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1

Drug carrying hydrogel base wound dressing  

Microsoft Academic Search

A special drug carrying hydrogel base wound dressing by radiation preparation is developed for hospital uses. The dressing possesses high water absorption property. Radiation preparation is carried out using a Van de Graaff Accelerator as an electron radiation source. The effect of absorbed dose and cmount of crosslinking agent on the gel fraction and swelling ratio of the hydrogel were

Jie Chen; Yueqi Yang; Pingbo Qian; Zueteh Ma; Weibin Wu; Peizhi Sung; Xingguo Wang; Jinghui Li

1993-01-01

2

EGF containing gelatin-based wound dressings  

Microsoft Academic Search

In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. In this study, synthesis of a novel polymeric bilayer wound dressing containing

Kezban Ulubayram; A. Nur Cakar; Petek Korkusuz; Cemile Ertan; Nesrin Hasirci

2001-01-01

3

Drug carrying hydrogel base wound dressing  

NASA Astrophysics Data System (ADS)

A special drug carrying hydrogel base wound dressing by radiation preparation is developed for hospital uses. The dressing possesses high water absorption property. Radiation preparation is carried out using a Van de Graaff Accelerator as an electron radiation source. The effect of absorbed dose and cmount of crosslinking agent on the gel fraction and swelling ratio of the hydrogel were determined respectively. As a bio-medical material, standard tests were made. Results showed properties conforming with requirements for clinical applications. Results obtained from clinical tests were good.

Chen, Jie; Yang, Yueqi; Qian, Pingbo; Ma, Zueteh; Wu, Weibin; Sung, Peizhi; Wang, Xingguo; Li, Jinghui

1993-10-01

4

Textile-based smart wound dressings  

Microsoft Academic Search

Efforts have been made during the last few years towards the development of new artificial wound coverings which will meet the requirements necessary for the treatment of major skin wounds. Research has been mainly focused on achieving the specifications of an ideal wound dressing, such as hydrogel technologies providing products suitable for applications in biomedical, personal care as well as

Bhuvanesh Gupta; Roopali Agarwal; M S Alam

2010-01-01

5

Development of two alginate-based wound dressings  

Microsoft Academic Search

Two types of new alginate-based wound dressings, Type-AP and Type-AE, were fabricated by the EDC-activated crosslinking of\\u000a alginate with Polyethyleneimine and Ethylenediamine, respectively. As compared with the commercial non-woven wound dressing,\\u000a Kaltostat®, both Type-AP and Type-AE dressings had higher degradation temperature, lower calcium content, and a sponge-like macroporous\\u000a structure. In addition, these two alginate-based dressings had higher mechanical stress (12.37 ± 1.72

Chih-Tung Chiu; Jui-Sheng Lee; Chi-Shung Chu; Yi-Pin Chang; Yng-Jiin Wang

2008-01-01

6

Development of two alginate-based wound dressings.  

PubMed

Two types of new alginate-based wound dressings, Type-AP and Type-AE, were fabricated by the EDC-activated crosslinking of alginate with Polyethyleneimine and Ethylenediamine, respectively. As compared with the commercial non-woven wound dressing, Kaltostat, both Type-AP and Type-AE dressings had higher degradation temperature, lower calcium content, and a sponge-like macroporous structure. In addition, these two alginate-based dressings had higher mechanical stress (12.37 +/- 1.72 and 6.87 +/- 0.5 MPa for Type-AP and -AE, respectively) and higher water vapor transmission rates (both about 3,500 g/m2/day) than Kaltostat (0.87 +/- 0.12 MPa and 2,538 g/m2/day). Fibroblasts proliferated faster on these two newly developed wound dressings at a higher rate as compared with that on Kalostat dressing. The results of animal study showed that the wounds treated with either Type-AP or Type-AE dressings healed faster than Kaltostat with less encapsulation of residuals by fibrous tissue and more neo-capillary formation. These two newly developed Type-AP and Type-AE porous wound dressings thus have great potential for clinical applications. PMID:18266085

Chiu, Chih-Tung; Lee, Jui-Sheng; Chu, Chi-Shung; Chang, Yi-Pin; Wang, Yng-Jiin

2008-06-01

7

Fibres for wound dressings based on mixed carbohydrate polymer fibres  

Microsoft Academic Search

Polysaccharide-based dressings have increasingly become viable alternatives to somewhat incompatible and often problematic cotton or viscose gauzes traditionally used for wound dressings. Abundant availability of alginates and their relative ease of reversible solubility in particular have been instrumental in their development into fibres and lately their application as vehicles for delivering drugs. This paper reports on the spinning efficiency of

M. Miraftab; Q. Qiao; J. F. Kennedy; S. C. Anand; M. R. Groocock

2003-01-01

8

Wound dressing based on nonwoven viscose fabrics.  

PubMed

Nonwoven viscose fabric was treated with chitosan/polyvinyl alcohol (PVA) using pad-dry method, using different concentrations of chitosan and PVA. Increasing the amount of PVA leads to increasing of air permeability. Water permeability increased by increasing the amount of PVA to 2 ml (10% solution) then decreased by any increase of the quantity of PVA solution. Roughness increased with increasing the amount of 10% PVA solution. It is shown that roughness, water and air permeability increased with increasing the chitosan concentration. Antibacterial properties was increased with increasing PVA/or chitosan concentration. The chitosan/PVA treated nonwoven viscose fabric was immersed in a solution of Ag nanoparticles. The chitosan/PVA/Ag nanoparticles treated nonwoven fabrics were used as wound dressings on French white Bouscat rabbits, with age ranged from 1 to 2 years. A complete healing was achieved using wound dressing consists of nonwoven viscose fabric treated with chitosan/PVA/Ag nanoparticles after 21 days. The histopathological examination confirmed the complete re-epithelialization and averagely thick epidermis formation. PMID:24751090

Abou-Okeil, A; Sheta, A M; Amr, A; Ali, Marwa A

2012-09-01

9

Biomaterials based on chitin and chitosan in wound dressing applications.  

PubMed

Wound dressing is one of the most promising medical applications for chitin and chitosan. The adhesive nature of chitin and chitosan, together with their antifungal and bactericidal character, and their permeability to oxygen, is a very important property associated with the treatment of wounds and burns. Different derivatives of chitin and chitosan have been prepared for this purpose in the form of hydrogels, fibers, membranes, scaffolds and sponges. The purpose of this review is to take a closer look on the wound dressing applications of biomaterials based on chitin, chitosan and their derivatives in various forms in detail. PMID:21262336

Jayakumar, R; Prabaharan, M; Sudheesh Kumar, P T; Nair, S V; Tamura, H

2011-01-01

10

Testing Intelligent Wound Dressings  

Microsoft Academic Search

While occlusive wound dressings help provide patients with moist wound healing to reduce pain and increase reepithelialization rate, the moisture vapor transmission rate (MVTR) of these dressings remains constant even though wound exudate levels may vary with time and from wound to wound. The clinician is therefore faced with exudate buildup in heavily exuding wounds and desiccation in lightly exuding

Shashi Palamand; Andrew M. Reed; Ludwig J. Weimann

1992-01-01

11

Fatty acid-based polyurethane films for wound dressing applications  

Microsoft Academic Search

Fatty acid-based polyurethane films were prepared for use as potential wound dressing material. The polymerization reaction\\u000a was carried out with or without catalyst. Polymer films were prepared by casting-evaporation technique with or without crosslink-catalyst.\\u000a The film prepared from uncatalyzed reaction product with crosslink-catalyst gave slightly higher crosslink density. The mechanical\\u000a tests showed that, the increase in the tensile strength and

Guncem Gultekin; Cigdem Atalay-Oral; Sibel Erkal; Fikret Sahin; Djursun Karastova; S. Birgul Tantekin-Ersolmaz; F. Seniha Guner

2009-01-01

12

Fatty acid-based polyurethane films for wound dressing applications  

Microsoft Academic Search

Fatty acid-based polyurethane films were prepared for use as potential wound dressing material. The polymerization reaction was carried out with or without catalyst. Polymer films were prepared by casting-evaporation technique with or without crosslink-catalyst. The film prepared from uncatalyzed reaction product with crosslink-catalyst gave slightly higher crosslink density. The mechanical tests showed that, the increase in the tensile strength and

G. Gultekin; C. Ataly-Oral; S. Erkai; F. Sahin; D. Karastova; S. Tatntekin-Ersolmaz; F. Guner

2008-01-01

13

beta-Chitin-based wound dressing containing silver sulfurdiazine.  

PubMed

Physical and biological properties of some wound dressing materials based on beta-chitin were studied. Water vapor transmission rates (WVTR), oxygen permeabilities and biodegradation kinetics were examined for film-type samples. WVTR of samples was in the range 2400-2800 g/m2/day. However, oxygen permeabilities of the samples were relatively low. To improve oxygen permeabilities, porous sponge-type wound dressing materials were prepared. In addition, these sponge-type samples contained antimicrobial agents, silver sulfadiazine (AgSD), in order to prevent bacteria infection on a wound surface. Anti-microbacterial tests on agar plate were carried out to confirm the bactericidal capacity of present materials. These materials impregnating AgSD had the complete bactericidal capacity against pseudomonas aeruginosa up to 7 days. Finally, a wound healing effect of beta-chitin-based semi-interpenetrating polymer networks was evaluated from the animal test using the wistar rat in vivo. Histological studies confirm the proliferation of fibroblasts in the wound bed and a distinct reduction in infectious cells. PMID:15348066

Lee, Y M; Kim, S S; Park, M H; Song, K W; Sung, Y K; Kang, I K

2000-12-01

14

[Wound dressings in chronic wounds].  

PubMed

Hydroactive wound dressings retain exsudate in the wound region or incorporate wound exsudate by gel formation. They create the local environment for moist wound healing which is experimentally and clinically characterized by accelerated reepithelialization, inflammatory reaction and angiogenesis as well as reduced wound pain and wound infection rates. Clinically relevant product groups of hydroactive wound dressings (hydrocolloids and hydropolymers, semipermeable films, calcium alginates) are distinct as to chemical structure, physical properties and functional characteristics in local wound treatment. Between the product groups, there are considerable differences with respect to inflammatory reactions at the wound bottom, absorption of exsudate, occlusion properties, wound edge adherence, adaptability to the wound shape and material integrity of wound dressings. Experimental and clinical results of moist wound treatment by hydroactive wound dressings such as hydrocolloids and hydropolymers, semipermeable films or calcium alginates reveal a wide range of local response on the different types of dressings. They offer the opportunity of therapeutic differentiation. To elucidate the differential indication for different product groups of hydroactive wound dressings in local treatment of chronic wounds, additional experimental and clinical research is required. PMID:10436530

Mohr, V D; Spelter, H; Schmidt, J; Zirngibl, H

1999-01-01

15

In Vivo Performance of Chitosan/Soy-Based Membranes as Wound-Dressing Devices for Acute Skin Wounds  

PubMed Central

Wound management represents a major clinical challenge on what concerns healing enhancement and pain control. The selection of an appropriate dressing plays an important role in both recovery and esthetic appearance of the regenerated tissue. Despite the wide range of available dressings, the progress in the wound care market relies on the increasing interest in using natural-based biomedical products. Herein, a rat wound-dressing model of partial-thickness skin wounds was used to study newly developed chitosan/soy (cht/soy)-based membranes as wound-dressing materials. Healing and repair of nondressed, cht/soy membrane-dressed, and Epigard®-dressed wounds were followed macroscopically and histologically for 1 and 2 weeks. cht/soy membranes performed better than the controls, promoting a faster wound repair. Re-epithelialization, observed 1 week after wounding, was followed by cornification of the outermost epidermal layer at the second week of dressing, indicating repair of the wounded tissue. The use of this rodent model, although in impaired healing conditions, may enclose some drawbacks regarding the inevitable wound contraction. Moreover, being the main purpose the evaluation of cht/soy-based membranes' performance in the absence of growth factors, the choice of a clinically relevant positive control was limited to a polymeric mesh, without any growth factor influencing skin healing/repair, Epigard. These new cht/soy membranes possess the desired features regarding healing/repair stimulation, ease of handling, and final esthetic appearance—thus, valuable properties for wound dressings.

Santos, Tircia C.; Horing, Bernhard; Reise, Kathrin; Marques, Alexandra P.; Silva, Simone S.; Oliveira, Joaquim M.; Mano, Joao F.; Castro, Antonio G.; van Griensven, Martijn

2013-01-01

16

Biomaterials based on chitin and chitosan in wound dressing applications  

Microsoft Academic Search

Wound dressing is one of the most promising medical applications for chitin and chitosan. The adhesive nature of chitin and chitosan, together with their antifungal and bactericidal character, and their permeability to oxygen, is a very important property associated with the treatment of wounds and burns. Different derivatives of chitin and chitosan have been prepared for this purpose in the

R. Jayakumar; M. Prabaharan; P. T. Sudheesh Kumar; S. V. Nair; H. Tamura

2011-01-01

17

Advances in wound dressings.  

PubMed

Wound dressings have undergone an evolutionary process from natural materials that simply covered and concealed the wound, to materials that focused on moisture management, and more recently, to materials that either deliver active ingredients or interact directly with cells or specific chemicals in the local wound environment. Advances in dressings technology have led to a new proliferation of topical products that do more than just cover and conceal, but that also can facilitate the healing process as well as address specific issues in nonhealing wounds. Dressings may play an important adjunctive role in concert with overall efforts to manage the underlying causes of chronic nonhealing wounds. PMID:17276199

Ovington, Liza G

2007-01-01

18

Fatty acid-based polyurethane films for wound dressing applications.  

PubMed

Fatty acid-based polyurethane films were prepared for use as potential wound dressing material. The polymerization reaction was carried out with or without catalyst. Polymer films were prepared by casting-evaporation technique with or without crosslink-catalyst. The film prepared from uncatalyzed reaction product with crosslink-catalyst gave slightly higher crosslink density. The mechanical tests showed that, the increase in the tensile strength and decrease in the elongation at break is due to the increase in the degree of crosslinking. All films were flexible, and resisted to acid solution. The films prepared without crosslink-catalyst were more hydrophilic, absorbed more water. The highest permeability values were generally obtained for the films prepared without crosslink catalyst. Both the direct contact method and the MMT test were applied for determination of cytotoxicity of polymer films and the polyurethane film prepared from uncatalyzed reaction product without crosslink-catalyst showed better biocompatibility property, closest to the commercial product, Opsite. PMID:18839285

Gultekin, Guncem; Atalay-Oral, Cigdem; Erkal, Sibel; Sahin, Fikret; Karastova, Djursun; Tantekin-Ersolmaz, S Birgul; Guner, F Seniha

2009-01-01

19

Wound healing evaluation of sodium fucidate-loaded polyvinylalcohol/sodium carboxymethylcellulose-based wound dressing.  

PubMed

The cross-linked hydrogel films containing sodium fucidate were previously reported to be prepared polyvinyl alcohol (PVA) and sodium carboxymethylcellulose (Na-CMC) using the freeze-thawing method and their physicochemical property was investigated. For the development of novel sodium fucidate-loaded wound dressing, here its in vivo wound healing test and histopathology were performed compared with the conventional ointment product. In wound healing test, the sodium fucidate-loaded composed of 2.5% PVA, 1.125% Na-CMC and 0.2% drug showed faster healing of the wound made in rat dorsum than the hydrogel without drug, indicating the potential healing effect of sodium fucidate. Furthermore, from the histological examination, the healing effect of sodium fucidate-loaded hydrogel was greater than that of the conventional ointment product and hydrogel without drug, since it might gave an adequate level of moisture and build up the exudates on the wound area. Thus, the sodium fucidate-loaded wound dressing composed of 5% PVA, 1.125% Na-CMC and 0.2% drug is a potential wound dressing with excellent wound healing. PMID:20661719

Lee, Jeong Hoon; Lim, Soo-Jeong; Oh, Dong Hoon; Ku, Sae Kwang; Li, Dong Xun; Yong, Chul Soon; Choi, Han-Gon

2010-07-01

20

Preparation and characterization of antimicrobial wound dressings based on silver, gellan, PVA and borax.  

PubMed

Silver-loaded dressings are designed to provide the same antimicrobial activity of topical silver, with the advantages of a sustained silver release and a reduced number of dressing changes. Moreover, such type of dressing must provide a moist environment, avoiding fiber shedding, dehydration and adherence to the wound site. Here we describe the preparation of a novel silver-loaded dressing based on a Gellan/Hyaff(®) (Ge-H) non woven, treated with a polyvinyl alcohol (PVA)/borax system capable to enhance the entrapment of silver in the dressing and to modulate its release. The new hydrophilic non woven dressings show enhanced water uptake capability and slow dehydration rates. A sustained silver release is also achieved. The antibacterial activity was confirmed on Staphylococcus aureus and Pseudomonas aeruginosa. PMID:22939352

Cencetti, C; Bellini, D; Pavesio, A; Senigaglia, D; Passariello, C; Virga, A; Matricardi, P

2012-10-15

21

Antimicrobial Wound Dressing. Phase 1.  

National Technical Information Service (NTIS)

The main objective of Phase I of this SBIR project was to demonstrate the feasibility for successful development of an antimicrobial wound dressing based on a hydrogel sustained release matrix. This goal has been achieved. In vitro studies of drug diffusi...

M. H. Gay E. S. Nuwayser P. D. Blaskovich D. L. Mcafee

1987-01-01

22

Evaluation of an in situ forming hydrogel wound dressing based on oxidized alginate and gelatin  

Microsoft Academic Search

Wound dressings that can be formed in situ offer several advantages over the use of preformed dressings such as conformability without wrinkling or fluting in the wound bed, ease of application and improved patient compliance and comfort. Here we describe such an in situ forming hydrogel wound dressing from gelatin, oxidized alginate and borax. Periodate oxidized alginate rapidly cross-links proteins

Biji Balakrishnan; M. Mohanty; P. R. Umashankar; A. Jayakrishnan

2005-01-01

23

Biocompatible wound dressings based on chemically degradable triblock copolymer hydrogels.  

PubMed

The synthesis of a series of thermo-responsive ABA triblock copolymers in which the outer A blocks comprise poly(2-hydroxypropyl methacrylate) and the central B block is poly(2-(methacryloyloxy)ethyl phosphorylcholine) is achieved using atom transfer radical polymerization. These novel triblock copolymers form thermo-reversible physical gels with critical gelation temperatures and mechanical properties that are highly dependent on the copolymer composition and concentration. TEM studies on dried dilute copolymer solutions indicate the presence of colloidal aggregates, which is consistent with micellar gel structures. This hypothesis is consistent with the observation that incorporating a central disulfide bond within the B block leads to thermo-responsive gels that can be efficiently degraded using mild reductants such as dithiothreitol (DTT) over time scales of minutes at 37 degrees C. Moreover, the rate of gel dissolution increases at higher DTT/disulfide molar ratios. Finally, these copolymer gels are shown to be highly biocompatible. Only a modest reduction in proliferation was observed for monolayers of primary human dermal fibroblasts, with no evidence for cytotoxicity. Moreover, when placed directly on 3D tissue-engineered skin, these gels had no significant effect on cell viability. Thus, we suggest that these thermo-responsive biodegradable copolymer gels may have potential applications as wound dressings. PMID:18598077

Madsen, Jeppe; Armes, Steven P; Bertal, Karima; Lomas, Hannah; Macneil, Sheila; Lewis, Andrew L

2008-08-01

24

[Dressing and wound care pain].  

PubMed

Wound care is an important step for promoting wound healing. Nevertheless it is also a major source of pain for patients with wounds. The results of a survey showed that not only burn patients but also non-burn ones suffered from wound care pain which occurred in inpatients and outpatients. One of the significant factors causing wound care pain was that the dressing adhered to the wound bed. Although some agencies claimed that particular dressings with low adhesion can result in painless removal, the actual effects needed to be verified. Results of clinical trials revealed that for relieving wound care pain of certain kinds of wound, it was recommended to use particular dressings, such as banana leaf dressing, boiled potato peel bandage, Acticoat, Mepital or Mefix. PMID:17160873

Chin, Yen-Fan

2006-12-01

25

Electrospun chitosan-based nanocomposite mats reinforced with chitin nanocrystals for wound dressing.  

PubMed

The aim of this study was to develop electrospun chitosan/polyethylene oxide-based randomly oriented fiber mats reinforced with chitin nanocrystals (ChNC) for wound dressing. Microscopy studies showed porous mats of smooth and beadless fibers with diameters between 223 and 966nm. The addition of chitin nanocrystals as well as crosslinking had a positive impact on the mechanical properties of the mats, and the crosslinked nanocomposite mats with a tensile strength of 64.9MPa and modulus of 10.2GPa were considered the best candidate for wound dressing application. The high surface area of the mats (35m(2)g(-1)) was also considered beneficial for wound healing. The water vapor transmission rate of the prepared mats was between 1290 and 1548gm(-2)day(-1), and was in the range for injured skin or wounds. The electrospun fiber mats showed compatibility toward adipose derived stem cells, further confirming their potential use as wound dressing materials. PMID:24815394

Naseri, Narges; Algan, Constance; Jacobs, Valencia; John, Maya; Oksman, Kristiina; Mathew, Aji P

2014-08-30

26

Dressing wounds with potato peel.  

PubMed

The use of boiled potato peel (PP) in dressing of various skin conditions was studied. A total of 11 patients were selected, which included resistant wounds of pemphigus, bullous pemphigoid and leg ulcers. An autoclaved PP dressing with a thin layer of antiseptic cream was applied at 25 sites. It was covered with multilayered gauze and the dressing was secured firmly with either a roller bandage or with an adhesive tape. Complete epithelization was seen at 20 sites (80%), near complete epithelization at one site. There was no satisfactory response at three sites and at one site the result could not be evaluated. The mean duration of healing was one week for superficial wounds and three weeks for deep wounds. The PP dressing facilitates the wound and three weeks for deep wounds. The PP dressing facilitates the wound healing process by providing and maintaining a moist environment. The PP dressing is easy to prepare, apply as well as remove. It is a comfortable dressing and is also cost effective. PMID:20948091

Patange, V S; Fernandez, R J; Motla, M U; Mahajan, S A

1996-01-01

27

A new carbohydrate-based wound dressing fibre with superior absorption and antimicrobial potency.  

PubMed

Heavily exudating wounds can lead to infection and unnecessary trauma if they are not adequately managed. Manufacturers involved in production and marketing of high absorption silver dressings, besides emphasising high absorptions of their dressings are keen to highlight potent antimicrobial abilities of their products against all kinds of pathogens including MRSA. However, there are little or no credible reports on minimal but potent quantities of silver needed in a dressing to eliminate bacteria spread and growth or how effectively the silver within a dressing is released over time. This paper introduces a new hybrid biomaterial fibre made from polysaccharide-based polymers with inbuilt ability to gel and absorb large quantities of pseudo exudates. Furthermore, it will be reported that the new fibre carries up to six times less silver than it is conventionally used in silver dressings and displays a very slow rate of release whilst maintaining full potency over time against known Gram positive, Gram negative micro-organisms including MRSA. The paper concludes that the developed hybrid fibre has long lasting antimicrobial and gelling properties comparable, if not better, than Acticoat AA and Aquacel Ag, two commercially available silver dressings. PMID:24299890

Miraftab, M; Masood, R; Edward-Jones, V

2014-01-30

28

Superabsorbent polysaccharide hydrogels based on pullulan derivate as antibacterial release wound dressing.  

PubMed

To accomplish ideal wound dressing, hydrogels based on a natural polysaccharide, pullulan were synthesized by chemical cross-linking. The tensile strengths of the hydrogel films (1 mm thick) were determined to range from 0.663 to 1.097 MPa in proportion to cross-linking degrees and water contents. The swelling study of the hydrogels in water showed remarkable water absorption property with swelling ratio up to 4000%, which provided the hydrogel with quick hemostatic ability and prevent the wound bed from accumulation of exudates. The water vapor transmission rate and water retention of the hydrogels were found to be in the range of 2213-3498 g/m²/day and 34.74-45.81% (after 6 days), indicating that the hydrogel can maintain a moist environment over wound bed, which could prevent the dehydration of the wound bed and prevent the scab formation. Biocompatibility test revealed that the hydrogels were not cytotoxic. The hydrogel could load antimicrobial agents and effectively suppress bacterial proliferation to protect the wound from bacterial invasion. These results suggest that the pullulan hydrogels prepared in this study may have high potential as new ideal wound-dressing materials. PMID:21523902

Li, Huanan; Yang, Jing; Hu, Xiaona; Liang, Jie; Fan, Yujiang; Zhang, Xingdong

2011-07-01

29

Wound Management: The Occlusive Dressing  

PubMed Central

Superficial wounds resulting from athletic injury are common in sports medicine. Although such wounds can be quite painful, they are usually merely inconvenient to the athlete. If improperly managed, however, superficial wounds may heal slowly and cause unnecessary scar tissue proliferation at the wound site. Scar formation causes the wound to break open frequently and puts the athlete at risk of cross-contamination by pathogenic organisms. New advances in the science of wound management strongly favor the use of occlusive dressings to increase patient comfort, increase patient compliance, decrease the risk of infection, and decrease overall healing time. Occlusion has clearly been proven to aid in the healing of superficial wounds and should be considered as a treatment alternative for wounds in the sports medicine setting. In this paper, I discuss three of the most widely used types of occlusive dressings: 1) films, 2) hydrogels, and 3) hydrocolloids.

Rheinecker, Scot B.

1995-01-01

30

Development of polyvinyl alcohol-sodium alginate gel-matrix-based wound dressing system containing nitrofurazone.  

PubMed

Polyvinyl alcohol (PVA)/sodium alginate (SA) hydrogel matrix-based wound dressing systems containing nitrofurazone (NFZ), a topical anti-infective drug, were developed using freeze-thawing method. Aqueous solutions of nitrofurazone and PVA/SA mixtures in different weight ratios were mixed homogeneously, placed in petri dishes, freezed at -20 degrees C for 18h and thawed at room temperature for 6h, for three consecutive cycles, and evaluated for swelling ratio, tensile strength, elongation and thermal stability of the hydrogel. Furthermore, the drug release from this nitrofurazone-loaded hydrogel, in vitro protein adsorption test and in vivo wound healing observations in rats were performed. Increased SA concentration decreased the gelation%, maximum strength and break elongation, but it resulted into an increment in the swelling ability, elasticity and thermal stability of hydrogel film. However, SA had insignificant effect on the release of nitrofurazone. The amounts of proteins adsorbed on hydrogel were increased with increasing sodium alginate ratio, indicating the reduced blood compatibility. In vivo experiments showed that this hydrogel improved the healing rate of artificial wounds in rats. Thus, PVA/SA hydrogel matrix based wound dressing systems containing nitrofurazone could be a novel approach in wound care. PMID:18440737

Kim, Jong Oh; Park, Jung Kil; Kim, Jeong Hoon; Jin, Sung Giu; Yong, Chul Soon; Li, Dong Xun; Choi, Jun Young; Woo, Jong Soo; Yoo, Bong Kyu; Lyoo, Won Seok; Kim, Jung-Ae; Choi, Han-Gon

2008-07-01

31

CCMR: Wound Dressing Tool and Wound Analysis  

NSDL National Science Digital Library

The goal of our project is to develop a Wound Dressing Tool (WDT) that in addition to extracting overabundant chemicals like the VAC system does, can also allow for variable rates of mass transfer as well as a way for clinicians to monitor the fluid chemical composition of the wound bed during the healing and treatment processes.

Men, Shannon

2005-08-17

32

Evaluation of an in situ forming hydrogel wound dressing based on oxidized alginate and gelatin.  

PubMed

Wound dressings that can be formed in situ offer several advantages over the use of preformed dressings such as conformability without wrinkling or fluting in the wound bed, ease of application and improved patient compliance and comfort. Here we describe such an in situ forming hydrogel wound dressing from gelatin, oxidized alginate and borax. Periodate oxidized alginate rapidly cross-links proteins such as gelatin in the presence of borax to give in situ forming hydrogels that are both non-toxic and biodegradable. The composite matrix has the haemostatic effect of gelatin, the wound healing-promoting feature of alginate and the antiseptic property of borax to make it a potential wound dressing material. The hydrogel was found to have a fluid uptake of 90% of its weight which would prevent the wound bed from accumulation of exudates. The water vapour transmission rate (WVTR) of the hydrogel was found to be 2686+/-124 g/m2/day indicating that the hydrogel can maintain a moist environment over wound bed in moderate to heavily exuding wound which would enhance epithelial cell migration during the healing process. The wound healing efficacy of hydrogel was evaluated in experimental full thickness wounds using a rat model which demonstrated that within 2 weeks, the wound covered with gel was completely filled with new epithelium without any significant adverse reactions. These in situ forming hydrogels fulfil many critical elements desirable in a wound dressing material. PMID:15919113

Balakrishnan, Biji; Mohanty, M; Umashankar, P R; Jayakrishnan, A

2005-11-01

33

Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties  

Microsoft Academic Search

Hemorrhage remains a leading cause of early death after trauma, and infectious complications in combat wounds continue to challenge caregivers. Although chitosan dressings have been developed to address these problems, they are not always effective in controlling bleeding or killing bacteria. We aimed to refine the chitosan dressing by incorporating a procoagulant (polyphosphate) and an antimicrobial (silver). Chitosan containing different

Shin-Yeu Ong; Jian Wu; Shabbir M. Moochhala; Mui-Hong Tan; Jia Lu

2008-01-01

34

Preparation of Transparent Starch Based Hydrogel Membrane with Potential Application as Wound Dressing  

Microsoft Academic Search

In the process of wound repair, a biological environment, numerous cellular processes are interlinked. Modern dressings increase the wound-healing rate rather than just to cover it. Hydrogel dressing can protect injured skin and keep it appropriately moist to speed the healing process. The design and development of novel membrane of hydrogels prepared by crosslinking of polyvinyl alcohol with heat-treated corn-starch

Kunal Pal; A. K. Banthia; D. K. Majumdar

35

Active wound dressings based on bacterial nanocellulose as drug delivery system for octenidine.  

PubMed

Although bacterial nanocellulose (BNC) may serve as an ideal wound dressing, it exhibits no antibacterial properties by itself. Therefore, in the present study BNC was functionalized with the antiseptic drug octenidine. Drug loading and release, mechanical characteristics, biocompatibility, and antimicrobial efficacy were investigated. Octenidine release was based on diffusion and swelling according to the Ritger-Peppas equation and characterized by a time dependent biphasic release profile, with a rapid release in the first 8h, followed by a slower release rate up to 96h. The comparison between lab-scale and up-scale BNC identified thickness, water content, and the surface area to volume ratio as parameters which have an impact on the control of the release characteristics. Compression and tensile strength remained unchanged upon incorporation of octenidine in BNC. In biological assays, drug-loaded BNC demonstrated high biocompatibility in human keratinocytes and antimicrobial activity against Staphylococcus aureus. In a long-term storage test, the octenidine loaded in BNC was found to be stable, releasable, and biologically active over a period of 6 months without changes. In conclusion, octenidine loaded BNC presents a ready-to-use wound dressing for the treatment of infected wounds that can be stored over 6 months without losing its antibacterial activity. PMID:24792978

Moritz, Sebastian; Wiegand, Cornelia; Wesarg, Falko; Hessler, Nadine; Müller, Frank A; Kralisch, Dana; Hipler, Uta-Christina; Fischer, Dagmar

2014-08-25

36

Alginate based bilayer hydrocolloid films as potential slow-release modern wound dressing.  

PubMed

The aims of this research were to develop a novel bilayer hydrocolloid film based on alginate and to investigate its potential as slow-release wound healing vehicle. The bilayer is composed of an upper layer impregnated with model drug (ibuprofen) and a drug-free lower layer, which acted as a rate-controlling membrane. The thickness uniformity, solvent loss, moisture vapour transmission rate (MVTR), hydration rate, morphology, rheology, mechanical properties, in vitro drug release and in vivo wound healing profiles were investigated. A smooth bilayer film with two homogenous distinct layers was produced. The characterisation results showed that bilayer has superior mechanical and rheological properties than the single layer films. The bilayers also showed low MVTR, slower hydration rate and lower drug flux in vitro compared to single layer inferring that bilayer may be useful for treating low suppurating wounds and suitable for slow release application on wound surfaces. The bilayers also provided a significant higher healing rate in vivo, with well-formed epidermis with faster granulation tissue formation when compared to the controls. In conclusions, a novel alginate-based bilayer hydrocolloid film was developed and results suggested that they can be exploited as slow-release wound dressings. PMID:22643226

Thu, Hnin-Ei; Zulfakar, Mohd Hanif; Ng, Shiow-Fern

2012-09-15

37

Wound Dressing in Maxillofacial Trauma.  

National Technical Information Service (NTIS)

Biodegradable polymers of poly-L(-)lactide (R.S.V. = 0.3 and 1.3 dl/g), poly-DL-lactide and poly(lactide-co-glycolide) were prepared and blended with drugs for use in wound dressings. Three local anesthetics (procaine, benzocaine, and etidocaine), two ant...

D. L. Williams B. W. Tenney J. J. Dillon

1979-01-01

38

Development of An Ultra-Fast-Curing Wound Dressing.  

National Technical Information Service (NTIS)

We are developing a drug dispensing field wound dressing. The wound dressing, which can be easily applied by an untrained person, contains a coagulant to stop bleeding, and an antibiotic to prevent bacterial infection. The medicated wound dressing is made...

M. Szycher J. L. Rolfe

1987-01-01

39

21 CFR 878.4022 - Hydrogel wound dressing and burn dressing.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Hydrogel wound dressing and burn dressing. 878.4022 Section 878.4022 Food and Drugs...Surgical Devices § 878.4022 Hydrogel wound dressing and burn dressing. (a) Identification....

2010-04-01

40

21 CFR 878.4022 - Hydrogel wound dressing and burn dressing.  

Code of Federal Regulations, 2010 CFR

...2009-04-01 2009-04-01 false Hydrogel wound dressing and burn dressing. 878.4022 Section 878.4022 Food and Drugs...Surgical Devices § 878.4022 Hydrogel wound dressing and burn dressing. (a) Identification....

2009-04-01

41

Drug loaded thermoresponsive and cytocompatible chitosan based hydrogel as a potential wound dressing  

Microsoft Academic Search

There is a demand for a wound dressing which can be removed from the application site easily without causing any pain or discomfort. A material removable by manipulating the temperature (e.g. moisten with ice cold water), from that point of view, seems to have considerable potential. Here we report a new formulation consisting of thiolated chitosan with poly(N-isopropyl acrylamide) loaded

C. Radhakumary; Molly Antonty; K. Sreenivasan

2011-01-01

42

[Moist wound treatment with ointment dressings in surface skin defects].  

PubMed

The advantages of moist wound treatment by means of ointment gauze dressing are based on the following attributes of the marked tulle gras preparations: --easy to handle; storage without problem --the gauze dressings can be modeled easily even on difficult body contoures (for example in the face) --the gauze dressing can be changed easily --Synthetic tulle gras dressings do not fray so that no residues remain in the wound. Therefore the danger of adhesion with the wound can be minimized. --There is no congestion of secretions undermath the gauze dressing. --undisturbed healing: a presupposing factor for optimal cosmetic and therapeutic result. --Minimal danger of contact dermatitis by using paraffin gauze dressing without antiseptic additives. PMID:3073599

Petres, J

1988-01-01

43

Evaluation of bacterial nanocellulose-based uniform wound dressing for large area skin transplantation.  

PubMed

Bacterial nanocellulose (BNC) was biosynthesized by Gluconacetobacter xylinus. The surface area, physicochemical structure and morphology of the materials were characterized. Here provides a method for an efficient production of uniform BNC, which is beneficial for the fast characterization and evaluation of BNC. In vitro cytotoxicity of the materials was evaluated by the proliferation, the adhesion, the viability and the morphology of NIH/3T3 cells. Low cytotoxicity of the BNC was observed, and micrographs demonstrate a good proliferation and adhesion of NIH/3T3 cells on BNC. Large area full-thickness skin defects were made on the back of C57BL/6 mice in animal surgery. The wounds were transplanted with BNC films and the results compared to those in a control group. The rehabilitation of the wound surfaces and the pathological sections of mice were investigated and are discussed. Histological examinations demonstrated faster and better healing effect and lower inflammatory response in the BNC group than those in the control group. Preliminary results on wound dressings from BNC show a curative effect promoting the healing of epithelial tissue. BNC is a promising natural polymer with medical applications in wound dressings. PMID:23623124

Fu, Lina; Zhou, Ping; Zhang, Shengmin; Yang, Guang

2013-07-01

44

Transmission of Therapeutic Ultrasound by Wound Dressings  

Microsoft Academic Search

Ultrasound has been used for the treatment of a variety of cutaneous wounds, particularly venous ulcers. Many of the published studies involved application of ultrasound to the surrounding tissue rather than directly over the wound. Insonating the wound itself may enhance the healing process, but the lack of data regarding the trans- mission characteristics of dressings has limited the use

Leon Poltawski; Tim Watson

45

Easily Applied Long Lasting Dressing Based on POROPLASTIC (Trade Name) Materials. A Long Lasting Controlled-Release Wound Dressing Based on POROPLASTIC Membrane, Delivering Clindamycin and Gentamicin.  

National Technical Information Service (NTIS)

This report is the result of an opportunity. The opportunity brought together consumer need with a promising technical advance. The U.S. Army Institute of Dental Research (USAIDR) formulated the need for a wound dressing able to inhibit the development of...

S. J. Davidson S. Obermayer D. Nichols N. Hurd B. Allen

1988-01-01

46

Polyox and carrageenan based composite film dressing containing anti-microbial and anti-inflammatory drugs for effective wound healing.  

PubMed

Polyethylene oxide (Polyox) and carrageenan based solvent cast films have been formulated as dressings for drug delivery to wounds. Films plasticised with glycerol were loaded with streptomycin (30%, w/w) and diclofenac (10%, w/w) for enhanced healing effects in chronic wounds. Blank and drug loaded films were characterised by texture analysis (for mechanical and mucoadhesive properties), scanning electron microscopy, differential scanning calorimetry, X-ray diffraction and Fourier transform infrared spectroscopy. In addition, swelling, in vitro drug release and antibacterial studies were conducted to further characterise the films. Both blank and drug loaded films showed a smooth, homogeneous surface morphology, excellent transparency, high elasticity and acceptable tensile (mechanical) properties. The drug loaded films showed a high capacity to absorb simulated wound fluid and significant mucoadhesion force which is expected to allow effective adherence to and protection of the wound. The films showed controlled release of both streptomycin and diclofenac for 72 h. These drug loaded films produced higher zones of inhibition against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli compared to the individual drugs zones of inhibition. Incorporation of streptomycin can prevent and treat chronic wound infections whereas diclofenac can target the inflammatory phase of wound healing to relieve pain and swelling. PMID:23228898

Boateng, Joshua S; Pawar, Harshavardhan V; Tetteh, John

2013-01-30

47

Toxicity assessment of nanosilver wound dressing in Wistar rat.  

PubMed

Antibiotic resistance to microorganisms is one of the major problems faced in the field of wound care in burns patients. Silver nanoparticles have come up as potent antimicrobial agent and are being evaluated in diverse medical applications ranging from silver based dressings to silver coated medical devices. We aimed in present study to test the release of nanosilver from nanosilver wound dressing and compare the dermal and systemic toxicity of nanosilver dressings in a repeated dose (21 days) model. Under general anesthesia, a limited standard 2nd degree burns were provided on the back of each rat in all treatment, negative control (simple dressing) and 5% silver nitrate groups, each contained 5 male wistar rats. According to the analysis made by atomic absorption spectrometry, the wound dressings released 0.599 ± 0.083 ppm of nanosilver during first 24 hrs of study. Daily observations were recoded and wounds were covered with new dressings each 24 hrs. Burn healing was observed in nanosilver wound dressing group in shorter time periods than the control groups. In toxicity assessment, this dressing didn't cause any hematological and histopathological abnormalities in treatment group but biochemical studies showed significant rise of plasma transaminase (ALT) at the endpoint (21 days) of the study (P=0.027). Portal mononuclear lymphoid and polymorphonuclear leukocyte infiltrations in three to four adjacent foci were recognized around the central hepatic vein in treatment group. Mild hepatotoxic effects of nanosilver wound dressing in wistar rat emphasize the necessity of more studies on toxicity potentials of low dose nanosilver by dermal applications. PMID:23690097

Bidgoli, Sepideh Arbabi; Mahdavi, Moujan; Rezayat, Seyed Mahdi; Korani, Mitra; Amani, Amir; Ziarati, Parisa

2013-01-01

48

PVA–clay nanocomposite hydrogels for wound dressing  

Microsoft Academic Search

Polymer gels as soft biomaterials have found diverse applications in biomedical field, e.g. in management and care of wound as wound dressing.The recent researches on nanocomposite materials have shown that some properties of polymers and gels significantly improve by adding organoclay into polymeric matrix. In this work, in order to obtain wound dressing with better properties, nanocomposite hydrogel wound dressing

Mehrdad Kokabi; Mohammad Sirousazar; Zuhair Muhammad Hassan

2007-01-01

49

In vivo tests of a novel wound dressing based on biomaterials with tissue adhesion controlled through external stimuli  

Microsoft Academic Search

The high incidence of wounds by second intention and the high costs associated with their treatment give rise to the need\\u000a for the development of wound dressings that protect not only the wounds themselves but that are also able to promote cell\\u000a proliferation and skin regeneration. Moreover, it is also very important that no damage to the new regenerated tissue

C. IgnacioL; L. Barcellos; M. D. Ferreira; S. A. L. Moura; I. A. Soares; R. L. Oréfice

2011-01-01

50

CCMR: Developing a convection asisted wound dressing  

NSDL National Science Digital Library

To develop an improved bandage containing an artificial vascular structure that will effectively treat severe burn wounds. Nutrients injected into the artificial vascular system from outside the body will reach the new cells growing over the wound, and thus allow them to proliferate. With the new cells proliferating, the wound may heal, and a new vascular structure will grow inside the skin to replace the destroyed structure. Since in the bandage, convection is being used to transport nutrients, we call this bandage a convection assisted wound dressing.

Baker, Wesley

2004-08-17

51

Development of nonstick and drug-loaded wound dressing based on the hydrolytic hydrophobic poly(carboxybetaine) ester analogue.  

PubMed

A novel biocompatible polymer is developed for antimicrobial and nonstick coatings of wound dressing. The polymer is formed by copolymerization of carboxybetaine ester analogue methacrylate (CB-ester) and small partial poly(ethylene glycol) methacrylate (PEGMA) for cross-linking by hexamethylene diisocyanate (HDI), which is highly resistant to nonspecific protein adsorption and mammalian cell attachment after a quick hydrolysis. A small hydrophobic drug, aspirin, can be incorporated into the new polymer and slowly released to inhibit microorganism growth while the new polymer shows very low cytotoxicity. Moreover, the wound dressing, the new polymer coated medical gauze, shows good mechanic properties, such as flexibility and strength, for medical application. After all, this new nonfouling polymer offers great potential for an antimicrobial wound dressing and other applications. PMID:24099415

Ji, Fangqin; Lin, Weifeng; Wang, Zhen; Wang, Longgang; Zhang, Juan; Ma, Guanglong; Chen, Shengfu

2013-11-13

52

An in vivo study of hydrogels based on physiologically clotted fibrin-gelatin composites as wound-dressing materials.  

PubMed

In this study we have reported the efficacy of three biomaterials: (a) physiologically clotted fibrin-gelatin composite (PFG), (b) PFG graft copolymerized with 2-hydroxyethyl methacrylate (PFG-HEMA), and (c) PFG graft copolymerized with 2-hydroxypropyl methacrylate (PFG-HPMA) as temporary wound-dressing materials using the rat as an animal model. Full-thickness excision wounds were made on the back of female rats weighing about 150 +/- 10 g. The dressings were applied on the wounds and changed periodically at an interval of 4 days with the respective materials. The wounds treated with PFG-HEMA healed completely on 15th day after wound creation, whereas those treated with PFG and PFG-HPMA resulted in complete healing on the 17th day. The concentrations of collagen, hexosamine, and uronic acid in the granulation tissue were determined. The PFG and its graft copolymers acted as hydrogels, thereby absorbing excess exudates, while still maintaining a moist environment at the wound site. The enhanced wound healing in the experimental animals was reflected in the increased rate of wound contraction. The results of the histological and mechanical studies of the experimental groups revealed that reepithelialization and remodeling of the skin have been achieved by providing a moist environment at the wound site by the biomaterials and thereby hastening the migration of keratinocytes. PMID:15386397

Noorjahan, S E; Sastry, T P

2004-11-15

53

Antibacterial efficacy testing of a bioelectric wound dressing against clinical wound pathogens.  

PubMed

Silver-based wound dressings have been developed for the control of bioburden in wounds. However, the popularity and extensive use of silver-based dressings has been associated with emerging microbial resistances to silver. In this study we examined in vitro antibacterial efficacy of a bioelectric dressing containing silver and zinc against various wound pathogens. Antibiotic-sensitive clinical wound isolates showed a 100% reduction in bacterial growth, except that Enterococcus faecalis isolate was shown to survive with a bacterial log10 reduction rate of less than 10(2) CFU. We also investigated antibacterial efficacy against the extended spectrum ?-lactamase (ESBL) bacteria, multidrug-resistant (MDR) bacteria, and methicillin-resistant Staphylococcus aureus (MRSA). The bioelectric dressing was effective in killing wound pathogens including ESBL, MDR, and MRSA in vitro. Furthermore, based on the primary results against E. faecalis, we carried out extensive studies against several nosocomial Enterococcus species including vancomycin-resistant species. Overall, the vancomycin-sensitive or -resistant Enterococcus species were resistant to this dressing at up to 48 h, except for the vancomycin-resistant Enterococcus raffinosus isolate only showing a 100% bacterial reduction at 48 h, but not at 24 h. The results demonstrated the effective bactericidal activity of a bioelectric dressing against antibiotic-sensitive and MDR strains, but Enterococcus species are bacteriostatic. PMID:24627730

Kim, Hosan; Makin, Inder; Skiba, Jeff; Ho, Amy; Housler, Greggory; Stojadinovic, Alexander; Izadjoo, Mina

2014-01-01

54

Effect of sodium carboxymethylcellulose and fucidic acid on the gel characterization of polyvinylalcohol-based wound dressing.  

PubMed

The purpose of this study was to investigate the effect of sodium carboxymethylcellulose (Na-CMC) and fucidic acid on the gel characterization for the development of sodium fucidate-loaded wound dressing. The cross-linked hydrogel films were prepared with polyvinyl alcohol (PVA) and sodium carboxymethylcellulose (Na-CMC) using the freeze-thawing method. Their gel properties such as gel fraction, swelling, water vapor transmission test, morphology, tensile strength and thermal property were investigated. In vitro protein adsorption test and release were performed. Na-CMC decreased the gel fraction and tensile strength of the hydrogels, but increased the swelling ability, water vapor transmission rate, elasticity and porosity of hydrogels. Thus, the wound dressing developed with PVA and Na-CMC was more swellable, flexible and elastic than that with only PVA because of its cross-linking interaction with PVA. However, the drug had a negative effect on the gel properties of hydrogels but there were no significant differences. In particular, the hydrogel composed of 2.5% PVA, 1.125% Na-CMC and 0.2% drug might give an adequate level of moisture and build up the exudates on the wound area. Thus, this sodium fucidate-loaded hydrogel could be a potential candidate for wound dressing with excellent forming. PMID:20661718

Lim, Soo-Jeong; Lee, Jeong Hoon; Piao, Ming Guan; Lee, Mi-Kyung; Oh, Dong Hoon; Hwang, Du Hyung; Quan, Qi Zhe; Yong, Chul Soon; Choi, Han-Gon

2010-07-01

55

Development of an Ultrafast-Curing Wound Dressing.  

National Technical Information Service (NTIS)

This document describes a second-generation, drug-dispensing wound dressing. The wound dressing, which can be applied by the wounded soldier himself, incorporates thrombin as a coagulant to stop bleeding, and gentamycin sulfate as a wide-spectrum antibiot...

M. Szycher J. L. Rolfe

1985-01-01

56

In vitro evaluation of biocompatibility of different wound dressing materials  

Microsoft Academic Search

The in vitro biocompatibility of newly developed wound dressings consisting of different chitosan salts (chitosan lactate, glutamate and chloride) and a chitosan derivative (methylpyroolidinone chitosan) was compared with three commercially available wound dressings made of collagen, calciumalginate, and gelatin, by evaluation in a fibroblast cell culture system. Three experimental models which reflect relevant stages of wound healing were used, and

P. C. Berscht; B. Nies; A. Liebendörfer; J. Kreuter

1995-01-01

57

BiLayer Wound Dressing System for Combat Casualty Care  

Microsoft Academic Search

SUMMARY Burn injuries remain a significant cause of morbidity and mortality during modern military conflicts and peacekeeping operations. Considering that commercially available dressings are not designed to meet the challenges of treating combat burn wounds, DRDC Toronto has designed a novel, absorbent and medicated bi-layer wound dressing to address key requirements for treating external war wounds. In the present report,

Lucie Martineau; Pang N. Shek

58

Preparation of Polyvinyl Pyrrolidone-Based Hydrogels by Radiation-Induced Crosslinking with Potential Application as Wound Dressing  

Microsoft Academic Search

Polyvinyl pyrrolidone\\/polyethylene glycol hydrogels (PVP\\/PEG) and PVP\\/PEG\\/Starch were prepared by irradiating the mixtures of aqueous solutions of PVP, PEG and starch with electron beam at different doses. Their properties were evaluated to identify their usability in wound dressing applications. The physical properties of the prepared hydrogels, such as gel content, swelling, water content and degree of water evaporation with varying

H. L. Abd El-Mohdy; El-Sayed A. Hegazy

2008-01-01

59

21 CFR 878.4022 - Hydrogel wound dressing and burn dressing.  

Code of Federal Regulations, 2013 CFR

... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4022 Hydrogel wound dressing and burn dressing. (a)...

2013-04-01

60

Removal of adhesive wound dressing and its effects on the stratum corneum of the skin: comparison of eight different adhesive wound dressings.  

PubMed

In recent years, adhesive wound dressings have been increasingly applied postoperatively because of their ease of use as they can be kept in place without having to cut and apply surgical tapes and they can cover a wound securely. However, if a wound dressing strongly adheres to the wound, a large amount of stratum corneum is removed from the newly formed epithelium or healthy periwound skin. Various types of adhesives are used on adhesive wound dressings and the extent of skin damage depends on how much an adhesive sticks to the wound or skin surface. We quantitatively determined and compared the amount of stratum corneum removed by eight different wound dressings including polyurethane foam using acrylic adhesive, silicone-based adhesive dressing, composite hydrocolloid and self-adhesive polyurethane foam in healthy volunteers. The results showed that wound dressings with silicone adhesive and self-adhesive polyurethane foam removed less stratum corneum, whereas composite hydrocolloid and polyurethane foam using acrylic adhesive removed more stratum corneum. PMID:22883604

Matsumura, Hajime; Imai, Ryutaro; Ahmatjan, Niyaz; Ida, Yukiko; Gondo, Masahide; Shibata, Dai; Wanatabe, Katsueki

2014-02-01

61

Comparison of bacteria-retaining ability of absorbent wound dressings.  

PubMed

Fibrous materials in some modern absorbent wound dressings have the ability to sequester and retain bacteria; however, this ability varies according to the nature of the fibres. We studied the bacterial retention capacity of alginate and carboxymethylcellulose dressings, using an infected skin ulcer model on the backs of rats. Wound surfaces were inoculated with either Staphylococcus aureus or Pseudomonas aeruginosa at a concentration of 1.5 x 10(6) colony-forming units per wound. AQUACEL; Hydrofiber;, Kaltostat; or Sorbsan; were applied to the contaminated wounds for 12 h. Each dressing was then divided into two pieces. Total viable bacterial count within the dressing was calculated using one piece, and bacterial count released from the dressing into physiological saline was determined using the other piece, enabling bacterial retention rate to be calculated. Bacterial counts in tissue were also determined. Each dressing was tested on each of 10 wounds contaminated with each bacterium. Statistical analyses were performed using one-way analysis of variance (ANOVA) for replicated measures combined with Duncan's multiple comparison test. AQUACEL; Hydrofiber; dressing was most effective in its ability to retain both Staphylococcus aureus and Pseudomonas aeruginosa (p < 0.05). Bacterial counts in tissue showed no significant change with respect to pathogen or the type of dressing used. It can be concluded that the bacterial retaining ability of AQUACEL; Hydrofiber; dressing was found to be significantly higher than that of alginate dressings in an infected animal wound model. PMID:16722876

Tachi, Masahiro; Hirabayashi, Shinichi; Yonehara, Yoshiyuki; Suzuki, Yasutoshi; Bowler, Philip

2004-09-01

62

Production of hydrogel wound dressings using gamma radiation  

Microsoft Academic Search

Hydrogel wound dressings have been prepared using the gamma rays irradiation technique. The dressings are composed of poly(vinyl pyrrolidone) (PVP), poly(ethylene glycol) (PEG) and agar. The influence of some process parameters on the properties of the dressings has been investigated as: the gel fraction, maximum swelling, swelling kinetics, and mechanical properties. The gel fraction increases with increasing PVP concentration due

Z. Ajji; I. Othman; J. M. Rosiak

2005-01-01

63

Managing wound exudate: role of Versiva XC gelling foam dressing.  

PubMed

Effective management of wound exduate is a considerable challenge for clinicians. Treatment of the patients underlying condition, accurate assessment of the wound and exudate, as well as selection of an appropriate dressing are all important factors for success. A variety of dressings are available to the clinician for the management of exudate, but not all handle fluid in the same way. A gelling foam dressing combining foam with Hydrofiber technology is described and the supporting evidence for its use reviewed. Versiva XC dressing has some good evidence to support its use in the management of wound exudate and performs well in clinical practice. PMID:18773595

Dowsett, Caroline

64

Polymer–xerogel composites for controlled release wound dressings  

Microsoft Academic Search

Many polymers and composites have been used to prepare active wound dressings. These materials have typically exhibited potentially toxic burst release of the drugs within the first few hours followed by a much slower, potentially ineffective drug release rate thereafter. Many of these materials also degraded to produce inflammatory and cytotoxic products. To overcome these limitations, composite active wound dressings

Marius C. Costache; Haibo Qu; Paul Ducheyne; David I. Devore

2010-01-01

65

Absorption of blood by moist wound healing dressings  

Microsoft Academic Search

An understanding of the various properties of the many dressings on the market enables the clinician to select the appropriate dressing product for the patient's wound. A comparative analysis of the ability of moist wound healing (MWH) products to absorb blood has not been previously reported. The aim of this study was to compare the rate and the maximal weight

Patricia Terrill; Geoff Sussman; Michael Bailey

2003-01-01

66

Na-diclofenac ? -cyclodextrin inclusion complex on cotton wound dressing  

Microsoft Academic Search

This paper deals with the production of a wound dressing capable of releasing sodium diclofenac as a drug for curing inflammations. Sodium diclofenac was used as an inclusion compound with ? -cyclodextrin and then attached to the cotton wound dressing by a cross-linking agent, namely, dimethyl dihydroxyl ethylene urea (DMDHEU). Here some analyses were performed such as UV spectrophotometry and

Majid Montazer; Ehsan Bigdeli Mehr

2010-01-01

67

The local treatment and available dressings designed for chronic wounds.  

PubMed

The great diversity of wounds and the broad range of available dressings complicate the selection of proper chronic wound treatment. Choosing the right treatment is the essential step in the healing process. In this review, we focus on chronic nonhealing ulcers, which are a critical problem in clinical practice, and current knowledge about persistent wound care. Here, we present the objectives of local treatment with description of several types of dressings and their ingredients, features, indications, and contraindications. These include hydrocolloid, alginate, hydrogel, and dextranomer dressings; polyurethane foam and membrane dressings; semipermeable polyurethane membrane dressings; and TenderWet (Hartmann, Rock Hill, SC) and flax dressings. There is also a brief section on the use of other alternative wound-healing accelerators, such as platelet-rich plasma and light-emitting diode therapy. PMID:21982060

Skórkowska-Telichowska, Katarzyna; Czemplik, Magdalena; Kulma, Anna; Szopa, Jan

2013-04-01

68

Normal saline wound dressing--is it really normal?  

PubMed

Gauze swabs soaked in normal saline are frequently used as dressing on open wounds. Their exact mechanism of action is not known. This study was designed to assess the hypothesis that normal saline dressings act in part as an osmotic dressing. Ten patients had skin ulcers (n = 10) dressed with normal saline soaked sponges. Acting as controls (n = 10) identical sponges were placed upon intact skin. The sponge fluid osmolarity and electrolyte concentrations were serially assayed to test our hypothesis. In the control group, the osmolarity, sodium and chloride concentrations increased with time as a result of evaporation, altering it from an isotonic to a hypertonic dressing. However, in the ulcer group, the osmolarity, sodium and chloride concentrations in the sponge fluid remained relatively isotonic with time. This result is statistically significant (P< 0.05). We postulate that, as a result of evaporation, the sponge dressing increases its tonicity. This draws fluid from the wound into the dressing so that a dynamic equilibrium occurs and the sponge dressing regains isotonicity. The dressing remains functional provided that the wound fluid is absorbed freely from the wound. This process is terminated when either the dressing completely absorbs the wound fluid or the dressing dries out. The latter often occurs prematurely in a contaminated wound or in a wound where exudate forms a non-permeable barrier which prevents osmosis and allows the remaining water in the dressing to evaporate completely. This correlates with the observation in clinical practice that for maximum efficacy the dressing should be changed regularly. PMID:10657448

Lim, J K; Saliba, L; Smith, M J; McTavish, J; Raine, C; Curtin, P

2000-01-01

69

Development of honey hydrogel dressing for enhanced wound healing  

NASA Astrophysics Data System (ADS)

Radiation at 25 and 50 kGy showed no effect on the acidic pH of the local honey, Gelam, and its antimicrobial property against Staphylococcus aureus but significantly reduced the viscosity. Honey stored up to 2 years at room temperature retained all the properties studied. Radiation sterilized Gelam honey significantly stimulated the rate of burn wound healing in Sprague-Dawley rats as demonstrated by the increased rate of wound contraction and gross appearance. Gelam honey attenuates wound inflammation; and re-epithelialization was well advanced compared to the treatment using silver sulphadiazine (SSD) cream. To enhance further the use of honey in wound treatment and for easy handling, Gelam honey was incorporated into our hydrogel dressing formulation, which was then cross-linked and sterilized using electron beam at 25 kGy. Hydrogel with 6% of honey was selected based on the physical appearance.

Yusof, Norimah; Ainul Hafiza, A. H.; Zohdi, Rozaini M.; Bakar, Md Zuki A.

2007-11-01

70

Sequential antibiotic and growth factor releasing chitosan-PAAm semi-IPN hydrogel as a novel wound dressing  

Microsoft Academic Search

The aim of this study is to prepare a novel wound dressing material which provides burst release of an antibiotic in combination with sustained release of growth factor delivery. This might be beneficial for the prevention of infections and to stimulate wound healing. As a wound dressing material, the semi-interpenetrating network (semi-IPN) hydrogel based on polyacrylamide (PAAm) and chitosan (CS)

Mehlika Pulat; An?l Sera Kahraman; Nur Tan; Menem?e Gümü?derelio?lu

2012-01-01

71

Hydrocellular Foam Dressing Promotes Wound Healing along with Increases in Hyaluronan Synthase 3 and PPAR? Gene Expression in Epidermis  

PubMed Central

Background Hydrocellular foam dressing, modern wound dressing, induces moist wound environment and promotes wound healing: however, the regulatory mechanisms responsible for these effects are poorly understood. This study was aimed to reveal the effect of hydrocellular foam dressing on hyaluronan, which has been shown to have positive effects on wound healing, and examined its regulatory mechanisms in rat skin. Methodology/Principal Findings We created two full-thickness wounds on the dorsolateral skin of rats. Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured. We also investigated the mechanism by which the hydrocellular foam dressing regulates hyaluronan production by measuring the gene expression of hyaluronan synthase 3 (Has3), peroxisome proliferator-activated receptor ? (PPAR?), and CD44. Hydrocellular foam dressing promoted wound healing and upregulated hyaluronan synthesis, along with an increase in the mRNA levels of Has3, which plays a primary role in hyaluronan synthesis in epidermis. In addition, hydrocellular foam dressing enhanced the mRNA levels of PPAR?, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44. Conclusions/Significance These findings suggests that hydrocellular foam dressing may be beneficial for wound healing along with increases in hyaluronan synthase 3 and PPAR? gene expression in epidermis. We believe that the present study would contribute to the elucidation of the mechanisms underlying the effects of hydrocellular foam dressing-induced moist environment on wound healing and practice evidence-based wound care.

Yamane, Takumi; Nakagami, Gojiro; Yoshino, Sawako; Muramatsu, Aimi; Matsui, Sho; Oishi, Yuichi; Kanazawa, Toshiki; Minematsu, Takeo; Sanada, Hiromi

2013-01-01

72

WOUND DRESSINGS ON RED MAPLE AND AMERICAN ELM: EFFECTIVENESS AFTER FIVE YEARS  

Microsoft Academic Search

1 Abstract. Closure and internal compartmentalization of wounds on red maple and American elm were not stimulated by dressings of an asphalt-based material, orange shellac, or polyurethane varnish. After 5 years, decay fungi had infected many treated and control wounds. Some trees closed woun- ds rapidly; others closed wounds slowly regardless of the treatments.

Alex L. Shigo; Charles L. Wilson

73

Redressing a Wound with a Sterile Dressing (VHS 3/4 inch) (Video).  

National Technical Information Service (NTIS)

Shows the procedures for replacing a wound dressing, including washing hands, removing old dressing, evaluating wound drainage, application of a sterile dressing using aseptic, and disposing of contaminated materials.

1983-01-01

74

Redressing a Wound with a Sterile Dressing (VHS 1/2 inch) (Video).  

National Technical Information Service (NTIS)

The program shows the procedures for replacing a wound dressing, including washing hands, removing old dressing, evaluating wound drainage, application of a sterile dressing using aseptic, and disposing of contaminated materials.

1983-01-01

75

Current trends in the development of wound dressings, biomaterials and devices.  

PubMed

Wound management covers all aspects of patient care from initial injury, treatment of infection, fluid loss, tissue regeneration, wound closure to final scar formation and remodeling. There are many wound-care products available including simple protective layers, hydrogels, metal ion-impregnated dressings and artificial skin substitutes, which facilitate surface closure. This review examines recent developments in wound dressings, biomaterials and devices. Particular attention is focused on the design and manufacture of hydrogel-based dressings, their polymeric constituents and chemical modification. Finally, topical negative pressure and hyperbaric oxygen therapy are considered. Current wound-management strategies can be expensive, time consuming and labor intensive. Progress in the multidisciplinary arena of wound care will address these issues and be of immense benefit to patients, by improving both clinical outcomes and their quality of life. PMID:24237061

Martin, Claire; Low, Wan Li; Amin, Mohd Cairul Iqbal Mohd; Radecka, Iza; Raj, Prem; Kenward, Ken

2013-05-01

76

Modified wound dressing with phyto-nanostructured coating to prevent staphylococcal and pseudomonal biofilm development.  

PubMed

This paper reports a newly fabricated nanophyto-modified wound dressing with microbicidal and anti-adherence properties. Nanofluid-based magnetite doped with eugenol or limonene was used to fabricate modified wound dressings. Nanostructure coated materials were characterized by TEM, XRD, and FT-IR. For the quantitative measurement of biofilm-embedded microbial cells, a culture-based method for viable cell count was used. The optimized textile dressing samples proved to be more resistant to staphylococcal and pseudomonal colonization and biofilm formation compared to the uncoated controls. The functionalized surfaces for wound dressing seems to be a very useful tool for the prevention of wound microbial contamination on viable tissues. PMID:23272823

Anghel, Ion; Holban, Alina Maria; Grumezescu, Alexandru Mihai; Andronescu, Ecaterina; Ficai, Anton; Anghel, Alina Georgiana; Maganu, Maria; Laz R, Veronica; Chifiriuc, Mariana Carmen

2012-01-01

77

Compatibility of a protein topical gel with wound dressings.  

PubMed

The compatibility between several dressing materials and a recombinant human vascular endothelial growth factor (rhVEGF) topical methylcellulose gel formulation was investigated. The dressings being studied were Adaptic, Non-stick Dressing, Conformant 2, Opsite and Tegapore. The criteria to select a compatible dressing include protein stability, absence of leachables from the dressing, and ability to retain gel on wound. An LC-MS method with sample treatment using cellulase was developed to determine protein oxidation in gel formulations. Results showed that rhVEGF was significantly oxidized by Adaptic dressing in 24 h. Protein oxidation was likely due to the peroxides, as determined by FOX assay, released into the protein solution from the dressing. Furthermore, Adaptic dressing caused protein adsorption loss, formation of high MW protein adducts, and released leachables as determined by RP-HPLC, LC-MS, and SEC. No protein oxidation or loss was observed after exposure to the other four alternative dressings. However, unknown leachables were detected in the presence of Opsite and Non-stick Dressing. The pore sizes of the Conformant 2 and Non-stick dressings were too large to hold the topical gel within the wound area, making them unsuitable for patient use. No rhVEGF bioactivity loss was observed in the presence of Tegapore. In conclusion, Tegapore was considered suitable for the rhVEGF topical gel. PMID:18623201

Ji, Junyan A; Borisov, Oleg; Ingham, Erika; Ling, Victor; Wang, Y John

2009-02-01

78

Development and in vitro evaluation of chitosan-Eudragit RS 30D composite wound dressings  

Microsoft Academic Search

The purpose of this research was to design and evaluate chitosan-based films intended for wound dressing application. Required\\u000a properties for successful wound dressing, such as liquid uptake, vapor and oxygen penetration, bioadhesiveness, and film elasticity,\\u000a were examined. Water uptake and vapor penetration of the films were determined gravimetrically, while oxygen penetration was\\u000a determined by Winkler’s method. The bioadhesive properties were

Sakchai Wittaya-areekul; Chureerat Prahsarn; Srisagul Sungthongjeen

2006-01-01

79

21 CFR 878.4018 - Hydrophilic wound dressing.  

Code of Federal Regulations, 2013 CFR

... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4018 Hydrophilic wound dressing. (a) Identification. A...

2013-04-01

80

21 CFR 878.4020 - Occlusive wound dressing.  

Code of Federal Regulations, 2013 CFR

... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4020 Occlusive wound dressing. (a) Identification. An occlusive...

2013-04-01

81

A Dressing Solution for Burn Wounds: Antibacterial and Low-Adherent Wound Dressings  

NASA Astrophysics Data System (ADS)

Considering the infection and second trauma caused by dressing changes, development of antibacterial and low-adherent wound dressings is urgently needed. Silver ion is a widely used antimicrobial agent, but its cytotoxicity remains a problem. In this study, low-adherent PAM (polyacrylamide) hydrogel incorporated with less toxic AgNP (silver nanoparticle), was immobilized onto PET (poly(ethylene terephthalate)) substrates by an IPN (interpenetrating polymer network) method. The modified PET is effectively antibacterial and the surface is significantly less adherent than untreated PET. However, silver-resistant bacteria become a potential problem. Thus, ionic 5,5-dimethylhydantoin (DMH) analogues containing either a quaternary ammonium moiety or a phosphonate functional group were designed and synthesized. The DMH analogues were converted to antibacterial N-chloramine counterparts through chlorination to serve as potential alternatives to AgNP. The N-chloramine with a structural cation exhibited distinctly enhanced antibacterial functions both in solution and after immobilization on fabrics.

Pu, Tianyun

82

Honey-based PET or PET/chitosan fibrous wound dressings: effect of honey on electrospinning process.  

PubMed

In this study, fibrous mats were fabricated via electrospinning from solutions of polyethylene terephthalate (PET), PET/chitosan, and PET/honey at different concentrations. The effect of honey and chitosan on electrospinning process was investigated and compared. Fibers containing chitosan had a beaded or ribbon-like/branched morphology, but this morphology improved in the presence of honey. The diameter of electrospun fibers decreased with an increased ratio of honey in PET solution. In addition, fiber deposition area in the collector increased by increasing the honey content. PET/chitosan and PET/honey fibrous mats reached an equilibrium water content in 15?min and their water uptake capacities, which are important for exudating wounds, were found in the range of 280-430% on dry basis. Cytotoxicity evaluation demonstrated that fibers exhibited no cytotoxic activity. This study discloses that PET fibrous mats especially electrospun in the presence of honey could be proposed as potential wound dressing materials owing to their improved processing abilities besides their suitable structural properties. PMID:24842308

Arslan, Aysu; Sim?ek, Murat; Aldemir, Sevcan Dalk?rano?lu; Kazaro?lu, Nur Merve; Gümü?derelio?lu, Menem?e

2014-07-01

83

Slow release of wound healing drug from hydrogel wound dressing prepared by radiation crosslinking method  

NASA Astrophysics Data System (ADS)

The hydrogel wound dressing was prepared by radiation crosslinking. It was used of on patients in the Navy 411 Hospital and some other hospitals. From sixty case studies of the clinical effects, the results showed that: 1. drug releasing slowly releives the pain effectively for prolonged period of application; 2. The dressing can reduce the oozing liquid from the wound and make the wound heal faster; 3. The number of the dressing change is greatly reduced. All the data indicates that the dressing is superior to the conventional kinds.

Weibin, Wu; Peizhi, Sung; Xingguo, Wang; Jinghui, Li; Jie, Chen; Yueqi, Yang; Yihao, Shen; Zueteh, Ma

1993-10-01

84

Slow release of wound healing drug from hydrogel wound dressing prepared by radiation crosslinking method  

Microsoft Academic Search

The hydrogel wound dressing was prepared by radiation crosslinking. It was used of on patients in the Navy 411 Hospital and some other hospitals. From sixty case studies of the clinical effects, the results showed that: 1. drug releasing slowly releives the pain effectively for prolonged period of application; 2. The dressing can reduce the oozing liquid from the wound

Wu Weibin; Sung Peizhi; Wang Xingguo; Li Jinghui; Chen Jie; Yang Yueqi; Shen Yihao; Ma Zueteh

1993-01-01

85

Wound healing dressings and drug delivery systems: a review.  

PubMed

The variety of wound types has resulted in a wide range of wound dressings with new products frequently introduced to target different aspects of the wound healing process. The ideal dressing should achieve rapid healing at reasonable cost with minimal inconvenience to the patient. This article offers a review of the common wound management dressings and emerging technologies for achieving improved wound healing. It also reviews many of the dressings and novel polymers used for the delivery of drugs to acute, chronic and other types of wound. These include hydrocolloids, alginates, hydrogels, polyurethane, collagen, chitosan, pectin and hyaluronic acid. There is also a brief section on the use of biological polymers as tissue engineered scaffolds and skin grafts. Pharmacological agents such as antibiotics, vitamins, minerals, growth factors and other wound healing accelerators that take active part in the healing process are discussed. Direct delivery of these agents to the wound site is desirable, particularly when systemic delivery could cause organ damage due to toxicological concerns associated with the preferred agents. This review concerns the requirement for formulations with improved properties for effective and accurate delivery of the required therapeutic agents. General formulation approaches towards achieving optimum physical properties and controlled delivery characteristics for an active wound healing dosage form are also considered briefly. PMID:17963217

Boateng, Joshua S; Matthews, Kerr H; Stevens, Howard N E; Eccleston, Gillian M

2008-08-01

86

Wound dressing adherence: a clinical comparative study.  

PubMed Central

This prospective study was undertaken to compare the adherence of dressings currently used as non-adherent dressings. Four different dressings were studied on a total of 40 patients. An overall adherence of 50% was encountered, Silicon Polymer Foam being the most successful under the trial conditions.

Malone, W D

1987-01-01

87

Effects of silver-based wound dressings on the bacterial flora in chronic leg ulcers and its susceptibility in vitro to silver.  

PubMed

Silver-based dressings have been used extensively in wound management in recent years, but data on their antimicrobial activity in the clinical setting are limited. In order to explore their effects on chronic leg ulcer flora, 14 ulcers were cultured after at least 3 weeks treatment with Aquacel Ag(®) or Acticoat(®). Phenotypic and genetic silver resistance were investigated in a total of 56 isolates. Silver-based dressings had a limited effect on primary wound pathogens, which were present in 79% of the cultures before, and 71% after, treatment. One silver-resistant Enterobacter cloacae strain was identified (silver nitrate minimal inhibitory concentration (MIC)?>?512 mg/l, positive for silE, silS and silP). Further studies in vitro showed that inducible silver-resistance was more frequent in Enterobacteriaceae with cephalosporin-resistance and that silver nitrate had mainly a bacteriostatic effect on Staphylococcus aureus. Monitoring of silver resistance should be considered in areas where silver is used extensively. PMID:22215013

Sütterlin, Susanne; Tano, Eva; Bergsten, Agneta; Tallberg, Anna-Britta; Melhus, Asa

2012-01-01

88

Production of hydrogel wound dressings using gamma radiation  

NASA Astrophysics Data System (ADS)

Hydrogel wound dressings have been prepared using the gamma rays irradiation technique. The dressings are composed of poly(vinyl pyrrolidone) (PVP), poly(ethylene glycol) (PEG) and agar. The influence of some process parameters on the properties of the dressings has been investigated as: the gel fraction, maximum swelling, swelling kinetics, and mechanical properties. The gel fraction increases with increasing PVP concentration due to increased crosslink density, and decreases with increasing the PEG concentration. PEG seems to act not only as plasticizer but also to modify the gel properties as gelation% and maximum swelling. The prepared hydrogels dressings could be considered as a good barrier against microbes.

Ajji, Z.; Othman, I.; Rosiak, J. M.

2005-04-01

89

Histological evaluation of wound healing performance of electrospun poly(vinyl alcohol)/sodium alginate as wound dressing in vivo.  

PubMed

Poly(vinyl alcohol)/sodium alginate nanofibrous mats were produced by electrospinning method at optimum process parameters. Evaluation of alginate-based electrospun nanofibrous mats as a wound dressing material and their comparison to commercially available wound dressings produced with conventional methods were carried out in vivo. Tissue specimens were examined histopathologically on 4th, 6th, 15th, 21st postoperative days. In contrast to other dressings it was observed that nanofibrous mat could survive on the wound crust in early stages of healing. In terms of epithelization, epidermis characteristics, vascularization and formation of hair follicles, nanofibrous mat showed the best healing performance. This could be explained with presence of nanofibrous mat acting as an artificial skin on the wound region until new tissue regenerated. PMID:24642979

Co?kun, Gökçe; Karaca, Esra; Ozyurtlu, Mustafa; Ozbek, Serhat; Yermezler, Aysun; Cavu?o?lu, Ilkin

2014-01-01

90

Clinical experience with the negative pressure wound dressing  

Microsoft Academic Search

Between 1997 and 1999, more than 300 patients have been treated using negative pressure wound dressings. The technique has been used successfully to prepare various acute, chronic or infected wounds to accept a skin graft or flap, and to promote graft take at difficult donor sites. The advantages include rapid healing by secondary intention, reduced time to skin grafting, an

C. Avery; J. Pereira; A. Moody; I. Whitworth

2000-01-01

91

Biological properties of the chitosan-gelatin sponge wound dressing  

Microsoft Academic Search

In order to testify the safe reliability, antibacterial property and wound healing ability in clinic application of the chitosan-gelatin sponge wound dressing (CGSWD), a lot of experiments were executed : (1) A series of toxicological evaluations of the CGSWD were performed. (2) The antibacterial properties of the CGSWD obtained by different dryness ways were investigated, and then compared the antibacterial

Chun-Mei Deng; Lan-Zhen He; Ming Zhao; Dan Yang; Yi Liu

2007-01-01

92

Comparative Study of Antibacterial Effects and Bacterial Retentivity of Wound Dressings  

PubMed Central

Objectives: We are often confused on selecting a suitable wound dressing for the treatment of infected wounds from huge number of available wound dressings. Then, to help clinicians easily select a wound dressing, we compared the antibacterial effects and bacterial retentivity (ie, potency of keeping absorbed bacteria inside wound dressings and preventing them from leaking out) of wound dressings. Methods: Five wound dressings with antibacterial constituents were compared to research antibacterial effects against nonpathogenic Escherichia coli using an in vitro model. The 5 other wound dressings with no antibacterial constituent were compared to research bacterial retentivity. The relative amount of E coli was determined using cell proliferation reagent WST-1 (11644807001, Roche Applied Science, United States) with time. Results: The results have shown that the antibacterial effects and bacterial retentivity differed among various wound dressings. Silver ions quickly exerted a very strong antibacterial effect, and hydrofibers had a high potency of bacterial retentivity by gelling the absorbed bacteria in wound dressings. Conclusions: The present study indicated the differences of antibacterial strength, time of onset and duration of the antibacterial effect, and bacterial retentivity between each wound dressing. Clinicians should use appropriate wound dressings according the wound condition in consideration of the different characteristics of wound dressings. The present results are helpful for clinicians to select appropriate wound dressing.

Fujiwara, Toshihiro; Hosokawa, Ko; Kubo, Tateki

2013-01-01

93

Evaluation of chitosan nano dressing for wound healing: characterization, in vitro and in vivo studies.  

PubMed

In our present investigation, a ternary nano dressing consists of titanium dioxide nano particle loaded chitosan-pectin was prepared to evaluate biocompatibility, antimicrobial and in vivo wound healing properties. The photoactive property of TiO? based materials makes it important candidate for numerous medical applications. Chitosan can be easily processed into membranes, gels, nanofibers, beads, nanoparticles, scaffolds, and sponge forms that can be used in wound healing applications. Pectin acts as a natural prophylactic substance against poisoning with toxic cations and its styptic and curing effects are well documented in healing ointments. The characterizations of prepared nano dressing were made by FTIR, TGA, DSC, SEM and TEM. The physicochemical parameters of nano dressing were evaluated by various techniques, namely, the Whole blood clotting test, haemolysis ratio measurement, cytotoxicity test using NIH3T3 and L929 fibroblast cells. The in vivo open excision-type wound healing efficiency of prepared nano dressing and its comparison with conventional gauze were evaluated by measuring wound contraction and histological examinations in adult male albino rats. The synergistic effects of nano dressing such as good antibacterial ability, high swelling properties, high water vapour transmission rate (WVTR), excellent hydrophilic nature, biocompatibility, wound appearance, wound closure rate and histological study through in vivo test makes it a suitable candidate for wound healing applications. PMID:23518244

Archana, D; Dutta, Joydeep; Dutta, P K

2013-06-01

94

Evaluation of banana leaf dressing for partial thickness burn wounds.  

PubMed

An important factor in the healing of superficial and moderate partial thickness burn is early and effective coverage with a dressing that protects the wound from trauma and dessication and is non-adherent. In our country cost is also a very important factor. Disturbed by the pain and anxiety experienced by the patients during and after dressing changes, search for a new dressing material was begun for partial thickness burn wounds and the banana leaf dressing (BLD) was subsequently developed and optimised by June 1996. An open controlled study was carried out to compare banana leaf dressing and boiled potato peel bandage (BPPB), the dressing being used in our Burn Unit since 1994. Thirty patients all less than 40 years of age, with burn size less than 50% TBSA, involving comparable body areas with partial thickness burn, were included in the study. BLD along with a topical agent was applied over the right sided extremity while BPPB was applied with the same topical agent on the left sided extremity. Dressing was changed every day. The pain during dressing change, feeling of comfort and ease of handling dressing was assessed by awarding scores by the patients and care givers for each type of dressing. Analysis of the scores revealed that both BLD and BPPB caused easily tolerable, minimal pain during dressing change in majority of the patients. The days taken for epithelialisation, eschar formation and the need for skin grafting over deep partial thickness burns, did not reveal any significant difference between the areas treated by BLD and those by BPPB. So both the dressings were observed to have equal efficacy in protecting the wounds and aiding healing. Thus, the efficacy of BLD and BPPB was parallel in all respects. But BLD is 11 times cheaper than BPPB. Banana plants can be easily grown, the leaves are easily available throughout the year. The leaves of banana are large thus offering larger surface area and the surface is non-adherent, waxy and cool. The dressing can be prepared very easily with little training. It is also the cheapest dressing available today. We strongly recommend the use of banana leaf dressing for all partial thickness burn wounds in our environment. PMID:12880731

Gore, Madhuri A; Akolekar, Deepika

2003-08-01

95

A comparison of three wound dressings in patients undergoing heart surgery.  

PubMed

Two hundred fifty patients undergoing heart surgery were randomized in a prospective comparative study of a semiocclusive hydroactive wound dressing, an occlusive hydrocolloid dressing, and a conventional absorbent dressing. The wounds were evaluated during the 4 weeks after surgery. Color photographs were used for a blind evaluation of wound healing. The conventional absorbent dressing was more effective in wound healing, compared with the hydroactive dressing. Further, there were fewer skin changes and less redness in the wounds with the conventional dressing than with the hydroactive dressing; the differences were not significant with the hydrocolloid dressing. The conventional dressing was less painful to remove than the hydroactive and hydrocolloid dressings. More frequent dressing changes, however, were needed when using the conventional dressing. Despite this, it was the least expensive alternative. PMID:7567488

Wikblad, K; Anderson, B

1995-01-01

96

Synthesis and characterization of semi-interpenetrating polymer networks based on polyurethane and N-isopropylacrylamide for wound dressing.  

PubMed

Thermosensitive semi-interpenetrating polymer networks (semi-IPNs) composed of crosslinked poly(N-isopropylacrylamide) (PNiPAAm) and linear segmented polyurethane urea (SPUU) were synthesized via thermal initiated free radical polymerization. Synthesized semi-IPNs of various compositions were characterized by Fourier transform infrared spectroscopy, water equilibrium swelling at different temperatures, drug lading, drug release, cell adhesion, and detachment. The semi-IPN films of all the compositions were transparent in dry state and negative thermosensitivity in their swelling ratio, that is, lower swelling levels with increasing temperature. The drug release study revealed that the rate of drug release is fast in case of pure SPUU compared to PNiPAAm and semi-IPN film. Drug release depended mainly on solubility of the drugs and physical networks between SPUU and PNiPAAm. Finally NIH3T3 cells were seeded on the semi-IPN films and found that cells were securely attached and proliferated to confluence. Upon cooling, cells were detached from the semi-IPN films. Therefore, the semi-IPN films may be good candidate materials for wound dressing applications. PMID:18780359

Reddy, Thatiparti Thimma; Kano, Arihiro; Maruyama, Atsushi; Hadano, Michiko; Takahara, Atsushi

2009-01-01

97

Honey Dressing Versus Silver Sulfadiazene Dressing for Wound Healing in Burn Patients: A Retrospective Study  

PubMed Central

Objective: The aim was to evaluate the effect of honey dressing and silver sulfadiazene (SSD) dressing on wound healing in burn patients. Materials and Methods: We retrospectively reviewed the records of 108 patients (14–68 years of age), with first and second degree burns of less than 50% of the total body surface area admitted to our institution, over a period of 5 years (2004–2008). Fifty-one patients were treated with honey dressings and 57 with SSD. Time elapsed since burn, site, percentage, degree and depth of burns, results of culture sensitivity at various time intervals, duration of healing, formation of post-treatment hypertrophic scar, and/or contracture were recorded and analyzed. Results: The average duration of healing was 18.16 and 32.68 days for the honey and SSD group, respectively. Wounds of all patients reporting within 1 h of burns became sterile with the honey dressing in less than 7 days while there was none with SSD. All wounds treated with honey became sterile within 21 days while for SSD-treated wounds, this figure was 36.5%. A complete outcome was seen in 81% of all patients in the “honey group” while in only 37% patients in the “SSD group.” Conclusion: Honey dressings make the wounds sterile in less time, enhance healing, and have a better outcome in terms of hypertropic scars and postburn contractures, as compared to SSD dressings.

Gupta, Shilpi Singh; Singh, Onkar; Bhagel, Praveen Singh; Moses, Sonia; Shukla, Sumit; Mathur, Raj Kumar

2011-01-01

98

Antibiofilm Efficacy of DispersinB® Wound Spray Used in Combination with a Silver Wound Dressing  

PubMed Central

Chronic wounds including diabetic foot ulcers, pressure ulcers, and venous leg ulcers are a worldwide health problem. As the traditional methods of treatment have proven ineffective against chronic wounds involving biofilms, there is an unmet clinical need for developing products with an antibiofilm component that inhibits and/or disrupts biofilms and thus make the biofilm-embedded bacteria more susceptible to antimicrobial therapy. We developed a DispersinB® antibiofilm enzyme-based wound spray for treating chronic wounds in conjunction with an antimicrobial. Under in vitro conditions, the DispersinB® and Acticoat™ combination performed significantly better (P < 0.05) than Acticoat™ alone, indicating the synergy between the two compounds because of DispersinB® enhancing the antimicrobial activity of Acticoat™. Furthermore, DispersinB® wound spray enhanced the antimicrobial activity of Acticoat™ in a chronic wound mouse model of methicillin-resistant Staphylococcus aureus (MRSA) infection. Thus, this novel combination of DispersinB® and Acticoat™, an antimicrobial dressing, prompts clinical evaluation for potential applications in biofilm-based chronic wound management.

Gawande, Purushottam V; Clinton, Allie P; LoVetri, Karen; Yakandawala, Nandadeva; Rumbaugh, Kendra P; Madhyastha, Srinivasa

2014-01-01

99

Antibiofilm Efficacy of DispersinB(®) Wound Spray Used in Combination with a Silver Wound Dressing.  

PubMed

Chronic wounds including diabetic foot ulcers, pressure ulcers, and venous leg ulcers are a worldwide health problem. As the traditional methods of treatment have proven ineffective against chronic wounds involving biofilms, there is an unmet clinical need for developing products with an antibiofilm component that inhibits and/or disrupts biofilms and thus make the biofilm-embedded bacteria more susceptible to antimicrobial therapy. We developed a DispersinB® antibiofilm enzyme-based wound spray for treating chronic wounds in conjunction with an antimicrobial. Under in vitro conditions, the DispersinB® and Acticoat™ combination performed significantly better (P < 0.05) than Acticoat™ alone, indicating the synergy between the two compounds because of DispersinB® enhancing the antimicrobial activity of Acticoat™. Furthermore, DispersinB® wound spray enhanced the antimicrobial activity of Acticoat™ in a chronic wound mouse model of methicillin-resistant Staphylococcus aureus (MRSA) infection. Thus, this novel combination of DispersinB® and Acticoat™, an antimicrobial dressing, prompts clinical evaluation for potential applications in biofilm-based chronic wound management. PMID:24826078

Gawande, Purushottam V; Clinton, Allie P; LoVetri, Karen; Yakandawala, Nandadeva; Rumbaugh, Kendra P; Madhyastha, Srinivasa

2014-01-01

100

Development of an Ultra-Fast-Curing Wound Dressing. Annual Report no. 2 October 1, 1985 - June 30, 1986.  

National Technical Information Service (NTIS)

The authors are developing a drug-dispensing field wound dressing. The wound dressing, which can be easily applied by an untrained person, contains a coagulant stop bleeding, and an antibiotic to prevent bacterial infection. The medicated wound dressing i...

M. Szycher J. L. Rolfe

1987-01-01

101

Endotoxin contamination in wound dressings made of natural biomaterials.  

PubMed

Contamination by endotoxin of nine kinds of wound dressings made of natural biomaterials (calcium alginate, collagen, chitin, and poly-L-leucine) was examined with the use of water extracts. By applying the Limulus amoebocyte lysate (LAL) test, high concentrations of endotoxin were detected in extracts from three kinds of products made of calcium alginate. These extracts evoked fever in rabbits and induced the release of a proinflammatory (pyrogenic) cytokine, interleukin-6 (IL-6), from human monocytic cells (MM6-CA8). The effects disappeared when the extracts were treated with endotoxin-removing gel column chromatography or with an endotoxin antagonist, B464, confirming that the contaminating pyrogen was endotoxin. A noteworthy finding was that one of the endotoxin-containing extracts showed very weak IL-6-inducibility in human monocytic cells in contrast to its high pyrogenicity to rabbits. The discrepancy could be explained based on differences between humans and rabbits in sensitivity to the endotoxin, because the extract showed higher proinflammatory-cytokine (TNF-alpha)-inducibility in rabbit whole-blood cells (WBCs) than human WBCs. The results suggest that the LAL test is a useful method of detecting endotoxin contamination in wound dressings and the MM6-CA8 assay is a good supplement to the LAL test for evaluating pyrogenicity in humans accurately. PMID:12808594

Nakagawa, Y; Murai, T; Hasegawa, Chie; Hirata, M; Tsuchiya, T; Yagami, T; Haishima, Y

2003-07-15

102

A Prospective Randomized Evaluation of Negative-pressure Wound Dressings for Diabetic Foot Wounds  

Microsoft Academic Search

Optimal treatment for large diabetic foot wounds is ill defined. The purpose of this study was to compare the rate of wound healing with the Vacuum Assisted Closure device™ (VAC) to conventional moist dressings in the treatment of large diabetic foot wounds. Diabetics with significant soft tissue defects of the foot were considered for enrollment. Patients were randomized to receive

Mark T. Eginton; Kellie R. Brown; Gary R. Seabrook; Jonathan B. Towne; Robert A. Cambria

2003-01-01

103

A review of the applications of the hydrofiber dressing with silver (Aquacel Ag®) in wound care  

PubMed Central

Aquacel Ag® (ConvaTec, Princeton, NJ, USA) is a new hydrofiber wound dressing consisting of soft non-woven sodium carboxymethylcellulose fibers integrated with ionic silver. It is a moisture-retention dressing, which forms a gel on contact with wound fluid and has antimicrobial properties of ionic silver. We present a current literature review on Aquacel Ag®, of both in vitro and in vivo efficacy and clinical applications. In vitro and in vivo studies have demonstrated the wide antimicrobial properties of Aquacel Ag®, and additionally demonstrated the cytotoxicity of ionic silver to keratinocytes and fibroblasts that cause delay in wound re-epithelialization. Clinical studies confirmed that Aquacel Ag® is an effective and safe dressing for a variety of wound types, both acute and chronic. Incorporation of ionic silver into the hydrofibers does not cause undue alteration in the performance properties of the base dressing, which continues to provide favorable wound moisture and exudate management. The addition of ionic silver reduces local pain and dressing changes, and provides significant broad-spectrum antimicrobial properties, with no delay in wound healing.

Barnea, Yoav; Weiss, Jerry; Gur, Eyal

2010-01-01

104

Situating wound management: technoscience, dressings and 'other' skins.  

PubMed

This paper addresses the notion of wound care as a technology of skin and other skins imbued with the combined power of technology and science. It presents the discourses of wound care evident in the accounts of patients and nurses concerning this care, and discussions about wounds in wound care interest groups, journals, and advertising material about wound care products. The discussion focuses on wounds and wound dressings as effects immanent in the power relations of discourses of wound care. These effects colour and influence nurses' responses to wounds and wound care products. Moreover, the discourses that portray these practices are evidence of the complex articulation between technoscience and gender. Nurses and patients are fascinated by wound technoscience and lured towards it by its potential for mastery and control over wounds. Such seductions are evident in the texts of nurses, patients, and pharmaceutical advertisements for wound care products. Finally, the ways that these representations are used to talk about and market wound care products are shown as exemplifying the finer points of wound management as a nursing technoscience. PMID:10795270

Rudge, T

1999-09-01

105

Nanocrystalline silver dressings in wound management: a review  

PubMed Central

This paper describes the properties of nanocrystalline silver products (Acticoat™) and their applications and examines available evidence supporting their use in wound management. Acticoat utilizes nanotechnology to release nanocrystalline silver crystals. Acticoat releases 30 times less silver cations than silversulfadiazine cream or 0.5% silver nitrate solution but more of the silver released (by Acticoat). Silver-impregnated slow-release dressings release minute concentrations of silver which are quickly bound up by the chloride in the wound exudate. While extrapolations from in vitro and animal studies are cautious, evidence from these studies suggests Acticoat is: effective against most common strains of wound pathogens; can be used as a protective covering over skin grafts; has a broader antibiotic spectrum activity; and is toxic to keratinocytes and fibroblasts. Animal studies suggest a role for nanocrystalline silver in altering wound inflammatory events and facilitation of the early phase of wound healing. Quality human clinical trials into nanocrystalline silver are few. However, evidence suggests using Acticoat in wound management is cost effective, reduces wound infection, decreases the frequency of dressing changes and pain levels, decreases matrix metalloproteinase activity, wound exudate and bioburden levels, and promotes wound healing in chronic wounds. Although there is no in vivo evidence to suggest nanocrystalline silver is toxic to human keratinocytes and fibroblasts, there is in vitro evidence to suggest so; thus these dressings should be used cautiously over epithelializing and proliferating wounds. Future clinical research, preferably randomized controlled trials into nanocrystalline silver technology, may provide clinicians a better understanding of its applications in wound management.

Fong, Joy; Wood, Fiona

2006-01-01

106

Management of partial skin thickness burn wounds with Inadine dressings.  

PubMed

In a prospective randomized trial, 213 consecutive patients with less than 10 per cent BSA partial thickness burns were treated as outpatients with either Bactigras (n = 102) (tulle gras dressing with 0.5 per cent Chlorhexidine Acetate B.P.) or Inadine (n = 111) (rayon dressing with 10 per cent povidone iodine ointment). Inadine caused less bleeding on dressing removal but not significantly less. Inadine treated patients required less analgesia, a reduced treatment time, a smaller number of hospital visits and less time off work/normal activities (P = 0.01). Inadine should be more widely used in the management of partial thickness burn wounds. PMID:2696483

Han, K H; Maitra, A K

1989-12-01

107

Inflammatory inert poly(ethylene glycol)--protein wound dressing improves healing responses in partial- and full-thickness wounds.  

PubMed

In this study, a novel soft hydrogel system based on the poly(ethylene glycol)-protein conjugates was evaluated as an occlusive wound dressing material. The hydrogel material, referred by the name of BioAquacare, contains up to 96% of the liquid and is formulated with phosphate-buffered saline and safe preservative to control bacterial load in the open wounds. Performance of the BioAquacare as a wound dressing material was assessed in partial- and full-thickness wounds in pigs. Wound analysis comprised macroscopic determination of the wound size, histological examination of the healing tissues and biochemical characterisation of wound exudates. The wounds treated with BioAquacare healed without any signs of inflammation, skin irritation, oedema or erythema. Cellular composition of the reepithelialised wounds was very similar to that of the normal skin, with a well-developed stratum corneum and epithelial layer. It was observed that BioAquacare plays the role of a liquid compartment, which provides pronounced hydration effect and helps maintain a natural moist environment of the healing tissues. BioAquacare showed relatively low protein-absorbing activity, absorbing predominantly low-molecular-weight molecules, including interleukin (IL)-1beta, IL-6, transforming growth factor-beta1 and products of haemoglobin degradation. It is concluded that application of the moist BioAquacare dressing promotes fast reepithelialisation by creating favourable environment for keratinocytes proliferation and it also reduces scarring. The results show that BioAquacare can be considered as a safe, biocompatible and inflammatory inert wound dressing material. PMID:17199768

Shingel, Kirill I; Di Stabile, Liliana; Marty, Jean-Paul; Faure, Marie-Pierre

2006-12-01

108

Screening evaluation of an ionized nanocrystalline silver dressing in chronic wound care.  

PubMed

The successful topical treatment of chronic wounds requires adequate debridement, bacterial balance, and moisture balance. An ionized nanocrystalline silver dressing was evaluated through an uncontrolled, prospective study of a case series of 29 patients with a variety of chronic nonhealing wounds. The four arms of the study included nine patients with foot ulcers, six patients with venous stasis ulcers, two patients with pressure ulcers, and 12 patients with miscellaneous wounds. All wounds were assessed for the usual signs of clinical infection, with most of these parameters being measured and recorded. Microbiologically, bacterial load was determined via quantitative biopsies and semi-quantitative swabs. In general, the results showed a marked clinical improvement for the majority of wounds treated with the dressing. Among improved parameters included decreased exudate and decreased purulence. The quantitative bacterial biopsies did not show any decrease in organism numbers, although the semi-quantitative swabs indicated a decrease in the wound surface bacterial loading. This was indicative of the dressing's ability to reduce surface bacteria and achieve an element of bacterial balance in the superficial dermal compartment. The proposed mechanism of action for this ionized nanocrystalline based dressing is through bacterial and moisture balance within the superficial wound space compartment. PMID:11890077

Sibbald, R G; Browne, A C; Coutts, P; Queen, D

2001-10-01

109

Supramolecular elastomer based on polydimethylsiloxanes (SESi) film: synthesis, characterization, biocompatibility, and its application in the context of wound dressing.  

PubMed

Supramolecular elastomer based on polydimethylsiloxanes (SESi) is a kind of novel elastomer cross-linked by the multihydrogen bonds supplied by the functional groups linked to the end of the PDMS chains, such as amide, imidazolidone, pending urea (1,1-dialkyl urea), and bridging urea (1,3-dialkyl urea). SESi showed lower glass transition temperature (T g) at about -113?°C because of the softer chain of PDMS, and could show real rubber-like elastic behaviors and acceptable water vapor transmission rate under room temperature. The high biocompatibility of SESi in the form of films was demonstrated by the cytotoxicity evaluation (MTT cytotoxicity assay and direct contact assay), hemolysis assay, and skin irritation evaluation. Based on detailed comparisons between commercial Tegaderm(™) film and SESi film using a full-thickness rat skin model experiment, it was found that SESi film showed similar wound contraction rate as that of Tegaderm(™) film on day seven, 10, and 14; only on day five, SESi film showed a significant (p?wound contraction rate. And, the wounds covered with SESi film were filled with new epithelium without any significant adverse reactions, similar with that of Tegaderm(™) film. PMID:24073612

Zhang, Anqiang; Yang, Lin; Lin, Yaling; Lu, Hecheng; Qiu, Yuanhuan; Su, Yanlong

2013-01-01

110

Microbicidal properties of a silver-containing hydrofiber dressing against a variety of burn wound pathogens.  

PubMed

Partial-thickness burns are often characterized by microbial contamination and copious exudate produced during the early postburn period. Consequently, topical wound management often relies on the use of antimicrobial agents and absorbent dressings, and an AQUACEL Hydrofiber Dressing containing ionic silver has been designed to meet such needs. To assess the antimicrobial properties of the AQUACEL Hydrofiber dressing, samples were challenged with a wide variety of recognized burn wound pathogens in a simulated wound fluid model. Dressing samples were inoculated with the challenge organisms at time zero and then reinoculated on days 4 and 9 to mimic the worst-case clinical scenario. The dressing was shown to be microbicidal against aerobic and anaerobic bacteria (including antibiotic-resistant strains), yeasts, and filamentous fungi during a 14-day test period. Based on our results, the silver-containing dressing is likely to provide a barrier to infection, in addition to providing proven fluid-handling benefits of the AQUACEL Hydrofiber dressing, in the management of partial-thickness burns. PMID:15091147

Bowler, P G; Jones, S A; Walker, M; Parsons, D

2004-01-01

111

Introducing mixed-charge copolymers as wound dressing biomaterials.  

PubMed

Herein, a pseudozwitterionic structure bearing moieties with mixed positive and negative charges is introduced to develop a potential biomaterial for wound dressing applications. New mixed-charge matrices were prepared by copolymerization of the negatively charged 3-sulfopropyl methacrylate (SA) and positively charged [2-(methacryloyloxy)ethyl] trimethylammonium (TMA) onto expanded polytetrafluoroethylene (ePTFE) membranes. The charge balance was effectively regulated through the control of the initial SA/TMA ratio. The focus was then laid on the assessment of a variety of essential properties of efficient wound dressings including, hydration property, resistance to fibrinogen adsorption, hemocompatibility, as well as resistance to fibroblast attachment and bacteria colonization. It was found that the pseudozwitterionic membranes, compared to those with charge bias in the poly(SA-co-TMA) structure, exhibited the best combination of major properties. Therefore, they were further tested for wound healing. Histological examination of mouse wound treated with the pseudozwitterionic membranes exhibited complete re-epithelialization and total formation of new connective tissues after 14 days, even leading to faster healing than using commercial dressing. Results presented in this work suggest that the mixed-charge copolymers with a perfect balance of positive and negative moieties represent the newest generation of biomaterials for wound dressings. PMID:24881869

Jhong, Jheng-Fong; Venault, Antoine; Liu, Lingyun; Zheng, Jie; Chen, Sheng-Han; Higuchi, Akon; Huang, James; Chang, Yung

2014-06-25

112

Preparation and evaluation of biocomposites as wound dressing material.  

PubMed

Collagen was isolated from the chrome containing leather waste (CCLW) which is a major solid waste in leather industry. Composite films were made using sago starch (SG), soya protein (SY), and collagen (C) and were cross linked with glutaraldehyde (G).The films prepared were characterized for their physico chemical properties like tensile strength, infrared spectra, thermogravimetric analysis, surface morphology, and water absorption studies. Better mechanical properties and surface morphology were observed for SG-SY-G-C films compared to other films prepared using collagen. The composite films prepared were used as wound dressing material on the experimental wounds of rats and healing pattern was evaluated using planimetric, biochemical, and histopathological studies. These studies have revealed better wound healing capacity of SG-SY-G-C film and utilization of CCLW in the preparation of value added product like wound dressing material. PMID:23015263

Ramnath, V; Sekar, S; Sankar, S; Sankaranarayanan, C; Sastry, T P

2012-12-01

113

Preparation and evaluation of a novel wound dressing sheet comprised of ?-glucan–chitosan complex  

Microsoft Academic Search

A transparent wound dressing sheet was obtained by forming a complex between ?-glucan and chitosan (CS). These materials were chosen for their biocompatible, bioabsorbable, and biodegradable properties, and they were expected to promote the therapeutic efficacy of the dressing by increasing the wound healing response. The therapeutic efficacy of the ?-glucan–CS complex sheet as a wound dressing was evaluated in

Kyoko Kofuji; Yuzhou Huang; Kazufumi Tsubaki; Fumihiko Kokido; Kazunori Nishikawa; Takashi Isobe; Yoshifumi Murata

2010-01-01

114

Amniotic membrane extract-loaded double-layered wound dressing: evaluation of gel properties and wound healing.  

PubMed

Abstract The conservative single-layered wound dressing system is decomposed when mixed in polyvinyl alcohol (PVA) solution, which means it cannot be used with a temperature-sensitive drug. The goal of this investigation was to make an amniotic membrane extract (AME)-loaded double-layered wound dressing with an improved healing result compared to the conservative single-layered wound dressing systems. The double-layered wound dressing was developed with PVA/sodium alginate using a freeze-melting technique; one layer was PVA layer and the other was the drug-loaded sodium alginate layer. Its gel properties were assessed compared to single-layered wound dressings. Moreover, in vivo wound-healing effects and histopathology were calculated compared to commercial products. The double-layered wound dressing gave a similar gel fraction and Young's module as single-layered wound bandages developed with only PVA, and a similar inflammation ability and WVTR as single-layered wound dressings developed with PVA and sodium alginate. Our data indicate that these double-layered wound bandages were just as swellable, but more elastic and stronger than single-layered wound dressings comprised of the same polymers and quantities, possibly giving an acceptable level of moisture and accumulation of exudates in the wound zone. Compared to the commercial product, the double-layered wound dressing comprising 6.7% PVA, 0.5% sodium alginate and 0.01% AME significantly enhanced the wound-healing effect in the wound-healing test. Histological investigations showed that superior full-thickness wound-healing effects compared to the commercial product. Therefore, the double-layered wound dressing would be an outstanding wound-dressing system with improved wound healing and good gel property. PMID:23621769

Choi, Yeung Keun; Din, Fakhar Ud; Kim, Dong Wuk; Kim, Yong-Il; Kim, Jong Oh; Ku, Sae Kwang; Ra, Jeong-Chan; Huh, Jae-Wook; Lee, Jangik I; Sohn, Dong Hwan; Yong, Chul Soon; Choi, Han-Gon

2014-07-01

115

Hyperbranched polyglycerol electrospun nanofibers for wound dressing applications  

Microsoft Academic Search

This study reports on the performance of electrospun hyperbranched polyglycerol nanofibers capable of providing an active agent delivery for wound dressing applications. The aim of this work was to prepare electrospun HPGL nanofibers containing Calendula officinalis as a wound-healing and anti-inflammatory agent. The morphology of the electrospun HPGL–C. officinalis nanofibers was analyzed using a scanning electron microscope. The results showed

E. A. Torres Vargas; N. C. do Vale Baracho; J. de Brito; A. A. A. de Queiroz

2010-01-01

116

Permeability and Biocompatibility of Novel Medicated Hydrogel Wound Dressings  

Microsoft Academic Search

Hydrogel dressings are being popularized for wound care management because of their softness, tissue compatibility, and ability to enhance wound healing process. PVP-CMC and PVP-CMC-BA hydrogels were prepared using polyvinylpyrrolidone (PVP), sodium-carboxymethylcellulose (CMC), polyethyleneglycol (PEG), agar, glycerine and without\\/with boric acid (BA). Permeability: water vapor transmission and microbe penetration and biocompatibility: cytotoxicity, skin irritation, and skin sensitization tests of hydrogels

Niladri Roy; Nabanita Saha; Petr Humpolicek; Petr Saha

2010-01-01

117

Evaluation of boiled potato peel as a wound dressing.  

PubMed

In a series of experiments full thickness skin defects in 68 rats were covered with dressings made of boiled potato peels according to the method developed in Bombay. The wounds closed within 14 days and histologically complete repair of epidermis was found. The cork layer of the potato peel prevents dehydration of the wound and protects against exogenous agents. Experiments with homogenates revealed that a complete structure of the peel is necessary. Steroidal glycosides may have contributed to the favourable results. PMID:1930669

Dattatreya, R M; Nuijen, S; van Swaaij, A C; Klopper, P J

1991-08-01

118

Investigation of lysine acrylate containing poly(N-isopropylacrylamide) hydrogels as wound dressings in normal and infected wounds.  

PubMed

The design of materials for cutaneous wound dressings has advanced from passive wound covers to bioactive materials that promote skin regeneration and prevent infection. Crosslinked poly(N-isopropylacrylamide) (PNIPAAm)-based hydrogels have been investigated for a number of biomedical applications. While these materials can be used for drug delivery, limited cell interactions restrict their biological activity. In this article, acryoyl-lysine (A-Lys) was incorporated into poly(ethylene glycol) crosslinked PNIPAAm to enhance biological activity. A-Lys could be incorporated into the hydrogels to improve cellular interaction in vitro, while maintaining swelling properties and thermoresponsive behavior. Polyhexamethylene biguanide, an antimicrobial agent, could be encapsulated and released from the hydrogels and resulted in decreased bacteria counts within 2 hours. Two in vivo animal wound models were used to evaluate the hydrogel wound dressing. First, application of the hydrogels to a rodent cutaneous wound healing model resulted in significant increase in healing rate when compared with controls. Moreover, the hydrogels were also able to decrease bacteria levels in an infected wound model. These results suggest that PNIPAAm hydrogels containing A-Lys are promising wound dressings due to their ability to promote healing and deliver active antimicrobial drugs to inhibit infection. PMID:22121043

Jiang, Bin; Larson, Jeffery C; Drapala, Pawel W; Pérez-Luna, Víctor H; Kang-Mieler, Jennifer J; Brey, Eric M

2012-04-01

119

Subperiosteal behaviour of alginate and cellulose wound dressing materials  

Microsoft Academic Search

A histological comparison was undertaken of the tissue response to a new sodium calcium alginate material (Kaltostat®) and an oxidized regenerated cellulose wound dressing (Surgicel®) when implanted between bone and periosteum in the jaws at intervals of up to 24 wk. Both biomaterials caused a foreign body reaction, persisting up to 12 wk after surgery. New bone formation occurred along

Ian R. Matthew; Roger M. Browne; John W. Frame; Brian G. Millar

1995-01-01

120

Influencing dressing choice and supporting wound management using remote 'tele-wound care'.  

PubMed

This article describes a local involvement in a project to evaluate a remote system of wound management, incorporating the use of digital and mobile technology. It outlines how this involvement influenced the current system of 'tele wound care' (remote wound management) in a large community organisation. The system allows remote wound assessment, management advice and ongoing monitoring of wounds to ensure that the dressing choice remains appropriate and that timely wound care support can be provided to community nurses, practice nurses and GPs. PMID:24912832

King, Brenda

2014-06-01

121

Modern Wound Dressings: A Systematic Approach to Wound Healing  

Microsoft Academic Search

The advent of modern wound care management constitutes one of the most innovative applications of medical device technology. The foundation for wound care recent advances has been built upon the developments achieved in polymer technology over the last three decades. New and unique materials have been engineered to provide properties with significant technical and clinical benefits.These new wound care products

Michael Szycher; Steven James Lee

1992-01-01

122

Fabrication and characterization of an asymmetric polyurethane membrane for use as a wound dressing  

Microsoft Academic Search

To prevent wound dehydration and bacterial penetration, a wound dressing should be occlusive, but on the other hand it should also be permeable for wound exudate to prevent bullae formation. To meet these requirements a new type of polyurethane wound dressing which consists of a microporous top layer (pore size < 0.7 m) supported by a sublayer with a highly

W. L. J. Hinrichs; E. J. C. M. P. Lommen; Ch. R. H. Wildevuur; J. Feijen

1992-01-01

123

Neurotensin-loaded collagen dressings reduce inflammation and improve wound healing in diabetic mice.  

PubMed

Impaired wound healing is an important clinical problem in diabetes mellitus and results in failure to completely heal diabetic foot ulcers (DFUs), which may lead to lower extremity amputations. In the present study, collagen based dressings were prepared to be applied as support for the delivery of neurotensin (NT), a neuropeptide that acts as an inflammatory modulator in wound healing. The performance of NT alone and NT-loaded collagen matrices to treat wounds in streptozotocin (STZ) diabetic induced mice was evaluated. Results showed that the prepared dressings were not-cytotoxic up to 72h after contact with macrophages (Raw 264.7) and human keratinocyte (HaCaT) cell lines. Moreover, those cells were shown to adhere to the collagen matrices without noticeable change in their morphology. NT-loaded collagen dressings induced faster healing (17% wound area reduction) in the early phases of wound healing in diabetic wounded mice. In addition, they also significantly reduced inflammatory cytokine expression namely, TNF-? (p<0.01) and IL-1? (p<0.01) and decreased the inflammatory infiltrate at day 3 post-wounding (inflammatory phase). After complete healing, metalloproteinase 9 (MMP-9) is reduced in diabetic skin (p<0.05) which significantly increased fibroblast migration and collagen (collagen type I, alpha 2 (COL1A2) and collagen type III, alpha 1 (COL3A1)) expression and deposition. These results suggest that collagen-based dressings can be an effective support for NT release into diabetic wound enhancing the healing process. Nevertheless, a more prominent scar is observed in diabetic wounds treated with collagen when compared to the treatment with NT alone. PMID:24161538

Moura, Liane I F; Dias, Ana M A; Suesca, Edward; Casadiegos, Sergio; Leal, Ermelindo C; Fontanilla, Marta R; Carvalho, Lina; de Sousa, Hermínio C; Carvalho, Eugénia

2014-01-01

124

Morphology, drug release, antibacterial, cell proliferation, and histology studies of chamomile-loaded wound dressing mats based on electrospun nanofibrous poly(?-caprolactone)/polystyrene blends.  

PubMed

For the first time, it has been tried to achieve optimum conditions for electrospun poly(?-caprolactone)/polystyrene (PCL/PS) nanofibrous samples as active wound dressings containing chamomile via D-optimal design approach. In this work, systematic in vitro and in vivo studies were carried out by drug release rate, antibacterial and antifungal evaluations, cell culture, and rat wound model along with histology observation. The optimized samples were prepared under the following electrospinning conditions: PCL/PS ratio (65/35), PCL concentration 9%(w/v), PS concentration 14%(w/v), distance between the syringe needle tip and the collector 15.5 cm, applied voltage 18 kV, and solution flow rate 0.46 mL h(-1) . The FE-SEM micrographs showed electrospun PCL/PS (65/35) nanofibrous sample containing 15% chamomile had a minimum average diameter (?175 nm) compared to the neat samples (?268 nm). The drug released resulted in a gradual and high amount of chamomile from the optimized PCL/PS nanofibrous sample (?70%) in respect to PCL and PS nanofibers after 48 h. This claim was also confirmed by antibacterial and antifungal evaluations in which an inhibitory zone with a diameter of about 7.6 mm was formed. The rat wound model results also indicated that the samples loaded with 15% chamomile extract were remarkably capable to heal the wounds up to 99?±?0.5% after 14 days post-treatment periods. The adhesion of mesenchymal stem cells and their viability on the optimized samples were confirmed by MTT analysis. Also, the electrospun nanofibrous mats based on PCL/PS (65/35) showed a high efficiency in the wound closure and healing process compared to the reference sample, PCL/PS nanofibers without chamomile. Finally, the histology analysis revealed that the formation of epithelial tissues, the lack of necrosis and collagen fibers accumulation in the dermis tissues for the above optimized samples. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 977-987, 2014. PMID:24259351

Motealleh, Behrooz; Zahedi, Payam; Rezaeian, Iraj; Moghimi, Morvarid; Abdolghaffari, Amir Hossein; Zarandi, Mohammad Amin

2014-07-01

125

Effect of Hydrofiber wound dressings on bacterial ultrastructure.  

PubMed

Ionic silver has well-proven bactericidal properties, and silver-containing wound dressings are now widely used to aid in the creation of an antimicrobial environment in wounds. The effect of silver ions on bacterial ultrastructure can best be studied by viewing bacterial cells under a transmission electron microscope (TEM). Bacterial cells of Pseudomonas aeruginosa were incubated within a control dressing (e.g. a non-antimicrobial Hydrofiber dressing) (Hydrofiber is a registered trademark of E.R. Squibb and Sons, L.L.C.) and a silver-containing Hydrofiber dressing, followed by processing for TEM. Liquid cultures, with and without silver, were prepared for comparison. The addition of silver to growing bacterial cultures stopped growth of the cells very quickly. Ultrastructurally, the presence of silver was found to affect both the shape of the bacterial nucleoid and the organization of bacterial DNA. X-ray microanalysis of bacteria from liquid cultures showed the presence of silver within silver-treated cells and the absence of calcium. It is suggested that the presence of available silver ions within the Hydrofiber dressing could lead to the loss of cellular ions, vital for maintaining the structural integrity of the nuclear area. PMID:18281349

Hobot, Jan; Walker, Michael; Newman, Geoffrey; Bowler, Philip

2008-04-01

126

Porous Collagen Sponge Wound Dressings: in vivo and in vitro Studies  

Microsoft Academic Search

Collagen-based materials can be formed into a three-dimensional sponge for use as a wound dressing and as a support for cell cultured skin components. Factors such as biocompatibility, morphological structure and addition of non-collagenous molecules to collagen are analyzed and discussed. Large pores or channels, interchannel communications and combinations of macromolecules of the connective tissue enhance wound tissue infiltration in

Charles J. Doillon

1987-01-01

127

The influence of dressings on the healing of normal and ischaemic wounds and flap survival.  

PubMed

The effects of dressing with Duoderm (occlusive hydrocolloid) and Mepore (permeable viscose) on the healing of normal and ischaemic incisional wounds, and on flap survival, were investigated in 60 rats. The biomechanical properties of dressed normal wounds after 14 days did not differ from those of the undressed controls. In contrast, energies at maximum and breaking (load*S, stress*S) of dressed ischaemic wounds decreased by 30%-42% after 14 days of healing, compared with undressed ischaemic controls. Dressing decreased the shrinkage of ischaemic wounds and necrosis length of ischaemic flaps. Normal incisional wounds can safely be dressed for 14 days without the wound strength being affected. Dressings may be useful clinically in preventing superficial dermal necroses. One must, however, be aware of the impairment of the wound strength of ischaemic incisional wounds. PMID:11291341

Quirinia, A; Viidik, A

2001-03-01

128

Human neutrophil elastase and collagenase sequestration with phosphorylated cotton wound dressings.  

PubMed

The design and preparation of wound dressings that redress the protease imbalance in chronic wounds is an important goal of wound healing and medical materials science. Chronic wounds contain high levels of tissue and cytokine-destroying proteases including matrix metalloprotease and neutrophil elastase. Thus, the lowering of excessive protease levels in the wound environment by wound dressing sequestration prevents the breakdown of extracellular matrix proteins and growth factors necessary for wound healing. Phosphorylated cotton wound dressings were prepared to target sequestration of proteases from chronic wound exudate through a cationic uptake binding mechanism involving salt bridge formation of the positively charged amino acid side chains of proteases with the phosphate counterions of the wound dressing fiber. Dressings were prepared by applying sodium hexametaphosphate and diammonium phosphate in separate formulations to cotton gauze by pad/dry/cure methods. Phosphorylated cotton dressings were assessed for their ability to lower elastase and collagenase activity. The phosphorylated cotton dressings lowered elastase and collagenase activity 40-80% more effectively than the untreated cotton wound dressings under conditions that mimic chronic wound exudate. Efficacy of the phosphorylated cotton was found to be related to the level of phosphorylation and a lower pH due to protonated phosphate at the surface of the dressing. The capacity of the modified gauze to sequester continued elastase secretions similar to that found in a chronic wound over a 24-h period was retained within a 80% retention of elastase sequestration and was dose-dependent. PMID:17477392

Edwards, J Vincent; Howley, Phyllis S

2007-11-01

129

Chitin membranes containing silver nanoparticles for wound dressing application.  

PubMed

Silver nanoparticles are gaining importance as an antimicrobial agent in wound dressings. Chitin is a biopolymer envisioned to promote rapid dermal regeneration and accelerate wound healing. This study was focused on the evaluation of chitin membranes containing silver nanoparticles for use as an antimicrobial wound dressing. Silver nanoparticles were synthesised by gamma irradiation at doses of 50 kGy in the presence of sodium alginate as stabiliser. The UV-Vis absorption spectra of nanoparticles exhibited an absorption band at 415-420 nm, which is the typical plasmon resonance band of silver nanoparticles. The peaks in the X-ray diffraction (XRD) pattern are in agreement with the standard values of the face-centred cubic silver. Transmission electron microscopy (TEM) images indicate silver nanoparticles with spherical morphology and small particle size in the range of 3-13 nm. In vitro antimicrobial tests were performed using Pseudomonas aeruginosa and Staphylococcus aureus to determine the antimicrobial efficiency of the chitin membranes containing 30, 50, 70 and 100 ppm nanosilver. No viable counts for P. aeruginosa were detected with 70 ppm silver nanoparticles dressing after 1-hour exposure. A 2-log reduction in viable cell count was observed for S. aureus after 1 hour and a 4-log reduction after 6 hours with 100 ppm nanosilver chitin membranes. This study demonstrates the antimicrobial capability of chitin membranes containing silver nanoparticles. The chitin membranes with 100 ppm nanosilver showed promising antimicrobial activity against common wound pathogens. PMID:22958740

Singh, Rita; Singh, Durgeshwer

2014-06-01

130

Physico-mechanical properties of wound dressing material and its biomedical application  

Microsoft Academic Search

A bioadhesive wound dressing material, based on gelatin, was prepared by solution casting, and its properties were evaluated. The tensile strength (TS) and percentage elongation at break (Eb) of the membranes were found to be 12.7 MPa and 40.4%, respectively. The buffer uptake and water uptake of the prepared membranes were found to be 298 and 312%, respectively, after 8

Haydar U. Zaman; J. M. M. Islam; Mubarak A. Khan; Ruhul A. Khan

2011-01-01

131

Clinical application of chitin non-woven fabric as wound dressing  

Microsoft Academic Search

A new wound dressing, chitin non-woven fabric was applied in 91 patients for dressing of donor sites, skin graft areas, raw areas under pedicle flaps and those associated with skin abrasion, as well as burns and skin ulcers. Chitin dressing showed excellent results with regard to satisfactory pain relief, adherence to the wound and drying without dissolution or other adverse

Y. Ohshima; K. Nishino; Y. Yonekura; S. Kishimoto; S. Wakabayashi

1987-01-01

132

Clinical and histological evaluation of two dressing materials in the healing of palatal wounds  

PubMed Central

Background Free gingival grafts have been used extensively for gingival augmentation procedures, but are associated with postoperative morbidity because of the open palatal wound. This study compares the clinical efficiency of two dressing materials, a non-eugenol-based dressing (Coe-Pak™) and a collagen dressing (Colla Cote®) on palatal wound healing. Materials and Methods Thirty-two patients in the age group of 25?50 years, who required gingival augmentation, were selected. Free gingival graft was harvested from the palatal mucosa and the wound was then protected using Coe-pak® in control group and Colla Cote® in test group. The subjective parameters pain and burning sensation were recorded on the 2nd and 7th day and the objective parameters colour and consistency were recorded on the 7th and 42nd day, using a visual analog scale. Thickness of the mucosa was measured using K file at baseline and 42nd day. Histological examination was done on 42nd day. Results The subjective and objective parameters showed significant improvement in the test group when compared to control group. Histologically, there was a greater evidence of collagen formation and turn over in the test group than control group. Conclusions Collagen-based dressing may thus offer significantly greater advantages over the traditional non-eugenol dressings.

Shanmugam, M.; Kumar, T.S.S.; Arun, K.V.; Arun, Ramya; Karthik, S. Jai

2010-01-01

133

Dressing plantar wounds with foam dressings, is it too much pressure?  

PubMed Central

Diabetes and its associated complications have become a major concern locally, nationally and internationally. One such complication is lower extremity amputation, commonly preceded by chronic ulceration. The cause of this tissue breakdown is multi-faceted, but includes an increase in pressure, particularly plantar pressure. As such, the choice of dressing to be applied to a plantar wound should ideally not increase this pressure further. A commonly used and possibly more bulky dressing is the foam dressing. This pilot study investigates the plantar pressures associated with three common foam dressings (Allevyn®, Lyofoam® and Mepilex®) compared with a control dressing (Melolin®). Twelve healthy males and 19 females [SD] age 36.6 [10.4] were measured using the F-scan plantar pressure measurement system. Substantial variations in individual pressure changes occurred across the foot. No significant differences were identified, once a Bonferroni correction was applied. In healthy adults, it could be concluded that foam dressings do not have any effect on the plantar pressures of the foot. However, the need remains for a robust trial on a pathological population.

Scott Causby, Ryan; Pod, M; Jones, Sara

2011-01-01

134

Efficiency of Microbial Cellulose Dressing in Partial Thickness Burn Wounds  

Microsoft Academic Search

Microbial cellulose is a natural polymer that can hold an quantity of water without any disconformities. As a result it is considered to be a wound-dressing material. We report a case of 28% total body surface area partial-thickness flame burn, approximately 4.5% superficial partial-thickness burns on anterior face, and 23.5% mixed, between superficial and deep partial-thickness burns, on both upper

Pornprom Muangman; Supaporn Opasanon; Supaparn Suwanchot; Orapin Thangthed

135

Preparation of acrylic grafted chitin for wound dressing application  

Microsoft Academic Search

Chitin grafted with poly(acrylic acid) (chitin–PAA) was prepared with the aim of obtaining a hydrogel characteristic for wound dressing application. The chitin–PAA films were synthesized at various acrylic acid feed contents to investigate its effect on water sorption ability. Acrylic acid (AA) was first linked to chitin, acting as the active grafting sites on the chain that was further polymerized

Siriporn Tanodekaew; Malinee Prasitsilp; Somporn Swasdison; Boonlom Thavornyutikarn; Thanawit Pothsree; Rujiporn Pateepasen

2004-01-01

136

Biocompatibility study for PVP wound dressing obtained in different conditions  

NASA Astrophysics Data System (ADS)

Hydrogels composed of PVP, PEG and agar, produced by simultaneous crosslinking and package sterilization by ionizing radiation, are used mainly as wound dressing. In this study, membranes prepared in different conditions were tested for their properties including in vitro biocompatibility. The results showed that the mechanical properties were in an acceptable range of values and that the membranes can be considered as non toxic and non hemolytic to the cells.

Higa, O. Z.; Rogero, S. O.; Machado, L. D. B.; Mathor, M. B.; Lugão, A. B.

1999-08-01

137

Do Functional Keratin Dressings Accelerate Epithelialization in Human Partial Thickness Wounds? A Randomized Controlled Trial on Skin Graft Donor Sites  

PubMed Central

Objective: To determine if the experimental (keratin-based) dressing accelerates epithelialization rates during healing of partial-thickness wounds, relative to a Standard Care dressing. Method: A randomized control trial was conducted using a Standard Care dressing side by side with the experimental dressing on a sample (n=26) of partial-thickness donor site wounds. The proximal/distal placement of the control and treatment was randomized. Percentage epithelialization after approximately 7 days was estimated from which time to fully epithelialize can be inferred. Patients were grouped into “young” (?50 y/o) and “old” (>50 y/o). Results: For the “old” patients (n=15), the median epithelialization percentage at 7 days is 5% and was significantly (P=.023) greater for the experimental dressing. For the “young” patients (n=11), the median epithelialization percentage at 7 days was 80% and there is no significant difference between the experimental and Standard Care control dressings. Conclusions: The experimental dressing significantly increases the rate of epithelialization of acute, traumatic partial-thickness wounds in older patients. We suggest that the dressing may be clinically useful in similar situations where epithelialization may be delayed because of patient or wound characteristics.

Davidson, Andrew; Jina, N. Hamesh; Marsh, Clive; Than, Martin; Simcock, Jeremy W.

2013-01-01

138

A pre-clinical evaluation of silver, iodine and Manuka honey based dressings in a model of traumatic extremity wounds contaminated with Staphylococcus aureus.  

PubMed

Prevention of extremity war wound infection remains a clinical challenge. Staphylococcus aureus is the most common pathogen in delayed infection. We hypothesised that choice of wound dressings may affect bacterial burden over 7 days reflecting the current practice of delayed primary closure of wounds within this timeframe. A randomised controlled trial of 3 commercially available dressings (Inadine(®) (Johnson & Johnson, NJ, USA), Acticoat(®) (Smith & Nephew, Hull, UK), Activon Tulle (Advancis Medical, Nottingham, UK)) was conducted in a rabbit model of contaminated forelimb muscle injury. A positive control group treated with antibiotics was included. Groups were compared to a saline soaked gauze control. The primary outcome was a statistically significant reduction (p<0.05) in tissue S. aureus at 7 days post-injury. Secondary outcome measurements included bacteraemias, observational data, whole blood determination, ELISA for plasma biomarkers, PCR array analysis of wound healing gene expression and muscle/lymph node histopathology. Antibiotic, Inadine and Acticoat groups had statistically significant lower bacterial counts (mean 7.13 [95% CI 0.00-96.31]×10(2); 1.66 [0.94-2.58]×10(5); 8.86 [0.00-53.35]×10(4)cfu/g, respectively) and Activon Tulle group had significantly higher counts (2.82 [0.98-5.61]×10(6)cfu/g) than saline soaked gauze control (7.58 [1.65-17.83]×10(5)cfu/g). There were no bacteraemias or significant differences in observational data or whole blood determination. There were no significant differences in muscle/loss or pathology and lymph node cross-sectional area or morphology. There were some significant differences between treatment groups in the plasma cytokines IL-4, TNF? and MCP-1 in comparison to the control. PCR array data demonstrated more general changes in gene expression in the muscle tissue from the Activon Tulle group than the Inadine or Acticoat dressings with a limited number of genes showing significantly altered expression compared to control. This study has demonstrated that both Acticoat(®) and Inadine(®) dressings can reduce the bacteria burden in a heavily contaminated soft tissue wound and so they may offer utility in the clinical setting particularly where surgical treatment is delayed. PMID:24908627

Guthrie, Hugo C; Martin, Kevin R; Taylor, Christopher; Spear, Abigail M; Whiting, Rachel; Macildowie, Sara; Clasper, Jonathan C; Watts, Sarah A

2014-08-01

139

An active wound dressing for controlled convective mass transfer with the wound bed.  

PubMed

Conventional wound dressings-gauze, plastic films, foams, and gels-do not allow for spatial and temporal control of the soluble chemistry within the wound bed, and are thus limited to a passive role in wound healing. Here, we present an active wound dressing (AWD) designed to control convective mass transfer with the wound bed; this mass transfer provides a means to tailor and monitor the chemical state of a wound and, potentially, to aid the healing process. We form this AWD as a bilayer of porous poly(hydroxyethyl methacrylate) (pHEMA) and silicone; the pHEMA acts as the interface with the wound bed, and a layer of silicone provides a vapor barrier and a support for connecting to external reservoirs and pumps. We measure the convective permeability of the pHEMA sponge, and use this value to design a device with a spatially uniform flow profile. We quantify the global coefficient of mass transfer of the AWD on a dissolvable synthetic surface, and compare it to existing theories of mass transfer in porous media. We also operate the AWD on model wound beds made of calcium alginate gel to demonstrate extraction and delivery of low molecular weight solutes and a model protein. Using this system, we demonstrate both uniform mass transfer over the entire wound bed and patterned mass transfer in three spatially distinct regions. Finally, we discuss opportunities and challenges for the clinical application of this design of an AWD. PMID:17106898

Cabodi, Mario; Cross, Valerie L; Qu, Zheng; Havenstrite, Karen L; Schwartz, Suzanne; Stroock, Abraham D

2007-07-01

140

[Clinical experiences with a new gel-like wound dressing after skin transplantation].  

PubMed

Free split skin grafting is now widely practised on a routine basis in accident surgery. All conventional wound dressing methods have considerable drawbacks, and this is one of the reasons why successful skin grafting is still an unsafe procedure even under the very best of conditions and quite often resembles an experiment more than a scientific procedure, with an unpredictable outcome. Today, however, we can command over a wound dressing, using the polyacrylamide agar hydrogel developed by the Max Planck Institute of Immunobiology at Freiburg and which is being marketed under the trade name of Geliperm. This dressing can maintain the physiological wound environment during the first few critical days following grafting; it enables conditioning of the wound; it protects the wound against bacterial invasion and prevents drying-out of the graft and the base of the wound; and it is capable of absorbing wound secretion to a certain degree. Healing chances of the graft can be considerably improved by the use of Geliperm. Thanks to its high measure of elasticity it can adapt itself to the wound surface without sticking to it. The transparent nature of the material enables to observe and assess the healing processes at any time and to recognize possible complications during healing. Failures are almost always due to prolonged deposition of the gel plates, but this can be safely avoided by regularly changing the deposit. Deposits inadvertently left for too long, so that they have started drying at the wound surface, can be detached without any trouble and painlessly without any risk of damaging the graft.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:6151801

Knapp, U; Rahn, H D; Schauwecker, F

1984-12-01

141

Silicone dressings are a good fit in the wound care jigsaw.  

PubMed

The dressings that are available for clinicians to use in wound care are varied, each with their own indications, so where does 'silicone' fit in? Reports of wound pain are common, particularly during dressing changes, but pain can also be continuously experienced between dressing changes. There are also concerns about trauma to the peri-wound during dressing removal, which causes 'skin stripping', leading to further pain and delayed healing. The use of silicone dressings can help to reduce these occurrences in practice. PMID:23587970

Yarwood-Ross, Lee

142

Electrospun non-woven nanofibrous hybrid mats based on chitosan and PLA for wound-dressing applications.  

PubMed

Continuous defect-free nanofibers containing chitosan (Ch) or quaternized chitosan (QCh) were successfully prepared by one-step electrospinning of Ch or QCh solutions mixed with poly[(L-lactide)-co-(D,L-lactide)] in common solvent. XPS revealed the surface chemical composition of the bicomponent electrospun mats. Crosslinked Ch- and QCh-containing nanofibers exhibited higher kill rates against bacteria S. aureus and E. coli than the corresponding solvent-cast films. SEM observations showed that hybrid mats were very effective in suppressing the adhesion of pathogenic bacteria S. aureus. The hybrid nanofibers are promising for wound-healing applications. PMID:18855947

Ignatova, Milena; Manolova, Nevena; Markova, Nadya; Rashkov, Iliya

2009-01-01

143

In vitro microbial inhibition and cellular response to novel biodegradable composite wound dressings with controlled release of antibiotics.  

PubMed

About 70% of all people with severe burns die from related infections, despite advances in treatment regimens and the best efforts of nurses and doctors. Although silver-eluting wound dressings are available for addressing this problem, there is growing evidence of the deleterious effects of such dressings in delaying the healing process owing to cellular toxicity. A new concept of antibiotic-eluting composite wound dressings is described here. These dressings are based on a polyglyconate mesh coated with a porous poly-(dl-lactic-co-glycolic acid) matrix loaded with antibiotic drugs. The effect of antibiotic release on bacterial inhibition was studied, and cell cytotoxicity was examined. The dressings resulted in a 99.99% decrease in the viable counts of Pseudomonas aeruginosa and Staphylococcus albus at very high initial inoculations of 10?-10? CFU ml?¹ after only 1 day, while such a decrease in Staphylococcus aureus was obtained within 3 days. Bacterial inhibition zones around the dressing material were found to persist for 2 weeks, indicating a long-lasting antimicrobial effect. Despite severe toxicity to bacteria, the dressing material was found to have no toxic effect on cultured fibroblasts, indicating that the new antibiotic-eluting wound dressings represent an effective option for selective treatment of bacterial infections. PMID:20643231

Elsner, J J; Berdicevsky, I; Zilberman, M

2011-01-01

144

Evaluation of a bi-layer wound dressing for burn care  

Microsoft Academic Search

Severe burns remain a significant cause of morbidity and mortality despite the availability of numerous therapies. We assessed the wound healing and skin-cooling properties of a DRDC hydrogel\\/polyurethane wound dressing using different pre-clinical models. Our results show that 85% of partial-thickness, non-contaminated porcine wounds treated with our dressing healed within 6 days. In contrast, 85% of the wounds treated with

Lucie Martineau; Pang N. Shek

2006-01-01

145

Application of wound dressing Molndal technique in clean and potentially contamined postoperative wounds--initial comparative study.  

PubMed

Because of a possible delayed wound healing, critical colonization and infection of wounds present a problem for surgeons, particularly in patients with compromised immune system or in case where the wound is heavy contaminated or poorly perfused. Molndal technique of wound dressing has proven to be effective in prevention of infection. In our study we wanted to describe the benefits of the application of Molndal technique wound dressing compared to traditional wound dressing technique at potentially contaminated and clean postoperative wounds. We examined postoperative wound after radical excision of pilonidal sinus and after implantation of partial endoprosthesis in hip fracture. Molndal technique consisted of wound dressing with Aquacel Ag - Hydrofiber. Traditional technique was performed using gauze compresses and hypoallergic adhesives. We analyzed the results of 50 patients after radical excision of pilonidal sinus. 25 patients were treated by Molndal technique and 25 patients by the traditional technique of wound dressing. In the group treated by Molndal technique only 1 (4%) patient has revealed a wound infection, proven by positive microbiological examination and suppuration. In the traditional technique group 4 (16%) patients developed wound infection as inflammation and secretion as a sign of superficial infection. In the other group we analyzed the results of 50 patients after implantation of partial endoprosthesis after hip fracture. 20 patients were treated by Molndal technique and 30 patients by the traditional technique of wound dressing. In the group treated by Molndal technique no patient has revealed a wound infection (0%). In the traditional technique group 4 (13%) patients developed wound infection. All complication in both group were superficial incisional surgical infection (according to HPSC). There was no deep incisional surgical site infection or organ/space surgical site infection. Our results are clearly showing that Molndal technique is effective in preventing the postoperative wound infection. PMID:22220414

Marinovi?, Marin; Cicvari?, Tedi; Grzalja, Nikola; Baci?, Giordano; Radovi?, Endi

2011-09-01

146

Multispecies biofilm in an artificial wound bed-A novel model for in vitro assessment of solid antimicrobial dressings.  

PubMed

Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48h post-transplant. The applicability of the model was evaluated by testing two common iodine wound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant. PMID:24880129

Kucera, J; Sojka, M; Pavlik, V; Szuszkiewicz, K; Velebny, V; Klein, P

2014-08-01

147

In vitro diffusion bed, 3-day repeat challenge 'capacity' test for antimicrobial wound dressings  

Microsoft Academic Search

The aim of this study was to develop an in vitro wound infection model that allows the comparison of the bacterial kill rate of antimicrobial wound dressings over the course of 3 days, with renewed microbial challenges each day, under realistic wound-like conditions. A test bed model of a moderately exuding wound was constructed from a hydrogel containing releasable foetal

John Greenman; Saliah Saad; Andrew J Austin

2006-01-01

148

Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing  

Microsoft Academic Search

Bacterial cellulose was produced by Acetobacter xylinum (strain TISTR 975). Bacterial cellulose is an interesting material for using as a wound dressing since it provides moist environment to a wound resulting in a better wound healing. However, bacterial cellulose itself has no antimicrobial activity to prevent wound infection. To achieve antimicrobial activity, silver nanoparticles were impregnated into bacterial cellulose by

Thawatchai Maneerung; Seiichi Tokura; Ratana Rujiravanit

2008-01-01

149

Histological and bacteriological studies of burn wounds treated with boiled potato peel dressings.  

PubMed

Histological and clinical studies have been made on comparable burn wounds covered with either boiled potato peels affixed to gauze bandages or gauze dressings alone; both dressings were applied over a thin layer of 5 per cent silver sulphadiazine. Compared with treatment with plain gauze dressings, the application of the potato peel dressing reduced or eliminated dessication, permitted the survival of superficial skin cells and hastened epithelial regeneration. Bacteriological studies showed that the potato peels had no intrinsic antibacterial activity, the wounds beneath both dressings showing either no growth or, on most occasions, the same bacterial species. The easy availability of potato peels and gauze bandages on to which they can be affixed, the simplicity of the preparation of this dressing, the ease of sterilization and its low cost of production make this the dressing of choice for burn wounds in our developing country. PMID:2350410

Keswani, M H; Vartak, A M; Patil, A; Davies, J W

1990-04-01

150

The boiled potato peel as a burn wound dressing: a preliminary report.  

PubMed

This paper discusses the suitability of potato peel as a burn wound dressing in developing countries. Clinical trials have demonstrated that epithelial growth occurs under the potato peel dressing in superficial partial thickness skin loss burns. In deep partial, full skin thickness burns and in the late granulating burn wound the results are not so favourable. More experience is required. PMID:3986648

Keswani, M H; Patil, A R

1985-02-01

151

Biological wound dressings sterilized with gamma radiation: Mexican clinical experience  

NASA Astrophysics Data System (ADS)

Biological wound dressings sterilized with gamma radiation, such as amnion and pig skin, are a reality in Mexico. These tissues are currently processed in the tissue bank and sterilized in the Gamma Industrial Irradiation Plant; both facilities belong to the Instituto Nacional de Investigaciones Nucleares (ININ) (National Institute of Nuclear Research). With the strong support of the International Atomic Energy Agency, the bank was established at the ININ and the Mexican Ministry of Health issued its sanitary license on July 7, 1999. The Quality Management System of the bank was certified by ISO 9001:2000 on August 1, 2003; the scope of the system is "Research, Development and Processing of Biological Tissues Sterilized with Gamma Radiation". At present, more than 150 patients from 16 hospitals have been successfully treated with these tissues. This paper presents a brief description of the tissue processing, as well as the present Mexican clinical experience with children and adult patients who underwent medical treatment with radiosterilized amnion and pig skin, used as biological wound dressings on burns and ocular surface disorders.

Martínez-Pardo, M. E.; Ley-Chávez, E.; Reyes-Frías, M. L.; Rodríguez-Ferreyra, P.; Vázquez-Maya, L.; Salazar, M. A.

2007-11-01

152

Development of An Ultra-Fast-Curing Wound Dressing. Annual Report October 1, 1985 - June 30, 1986.  

National Technical Information Service (NTIS)

A drug-dispensing field wound dressing is being developed. The wound dressing, which can be easily applied by an untrained person, contains a coagulant to stop bleeding, and an antibiotic to prevent bacterial infection. The medicated wound dressing is mad...

M. Szycher J. L. Rolfe

1986-01-01

153

21 CFR 878.4015 - Wound dressing with poly (diallyl dimethyl ammonium chloride) (pDADMAC) additive.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Wound dressing with poly (diallyl dimethyl ammonium...Surgical Devices § 878.4015 Wound dressing with poly (diallyl dimethyl ammonium... (a) Identification . A wound dressing with pDADMAC additive is intended...

2010-04-01

154

Hydrogel–elastomer composite biomaterials: 4. Experimental optimization of hydrogel–elastomer composite fibers for use as a wound dressing  

Microsoft Academic Search

We report a novel 3-D cavity wound dressing based on a hydrogel–elastomer Interpenetrating Polymer Network (IPN) fabricated\\u000a into an open-mesh architecture. IPN fibers used to form the dressing were produced by a wet spinning method and optimized\\u000a in two steps. A factorial experiment was first conducted to identify key parameters that controlled fiber properties. We observed\\u000a that gelatin wt% played

Henry T. Peng; Lucie Martineau; Andy Hung

2008-01-01

155

Synthesis and properties of waterborne polyurethane hydrogels for wound healing dressings.  

PubMed

To accomplish ideal wound healing dressing, a series of waterborne polyurethane (WBPU) hydrogels based on polyethylene glycol (PEG) were synthesized by polyaddition reaction in an emulsion system. The stable WBPU hydrogels which have remaining weight of above 85% were obtained. The effect of the soft segment (PEG) content on water absorbability of WBPU hydrogels was investigated. Water absorption % and equilibrium water content (%) of the WBPU hydrogel significantly increased in proportion to PEG content and the time of water-immersion. The maximum water absorption % and equilibrium water content (%) of WBPU hydrogels containing various PEG contents were in the range of 409-810% and 85-96%, respectively. The water vapor transmission rate of the WBPU hydrogels was found to be in the range of 1490-3118 g/m(2)/day. These results suggest that the WBPU hydrogels prepared in this study may have high potential as new wound dressing materials, which provide and maintain the adequate moist environment required to prevent scab formation and dehydration of the wound bed. By the wound healing evaluation using full-thickness rat model experiment, it was found that the wound covered with a typical WBPU hydrogel (HG-78 sample) was completely filled with new epithelium without any significant adverse reactions. PMID:17973247

Yoo, Hye-Jin; Kim, Han-Do

2008-05-01

156

Impact of Silver-Containing Wound Dressings on Bacterial Biofilm Viability and Susceptibility to Antibiotics during Prolonged Treatment ?  

PubMed Central

The long-term antimicrobial efficacy of silver dressings against bacterial biofilms was investigated in a 7-day treatment in vitro model where the protein-rich medium was refreshed daily in order to mimic the conditions found in a wound bed. The use of plate-to-plate transfer assays demonstrated measurable differences in the effectivenesses of several silver dressings on the viability of biofilm bacteria and their susceptibility to antibiotics. Whereas after the first day of treatment, all dressings used resulted in a significant reduction in the number of viable cells in the biofilms and disruption of the biofilm colonies, during prolonged treatment, the efficacy of dressings with hydrophilic base materials diminished with daily transfers, and bacterial populations recovered. For dressings with hydrophobic base materials, the level of efficacy correlated with the silver species loaded. Biofilm bacteria, which survived the initial silver treatment, were susceptible to tobramycin, ciprofloxacin, and trimethoprim-sulfamethoxazole, in contrast to untreated biofilms, which were highly tolerant to the same antibiotics. This acquired susceptibility was unaffected by the longevity of pretreatment with the silver dressings but depended on the dressing used. The antimicrobial efficacy of the dressings correlated with the type of the dressing base material and silver species loaded.

Kostenko, Victoria; Lyczak, Jeffrey; Turner, Katherine; Martinuzzi, Robert John

2010-01-01

157

Negative-pressure wound dressings to secure split-thickness skin grafts in the perineum.  

PubMed

Several researches have shown that negative-pressure wound dressings can secure split-thickness skin grafts and improve graft survival. However, in anatomically difficult body regions such as the perineum it is questionable whether these dressings have similar beneficial effects. In this study, we evaluated the effects of negative-pressure wound dressings on split-thickness skin grafts in the perineum by comparing wound healing rate and complication rate with that of tie-over dressings. A retrospective chart review was performed for the patients who underwent a split-thickness skin graft to reconstruct perineal skin defects between January 2007 and December 2011. After grafting, the surgeon selected patients to receive either a negative-pressure dressing or a tie-over dressing. In both groups, the initial dressing was left unchanged for 5 days, then changed to conventional wet gauze dressing. Graft success was assessed 2 weeks after surgery by a single clinician. A total of 26 patients were included in this study. The mean age was 56·6 years and the mean wound size was 273·1 cm(2). Among them 14 received negative-pressure dressings and 12 received tie-over dressings. Negative-pressure dressing group had higher graft taken rate (P = 0·036) and took shorter time to complete healing (P = 0·01) than tie-over dressing group. The patients with negative-pressure dressings had a higher rate of graft success and shorter time to complete healing, which has statistical significance. Negative-pressure wound dressing can be a good option for effective management of skin grafts in the perineum. PMID:22958590

Lee, Kyeong-Tae; Pyon, Jai-Kyong; Lim, So-Young; Mun, Goo-Hyun; Oh, Kap Sung; Bang, Sa-Ik

2014-04-01

158

Necrotising fasciitis due to Absidia corymbifera in wounds dressed with non sterile bandages.  

PubMed

We present three cases of Absidia corymbifera necrotising fasciitis presenting to our centre within 1 month of each other. All patients had wound dressings with non sterile crepe bandages at peripheral centres. One patient was lost to follow-up, another improved on timely antifungal therapy, while the last patient succumbed to disseminated infection. We propose that traumatic and deep wounds be dressed with sterile roller bandages to prevent outbreaks of wound zygomycosis. PMID:21883933

Shakoor, Sadia; Jabeen, Kauser; Idrees, Romana; Jamil, Bushra; Irfan, Seema; Zafar, Afia

2011-12-01

159

Multifunctional medicated lyophilised wafer dressing for effective chronic wound healing.  

PubMed

Wafers combining weight ratios of Polyox with carrageenan (75/25) or sodium alginate (50/50) containing streptomycin and diclofenac were prepared to improve chronic wound healing. Gels were freeze-dried using a lyophilisation cycle incorporating an annealing step. Wafers were characterised for morphology, mechanical and in vitro functional (swelling, adhesion, drug release in the presence of simulated wound fluid) characteristics. Both blank (BLK) and drug-loaded (DL) wafers were soft, flexible, elegant in appearance and non-brittle in nature. Annealing helped to improve porous nature of wafers but was affected by the addition of drugs. Mechanical characterisation demonstrated that the wafers were strong enough to withstand normal stresses but also flexible to prevent damage to newly formed skin tissue. Differences in swelling, adhesion and drug release characteristics could be attributed to differences in pore size and sodium sulphate formed because of the salt forms of the two drugs. BLK wafers showed relatively higher swelling and adhesion than DL wafers with the latter showing controlled release of streptomycin and diclofenac. The optimised dressing has the potential to reduce bacterial infection and can also help to reduce swelling and pain associated with injury due to the anti-inflammatory action of diclofenac and help to achieve more rapid wound healing. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci. PMID:24700434

Pawar, Harshavardhan V; Boateng, Joshua S; Ayensu, Isaac; Tetteh, John

2014-06-01

160

A comparative study to evaluate the effect of honey dressing and silver sulfadiazene dressing on wound healing in burn patients  

PubMed Central

To compare the effect of honey dressing and silver-sulfadiazene (SSD) dressing on wound healing in burn patients. Patients (n=78) of both sexes, with age group between 10 and 50 years and with first and second degree of burn of less than 50% of TBSA (Total body surface area) were included in the study, over a period of 2 years (2006-08). After stabilization, patients were randomly attributed into two groups: ‘honey group’ and ‘SSD group’. Time elapsed since burn was recorded. After washing with normal saline, undiluted pure honey was applied over the wounds of patients in the honey group (n=37) and SSD cream over the wounds of patients in SSD group (n=41), everyday. Wound was dressed with sterile gauze, cotton pads and bandaged. Status of the wound was assessed every third and seventh day and on the day of completion of study. Patients were followed up every fortnight till epithelialization. The bacteriological examination of the wound was done every seventh day. The mean age for case (honey group) and control (SSD group) was 34.5 years and 28.5 years, respectively. Wound swab culture was positive in 29 out of 36 patients who came within 8 hours of burn and in all patients who came after 24 hours. The average duration of healing in patients treated with honey and SSD dressing at any time of admission was 18.16 and 32.68 days, respectively. Wound of all those patients (100%) who reported within 1 hour became sterile with honey dressing in less than 7 days while none with SSD. All of the wounds became sterile in less than 21 days with honey, while tthis was so in only 36.5% with SSD treated wounds. The honey group included 33 patients reported within 24 hour of injury, and 26 out of them had complete outcome at 2 months of follow-up, while numbers for the SSD group were 32 and 12. Complete outcome for any admission point of time after 2 months was noted in 81% and 37% of patients in the honey group and the SSD group. Honey dressing improves wound healing, makes the wound sterile in lesser time, has a better outcome in terms of prevention of hypertrophic scarring and post-burn contractures, and decreases the need of debridement irrespective of time of admission, when compared to SSD dressing.

Baghel, P. S.; Shukla, S.; Mathur, R. K.; Randa, R.

2009-01-01

161

Efficiency of Microbial Cellulose Dressing in Partial-Thickness Burn Wounds  

PubMed Central

Microbial cellulose is a natural polymer that can hold a quantity of water without any disconformities. Therefore, it is proposed for use as wound dressing material. We report a case of 28% total body surface area partial-thickness flame burn: approximately 4.5% superficial partial-thickness burns on anterior face and 23.5% combined superficial and deep partial-thickness burns on both upper arms and anterior trunk. A microbial cellulose dressing, Nanocell (Thai Nano Cellulose Co Ltd, Bangkok, Thailand), was applied to the face wound only once, without any further dressing change. Progress of healing, until full epithelialization on the face, was observed for 2 weeks. During the treatment period, the patient did not show any irritation or allergic reaction to this new dressing, and wound swab culture showed no evidence of bacteria presence. This innovative material can be an alternative dressing for superficial partial-thickness burn wounds.

Muangman, Pornprom; Opasanon, Supaporn; Suwanchot, Supaparn; Thangthed, Orapin

2011-01-01

162

Alginates from wound dressings activate human macrophages to secrete tumour necrosis factor- ?  

Microsoft Academic Search

Alginates are used to manufacture a number of wound dressings. Clinical observations indicate that they may initiate or accelerate healing of chronic wounds after treatment of underlying pathology. Wound granulation tissue contains large numbers of macrophages and they are thought to regulate the healing process. As purified alginates have been demonstrated to activate macrophages this study was initiated to determine

A Thomas; K. G Harding; K Moore

2000-01-01

163

Protease Inhibition by Oleic Acid Transfer From Chronic Wound Dressings to Albumin  

Microsoft Academic Search

High elastase and cathepsin G activities have been observed in chronic wounds. These levels can inhibit healing through degradation of growth factors, cytokines, and extracellular matrix proteins. Oleic acid (18:1) is a non-toxic elastase inhibitor with some potential for redressing the imbalance of elastase activity found in chronic wounds. Cotton wound dressing material was characterized as a transfer carrier for

J. V. Edwards; Phyllis Howley; Rachel M. Davis; Andrew D. Mashchak; Steven C. Goheen

2007-01-01

164

Controlled induction of distributed microdeformation in wounded tissue via a microchamber array dressing.  

PubMed

Mechanical stimuli are known to play an important role in determining the structure and function of living cells and tissues. Recent studies have highlighted the role of mechanical signals in mammalian dermal wound healing. However, the biological link between mechanical stimulation of wounded tissue and the subsequent cellular response has not been fully determined. The capacity for researchers to study this link is partially limited by the lack of instrumentation capable of applying controlled mechanical stimuli to wounded tissue. The studies outlined here tested the hypothesis that it was possible to control the magnitude of induced wound tissue deformation using a microfabricated dressing composed of an array of open-faced, hexagonally shaped microchambers rendered in a patch of silicone rubber. By connecting the dressing to a single vacuum source, the underlying wounded tissue was drawn up into each of the microchambers, thereby inducing tissue deformation. For these studies, the dressings were applied to full-thickness murine dermal wounds with 200 mmHg vacuum for 12 h. These studies demonstrated that the dressing was capable of inducing wound tissue deformation with values ranging from 11 to 29%. Through statistical analysis, the magnitude of the induced deformation was shown to be a function of both microchamber height and width. These results demonstrated that the dressing was capable of controlling the amount of deformation imparted in the underlying tissue. By allowing the application of mechanical stimulation with varying intensities, such a dressing will enable the performance of sophisticated mechanobiology studies in dermal wound healing. PMID:20607869

Kane, Bartholomew J; Younan, George; Helm, Douglas; Dastouri, Pouya; Prentice-Mott, Harrison; Irimia, Daniel; Chan, Rodney K; Toner, Mehmet; Orgill, Dennis P

2010-11-01

165

The Controlled Induction of Distributed Microdeformation in Wounded Tissue via a Microchamber Array Dressing  

PubMed Central

Mechanical stimuli are known to play an important role in determining the structure and function of living cells and tissues. Recent studies have highlighted the role of mechanical signals in mammalian dermal wound healing. However, the biological link between mechanical stimulation of wounded tissue and the subsequent cellular response has not been fully determined. The capacity for researchers to study this link is partially limited by the lack of instrumentation capable of applying controlled mechanical stimuli to wounded tissue. The studies outlined here tested the hypothesis that it was possible to control the magnitude of induced wound tissue deformation using a microfabricated dressing, comprised of an array of open-faced, hexagonally-shaped microchambers rendered in a patch of silicone rubber. By connecting the dressing to a single vacuum source, the underlying wounded tissue was drawn up into each of the microchambers, thereby inducing tissue deformation. For these studies, the dressings were applied to full thickness murine dermal wounds with 200 mmHg vacuum for 12 hours. The studies demonstrated that the dressing was capable of inducing wound tissue deformation with values ranging from 11 to 29 %. Through statistical analysis, the magnitude of the induced deformation was shown to be a function of both microchamber height and width. These results demonstrated that the dressing was capable of controlling the amount of deformation imparted in the underlying tissue. By allowing the application of mechanical stimulation with varying intensity, such a dressing will enable the performance of sophisticated mechanobiology studies in dermal wound healing.

Kane, Bartholomew J.; Younan, George; Helm, Douglas; Dastouri, Pouya; Prentice-Mott, Harrison; Irimia, Daniel; Chan, Rodney K.; Toner, Mehmet; Orgill, Dennis P.

2010-01-01

166

Novel technique for skin grafting parastomal wounds using a negative-pressure dressing.  

PubMed

Grafting parastomal wounds remains a challenging procedure because of frequent contamination, difficulty isolating the grafted area from the stoma, and an unfavorable environment for skin graft take. The use of negative-pressure dressings has been shown to improve skin graft take by removing excess fluid between the graft and the wound bed, thereby accelerating engraftment. The benefits of negative-pressure dressing around a stoma, however, may be hindered by vacuuming stool into the dressed area. This article reviews a novel approach used for the isolation of a stoma from the parastomal wound area to increase skin graft take in an 82-year-old woman. PMID:24836615

Clavijo-Alvarez, Julio A

2014-06-01

167

The visualisation and speed of kill of wound isolates on a silver alginate dressing.  

PubMed

In chronic wound management, alginate dressings are used to absorb exudate and reduce the microbial burden. Silver alginate offers the added benefit of an additional antimicrobial pressure on contaminating microorganisms. This present study compares the antimicrobial activity of a RESTORE silver alginate dressing with a silver-free control dressing using a combination of in vitro culture and imaging techniques. The wound pathogens examined included Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, ?-haemolytic Streptococcus, and strictly anaerobic bacteria. The antimicrobial efficacy of the dressings was assessed using log(10) reduction and 13-day corrected zone of inhibition (CZOI) time-course assays. Confocal laser scanning microscopy (CLSM) was used to visualise the relative proportions of live/dead microorganisms sequestered into the dressings over 24 hours and estimate the comparative speed of kill. The RESTORE silver alginate dressing showed significantly greater log(10) reductions and CZOIs for all microorganisms compared with the control, indicating the antimicrobial effect of ionic silver. Antimicrobial activity was evident against all test organisms for up to 5 days and, in some cases, up to 12 days following an on-going microbial challenge. Imaging bacteria sequestered in the silver-free dressing showed that each microbial species aggregated in the dressing and remained viable for more than 20 hours. Growth was not observed inside of the dressing, indicating a possible microbiostatic effect of the alginate fibres. In comparison, organisms in the RESTORE silver alginate dressing were seen to lose viability at a considerably greater rate. After 16 hours of contact with the RESTORE silver alginate dressing, >90% of cells of all bacteria and yeast were no longer viable. In conclusion, collectively, the data highlights the rapid speed of kill and antimicrobial suitability of this RESTORE silver alginate dressing on wound isolates and highlights its overwhelming ability to manage a microbial wound bioburden in the management of infected wounds. PMID:22405034

Hooper, Samuel J; Percival, Steven L; Hill, Katja E; Thomas, David W; Hayes, A J; Williams, David W

2012-12-01

168

Economic Evaluation of the Treatment of Chronic Wounds: Hydroactive Wound Dressings in Combination with Enzymatic Ointment Versus Gauze Dressings in Patients with Pressure Ulcer and Venous Leg Ulcer in Germany  

Microsoft Academic Search

Objective: The treatment costs for pressure ulcers and venous leg ulcers were estimated based on the hospital administrator's perspective in Germany. Design: A spreadsheet model using input data from various hospitals in Germany was developed. Interventions: Five currently used treatment strategies were analysed: gauze, impregnated gauze, calcium alginate and hydroactive wound dressing with enzymatic ointment. Participants: All cases used for

Rito Bergemann; Karl W. Lauterbach; Wolfgang Vanscheidt; Klaus-Dieter Neander; Reinhard Engst

1999-01-01

169

The effect of a silver-containing Hydrofiber dressing on superficial wound bed and bacterial balance of chronic wounds.  

PubMed

The treatment of chronic wounds represents a major cost to society and has a profound effect on the participant's quality of life. Chronic wounds may have an increased bacterial burden that can impair healing without all the clinical signs of infection. Silver dressings may provide an alternative topical method to control bacterial burden. The primary aim of this study was to evaluate the clinical improvement in chronic wounds through the effect on wound size, maceration, resolution of surface slough and conversion to healthy granulation during a 4-week application of the silver-containing Hydrofiber dressing. This was a single centre, open-label case series study which included a total of 30 evaluable participants: four with diabetic neuropathic foot ulcers, 13 venous stasis ulcers, four pressure ulcers and nine miscellaneous wounds that did not fit any of the previous categories. All participants had adequate vascular supply, indicating the potential to heal. The wounds were stalled or had the signs and symptoms consistent with critical colonisation. The underlying cause of the ulceration was identified and corrected, or the symptoms and signs were treated. This was followed by the application of silver-containing Hydrofiber dressings for a period of 4 weeks. The majority of wounds treated decreased in size (70%) with decreased exudate, decreased purulence and resolution of surface slough (75%). There was an increased quality and quantity of healthy granulation tissue. Unlike some silver dressings, the Hydrofiber and silver combination dressing was unlikely to cause burning and stinging on application. Peri-wound maceration was present in 54% of participants at baseline, and 85% of these resolved with this dressing. A desloughing action was seen in those patients with pre-existing slough at baseline and its removal will lower the bacterial burden of the wound. PMID:16618321

Coutts, Pat; Sibbald, R Gary

2005-12-01

170

A prospective clinical audit of a new dressing design for lower limb arthroplasty wounds.  

PubMed

An audit of wound healing in patients undergoing total hip or knee arthroplasty in a Scottish hospital found that use of a barrier film made no difference to the effectiveness of a new Hydrofiber/hydrocolloid dressing combination. PMID:19131911

Clarke, J V; Deakin, A H; Dillon, J M; Emmerson, S; Kinninmonth, A W G

2009-01-01

171

Assessment of a Novel In Situ Forming Wound Dressing for Military Use.  

National Technical Information Service (NTIS)

Presented is an evaluation of the biocompatibility and physical properties of a novel hydrogel which cures in situ to form an effective wound dressing. This dressing has the capability of meeting the needs of both the far forward soldier and for use in ho...

A. Lu J. Jackson S. Shums T. Hirt X. Zhai

2008-01-01

172

The Effects of Alginate and Non-Alginate Wound Dressings on Blood Coagulation and Platelet Activation  

Microsoft Academic Search

Alginates are used as haemostats in wound dressings. They act as calcium ion (Ca) donors as they contain mannuronic (M) or guluronic (G) groups with a high Ca content. This study compared the effects of calcium and zinc containing alginates and non-alginate dressings on blood coagulation and platelet activation to determine which was the best haemostat. We showed that alginate

Helen C. Segal; Beverley J. Hunt; K. Gilding

1998-01-01

173

A superabsorbent polymer-containing wound dressing efficiently sequesters MMPs and inhibits collagenase activity in vitro.  

PubMed

Superabsorbent polymer (SAP)-containing wound dressings present a valuable and unique category of wound management products. An in vitro approach was used to assess the effects of a new SAP dressing in treatment of non-healing wounds. It was shown that the SAP dressing possesses a significant binding capacity for MMP-2 and MMP-9 in vitro (P\\0.001). The inclusion of the bound proteases was so strong that no MMP-2 and only marginal amounts of MMP-9 were released from the dressing samples in a subsequent elution step. In addition, the SAP dressing was able to take up collagenase and reduce its activity in vitro. However, collagenase was not completely inactivated upon binding and enzyme-mediated substrate turnover could be observed at the dressings. In conclusion, in vitro data confirm the positive effect of the SAP wound dressing observed in vivo. The findings suggest that it should be specifically useful for highly exuding wounds with an elevated proteolytic activity that needs to be reduced to support healing. PMID:23797827

Wiegand, Cornelia; Hipler, Uta-Christina

2013-10-01

174

Polyelectrolyte complex composed of chitosan and sodium alginate for wound dressing application  

Microsoft Academic Search

Drug-impregnated polyetectrolyte complex (PEC) sponge composed of chitosan and sodium alginate was prepared for wound dressing application. The morphological structure of this wound dressing was observed to be composed of a dense skin outer layer and a porous cross-section layer by scanning electron microscopy (SEM). Equilibrium water content and release of silver sulfadiazine (AgSD) could be controlled by the number

Hyun-Jung Kim; Hyun-Chul Lee; Jong-Suk Oh; Boo-Ahn Shin; Chang-Seok Oh; Ro-Dong Park; Kap-Seung Yang; Chong-Su Cho

1999-01-01

175

Hyaluronic acid and silver sulfadiazine-impregnated polyurethane foams for wound dressing application  

Microsoft Academic Search

Five different kinds of PU foam wound dressings were prepared to investigate their wound healing capability. They include (i) PU+silver sulfadiazine (AgSD), (ii) PU+alginate (Al), (iii) PU+Al+AgSD, (iv) PU+hyaluronic acid (HA), and (v) PU+HA+AgSD. Physical properties and in vitro behaviors of AgSD release and fibroblast adhesion on those dressings were evaluated. From the drug release and fibroblast adhesion studies, it

Y.-S. Cho; J.-W. Lee; J.-S. Lee; T. R. Yoon; Y. Kuroyanagi; M. H. Park; H. J. Kim

2002-01-01

176

Antimicrobial and release study of drug loaded PVA/PEO/CMC wound dressings.  

PubMed

The aim of the present study was to develop PVA/PEO/CMC/aloe vera (PPCAV) and PVA/PEO/CMC/curcumin (PPCCu) dressings with nonwoven polyester fabric as the support layer via freeze-drying (FD) approach. Tetracycline hydrochloride drug (TC) was loaded along with curcumin and aloe vera on these dressings. The morphology of the dressings was characterized by scanning electron microscopy. The swelling behavior, water vapor transmission rate (WVTR), in vitro drug release and antimicrobial nature were analyzed to assess the applicability of these freeze-dried membranes as wound dressing materials. The results show that these dressings made from PPCAVTC and PPCCuTC were highly porous with three-dimensional interconnected porous morphology. The cumulative release of drug from the dressings increases with increasing immersion time and continued up to 24 h, after that it gets leveled off. These dressings evidenced wonderful antimicrobial nature in vitro. These dressings were found to have more than 900 % PBS uptake, WVTR was found to be in the range 2,000-2,500 gm(-2) day(-1). These dressings possess many characteristics desirable in an ideal wound dressing material. PMID:24599552

Gupta, Bhuvanesh; Agarwal, Roopali; Sarwar Alam, M

2014-06-01

177

Topical application of dressing with amino acids improves cutaneous wound healing in aged rats.  

PubMed

The principal goal in treating surgical and non-surgical wounds, in particular for aged skin, is the need for rapid closure of the lesion. Cutaneous wound healing processes involve four phases including an inflammatory response with the induction of pro-inflammatory cytokines. If inflammation develops in response to bacterial infection, it can create a problem for wound closure. Reduced inflammation accelerates wound closure with subsequent increased fibroblast function and collagen synthesis. On the contrary, prolonged chronic inflammation results in very limited wound healing. Using histological and immunohistochemical techniques, we investigated the effects of a new wound dressing called Vulnamin that contains four essential amino acids for collagen and elastin synthesis plus sodium ialuronate (Na-Ial), compared with Na-Ial alone, in closure of experimental cutaneous wounds of aged rats. Our results showed that the application of Vulnamin dressings modulated the inflammatory response with a reduction in the number of inflammatory cells and inducible nitric oxide synthase (iNOS) immunolocalisation, while increasing endothelial nitric oxide synthase (eNOS) and transforming growth factor-beta1 (TGF-beta1) immunolocalisation. Furthermore, the dressing increased the distribution density of fibroblasts and aided the synthesis of thin collagen fibers resulting in a reduction in healing time. The nutritive approach using this new wound dressing can provide an efficacious and safe strategy to accelerate wound healing in elderly subjects, simplifying therapeutic procedures and leading to an improved quality of life. PMID:19560799

Corsetti, Giovanni; D'Antona, Giuseppe; Dioguardi, Francesco Saverio; Rezzani, Rita

2010-09-01

178

Exploratory Development of an Ultra fast-Curing Wound Dressing.  

National Technical Information Service (NTIS)

We are developing a drug-dispensing field dermal dressing. The dermal dressing, which can be easily applied by an untrained person, contains antimicrobials to prevent bacterial infection. The medicated dermal dressing is made of an ultra-fast curing polyu...

K. Dasse D. Dempsey R. Thirucote

1990-01-01

179

Exploratory Development of an Ultra-Fast-Curing Wound Dressing.  

National Technical Information Service (NTIS)

We are developing a drug-dispensing field dermal dressing. The dermal dressing, which can be easily applied by an untrained person, contains antimicrobials to prevent bacterial infection. The medicated dermal dressing is made of an ultra-fast curing polyu...

K. Dasse D. Dempsey R. Thirucote

1989-01-01

180

Exploratory Development of an Ultrafast-Curing Wound Dressing.  

National Technical Information Service (NTIS)

We are developing a drug-dispensing field dermal dressing. The dermal dressing, which can be easily applied by an untrained person, contains a dual antibiotic to prevent bacterial infection. The medicated dermal dressing is made of an ultra-fast-curing po...

M. Szycher D. D. Dempsey

1988-01-01

181

Electrospun nanofibers as dressings for chronic wound care: advances, challenges, and future prospects.  

PubMed

Chronic non-healing wounds show delayed and incomplete healing processes and in turn expose patients to a high risk of infection. Treatment currently focuses on dressings that prevent microbial infiltration and keep a balanced moisture and gas exchange environment. Antibacterial delivery from dressings has existed for some time, with responsive systems now aiming to trigger release only if infection occurs. Simultaneously, approaches that stimulate cell proliferation in the wound and encourage healing have been developed. Interestingly, few dressings appear capable of simultaneously impairing or treating infection and encouraging cell proliferation/wound healing. Electrospinning is a simple, cost-effective, and reproducible process that can utilize both synthetic and natural polymers to address these specific wound challenges. Electrospun meshes provide high-surface area, micro-porosity, and the ability to load drugs or other biomolecules into the fibers. Electrospun materials have been used as scaffolds for tissue engineering for a number of years, but there is surprisingly little literature on the interactions of fibers with bacteria and co-cultures of cells and bacteria. This Review examines the literature and data available on electrospun wound dressings and the research that is required to develop smart multifunctional wound dressings capable of treating infection and healing chronic wounds. PMID:24678050

Abrigo, Martina; McArthur, Sally L; Kingshott, Peter

2014-06-01

182

Evaluation of semi-interpenetrating polymer networks composed of chitosan and poloxamer for wound dressing application.  

PubMed

We have elsewhere reported the work on the preparation of semi-interpenetrating polymer networks (SIPNs) composed of chitosan (CS) and poloxamer to improve the mechanical strength of CS sponge. This study focuses on evaluation of the CS/poloxamer SIPNs to intend for wound dressing application and the efficacy of dehydroepiandrosterone (DHEA)-loaded CS/poloxamer SIPNs in the wound model studies. The properties required for ideal wound dressing, such as equilibrium water content (EWC), water absorption (A(w)), water vapor transmission rate (WVTR), and evaporative water loss, were examined. The CS/poloxamer SIPNs were found to have a water content of 90% of their weight which could prevent the wound bed from accumulation of exudates and also have excellent water adsorption. The WVTR of CS/poloxamer SIPNs was found to be 2,508.2+/-65.7gm(-2)day(-1), indicating that the SIPNs can maintain a moist environment over wound bed in moderate to heavily exuding wound which enhances epithelial cell migration during the healing process. Also, the CS/poloxamer SIPNs in vitro assessment showed proper biodegradation and low cytotoxicity for wound dressing application. The wound healing efficacy of CS/poloxamer SIPNs as a wound dressing was evaluated on experimental full thickness wounds in a mouse model. It was found that the wounds covered with CS/poloxamer SIPNs or DHEA-loaded CS/poloxamer SIPNs were completely filled with new epithelium without any significant adverse reactions after 3 weeks. The results thus indicate that CS/poloxamer SIPNs could be employed in the future as potential wound dressing materials. PMID:17482781

Kim, I Y; Yoo, M K; Seo, J H; Park, S S; Na, H S; Lee, H C; Kim, S K; Cho, C S

2007-08-16

183

Study of wound dressing structure and hydration/dehydration properties  

NASA Astrophysics Data System (ADS)

Hydrogels manufactured by radio-induced crosslinking and simultaneous sterilisation of hydrogels of PVP, PEG and agar, according to the Rosiak method, have many desirable properties for using as wound dressings. However, some properties need to be improved or better controlled. The membranes need to be strong enough to be freely used. Another important property to be controlled is the capacity of absorption of exudate and the kinetics of drying. Therefore, it was necessary to understand the role of main parameters (agar, PVP, PEG concentration and dose) in the structure of the net and in the hydration and dehydration properties. The structure of the membranes was studied by sol analysis and the hydrating/dehydrating properties were studied by isothermal thermogravimetric analysis. The gel content for all samples were always in agreement with expected values considering that only PVP undergoes crosslinking. The hydrating and dehydration results did not show variation with the tested parameters. It was concluded that the network was solely composed of crosslinked PVP plasticezed by the other compounds. The properties of hydration/dehydration is related rather to diffusion than to capillarity or osmose and to the chemical retention of water in the polymeric matrix.

Lugão, A. B.; Machado, L. D. B.; Miranda, L. F.; Alvarez, M. R.; Rosiak, J. M.

1998-06-01

184

Clinical application of new chitin non-woven fabric and new chitin sponge sheet as wound dressing  

Microsoft Academic Search

Two types of new chitin dressings, mesh reinforcement type and sponge-sheet type, were manufactured and applied to skin wounds such as burns and donor sites. The advantages include pain relief, wound hemostasis, adherence and drying. In comparison to the regular type of chitin, the new dressings show no autolysis or breaking and have possibilities for long-term use in wound with

Y. Ohshima; K. Nishino; R. Okuda; A. Minami; K. Kihune

1991-01-01

185

Electrospun zwitterionic poly(sulfobetaine methacrylate) for nonadherent, superabsorbent, and antimicrobial wound dressing applications.  

PubMed

Zwitterionic poly(sulfobetaine methacrylate) (PSBMA) has been well studied for its superhydrophilic and ultralow biofouling properties, making it a promising material for superabsorbent and nonadherent wound dressings. Electrospinning provides multiple desirable features for wound dressings, including high absorptivity due to high surface-area-to-volume ratio, high gas permeation, and conformability to contour of the wound bed. The goal of this work is to develop a fibrous membrane of PSBMA via electrospinning and evaluate its properties related to wound dressing applications. Being superhydrophilic, PSBMA fibers fabricated by a conventional electrospinning method would readily dissolve in water, whereas if cross-linker is added, the formation of hydrogel would prevent electrospinning. A three-step polymerization-electrospinning-photo-cross-linking process was developed in this work to fabricate the cross-linked electrospun PSBMA fibrous membrane. Such electrospun membrane was stable in water and exhibited high water absorption of 353% (w/w), whereas the PSBMA hydrogel only absorbed 81% water. The electrospun membrane showed strong resistance to protein adsorption and cell attachment. Bacterial adhesion studies using Gram negative P. aeruginosa and Gram positive S. epidermidis showed that the PSBMA electrospun membrane was also highly resistant to bacterial adhesion. The Ag(+)-impregnated electrospun PSBMA membrane was shown microbicidal, against both S. epidermidis and P. aeruginosa. Such electrospun PSBMA membrane is ideal for a novel type of nonadherent, superabsorbent, and antimicrobial wound dressing. The superior water absorption aids in fluid removal from highly exudating wounds while keeping the wound hydrated to support healing. Because of the resistance to protein, cell, and bacterial adhesion, the dressing removal will neither cause patients' pain nor disturb the newly formed tissues. The dressing also prevents the attachment of environmental bacteria and offers broad-spectrum antimicrobial activity. It is the first work to develop the water-stable electrospun PSBMA membrane, which has great potential for wound dressing and other applications. PMID:22545647

Lalani, Reza; Liu, Lingyun

2012-06-11

186

Development of novel chitin/nanosilver composite scaffolds for wound dressing applications.  

PubMed

Antibiotic resistance of microorganisms is one of the major problems faced in the field of wound care and management resulting in complications like infection and delayed wound healing. Currently a lot of research is focused on developing newer antimicrobials to treat wounds infected with antibiotic resistant microorganisms. Silver has been used as an antimicrobial agent for a long time in the form of metallic silver and silver sulfadiazine ointments. Recently silver nanoparticles have come up as a potent antimicrobial agent and are finding diverse medical applications ranging from silver based dressings to silver coated medical devices. Chitin is a natural biopolymer with properties like biocompatibility and biodegradability. It is widely used as a scaffold for tissue engineering applications. In this work, we developed and characterized novel chitin/nanosilver composite scaffolds for wound healing applications. The antibacterial, blood clotting and cytotoxicity of the prepared composite scaffolds were also studied. These chitin/nanosilver composite scaffolds were found to be bactericidal against S. aureus and E. coli and good blood clotting ability. These results suggested that these chitin/nanosilver composite scaffolds could be used for wound healing applications. PMID:19802687

Madhumathi, K; Sudheesh Kumar, P T; Abhilash, S; Sreeja, V; Tamura, H; Manzoor, K; Nair, S V; Jayakumar, R

2010-02-01

187

Electron beam crosslinked PEO and PEO/PVA hydrogels for wound dressing  

NASA Astrophysics Data System (ADS)

In order to prepare polyethylene oxide (PEO) hydrogel for wound dressing, different molecular weight PEO and PEO/poly(vinyl alcohol), PVA blend hydrogels were obtained with electron beam irradiation. Gel formation of PEO in aqueous solution was saturated at 40 kGy and the achieved gel fraction was 60-70%. The PEO hydrogel obtained was very fragile, hence PVA was added at 10-30% to give toughness to the PEO hydrogel. The PEO/PVA hydrogel blend showed satisfactory properties for wound dressing. To evaluate the healing effect of PEO/PVA hydrogel blend for dressing, the hydrogel covered a wound formed on the back of marmots. Healing under the wet environment of the hydrogel dressing had some advantages compared with that of gauze dressing which gives a dry environment: (1) the healing rate is faster, (2) easier to change the dressing, i.e. the hydrogel can be peeled off without any damage to the regenerated surface, and (3) no dressing material remains on the wound.

Yoshii, F.; Zhanshan, Y.; Isobe, K.; Shinozaki, K.; Makuuchi, K.

1999-06-01

188

Controlling wound bioburden with a novel silver-containing Hydrofiber dressing.  

PubMed

Clinicians now recognize that both aerobic and anaerobic microorganisms have the ability to degrade or damage host tissue at a wound site through the production of a variety of enzymes and toxins. Silver-containing dressings offer one method for controlling this polymicrobial wound bioburden, and research efforts are currently ongoing to determine their efficacy against aerobic, anaerobic, and antibiotic-resistant microorganisms. The current study aimed to determine the antimicrobial activity of a new silver-containing Hydrofiber dressing (AQUACEL Ag) on both aerobic and anaerobic microorganisms, using the zone-of-inhibition method. This method provides a measure of the ability of the dressing to make available a sufficient concentration of silver to have an antimicrobial effect. To some extent this test mimics the clinical use of the dressing and predicts its microbicidal activity at the wound-dressing interface. The results show that the silver-containing dressing makes silver available at a dressing-agar interface at a concentration that is effective against a broad range of aerobic, anaerobic, and antibiotic-resistant microorganisms. In the context of wound healing, the results showing antimicrobial activity against antibiotic-resistant microorganisms are particularly important, as the control and eradication of these organisms is a major concern within the health care profession. PMID:15225207

Jones, Samantha A; Bowler, Philip G; Walker, Michael; Parsons, David

2004-01-01

189

Hydrofiber dressing and wound repair: review of the literature and new patents.  

PubMed

Effective management of wound healing is a considerable challenge for clinicians. Patients underlying condition, accurate assessment of the wound and exudates, as well as selection of an appropriate dressing is all important factors for success. A variety of dressings are available to the clinician for the management of exudates. Hydrofiber dressings are a relatively new concept, and can be very cost effective because they can be worn for several days at a time. This report will review clinical evidences on the use of Hydrofiber dressing for the management of epithelial lesions, deal with current knowledge on the mechanism of action of this compound towards the epithelial wound healing process, immunological aspects and will also discuss relevant patents. PMID:21352092

Richetta, Antonio G; Cantisani, Carmen; Li, Vincent W; Mattozzi, Carlo; Melis, Luca; De Gado, Federico; Giancristoforo, Simona; Silvestri, Emidio; Calvieri, Stefano

2011-05-01

190

Effects of dressing type on 3D tissue microdeformations during negative pressure wound therapy: a computational study.  

PubMed

Vacuum-assisted closure (VAC) therapy, also referred to as vacuum-assisted closure negative pressure wound therapy (VAC NPWT), delivered to various dermal wounds is believed to influence the formation of granulation tissue via the mechanism of microdeformational signals. In recent years, numerous experimental investigations have been initiated to study the cause-effect relationships between the mechanical signals and the transduction pathways that result in improved granulation response. To accurately quantify the tissue microdeformations during therapy, a new three-dimensional finite element model has been developed and is described in this paper. This model is used to study the effect of dressing type and subatmospheric pressure level on the variations in the microdeformational strain fields in a model dermal wound bed. Three-dimensional geometric models representing typical control volumes of NPWT dressings were generated using micro-CT scanning of VAC GranuFoam, a reticulated open-cell polyurethane foam (ROCF), and a gauze dressing (constructed from USP Class VII gauze). Using a nonlinear hyperfoam constitutive model for the wound bed, simulated tissue microdeformations were generated using the foam and gauze dressing models at equivalent negative pressures. The model results showed that foam produces significantly greater strain than gauze in the tissue model at all pressures and in all metrics (p<0.0001 for all but epsilon(vol) at -50 mm Hg and -100 mm Hg where p<0.05). Specifically, it was demonstrated in this current work that the ROCF dressing produces higher levels of tissue microdeformation than gauze at all levels of subatmospheric pressure. This observation is consistent across all of the strain invariants assessed, i.e., epsilon(vol), epsilon(dist), the minimum and maximum principal strains, and the maximum shear strain. The distribution of the microdeformations and strain appears as a repeating mosaic beneath the foam dressing, whereas the gauze dressings appear to produce an irregular distribution of strains in the wound surface. Strain predictions from the developed computational model results agree well with those predicted from prior two-dimensional experimental and computational studies of foam-based NPWT (Saxena, V., et al., 2004, "Vacuum-assisted closure: Microdeformations of Wounds and Cell Proliferation," Plast. Reconstr. Surg., 114(5), pp. 1086-1096). In conjunction with experimental in vitro and in vivo studies, the developed model can now be extended into more detailed investigations into the mechanobiological underpinnings of VAC NPWT and can help to further develop and optimize this treatment modality for the treatment of challenging patient wounds. PMID:19154071

Wilkes, R; Zhao, Y; Kieswetter, K; Haridas, B

2009-03-01

191

The Evidence Supporting the Use of Honey as a Wound Dressing  

Microsoft Academic Search

Some clinicians are under the impression that there is little or no evidence to support the use of honey as a wound dressing. To allow sound decisions to be made, this seminar article has covered the various reports that have been published on the clinical usage of honey. Positive findings on honey in wound care have been reported from 17

P. C. Molan

2006-01-01

192

Preparation of Silver Nanoparticles Incorporated Electrospun Polyurethane Nano-fibrous Mat for Wound Dressing  

Microsoft Academic Search

Polyurethane foam is currently used as an exudate absorptive wound dressing material. In this study silver (Ag) nanoparticles were incorporated into electrospun polyurethane (PU) nanofiber to enhance the antibacterial as well as wound healing properties. The electrospinning parameters were optimized for PU with and without silver nanoparticles. Silver nanoparticles were synthesized by aqueous and organic methods. The water absorption, antibacterial

Lakshmi R. Lakshman; K. T. Shalumon; Sreeja V. Nair; R. Jayakumar

2010-01-01

193

Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components  

PubMed Central

Chronic wounds typically have excessive levels of matrix metalloproteinases (MMPs) and proinflammatory cytokines that impair healing. Reducing these detrimental proteins may be key to healing chronic wounds. Proprietary protease blends were formulated specifically to degrade excessive amounts of proinflammatory factors that could prevent wound healing. Applications of protease-containing wound dressings to acute and chronic wounds have been observed clinically to resolve inflammation and appear to aid healing. The purpose of this study was to test in vitro a deliberate blend of proteases for the ability to deactivate or activate known proteins associated with inflammation or healing. Purified human target proteins were incubated with test and control solutions and samples removed at various time points. Blinded samples were tested using a novel infrared protein multiplex sandwich-ELISA-type array technique. Many proinflammatory proteins such as MMPs, cytokines and chemokines were susceptible to degradation. Many proteins such as growth factors, cytokines and TIMP1 were resistant to degradation. Not all proinflammatory proteins were deactivated. Family protein structure did not appear to affect susceptibility to degradation or deactivation. These results suggest that specific protease containing wound dressings appear to reduce multiple detrimental components which may disrupt their deleterious effects on the wound bed and microenvironment. By improving the wound microenvironment through the use of definitive proteases, these novel wound dressings may help transition wounds into the subsequent phase of healing.

Parnell, Laura K.S.

2011-01-01

194

Utilization of optical polarization microscopy in the study of sorption characteristics of wound dressing host materials.  

PubMed

Polarization microscopy was used for evaluation of kinetics of diclofenac sorption in three different wound dressing materials. The sorption kinetics can be evaluated by radii change and intensity of the light traveling through the fiber. The most frequently used host materials for drugs in wound dressings are alginate, polyesters such as polyethylene terephthalate, and viscose. We studied sorption of diclofenac as an example drug. Effective, but rather simple in vitro simulation of diclofenac sorption gives insight into the applicability of the mentioned materials for development of wound healing materials. PMID:24559583

Devetak, Miha; Peršin, Zdenka; Stana-Kleinschek, Karin; Maver, Uroš

2014-04-01

195

Which dressing do donor site wounds need?: study protocol for a randomized controlled trial  

PubMed Central

Background Donor site wounds after split-skin grafting are rather 'standard' wounds. At present, lots of dressings and topical agents for donor site wounds are commercially available. This causes large variation in the local care of these wounds, while the optimum 'standard' dressing for local wound care is unclear. This protocol describes a trial in which we investigate the effectiveness of various treatment options for these donor site wounds. Methods A 14-center, six-armed randomized clinical trial is being carried out in the Netherlands. An a-priori power analysis and an anticipated dropout rate of 15% indicates that 50 patients per group are necessary, totaling 300 patients, to be able to detect a 25% quicker mean time to complete wound healing. Randomization has been computerized to ensure allocation concealment. Adult patients who need a split-skin grafting operation for any reason, leaving a donor site wound of at least 10 cm2 are included and receive one of the following dressings: hydrocolloid, alginate, film, hydrofiber, silicone dressing, or paraffin gauze. No combinations of products from other intervention groups in this trial are allowed. Optimum application and changes of these dressings are pursued according to the protocol as supplied by the dressing manufacturers. Primary outcomes are days to complete wound healing and pain (using a Visual Analogue Scale). Secondary outcomes are adverse effects, scarring, patient satisfaction, and costs. Outcome assessors unaware of the treatment allocation will assess whether or not an outcome has occurred. Results will be analyzed according to the intention to treat principle. The first patient was randomized October 1, 2009. Discussion This study will provide comprehensive data on the effectiveness of different treatment options for donor site wounds. The dressing(s) that will prevail in effectiveness, satisfaction and costs will be promoted among clinicians dealing with such patients. Thus, we aim to contribute a well-designed trial, relevant to all clinicians involved in the care for donor site wounds, which will help enhance uniformity and quality of care for these patients. Trial registration http://www.trialregister.nl, NTR1849. Date registered: June 9, 2009

2011-01-01

196

Role of natural polysaccharides in radiation formation of PVA hydrogel wound dressing  

NASA Astrophysics Data System (ADS)

Radiation processed PVA-polysaccharides hydrogels have been observed to be suitable for producing transparent, flexible, mechanically strong, biocompatible, effective and economical hydrogel dressings. The dressings were formed in single stage irradiation process achieving gel formation and sterilization at 25-30 kGy gamma radiation dose. No synthetic plasticizers and additives were used. Different formulations containing poly-vinylalcohol (PVA) and polysaccharides selected from combinations of agar and carrageenan were used to make the dressings. The selected polysaccharides themselves form thermo-reversible gels and degrade on irradiation. Using concentration of polysaccharides as low as 0.5-2% resulted in increase of tensile strength from 45 g/cm 2 to 411 g/cm 2, elongation from 30% to 410% and water uptake from 25% to 157% with respect to PVA gel without polysaccharides. Besides improving mechanical strength, agar contributes more to elongation and carrageenan to mechanical strength of the gel dressing. PVA formulations containing the polysaccharides show significantly different pre-gel viscosities behaviour. Increasing the concentration of agar in the formulation to about 2% converts the sheet gel to paste gel useful for filling wound cavities. The results indicate that pre irradiation network structure of the formulation plays an important role in determining mechanical properties of the irradiated gel dressing. Formulations containing 7-9% PVA, 0.5-1.5% carrageenan and 0.5-1% agar gave highly effective usable hydrogel dressings. Scanning electron micrographs show highly porous structure of the gel. Clinical trials of wound dressing on human patients established safety and efficacy of the dressing. The dressing has been observed to be useful in treating burns, non-healing ulcers of diabetes, leprosy and other external wounds. The dressings are now being marketed in India under different brand names.

Varshney, Lalit

2007-02-01

197

In vitro assessment of water vapour transmission of synthetic wound dressings  

Microsoft Academic Search

The water vapour transmission rate (WVTR) of 14 commercially available synthetic wound dressings (11 hydrocolloids, 2 hydrogels and 1 polyurethane film) was evaluated over 24 and 48 h periods using a modified ASTM standard method (ASTM E96-90). For the 48 h studies a novel microcomputer-controlled apparatus was employed. The dressings exhibited a wide range of WVTRs (76–9360 g m?2d?1 at

P. Wu; A. C. Fisher; P. P. Foo; D. Queen; J. D. S. Gaylor

1995-01-01

198

Chitosan membrane as a wound-healing dressing: characterization and clinical application.  

PubMed

Chitosan prepared from natural biopolymer chitin and cast into membranes has been tested as wound dressing at the skin-graft donor site in patients. Bactigras, a commonly used impregnated tulle gras bandage, served as a control. Chitosan membrane, prepared with a 75% degree of deacetylation and a thickness of 10 microm, was used in nonmesh or mesh form. The progress in wound healing was compared by clinical and histological examination. Itching and pain sensitivity of the wound dressed area was scored with the use of a visual analogue scale. Mesh chitosan membrane in contrast to the nonmesh membrane allowed blood to ooze into the surrounding gauze. After 10 days, the chitosan-dressed area had been healed more promptly as compared with the Bactigras dressed area. Moreover, the chitosan mesh membrane showed a positive effect on the re-epithelialization and the regeneration of the granular layer. The data confirm that chitosan mesh membrane is a potential substitute for human wound dressing. PMID:15116411

Azad, Abul Kalam; Sermsintham, Niwet; Chandrkrachang, Suwalee; Stevens, Willem Frans

2004-05-15

199

Acemannan-containing wound dressing gel reduces radiation-induced skin reactions in C3H mice  

Microsoft Academic Search

To determine (a) whether a wound dressing gel that contains acemannan extracted from aloe leaves affects the severity of radiation-induced acute skin reactions in C3H mice; (b) if so, whether other commercially available gels such as a personal lubricating jelly and a healing ointment have similar effects; and (c) when the wound dressing gel should be applied for maximum effect.

Dianna B. Roberts; Elizabeth L. Travis

1995-01-01

200

WOUND DRESSINGS: RESULTS OF STUDIES OVER 13 YEARS  

Microsoft Academic Search

Many materials were used in and on experimental- ly inflicted wounds in many studies over a 13-year period. No material prevented decay. The individual tree had a greater ef- fect on the wound than the treatments. Some individual trees of a species closed and compartmentalized wounds rapidly and effectively, regardless of treatment, while other trees did not close and compartmentalize

Alex L. Shigo; Walter C. Shortle

1983-01-01

201

Evaluation of various silver-containing dressing on infected excision wound healing study.  

PubMed

Silver-containing dressings have been widely used for controlling wound infection. However, the relationship between different concentrations of silver in dressings and their antimicrobial activities and wound-healing efficacies remains unclear. In the present study, we (in cooperation with Bio-medical Carbon Technology) investigated various silver-containing activated carbon fibers to understand the effects of different silver concentrations on the efficacies of a silver containing dressing. Our results indicated that various silver-containing activated carbon fibers exhibited good antibacterial effects and biocompatibility in terms of cell viability and that silver concentration showed a minor influence on cell growth. The infected excision wound model indicated that compared to silver-containing activated carbon fiber and other commercial silver-containing dressings assisted wound healing by promoting granulation and collagen deposition. Meanwhile, the silver ion can only be restrained in epidermis by intact skin. During application on the wound area, a temporary increase of serum silver can be detected, but this elevated serum silver level decreased to a subtle level after the removal of silver-containing activated carbon fiber. PMID:24449026

Lin, Yu-Hsin; Hsu, Wei-Shan; Chung, Wan-Yu; Ko, Tse-Hao; Lin, Jui-Hsiang

2014-05-01

202

Antimicrobial properties of silver-containing wound dressings: a microcalorimetric study.  

PubMed

The studies reported here have been undertaken to assess the potential use of isothermal microcalorimetry in studying the antimicrobial efficacy of wound dressings that contain antimicrobial agents. The microcalorimetric technique allows non-invasive and non-destructive analysis to be performed directly on a test sample, regardless of whether it is homogeneous or heterogeneous in nature. Microcalorimetry is an established procedure that offers quantitative measurements and has the distinct advantage over traditional antimicrobial test methodologies in that calorimetric measurements are made continuously over real-time, thus the dynamic response of microorganisms to an antimicrobial agent is observed in situ. The results described in this paper are for interaction of two silver-containing wound care products AQUACEL Ag Hydrofiber (ConvaTec, Deeside, UK) and Acticoat 7 with SILCRYST (Smith and Nephew Healthcare, UK) with the wound pathogenic organisms Staphylococcus aureus and Pseudomonas aeruginosa. Both dressings are shown, microcalorimetrically, to have the capacity to kill these common wound pathogens within 1-2 h of contact. A dose-response study was conducted with the AQUACEL Ag dressing. Microcalorimetry is shown to be rapid, simple and effective in the study of the antimicrobial properties of gel forming wound dressings. PMID:12954181

O'Neill, Michael A A; Vine, George J; Beezer, Anthony E; Bishop, Alistair H; Hadgraft, Jonathan; Labetoulle, Chloé; Walker, Michael; Bowler, Phillip G

2003-09-16

203

Mechanical properties and water vapour permeability of film from Haruan (Channa striatus) and fusidic acid spray for wound dressing and wound healing.  

PubMed

Aerosol is a new dosage form for wound dressing and wound healing. Concentrate of aerosols which were prepared for wound dressing and wound healing will produced films after sprayed onto the surface of wounds. The aim of this study is to evaluate the mechanical and water vapour permeability properties of the films from the aerosol concentrates. Film forming dispersions contained Haruan extract and Fusidic acid as the active ingredients, hydroxypropyl methylcellulose (HPMC) as polymer and polyethylene glycol (PEG) 400, glycerin and propylene glycol as plasticizers. Haruan extract is used to promote healing and Fusidic acid is added in formula as antibiotic to prevent the infections. The films were prepared by using casting technique. Based on the results, it is concluded that films produced from Formula E1, E2 and F4 possessed good elongation at break but low tensile strength. All Formula E, Formula F4 and F5 were permeable but Formula F5 was brittle and would peel off by themselves from the Petri dish. PMID:20363692

Febriyenti; Noor, Azmin Mohd; Bai, Saringat Bin

2010-04-01

204

An in vitro test of the efficacy of silver-containing wound dressings against Staphylococcus aureus and Pseudomonas aeruginosa in simulated wound fluid.  

PubMed

An isothermal microcalorimetric assay was used to quantify the efficacy of a silver-containing wound dressing against two common wound pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. The growth patterns of the two species were unique and varied depending on the environment in which the organisms were grown. Addition of non-silver-containing dressing altered the growth kinetics while addition of silver (contained either in a dressing or as AgNO3 solution) was seen to elicit inhibition and/or kill depending on concentration. Tests were conducted in nutrient broth and simulated wound fluid. It was found that minimum inhibitory and minimum bactericidal concentration values were higher in simulated wound fluid and under anaerobic conditions. Bioavailability of silver from the wound dressing was 35% against S. aureus in nutrient broth and 68% against both species in simulated wound fluid. The data highlight the importance of developing and conducting in vitro assays in biorelevant media. PMID:24374221

Said, Jawal; Dodoo, Cornelius C; Walker, Michael; Parsons, David; Stapleton, Paul; Beezer, Anthony E; Gaisford, Simon

2014-02-28

205

Superabsorbent polymer-containing wound dressings have a beneficial effect on wound healing by reducing PMN elastase concentration and inhibiting microbial growth.  

PubMed

A comprehensive in vitro approach was used to assess the effects of superabsorbent polymer (SAP) containing wound dressings in treatment of non-healing wounds. A slight negative effect on HaCaT cells was noted in vitro which is most likely due to the Ca(2+) deprivation of the medium by binding to the SAP. It could be shown that SAP wound dressings are able to bind considerable amounts of elastase reducing enzyme activity significantly. Furthermore, SAP's inhibit the formation of free radicals. The SAP-containing wound dressings tested also exhibited a significant to strong antimicrobial activity effectively impeding the growth of gram-negative and gram-positive bacteria as well as yeasts. In conclusion, in vitro data confirm the positive effect of SAP wound dressings observed in vivo and suggest that they should be specifically useful for wound cleansing. PMID:21870081

Wiegand, C; Abel, M; Ruth, P; Hipler, U C

2011-11-01

206

Visualisation of bacterial sequestration and bactericidal activity within hydrating Hydrofiber wound dressings.  

PubMed

The fluid handling and microbiological properties of a non-antimicrobial Hydrofiber(NAH) wound dressing have been compared with those of a silver salt-containing Hydrofiber (SCH). Fluorescent dyes (BacLight, Live/Dead Kit) were added to fresh cultures of two wound pathogens (Pseudomonas aeruginosa and Staphylococcus aureus), and used to visualise their viability. Live bacteria stained green and dead/dying bacteria turned red. When inoculated into samples of the NAH and SCH dressings, the viability of the bacteria could be effectively monitored over time using a rapid form of confocal laser scanning microscopy (RCLSM--Leica UK). When the NAH dressing was hydrated with stained bacterial culture, its fibres swelled quickly, reducing interstitial spaces between the fibres, resulting in the formation of a cohesive gel. Bacteria became immobilised in the gel, forming characteristic clumps, but remained largely green (viable) for more than 20 h with no apparent increase in numbers. The SCH initially behaved in a similar manner, however, using 3-D data from RCLSM time-lapse sequences P. aeruginosa was observed to turn progressively red (i.e. died) within 1.5-3 h and S. aureus similarly turned red within 5-7 h of contact with the SCH dressing. The ability of both Hydrofiber dressings to sequester and immobilise potentially pathogenic wound micro-organisms has been demonstrated. Additionally the SCH dressing was shown to kill immobilised bacteria, as a consequence of the ionic silver bactericide. These properties of the Hydrofiber dressings may contribute to providing an environment that is supportive to wound healing. PMID:16120458

Newman, Geoffrey R; Walker, Michael; Hobot, Jan A; Bowler, Philip G

2006-03-01

207

In Vitro Evaluation of the Antimicrobial Effectiveness and Moisture Binding Properties of Wound Dressings  

PubMed Central

A variety of silver-coated dressings and some impregnated with other chemicals are now available in the market; however, there have been few studies analyzing their comparative efficacies as antimicrobial agents. Moreover, their properties for retaining an appropriate level of moisture that is critical for effective wound healing have never been reported. Five commercially available silver-containing and chlorhexidine dressings, Urgotul SSD®, Bactigras®, Acticoat®, Askina Calgitrol Ag® and Aquacel Ag®, were tested to determine their comparative antimicrobial effectiveness in vitro against five common wound pathogens, namely methicillin-sensitive and -resistant Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. Mepitel®, a flexible polyamide net coated with soft silicone, was used as a control. The zones of inhibition and both the rapidity and the extent of killing of these pathogens were evaluated. All five antimicrobial dressings investigated exerted some bactericidal activity, particularly against E. coli. The spectrum and rapidity of action ranged widely for the different dressings. Acticoat® had a broad spectrum of action against both Gram-positive and -negative bacteria. Other dressings demonstrated a narrower range of bactericidal activities. Regarding the absorption and release of moisture, Askina Calgitrol Ag® absorbed and released the most moisture from the environment. Aquacel Ag® also exhibited good moisture absorption and moisture release, but to a lower degree. The other tested dressings absorbed or released very little moisture. Askina Calgitrol Ag® and Aquacel Ag® are good alternative dressings for treating wounds with high exudates and pus. An understanding of the characteristics of these dressings will be useful for utilizing them for specific requirements under specified conditions.

Aramwit, Pornanong; Muangman, Pornprom; Namviriyachote, Nantaporn; Srichana, Teerapol

2010-01-01

208

Moist dressing coverage supports proliferation and migration of transplanted skin micrografts in full-thickness porcine wounds.  

PubMed

Transplantation of skin micrografts in a 1:100 ratio regenerate the epidermis of full-thickness wounds in pigs within 14 days in a wet environment. The aim of the current study was to combine micrografts and commercially available moist dressings. We hypothesized that micrografts regenerate the epidermis when covered with a moist dressing. 5cm×5cm and 10cm×10cm full-thickness wounds were created on the backs of pigs. Wounds were transplanted with 0.8mm×0.8mm micrografts created from a split-thickness skin graft in a 1:100 ratio. 5cm×5cm wounds were treated with wound chambers, moist dressings or dry gauze (non-transplanted control group). 10cm×10cm wounds were compared to non-transplanted wounds, both covered with moist dressings. Reepithelialization was assessed in biopsies from day 10, 14 and 18 post-transplantation. 5cm×5cm transplanted wounds covered with moist dressings showed 69.5±20.6% reepithelialization by day 14 and 90.5±10.4% by day 18, similar to wounds covered with a wound chamber (63.9±16.7 and 86.2±11.9%, respectively). 18 days post-transplantation, 10cm×10cm transplanted wounds covered with moist dressings showed 66.1±10.3% reepithelialization, whereas nontransplanted wounds covered with moist dressings were 40.6±6.6% reepithelialized. We conclude that micrografts combined with clinically available moist dressings regenerate the epidermis of full-thickness wounds. PMID:23838078

Hackl, Florian; Kiwanuka, Elizabeth; Philip, Justin; Gerner, Philipp; Aflaki, Pejman; Diaz-Siso, J Rodrigo; Sisk, Geoffroy; Caterson, E J; Junker, Johan P E; Eriksson, Elof

2014-03-01

209

Antimicrobial activity of silver-containing dressings on wound microorganisms using an in vitro biofilm model.  

PubMed

Antimicrobial dressings such as those containing silver are now being used widely to control wound bioburden, and tests to demonstrate their efficacy predominantly involve in vitro models using free-living or planktonic bacteria. In this present study a wide range of antibiotic-sensitive and resistant bacteria were tested in their quasi-sessile state using a standard agar assay and a second method used a poloxamer gel (true biofilm state - poloxamer encourages microorganisms to exhibit a more clinically relevant biofilm phenotype) technique. The antimicrobial activity of two silver dressings, a silver-containing Hydrofiber (SCH) dressing and a nanocrystalline silver-containing dressing (NCS), were evaluated on a variety of microorganisms, using a zone-of-inhibition (ZOI) test. When grown on agar (presenting a quasi-sessile state of each organism), the antibiotic-susceptible microorganisms were generally more susceptible to the SCH dressing compared with the NCS. ZOIs associated with SCH dressing ranged between 5.7 and 17.5 mm; those for the NCS against the same group of organisms ranged between 1.9 and 8.6 mm. When grown on poloxamer gel, (presenting the biofilm state of each organism) the same group of microorganisms were less susceptible to both dressings. The SCH dressing was most effective against strains of Pseudomonas aeruginosa, Candida albicans and Staphylococcus aureus (ZOI range: 2.6-6 mm); the NCS was most effective against strains of Klebsiella pneumoniae, Enterococcus faecalis and Escherichia coli (i.e. ZOI range: 1-2.8 mm). Similarly to the antibiotic-susceptible microorganisms, nine of ten antibiotic-resistant bacterial strains when grown on agar were more susceptible to the SCH dressing compared with the NCS. Although the microorganisms tested were universally less susceptible to the silver dressings when in their biofilm state, in the majority of cases, the SCH dressing demonstrated greater biofilm-inhibiting activity than the NCS. PMID:17651233

Percival, Steven L; Bowler, Phillip G; Dolman, Jayne

2007-06-01

210

Superabsorbent polymer-containing wound dressings have a beneficial effect on wound healing by reducing PMN elastase concentration and inhibiting microbial growth  

Microsoft Academic Search

A comprehensive in vitro approach was used to assess the effects of superabsorbent polymer (SAP) containing wound dressings\\u000a in treatment of non-healing wounds. A slight negative effect on HaCaT cells was noted in vitro which is most likely due to\\u000a the Ca2+ deprivation of the medium by binding to the SAP. It could be shown that SAP wound dressings are

C. Wiegand; M. Abel; P. Ruth; U. C. Hipler

211

Development of a Wound Dressing Composed of Hyaluronic Acid Sponge Containing Arginine and Epidermal Growth Factor  

Microsoft Academic Search

Hyaluronic acid (HA) has the ability to promote wound healing. Epidermal growth factor (EGF) is able to promote the proliferation of various cell types, in addition to epidermal cells. A novel wound dressing was designed using high-molecular-weight hyaluronic acid (HMW-HA) and low-molecular-weight hyaluronic acid (LMW-HA). Spongy sheets composed of cross-linked high-molecular-weight hyaluronic acid (c-HMW-HA) were prepared by freeze-drying an aqueous

Yasuhiro Matsumoto; Yoshimitsu Kuroyanagi

2010-01-01

212

Hydrogel blends of chitin\\/chitosan, fucoidan and alginate as healing-impaired wound dressings  

Microsoft Academic Search

In order to create a moist environment for rapid wound healing, a hydrogel sheet composed of a blended powder of alginate, chitin\\/chitosan and fucoidan (ACF-HS; 60:20:2:4 w\\/w) has been developed as a functional wound dressing. ACF-HS gradually absorbed DMEM without any maceration, and fluid absorption became constant within 18h. On application, ACF-HS was expected to effectively interact with and protect

Kaoru Murakami; Hiroshi Aoki; Shingo Nakamura; Shin-ichiro Nakamura; Megumi Takikawa; Motoaki Hanzawa; Satoko Kishimoto; Hidemi Hattori; Yoshihiro Tanaka; Tomoharu Kiyosawa; Yasunori Sato; Masayuki Ishihara

2010-01-01

213

Visualisation of bacterial sequestration and bactericidal activity within hydrating Hydrofiber ® wound dressings  

Microsoft Academic Search

The fluid handling and microbiological properties of a non-antimicrobial Hydrofiber® (NAH) wound dressing have been compared with those of a silver salt-containing Hydrofiber® (SCH). Fluorescent dyes (BacLight™, Live\\/Dead™ Kit) were added to fresh cultures of two wound pathogens (Pseudomonas aeruginosa and Staphylococcus aureus), and used to visualise their viability. Live bacteria stained green and dead\\/dying bacteria turned red. When inoculated

Geoffrey R. Newman; Michael Walker; Jan A. Hobot; Philip G. Bowler

2006-01-01

214

Efficient surface functionalization of wound dressings by a phytoactive nanocoating refractory to Candida albicans biofilm development.  

PubMed

The present study reports the fabrication and characterization of a novel nanostructured phyto-bioactive coated rayon/polyester wound dressing (WD) surface refractory to Candida albicans adhesion, colonization and biofilm formation, based on functionalized magnetite nanoparticles and Anethum graveolens (AG) and Salvia officinalis (SO) essential oils (EOs). TEM, XRD, TGA, FT-IR were used for the characterization of the fabricated nanobiocoated WDs. Using magnetic nanoparticles for the stabilization and controlled release of EOs, the activity of natural volatile compounds is significantly enhanced and their effect is stable during time. For this reason the nanobiocoated surfaces exhibited a longer term anti-biofilm effect, maintained for at least 72 h. Besides their excellent anti- adherence properties, the proposed solutions exhibit the advantage of using vegetal natural compounds, which are less toxic and easily biodegradable in comparison with synthetic antifungal drugs, representing thus promising approaches for the development of successful ways to control and prevent fungal biofilms associated infections. PMID:24706124

Anghel, Ion; Holban, Alina Maria; Andronescu, Ecaterina; Grumezescu, Alexandru Mihai; Chifiriuc, Mariana Carmen

2013-12-01

215

In Vivo and Cytotoxic Assays of a Poly(vinyl alcohol)\\/Clay Nanocomposite Hydrogel Wound Dressing  

Microsoft Academic Search

In the present work, a nanocomposite hydrogel wound dressing was prepared on the basis of poly(vinyl alcohol) using organically-modified montmorillonite as nanoclay by the freezing–thawing cyclic method. In vivo assays were performed to evaluate its performance as an applicable wound dressing on animals. It showed an improved healing process for wounds covered by the prepared nanocomposite hydrogel compared with control

Mohammad Sirousazar; Mehrdad Kokabi; Zuhair Muhammad Hassan

2011-01-01

216

Irradiation of polyvinyl alcohol and polyvinyl pyrrolidone blended hydrogel for wound dressing  

Microsoft Academic Search

Polyvinyl alcohol and polyvinyl pyrrolidone (PVA–PVP) blended hydrogel for wound dressing has been prepared by using gamma rays irradiation technique. The gel fraction, mechanical properties, the water content and water absorption performance of the hydrogels were measured. It was found that the gel fraction increases with increasing irradiation dose but never reaches 100% of gel. The PVA\\/PVP blended hydrogel has

Mirzan T Razzak; Darmawan Darwis; Zainuddin; Sukirno

2001-01-01

217

Application of benzyl hyaluronate membranes as potential wound dressings: evaluation of water vapour and gas permeabilities  

Microsoft Academic Search

Membranes of 75% and 100% benzyl hyaluronate esters (percentage of total carboxylate groups esterified) were prepared and their water vapour, oxygen and carbon dioxide transmission rates determined. The values of these properties were compared with the values obtained for several commercial wound dressings under the same conditions. The benzyl hyaluronate membranes showed water vapour transmission rates (2157–2327 g m?2 per

Lisa Ruiz-Cardona; Yeshwant D. Sanzgiri; L. M. Benedetti; Valentino J. Stella; Elizabeth M. Topp

1996-01-01

218

Gamma Radiation?Induced Crosslinked PVA\\/Chitosan Blends for Wound Dressing  

Microsoft Academic Search

Crosslinked polyvinyl alcohol (PVA) and chitosan polymer blends have been prepared by using gamma irradiation. Chitosan was used in the blends to prevent microbiological growth, such as bacteria and fungi on the polymer. The physical properties of the blend, such as gelation, water absorption, and mechanical properties were examined to evaluate the possibility of its application for wound dressing. A

Kariman M. El Salmawi

2007-01-01

219

Novel soy protein wound dressings with controlled antibiotic release: Mechanical and physical properties  

Microsoft Academic Search

Naturally derived materials are becoming widely used in the biomedical field. Soy protein has advantages over various types of natural proteins employed for biomedical applications due to its low price, non-animal origin and relatively long storage time and stability. In the current study soy protein isolate (SPI) was investigated as a matrix for wound dressing applications. The antibiotic drug gentamicin

Zachi Peles; Meital Zilberman

220

The Effect of a New Tissue-Adhesive Wound Dressing on the Healing of Traumatic Abrasions  

Microsoft Academic Search

Background: Octylcyanoacrylate is a new medical-grade adhesive with antimicrobial properties. It forms a thin, flexible, occlusive bandage. Objective: To determine the gross and histological effects of the spray bandage when treating abrasions. Methods: Abrasions were produced on the flanks of 18 albino guinea pigs and randomized to treatment with a control (Biobrane) or octylcyanoacrylate dressing. The wounds were assessed with

James Quinn; Laurie Lowe; Michelle Mertz

2000-01-01

221

In situ production of a synthetic barrier dressing for burn wounds in rats.  

PubMed Central

We describe the in situ production of a burn wound dressing applied to eschar that completely isolates burned tissue from contamination with Pseudomonas aeruginosa. Anesthetized, adult rats were subjected to a scald burn over 10% of their body surfaces. One-half hour later a test dressing presumed to be a barrier to bacterial contamination of the wound was applied to the burned surface. Tannic acid, vaseline, ethyl linoleate, collodion, and polyhydroxethylmethacrylate (PHEMA) were evaluated. Each agent was applied directly to the burned surface. A solid film of the PHEMA was produced on the eschar by addition of solvent and a powdered form of the polymer. The surface of each synthetic dressing was contaminated 30 min after application by the addition of 10(8) P. aeruginosa. Also, a control set of rats was burned and their eschars were directly contaminated without application of the test dressing. Seven days later the contaminated muscle under the burned area in 10 control rats had P. aeruginosa counts of 10(7) to 10(8) per g of muscle (wet weight). Of the materials tested, only PHEMA consistently acted as an effective barrier dressing, reducing bacteria in the muscle to undetectable levels in 11 of 14 tests. It was also possible to treat contaminated eschar through this synthetic dressing by topical application of antibiotics to the barrier surface. The results suggest a novel clinical approach in which a barrier dressing could be used to isolate a burn eschar from environmental and subject contamination until the wound site is ready for grafting.

Nathan, P; MacMillan, B G; Holder, I A

1975-01-01

222

In situ production of a synthetic barrier dressing for burn wounds in rats.  

PubMed

We describe the in situ production of a burn wound dressing applied to eschar that completely isolates burned tissue from contamination with Pseudomonas aeruginosa. Anesthetized, adult rats were subjected to a scald burn over 10% of their body surfaces. One-half hour later a test dressing presumed to be a barrier to bacterial contamination of the wound was applied to the burned surface. Tannic acid, vaseline, ethyl linoleate, collodion, and polyhydroxethylmethacrylate (PHEMA) were evaluated. Each agent was applied directly to the burned surface. A solid film of the PHEMA was produced on the eschar by addition of solvent and a powdered form of the polymer. The surface of each synthetic dressing was contaminated 30 min after application by the addition of 10(8) P. aeruginosa. Also, a control set of rats was burned and their eschars were directly contaminated without application of the test dressing. Seven days later the contaminated muscle under the burned area in 10 control rats had P. aeruginosa counts of 10(7) to 10(8) per g of muscle (wet weight). Of the materials tested, only PHEMA consistently acted as an effective barrier dressing, reducing bacteria in the muscle to undetectable levels in 11 of 14 tests. It was also possible to treat contaminated eschar through this synthetic dressing by topical application of antibiotics to the barrier surface. The results suggest a novel clinical approach in which a barrier dressing could be used to isolate a burn eschar from environmental and subject contamination until the wound site is ready for grafting. PMID:808499

Nathan, P; MacMillan, B G; Holder, I A

1975-08-01

223

Negative pressure wound dressing of the radial forearm donor site  

Microsoft Academic Search

Donor site complications of the radial forearm are a significant cause of post-operative morbidity. 15 patients had radial forearm free tissue donor sites treated with split skin grafts and a negative pressure dressing. All grafts showed 100% take at 5 days. The advantages of this technique include rapid healing at an unfavourable graft recipient site, increased graft take and decreased

C. Avery; J. Pereira; A. Moody; M. Gargiulo; I. Whitworth

2000-01-01

224

Preservation of the properties of elastic plasma protein plus platelet film for wound dressing.  

PubMed

Experimental results have previously been reported for a new biological dressing consisting of concentrated plasma proteins and platelet-rich plasma (PRP), which is named Platelet-Protein film (PPF). Based on the results of this experimental study, a clinical trial was begun to examine the usefulness of PPF. Although fresh autologous PPF is being used in the clinical trial, it is considered that the use of a preserved material prepared in advance would enable more convenient application. To verify the usefulness of preserved PPF, this preliminary study was conducted to examine the effects of fresh PPF, preserved PPF or blood clot, as control, applied to excisional skin defects in healing-impaired mice. The wound area and vascular density were analysed on day 9 after wound creation. Significant decrease of the wound size was observed in the preserved PPF and fresh PPF groups (4.2 (3.7), 2.2 (0.53)%) in comparison with that in the control group (38.5 (18.6)%, p < 0.05). Furthermore, a significant increase of the vascular density was also observed in the preserved PPF and fresh PPF groups (0.055 (0.021), 0.050 (0.019) mm(2)/mm(2)) as compared with that in the control group (0.016 (0.010) mm(2), p < 0.01). There was no significant difference in the effect on the wound size or vascular density between the preserved PPF and fresh PPF groups. The results showed that the properties of PPFs could be maintained for at least 1 week under appropriate storage conditions. The possibility of preservation of PPF for future use could be of practical advantage in actual clinical situations. PMID:23848423

Sano, Hitomi; Ichioka, Shigeru; Minamimura, Ai; Tanaka, Ryuhei; Ikebuchi, Kenji; Suzuki, Miho

2013-12-01

225

Electrospinning of multicomponent ultrathin fibrous nonwovens for semi-occlusive wound dressings.  

PubMed

This work describes the design and assembly of multifunctional and cost-efficient composite fiber nonwovens as semi-occlusive wound dressings using a simple electrospinning process to incorporate a variety of functional components into an ultrathin fiber. These components include non-hydrophilic poly(L-lactide) (PLLA) as fibrous backbone, hydrophilic poly(vinyl pyrrolidone)-iodine (PVP-I), TiO(2) nanoparticles, zinc chloride as antimicrobial, odor-controlling, and antiphlogistic agents, respectively. The process of synthesis starts with a multicomponent solution of PLLA, PVP, TiO(2) nanoparticles plus zinc chloride, in which TiO(2) nanoparticles are synthesized by in situ hydrolysis of TiO(2) precursors in a PVP solution for the sake of obtaining the particle-uniformly dispersive solution. Subsequent electrospinning generates the corresponding composite fibers. A further iodine vapor treatment to the composite fibers combines iodine with PVP to produce the PVP-I complexes. Experiments indicate that the assembled composite fibers (300-400 nm) possess the ointment-releasing characteristic and the phase-separate, core-sheath structures in which PVP-I residing in fiber surface layer becomes the sheath, and PLLA distributing inside the fiber acts as the core. Based on this design, the structural advantages combining active components endow the assembled composite nonwovens with a variety of functions, especially, the existence of PVP-I, endows the nonwoven with water absorbability, antimicrobial activity, adhesive ability, and transformable characteristic from hydrophilicity to non-hydrophilicity. The multifunctional, cost-efficient, and ointment-releasing characteristics make the multicomponent composite fibrous nonwovens potentially useful in applications such as initial stage of dressing of the cankerous or contaminated wounds. PMID:18431786

Hong, Youliang; Li, Yanan; Zhuang, Xiuli; Chen, Xuesi; Jing, Xiabin

2009-05-01

226

Silver nanoparticle/bacterial cellulose gel membranes for antibacterial wound dressing: investigation in vitro and in vivo.  

PubMed

Bacterial cellulose (BC) has attracted increasing attention as a novel wound dressing material, but its antimicrobial activity, which is one of the critical skin-barrier functions in wound healing, is not sufficient for use in practical applications. To overcome such a deficiency, silver nanoparticles were generated and self-assembled on the surface of BC nanofibers, forming a stable and evenly distributed Ag nanoparticle coated BC nanofiber (AgNP-BC). The performance of AgNP-BC was systematically studied in terms of antibacterial activities, cytocompatibility and effects on wound healing. The results showed that AgNP-BC exhibited significant antibacterial activity against Staphylococcus aureus. Moreover, AgNP-BC allowed attachment, and growth of rat fibroblasts with low cytotoxicity emerged. Based on these advantages, AgNP-BC samples were applied in a second-degree rat wound model. Wound flora showed a significant reduction during the healing. The fresh epidermal and dermis thicknesses with AgNP-BC samples were 111 and 855 µm respectively, higher than 74 and 619 µm for BC groups and 57 and 473 µm for untreated control wounds. The results demonstrated that AgNP-BC could reduce inflammation and promote scald wound healing. PMID:24739469

Wu, Jian; Zheng, Yudong; Wen, Xiaoxiao; Lin, Qinghua; Chen, Xiaohua; Wu, Zhigu

2014-06-01

227

Preparation of chitosan/silk fibroin blending membrane fixed with alginate dialdehyde for wound dressing.  

PubMed

The objective of this work was to prepare chitosan/silk fibroin (CS/SF) blending membranes crosslinked with alginate dialdehyde (ADA) as wound dressings and to evaluate the physical properties and biocompatibility of the membranes. The morphology of membrane was observed by scanning electron microscopy (SEM) which showed that the well consistency of these two compositions. Further, the stability, water absorption and water vapor permeability of the ADA fixed CS/SF membranes could meet the needs of wound dressing. Furthermore, the biocompatibility of ADA fixed membranes was investigated by MTT assays and SEM in vitro, and the membranes were found to promote the cell attachment and proliferation. These results suggest that ADA fixed CS/SF blending membranes with a suitable ratio could be a promising candidate for wound healing applications. PMID:23562962

Gu, Zhipeng; Xie, HuiXu; Huang, Chengcheng; Li, Li; Yu, Xixun

2013-07-01

228

Water vapour transmission rates in burns and chronic leg ulcers: influence of wound dressings and comparison with in vitro evaluation.  

PubMed

One of the main functions of wound dressings is to control water vapour transmission rate (WVTR) from wounded skin. In this paper, the influence of hydrocolloid, knitted viscose and gauze dressings was evaluated through in vivo measurement of WVTR in burns and chronic leg ulcers utilizing an evaporimeter. The results suggest that the evaporative water vapour loss from exposed skin wounds depends mainly on the wound depth, and that chronic leg ulcers have the same level of the WVTR as full thickness burns. Compared with the knitted viscose and gauze dressings, hydrocolloid dressing has a greater effect on reducing evaporative water loss, with WVTR being 20-30% of that of exposed wounds under the conditions used in this study. This result is in agreement with that obtained in an in vitro evaluation. PMID:8830962

Wu, P; Nelson, E A; Reid, W H; Ruckley, C V; Gaylor, J D

1996-07-01

229

In situ forming and rutin-releasing chitosan hydrogels as injectable dressings for dermal wound healing.  

PubMed

An in situ gel-forming system composed of rutin- and tyramine-conjugated chitosan derivatives, horseradish peroxidase (HRP), and hydrogen peroxide (H(2)O(2)) was prepared and applied to dermal wound repair. Rutin was employed to enhance production and accumulation of extracellular matrix in the healing process. In vitro study demonstrates that released rutin significantly enhanced cell proliferation as compared with media without rutin. In vivo wound healing study was performed by injecting hydrogels on rat dorsal wounds with a diameter of 8 mm for 14 days. Histological results demonstrated that rutin-conjugated hydrogel exhibited enhancement of wound healing as compared with treatments with PBS, hydrogel without rutin, and a commercialized wound dressing (Duoderm). More specifically, rutin-conjugated hydrogels induced better defined formation of neo-epithelium and thicker granulation, which is closer to the original epithelial tissue. As a result, this study suggests that the in situ gel-forming system can be a promising injectable gel-type wound dressing. PMID:21591793

Tran, Ngoc Quyen; Joung, Yoon Ki; Lih, Eugene; Park, Ki Dong

2011-08-01

230

In vivo evaluation of chitosan-PVP-titanium dioxide nanocomposite as wound dressing material.  

PubMed

In our present study, the blends of chitosan, poly(N-vinylpyrrolidone) (PVP) and titanium dioxide (TiO2) were investigated by Fourier transform infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). The size distribution of the TiO2 nanoparticles was measured using transmission electron microscope and scanning electron microscope. The studies on the mechanical properties of composite material indicate that the addition of TiO2 nanoparticles increases its strength. The prepared nanocomposite dressing has excellent antimicrobial efficacy and good biocompatibility against NIH3T3 and L929 fibroblast cells. Compared to conventional gauze, soframycin skin ointment and chitosan treated groups, the prepared nano dressing caused an accelerated healing of open excision type wounds in albino rat model. The synergistic effects of nanocomposite dressing material like good antibacterial ability, high swelling properties, high WVTR, excellent hydrophilic nature, biocompatibility, wound appearance and wound closure rate through in vivo test makes it a suitable candidate for wound healing applications. PMID:23618304

Archana, D; Singh, Brijesh K; Dutta, Joydeep; Dutta, P K

2013-06-01

231

Possibility of wound dressing using poly(L-leucine)/poly(ethylene glycol)/poly(L-leucine) triblock copolymer.  

PubMed

ABA-type block copolymers (abbreviated as LEL) composed of poly(L-leucine) (PLL) as the A component and poly(ethylene glycol) (PEG) as the B component were synthesized by ring-opening polymerization of L-leucine N-carboxyanhydride initiated by primary amino group located at both ends of PEG chain. A silver sulfadiazine (AgSD)-impregnated wound dressing of sponge type was prepared by the lyophilization method. Morphological structure of this wound dressing by scanning electron microscopy was observed to be composed of a dense skin layer and a porous inner layer. Equilibrium water content of LEL wound dressing increased with an increase in PEG content in the block copolymer due to the hydrophilicity of PEG. AgSD release from AgSD-impregnated wound dressing in PBS buffer (pH = 7.4) was dependent on PEG content in the block copolymer. Release of AgSD was increased in proportion to the PEG content in the copolymer. Antibacterial capacity of AgSD-impregnated wound dressing was examined in agar plate against Pseudomonas aeruginosa and Staphylococcus aureus. It was found that the suppression of bacterial proliferation in the wound dressing was dependent upon the PEG content. In cytotoxicity test, cell damage did not occur by the release of AgSD from the LEL sponge matrix of AgSD-medicated wound dressing. In in vivo test, granulous tissue formation and wound contraction for the AgSD- and dehydroepiandrosterone-impregnated LEL-2 wound dressing were faster than for any other groups. PMID:10632395

Kim, H J; Choi, E Y; Oh, J S; Lee, H C; Park, S S; Cho, C S

2000-01-01

232

Active and passive distraction in children undergoing wound dressings.  

PubMed

The aim of this study was to test how distraction influences pain, distress and anxiety in children during wound care. Sixty participants aged 5-12 years were randomized to three groups: serious gaming, the use of lollipops and a control group. Self-reported pain, distress, anxiety and observed pain behaviour were recorded in conjunction with wound care. Serious gaming, an active distraction, reduced the observed pain behaviour and self-reported distress compared with the other groups. A sense of control and engagement in the distraction, together, may be the explanation for the different pain behaviours when children use serious gaming. PMID:22819747

Nilsson, Stefan; Enskär, Karin; Hallqvist, Carina; Kokinsky, Eva

2013-04-01

233

Chemical and physical properties of a hydrogel wound dressing.  

PubMed

Geliperm hydrogel provides optimal physiological conditions for wound healing. The material is composed of two interlaced networks, one of polyacrylamide and one of agar, and contains about 96% firmly bound water. It is supplied in smooth, elastic, transparent sheets which are impermeable to bacteria but permeable to gases, salts, metabolites and proteins. Geliperm is nontoxic and has no irritative properties. Mechanical properties, water retention and diffusion of dyes and proteins are reported. Bacterial size should preclude penetration of the gel. The hydrogel in granular form represents a coherent material which could be used in deep fissured wounds and for the treatment of injuries with a large amount of exudation and contamination. PMID:2420380

Kickhöfen, B; Wokalek, H; Scheel, D; Ruh, H

1986-01-01

234

Evaluation of an oxygen-diffusion dressing for accelerated healing of donor-site wounds.  

PubMed

Accelerating the healing process and reducing pain during healing are beneficial for the following reasons: faster return to work, lower risk of wound infection, improved quality of life, and possibly reduced need for analgesia. This clinical study assessed the effectiveness of a new oxygen-diffusion dressing (OxyBand; Oxyband Technologies, St. Louis, MO) compared with standard Xeroform gauze dressings (Convidien, Mansfield, MA), in the care of skin-graft donor sites in burn patients. Time to healing was the primary endpoint, and pain scores and cosmetic outcome were also assessed. This was a prospective, randomized, controlled study of burn patients undergoing harvesting of two donor sites. Patients were followed at predetermined time points for 30 to 45 days to determine the time to reepithelialization, cosmetic appearance, and pain. Subjects were adult burn patients with less than 30% TBSA burns admitted to the burn center, who required excision and grafting. Twenty patients were enrolled, of whom 17 completed the study. Average age was 35 years. Average burn size was 9.2% TBSA. Patients underwent harvesting of split-thickness skin grafts with one donor wound dressed with OxyBand and the other dressed in Xeroform gauze. Wounds were inspected and photographed on postoperative days 4 and 8, and then every 2 days until the donor wounds were healed. Pain scores at each site were also collected at these visits (rated by patients on a scale from 0 to 10). Mean time to wound healing for OxyBand was 9.3 ± 1.7 days; for Xeroform, 12.4 ± 2.7 days (P < .001). Pain scores were lower (P < .01) at the OxyBand site compared with the Xeroform site at all time points during postoperative days 4 to 12. There was no difference in the cosmetic outcome of the wounds at 30 to 45 days postoperatively. This study revealed a decrease in the time to healing and in pain at donor sites dressed with an oxygen-diffusion dressing. PMID:23877142

Lairet, Kimberly F; Baer, David; Leas, Michelle L; Renz, Evan M; Cancio, Leopoldo C

2014-01-01

235

The impact of Manuka honey dressings on the surface pH of chronic wounds.  

PubMed

Chronic non healing wounds have an elevated alkaline environment. The acidic pH of Manuka honey makes it a potential treatment for lowering wound pH, but the duration of effect is unknown. Lowering wound pH can potentially reduce protease activity, increase fibroblast activity and increase oxygen release consequently aiding wound healing. The aim of this study was to analyse the changes in surface pH and size of non healing ulcers following application of Manuka honey dressing after 2 weeks. The study was an open label, non randomised prospective study. Patients presenting consecutively with non healing chronic superficial ulcers, determined by aetiology and no reduction in wound size in previous 3 weeks. Single pH measurements recorded using Blueline 27 glass surface electrode and R 315 pH meter set (Reagecon/Alkem, Co. Clare Ireland). Area determined using Visitrak (Smith & Nephew, Mull, UK) digital planimetry. Apinate (Manuka honey) (Comvita, Slough, UK) applied to wounds for 2 weeks after which wounds re-evaluated. Eight males and nine females with 20 ulcers (3 bilateral) were included: venous, 50% (n = 10); mixed aetiology, 35% (n = 7); arterial, 10% (n = 2) and pressure ulcer, 5% (n = 1). Reduction in wound pH after 2 weeks was statistically significant (P < 0.001). Wounds with pH >or= 8.0 did not decrease in size and wounds with pH wound size (P < 0.012). The use of Manuka honey dressings was associated with a statistically significant decrease in wound pH and a reduction in wound size. Elevated pH readings at the start were associated with minimal reduction in size. Surface wound pH measurements may contribute to objective wound assessments, but further research is necessary to determine its exact contribution. PMID:18494624

Gethin, Georgina T; Cowman, Seamus; Conroy, Ronan M

2008-06-01

236

Early Stage Treatment of Compartment Syndrome Using Polymer Sol-Gel Composite Growth Factor Delivery Wound Dressings.  

National Technical Information Service (NTIS)

Compartment syndrome (CS) as a result of blast or traumatic injury is a devastating problem in the battlefield. The ultimate goal of this study is to develop an integrated toolkit of novel, biodegradable wound dressing composites for early stage treatment...

C. Knabe H. Qu J. Kim P. Ducheyne S. Radin

2008-01-01

237

21 CFR 878.4015 - Wound dressing with poly (diallyl dimethyl ammonium chloride) (pDADMAC) additive.  

Code of Federal Regulations, 2013 CFR

...FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4015 Wound dressing with poly (diallyl dimethyl ammonium chloride) (pDADMAC)...

2013-04-01

238

In vitro microbial inhibition and cellular response to novel biodegradable composite wound dressings with controlled release of antibiotics  

Microsoft Academic Search

About 70% of all people with severe burns die from related infections, despite advances in treatment regimens and the best efforts of nurses and doctors. Although silver-eluting wound dressings are available for addressing this problem, there is growing evidence of the deleterious effects of such dressings in delaying the healing process owing to cellular toxicity. A new concept of antibiotic-eluting

J. J. Elsner; I. Berdicevsky; M. Zilberman

2011-01-01

239

In vitro assessment of water vapour transmission of synthetic wound dressings.  

PubMed

The water vapour transmission rate (WVTR) of 14 commercially available wound dressings (11 hydrocolloids, 2 hydrogels and 1 polyurethane film) was evaluated over 24 and 48 h periods using a modified ASTM standard method (ASTM E96-90). For the 48 h studies a novel microcomputer-controlled apparatus was employed. The dressings exhibited a wide range of WVTRs (76-9360 g m-2 d-1 at 24 h under forced air convection of 0.4 m s-1). The influence of air velocity of 0.4 m s-1 was not significant if the WVTR of the dressing was less than 880 g m-2 d-1 when measured under static air conditions. The influence of outer barrier layers and additional bandages on WVTR was also examined. PMID:7748992

Wu, P; Fisher, A C; Foo, P P; Queen, D; Gaylor, J D

1995-02-01

240

A Comparative Study of the Burn Wound Healing Properties of Saline-Soaked Dressing and Silver Sulfadiazine in Rats  

Microsoft Academic Search

The purpose of this study was to further investigate that phenomenon and to explore the effect silver sulfadiazine on wound\\u000a healing. Full-thickness burn wounds were created on the dorsum of Wistar albino rats under anesthesia. The wounds were treated\\u000a with silver sulfadiazine and saline-soaked dressing for fourteen days, and then observed until healed. Wound surface area\\u000a was measured each three

Hemmat Maghsoudi; Siavash Monshizadeh; Mehran Mesgari

2011-01-01

241

Preparation of carboxymethyl cellulose sulfates and its application as anticoagulant and wound dressing.  

PubMed

Tissue engineering is aiming to build an artificial environment or biological scaffold material that imitates the living environment of cells in the body. In this work, carboxymethyl cellulose sulfates were prepared by reacting carboxymethyl cellulose with N(SO3Na)3 which was synthesized by sodium bisulfite and sodium nitrite in aqueous solution. The reaction conditions affected the degree of substitution (DS) were measured by the barium sulfate nephelometry method. And the anticoagulant activity of carboxymethyl cellulose sulfates with different DS, concentration and molecular weights were investigated by the activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT). In addition, the effect of carboxymethyl cellulose sulfates on wound healing had been evaluated by the rate of wound healing and the histological examinations. The results indicated that the introduction of sulfate groups into the carboxymethyl cellulose sulfates improved its anticoagulant activity, and the wound dressings treated with carboxymethyl cellulose sulfates obviously promoted wound healing. PMID:24582936

Fan, Lihong; Zhou, Xiaoyu; Wu, Penghui; Xie, Weiguo; Zheng, Hua; Tan, Wang; Liu, Shuhua; Li, Qingyuan

2014-05-01

242

Preparation of gel film from Bombyx mori silk sericin and its characterization as a wound dressing.  

PubMed

Sericin is a highly hydrophilic protein family acting as the glue in Bombyx mori silk. In order to apply sericin as a wound dressing, a novel sericin film named gel film was prepared by a simple process without using any chemical modifications: sericin solution was gelled with ethanol into a sheet shape and then dried. Infrared analysis revealed that the sericin gel film contained water-stable beta-sheet networks formed in the gelation step. This structural feature rendered the gel film morphologically stable against swelling and gave it good handling properties in the wet state. The sericin gel film rapidly absorbed water, equilibrating at a water content of about 80%, and exhibited elastic deformation up to a strain of about 25% in the wet state. A culture of mouse fibroblasts on the gel film indicated that it had low cell adhesion properties and no cytotoxicity. These characteristics of sericin gel film suggest its potential as a wound dressing. PMID:19060395

Teramoto, Hidetoshi; Kameda, Tsunenori; Tamada, Yasushi

2008-12-01

243

The effect of tea tree oil (Melaleuca alternifolia) on wound healing using a dressing model.  

PubMed

Numerous studies have shown the promising antibacterial effects of Melaleuca alternifolia, or tea tree essential oil. The study detailed here replicates in humans a 2004 in vitro study that used a dressing model over Petri dishes to determine the antimicrobial effects of the fumes of tea tree essential oil. The current study used the same dressing model with patients who had wounds infected with Staphylococcus aureus. Ten participants volunteered for the quasi-experimental study, and four of the 10 were used as matched participants to compare wound healing times between conventional treatment alone and conventional treatment plus fumes of tea tree essential oil. The results demonstrated decreased healing time in all but one of the participants treated with tea tree oil. The differences between the matched participants were striking. The results of this small investigational study indicate that additional study is warranted. PMID:23848210

Chin, Karen B; Cordell, Barbara

2013-12-01

244

The physical effects of an adhesive dressing top layer on burn wound dressings.  

PubMed

Three hydrogels were assessed in respect of water-vapour transmission and conformability. These materials were tested alone and as part of a bilaminate dressing which incorporates an adhesive top layer. The adhesive layer used in this study was a compliant adhesive bandage, marketed under the name Mefix. It was observed that this layer reduced the normally high water-vapour transmission rates of the hydrogels to much lower, clinically more acceptable, levels. Mechanically, the Mefix layer protects the underlying hydrogel, prevents tearing and puncturing but decreases conformability by elastic restraint. PMID:3730913

Queen, D; Evans, J H; Gaylor, J D; Courtney, J M; Reid, W H

1986-06-01

245

[Local treatment of burn injuries and traumatic wounds with a hydrogel Geliperm dressing].  

PubMed

Hydrogel Geliperm dressings of BYK Goldbin - Konstanz and Geistlich Sons Ldt. firms were used in patients in case of skin burns and some traumatic wounds. During a four-year observation it was discovered that those materials are safe for the patient, their removing is painless and their are fully useful in the treatment of burns and traumatic wounds in surgical departments and in the ambulatory treatment. Geliperm does not have allergic action. Because of its physicochemical and biological properties Geliperm may have also usage for example in the treatment of leg ulcerations, of bed-sores and of radiation skin defect. PMID:3268831

Ku?, H; Misterka, S; Pielka, S; Juszkiewicz, M

1988-01-01

246

Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound healing.  

PubMed

Materials able to deliver topically bioactive molecules represent a new generation of biomaterials. In this article, we describe the use of silk mats, made of electrospun nanoscale silk fibers containing epidermal growth factor (EGF), for the promotion of wound healing processes. In our experiments, we demonstrated that EGF is incorporated into the silk mats and slowly released in a time-dependent manner (25% EGF release in 170h). We tested these materials using a new model of wounded human skin-equivalents displaying the same structure as human skin and able to heal using the same molecular and cellular mechanisms found in vivo. This human three-dimensional model allows us to demonstrate that the biofunctionalized silk mats, when placed on the wounds as a dressing, aid the healing by increasing the time of wound closure by the epidermal tongue by 90%. The preservation of the structure of the mats during the healing period as demonstrated by electronic microscopy, the biological action of the dressing, as well as the biocompatibility of the silk demonstrate that this biomaterial is a new and very promising material for medical applications, especially for patients suffering from chronic wounds. PMID:19162575

Schneider, A; Wang, X Y; Kaplan, D L; Garlick, J A; Egles, C

2009-09-01

247

Silver-doped self-assembling di-phenylalanine hydrogels as wound dressing biomaterials.  

PubMed

Chronic and acute wounds can be quickly contaminated and infected by microorganisms such as bacteria, multi-resistant organisms or fungi. The introduction of silver as anti-microbial agent into wound management has widely been demonstrated to be effective and contribute to wound healing. As a consequence, many approaches and different materials have been employed to synthesize antibacterial silver-hydrogels. In this work the introduction of silver particles into the fibrillar structure of self-assembling aromatic di-phenylalanine derivatives modified with aromatic groups such as 9-fluorenylmethoxycarbonyl is proposed to produce antibacterial wound dressings. Hydrogels doped with increasing amounts of silver were tested and adopted to modify flax textiles. The influence of silver on the structure of hydrogels was studied using light and confocal microscopy, while SEM-EDX allowed the characterization of the hydrogel coating on the surface of the textile substrates as well as the identification and distribution of silver nanoparticles. The antibacterial potential of the treated flax was demonstrated through microbiological tests on Staphylococcus aureus. The combination of the physico-chemical and anti-bacterial properties, together with the ease of preparation of these biomaterials, fulfils the requirement of clinically-effective wound dressings. PMID:23793492

Paladini, Federica; Meikle, S T; Cooper, I R; Lacey, J; Perugini, V; Santin, M

2013-10-01

248

The use of honey as a topical dressing to treat a large, devitalized wound in a stumptail macaque (Macaca arctoides).  

PubMed

There are many reasons wounds are managed as open wounds rather than by primary closure. Indications include gross contamination, infection, and skin loss leading to insufficient adjacent tissue for wound closure. The most common method of managing an open wound is with wet-to-dry dressings. Wet-to-dry dressings provide mechanical debridement and promote the movement of viscous exudates away from the wound. Wet-to-dry bandages ideally are changed every 12 to 24 h. For nonhuman primates, it is desirable to develop wound management techniques that limit animal handling for bandage changes and thus the frequency of sedation. Anecdotal reports on the use of honey to treat wounds date back to 2000 B.C. Recently, scientific inquiries have found merit to these reports. Honey accelerates healing because of its direct effects on tissue and antibacterial properties. In addition, dressings with honey can be changed relatively infrequently. Honey decreases inflammatory edema, hastens sloughing of devitalized tissue, attracts macrophages which cleanse the wound, provides a local cellular energy source, and protectively covers the wound. A high osmolarity, acidity, and hydrogen peroxide content confer honey with antibacterial properties. Here we describe the use of honey to manage a bite wound in a stumptail macaque (Macaca arctoides). The wound healed rapidly: after 2 weeks of treatment, there was markedly less exudate and no necrotic tissue. This report describes how honey may be helpful in the management of open wounds in nonhuman primates by minimizing the need for sedation for bandage changes. PMID:16050668

Staunton, Christine J; Halliday, Lisa C; Garcia, Kelly D

2005-07-01

249

Efficiency of Microbial Cellulose Dressing in Partial-Thickness Burn Wounds  

Microsoft Academic Search

Microbial cellulose is a natural polymer that can hold a quantity of water without any disconformities. Therefore, it is proposed for use as wound dressing material. We report a case of 28% total body surface area partial-thickness flame burn: approximately 4.5% superficial partial-thickness burns on anterior face and 23.5% combined superficial and deep partial-thickness burns on both upper arms and

Pornprom Muangman; Supaporn Opasanon; Supaparn Suwanchot; Orapin Thangthed

2011-01-01

250

Electron beam crosslinked PEO and PEO\\/PVA hydrogels for wound dressing  

Microsoft Academic Search

In order to prepare polyethylene oxide (PEO) hydrogel for wound dressing, different molecular weight PEO and PEO\\/poly(vinyl alcohol), PVA blend hydrogels were obtained with electron beam irradiation. Gel formation of PEO in aqueous solution was saturated at 40 kGy and the achieved gel fraction was 60–70%. The PEO hydrogel obtained was very fragile, hence PVA was added at 10–30% to

F. Yoshii; Y. Zhanshan; K. Isobe; K. Shinozaki; K. Makuuchi

1999-01-01

251

Preparation and properties of electrospun poly(vinyl alcohol)\\/silver fiber web as wound dressings  

Microsoft Academic Search

þ ions in the electrospun PVA\\/AgNO3 fiber web into the Ag nanoparticles. Also the heat treatment improved the crystallinity of the electrospun PVA fiber web and so it made the web unsolved in moisture environment. Therefore, it was concluded that the only heat treated electrospun PVA\\/AgNO3 fiber web was a good material as wound dressings because it had structural stability

Kyung Hwa Hong

2007-01-01

252

Mechanical, Bioadhesive Strength and Biological Evaluations of Chitosan films for Wound Dressing  

Microsoft Academic Search

Purpose. To investigate the suitability of chitosan films prepared using two different solvents, acetic acid (Chitosan-AA) and lactic acid (Chitosan- LA), for wound dressing, in comparison with a com- mercial preparation, Omiderm?. Methods. The mechanical and in-vitro bioadhesive strength proper- ties of Chitosan-AA, Chitosan-LA, and Omiderm? were investigated using texture analyzer equipment. The vapour permeability of chitosan films was deter-

Tanveer Ahmad Khan; Kok Khiang Peh; Hung Seng; Ch' ng

253

Wide Topical Negative Pressure Wound Dressing Treatment for Patients Undergoing Abdominal Dermolipectomy Following Massive Weight Loss  

Microsoft Academic Search

Postbariatric plastic surgery is considered to be a high-risk procedure, which entails such frequent minor complications as\\u000a postoperative seroma, bleeding and wound dehiscence. These occur with a high incidence, especially, following postbariatric\\u000a abdominal dermolipectomy. In order to reduce these complication rates, a new type of dressing with wide abdominal topical\\u000a negative pressure (TNP) application was applied. We performed abdominal dermolipectomy

Adrian Dragu; Stefan Schnürer; Frank Unglaub; Maya B. Wolf; Justus P. Beier; Ulrich Kneser; Raymund E. Horch

254

Evaluation of sericin\\/collagen membranes as prospective wound dressing biomaterial  

Microsoft Academic Search

Sericin, a silk protein, has high potential for use in biomedical applications. In this study, wound dressing membranes of Sericin (S) and Collagen (C) were prepared by glutaraldehyde cross-linking at S\\/C; 2:1, 1:1, 1:2, and 0:1 weight ratios. They were stable in water for 4weeks. However, increasing the proportion of sericin had decreasing effect on the membrane stability. Water swelling

Omer Akturk; Aysen Tezcaner; Hasan Bilgili; M. Salih Deveci; M. Rusen Gecit; Dilek Keskin

2011-01-01

255

An in vitro assessment of MRI issues at 3-Tesla for antimicrobial, silver-containing wound dressings?.  

PubMed

Although no reports of adverse events have been published to date, the presence of metallic dressing ingredients may present an magnetic resonance imaging (MRI) safety concern for patients using silver-containing wound dressings. The purpose of this in vitro study was to test magnetic field interactions (ie, translational attraction and torque), heating, artifacts, and conductivity (ie, electrical resistance) when using MRI at 3-Tesla for two (nonborder and border) silver-containing wound dressings. The results indicated the dressings displayed no magnetic field interactions (deflection angle 0?; no torque), and in each case, MRI-related heating effects were at the same levels as the background temperature increases (ie, <1.8?C). The dressings created extremely subtle artifacts (one-for-one relationship) on the MR images. With regard to the conductivity assessments, the average resistance values were 20 kOhm and 1.1 kOhm, respectively, for the nonborder and border wound dressings, which were acceptable levels. The findings show the two silver-containing wound dressings tested will not pose hazards or risks to patients and, thus, are considered "MR safe" according to the current labeling terminology used for medical products, and each dressing may be left in place when a patient undergoes an MRI examination. To date, only a hydrofiber silver-containing dressing has been tested for MRI safety. Because of potential variances in material characteristics, MRI test results are specific to the dressings tested and cannot be applied to other products. Future studies to define the level of silver concentration in dressings that may pose a hazard for performing an MRI are warranted. PMID:23134899

Escher, Kirin B; Shellock, Frank G

2012-11-01

256

Pilot scale-up and shelf stability of hydrogel wound dressings obtained by gamma radiation  

NASA Astrophysics Data System (ADS)

This study is aimed of producing pilot batches of hydrogel wound dressings by gamma radiation and evaluating their shelf stability. Six batches of 3L capacity were prepared based on poly(vinyl pyrrolidone), agar and polyethylene glycol and they were dispensed in polyester trays, covered with polyester films and sealed in two types of materials: polyethylene bags and vacuum polyethylene bags. Dressings were formed in a single step process for the hydrogel formation and sterilization at 25-30 kGy gamma radiation dose in a JS-9500 Gamma Irradiator (Nordion, Canada). The six batches were initially physicochemical characterized in terms of dimensions and appearance, gel fraction, morphology analysis, hydrogel strength, moisture retention capability and swelling capacity. They were kept under two storage conditions: room temperature (T: 30±2 °C/RH: 70± 5%) and refrigerated temperature (T: 5±3 °C) during 24 months and sterility test was performed. The appearance of membranes was transparent, clear, uncut and flexible; the gel fraction of batches was higher than 75% and the hydrogel surface showed a porous structure. There was a slow decrease of the compression rate 20% until 7 h and about 70% at 24 h. Moisture retention capability in 5 h was similar for all the batches, about 40% and 60% at 37 °C and at room temperature respectively. The swelling of hydrogels in acidic media was strong and in alkaline media the weight variation remains almost stable until 24 h and then there is a loss of weight. The six batches remained sterile during the stability study in the conditions tested. The pilot batches were consistent from batch to batch and remained stable during 24 months.

Soler, Dulce María; Rodríguez, Yanet; Correa, Hector; Moreno, Ailed; Carrizales, Lila

2012-08-01

257

Gel characterisation and in vivo evaluation of minocycline-loaded wound dressing with enhanced wound healing using polyvinyl alcohol and chitosan.  

PubMed

The purpose of this study was to develop a minocycline-loaded wound dressing with an enhanced healing effect. The cross-linked hydrogel films were prepared with polyvinyl alcohol (PVA) and chitosan using the freeze-thawing method. Their gel properties, in vitro protein adsorption, release, in vivo wound healing effect and histopathology were then evaluated. Chitosan decreased the gel fraction, maximum strength and thermal stability of PVA hydrogel, while it increased the swelling ability, water vapour transmission rate, elasticity and porosity of PVA hydrogel. Incorporation of minocycline (0.25%) did not affect the gel properties, and chitosan hardly affected drug release and protein adsorption. Furthermore, the minocycline-loaded wound dressing composed of 5% PVA, 0.75% chitosan and 0.25% drug was more swellable, flexible and elastic than PVA alone because of relatively weak cross-linking interaction of chitosan with PVA. In wound healing test, this minocycline-loaded PVA-chitosan hydrogel showed faster healing of the wound made in rat dorsum than the conventional product or the control (sterile gauze) due to antifungal activity of chitosan. In particular, from the histological examination, the healing effect of minocycline-loaded hydrogel was greater than that of the drug-loaded hydrogel, indicating the potential healing effect of minocycline. Thus, the minocycline-loaded wound dressing composed of 5% PVA, 0.75% chitosan and 0.25% drug is a potential wound dressing with excellent forming and enhanced wound healing. PMID:20230884

Sung, Jung Hoon; Hwang, Ma-Ro; Kim, Jong Oh; Lee, Jeong Hoon; Kim, Yong Il; Kim, Jeong Hoon; Chang, Sun Woo; Jin, Sung Giu; Kim, Jung Ae; Lyoo, Won Seok; Han, Sung Soo; Ku, Sae Kwang; Yong, Chul Soon; Choi, Han-Gon

2010-06-15

258

Impact of gauze-based NPWT on the patient and nursing experience in the treatment of challenging wounds.  

PubMed

Negative pressure wound therapy is widely used in the treatment of hard-to-heal wounds; however, pain during dressing changes, which is often associated with pain on the commencement and cessation of pressure application and because of in-growth of new granulation tissue into interstices of foam dressings, is often experienced. Anecdotal reports have suggested that choice of gauze as the negative pressure wound therapy dressing may reduce the pain associated with dressing changes. A prospective, multi-center, non-comparative clinical investigation was carried out using gauze-based negative pressure wound therapy in chronic and acute wounds. Over 152 patients were evaluated. Median duration of therapy was 18 days with 91% of patients progressing towards healing at the end of therapy. Wound pain and odour were significantly reduced (P < 0.001) over the course of therapy. Wound pain during dressing changes was reported to be absent in 80% of dressing removals. No damage to the wound bed following dressing removal was observed in 96% of dressing changes. Dressing applications were considered easy in 79% of assessments and took a median of 20 min to complete. In patients susceptible to pain, gauze-based negative pressure therapy may be a viable option to maximise patient comfort. PMID:20673256

Hurd, Theresa; Chadwick, Paul; Cote, Julien; Cockwill, John; Mole, Trevor R; Smith, Jennifer M

2010-12-01

259

Tissue engineered plant extracts as nanofibrous wound dressing.  

PubMed

Use of plant extracts for treatment of burns and wound is a common practice followed over the decades and it is an important aspect of health management. Many medicinal plants have a long history of curative properties in wound healing. Electrospun nanofibers provide high porosity with large surface area-to-volume ratio and are more appropriate for cell accommodation, nutrition infiltration, gas exchange and waste excretion. Electrospinning makes it possible to combine the advantages of utilizing these plant extracts in the form of nanofibrous mats to serve as skin graft substitutes. In this study, we investigated the potential of electrospinning four different plant extracts, namely Indigofera aspalathoides, Azadirachta indica, Memecylon edule (ME) and Myristica andamanica along with a biodegradable polymer, polycaprolactone (PCL) for skin tissue engineering. The ability of human dermal fibroblasts (HDF) to proliferate on the electrospun nanofibrous scaffolds was evaluated via cell proliferation assay. HDF proliferation on PCL/ME nanofibers was found the highest among all the other electrospun nanofibrous scaffolds and it was 31% higher than the proliferation on PCL nanofibers after 9 days of cell culture. The interaction of HDF with the electrospun scaffold was studied by F-actin and collagen staining studies. The results confirmed that PCL/ME had the least cytotoxicity among the different plant extract containing scaffolds studied here. Therefore we performed the epidermal differentiation of adipose derived stem cells on PCL/ME scaffolds and obtained early and intermediate stages of epidermal differentiation. Our studies demonstrate the potential of electrospun PCL/ME nanofibers as substrates for skin tissue engineering. PMID:23111334

Jin, Guorui; Prabhakaran, Molamma P; Kai, Dan; Annamalai, Sathesh Kumar; Arunachalam, Kantha D; Ramakrishna, Seeram

2013-01-01

260

Novel soy protein wound dressings with controlled antibiotic release: mechanical and physical properties.  

PubMed

Naturally derived materials are becoming widely used in the biomedical field. Soy protein has advantages over various types of natural proteins employed for biomedical applications due to its low price, non-animal origin and relatively long storage time and stability. In the current study soy protein isolate (SPI) was investigated as a matrix for wound dressing applications. The antibiotic drug gentamicin was incorporated into the matrix for local controlled release and, thus, protection against bacterial infection. Homogeneous yellowish films were cast from aqueous solutions. After cross-linking they combined high tensile strength and Young's modulus with the desired ductility. The plasticizer type, cross-linking agent and method of cross-linking were found to strongly affect the tensile properties of the SPI films. Selected SPI films were tested for relevant physical properties and the gentamicin release profile. The cross-linking method affected the degree of water uptake and the weight loss profile. The water vapor transmission rate of the films was in the desired range for wound dressings (?2300 g m(-2) day(-1)) and was not affected by the cross-linking method. The gentamicin release profile exhibited a moderate burst effect followed by a decreasing release rate which was maintained for at least 4 weeks. Diffusion was the dominant release mechanism of gentamicin from cross-linked SPI films. Appropriate selection of the process parameters yielded SPI wound dressings with the desired mechanical and physical properties and drug release behavior to protect against bacterial infection. These unique structures are thus potentially useful as burn and ulcer dressings. PMID:21911084

Peles, Zachi; Zilberman, Meital

2012-01-01

261

Control of multi-drug-resistant pathogens with non-thermal-plasma-treated alginate wound dressing.  

PubMed

Abstract Background: Non-thermal dielectric-barrier discharge plasma (non-thermal plasma) is being investigated for use in wound healing. Alginate gel, already in clinical use, is non-toxic but has no meaningful antimicrobial property. This study reports that a non-thermal-plasma-treated alginate wound dressing has strong antimicrobial properties. Methods: Alginate gel was treated with non-thermal plasma in room air and inoculated with bacterial pathogens. At 15?min after this, bacterial cell viability was determined by colony assay or 2,3-bis-(2-methoxy-4- nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. The anti-biofilm efficacy of the non-thermal-plasma-treated alginate gel was investigated and the treated gel was tested against vascular endothelial cells for a cytotoxic effect. The proliferation and migration of bacterial cells before and after exposure to the treated gel were investigated with an in vitro wound testing assay. Scanning electron microscopy was used to observe changes in the gel surface associated with exposure to bacterial pathogens. The treated gel was tested against Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus, S. epidermidis, Candida albicans, and C. glabrata as representative pathogens (at 10(6)-10(9) colony-forming units [CFU]/mL), and the thickness of a plasma-treated gel dressing and distance between a glass dielectric-barrier discharge plasma probe and the gel surface were kept constant. Results: Non-thermal-plasma-treated alginate gel exhibited a strong biocidal property and inactivated all of the pathogens included in the study at counts of 10(8) CFU/mL and within 15?sec of treatment. The treated gel inactivated 10(9) CFU/mL of the organisms within 1?min, and 3 min of exposure to the treated gel inactivated pathogens embedded in biofilms. The plasma-treated gel showed no significant cytotoxicity, and endothelial cells exposed to the treated gel proliferated and migrated well across a wound area over a period of time. Dressings made with the treated gel retained their biocidal effects for about a month. Scanning electron microscopy showed no damage to the surfaces of treated gels, but damage to the bacterial pathogens on plasma exposure. Conclusion: A non-thermal-plasma-treated alginate gel dressing has the clinical potential to decontaminate wounds, prevent surgical site infection, and promote wound healing. PMID:24749699

Poor, Alexander E; Ercan, Utku K; Yost, Adam; Brooks, Ari D; Joshi, Suresh G

2014-06-01

262

An in vitro method for the quantitative determination of the antimicrobial efficacy of silver-containing wound dressings.  

PubMed

Treatment with silver-containing wound dressings is becoming an increasingly popular strategy to eliminate growth of opportunistic wound pathogens during the healing process. However, there are concerns over the possible side-effects of silver to the patient; coupled to the cost of silver as an ingredient there is a desire to ensure that wound dressings contain the least quantity of active ingredient to ensure the minimum bactericidal concentration (MBC) of silver is maintained in the wound environment. This requires the ability to determine the efficacy of silver directly within the wound environment; an extremely complicated task that is difficult using classical (plate counting) microbiological assays because these cannot be conducted in situ. Here, we report a quantitative method for determining the efficacy of silver in wound dressings using an isothermal calorimetric method. The growth curves of P. aeruginosa (NCIMB 8628) were recorded in growth medium and in growth medium containing AQUACEL Ag Hydrofiber dressing. It was found that 10 mg of dressing was sufficient to ensure no detectable growth of organism in 2.5 mL of medium inoculated to 10(6) cfu/mL. This corresponded to a silver load of 1.1x10(-6) moles (equivalent to 4.4x10(-4) M, in the volume of medium used in the experiment). Experiments conducted with silver nitrate rather than dressing indicated the MBC of silver against P. aeruginosa was 1x10(-4) M. The results suggested that not all of the silver in the dressing was bioavailable, at least over the lifetime of the experiment. One advantage of this effect would be the lack of excess availability of the silver, which allays fears of potential toxicity to the patient and may provide an extended period of time over which the dressing is bactericidal. PMID:18832021

Gaisford, Simon; Beezer, Anthony E; Bishop, Alistair H; Walker, Michael; Parsons, David

2009-01-21

263

Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds.  

PubMed

Silver products have been used for thousands of years for their beneficial effects, often for hygiene and in more recent years as antimicrobials on wounds from burns, trauma, and diabetic ulcers. Silver sulfadiazine creams (Silvazine and Flamazine) are topical ointments that are marketed globally. In recent years, a range of wound dressings with slow-release Ag compounds have been introduced, including Acticoat, Actisorb Silver, Silverlon, and others. While these are generally accepted as useful for control of bacterial infections (and also against fungi and viruses), key issues remain, including importantly the relative efficacy of different silver products for wound and burn uses and the existence of microbes that are resistant to Ag+. These are beneficial products needing further study, although each has drawbacks. The genes (and proteins) involved in bacterial resistance to Ag have been defined and studied in recent years. PMID:16761169

Silver, Simon; Phung, Le T; Silver, Gregg

2006-07-01

264

Severe wound traction-blisters after inadequate dressing application following laparoscopic cholecystectomy: case report of a preventable complication  

PubMed Central

Background The inadequate application of postoperative dressings can lead to significant complications, including skin injuries, compartment syndromes, and potential limb loss. To our knowledge, the occurrence of post laparoscopic cholecystectomy related skin complications have not yet been reported in the peer-reviewed literature. Case Presentation Following laparoscopic cholecystectomy for symptomatic gallstone disease, a seventy eight year old healthy white male broke out in painful erythema on either side of his epigastric port site. Vesicles akin to a partial thickness burns were revealed upon removal of dressings. An unusual indentation created by the dressing, and skin traction by the dressing's adhesive edges were implicated, raising questions about technique of its application. Conclusion Incorrect application of wound dressings can disrupt skin architecture, causing painful blistering. This complication should not occur to patients, as it is theoretically 100% preventable. Avoidance of stretching adhesive dressings, and careful adherence to relevant manufacturers' instructions are recommended.

2011-01-01

265

Comparison of safety and efficacy of papaya dressing with hydrogen peroxide solution on wound bed preparation in patients with wound gape  

PubMed Central

Objective: Indian papaya or Carica papaya is known to have de-sloughing and wound-healing properties due to the presence of protease enzymes. The present study was done to compare the efficacy and safety of papaya dressing with hydrogen peroxide solution for preparation of wound bed in patients of postoperative wound gape. Materials and Methods: A randomized, open-labeled interventional study was carried out over a period of 8 months at a tertiary care hospital on post-caesarean section patients with wound gape. The efficacy parameters studied were duration of time required to induce development of healthy granulation tissue and total duration of hospitalization. Safety parameters studied were adverse effects reported by patients and development of hypersensitivity reaction. Results: A total of 64 patients were enrolled, of which 32 patients received hydrogen peroxide dressing and 31 patients received papaya dressing (one patient withdrew after randomization). Time required to induce the development of healthy granulation tissue and total duration of hospitalization were 6.2 ± 1.6 days vs 2.5 ± 0.5 days and 19.2 ± 5.8 days vs 12.92 ± 4.6 days in papaya and hydrogen peroxide dressing groups, respectively. Both primary efficacy parameters were significantly shorter in papaya dressing group. The incidence of adverse effects like local irritation and itching were comparable in both groups and the difference was not statistically significant. Conclusion: Papaya dressing is more efficacious and equally safe as compared to hydrogen peroxide dressing when used for wound bed preparation in patients with postoperative wound gape.

Murthy, Mangala B.; Murthy, Bhasker K.; Bhave, Sanjay

2012-01-01

266

Randomised clinical trial of Hydrofiber dressing with silver versus povidone-iodine gauze in the management of open surgical and traumatic wounds.  

PubMed

This prospective, randomised clinical trial compared pain, comfort, exudate management, wound healing and safety with Hydrofiber dressing with ionic silver (Hydrofiber Ag dressing) and with povidone-iodine gauze for the treatment of open surgical and traumatic wounds. Patients were treated with Hydrofiber Ag dressing or povidone-iodine gauze for up to 2 weeks. Pain severity was measured with a 10-cm visual analogue scale (VAS). Other parameters were assessed clinically with various scales. Pain VAS scores decreased during dressing removal in both groups, and decreased while the dressing was in place in the Hydrofiber Ag dressing group (n = 35) but not in the povidone-iodine gauze group (n = 32). Pain VAS scores were similar between treatment groups. At final evaluation, Hydrofiber Ag dressing was significantly better than povidone-iodine gauze for overall ability to manage pain (P < 0.001), overall comfort (P < or = 0.001), wound trauma on dressing removal (P = 0.001), exudate handling (P < 0.001) and ease of use (P < or = 0.001). Rates of complete healing at study completion were 23% for Hydrofiber Ag dressing and 9% for povidone-iodine gauze (P = ns). No adverse events were reported with Hydrofiber Ag dressing; one subject discontinued povidone-iodine gauze due to adverse skin reaction. Hydrofiber Ag dressing supported wound healing and reduced overall pain compared with povidone-iodine gauze in the treatment of open surgical wounds requiring an antimicrobial dressing. PMID:17425549

Jurczak, Florent; Dugré, Thierry; Johnstone, Alison; Offori, Theodor; Vujovic, Zorica; Hollander, Dirk

2007-03-01

267

Poly(n-vinylpyrrolidone) hydrogels: 2.Hydrogel composites as wound dressing for tropical environment  

NASA Astrophysics Data System (ADS)

POLY(N-VINYLPYRROLIDONE) HYDROGELS: 2. HYDROGEL COMPOSITES AS WOUND DRESSING FOR TROPICAL ENVIRONMENT. The effects of irradiation on hydration and other properties of poly(vinylpyrrolidone) (PVP) hydrogel composites have been investigated. The aqueous solution of vinylpyrrolidone (VP) 10 wt % was mixed with several additives such as agar and polyethylen glycol (PEG). The solution was then irradiated with gamma rays from Cobalt-60 source at room temperature. Several parameters such as elongation at break (EB), tensile strength (TS), degree of swelling (DS), water vapor transmission rate (WVTR), equilibrium water content (EWC), microbial growth and penetration test, and water activity (Aw) were analysed at room temperature of 29 ±2°C humidity of 80 ± 10%. Results show that elongation at break of hydrogel membranes with initial composition of VP with agar, VP with agar and PEG were 240 % and 250 % kGy, the equilibrium water content of membranes were 96 to 90%, whereas degree of swelling were 55 to 10. The WVTR of hydrogel membranes with initial composition of VP with agar and PEG was 70 g m -2h -1, while the water activity was 0.9. Such hydrogel membranes exhibits the following properties: They are elastic, transparent, flexible, impermeable for bacteria. They absopt a high capacity of water, attached to healthy skin but not to the wound and they are easy to remove. These properties of the hydrogel membranes allow for applying as a wound dressings in tropical environment.

Himly, N.; Darwis, D.; Hardiningsih, L.

1993-10-01

268

Evaluation of sericin/collagen membranes as prospective wound dressing biomaterial.  

PubMed

Sericin, a silk protein, has high potential for use in biomedical applications. In this study, wound dressing membranes of Sericin (S) and Collagen (C) were prepared by glutaraldehyde cross-linking at S/C; 2:1, 1:1, 1:2, and 0:1 weight ratios. They were stable in water for 4 weeks. However, increasing the proportion of sericin had decreasing effect on the membrane stability. Water swelling property of membranes was enhanced with sericin. The highest water swelling was obtained in 1:1 group (9.06 g/g), but increasing collagen or sericin content in the membranes had a diminishing effect. Highest water vapor transmission rate was obtained with 1:2 group (1013.80 g/m(2)/day). Oxygen permeability results showed that 1:2 (7.67 mg/L) and 2:1 (7.85 mg/L) S/C groups were better than the other groups. While sericin decreased the tensile strength and elongation of membranes, it increased modulus. Sericin also increased brittleness of membranes, but their UTS range (24.93-44.92 MPa) was still suitable for a wound dressing. Membranes were not penetrable to microorganisms. Cytotoxicity studies showed that fibroblasts and keratinocytes attached and gained their characteristic morphologies. They also proliferated on membranes significantly. After 1 week of subcutaneous implantation, a fibrous capsule formed around all membranes with an acute inflammation. Sericin containing membranes showed signs of degradation (at 2nd week), while collagen only membranes remained largely intact. Eventually, sericin containing membranes degraded in 3 weeks with moderate inflammatory response. Overall results suggest that sericin/collagen membranes would be favorable as wound dressing material when sericin ratio is less than or equal to the collagen component. PMID:21697006

Akturk, Omer; Tezcaner, Aysen; Bilgili, Hasan; Deveci, M Salih; Gecit, M Rusen; Keskin, Dilek

2011-09-01

269

Immobilization of derivatized dextran nanoparticles on konjac glucomannan/chitosan film as a novel wound dressing.  

PubMed

The aim of this study was to prepare konjac glucomannan (KGM)/chitosan (CS) film containing glycidyl methacrylate derivatized dextran (dex-GMA)/acrylic acid(AAc) nanoparticles loaded with antibacterial agent. In this study, An optimized procedure chosen from three methods was used to prepare Erythromycin (EM)-loaded poly(dex-GMA/AAc) nanoparticles and obtained nanoparticles ranged from 50-200 nm. Film was found to have equilibrium water content (EWC) 99.3% which could prevent exudates on wound bed from accumulating and also have excellent water adsorption 2362.3 +/- 55.2%; the water vapor transmission rate (WVTR) was 2335 +/- 36 gm(-2) day(-1) and evaporative water loss from the film (EWL) was approximately 10% after 1 h and within 6 h it increased to 90%. Drug release of film containing nanoparticles or absent was determined, within 22 h accumulative release was 40.3%, 72.5% respectively. In conclusion, KGM/CS film containing nanoparticles could not only maintain a moist environment over wound bed in moderate to heavily exuding wound but also provide a continuous and sustained release of the antibacterial agent on the wound surface, which could be potential wound dressing. PMID:18607072

Zhang, Hui; Gu, Chun-Hu; Wu, Hong; Fan, Li; Li, Fei; Yang, Fan; Yang, Qian

2007-01-01

270

Antimicrobial electrospun nanofibers of cellulose acetate and polyester urethane composite for wound dressing.  

PubMed

In this study, a series of nanofibrous membranes were prepared from cellulose acetate (CA) and polyester urethane (PEU) using coelectrospinning or blend-electrospinning. The drug release, in vitro antimicrobial activity and in vivo wound healing performance of the nanofiber membranes were evaluated for use as wound dressings. To prevent common clinical infections, an antimicrobial agent, polyhexamethylene biguanide (PHMB) was incorporated into the electrospun fibers. The presence of CA in the nanofiber membrane improved its hydrophilicity and permeability to air and moisture. CA fibers became slightly swollen upon contacting with liquid phase. CA not only increased the liquid uptake but also created a moist environment for the wound, which accelerated wound recovery. PHMB release dynamics of the membranes was controlled by the structure and component ratios of the membranes. The lower ratio of CA: PEU helped to preserve the physical and thermal properties of the membranes, and also reduced the burst release effectively and slowed down diffusion of PHMB during in vitro tests. The controlled-diffusion membranes exerted long-term antimicrobial effect for wound healing. PMID:22692845

Liu, Xin; Lin, Tong; Gao, Yuan; Xu, Zhiguang; Huang, Chen; Yao, Gang; Jiang, Linlin; Tang, Yanwei; Wang, Xungai

2012-08-01

271

A drug-free oral hydrogel wound dressing for pain management in immediate denture patients.  

PubMed

This article evaluated a drug-free oral hydrogel wound dressing composed entirely of natural food ingredients for its ability to relieve pain in immediate denture patients. Evaluation occurred at a 24-hour postoperative appointment. For this crossover study, 44 patients who were taking oral narcotics evaluated their discomfort (using a scale of 0-10) at 1, 3, 5, and 10 minutes after denture insertion with no topical treatment and again after SockIt! Oral Pain Gel was applied to the dentures. The gel provided statistically significant pain relief at all time points beyond that provided by oral narcotic alone (p < 0.0001). PMID:19903626

Kennedy, Thomas J; Hall, John E

2009-01-01

272

Development of salmon milt DNA\\/salmon collagen composite for wound dressing  

Microsoft Academic Search

This study aims to develop a novel wound dressing comprising salmon milt DNA (sDNA) and salmon collagen (SC). The sDNA\\/SC\\u000a composites were prepared by incubating a mixture of an acidic SC solution, an sDNA solution, and a collagen fibrillogenesis\\u000a inducing buffer (pH 6.8) containing a crosslinking agent (water-soluble carbodiimide) for gelation, and a subsequent ventilation-drying\\u000a process to give sDNA\\/SC films.

XuanRi Shen; Nobuhiro Nagai; Masaru Murata; Daisuke Nishimura; Masahito Sugi; Masanobu Munekata

2008-01-01

273

Acemannan-containing wound dressing gel reduces radiation-induced skin reactions in C3H mice  

SciTech Connect

To determine (a) whether a wound dressing gel that contains acemannan extracted from aloe leaves affects the severity of radiation-induced acute skin reactions in C3H mice; (b) if so, whether other commercially available gels such as a personal lubricating jelly and a healing ointment have similar effects; and (c) when the wound dressing gel should be applied for maximum effect. Male C3H mice received graded single doses of gamma radiation ranging from 30 to 47.5 Gy to the right leg. In most experiments, the gel was applied daily beginning immediately after irradiation. Dose-response curves were obtained by plotting the percentage of mice that reached or exceeded a given peak skin reaction as a function of dose. Curves were fitted by logit analysis and ED{sub 50} values, and 95% confidence limits were obtained. The average peak skin reactions of the wound dressing gel-treated mice were lower than those of the untreated mice at all radiation doses tested. The ED{sub 50} values for skin reactions of 2.0-2.75 were approximately 7 Gy higher in the wound dressing gel-treated mice. The average peak skin reactions and the ED{sub 50} values for mice treated with personal lubricating jelly or healing ointment were similar to irradiated control values. Reduction in the percentage of mice with skin reactions of 2.5 or more was greatest in the groups that received wound dressing gel for at least 2 weeks beginning immediately after irradiation. There was no effect if gel was applied only before irradiation or beginning 1 week after irradiation. Wound dressing gel, but not personal lubricating jelly or healing ointment, reduces acute radiation-induced skin reactions in C3H mice if applied daily for at least 2 weeks beginning immediately after irradiation. 31 refs., 4 figs., 1 tab.

Roberts, D.B.; Travis, E.L. [Univ. of Texas M.D. Anderson Cancer Center, Houston, TX (United States)] [Univ. of Texas M.D. Anderson Cancer Center, Houston, TX (United States)

1995-07-15

274

Characterization of potential elastase inhibitor-peptides regulated by a molecular switch for wound dressings applications.  

PubMed

Elastase plays an important role in wound healing process, degrading damaged tissue and allowing complete tissue recovery. The levels of human neutrophil elastase (HNE) are usually controlled by endogenous inhibitors. However, in the presence of high levels of elastase, like the ones present in chronic wounds, the inhibitors cannot overcome this overproduction and the enzyme starts to degrade the surrounding healthy tissue. In this work we report the development of a molecular switch to control the elastase activity in the exudate of non-healing chronic wounds. A peptide library was generated and screened in a microarray format for protein kinase-mediated phosphorylation. Two peptides were identified as casein kinase I? (CKI) substrates: KRCCPDTCGIKCL and its analogous peptide KRMMPDTMGIKML, with cysteine residues replaced by methionine residues. These peptides were studied in solution, both in the phosphorylated and non-phosphorylated forms as potential inhibitors for elastase. The obtained results show that the reversible process of phosphorylation/dephosphorylation results in differential inhibitory activity of the peptides. Thus the reversible process of phosphorylation/dephosphorylation can be used as a kind of molecular switch to control elastase activity. Degradation studies reveal that both the inhibitor-peptides and CKI are degraded by elastase. These results envisage the safe utilisation of these inhibitor-peptides together with CKI in the formulation of wound dressings. PMID:22226196

Barros, Sandra Cerqueira; Martins, José Alberto; Marcos, João Carlos; Cavaco-Paulo, Artur

2012-02-10

275

Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study  

PubMed Central

In this study, a mixture of poly(vinyl alcohol) (PVA) and chitosan oligosaccharides (COS) was electrospun with silver nanoparticles (AgNPs) to produce fibrous mats for use in wound healing. The AgNPs were reduced by COS prior to electrospinning or Ag+ was reduced via ultraviolet irradiation in nanofibers. The morphologies of the PVA/COS/AgNO3 and PVA/COS-AgNP nanofibers were analyzed by scanning electron microscopy. Formation of the AgNPs was investigated by field emission transmission electron microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. We also evaluated the biocompatibility of the nanofibers, particularly their cytotoxicity to human skin fibroblasts and potential to cause primary skin irritation. The in vitro antibacterial activity and in vivo wound healing capacity of the nanofibers were also investigated. The nanofibers had a smooth surface with an average diameter of 130–192 nm. The diameters of the AgNPs were in the range of 15–22 nm. The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria. PVA/COS-AgNP nanofibers accelerated the rate of wound healing over that of the control (gauze). The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

Li, Chenwen; Fu, Ruoqiu; Yu, Caiping; Li, Zhuoheng; Guan, Haiyan; Hu, Daqiang; Zhao, Dehua; Lu, Laichun

2013-01-01

276

Assessment of reinforced poly(ethylene glycol) chitosan hydrogels as dressings in a mouse skin wound defect model.  

PubMed

Wound dressings of chitosan are biocompatible, biodegradable, antibacterial and hemostatic biomaterials. However, applications for chitosan are limited due to its poor mechanical properties. Here, we conducted an in vivo mouse angiogenesis study on reinforced poly(ethylene glycol) (PEG)-chitosan (RPC) hydrogels. RPC hydrogels were formed by cross-linking chitosan with PEGs of different molecular weights at various PEG to chitosan ratios in our previous paper. These dressings can keep the wound moist, had good gas exchange capacity, and was capable of absorbing or removing the wound exudate. We examined the ability of these RPC hydrogels and neat chitosan to heal small cuts and full-thickness skin defects on the backs of male Balb/c mice. Histological examination revealed that chitosan suppressed the infiltration of inflammatory cells and accelerated fibroblast proliferation, while PEG enhanced epithelial migration. The RPC hydrogels promoted wound healing in the small cuts and full layer wounds. The optimal RPC hydrogel had a swelling ratio of 100% and a water vapor transmission rate (WVTR) of about 2000 g/m(2)/day. In addition, they possess good mechanical property and appropriate degradation rates. Thus, the optimal RPC hydrogel formulation functioned effectively as a wound dressing and promoted wound healing. PMID:23623072

Chen, Szu-Hsien; Tsao, Ching-Ting; Chang, Chih-Hao; Lai, Yi-Ting; Wu, Ming-Fung; Chuang, Ching-Nan; Chou, Hung-Chia; Wang, Chih-Kuang; Hsieh, Kuo-Haung

2013-07-01

277

Novel copper (II) alginate hydrogels and their potential for use as anti-bacterial wound dressings.  

PubMed

The incorporation of a metal ion, with antimicrobial activity, into an alginate dressing is an attractive approach to minimize infection in a wound. In this work, copper (II) cross-linked alginate hydrogels were successfully prepared using a two-step cross-linking procedure. In the first step, solid alginate films were prepared using a solvent-casting method from soft gels of alginate solutions that had been lightly cross-linked using a copper (II) (Cu(2+)) sulfate solution. In the second step, the films were further cross-linked in a corresponding Cu(2+) sulfate solution using a dipping method to further improve their dimensional stability. Alginate solution (at 2%w/v) and Cu(2+) sulfate solution (at 2%w/v) in acetate buffer at a low pH provided soft films with excellent swelling behavior. An increase in either Cu(2+) ion concentration or cross-linking time led to hydrogels with more densely-cross-linked networks that limited water absorption. The hydrogels clearly showed antibacterial activity against Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis and Streptococcus pyogenes, which was proportional to the Cu(2+) ion concentration. Blood coagulation studies showed that the tested copper (II) cross-linked alginate hydrogels had a tendency to coagulate fibrin, and possibly had an effect on pro-thrombotic coagulation and platelet activation. Conclusively, the prepared films are likely candidates as antibacterial wound dressings. PMID:25029588

Klinkajon, Wimonwan; Supaphol, Pitt

2014-08-01

278

GNPs-CS/KGM as Hemostatic First Aid Wound Dressing with Antibiotic Effect: In Vitro and In Vivo Study  

PubMed Central

Ideal wound dressing materials should create a good healing environment, with immediate hemostatic effects and antimicrobial activity. In this study, chitosan/konjac glucomannan (CS/KGM) films embedded with gentamicin-loaded poly(dex-GMA/AAc) nanoparticles (giving GNP-CS/KGM films) were prepared as novel wound dressings. The results revealed that the modified CS/KGM films could be used as effective wound dressings and had significant hemostatic effects. With their microporous structure, the films could effectively absorb water from blood and trap blood cells. The gentamicinloaded poly(dex-GMA/AAc) nanoparticles (GNPs) also further promoted blood clotting, with their favorable water uptake capacity. Thus, the GNP-CS/KGM films had wound healing and synergistic effects that helped to stop bleeding from injuries, and also showed good antibiotic abilities by addition of gentamicin to the NPs. These GNPCS/KGM films can be considered as promising novel biodegradable and biocompatible wound dressings with hemostatic capabilities and antibiotic effects for treatment of external bleeding injuries.

Qiao, Youbei; Li, Fei; Li, Wei; Wu, Hong; Ren, Bo

2013-01-01

279

A retrospective analysis of ambulatory burn patients: focus on wound dressings and healing times  

PubMed Central

INTRODUCTION In this study, we retrospectively analysed healing times of ambulatory burn patients after silver-based dressings were introduced in late December 2005, and compared the results with those obtained before. PATIENTS AND METHODS Data were collected in November–December 2005 and in January–February 2006. We excluded from the study: (i) admitted patients; (ii) patients with mixed superficial partial thickness and deep partial thickness burns; (iii) patients with full-thickness burns; and (iv) operated patients that came for follow-up. We recorded the age, sex, cause (flame vs scald), burn depth, dressings used and healing times. RESULTS We selected 347 patients corresponding to 455 burned areas (64.4% superficial and 35.6% deep; 47.7% treated in 2005 and 52.3% in 2006). During the years 2005 and 2006, there was an increase in the use of silver-based dressings (2005, 9.7%; 2006, 38.7%; chi-squared test, P < 0.001) and a decrease in the use of paraffin gauzes (2005, 66.4%; 2006, 40.3%; chi-squared test, P < 0.001). The healing time of overall burns and of superficial burns showed no significant differences between 2005 and 2006. However, in deep partial thickness burns, a significant reduction was present (2006, 19; 2005, 29 days; Student's t-test, P < 0.01). Among all dressings, paraffin gauzes had the shortest healing times in superficial burns (5 days); with silver-based dressings in deep burns, the healing times were nanocrystalline silver (16 days) and silver carboxymethylcellulose (21 days). CONCLUSIONS Results of our retrospective study would suggest that paraffin gauzes are a valuable option in superficial burns, while silver-based dressings are preferable in deep burns.

Gravante, Gianpiero; Montone, Antonio

2010-01-01

280

Evaluation of a Wound Dressing Composed of Hyaluronic Acid and Collagen Sponge Containing Epidermal Growth Factor in Diabetic Mice  

Microsoft Academic Search

This study investigated the effect of a wound dressing composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF) on wound healing in diabetic mice. High-molecular-weight (HMW) HA aqueous solution, hydrolyzed low-molecular-weight (LMW) HA aqueous solution and heat-denatured Col aqueous solution were mixed, followed by freeze-drying to obtain a spongy sheet. Cross-linkage between Col molecules was

Shinya Kondo; Hayato Niiyama; Akane Yu; Yoshimitsu Kuroyanagi

2012-01-01

281

Gentamicin-Loaded Wound Dressing With Polyvinyl Alcohol\\/Dextran Hydrogel: Gel Characterization and In Vivo Healing Evaluation  

Microsoft Academic Search

To develop a gentamicin-loaded wound dressing, cross-linked hydrogel films were prepared with polyvinyl alcohol (PVA) and\\u000a dextran using the freezing–thawing method. Their gel properties such as gel fraction, swelling, water vapor transmission test,\\u000a morphology, tensile strength, and thermal property were investigated. In vitro protein adsorption test, in vivo wound healing test, and histopathology were performed. Dextran decreased the gel fraction,

Ma-Ro Hwang; Jong Oh Kim; Jeong Hoon Lee; Yong Il Kim; Jeong Hoon Kim; Sun Woo Chang; Sung Gju Jin; Jung Ae Kim; Won Seok Lyoo; Sung Soo Han; Sae Kwang Ku; Chul Soon Yong; Han-Gon Choi

2010-01-01

282

Accelerated Epithelization Under a Highly Vapor-Permeable Wound Dressing Is Associated with Increased Precipitation of Fibrin(ogen) and Fibronectin  

Microsoft Academic Search

In a previous study we showed that the use of a newly developed, highly water vapor permeable, PEU wound dressing accelerates the epithelization of partial-thickness wounds more than an occlusive wound dressing (OpSite) in comparison with air exposure. The purpose of this study was to investigate the distribution of fibrin(ogen), fibronectin, and type IV collagen during the epithelization process under

Marcel F. Jonkman; Eduard A. Hoeksma; Paul Nieuwenhuis

1990-01-01

283

Bacterial cellulose and bacterial cellulose-chitosan membranes for wound dressing applications.  

PubMed

Bacterial cellulose (BC) and bacterial cellulose-chitosan (BC-Ch) membranes were successfully produced in large scale. BC was synthesized by Acetobacter xylinum. BC-Ch was prepared by immersing BC in chitosan followed by freeze-drying. The surface morphology of BC and BC-Ch membranes were examined by a scanning electron microscope (SEM). SEM images showed that BC-Ch possessed a denser fibril network with smaller pores than BC. Infrared spectroscopy was used to confirm the incorporation of chitosan in BC-Ch. The swelling behavior, water retention capacity, and mechanical properties of BC and BC-Ch were further evaluated. Results indicated that both membranes maintained proper moisture contents for an extensive period without dehydration. The tensile strength and elongation at break for BC-Ch were slightly lower while the Young's modulus was higher. Cell culture studies demonstrated that BC and BC-Ch had no cytotoxicity. In the antibacterial test, the addition of chitosan in BC showed significant growth inhibition against Escherichia coli and Staphylococcus aureus. The effects of BC and BC-Ch on skin wound healing were assessed by rat models. Histological examinations revealed that wounds treated with BC-Ch epithelialized and regenerated faster than those treated with BC or Tegaderm. Therefore, BC-Ch was considered as a potential candidate for wound dressing materials. PMID:23544580

Lin, Wen-Chun; Lien, Chun-Chieh; Yeh, Hsiu-Jen; Yu, Chao-Ming; Hsu, Shan-Hui

2013-04-15

284

Occlusive Dressings and the Healing of Standardized Abrasions  

PubMed Central

Context: Acute skin trauma during sport participation, resulting in partial-thickness abrasions, is common. The limited investigations focusing on the acute wound environment and dressing techniques and the subsequent lack of evidence-based standards complicate clinical wound care decisions. Objective: To examine the effects of occlusive dressings on healing of standardized, partial-thickness abrasions. Design: Controlled, counterbalanced, repeated-measures design. Setting: University laboratory. Patients or Other Participants: Sixteen healthy women (n ?=? 10) and men (n ?=? 6). Intervention(s): Four standardized, partial-thickness abrasions were inflicted. Film, hydrogel, and hydrocolloid occlusive dressings and no dressing (control) were applied. Participants returned on postwound days 1, 3, 5, 7, 10, and 14 for digital imaging. Wound healing time was measured by change in wound contraction (cm2) and change in wound color (chromatic red) and luminance in red, green, and blue color values. Main Outcome Measure(s): Wound contraction, color (chromatic red), and luminance. Results: A day-by-dressing interaction was found for wound contraction, color, and luminance. Post hoc testing indicated that the film and hydrocolloid dressings produced greater wound contraction than the hydrogel and no dressing on days 7 and 10. Film, hydrogel, and hydrocolloid dressings also resulted in greater wound contraction than the control on day 14. Hydrocolloid dressings produced smaller measures of color and greater measures of luminance than no dressing on day 7. Film, hydrogel, and hydrocolloid dressings also resulted in smaller measures of color and greater measures of luminance compared with no dressing on days 10 and 14. Conclusions: When compared with the control (no dressing), the film, hydrogel, and hydrocolloid occlusive dressings were associated with a faster healing rate of partial-thickness abrasions across time measured by wound contraction, color, and luminance. Overall, these data indicate that occlusive dressings were more effective in healing than no dressing was.

Beam, Joel W

2008-01-01

285

Easily Applied Long Lasting Dressing Based on Poroplastic (Trade Name) Materials. Phase IIB. Dual-Drug Dressing Incorporating Clindamycin with Gentamicin.  

National Technical Information Service (NTIS)

Gentamicin-loaded Poroplastic membrane wound dressing of defined composition have been furnished to USAIDR, for in vivo evaluation. They have been shown to be effective for control of wound infections in vivo and their sustained release properties were fo...

S. J. Davidson S. Obermayer D. Nichols N. Hurd B. Allen

1984-01-01

286

Electrospinning of carboxyethyl chitosan/poly(vinyl alcohol)/silk fibroin nanoparticles for wound dressings.  

PubMed

Composite nanofibrous membranes of water-soluble N-carboxyethyl chitosan/poly(vinyl alcohol)/silk fibroin nanoparticles were successfully fabricated by electrospinning. The composite nanofibers were subjected to detailed analysis by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetry (DSC). SEM results investigated that the morphology and diameter of the nanofibers were affected by silk fibroin nanoparticles content. XRD and DSC demonstrated that there was intermolecular hydrogen bonding among the molecules of carboxyethyl chitosan, silk fibroin and PVA. The crystalline microstructure of the electrospun fibers was not well developed. The indirect cytotoxicity assessments of the nanofibers were studied. The result showed the nanofibers had good biocompatibility. This novel electrospun matrix would be used as potential wound dressing for skin regeneration. PMID:23164753

Zhou, Yingshan; Yang, Hongjun; Liu, Xin; Mao, Jun; Gu, Shaojin; Xu, Weilin

2013-02-01

287

Irradiation of polyvinyl alcohol and polyvinyl pyrrolidone blended hydrogel for wound dressing  

NASA Astrophysics Data System (ADS)

Polyvinyl alcohol and polyvinyl pyrrolidone (PVA-PVP) blended hydrogel for wound dressing has been prepared by using gamma rays irradiation technique. The gel fraction, mechanical properties, the water content and water absorption performance of the hydrogels were measured. It was found that the gel fraction increases with increasing irradiation dose but never reaches 100% of gel. The PVA/PVP blended hydrogel has a water content in the range between 60% and 80% and water absorption between 40% and 250%. The water vapor transmission rate value (WVTR) of the PVA/PVP blended hydrogel varies between 50 and 200 g/m 2/h. The hydrogel could be considered as good barrier against microbes. According to in vitro assessment it was found that the PVA/PVP blended hydrogel was very useful material that can meet the efficacy requirement and its healing rate was comparable with sterilized gauze and sofratulle.

Razzak, Mirzan T.; Darwis, Darmawan; Zainuddin; Sukirno

2001-07-01

288

Electrospun chitosan/sericin composite nanofibers with antibacterial property as potential wound dressings.  

PubMed

Chitosan and sericin are natural and low cost biomaterials. Both biomaterials displayed good compatibility to human tissues and antibacterial properties for biomedical application. In this study, we have successfully fabricated chitosan/sericin composite nanofibers by electrospinning. The obtained composite nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA) studies. The composite nanofibers had good morphology with diameter between 240nm and 380nm. In vitro methyl thiazolyl tetrazolium (MTT) assays demonstrated that the chitosan/sericin composite nanofibers were biocompatible and could promote the cell proliferation. Furthermore, the composite nanofibers showed good bactericidal activity against both of Gram-positive and Gram-negative bacteria. Thus, the chitosan/sericin composite nanofibers are promising for wound dressing applications. PMID:24769088

Zhao, Rui; Li, Xiang; Sun, Bolun; Zhang, Ying; Zhang, Dawei; Tang, Zhaohui; Chen, Xuesi; Wang, Ce

2014-07-01

289

Reduced cellular toxicity of a new silver-containing antimicrobial dressing and clinical performance in non-healing wounds.  

PubMed

Bacterial colonisation of wounds may delay wound healing. Modern silver-containing dressings are antimicrobial, yet cellular toxicity is a serious side-effect. We provide data for a newly formulated silver-containing ointment dressing, Atrauman Ag, for antimicrobial activity and cytotoxicity. Atrauman Ag effectively killed a panel of commensal skin as well as pathogenic bacterial strains while cytotoxicity for HaCaT keratinocytes was only around 10%. With these favourable in vitro tests, Atrauman Ag was analysed in 86 patients with traumatic and non-healing wounds of different aetiologies. The wound state was evaluated for 3 subsequent dressing changes. The slough score was reduced from 59.2 to 35.8%, granulation tissue increased from 27 to 40% and epithelialisation went up from 12.1 to 24%. We conclude that Atrauman Ag has a superior profile of antimicrobial activity over cellular toxicity and the low silver ion release rate may prevent interference with wound-healing mechanisms. PMID:16612141

Ziegler, K; Görl, R; Effing, J; Ellermann, J; Mappes, M; Otten, S; Kapp, H; Zoellner, P; Spaeth, D; Smola, H

2006-01-01

290

Curcumin Loaded Poly(?-Caprolactone) Nanofibers: Diabetic Wound Dressing with Antioxidant and Anti-inflammatory Properties  

PubMed Central

1. Curcumin is a naturally occurring poly-phenolic compound with a broad range of favorable biological functions including anti-cancer, anti-oxidant and anti-inflammatory activities. The low bioavailability and in vivo stability of curcumin require the development of suitable carrier vehicles to deliver the molecule in a sustained manner at therapeutic levels. 2. We investigated the feasibility and potential of poly(caprolactone) (PCL) nanofibers as a delivery vehicle for curcumin for wound healing applications. By optimizing the electrospinning parameters, bead-free curcumin loaded PCL nanofibers were developed. 3. The fibers showed sustained release of curcumin for 72 h and could be made to deliver a dosage much lower than the reported cytotoxic concentration while remaining bioactive. Human foreskin fibroblast cells (HFF-1) showed more than 70% viability on curcumin loaded nanofibers. The antioxidant activity of curcumin loaded nanofibers was demonstrated using an ORAC assay and by the ability of the fibers to maintain the viability of HFF-1 cells on the fibers under a condition of oxidative stress. The curcumin loaded nanofibers also reduced inflammatory induction as evidenced by low levels of IL-6 release from mouse monocyte-macrophages seeded on the fibers following stimulation by E.coli-derived lipopolysaccharide (LPS). The in vivo wound healing capability of the curcumin loaded PCL nanofibers was demonstrated by an increased rate of wound closure in a streptozotocin (STZ) induced diabetic mice model. 4. These results demonstrate that curcumin loaded PCL nanofiber matrix is bioactive and has potential as a wound dressing with antioxidant and anti-inflammatory properties.

Merrell, Jonathan G.; McLaughlin, Shaun W.; Tie, Lu; Laurencin, Cato T.; Chen, Alex F.; Nair, Lakshmi S.

2009-01-01

291

Development of salmon milt DNA/salmon collagen composite for wound dressing.  

PubMed

This study aims to develop a novel wound dressing comprising salmon milt DNA (sDNA) and salmon collagen (SC). The sDNA/SC composites were prepared by incubating a mixture of an acidic SC solution, an sDNA solution, and a collagen fibrillogenesis inducing buffer (pH 6.8) containing a crosslinking agent (water-soluble carbodiimide) for gelation, and a subsequent ventilation-drying process to give sDNA/SC films. The conjugation between sDNA and SC were confirmed by sDNA-elution assay and fluorescence microscopy. The sDNA/SC films with various doses of sDNA (sDNA/SC weight ratios of 1:5, 1:10, and 1:20) were used for in vitro cell cultures to evaluate their growth potentials of normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK). It was found that NHDF proliferation was increased by sDNA conjugation, whereas NHEK proliferation was dose-dependently inhibited. In light of the in vitro results, the appropriate dose of sDNA for in vivo study was determined to be the ratio of 1:10. For the implantation in full-thickness skin defects in rat dorsal region, the sDNA/SC films were reinforced by incorporating them on a porous SC sponge, because the sDNA/SC films exhibited early contraction and inadequate morphologic stability when implanted in vivo. The regenerated tissue in the sDNA/SC sponge group showed similar morphology to native dermis, while the SC sponge group without sDNA showed epithelial overgrowth, indicating that additional sDNA could reduce epidermal overgrowth. Furthermore, blood capillary formation was significantly enhanced in the sDNA/SC sponge group when compared to the SC sponge group. In conclusion, the results suggest that the sDNA/SC composite could be a potential wound dressing for clinical applications. PMID:18592347

Shen, XuanRi; Nagai, Nobuhiro; Murata, Masaru; Nishimura, Daisuke; Sugi, Masahito; Munekata, Masanobu

2008-12-01

292

Wound bed preparation 2014 update: management of critical colonization with a gentian violet and methylene blue absorbent antibacterial dressing and elevated levels of matrix metalloproteases with an ovine collagen extracellular matrix dressing.  

PubMed

Wound bed preparation (WBP) is a paradigm for holistic patient care that includes treatment of the cause along with patient-centered concerns before optimizing the components of local wound care (debridement, infection/inflammation, moisture balance, and, when required, the edge effect). This review incorporates a methylene blue and gentian violet bound foam dressing for critical colonization and an ovine collagen extracellular matrix dressing for reduction of elevated levels of matrix metalloproteases into the WBP paradigm. PMID:24521847

Sibbald, R Gary; Ovington, Liza G; Ayello, Elizabeth A; Goodman, Laurie; Elliott, James A

2014-03-01

293

Leg or foot amputation - dressing change  

MedlinePLUS

... clean wash cloths to clean and dry your wound Adaptic dressing. This is a dressing that does not stick ... clean scissors. Gently remove the dressing from the wound. If the dressing is stuck, wet it with warm tap water, ...

294

Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications.  

PubMed

Chitosan, a naturally occurring polysaccharide with abundant resources, has been extensively exploited for various biomedical applications, typically as wound dressings owing to its unique biocompatibility, good biodegradability and excellent antibacterial properties. In this work, composite nanofibrous membranes of chitosan (CS) and silk fibroin (SF) were successfully fabricated by electrospinning. The morphology of electrospun blend nanofibers was observed by scanning electron microscopy (SEM) and the fiber diameters decreased with the increasing percentage of chitosan. Further, the mechanical test illustrated that the addition of silk fibroin enhanced the mechanical properties of CS/SF nanofibers. The antibacterial activities against Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) were evaluated by the turbidity measurement method; and results suggest that the antibacterial effect of composite nanofibers varied on the type of bacteria. Furthermore, the biocompatibility of murine fibroblast on as-prepared nanofibrous membranes was investigated by hematoxylin and eosin (H&E) staining and MTT assays in vitro, and the membranes were found to promote the cell attachment and proliferation. These results suggest that as-prepared chitosan/silk fibroin (CS/SF) composite nanofibrous membranes could be a promising candidate for wound healing applications. PMID:20957110

Cai, Zeng-Xiao; Mo, Xiu-Mei; Zhang, Kui-Hua; Fan, Lin-Peng; Yin, An-Lin; He, Chuang-Long; Wang, Hong-Sheng

2010-01-01

295

Comparision of Vacuum-Asisted Closure and Moist Wound Dressing in the Treatment of Diabetic Foot Ulcers  

PubMed Central

Background: Vacuum-assisted closure (VAC) is a new method in wound care which speeds wound healing by causing vacuum, improving tissue perfusion and suctioning the exudates. This study aims to evaluate its efficacy in the treatment of diabetic foot ulcers. Materials and Methods: Thirteen patients with diabetic foot ulcers were enrolled in the moist dressing group, and 10 patients in the VAC group. The site, size and depth of the wound were inspected and recorded before and every three days during the study period. Patient satisfaction and formation of granulation tissue were also assessed. Results: Improvement of the wound in the form of reducing the diameter and depth and increasing proliferation of granulation tissue was significant in most of the patients of the VAC group after two weeks. Satisfaction of patients in the VAC group was evaluated as excellent as no amputation was done in this group. Wagner score was reduced in both the study groups, although this decrement was not significant in the moist dressing group. Conclusion: VAC appears to be as safe as and more efficacious than moist dressing for the treatment of diabetic foot ulcers.

Ravari, Hassan; Modaghegh, Mohammad-Hadi Saeed; Kazemzadeh, Gholam Hosein; Johari, Hamed Ghoddusi; Vatanchi, Attieh Mohammadzadeh; Sangaki, Abolghasem; Shahrodi, Mohammad Vahedian

2013-01-01

296

Dual-functional Polyurea Microcapsules for Chronic Wound Care Dressings: Sustained Drug Delivery and Non-leaching Infection Control  

NASA Astrophysics Data System (ADS)

A new design of dual-functional polyurea microcapsules was proposed for chronic wound dressings to provide both non-leaching infection control and sustained topical drug delivery functionalities. Quaternary ammonium functionalized polyurea microcapsules (MCQs) were synthesized under mild conditions through an interfacial crosslinking reaction between branched polyethylenimine (PEI) and 2,4-toluene diisocyanate (TDI) in a dimethylformamide/cyclohexane emulsion. An in-situ modification method was developed to endow non-leaching surface antimicrobial properties to MCQs via bonding antimicrobial surfactants to surface isocyanate residues on the polyurea shells. The resultant robust MCQs with both non-leaching antimicrobial properties and sustained drug releasing properties have potential applications in medical textiles, such as chronic wound dressings, for infection control and drug delivery.

He, Wei

297

A berberine-loaded electrospun poly-(epsilon-caprolactone) nanofibrous membrane with hemostatic potential and antimicrobial property for wound dressing.  

PubMed

In this study, a berberine-loaded electrospun poly-(epsilon-caprolactone) nanofibrous membrane (B-NFM) was formulated. The B-NFM was composed of uniform nanofibers with the average diameter of 190 +/- 53 nm. Whole blood assay revealed that the B-NFM had higher absorption capacity than gauze wound dressing (GWD) and non-drug-loaded nanofibrous membrane (NFM). Hemostatic studies showed that the B-NFM was more efficient at promoting blood clotting than GWD and NFM. B-NFM was also demonstrated to possess broad-spectrum antimicrobial activity. Biocompatibility studies indicated that B-NFM had good compatibility with skin fibroblast cells. These results suggested that the B-NFM can promote in vitro hemostasis to minimize wound bleeding, absorb biofluid to provide a favorable environment, inhibit on-site microbial agents to prevent wound infection, and support skin cell growth to facilitate wound healing. These data highly suggest a potential clinical use of B-NFM as wound dressing material. PMID:23909131

Bao, Jing; Yang, Bo; Sun, Yuyu; Zu, Youli; Deng, Ying

2013-07-01

298

The use of moist wound-healing dressings in the management of split-thickness skin graft donor sites: a systematic review.  

PubMed

The aim of this systematic review was to determine the best available evidence related to the post-harvest management of split-thickness skin graft (STSG) donor sites. Studies included in the review were those involving patients of any age examining interventions relating to the post-harvest management of STSG donors and were intra-individual or randomized controlled trials. All studies were checked for methodological quality, and data were extracted using a data extraction tool. Many studies were combined in meta-analysis. The present report concerns studies examining moist and non-moist wound-healing dressings. Broad comparisons of moist wound-healing dressings against traditional non-moist dressings favoured moist wound-healing approaches in terms of healing rates, pain and infection. In comparing dressings within and between moist wound-healing dressing groups, the lack of studies of sufficient quality prevented determining a 'best dressing' for STSG donors. Moist wound-healing products have distinct clinical advantages over non-moist products in the management of STSG donors. There is a strong case for further head-to-head studies comparing products within the moist wound-healing group. PMID:12694482

Wiechula, Rick

2003-04-01

299

Design and evaluation of drug-loaded wound dressing having thermoresponsive, adhesive, absorptive and easy peeling properties  

Microsoft Academic Search

In order to develop a novel unique wound dressing, a combination of self-adhesive Eudragit E film with antibacterial drug-loaded poly (N-isopropyl-acrylamide) (PNIPAAm) microgel beads was designed. The result indicates that the tack property of Eudragit E film increased with an increase of the PNIPAAm microgel beads added, but there was no significant difference between the dried PNIPAAm microgel beads with

Shan-Yang Lin; Ko-Shao Chen; Liang Run-Chu

2001-01-01

300

Fabrication of a novel poly(3-hydroxyoctanoate) / nanoscale bioactive glass composite film with potential as a multifunctional wound dressing  

NASA Astrophysics Data System (ADS)

Fabrication of a composite scaffold of nanobioglass (n-BG) 45S5 and poly(3-hydroxyocatnoate), P(3HO) was studied for the first time with the aim of developing a novel, multifunctional wound dressing. The incorporation of n-BG accelerated blood clotting time and its incorporation in the polymer matrix enhanced the wettability, surface roughness and bio-compatibility of the scaffold.

Rai, Ranjana; Boccaccini, Aldo R.; Knowles, Jonathan C.; Locke, Ian C.; Gordge, Michael P.; McCormick, Aine; Salih, Vehid; Mordon, Nicola; Keshavarz, Tajalli; Roy, Ipsita

2010-06-01

301

Possibility of wound dressing using poly( l-leucine)\\/poly(ethylene glycol)\\/poly( l-leucine) triblock copolymer  

Microsoft Academic Search

ABA-type block copolymers (abbreviated as LEL) composed of poly(l-leucine) (PLL) as the A component and poly(ethylene glycol) (PEG) as the B component were synthesized by ring-opening polymerization of l-leucine N-carboxyanhydride initiated by primary amino group located at both ends of PEG chain. A silver sulfadiazine (AgSD)-impregnated wound dressing of sponge type was prepared by the lyophilization method. Morphological structure of

Hyun-Jung Kim; Eun-Young Choi; Jong-Suk Oh; Hyun-Chul Lee; Sung-Sik Park; Chong-Su Cho

2000-01-01

302

Impact of adhesive surgical tape and wound dressings on the skin, with reference to skin stripping.  

PubMed

Age- and disease-related factors can cause the skin to become susceptible to damage, particularly at dressing change. This review recommends ways of avoiding skin trauma when removing adhesive dressings and surgical tapes. PMID:18494433

Cutting, K F

2008-04-01

303

An in-vitro study of the pressure-relieving properties of four wound dressings for foot ulcers.  

PubMed

This in-vitro study reports on a selection of mechanical tests on four wound dressings: Allevyn (Smith and Nephew), Biatain (Coloplast), Lyofoam (Seton Scholl) and Tielle (Johnson and Johnson). The aim of the study was to investigate these dressings in the laboratory by subjecting them to dry and wet compression tests, shearing tests and a cyclical test. As predicted, all dressings performed differently in the range of tests, with Lyofoam deforming the most when subjected to compression testing (5.6 mm). During wet compression, differences in the physical properties of all the materials tested were identified. During shearing testin, Allevyn and Tielle withstood the greatest shear deflection before failure, but Biatain withstood the greatest force before failing. During the cyclical testing, Lyofoam only reduced by 1% of its original thickness, while Biatain reduced by 30%. No one dressing performed better in all the tests. However, as an all-round 'pressure-relieving' dressing, Allevyn consistently outperformed the others in the study. PMID:12964226

Ashford, R L; Freear, N D; Shippen, J M

2001-02-01

304

A prospective, open-label study to assess the clinical performance of a foam dressing in the management of chronic wounds.  

PubMed

Polyurethane foam dressings are used to help absorb wound exudate while maintaining a moist wound environment. To assess the clinical performance of a foam dressing, a prospective, open-label evaluation of 57 outpatients (mean age 69 years) with delayed/non-healing wounds (one per patient, average duration 1.4 +/- 4.1 years) of different etiologies was conducted in 15 medical centers in Germany. Physicians assessed the clinical appearance of the wound bed, periwound skin, and exudate level at the beginning and final evaluation and dressing performance characteristics were rated. Patients were asked to assess their wound pain as none, mild, moderate, or strong. After three dressing changes (performed approximately 3 days apart), slough and necrotic tissue on the wound surface changed from an average of 54% (+/- 33%) to 29% (+/- 25%). At baseline, granulation tissue covered an average of 41% (+/- 31%) of the wound bed compared to 59% (+/- 24%) at the final assessment and the proportion of wounds assessed as heavily exudating decreased from 32% to 11%. During the evaluation, the proportion of patients without periwound skin problems increased from 49% to 66% and the percentage of patients with wound pain decreased from 45% to 26%. Between 80% and 90% of the participating physicians rated the product attributes assessed as "very good" or "good." PMID:16687768

Zoellner, Petra; Kapp, Holger; Smola, Hans

2006-05-01

305

Mechanical properties and in vitro characterization of polyvinyl alcohol-nano-silver hydrogel wound dressings.  

PubMed

Polyvinyl alcohol (PVA) hydrogels are materials for potential use in burn healing. Silver nanoparticles can be synthesized within PVA hydrogels giving antimicrobial hydrogels. Hydrogels have to be swollen prior to their application, and the common medium available for that in hospitals is saline solution, but the hydrogel could also take up some of the wound's fluid. This work developed gamma-irradiated PVA/nano-Ag hydrogels for potential use in burn dressing applications. Silver nitrate (AgNO3) was used as nano-Ag precursor agent. Saline solution, phosphate-buffered solution (PBS) pH 7.4 and solution pH 4.0 were used as swelling media. Microstructural evaluation revealed an effect of the nanoparticles on PVA crystallization. The swelling of the PVA-Ag samples in solution pH 4.0 was low, as was their silver delivery, compared with the equivalent samples swollen in the other media. The highest swelling and silver delivery were related to samples prepared with 0.50% AgNO3, and they also presented lower strength in PBS pH 7.4 and solution pH 4.0. Both PVA-Ag samples were also non-toxic and presented antimicrobial activity, confirming that 0.25% AgNO3 concentration is sufficient to establish an antimicrobial effect. Both PVA-Ag samples presented suitable mechanical and swelling properties in all media, representative of potential burn site conditions. PMID:24501677

Oliveira, R N; Rouzé, R; Quilty, B; Alves, G G; Soares, G D A; Thiré, R M S M; McGuinness, G B

2014-02-01

306

[Preparation and clinical application of polyvinyl alcohol/drug-loaded chitosan microsphere composite wound dressing].  

PubMed

In order to prepare and apply the polyvinyl alcohol/drug-loaded chitosan microspheres composite wound dressing, we first prepared chitosan microspheres by emulsion cross-linking method, and then added chitosan microspheres into the reactants during the acetalization of polyvinyl alcohol and formaldehyde. We further studied the morphology, water absorption, swelling degree, mechanical properties and in vitro release of the sponge with different amount of chitosan microspheres. The results showed that polyvinyl alcohol/drug-loaded chitosan composite sponge has porous structure with connectionism. Increasing the amount of chitosan microspheres would make the apertures smaller, so that the water absorption and the swelling of sponge decreased, but the tensile strength and compressive strength increased. With the increase of the amount of chitosan microspheres, the drug absorption of cefradine and the release rate increase, and the release time become longer. With the results of toxicity grade of 0 to 1, this type of composite sponge is non-toxic and meets the requirement of biocompatibility. The observation of rabbit nasal cavity after surgical operation suggested that polyvinyl acetal sponge modified with the chitosan has antiphlogistic, hemostatic and non-adherent characteristic, and can promote the healing and recovering of the nasalmucosa. After using this composite material, best growing surroundings for patients' granulation tissue were provided. Exposed bone and tendon were covered well with granulation tissue. PMID:21604506

Zhang, Xiuju; Lin, Zhidan; Chen, Wenbin; Song, Ying; Li, Zhizhong

2011-04-01

307

Sericin-carboxymethyl cellulose porous matrices as cellular wound dressing material.  

PubMed

In this study, porous three-dimensional (3D) hydrogel matrices are fabricated composed of silk cocoon protein sericin of non-mulberry silkworm Antheraea mylitta and carboxymethyl cellulose. The matrices are prepared via freeze-drying technique followed by dual cross-linking with glutaraldehyde and aluminum chloride. The microstructure of the hydrogel matrices is assessed using scanning electron microscopy and biophysical characterization are carried out using Fourier transform infrared spectroscopy and X-ray diffraction. The transforming growth factor ?1 release from the cross-linked matrices as a growth factor is evaluated by immunosorbent assay. Live dead assay and 3-[4,5-dimethylthiazolyl-2]-2,5-diphenyl tetrazolium bromide assay show no cytotoxicity of blended matrices toward human keratinocytes. The matrices support the cell attachment and proliferation of human keratinocytes as observed through scanning electron microscope and confocal images. Gelatin zymography demonstrates the low levels of matrix metalloproteinase 2 (MMP-2) and insignificant amount of MMP-9 in the culture media of cell seeded matrices. Low inflammatory response of the matrices is indicated through tumor necrosis factor alpha release assay. The results indicate that the fabricated matrices constitute 3D cell-interactive environment for tissue engineering applications and its potential use as a future cellular biological wound dressing material. PMID:23853114

Nayak, Sunita; Kundu, S C

2014-06-01

308

Synthesis and characterization of poly(vinyl alcohol) membranes with quaternary ammonium groups for wound dressing.  

PubMed

2-[(acryloyloxy)ethyl]Trimethylammonium chloride (AETMAC) was grafted onto poly(vinyl alcohol) (PVA) using ceric ammonium nitrate (CAN) as a redox initiator. A series of graft co-polymer (PVA-g-PAETMAC) membranes with different contents of AETMAC were prepared with a casting method. The incorporation of AETMAC into PVA chains was confirmed by element analysis and Fourier transform infrared spectroscopy. The effects of grafting on the thermal properties, water take, water vapor transmission rate (WVTR), contact angle, antibacterial activity and cytotoxicity of PVA-g-PAETMAC membranes were investigated. The experiment results showed that PVA-g-PAETMAC membrane has a higher equilibrium swelling ratio, surface hydrophilicity and WVTR than pure PVA membrane. Moreover, the higher the content of AETMAC, the higher were equilibrium swelling ratio, surface hydrophilicity and WVTR. In vitro bacterial adhesion study demonstrated a significantly reduced number of Staphylococcus aureus and Escherichia coli on PVA-g-PAETMAC surfaces when compared to PVA surface. In addition, no significant difference in the in vitro cytotoxicity was observed between PVA and PVA-g-PAETMAC membranes. The presence of quaternary ammonium groups did not reduce L929 cell growth. Therefore, the PVA-g-PAETMAC membranes have the potential for wound-dressing application. PMID:20233501

Chen, Kuo-Yu; Lin, Yu-Sheng; Yao, Chun-Hsu; Li, Ming-Hsien; Lin, Jui-Che

2010-01-01

309

Chitosan and alginate polyelectrolyte complex membranes and their properties for wound dressing application.  

PubMed

This study investigated the characteristics and drug release properties of membranes of chitosan and alginate prepared via a casting/solvent evaporation technique. Membranes of chitosan and alginate with silver sulfadiazine as model drug incorporated in different concentrations and different membrane compositions were obtained. The polyblend solution viscosity reached to the highest at the composition polyblends of (1:1). This chitosan/alginate membranes showed pH- and ionic strength-dependent water uptake properties and had the WVTR rang from 442 to 618 g/m(2)/day. The maximum value of the dry membrane of breaking strength was 52.16 MPa and the maximum value of the wet membrane breaking elongation was 46.28%. The results of controlled release studies showed that the silver sulfadiazine release rate was the fastest when the alginate content was 50%. On the basis of the requisite physical properties, the chitosan-alginate PEC membrane can be considered for potential wound dressing or controlled release application. PMID:20101440

Meng, Xin; Tian, Feng; Yang, Jian; He, Chun-Nian; Xing, Nan; Li, Fan

2010-05-01

310

Loofah sponge as an interface dressing material in negative pressure wound therapy: results of an in vivo study.  

PubMed

Since the introduction of negative pressure wound therapy (NPWT), the physiological effects of various interface dressing materials have been studied. The purpose of this experimental study was to compare the use of loofah sponge to standard polyurethane foam or a cotton gauze sponge. Three wounds, each measuring 3 cm x 3 cm, were created by full-thickness skin excision on the dorsal sides of 24 New Zealand adult white rabbits. The rabbits were randomly divided into four groups of six rabbits each. In group 1 (control), conventional saline-moistened gauze dressing was provided and changed at daily intervals. The remaining groups were provided NPWT dressings at -125 mm Hg continuous pressure. This dressing was changed every 3 days for 9 days; group 2 was provided polyurethane foam, group 3 had conventional saline-soaked antimicrobial gauze, and group 4 had loofah sponge. Wound area measurements and histological findings (inflammation, granulation tissue, neovascularization, and reepithelialization) were analyzed on days 3, 6, and 9. Wound area measurements at these intervals were significantly different between the control group and study groups (P<0.05). Granulation and neovascularization scores were also significantly different between the control and treatment groups at day 3 (P=0.002). No differences in any of the healing variables studied were observed between the other three dressing materials. According to scanning electron microscopy analysis of the three interface materials, the mean pore size diameter of foam and gauze interface materials was 415.80±217.58 ?m and 912.33±116.88 ?m, respectively. The pore architecture of foam was much more regular than that of gauze. The average pore size diameter of loofah sponge was 736.83±23.01 ?m; pores were hierarchically located--ie, the smaller ones were usually peripheral and larger ones werecentral. For this study, the central part of loofah sponge was discarded to achieve a more homogenous structure of interface material. Loofah sponge study results were similar to those using gauze or foam, but the purchase price of loofah sponge is lower than that of currently available interface dressings. More experimental, randomized controlled studies are needed to confirm these results. PMID:24610559

Tuncel, Umut; Turan, Aydin; Markoc, Fatma; Erkorkmaz, Unal; Elmas, Cigdem; Kostakoglu, Naci

2014-03-01

311

Comparison of the antioxidant properties of wound dressing materials–carboxymethylcellulose, hyaluronan benzyl ester and hyaluronan, towards polymorphonuclear leukocyte-derived reactive oxygen species  

Microsoft Academic Search

In chronic wounds, factors are released which perpetuate inflammatory processes, including polymorphonuclear leukocyte (PMN)-derived reactive oxygen species (ROS), such as superoxide radical (O2?) and hydroxyl radical (OH) species. The glycosaminoglycan, hyaluronan, has established antioxidant properties towards ROS, although the antioxidant potential of wound dressing biomaterials, such as 75% benzyl esterified hyaluronan (BEHA) and carboxymethylcellulose (CMCH), are less characterised. This study

R. Moseley; M. Walker; R. J. Waddington; W. Y. J. Chen

2003-01-01

312

Biologic Dressings.  

National Technical Information Service (NTIS)

Cutaneous biologic dressings have been useful and versatile in wound management of severely burned patients. Availability, relative costs, patient acceptability, and similarities to human skin have made porcine skin a satisfactory skin substitute. Porcine...

J. M. Shuck O. H. Payne

1983-01-01

313

Recent Developments in Topical Wound Therapy: Impact of Antimicrobiological Changes and Rebalancing the Wound Milieu  

PubMed Central

Wound therapy improves every year by developing new wound treatment options or by advancing already existing wound materials, for example, adding self-releasing analgesic drugs or growth factors to wound dressings, or by binding and inactivating excessive proteases. Also new dressing materials based on silk fibers and enhanced methods to reduce bacterial burden, for example, cold argon plasma, might help to fasten wound healing.

Erfurt-Berge, Cornelia; Renner, Regina

2014-01-01

314

Pain and quality of life for patients with venous leg ulcers: proof of concept of the efficacy of BiatainR-Ibu, a new pain reducing wound dressing  

Microsoft Academic Search

Wound pain is a serious problem for elderly patients suffering from chronic leg ulcers, and it may lead to reduced wound healing rates and reduced quality of life. Biatains-Ibu Non-adhesive (Coloplast A\\/S), a new pain-reducing moist wound healing dressing containing ibuprofen was tested for pain reduction, safety, and efficacy on 1012 patients in a single-blinded crossover study against Biatain Non-adhesive

Bo Jorgensen; Gitte Juel Friis; Finn Gottrup

2006-01-01

315

Effect of chitosan-gluconic acid conjugate/poly(vinyl alcohol) cryogels as wound dressing on partial-thickness wounds in diabetic rats.  

PubMed

We previously developed chitosan cryogels from chitosan-gluconic acid conjugate without using toxic additives for wound care. In this study, we improved physiological characteristics of the previous cryogels by incorporating poly(vinyl alcohol) that also form cryogels. Mechanical strength of the cryogels was more than two times higher than that of the previous cryogels. Furthermore, the incorporation of poly(vinyl alcohol) enhanced water retention and resistance to degradation of the gels by lysozyme. The cryogels retained the favorable biological properties of the previous cryogels that they accelerate infiltration of inflammatory cells into wound sites. Time period for repairing 50 % of initial area of partial-thickness skin wound treated with the cryogels (4.0 ± 1.1 days) was shorter than those with gauze (6.5 ± 0.3 days) or a commercial hydrogel dressing (5.7 ± 0.3 days). Finally, we confirmed that incorporation of basic fibroblast growth factor into the cryogels was effective to further accelerate wound healing (2.7 ± 1.0 days). These results demonstrate that the cryogels in this study are promising for wound care. PMID:23801501

Takei, Takayuki; Nakahara, Hideki; Tanaka, Sadao; Nishimata, Hiroto; Yoshida, Masahiro; Kawakami, Koei

2013-10-01

316

Evaluation of Human Amniotic Membrane as a Wound Dressing for Split-Thickness Skin-Graft Donor Sites  

PubMed Central

Human amniotic membrane (HAM) has been used as a biomaterial in various surgical procedures and exceeds some qualities of common materials. We evaluated HAM as wound dressing for split-thickness skin-graft (STSG) donor sites in a swine model (Part A) and a clinical trial (Part B). Part A: STSG donor sites in 4 piglets were treated with HAM or a clinically used conventional polyurethane (PU) foil (n = 8 each). Biopsies were taken on days 5, 7, 10, 20, 40, and 60 and investigated immunohistochemically for alpha-smooth muscle actin (?SMA: wound contraction marker), von Willebrand factor (vWF: angiogenesis), Ki-67 (cell proliferation), and laminin (basement membrane integrity). Part B: STSG donor sites in 45 adult patients (16 female/29 male) were treated with HAM covered by PU foam, solely by PU foam, or PU foil/paraffin gauze (n = 15 each). Part A revealed no difference in the rate of wound closure between groups. HAM showed improved esthetic results and inhibitory effects on cicatrization. Angioneogenesis was reduced, and basement membrane formation was accelerated in HAM group. Part B: no difference in re-epithelialization/infection rate was found. HAM caused less ichor exudation and less pruritus. HAM has no relevant advantage over conventional dressings but might be a cost-effective alternative.

Loeffelbein, Denys J.; Rohleder, Nils H.; Eddicks, Matthias; Baumann, Claudia M.; Stoeckelhuber, Mechthild; Wolff, Klaus-D.; Drecoll, Enken; Steinstraesser, Lars; Hennerbichler, Simone; Kesting, Marco R.

2014-01-01

317

Evaluation of human amniotic membrane as a wound dressing for split-thickness skin-graft donor sites.  

PubMed

Human amniotic membrane (HAM) has been used as a biomaterial in various surgical procedures and exceeds some qualities of common materials. We evaluated HAM as wound dressing for split-thickness skin-graft (STSG) donor sites in a swine model (Part A) and a clinical trial (Part B). Part A: STSG donor sites in 4 piglets were treated with HAM or a clinically used conventional polyurethane (PU) foil (n = 8 each). Biopsies were taken on days 5, 7, 10, 20, 40, and 60 and investigated immunohistochemically for alpha-smooth muscle actin (?SMA: wound contraction marker), von Willebrand factor (vWF: angiogenesis), Ki-67 (cell proliferation), and laminin (basement membrane integrity). Part B: STSG donor sites in 45 adult patients (16 female/29 male) were treated with HAM covered by PU foam, solely by PU foam, or PU foil/paraffin gauze (n = 15 each). Part A revealed no difference in the rate of wound closure between groups. HAM showed improved esthetic results and inhibitory effects on cicatrization. Angioneogenesis was reduced, and basement membrane formation was accelerated in HAM group. Part B: no difference in re-epithelialization/infection rate was found. HAM caused less ichor exudation and less pruritus. HAM has no relevant advantage over conventional dressings but might be a cost-effective alternative. PMID:25003117

Loeffelbein, Denys J; Rohleder, Nils H; Eddicks, Matthias; Baumann, Claudia M; Stoeckelhuber, Mechthild; Wolff, Klaus-D; Drecoll, Enken; Steinstraesser, Lars; Hennerbichler, Simone; Kesting, Marco R

2014-01-01

318

Investigations on preparation and properties of modified polyacrylamide hydrogels for application as wound dressing materials.  

PubMed

Investigations on the influence of the polymerization conditions and feed ratio as well as various modification of the composite polyacrylamide-agar hydrogels on their properties has been presented. Results of the model studies have been utilized in the optimalization of the manufacturing parameters and procedures of the preparation of hydrogel dressing foils and granulated dressing gels. PMID:7479424

Lukaszczyk, J

1995-01-01

319

Randomized clinical trial of the effect of semi-occlusive dressings on the microflora and clinical outcome of acute facial wounds.  

PubMed

This study investigated whether treatment with occlusive, hydroactive polyurethane dressings affects the microflora and clinical outcome of acute wounds. A randomized, controlled trial was performed on 60 patients with acute facial lacerations. Following primary closure, patients were provided with either a dry-gauze dressing or a hydroactive polyurethane dressing (Cutinova Thin). The wounds were assessed clinically and microbiologically prior to closure, then after 5, 28 and 56 days. The dressing, which was removed at day 5, was also sent for microbiological culture. Wounds treated with the polyurethane dressing showed improved comfort and contour (p < 0.04), less erythema (p < 0.03) and less potential for scarring (p < 0.01) at day 5. At day 28 and day 56, there were no significant differences in the clinical assessment between the test and control groups (p > 0.05). A total of 518 isolates were recovered from the patients during the study. Mean number of isolates and bacterial growth density were calculated in both groups. A similar range of microorganisms were obtained from both treatment groups with no clear difference in organism colonization. This study shows the potential usefulness of facial wounds as a human model for studying acute wound healing responses and anti-scarring therapy. While short-term, clinical benefits of occlusive dressings were evident in the management of acute wounds, these data clearly show that all studies of scarring following acute wounding should extend over at least 3 months post-injury to allow for spontaneous improvement to occur. PMID:11013016

Thomas, D W; Hill, C M; Lewis, M A; Stephens, P; Walker, R; Von Der Weth, A

2000-01-01

320

Scars and Wounds  

MedlinePLUS

... hands well before and after changing a wound dressing. Never re-use dressings. Always keep the wound clean. Unless you were ... gauze. If possible, use paper tape. Keep your dressing clean and dry. If it gets wet or ...

321

The preparation of polyurethane foam combined with pH-sensitive alginate\\/bentonite hydrogel for wound dressings  

Microsoft Academic Search

Polyurethane (PU) foam was combined with protein drug-loaded pH-sensitive alginate-bentonite hydrogel for wound dressings.\\u000a Alginate is a linear copolymer composed of 1–4-linked ?-D-mannuronic acid (M) and its c-5-epimer ?-Lguluronic acid (G). The amount of (M) and (G) and their sequential distribution are varied depending on the alginate source.\\u000a Soluble sodium alginate can become a hydrogel when cross-linked with divalent cations

Seung-Taek Oh; Woo-Ram Kim; Sung-Hoon Kim; Yong-Chan Chung; Jong-Shin Park

2011-01-01

322

Electrospinning of poly(vinyl pyrrolidone)–iodine complex and poly(ethylene oxide)\\/poly(vinyl pyrrolidone)–iodine complex – a prospective route to antimicrobial wound dressing materials  

Microsoft Academic Search

New nanofibers containing poly(vinyl pyrrolidone)–iodine complex (PVP–iodine) were obtained by electrospinning in order to prepare materials suitable for wound dressings. Different approaches were used: a one-step method based on electrospinning of PVP–iodine or poly(ethylene oxide)\\/PVP–iodine solutions and a three-step method based on electrospinning of PVP or poly(ethylene oxide)\\/PVP mixed solutions followed by photo-mediated crosslinking of the obtained nanofibers and subsequent

Milena Ignatova; Nevena Manolova; Iliya Rashkov

2007-01-01

323

Negative pressure wound therapy with Bio-Dome dressing technology in the treatment of complex wounds: A case series.  

PubMed

The treatment of complex wounds is difficult and not always effective. Various treatment options are used with varying degrees of success. Negative pressure wound therapy (NPWT) is a cost-efficient and effective way to help treat these wounds. The use of a vacuum device applies the negative pressure to the site of the wound and promotes waste removal and increases circulation and tissue formation. While various NPWT systems are currently on the market, we utilised the ConvaTec Engenex® system with Bio-DomeTM technology; however, our case study is not intended to advocate the specific use of this system, but instead focusses on the use of NPWT as a viable option for wound healing. Each of the following case study patients presented with difficult-to-heal wounds that failed traditional therapeutic approaches. Through the use of NPWT, our patients saw major wound size reductions. Each patient exhibited at least a 94% reduction in wound area, wound volume or both. PMID:24762555

L Penny, H; Spinazzola, J; Green, A; Rifkah, M; Faretta, M; Youshaw, D; Weaver, A; Zaki, P

2014-04-17

324

In situ forming antibacterial dextran blend hydrogel for wound dressing: SAA technology vs. spray drying.  

PubMed

This study focuses on designing microparticulate carriers based on high-mannuronic alginate and amidated pectin blend loaded with gentamicin sulphate able to move rapidly from dry to soft hydrogel. Supercritical assisted atomization was used to produce microparticles in form of dry powder and characteristics were compared with those obtained by spray-drying. Particles with very high encapsulation efficiency (approximately 100%) and small diameter (less than 2 ?m) showed good flowability and high fluid uptake enabling wound site filling and limiting bacterial proliferation. Moisture transmission of the in situ formed hydrogel was about 95 g/m(2)h, ideal to avoid wound dehydration or occlusion phenomena. All formulations presented a burst effect, suitable to prevent infection spreading at the beginning of the therapy, followed by prolonged release (4-10 days) related to drug/polymers ratio. Antimicrobial tests showed stronger effect than pure GS over time (up-to 24 days) and the ability to degrade preformed biofilms, essential to properly treat infected wounds. PMID:24299894

De Cicco, Felicetta; Reverchon, Ernesto; Adami, Renata; Auriemma, Giulia; Russo, Paola; Calabrese, Elena C; Porta, Amalia; Aquino, Rita P; Del Gaudio, Pasquale

2014-01-30

325

Sterculia crosslinked PVA and PVA-poly(AAm) hydrogel wound dressings for slow drug delivery: mechanical, mucoadhesive, biocompatible and permeability properties.  

PubMed

The present study deals with the synthesis and characterization of sterculia crosslinked PVA and PVA-AAm hydrogel wound dressings. The hydrogels have been characterized by SEMs, FTIR, TGA and swelling studies. This article also discusses comparison of swelling, drug release and biomedical properties such as blood compatibility, antimicrobial activity, mucoadhesion, tensile strength, burst strength, water vapour permeability, oxygen diffusion and microbial penetration of both hydrogel wound dressings. These polymeric films have absorbed 4.80 ± 0.15 and 6.32 ± 0.15 gram/g of gel of simulated wound fluid respectively and swelling occurred through Case II diffusion mechanism. The release of antibiotic drugs occurred through non-Fickian and Case II diffusion mechanisms, respectively. These polymeric films have been observed to be permeable for oxygen and water vapour but have shown impermeability to the micro-organism. Sterculia-PVA hydrogel wound dressing has shown more blood compatibility as compared to the other film. All these results indicate that these hydrogel films may be used as wound dressings for the slow release of antibiotic drug to the wound. PMID:22498279

Singh, Baljit; Pal, Lok

2012-05-01

326

Thermo-responsive wound dressings by grafting chitosan and poly(N-isopropylacrylamide) to plasma-induced graft polymerization modified non-woven fabrics  

NASA Astrophysics Data System (ADS)

To obtain a chitosan wound dressings with temperature-responsive characteristics, polypropylene (PP) non-woven fabric (NWF) was modified by direct current pulsed oxygen plasma-induced grafting polymerization of acrylic acid (AAc) to improve hydrophilicity and to introduce carboxylic acid groups. Conjugation of chitosan and poly(N-isopropylacrylamide) (PNIPAAm) followed by using water-soluble carbodiimide as a coupling agent to form a novel bigraft PP-g-chitosan-g-PNIPAAm wound dressing. The amount of chitosan and PNIPAAm grafted to PP-g-chitosan-g-PNIPAAm were 83.0 ± 4.6 ?g/cm2 and 189.5 ± 8.2 ?g/cm2, respectively. The surface chemical composition and microstructure of the NWF were studied by electron spectroscopy for chemical analysis (ESCA) and scanning electron microscopy (SEM). The linkages between AAc, chitosan, and PNIPAAm were confirmed with the formation of amide bonds. Physical properties of the NWF were characterized and potentials of these NWFs as wound dressings were evaluated using SD rat as the animal model. NWFs contained PNIPAAm were better than those contained only chitosan in wound healing rates and the wound areas covered by PP-g-chitosan-g-PNIPAAm wound dressings healed completely in 17 days.

Chen, Jyh-Ping; Kuo, Chang-Yi; Lee, Wen-Li

2012-12-01

327

Biocompatibility Evaluation of a New Hydrogel Dressing Based on Polyvinylpyrrolidone/Polyethylene Glycol  

PubMed Central

The composition of the dressings is based on polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), and agar. The electron beam irradiation technique has been used to prepare hydrogel wound dressings. The in vitro biocompatibility of the hydrogel was investigated by check samples (hydrocolloid Comfeel), antibacterial test (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia Coli k12), anti fungal test (Candida Albicans) and cytotoxicity test (Fibroblast L929). Results have shown cell attachment characteristics and nontoxicity of all samples. Antibacterial testing also showed that the antibacterial effect of the hydrogel sample to the check sample increased to 30%. Also, investigation of antifungal analysis did not show any trace of fungi growth on the surface of the hydrogel, whereas antifungal effect did not observe on the surface of the check sample. Finally, this hydrogel sample showed a good in vitro biocompatibility.

Biazar, Esmaeil; Roveimiab, Ziba; Shahhosseini, Gholamreza; Khataminezhad, Mohammadreza; Zafari, Mandana; Majdi, Ali

2012-01-01

328

Efficacy of polarized hydroxyapatite and silk fibroin composite dressing gel on epidermal recovery from full-thickness skin wounds.  

PubMed

Hydroxyapatite (HA) has been widely used to produce biomaterials. We reported that electrically polarized HA (pHA) induces cells as a scaffold. Recently, in the biomedical field, many studies are underway, seeking new applications of silk fibroin (SF), because SF can be gelatinized and still retain its biocompatibility and permeability. To develop an innovative composite material for effective wound dressings, we added pHA powder to SF and gelatinized the compound. We then applied the gel composite on full-thickness porcine skin wounds for investigation of its healing effect. The pHA transforms the SF structure into a porous three-dimensional scaffold. It was found that the SF gel containing pHA (pHA/SF) had higher promotive effects on wound healing, re-epithelization, and matrix formation than did the other prepared gel composites in the study. The pHA/SF effectively advanced the maturation of fibroblast cells benefiting from its structural advantages and stored charges. To our knowledge, this is the first report of the preparation of pHA/SF gel and identification of its wound-healing effects in vivo. PMID:19213051

Okabayashi, Rumi; Nakamura, Miho; Okabayashi, Tamaki; Tanaka, Yumi; Nagai, Akiko; Yamashita, Kimihiro

2009-08-01

329

Collagen-Based Films Containing Liposome-Loaded Usnic Acid as Dressing for Dermal Burn Healing  

PubMed Central

The aim of this study was assess the effect of collagen-based films containing usnic acid as a wound dressing for dermal burn healing. Second-degree burn wounds were performed in forty-five Wistar rats, assigned into nine groups: COL—animals treated with collagen-based films; PHO—animals treated with collagen films containing empty liposomes; UAL—animals treated with collagen-based films containing usnic acid incorporated into liposomes. After 7, 14, and 21 days the animals were euthanized. On 7th day there was a moderate infiltration of neutrophils, in UAL, distributed throughout the burn wounds, whereas in COL and PHO, the severity of the reaction was slighter and still limited to the margins of the burn wounds. On the 14th day, the inflammatory reaction was less intense in UAL, with remarkable plasma cells infiltration. On the 21st day, there was reduction of the inflammation, which was predominantly composed of plasma cells in all groups, particularly in UAL. The use of the usnic acid provided more rapid substitution of type-III for type-I collagen on the 14th day, and improved the collagenization density on the 21st day. It was concluded that the use of reconstituted bovine type-I collagen-based films containing usnic acid improved burn healing process in rats.

Nunes, Paula S.; Albuquerque-Junior, Ricardo L. C.; Cavalcante, Danielle R. R.; Dantas, Marx D. M.; Cardoso, Juliana C.; Bezerra, Marilia S.; Souza, Jamille C. C.; Serafini, Mairim Russo; Quitans-Jr, Lucindo J.; Bonjardim, Leonardo R.; Araujo, Adriano A. S.

2011-01-01

330

Experiences with biosynthetic dressings.  

PubMed

Almost all documented clinical experience shows that occlusively dressed wounds heal more quickly and with less pain, tenderness, and swelling than undressed wounds. Clinical experience with occlusive skin dressings on acute and chronic wounds, on diseased skin, and on normal skin is reviewed. This report presents the effects of some of the newest oxygen-permeable and oxygen-nonpermeable occlusive dressings: three polyurethane adhesive films (Bioclusive, Op-Site, and Tegaderm), a polyethylene oxide hydrogel dressing (Vigilon), and a hydroactive polymer dressing (DuoDerm). PMID:3973143

Eaglstein, W H

1985-02-01

331

Development of novel chitin\\/nanosilver composite scaffolds for wound dressing applications  

Microsoft Academic Search

Antibiotic resistance of microorganisms is one of the major problems faced in the field of wound care and management resulting\\u000a in complications like infection and delayed wound healing. Currently a lot of research is focused on developing newer antimicrobials\\u000a to treat wounds infected with antibiotic resistant microorganisms. Silver has been used as an antimicrobial agent for a long\\u000a time in

K. Madhumathi; P. T. Sudheesh Kumar; S. Abhilash; V. Sreeja; H. Tamura; K. Manzoor; S. V. Nair; R. Jayakumar

2010-01-01

332

Comparative effectiveness of a bilayered living cellular construct and a porcine collagen wound dressing in the treatment of venous leg ulcers.  

PubMed

Using data from a national wound-specific electronic medical record (WoundExpert, Net Health, Pittsburgh, PA), we compared the effectiveness of a bilayered living cellular construct (BLCC) and an acellular porcine small intestine submucosa collagen dressing (SIS) for the treatment of venous leg ulcer. Data from 1,489 patients with 1,801 refractory venous leg ulcers (as defined by failure to have >40% reduction in size in the 4 weeks prior to treatment) with surface areas between 1 and 150?cm(2) in size, treated between July 2009 and July 2012 at 158 wound care facilities across the US were analyzed. Patient baseline demographics and wound characteristics were comparable between groups. Kaplan-Meier-derived estimates of wound closure for BLCC (1,451 wounds) was significantly greater (p?=?0.01, log-rank test) by weeks 12 (31% vs. 26%), 24 (50% vs. 41%), and 36 (61% vs. 46%), respectively, compared with SIS (350 wounds). BLCC treatment reduced the median time to wound closure by 44%, achieving healing 19 weeks sooner (24 vs. 43 weeks, p?=?0.01, log-rank test). Treatment with BLCC increased the probability of healing by 29% compared with porcine SIS dressing (hazard ratio?=?1.29 [95% confidence interval 1.06, 1.56], p?=?0.01). PMID:24628712

Marston, William A; Sabolinski, Michael L; Parsons, Nathan B; Kirsner, Robert S

2014-05-01

333

In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing.  

PubMed

Bacterial cellulose has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity, which is one of critical skin-barrier functions in wound healing. To overcome such deficiency, we developed a novel method to synthesize and impregnate silver nanoparticles on to bacterial cellulose nanofibres (AgNP-BC). Uniform spherical silver nano-particles (10-30 nm) were generated and self-assembled on the surface of BC nano-fibers, forming a stable and evenly distributed Ag nanoparticles coated BC nanofiber. Such hybrid nanostructure prevented Ag nanoparticles from dropping off BC network and thus minimized the toxicity of nanoparticles. Regardless the slow Ag(+) release, AgNP-BC still exhibited significant antibacterial activities with more than 99% reductions in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Moreover, AgNP-BC allowed attachment and growth of epidermal cells with no cytotoxicity emerged. The results demonstrated that AgNP-BC could reduce inflammation and promote wound healing. PMID:24507345

Wu, Jian; Zheng, Yudong; Song, Wenhui; Luan, Jiabin; Wen, Xiaoxiao; Wu, Zhigu; Chen, Xiaohua; Wang, Qi; Guo, Shaolin

2014-02-15

334

A Case Report of the Use of Nanocrystalline Silver Dressing in the Management of Acute Surgical Site Wound Infected With MRSA to Prevent Cutaneous Necrosis Following Revision Surgery  

Microsoft Academic Search

The authors report the use of nanocrystalline silver (Acticoat 7, Smith and Nephew, London, UK) in an acute surgical wound to prevent localized skin necrosis due to infection, thereby avoiding skin grafting as a secondary procedure. Two patients were successfully treated with Acticoat 7 dressings without using systemic antimicrobials after developing methicillin-resistant Staphylococcus aureus infection in the surgical site. Despite

Mayukh Bhattacharyya; Helen Bradley

2008-01-01

335

Systematic review of the use of honey as a wound dressing  

Microsoft Academic Search

OBJECTIVE: To investigate topical honey in superficial burns and wounds though a systematic review of randomised controlled trials. DATA SOURCES: Cochrane Library, MEDLINE, EMBASE, PubMed, reference lists and databases were used to seek randomised controlled trials. Seven randomised trials involved superficial burns, partial thickness burns, moderate to severe burns that included full thickness injury, and infected postoperative wounds. REVIEW METHODS:

Owen A Moore; Lesley A Smith; Fiona Campbell; Kate Seers; Henry J McQuay; R Andrew Moore

2001-01-01

336

Characterization of potential Elastase Inhibitor-Peptides regulated by a molecular switch for wound dressings applications  

Microsoft Academic Search

Elastase plays an important role in wound healing process, degrading damaged tissue and allowing complete tissue recovery. The levels of human neutrophil elastase (HNE) are usually controlled by endogenous inhibitors. However, in the presence of high levels of elastase, like the ones present in chronic wounds, the inhibitors can not overcome this overproduction and the enzyme starts to degrade the

Sandra Cerqueira Barros; José Alberto Martins; João Carlos Marcos; Artur Cavaco-Paulo

337

Influence of selected wound dressings on PMN elastase in chronic wound fluid and their antioxidative potential in vitro  

Microsoft Academic Search

Exudates from non-healing wounds contain elevated levels of proteolytic enzymes, like elastase from polymorphonuclear granulocytes (PMN elastase), reactive oxygen species (ROS) and reactive nitrogen species (RNS). The overproduction of proteolytic enzymes leads to reduced concentrations of growth factors and proteinase inhibitors, resulting in an imbalance between degradation and remodelling processes. Thus, the reduction of protein-degrading enzymes and scavenging of ROS

Ute Schönfelder; Martin Abel; Cornelia Wiegand; Dieter Klemm; Peter Elsner; Uta-Christina Hipler

2005-01-01

338

A note on an in vitro test system to compare the bactericidal properties of wound dressings.  

PubMed

An in vitro method of comparing the antimicrobial properties of dressings used on human skin is described and has been tested with two samples of tulle gras. The method has advantages over agar diffusion methods by providing flexibility of test conditions and kinetic data for analysis. PMID:3928570

Holland, K T; Davis, W

1985-07-01

339

Development of alginate wound dressings linked with hybrid peptides derived from laminin and elastin  

Microsoft Academic Search

We designed hybrid peptides, SIRVXVXPG (X: A or G), from a laminin-derived peptide, SIKVAV, and an elastin-derived peptide, VGVAPG, and tried to develop new alginate dressings linked covalently with the hybrid peptides. First, we examined the effectiveness of the hybrid peptides for cell attachment and proliferation using normal human dermal fibroblasts (NHDF) in vitro. The hybrid peptides promoted attachment of

Tadashi Hashimoto; Yoshihisa Suzuki; Masao Tanihara; Yoshimi Kakimaru; Kyoko Suzuki

2004-01-01

340

Wound-dressing materials with antibacterial activity from electrospun polyurethane-dextran nanofiber mats containing ciprofloxacin HCl.  

PubMed

Dextran is a versatile biomacromolecule for preparing electrospun nanofibrous membranes by blending with either water-soluble bioactive agents or hydrophobic biodegradable polymers for biomedical applications. In this study, an antibacterial electrospun scaffold was prepared by electrospinning of a solution composed of dextran, polyurethane (PU) and ciprofloxacin HCl (CipHCl) drug. The obtained nanofiber mats have good morphology. The mats were characterized by various analytical techniques. The interaction parameters between fibroblasts and the PU-dextran and PU-dextran-drug scaffolds such as viability, proliferation, and attachment were investigated. The results indicated that the cells interacted favorably with the scaffolds especially the drug-containing one. Moreover, the composite mat showed good bactericidal activity against both of Gram-positive and Gram-negative bacteria. Overall, our results conclude that the introduced scaffold might be an ideal biomaterial for wound dressing applications. PMID:22944448

Unnithan, Afeesh R; Barakat, Nasser A M; Pichiah, P B Tirupathi; Gnanasekaran, Gopalsamy; Nirmala, R; Cha, Youn-Soo; Jung, Che-Hun; El-Newehy, Mohamed; Kim, Hak Yong

2012-11-01

341

All-natural composite wound dressing films of essential oils encapsulated in sodium alginate with antimicrobial properties.  

PubMed

We present natural polymeric composite films made of essential oils (EOs) dispersed in sodium alginate (NaAlg) matrix, with remarkable anti-microbial and anti-fungal properties. Namely, elicriso italic, chamomile blue, cinnamon, lavender, tea tree, peppermint, eucalyptus, lemongrass and lemon oils were encapsulated in the films as potential active substances. Glycerol was used to induce plasticity and surfactants were added to improve the dispersion of EOs in the NaAlg matrix. The topography, chemical composition, mechanical properties, and humidity resistance of the films are presented analytically. Antimicrobial tests were conducted on films containing different percentages of EOs against Escherichia coli bacteria and Candida albicans fungi, and the films were characterized as effective or not. Such diverse types of essential oil-fortified alginate films can find many applications mainly as disposable wound dressings but also in food packaging, medical device protection and disinfection, and indoor air quality improvement materials, to name a few. PMID:24211443

Liakos, Ioannis; Rizzello, Loris; Scurr, David J; Pompa, Pier Paolo; Bayer, Ilker S; Athanassiou, Athanassia

2014-03-25

342

Curcumin-loaded poly(epsilon-caprolactone) nanofibres: diabetic wound dressing with anti-oxidant and anti-inflammatory properties.  

PubMed

1. Curcumin is a naturally occurring poly-phenolic compound with a broad range of favourable biological functions, including anti-cancer, anti-oxidant and anti-inflammatory activities. The low bioavailability and in vivo stability of curcumin require the development of suitable carrier vehicles to deliver the molecule in a sustained manner at therapeutic levels. 2. In the present study, we investigated the feasibility and potential of poly(caprolactone) (PCL) nanofibres as a delivery vehicle for curcumin for wound healing applications. By optimizing the electrospinning parameters, bead-free curcumin-loaded PCL nanofibres were developed. 3. The fibres showed sustained release of curcumin for 72 h and could be made to deliver a dose much lower than the reported cytotoxic concentration while remaining bioactive. Human foreskin fibroblast cells (HFF-1) showed more than 70% viability on curcumin-loaded nanofibres. 4. The anti-oxidant activity of curcumin-loaded nanofibres was demonstrated using an oxygen radical absorbance capacity (ORAC) assay and by the ability of the fibres to maintain the viability of HFF-1 cells under conditions of oxidative stress. 5. The curcumin-loaded nanofibres also reduced inflammatory induction, as evidenced by low levels of interleukin-6 release from mouse monocyte-macrophages seeded onto the fibres following stimulation by Escherichia coli-derived lipopolysaccharide. 6. The in vivo wound healing capability of the curcumin loaded PCL nanofibres was demonstrated by an increased rate of wound closure in a streptozotocin-induced diabetic mice model. 7. These results demonstrate that the curcumin-loaded PCL nanofibre matrix is bioactive and has potential as a wound dressing with anti-oxidant and anti-inflammatory properties. PMID:19473187

Merrell, Jonathan G; McLaughlin, Shaun W; Tie, Lu; Laurencin, Cato T; Chen, Alex F; Nair, Lakshmi S

2009-12-01

343

Chitosan-cellulose composite for wound dressing material. Part 2. Antimicrobial activity, blood absorption ability, and biocompatibility.  

PubMed

Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects. In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL?+?CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-? (TNF-?) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts. The [CEL?+?CS] composites were found to inhibit the growth of both Gram positive and negative micro-organisms. For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively. The composites are nontoxic to fibroblasts; that is, fibroblasts, which are critical to the formation of connective tissue matrix were found to grow and proliferate in the presence of the composites. They effectively absorb blood, and at the same rate and volume as commercially available wound dressings. The composites, in both air-dried and lyophilized forms, significantly inhibit the production of TNF-? and IL-6 by stimulated macrophages. These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL?+?CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1199-1206, 2014. PMID:24407857

Harkins, April L; Duri, Simon; Kloth, Luther C; Tran, Chieu D

2014-08-01

344

Systematic review of the use of honey as a wound dressing  

PubMed Central

Objective To investigate topical honey in superficial burns and wounds though a systematic review of randomised controlled trials. Data sources Cochrane Library, MEDLINE, EMBASE, PubMed, reference lists and databases were used to seek randomised controlled trials. Seven randomised trials involved superficial burns, partial thickness burns, moderate to severe burns that included full thickness injury, and infected postoperative wounds. Review methods Studies were randomised trials using honey, published papers, with a comparator. Main outcomes were relative benefit and number-needed-to-treat to prevent an outcome relating to wound healing time or infection rate. Results One study in infected postoperative wounds compared honey with antiseptics plus systemic antibiotics. The number needed to treat with honey for good wound healing compared with antiseptic was 2.9 (95% confidence interval 1.7 to 9.7). Five studies in patients with partial thickness or superficial burns involved less than 40% of the body surface. Comparators were polyurethane film, amniotic membrane, potato peel and silver sulphadiazine. The number needed to treat for seven days with honey to produce one patient with a healed burn was 2.6 (2.1 to 3.4) compared with any other treatment and 2.7 (2.0 to 4.1) compared with potato and amniotic membrane. For some or all outcomes honey was superior to all these treatments. Time for healing was significantly shorter for honey than all these treatments. The quality of studies was low. Conclusion Confidence in a conclusion that honey is a useful treatment for superficial wounds or burns is low. There is biological plausibility.

Moore, Owen A; Smith, Lesley A; Campbell, Fiona; Seers, Kate; McQuay, Henry J; Moore, R Andrew

2001-01-01

345

Electrospun antibacterial polyurethane-cellulose acetate-zein composite mats for wound dressing.  

PubMed

In this study, an antibacterial electrospun nanofibrous scaffolds with diameters around 400-700 nm were prepared by physically blending polyurethane (PU) with two biopolymers such as cellulose acetate (CA) and zein. Here, PU was used as the foundation polymer, was blended with CA and zein to achieve desirable properties such as better hydrophilicity, excellent cell attachment, proliferation and blood clotting ability. To prevent common clinical infections, an antimicrobial agent, streptomycin sulfate was incorporated into the electrospun fibers and its antimicrobial ability against the gram negative and gram positive bacteria were examined. The interaction between fibroblasts and the PU-CA and PU-CA-zein-drug scaffolds such as viability, proliferation, and attachment were characterized. PU-CA-zein-drug composite nanoscaffold showed enhanced blood clotting ability in comparison with pristine PU nanofibers. The presence of CA and zein in the nanofiber membrane improved its hydrophilicity, bioactivity and created a moist environment for the wound, which can accelerate wound recovery. PMID:24507360

Unnithan, Afeesh Rajan; Gnanasekaran, Gopalsamy; Sathishkumar, Yesupatham; Lee, Yang Soo; Kim, Cheol Sang

2014-02-15

346

Wound-dressing materials with antibacterial activity from electrospun gelatin fiber mats containing silver nanoparticles  

Microsoft Academic Search

Ultrafine gelatin fiber mats with antibacterial activity against some common bacteria found on burn wounds were prepared from a gelatin solution (22%w\\/v in 70vol% acetic acid) containing 2.5wt% AgNO3. Silver nanoparticles (nAg), a potent antibacterial agent, first appeared in the AgNO3-containing gelatin solution after it had been aged for at least 12h, with the amount of nAg increasing with increasing

Pim-on Rujitanaroj; Nuttaporn Pimpha; Pitt Supaphol

2008-01-01

347

Preparation and characterization of novel ?-chitin\\/nanosilver composite scaffolds for wound dressing applications  

Microsoft Academic Search

We developed novel ?-chitin\\/nanosilver composite scaffolds for wound healing applications using ?-chitin hydrogel with silver nanoparticles. The prepared nanosilver particles and nanocomposite scaffolds were characterized using SEM, FT-IR, XRD and TGA studies. The antibacterial, blood-clotting, swelling, cell attachment and cytotoxicity studies of the prepared composite scaffolds were evaluated. The prepared ?-chitin\\/nanosilver composite scaffolds were bactericidal against Escherichia coli and Staphylococcus

P. T. Sudheesh Kumar; S. Abhilash; K. Manzoor; S. V. Nair; H. Tamura; R. Jayakumar

2010-01-01

348

Photosensitive and biomimetic core-shell nanofibrous scaffolds as wound dressing.  

PubMed

Tissue engineered skin grafts that mimic the native extracellular matrix of skin has gained huge popularity among clinicians since they increase the survival rate of the patients. Phototherapy shows promising results with respect to acute and chronic pain relief, treatment of inflammatory conditions and promotion of wound healing. Here, we encapsulated a photosensitive polymer poly (3-hexylthiophene) (P3HT) and epidermal growth factor in the core-shell-structured Gelatin/poly(L-lactic acid)-co-poly-(?-caprolactone) nanofibers [Gel/PLLCL/P3GF(cs)] by coaxial spinning and studied the potential application of the Gel/PLLCL/P3GF(cs) nanofibrous scaffold as a novel skin graft. The proliferation of fibroblasts was significantly improved on Gel/PLLCL/P3GF(cs) under light stimulation compared to fibroblasts on the same scaffold under dark condition. Studies on the in vitro wound healing ability of Gel/PLLCL/P3GF(cs) showed complete closure of wound after 9 days under "light stimulation" too. Furthermore, the potential of adipose-derived stem cells (ASCs) to differentiate to epidermal cells on Gel/PLLCL/P3GF(cs) was evaluated. The differentiated ASCs with keratinocytes morphology were only found on the light stimulated Gel/PLLCL/P3GF(cs). Our results suggest that the photosensitive core-shell Gel/PLLCL/P3GF(cs) nanofibers could be a novel substrate to aid in the reestablishment of skin architecture. PMID:24417712

Jin, Guorui; Prabhakaran, Molamma P; Ramakrishna, Seeram

2014-05-01

349

An animal study of a novel tri-layer wound dressing material — non-woven fabric grafted with N-isopropyl acrylamide and gelatin  

Microsoft Academic Search

A novel method of preparation of easily stripped-off temporary wound dressing material is described. A tri-layer membrane system for artificial skin is prepared in this study. In this process, the N-isopropyl acrylamide monomer is successfully grafted on the non-woven fabric by co-polymerization. It is initiated by plasma to activate the surface of the non-woven cloth. N-isopropyl acrylamide is then grafted

Feng-Huei Lin; Tim-Mo Chen; Ko-Shao Chen; Te-Hsing Wu; Chia-Chieh Chen

2000-01-01

350

Radiation synthesis of poly(N-vinyl-2-pyrrolidone)-?-carrageenan hydrogels and their use in wound dressing applications. I. Preliminary laboratory tests  

Microsoft Academic Search

Poly(N-vinyl-2-pyrrolidone)--carrageenan hy- drogels (PVP-KC) were prepared by irradiating the mixtures of aqueous solutions of PVP, KC, potassium chloride, and poly(ethylene glycol) by -rays at different doses. Their preliminary laboratory tests were evaluated to identify their usability in wound dressing applications. For investigation of the effect of components on the gelation of PVP, sol- gel analyses were made and gel fractions

Murat ?en; Esra Nazan Avc?

2005-01-01

351

Diabetic Wound Care  

MedlinePLUS

... skin and tissue, called “debridement” Applying medication or dressings to the ulcer Managing blood glucose and other ... bandaged; cleanse the wound daily, using a wound dressing or bandage; and avoid walking barefoot. For optimum ...

352

Mechanical and microstructural properties of hybrid poly(ethylene glycol)-soy protein hydrogels for wound dressing applications.  

PubMed

Biomimetic hydrogel made of poly(ethylene glycol) and soy protein with a water content of 96% has been developed for moist wound dressing applications. In this study, such hybrid hydrogels were investigated by both tensile and unconfined compression measurements in order to understand the relationships between structural parameters of the network, its mechanical properties and protein absorption in vitro. Elastic moduli were found to vary from 1 to 17 kPa depending on the composition, while the Poisson's ratio (approximately 0.18) and deformation at break (approximately 300%) showed no dependence on this parameter. Further calculations yielded the crosslinking concentration, the average molecular weight between crosslinks (M(C)) and the mesh size. The results show that reactions between PEG and protein create polymeric chains comprising molecules of PEG and protein fragments between crosslinks. M(C) is three times higher than that expected for a "theoretical network." On the basis of this data, we propose a model for the 3D network of the hydrogel, which is found to be useful for understanding drug release properties and biomedical potential of the studied material. PMID:17380500

Snyders, Rony; Shingel, Kirill I; Zabeida, Oleg; Roberge, Christophe; Faure, Marie-Pierre; Martinu, Ludvik; Klemberg-Sapieha, Jolanta E

2007-10-01

353

A direct-electrospinning process by combined electric field and air-blowing system for nanofibrous wound-dressings  

NASA Astrophysics Data System (ADS)

An electrospinning process has been introduced to fabricate micro/nanofiber membranes having high porosity and specific surface area. When constantly/uniformly depositing the micro/nanofiber membrane on a target, the electrospun fibers require flushing out of the high charge and excessive remaining solvent built up, since these factors can interrupt the constant deposition rate of the electrospun fibers on substrates. These limitations can be overcome with a direct-electrospinning process, which can lower the charges of the electrospun fibers through a window of guiding electrodes and remaining solvent of the electrospun fibers during the spinning process by an air-blowing system. Because of the reduced charge accumulation of the electrospun fibers, the micro/nanofibers can be deposited on any kind of target, which may be a conductive or a non-conductive material. The fabricated membrane had a dramatically reduced charge, remaining solvent concentration, sufficient tensile modulus, and small pore-size distribution. To observe the possibility as a biomedical wound-dressing material, a bacteria-shielding test of the fabricated membrane was conducted.

Kim, Geun Hyung; Yoon, Hyeon

2008-03-01

354

Sodium alginate/poly(vinyl alcohol)/nano ZnO composite nanofibers for antibacterial wound dressings.  

PubMed

Sodium alginate (SA)/poly (vinyl alcohol) (PVA) fibrous mats were prepared by electrospinning technique. ZnO nanoparticles of size ?160nm was synthesized and characterized by UV spectroscopy, dynamic light scattering (DLS), XRD and infrared spectroscopy (IR). SA/PVA electrospinning was further carried out with ZnO with different concentrations (0.5, 1, 2 and 5%) to get SA/PVA/ZnO composite nanofibers. The prepared composite nanofibers were characterized using FT-IR, XRD, TGA and SEM studies. Cytotoxicity studies performed to examine the cytocompatibility of bare and composite SA/PVA fibers indicate that those with 0.5 and 1% ZnO concentrations are less toxic where as those with higher concentrations of ZnO is toxic in nature. Cell adhesion potential of this mats were further proved by studying with L929 cells for different time intervals. Antibacterial activity of SA/PVA/ZnO mats were examined with two different bacteria strains; Staphylococcus aureus and Escherichia coli, and found that SA/PVA/ZnO mats shows antibacterial activity due to the presence of ZnO. Our results suggest that this could be an ideal biomaterial for wound dressing applications once the optimal concentration of ZnO which will give least toxicity while providing maximum antibacterial activity is identified.f. PMID:21635916

Shalumon, K T; Anulekha, K H; Nair, Sreeja V; Nair, S V; Chennazhi, K P; Jayakumar, R

2011-10-01

355

The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models.  

PubMed

We determined whether a two-part space-conforming polyethylene glycol/dopa polymer-based gel promoted healing of contaminated wounds in mice. This silver-catalysed gel was previously developed to be broadly microbiocidal in vitro while being biocompatible with human wound cell functioning. Full-thickness wounds were created on the backs of mice. The wounds were inoculated with 10(4) CFU of each of four common skin wound contaminants, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumanii and Clostridium perfringens. The wounds were then treated with our multifunctional polymer-based gel, the commercially available NewSkin product, or left to heal untreated. The untreated wounds were overtly infected, and presented detectable bacterial loads over the entire 21-day healing period, while the gel and NewSkin groups presented significantly smaller rises in bacterial levels and were cleared of detectable colonies by the third week, with the gel group clearing the bacteria earlier. While all three groups healed their wounds, the polymer-based gel-treated group demonstrated significantly earlier re-epithelialization and dermal maturation (P<0.05). This was reflected in a quick regain of tensile strength. This accelerated dermal maturation and regain in strength was noted in mice treated with the polymer-based gel when compared to wound treated with the commercially available Aquacel-Ag dressing (P<0.05). What distinguishes the polymer-based gel from these other products is that it is incorporated within the healing wound. These preclinical studies show that the anti-microbial polymer gel not only supports but also accelerates healing of bacterially contaminated wounds. PMID:17561250

Yates, Cecelia C; Whaley, Diana; Babu, Ranjith; Zhang, Jianying; Krishna, Priya; Beckman, Eric; Pasculle, A William; Wells, Alan

2007-09-01

356

Collagen-grafted temperature-responsive nonwoven fabric for wound dressing  

NASA Astrophysics Data System (ADS)

Polypropylene (PP) nonwoven fabric (NWF) was modified by direct current pulsed plasma followed by grafting with acrylic acid (AAc) to improve its surface hydrophilicity and to introduce carboxylic acid group on the surface for further conjugation with bioactive collagen biomolecule. To endow temperature-responsive property, PP- g-collagen NWF was further modified with poly( N-isopropylacrylamide) (PNIPAAm). Experimental results demonstrated that the amount of AAc and collagen grafted were 43.4 nmole/cm 2, and 35.9 ?g/cm 2, respectively. The amount of PNIPAAm immobilized was 213 ?g/cm 2. The physical properties, surface chemical composition, and microstructure of the NWFs were characterized. From animal study, modified NWFs were found to promote wound healing with bigraft PP- g-collagen- g-PNIPAAm NWF showing the best performance.

Chen, Jyh-Ping; Lee, Wen-Li

2008-11-01

357

Systems-based approaches toward wound healing  

PubMed Central

Wound healing in the pediatric patient is of utmost clinical and social importance, since hypertrophic scarring can have aesthetic and psychological sequelae, from early childhood to late adolescence. Wound healing is a well-orchestrated reparative response affecting the damaged tissue at the cellular, tissue, organ, and system scales. While tremendous progress has been made towards understanding wound healing at the individual temporal and spatial scales, its effects across the scales remain severely understudied and poorly understood. Here we discuss the critical need for systems-based computational modeling of wound healing across the scales, from short-term to long-term and from small to large. We illustrate the state of the art in systems modeling by means of three key signaling mechanisms: oxygen tension regulating angiogenesis and revascularization; TGF-? kinetics controlling collagen deposition; and mechanical stretch stimulating cellular mitosis and extracellular matrix remodeling. The complex network of biochemical and biomechanical signaling mechanisms and the multi-scale character of the healing process make systems modeling an integral tool in exploring personalized strategies for wound repair. A better mechanistic understanding of wound healing in the pediatric patient could open new avenues in treating children with skin disorders such as birth defects, skin cancer, wounds, and burn injuries.

Buganza-Tepole, Adrian; Kuhl, Ellen

2013-01-01

358

Evidence-based recommendations for negative pressure wound therapy: treatment variables (pressure levels, wound filler and contact layer)--steps towards an international consensus.  

PubMed

Negative pressure wound therapy (NPWT) is becoming a commonplace treatment in many clinical settings. New devices and dressings are being introduced. Despite widespread adoption, there remains uncertainty regarding several aspects of NPWT use. To respond to these gaps, a global expert panel was convened to develop evidence-based recommendations describing the use of NPWT. In a previous communication, we have reviewed the evidence base for the use of NPWT within trauma and reconstructive surgery. In this communication, we present results of the assessment of evidence relating to the different NPWT treatment variables: different wound fillers (principally foam and gauze); when to use a wound contact layer; different pressure settings; and the impact of NPWT on bacterial bioburden. Evidence-based recommendations were obtained by a systematic review of the literature, grading of evidence and drafting of the recommendations by a global expert panel. Evidence and recommendations were graded according to the Scottish Intercollegiate Guidelines Network (SIGN) classification system. In general, there is relatively weak evidence on which to base recommendations for any one NPWT treatment variable over another. Overall, 14 recommendations were developed: five for the choice of wound filler and wound contact layer, four for choice of pressure setting and five for use of NPWT in infected wounds. With respect to bioburden, evidence suggests that reduction of bacteria in wounds is not a major mode of action of NPWT. PMID:21868296

Birke-Sorensen, H; Malmsjo, M; Rome, P; Hudson, D; Krug, E; Berg, L; Bruhin, A; Caravaggi, C; Chariker, M; Depoorter, M; Dowsett, C; Dunn, R; Duteille, F; Ferreira, F; Francos Martínez, J M; Grudzien, G; Ichioka, S; Ingemansson, R; Jeffery, S; Lee, C; Vig, S; Runkel, N; Martin, R; Smith, J

2011-09-01

359

Potentials of Chitosan-Based Delivery Systems in Wound Therapy: Bioadhesion Study  

PubMed Central

Chitosan is currently proposed to be one of the most promising polymers in wound dressing development. Our research focuses on its potential as a vehicle for nano-delivery systems destined for burn therapy. One of the most important features of wound dressing is its bioadhesion to the wounded site. We compared the bioadhesive properties of chitosan with those of Carbopol, a synthetic origin polymer. Chitosan-based hydrogels of different molecular weights were first analyzed by texture analysis for gel cohesiveness, adhesiveness and hardness. In vitro release studies showed no difference in release of model antimicrobial drug from the different hydrogel formulations. Bioadhesion tests were performed on pig ear skin and the detachment force, necessary to remove the die from the skin, and the amount of remaining formulation on the skin were determined. Although no significant difference regarding detachment force could be seen between Carbopol-based and chitosan-based formulations, almost double the amount of chitosan formulation remained on the skin as compared to Carbopol formulations. The findings confirmed the great potential of chitosan-based delivery systems in advanced wound therapy. Moreover, results suggest that formulation retention on the ex vivo skin samples could provide deeper insight on formulation bioadhesiveness than the determination of detachment force.

Hurler, Julia; Skalko-Basnet, Natasa

2012-01-01

360

A prospective two-armed trial assessing the efficacy and performance of a silver dressing used postoperatively on high-risk, clean surgical wounds.  

PubMed

Surgical site infections (SSI) are a known complication of surgery. Silver-containing wound treatments are popular, despite the lack of evidence of SSI reduction. A two-armed study was conducted between July 2007 and November 2008 to evaluate the efficacy and ease of use of a postoperative silver dressing. In the first arm of the study, patients undergoing clean general, vascular, orthopedic, and neurosurgical procedures were allocated to receive a postoperative silver dressing (POSD) or a standard dressing of nonstick gauze under a fluid occlusive dressing. Outcome variables included the incidence of antibiotic initiation for SSI, clinical signs of infection, and leukocyte counts. The second arm of the study was a prospective case series designed to evaluate the performance and handling characteristics of the POSD. Onehundred- ninety-nine (199) patients (mean age 59.2 [range 21-94] years) were enrolled in the first arm of the study. Three out of 99 (3%) patients in the POSD and six out of 100 (6%) control group patients received antibiotic therapy for SSI (P = 0.498). Differences in the percentage of patients with clinical signs of infection following surgery also were not statistically significant (POSD: n = 24, 24.2%; control: n = 30, 30%; P = 0.426). In the second arm, 34 out of 36 patients rated the study dressing easy to apply in (94%), and no pain on removal was noted in 38 out of 57 (66.7%) assessments. No patients in the dressing performance cohort developed an SSI. Prospective, randomized, controlled clinical studies with large sample sizes are warranted to evaluate the efficacy and cost-effectiveness of the POSD. PMID:24706401

Schwartz, Jamie; Goss, Selena; Facchin, Federico; Manizate, Fotini; Gendics, Cynthia; Braitman, Elissa; Lantis, John

2014-04-01

361

Cost Control Technology of Ore-dressing Production Process Based on Neural Network and its Application  

Microsoft Academic Search

According to the current status of production cost management of ore-dressing production in China, this paper proposes a technology diagram of cost control of production process to realize the optimum control of material consumption of ore-dressing and builds up a cost predicting model and analysis model based on neural networks. The two models above have been applied in the cost

Xin Yang; Tianyong Chai; Wei Liu

2005-01-01

362

Gauze Packing of Open Surgical Wounds: Empirical or Evidence-Based Practice?  

PubMed Central

INTRODUCTION Most surgical wounds are closed primarily, but some are allowed to heal by secondary intention. This usually involves repeated packing and dressing of the raw wound surfaces. Although the long-term care of such wounds has devolved to the care of nurses in the community or out-patient setting, the initial wound dressing or cavity packing is done by the surgeon in the operating theatre. Many surgeons are unaware of the growth of the discipline of wound care, and still use traditional soaked gauze for dressing and packing open surgical wounds and cavities. RESULTS This review summarises the some of the modern alternatives available and the evidence – or the lack of it – for their use in both the acute and chronic setting.

Dinah, F; Adhikari, A

2006-01-01

363

A comparison of a modified form of Granuflex (Granuflex Extra Thin) and a conventional dressing in the management of lacerations, abrasions and minor operation wounds in an accident and emergency department.  

PubMed Central

A clinical study of 96 patients compared a new hydrocolloid dressing (Granuflex Extra Thin) with a non-adherent dressing (perforated film absorbent dressing) in the management of lacerations, abrasions and minor operation incisions at the Accident and Emergency (A&E) Department of the University College Hospital, Galway. While time to heal was similar for both groups, the patients using Granuflex Extra Thin experienced less pain (P < 0.001), required less analgesia (P = 0.0154) and were able to carry out their normal daily activities including bathing or showering without affecting the dressing or the wound. Patient satisfaction with the new dressing appeared to be very high especially in those patients who pursued an active lifestyle.

Heffernan, A; Martin, A J

1994-01-01

364

Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems.  

PubMed

Novel hybrid nanomaterials have been developed for antimicrobial applications. Here we introduce a green route to produce antibacterial nanofiber mats loaded with silver nanoparticles (Ag-NPs, 25 nm diameter) enveloped in chitosan after reduction with glucose. The nanofiber mats were obtained from colloidal dispersions of chitosan-based Ag-NPs blended with polyvinyl alcohol. Nanofibers (150 nm average diameter and narrow size distribution) were obtained by electrospinning and cross-linked with glutaraldhyde. The effect of crosslinking on the release of silver was studied by atomic absorption spectroscopy. Antimicrobial activity was studied by the viable cell-counting; mats loaded with silver and control samples (chitosan/PVA) with different degrees of cross-linking were compared for their effectiveness in reducing or halting the growth of aerobic bacteria. The results showed superior properties and synergistic antibacterial effects by combining chitosan with Ag-NPs. PMID:24188851

Abdelgawad, Abdelrahman M; Hudson, Samuel M; Rojas, Orlando J

2014-01-16

365

[Bioactive dressings].  

PubMed

Wound healing is a complex process involving several cell types (keratinocytes, fibroblasts, endothelial cells, etc.) as well as many growth factors (PDGF, TGF-betas, FGFs, VEGF, etc.). It can be challenging when wounds are deep or very large (third degree burn, ulceration after cutaneous tumor resection) or in presence of peripheral vascular disease, metabolic disturbances or peripheral neuropathy (chronic vascular or diabetic wounds). In order to promote skin regeneration, numerous bioactive dressings combining cells, matrices and growth factors are available on the market. This article provides a general overview of the various product categories and presents their main indications. The principal axes of the biomedical research in this area are also discussed. PMID:20229727

Grasset, N; Raffoul, W; Bigliardi, P

2010-02-17

366

Novel cellulose based materials for safe and efficient wound treatment.  

PubMed

The present study aims at achieving effects of improved hydrophilicity and microorganism inhibition, which are rarely simultaneously present in wound dressings. Viscose fibers in their non-woven form were modified using two different pathways. Effects of a two-step procedure, i.e. alkaline or oxygen plasma treatment followed by the attachment of silver chloride nanoparticles were compared to a one-step procedure, i.e. ammonium plasma treatment, which results in both desired material characteristics simultaneously. The surface properties of untreated and differently modified cellulose samples were analyzed by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), in vitro silver release, and hydrophilicity measurements. The treatment effect on antimicrobial activity was determined by the AATCC 100-1999 standard test. In light of the introduced wound dressing preparation procedures and the desired wound dressing characteristics, the effectiveness of the used procedures was evaluated. Antimicrobial activity was proven against all Gram negative bacteria, while the Gram positive bacteria survive the as-prepared samples. Hydrophilicity was proven to be excellent using both preparation procedures. The mentioned results prove the potential of the used procedures and encourage future developments toward the clinical proof of concept. PMID:24188838

Peršin, Zdenka; Maver, Uroš; Pivec, Tanja; Maver, Tina; Vesel, Alenka; Mozeti?, Miran; Stana-Kleinschek, Karin

2014-01-16

367

The effect of moist and moist exposed dressings on healing and barrier function restoration of partial thickness wounds  

Microsoft Academic Search

Improved healing of full- and partial-thickness cutaneous wounds in wet and moist environments is due primarily to retention of biological fluids over the wound preventing desiccation of denuded dermis or deeper tissues. This also allows faster and unimpeded migration of keratinocytes over the wound surface and enables the naturally occurring cytokines and growth factors to exert their beneficial effect on

B. S. Atiyeh; C. A. Al-Amm; K. A. El-Musa; A. Sawwaf; R. Dham

2003-01-01

368

Cyanoacrylate Glue Dressing for Hypospadias Surgery  

PubMed Central

Background: Hypospadias repair is a common pediatric operation. Several kinds of dressings are currently available, with their benefits and side effects. Aim: The aim of our study was to introduce a new method of dressing, by pouring several layers of cyanoacrylate (CA) glue as the dressing, in hypospadias surgery. Materials and Methods: Twenty out of the 61 patients with hypospadias, with a mean age of 13.5 months, were enrolled in this study. Forty-one had conventional dressing (pressure wrap dressing), while CA glue was used in 20 patients. CA glue was applied around the penis and its base four times and each time it took one minute to dry. All patients were followed postoperatively for two weeks and six to twelve months, for early and late complications, respectively. Results: One out of 20 developed wound hematoma, one had skin necrosis with infection, and edema was present in all. All these were easily diagnosed and managed rapidly. In the group treated with conventional methods, there were five infections, seven hematomas, all the patients in the group had edema and painful removal of the dressing, and 10 needed repeat dressing. Conclusions: CA glue is impermeable to urine and stool, and prevents edema and hematoma. Used in several layers, it is a good alternative dressing in hypospadias surgery.

Hosseini, Seyed Mohammad Vahid; Rasekhi, Ahmad Reza; Zarenezhad, Mohammad; Hedjazi, Arya

2012-01-01

369

[Nanocomposite regeneration systems for wound healing].  

PubMed

The regeneration system "Biokol" based on the principles of combining the synthetic and natural biopolymers has been investigated. The wound dressing consists of "large" (200-250 nm) particles of a synthetic biopolymer and a gel component, which constitutes "small" polysaccharide elements 10-20 nm in size. The system can be used both separately and in combination with the gel complex and cell cultures. In properties (vapor and gas permeability, mechanical properties, prokhodimost conductivity, resistance to microbes, etc.), it corresponds to the upper layer of the skin. When applied to the wound, the dressing changes its adhesiveness: first, owing to its hydrophilicity and low hydrophobicity, it closely adheres to the wound surface, and after some time, which corresponds to the time the polysaccharide complex is released from the dressing, it becomes hydrophobic and easily separates from the wound. Owing to these properties, the system can be used at all stages of the wound healing. PMID:22279760

Gavriliuk, B K; Gavriliuk, V B

2011-01-01

370

The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse models  

PubMed Central

We determined whether a two part space-conforming polyethyleneglycol/dopa polymer-based gel promoted healing of contaminated wounds in mice. This silver-catalysed gel was previously developed to be broadly microbiocidal in vitro while being biocompatible with human wound cell functioning. Full-thickness wounds were created on the backs of mice. The wounds were inoculated with 104 CFU of each of four common skin wound contaminants, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumanii and Clostridium perfringens. The wounds were then treated with our multifunctional polymer-based gel, the commercially-available NewSkin product, or left to heal untreated. The untreated wounds were overtly infected, and presented detectable bacterial loads over the entire 21 day healing period, while the gel and NewSkin groups presented significantly smaller rises in bacterial levels and were cleared of detectable colonies by the third week, with the gel group clearing the bacteria earlier. While all three groups healed their wounds, the polymer-based gel treated group demonstrated signficantly earlier re-epithelialization and dermal maturation (P < 0.05). This was reflected in a quick regain of tensile strength. This accelerated dermal maturation and regain in strength was noted in mice treated with the polymer-based gel when compared to wound treated with the commercially-available Aquacel-Ag dressing (P < 0.05). What distinguishes the polymer-based gel from these other products is that is incorporated within the healing wound. These preclinical studies show that the anti-microbial polymer gel not only supports but also accelerates healing of bacterially contaminated wounds.

Yates, Cecelia Christina; Whaley, Diana; Babu, Ranjith; Zhang, Jianying; Krishna, Priya; Beckman, Eric; Pasculle, A. William; Wells, Alan

2007-01-01

371

Iodine released from the wound dressing iodosorb modulates the secretion of cytokines by human macrophages responding to bacterial lipopolysaccharide  

Microsoft Academic Search

Clinical data suggests that iodine released into the wound environment by Iodosorb may enhance the healing of chronic leg ulcers by a mechanism additional to its anti-bacterial activity. The macrophage is considered to play a central role in controlling wound healing and this study was designed to determine whether interaction with iodine could modulate macrophage cytokine output. The human macrophage

K. Moore; A. Thomas; K. G. Harding

1997-01-01

372

A simple postoperative umbilical negative-pressure dressing.  

PubMed

Application of umbilical wound dressings is challenging because of the shape and depth of the navel. Dressing changes and umbilical wound cleaning as practiced by many surgeons are painful procedures. The authors describe a simple and durable postoperative umbilical vacuum dressing, providing the advantages of topical negative-pressure wound therapy. PMID:23263397

Seifarth, Federico G; Knight, Colin G

2013-01-01

373

Radiation synthesis of poly(N-vinyl-2-pyrrolidone)-kappa-carrageenan hydrogels and their use in wound dressing applications. I. Preliminary laboratory tests.  

PubMed

Poly(N-vinyl-2-pyrrolidone)-kappa-carrageenan hydrogels (PVP-KC) were prepared by irradiating the mixtures of aqueous solutions of PVP, KC, potassium chloride, and poly(ethylene glycol) by gamma-rays at different doses. Their preliminary laboratory tests were evaluated to identify their usability in wound dressing applications. For investigation of the effect of components on the gelation of PVP, sol-gel analyses were made and gel fractions of the hydrogels were determined. Mechanical experiments were conducted for both unirradiated and irradiated samples. For investigation of the fluid uptake capacity of the hydrogels, swelling experiments were performed in pseudo-extracellular fluid solution at various temperatures. Acidity/alkalinity (pH) and electrical conductivity tests were achieved from aqueous extracts of hydrogels, and bioadhesion strength of the hydrogels was investigated on human skin. PMID:15962270

Sen, Murat; Avci, Esra Nazan

2005-08-01

374

A novel technique for the treatment of infected metalwork in orthopaedic patients using skin closure over irrigated negative pressure wound therapy dressings  

PubMed Central

Introduction There has been recent interest in the use of negative pressure wound therapy (NWPT) as an adjunct to parenteral antibiotics in the treatment of infection in orthopaedic patients with metalwork in situ. To address some of the limitations of standard NPWT in this situation, the senior author has developed a modified method of treatment for infected metalwork (excluding arthroplasty) in orthopaedic patients that includes irrigation and skin closure over the standard NPWT dressing. Methods This retrospective study examined the outcome of a case series of 16 trauma and orthopaedic patients with deep infection involving metalwork in whom this modified form of NPWT was used. In conjunction with standard parenteral antibiotic therapy and a multidisciplinary approach, this modified technique included serial debridements in theatre, irrigation and negative pressure dressings over a white polyvinyl alcohol foam (KCI, Kidlington, UK) as well as closure of the skin over the foam. Results Among the 16 patients, there was a variety of upper and lower limb as well as spinal trauma and elective cases. In all 16 patients, there was successful resolution of the infection with no early or unplanned removal of any metalwork required. Conclusions Patients with infected metalwork are a heterogeneous group, and often suffer high morbidity and mortality. The modified NPWT technique shows potential as an adjunct in the treatment of complex orthopaedic patients with infected metalwork.

Chapman, AWP; Krikler, S; Krkovic, M

2013-01-01

375

Asymmetric chitosan membranes prepared by dry\\/wet phase separation: a new type of wound dressing for controlled antibacterial release  

Microsoft Academic Search

An AgSD-incorporated chitosan membrane with sustained antimicrobial capability has been developed by a dry\\/wet phase separation method to overcome current limitations in silver sulfadiazine (AgSD) cream for treating acute burn wounds. The asymmetric chitosan membrane consists of a dense skin and sponge-like porous layer, which can fit the requirements (oxygen permeability, controlled water vapor evaporation and the drainage of wound

Fwu-Long Mi; Yu-Bey Wu; Shin-Shing Shyu; An-Chong Chao; Juin-Yih Lai; Chia-Ching Su

2003-01-01

376

A povidone-iodine medicated dressing.  

PubMed

The iodine content of a tulle gras-type dressing medicated with povidone-iodine (Poviderm) has been measured and its potential efficacy in wound care explored by means of laboratory models. Simple tests demonstrated the ready diffusibility and antibacterial activity of povidone-iodine. Wound models clearly showed that the limiting factor for useful dressing life is extent of exudation. It seems likely that this dressing would provide good topical antibacterial prophylaxis and may reduce the bacterial burden of colonised wounds. The dressing should help contain wound bacteria and thus assist infection control. PMID:9791357

Lawrence, J C

1998-07-01

377

Collagen-based bioadhesives for wound closure  

NASA Astrophysics Data System (ADS)

In this paper, we reported the results of an in vitro pilot study designed to evaluate the possible use of type-I collagen for corneal wound closure via an in situ photo-crosslink process. The wound strength was measured by rising the intraocular pressure (IOP) at a constant rate until wound breakage (blow-up test). In addition, histology was performed to study the wound appearance after photosoldering.

Ren, Qiushi; Simon, Gabriel; Parel, Jean-Marie A.

1992-08-01

378

Managing chronic, nonhealing wounds using a research-based protocol.  

PubMed

The physical, emotional, and financial costs of caring for patients with chronic, nonhealing leg wounds are substantial. In fiscal year 2001, the home care department of a large Veterans Affairs medical center in the Pacific Northwest spent nearly half of its annual budget on veterans needing wound care. In this article, the authors describe a practice improvement project designed to improve the wound care management of homebound veterans with chronic, nonhealing lower extremity wounds using a research-based protocol and consultation by a certified wound care specialist, as well as the effect of this program on home care expenditures. PMID:18019117

Benton, Nancy; Harvath, Theresa A; Flaherty-Robb, Marna; Medcraft, Marijo; McWhorter, Karen; McClelland, Faith; Joseph, Carol; Mambourg, Floris

2007-11-01

379

Use of electron beam for the production of hydrogel dressings  

NASA Astrophysics Data System (ADS)

The electron beam irradiation technique has been utilized to prepare hydrogel wound dressings. The composition of the dressings is based on polyvinylpyrrolidone (PVP), poly(ethylene glycol) (PEG), and agar. Increasing the irradiation dose leads to an increase in the gel fraction; this increase is due to increased crosslink density. The gel fraction% decreases as the PEG concentration increases. The maximum swelling% decreases with increasing the irradiation dose, but increases with increasing the PEG concentration. This relationship can be utilized to modify the gel properties as gel fraction% and maximum swelling of the hydrogel. The prepared dressings could be considered as a good barrier against microbes.

Ajji, Z.; Mirjalili, G.; Alkhatab, A.; Dada, H.

2008-02-01

380

Thrombin production and human neutrophil elastase sequestration by modified cellulosic dressings and their electrokinetic analysis.  

PubMed

Wound healing is a complex series of biochemical and cellular events. Optimally, functional material design addresses the overlapping acute and inflammatory stages of wound healing based on molecular, cellular, and bio-compatibility issues. In this paper the issues addressed are uncontrolled hemostasis and inflammation which can interfere with the orderly flow of wound healing. In this regard, we review the serine proteases thrombin and elastase relative to dressing functionality that improves wound healing and examine the effects of charge in cotton/cellulosic dressing design on thrombin production and elastase sequestration (uptake by the wound dressing). Thrombin is central to the initiation and propagation of coagulation, and elastase is released from neutrophils that can function detrimentally in a stalled inflammatory phase characteristic of chronic wounds. Electrokinetic fiber surface properties of the biomaterials of this study were determined to correlate material charge and polarity with function relative to thrombin production and elastase sequestration. Human neutrophil elastase sequestration was assessed with an assay representative of chronic wound concentration with cotton gauze cross-linked with three types of polycarboxylic acids and one phosphorylation finish; thrombin production, which was assessed in a plasma-based assay via a fluorogenic peptide substrate, was determined for cotton, cotton-grafted chitosan, chitosan, rayon/polyester, and two kaolin-treated materials including a commercial hemorrhage control dressing (QuickClot Combat Gauze). A correlation in thrombin production to zeta potential was found. Two polycarboxylic acid cross linked and a phosphorylated cotton dressing gave high elastase sequestration. PMID:24956451

Edwards, Judson Vincent; Prevost, Nicolette

2011-01-01

381

Thrombin Production and Human Neutrophil Elastase Sequestration by Modified Cellulosic Dressings and Their Electrokinetic Analysis  

PubMed Central

Wound healing is a complex series of biochemical and cellular events. Optimally, functional material design addresses the overlapping acute and inflammatory stages of wound healing based on molecular, cellular, and bio-compatibility issues. In this paper the issues addressed are uncontrolled hemostasis and inflammation which can interfere with the orderly flow of wound healing. In this regard, we review the serine proteases thrombin and elastase relative to dressing functionality that improves wound healing and examine the effects of charge in cotton/cellulosic dressing design on thrombin production and elastase sequestration (uptake by the wound dressing). Thrombin is central to the initiation and propagation of coagulation, and elastase is released from neutrophils that can function detrimentally in a stalled inflammatory phase characteristic of chronic wounds. Electrokinetic fiber surface properties of the biomaterials of this study were determined to correlate material charge and polarity with function relative to thrombin production and elastase sequestration. Human neutrophil elastase sequestration was assessed with an assay representative of chronic wound concentration with cotton gauze cross-linked with three types of polycarboxylic acids and one phosphorylation finish; thrombin production, which was assessed in a plasma-based assay via a fluorogenic peptide substrate, was determined for cotton, cotton-grafted chitosan, chitosan, rayon/polyester, and two kaolin-treated materials including a commercial hemorrhage control dressing (QuickClot Combat Gauze). A correlation in thrombin production to zeta potential was found. Two polycarboxylic acid cross linked and a phosphorylated cotton dressing gave high elastase sequestration.

Edwards, Judson Vincent; Prevost, Nicolette

2011-01-01

382

Cytotoxicity and wound healing properties of PVA/ws-chitosan/glycerol hydrogels made by irradiation followed by freeze-thawing  

NASA Astrophysics Data System (ADS)

Hydrogels based on poly(vinyl alcohol), water-soluble chitosan and glycerol made by irradiation followed by freeze-thawing were evaluated as wound dressing. MTT assay suggested that the extract of hydrogels was nontoxic towards L929 mouse fibroblasts. Compared to gauze dressing, the hydrogel can accelerate the healing process of full-thickness wounds in a rat model. Wounds treated with hydrogel healed at 11th day postoperatively and histological observation showed that mature epidermal architecture was formed. These indicate that it is a good wound dressing.

Yang, Xiaomin; Yang, Kang; Wu, Shengwei; Chen, Xiliang; Yu, Feng; Li, Jungang; Ma, Mingwang; Zhu, Zhiyong

2010-05-01

383

Review collagen-based biomaterials for wound healing.  

PubMed

With its wide distribution in soft and hard connective tissues, collagen is the most abundant of animal proteins. In vitro, natural collagen can be formed into highly organized, three-dimensional scaffolds that are intrinsically biocompatible, biodegradable, nontoxic upon exogenous application, and endowed with high tensile strength. These attributes make collagen the material of choice for wound healing and tissue engineering applications. In this article, we review the structure and molecular interactions of collagen in vivo; the recent use of natural collagen in sponges, injectables, films and membranes, dressings, and skin grafts; and the on-going development of synthetic collagen mimetic peptides as pylons to anchor cytoactive agents in wound beds. © 2014 Wiley Periodicals, Inc. Biopolymers 101: 821-833, 2014. PMID:24633807

Chattopadhyay, Sayani; Raines, Ronald T

2014-08-01

384

Promotion of full-thickness wound healing using epigallocatechin-3-o-gallate/poly (lactic-co-glycolic Acid) membrane as temporary wound dressing.  

PubMed

Epigallocatechin-3-O-gallate (EGCG) is a major polyphenolic compound in green tea. It has been known that EGCG regulates the secretion of cytokines and the activation of skin cells during wound healing. In this study, various concentrations of EGCG were added to the electrospun membranes composed of poly (lactic-co-glycolic acid) (PLGA), and its healing effects on full-thickness wounds created in nude mice were investigated. The electrospun membranes containing 5 wt% EGCG (5EGCG/PLGA membrane) exhibited cytotoxicity in human dermal fibroblasts (HDFs) as HDF morphologies were transformed on them. In the animal study, cell infiltration of mice treated with electrospun membranes containing 1 wt% EGCG (1EGCG/PLGA membrane) significantly increased after 2 weeks. The immunoreactivity of Ki-67 (re-epithelialization at the wound site) and CD 31 (formation of blood vessels) also increased in the mice treated with 1EGCG/PLGA membranes in comparison with the mice treated with PLGA membranes. These results suggest that 1EGCG/PLGA can enhance wound healing in full thickness by accelerating cell infiltration, re-epithelialization, and angiogenesis. PMID:24571533

Kim, Hye-Lee; Lee, Jeong-Hyun; Kwon, Byeong Ju; Lee, Mi Hee; Han, Dong-Wook; Hyon, Suong-Hyu; Park, Jong-Chul

2014-05-01

385

Severe wound traction-blisters after inadequate dressing application following laparoscopic cholecystectomy: case report of a preventable complication  

Microsoft Academic Search

Background  The inadequate application of postoperative dressings can lead to significant complications, including skin injuries, compartment\\u000a syndromes, and potential limb loss. To our knowledge, the occurrence of post laparoscopic cholecystectomy related skin complications\\u000a have not yet been reported in the peer-reviewed literature.\\u000a \\u000a \\u000a \\u000a \\u000a Case Presentation  Following laparoscopic cholecystectomy for symptomatic gallstone disease, a seventy eight year old healthy white male broke\\u000a out in

Abayomi L Sanusi

2011-01-01

386

Cytotoxicity and wound healing properties of PVA\\/ws-chitosan\\/glycerol hydrogels made by irradiation followed by freeze–thawing  

Microsoft Academic Search

Hydrogels based on poly(vinyl alcohol), water-soluble chitosan and glycerol made by irradiation followed by freeze–thawing were evaluated as wound dressing. MTT assay suggested that the extract of hydrogels was nontoxic towards L929 mouse fibroblasts. Compared to gauze dressing, the hydrogel can accelerate the healing process of full-thickness wounds in a rat model. Wounds treated with hydrogel healed at 11th day

Xiaomin Yang; Kang Yang; Shengwei Wu; Xiliang Chen; Feng Yu; Jungang Li; Mingwang Ma; Zhiyong Zhu

2010-01-01

387

The clinical and cost effectiveness of bee honey dressing in the treatment of diabetic foot ulcers  

Microsoft Academic Search

Honey is known, since antiquity, as an effective wound dressing. Emergence of resistant strains and the financial burden of modern dressings, have revived honey as cost-effective dressing particularly in developing countries. Its suitability for all stages of wound healing suggests its clinical effectiveness in diabetic foot wound infections.Thirty infected diabetic foot wounds were randomly selected from patients presenting to Surgery

A. M. Moghazy; M. E. Shams; O. A. Adly; A. H. Abbas; M. A. El-Badawy; D. M. Elsakka; S. A. Hassan; W. S. Abdelmohsen; O. S. Ali; B. A. Mohamed

2010-01-01

388

AQUACEL(R) Ag Dressing with Hydrofiber(R) Technology  

PubMed Central

Problem There are many silver-containing wound dressings available for managing and preventing wound infection. Each claims to provide effective antimicrobial activity due to the presence of silver in the dressing. However, assuming effectiveness on the basis of an antimicrobial alone ignores the importance of the carrier dressing and its overall role in wound healing. Solution Choice of a silver-containing wound dressing should include consideration of the impact that dressing technology has on the effectiveness of antimicrobial activity as well as its influence on other factors essential for wound healing. New Technology AQUACEL® Ag dressing combines patented Hydrofiber® Technology with ionic silver, a proven broad-spectrum antimicrobial. Recent in vitro testing suggests that dressing technology may impact the antimicrobial effectiveness of silver-containing dressings as well as other factors relevant to wound healing. Specifically, a comparative in vitro study demonstrated how the ability of a dressing to micro-contour to the wound bed is critical to antimicrobial effectiveness. Compared with other silver dressings tested, AQUACEL Ag dressing minimized voids and spaces where bacteria can thrive, allowing the silver within the dressing to be in contact with the wound. AQUACEL Ag dressing was observed to kill more bacteria and control the spread of pathogens. Indications for Use AQUACEL Ag dressing is indicated for the management of a variety of at risk/infected chronic and acute wounds. Caution Not all silver dressings are created equal. Selection of an antimicrobial dressing should take into account in vitro data regarding antimicrobial efficacy of a dressing in addition to its ability to promote wound healing.

Hurlow, Jenny

2012-01-01

389

Silver Toxicity With the Use of Silver-Impregnated Dressing and Wound Vacuum-Assisted Closure in an Immunocompromised Patient  

Microsoft Academic Search

Silver-containing topical agents are used to help prevent infectious complications in wound therapy. Toxicity from topical silver agent exposure was initially reported in 1975 and was clinically characterized by granulocytopenia. Currently, the data regarding potential toxicity associated with silver-impregnated devices are limited. A 23-year-old patient receiving chemotherapy for acute lymphoblastic leukemia presented with necrotizing fasciitis of the abdominal wall and

Cabrini A. LaRiviere; Adam B. Goldin; Jeffrey Avansino

2011-01-01

390

Calcium-Based Nanoparticles Accelerate Skin Wound Healing  

PubMed Central

Introduction Nanoparticles (NPs) are small entities that consist of a hydroxyapatite core, which can bind ions, proteins, and other organic molecules from the surrounding environment. These small conglomerations can influence environmental calcium levels and have the potential to modulate calcium homeostasis in vivo. Nanoparticles have been associated with various calcium-mediated disease processes, such as atherosclerosis and kidney stone formation. We hypothesized that nanoparticles could have an effect on other calcium-regulated processes, such as wound healing. In the present study, we synthesized pH-sensitive calcium-based nanoparticles and investigated their ability to enhance cutaneous wound repair. Methods Different populations of nanoparticles were synthesized on collagen-coated plates under various growth conditions. Bilateral dorsal cutaneous wounds were made on 8-week-old female Balb/c mice. Nanoparticles were then either administered intravenously or applied topically to the wound bed. The rate of wound closure was quantified. Intravenously injected nanoparticles were tracked using a FLAG detection system. The effect of nanoparticles on fibroblast contraction and proliferation was assessed. Results A population of pH-sensitive calcium-based nanoparticles was identified. When intravenously administered, these nanoparticles acutely increased the rate of wound healing. Intravenously administered nanoparticles were localized to the wound site, as evidenced by FLAG staining. Nanoparticles increased fibroblast calcium uptake in vitro and caused contracture of a fibroblast populated collagen lattice in a dose-dependent manner. Nanoparticles also increased the rate of fibroblast proliferation. Conclusion Intravenously administered, calcium-based nanoparticles can acutely decrease open wound size via contracture. We hypothesize that their contraction effect is mediated by the release of ionized calcium into the wound bed, which occurs when the pH-sensitive nanoparticles disintegrate in the acidic wound microenvironment. This is the first study to demonstrate that calcium-based nanoparticles can have a therapeutic benefit, which has important implications for the treatment of wounds.

Ishise, Hisako; Carre, Antoine Lyonel; Nishimoto, Soh; Longaker, Michael; Lorenz, H. Peter

2011-01-01

391

A new smartphone-based method for wound area measurement.  

PubMed

Proper wound healing can be assessed by monitoring the wound surface area. Its reduction by 10 or 50% should be achieved after 1 or 4 weeks, respectively, from the start of the applied therapy. There are various methods of wound area measurement, which differ in terms of the cost of the devices and their accuracy. This article presents an originally developed method for wound area measurement. It is based on the automatic recognition of the wound contour with a software application running on a smartphone. The wound boundaries have to be traced manually on transparent foil placed over the wound. After taking a picture of the wound outline over a grid of 1?×?1?cm, the AreaMe software calculates the wound area, sends the data to a clinical database using an Internet connection, and creates a graph of the wound area change over time. The accuracy and precision of the new method was assessed and compared with the accuracy and precision of commercial devices: Visitrak and SilhouetteMobile. The comparison was performed using 108 wound shapes that were measured five times with each device, using an optical scanner as a reference device. The accuracy of the new method was evaluated by calculating relative errors and comparing them with relative errors for the Visitrak and the SilhouetteMobile devices. The precision of the new method was determined by calculating the coefficients of variation and comparing them with the coefficients of variation for the Visitrak and the SilhouetteMobile devices. A statistical analysis revealed that the new method was more accurate and more precise than the Visitrak device but less accurate and less precise than the SilhouetteMobile device. Thus, the AreaMe application is a superior alternative to the Visitrak device because it provides not only a more accurate measurement of the wound area but also stores the data for future use by the physician. PMID:24102380

Foltynski, Piotr; Ladyzynski, Piotr; Wojcicki, Jan M

2014-04-01

392

Tulle-gras dressings.  

PubMed

Tulle-gras backed with a soluble polyvinyl alcohol film has been compared with standard tulle-gras to treat patients with minor burns. The new material although less comformable to body contours offered the advantages of decreased dressing time and improved patient comfort. No adverse effects were observed on wound flora or healing rate. PMID:6509349

Hart, N B; Lawrence, J C

1984-10-01

393

NOTE: Measurement of skin dose variations produced by a silicon-based protective dressing in radiotherapy  

NASA Astrophysics Data System (ADS)

Variations in skin dose caused by a silicon-based burn dressing used in radiotherapy during treatment have been investigated. Measurement of these variations in skin dose has been achieved using thermoluminescent dosimeters (TLDs) and Gafchromic film. For a 6 MV x-ray beam results have shown that an approximately 0.4 mm thick silicon mesh dressing increases the average surface dose by approximately 12.5% to 14% of the maximum and average dose at 1 mm depth and by 4% to 6% of the maximum for field sizes ranging from 5 cm × 5 cm up to 40 cm × 40 cm at 100 cm source to surface distance (SSD). The radiation effective thickness of the silicon dressing was calculated to be 0.5 mm +/- 0.05 mm water equivalent. TLDs of various thicknesses provide point-dose assessment and Gafchromic film can provide a detailed two-dimensional dose map with a high spatial resolution. Results have shown that a large variation in skin dose is delivered under the dressing depending on the amount of material directly above it as defined by the silicon mesh outline.

Butson, Martin J.; Cheung, Tsang; Yu, Peter K. N.; Metcalfe, Peter

2002-06-01

394

Flexible sensors for chronic wound management.  

PubMed

Chronic nonhealing wounds are a major source of morbidity and mortality in bed-ridden and diabetic patients. Monitoring of physical and chemical parameters important in wound healing and remodeling process can be of immense benefit for optimum management of such lesions. Low-cost flexible polymeric and paper-based substrates are attractive platforms for fabrication of such sensors. In this review, we discuss recent advances in flexible physiochemical sensors for chronic wound monitoring. After a brief introduction to wound healing process and commercial wound dressings, we describe various flexible biocompatible substrates that can be used as the base platform for integration of wound monitoring sensors. We will then discuss several fabrication methods that can be utilized to integrate physical and chemical sensors onto such substrates. Finally, we will present physical and chemical sensors developed for monitoring wound microenvironment and outline future development venues. PMID:24802999

Ochoa, Manuel; Rahimi, Rahim; Ziaie, Babak

2014-01-01

395

Sea cucumber (Stichopus hermanii) based hydrogel to treat burn wounds in rats.  

PubMed

Malaysian sea cucumber was incorporated into hydrogel formulation by using electron beam irradiation technique and was introduced as novel cross-linked Gamat Hydrogel dressing. This study investigated whether Gamat Hydrogel enhanced repair of deep partial skin thickness burn wound in rats and its possible mechanism. Wounds were treated with either Gamat Hydrogel, control hydrogel, OpSite® film dressing or left untreated. Skin samples were taken at 7, 14, 21, and 28 days post burn for histological and molecular evaluations. Gamat Hydrogel markedly enhanced wound contraction and improved histological reorganization of the regenerating tissue. Furthermore, the dressing modulated the inflammatory responses, stimulated the activation and proliferation of fibroblasts, and enhanced rapid production of collagen fiber network with a consequently shorter healing time. The level of proinflammatory cytokines; IL-1?, IL-1?, and IL-6, were significantly reduced in Gamat Hydrogel treated wounds compared with other groups as assessed by reverse transcription-polymerase chain reaction (RT-PCR). In summary, our results showed that Gamat Hydrogel promoted burn wound repair via a complex mechanism involving stimulation of tissue regeneration and regulation of pro-inflammatory cytokines. The resultant wound healing effects were attributed to the synergistic effect of the hydrogel matrix and incorporated sea cucumber. PMID:21504052

Zohdi, Rozaini Mohd; Zakaria, Zuki Abu Bakar; Yusof, Norimah; Mustapha, Noordin Mohamed; Abdullah, Muhammad Nazrul Hakim

2011-07-01

396

Studies on gelatin-based sponges. Part III: A comparative study of cross-linked gelatin\\/alginate, gelatin\\/hyaluronate and chitosan\\/hyaluronate sponges and their application as a wound dressing in full-thickness skin defect of rat  

Microsoft Academic Search

Novel cross-linked sponges composed of gelatin\\/alginate and gelatin\\/hyaluronate and chitosan\\/hyaluronate (GH, GA and CH, respectively) were prepared and compared. Six different sponges with or without silver sulfadiazine (AgSD) were applied on the full-thickness dorsal skin defect of Wistar rat. The histology and epidermal wound healing rates of the skin defects were investigated by light microscopy and computerized morphometry 5 and

Y. S. Choi; S. B. Lee; S. R. Hong; Y. M. Lee; K. W. Song; M. H. Park

2001-01-01

397

Multifunctional Biomaterial Matrix for Advanced Wound Healing  

PubMed Central

Background Modern wound dressings provide a moist healing environment and facilitate faster and higher quality of healing. A new semi-interpenetrating network (sIPN) biomaterial platform based on poly(ethylene glycol) (PEG) and gelatin was developed as a multi-functional matrix for wound care. The Problem Besides providing a moist environment and facilitating the healing process, advanced wound dressings may be designed to serve as delivery matrices for drugs and therapeutic cells. New and effective treatments should also comply with clinical settings and be easy to use. No single treatment exists today that can fulfill all these requirements; however, advancement in multifunctional biomaterial design and development holds promise to fill this technology gap. Basic/Clinical Science Advances PEG?+?gelatin sIPN provides an ideal platform for fundamental research in cell-cell and cell-biomaterial interaction that is important in wound healing. The in situ forming ability of sIPN facilitates its use in large and irregular wounds with complex contours and crevices. Clinical Care Relevance Although various commercially available wound dressings have been produced, a low-cost, easy-to-use, and biofunctionalizable biomaterial that provides a moist environment and facilitates healing is still a target of active tissue regeneration research. Conclusion Extensive preclinical data support the use of in situ polymerized sIPN in advanced wound care.

Xu, Kedi; Kleinbeck, Kyle R.; Kao, Weiyuan John

2012-01-01

398

Development of new hydroactive dressings based on chitosan membranes: characterization and in vivo behavior.  

PubMed

Different poly(vinyl alcohol) (PVA)/chitosan lactate (ChL)-blended hydrogels containing nitrofurazone as a local anti-infective drug were prepared by the phase-inversion technique. The swelling degree, surface free energy, mechanical properties, and nitrofurazone release of these membranes were determined. Blood compatibility of these systems was evaluated by the open-static platelet adhesion test with whole human blood. The results showed that water absorption into the PVA/ChL membranes slowed down, governed by the rate at which the dressing interacted with the physiological fluid. Swelling degree values up to 200% were observed. The rate of release of nitrofurazone seemed to depend on the ChL percentage on the blend as well as the pH of the solution. The surface free energy values were in the range of 20-30 dynes/cm, which was appropriate for a favorable interaction with blood. From the Young's module curve, it could be seen that elastic hydrogels were obtained with increment of ChL in the PVA/ChL blends. Values of platelet adhesion and whole blood clotting times for the PVA/ChL blends as well as the increase of ChL, which appears to reduce the fibrinogen adsorption on the PVA/ChL membranes, demonstrated that the blood compatibility of PVP/ChL blends is superior to that separated polymers. The results of in vivo experiments in rats were in very good agreement with these observations, suggesting that PVA/ChL may serve as a new type of potential wound-dressing material. PMID:12483707

de Queiroz, Alvaro Antonio Alençar; Ferraz, Humberto Gomes; Abraham, Gustavo Abel; del Mar Fernández, María; Bravo, Antonio López; Román, Julio San

2003-01-01

399

Preparation of Sponge Using Porcine Small Intesinal Submucosa and Their Applications as a Scaffold and a Wound Dressing  

Microsoft Academic Search

Small intestine submucosa (SIS) derived from the submucosal layer of porcine intestine cause minimum immune response as acellular\\u000a collagen based matrix1,2 and moreover is a biodegradable.3 SIS consists of types I and III collagens above 90% and small amounts of types IV, V, and VI collagens.4 In addition, SIS contains a wide variety of cytokine such as basic fibroblast growth

Moon Suk Kim; Min Suk Lee; In Bum Song; Sang Jin Lee; Hai Bang Lee; Gilson Khang; Il Woo Lee

400

Antibacterial Ointments Versus Petrolatum-Based Ointments in Clean Wounds for Wound Healing  

Microsoft Academic Search

Background: Clean wounds are a frequent occurrence in the medical field and medical providers provide varied advice for wound care. Topical antibacterial ointments are commonly used to facilitate wound healing in patients with these types of wounds. Topical antibacterial ointments have been shown to cause allergic contact dermatitis, may cause an increase in antibiotic resistance, and may be equally efficacious

Kate Muirhead

2012-01-01

401

Postoperative wound care after dermatologic procedures: a comparison of 2 commonly used petrolatum-based ointments.  

PubMed

An appropriate selection of topical agents for wound care is important to promote uncomplicated healing. Petrolatum-based ointments, such as Aquaphor Healing Ointment (AHO) and white petroleum jelly, are commonly employed to keep wounds moist postoperatively. While they have beneficial properties for wound healing, they also may cause wound redness and swelling. We decided to evaluate for wound reactivity postoperatively for these 2 commonly used petrolatum-based ointments. We found that surgical wounds treated with AHO had a higher incidence of wound redness (52%) than those treated with plain white petrolatum (12%). PMID:23377388

Morales-Burgos, Adisbeth; Loosemore, Michael P; Goldberg, Leonard H

2013-02-01

402

Scanning electron microscopic examination of bacterial immobilisation in a carboxymethyl cellulose (AQUACEL ®) and alginate dressings  

Microsoft Academic Search

Dressings have been applied to open wounds for centuries. Traditionally they have been absorbent, permeable materials, i.e. gauze that could adhere to desiccated wound surfaces, inducing trauma on removal. With the advent of modern wound care products many dressings are now capable of absorbing large volumes of exudate whilst still continuing to provide a moist wound healing environment. Equally important

M Walker; J. A Hobot; G. R Newman; P. G Bowler

2003-01-01

403

Comparative evaluation of the antimicrobial activity of ACTICOAT antimicrobial barrier dressing.  

PubMed

This study evaluated the antimicrobial activity of ACTICOAT Antimicrobial Barrier Dressing (Westaim Biomedical Corp, Fort Saskatchewan, Alberta, Canada), a silver-coated wound dressing, and compared it with silver nitrate, silver sulfadiazine, and mafenide acetate. The minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC), zone of inhibition, and killing curves were determined with 5 clinically relevant bacteria. The data indicate that ACTICOAT silver had the lowest MIC and MBC and generated similar zones of inhibition to silver nitrate and silver sulfadiazine. Viable bacteria were undetectable 30 minutes after inoculation with the dressing, whereas it took 2 to 4 hours for silver nitrate and silver sulfadazine to achieve the same result. Mafenide acetate generated the biggest zones of inhibition, but it had the highest MICs and MBCs, and a significant number of bacteria still survived after 6 hours of treatment. The results suggest that ACTICOAT Antimicrobial Barrier Dressing has better antimicrobial performance than either of the existing silver-based products. ACTICOAT dressing killed the bacteria that were tested much faster, which is a very important characteristic for a wound dressing acting as a barrier to invasive infection to have. The study also suggests that a single susceptibility test such as a MIC or zone of inhibition test does not provide a comprehensive profile of antimicrobial activity of a topical antimicrobial agent or dressing. A combination of tests is desirable. PMID:10342470

Yin, H Q; Langford, R; Burrell, R E

1999-01-01

404

[The possibilities of using a chitin and chitosan in wounds treatment].  

PubMed

Chitin and chitosan are natural polysaccharides. In this study, we presented the possibilities of using chitin and chitosan in medical practice and experimental studies. Chitin and chitosan, based wound dressings available as commercial products, were presented. The directions of future progress in employment chitin and chitosan in treatment of many kinds of wounds were also described. In this study, the main properties of these polymers were established. The usefulness of the chitin and chitosan as hemostatic products, wound dressing and skin substitutes was emphasized. PMID:24596043

Mazurek, Piotr; Kuli?ski, Sebastian; Gosk, Jerzy

2013-01-01

405

Community Analysis of Chronic Wound Bacteria Using 16S rRNA Gene-Based Pyrosequencing: Impact of Diabetes and Antibiotics on Chronic Wound Microbiota  

Microsoft Academic Search

BackgroundBacterial colonization is hypothesized to play a pathogenic role in the non-healing state of chronic wounds. We characterized wound bacteria from a cohort of chronic wound patients using a 16S rRNA gene-based pyrosequencing approach and assessed the impact of diabetes and antibiotics on chronic wound microbiota.Methodology\\/Principal FindingsWe prospectively enrolled 24 patients at a referral wound center in Baltimore, MD; sampled

Lance B. Price; Cindy M. Liu; Johan H. Melendez; Yelena M. Frankel; David Engelthaler; Maliha Aziz; Jolene Bowers; Rogan Rattray; Jacques Ravel; Chris Kingsley; Paul S. Keim; Gerald S. Lazarus; Jonathan M. Zenilman; Adam J. Ratner

2009-01-01

406

Hemostatic dressings for the first responder: a review.  

PubMed

The military is interested in finding a hemostatic dressing that is effective in controlling hemorrhage from combat wounds, relatively inexpensive, and easy to transport. The fibrin dressing has existed for decades, but the military has been reluctant to use the dressing because it is not Food and Drug Administration approved, fairly expensive, and difficult to apply on certain wounds. Newer dressings such as the microporous polysaccharide hemosphere (TraumaDEX), mineral zeolite (QuikClot), poly-N-acetylglucosamine (HemCon), and microporous hydrogel-forming polyacrylamide (BioHemostat) dressings have addressed these deficiencies in that they are relatively inexpensive, easy to transport, and easy to apply. However, the effectiveness of these new dressings on wounds sustained in combat is still questionable according to studies and anecdotal reports from Operation Iraqi Freedom. More research is needed to draw definite conclusions about the effectiveness of these dressings in a combat setting. PMID:15495727

Neuffer, Marcus C; McDivitt, Jonathan; Rose, David; King, Kelly; Cloonan, Clifford C; Vayer, Joshua S

2004-09-01

407

Community-Based Care for Chronic Wound Management  

PubMed Central

Executive Summary In August 2008, the Medical Advisory Secretariat (MAS) presented a vignette to the Ontario Health Technology Advisory Committee (OHTAC) on a proposed targeted health care delivery model for chronic care. The proposed model was defined as multidisciplinary, ambulatory, community-based care that bridged the gap between primary and tertiary care, and was intended for individuals with a chronic disease who were at risk of a hospital admission or emergency department visit. The goals of this care model were thought to include: the prevention of emergency department visits, a reduction in hospital admissions and re-admissions, facilitation of earlier hospital discharge, a reduction or delay in long-term care admissions, and an improvement in mortality and other disease-specific patient outcomes. OHTAC approved the development of an evidence-based assessment to determine the effectiveness of specialized community based care for the management of heart failure, Type 2 diabetes and chronic wounds. Please visit the Medical Advisory Secretariat Web site at: www.health.gov.on.ca/ohtas to review the following reports associated with the Specialized Multidisciplinary Community-Based care series. Specialized multidisciplinary community-based care series: a summary of evidence-based analyses Community-based care for the specialized management of heart failure: an evidence-based analysis Community-based care for chronic wound management: an evidence-based analysis Please note that the evidence-based analysis of specialized community-based care for the management of diabetes titled: “Community-based care for the management of type 2 diabetes: an evidence-based analysis” has been published as part of the Diabetes Strategy Evidence Platform at this URL: http://www.health.gov.on.ca/english/providers/program/mas/tech/ohtas/tech_diabetes_20091020.html Please visit the Toronto Health Economics and Technology Assessment Collaborative Web site at: http://theta.utoronto.ca/papers/MAS_CHF_Clinics_Report.pdf to review the following economic project associated with this series: Community-based Care for the specialized management of heart failure: a cost-effectiveness and budget impact analysis. Objective The objective of this evidence-based review is to determine the effectiveness of a multidisciplinary wound care team for the management of chronic wounds