benefits do exist for automation, depots must be prepared to carefully investigate the technical feasibility of each opportunity and the life-cycle costs associated with implementation. Implementation is suggested in two ways: (1) develop an implementation plan for automation technologies based on results of small demonstration automation projects; (2) use phased implementation for both these and later stage automation projects to allow major technical and administrative risk issues to be addressed. 10 refs., 2 figs., 2 tabs. (JF)

  20. One Year of ICTP Diploma Courses On-Line Using the Automated EyA Recording System

    ERIC Educational Resources Information Center

    Canessa, Enrique; Fonda, Carlo; Zennaro, Marco

    2009-01-01

    The 12-month pre-Ph.D. ICTP Diploma Courses in the fields of Condensed Matter Physics, High Energy Physics, Mathematics, Earth System Physics and Basics Physics have been recorded using the automated, low cost recording system called EyA developed in-house. We discuss the technical details on how these recordings were implemented, together with…

  1. Pep2Path: automated mass spectrometry-guided genome mining of peptidic natural products.

    PubMed

    Medema, Marnix H; Paalvast, Yared; Nguyen, Don D; Melnik, Alexey; Dorrestein, Pieter C; Takano, Eriko; Breitling, Rainer

    2014-09-01

    Nonribosomally and ribosomally synthesized bioactive peptides constitute a source of molecules of great biomedical importance, including antibiotics such as penicillin, immunosuppressants such as cyclosporine, and cytostatics such as bleomycin. Recently, an innovative mass-spectrometry-based strategy, peptidogenomics, has been pioneered to effectively mine microbial strains for novel peptidic metabolites. Even though mass-spectrometric peptide detection can be performed quite fast, true high-throughput natural product discovery approaches have still been limited by the inability to rapidly match the identified tandem mass spectra to the gene clusters responsible for the biosynthesis of the corresponding compounds. With Pep2Path, we introduce a software package to fully automate the peptidogenomics approach through the rapid Bayesian probabilistic matching of mass spectra to their corresponding biosynthetic gene clusters. Detailed benchmarking of the method shows that the approach is powerful enough to correctly identify gene clusters even in data sets that consist of hundreds of genomes, which also makes it possible to match compounds from unsequenced organisms to closely related biosynthetic gene clusters in other genomes. Applying Pep2Path to a data set of compounds without known biosynthesis routes, we were able to identify candidate gene clusters for the biosynthesis of five important compounds. Notably, one of these clusters was detected in a genome from a different subphylum of Proteobacteria than that in which the molecule had first been identified. All in all, our approach paves the way towards high-throughput discovery of novel peptidic natural products. Pep2Path is freely available from http://pep2path.sourceforge.net/, implemented in Python, licensed under the GNU General Public License v3 and supported on MS Windows, Linux and Mac OS X. PMID:25188327

  2. 76 FR 62343 - Certain Lined Paper Products From India: Notice of Preliminary Results of Antidumping Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-07

    ... FR 66349 (October 28, 2010. (Initiation Notice).\\2\\ \\1\\ Abhinav Paper Products Pvt. Ltd.; American... Critical Circumstances Determination: Certain Lined Paper Products from India, 71 FR 45034, 45035 (August 8... Countervailing Duty Orders: Certain Lined Paper Products from India and Indonesia, 71 FR 56949 (September...

  3. Automated system for the on-line monitoring of powder blending processes using near-infrared spectroscopy. Part I. System development and control.

    PubMed

    Hailey, P A; Doherty, P; Tapsell, P; Oliver, T; Aldridge, P K

    1996-03-01

    An automated system for the on-line monitoring of powder blending processes is described. The system employs near-infrared (NIR) spectroscopy using fibre-optics and a graphical user interface (GUI) developed in the LabVIEW environment. The complete supervisory control and data analysis (SCADA) software controls blender and spectrophotometer operation and performs statistical spectral data analysis in real time. A data analysis routine using standard deviation is described to demonstrate an approach to the real-time determination of blend homogeneity. PMID:8738184

  4. Integrated configurable equipment selection and line balancing for mass production with serial-parallel machining systems

    NASA Astrophysics Data System (ADS)

    Battaïa, Olga; Dolgui, Alexandre; Guschinsky, Nikolai; Levin, Genrikh

    2014-10-01

    Solving equipment selection and line balancing problems together allows better line configurations to be reached and avoids local optimal solutions. This article considers jointly these two decision problems for mass production lines with serial-parallel workplaces. This study was motivated by the design of production lines based on machines with rotary or mobile tables. Nevertheless, the results are more general and can be applied to assembly and production lines with similar structures. The designers' objectives and the constraints are studied in order to suggest a relevant mathematical model and an efficient optimization approach to solve it. A real case study is used to validate the model and the developed approach.

  5. Gamma ray line production from cosmic ray spallation reactions

    NASA Technical Reports Server (NTRS)

    Silberberg, R.; Tsao, C. H.; Letaw, J. R.

    1985-01-01

    The gamma ray line intensities due to cosmic ray spallation reactions in clouds, the galactic disk and accreting binary pulsars are calculated. With the most favorable plausible assumptions, only a few lines may be detectable to the level of 0.0000001 per sq. cm per sec. The intensities are compared with those generated in nuclear excitation reactions.

  6. Automated on-line renewable solid-phase extraction-liquid chromatography exploiting multisyringe flow injection-bead injection lab-on-valve analysis.

    PubMed

    Quintana, José Benito; Miró, Manuel; Estela, José Manuel; Cerdà, Víctor

    2006-04-15

    In this paper, the third generation of flow injection analysis, also named the lab-on-valve (LOV) approach, is proposed for the first time as a front end to high-performance liquid chromatography (HPLC) for on-line solid-phase extraction (SPE) sample processing by exploiting the bead injection (BI) concept. The proposed microanalytical system based on discontinuous programmable flow features automated packing (and withdrawal after single use) of a small amount of sorbent (<5 mg) into the microconduits of the flow network and quantitative elution of sorbed species into a narrow band (150 microL of 95% MeOH). The hyphenation of multisyringe flow injection analysis (MSFIA) with BI-LOV prior to HPLC analysis is utilized for on-line postextraction treatment to ensure chemical compatibility between the eluate medium and the initial HPLC gradient conditions. This circumvents the band-broadening effect commonly observed in conventional on-line SPE-based sample processors due to the low eluting strength of the mobile phase. The potential of the novel MSFI-BI-LOV hyphenation for on-line handling of complex environmental and biological samples prior to reversed-phase chromatographic separations was assessed for the expeditious determination of five acidic pharmaceutical residues (viz., ketoprofen, naproxen, bezafibrate, diclofenac, and ibuprofen) and one metabolite (viz., salicylic acid) in surface water, urban wastewater, and urine. To this end, the copolymeric divinylbenzene-co-n-vinylpyrrolidone beads (Oasis HLB) were utilized as renewable sorptive entities in the micromachined unit. The automated analytical method features relative recovery percentages of >88%, limits of detection within the range 0.02-0.67 ng mL(-1), and coefficients of variation <11% for the column renewable mode and gives rise to a drastic reduction in operation costs ( approximately 25-fold) as compared to on-line column switching systems. PMID:16615800

  7. SAGA: A project to automate the management of software production systems

    NASA Technical Reports Server (NTRS)

    Campbell, Roy H.; Laliberte, D.; Render, H.; Sum, R.; Smith, W.; Terwilliger, R.

    1987-01-01

    The Software Automation, Generation and Administration (SAGA) project is investigating the design and construction of practical software engineering environments for developing and maintaining aerospace systems and applications software. The research includes the practical organization of the software lifecycle, configuration management, software requirements specifications, executable specifications, design methodologies, programming, verification, validation and testing, version control, maintenance, the reuse of software, software libraries, documentation, and automated management.

  8. Alicyclobacillus Contamination in the Production Line of Kiwi Products in China

    PubMed Central

    Zhang, Jiangbo; Yue, Tianli; Yuan, Yahong

    2013-01-01

    Alicyclobacillus are spoilage microbes of many juice products, but contamination of kiwi products by Alicyclobacillus is seldom reported. This study aims to investigate the whole production line of kiwi products in China to assess the potential risk of their contamination. A total of 401 samples from 18 commercial products, 1 processing plant and 16 raw material orchards were tested, and 76 samples were positive, from which 76 strains of microbes were isolated and identified as 4 species of Alicyclobacillus, including Alicyclobacillus acidoterrestris, Alicyclobacillus contaminans, Alicyclobacillus herbarius and Alicyclobacillus cycloheptanicus, and another 9 strains as 3 species of Bacillus by sequencing of their 16S rDNA. Through phylogenetic tree construction and RAPD-PCR amplification, it was found that there exist genotypic diversities to some extent among these isolates. Four test strains (each from one species of the 4 Alicyclobacillus species isolated in this study) could spoil pH adjusted kiwi fruit juice and some commercial kiwi fruit products with producing guaiacol (11–34 ppb). PMID:23844069

  9. An automated system to mount cryo-cooled protein crystals on a synchrotron beam line, using compact sample cassettes and a small-scale robot

    PubMed Central

    Cohen, Aina E.; Ellis, Paul J.; Miller, Mitchell D.; Deacon, Ashley M.; Phizackerley, R. Paul

    2014-01-01

    An automated system for mounting and dismounting pre-frozen crystals has been implemented at the Stanford Synchrotron Radiation Laboratory (SSRL). It is based on a small industrial robot and compact cylindrical cassettes, each holding up to 96 crystals mounted on Hampton Research sample pins. For easy shipping and storage, the cassette fits inside several popular dry-shippers and long-term storage Dewars. A dispensing Dewar holds up to three cassettes in liquid nitrogen adjacent to the beam line goniometer. The robot uses a permanent magnet tool to extract samples from, and insert samples into a cassette, and a cryo-tong tool to transfer them to and from the beam line goniometer. The system is simple, with few moving parts, reliable in operation and convenient to use. PMID:24899734

  10. Automation in photogrammetry: Recent developments and applications (1972-1976)

    USGS Publications Warehouse

    Thompson, M.M.; Mikhail, E.M.

    1976-01-01

    An overview of recent developments in the automation of photogrammetry in various countries is presented. Conclusions regarding automated photogrammetry reached at the 1972 Congress in Ottawa are reviewed first as a background for examining the developments of 1972-1976. Applications are described for each country reporting significant developments. Among fifteen conclusions listed are statements concerning: the widespread practice of equipping existing stereoplotters with simple digitizers; the growing tendency to use minicomputers on-line with stereoplotters; the optimization of production of digital terrain models by progressive sampling in stereomodels; the potential of digitization of a photogrammetric model by density correlation on epipolar lines; the capabilities and economic aspects of advanced systems which permit simultaneous production of orthophotos, contours, and digital terrain models; the economy of off-line orthophoto systems; applications of digital image processing; automation by optical techniques; applications of sensors other than photographic imagery, and the role of photogrammetric phases in a completely automated cartographic system. ?? 1976.

  11. Automated power management and control

    NASA Technical Reports Server (NTRS)

    Dolce, James L.

    1991-01-01

    A comprehensive automation design is being developed for Space Station Freedom's electric power system. A joint effort between NASA's Office of Aeronautics and Exploration Technology and NASA's Office of Space Station Freedom, it strives to increase station productivity by applying expert systems and conventional algorithms to automate power system operation. The initial station operation will use ground-based dispatches to perform the necessary command and control tasks. These tasks constitute planning and decision-making activities that strive to eliminate unplanned outages. We perceive an opportunity to help these dispatchers make fast and consistent on-line decisions by automating three key tasks: failure detection and diagnosis, resource scheduling, and security analysis. Expert systems will be used for the diagnostics and for the security analysis; conventional algorithms will be used for the resource scheduling.

  12. Quantitative determination of opioids in whole blood using fully automated dried blood spot desorption coupled to on-line SPE-LC-MS/MS.

    PubMed

    Verplaetse, Ruth; Henion, Jack

    2016-01-01

    Opioids are well known, widely used painkillers. Increased stability of opioids in the dried blood spot (DBS) matrix compared to blood/plasma has been described. Other benefits provided by DBS techniques include point-of-care collection, less invasive micro sampling, more economical shipment, and convenient storage. Current methodology for analysis of micro whole blood samples for opioids is limited to the classical DBS workflow, including tedious manual punching of the DBS cards followed by extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalysis. The goal of this study was to develop and validate a fully automated on-line sample preparation procedure for the analysis of DBS micro samples relevant to the detection of opioids in finger prick blood. To this end, automated flow-through elution of DBS cards was followed by on-line solid-phase extraction (SPE) and analysis by LC-MS/MS. Selective, sensitive, accurate, and reproducible quantitation of five representative opioids in human blood at sub-therapeutic, therapeutic, and toxic levels was achieved. The range of reliable response (R(2)  ≥0.997) was 1 to 500 ng/mL whole blood for morphine, codeine, oxycodone, hydrocodone; and 0.1 to 50 ng/mL for fentanyl. Inter-day, intra-day, and matrix inter-lot accuracy and precision was less than 15% (even at lower limits of quantitation (LLOQ) level). The method was successfully used to measure hydrocodone and its major metabolite norhydrocodone in incurred human samples. Our data support the enormous potential of DBS sampling and automated analysis for monitoring opioids as well as other pharmaceuticals in both anti-doping and pain management regimens. PMID:26607771

  13. Automated extraction method for the center line of spinal canal and its application to the spinal curvature quantification in torso X-ray CT images

    NASA Astrophysics Data System (ADS)

    Hayashi, Tatsuro; Zhou, Xiangrong; Chen, Huayue; Hara, Takeshi; Miyamoto, Kei; Kobayashi, Tatsunori; Yokoyama, Ryujiro; Kanematsu, Masayuki; Hoshi, Hiroaki; Fujita, Hiroshi

    2010-03-01

    X-ray CT images have been widely used in clinical routine in recent years. CT images scanned by a modern CT scanner can show the details of various organs and tissues. This means various organs and tissues can be simultaneously interpreted on CT images. However, CT image interpretation requires a lot of time and energy. Therefore, support for interpreting CT images based on image-processing techniques is expected. The interpretation of the spinal curvature is important for clinicians because spinal curvature is associated with various spinal disorders. We propose a quantification scheme of the spinal curvature based on the center line of spinal canal on CT images. The proposed scheme consists of four steps: (1) Automated extraction of the skeletal region based on CT number thresholding. (2) Automated extraction of the center line of spinal canal. (3) Generation of the median plane image of spine, which is reformatted based on the spinal canal. (4) Quantification of the spinal curvature. The proposed scheme was applied to 10 cases, and compared with the Cobb angle that is commonly used by clinicians. We found that a high-correlation (for the 95% confidence interval, lumbar lordosis: 0.81-0.99) between values obtained by the proposed (vector) method and Cobb angle. Also, the proposed method can provide the reproducible result (inter- and intra-observer variability: within 2°). These experimental results suggested a possibility that the proposed method was efficient for quantifying the spinal curvature on CT images.

  14. Automation of the longwall mining system

    SciTech Connect

    Zimmerman, W.; Aster, R.; Harris, J.; High, J.

    1982-11-01

    The longwall automation study presented is the first phase of a study to evaluate mining automation opportunities. The objective was to identify cost-effective, safe, and technologically sound applications of automation technology to understand coal mining. The prime automation candidates resulting from the industry experience and survey were: (1) the shearer operation, (2) shield and conveyor pan-line advance, (3) a management information system to allow improved mine logistics support, and (4) component fault isolation and diagnostics to reduce untimely maintenance delays. A system network analysis indicated that a 40% improvement in productivity was feasible if system delays associated with all of the above four areas were removed. A technology assessment and conceptual system design of each of the four automation candidate areas showed that state-of-the-art digital computer, servomechanism, and actuator technologies could be applied to automate the longwall system. The final cost benefit analysis of all of the automation areas indicated a total net national benefit (profit) of roughly $200 million to the longwall mining industry if all automation candidates were installed. This cost benefit represented an approximate order of magnitude payback on the research and development (R and D) investment. In conclusion, it is recommended that the shearer operation be automated first because it provides a large number of other sensor inputs required for face alignment (i.e., shields and conveyor). Automation of the shield and conveyor pan-line advance is suggested as the next step since both the shearer and face alignment operations contributed the greatest time delays to the overall system downtime.

  15. Automated production of 124I and 64Cu using IBA Terimo and Pinctada metal electroplating and processing modules

    NASA Astrophysics Data System (ADS)

    Poniger, S. S.; Tochon-Danguy, H. J.; Panopoulos, H. P.; O'Keefe, G. J.; Peake, D.; Rasool, R.; Scott, A. M.

    2012-12-01

    There is worldwide growing interest for the production of long-lived positron emitters for molecular imaging and the development of novel immuno-PET techniques for drugs discovery. The desire to produce solid target isotopes in Australia has significantly increased over the years and several research projects for labelling of peptides, proteins and biomolecules, including labelling of recombinant antibodies has been limited due to the availability of suitable isotopes. This has led to the recent installation and commissioning of a new lab dedicated to fully automated solid target isotope production, including 124I, 64Cu, 89Zr and 86Y.

  16. Large-scale cell production of stem cells for clinical application using the automated cell processing machine

    PubMed Central

    2013-01-01

    Background Cell-based regeneration therapies have great potential for application in new areas in clinical medicine, although some obstacles still remain to be overcome for a wide range of clinical applications. One major impediment is the difficulty in large-scale production of cells of interest with reproducibility. Current protocols of cell therapy require a time-consuming and laborious manual process. To solve this problem, we focused on the robotics of an automated and high-throughput cell culture system. Automated robotic cultivation of stem or progenitor cells in clinical trials has not been reported till date. The system AutoCulture® used in this study can automatically replace the culture medium, centrifuge cells, split cells, and take photographs for morphological assessment. We examined the feasibility of this system in a clinical setting. Results We observed similar characteristics by both the culture methods in terms of the growth rate, gene expression profile, cell surface profile by fluorescence-activated cell sorting, surface glycan profile, and genomic DNA stability. These results indicate that AutoCulture® is a feasible method for the cultivation of human cells for regenerative medicine. Conclusions An automated cell-processing machine will play important roles in cell therapy and have widespread use from application in multicenter trials to provision of off-the-shelf cell products. PMID:24228851

  17. Investigation Of In-Line Monitoring Options At H Canyon/HB Line For Plutonium Oxide Production

    SciTech Connect

    Sexton, L.

    2015-10-14

    H Canyon and HB Line have a production goal of 1 MT per year of plutonium oxide feedstock for the MOX facility by FY17 (AFS-2 mission). In order to meet this goal, steps will need to be taken to improve processing efficiency. One concept for achieving this goal is to implement in-line process monitoring at key measurement points within the facilities. In-line monitoring during operations has the potential to increase throughput and efficiency while reducing costs associated with laboratory sample analysis. In the work reported here, we mapped the plutonium oxide process, identified key measurement points, investigated alternate technologies that could be used for in-line analysis, and initiated a throughput benefit analysis.

  18. 40 CFR 1045.301 - When must I test my production-line engines?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... require you to test production-line engines under this subpart, or under 40 CFR part 1068, subpart E, even... described in 40 CFR part 1068. Individual engines in families that pass these production-line testing requirements must also conform to all applicable regulations of this part and 40 CFR part 1068. (d) You may...

  19. A Role-Playing Game for a Software Engineering Lab: Developing a Product Line

    ERIC Educational Resources Information Center

    Zuppiroli, Sara; Ciancarini, Paolo; Gabbrielli, Maurizio

    2012-01-01

    Software product line development refers to software engineering practices and techniques for creating families of similar software systems from a basic set of reusable components, called shared assets. Teaching how to deal with software product lines in a university lab course is a challenging task, because there are several practical issues that…

  20. 40 CFR 1045.301 - When must I test my production-line engines?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... require you to test production-line engines under this subpart, or under 40 CFR part 1068, subpart E, even... described in 40 CFR part 1068. Individual engines in families that pass these production-line testing requirements must also conform to all applicable regulations of this part and 40 CFR part 1068. (d) You may...

  1. Intelligent Production Monitoring and Control based on Three Main Modules for Automated Manufacturing Cells in the Automotive Industry

    NASA Astrophysics Data System (ADS)

    Berger, Ulrich; Kretzschmann, Ralf; Algebra, A. Vargas Veronica

    2008-06-01

    The automotive industry is distinguished by regionalization and customization of products. As consequence, the diversity of products will increase while the lot sizes will decrease. Thus, more product types will be handled along the process chain and common production paradigms will fail. Although Rapid Manufacturing (RM) methodology will be used for producing small individual lot sizes, new solution for joining and assembling these components are needed. On the other hand, the non-availability of existing operational knowledge and the absence of dynamic and explicit knowledge retrieval minimize the achievement of on-demand capabilities. Thus, in this paper, an approach for an Intelligent Production System will be introduced. The concept is based on three interlinked main modules: a Technology Data Catalogue (TDC) based on an ontology system, an Automated Scheduling Processor (ASP) based on graph theory and a central Programmable Automation Controller (PAC) for real-time sensor/actor communication. The concept is being implemented in a laboratory set-up with several assembly and joining processes and will be experimentally validated in some research and development projects.

  2. Intelligent Production Monitoring and Control based on Three Main Modules for Automated Manufacturing Cells in the Automotive Industry

    SciTech Connect

    Berger, Ulrich; Kretzschmann, Ralf; Algebra, A. Vargas Veronica

    2008-06-12

    The automotive industry is distinguished by regionalization and customization of products. As consequence, the diversity of products will increase while the lot sizes will decrease. Thus, more product types will be handled along the process chain and common production paradigms will fail. Although Rapid Manufacturing (RM) methodology will be used for producing small individual lot sizes, new solution for joining and assembling these components are needed. On the other hand, the non-availability of existing operational knowledge and the absence of dynamic and explicit knowledge retrieval minimize the achievement of on-demand capabilities. Thus, in this paper, an approach for an Intelligent Production System will be introduced. The concept is based on three interlinked main modules: a Technology Data Catalogue (TDC) based on an ontology system, an Automated Scheduling Processor (ASP) based on graph theory and a central Programmable Automation Controller (PAC) for real-time sensor/actor communication. The concept is being implemented in a laboratory set-up with several assembly and joining processes and will be experimentally validated in some research and development projects.

  3. HYPERSPECTRAL/MULTISPECTRAL LINE-SCAN IMAGING SYSTEM FOR AUTOMATED POULTRY CARCASS INSPECTION APPLICATIONS FOR FOOD SAFETY

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral/multispectral line-scan imaging system was developed for differentiation of wholesome and systemically diseased chickens. In-plant testing was conducted for chickens on a commercial evisceration line moving at a speed of 70 birds per minute. Hyperspectral image data was acquired fo...

  4. Automated synthesis of transmission lines loaded with complementary split ring resonators (CSRRs) and open complementary split ring resonators (OCSRRs) through aggressive space mapping (ASM)

    NASA Astrophysics Data System (ADS)

    Selga, Jordi; Rodríguez, Ana; Orellana, Marco; Boria, Vicente; Martín, Ferran

    2014-09-01

    This paper is focused on the application of space mapping optimization to the automated synthesis of transmission lines loaded with complementary split ring resonators (CSRRs) and open complementary split ring resonators (OCSRRs). These structures are of interest for the implementation of resonant-type metamaterial transmission lines and for the design of planar microwave circuits based on such complementary resonators. The paper presents a method to generate the layouts of CSRR- and OCSRR-loaded microstrip lines from the elements of their equivalent circuit models. Using the so-called aggressive space mapping, a specific implementation that uses quasi-Newton type iteration, we have developed synthesis algorithms that are able to provide the topology of these CSRR- and OCSRR-loaded lines in few steps. The most relevant aspect, however, is that this synthesis process is completely automatic, i.e., it does not require any action from the designers, other than initiating the algorithm. Moreover, this technique can be translated to other electrically small planar elements described by lumped element equivalent circuit models.

  5. Validation and calibration of a TDLAS oxygen sensor for in-line measurement on flow-packed products

    NASA Astrophysics Data System (ADS)

    Cocola, L.; Fedel, M.; Allermann, H.; Landa, S.; Tondello, G.; Bardenstein, A.; Poletto, L.

    2016-05-01

    A device based on Tunable Diode Laser Absorption Spectroscopy has been developed for non-invasive evaluation of gaseous oxygen concentration inside packed food containers. This work has been done in the context of the SAFETYPACK European project in order to enable full, automated product testing on a production line. The chosen samples at the end of the manufacturing process are modified atmosphere bags of processed mozzarella, in which the target oxygen concentration is required to be below 5%. The spectrometer allows in-line measurement of moving samples which are passing on a conveyor belt, with an optical layout optimized for bags made of a flexible scattering material, and works by sensing the gas phase in the headspace at the top of the package. A field applicable method for the calibration of this device has been identified and validated against traditional, industry standard, invasive measurement techniques. This allows some degrees of freedom for the end-user regarding packaging dimensions and shape. After deployment and setup of the instrument at the end-user manufacturing site, performance has been evaluated on a different range of samples in order to validate the choice of electro optical and geometrical parameters regarding sample handling and measurement timing at the actual measurement conditions.

  6. 9 CFR 113.52 - Requirements for cell lines used for production of biologics.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... production of biologics. 113.52 Section 113.52 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION... STANDARD REQUIREMENTS Ingredient Requirements § 113.52 Requirements for cell lines used for production of biologics. When prescribed in an applicable Standard Requirement or in a filed Outline of Production...

  7. 9 CFR 113.52 - Requirements for cell lines used for production of biologics.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Requirements for cell lines used for production of biologics. 113.52 Section 113.52 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD REQUIREMENTS...

  8. The length of the glaciers in the world - a straightforward method for the automated calculation of glacier center lines

    NASA Astrophysics Data System (ADS)

    Machguth, H.; Huss, M.

    2014-05-01

    Glacier length is an important measure of glacier geometry but global glacier inventories are mostly lacking length data. Only recently semi-automated approaches to measure glacier length have been developed and applied regionally. Here we present a first global assessment of glacier length using a fully automated method based on glacier surface slope, distance to the glacier margins and a set of trade-off functions. The method is developed for East Greenland, evaluated for the same area as well as for Alaska, and eventually applied to all ∼200 000 glaciers around the globe. The evaluation highlights accurately calculated glacier length where DEM quality is good (East Greenland) and limited precision on low quality DEMs (parts of Alaska). Measured length of very small glaciers is subject to a certain level of ambiguity. The global calculation shows that only about 1.5% of all glaciers are longer than 10 km with Bering Glacier (Alaska/Canada) being the longest glacier in the world at a length of 196 km. Based on model output we derive global and regional area-length scaling laws. Differences among regional scaling parameters appear to be related to characteristics of topography and glacier mass balance. The present study adds glacier length as a central parameter to global glacier inventories. Global and regional scaling laws might proof beneficial in conceptual glacier models.

  9. A model for simulating the grinding and classification cyclic system of waste PCBs recycling production line.

    PubMed

    Yang, Deming; Xu, Zhenming

    2011-09-15

    Crushing and separating technology is widely used in waste printed circuit boards (PCBs) recycling process. A set of automatic line without negative impact to environment for recycling waste PCBs was applied in industry scale. Crushed waste PCBs particles grinding and classification cyclic system is the most important part of the automatic production line, and it decides the efficiency of the whole production line. In this paper, a model for computing the process of the system was established, and matrix analysis method was adopted. The result showed that good agreement can be achieved between the simulation model and the actual production line, and the system is anti-jamming. This model possibly provides a basis for the automatic process control of waste PCBs production line. With this model, many engineering problems can be reduced, such as metals and nonmetals insufficient dissociation, particles over-pulverizing, incomplete comminuting, material plugging and equipment fever. PMID:21764511

  10. Automation in biological crystallization

    PubMed Central

    Shaw Stewart, Patrick; Mueller-Dieckmann, Jochen

    2014-01-01

    Crystallization remains the bottleneck in the crystallographic process leading from a gene to a three-dimensional model of the encoded protein or RNA. Automation of the individual steps of a crystallization experiment, from the preparation of crystallization cocktails for initial or optimization screens to the imaging of the experiments, has been the response to address this issue. Today, large high-throughput crystallization facilities, many of them open to the general user community, are capable of setting up thousands of crystallization trials per day. It is thus possible to test multiple constructs of each target for their ability to form crystals on a production-line basis. This has improved success rates and made crystallization much more convenient. High-throughput crystallization, however, cannot relieve users of the task of producing samples of high quality. Moreover, the time gained from eliminating manual preparations must now be invested in the careful evaluation of the increased number of experiments. The latter requires a sophisticated data and laboratory information-management system. A review of the current state of automation at the individual steps of crystallization with specific attention to the automation of optimization is given. PMID:24915074

  11. Sensors and Automated Analyzers for Radionuclides

    SciTech Connect

    Grate, Jay W.; Egorov, Oleg B.

    2003-03-27

    The production of nuclear weapons materials has generated large quantities of nuclear waste and significant environmental contamination. We have developed new, rapid, automated methods for determination of radionuclides using sequential injection methodologies to automate extraction chromatographic separations, with on-line flow-through scintillation counting for real time detection. This work has progressed in two main areas: radionuclide sensors for water monitoring and automated radiochemical analyzers for monitoring nuclear waste processing operations. Radionuclide sensors have been developed that collect and concentrate radionuclides in preconcentrating minicolumns with dual functionality: chemical selectivity for radionuclide capture and scintillation for signal output. These sensors can detect pertechnetate to below regulatory levels and have been engineered into a prototype for field testing. A fully automated process monitor has been developed for total technetium in nuclear waste streams. This instrument performs sample acidification, speciation adjustment, separation and detection in fifteen minutes or less.

  12. An automated high-content screening image analysis pipeline for the identification of selective autophagic inducers in human cancer cell lines.

    PubMed

    Kriston-Vizi, Janos; Lim, Ching Aeng; Condron, Peter; Chua, Kelvin; Wasser, Martin; Flotow, Horst

    2010-08-01

    Automated image processing is a critical and often rate-limiting step in high-content screening (HCS) workflows. The authors describe an open-source imaging-statistical framework with emphasis on segmentation to identify novel selective pharmacological inducers of autophagy. They screened a human alveolar cancer cell line and evaluated images by both local adaptive and global segmentation. At an individual cell level, region-growing segmentation was compared with histogram-derived segmentation. The histogram approach allowed segmentation of a sporadic-pattern foreground and hence the attainment of pixel-level precision. Single-cell phenotypic features were measured and reduced after assessing assay quality control. Hit compounds selected by machine learning corresponded well to the subjective threshold-based hits determined by expert analysis. Histogram-derived segmentation displayed robustness against image noise, a factor adversely affecting region growing segmentation. PMID:20547532

  13. ELIXYS - a fully automated, three-reactor high-pressure radiosynthesizer for development and routine production of diverse PET tracers

    PubMed Central

    2013-01-01

    Background Automated radiosynthesizers are vital for routine production of positron-emission tomography tracers to minimize radiation exposure to operators and to ensure reproducible synthesis yields. The recent trend in the synthesizer industry towards the use of disposable kits aims to simplify setup and operation for the user, but often introduces several limitations related to temperature and chemical compatibility, thus requiring reoptimization of protocols developed on non-cassette-based systems. Radiochemists would benefit from a single hybrid system that provides tremendous flexibility for development and optimization of reaction conditions while also providing a pathway to simple, cassette-based production of diverse tracers. Methods We have designed, built, and tested an automated three-reactor radiosynthesizer (ELIXYS) to provide a flexible radiosynthesis platform suitable for both tracer development and routine production. The synthesizer is capable of performing high-pressure and high-temperature reactions by eliminating permanent tubing and valve connections to the reaction vessel. Each of the three movable reactors can seal against different locations on disposable cassettes to carry out different functions such as sealed reactions, evaporations, and reagent addition. A reagent and gas handling robot moves sealed reagent vials from storage locations in the cassette to addition positions and also dynamically provides vacuum and inert gas to ports on the cassette. The software integrates these automated features into chemistry unit operations (e.g., React, Evaporate, Add) to intuitively create synthesis protocols. 2-Deoxy-2-[18F]fluoro-5-methyl-β-l-arabinofuranosyluracil (l-[18F]FMAU) and 2-deoxy-2-[18F]fluoro-β-d-arabinofuranosylcytosine (d-[18F]FAC) were synthesized to validate the system. Results l-[18F]FMAU and d-[18F]FAC were successfully synthesized in 165 and 170 min, respectively, with decay-corrected radiochemical yields of 46% ± 1% (n = 6

  14. Investigation of Freeze-Linings in Aluminum Production Cells

    NASA Astrophysics Data System (ADS)

    Fallah-Mehrjardi, Ata; Hayes, Peter C.; Jak, Evgueni

    2014-08-01

    The molten cryolite bath creates chemically a very aggressive environment in the Hall-Héroult cell, and thus, the formation of a protective solid layer (freeze-lining) on the cell wall is essential for the operation of the present cell designs. To provide further information on the formation of the freeze-lining deposit in this system, laboratory-based studies were undertaken using an air-cooled probe technique The effects of process conditions, i.e., time, bath agitation, and superheat on the microstructures, morphologies of the phases, and the phase assemblages adjacent to the deposit/bath interface were investigated. A detailed microstructural analysis of the steady-state deposits shows that a dense sealing primary-phase layer of cryolite solid solution was formed at the interface of the bath deposit for the process conditions examined. The formation of sealing primary-phase layer at the bath/deposit interface explicitly indicates that the deposit/liquid bath interface temperature is equal to that of the liquidus of the bulk bath. The experimentally investigated liquidus temperature and subliquidus equilibria differ significantly from those previously reported.

  15. Automated production of [¹⁸F]VAT suitable for clinical PET study of vesicular acetylcholine transporter.

    PubMed

    Yue, Xuyi; Bognar, Christopher; Zhang, Xiang; Gaehle, Gregory G; Moerlein, Stephen M; Perlmutter, Joel S; Tu, Zhude

    2016-01-01

    Automated production of a promising radiopharmaceutical (-)-(1-(8-(2-[(18)F]fluoroethoxy)-3-hydroxy-1,2,3,4-tetrahydronaphthalen-2-yl)-piperidin-4-yl)(4-fluorophenyl)methanone ([(18)F]VAT) for the vesicular acetylcholine transporter(VAChT) was achieved using a two-step procedure in a current Good Manufacturing Practices fashion. The production of [(18)F]VAT was accomplished in approximately 140 min, with radiochemical yield of ~15.0% (decay corrected), specific activity>111 GBq/µmol, radiochemical purity>99% and mass of VAT ~3.4 μg/batch (n>10). The radiopharmaceutical product meets all quality control criteria for human use, and is suitable for clinical PET studies of VAChT. PMID:26408913

  16. Radiolytic hydrogen production from process vessels in HB line - production rates compared to evolution rates and discussion of LASL reviews

    SciTech Connect

    Bibler, N.E.

    1992-11-12

    Hydrogen production from radiolysis of aqueous solutions can create a safety hazard since hydrogen is flammable. At times this production can be significant, especially in HB line where nitric acid solutions containing high concentrations of Pu-238, an intense alpha emitter, are processed. The hydrogen production rates from these solutions are necessary for safety analyses of these process systems. The methods and conclusions of hydrogen production rate tests are provided in this report.

  17. New Claus and FCC catalyst production capacity on line

    SciTech Connect

    Not Available

    1983-07-01

    Two new, major catalyst manufacturing plants have recently gone into commercial operation in the U.S. In Louisiana, Aluminum Co. of America (Alcoa) last month put an activated alumina plant in production at Vidalia, La., on the Mississippi River near Natchez, Miss. Activated alumina after processing serves as, among other things, the catalyst in Claus sulfur recovery plants. In Georgia, Katalistiks International Inc. put its fluid catalytic cracking catalyst plant on stream in June, with an expected output of 60,000/80,000 tons/year of FFC catalyst. Alcoa says its Vidalia plant will have the capacity to make 20 million lb/year of either Claus catalyst or adsorbants, catalyst substrates, or combinations of such products. Management says it hopes to develop the catalyst plant's processing flexibility to make products for special and new applications. For the moment, the Vidalia plant will not make zeolites for fluid catalytic catalyst manufacturing though it has that capability. However, production of combinations of mixtures of alumina and zeolites is planned.

  18. 9 CFR 113.52 - Requirements for cell lines used for production of biologics.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... production of biologics. 113.52 Section 113.52 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION... Plant Health Inspection Service (APHIS) upon request for performing the tests prescribed in this section... line, such as, but not limited to, microscopic appearance, growth rate, acid production, or...

  19. A semi-automated large-scale process for the production of recombinant tagged proteins in the Baculovirus expression system.

    PubMed

    Schlaeppi, Jean-Marc; Henke, Mario; Mahnke, Marion; Hartmann, Steffen; Schmitz, Rita; Pouliquen, Yann; Kerins, Brendan; Weber, Eric; Kolbinger, Frank; Kocher, Hans P

    2006-12-01

    The efficient preparation of recombinant proteins at the lab-scale level is essential for drug discovery, in particular for structural biology, protein interaction studies and drug screening. The Baculovirus insect-cell expression system is one of the most widely applied and highly successful systems for production of recombinant functional proteins. However, the use of eukaryotic cells as host organisms and the multi-step protocol required for the generation of sufficient virus and protein has limited its adaptation to industrialized high-throughput operation. We have developed an integrated large-scale process for continuous and partially automated protein production in the Baculovirus system. The instrumental platform includes parallel insect-cell fermentation in 10L BioWave reactors, cell harvesting and lysis by tangential flow filtration (TFF) using two custom-made filtration units and automated purification by multi-dimensional chromatography. The use of disposable materials (bags, filters and tubing), automated cleaning cycles and column regeneration, prevent any cross-contamination between runs. The preparation of the clear cell lysate by sequential TFF takes less than 2 h and represents considerable time saving compared to standard cell harvesting and lysis by sonication and ultra-centrifugation. The process has been validated with 41 His-tagged proteins with molecular weights ranging from 20 to 160 kDa. These proteins represented several families, and included 23 members of the deubiquitinating enzyme (DUB) family. Each down-stream unit can process four proteins in less than 24 h with final yields between 1 and 100 mg, and purities between 50 and 95%. PMID:16904904

  20. Information logistics: A production-line approach to information services

    NASA Technical Reports Server (NTRS)

    Adams, Dennis; Lee, Chee-Seng

    1991-01-01

    Logistics can be defined as the process of strategically managing the acquisition, movement, and storage of materials, parts, and finished inventory (and the related information flow) through the organization and its marketing channels in a cost effective manner. It is concerned with delivering the right product to the right customer in the right place at the right time. The logistics function is composed of inventory management, facilities management, communications unitization, transportation, materials management, and production scheduling. The relationship between logistics and information systems is clear. Systems such as Electronic Data Interchange (EDI), Point of Sale (POS) systems, and Just in Time (JIT) inventory management systems are important elements in the management of product development and delivery. With improved access to market demand figures, logisticians can decrease inventory sizes and better service customer demand. However, without accurate, timely information, little, if any, of this would be feasible in today's global markets. Information systems specialists can learn from logisticians. In a manner similar to logistics management, information logistics is concerned with the delivery of the right data, to the ring customer, at the right time. As such, information systems are integral components of the information logistics system charged with providing customers with accurate, timely, cost-effective, and useful information. Information logistics is a management style and is composed of elements similar to those associated with the traditional logistics activity: inventory management (data resource management), facilities management (distributed, centralized and decentralized information systems), communications (participative design and joint application development methodologies), unitization (input/output system design, i.e., packaging or formatting of the information), transportations (voice, data, image, and video communication systems

  1. Automated flow-through amperometric immunosensor for highly sensitive and on-line detection of okadaic acid in mussel sample.

    PubMed

    Dominguez, Rocio B; Hayat, Akhtar; Sassolas, Audrey; Alonso, Gustavo A; Munoz, Roberto; Marty, Jean-Louis

    2012-09-15

    An electrochemical immunosensor for okadaic acid (OA) detection has been developed, and used in an indirect competitive immunoassay format under automated flow conditions. The biosensor was fabricated by injecting OA modified magnetic beads onto screen printed carbon electrode (SPCE) in the flow system. The OA present in the sample competed with the immobilized OA to bind with anti-okadaic acid monoclonal antibody (anti-OA-MAb). The secondary alkaline phosphatase labeled antibody was used to perform electrochemical detection. The current response obtained from the labeled alkaline phosphatase to 1-naphthyl phosphate decreased proportionally to the concentration of free OA in the sample. The calculated limit of detection (LOD) was 0.15 μg/L with a linear range of 0.19-25 μg/L. The good recoveries percentages validated the immunosensor application for real mussel samples. The developed system automatically controlled the incubation, washing and current measurement steps, showing its potential use for OA determination in field analysis. PMID:22967546

  2. 75 FR 7563 - Certain Lined Paper Products from India: Notice of Final Results of Antidumping Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-22

    ...: Certain Lined Paper Products from India and Indonesia, 71 FR 56949 (September 28, 2006) (Lined Paper... Preliminary Results of Antidumping Duty Administrative Review, 74 FR 51558 (October 7, 2009) (Preliminary.... See Antidumping and Countervailing Duty Proceedings: Assessment of Antidumping Duties, 68 FR...

  3. 77 FR 53174 - Certain Lined Paper Products From Indonesia: Revocation of Antidumping and Countervailing Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-31

    ..., 76 FR 45778 (August 1, 2011) and Certain Lined Paper School Supplies From China, India, and Indonesia... Republic of China, 76 FR 76123 (December 6, 2011) and Certain Lined Paper Products From Indonesia: Final... Paper School Supplies From China, India, and Indonesia, 77 FR 51570 (August 24, 2012). See also...

  4. Early attempts at production of prawn cell lines.

    PubMed

    Owens, L; Smith, J

    1999-01-01

    This report describes some unsuccessful attempts to produce continuous cell lines from penaeid prawn tissues in the late 1980s. This information is presented so that others might save time by not repeating the unsuccessful measures that were attempted. The osmolarity of Penaeus monodon haemolymph was measured at 687 mOsmol/kg (N = 10). Of the media tested, the best medium for cell growth and maintenance was shown to be double strength L-15, supplemented with 10% foetal bovine serum, and 10% prawn muscle extract at 28 degrees C ( approximately 675.5 mOsmol/kg). Prawn muscle extract was made by homogenizing 30 g of prawn muscle in 50/50 ratio of distilled water/autoclaved seawater, clarified stepwise by centrifugation at 2k, 14k, 14k xg for 30 minutes each. The resultant supernatant was heat-inactivated on occasions with no improvement in growth. Preconditioned medium, cholesterol, galactose and trehalose supplements and the use of Cell-Tak did not improve growth conditions, and haemolymph extracts were detrimental to the cells. In addition it was shown that Nunc 25 cm(2) plastic culture flasks were better than Linbro and both were better than glass as substrates. The fate of 101 individual primary cell cultures, established from penaeid prawns, was as follows. Fifteen of the cultures succumbed to bacterial contamination, five became contaminated with fungi, four with thauastrochytrids, four succumbed to presumptive viral autocultures and two to ciliate contamination. Cell cultures derived from heart tissue could be maintained for a mean of 12.7 days (sd 9.7d), those derived from the epidermis 15.6 days (sd 9.0d), ovarian tissue 10 days (sd 2d), lymphoid organ 6.8 days (sd 0.4d), nerve cord and hepatopancreas 2 days. The most persistent cell cultures -- those derived from the heart explants -- contained dividing cells at 40 days, and epidermis cells were still dividing at 30 days. The longest lasting, non- proliferating, but viable, cell cultures were those of

  5. Opitmizing a Beef Production System Using Specialized Sire and Dam Lines

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Crossbreeding is an effective method for improving efficiency of production in commercial cow-calf operations. It exploits available heterosis (hybrid vigor) and complementarity between different breeds or populations (lines). Before adopting a crossbreeding system, commercial cattle producers shou...

  6. Architecture-Based Unit Testing of the Flight Software Product Line

    NASA Technical Reports Server (NTRS)

    Ganesan, Dharmalingam; Lindvall, Mikael; McComas, David; Bartholomew, Maureen; Slegel, Steve; Medina, Barbara

    2010-01-01

    This paper presents an analysis of the unit testing approach developed and used by the Core Flight Software (CFS) product line team at the NASA GSFC. The goal of the analysis is to understand, review, and reconunend strategies for improving the existing unit testing infrastructure as well as to capture lessons learned and best practices that can be used by other product line teams for their unit testing. The CFS unit testing framework is designed and implemented as a set of variation points, and thus testing support is built into the product line architecture. The analysis found that the CFS unit testing approach has many practical and good solutions that are worth considering when deciding how to design the testing architecture for a product line, which are documented in this paper along with some suggested innprovennents.

  7. 40 CFR 1054.345 - What production-line testing records must I send to EPA?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Clean Air Act. Our production-line testing conformed completely with the requirements of 40 CFR part... type of engine. (8) Provide the CumSum analysis required in § 1054.315 and the sample-size...

  8. 40 CFR 1051.345 - What production-line testing records must I send to EPA?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Air Act. Our production-line testing conformed completely with the requirements of 40 CFR part 1051...) Provide the CumSum analysis required in § 1051.315 and the sample-size calculation required in §...

  9. 40 CFR 1054.345 - What production-line testing records must I send to EPA?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Clean Air Act. Our production-line testing conformed completely with the requirements of 40 CFR part... type of engine. (8) Provide the CumSum analysis required in § 1054.315 and the sample-size...

  10. 40 CFR 1051.345 - What production-line testing records must I send to EPA?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Air Act. Our production-line testing conformed completely with the requirements of 40 CFR part 1051...) Provide the CumSum analysis required in § 1051.315 and the sample-size calculation required in §...

  11. 40 CFR 1054.345 - What production-line testing records must I send to EPA?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Clean Air Act. Our production-line testing conformed completely with the requirements of 40 CFR part... type of engine. (8) Provide the CumSum analysis required in § 1054.315 and the sample-size...

  12. 40 CFR 1051.345 - What production-line testing records must I send to EPA?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Air Act. Our production-line testing conformed completely with the requirements of 40 CFR part 1051...) Provide the CumSum analysis required in § 1051.315 and the sample-size calculation required in §...

  13. 77 FR 61742 - Certain Lined Paper Products From India: Preliminary Results of Countervailing Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-11

    ...-Free Import of Capital Goods and Raw Materials 5. Market Development Assistance (MDA) 6. Market Access... Duty Orders: Certain Lined Paper Products from India and Indonesia, 71 FR 56949 (September 28,...

  14. 78 FR 62584 - Certain Lined Paper Products From India: Preliminary Results of Countervailing Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ... Goods and Raw Materials for Export Oriented Units (EOUs) VI. Conclusion BILLING CODE 3510-DS-P ... Duty Orders: Certain Lined Paper Products from India and Indonesia, 71 FR 56949 (September 28,...

  15. Comparison of antibody molecules produced from two cell lines with contrasting productivities and aggregate contents.

    PubMed

    Ishii, Yoichi; Imamoto, Yasufumi; Yamamoto, Rie; Tsukahara, Masayoshi; Wakamatsu, Kaori

    2015-01-01

    Cell culture processes that produce therapeutic antibodies with high productivity (titer) and low aggregate content reduce the risk of adverse effects and expense to patients. To elucidate the mechanism of aggregate formation, we compared trastuzumab samples produced from two contrasting cell lines: cell line A, which exhibits high titer and low aggregate content, and cell line B, which exhibits low titer and high aggregate content. Cell line B produced significantly fewer (approximately 1/3) antibodies compared with cell line A and contained higher (approximately 3-fold) percentages of aggregates. The aggregates of antibodies found in the protein A-purified samples of cell line B were associated mostly with noncovalent interactions. Cell line B exhibited a low content of monomers/dimers of light chains in the medium and within cells. Because light chains are essential for the correct folding of heavy chains and secretion of mature antibodies, the characteristics of cell line B may be attributed to low levels of light chain production. In addition, protein A-purified antibodies from cell line B (but not those from cell line A) contained fragments that are expected to expose the hydrophobic CH3 domain, which may serve as nuclei for aggregation. PMID:25501618

  16. Lines

    ERIC Educational Resources Information Center

    Mires, Peter B.

    2006-01-01

    National Geography Standards for the middle school years generally stress the teaching of latitude and longitude. There are many creative ways to explain the great grid that encircles our planet, but the author has found that students in his college-level geography courses especially enjoy human-interest stories associated with lines of latitude…

  17. Increasing Student Interest and Comprehension of Production Planning and Control and Operations Performance Measurement Concepts Using a Production Line Game

    ERIC Educational Resources Information Center

    Cox, James F., III; Walker, Edward D., II

    2005-01-01

    Production planning and control (PPC) systems and operations performance measures are topics that students generally find both boring and difficult to understand. In the article, the authors present a production line game that they have found to be an effective tool to increase student interest in the topics as well as student comprehension. The…

  18. Lithography line productivity impact using Cymer GLX technology

    NASA Astrophysics Data System (ADS)

    O'Brien, Kevin; Dunstan, Wayne; Jacques, Robert; Brown, Daniel

    2009-03-01

    Leading-edge scanners in fabs worldwide have particularly high system utilization and require peak levels of system throughput and availability. Laser gas exchanges typically occur daily on these systems (or every 100M pulses or less), with each exchange lasting up to 20 minutes. This downtime has a direct negative effect on availability, and if it is reduced, the productivity of the litho cell increases. This paper will outline the immediate success fabs have experienced after equipping scanners with Cymer's Gas Lifetime eXtension (GLXTM) technology, which increases scanner availability by extending the time between excimer laser gas exchanges by a factor of more than 10. To date, more than 100 leading-edge scanners feature Cymer's GLX technology, which has improved light source availability by more than 1.5 percent. Moreover, multiple chipmakers report more than 2 percent improvement in litho cell productivity due to GLX, corresponding to 2000 wafers/month increase for a 100,000 wafers/month fab. The increase in measured productivity is the leveraged benefit of reducing process interruptions around the refill cycle GLX technology extends the shot-based interval between gas refills to 1 billion pulses for Cymer's XLA light sources, and provides excellent stability in key optical performance parameters, such as bandwidth and dose stability over the entire gas life. This paper will provide extensive performance data during extended light source operation on litho cells equipped with GLX technology, and multiple use scenarios will be examined, including usage at memory and logic fabs. The paper will also discuss the performance of GLX2TM technology which further extends the maximum time between light source gas exchanges from 1B pulses to 2B pulses, and reduces downtime associated with gas refills by a factor of 20. The stability and productivity benefits of this new technology can be realized under all light source utilization scenarios. With GLX2, the refill

  19. Designing of robotic production lines using CAx software

    NASA Astrophysics Data System (ADS)

    Wróbel, A.; Langer, P.

    2015-11-01

    Present market conditions causes that modern control systems of robotized manufacturing cells should be characterized by the much greater degree of flexibility, selforganization and, above all, adaptability to emerging outer excitations. The phenomenon of information distribution is one of the most important features of modern control systems. In the paper is presented the approach, based on application of multi-agent systems, for supporting the operation of robotized manufacturing cells. The aim of this approach is to obtain the flexible response to outer excitations and preventing situations that might cause the delay of the production process. The presented paper includes description of the concept of an informatics system designed for controlling the work of production systems, including work cells. Such systems could operate independently if it would be equipped with the selforganization mechanism. It is possible in the case of the proposed multi-agent system. The implementation of the presented concept will follow the present analysis of the described concept. The advantage of the proposed concept is its hierarchical depiction that allows integrating different utilized informatics tools in one complex system. It allows preparing the final computer program.

  20. Product-line selection and pricing with remanufacturing under availability constraints

    NASA Astrophysics Data System (ADS)

    Aras, Necati; Esenduran, G.÷k.‡e.; Altinel, I. Kuban

    2004-12-01

    Product line selection and pricing are two crucial decisions for the profitability of a manufacturing firm. Remanufacturing, on the other hand, may be a profitable strategy that captures the remaining value in used products. In this paper we develop a mixed-integer nonlinear programming model form the perspective of an original equipment manufacturer (OEM). The objective of the OEM is to select products to manufacture and remanufacture among a set of given alternatives and simultaneously determine their prices so as to maximize its profit. It is assumed that the probability a customer selects a product is proportional to its utility and inversely proportional to its price. The utility of a product is an increasing function of its perceived quality. In our base model, products are discriminated by their unit production costs and utilities. We also analyze a case where remanufacturing is limited by the available quantity of collected remanufacturable products. We show that the resulting problem is decomposed into the pricing and product line selection subproblems. Pricing problem is solved by a variant of the simplex search procedure which can also handle constraints, while complete enumeration and a genetic algorithm are used for the solution of the product line selection problem. A number of experiments are carried out to identify conditions under which it is economically viable for the firm to sell remanufactured products. We also determine the optimal utility and unit production cost values of a remanufactured product, which maximizes the total profit of the OEM.

  1. Towards automated on-line adaptation of 2-Step IMRT plans: QUASIMODO phantom and prostate cancer cases

    PubMed Central

    2013-01-01

    ) 2-Step generation for the geometry of the day using the relocated isocenter, MU transfer from the planning geometry; 2) Adaptation of the widths of S2 segments to the geometry of the day; 3) Imitation of DMPO fine-tuning for the geometry of the day. Results and conclusion We have performed automated 2-Step IMRT adaptation for ten prostate adaptation cases. The adapted plans show statistically significant improvement of the target coverage and of the rectum sparing compared to those plans in which only the isocenter is relocated. The 2-Step IMRT method may become a core of the automated adaptive radiation therapy system at our department. PMID:24207129

  2. Automated DNA Sequencing System

    SciTech Connect

    Armstrong, G.A.; Ekkebus, C.P.; Hauser, L.J.; Kress, R.L.; Mural, R.J.

    1999-04-25

    Oak Ridge National Laboratory (ORNL) is developing a core DNA sequencing facility to support biological research endeavors at ORNL and to conduct basic sequencing automation research. This facility is novel because its development is based on existing standard biology laboratory equipment; thus, the development process is of interest to the many small laboratories trying to use automation to control costs and increase throughput. Before automation, biology Laboratory personnel purified DNA, completed cycle sequencing, and prepared 96-well sample plates with commercially available hardware designed specifically for each step in the process. Following purification and thermal cycling, an automated sequencing machine was used for the sequencing. A technician handled all movement of the 96-well sample plates between machines. To automate the process, ORNL is adding a CRS Robotics A- 465 arm, ABI 377 sequencing machine, automated centrifuge, automated refrigerator, and possibly an automated SpeedVac. The entire system will be integrated with one central controller that will direct each machine and the robot. The goal of this system is to completely automate the sequencing procedure from bacterial cell samples through ready-to-be-sequenced DNA and ultimately to completed sequence. The system will be flexible and will accommodate different chemistries than existing automated sequencing lines. The system will be expanded in the future to include colony picking and/or actual sequencing. This discrete event, DNA sequencing system will demonstrate that smaller sequencing labs can achieve cost-effective the laboratory grow.

  3. An automated flow injection system for metal determination by flame atomic absorption spectrometry involving on-line fabric disk sorptive extraction technique.

    PubMed

    Anthemidis, A; Kazantzi, V; Samanidou, V; Kabir, A; Furton, K G

    2016-08-15

    A novel flow injection-fabric disk sorptive extraction (FI-FDSE) system was developed for automated determination of trace metals. The platform was based on a minicolumn packed with sol-gel coated fabric media in the form of disks, incorporated into an on-line solid-phase extraction system, coupled with flame atomic absorption spectrometry (FAAS). This configuration provides minor backpressure, resulting in high loading flow rates and shorter analytical cycles. The potentials of this technique were demonstrated for trace lead and cadmium determination in environmental water samples. The applicability of different sol-gel coated FPSE media was investigated. The on-line formed complex of metal with ammonium pyrrolidine dithiocarbamate (APDC) was retained onto the fabric surface and methyl isobutyl ketone (MIBK) was used to elute the analytes prior to atomization. For 90s preconcentration time, enrichment factors of 140 and 38 and detection limits (3σ) of 1.8 and 0.4μgL(-1) were achieved for lead and cadmium determination, respectively, with a sampling frequency of 30h(-1). The accuracy of the proposed method was estimated by analyzing standard reference materials and spiked water samples. PMID:27260436

  4. Automated on-line liquid-liquid extraction system for temporal mass spectrometric analysis of dynamic samples.

    PubMed

    Hsieh, Kai-Ta; Liu, Pei-Han; Urban, Pawel L

    2015-09-24

    Most real samples cannot directly be infused to mass spectrometers because they could contaminate delicate parts of ion source and guides, or cause ion suppression. Conventional sample preparation procedures limit temporal resolution of analysis. We have developed an automated liquid-liquid extraction system that enables unsupervised repetitive treatment of dynamic samples and instantaneous analysis by mass spectrometry (MS). It incorporates inexpensive open-source microcontroller boards (Arduino and Netduino) to guide the extraction and analysis process. Duration of every extraction cycle is 17 min. The system enables monitoring of dynamic processes over many hours. The extracts are automatically transferred to the ion source incorporating a Venturi pump. Operation of the device has been characterized (repeatability, RSD = 15%, n = 20; concentration range for ibuprofen, 0.053-2.000 mM; LOD for ibuprofen, ∼0.005 mM; including extraction and detection). To exemplify its usefulness in real-world applications, we implemented this device in chemical profiling of pharmaceutical formulation dissolution process. Temporal dissolution profiles of commercial ibuprofen and acetaminophen tablets were recorded during 10 h. The extraction-MS datasets were fitted with exponential functions to characterize the rates of release of the main and auxiliary ingredients (e.g. ibuprofen, k = 0.43 ± 0.01 h(-1)). The electronic control unit of this system interacts with the operator via touch screen, internet, voice, and short text messages sent to the mobile phone, which is helpful when launching long-term (e.g. overnight) measurements. Due to these interactive features, the platform brings the concept of the Internet-of-Things (IoT) to the chemistry laboratory environment. PMID:26423626

  5. Active Data Archive Product Tracking and Automated SPASE Metadata Generation in Support of the Heliophysics Data Environment

    NASA Astrophysics Data System (ADS)

    Bargatze, L. F.

    2013-12-01

    The understanding of Solar interaction with the Earth and other bodies in the solar system is a primary goal of Heliophysics as outlined in the NASA Science Mission Directive Science Plan. Heliophysics researchers need access to a vast collection of satellite and ground-based observations coupled with numerical simulation data to study complex processes some of which, as in the case of space weather, pose danger to physical elements of modern society. The infrastructure of the Heliophysics data environment plays a vital role in furthering the understanding of space physics processes by providing researchers with means for data discovery and access. The Heliophysics data environment is highly dynamic with thousands of data products involved. Access to data is facilitated via the Heliophysics Virtual Observatories (VxO) but routine access is possible only if the VxO SPASE metadata repositories contain accurate and up to date information. The Heliophysics Data Consortium has the stated goal of providing routine access to all relevant data products inclusively. Currently, only a small fraction of the data products relevant to Heliophysics studies have been described and registered in a VxO repository. And, for those products that have been described in SPASE, there is a significant time lag from when new data becomes available to when VxO metadata are updated to provide access. It is possible to utilize automated tools to shorten the response time of VxO data product registration via active data archive product tracking. Such a systematic approach is designed to address data access reliability by embracing the highly dynamic nature of the Heliophysics data environment. For example, the CDAWEB data repository located at the NASA Space Science Physics Data facility maintains logs of the data products served to the community. These files include two that pertain to full directory list information, updated daily, and a set of SHA1SUM hash value files, one for each of more

  6. The modular design of robotic workcells in a flexible production line

    NASA Astrophysics Data System (ADS)

    Banas, W.; Sekala, A.; Gwiazda, A.; Foit, K.; Hryniewicz, P.; Kost, G.

    2015-11-01

    In the case of large-scale and mass production lines often the same model of an industrial robot is used in various places of the line and is intended to various task. However, the replacement of one industrial robot to another is a long lasting and arduous process. It requires stopping all the production line and sometimes even dismantling the whole workcell. Such situations are not frequent in production lines that are not flexible. They are related the most often with the failure on an industrial robot. However, during the designing of a flexible production line the ability to replace any robot, which is unrestricted, fast and trouble-free, greatly increase the flexibility level of such line. It could be realized by modular design of the proposed production line. In this way it could be possible to change any elements of such production system. But this approach needs to apply the specialized informatics system. This paper presents the obtained design of several versions of the same production workcell. Each, succeeding version of the designed production workcell contains more and more modular elements. Thereby it would be presented the evolution of a workcell design beginning from the typical design and ending with the fully modular one. One of tools needed to realize this task is the elaboration of a base of modules and typical joint and mounting elements that could be utilised in the described designing process. It is also presented the guidance information about the designing and programming processes useful at each stage of analysed process.

  7. 40 CFR 1051.325 - What happens if an engine family fails the production-line testing requirements?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the production-line testing requirements? 1051.325 Section 1051.325 Protection of Environment... ENGINES AND VEHICLES Testing Production-Line Vehicles and Engines § 1051.325 What happens if an engine family fails the production-line testing requirements? (a) We may suspend your certificate of...

  8. 40 CFR 1054.325 - What happens if an engine family fails the production-line testing requirements?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the production-line testing requirements? 1054.325 Section 1054.325 Protection of Environment... SPARK-IGNITION ENGINES AND EQUIPMENT Production-line Testing § 1054.325 What happens if an engine family fails the production-line testing requirements? (a) We may suspend your certificate of conformity for...

  9. 40 CFR 1042.325 - What happens if an engine family fails the production-line testing requirements?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the production-line testing requirements? 1042.325 Section 1042.325 Protection of Environment... MARINE COMPRESSION-IGNITION ENGINES AND VESSELS Testing Production-line Engines § 1042.325 What happens if an engine family fails the production-line testing requirements? (a) We may suspend...

  10. 40 CFR 1051.310 - How must I select vehicles or engines for production-line testing?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... for production-line testing? 1051.310 Section 1051.310 Protection of Environment ENVIRONMENTAL... VEHICLES Testing Production-Line Vehicles and Engines § 1051.310 How must I select vehicles or engines for production-line testing? (a) Test engines from each engine family as described in this section based on...