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1

The berberis story: Berberis vulgaris in therapeutics.  

PubMed

Barberry has played a prominent role in herbal healing for more than 2,500 years. Berberis vulgaris is a common garden bush, native to Europe and the British Isles, naturalized in North America, seems to have history as old as human race. Anthropologists believe in a ritual practice or sacred object, especially by Native Americans that it works as a supernatural power or as preventive or remedy of illness. It is a deciduous shrub having yellow flowers and scarlet colored fruit in the form of berries. Twenty two alkaloids have been reported so far from root, stem leaves and fruit of this plant, which are of medicinal importance. As a herbal remedy it has no match in serving human race since ancient times. It is the most widely used drug in Homeopathic system of medicine for kidney pain and for removal of kidney stones. In this article, we present countless blessings of nature encountered through this herb which are worthy of recording. PMID:17337435

Arayne, M Saeed; Sultana, Najma; Bahadur, Saima Sher

2007-01-01

2

Invasive Species Taxonomic Name: Berberis vulgaris  

E-print Network

Invasive Species Taxonomic Name: Berberis vulgaris Common Name: Common barberry Physical Description: Growth Type: Berberis vulgaris is a shrub that can grow up to 10 feet in height. Branches arch://ontariowildflowers.com/mondaygarden/article.php?id=0112 http://www.ruhr-uni-bochum.de/boga/html/Berberis_vulgaris_Foto.html http

Hayden, Nancy J.

3

Phytochemical Studies of Berberis vulgaris  

Microsoft Academic Search

This study presents a phytochemical investigation of the fruits of Berberis vulgaris Linn. The isolation and structure elucidation of four compounds are discussed. The terpenoids lupeol (1) and oleanolic acid (2) and the steroids stigmasterol (3) and stigmasterol glucoside (4) are isolated for the first time from this plant. The structure and stereochemistry at various asymmetric centers were established by

S. Saied; S. Begum

2004-01-01

4

Isoquinoline alkaloids from Berberis Vulgaris subsp. Australis  

Microsoft Academic Search

Sixteen isoquinoline alkaloids were isolated from Berberis vulgaris subsp. australis. In addition to quaternary protoberberines and bisbenzylisoquinolines, a new seco-bisbenzylisoquinoline, (-)-tejedine, is reported.

Rafael Suau; Rodrigo Rico; J. Manuel López-Romero; Francisco Nájera; Ana Cuevas

1998-01-01

5

First report of graft-transmission and PCR detection of Candidatus Liberibacter asiaticus from Atalantia buxifolia in Guangdong, China  

Technology Transfer Automated Retrieval System (TEKTRAN)

Atalantia buxifolia (Jiubingle), syn. Severinia buxifolia, is a common landscape plant and a popular Chinese medicinal herb known as Dongfengjie. It is unclear if this Rutaceous plant is a host for Candidatus Liberibacter asiaticus, the pathogen of citrus Huanglongbing (HLB) in Guangdong, P. R. Chin...

6

Antimicrobial activity of Scutia buxifolia against the honeybee pathogen Paenibacillus larvae.  

PubMed

The honeybee disease American foulbrood (AFB) is a serious problem since its causative agent (Paenibacillus larvae) has become increasingly resistant to conventional antibiotics. One of the feasible alternative treatments being used for control of this disease are plants extracts. The aim of the present work was to evaluate the effect of crude extract and fractions of Scutia buxifolia against six Paenibacillus species, including P. larvae, and its potential use for the control of AFB. In vitro activity of S. buxifolia samples against Paenibacillus species were evaluated by the disk diffusion and microdilution methods, and the minimal inhibitory concentration (MIC) were also determined. All Paenibacillus species were sensitive to crude extract and fractions of S. buxifolia. The dichloromethane (DC) fraction showed the better MIC (1.56 mg/mL), followed by ethyl acetate (EtAc) (6.25 mg/mL), n-butanol (BuOH) (25 mg/mL) and Crude extract (CE) (50 mg/mL). Toxic effect of S. buxifolia crude extracts and fractions against bees were also evaluated by the spraying application method of the same concentrations of MICs. The samples tested showed no toxic effects for the bees after 15 days of observation. These results are first time described for this species and showed that S. buxifolia presented a important activity against Paenibacillus species and proved to be a natural alternative for the prevention/control of AFB. PMID:23220240

Boligon, Aline Augusti; Brum, Thiele Faccim de; Zadra, Marina; Piana, Mariana; Alves, Camilla Filippi Dos Santos; Fausto, Viviane Pedroso; Júnior, Valdir Dos Santos Barboza; Vaucher, Rodrigo de Almeida; Santos, Roberto Christ Vianna; Athayde, Margareth Linde

2013-02-01

7

Berberis alkaloids. XV. Structure of bargustanine  

Microsoft Academic Search

Bargustanine (I), belonging to a new type of isoquinoline alkaloids, has been isolated from the phenolic fraction of the total alkaloids from the roots ofBerberis vulgaris L. Its structure has been established by chemical and spectral methods.

A. Karimov; M. M. Yusupov; R. Shakirov

1993-01-01

8

Zur Morphologie des Gynözeums von Berberis  

Microsoft Academic Search

Zusammenfassung An Hand abnorm dimerer Gynözeen vonBerberis vulgaris wird die Frage nach einer möglichen Pseudomonomerie desBerberis-Pistills neuerlich aufgeworfen. Der hier mögliche Vergleich zwischen sicheren Einzelkarpellen mit dem normalen Pistill ergibt aber einwandfrei, daß in beiden Organen die Bündel einander vollkommen entsprechen, daß also im normalen Gynözeum keine Restbündel eventuell eingeschmolzener Karpelle vorhanden sind und daß dieses Gynözeum somit echt monomer

Walter Leinfellner

1957-01-01

9

LAM (Lymphangioleiomyomatosis)  

MedlinePLUS

... between the lung and chest wall (the pleural space). A collapsed lung can cause pain and shortness of breath. Sometimes one lung will collapse over and over again. Pneumothorax is a serious condition. It usually requires treatment and might be life threatening. Normal Lungs and Lungs With LAM Figure ...

10

Effect of Scutia buxifolia Reissek in nucleotidase activities and inhibition of platelet aggregation.  

PubMed

This study aimed to determine the effect of lyophilized aqueous extracts of Scutia buxifolia on NTPDase and 5'-nucleotidase activity on platelets and lymphocytes as well as the profile of the platelet aggregation. In vitro tests were used to investigate the effect of the aqueous crude extract obtained from S. buxifolia leaves (SbL) and stem bark (SbS) on enzymatic activities and platelet aggregation. The platelets and lymphocytes were exposed to lyophilized aqueous extracts of S. buxifolia at concentrations of 1-200 ?g/mL in the presence of ATP, ADP, AMP as substrates, during the enzymatic assay, as well as the platelet aggregation. The results showed that SbS and SbL potently inhibited NTPDase and 5'-nucleotidase in platelets and lymphocytes. ADP-induced aggregation was inhibited by the SbS (50, 100, and 200 ?g/mL) and SbL (200 ?g/mL). In addition, these results suggest that S. buxifolia have compounds, such as gallic acid, chlorogenic acid, caffeic acid, quercetin, rutin, and kaempferol, which cause a decrease the NTPDase and 5'-nucleotidase activity, resulting in alterations in adenine nucleotide levels and protection against ADP-induced platelet aggregation. PMID:25149058

Boligon, Aline Augusti; Pimentel, Victor Camera; Bagatini, Margarete Dulce; Athayde, Margareth Linde

2015-01-01

11

Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat  

Microsoft Academic Search

Antioxidant activities of the ethanolic extracts of roots, twigs and leaves of common barberry (Berberis vulgaris L.) and Croatian barberry (Berberis croatica Horvat) were studied. All the extracts were found to possess some radical-scavenging and antioxidant activities, as determined by scavenging effect on the DPPH free radical, reducing power and ?-carotene–linoleic acid model system. With the exception of the ?-carotene–linoleic

M. Zovko Kon?i?; D. Kremer; K. Karlovi?; I. Kosalec

2010-01-01

12

Phytochemistry and Pharmacology of Berberis Species  

PubMed Central

The genus Berberis (Berberidaceae) includes about 500 species worldwide, some of which are widely cultivated in the north-eastern regions of Iran. This genus consists of spiny deciduous evergreen shrubs, characterized by yellow wood and flowers. The cultivation of seedless barberry in South Khorasan goes back to two hundred years ago. Medicinal properties for all parts of these plants have been reported, including: Antimicrobial, antiemetic, antipyretic, antioxidant, anti-inflammatory, anti-arrhythmic, sedative, anti-cholinergic, cholagogic, anti-leishmaniasis, and anti-malaria. The main compounds found in various species of Berberis, are berberine and berbamine. Phytochemical analysis of various species of this genus revealed the presence of alkaloids, tannins, phenolic compounds, sterols and triterpenes. Although there are some review articles on Berberis vulgaris (as the most applied species), there is no review on the phytochemical and pharmacological activities of other well-known species of the genus Berberis. For this reason, the present review mainly focused on the diverse secondary metabolites of various species of this genus and the considerable pharmacological and biological activities together with a concise story of the botany and cultivation. PMID:24600191

Mokhber-Dezfuli, Najmeh; Saeidnia, Soodabeh; Gohari, Ahmad Reza; Kurepaz-Mahmoodabadi, Mahdieh

2014-01-01

13

Phytochemistry and pharmacology of berberis species.  

PubMed

The genus Berberis (Berberidaceae) includes about 500 species worldwide, some of which are widely cultivated in the north-eastern regions of Iran. This genus consists of spiny deciduous evergreen shrubs, characterized by yellow wood and flowers. The cultivation of seedless barberry in South Khorasan goes back to two hundred years ago. Medicinal properties for all parts of these plants have been reported, including: Antimicrobial, antiemetic, antipyretic, antioxidant, anti-inflammatory, anti-arrhythmic, sedative, anti-cholinergic, cholagogic, anti-leishmaniasis, and anti-malaria. The main compounds found in various species of Berberis, are berberine and berbamine. Phytochemical analysis of various species of this genus revealed the presence of alkaloids, tannins, phenolic compounds, sterols and triterpenes. Although there are some review articles on Berberis vulgaris (as the most applied species), there is no review on the phytochemical and pharmacological activities of other well-known species of the genus Berberis. For this reason, the present review mainly focused on the diverse secondary metabolites of various species of this genus and the considerable pharmacological and biological activities together with a concise story of the botany and cultivation. PMID:24600191

Mokhber-Dezfuli, Najmeh; Saeidnia, Soodabeh; Gohari, Ahmad Reza; Kurepaz-Mahmoodabadi, Mahdieh

2014-01-01

14

Assessment of Antioxidant Potential, Total Phenolics and Flavonoids of Different Solvent Fractions of Monotheca Buxifolia Fruit  

PubMed Central

Objectives This study was conducted to investigate the antioxidant potential of methanol extract and its derived fractions (hexane, ethyl acetate, butanol, and aqueous) of fruits of Monotheca buxifolia (Falc.) Dc., a locally used fruit in Pakistan. Methods Dried powder of the fruit of M. buxifolia was extracted with methanol and the resultant was fractionated with solvents having escalating polarity; n-hexane, chloroform, ethyl acetate, n-butanol and the residual soluble aqueous fraction. Total phenolic and total flavonoid contents were estimated for the methanol and various fractions. These fractions were also subjected to various in vitro assays to estimate the scavenging activity for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide, hydroxyl, hydrogen peroxide and reductive ability for ferric ions and phosphomolybdate assay. Results The n-butanol, aqueous and methanol fractions possessed high amount of phenolics and flavonoids compared with other fractions, and subsequently showed a pronounced scavenging activity on DPPH, ABTS, superoxide, hydroxyl and hydrogen peroxide radicals and had a potent reductive ability on ferric ion and phosphomolybdate assay. There was a found significant correlation between total phenolic and flavonoid contents and EC50 of DPPH, superoxide, hydrogen peroxide radical and phosphomolybdate assays, whereas a nonsignificant correlation was found with the hydroxyl radical and ABTS radical assay. Conclusion M. buxifolia fruit can be used as natural antioxidant source to prevent damage associated with free radicals. PMID:24298440

Jan, Shumaila; Khan, Muhammad Rashid; Rashid, Umbreen; Bokhari, Jasia

2013-01-01

15

Phytochemical analysis and toxicity investigation of stem bark of Scutia buxifolia Reissek.  

PubMed

Phytochemical analysis of lyophilised aqueous extract of the stem bark of Scutia buxifolia (SBSB) was carried out by determining total phenolics (0.280 ± 0.02 mg of gallic acid equivalents/g of extract), flavonoids (17.42 ± 2.95 mg of quercetin equivalents/g of extract) and tannins (1.28 ± 0.15 mg of catechin equivalents/g of extract) contents followed by a high-performance liquid chromatography/diode array detection (HPLC/DAD) analysis. The HPLC profile showed caffeic acid, being the major constituent of SBSB (247.21 ± 2.17 mg g?¹ of extract). The antioxidant scavenging capacity of SBSB was determined by 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant power of SBSB was comparable with that of the antioxidant ascorbic acid. Acute toxicity was assayed in rats whereas catalase activity and malondialdehyde production were determined in rats' liver. The SBSB showed safety in the dose tested. This report is the first realised in animals for S. buxifolia. PMID:23163481

de Freitas, Robson Borba; da Costa Araldi, Isabel Cristina; Boligon, Aline Augusti; de Brum, Thiele Faccin; Rovani, Bruno Tomazele; Piana, Mariana; Zadra, Marina; Athayde, Margareth Linde; de Freitas Bauermann, Liliane

2013-01-01

16

Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat.  

PubMed

Antioxidant activities of the ethanolic extracts of roots, twigs and leaves of common barberry (Berberis vulgaris L.) and Croatian barberry (Berberis croatica Horvat) were studied. All the extracts were found to possess some radical-scavenging and antioxidant activities, as determined by scavenging effect on the DPPH free radical, reducing power and beta-carotene-linoleic acid model system. With the exception of the beta-carotene-linoleic acid test, antioxidant activity correlated well with the content of main plant antioxidants, phenols and flavonols, which suggests an important role of these compounds in overall antioxidant activity of investigated plant organs. The antioxidant activity varied mostly in relation to the organ, while no significant statistically differences were found between B. vulgaris and B. croatica. PMID:20488218

Zovko Konci?, M; Kremer, D; Karlovi?, K; Kosalec, I

2010-01-01

17

How Is LAM Treated?  

MedlinePLUS

... may prescribe calcium and vitamin D supplements. Rapamycin (Sirolimus) Sirolimus was originally developed to prevent the immune system ... and movement of LAM cells. Research suggests that sirolimus may shrink tumors in the kidneys of women ...

18

A pharmacological study on Berberis vulgaris fruit extract  

Microsoft Academic Search

Berberis vulgaris fruit (barberry) is known for its antiarrhythmic and sedative effects in Iranian traditional medicine. The effects of crude aqueous extract of barberry on rat arterial blood pressure and the contractile responses of isolated rat aortic rings and mesenteric bed to phenylephrine were investigated. We also examined effect of the extract on potassium currents recorded from cells in parabrachial

Mohammad Fatehi; Tarek M. Saleh; Zahra Fatehi-Hassanabad; Khadige Farrokhfal; Mostafa Jafarzadeh; Samaneh Davodi

2005-01-01

19

Berberis vulgaris as Growth Promoter in Broiler Chickens  

Microsoft Academic Search

Berberis vulgaris (Zereshk in Persian) is a member of therapeutic plants in herbal medicine. There is evidence that its root contains components, such as berberine, berbamine, culumbamine and berberubine, with a relatively wide spectrum of antimicrobial activity. The aim of the present study was to examine if the plant root has any effect on the growth of broiler chickens. The

2006-01-01

20

Morphological comparison of aeciospores from rust fungi infecting Berberis spp.  

Technology Transfer Automated Retrieval System (TEKTRAN)

Currently there are about 30 different species of rust fungi described that infect Berberis and/or Mahonia, and include both autoecious and heteroecious. Our current work is on the molecular phylogenetic and morphological characterization of macrocyclic, heteroecious rust fungi with cereal and grass...

21

D ETECTING THE INFLUENCE OF ORNAMENTAL BERBERIS THUNBERGII VAR. ATROPURPUREA IN INVASIVE POPULATIONS OF BERBERIS THUNBERGII (BERBERIDACEAE) USING AFLP 1  

Microsoft Academic Search

Japanese barberry ( Berberis thunbergii DC.) is a widespread invasive plant that remains an important landscape shrub repre- sented by ornamental, purple-leaved forms of the botanical variety atropurpurea . These forms differ greatly in appearance from feral plants, bringing into question whether they contribute to invasive populations or whether the invasions represent self-sustain- ing populations derived from the initial introduction

Jessica D. Lubell; Mark H. Brand; Jonathan M. Lehrer; Kent E. Holsinger

22

The Invasion Ecology of Japanese Barberry (Berberis thunbergii) in the New England Landscape  

Microsoft Academic Search

Japanese barberry (Berberis thunbergii) has been characterized as one of the most widely known and planted exotic shrubs in the United States. It was first introduced to the US in the late 1800s. By 1920 the planting of Japanese barberry was encouraged as an ornamental shrub replacing the common barberry (Berberis vulgaris). Japanese barberry began spreading from cultivation in suburban

John A. Silander; Debra M. Klepeis

1999-01-01

23

Using HPLC to Determination the Composition and Antioxidant Activity of Berberis Vulgaris  

Microsoft Academic Search

The Berberis vulgaris (BV) plant has been extensively used as a traditional medicine in some part of Asian region especially in Western Asia. The BV fruits is claimed also to have anti-viral activities, and as a treatment for chronic candidiasis, indigestion and parasites. The present study was conducted to evaluate the antioxidative activity, nutritional and antinutritional composition of Berberis vulgaris

Parichehr Hanachi

24

Identification of Berberis spp. as alternate hosts of Puccinia striiformis f. sp. tritici in China  

Technology Transfer Automated Retrieval System (TEKTRAN)

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an important disease on wheat in China because of high virulence diversity. Since the discovery of sexual stage of P. striiformis on Berberis spp., especially B. chinesensis, our interests focused on identifying Berberis spec...

25

Antioxidant and cytoprotective compounds from Berberis vulgaris (barberry).  

PubMed

Activity-guided fractionation of an EtOAc-soluble partition of the MeOH extract from the root bark of Berberis vulgaris L. (barberry), using a hydroxyl radical-scavenging assay, led to the isolation and identification of three phenolic compounds of a previously known structure, N-(p-trans-coumaroyl)tyramine, cannabisin G and (+/-)-lyoniresinol. Of these, cannabisin G and (+/-)-lyoniresinol exhibited antioxidant activity in this bioassay. Furthermore, it was found that cannabisin G showed cytoprotective activity in cultured MCF-7 cells modulated by hydrogen peroxide. PMID:18389483

Tomosaka, Hideyuki; Chin, Young-Won; Salim, Angela A; Keller, William J; Chai, Heebyung; Kinghorn, A Douglas

2008-07-01

26

Cytotoxic steroids from the trunk of Berberis koreana.  

PubMed

A new steroid, itesmol 3-O-palmitate (1), along with two known steroids were isolated from the trunk of Berberis koreana. The structure of 1 was determined on the basis of spectroscopic analyses involving 2D NMR and chemical reactions. Compound 1 exhibited potential antiproliferative activity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines (respective IC(50) values of 7.41, 9.20, 4.53, and 12.91 µM). PMID:22484929

Kim, Ki Hyun; Choi, Sang Un; Kim, Chung Sub; Lee, Kang Ro

2012-01-01

27

Berbanine: a new isoquinoline-isoquinolone alkaloid from Berberis vulgaris (Berberidaceae).  

PubMed

A new isoquinoline-isoquinolone alkaloid was isolated from the root bark of Berberis vulgaris and named berbanine. The structure was established by spectroscopic methods (including 2D NMR, HR-EI-MS). PMID:23738447

Host'álková, Anna; Novák, Zdenek; Pour, Milan; Jirosová, Anna; Opletal, Lubomír; Kunes, Jirí; Cahliková, Lucie

2013-04-01

28

Staphylococcus aureus MDR efflux pump inhibitors from a Berberis and a Mahonia (sensu strictu) species  

Microsoft Academic Search

Bioactive fractionation, based on multi-drug resistance (MDR) pump inhibition in Staphylococcus aureus, resulted in the isolation of the active inhibitors 5?-methoxyhydnocarpin-D from leaves of Berberis (formerly Mahonia) trifoliolata and pheophorbide a from Berberis fendleri. The hydnocarpin derivative was not found in the latter species. Pheophytin a (the phytol derivative of pheophorbide a) was identified from both species, but it proved

Frank R. Stermitz; Teresa D. Beeson; Paul J. Mueller; Jen-Fang Hsiang; Kim Lewis

2001-01-01

29

Detecting the influence of ornamental Berberis thunbergii var. atropurpurea in invasive populations of Berberis thunbergii (Berberidaceae) using AFLP1.  

PubMed

Japanese barberry (Berberis thunbergii DC.) is a widespread invasive plant that remains an important landscape shrub represented by ornamental, purple-leaved forms of the botanical variety atropurpurea. These forms differ greatly in appearance from feral plants, bringing into question whether they contribute to invasive populations or whether the invasions represent self-sustaining populations derived from the initial introduction of the species in the late 19th century. In this study we used amplified fragment length polymorphism (AFLP) markers to determine whether genetic contributions from B. t. var. atropurpurea are found within naturalized Japanese barberry populations in southern New England. Bayesian clustering of AFLP genotypes and principal coordinate analysis distinguished B. t. var. atropurpurea genotypes from 85 plants representing five invasive populations. While a single feral plant resembled B. t. var. atropurpurea phenotypically and fell within the same genetic cluster, all other naturalized plants sampled were genetically distinct from the purple-leaved genotypes. Seven plants from two different sites possessed morphology consistent with Berberis vulgaris (common barberry) or B. ×ottawensis (B. thunbergii × B. vulgaris). Genetic analysis placed these plants in two clusters separate from B. thunbergii. Although the Bayesian analysis indicated some introgression of B. t. var. atropurpurea and B. vulgaris, these genotypes have had limited influence on extant feral populations of B. thunbergii. PMID:21632395

Lubell, Jessica D; Brand, Mark H; Lehrer, Jonathan M; Holsinger, Kent E

2008-06-01

30

Berberis vulgaris root bark extract prevents hyperoxaluria induced urolithiasis in rats.  

PubMed

Berberis vulgaris is a widely used plant for the treatment of urolithiasis. To evaluate its antiurolithic potential, the crude aqueous-methanol extract of Berberis vulgaris root bark (Bv.Cr) was tested in an animal model of urolithiasis, developed in male Wistar rats by adding 0.75% ethylene glycol in drinking water. Bv.Cr (50 mg/kg) inhibited CaOx crystal deposition in renal tubules and protected against associated changes including polyuria, weight loss, impaired renal function and the development of oxidative stress in kidneys. Activity-guided fractionation revealed the concentration of antiurolithic constituent(s) mainly in the aqueous fraction. These data, indicating the presence of antiurolithic activity in Berberis vulgaris root bark, rationalize its medicinal use for the treatment of urolithiasis. PMID:20564494

Bashir, Samra; Gilani, Anwar H; Siddiqui, Anwar A; Pervez, Shahid; Khan, Saeed R; Sarfaraz, Noor Jehan; Shah, Abdul Jabbar

2010-08-01

31

Staphylococcus aureus MDR efflux pump inhibitors from a Berberis and a Mahonia (sensu strictu) species.  

PubMed

Bioactive fractionation, based on multi-drug resistance (MDR) pump inhibition in Staphylococcus aureus, resulted in the isolation of the active inhibitors 5'-methoxyhydnocarpin-D from leaves of Berberis (formerly Mahonia) trifoliolata and pheophorbide a from Berberis fendleri. The hydnocarpin derivative was not found in the latter species. Pheophytin a (the phytol derivative of pheophorbide a) was identified from both species, but it proved to have no MDR pump inhibitory activity. The somewhat uncommon, and inactive, flavonoid tricin was identified from B. trifoliolata. The occurrence of a flavonolignan in Mahonia-tpe species and its absence in Berberis sensu strictu may provide a chemical differentiation between the two groups which are now recombined on the basis of DNA studies. The strong bacterial efflux pump inhibition of pheophorbide a could be of importance as a plant defense against natural pathogens. PMID:11412952

Stermitz, F R.; Beeson, T D.; Mueller, P J.; Hsiang, J -F.; Lewis, K

2001-08-01

32

Investigations into Seed Dormancy in Grevillea linearifolia, G. buxifolia and G. sericea: Anatomy and Histochemistry of the Seed Coat  

PubMed Central

• Background and Aims Seeds of east Australian Grevillea species generally recruit post-fire; previous work showed that the seed coat was the controller of dormancy in Grevillea linearifolia. Former studies on seed development in Grevillea have concentrated on embryology, with little information that would allow testing of hypotheses about the breaking of dormancy by fire-related cues. Our aim was to investigate structural and chemical characteristics of the seed coat that may be related to dormancy for three Grevillea species. • Methods Seeds of Grevillea linearifolia, Grevillea buxifolia and Grevillea sericea were investigated using gross dissection, thin sectioning and histochemical staining. Water movement across the seed coat was tested for by determining the water content of embryos from imbibed and dry seeds of G. sericea. Penetration of intact seeds by Lucifer Yellow was used to test for internal barriers to diffusion of high-molecular-weight compounds. • Key Results Two integuments were present in the seed coat: an outer testa, with exo-, meso- and endotestal (palisade) layers, and an inner tegmen of unlignified sclerenchyma. A hypostase at the chalazal end was a region of structural difference in the seed coat, and differed slightly among the three species. An internal cuticle was found on each side of the sclerenchyma layer. The embryos of imbibed seeds had a water content six times that of dry seeds. Barriers to diffusion of Lucifer Yellow existed at the exotestal and the endotestal/hypostase layers. • Conclusions Several potential mechanisms of seed coat dormancy were identified. The embryo appeared to be completely surrounded by outer and inner barriers to diffusion of high-molecular-weight compounds. Phenolic compounds present in the exotesta could interfere with gas exchange. The sclerenchyma layer, together with strengthening in the endotestal and exotestal cells, could act as a mechanical constraint. PMID:16157632

BRIGGS, C. L.; MORRIS, E. C.; ASHFORD, A. E.

2005-01-01

33

A pharmacological study on Berberis vulgaris fruit extract.  

PubMed

Berberis vulgaris fruit (barberry) is known for its antiarrhythmic and sedative effects in Iranian traditional medicine. The effects of crude aqueous extract of barberry on rat arterial blood pressure and the contractile responses of isolated rat aortic rings and mesenteric bed to phenylephrine were investigated. We also examined effect of the extract on potassium currents recorded from cells in parabrachial nucleus and cerebellum rejoins of rat brain. Administration of the extract (0.05-1 mg/100 g body weight of rat) significantly reduced the mean arterial blood pressure and heart rate in anaesthetized normotensive and desoxycorticosteron acetate-induced hypertensive rats in a dose-dependent manner. Concentration-response curves for phenylephrine effects on isolated rat aortic rings and the isolated mesenteric beds in the presence of the extract were significantly shifted to the right. Application of the extract (1-50 microg/ml) shifted the activation threshold voltage to more negative potentials, leading to an enhancement in magnitude of the outward potassium current recorded from cells present in rat brain slices of parabrachial nucleus and cerebellum. This effect on potassium current may explain the sedative and neuroprotective effects of barberry. The present data support the hypothesis that the aqueous extract of barberry has beneficial effects on both cardiovascular and neural system suggesting a potential use for treatment of hypertension, tachycardia and some neuronal disorders, such as epilepsy and convulsion. PMID:15993555

Fatehi, Mohammad; Saleh, Tarek M; Fatehi-Hassanabad, Zahra; Farrokhfal, Khadige; Jafarzadeh, Mostafa; Davodi, Samaneh

2005-10-31

34

AFLP identification of Berberis thunbergii cultivars, inter-specific hybrids, and their parental species  

Microsoft Academic Search

SUMMARY Cultivars of Berberis thunbergii DC. are among the most popular garden shrubs in the United States. Using 174 amplified fragment length polymorphism (AFLP) markers we were able to differentiate between 43 B. thunbergii cultivars and analyse the genetic similarity of 62 B. thunbergii genotypes, B. julianae, B. koreana, B. vulgaris and B. vulgaris 'Atropurpurea'. Seven different accessions of B.

JESSICA D. LUBELL; MARK H. BRAND; JONATHAN M. LEHRER

2008-01-01

35

Production of Uredia and Telia of Puccinia graminis on Berberis vulgaris  

Microsoft Academic Search

HITHERTO, in Puccinia graminis Pers., the uredial and telial stages have regularly occurred on the gramineous hosts, and the pycnial (spermagonial) and æcial stages on the common barberry (Berberis vulgaris L.) or on closely-related hosts : no deviation from this host relationship has ever been reported. In the course of greenhouse studies, in which barberry plants were infected with pure

Margaret Newton; Thorvaldur Johnson

1937-01-01

36

Treatment of Cutaneous Leishmaniasis in Murine Model by Alcoholic Extract of Berberis vulgaris  

Microsoft Academic Search

In order to evaluate the effect of Berberis vulgarisextract on the experimental ulcers of cutaneous leishmani- asis (CL) on Balb\\/c mice, a study was undertaken over a 12 months period. Forty Balb\\/c mice were divided into 2 main groups A and B. Each main group in turn was divided into 5 sub groups of 4 mice and each sub group

A Fata; H Rakhshandeh; F Berenji

2006-01-01

37

Intra- and interpopulation variability and taxonomic status of Berberis croatica Horvat  

Microsoft Academic Search

Morphological and anatomical traits of Berberis croatica Horvat that would facilitate its separation from the two related taxa, B. aetnensis C. Presl and B. vulgaris L., and their ITS (Internal Transcribed Spacers of nuclear ribosomal DNA) relationships were analyzed. Results show that B. croatica differs from the two related taxa in the following characteristics: plant height, blade width, petiole length,

K. Karlovi?; D. Kremer; Z. Liber; Z. šatovi?; I. Vršek

2009-01-01

38

Inhibition and dissolution of calcium oxalate crystals by Berberis Vulgaris-Q and other metabolites  

Microsoft Academic Search

Inhibition and dissolution of calcium oxalate crystals in solutions containing a homoeopathic medicine Berberis Vulgaris-Q, amino acids such as aspartic acid, glutamic acid, ?-keto glutaric acid, a naturally occurring inhibitor and juices of some fruits of citrus group such as lemon, orange and mausammi have been studied. Considerable solubility was observed and found that in each case solubility changes with

Ishwar Das; S. K Gupta; V. N Pandey; Shoeb A Ansari

2004-01-01

39

Insect-mediated reproduction of systemic infections by Puccinia arrhenatheri on Berberis vulgaris  

Microsoft Academic Search

Summary • Witches' brooms on Berberis vulgaris are induced by a systemically infecting rust fungus, Puccinia arrhenatheri. These witches' brooms bear yellow discolored leaves on which the fungus exposes its gametes in a sugary nectar. During the spermatial stage of the fungus the infected leaves emit a strong, flowery scent. • An exclusion-experiment was used to evaluate whether fungal reproductive

Andreas Naef; Bitty A. Roy; Roman Kaiser; Rosmarie Honegger

2002-01-01

40

Mechanism of seedlessness in Iranian seedless barberry ( Berberis vulgaris L. var. asperma)  

Microsoft Academic Search

Species of barberry (Berberis vulgaris L. var. asperma) is cultivated in arid and semi arid areas of Iran (South Khorasan province). It is widely used as a food additive. Fruits of this species are seedless, while wild type barberries produce seeds in the same area. In this study, we investigated the mechanism of seedlessness in seedless barberry by pollen viability

A. Ebadi; M. Rezaei; R. Fatahi

2010-01-01

41

Pronuciferine N-oxide, a proaporphine N-oxide alkaloid from Berberis coletioides.  

PubMed

Pronuciferine N-oxide (1), a proaporphine N-methyl-N-oxide alkaloid, along with the parent alkaloid pronuciferine (2) were isolated from Berberis coletioides. The structure of the new compound was determined by spectroscopic evidence. Compound 1 is the first naturally occurring proaporphinoid alkaloid with an N-oxide functionality. PMID:19469512

Fajardo, Víctor; Araya, Marisel; Cuadra, Pedro; Oyarzun, Alejandra; Gallardo, Amalia; Cueto, Mercedes; Diaz-Marrero, Ana R; Darias, José; Villarroel, Luís; Alvarez, Celina; Mora-Pérez, Yolanda; Joseph-Nathan, Pedro

2009-07-01

42

Flavonols, alkaloids, and antioxidant capacity of edible wild berberis species from patagonia.  

PubMed

There are 20 species of the Berberidaceae family described in Chile, whose fruits are edible and show high anthocyanin and hydroxycinnamic acid levels. Berberis microphylla G. Forst, commonly known as calafate, is the most extensively distributed. Flavonols and alkaloids in seed, pulp, skin, and whole calafate berry extracts and other Berberis were studied using HPLC-DAD-ESI-MS/MS and HPLC with fluorescence detector. Berry samples from different locations in Chilean Patagonia, including different phenological stages, were systematically addressed. Results were compared with other organs of the plant and with other Berberis species. Total flavonol concentration in calafate (n = 65) was 1.33 ± 0.54 ?mol/g. Glycosyl metabolites of quercetin and isorhamnetin were the most abundant. Similar profiles were observed in calafate from distinct locations, but important differences were observed for the other edible Berberis species. Calafate pulp and skin have higher flavonol concentrations than seeds, and the maturation process reduced its levels. TEACCUPRAC and TEACABTS of whole calafate extracts and fractions are also explored. Finally, only berberine was detected in the fruit (0.001%), mainly in seeds. Results contribute to the promotion of this berry as a superfruit from Patagonia. PMID:25495577

Ruiz, Antonieta; Zapata, Moises; Sabando, Constanza; Bustamante, Luis; von Baer, Dietrich; Vergara, Carola; Mardones, Claudia

2014-12-24

43

Detailed pharmacognostical studies on Berberis aristata DC plant  

PubMed Central

Background: Berberis aristata DC (Berberidaceae) commonly known in Hindi as “D?ruhaldi” and “Citra,” is an important medicinal herb native to Northern Himalaya region. The plant is used traditionally in Indian system of medicine as an antibacterial, antiperiodic, antidiarrheal and anticancer and it is also used in the treatment of ophthalmic infections. Its root, stem and leaves also find their use in treatment of various ailments and hence is used extensively in Ayurveda. Materials and Methods: Samples of the whole plants of B. aristata were collected and identified. Hand and microtome sections were taken, stained and mounted and the cell content and cell wall structure were studied according to the method described by Kay and Johansen. Representative sketches were made with the help of camera Lucida. Methods for determining the quantitative values were the same as described elsewhere. For fluorescence analysis, the powder of the root, stem and leaf were examined under ultraviolet light. Total ash, acid insoluble ash and water-soluble ash values and water- and alcohol-soluble extractives were determined. Results: The detailed investigations carried on the pharmacognosy of the root; stem and leaf of B. aristata have brought out some salient diagnostic features, which allow one to differentiate it from other substitutes and or adulterants. The determination of quantitative values, fluorescence analysis and the use of lycopodium spore analysis has specifically contributed to this differentiation. Conclusion: From the foregoing observation on the pharmacognosy of root, stem and leaf of B. aristata DC, the salient diagnostic characters of three parts have been presented, which can allow one to differentiate it from other substitutes and or adulterants. PMID:24991073

Rathi, Bhawana; Sahu, Juhi; Koul, Sameksha; Kosha, R. L.

2013-01-01

44

Effect of external calcium on the control of stamen movement in Berberis vulgaris L  

Microsoft Academic Search

The mechanical stimulation of the sensitive internal lower part of Berberis vulgaris stamen resulted in its rapid bending.\\u000a In the present study we have examined the influence of external Ca2+ concentration on stamen movement. The external Ca2+ reduced the extent of the response and the effect was dependent on Ca2+ concentration and duration of the treatment. Addition of calcium ionophore

Z. Lechowski; J. Bia?czyk

1992-01-01

45

Antihistaminic and anticholinergic activity of barberry fruit ( Berberis vulgaris) in the guinea-pig ileum  

Microsoft Academic Search

Barberry (Berberis vulgaris) is a well known medicinal plant in Iran and has also been used as food. The antihistaminic and anticholinergic activity of aqueous extract of barberry fruits were investigated on isolated guinea-pig ileum, and dose–response curves of histamine and acetylcholine with and without extract were plotted. The pA2 values for antihistaminic activity of extract and dexchlorpheniramine were calculated

F Shamsa; A Ahmadiani; R Khosrokhavar

1999-01-01

46

Inhibition and dissolution of calcium oxalate crystals by Berberis Vulgaris-Q and other metabolites  

NASA Astrophysics Data System (ADS)

Inhibition and dissolution of calcium oxalate crystals in solutions containing a homoeopathic medicine Berberis Vulgaris-Q, amino acids such as aspartic acid, glutamic acid, ?-keto glutaric acid, a naturally occurring inhibitor and juices of some fruits of citrus group such as lemon, orange and mausammi have been studied. Considerable solubility was observed and found that in each case solubility changes with time or concentration periodically. Chelation may be one of the reasons for inhibition.

Das, Ishwar; Gupta, S. K.; Pandey, V. N.; Ansari, Shoeb A.

2004-07-01

47

Berberis Fruit Extract and Biochemical Parameters in Patients With Type II Diabetes  

PubMed Central

Background: Diabetes mellitus is a common medical problem. There is in fact a growing body of literature on plants used for the treatment of diabetes. Plant materials attracted considerable interest of scientists. In this respect, in the past few years, attempts were made to use natural plant products for the treatment of patients with diabetes. Objectives: The aim of this study was to investigate the effect of Berberis fruit extract to achieve glycemic control in patients with Type II diabetes. Materials and Methods: This study was performed between July 2010 and April 2013. Thirty patients of type II diabetes admitted to Ayatollah Rohhani hospital were recruited. Patients’ sera were collected for the assessment of glucose and HbA1c values. Biochemical analyses were performed before and after treatment by Berberis fruit extract. Biochemical parameters were measured by spectrophotometric method (Jenway uv/vis, 6505 model, Dunmow, UK). Glucose level was measured by glucose oxidase method kit (Pars Azmoon, Tehran, IR Iran). Serum total cholesterol and triglycerides were measured using standard biochemical kits (Pars Azmoon, Tehran, Iran). Blood glycated hemoglobin level was measured by using Elisa kit (Bioassay technology laboratory, Elisa kit). Experiments were performed in triplicate in at least three separate experiments. Results: Our findings demonstrated that patients with type 2 diabetes who received barberry fruit had significant reduction in serum glucose to 136.15 ± 32.8 mg/dL and decreased HbA1c levels to 7.07 ± 1.21 mg/dL, during the 8 weeks of study. Conclusions: This investigation revealed that Berberis fruit extract has beneficial metabolic effects in patients with type II diabetes. Barberry may improve glucose catabolism via glycolysis pathway, stimulate insulin secretion or improve insulin function and finally decrease glucose uptake. Our results indicated that Berberis fruit regulates glucose metabolism in patients with type 2 diabetes. PMID:24872938

Moazezi, Zolikha; Qujeq, Durdi

2014-01-01

48

Effects of Berberis aristata/Silybum marianum association on metabolic parameters and adipocytokines in overweight dyslipidemic patients.  

PubMed

Nutraceuticals and functional foods have attracted considerable interest as potential alternative therapies for treatment of different cardiovascular disorders and insulin resistance. We evaluated the efficacy of a combination of Berberis Aristata/Silybum Marianum extract (Berberol®) in a sample of overweight, dyslipidemic patients at low cardiovascular risk. We enrolled 105 Caucasian, euglycemic, overweight, dyslipidemic patients, of either sex. At baseline all patients underwent a 6 months run-in period during which they followed an adequate diet and practiced physical activity. At the end of the run-in period, patients were randomised to take placebo or a combination of Berberis aristata/Silybum marianum, 1 tablet during the lunch and 1 tablet during the dinner, for three months, in a double-blind, placebo-controlled design. Berberis aristata/Silybum marianum and placebo were then interrupted for 2 months (wash-out period), and all patients continued with only diet and physical activity. At the end of the wash-out period, patients re-started Berberis aristata/Silybum marianum or placebo twice a day for further 3 months. We evaluated during the run-in period, at randomisation, before and after the wash-out period these parameters: body weight and BMI, fasting plasma glucose, lipid profile, insulin resistance, retinol binding protein-4 (RBP-4), adiponectin (ADN), resistin. Total cholesterol, LDL-C, and Tg decreased, and HDL-C increase after 3 months of Berberis aristata/Silybum marianum, both compared to baseline and placebo. Berberis aristata/Silybum marianum decreased fasting plasma insulin, and HOMA-IR, both compared to baseline and to placebo. Moreover, there was a decrease of RBP-4, and resistin, and an increase of ADN after 3 months of Berberis aristata/Silybum marianum. All these positive effects disappeared after the wash-out period, and re-appeared after the re-introduction of the drug. We observed a significant correlation between HOMA-index decrease and resistin, and RBP-4 decrease, and between HOMA-index decrease and ADN increase in Berberis aristata/Silybum marianum group, but not in placebo group. Berberis aristata/Silybum marianum fixed combination seems to be safe and effective in improving lipid profile, but also in improving insulin resistance and adipocytokines levels. PMID:24152839

Derosa, G; Bonaventura, A; Bianchi, L; Romano, D; D' Angelo, A; Fogari, E; Maffioli, P

2013-01-01

49

Universal Plant DNA Barcode Loci May Not Work in Complex Groups: A Case Study with Indian Berberis Species  

PubMed Central

Background The concept of DNA barcoding for species identification has gained considerable momentum in animals because of fairly successful species identification using cytochrome oxidase I (COI). In plants, matK and rbcL have been proposed as standard barcodes. However, barcoding in complex genera is a challenging task. Methodology and Principal Findings We investigated the species discriminatory power of four reportedly most promising plant DNA barcoding loci (one from nuclear genome- ITS, and three from plastid genome- trnH-psbA, rbcL and matK) in species of Indian Berberis L. (Berberidaceae) and two other genera, Ficus L. (Moraceae) and Gossypium L. (Malvaceae). Berberis species were delineated using morphological characters. These characters resulted in a well resolved species tree. Applying both nucleotide distance and nucleotide character-based approaches, we found that none of the loci, either singly or in combinations, could discriminate the species of Berberis. ITS resolved all the tested species of Ficus and Gossypium and trnH-psbA resolved 82% of the tested species in Ficus. The highly regarded matK and rbcL could not resolve all the species. Finally, we employed amplified fragment length polymorphism test in species of Berberis to determine their relationships. Using ten primer pair combinations in AFLP, the data demonstrated incomplete species resolution. Further, AFLP analysis showed that there was a tendency of the Berberis accessions to cluster according to their geographic origin rather than species affiliation. Conclusions/Significance We reconfirm the earlier reports that the concept of universal barcode in plants may not work in a number of genera. Our results also suggest that the matK and rbcL, recommended as universal barcode loci for plants, may not work in all the genera of land plants. Morphological, geographical and molecular data analyses of Indian species of Berberis suggest probable reticulate evolution and thus barcode markers may not work in this case. PMID:21060687

Roy, Sribash; Tyagi, Antariksh; Shukla, Virendra; Kumar, Anil; Singh, Uma M.; Chaudhary, Lal Babu; Datt, Bhaskar; Bag, Sumit K.; Singh, Pradhyumna K.; Nair, Narayanan K.; Husain, Tariq; Tuli, Rakesh

2010-01-01

50

A new isoquinoline alkaloid with anti-microbial properties from Berberis jaeschkeana Schneid. var. jaeschkeana.  

PubMed

One new isoquinoline alkaloid named berberidione (1) along with four new source alkaloids berberine (2), palmatine (3), jatrorrhizine (4) and chondrofoline (5) and three new source non-alkaloids syringic acid (6), ?-sitosterol (7) and stigmasterol (8) was isolated and characterised from different fractions of Berberis jaeschkeana Schneid var. jaeschkeana. All the structures were determined from 1D and 2D spectroscopic data. Crude extract, sub-fractions and isolated compounds showed excellent anti-microbial properties. The toxicity level for the alkaloids was found to be very low on THP-1 cells. PMID:25424893

Alamzeb, Muhammad; Khan, M Rafiullah; Ali, Saqib; Khan, Ashfaq Ahmad

2015-04-01

51

Role of Berberis spp. as alternate hosts in generating new races of Puccinia graminis and P. striiformis  

Microsoft Academic Search

The common barberry and several other Berberis spp. serve as the alternate hosts to two important rust pathogens of small grains and grasses, Puccinia graminis and P. striiformis. Barberry eradication has been practiced for centuries as a means to control stem rust. Diverse virulence variations have\\u000a been observed in populations of P. graminis f. sp. tritici that were associated with

Yue Jin

2011-01-01

52

The Effect of Light, Temperature, pH and Species on Stability of Anthocyanin Pigments in Four Berberis Species  

Microsoft Academic Search

2 Abstract: The anthocyanin pigment was extracted from the four different Berberis plant (B. khorasanica, B. integerrima, B. orthobotrys, and B. vulgaris) using the soaking and wetting in Ethanol (1% acidified). The extracted anthocyanin pigments then were exposed to number of environmental conditions, which could destabilize the anthocyanin molecules. These environmental conditions were included three different pHs (0, 1.5 and

2006-01-01

53

Century-old Mystery of Puccinia striiformis Life History Solved with the Identification of Berberis as an Alternate Host  

Technology Transfer Automated Retrieval System (TEKTRAN)

The life history of Puccinia striiformis remains a mystery because the alternate host has never been found. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P...

54

“Getting stuck with LAM”: patients perspectives on living with Lymphangioleiomyomatosis  

PubMed Central

Background Lymphangioleiomyomatosis (LAM) is a rare, progressive lung disease that affects almost exclusively women and is most often diagnosed before menopause. The main symptom of LAM is shortness of breath. LAM patients’ perceptions of how the disease impacts their lives is largely unknown, but such information could be useful to generate patient reported outcome measures for use in drug trials (or other research studies) and to formulate interventions aimed at easing the burdens LAM imposes on patients. Objective To capture patients’ perceptions of how LAM affects their lives. Methods We used reflexive team analysis to analyze transcripts from semi-structured focus groups conducted with LAM patients at LAMposium 2013. We sought to determine what patients perceive as the primary symptoms of LAM and how the disease affects them in their daily lives. Results The 37 participants described seven primary symptoms of LAM and five common psychological experiences from living with the disease. Shortness of breath and low energy (or fatigue) dominated the symptomatic picture; cough, sensations in the chest, difficulty sleeping, gastrointestinal issues, and mild cognitive difficulties were less common. The common psychological experiences participants reported included frustration, worry, loss of identity, embarrassment, and in some participants, a healthy defiance against the disease. Conclusions Patients perceive the physical symptoms from LAM to be intrusive and limiting. Women living with LAM are frustrated by their physical limitations, and they worry about what the future will be like if the disease progresses. Therapeutic interventions should take aim at improving these perceptions. PMID:24885591

2014-01-01

55

Aqueous extract of Berberis integerrima root improves renal dysfunction in streptozotocin induced diabetic rats  

PubMed Central

Objective: Barberry root extract contains various alkaloids that are considered as antioxidants. Beneficial effect of aqueous extract of Berberis integerrima root (AEBIR) was evaluated for renal function in diabetic rats induced by STZ. Material and Methods: Diabetes was induced by i.p. injection of streptozotocin (65 mg/kg bw) to rats, after 15 h of fasting. Diabetic rats were randomly grouped and treated daily with AEBIR and glibenclamide by gavage for 42 days. After 6 weeks of study, all the rats were sacrificed and some biochemical parameters of serum and urine were measured and their kidneys tissues were processed for light microscopy. Results: Streptozotocin induced a significant rise in fasting blood glucose, serum creatinine, blood urea nitrogen, urine glucose, urine protein, urine albumin, and water intake and a significant decrease in body weight, serum protein, urine urea, and urine creatinine. There was a significant restoration of these parameters to near normal after administration of the AEBIR and also by glibenclamide (0.6 mg/kg bw). The activity of the extract at dose of 500 mg/kg in all parameters except blood glucose and urine glucose was more than that of the standard drug, glibenclamide (0.6 mg/kg, p.o.). Histopathological changes of kidney samples were comparable with respective control. Conclusion: These results suggested that aqueous extract of Berberis Integerrima root improves renal dysfunction in streptozotocin-induced diabetic rats through controlling blood glucose and renal protective effects. PMID:25050261

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid; Ilkhanipoor, Minoo

2013-01-01

56

On LAM's and SAM's for Halley's rotation  

NASA Technical Reports Server (NTRS)

Non principal axis rotation for comet Halley is inferred from dual periodicities evident in the observations. The modes where the spin axis precesses around the axis of minimum moment of inertia (long axis mode or LAM) and where it precesses around the axis of maximum moment of inertia (short axis mode or SAM) are described from an inertial point of view. The currently favored LAM model for Halley's rotation state satisfies observational and dynamical constraints that apparently no SAM can satisfy. But it cannot reproduce the observed post perihelion brightening through seasonal illumination of localized sources on the nucleus, whereas a SAM can easily produce post or pre perihelion brightening by this mechanism. However, the likelihood of a LAM rotation for elongated nuclei of periodic comets such as Halley together with Halley's extreme post perihelion behavior far from the Sun suggest that Halley's post perihelion brightening may be due to effects other than seasonal illumination of localized sources, and therefore such brightening may not constrain its rotation state.

Peale, Stanton J.

1992-01-01

57

The Effect of Berberis Vulgaris Extract on Transaminase Activities in Non-Alcoholic Fatty Liver Disease  

PubMed Central

Background: Non-alcoholic fatty liver disease (NAFLD) is the most prevalent liver disorder in western countries and an important cause of liver cirrhosis, as well as liver failure. Up to now, 20?40% of the individuals suffer from this disorder and its prevalence is estimated around 5?30% in Asia. The NAFLD is one of the most prevalent causes for increases in liver enzymes and has a close relationship with obesity, dyslipidemia, hypertension, and type II diabetes. However, no definite treatment has been identified for it yet. Objectives: The present study aimed to investigate the effect of berberis vulgaris extract in inducing changes in liver enzymes levels. Patients and Methods: The present clinical trial was conducted on 80 patients, including 32 males (40%) and 48 females (60%), who were randomly assigned into two groups of case and control. All the patients had ultrasound evidence of lipid accumulation in the liver and increases in liver enzymes. The case group received two capsules (750 mg) containing berberis vulgaris extract every day for 3 months, while the control group was treated with placebo. The weight, liver transaminases levels and lipid profiles of the two groups were assessed before, during, and after the study. Results: In the case group, the mean serum levels of alanine transaminase (ALT) and aspartate transaminase (AST) decreased from 49 to 27.48 and 48.22 to 29.8 u/L, respectively, which was statistically significant compared to the control group (P < 0.001, P < 0.001). In the control group, the mean of ALT and AST decreased from 50.4 to 46.8 and 45.7 to 44.9 u/L, respectively. The difference was not statistically significant. In addition, a significant decrease was observed in weight, triglycerides, and cholesterol, while no significant change was found in fasting blood sugar, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL). Conclusions: Considering the significant decrease in the liver enzymes, triglycerides and cholesterol after using berberis vulgaris extract, further studies with larger sample sizes will identify the accurate dose as well as duration of consumption for this extract, to recommend in the treatment of patients with NAFLD. PMID:25788958

Iloon Kashkooli, Raziye; Najafi, Seyed Saeed; Sharif, Farkhondeh; Hamedi, Azadeh; Hoseini Asl, Mohammad Kazem; Najafi Kalyani, Majid; Birjandi, Mehdi

2015-01-01

58

Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine.  

PubMed

Barberry (Berberis vulgaris L. family Berberidaceae) is well known in Iran and various parts of this plant including its root, bark, leaf and fruit have been used as folk medicine. The two decades of research has demonstrated different pharmacological and therapeutic effects of B. vulgaris and its isoquinoline alkaloids (particularly berberine). Studies carried out on the chemical composition of the plant show that the most important constituents of this plant are isoquinoline alkaloids such as berberine, berbamine and palmatine. Berberine represents one of the most studied among the naturally occurring protoberberine alkaloids. In addition to B. vulgaris (barberry), berberine is present in many other plants and is used for the treatment of different diseases. This article reviews the traditional uses and pharmacological effects of total extract and the most active ingredient of B. vulgaris (berberine). PMID:18618524

Imanshahidi, Mohsen; Hosseinzadeh, Hossein

2008-08-01

59

Windows of opportunity: historical and ecological controls on Berberis thunbergii invasions.  

PubMed

Attempts to determine characteristics that render habitats invasible to nonnative species have met with limited success. This may be because most studies focus on modern habitat conditions and do not consider invasibility in the context of a historically dynamic landscape in which both the abundance of a species and the invasibility of a site may change. We surveyed 159 currently forested sites for the occurrence and abundance of Berberis thunbergii (Japanese barberry), an invasive, nonnative shrub in forests of the northeastern United States, relative to modern environmental conditions, contemporary logging activity, and two periods of historical land use. Berberis thunbergii occurred more frequently and was more abundant in post-agricultural forests than in continuously wooded sites. This relationship was stronger for agricultural sites that were abandoned and reforested after B. thunbergii was introduced to the region than for sites that reforested prior to B. thunbergii introduction. In contrast, recent forest harvesting did not influence the occurrence or abundance of B. thunbergii. Modern soil fertility explained a significant portion of the variation in B. thunbergii occurrence, whereas site history considerably improved predictions of population density and helped evaluate potential invasion mechanisms. While land-use history covaries with soil fertility and distance to putative seed sources, the strong relationship between modern abundance patterns and historical agriculture suggests that B. thunbergii colonized recently abandoned agricultural lands in the early 20th century and then persisted and spread locally during subsequent reforestation. Our results indicate that interpretations of both native community composition and modern plant invasions must consider the importance of historical landscape changes and the timing of species introduction along with current environmental conditions. PMID:18229845

DeGasperis, Brian G; Motzkin, Glenn

2007-12-01

60

Rehabilitation program faculty mem-bers Chow Lam, Patrick Corrigan,  

E-print Network

Rehabilitation program faculty mem- bers Chow Lam, Patrick Corrigan, and Jon Larson, and Institute in rehabilitation and education. There are very few universities in China that offer studies on rehabili- tation psychology, and Lam believes IIT's MOU with CAS and his efforts in training future rehabilitation pro

Heller, Barbara

61

Effects of Aqueous Extract of Berberis integerrima Root on Some Physiological Parameters in Streptozotocin-Induced Diabetic Rats.  

PubMed

Diabetes mellitus is a common endocrine disorder. Anti-diabetic agents from natural and synthetic sources are available for the treatment of this disease. Berberis integerrima is a medicinal shrub used in conventional therapy for a number of diseases. The aim of the present study was to investigate the effects of aqueous extract of Berberis integerrima root (AEBI) on some physiological parameters in normal and streptozotocin-induced (STZ-induced) diabetic male Wistar rats. STZ-induced diabetic rats showed significant increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C), creatinine (Cr), urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin while body weight, high density lipoprotein HDL-cholesterol (HDL-C) and total protein levels were significantly decreased compared to normal rats. Treatment of diabetic rats with different doses of aqueous extract of Berberis integerrima root (250 and 500 mg/Kg bw) resulted in a significant decrease in blood glucose, triglycerides, cholesterol, LDL-cholesterol, ALT, AST, ALP, total bilirubin, creatinine and urea while HDL-cholesterol and total protein levels were markedly increased after six weeks compared to untreated diabetic rats. The effects of the AEBI at dose of 500 mg/Kg in all parameters except blood glucose (similar) is more than to the standard drug, glibenclamide (0.6 mg/Kg, p.o.). The results of this study indicate that the tested aqueous extract of Berberis integerrima root possesses hypoglycemic, hypolipidemic and antioxidant effects in STZ-induced diabetic rats. PMID:24250618

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid; Ilkhanipoor, Minoo

2013-01-01

62

Effects of Aqueous Extract of Berberis integerrima Root on Some Physiological Parameters in Streptozotocin-Induced Diabetic Rats  

PubMed Central

Diabetes mellitus is a common endocrine disorder. Anti-diabetic agents from natural and synthetic sources are available for the treatment of this disease. Berberis integerrima is a medicinal shrub used in conventional therapy for a number of diseases. The aim of the present study was to investigate the effects of aqueous extract of Berberis integerrima root (AEBI) on some physiological parameters in normal and streptozotocin-induced (STZ-induced) diabetic male Wistar rats. STZ-induced diabetic rats showed significant increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C), creatinine (Cr), urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin while body weight, high density lipoprotein HDL-cholesterol (HDL-C) and total protein levels were significantly decreased compared to normal rats. Treatment of diabetic rats with different doses of aqueous extract of Berberis integerrima root (250 and 500 mg/Kg bw) resulted in a significant decrease in blood glucose, triglycerides, cholesterol, LDL-cholesterol, ALT, AST, ALP, total bilirubin, creatinine and urea while HDL-cholesterol and total protein levels were markedly increased after six weeks compared to untreated diabetic rats. The effects of the AEBI at dose of 500 mg/Kg in all parameters except blood glucose (similar) is more than to the standard drug, glibenclamide (0.6 mg/Kg, p.o.). The results of this study indicate that the tested aqueous extract of Berberis integerrima root possesses hypoglycemic, hypolipidemic and antioxidant effects in STZ-induced diabetic rats. PMID:24250618

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid; Ilkhanipoor, Minoo

2013-01-01

63

Comparative fungal responses in managed plant communities infested by spotted ( Centaurea maculosa Lam.) and diffuse ( C. diffusa Lam.) knapweed  

Microsoft Academic Search

Spotted knapweed (Centaurea maculosa Lam.) and diffuse knapweed (Centaurea diffusa Lam.) infested plant communities that are being studied for improved management practices to enhance native perennial grass species were evaluated for selected fungal responses during 2000–2003. Replicated plots were treated using combinations of carbohydrate addition to reduce soil N availability, reseeding with desirable mid- and late-seral plant species, and reintroducing

Donald A. Klein; Mark W. Paschke; Tamara L. Heskett

2006-01-01

64

Lymphangiogenesis-Mediated Shedding of LAM Cell Clusters as a Mechanism for Dissemination in Lymphangioleiomyomatosis  

Microsoft Academic Search

Lymphangioleiomyomatosis (LAM) affects exclusively women of reproductive age, involves the lungs and axial lymphatic system, and is frequently complicated with renal angiomyolipomas. LAM lesions are generated by the proliferation of LAM cells with mutations of one of the tuberous sclerosis complex (TSC) genes. Recent studies indicate that LAM cells can migrate or metastasize to form new lesions in multiple organs,

Toshio Kumasaka; Kuniaki Seyama; Keiko Mitani; Sanae Souma; Satoko Kashiwagi; Akira Hebisawa; Teruhiko Sato; Hajime Kubo; Kiyoshi Gomi; Kazutoshi Shibuya; Yoshinosuke Fukuchi; Koichi Suda

2005-01-01

65

Antileishmanial compounds from Moringa oleifera Lam.  

PubMed

The antileishmanial activity of extracts and phytoconstituents of Moringa oleifera Lam. was investigated in vitro against promastigotes of Leishmania donavani. The 70% ethanolic extract of roots and the methanolic extract of leaves showed moderate inhibitory activity with IC50 values of 83.0 microg/ml and 47.5 microg/ml, respectively. Antileishmanial activity of the methanolic extract of leaves increased upon fractionation, as its ethyl acetate fraction was found to be more active with an IC50 value of 27.5 microg/ml. The most active antileishmanial compound niazinin, a thiocarbamate glycoside isolated from this fraction, showed an IC50 value of 5.25 microM. Results presented in this study indicate that extracts from M. oleifera may be developed as an adjuvant therapy for the treatment of leishmaniasis. PMID:24873031

Kaur, Amandeep; Kaur, Preet Kamal; Singh, Sushma; Singh, Inder Pal

2014-01-01

66

Classical closure theory and Lam's interpretation of epsilon-RNG  

NASA Technical Reports Server (NTRS)

Lam's phenomenological epsilon-renormalization group (RNG) model is quite different from the other members of that group. It does not make use of the correspondence principle and the epsilon-expansion procedure. We demonstrate that Lam's epsilon-RNG model is essentially the physical space version of the classical closure theory in spectral space and consider the corresponding treatment of the eddy viscosity and energy backscatter.

Zhou, YE

1995-01-01

67

Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum.  

PubMed

Barberry (Berberis vulgaris) is a well known medicinal plant in Iran and has also been used as food. The antihistaminic and anticholinergic activity of aqueous extract of barberry fruits were investigated on isolated guinea-pig ileum, and dose response curves of histamine and acetylcholine with and without extract were plotted. The pA2 values for antihistaminic activity of extract and dexchlorpheniramine were calculated (extract; pA2 +/- S.E.M. = 4.50 +/- 0.01[-log C (g/l)]; dexchlorpheniramine; pA2 +/- S.E.M. = 9.36 +/- 0.14[-log C (M)]) and compared with each other. The pA2 values for anticholinergic activity of extract and atropine were also calculated (extract, pA2 +/- S.E.M. = 4.37 +/- 0.03[-log C (g/l)]; atropine, pA2 +/- S.E.M. = 8.99 +/- 0.13[-log C (M)]) and compared. The results indicated antihistaminic and anticholinergic activity of extract that seems to be of the competitive type. PMID:10197751

Shamsa, F; Ahmadiani, A; Khosrokhavar, R

1999-02-01

68

Spine production is induced by fire: a natural experiment with three Berberis species  

NASA Astrophysics Data System (ADS)

Earlier studies indicate that some plant species allocate more mass to produce longer spines in shoots resprouting after browsing. Here we present, for the first time, evidence that fire induces a similar response. Many terrestrial herbivores may benefit from fire through the enhanced availability of fast growing species colonizing or re-sprouting in burned areas. It is less clear whether post-fire plant growth responds to the enhanced risk of herbivory by an increased investment in defensive traits. In this study, we tested whether the production of spines is influenced by the set of environmental conditions that result from fire events. We compared the resource allocation pattern of resprouting shoots from three Berberis species growing in two areas that burned 1999 with samples collected from unburned areas within the same plant communities. We divided the shoot into three main components: supporting tissue (twigs), assimilating tissue (leaves) and defensive structures (spines). We found that plants resprouting after fire allocated more mass to spines and leaves but not twigs. This resulted in a higher density of both spines and leaves. Spines were significantly longer in plants resprouting after fire. Leaves were shorter at the apical end of the shoot, but did not show any significant change in size following fire. We suggest that this type of post-fire response may be a general adaptation to pruning and leaf picking by browsing herbivores in arid and semi-arid regions. Changes in the browsing pressure following fire will determine the fitness value of this response.

Gowda, Juan; Raffaele, Estela

2004-12-01

69

Possible mechanism of cardiac depressant activity of Berberis orthobotrys roots in isolated rabbit heart.  

PubMed

Berberis orhob, otrvs Bien. ex Aitch. (B.o.) has been reported to have antihypertensive effect in different experimental models. The aim of present study was to evaluate the possible antihypertensive mechanism. Aqueous methanolic extract of B.o. roots and its various fractions namely (ethyl acetate, n-butanol or aqueous) in different concentrations (10 ng/mL, 100 ng/mL, I pg/mL, 10 pg/mL, 100 pg/mL ) were evaluated in isolated perfused rabbit heart to assess their effect on force of contraction, HR and perfusion pressure. The crude extract of B.o. and its fractions exhibited a significant decrease in heart rate, contractility and perfusion pressure of isolated rabbit heart, however, butanolic fraction produced more prominent effect and was selected for further study. The effects of butanol fraction were not blocked by atropine (10' M) in isolated perfused heart. However, butanol fraction significantly blocked the effects of adrenaline (10' M). It is therefore conceivable that cardiac depressant activity of B.o. butanol fraction might be due to the presence of certain 3-blocking agents which might be responsible for antihypertensive effect However, further experiments are required to isolate the active compound(s) and elucidate exact mechanism of action. PMID:25272893

Alamgeer; Akhtar, Muhammad Shoaib; Jabeen, Qaiser; Bashir, Sajid; Malik, Muhammad Nasir Hayat; Karim, Sabeha; Mushtaq, Muhammad Naveed; Rasool, Shahid; Latif, Fozia; Tabbasum, Nazia; Khan, Abdul Qayyum; Ahsan, Haseeb; Khan, Wasim; Javed, Ibrahim

2014-01-01

70

Effect of different extraction protocols on anticancer and antioxidant activities of Berberis koreana bark extracts.  

PubMed

High-pressure extraction and ultrasonification extraction techniques were employed to extract bioactive compounds from Berberis koreana. This study aimed to determine the effect of ultrasonification in a high pressure process on the extraction yield, and the anticancer and antioxidant activities of the B. koreana bark extract. The effect of high-pressure extraction time when carried out for 5 and 15 min (HP5 and HP15) was also investigated. The best extraction yield with maximum percentage of phenolic compounds was obtained using high pressure with sonification (HPWS) extraction method. Experimental results indicated that HPWS altered the antioxidant activities, including the scavenging capacity of diphenylpicrylhydrazyl (DPPH) and xanthine oxidase. HP5 and HP15 with conventional extraction have almost similar bioactivity, but showed lower antioxidant and anticancer activities compared to HPWS. The results showed that the application of ultrasonification improved the extraction efficiency for bioactive compounds and, as deduced from chromatographic profiles, it may have allowed the release of new compounds. The scanning electron microscope (SEM) showed evidence of rupturing of the tissue surface treated with HPWS, in contrast to conventional extraction, HP5, and HP15. The HPWS extraction was not only more efficient but also convenient for the recovery and purification of the active compounds of hard plant tissues. PMID:19269602

Qadir, Syed Abdul; Kwon, Min Chul; Han, Jae Gun; Ha, Ji He; Chung, Hyang Suk; Ahn, Juhee; Lee, Hyeon Yong

2009-03-01

71

5'-Methoxyhydnocarpin-D and pheophorbide A: Berberis species components that potentiate berberine growth inhibition of resistant Staphylococcus aureus.  

PubMed

A new method of bioactivity-directed fractionation, based on multidrug resistant pump (MDR) inhibition in Staphylococcus aureus, was demonstrated. This resulted in the isolation, from berberine-containing Berberis species, of two compounds that are themselves devoid of S. aureus antibacterial activity, but that form potent synergistic couples with a subinhibitory concentration of berberine. The bacterial MDR pump inhibitors were identified as the flavonolignan 2 and the porphyrin 3. Another natural flavonolignan, silybin (8) from Silybum marianum, was also shown to be a bacterial MDR pump inhibitor. PMID:10978214

Stermitz, F R; Tawara-Matsuda, J; Lorenz, P; Mueller, P; Zenewicz, L; Lewis, K

2000-08-01

72

The next breakthrough in LAM clinical trials may be their design: challenges in design and execution of future LAM clinical trials.  

PubMed

The past decade has resulted in stunning progress in the pathogenesis and therapy of lymphangioleiomyomatosis (LAM), culminating in the pivotal 'MILES' trial, the first-ever randomized, placebo-controlled trial in LAM, demonstrating the efficacy of sirolimus in 2011. Here, we review clinical progress since 2011, focusing on new therapeutic and observational trials. These trials include the second randomized, placebo-controlled trial, a 2-year study of doxycycline effectiveness in LAM. Other clinical studies have addressed lower-dose sirolimus and treatment of pulmonary hypertension. An improved understanding of LAM pathogenesis is essential to future therapeutic breakthroughs. Critical questions that remain to be addressed include the role of estrogen and lymphangiogenesis in LAM pathogenesis and therapy, mechanisms of cystic lung destruction, the role of autophagy and pro-survival pathways in LAM cell survival. Ultimately, achieving future 'breakthroughs' in LAM will require continued rigorous basic and preclinical investigation, innovative clinical trial design and robust biomarkers. PMID:25757365

El-Chemaly, Souheil; Henske, Elizabeth P

2015-04-01

73

Aqueous extract of dried fruit of Berberis vulgaris L. in acne vulgaris, a clinical trial.  

PubMed

Berberis vulgaris L. (barberry) is a very well-known herb in traditional medicine. Apart from its anti-inflammatory and antibacterial properties, the antilipogenic effect of barberry on the sebaceous glands in animals may further suggest it could be employed as an anti-acne agent. This study examined the effect of oral aqueous extract of barberry on acne vulgaris. Adolescents aged 12-17 years with moderate to severe acne vulgaris were randomly given oral gelatin capsules containing either aqueous extract of dried barberry (600 mg daily for 4 weeks, n = 25) or placebo (n = 24). Counts of facial noninflamed, inflamed, and total acne lesions, as well as the Michaelson's acne severity score were documented at baseline and at weeks 2 and 4. Both groups were comparable in terms of the patients' characteristics and baseline variables. After 4 weeks, the mean number of noninflamed, inflamed, and total lesions as well as mean Michaelson's acne severity score declined significantly by 43.25 ± 10.88% (median: 42.11%), 44.53 ± 11.78% (median: 45.45%), 44.64 ± 8.46% (median: 46.15%), and 44.38 ± 8.25% (median: 44.07%), respectively, among the extract receivers (p <.001 for all the changes). Similar changes were not significant in the placebo group. No notable complication or side effect was reported in relation to barberry. In conclusion, oral aqueous extract of dried barberry is a safe, well-tolerated, and effective choice in teenagers with moderate to severe acne vulgaris. PMID:23038982

Fouladi, Rohollah F

2012-12-01

74

Sexual structures and recombination of the wheat rust fungus Puccinia striiformis on Berberis vulgaris.  

PubMed

An isolate of the basidiomycete Puccinia striiformis, which causes yellow (stripe) rust on wheat, was selfed on the newly discovered alternate host, Berberis vulgaris. This allowed a study of the segregation of molecular markers and virulence in the progeny isolates, and of the development of fungal sexual structures and spore forms. Pycnia and aecia were obtained after inoculation of B. vulgaris with basidiospores resulting from germinating teliospores from infected wheat leaves. Subsequent inoculation of wheat with aeciospores from bulked aecia resulted in 16 progeny isolates of the S1 generation. Genotyping with 42 simple sequence repeat (SSR) markers confirmed a parental origin of progeny isolates. Of the 42 analyzed loci, 15 were heterozygous in the parental isolate and 14 revealed segregation in the progenies. This resulted in 11 new multilocus genotypes (MLGs), which confirmed segregation following sexual reproduction. Additionally, parental and progeny isolates were phenotyped using a genetic stock of wheat genotypes representing 21 resistance genes. All S1 progeny isolates had virulence for 14 out of 15 loci where the parental isolate was virulent. This was consistent with the hypothesis that virulence in plant pathogens is often recessive to avirulence, i.e., only expressed in a homozygous state. Furthermore, no segregation was observed for five out of six loci, for which the parental isolate had an avirulent phenotype. The results for one of the two segregating virulence/avirulence loci suggested that the parental isolate was heterozygous with Avr alleles resulting in different but clearly avirulent phenotypes. The other locus indicated that additional genes modifying the phenotypic expression of avirulence were involved. PMID:25042987

Rodriguez-Algaba, Julian; Walter, Stephanie; Sørensen, Chris K; Hovmøller, Mogens S; Justesen, Annemarie F

2014-09-01

75

Berberis libanotica Ehrenb extract shows anti-neoplastic effects on prostate cancer stem/progenitor cells.  

PubMed

Cancer stem cells (CSCs), including those of advanced prostate cancer, are a suggested reason for tumor resistance toward conventional tumor therapy. Therefore, new therapeutic agents are urgently needed for targeting CSCs. Despite the minimal understanding of their modes of action, natural products and herbal therapies have been commonly used in the prevention and treatment of many cancers. Berberis libanotica Ehrenb (BLE) is a plant rich in alkaloids which may possess anti-cancer activity and a high potential for eliminating CSCs. We tested the effect of BLE on prostate cancer cells and our data indicated that this extract induced significant reduction in cell viability and inhibited the proliferation of human prostate cancer cell lines (DU145, PC3 and 22Rv1) in a dose- and time-dependent manner. BLE extract induced a perturbation of the cell cycle, leading to a G0-G1 arrest. Furthermore, we noted 50% cell death, characterized by the production of high levels of reactive oxidative species (ROS). Inhibition of cellular migration and invasion was also achieved upon treatment with BLE extract, suggesting a role in inhibiting metastasis. Interestingly, BLE extract had a major effect on CSCs. Cells were grown in a 3D sphere-formation assay to enrich for a population of cancer stem/progenitor cells. Our results showed a significant reduction in sphere formation ability. Three rounds of treatment with BLE extract were sufficient to eradicate the self-renewal ability of highly resistant CSCs. In conclusion, our results suggest a high therapeutic potential of BLE extract in targeting prostate cancer and its CSCs. PMID:25380390

El-Merahbi, Rabih; Liu, Yen-Nien; Eid, Assaad; Daoud, Georges; Hosry, Leina; Monzer, Alissar; Mouhieddine, Tarek H; Hamade, Aline; Najjar, Fadia; Abou-Kheir, Wassim

2014-01-01

76

Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats  

PubMed Central

Objective To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris (B. vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins extract on groups of rats rendered diabetic by injection of streptozotocin. Methods The phytochemical tests to detect the presence of different compounds were based on the visual observation of color change or formation of precipitate after the addition of specific reagents. Diabetes was induced in rats by intraperitoneal (i.p.) injection of streptozotocin (STZ) at a dose of 65 mg/kg bw. The fasting blood glucose levels were estimated by glucose oxidase-peroxidase reactive strips (Dextrostix, Bayer Diagnostics). Blood samples were taken by cutting the tip of the tail. Serum cholesterol and serum triglycerides were estimated by enzymatic DHBS colorimetric method. Results Administration of 62.5 and 25.0 mg/kg of saponins and aqueous extract respectively in normal rats group shows a significant hypoglycemic activity (32.33% and 40.17% respectively) during the first week. However, diabetic group treated with saponin extract produced a maximum fall of 73.1% and 76.03% at day 1 and day 21 compared to the diabetics control. Also, blood glucose levels of the diabetic rats treated with aqueous extract showed decrease of 78.79% on the first day and the effect remains roughly constant during 3 week. Both extracts also declined significantly biochemical parameters (20.77%-49.00%). The control in the loss of body weight was observed in treated diabetic rats as compared to diabetic controls. Conclusions These results demonstrated significant antidiabetic effects and showed that serum cholesterol and serum triglycerides levels were decreased, significantly, consequently this plant might be of value in diabetes treatment. PMID:23569815

Meliani, Nawel; Dib, Mohamed El Amine; Allali, Hocine; Tabti, Boufeldja

2011-01-01

77

Berberis libanotica Ehrenb Extract Shows Anti-Neoplastic Effects on Prostate Cancer Stem/Progenitor Cells  

PubMed Central

Cancer stem cells (CSCs), including those of advanced prostate cancer, are a suggested reason for tumor resistance toward conventional tumor therapy. Therefore, new therapeutic agents are urgently needed for targeting CSCs. Despite the minimal understanding of their modes of action, natural products and herbal therapies have been commonly used in the prevention and treatment of many cancers. Berberis libanotica Ehrenb (BLE) is a plant rich in alkaloids which may possess anti-cancer activity and a high potential for eliminating CSCs. We tested the effect of BLE on prostate cancer cells and our data indicated that this extract induced significant reduction in cell viability and inhibited the proliferation of human prostate cancer cell lines (DU145, PC3 and 22Rv1) in a dose- and time-dependent manner. BLE extract induced a perturbation of the cell cycle, leading to a G0-G1 arrest. Furthermore, we noted 50% cell death, characterized by the production of high levels of reactive oxidative species (ROS). Inhibition of cellular migration and invasion was also achieved upon treatment with BLE extract, suggesting a role in inhibiting metastasis. Interestingly, BLE extract had a major effect on CSCs. Cells were grown in a 3D sphere-formation assay to enrich for a population of cancer stem/progenitor cells. Our results showed a significant reduction in sphere formation ability. Three rounds of treatment with BLE extract were sufficient to eradicate the self-renewal ability of highly resistant CSCs. In conclusion, our results suggest a high therapeutic potential of BLE extract in targeting prostate cancer and its CSCs. PMID:25380390

Eid, Assaad; Daoud, Georges; Hosry, Leina; Monzer, Alissar; Mouhieddine, Tarek H.; Hamade, Aline; Najjar, Fadia; Abou-Kheir, Wassim

2014-01-01

78

Hepatoprotective Effects of Berberis vulgaris L. Extract/? Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice  

PubMed Central

The present study investigated the capacity of formulated Berberis vulgaris extract/?-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/?-cyclodextrin treatment prevents hepatic injury induced by CCl4 and can be considered for further nutraceutical studies. PMID:22942749

Hermenean, Anca; Popescu, Cristina; Ardelean, Aurel; Stan, Miruna; Hadaruga, Nicoleta; Mihali, Ciprian-Valentin; Costache, Marieta; Dinischiotu, Anca

2012-01-01

79

Hepatoprotective Effects of Berberis vulgaris L. Extract/? Cyclodextrin on Carbon Tetrachloride-Induced Acute Toxicity in Mice.  

PubMed

The present study investigated the capacity of formulated Berberis vulgaris extract/?-cyclodextrin to protect liver against CCl(4)-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl(4) on the 8th day. After 24 h of CCl(4) administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl(4) was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/?-cyclodextrin treatment prevents hepatic injury induced by CCl(4) and can be considered for further nutraceutical studies. PMID:22942749

Hermenean, Anca; Popescu, Cristina; Ardelean, Aurel; Stan, Miruna; Hadaruga, Nicoleta; Mihali, Ciprian-Valentin; Costache, Marieta; Dinischiotu, Anca

2012-01-01

80

Legitimate seed dispersal by lizards in an alpine habitat: The case of Berberis empetrifolia (Berberidaceae) dispersed by Liolaemus belii (Tropiduridae)  

NASA Astrophysics Data System (ADS)

In this study we determined the effect of seed passage through Liolaemus bellii lizard digestive tracts on germination of fleshy-fruited Andean shrub species Berberis empetrifolia (Berberidaceae), and evaluated the effect of this passage on seed coat characteristics. In addition, we assessed the spatial patterns of fecal deposition by lizards onto various microhabitats available in the Andean environments of central Chile. The germination rate and the final percentage of lizard-ingested B. empetrifolia seeds was greater than control seeds. Comparing photographs and seed coat histological cuts, we suggest that the cutine wax present on seed coats from lizard-ingested seeds was probably removed by abrasion inside the lizards' digestive tract. Sixty-two percent of the lizard's feces was deposited on bare soil near rocks commonly inhabited by lizards. However, this microhabitat represents only 29% of the available ground cover at the study site. By enhancing seed germination and depositing seeds onto potential safe sites for recruitment, the lizard Liolaemus bellii is acting, at least qualitatively, as an effective disperser of Berberis empetrifolia.

Celedón-Neghme, Constanza; San Martin, Leonardo A.; Victoriano, Pedro F.; Cavieres, Lohengrin A.

2008-05-01

81

Antihyperglycemic and Antihyperlipidemic Effects of Fruit Aqueous Extract of Berberis integerrima Bge. in Streptozotocin-induced Diabetic Rats.  

PubMed

Use of medicinal plants for attenuation of hyperglycemia and restoration of lipids disorder to normal level is clinically very important. The aim of present study was to evaluate the effects of Berberis integerrima Bge. fruit aqueous extract (BIFAE) on blood glucose and lipid profile in streptozotocin (STZ) - induced diabetic rats. The STZ-induced diabetic rats were treated by fruit aqueous extract of Berberis integerrima Bge. at doses (250 and 500 mg/Kg bw) and glibenclamide (0.6 mg/Kg bw) for 42 days by gavage. Blood glucose levels and body weights of rats were measured on weeks 0, 2, 4 and 6. Total lipid levels were determined in normal and STZ-induced diabetic rats after administration of the BIFAE and glibenclamide for 42 days. STZ-induced diabetic rats showed a significant (P<0.001) increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C) while body weight and high density lipoprotein HDL-cholesterolan (HDL-C) were significantly(P<0.001) decreased compared to normal rats. Daily administration of BIFAE did not possess the hypoglycemic and hypolipidaemic activity in STZ- diabetic rats during 6-week treatment period. Results indicate the usage of BIFAE in traditional medicine for the treatment of diabetes may need more investigation. PMID:25587320

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid

2014-01-01

82

Antihyperglycemic and Antihyperlipidemic Effects of Fruit Aqueous Extract of Berberis integerrima Bge. in Streptozotocin-induced Diabetic Rats  

PubMed Central

Use of medicinal plants for attenuation of hyperglycemia and restoration of lipids disorder to normal level is clinically very important. The aim of present study was to evaluate the effects of Berberis integerrima Bge. fruit aqueous extract (BIFAE) on blood glucose and lipid profile in streptozotocin (STZ) - induced diabetic rats. The STZ-induced diabetic rats were treated by fruit aqueous extract of Berberis integerrima Bge. at doses (250 and 500 mg/Kg bw) and glibenclamide (0.6 mg/Kg bw) for 42 days by gavage. Blood glucose levels and body weights of rats were measured on weeks 0, 2, 4 and 6. Total lipid levels were determined in normal and STZ-induced diabetic rats after administration of the BIFAE and glibenclamide for 42 days. STZ-induced diabetic rats showed a significant (P<0.001) increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C) while body weight and high density lipoprotein HDL-cholesterolan (HDL-C) were significantly(P<0.001) decreased compared to normal rats. Daily administration of BIFAE did not possess the hypoglycemic and hypolipidaemic activity in STZ- diabetic rats during 6-week treatment period. Results indicate the usage of BIFAE in traditional medicine for the treatment of diabetes may need more investigation. PMID:25587320

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid

2014-01-01

83

Century-old mystery of Puccinia striiformis life history solved with the identification of Berberis as an alternate host.  

PubMed

The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P. striiformis. Analyses using real-time polymerase chain reaction and DNA sequence confirmed the rust fungus as P. striiformis. Pycnia and aecia were produced on B. chinensis, B. holstii, B. koreana, and B. vulgaris after inoculation using germinating telia of P. striiformis f. sp. tritici. Wheat inoculated with aeciospores from B. chinensis resulted in uredinia, which demonstrated that Berberis spp. also serve as alternate hosts for the wheat stripe rust pathogen. The elucidation of the complete life history for P. striiformis f. sp. tritici will provide a powerful tool to rapidly advance our knowledge of the genetics of this rust fungus, and will lead to the development of improved strategies for a better control of stripe rust. PMID:20373963

Jin, Yue; Szabo, Les J; Carson, Martin

2010-05-01

84

The antihypertensive and vasodilator effects of aqueous extract from Berberis vulgaris fruit on hypertensive rats.  

PubMed

The aqueous extract from Berberis vulgaris fruit (B.V.) was tested to evaluate its antihypertensive effects on DOCA-induced hypertension in the rats. Hypertension was induced in male Sprague-Dawley rats (200-250 g) by DOCA-salt injection (20 mg/kg, twice weekly, for 5 weeks, s.c.) plus NaCl (1%) which was added to the animals' drinking water. Then 5 weeks later, the rats were anaesthetized with thiopental (30 mg/kg, i.p.) and the arterial blood pressure was measured. The mean arterial blood pressure and heart rate were 231 +/- 6.4 (mmHg) and 506 +/- 12 (beats/min), respectively. Administration of B.V. extracts significantly reduced the rat arterial blood pressure. In in vitro studies, rings of descending aorta were cut and mounted for isometric tension recording in an organ chamber containing Krebs solution. Mesenteric beds were also removed and perfused with Krebs solution. After 1 h of stabilization, preparations (aortic rings or mesenteric beds) were precontracted with phenylephrine (10(-5) M), then different concentrations of B.V. (0.4, 2 and 4 mg/mL) were added which caused a relaxation in these vessels. To investigate the mechanism of action of the extract, the tissues were incubated with either L-NAME (10(-5) M) or indomethacin (10(-5) M) for 20 min. In the aortic rings L-NAME pretreatment could only reduce the vasodilatory effects of a low concentration of B.V. (0.4 mg/mL), but indomethacin was without effect. In isolated perfused mesenteric beds preincubation with either L-NAME or indomethacin did not modify the vasodilator effects of the aqueous extract from B.V. fruit. The present results suggest that the antihypertensive and vasodilatory effects of B.V. fruit extract are mainly endothelial-independent and it may be used to treat hypertension, a status with endothelial dysfunction. PMID:15934023

Fatehi-Hassanabad, Zahra; Jafarzadeh, Mostafa; Tarhini, Ahmad; Fatehi, Mohammad

2005-03-01

85

DCEG Fellows Lam and Loftfield Successfully Defend Dissertations  

Cancer.gov

Clara Lam, Ph.D., successfully defended her doctoral dissertation at the George Washington School of Public Health. Her dissertation, titled “Identifying risk factors for second malignancies after non-Hodgkin lymphoma,” represented the first analytic study of second cancers after NHL to consider both treatments and immune-related medical conditions as potential second cancer risk factors.

86

Seedling Populations Produced by Colored-leaf Genotypes of Japanese Barberry (Berberis thunbergii DC.) Contain Seedlings with Green Leaf Phenotype1  

Microsoft Academic Search

The leaf color of seedling populations derived from ornamental genotypes of Japanese barberry ( Berberis thunbergii DC.) was evaluated to determine whether nursery selections of this important landscape plant could be expected to produce green-leaf progeny or seedlings with leaf color resembling the purple-leaf or yellow-leaf parent. This is a compelling inquiry since nearly all B. thunbergii plants found within

Jonathan M. Lehrer; Mark H. Brand; Jessica D. Lubell

2006-01-01

87

The role of the native soil community in the invasion ecology of spotted ( Centaurea maculosa auct. non Lam.) and diffuse ( Centaurea diffusa Lam.) knapweed  

Microsoft Academic Search

Diffuse (Centaurea diffusa Lam.) and spotted knapweed (Centaurea maculosa auct. non Lam.) are damaging exotic plant invaders of North American rangelands. Although existing weed management techniques can successfully reduce negative impacts of knapweeds, these plants continue to spread in an unpredictable manner. The successful spread of knapweeds into native plant communities may be driven, in part, by interactions between these

Paul J. Meiman; Edward F. Redente; Mark W. Paschke

2006-01-01

88

TSC2 epigenetic defect in primary LAM cells. Evidence of an anchorage-independent survival  

PubMed Central

Tuberous sclerosis complex (TSC) is caused by mutations in TSC1 or TSC2 genes. Lymphangioleiomyomatosis (LAM) can be sporadic or associated with TSC and is characterized by widespread pulmonary proliferation of abnormal ?-smooth muscle (ASM)-like cells. We investigated the features of ASM cells isolated from chylous thorax of a patient affected by LAM associated with TSC, named LAM/TSC cells, bearing a germline TSC2 mutation and an epigenetic defect causing the absence of tuberin. Proliferation of LAM/TSC cells is epidermal growth factor (EGF)-dependent and blockade of EGF receptor causes cell death as we previously showed in cells lacking tuberin. LAM/TSC cells spontaneously detach probably for the inactivation of the focal adhesion kinase (FAK)/Akt/mTOR pathway and display the ability to survive independently from adhesion. Non-adherent LAM/TSC cells show an extremely low proliferation rate consistent with tumour stem-cell characteristics. Moreover, LAM/TSC cells bear characteristics of stemness and secrete high amount of interleukin (IL)-6 and IL-8. Anti-EGF receptor antibodies and rapamycin affect proliferation and viability of non-adherent cells. In conclusion, the understanding of LAM/TSC cell features is important in the assessment of cell invasiveness in LAM and TSC and should provide a useful model to test therapeutic approaches aimed at controlling their migratory ability. PMID:24606538

Lesma, Elena; Ancona, Silvia; Sirchia, Silvia M; Orpianesi, Emanuela; Grande, Vera; Colapietro, Patrizia; Chiaramonte, Eloisa; Di Giulio, Anna Maria; Gorio, Alfredo

2014-01-01

89

Stiochiometry of maltodextrin-binding sites in LamB, an outer membrane protein from Escherichia coli  

SciTech Connect

The authors have directly measured the stoichiometry of maltodextrin-binding sites in LamB. Scatchard plots and computer fitting of flow dialysis (rate-of-dialysis) experiments clearly establish three independent binding sites per LamB trimer, with a dissociation constant of approximately 60 {mu}M for maltoheptaose. The current model for LamB's function as a specific pore is discussed with respect to the symmetry in LamB's kinetic properties and the implications of their results.

Gehring, K.; Cheng, Chaohsiung; Jap, B.K. (Lawrence Berkeley Lab., CA (United States)); Nikaido, H. (Univ. of California, Berkeley (United States))

1991-03-01

90

Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids.  

PubMed

Extracts obtained from the roots of Berberidaceae species have been used in Eastern and Bulgarian folk medicine in rheumatic and other chronic inflammatory disorders. The investigations of the chemical composition and immunological properties show that their activity is mainly due to the alkaloid constituents. In the present study the anti-inflammatory properties of total ethanol extract (TEE), three alkaloid fractions, a major alkaloid berberine and oxyacanthine isolated from Berberis vulgaris roots were compared. All these were applied in acute inflammation (carrageenan- and zymosan-induced paw oedema), as the TEE showed the highest reducing effect. Their ability to alter in vivo and in vitro complement activity was determined. Also, the TEE was most effective in a chronic inflammatory model of adjuvant arthritis. The protoberberine fractions Bv2, Bv3 and berberine suppressed a delayed type hypersensitivity (DTH) reaction. Fraction Bv1 and berberine diminished antibody response against SRBC in vivo. The in vitro treatment of splenocytes with berberine showed that the anti-SRBC antibody synthesis was influenced in a different manner depending on the time course of its application. Oxyacanthine was less effective than berberine in the tests used. PMID:9080249

Ivanovska, N; Philipov, S

1996-10-01

91

Comparative Study of Berberis vulgaris Fruit Extract and Berberine Chloride Effects on Acetic Acid-Induced Colitis in Rats.  

PubMed

Antioxidant and immunomodulatory effects of anthocyanins are abundant in berberry fruits suggesting that they may have beneficial effects on inflammatory bowel diseases (IBD). The present study was carried out to investigate the anti-colitic effect of Berberis vulgaris fruit extract (BFE) compared to berberine chloride (BEC) and corticosteroids using an animal model of acetic acid induced experimental colitis. BFE with three different doses (375, 750, and 1500 mg/Kg) was administered orally or rectally prior to ulcer induction. BEC (10 mg/Kg), prednisolone (5 mg/Kg), hydrocortisone acetate enema (20 mg/Kg) and normal saline (5 mL/Kg) were considered as respective controls. The tissue was assessed macroscopically for damage scores, area, index and weight/length ratio. They were also examined histopathologically for inflammation extent and severity, crypt damage, invasion involvement and total colitis index. Results indicated that greater doses of oral BFE (750, 1500 mg/Kg) as well as BEC (10 mg/Kg) were effective to protect against colonic damage. By rectal pretreatment, the extract was only effective to diminish the ulcer index and the efficacy was not significant for mucosal inflammation parameters. In conclusion BFE, which is nearly devoid of berberine, was effective to protect against colitis and this might be attributed to its anthocyanin constituents. PMID:24363687

Minaiyan, Mohsen; Ghannadi, Alireza; Mahzouni, Parvin; Jaffari-Shirazi, Elham

2011-01-01

92

Comparative Study of Berberis vulgaris Fruit Extract and Berberine Chloride Effects on Acetic Acid-Induced Colitis in Rats  

PubMed Central

Antioxidant and immunomodulatory effects of anthocyanins are abundant in berberry fruits suggesting that they may have beneficial effects on inflammatory bowel diseases (IBD). The present study was carried out to investigate the anti-colitic effect of Berberis vulgaris fruit extract (BFE) compared to berberine chloride (BEC) and corticosteroids using an animal model of acetic acid induced experimental colitis. BFE with three different doses (375, 750, and 1500 mg/Kg) was administered orally or rectally prior to ulcer induction. BEC (10 mg/Kg), prednisolone (5 mg/Kg), hydrocortisone acetate enema (20 mg/Kg) and normal saline (5 mL/Kg) were considered as respective controls. The tissue was assessed macroscopically for damage scores, area, index and weight/length ratio. They were also examined histopathologically for inflammation extent and severity, crypt damage, invasion involvement and total colitis index. Results indicated that greater doses of oral BFE (750, 1500 mg/Kg) as well as BEC (10 mg/Kg) were effective to protect against colonic damage. By rectal pretreatment, the extract was only effective to diminish the ulcer index and the efficacy was not significant for mucosal inflammation parameters. In conclusion BFE, which is nearly devoid of berberine, was effective to protect against colitis and this might be attributed to its anthocyanin constituents. PMID:24363687

Minaiyan, Mohsen; Ghannadi, Alireza; Mahzouni, Parvin; Jaffari-Shirazi, Elham

2011-01-01

93

Effect of root bark extract of Berberis vulgaris L. on Leishmania major on BALB/c mice.  

PubMed

Leishmaniasis is one of the most important diseases transmitted by arthropod. This disease is present in 88 countries. Approximately 400 million people are at risk, and 12 million are involved. We aimed to examine the application of ethanolic extract of the root bark of Berberis vulgaris L. for treatment of mice infected with cutaneous leishmaniasis. At first, 40 BALB/c mice were infected to Leishmania major promastigotes and were divided in two groups A and B. Then, each of A and B groups were divided to two subgroups. Mice from subgroup A1 were treated with 10% root bark alcoholic extract, and mice from subgroup A2 were treated with only alcohol (control). Mice from subgroup B1 were treated with 20% root bark alcoholic extract, and mice from subgroup B2 were treated with only alcohol (control). The 90% recovery was found in the mice treated with 20% root bark extract, and 55% recovery was found with 10% root bark extract, but in the control group, 0% recovery was found. The results of our study showed that the lotion of root bark extract has good suppression effects on parasites. Therefore, it might be a pro for developing new antileishmanial drugs. PMID:24337510

Salehabadi, Alireza; Karamian, Mahdi; Farzad, Motevalli Haghi; Namaei, Mohammad Hasan

2014-03-01

94

Antioxidant Properties of Berberis aetnensis C. Presl (Berberidaceae) Roots Extract and Protective Effects on Astroglial Cell Cultures  

PubMed Central

Berberis aetnensis C. Presl (Berberidaceae) is a bushy-spiny shrub common on Mount Etna (Sicily). We demonstrated that the alkaloid extract of roots of B. aetnensis C. Presl contains prevalently berberine and berbamine, possesses antimicrobial properties, and was able to counteract the upregulation evoked by glutamate of tissue transglutaminase in primary rat astroglial cell cultures. Until now, there are no reports regarding antioxidant properties of B. aetnensis C. Presl collected in Sicily. Air-dried, powdered roots of B. aetnensis C. Presl were extracted, identified, and quantified by HPLC. We assessed in cellular free system its effect on superoxide anion, radicals scavenging activity of antioxidants against free radicals like the 1,1-diphenyl-2-picrylhydrazyl radical, and the inhibition of xanthine oxidase activity. In primary rat astroglial cell cultures, exposed to glutamate, we evaluated the effect of the extract on glutathione levels and on intracellular production of reactive oxygen species generated by glutamate. The alkaloid extract of B. aetnensis C. Presl inhibited superoxide anion, restored to control values, the decrease of GSH levels, and the production of reactive oxygen species. Potent antioxidant activities of the alkaloid extract of roots of B. aetnensis C. Presl may be one of the mechanisms by which the extract is effective against health disorders associated to oxidative stress. PMID:25177720

Campisi, Agata; Bonfanti, Roberta; Raciti, Giuseppina; Amodeo, Andrea; Mastrojeni, Silvana; Ragusa, Salvatore; Iauk, Liliana

2014-01-01

95

Time domain modelling of room acoustics Y.W. Lam and J. Hargreaves  

E-print Network

Time domain modelling of room acoustics Y.W. Lam and J. Hargreaves University of Salford, School of Computing, Science and Engineering, M5 4WT Salford, UK y.w.lam@salford.ac.uk Proceedings of the Acoustics advantages for room acoustics investigations. It directly generates the efficient for broadband calculations

Paris-Sud XI, Université de

96

Hydrocarbon composition of crude oil from Lam Bank  

SciTech Connect

The authors discuss the crude oil from a new offshore field called the Lam Bank in the Caspian Sea. A segregated commercial crude was distilled and the distillation data is shown. In order to determine the content of n-paraffins, the naphthenic-paraffinic part of the narrow cuts was subjected to adsorptive separation on CaA zeolite. Owing to the high contents of naphthenic and isoparaffinic hydrocarbons and the low content of aromatic hydrocarbons in the distillate part, this crude can be used to produce high-quality fuels and oils by the use of the dewaxing processes.

Samedova, F.I.; Agaeva, R.M.; Alieva, F.Z.; Valiev, M.A.

1987-07-01

97

Study of Load Alleviation and Mode Suppression (LAMS) on the YF-12A airplane  

NASA Technical Reports Server (NTRS)

The potentials and capability for implementing a LAMS (load alleviation and mode suppression) system on the YF-12A for the purpose of flight research were evaluated. The nature of the research is to minimize the design risk in application of LAMS to future aircraft. The results of the study show that the YF-12A would be a suitable test bed for continuing development of LAMS technology. This was demonstrated by defining five candidate LAMS systems and analytically evaluating them with regard to performance and mechanization. Each of the five systems used a different combination of force producers. A small canard vane or a mass-reaction device mounted near the cockpit was considered as a possible LAMS force producer, together with the existing inboard and outboard elevons. It was concluded that a combination of canard vane and outboard elevons would provide the most effective system for the YF-12A.

Edinger, L. D.; Chenk, F. L.; Curtis, A. R.

1972-01-01

98

Removal of nitrobenzene from aqueous solution by a novel lipoid adsorption material (LAM).  

PubMed

In this study, a novel adsorbent referred to as a lipoid adsorption material (LAM) was synthesized with a hydrophobic nucleolus (triolein) and hydrophilic membrane structure (polyamide). The LAM was applied to the adsorption and removal of nitrobenzene from aqueous systems. Experiments were carried out to investigate the adsorption behavior of nitrobenzene on LAM, including the development of adsorption isotherms, the determination of adsorption kinetics, and to explore the influence of adsorbent dosage, contact time, temperature and the initial concentration of nitrobenzene on adsorption. The performance of LAM was compared with equal amounts of granular activated carbon (GAC) for adsorption. The adsorption isotherms for LAM were found to be described by the Linear equation, while the adsorption isotherms for granular activated carbon (GAC) were described by the Freundlich equation. Results indicated that the adsorption of nitrobenzene by LAM occurred mainly due to the partition function caused by the triolein nucleolus. Two kinetics models, pseudo-first-order and pseudo-second-order models were used to fit the experimental data for LAM adsorption. By comparing the correlation coefficients, it was found that the pseudo-first-order model was most suitable to describe the adsorption of nitrobenzene on LAM. The results also indicated that the factors that affect the adsorption rate would be either the nitrobenzene concentration or the character of the adsorbent. Thermodynamic calculations indicated that the adsorption of nitrobenzene on LAM was spontaneous and was an exothermic reaction. With an initial nitrobenzene concentration of 200 ?g l(-1), an equilibrium concentration was reached within 8h using LAM as an adsorbent and the average removal efficiency was 94.3%. For GAC, the adsorption equilibrium was achieved after 12h with a 91.9% nitrobenzene removal efficiency. PMID:22277343

Wen, Qinxue; Chen, Zhiqiang; Lian, Jiaxiang; Feng, Yujie; Ren, Nanqi

2012-03-30

99

Anatomical investigation of flower of Butea monosperma Lam.  

PubMed Central

Background: Butea frondosa Roxb. and Koen. syn. Butea monosperma Lam. (Leguminosae or Fabaceae) is a tree grows up to the height of 8 m at the age 50 years. Its flowers are being used in traditional medicine for the treatment of ulcer, inflammation, hepatic disorder, and eye diseases. Aims: The present study was aimed at establishing the microscopic characteristics of flower B. monosperma Lam. Materials and Methods: Histological evaluation of flowers was done using standard procedures. Images of microscopic characters were taken at different magnifications using Nikon Labphoto 2 microscopic Unit. Perkin Elmer 5000 an atomic absorption spectrophotometer was employed for elemental analysis. Results: In the study, microscopic characters of floral parts were investigated in transverse section and the flower powder. The current study reveals the presence of pollen grains, ovary (OV), and trichomes in their flower powder. Different cell components were studied, and their sizes were measured. Elemental analysis showed the presence of Zn 52.2 ?g/g and Cu 36.3 ?g/g were major contents, whereas Cr, Mn, and Pd were minor contents in dried flower powder. Conclusion: The current study paves the way to provide standard information related to the presence of essential elements in the flower. Microscopic characters of the flower and its quantitative measurement of cell components will help to identify the plant and also help to improvise the existing monograph of B. monosperma in the Ayurvedic pharmacopoeia.

Muthuswamy, Ragunathan; Senthamarai, R.

2014-01-01

100

Berberis vulgaris root extract alleviates the adverse effects of heat stress via modulating hepatic nuclear transcription factors in quails.  

PubMed

To evaluate the action mode of Berberis vulgaris root extract in the alleviation of oxidative stress, female Japanese quails (n 180, aged 5 weeks) were reared, either at 22°C for 24 h/d (thermoneutral, TN) or 34°C for 8 h/d (heat stress, HS), and fed one of three diets: diets containing 0, 100 or 200 mg of B. vulgaris root extract per kg for 12 weeks. Exposure to HS depressed feed intake by 8·5% and egg production by 12·1%, increased hepatic malondialdehyde (MDA) level by 98·0% and decreased hepatic superoxide dismutase, catalase and glutathione peroxidase activities by 23·5, 35·4 and 55·7%, respectively (P<0·001 for all). There were also aggravations in expressions of hepatic NF-?B and heat-shock protein 70 (HSP70) by 42 and 43%, respectively and suppressions in expressions of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and haeme-oxygenase 1 (HO-1) by 57 and 61%, respectively, in heat-stressed quails (P<0·001 for all). As supplemental B. vulgaris extract increased, there were linear increases in performance parameters, activities of antioxidant enzymes and hepatic Nrf2 and HO-1 expressions (P<0·001 for all) and linear decreases in hepatic MDA level and NF-?B and HSP70 expressions at a greater extent in quails reared under TN condition and those reared under HS condition. In conclusion, dietary supplementation of B. vulgaris root extract to quails reduces the detrimental effects of oxidative stress and lipid peroxidation resulting from HS via activating the host defence system at the cellular level. PMID:23312115

Sahin, Kazim; Orhan, Cemal; Tuzcu, Mehmet; Borawska, Maria H; Jab?onski, Jakub; Guler, Osman; Sahin, Nurhan; Hayirli, Armagan

2013-08-01

101

Analysis of Lymphangioleiomyomatosis (LAM) patent tomograms: Stereology and modeling  

SciTech Connect

Lymphangioleiomyomatosis (LAM) is an insidious disease of young women which attacks the lungs and if untreated results in death. Treatment is radical. Currently a woman diagnosed as having LAM (usually by computed tomography (CT) of the lung) is run through an exhausting set of tests and exercises to determine the extent of the disease. Image processing techniques have been able to quantify the tomograms by providing a histogram of the number of cyst intercepts of a given area. The images are digital, so the data are discrete, and the areas are in terms of square pixels. It is hoped that by analyzing the data and by recovering the probability density of the cysts themselves one can provide a set of parameters that will aid in the early diagnosis of the disease and that will correlate well with the physiology. The desire to invert the data, that is to determine the probability density of the cysts from the probability density of the circular intersections of the cysts with the tomographic plane, led to a re-examination of the Wicksell equation. In Section 2, the authors transform Wicksell`s equation in diameter (a generalized Abel equation) into an equation in areas (the original Abel equation). In Section 3 they present the adjoint equation in several equivalent forms. Analytic solutions of the adjoint equation necessary for later use are developed in Section 4. Outside of the framework of integral equations they have done some modeling of the data. They find that, to a very high degree of reliability, the data follows a simple power law. The distribution of spheres likewise follows a simple power law. These considerations are given in Section 5. Then in Section 6 they explore the exponential model as an alternative. Section 7 solves the Wicksell equation and uses the Golberg device, via the adjoint, to solve a related problem. They finally get to the number of cysts and their average size in Section 8. Their conclusions are in Section 9.

Faber, V.; Wing, G.M.; Winter, L.; Zahrt, J.D. [Los Alamos National Lab., NM (United States). Computing, Information, and Communications Div.

1995-02-01

102

[Identification of a confused species of Euphorbia hirta L. E. indica Lam].  

PubMed

The article reported the morphological and histological identification for Euporbia hirta L. and its confused species E. indica Lam.. It provided evidences for identifying Euphorbia hirta L.. PMID:11341025

Chu, X; Cao, L; Yuan, C

2001-01-01

103

Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats  

Microsoft Academic Search

This study was carried out to evaluate the effect of Moringa oleifera Lam (Moringa) seed extract on liver fibrosis. Liver fibrosis was induced by the oral administration of 20% carbon tetrachloride (CCl4), twice weekly and for 8weeks. Simultaneously, M.oleifera Lam seed extract (1g\\/kg) was orally administered daily. The biochemical and histological results showed that Moringa reduced liver damage as well

Alaaeldin A. Hamza

2010-01-01

104

LAM-1 and FAT Genes Control Development of the Leaf Blade in Nicotiana sylvestris.  

PubMed Central

Leaf primordia of the lam-1 mutant of Nicotiana sylvestris grow normally in length but remain bladeless throughout development. The blade initiation site is established at the normal time and position in lam-1 primordia. Anticlinal divisions proceed normally in the outer L1 and L2 layers, but the inner L3 cells fail to establish the periclinal divisions that normally generate the middle mesophyll core. The lam-1 mutation also blocks formation of blade mesophyll from distal L2 cells. This suggests that LAM-1 controls a common step in initiation of blade tissue from the L2 and L3 lineage of the primordium. Another recessive mutation (fat) was isolated in N. sylvestris that induces abnormal periclinal divisions in the mesophyll during blade initiation and expansion. This generates a blade approximately twice its normal thickness by doubling the number of mesophyll cell layers from four to approximately eight. Presumably, the fat mutation defines a negative regulator involved in repression of periclinal divisions in the blade. The lam-1 fat double mutant shows radial proliferation of mesophyll cells at the blade initiation site. This produces a highly disorganized, club-shaped blade that appears to represent an additive effect of the lam-1 and fat mutations on blade founder cells. PMID:12271096

McHale, NA

1993-01-01

105

In vitro biological assessment of berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects  

PubMed Central

Background Berberis vulgaris is a well known plant with traditional herbal medical history. The aims of this study was to bioscreen and compare the in vitro biological activity (antioxidant, cholinergic, antidaibetic and the anticancer) of barberry crude extract and berberine active compound. Methods The effect of B. vulgaris extract and berberine chloride on cellular thiobarbituric acid reactive species (TBARS) formation, diphenyle–?-picrylhydrazyl (DPPH) oxidation, cellular nitric oxide (NO) radical scavenging capability, superoxide dismutase (SOD), glutathione peroxidase (GPx), acetylcholinesterase (AChE) and ?-gulcosidase activities were spectrophotometrically determined. On the other hand, the effect of extract and berberine as anticancer was estimated on three different cell lines which were MCF-7, HepG-2, and Caco-2 cells by using neutral red uptake assay which compared with control normal cells (PBMC). Results Our results showed that barberry crude extract contains 0.6 mg berberine/mg crude extract. Barberry extract showed potent antioxidative capacity through decreasing TBARS, NO and the oxidation of DPPH that associated with GPx and SOD hyperactivation. Inhibitory effect of berberis crude extract on ?-glucosidase was more potent than that of berberine chloride, while both had the same AChE inhibitory effect. Besides, different concentrations of both berberine chloride and barberry ethanolic extract showed to have no growth inhibitory effect on normal blood cells (PBMC). Otherwise, both berberine chloride and barberry ethanolic extract showed to have inhibitory effect on the growth of breast, liver and colon cancer cell lines (MCF7, HepG2 and CACO-2, respectively) at different incubation times starting from 24 hrs up to 72 hrs and the inhibitory effect increased with time in a dose dependant manner. Conclusion This work demonstrates the potential of the barberry crude extract and its active alkaloid, berberine, on suppressing lipid peroxidation, suggesting a promising use in the treatment of hepatic oxidative stress, Alzheimer and idiopathic male factor infertility. Beside, berberis vulgaris ethanolic extract is safe non-toxic extract as it was not inhibit the growth of PBMC that can induce cancer cell death that could return to its powerful antioxidant activity. PMID:24007270

2013-01-01

106

Benzylcarbamothioethionate from root bark of Moringa oleifera Lam. and its toxicological evaluation (Bencilcarbamotioetionato de la raíz de Moringa oleifera Lam. y su evaluación toxicológica)  

Microsoft Academic Search

A new compound, benzylcarbamothioethionate (1), was isolated from the chloroform soluble fraction of the ethanolic extract of the root bark of Moringa oleifera Lam. Its structure was established on the basis of 1D and 2D-NMR and mass spectroscopy. The acute toxicity studies of chloroform extract and compound 1 were performed on Long Evan's rats using four groups (two controls and

Farjana NIKKON; Sohel HASAN; Kazi Abdus SALAM; Mohammed A. MOSADDIK; Proma KHONDKAR; Mohammed Ekramul HAQUE; Mukhlesur RAHMAN

107

New phytoconstituents from the aerial parts of Fumaria parviflora Lam  

PubMed Central

Fumaria parviflora Lam. (Fumariaceae) is an annual herb found throughout the world. Traditionally it has great significance in various disorders. In folk medicine of Turkey it is used against hepato-biliary dysfunction and imported from Iran. In Charaka and Sushruta, it is recommended for treatment of fevers, blood disorders, chronic skin diseases, urinary diseases and cough. The compounds were isolated from methanolic extract of the plants by column chromatography using silica gel (60-120 mesh) as stationary phase and structure of the isolated compounds have been established on the basis of spectral data analysis and chemical reactions. Phytochemical investigation of its aerial parts led to the isolation of five new compounds characterized as (5?H,11?H)-8-oxo-homoiridolide (1), n-docosanyl-2-O-?-D-glucopyranosyl salicylate (2), 2-methyl-6-hydroxymethylenedodecan-10-oyl-12, 15-olide14-O-?-D-xylopyranoside (3), 4-oxo-stigmast-5-en-3?-ol-D-glucopyranoside (4) and salicylic acid-O-?-D-xylopyranoside (5) along with the known compounds ?-D-glucopyranosyl hexadecanoate (6) and ?-D-glucopyranosyl- (2 ? 1?)-?-D-glucopyranoside (7). The isolated compounds are useful as they will provide essential data and information for the further researchers and development of effective analytical marker for identity, purity and quality control of this traditional plant in future. PMID:24959414

Jameel, Mohammad; Ali, Abuzer; Ali, Mohammed

2014-01-01

108

New phytoconstituents from the aerial parts of Fumaria parviflora Lam.  

PubMed

Fumaria parviflora Lam. (Fumariaceae) is an annual herb found throughout the world. Traditionally it has great significance in various disorders. In folk medicine of Turkey it is used against hepato-biliary dysfunction and imported from Iran. In Charaka and Sushruta, it is recommended for treatment of fevers, blood disorders, chronic skin diseases, urinary diseases and cough. The compounds were isolated from methanolic extract of the plants by column chromatography using silica gel (60-120 mesh) as stationary phase and structure of the isolated compounds have been established on the basis of spectral data analysis and chemical reactions. Phytochemical investigation of its aerial parts led to the isolation of five new compounds characterized as (5?H,11?H)-8-oxo-homoiridolide (1), n-docosanyl-2-O-?-D-glucopyranosyl salicylate (2), 2-methyl-6-hydroxymethylenedodecan-10-oyl-12, 15-olide14-O-?-D-xylopyranoside (3), 4-oxo-stigmast-5-en-3?-ol-D-glucopyranoside (4) and salicylic acid-O-?-D-xylopyranoside (5) along with the known compounds ?-D-glucopyranosyl hexadecanoate (6) and ?-D-glucopyranosyl- (2 ? 1')-?-D-glucopyranoside (7). The isolated compounds are useful as they will provide essential data and information for the further researchers and development of effective analytical marker for identity, purity and quality control of this traditional plant in future. PMID:24959414

Jameel, Mohammad; Ali, Abuzer; Ali, Mohammed

2014-04-01

109

New steroidal glycosides from Hosta plantaginea (Lam.) Aschers.  

PubMed

Four new furostanol glycosides were isolated from the flowers of Hosta plantaginea (Lam.) Aschers. On the basis of spectroscopic methods including 1D and 2D NMR experiments, their structures were elucidated as 26-O-?-d-glucopyranosyl-(25R)-22-O-methyl-5?-furostan-2?,3?,22?,26-tetrol 3-O-?-l-rhamnopyranosyl-(1 ? 4)-O-?-d-xylopyranosyl-(1 ? 3)-[O-?-d-glucopyranosyl-(1 ? 2)]-O-?-d-glucopyranosyl-(1 ? 4)-?-d-galactopyranoside (hostaplantagineoside A, 1), 26-O-?-d-glucopyranosyl-(25R)-5?-furostan-20(22)-ene-2?,3?,26-triol-3-O-?-d-glucopyranosyl-(1 ? 2)-[O-?-d-xylopyranosyl-(1 ? 3)]-O-?-d-glucopyranosyl-(1 ? 4)-?-d-galactopyranoside (hostaplantagineoside B, 2), 26-O-?-d-glucopyranosyl-(25R)-5?-furostan-22(23)-ene-2?,3?,20?,26-tetraol-3-O-?-d-glucopyranosyl-(1 ? 2)-[O-?-d-xylopyranosyl-(1 ? 3)]-O-?-d-glucopyranosyl-(1 ? 4)-O-?-d-galactopyranoside (hostaplantagineoside C, 3), 26-O-?-d-glucopyranosyl-(25R)-5?-furostan-20(22)-ene-2?,3?,26-triol-3-O-?-l-rhamnopyranosyl-(1 ? 4)-O-?-d-xylopyranosyl-(1 ? 3)-[O-?-d-glucopyranosyl-(1 ? 2)]-O-?-d-glucopyranosyl-(1 ? 4)-?-d-galactopyranoside (hostaplantagineoside D, 4). PMID:25559690

Li, Xiao-Juan; Wang, Li; Xue, Pei-Feng; Xie, Hong-Xia; Wei, Hui; Wang, Jing

2015-03-01

110

Estradiol and mTORC2 cooperate to enhance prostaglandin biosynthesis and tumorigenesis in TSC2-deficient LAM cells.  

PubMed

Lymphangioleiomyomatosis (LAM) is a progressive neoplastic disorder that leads to lung destruction and respiratory failure primarily in women. LAM is typically caused by tuberous sclerosis complex 2 (TSC2) mutations resulting in mTORC1 activation in proliferative smooth muscle-like cells in the lung. The female predominance of LAM suggests that estradiol contributes to disease development. Metabolomic profiling identified an estradiol-enhanced prostaglandin biosynthesis signature in Tsc2-deficient (TSC(-)) cells, both in vitro and in vivo. Estradiol increased the expression of cyclooxygenase-2 (COX-2), a rate-limiting enzyme in prostaglandin biosynthesis, which was also increased at baseline in TSC-deficient cells and was not affected by rapamycin treatment. However, both Torin 1 treatment and Rictor knockdown led to reduced COX-2 expression and phospho-Akt-S473. Prostaglandin production was also increased in TSC-deficient cells. In preclinical models, both Celecoxib and aspirin reduced tumor development. LAM patients had significantly higher serum prostaglandin levels than healthy women. 15-epi-lipoxin-A4 was identified in exhaled breath condensate from LAM subjects and was increased by aspirin treatment, indicative of functional COX-2 expression in the LAM airway. In vitro, 15-epi-lipoxin-A4 reduced the proliferation of LAM patient-derived cells in a dose-dependent manner. Targeting COX-2 and prostaglandin pathways may have therapeutic value in LAM and TSC-related diseases, and possibly in other conditions associated with mTOR hyperactivation. PMID:24395886

Li, Chenggang; Lee, Po-Shun; Sun, Yang; Gu, Xiaoxiao; Zhang, Erik; Guo, Yanan; Wu, Chin-Lee; Auricchio, Neil; Priolo, Carmen; Li, Jing; Csibi, Alfredo; Parkhitko, Andrey; Morrison, Tasha; Planaguma, Anna; Kazani, Shamsah; Israel, Elliot; Xu, Kai-Feng; Henske, Elizabeth Petri; Blenis, John; Levy, Bruce D; Kwiatkowski, David; Yu, Jane J

2014-01-13

111

A Sacrificial Lam: A Divided School Board, a Beleaguered Superintendent, and an Urgent Need to Improve Student Achievement  

ERIC Educational Resources Information Center

This case describes the confrontational relationship between four trustees on the San Antonio School Board and the San Antonio School District's superintendent Diana Lam, a nationally recognized school reformer, who came to San Antonio in 1994. The case includes a dramatic board meeting where a closely divided board meets to buy out Lam's…

Fossey, Richard

2011-01-01

112

Estradiol and mTORC2 cooperate to enhance prostaglandin biosynthesis and tumorigenesis in TSC2-deficient LAM cells  

PubMed Central

Lymphangioleiomyomatosis (LAM) is a progressive neoplastic disorder that leads to lung destruction and respiratory failure primarily in women. LAM is typically caused by tuberous sclerosis complex 2 (TSC2) mutations resulting in mTORC1 activation in proliferative smooth muscle–like cells in the lung. The female predominance of LAM suggests that estradiol contributes to disease development. Metabolomic profiling identified an estradiol-enhanced prostaglandin biosynthesis signature in Tsc2-deficient (TSC?) cells, both in vitro and in vivo. Estradiol increased the expression of cyclooxygenase-2 (COX-2), a rate-limiting enzyme in prostaglandin biosynthesis, which was also increased at baseline in TSC-deficient cells and was not affected by rapamycin treatment. However, both Torin 1 treatment and Rictor knockdown led to reduced COX-2 expression and phospho-Akt-S473. Prostaglandin production was also increased in TSC-deficient cells. In preclinical models, both Celecoxib and aspirin reduced tumor development. LAM patients had significantly higher serum prostaglandin levels than healthy women. 15-epi-lipoxin-A4 was identified in exhaled breath condensate from LAM subjects and was increased by aspirin treatment, indicative of functional COX-2 expression in the LAM airway. In vitro, 15-epi-lipoxin-A4 reduced the proliferation of LAM patient–derived cells in a dose-dependent manner. Targeting COX-2 and prostaglandin pathways may have therapeutic value in LAM and TSC-related diseases, and possibly in other conditions associated with mTOR hyperactivation. PMID:24395886

Li, Chenggang; Lee, Po-Shun; Sun, Yang; Gu, Xiaoxiao; Zhang, Erik; Guo, Yanan; Wu, Chin-Lee; Auricchio, Neil; Priolo, Carmen; Li, Jing; Csibi, Alfredo; Parkhitko, Andrey; Morrison, Tasha; Planaguma, Anna; Kazani, Shamsah; Israel, Elliot; Xu, Kai-Feng; Henske, Elizabeth Petri; Blenis, John; Levy, Bruce D.

2014-01-01

113

Laser Ablation Mass Spectrometer (LAMS) as a Standoff Analyzer in Space Missions for Airless Bodies  

NASA Technical Reports Server (NTRS)

A laser ablation mass spectrometer (LAMS) based on a time-of-flight (TOF) analyzer with adjustable drift length is proposed as a standoff elemental composition sensor for space missions to airless bodies. It is found that the use of a retarding potential analyzer in combination with a two-stage reflectron enables LAMS to be operated at variable drift length. For field-free drift lengths between 33 cm to 100 cm, at least unit mass resolution can be maintained solely by adjustment of internal voltages, and without resorting to drastic reductions in sensitivity. Therefore, LAMS should be able to be mounted on a robotic arm and analyze samples at standoff distances of up to several tens of cm, permitting high operational flexibility and wide area coverage of heterogeneous regolith on airless bodies.

Li, X.; Brinckerhoff, W. B.; Managadze, G. G.; Pugel, D. E.; Corrigan, C. M.; Doty, J. H.

2012-01-01

114

Polysaccharides of Berberis vulgaris  

Microsoft Academic Search

The inflorescence were collected in the period of mass flowering (May 31), and fruit and leaves in the phase of technical ripening of the fruit (September 28) in the environs of Ryazan' in 1981. The polysaccharides (PSs) from the air-dry raw material (moisture content of the flowers and leaves 9.2-10.2%, and of the fruit 10.1-11.0%) that had been twice extracted

E. G. Martynov; E. A. Stroev; D. D. Peskov

1984-01-01

115

Bionic lightweight design by laser additive manufacturing (LAM) for aircraft industry  

Microsoft Academic Search

Today many challenges lie ahead of the aircraft industry. The increasing competition and shortage of resources raise a challenge for future manufacturing technologies and lightweight design. A possibility to cope with these circumstances is the manufacturing technology of Laser Additive Manufacturing (LAM). However there are still challenges to cope with due to the processes novelty, such as the development of

Claus Emmelmann; Maren Petersen; Jannis Kranz; Eric Wycisk

2011-01-01

116

Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione level  

Microsoft Academic Search

Initiation of acetaminophen (APAP) toxicities is believed to be promoted by oxidative stress during the event of overdosage. The aim of the present study was to evaluate the hepatoprotective action of Moringa oleifera Lam (MO), an Asian plant of high medicinal value, against a single high dose of APAP. Groups of five male Sprague–Dawley rats were pre-administered with MO (200

S. Fakurazi; I. Hairuszah; U. Nanthini

2008-01-01

117

Low Profile Rigid UHF RFID Tags K. V. S. Rao, Sander F. Lam, Pavel V. Nikitin  

E-print Network

Low Profile Rigid UHF RFID Tags K. V. S. Rao, Sander F. Lam, Pavel V. Nikitin Intermec Technologies, we describe three passive UHF RFID tags designed for operating on various surfaces. One tag and rugged fully encapsulated passive UHF RFID tags are becoming a popular choice in many industrial RFID

Hochberg, Michael

118

Digital design of a Cryptographic card (LAM) embedded Smart Card Reader  

Microsoft Academic Search

This paper presents the design and implementation of a cryptographic card embedded smart card reader. The cryptographic card introduces a digital integrated encryption\\/decryption circuitry called LAM which is based on Peripheral Component Interconnect (PCI) Architecture for the use on a Personal Computer (PC) communication card. The implementation of the above hardware PC cryptography card has been designed using a Field

Panagiotis Margaronis; Lambrinoudakis Kostantinos; Gritzalis Stefanos

2007-01-01

119

(Analysis of MAssive Data in Earth and Universe Sciences) CeSAM -LAM  

E-print Network

researcher · Financial support « Investissement d'avenir » for a « Big Data » project. · Financial support and validate new hypothesis In the AMADEUS project : Visualisation of overall raw data using interactive dataAMADEUS (Analysis of MAssive Data in Earth and Universe Sciences) C. Surace, CeSAM - LAM S. Maabout

Canet, Léonie

120

New ?-glucosides of caffeoyl quinic acid from the leaves of Moringa oleifera Lam.  

PubMed

Two new caffeoyl quinic acid ?-glucosides, together with three known caffeoyl quinic acids and five known flavonoid glucosides, were isolated from the leaves of Moringa oleifera Lam. The structures of the new compounds were elucidated as 4-O-(4'-O-?-D-glucopyranosyl)-caffeoyl quinic acid (1) and 4-O-(3'-O-?-D-glucopyranosyl)-caffeoyl quinic acid (2) by spectroscopic analyses. PMID:21748486

Kashiwada, Yoshiki; Ahmed, Fakhruddin Ali; Kurimoto, Shin-ichiro; Kim, Sang-Yong; Shibata, Hirofumi; Fujioka, Toshihiro; Takaishi, Yoshihisa

2012-01-01

121

CRESCIMENTO INICIAL DE MORINGA ( Moringa oleifera Lam) SOB OMISSÃO DE NUTRIENTES  

Microsoft Academic Search

Moringa oleifera Lam is a leguminous species well adapted to arid and semi arid conditions and is largely used as ornamental for parks and gardens in the animal feeding and human diet and as medicinal. Since research informations on this plant species are scarce, the current work aimed to evaluate the production of leaves, stems and roots dry matter in

Hugo Vieira; Lucia Helena; Garófalo Chaves; Ricardo Almeida Viégas

122

FUNGAL COLONIZATION OF PHYLLOSPHERE AND LITTER OF QUERCUS ROTUNDIFOLIA LAM. IN A HOLM OAK FOREST (HIGH  

E-print Network

FUNGAL COLONIZATION OF PHYLLOSPHERE AND LITTER OF QUERCUS ROTUNDIFOLIA LAM. IN A HOLM OAK FOREST and litter leaves was studied at five different stages of decomposition using three different isolation from the litter layer. With increasing decomposition initial colonizers gradually disappear, being

Boyer, Edmond

123

Reproductive success of the endangered shrub Buxus balearica Lam. (Buxaceae): pollen limitation, and inbreeding and outbreeding  

E-print Network

Reproductive success of the endangered shrub Buxus balearica Lam. (Buxaceae): pollen limitation of habitat fragmentation raises the probability of pollination failure in a number of species, as both pollen pollen was limiting reproduc- tive success of the endangered shrub Buxus balea- rica in five populations

Traveset, Anna

124

A spatial light modulator for terahertz beams Wai Lam Chan,1  

E-print Network

an array of small terahertz devices that can independently control the transmission of a terahertz beamA spatial light modulator for terahertz beams Wai Lam Chan,1 Hou-Tong Chen,2 Antoinette J. Taylor,2 for terahertz beams using active terahertz metamaterials. Our first-generation device consists of a 4 4 pixel

Mittleman, Daniel

125

Removal of cadmium from aqueous system by shelled Moringa oleifera Lam. seed powder  

Microsoft Academic Search

The present study explores the unexploited sorption properties of the plant Moringa oleifera Lam. for decontamination of Cd at laboratory scale. Sorption studies using standard practices were carried out in batch experiments as functions of biomass dosage, contact time, metal concentrations, particle size and pH. Percentage sorption in each case was computed on the basis of Cd estimation using a

Parul Sharma; Pushpa Kumari; M. M. Srivastava; Shalini Srivastava

2006-01-01

126

Antifungal activity of crude extracts and essential oil of Moringa oleifera Lam  

Microsoft Academic Search

Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medicines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum, and Microsporum canis. GC–MS analysis of the chemical composition of the essential oil from leaves showed a total of 44 compounds. Isolated

Ping-Hsien Chuang; Chi-Wei Lee; Jia-Ying Chou; M. Murugan; Bor-Jinn Shieh; Hueih-Min Chen

2007-01-01

127

Short Communication Antifungal activity of crude extracts and essential oil of Moringa oleifera Lam  

Microsoft Academic Search

Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medi- cines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagro- phytes, Epidermophyton Xoccosum, and Microsporum canis. GC-MS analysis of the chemical composition of the essential oil from leaves showed a total of 44

Ping-Hsien Chuang; Chi-Wei Lee; Jia-Ying Chou; M. Murugan; Bor-Jinn Shieh; Hueih-Min Chen

128

Fatty acid, flavonol, and mineral composition variability among seven macrotyloma uniflorum (Lam.) verdc. accessions  

Technology Transfer Automated Retrieval System (TEKTRAN)

Horse gram [Macrotyloma uniflorum (Lam.) Verdc.] seeds containing high concentrations of fatty acids, flavonols and minerals will provide government, public and private organizations with a nutritious and healthy food for use by malnourished and food deprived people worldwide. Seeds from seven horse...

129

Analyze Influenza Virus Sequences Using Binary Encoding HamChing Lam  

E-print Network

Analyze Influenza Virus Sequences Using Binary Encoding Approach HamChing Lam Dept. of Computer 55455, USA boley@cs.umn.edu ABSTRACT Capturing mutation patterns of each individual influenza virus distance. We first convert the influenza genetic sequence to a binary string and then ap- ply Principal

Boley, Daniel

130

Catching Old Influenza Virus with A New Markov Model HamChing Lam  

E-print Network

Catching Old Influenza Virus with A New Markov Model HamChing Lam Dept. of Computer Science on the Hemag- glutinin (HA) gene, a major surface antigen of the avian influenza virus. Using this model we Influenza virus, Poisson process, Markov Model 1. INTRODUCTION For the past century researchers have been

Boley, Daniel

131

Using the Soot flow analysis framework Patrick Lam (plam@sable.mcgill.ca)  

E-print Network

in the control-flow graph. In Soot, we represent this data as flow sets. Typically, a flow set represents a setUsing the Soot flow analysis framework Patrick Lam (plam@sable.mcgill.ca) March 17, 2000 Slides from a talk on the Soot flow analysis framework are at http://www.sable.mcgill.ca/soot/notes. 1 Goals

Verbrugge, Clark

132

NASA FOIA PUBLIC LIAISON OFFICERS and REQUESTER SERVICE CENTERS Miriam Brown-Lam  

E-print Network

NASA FOIA PUBLIC LIAISON OFFICERS and REQUESTER SERVICE CENTERS Miriam Brown-Lam Principal Agency FOIA Officer Chief, NASA FOIA Public Liaison Officer (202) 358-0718 Josephine Sibley HQ FOIA Public Research Center (ARC) Lubna Shirzai ARC FOIA Public Liaison Officer NASA Ames Research Center Mail Stop 943

Waliser, Duane E.

133

Predicting weed migration from soil and climate maps. [Centaurea maculosa Lam  

Microsoft Academic Search

Soil characteristics, elevation, annual precipitation, potential evapotranspiration, length of frost-free season, and mean maximum July temperature were estimated for 116 established infestations of spotted knapweed (Centaurea maculosa Lam. number³ CENMA) in Montana using basic land resource maps. Areas potentially vulnerable to invasion by the plant were delineated on the basis of representative edaphic and climatic characteristics. No single environmental variable

T. K. Chicoine; P. K. Fay; G. A. Nielsen

1985-01-01

134

FDTD simulation of hexagonal micropillar cavities Frankie Kin Lam Tung, Ning Ma and Andrew W. Poon  

E-print Network

FDTD simulation of hexagonal micropillar cavities Frankie Kin Lam Tung, Ning Ma and Andrew W. Poon Hexagonal micropillar (µ-pillar) cavities have been studied using 2-D finite-difference time-domain (FDTD. Keywords: Hexagonal micro-pillar cavity, channel add-drop filter, waveguide-coupled, FDTD 1. INTRODUCTION

Poon, Andrew Wing On

135

Kinetics of Enol Formation from Reaction of OH with Propene Lam K. Huynh,,  

E-print Network

Kinetics of Enol Formation from Reaction of OH with Propene Lam K. Huynh,, Hongzhi R. Zhang*, Henry Eyring Center for Theoretical Chemistry, Department of Chemistry, UniVersity of Utah, Salt Lake City, Utah 84112, Department of Chemical Engineering, UniVersity of Utah, Salt Lake City, Utah 84112

Utah, University of

136

SQUASH BEETLE ON CUCURBITS Frankie Lam, Ricky E. Foster, Extension Entomologists,  

E-print Network

numbers of pest insects such as aphids. The exceptions are the squash beetle and the Mexican bean beetle. The squash beetle is an occasional pest on cucurbits, and the Mexican bean beetle is a common pest on beansSQUASH BEETLE ON CUCURBITS Frankie Lam, Ricky E. Foster, Extension Entomologists, Dan Egel

Ginzel, Matthew

137

Stochasticity in the Expression of LamB and its Affect on ? phage Infection  

NASA Astrophysics Data System (ADS)

? phage binds to E. Coli's lamB protein and injects its DNA into the cell. The phage quickly replicates and after a latent period the bacteria bursts, emitting mature phages. We developed a mathematical model based on the known physical events that occur when a ? phage infects an E.Coli cell. The results of these models predict that the bacteria and phage populations become extinct unless the parameters of the model are very finely tuned, which is untrue in the nature. The lamB protein is part of the maltose regulon and can be repressed to minimal levels when grown in the absence of inducer. Therefore, a cell that is not expressing any lamB protein at that moment is resistant against phage infection. We studied the dynamic relationship between ? phage and E. Coli when the concentration of phage greatly outnumbers the concentration of bacteria. We study how the stochasticity of the expression of lamB affects the percentage of cells that the ? phage infects. We show that even in the case when the maltose regulon is fully induced a percentage of cells continue to persist against phage infection.

Chapman, Emily; Wu, Xiao-Lun

2006-03-01

138

RFID Paperclip Tags Pavel V. Nikitin, K. V. S. Rao, and Sander Lam  

E-print Network

to attach standard flexible RFID inlays to the front page of a document [1]. Another existing approachRFID Paperclip Tags Pavel V. Nikitin, K. V. S. Rao, and Sander Lam Intermec Technologies.com Abstract-- In this paper, we present RFID tags which double as paperclips. These tags use standard metal

Hochberg, Michael

139

The use of Toddalia asiatica (L) Lam. (Rutaceae) in traditional medicine practice in East Africa  

Microsoft Academic Search

Toddalia asiatica (L) Lam. (Rutaceae) has been used by traditional health practitioners in East Africa for management of diseases, however, the extent of its usefulness has not been established to date. Fieldwork for this study was carried out in the Lake Victoria Basin between March and September 2006. The purpose was to collect ethnomedical information that will serve as a

J. A. Orwa; I. J. O. Jondiko; R. J. A. Minja; M. Bekunda

2008-01-01

140

Caihuan Ke K. N. Yu P. K. S. Lam Wen-Xiong Wang  

E-print Network

tissue dry weight of mussels. Introduction Development of nuclear facilities, including nuclear power may be present in the low level aqueous radioactive wastes discharged from nuclear fa- cilities@ust.hk Caihuan Ke á K. N. Yu á P. K. S. Lam Centre for Coastal Pollution and Conservation, City University

Yu, K.N.

141

The efficacy of the lactational amenorrhea method (LAM) among working women  

Microsoft Academic Search

The purpose of this study was to assess the efficacy of the lactational amenorrhea method (LAM) for family planning among mothers who are separated from their infants by work. The study population, 170 urban middle class women who planned to return to work before 120 days postpartum, were interviewed monthly for 6 months postpartum and contacted at 12 months. The

Verónica Valdés; Miriam H. Labbok; Edda Pugin; Alfredo Perez

2000-01-01

142

High fat diet induced insulin resistance and elevated retinol binding protein 4 in female rats; treatment and protection with Berberis vulgaris extract and vitamin A.  

PubMed

This research was conducted to investigate two main aims; the first aim was to find if there is a relationship between insulin resistance (IR) and retinol binding protein 4 (RBP4). The second aim was to use berberis vulgaris extract and vitamin A as protective and/or curative agents against insulin resistance. IR was developed by feeding the female rats a high fat diet (HFD) for six weeks then treating or protecting them with b. vulgaris extract (0.2 g/Kg body weight) or vitamin A (12.8?g/Kg/day) for two weeks. HFD intake elevated insulin level and RBP4 expression that associated with hyperglycemia and hyperlipidemia. Co-administration of vitamin A and B. vulgaris extracts reduced blood glucose level, insulin, body weight and RBP4 expression before, during and after HFD. Furthermore, vitamin A reduced the blood glucose, triglycerides (TG) and cholesterol levels. IR syndrome associated with the RBP 4 alteration that gives high indication about the role of RBP4 expression in the IR progression and development. Furthermore, the treatment with vitamin A and/or b. vulgaris alleviated the IR syndrome through the action on RBP4 and Insulin secretion. On the other hand, vitamin A must be avoided for the predisposed IR and prediabetic patients. PMID:24191325

El-Sayed, Mohamed Mohammed; Ghareeb, Doaa Ahmad; Talat, Heba Allah; Sarhan, Eman Mohammed

2013-11-01

143

Clonal expansion and seedling recruitment of Oregon grape (Berberis nervosa) in Douglas-fir (Pseudotsuga menziesii) forests: comparisons with salal (Gaultheria shallon)  

USGS Publications Warehouse

Seedling regeneration and morphology of Oregon grape (Berberis nervosa Pursh) and salal (Gaultheria shallon Pursh) were studied in thinned and unthinned Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) stands in the central Coast Range, Oregon. Above- and below-ground growth of both species were significantly and negatively correlated with stand density. Oregon grape appears to have less potential for vegetative spread than does salal. It produced two to three times fewer rhizome extensions, and rhizome extensions were only half as long as those of salal. Oregon grape seedlings were common in areas of moss ground cover among patches of the two species. Salal seedlings were restricted to decaying logs. Seedling densities of Oregon grape in thinned stands were more than six times those in unthinned stands. For Oregon grape, understory establishment is accomplished by seedling establishment and recruitment of new genets. In contrast, salal maintains itself in forest understories primarily through vegetative growth, since its seedling establishment is restricted mainly to decayed wood. Continual recruitment of new aerial stems or ramets enables Oregon grape to maintain a dense cover once it is established in the understory.

Huffman, D.; Tappeiner, J. C., II

1997-01-01

144

Inhibitory Effect of Berberis vulgaris Aqueous Extract on Acquisition and Reinstatement Effects of Morphine in Conditioned Place Preferences (CPP) in Mice  

PubMed Central

Background: It has been elucidated that Berberis vulgaris (barberry) can alleviate morphine withdrawal syndrome. Also it has been reported that aqueous extract of barberry possibly have inhibitory effect on NMDA receptors. Objectives: In this study, we decided to evaluate the effects of aqueous extract of B. vulgaris fruit on morphine tendency in mice using conditioned place preference (CPP) method. Materials and Methods: In experiment 1 (acquisition phase), mice underwent morphine-induced conditioned place preference (CPP) training with injections of morphine (40 mg/kg). In experiment 2 (extinction and reinstatement phases), mice underwent the same CPP training as in experiment 1 and subsequent extinction training on day 16th a reinstatement by CPP was done by injection of reminding 10 mg/kg morphine. Results: The administration of morphine (40 mg/kg for four days) produced place preference. In the first method, the aqueous extract of barberry (200 mg/kg) prevented morphine tendency to white cell in CPP method. In the second method, after inter-peritoneal injection of aqueous extracts of barberry at 100 and 200 mg/kg, the animals tendency toward the white cells of CPP chamber on the sixteenth day (after a reminder injection of morphine 10 mg/kg) was significantly reduced. Conclusions: These results show that aqueous extract of barberry can reduce the acquisition and reinstatement of morphine-induced conditioned place preference. PMID:25237645

Imenshahidi, Mohsen; Qaredashi, Reza; Hashemzaei, Mahmoud; Hosseinzadeh, Hossein

2014-01-01

145

Antidiabetic Effect of Methanolic Extract from Berberis julianae Schneid. via Activation of AMP-Activated Protein Kinase in Type 2 Diabetic Mice  

PubMed Central

We have investigated the antidiabetic effect and mechanism of methanolic extract of Berberis julianae Schneid. (BJSME) in STZ induced Type 2 diabetes mellitus mice. T2DM mice were induced by high fat diet and low dose streptozotocin (STZ). BJSME was orally administrated at the doses of 60, 120, and 240?mg/kg/d, for 21 days. Metformin was used as positive control drug. Food intake, body weight, plasma glucose, oral glucose tolerance test, insulin tolerance test, insulin, and blood-lipid content were measured. The effects of BJSME on the glucose transporter 4 (GLUT4) translocation in L6 myotubes and the GLUT4 protein expression in skeletal muscle as well as phosphorylation of the AMP-activated protein kinase (AMPK) in liver and muscle were examined. In vitro and in vivo results indicate that BJSME increased GLUT4 translocation by 1.8-fold and BJSME significantly improved the oral glucose tolerance and low density lipoprotein cholesterol (LDL-C) of serum and reduced body weight, glucose, and other related blood-lipid contents. The BJSME treatment also stimulated the phosphorylation of AMPK. Thus, BJSME seems to possess promising beneficial effects for the treatment of T2DM with the possible mechanism via stimulating AMPK activity. PMID:25258641

Yang, Jing; Zhao, Ping; Wan, Dingrong; Zhou, Qi; Wang, Chao; Shu, Guangwen; Mei, Zhinan; Yang, Xinzhou

2014-01-01

146

Embryo development from discrete cell aggregates in Ipomoea Batatas (L.) Lam. in response to structural polarity  

Microsoft Academic Search

Summary  High numbers of embryos are difficult to obtain in liquid cultures of sweet potato (Ipomoea batatas (L.) Lam.) because discrete cell aggregates, produced through calli fragmentation, do not support embryo growth. In an effort\\u000a to demonstrate that embryo development is possible from discrete cell aggregates, we compared embryo formation from cell aggregates\\u000a 250–355 ?m in diameter cultured either in suspension

Raymond P. Chée; Daniel J. Cantliffe

1989-01-01

147

New ?-glucosides of caffeoyl quinic acid from the leaves of Moringa oleifera Lam  

Microsoft Academic Search

Two new caffeoyl quinic acid ?-glucosides, together with three known caffeoyl quinic acids and five known flavonoid glucosides,\\u000a were isolated from the leaves of Moringa oleifera Lam. The structures of the new compounds were elucidated as 4-O-(4?-O-?-d-glucopyranosyl)-caffeoyl quinic acid (1) and 4-O-(3?-O-?-d-glucopyranosyl)-caffeoyl quinic acid (2) by spectroscopic analyses.

Yoshiki KashiwadaFakhruddin; Fakhruddin Ali Ahmed; Shin-ichiro Kurimoto; Sang-Yong Kim; Hirofumi Shibata; Toshihiro Fujioka; Yoshihisa Takaishi

148

Protective effects of echinocystic acid isolated from Gleditsia sinensis Lam. against acute myocardial ischemia  

Microsoft Academic Search

Echinocystic acid (EA), a pentacyclic triterpene, was isolated and identified from the fruits of Gleditsia sinensis Lam. The protective effects of EA were evaluated in rat models with acute myocardial ischemia induced by isoproterenol and vasopressin. In the electrocardiogram of anesthetized rats, EA prevented the ST-segment depression induced by isoproterenol or vasopressin in a dose-dependently manner. Furthermore, the mRNA expression

Jianming Wu; Jian Li; Zhiyong Zhu; Jiang Li; Guojun Huang; Yao Tang; Xiaoping Gao

2010-01-01

149

Anti-fungal activity of crude extracts and essential oil of Moringa oleifera Lam.  

PubMed

Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medicines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum, and Microsporum canis. GC-MS analysis of the chemical composition of the essential oil from leaves showed a total of 44 compounds. Isolated extracts could be of use for the future development of anti-skin disease agents. PMID:16406607

Chuang, Ping-Hsien; Lee, Chi-Wei; Chou, Jia-Ying; Murugan, M; Shieh, Bor-Jinn; Chen, Hueih-Min

2007-01-01

150

In vitro plant regeneration system for Cassia siamea Lam., a leguminous tree of economic importance  

Microsoft Academic Search

A method for rapid in vitro propagation of Cassia siamea Lam. using cotyledonary node explants, excised from 14-day old aseptic seedlings, has been established. Murashige and Skoog\\u000a (MS) medium supplemented with different concentrations of 6-benzyladenine (BA), kinetin (Kn) and thidiazuron (TDZ) singly\\u000a or in combination with auxins was used for regeneration studies. Among the single treatment of three cytokinins BA

Shahina Parveen; Anwar Shahzad; Syed Saema

2010-01-01

151

Evaluation of the effects of hydroalcoholic extract of Berberis vulgaris root on the activity of liver enzymes in male hypercholesterolemic rats  

PubMed Central

Objectives: Hyperlipidemia can cause a variety of diseases such as atherosclerosis, diabetes, and fatty liver which is followed by increased liver enzymes. Since Berberis vulgaris (B. vulgaris) root possesses antioxidant properties, the present study was conducted to investigate the effect of its extract on the activity of liver enzymes in rats. Materials and Methods: In this experimental study, sixty Wistar rats were selected and allocated to six groups of ten each. The control group received a normal diet and the sham group received a fatty diet while the other groups including experimental groups received a fatty diet and the alcoholic extract of B. vulgaris at minimum (75 mg/kg), moderate (150 mg/kg), and maximum (300 mg/kg) doses by intraperitoneal injection (i.p.) or oral atorvastatin (10 mg /kg) with a fatty diet. At the end of this 21-day period, blood samples were drawn and the levels of the intended factors were measured. Data were analyzed using SPSS software version 11.5. Results: The comparison of the obtained results showed that the levels of alanine transaminase (ALT) and alkaline phosphatase (ALP) enzymes in the sham group that only received fatty food increased (p?0.05), whereas in the treatment groups receiving B. vulgaris extract as well as in the group receiving Atorvastatin, these enzymes significantly decreased; however, no significant changes were observed in aspartate transaminase (AST) levels. Conclusion: Noticing the antioxidant properties of B. vulgaris root extract and its effects on reducing the activity of liver enzymes, the extract of this plant can be a good choice for improving the function of liver. PMID:25050245

Taheri, Soheila; Zarei, Ali; Changizi Ashtiyani, Saeed; Rezaei, Azam; Zaheiri, Saeed

2012-01-01

152

Preventive Effects of Aqueous Extract of Berberis integerrima Bge. Root on Liver Injury Induced by Diabetes Mellitus (Type 1) in Rats  

PubMed Central

This study was conducted to assessthe preventive effect of aqueous extract of Berberis integerrima Bge. root (AEBIR) on liver damage and oxidative stress induced by diabetes mellitus in rats. Forty male rats were divided into 5 groups as follows: 1- normal (N); 2- normal + barberry (N+B) (they received barberry root extract for 6 weeks); 3- diabetic (D) (they received Streptozotocin (STZ), 65 mg/Kg BW /i.p.); 4- diabetic +barberry before (D+Bb) (they received barberry root extract for 3 weeks before STZ injection and continued for another three weeks); and 5- diabetic + barberry after (D+Ba) (three days after STZ injection, they received barberry root extract for 3 weeks). The experimental groups received barberry root extract (500 mg/Kg bw) intra gastric by gavage for 6 weeks. The treatment of diabetic rats with AEBIR showed a significant decreases(p<0.001) in levels of blood glucose, malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin while body weight, total protein, superoxide dismutase (SOD), catalase(CAT) and reduced glutathione (GSH) increased (p<0.001) in comparison to diabetic control rats. Consumption of AEBIR in group D+Bb caused significant improvement in all these factors, compared to the group D+Ba. Also in this study, for the first time, we demonstrated that administration of AEBIR before diabetes induction resulted in enhanced amelioration of liver complications compared to the group receiving it after induction, indicating that AEBIR can play a preventive role in such patients. PMID:25561940

Ashraf, Hossein; Zare, Samad

2015-01-01

153

Determination of color, pigment, and phenolic stability in yogurt systems colored with nonacylated anthocyanins from Berberis boliviana L. as compared to other natural/synthetic colorants.  

PubMed

Anthocyanins are of interest to the food industry because of their antioxidant power, attractive color, and stability in high acid foods. Powder from the Peruvian berry Berberis boliviana Lechler, rich in nonacylated anthocyanins (7% to 8% dry weight), was incorporated into yogurt samples containing 3 different fat levels. Color (CIE L, a, b, chroma, and hue angle), pigment (monomeric anthocyanin and polymeric color), and total phenolics were monitored over 8 wk of storage and compared to yogurt treatments containing purple carrot acylated anthocyanins, red beet betalaines, or FD&C Red nr 40. Anthocyanin profiles were analyzed by HPLC coupled to photodiode array and mass detectors. Color of yogurt containing B. boliviana anthocyanins at 20 mg cyanidin-3-glucoside (cy-3-glu) equivalents/100 g yogurt (L*= 65, chroma = 14, and hue angle = 335 degrees ) was similar to commercial blueberry yogurt (L*= 65, chroma = 10.5, and hue angle = 341 degrees ). High color, pigment, and phenolic stability were observed in yogurts colored with B. boliviana, independent of the fat matrix. Acylated anthocyanins from purple carrot extracts exhibited increased stability with higher fat content. Anthocyanin degradation followed 1st-order kinetics. Pigment half-lives were 125 and 104 d for nonacylated anthocyanins at 10 and 20 mg cy-3-glu equivalents/100 g yogurt and 550.2, 232.6, and 128.9 d for acylated anthocyanins at 20 mg of cy-3-glu equivalents/100 g of 4%, 2%, and 0% fat yogurt. Addition of B. boliviana whole berry powder to yogurt matrices produced an attractive, stable anthocyanin-rich product, eliminating the need for industrial colorant extraction. PMID:18460117

Wallace, T C; Giusti, M M

2008-05-01

154

Preventive Effects of Aqueous Extract of Berberis integerrima Bge. Root on Liver Injury Induced by Diabetes Mellitus (Type 1) in Rats.  

PubMed

This study was conducted to assessthe preventive effect of aqueous extract of Berberis integerrima Bge. root (AEBIR) on liver damage and oxidative stress induced by diabetes mellitus in rats. Forty male rats were divided into 5 groups as follows: 1- normal (N); 2- normal + barberry (N+B) (they received barberry root extract for 6 weeks); 3- diabetic (D) (they received Streptozotocin (STZ), 65 mg/Kg BW /i.p.); 4- diabetic +barberry before (D+Bb) (they received barberry root extract for 3 weeks before STZ injection and continued for another three weeks); and 5- diabetic + barberry after (D+Ba) (three days after STZ injection, they received barberry root extract for 3 weeks). The experimental groups received barberry root extract (500 mg/Kg bw) intra gastric by gavage for 6 weeks. The treatment of diabetic rats with AEBIR showed a significant decreases(p<0.001) in levels of blood glucose, malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin while body weight, total protein, superoxide dismutase (SOD), catalase(CAT) and reduced glutathione (GSH) increased (p<0.001) in comparison to diabetic control rats. Consumption of AEBIR in group D+Bb caused significant improvement in all these factors, compared to the group D+Ba. Also in this study, for the first time, we demonstrated that administration of AEBIR before diabetes induction resulted in enhanced amelioration of liver complications compared to the group receiving it after induction, indicating that AEBIR can play a preventive role in such patients. PMID:25561940

Ashraf, Hossein; Zare, Samad

2015-01-01

155

16 Feature Article: Xiao-Feng Zhang, Chak-Man Lam and William K. Cheung Mining Local Data Sources For Learning Global  

E-print Network

16 Feature Article: Xiao-Feng Zhang, Chak-Man Lam and William K. Cheung Mining Local Data Sources For Learning Global Cluster Models Via Local Model Exchange Xiao-Feng Zhang, Chak-Man Lam, William K. Cheung basis. The orthogonal bases are then combined to give 1William K. Cheung is with Hong Kong Baptist

Cheung, William Kwok-Wai

156

A Job Pause Service under LAM/MPI+BLCR for Transparent Fault Tolerance  

SciTech Connect

Checkpoint/restart (C/R) has become a requirement for long-running jobs in large-scale clusters due to a meantime- to-failure (MTTF) in the order of hours. After a failure, C/R mechanisms generally require a complete restart of an MPI job from the last checkpoint. A complete restart, however, is unnecessary since all but one node are typically still alive. Furthermore, a restart may result in lengthy job requeuing even though the original job had not exceeded its time quantum. In this paper, we overcome these shortcomings. Instead of job restart, we have developed a transparent mechanism for job pause within LAM/MPI+BLCR. This mechanism allows live nodes to remain active and roll back to the last checkpoint while failed nodes are dynamically replaced by spares before resuming from the last checkpoint. Our methodology includes LAM/MPI enhancements in support of scalable group communicationwith fluctuating number of nodes, reuse of network connections, transparent coordinated checkpoint scheduling and a BLCR enhancement for job pause. Experiments in a cluster with the NAS Parallel Benchmark suite show that our overhead for job pause is comparable to that of a complete job restart. A minimal overhead of 5.6% is only incurred in case migration takes place while the regular checkpoint overhead remains unchanged. Yet, our approach alleviates the need to reboot the LAM run-time environment, which accounts for considerable overhead resulting in net savings of our scheme in the experiments. Our solution further provides full transparency and automation with the additional benefit of reusing existing resources. Executing continues after failures within the scheduled job, i.e., the application staging overhead is not incurred again in contrast to a restart. Our scheme offers additional potential for savings through incremental checkpointing and proactive diskless live migration, which we are currently working on.

Wang, Chao [North Carolina State University; Mueller, Frank [North Carolina State University; Engelmann, Christian [ORNL; Scott, Steven L [ORNL

2007-01-01

157

Phytochemical analysis, antimicrobial, antioxidant and urease inhibitory potential of Cyphostemma digitatum Lam.  

PubMed

In this paper we report the antimicrobial, antiradical and urease inhibitory potential along with photochemical investigation of the crude extracts of Cyphostemma digitatum Lam. Phytochemical screening of both the crude (hot/cold) alcoholic and aqueous extracts of C. digitatum showed the presence of alkaloids, flavonoids, saponins, coumarins, steroids, terpenoids and tannins. The crude methanolic extract (hot/cold) exhibited good antioxidant activity, while the aqueous extract was a weak antioxidant. The crude methanolic extract was found to be more active against Bacillus subtilis, while both the extracts showed moderate antifungal potential, the methanolic crude extract showed good urease inhibitory activity compared with the aqueous crude extract. PMID:25154866

Khan, Rasool; Saif, Abdullah Qasem; Quradha, Mohammad Mansour; Ali, Jawad; Rauf, Abdur

2015-01-01

158

Interaction of human laminin receptor with Sup35, the [PSI?] prion-forming protein from S. cerevisiae: a yeast model for studies of LamR interactions with amyloidogenic proteins.  

PubMed

The laminin receptor (LamR) is a cell surface receptor for extracellular matrix laminin, whereas the same protein within the cell interacts with ribosomes, nuclear proteins and cytoskeletal fibers. LamR has been shown to be a receptor for several bacteria and viruses. Furthermore, LamR interacts with both cellular and infectious forms of the prion protein, PrP(C) and PrP(Sc). Indeed, LamR is a receptor for PrP(C). Whether LamR interacts with PrP(Sc) exclusively in a capacity of the PrP receptor, or LamR specifically recognizes prion determinants of PrP(Sc), is unclear. In order to explore whether LamR has a propensity to interact with prions and amyloids, we examined LamR interaction with the yeast prion-forming protein, Sup35. Sup35 is a translation termination factor with no homology or functional relationship to PrP. Plasmids expressing LamR or LamR fused with the green fluorescent protein (GFP) were transformed into yeast strain variants differing by the presence or absence of the prion conformation of Sup35, respectively [PSI?] and [psi?]. Analyses by immunoprecipitation, centrifugal fractionation and fluorescent microscopy reveal interaction between LamR and Sup35 in [PSI?] strains. The presence of [PSI?] promotes LamR co-precipitation with Sup35 as well as LamR aggregation. In [PSI?] cells, LamR tagged with GFP or mCherry forms bright fluorescent aggregates that co-localize with visible [PSI?] foci. The yeast prion model will facilitate studying the interaction of LamR with amyloidogenic prions in a safe and easily manipulated system that may lead to a better understanding and treatment of amyloid diseases. PMID:24416454

Pampeno, Christine; Derkatch, Irina L; Meruelo, Daniel

2014-01-01

159

Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats.  

PubMed

This study was carried out to evaluate the effect of Moringa oleifera Lam (Moringa) seed extract on liver fibrosis. Liver fibrosis was induced by the oral administration of 20% carbon tetrachloride (CCl(4)), twice weekly and for 8 weeks. Simultaneously, M.oleifera Lam seed extract (1g/kg) was orally administered daily. The biochemical and histological results showed that Moringa reduced liver damage as well as symptoms of liver fibrosis. The administration of Moringa seed extract decreased the CCl(4)-induced elevation of serum aminotransferase activities and globulin level. The elevations of hepatic hydroxyproline content and myeloperoxidase activity were also reduced by Moringa treatment. Furthermore, the immunohistochemical study showed that Moringa markedly reduced the numbers of smooth muscle alpha-actin-positive cells and the accumulation of collagens I and III in liver. Moringa seed extract showed significant inhibitory effect on 1,1-diphenyl-2-picrylhydrazyl free radical, as well as strong reducing antioxidant power. The activity of superoxide dismutase as well as the content of both malondialdehyde and protein carbonyl, which are oxidative stress markers, were reversed after treatment with Moringa. Finally, these results suggested that Moringa seed extract can act against CCl(4)-induced liver injury and fibrosis in rats by a mechanism related to its antioxidant properties, anti-inflammatory effect and its ability to attenuate the hepatic stellate cells activation. PMID:19854235

Hamza, Alaaeldin A

2010-01-01

160

EFFECT OF ROOT TEMPERATURE ON SINK STRENGTH OF TUBEROUS ROOT IN SWEET POTATO PLANTS (IPOMOEA BATATAS LAM.)  

Microsoft Academic Search

EGUCHI T,. KITANO M. and EGUCHI H. Effect of root temperature on sink strength of tuberous root in sweet potato plants (Ipomoea batatas Lam.). BIOTRONICS 23, 75-80, 1994. Eflect of root temperature on sink strength of tuberous root in sweet potato plants was examined at root temperatures of 20 to 32°C under a constant air condition of 28°C and 70%

T. EGUCHI; M. KITANO; H. EGUCHI

161

Silk: A Resilient Routing Fabric for Peer-to-Peer Networks Simon S. Lam and Huaiyu Liu  

E-print Network

Silk: A Resilient Routing Fabric for Peer-to-Peer Networks£ Simon S. Lam and Huaiyu Liu Dept named Silk, will be referred to as Silk pro- tocols. From simulation experiments in which the number of concurrent joins and failures was up to 50% of the initial net- work size, we found that, for � ¾, Silk

Lam, Simon S.

162

ReTAX+: A Cooperative Taxonomy Revision Tool Sik Chun (Joey) Lam, Derek Sleeman, and Wamberto Vasconcelos  

E-print Network

1 ReTAX+: A Cooperative Taxonomy Revision Tool Sik Chun (Joey) Lam, Derek Sleeman, and Wamberto in an established taxonomy; it suggests a number of ways in which that can be achieved namely; modifying the new entity, the taxonomy, or both. Further, a set of refinement operators are used to guarantee

Vasconcelos, Wamberto

163

Low Cost Silver Ink RFID Tag Antennas Pavel V. Nikitin, Sander Lam, and K. V. S. Rao  

E-print Network

Low Cost Silver Ink RFID Tag Antennas Pavel V. Nikitin, Sander Lam, and K. V. S. Rao Intermec for manufacturing RFID tags at Ultra High Frequencies (UHF). An analysis of two different RFID tag an- tennas made from the results that RFID tag performance depends both on material and on the shape of the antenna

Hannaford, Blake

164

Digital Gene Expression Analysis Based on Integrated De Novo Transcriptome Assembly of Sweet Potato [Ipomoea batatas (L.) Lam.  

Microsoft Academic Search

BackgroundSweet potato (Ipomoea batatas L. [Lam.]) ranks among the top six most important food crops in the world. It is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. However, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. Hence, studies based

Xiang Tao; Ying-Hong Gu; Hai-Yan Wang; Wen Zheng; Xiao Li; Chuan-Wu Zhao; Yi-Zheng Zhang

2012-01-01

165

Identifying and selecting for genetic diversity in Papua New Guinea sweetpotato Ipomoea batatas (L.) Lam. germplasm collected as botanical seed  

Technology Transfer Automated Retrieval System (TEKTRAN)

Genetic analysis was conducted on 141 "Ipomoea batatas" L. (Lam.) Genotypes derived from botanical seed originally collected from 26 sites in 4 provinces in Papua New Guinea. Relatedness among accessions was estimated by analysis of the AFLP data using the Dice coefficient of similarity and UPGMA. ...

166

How to Automatically and Accurately Sandbox Microsoft IIS Wei Li Lap-chung Lam Tzi-cker Chiueh  

E-print Network

How to Automatically and Accurately Sandbox Microsoft IIS Wei Li Lap-chung Lam Tzi-cker Chiueh appli- cation against a sandboxing policy is a popular approach to detecting control-hijacking attack to the acceptance of this system call monitoring ap- proach is how to derive accurate sandboxing policies

Chiueh, Tzi-cker

167

Approximate String Matching in DNA Sequences Lok-Lam Cheng David W. Cheung Siu-Ming Yiu  

E-print Network

Approximate String Matching in DNA Sequences Lok-Lam Cheng David W. Cheung Siu-Ming Yiu Department,dcheung,smyiu}@csis.hku.hk Abstract Approximate string matching on large DNA sequences data is very important in bioinformatics. Some, our study find that suffix array is much bet- ter than suffix tree for indexing the DNA sequences

Cheung, David Wai-lok

168

Wideband Metal Mount UHF RFID Tag K. V. S. Rao, Sander F. Lam, and Pavel V. Nikitin*  

E-print Network

impedance matching for RFID chip. #12;The flexible antenna inlay was designed so that it could be placedWideband Metal Mount UHF RFID Tag K. V. S. Rao, Sander F. Lam, and Pavel V. Nikitin* Intermec.nikitin}@intermec.com Abstract This paper presents a wideband UHF RFID tag designed for operating on multiple materials including

Hannaford, Blake

169

Susceptibility of Italian ryegrasses (Lolium multiflorum Lam.) to Argentine stem weevil (Listronotus bonariensis (Kuschel)) feeding and oviposition  

Microsoft Academic Search

The susceptibility of Italian ryegrasses (Lolium multiflorum Lam.) to Argentine stem weevil (Listronotus bonariensis (Kuschel)) was investigated in laboratory feeding and oviposition experiments, and in a field trial. Some Italian ryegrasses (for example, ‘Concord’, ‘Corvette’, ‘Progrow’) are infected with an Acremonium-like endophytic fungus. The presence of Acremonium-like endophyte did not deter or reduce adult Argentine stem weevil feeding or oviposition.

R. A. Prestidge

1991-01-01

170

Chemical and sensory comparison of fresh and dried lulo (Solanum quitoense Lam.) fruit aroma.  

PubMed

The odour-active volatile compounds of lulo fruit (Solanum quitoense Lam.) were isolated by solvent extraction followed by solvent-assisted flavour evaporation (SAFE). GC-O and GCMS analyses as well as quantitation by internal standard method showed that (Z)-3-hexenal, ethyl butanoate, 3-sulphanylhexyl acetate, and ethyl hexanoate were key aroma compounds in this fruit. Other odorants with relevance because their contribution (high OAVs) to the overall aroma were 4-hydroxy-2,5-dimethyl-3(2H)-furanone, methyl benzoate, (E)-2-hexenal, and hexanal. Lulo fruit pulp in presence of maltodextrin DE-20 was dried by using four different types of drying methods: hot air-drying (HD), spray drying (SD), lyophilisation (LD), and ultrasonic convective hot air-drying (HUD). LD sample exhibited the highest sensory rank (lulo-like) in comparison with fresh fruit pulp. Hot-air drying processes (HD and HUD) changed adversely the aroma of lulo fruit pulp. PMID:25236202

Forero, Diana Paola; Orrego, Carlos Eduardo; Peterson, Devin Grant; Osorio, Coralia

2015-02-15

171

Isolation and characterization of a flocculating protein from Moringa oleifera Lam.  

PubMed

A flocculating protein from the seeds of Moringa oleifera Lam. was isolated by extraction with phosphate buffer followed by cation exchange chromatography. The molecular mass of the protein determined by SDS-PAGE was about 6.5 kDa, the isoelectric point was above pH 10. Amino acid analysis and sequencing showed high contents of glutamine, arginine and proline, and a total of 60 residues. The amino terminus is blocked by pyroglutamate. The flocculant capacity, determined in glass powder suspension, is comparable to that of a cationic polymer on polyacrylamide basis. Flocculation activity may be explained by the patch charge mechanism due to low molecular weight and high charge density. PMID:7727523

Gassenschmidt, U; Jany, K D; Tauscher, B; Niebergall, H

1995-04-13

172

Predicting weed migration from soil and climate maps. [Centaurea maculosa Lam  

SciTech Connect

Soil characteristics, elevation, annual precipitation, potential evapotranspiration, length of frost-free season, and mean maximum July temperature were estimated for 116 established infestations of spotted knapweed (Centaurea maculosa Lam. number/sup 3/ CENMA) in Montana using basic land resource maps. Areas potentially vulnerable to invasion by the plant were delineated on the basis of representative edaphic and climatic characteristics. No single environmental variable was an effective predictor of sites vulnerable to invasion by spotted knapweed. Only a combination of variables was effective, indicating that the factors that regulate adaptability of this plant are complex. This technique provides a first approximation map of the regions most similar environmentally to infested sites and; therefore, most vulnerable to further invasion. This weed migration prediction technique shows promise for predicting suitable habitats of other invader species. 6 references, 4 figures, 1 table.

Chicoine, T.K.; Fay, P.K.; Nielsen, G.A.

1985-01-01

173

Can Point-of-Care Urine LAM Strip Testing for Tuberculosis Add Value to Clinical Decision Making in Hospitalised HIV-Infected Persons?  

PubMed Central

Background The urine lipoarabinomannan (LAM) strip-test (Determine®-TB) can rapidly rule-in TB in HIV-infected persons with advanced immunosuppression. However, given high rates of empiric treatment amongst hospitalised patients in high-burden settings (?50%) it is unclear whether LAM can add any value to clinical decision making, or identify a subset of patients with unfavourable outcomes that would otherwise have been missed by empiric treatment. Methods 281 HIV-infected hospitalised patients with suspected TB received urine LAM strip testing, and were categorised as definite (culture-positive), probable-, or non-TB. Both the proportion and morbidity of TB cases identified by LAM testing, early empiric treatment (initiated prior to test result availability) and a set of clinical predictors were compared across groups. Results 187/281 patients had either definite- (n?=?116) or probable-TB (n?=?71). As a rule-in test for definite and probable-TB, LAM identified a similar proportion of TB cases compared to early empiric treatment (85/187 vs. 93/187, p?=?0.4), but a greater proportion than classified by a set of clinical predictors alone (19/187; p<0.001). Thirty-nine of the 187 (21%) LAM-positive patients who had either definite- or probable-TB were missed by early empiric treatment, and of these 25/39 (64%) would also have been missed by smear microscopy. Thus, 25/187 (8%) of definite- or probable-TB patients with otherwise delayed initiation of TB treatment could be detected by the LAM strip test. LAM-positive patients missed by early empiric treatment had a lower median CD4 count (p?=?0.008), a higher median illness severity score (p?=?0.001) and increased urea levels (p?=?0.002) compared to LAM-negative patients given early empiric treatment. Conclusions LAM strip testing outperformed TB diagnosis based on clinical criteria but in day-to-day practice identified a similar proportion of patients compared to early empiric treatment. However, compared to empiric treatment, LAM identified a different subset of patients with more advanced immunosuppression and greater disease severity. PMID:23390504

Peter, Jonathan G.; Theron, Grant; Dheda, Keertan

2013-01-01

174

THE APPLICATION OF LASER.ABLATION MICROPROBE - INDUCTIVELY COU PLED PLASMA - MASS SPECTROMETRY (LAM-ICP-MS) TO IN SITU TRACE.ELEMENT DETERMINATIONS IN MINERALS  

Microsoft Academic Search

A laser ablation microprobe (LAM) sample-introduction system, designed for in sira microsampling of minerals in petrographic sections, has been interfacedto anlnductively CoupledPlasma-Mass Spectrometer(ICP-MS). TheLAM consists of aQ-switched Nd:YAG laser with power attenuation and steering optics to guide the laser beam through the phototube of a petrographic microscope, where it is focused onto the petrographic section contained in a sample cell.

SMON E. JACKSON; HENRY P. LONGERICH; GREG R. DUNNING; BRIAN J. FRYER

175

Structural and biochemical characterization of the laminarinase ZgLamCGH16 from Zobellia galactanivorans suggests preferred recognition of branched laminarin.  

PubMed

Laminarin is a ?-1,3-D-glucan displaying occasional ?-1,6 branches. This storage polysaccharide of brown algae constitutes an abundant source of carbon for marine bacteria such as Zobellia galactanivorans. This marine member of the Bacteroidetes possesses five putative ?-1,3-glucanases [four belonging to glycosyl hydrolase family 16 (GH16) and one to GH64] with various modular architectures. Here, the characterization of the ?-glucanase ZgLamC is reported. The catalytic GH16 module (ZgLamCGH16) was produced in Escherichia coli and purified. This recombinant enzyme has a preferential specificity for laminarin but also a significant activity on mixed-linked glucan (MLG). The structure of an inactive mutant of ZgLamCGH16 in complex with a thio-?-1,3-hexaglucan substrate unravelled a straight active-site cleft with three additional pockets flanking subsites -1, -2 and -3. These lateral pockets are occupied by a glycerol, an acetate ion and a chloride ion, respectively. The presence of these molecules in the vicinity of the O6 hydroxyl group of each glucose moiety suggests that ZgLamCGH16 accommodates branched laminarins as substrates. Altogether, ZgLamC is a secreted laminarinase that is likely to be involved in the initial step of degradation of branched laminarin, while the previously characterized ZgLamA efficiently degrades unbranched laminarin and oligo-laminarins. PMID:25664729

Labourel, Aurore; Jam, Murielle; Legentil, Laurent; Sylla, Balla; Hehemann, Jan Hendrik; Ferrières, Vincent; Czjzek, Mirjam; Michel, Gurvan

2015-02-01

176

Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats  

Microsoft Academic Search

The leaves of Moringa oleifera Lam (Moringaceae) are used by the Indians in their herbal medicine as a hypocholesterolemic agent in obese patients. The scientific basis for their use in hypercholesterolemia was therefore examined. It was found that administration of the crude leaf extract of Moringa oleifera along with high-fat diet decreased the high-fat diet-induced increases in serum, liver, and

S Ghasi; E Nwobodo; J. O Ofili

2000-01-01

177

EFECTO PERJUDICIAL DE Moringa oleifera (Lam.) COMBINADA CON OTROS DESECHOS AGRÍCOLAS COMO SUSTRATOS PARA LA LOMBRIZ ROJA (Eisenia spp.)  

Microsoft Academic Search

SUMMARY An experiment was carried out to study the behavior of the red worm (Eisenia spp.) fed with 5 nutritious substrates based in Moringa oleifera (Lam.) foliage, for the nutritional potentiali- ties, and other agricultural wastes in Trujillo state, Venezuela. A randomized design with repeated measurements and three repli- cates was used. Initially, 1.16kg of biomass in 0.5m3 of substrate

Luis José Cov; Danny Eugenio García; Alexander Rafael Castro; María Gabriela Medina

2007-01-01

178

Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees  

Microsoft Academic Search

Barks extracts of four different trees (Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam.) in three different solvents 80% methanol, 80% ethanol, and 80% acetone (solvent:water, 80:20v\\/v) were evaluated for their antioxidant activity, total phenolic (TP), and total flavonoids (TF) contents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system and

Bushra Sultana; Farooq Anwar; Roman Przybylski

2007-01-01

179

A valued Indian medicinal plant - Begonia malabarica Lam. : Successful plant regeneration through various explants and field performance  

Microsoft Academic Search

A cost-effective and efficient protocol has been described in the present work for large- scale and rapid in vitro propagation of a valuable medicinal herb Begonia malabarica Lam. (Begoniaceae) by shoot auxillary-bud proliferation and organogenesis on MS medium supplemented with 6-benzylaminopurine (BA; 0.0-8.8 mg\\/l) and indole-3-acetic acid (IAA; 0.0-2.88 mg\\/l) at different concentrations, either alone or in combinations. Initiation of

Mathan C. Nisha; Sevanan Rajeshkumar; Thangavel Selvaraj

180

Trace element partition coefficients for clinopyroxene and phlogopite in an alkaline lamprophyre from Newfoundland by LAM-ICP-MS  

Microsoft Academic Search

Mineral\\/rock matrix partition coefficients have been determined for clinopyroxene (Cpx) and phlogopite from a Mesozoic alkaline lamprophyre from Newfoundland, Canada, by Laser Ablation Microprobe (LAM-ICP-MS). Values for twenty-one elements were obtained for Cpx, whereas only eleven were possible for phlogopite due to REE abundances below detection limits ( 1 ppm). Ablation pits of 40–50 ?m diameter enabled investigation of zonation

Stephen F. Foley; Simon E. Jackson; Brian J. Fryer; John D. Greenouch; George A. Jenner

1996-01-01

181

In Vitro Wound Healing Potential and Identification of Bioactive Compounds from Moringa oleifera Lam  

PubMed Central

Moringa oleifera Lam. (M. oleifera) from the monogeneric family Moringaceae is found in tropical and subtropical countries. The present study was aimed at exploring the in vitro wound healing potential of M. oleifera and identification of active compounds that may be responsible for its wound healing action. The study included cell viability, proliferation, and wound scratch test assays. Different solvent crude extracts were screened, and the most active crude extract was further subjected to differential bioguided fractionation. Fractions were also screened and most active aqueous fraction was finally obtained for further investigation. HPLC and LC-MS/MS analysis were used for identification and confirmation of bioactive compounds. The results of our study demonstrated that aqueous fraction of M. oleifera significantly enhanced proliferation and viability as well as migration of human dermal fibroblast (HDF) cells compared to the untreated control and other fractions. The HPLC and LC-MS/MS studies revealed kaempferol and quercetin compounds in the crude methanolic extract and a major bioactive compound Vicenin-2 was identified in the bioactive aqueous fraction which was confirmed with standard Vicenin-2 using HPLC and UV spectroscopic methods. These findings suggest that bioactive fraction of M. oleifera containing Vicenin-2 compound may enhance faster wound healing in vitro. PMID:24490175

Muhammad, Abubakar Amali; Pauzi, Nur Aimi Syarina; Arulselvan, Palanisamy; Abas, Faridah; Fakurazi, Sharida

2013-01-01

182

Hybridization and invasion: an experimental test with diffuse knapweed (Centaurea diffusa Lam.)  

PubMed Central

A number of studies have suggested a link between hybridization and invasion. In this study, we experimentally test the potential for hybridization to influence invasion through a greenhouse common garden study. Diffuse knapweed (DK) (Centaurea diffusa Lam.) was introduced to North America with admixture from spotted knapweed (SK) (Centaurea stoebe subsp. stoebe L.). Comparisons between North American DK (including hybrid phenotypes) and native (European) DK in a common garden did not reveal enhanced performance or increased phenotypic variance, suggesting that pre-introduction hybridization or, more generally, post-introduction evolutionary change has not significantly contributed to the invasion of DK. In contrast, early generation hybrids [artificially created Backcross 1 (BC1) plants] exhibited increased variance for eight of the examined traits, and greater leaf and reproductive shoot production when compared to North American DK. Individual BC1 lines differed for several traits, suggesting the importance of the cross for drawing conclusions from such comparisons. When compared to the parental species (DK and SK), the BC1 plants were not transgressive for any of the measured traits. Overall, these findings suggest that if diploid SK is introduced to North America, interspecific hybridization has the potential to result in even more aggressive invaders. PMID:25568026

Blair, Amy C; Blumenthal, Dana; Hufbauer, Ruth A

2012-01-01

183

Hybridization and invasion: an experimental test with diffuse knapweed (Centaurea diffusa Lam.).  

PubMed

A number of studies have suggested a link between hybridization and invasion. In this study, we experimentally test the potential for hybridization to influence invasion through a greenhouse common garden study. Diffuse knapweed (DK) (Centaurea diffusa Lam.) was introduced to North America with admixture from spotted knapweed (SK) (Centaurea stoebe subsp. stoebe L.). Comparisons between North American DK (including hybrid phenotypes) and native (European) DK in a common garden did not reveal enhanced performance or increased phenotypic variance, suggesting that pre-introduction hybridization or, more generally, post-introduction evolutionary change has not significantly contributed to the invasion of DK. In contrast, early generation hybrids [artificially created Backcross 1 (BC1) plants] exhibited increased variance for eight of the examined traits, and greater leaf and reproductive shoot production when compared to North American DK. Individual BC1 lines differed for several traits, suggesting the importance of the cross for drawing conclusions from such comparisons. When compared to the parental species (DK and SK), the BC1 plants were not transgressive for any of the measured traits. Overall, these findings suggest that if diploid SK is introduced to North America, interspecific hybridization has the potential to result in even more aggressive invaders. PMID:25568026

Blair, Amy C; Blumenthal, Dana; Hufbauer, Ruth A

2012-01-01

184

First chemical constituents from Cordia exaltata Lam and antimicrobial activity of two neolignans.  

PubMed

The phytochemical study of Cordia exaltata Lam. (Boraginaceae) led to the isolation, through chromatographic techniques, of nineteen secondary metabolites: 8,8'dimethyl-3,4,3',4'-dimethylenedioxy-7-oxo-2,7'cyclolignan (1), 8,8'-dimethyl-4,5-dimethoxy-3',4'-methylenodioxy-7-oxo-2,7'cyclolignan (2), sitosterol (3a), stigmasterol (3b), sitosterol-3-O-?-D-glucopyranoside (4a), stigmasterol-3-O-?-D-glucopyranoside (4b), phaeophytin A (5), 13²-hydroxyphaeophytin A (6), 17³-ethoxypheophorbide A (7), 13²-hydroxy-17³-ethoxypheophorbide A (8), m-methoxy-p-hydroxybenzaldehyde (9), (E)-7-(3,4-dihydroxyphenyl)-7-propenoic acid (10), 1-benzopyran-2-one (11), 7-hydroxy-1-benzopyran-2-one (12), 2,5-bis-(3',4'-methylenedioxiphenyl)-3,4-dimethyltetrahydrofuran (13), 3,4,5,3',5'-pentamethoxy-1'-allyl-8.O.4'-neolignan (14), 3,5,7,3',4'-pentahydroxyflavonol (15), 5,7-dihydroxy-4'-methoxyflavone (16), 5,8-dihydroxy-7,4'-dimethoxyflavone (17), kaempherol 3-O-?-D-glucosyl-6''-?-L-ramnopyranoside (18) and kaempherol 3,7-di-O-?-L-ramnopyranoside (19). Their structures were identified by ¹H and ¹³C-NMR using one and two-dimensional techniques. In addition, the antimicrobial activity of compounds 1, 2, 13 and 14 against bacteria and fungi are reported here for the first time. PMID:24025457

de Sá de Sousa Nogueira, Tiago Bezerra; de Sá de Sousa Nogueira, Raquel Bezerra; E Silva, Davi Antas; Tavares, Josean Fechine; de Oliveira Lima, Edeltrudes; de Oliveira Pereira, Fillipe; da Silva Maciel, Jéssica Karina; de Souza Fernandes, Milen Maria Magalhães; de Medeiros, Fernando Antônio; do Socorro Ferreira Rodrigues Sarquis, Rosangela; Filho, Raimundo Braz; de Fátima Vanderlei de Souza, Maria

2013-01-01

185

Disentangling the Origins of Cultivated Sweet Potato (Ipomoea batatas (L.) Lam.)  

PubMed Central

Sweet potato (Ipomoea batatas (L.) Lam., Convolvulaceae) counts among the most widely cultivated staple crops worldwide, yet the origins of its domestication remain unclear. This hexaploid species could have had either an autopolyploid origin, from the diploid I. trifida, or an allopolyploid origin, involving genomes of I. trifida and I. triloba. We generated molecular genetic data for a broad sample of cultivated sweet potatoes and its diploid and polyploid wild relatives, for noncoding chloroplast and nuclear ITS sequences, and nuclear SSRs. Our data did not support an allopolyploid origin for I. batatas, nor any contribution of I. triloba in the genome of domesticated sweet potato. I. trifida and I. batatas are closely related although they do not share haplotypes. Our data support an autopolyploid origin of sweet potato from the ancestor it shares with I. trifida, which might be similar to currently observed tetraploid wild Ipomoea accessions. Two I. batatas chloroplast lineages were identified. They show more divergence with each other than either does with I. trifida. We thus propose that cultivated I. batatas have multiple origins, and evolved from at least two distinct autopolyploidization events in polymorphic wild populations of a single progenitor species. Secondary contact between sweet potatoes domesticated in Central America and in South America, from differentiated wild I. batatas populations, would have led to the introgression of chloroplast haplotypes of each lineage into nuclear backgrounds of the other, and to a reduced divergence between nuclear gene pools as compared with chloroplast haplotypes. PMID:23723970

Roullier, Caroline; Duputié, Anne; Wennekes, Paul; Benoit, Laure; Fernández Bringas, Víctor Manuel; Rossel, Genoveva; Tay, David; McKey, Doyle; Lebot, Vincent

2013-01-01

186

Purification and structural characterization of an ?-glucosidase inhibitory polysaccharide from apricot (Armeniaca sibirica L. Lam.) pulp.  

PubMed

In this study, the crude polysaccharide (APPS) from the fruiting bodies of apricot (Armeniaca sibirica L. Lam.) was isolated and fractionated by ultrafiltration and Sephadex G-75 gel chromatography. The hypoglycemic activities of all fractions were determined by ?-glucosidase inhibitory activity in vitro. The fraction APPS1-2 showed the best activity with an IC50 of 6.06mg/mL. The properties and chemical compositions of this fraction were analyzed with high-performance liquid chromatography, gel permeation chromatography-eighteen angle laser light scattering instrument, UV spectroscopy, infrared spectroscopy, and NMR spectroscopy ((1)H). The results demonstrated that APPS1-2 was a neutral glycoconjugate with a molecular weight of 25.93kDa. It comprised rhamnose, glucose, mannose, and galactose, with a relative molar ratio of 1.34:2.01:0.48:0.35. The backbone of APPS1-2 may consist of rhamnose and glucose, but its branches may consist of mannose and galactose. The IR and UV spectrum of APPS1-2 revealed the typical characteristics of heteropolysaccharide. (1)H NMR spectrum showed that APPS1-2 contained ?-configurations. PMID:25659703

Cui, Jie; Gu, Xin; Wang, Fengjun; Ouyang, Jie; Wang, Jianzhong

2015-05-01

187

Removal of cadmium from aqueous system by shelled Moringa oleifera Lam. seed powder.  

PubMed

The present study explores the unexploited sorption properties of the plant Moringa oleifera Lam. for decontamination of Cd at laboratory scale. Sorption studies using standard practices were carried out in batch experiments as functions of biomass dosage, contact time, metal concentrations, particle size and pH. Percentage sorption in each case was computed on the basis of Cd estimation using a planar NaI (TI) detector coupled to a 4K MCA (Canberra Accuspec Card with PC-AT 386). The adsorption data accurately in a Freundlich isotherm. Sorption studies resulted in the standardization of optimum conditions for removal of Cd (85.10%) as follows: biomass dosage (4.0 g), metal concentration (25 microg/ml), contact time (40 min) and volume of the test solution (200 ml) at pH 6.5. Fourier transform infrared (FTIR) spectrometry highlighted amino acid-Cd interactions responsible for sorption phenomenon. The findings open up new avenues in the removal of toxic metals by shelled Moringa oleifera seeds (SMOS) from water bodies as low cost, domestic and environmentally friendly safe technology. PMID:15949938

Sharma, Parul; Kumari, Pushpa; Srivastava, M M; Srivastava, Shalini

2006-01-01

188

Achyrocline satureioides (Lam.) D.C. Hydroalcoholic Extract Inhibits Neutrophil Functions Related to Innate Host Defense  

PubMed Central

Achyrocline satureioides (Lam.) D.C. is a herb native to South America, and its inflorescences are popularly employed to treat inflammatory diseases. Here, the effects of the in vivo actions of the hydroalcoholic extract obtained from inflorescences of A. satureioides on neutrophil trafficking into inflamed tissue were investigated. Male Wistar rats were orally treated with A. satureioides extract, and inflammation was induced one hour later by lipopolysaccharide injection into the subcutaneous tissue. The number of leukocytes and the amount of chemotactic mediators were quantified in the inflammatory exudate, and adhesion molecule and toll-like receptor 4 (TLR-4) expressions and phorbol-myristate-acetate- (PMA-) stimulated oxidative burst were quantified in circulating neutrophils. Leukocyte-endothelial interactions were quantified in the mesentery tissue. Enzymes and tissue morphology of the liver and kidney were evaluated. Treatment with A. satureioides extract reduced neutrophil influx and secretion of leukotriene B4 and CINC-1 in the exudates, the number of rolling and adhered leukocytes in the mesentery postcapillary venules, neutrophil L-selectin, ?2-integrin and TLR-4 expression, and oxidative burst, but did not cause an alteration in the morphology and activities of liver and kidney. Together, the data show that A. satureioides extract inhibits neutrophil functions related to the innate response and does not cause systemic toxicity. PMID:23476704

Barioni, Eric Diego; Machado, Isabel Daufenback; Rodrigues, Stephen Fernandes de Paula; Ferraz-de-Paula, Viviane; Wagner, Theodoro Marcel; Cogliati, Bruno; Corrêa dos Santos, Matheus; Machado, Marina da Silva; de Andrade, Sérgio Faloni; Niero, Rivaldo; Farsky, Sandra Helena Poliselli

2013-01-01

189

The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication  

PubMed Central

Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus. PMID:25767554

Husin, Fitrien; Chan, Yean Yean; Gan, Siew Hua; Sulaiman, Siti Amrah; Shueb, Rafidah Hanim

2015-01-01

190

Protective effects of Moringa oleifera Lam. leaves against arsenic-induced toxicity in mice  

PubMed Central

Objective To evaluate the protective role of leaves of Moringa oleifera (M. oleifera) Lam. against arsenic-induced toxicity in mice. Methods Swiss albino male mice were divided into four groups. The first group was used as non-treated control group while, the second, third, and fourth groups were treated with M. oleifera leaves (50 mg/kg body weight per day), sodium arsenite (10 mg/kg body weight per day) and sodium arsenite plus M. oleifera leaves, respectively. Serum indices related to cardiac, liver and renal functions were analyzed to evaluate the protective effect of Moringa leaves on arsenic-induced effects in mice. Results It revealed that food supplementation of M. oleifera leaves abrogated the arsenic-induced elevation of triglyceride, glucose, urea and the activities of alkaline phospatase, aspartate aminotransferase and alanine aminotransferase in serum. M. oleifera leaves also prevented the arsenic-induced perturbation of serum butyryl cholinesterase activity, total cholesterol and high density lipoprotein cholesterol. Conclusions The results indicate that the leaves of M. oleifera may be useful in reducing the effects of arsenic-induced toxicity. PMID:25183111

Sheikh, Afzal; Yeasmin, Fouzia; Agarwal, Smita; Rahman, Mashiur; Islam, Khairul; Hossain, Ekhtear; Hossain, Shakhawoat; Karim, Md Rezaul; Nikkon, Farjana; Saud, Zahangir Alam; Hossain, Khaled

2014-01-01

191

Simultaneous HPLC quantitative analysis of active compounds in leaves of Moringa oleifera Lam.  

PubMed

Moringa oleifera Lam. has been used as a traditional medicine for the treatment of numerous diseases. A simultaneous high-performance liquid chromatography (HPLC) analysis was developed and validated for the determination of the contents of crypto-chlorogenic acid, isoquercetin and astragalin, the primary antioxidative compounds, in M. oleifera leaves. HPLC analysis was successfully conducted by using a Hypersil BDS C18 column, eluted with a gradient of methanol-1% acetic acid with a flow rate of 1 mL/min, and detected at 334 nm. Parameters for the validation included linearity, precision, accuracy and limits of detection and quantitation. The developed HPLC method was precise, with relative standard deviation < 2%. The recovery values of crypto-chlorogenic acid, isoquercetin and astragalin in M. oleifera leaf extracts were 98.50, 98.47 and 98.59%, respectively. The average contents of these compounds in the dried ethanolic extracts of the leaves of M. oleifera collected from different regions of Thailand were 0.081, 0.120 and 0.153% (w/w), respectively. The developed HPLC method was appropriate and practical for the simultaneous analysis of crypto-chlorogenic acid, isoquercetin and astragalin in the leaf extract of M. oleifera. This work is valuable as guidance for the standardization of the leaf extracts and pharmaceutical products of M. oleifera. PMID:23828911

Vongsak, Boonyadist; Sithisarn, Pongtip; Gritsanapan, Wandee

2014-08-01

192

Gene Expression Rhythms in the Mussel Mytilus galloprovincialis (Lam.) across an Annual Cycle  

PubMed Central

Seasonal environmental changes may affect the physiology of Mytilus galloprovincialis (Lam.), an intertidal filter-feeder bivalve occurring commonly in Mediterranean and Atlantic coastal areas. We investigated seasonal variations in relative transcript abundance of the digestive gland and the mantle (gonads) of males and females. To identify gene expression trends – in terms of relative mRNA abundance- we used a medium-density cDNA microarray (1.7 K probes) in dual-color competitive hybridization analyses. Hierarchical clustering of digestive gland microarray data showed two main branches, distinguishing profiles associated with the “hot” months (May–August) from the other months. Genes involved in chitin metabolism, associated with mussel nutrition and digestion showed higher mRNA levels during summer. Moreover, we found different gene transcriptomic patterns in the digestive glands of males when compared to females, during the four stages of mussel gonadal development. Microarray data from gonadal transcripts also displayed clear patterns during the different developmental phases respect to the resting period (stage I) with peak relative mRNA abundance at the ripe phase (stage III) for both sexes. These data showed a clear temporal pattern in transcriptomic profiles of mussels sampled over an annual cycle. Physiological response to thermal variation, food availability, and reproductive status across months may contribute to variation in relative mRNA abundance. PMID:21573210

Banni, Mohamed; Negri, Alessandro; Mignone, Flavio; Boussetta, Hamadi; Viarengo, Aldo; Dondero, Francesco

2011-01-01

193

Investigation on the influence of foreign metal ions in crystal growth and characterization of L-Alaninium Maleate (LAM) single crystals  

NASA Astrophysics Data System (ADS)

A Nonlinear Optical, good quality, single crystals of doped and undoped L-Alaninium Maleate (LAM) were grown by slow evaporation solution growth technique at room temperature. The lattice parameters were analyzed by single crystal X-ray diffraction technique. The identification of Cadmium ion in the doped crystals was done using the EDAX spectrum. The presence of functional group of the dopant with LAM molecule was studied using FTIR spectra. The results of UV-Vis study is used to compare the transparencies of the doped and undoped LAM crystals. The optical band gap energy of the grown crystal was also calculated. The relative second harmonic generation (SHG) efficiency measurement with KDP reference is used to find the incorporation of metal to L-Alaninium Maleate crystals and the parent material. Also the thermal stability of the grown crystals was studied by TGA/DTA spectrum. The mechanical stability of the grown crystals was confirmed through Vickers micro hardness study. By parallel plate capacitor technique, the dielectric response was studied over a wide range of frequencies at different temperatures. The various studies showed the incorporation of the impurity Cd2+ into LAM crystals and the investigations indicated that the impurity played an important role in the changes of the spectral and structural properties of LAM crystals.

Ruby Nirmala, L.; Thomas Joseph Prakash, J.

2013-11-01

194

Transcriptional response of the mussel Mytilus galloprovincialis (Lam.) following exposure to heat stress and copper.  

PubMed

Global warming is a major factor that may affect biological organization, especially in marine ecosystems and in coastal areas that are particularly subject to anthropogenic pollution. We evaluated the effects of simultaneous changes in temperature and copper concentrations on lysosomal membrane stability (N-acetyl-hexosaminidase activity) and malondialdehyde accumulation (MDA) in the gill of the blue mussel Mytilus galloprovincialis (Lam.). Temperature and copper exerted additive effects on lysosomal membrane stability, exacerbating the toxic effects of metal cations present in non-physiological concentrations. Mussel lysosomal membrane stability is known to be positively related to scope for growth, indicating possible effects of increasing temperature on mussel populations in metal-polluted areas. To clarify the molecular response to environmental stressors, we used a cDNA microarray with 1,673 sequences to measure the relative transcript abundances in the gills of mussels exposed to copper (40 µg/L) and a temperature gradient (16°C, 20°C, and 24°C). In animals exposed only to heat stress, hierarchical clustering of the microarray data revealed three main clusters, which were largely dominated by down-regulation of translation-related differentially expressed genes, drastic up-regulation of protein folding related genes, and genes involved in chitin metabolism. The response of mussels exposed to copper at 24°C was characterized by an opposite pattern of the genes involved in translation, most of which were up-regulated, as well as the down-regulation of genes encoding heat shock proteins and "microtubule-based movement" proteins. Our data provide novel information on the transcriptomic modulations in mussels facing temperature increases and high copper concentrations; these data highlight the risk of marine life exposed to toxic chemicals in the presence of temperature increases due to climate change. PMID:23825565

Negri, Alessandro; Oliveri, Catherina; Sforzini, Susanna; Mignione, Flavio; Viarengo, Aldo; Banni, Mohamed

2013-01-01

195

Effect of crude extract of Eugenia jambolana Lam. on human cytochrome P450 enzymes.  

PubMed

The fruit of Eugenia jambolana Lam. is very popular for its anti-diabetic property. Previous studies on the crude extract of E.?jambolana (EJE) have successfully explored the scientific basis for some of its traditional medicinal uses. Considering its wide use and consumption as a seasonal fruit, the present study investigates the ability of E.?jambolana to interact with cytochrome P450 enzymes. The standardized EJE was incubated with pooled human liver microsomes to assess the CYP2C9-, CYP2D6-, and CYP3A4-mediated metabolism of diclofenac, dextromethorphan, and testosterone, respectively. The metabolites formed after the enzymatic reactions were quantified by high performance liquid chromatography. EJE showed differential effect on cytochrome P450 activities with an order of inhibitory potential as CYP2C9?>?CYP3A4?>?CYP2D6 having IC50 of 76.69, 359.02, and 493.05?µg/mL, respectively. The selectivity of EJE for CYP2C9 rather than CYP3A4 and CYP2D6 led to perform the enzyme kinetics to explicate the mechanism underlying the inhibition of CYP2C9-mediated diclofenac 4'-hydroxylation. EJE was notably potent in inhibiting the reaction in a non-competitive manner with Ki of 84.85?±?5.27?µg/mL. The results revealed the CYP2C9 inhibitory potential of EJE with lower Ki value suggesting that EJE should be examined for its potential pharmacokinetic and pharmacodynamic interactions when concomitantly administered with other drugs. PMID:24590863

Chinni, Santhivardhan; Dubala, Anil; Kosaraju, Jayasankar; Khatwal, Rizwan Basha; Satish Kumar, M N; Kannan, Elango

2014-11-01

196

Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity  

PubMed Central

The present study was designed to investigate the antioxidant activity of aqueous and methanol extracts of Erythrina indica Lam leaves by in vitro methods viz. 1, 1-Diphenyl-2-Picrylhydrazyl, nitric oxide radical scavenging activity, and inhibition of lipid peroxidation by thiobarbituric acid reactive substances (TBARS) method on isolated rat liver tissues. Quantitative analysis of antioxidative components like total amount of phenolics, flavonoids, and flavonols were estimated using the spectrophotometric method. Linear regression analysis was used to calculate the IC50 value. Results showed that the aqueous and methanol extracts exhibited significant DPPH radicals scavenging activity with an IC50 value 342.59 ± 19.59, 283.24 ± 12.28 µg/mL respectively. Nitric oxide radicals were significantly scavenged by the aqueous and methanol extracts (IC50 = 250.12 ± 10.66; 328.29 ± 3.74 µg/mL). Lipid peroxidation induced by the Fe2+ was inhibited by the aqueous extract with low IC50 value (97.29 ± 2.05 µg/mL) as compared to methanol extract (IC50 = 283.74 ± 5.70 µg/mL). Both the extracts were exhibited similar quantities of total phenolics. Total flavonoids were found to be in higher quantities than total flavonols in aqueous extract as compared to methanol extract. From the results, it is concluded that the aqueous and methanol extracts of E. indica leaves possesses significant antioxidant activity that may be due to the presence of flavonoids and related polyphenolic compounds. PMID:21331194

Sakat, SS; Juvekar, AR

2010-01-01

197

Moringa oleifera Lam. (Moringaceae) grown in Nigeria: In vitro antisickling activity on deoxygenated erythrocyte cells  

PubMed Central

Context: Traditional medicine, which is more available and affordable for the poor uses medicinal plants for the treatment and management of various ailments, including the sickle cell disease (SCD). About 24 million Nigerians are carriers of this sickled cell gene, while approximately 2.4 million are SCD patients. Moringa oleifera Lam. (Moringaceae) possesses high nutritional value and has been used in folklore medicine to treat various ailments related to pain and inflammation. Chemical, pharmacological and pharmacognostical applications of Moringa oleifera have been reported. Objective: This study investigated the antisickling potential of polar and non-polar extracts of the seed, flower and leaf of Moringa oleifera for the first time. Materials and Methods: Using crude methanol extract, aqueous extract, ethyl acetate and butanol, the in vitro antisickling activities of Moringa oleifera fractions, were evaluated using erythrocyte cells deoxygenated with 2% sodium metabisulphite. p-Hydroxybenzoic acid and normal saline were employed as positive and negative controls. Results: Phytochemical screening revealed the presence of saponins, free anthraquinones, and alkaloids. Extracts of the seed and flower demonstrated a higher (P<0.05) antisickling activity in comparison to the leaf extract. The leaf extract, as well as those of the seed and flower, equally demonstrated a (P<0.05) reversal of sickled erythrocytes. Discussions and Conclusions: These findings suggest that Moringa oleifera may play a role in the management of SCD, by incorporation of its fractions into recipes. More extensive biological evaluations and further studies will be necessary for the chemical characterization of the antisickling principles. PMID:22557922

Adejumo, Olufunmilayo E.; Kolapo, Adelodun L.; Folarin, Akintomiwa O.

2012-01-01

198

In vitro antibacterial potency of Butea monosperma Lam. against 12 clinically isolated multidrug resistant bacteria  

PubMed Central

Objective To investigate the antibacterial activity, using cold and hot extraction procedures with five solvents, petroleum ether, acetone, ethanol, methanol and water to validate medicinal uses of Butea monosperma Lam (B. monosperma) in controlling infections; and to qualitatively estimate phytochemical constituents of leaf-extracts of the plant. Methods The antibacterial activity of leaf-extracts was evaluated by the agar-well diffusion method against clinically isolated 12 Gram-positive and -negative multidrug resistant (MDR) pathogenic bacteria in vitro. Values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of leaf-extracts against each bacterium were obtained in a 96-well micro-titre plate, by broth dilution micro-titre plate technique. Results The presence of tannins, flavonoids, starch, glycosides and carbohydrates in different leaf extracts was established. Pathogenic bacteria used were, Acinetobacter sp., Chromobacterium violaceum, Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Shigella sp., Enterococcus sp., Staphylococcus aureus (S. aureus), methicillin resistant S. aureus and vancomycin resistant S. aureus, along with standard bacterial strains. These MDR bacteria had been recorded to have significant inhibitions by leaf extracts, obtained by cold and hot extraction procedures with five solvents. In addition, the hot aqueous extract against Enterococcus sp. had the highest inhibition zone-size (21 mm). Ciprofloxacin 30 µg/disc was the positive/reference control and the diluting solvent, 10% dimethyl sulphoxide was the negative control. Recorded MIC values of different extracts ranged between 0.23 and 13.30 mg/mL, and MBC values were 0.52 to 30.00 mg/mL, for these bacteria. Conclusions Leaf-extracts with hot water and ethanol had shown significant antibacterial activity against all bacteria. B. monosperma leaf-extract could be used in treating infectious diseases, caused by the range of tested bacteria, as complementary and alternate medicine.

Sahu, Mahesh Chandra; Padhy, Rabindra Nath

2013-01-01

199

Anti-nutritional factors in the roots of a local cultivar of Moringa oleifera (Lam).  

PubMed

The evergreen plant, Moringa oleifera (Lam) has been known to have both medicinal and nutritional properties, thus its wide use in traditional medicine in Africa and Asia. The roots, in particular, have been reported to possess antibiotic, anti-tumour and anti-oxidative activities. This study therefore seeks to determine the levels of the anti-nutritional factors and other proximate analyses in the roots of a local cultivar of Moringa oleifera which might be responsible for such activities. The concentrations of oxalates and phytates in the roots were determined using the methods of Munro and Bassir and Griffith and Thomas, respectively. The methods of Association of Analytical Chemists (AOAC) were used to estimate the amount of tannins, saponins and cyanogenic glycosides while the Technicon sequential Multi-sample amino acid analyzer (TSM) was used determine the amino acid concentration in the roots. Tannins (45 mg 100 g(-1)) and oxalates (17.08 mg 100 g(-1)) were present in the roots at higher levels while saponins (4.20mg 100 g(-1)), cyanogenic glycosides (2.72 mg 100 g(-1)) and phytates (0.07 mg 100 g(-1)) occurred at much lower levels. The roots contained (Mean +/- Standard Error of mean) %crude lipid (6.33 +/- 1.64), %crude proteins (5.02 +/- 1.52), %carbohydrates (76.75), %ash (4.97 +/- 0.53) and %moisture (6.93 +/- 0.58). The roots lacked the water-soluble vitamins pyridoxine, riboflavin and thiamine but contained ascorbic acid (48.13 mg 100 g(-1)) and niacin (5.83 mg 100 g(-1)). This study has shown that Moringa oleifera roots are rich in anti-nutritional factors and that is why they are widely used in traditional medicine in Africa, Asia and Americas for its medicinal importance. PMID:24783788

Igwilo, I O; Ezeonu, F C; Ezekwesili-Ofili, J O; Igwilo, S N; Nsofor, C I; Abdulsalami, M S; Obi, E

2014-01-01

200

Chemical characteristics and fractionation of proteins from Moringa oleifera Lam. leaves.  

PubMed

Moringa oleifera Lam. is a leguminous plant, originally from Asia, which is cultivated in Brazil because of its low production cost. Although some people have used this plant as food, there is little information about its chemical and nutritional characteristics. The objective of this study was to characterise the leaves of M. oleifera in terms of their chemical composition, protein fractions obtained by solubility in different systems and also to assess their nutritional quality and presence of bioactive substances. The whole leaf flour contained 28.7% crude protein, 7.1% fat, 10.9% ashes, 44.4% carbohydrate and 3.0mg 100g(-1) calcium and 103.1mg 100g(-1) iron. The protein profile revealed levels of 3.1% albumin, 0.3% globulins, 2.2% prolamin, 3.5% glutelin and 70.1% insoluble proteins. The hydrolysis of the protein from leaf flour employing sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (ME) resulted in 39.5% and 29.5%, respectively. The total protein showed low in vitro digestibility (31.8%). The antinutritional substances tested were tannins (20.7 mg g(-1)), trypsin inhibitor (1.45TIU mg g(-1)), nitrate (17 mg g(-1)) and oxalic acid (10.5 mg g(-1)), besides the absence of cyanogenic compounds. ?-Carotene and lutein stood out as major carotenoids, with concentrations of 161.0 and 47.0 ?g g(-1) leaf, respectively. Although M. oleifera leaves contain considerable amount of crude protein, this is mostly insoluble and has low in vitro digestibility, even after heat treatment and chemical attack. In vivo studies are needed to better assess the use of this leaf as a protein source in human feed. PMID:24206684

Teixeira, Estelamar Maria Borges; Carvalho, Maria Regina Barbieri; Neves, Valdir Augusto; Silva, Maraíza Apareci; Arantes-Pereira, Lucas

2014-03-15

201

Transcriptional Response of the Mussel Mytilus galloprovincialis (Lam.) following Exposure to Heat Stress and Copper  

PubMed Central

Global warming is a major factor that may affect biological organization, especially in marine ecosystems and in coastal areas that are particularly subject to anthropogenic pollution. We evaluated the effects of simultaneous changes in temperature and copper concentrations on lysosomal membrane stability (N-acetyl-hexosaminidase activity) and malondialdehyde accumulation (MDA) in the gill of the blue mussel Mytilus galloprovincialis (Lam.). Temperature and copper exerted additive effects on lysosomal membrane stability, exacerbating the toxic effects of metal cations present in non-physiological concentrations. Mussel lysosomal membrane stability is known to be positively related to scope for growth, indicating possible effects of increasing temperature on mussel populations in metal-polluted areas. To clarify the molecular response to environmental stressors, we used a cDNA microarray with 1,673 sequences to measure the relative transcript abundances in the gills of mussels exposed to copper (40 µg/L) and a temperature gradient (16°C, 20°C, and 24°C). In animals exposed only to heat stress, hierarchical clustering of the microarray data revealed three main clusters, which were largely dominated by down-regulation of translation-related differentially expressed genes, drastic up-regulation of protein folding related genes, and genes involved in chitin metabolism. The response of mussels exposed to copper at 24°C was characterized by an opposite pattern of the genes involved in translation, most of which were up-regulated, as well as the down-regulation of genes encoding heat shock proteins and “microtubule-based movement” proteins. Our data provide novel information on the transcriptomic modulations in mussels facing temperature increases and high copper concentrations; these data highlight the risk of marine life exposed to toxic chemicals in the presence of temperature increases due to climate change. PMID:23825565

Negri, Alessandro; Oliveri, Catherina; Sforzini, Susanna; Mignione, Flavio; Viarengo, Aldo; Banni, Mohamed

2013-01-01

202

Frequency and intensity patterns of LAM progression in weakly coupled chains: The case of stearic acid and stearyl alcohol  

NASA Astrophysics Data System (ADS)

The coupled oscillator model previously described for the interpretation of longitudinal acoustical modes (LAM) of weakly coupled chain molecules has been extended in order to account for the observed frequency and intensity patterns in stearic acid and stearyl alcohol. The model and its general validity are discussed and a specific comparison is made with data obtained from Raman measurements on hexatriacontane (n-C36H74), stearic acid (n-C17H35-COOH), and stearyl alcohol (n-C18H37OH).

Minoni, Giuseppe; Zerbi, Giuseppe; Rabolt, J. F.

1984-12-01

203

Clinical Utility of a Commercial LAM-ELISA Assay for TB Diagnosis in HIV-Infected Patients Using Urine and Sputum Samples  

PubMed Central

Background The accurate diagnosis of TB in HIV-infected patients, particularly with advanced immunosuppression, is difficult. Recent studies indicate that a lipoarabinomannan (LAM) assay (Clearview-TB®-ELISA) may have some utility for the diagnosis of TB in HIV-infected patients; however, the precise subgroup that may benefit from this technology requires clarification. The utility of LAM in sputum samples has, hitherto, not been evaluated. Methods LAM was measured in sputum and urine samples obtained from 500 consecutively recruited ambulant patients, with suspected TB, from 2 primary care clinics in South Africa. Culture positivity for M. tuberculosis was used as the reference standard for TB diagnosis. Results Of 440 evaluable patients 120/387 (31%) were HIV-infected. Urine-LAM positivity was associated with HIV positivity (p?=?0.007) and test sensitivity, although low, was significantly higher in HIV-infected compared to uninfected patients (21% versus 6%; p<0.001), and also in HIV-infected participants with a CD4 <200 versus >200 cells/mm3 (37% versus 0%; p?=?0.003). Urine-LAM remained highly specific in all 3 subgroups (95%–100%). 25% of smear-negative but culture-positive HIV-infected patients with a CD4 <200 cells/mm3 were positive for urine-LAM. Sputum-LAM had good sensitivity (86%) but poor specificity (15%) likely due to test cross-reactivity with several mouth-residing organisms including actinomycetes and nocardia species. Conclusions These preliminary data indicate that in a high burden primary care setting the diagnostic usefulness of urine-LAM is limited, as a rule-in test, to a specific patient subgroup i.e. smear-negative HIV-infected TB patients with a CD4 count <200 cells/mm3, who would otherwise have required further investigation. However, even in this group sensitivity was modest. Future and adequately powered studies in a primary care setting should now specifically target patients with suspected TB who have advanced HIV infection. PMID:20352098

Dheda, Keertan; Davids, Virginia; Lenders, Laura; Roberts, Teri; Meldau, Richard; Ling, Daphne; Brunet, Laurence; van Zyl Smit, Richard; Peter, Jonathan; Green, Clare; Badri, Motasim; Sechi, Leonardo; Sharma, Surendra; Hoelscher, Michael; Dawson, Rodney; Whitelaw, Andrew; Blackburn, Jonathan; Pai, Madhukar; Zumla, Alimuddin

2010-01-01

204

In vivo anticoccidial activity of berberine [18, 5,6-dihydro-9,10-dimethoxybenzo(g)-1,3-benzodioxolo(5,6-a) quinolizinium]--an isoquinoline alkaloid present in the root bark of Berberis lycium.  

PubMed

Coccidiosis, caused by various Eimeria species, is a major parasitic disease in chicken. However the increasing resistance of these parasites to currently used anticoccidial drugs has stimulated the search for new methods of control. As part of this effort we investigated the root bark of Berberis lycium (barberry) as a potential source of compounds with anticoccidial activity. In the present study anticoccidial activity of different solvent extracts of the root bark of B. lycium and berberine was evaluated in vivo using broiler chicken. Results of the study demonstrated equipotent efficacy of pure berberine in comparison to that of standard drug amprolium on the basis of reduction in coccidian oocyst output, body weight gain of chicken and feed conversion ratio. Among the extracts crude methanolic extract showed highest anticoccidial activity tested at 300 mg/kg body weight which could be due to the presence of alcohol-soluble active ingredients in root bark of B. lycium. Toxicological studies revealed that B. lycium extracts as well as berberine were not lethal up to dosage of 2000 mg/kg body weight. LD(50) was not determined as mortalities were not recorded in any of the five groups of chicken. From the present study it can be concluded that root bark of B. lycium has the immense potential to contribute to the control of coccidian parasites of chicken. Our results corroborate the use of berberine for treatment of severe diarrhoea, amoebiasis and intestinal infections and could justify its use in folk medicine for treatment of haemorrhagic dysentery. PMID:24411651

Malik, Tauseef Ahmad; Kamili, Azra N; Chishti, M Z; Tanveer, Syed; Ahad, Shazia; Johri, R K

2014-04-15

205

Atividade antioxidante e captora de radicais livres dos extratos de Achyrocline alata (Kunth.) DC. em comparação com extratos de Achyrocline satureioides (Lam.) DC  

Microsoft Academic Search

RESUMO: Achyrocline alata (Kunth.) DC. e Achyrocline satureioides (Lam.) DC., espécies pertencentes à família Asteraceae, são utilizadas na medicina popular de Campo Grande, Mato Grosso do Sul, como plantas sucedâneas. O presente trabalho teve com objetivo comparar os extratos obtidos dessas espécies através de ensaios químicos simples destinados a testar as atividades antioxidante e captora de radicais livres, utilizando como

Rafaela F. Grassi-Zampieron; Maria C. Vieira; João M. de Siqueira

2009-01-01

206

Patrick Lam Patrick received his Ph.D. from the University of Rochester (Dr. Louis Friedrich) and was a postdoctoral fellow in UCLA (Prof. Mike Jung and  

E-print Network

-discoverer of the powerful Chan-Lam Coupling Reaction. Patrick has worked with CROs successfully and productively for ten/patent applications. Discovery of Eliquis®/Apixaban, a novel Factor Xa inhibitor as a transformational new in patients with nonvalvular atrial fibrillation. Eliquis® was recently named the "Best New Medicine of 2012

Tian, Weidong

207

The ?-Glucanase ZgLamA from Zobellia galactanivorans Evolved a Bent Active Site Adapted for Efficient Degradation of Algal Laminarin*  

PubMed Central

Laminarinase is commonly used to describe ?-1,3-glucanases widespread throughout Archaea, bacteria, and several eukaryotic lineages. Some ?-1,3-glucanases have already been structurally and biochemically characterized, but very few from organisms that are in contact with genuine laminarin, the storage polysaccharide of brown algae. Here we report the heterologous expression and subsequent biochemical and structural characterization of ZgLamAGH16 from Zobellia galactanivorans, the first GH16 laminarinase from a marine bacterium associated with seaweeds. ZgLamAGH16 contains a unique additional loop, compared with other GH16 laminarinases, which is composed of 17 amino acids and gives a bent shape to the active site cleft of the enzyme. This particular topology is perfectly adapted to the U-shaped conformation of laminarin chains in solution and thus explains the predominant specificity of ZgLamAGH16 for this substrate. The three-dimensional structure of the enzyme and two enzyme-substrate complexes, one with laminaritetraose and the other with a trisaccharide of 1,3–1,4-?-d-glucan, have been determined at 1.5, 1.35, and 1.13 ? resolution, respectively. The structural comparison of substrate recognition pattern between these complexes allows the proposition that ZgLamAGH16 likely diverged from an ancestral broad specificity GH16 ?-glucanase and evolved toward a bent active site topology adapted to efficient degradation of algal laminarin. PMID:24337571

Labourel, Aurore; Jam, Murielle; Jeudy, Alexandra; Hehemann, Jan-Hendrik; Czjzek, Mirjam; Michel, Gurvan

2014-01-01

208

Abstract --This paper presents an approach for modeling and model-checking a mobile agent system specified by LAM, which is a two-layer  

E-print Network

Abstract -- This paper presents an approach for modeling and model- checking a mobile agent system specified by LAM, which is a two-layer formal method for characterizing logical agent mobility using checker SPIN, we model check a variety of properties with respect to agents, logical agent mobility, agent

209

Genetic Diversity of the Greater Yam ( Dioscorea alata L.) and Relatedness to D. nummularia Lam. and D. transversa Br. as Revealed with AFLP Markers  

Microsoft Academic Search

Amplified fragment length polymorphism markers were used to assess the genetic relatedness between Dioscorea alata and nine other edible Dioscorea. These species include D. abyssinica Hoch., D. bulbifera L., D. cayenensis-rotundata Lamk. et Poir., D. esculenta Burk., D. nummularia Lam., D. pentaphylla L., D. persimilis Prain. et Burk., D. transversa Br. and D. trifida L. Four successive studies were conducted

R. Malapa; G. Arnau; J. L. Noyer; V. Lebot

2005-01-01

210

Determination of infochemicals and the phytochemical screening of the foliage and stem-bark of Senna siamea (lam.) in Yola, Adamawa State  

Microsoft Academic Search

Alkaloids, anthraquinonnes, cardiac glycosides, flavonoids, phlobatannin, polyphenols, saponins, steroids, tannins and terpenoids were ten groups of infochemicals studied in the foliage and stem-bark of Senna siamea (Lam.), a plantation species grown in Yola. The experimental design was the Randomized Complete Block Design (RCBD) with four replications. Results obtained from the experiments indicated that all the ten groups of infochemicals were

Ayuba Daniel Kwada; I. O. Tell

211

Perera Lam: An environmental justice assessment of the Mississippi River Industrial Corridor in Louisiana, U.S. APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 11(4): 681-697.  

E-print Network

Perera ­ Lam: An environmental justice assessment of the Mississippi River Industrial Corridor JUSTICE ASSESSMENT OF THE MISSISSIPPI RIVER INDUSTRIAL CORRIDOR IN LOUISIANA, U.S. USING A GIS to assess the status of environmental justice concerns in the Mississippi River Industrial Corridor

212

The Learning Affect Monitor (LAM): A Computer-Based System Integrating Dimensional and Discrete Assessment of Affective States in Daily Life  

Microsoft Academic Search

The Learning Affect Monitor (LAM) is a new computer-based assessment system integrating basic dimensional evaluation and discrete description of affective states in daily life, based on an autonomous adapting system. Subjects evaluate their affective states according to a tridimensional space (valence and activation circumplex as well as global intensity) and then qualify it using up to 30 adjective descriptors chosen

Michael Reicherts; Virginie Salamin; Christian Maggiori; Karl Pauls

2007-01-01

213

Biochemical and physiological alterations in female reproductive organs of cyclic rats treated with aqueous extract of Moringa oleifera Lam.  

PubMed

Biochemical and physiological alterations have been observed in the genital tract of female cyclic rats treated with aqueous extract of M. oleifera Lam. Its administration caused a significant increase in the glycogen contents, protein concentration, activity of acid and alkaline phosphatase and the level of total cholesterol in all the organs at initial days of treatment. However, at longer days of treatment the values revealed a significant depletion. Initially its administration stimulated the uterine structures, caused metaplastic changes in the cervical epithelium and provoked considerable cornification in the vaginal epithelium. At later durations significant inhibition in the histoarchitecture was observed. Biochemical observations supplemented with the histological findings have been correlated with the anti-implantation action of the aqueous extract in the light of its hormonal properties. PMID:3227756

Shukla, S; Mathur, R; Prakash, A O

1988-01-01

214

Studies of Pb2+ adsorption by Moringa oleifera Lam. seeds from an aqueous medium in a batch system.  

PubMed

The efficiency of Moringa (Moringa oleifera Lam.) seeds for removing lead ions (Pb(2+)) from water was evaluated. Parameters such as solution pH, adsorbent mass, contact time between solution and adsorbent, isotherms, thermodynamic, kinetics, and desorption were evaluated. The maximum adsorption capacity of the biosorbent was found to be 12.24 mg g(-1). In order to verify the effectiveness of this material, comparative studies were performed with activated carbon under the same optimal conditions for the construction of isotherms and the desorption process. Average desorption rate values led to the assumption that a strong interaction took place between the adsorbents and the metal ions. Thus, it has been concluded that the biosorbent studied herein can be considered very effective and feasible for remediating Pb(2+)-contaminated solutions, since this material is itself an untreated and low-cost byproduct. PMID:24434983

Meneghel, Ana Paula; Gonçalves, Affonso Celso; Tarley, César Ricardo Teixeira; Stangarlin, José Renato; Rubio, Fernanda; Nacke, Herbert

2014-01-01

215

Bioactive secondary metabolites from Nigrospora sp. LLGLM003, an endophytic fungus of the medicinal plant Moringa oleifera Lam.  

PubMed

An endophytic fungus was isolated from the root of the medicinal plant Moringa oleifera Lam. Based on analyzing the rDNA sequence, the fungus was identified as Nigrospora sp. This is the first report of the isolation of endophytic Nigrospora from M. oleifera. By bioassay-guided fractionation, four antifungal secondary metabolites were isolated from liquid cultures of the fungus Nigrospora sp. LLGLM003, and their chemical structures were determined to be griseofulvin (1), dechlorogriseofulvin (2), 8-dihydroramulosin (3) and mellein (4) on the basis of spectroscopic analyses. Compound 2, 3 and 4 were isolated from Nigrospora sp. for the first time. In vitro antifungal assay showed that griseofulvin displayed clear inhibition of the growth of 8 plant pathogenic fungi. Dechlorogriseofulvin and mellein exhibited only weak antifungal activities, whereas 8-dihydroramulosin displayed no antifungal activities. PMID:22806033

Zhao, J H; Zhang, Y L; Wang, L W; Wang, J Y; Zhang, C L

2012-05-01

216

The Effects of Berberis vulgaris Fruit Extract on Serum Lipoproteins, apoB, apoA-I, Homocysteine, Glycemic Control and Total Antioxidant Capacity in Type 2 Diabetic Patients.  

PubMed

Type 2 diabetes is a well-known endocrine and metabolic disorder which has reached epidemic proportions worldwide and represents a serious public health concern. Hyperglycemia and dyslipidemia are two major abnormalities which are major cardiovascular risk factors. Berberine is a major alkaloid in Berberis vulgaris fruit extract (BVFE) which have important role in regulation of serum glucose and fat metabolism in-vivo and in-vitro but its role in type 2 diabetes have not been extensively examined. The aim of this study was the effect of BVFE on serum lipoproteins, apoB, apoA-I, homocysteine, glycemic control and total antioxidant capacity in type 2 diabetic patients. In a double-blind randomised clinical trial, 31 diabetic patients were randomly assigned to 3 g/d BVFE or placebo for 3 months. Serum glucose, lipoproteins, apoB, apoA-I, insulin, homocysteine and HbA1c were measured at the baseline and also at the end of the 3(rd) month. At the beginning and end of 1(st), 2(nd) and 3(rd) months, a 24-h dietary recall questionnaire about each patients was completed. Data were analyzed by SPSS version 16. There were significant decreases in serum TG, TC, LDL-c, apo B, glucose, and insulin and also a significant increase in TAC at the end of the study in BVFE group compared to the control group (p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.002, p = 0.01 and p = 0.0001 respectively). There were significant differences in serum TG (p = 0.0001), TC (p = 0.001), LDL-c (p = 0.001), apoB (p = 0.001), glucose (p = 0.002), insulin (p = 0.01), TAC (p = 0.005), and insulin resistance (p = 0.01) between the two groups at the end of the study; but homocysteine, HbA1c and HDL-c showed no significant changes between the two groups at the end of study. The intake of 3 g/d BVFE for 3 months may have benefical effects on lipoproteins, apoproteins, glycemic control and TAC in type 2 diabetic patients. PMID:24250489

Shidfar, Farzad; Ebrahimi, Shima Seyyed; Hosseini, Sharieh; Heydari, Iraj; Shidfar, Shahrzad; Hajhassani, Giti

2012-01-01

217

The Effects of Berberis vulgaris Fruit Extract on Serum Lipoproteins, apoB, apoA-I, Homocysteine, Glycemic Control and Total Antioxidant Capacity in Type 2 Diabetic Patients  

PubMed Central

Type 2 diabetes is a well-known endocrine and metabolic disorder which has reached epidemic proportions worldwide and represents a serious public health concern. Hyperglycemia and dyslipidemia are two major abnormalities which are major cardiovascular risk factors. Berberine is a major alkaloid in Berberis vulgaris fruit extract (BVFE) which have important role in regulation of serum glucose and fat metabolism in-vivo and in-vitro but its role in type 2 diabetes have not been extensively examined. The aim of this study was the effect of BVFE on serum lipoproteins, apoB, apoA-I, homocysteine, glycemic control and total antioxidant capacity in type 2 diabetic patients. In a double-blind randomised clinical trial, 31 diabetic patients were randomly assigned to 3 g/d BVFE or placebo for 3 months. Serum glucose, lipoproteins, apoB, apoA-I, insulin, homocysteine and HbA1c were measured at the baseline and also at the end of the 3rd month. At the beginning and end of 1st, 2nd and 3rd months, a 24-h dietary recall questionnaire about each patients was completed. Data were analyzed by SPSS version 16. There were significant decreases in serum TG, TC, LDL-c, apo B, glucose, and insulin and also a significant increase in TAC at the end of the study in BVFE group compared to the control group (p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.002, p = 0.01 and p = 0.0001 respectively). There were significant differences in serum TG (p = 0.0001), TC (p = 0.001), LDL-c (p = 0.001), apoB (p = 0.001), glucose (p = 0.002), insulin (p = 0.01), TAC (p = 0.005), and insulin resistance (p = 0.01) between the two groups at the end of the study; but homocysteine, HbA1c and HDL-c showed no significant changes between the two groups at the end of study. The intake of 3 g/d BVFE for 3 months may have benefical effects on lipoproteins, apoproteins, glycemic control and TAC in type 2 diabetic patients. PMID:24250489

Shidfar, Farzad; Ebrahimi, Shima Seyyed; Hosseini, Sharieh; Heydari, Iraj; Shidfar, Shahrzad; Hajhassani, Giti

2012-01-01

218

Suppressive effects of Moringa oleifera Lam pod against mouse colon carcinogenesis induced by azoxymethane and dextran sodium sulfate.  

PubMed

Moringa oleifera Lam (horseradish tree; tender pod or fruits) is a major ingredient in Thai cuisine and has some medicinal properties. Previous studies have shown potentially antioxidant, antitumor promoter, anticlastogen and anticarcinogen activities both in vitro and in vivo. The present study was conducted to investigate chemopreventive effects on azoxymethane (AOM)-initiated and dextran sodium sulfate (DSS)-promoted colon carcinogenesis in mice. Male ICR mice were divided into 8 groups: Group 1 served as a negative control; Group 2 received AOM/DSS as a positive control; Groups 3-5 were fed boiled freeze-dried M. oleifera (bMO) at 1.5%, 3.0% and 6.0%, respectively supplemented in basal diets for 5 weeks; Groups 6-8 were fed with bMO diets at the designed doses above for 2 weeks prior to AOM, during and 1 week after DSS administration. At the end of the study, colon samples were processed for histopathological examination. PCNA indices, and iNOS and COX-2 expression were assessed by immunohistochemistry. The results demonstrated the incidences and multiplicities of tumors in Groups 6-8 to be decreased when compared to Group 2 in a dose dependent manner, but this was significant only in Group 8. The PCNA index was also significantly decreased in Group 8 whereas iNOS and COX-2 protein expression were significantly decreased in Groups 7 and 8. The findings suggest that M. oleifera Lam pod exerts suppressive effects in a colitis-related colon carcinogenesis model induced by AOM/DSS and could serve as a chemopreventive agent. PMID:22471457

Budda, Sirintip; Butryee, Chaniphun; Tuntipopipat, Siriporn; Rungsipipat, Anudep; Wangnaithum, Supradit; Lee, Jeong-Sang; Kupradinun, Piengchai

2011-01-01

219

Production of in vitro haploid plants from in situ induced haploid embryos in winter squash ( Cucurbita maxima Duchesne ex Lam.) via irradiated pollen  

Microsoft Academic Search

The influence of pollen irradiation on the production of in vitro haploid plants from in situ induced haploid embryos was\\u000a investigated in winter squash (Cucurbita maxima Duchesne ex Lam.). Pollen were irradiated at different gamma-ray doses (50, 100, 200 and 300 Gray) and durations (9, 11,\\u000a 15, 21, and 28 July). Production of in vitro haploid plantlets was influenced by

Ertan Sait Kurtar; Ahmet Balkaya

2010-01-01

220

Inhibitory Effect of n-butanol Fraction of Moringa oleifera Lam. Seeds on Ovalbumin-Induced Airway Inflammation in a Guinea Pig Model of Asthma  

Microsoft Academic Search

Moringaceae, which belongs to the Moringa oleifera Lam. family, is a well-known herb used in Asian medicine as an antiallergic drug. In the present study, the efficacy of the n-butanol extract of the seeds of the plant (MONB) is examined against ovalbumin-induced airway inflammation in guinea pigs. The test drugs (MONB or dexamethasone) are administered orally prior to challenge with

Shailaja G. Mahajan; Aryamitra Banerjee; Bhupendrasinh F. Chauhan; Harish Padh; Manish Nivsarkar; Anita A. Mehta

2009-01-01

221

Corchorusin-D, a saikosaponin-like compound isolated from Corchorus acutangulus Lam., targets mitochondrial apoptotic pathways in leukemic cell lines (HL60 and U937)  

Microsoft Academic Search

Purpose  The presence of triterpene saponins in Corchorus acutangulus Lam. has been reported. However, no studies concerning biological activity of the plant extracts have been done so far. In\\u000a the present study, the anti-leukemic activity of the methanol extract of aerial parts (ME) of C. acutangulus has been investigated, and efforts have been made to identify the active ingredient responsible for

Sumana Mallick; Papiya Ghosh; Suman K. Samanta; Sumita Kinra; Bikas C. Pal; Aparna Gomes; Joseph R. Vedasiromoni

2010-01-01

222

Preface: Proceedings of the 13th Conference on Liquid and Amorphous Metals (LAM13) (Ekaterinburg, Russia, 8 14 July 2007)  

NASA Astrophysics Data System (ADS)

The most recent developments in the field of liquid and amorphous metals and alloys are regularly updated through two complementary international conferences: the liquid and amorphous metals conference (LAM) and the rapidly quenched materials (RQ) conference. The first series of conferences started as LM1 in 1966 at Brookhaven for the basic understanding of liquid metals. The subsequent LM conferences were held in Tokyo (1972) and Bristol (1976). The conference was renewed in Grenoble (1980) as a LAM conference including amorphous metals and continued in Los Angeles (1983), Garmisch-Partenkirchen (1986), Kyoto (1989), Vienna (1992), Chicago (1995), Dortmund (1998), Yokohama (2001) and Metz (2004). The conferences are mainly devoted to liquid and amorphous metals and alloys. However, communications on some non-metallic systems such as semiconductors, quasicrystals etc, are also accepted. The conference tradition strongly encourages participation from junior researchers and graduate students. The 13th conference of the LAM series was organized in Ekaterinburg, Russia, by the Institute of Metallurgy of the Ural Branch of the Russian Academy of Sciences (IMet UB RAS) and the Ural State Pedagogical University (USPU), and held from 8-14 July 2007 under the chairmanship of Professors Pjotr Popel (USPU) and Boris Gelchinskii (IMet UB RAS). Two hundred and forty two active participants and about 60 guest participants from 20 countries attended the conference. There were no parallel sessions and all oral reports were separated into three groups: invited talks (40 min), full-scale oral reports (25 min), and brief oral reports (15 min). The program included ten sessions, ranging from purely theoretical subjects to the technological application of molten and amorphous alloys. The following sessions took place: A: Electronic structure and transport, magnetic properties; B: Phase transitions; C: Structure; D: Atomic dynamics and transport; E: Thermodynamics; F: Modelling, simulation; G: Surface and interface; H: Mechanical properties and new materials; I: Quasicrystals; J: Industrial applications. The largest sessions were E, C, D and F with 53, 47, 43 and 40 reports, respectively. Posters were exhibited during the first (sessions A-E) or last (sessions F-J) three days of the conference. As usual, each oral session started with an invited talk. In accordance with contemporary tendencies the first invited talk of M Yao (Kyoto University) was devoted to interrelations between liquid metal studies and nanoscience. He pointed out that the physics of disordered matter, especially liquid metals, has provided nanoscience with basic ideas and theoretical tools such as ab initio molecular dynamics (MD) simulation. K Tamura (Kyoto University) reported experimental investigation results of expanded liquid rubidium, showing that observed structural features are originated from the instability of low-density electron gas. Modern theory has predicted that interacting electron gas suffers negative compressibility and the static dielectric function (DF) becomes negative when electron density is sufficiently reduced. Negative DF is of special interest when considering the possibility of new types of superconductors. In the next invited lecture, M I Mendelev and J R Morris (USA) described how MD simulations were performed to study phase transformations in supercooled liquid Al. The authors found that the widely used EAM potential for Al enables vitrification for cooling rates achievable in classical MD simulation, while other Al potentials enable crystallization under the same conditions. The reason for this difference was discussed, as well as features of vitrification in the EA Al. P Häussler (Chemnitz Univeristy) spoke about fundamental structure-forming processes in liquid and amorphous materials. He proposed the resonance model as an autonomous missing link between the microscopic description of atoms/molecules of Schrödinger's equation, and crystals, where global concepts such as planar resonances exist and Bloch's theorem is applied. D Holland-

Popel, Pjotr; Gelchinskii, Boris; Sidorov, Valeriy

2008-03-01

223

Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis  

PubMed Central

Objectives Moxifloxacin (MXF) has been advocated for the treatment of extensively drug-resistant (XDR) tuberculosis despite resistance to older-generation fluoroquinolones. We investigated the relationship between the minimum inhibitory concentration (MIC) of MXF and mutations in the gyrA and gyrB genes in Mycobacterium tuberculosis (MTB) isolates from KwaZulu-Natal (KZN) Province of South Africa. Materials and methods MICs of 56 MTB isolates were compared to the mutations in the quinolone resistance-determining region known to confer fluoroquinolone resistance. Isolates were genotyped by IS6110 restriction fragment length polymorphism analysis. Results The circulating F15/LAM4/KZN XDR strain circulating in KZN Province harbored the A90V mutation and displayed high-level resistance with MICs of 8 mg/L for ciprofloxacin and ofloxacin and ?1 mg/L for MXF. Conclusion The inclusion of MXF in XDR-TB treatment regimens requires careful consideration in our setting, where clinical outcome data in MXF-containing regimens are unavailable. PMID:25187730

Dookie, Navisha; Sturm, A Willem; Moodley, Prashini

2014-01-01

224

(±)-catechin, a root exudate of the invasive centaurea stoebe lam. (Spotted knapweed) exhibits bacteriostatic activity against multiple soil bacterial populations.  

PubMed

Understanding the effects of allelopathic plant chemicals on soil microorganisms is critical to understanding their ecological roles and importance in exotic plant invasion. Centaurea stoebe Lam. (spotted knapweed), an aggressive invasive weed in North America, secretes a racemic mixture of (±)-catechin as a root exudate. This enantiomeric, polyphenolic compound has been reported to have allelopathic effects on surrounding flora and microflora. To better understand how catechin affects microbial communities in the root zone of spotted knapweed, we assessed its impact on the total culturable bacterial component and numerous individual bacterial populations from Romanian (native range) and Montana (invaded range) soils. Catechin suppressed total culturable count numbers from the bacterial community and inhibited growth of some, but not all, soil bacterial populations tested. The native soil bacterial community was significantly more resistant to inhibitory effects of catechin than either the invaded or non-invaded soils. We further show that the inhibitory effect of catechin on nine different soil bacterial strains from seven genera was reversible, demonstrating that it acts via a bacteriostatic rather than bactericidal mechanism. These findings suggest that catechin might affect bacterial community composition and activity in the root zone. PMID:21882071

Pollock, Jarrod L; Kogan, Lewis A; Thorpe, Andrea S; Holben, William E

2011-09-01

225

Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes.  

PubMed

Toddalia asiatica (L.) Lam. (T. asiatica) has been utilized traditionally for medicinal purposes such as the treatment of diabetes. Currently, the extract is considered to be a good source of anti-diabetic agents, but the active compounds have yet to be identified. In this study, we investigated the effects of fractionated T. asiatica extracts on the differentiation of 3T3-L1 preadipocytes and identified aculeatin as a potential active agent. When 3T3-L1 preadipocytes were treated with aculeatin isolated from T. asiatica in the presence of insulin, aculeatin increased cellular triglyceride levels and glycerol-3-phosphate dehydrogenase activity. This indicated that aculeatin could enhance the differentiation of preadipocytes into adipocytes. Further analyses using a DNA microarray and real-time quantitative reverse-transcription PCR showed an increase in the expression of peroxisome proliferator-activated receptor-? target genes (Pparg, Ap2, Cd36, Glut4 and Adipoq) by aculeatin, suggesting that aculeatin enhances the differentiation of 3T3-L1 cells by modulating the expression of genes critical for adipogenesis. Interestingly, after treatment of differentiated adipocytes with aculeatin, glucose uptake and lipolysis were enhanced. Overall, our results suggested that aculeatin is an active compound in T. asiatica for enhancing both differentiation and lipolysis of adipocytes, which are useful for the treatment of lipid abnormalities as well as diabetes. PMID:25445590

Watanabe, Akio; Kato, Tsuyoshi; Ito, Yusuke; Yoshida, Izumi; Harada, Teppei; Mishima, Takashi; Fujita, Kazuhiro; Watai, Masatoshi; Nakagawa, Kiyotaka; Miyazawa, Teruo

2014-10-31

226

An Ethyl Acetate Fraction of Moringa oleifera Lam. Inhibits Human Macrophage Cytokine Production Induced by Cigarette Smoke  

PubMed Central

Moringa oleifera Lam. (MO) has been reported to harbor anti-oxidation and anti-inflammatory activity and useful in the treatment of inflammatory diseases. However, despite these findings there has been little work done on the effects of MO on immune cellular function. Since macrophages, TNF and related cytokines play an important pathophysiologic role in lung damage induced by cigarette smoke, we examined the effects of MO on cigarette smoke extract (CSE)—induced cytokine production by human macrophages. An ethyl acetate fraction of MO (MOEF) was prepared from fresh leaves extract of Moringa and shown to consist of high levels of phenolic and antioxidant activities. Human monocyte derived macrophages (MDM) pre-treated with varying concentrations of MOEF showed decreased production of TNF, IL-6 and IL-8 in response to both LPS and CSE. The decrease was evident at both cytokine protein and mRNA levels. Furthermore, the extract inhibited the expression of RelA, a gene implicated in the NF-?B p65 signaling in inflammation. The findings highlight the ability of MOEF to inhibit cytokines (IL-8) which promote the infiltration of neutrophils into the lungs and others (TNF, IL-6) which mediate tissue disease and damage. PMID:24553063

Kooltheat, Nateelak; Pankla Sranujit, Rungnapa; Chumark, Pilaipark; Potup, Pachuen; Laytragoon-Lewin, Nongnit; Usuwanthim, Kanchana

2014-01-01

227

Antinociceptive effect of an ethanolic extract of the aerial parts of Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae)  

PubMed Central

Background: Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae) is a perennial herb used in Ghanaian traditional medicine for the treatment of various painful conditions. Little scientific evidence exists in literature on the effect of this plant on pain. Materials and Methods: The present study examined the antinociceptive effect of the ethanolic extract of the aerial parts of H. latifolia in chemical (acetic acid-induced abdominal writhing, glutamate, formalin, and capsaicin tests) and thermal (tail immersion test) behavioral pain models in rodents. The possible mechanisms of the antinociceptive action were also assessed with various antagonists in the formalin test. Results: The H. latifolia extract (HLE) together with morphine and diclofenac (positive controls), showed significant antinociceptive activity in all the models used. The antinociceptive effect exhibited by HLE in the formalin test was partly or wholly reversed by the systemic administration of naloxone, theophylline, and atropine. Glibenclamide, ondansetron, yohimbine, nifedipine, and NG-L-nitro-arginine methyl ester (L-NAME), however, did not significantly block the antinociceptive effect of the extract. HLE, unlike morphine, did not induce tolerance to its antinociceptive effect in the formalin test after chronic administration; morphine tolerance did not also cross-generalize to HLE. Interestingly, also, the chronic concomitant administration of HLE and morphine significantly suppressed the development of morphine tolerance. Conclusion: Together, these results indicate that HLE produces dose-related antinociception in several models of chemical and thermal pain, without tolerance induction, through mechanisms that involve an interaction with adenosinergic, muscarinic cholinergic, and opioid pathways. PMID:21966159

Woode, Eric; Abotsi, Wonder K. M.

2011-01-01

228

Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass (Lolium multiflorum Lam.).  

PubMed

Bacterial wilt caused by Xanthomonas translucens pv. graminis (Xtg) is a major disease of economically important forage crops such as ryegrasses and fescues. Targeted breeding based on seedling inoculation has resulted in cultivars with considerable levels of resistance. However, the mechanisms of inheritance of resistance are poorly understood and further breeding progress is difficult to obtain. This study aimed to assess the relevance of the seedling screening in the glasshouse for adult plant resistance in the field and to investigate genetic control of resistance to bacterial wilt in Italian ryegrass (Lolium multiflorum Lam.). A mapping population consisting of 306 F1 individuals was established and resistance to bacterial wilt was assessed in glasshouse and field experiments. Highly correlated data (r = 0.67-0.77, P < 0.01) between trial locations demonstrated the suitability of glasshouse screens for phenotypic selection. Analysis of quantitative trait loci (QTL) based on a high density genetic linkage map consisting of 368 amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers revealed a single major QTL on linkage group (LG) 4 explaining 67% of the total phenotypic variance (Vp). In addition, a minor QTL was observed on LG 5. Field experiments confirmed the major QTL on LG 4 to explain 43% (in 2004) to 84% (in 2005) of Vp and also revealed additional minor QTLs on LG 1, LG 4 and LG 6. The identified QTLs and the closely linked markers represent important targets for marker-assisted selection of Italian ryegrass. PMID:16799808

Studer, Bruno; Boller, Beat; Herrmann, Doris; Bauer, Eva; Posselt, Ulrich K; Widmer, Franco; Kölliker, Roland

2006-08-01

229

Bioactive endophytic fungi isolated from Caesalpinia echinata Lam. (Brazilwood) and identification of beauvericin as a trypanocidal metabolite from Fusarium sp.  

PubMed Central

Aiming to identify new sources of bioactive secondary metabolites, we isolated 82 endophytic fungi from stems and barks of the native Brazilian tree Caesalpinia echinata Lam. (Fabaceae). We tested their ethyl acetate extracts in several in vitro assays. The organic extracts from three isolates showed antibacterial activity against Staphylococcus aureus and Escherichia coli [minimal inhibitory concentration (MIC) 32-64 ?g/mL]. One isolate inhibited the growth of Salmonella typhimurium (MIC 64 ?g/mL) and two isolates inhibited the growth of Klebsiella oxytoca (MIC 64 ?g/mL), Candida albicans and Candida tropicalis (MIC 64-128 ?g/mL). Fourteen extracts at a concentration of 20 ?g/mL showed antitumour activities against human breast cancer and human renal cancer cells, while two isolates showed anti-tumour activities against human melanoma cancer cells. Six extracts were able to reduce the proliferation of human peripheral blood mononuclear cells, indicating some degree of selective toxicity. Four isolates were able to inhibit Leishmania (Leishmania) amazonensis and one isolate inhibited Trypanosoma cruzi by at least 40% at 20 ?g/mL. The trypanocidal extract obtained from Fusarium sp. [KF611679] culture was subjected to bioguided fractionation, which revealed beauvericin as the compound responsible for the observed toxicity of Fusarium sp. to T. cruzi. This depsipeptide showed a half maximal inhibitory concentration of 1.9 ?g/mL (2.43 ?M) in a T. cruzi cellular culture assay. PMID:25742265

Campos, Fernanda Fraga; Sales, Policarpo A; Romanha, Alvaro José; Araújo, Márcio SS; Siqueira, Ezequias P; Resende, Jarbas M; Alves, Tânia MA; Martins-Filho, Olindo A; dos Santos, Vera Lúcia; Rosa, Carlos A; Zani, Carlos L; Cota, Betania Barros

2015-01-01

230

An ethyl acetate fraction of Moringa oleifera Lam. Inhibits human macrophage cytokine production induced by cigarette smoke.  

PubMed

Moringa oleifera Lam. (MO) has been reported to harbor anti-oxidation and anti-inflammatory activity and useful in the treatment of inflammatory diseases. However, despite these findings there has been little work done on the effects of MO on immune cellular function. Since macrophages, TNF and related cytokines play an important pathophysiologic role in lung damage induced by cigarette smoke, we examined the effects of MO on cigarette smoke extract (CSE)-induced cytokine production by human macrophages. An ethyl acetate fraction of MO (MOEF) was prepared from fresh leaves extract of Moringa and shown to consist of high levels of phenolic and antioxidant activities. Human monocyte derived macrophages (MDM) pre-treated with varying concentrations of MOEF showed decreased production of TNF, IL-6 and IL-8 in response to both LPS and CSE. The decrease was evident at both cytokine protein and mRNA levels. Furthermore, the extract inhibited the expression of RelA, a gene implicated in the NF-?B p65 signaling in inflammation. The findings highlight the ability of MOEF to inhibit cytokines (IL-8) which promote the infiltration of neutrophils into the lungs and others (TNF, IL-6) which mediate tissue disease and damage. PMID:24553063

Kooltheat, Nateelak; Sranujit, Rungnapa Pankla; Chumark, Pilaipark; Potup, Pachuen; Laytragoon-Lewin, Nongnit; Usuwanthim, Kanchana

2014-01-01

231

Bioactive endophytic fungi isolated from Caesalpinia echinata Lam. (Brazilwood) and identification of beauvericin as a trypanocidal metabolite from Fusarium sp.  

PubMed

Aiming to identify new sources of bioactive secondary metabolites, we isolated 82 endophytic fungi from stems and barks of the native Brazilian tree Caesalpinia echinata Lam. (Fabaceae). We tested their ethyl acetate extracts in several in vitro assays. The organic extracts from three isolates showed antibacterial activity against Staphylococcus aureus and Escherichia coli [minimal inhibitory concentration (MIC) 32-64 ?g/mL]. One isolate inhibited the growth of Salmonella typhimurium (MIC 64 ?g/mL) and two isolates inhibited the growth of Klebsiella oxytoca (MIC 64 ?g/mL), Candida albicans and Candida tropicalis (MIC 64-128 ?g/mL). Fourteen extracts at a concentration of 20 ?g/mL showed antitumour activities against human breast cancer and human renal cancer cells, while two isolates showed anti-tumour activities against human melanoma cancer cells. Six extracts were able to reduce the proliferation of human peripheral blood mononuclear cells, indicating some degree of selective toxicity. Four isolates were able to inhibit Leishmania (Leishmania) amazonensis and one isolate inhibited Trypanosoma cruzi by at least 40% at 20 ?g/mL. The trypanocidal extract obtained from Fusarium sp. [KF611679] culture was subjected to bioguided fractionation, which revealed beauvericin as the compound responsible for the observed toxicity of Fusarium sp. to T. cruzi. This depsipeptide showed a half maximal inhibitory concentration of 1.9 ?g/mL (2.43 ?M) in a T. cruzi cellular culture assay. PMID:25742265

Campos, Fernanda Fraga; Sales Junior, Policarpo A; Romanha, Alvaro José; Araújo, Márcio Ss; Siqueira, Ezequias P; Resende, Jarbas M; Alves, Tânia Ma; Martins-Filho, Olindo A; Santos, Vera Lúcia Dos; Rosa, Carlos A; Zani, Carlos L; Cota, Betania Barros

2015-02-01

232

Effect of Butea monosperma Lam. leaves and bark extracts on blood glucose in streptozotocin-induced severely diabetic rats  

PubMed Central

Background: Diabetes mellitus is a chronic metabolic disorder that has significant impact on the health, quality of life and life expectancy, as well as on the health care system. Butea monosperma Lam. Kuntze (Fabaceae), commonly known as palash, is widely used in the treatment of various diseases and disorders including diabetes. Materials and Methods: The present study was planned to evaluate the antidiabetic effect of aqueous extracts of B. monosperma leaves and bark in streptozotocin-induced severely diabetic rats. The animals were divided into four groups, with each consisting of six rats, viz. control, diabetic control, leaf extract-treated and bark extract-treated groups. Treatment was continued for 6 weeks. The biochemical estimations included blood glucose and serum insulin levels. Histopathology of pancreas was also performed. Results: The results indicated that both leaf and bark extracts of B. monosperma produced insignificant antihyperglycemic activity. The leaf and bark extracts reduced blood glucose to an extent of 28% and 11%, respectively. It was also evidenced that both leaf and bark extracts did not increase insulin synthesis or secretion and did not improve pancreatic architecture as reflected by the histopathologic studies. Conclusions: The findings of the study emphasize that B. monosperma does not possess significant antidiabetic activity in severe experimental diabetes at the dosage tested. PMID:22224059

Ahmed, Faiyaz; Siddaraju, N. S.; Harish, M.; Urooj, Asna

2012-01-01

233

Overexpression of a LAM domain containing RNA-binding protein LARP1c induces precocious leaf senescence in Arabidopsis.  

PubMed

Leaf senescence is the final stage of leaf life history, and it can be regulated by multiple internal and external cues. La-related proteins (LARPs), which contain a well-conserved La motif (LAM) domain and normally a canonical RNA recognition motif (RRM) or noncanonical RRM-like motif, are widely present in eukaryotes. Six LARP genes (LARP1a-1c and LARP6a-6c) are present in Arabidopsis, but their biological functions have not been studied previously. In this study, we investigated the biological roles of LARP1c from the LARP1 family. Constitutive or inducible overexpression of LARP1c caused premature leaf senescence. Expression levels of several senescence-associated genes and defense-related genes were elevated upon overexpression of LARP1c. The LARP1c null mutant 1c-1 impaired ABA-, SA-, and MeJA-induced leaf senescence in detached leaves. Gene expression profiles of LARP1c showed age-dependent expression in rosette leaves. Taken together, our results suggest LARP1c is involved in regulation of leaf senescence. PMID:22965746

Zhang, Bangyue; Jia, Jianheng; Yang, Min; Yan, Chunxia; Han, Yuzhen

2012-10-01

234

Overexpression of a LAM Domain Containing RNA-Binding Protein LARP1c Induces Precocious Leaf Senescence in Arabidopsis  

PubMed Central

Leaf senescence is the final stage of leaf life history, and it can be regulated by multiple internal and external cues. La-related proteins (LARPs), which contain a well-conserved La motif (LAM) domain and normally a canonical RNA recognition motif (RRM) or noncanonical RRM-like motif, are widely present in eukaryotes. Six LARP genes (LARP1a-1c and LARP6a-6c) are present in Arabidopsis, but their biological functions have not been studied previously. In this study, we investigated the biological roles of LARP1c from the LARP1 family. Constitutive or inducible overexpression of LARP1c caused premature leaf senescence. Expression levels of several senescence-associated genes and defense-related genes were elevated upon overexpression of LARP1c. The LARP1c null mutant 1c-1 impaired ABA-, SA-, and MeJA-induced leaf senescence in detached leaves. Gene expression profiles of LARP1c showed age-dependent expression in rosette leaves. Taken together, our results suggest LARP1c is involved in regulation of leaf senescence. PMID:22965746

Zhang, Bangyue; Jia, Jianheng; Yang, Min; Yan, Chunxia; Han, Yuzhen

2012-01-01

235

Identification of a Proteinaceous Component in the Leaf of Moringa Oleifera lam. with Effects on High Serum Creatinine.  

PubMed

Moringa oleifera Lam. has been an important plant in the history of mankind, both for its nutritional and medicinal uses. Apart from bactericidal effects, the parts of this plant have been effectively used in the treatment of circulatory, respiratory, endocrine, digestive as well as neural disorders. Till date, though, there has been no reported activity of the involvement of any proteinaceous extract from M. oleifera on high levels of serum creatinine. To address this issue, blood samples with high levels of serum creatinine (2 mg/dl and above) were treated with leaf extract from M. oleifera. The crude extract was partially purified initially and eventually purified to completion as well. All these proteinaceous fractions were used to treat samples with high levels of serum creatinine as mentioned above. While the treatment of serum sample having high creatinine with crude extract and partially purified protein fractions showed a decrease of approximately 20% in the levels of serum creatinine over a period of 24 h, the samples treated with purified protein fraction reduced the serum creatinine level by 50%. In light of the fact that increased level of serum creatinine levels have adverse downstream effects on the heart, lungs and other organs, this communication assumes significance because it suggests a way of reducing the level of serum creatinine as an emergency measure. Further, the identification and characterisation of this proteinaceous component and possible in vivo experiments would provide a major tool for the treatment of downstream complications associated with increased serum creatinine via a new sources, albeit a natural one. PMID:24799742

Sahoo, S; Raghavendra, K M; Biswas, S

2014-01-01

236

Improvement of the coagulation/flocculation process using a combination of Moringa oleifera lam with anionic polymer in water treatment.  

PubMed

The objective of this study is to investigate the impacts of anionic polymer as a flocculant aid on the coagulation/flocculation performance with a saline solution of Moringa oleifera as a coagulant to provide larger flocs and decrease the time sedimentation. For the tests, raw water was used from Pirapó River Basin (Maringá, Paraná, Brazil). Optimization of coagulation/flocculation tests was initially performed in a jar-test with a dosage of M. oleifera Lam (crude extract--MO, oil-extracted with ethanol--MO (et) and hexane--MO (hex) 1% m/v) as the coagulant that ranged from 10 to 60 mg L(-1) and of the anionic polymer 0.1% as a flocculant aid with a dosage that ranged from 0 to 0.4 mg L(-1). The parameters analysed were colour, turbidity and compounds with absorption in UV254nm. In view of the statistical analysis results, MO (hex) with a dosage of 30 mg L(-1) was chosen as a coagulant for the next tests of coagulation/flocculation. When anionic polymer was used alone (0.0 mg L(-1) of MO (hex)), parameters were not removed and there was no generation of heavy flocs as compared with the combination of MO (hex) with the anionic polymer. Statistical analysis showed that MO (hex) obtained the highest removals of the parameters analysed in lower dosages and no significant increase in parameters removal was observed when the polymer dosage was increased. The efficacy of the coagulant +/- anionic polymer was optimal when 30mg L(-1) of MO (hex) was used as a coagulant and 0.1 mg L(-1) of the anionic polymer was used as a flocculant aid, decreasing the time sedimentation from 1 h to 15 min. PMID:25145175

Bongiovani, Milene Carvalho; Camacho, Franciele Pereira; Nishi, Letícia; Coldebella, Priscila Ferri; Valverde, Karina Cardoso; Vieira, Angélica Marquetotti Salcedo; Bergamasco, Rosângela

2014-01-01

237

A comparative study of proteomic differences between pencil and storage roots of sweetpotato (Ipomoea batatas (L.) Lam.).  

PubMed

Fibrous roots of sweetpotato (Ipomoea batatas (L.) Lam.) usually develop into both pencil and storage roots. To understand protein function in root development, a proteomic analysis was conducted on the pencil and storage roots of the light orange-fleshed sweetpotato cultivar, Yulmi. Two-dimensional gel electrophoresis showed that expression of 30 protein spots differed between pencil and storage roots: 15 proteins were up-regulated or expressed in pencil roots and 15 in storage roots. Differentially expressed proteins spots were investigated using matrix-assisted laser desorption/ionization time of flight mass spectrometry, and 10 proteins from pencil roots were identified as binding protein isoform A, catechol oxidase, peroxidases, ascorbate peroxidase, endochitinase, flavanone 3-hydroxylase and unknown proteins. Of the proteins up-regulated in, or restricted to, storage roots, 13 proteins were identified as protein disulfide isomerase, anionic peroxidase, putative ripening protein, sporamin B, sporamin A and sporamin A precursor. An analysis of enzyme activity revealed that catechol oxidase and peroxidase as the first and last enzymes of the lignin biosynthesis pathway, and ascorbate peroxidase had higher activities in pencil than in storage roots. The total concentration of phenolic compounds was also far higher in pencil than in storage roots, and lignin accumulated only in pencil roots. These results provide important insight into sweetpotato proteomics, and imply that lignin biosynthesis and stress-related proteins are up-regulated or uniquely expressed in pencil roots. The results indicate that the reduction of carbon flow toward phenylpropanoid biosynthesis and its delivery to carbohydrate metabolism is a major event in storage root formation. PMID:25562766

Lee, Jeung Joo; Kim, Yun-Hee; Kwak, Youn-Sig; An, Jae Young; Kim, Pil Joo; Lee, Byung Hyun; Kumar, Vikranth; Park, Kee Woong; Chang, Eun Sil; Jeong, Jae Cheol; Lee, Haeng-Soon; Kwak, Sang-Soo

2015-02-01

238

Effect of Moringa oleifera Lam. seed extract on toluene diisocyanate-induced immune-mediated inflammatory responses in rats.  

PubMed

Moringa oleifera Lam. is a small tree cultivated throughout India. We have investigated the effect of ethanolic extract of seeds of Moringa oleifera (MOEE, an herbal remedy) on the potential prevention of immune-mediated inflammatory responses in toluene diisocyanate (TDI as antigen)-induced asthma in Wistar rats. Rats were divided into five different groups (n = 8/group): Group-I = unsensitized control; Group-II = TDI control/vehicle; Group-III = dexamethasone (DXM) 2.5 mg/kg; and, Groups IV and V = 100 mg/kg and 200 mg/kg body weight [BW] of MOEE, respectively. All rats (except unsensitized controls) were sensitized by intranasal application of 10% TDI to induce airway hypersensitivity. Animals in Groups II-V were given their respective drug treatment per os from 1 wk prior to initiation of sensitization until the day of final provocation with 5% TDI. After this last challenge, all rats were examined for hyperreactivity symptoms and then sacrificed to determine their total and differential leucocytes in blood and bronchoalveolar (BAL) fluid and levels of TNF proportional, variant, IL-4, and IL-6 in their BAL and serum. Homogenates of one lung lobe from each animal were utilized to assess oxidative stress; a separate lobe underwent histologic examination to assess airway inflammatory status. The results suggest that asthmatic symptoms were found in TDI control rats only, while both MOEE- and DXM-treated rats did not manifest any airway abnormality. In MOEE- and DXM-treated rats, neutrophil and eosinophil levels in the blood were decreased significantly; levels of total cells and each different cell in their BAL fluid were markedly decreased as compared to those in TDI controls. TNF alpha, IL-4, and IL-6 were predominant in serum as well as in BAL fluids in TDI controls, but these levels were reduced significantly by MOEE treatment. The antioxidant activity in relation to antiinflammatory activity of the extract and histopathological observations also reflected a protective effect. Based on the above findings and observations, it can be concluded that Moringa oleifera may possess some beneficial properties that act against chemically stimulated immune-mediated inflammatory responses that are characteristic of asthma in the rat. PMID:18958717

Mahajan, Shailaja G; Mali, Ravindra G; Mehta, Anita A

2007-04-01

239

Suppression of seed bruchid ( Callosobruchus maculatus F.) development and damage on cowpea ( Vigna unguiculata (L.) Walp.) with Zanthoxylum zanthoxyloides (Lam.) Waterm. (Rutaceae) root bark powder when compared to neem seed powder and pirimiphos-methyl  

Microsoft Academic Search

Reproduction suppression properties in Zanthoxylum zanthoxyloides (Lam.) Waterm. (Rutaceae) root bark powder (ZRBP) against the cowpea seed bruchid (CSB), Callosobruchus maculatus F., were evaluated against neem (Azadirachta indica A. Juss.) seed powder (NSP) and pirimiphos-methyl (PM) (Actellic 2% Dust, ICI). At application rates ranging from 0.125 to 3 g per 20 g seed, ZRBP was as effective an oviposition suppressant

E. O. Ogunwolu; A. T. Odunlami

1996-01-01

240

Symmetry-breaking 60°-spin order in the A-site-ordered perovskite LaM n3V4O12  

NASA Astrophysics Data System (ADS)

The magnetism of the A-site-ordered perovskite LaM n3V4O12 is studied comprehensively by means of neutron powder diffraction experiments and theoretical calculations. Magnetic neutron diffraction results show that a rhombohedral 60° spin structure emerges on the cubic lattice below a 44-K Néel transition. Ab initio electronic structure calculations confirm that high-spin Mn2 + moments are localized while V 3 d -band states are itinerant, and that the noncollinear 60° spin structure is more stable than collinear ferromagnetic or G-type antiferromagnetic alternatives. Effective Heisenberg model calculations reveal that the appearance of such a nontrivial spin structure can be attributed to significant next-nearest-neighbor and third-nearest-neighbor magnetic interactions.

Saito, Takashi; Toyoda, Masayuki; Ritter, Clemens; Zhang, Shoubao; Oguchi, Tamio; Attfield, J. Paul; Shimakawa, Yuichi

2014-12-01

241

Determine TB-LAM lateral flow urine antigen assay for HIV-associated tuberculosis: recommendations on the design and reporting of clinical studies  

PubMed Central

Detection of the Mycobacterium tuberculosis cell wall antigen lipoarabinomannan (LAM) in urine permits diagnoses of tuberculosis (TB) to be made in HIV-infected patients with advanced immunodeficiency. This can be achieved at the point-of-care within just 30 minutes using the Determine TB-LAM, which is a commercially available, lateral-flow urine ‘strip test’ assay. The assay has been shown to have useful diagnostic accuracy in patients enrolling in antiretroviral treatment services or in HIV-infected patients requiring admission to hospital medical wards in sub-Saharan Africa. Such patients have high mortality risk and have most to gain from rapid diagnosis of TB and immediate initiation of treatment. However, few studies using this assay have yet been reported and many questions remain concerning the correct use of the assay, interpretation of results, the role of the assay as an add-on test within existing diagnostic algorithms and the types of further studies needed. In this paper we address a series of questions with the aim of informing the design, conduct and interpretation of future studies. Specifically, we clarify which clinical populations are most likely to derive benefit from use of this assay and how patients enrolled in such studies might best be characterised. We describe the importance of employing a rigorous microbiological diagnostic reference standard in studies of diagnostic accuracy and discuss issues surrounding the specificity of the assay in different geographical areas and potential cross-reactivity with non-tuberculous mycobacteria and other organisms. We highlight the importance of careful procedures for urine collection and storage and the critical issue of how to read and interpret the test strips. Finally, we consider how the assay could be used in combination with other assays and outline the types of studies that are required to build the evidence base concerning its use. PMID:24004840

2013-01-01

242

Spectres infrarouge et Raman de l'acide stéarique et d'une série d'acides gras forme C: modes de squelette, couplage des modes longitudinaux acoustiques (LAM1, LAM3) avec les modes dans le plan de la liaison hydrogène du dimère en dessous de 700 cm -1  

NASA Astrophysics Data System (ADS)

A vibrational study of the skeletal modes of stearic acid ( n = 18) in the C form is interpreted on the basis of the calculated dispersion curves for the aliphatic chain C n H n + 2 . The Raman spectra below 200 cm -1, obtained at 90 K, display one very intense band at 70 cm -1 that is assigned to the longitudinal acoustic mode LAM1. The observed frequencies, together with those of LAM3, follow accurately the selection rules of those expected for a C 36 alkane chain of structure C2h. This led us to consider the overall dimer as a molecular entity located on a Ci, site of the crystal. The result, in agreement with the calculations of Zerbi, can be extended to the entire set of acids studied ( n = 26, 23, 16, 14, 12). The observed coupling between the vibrational skeletal modes of the two chains and the in-plane modes of the hydrogen bonded dimer ?(OH⋯O) and ?(O⋯O) is responsible for the behaviour of the C form. Finally, we present the description of the vibrational modes and their classification in the different symmetry classes for polymethylene and paraffins ( neven) accounting for the conventions used in this work and in the literature.

Vogel-Weill, C.; Corset, J.

1995-11-01

243

Determination of partition coefficients for trace elements in high pressure-temperature experimental run products by laser ablation microprobe-inductively coupled plasma-mass spectrometry (LAM-ICP-MS)  

Microsoft Academic Search

This paper reports the first trace element partition coefficients measured on experimentally produced products (clinopyroxene, garnet, rutile, and glass) by laser ablation microprobe-inductively coupled plasma-mass spectrometry (LAM-ICP-MS). A 266 nm (UV) laser microprobe was used to improve ablation characteristics and to achieve a fourfold reduction in ablation pit diameter compared to the previously used 1064 nm beam. Results are compared

G. A. Jenner; S. E. Jackson; B. J. Fryer; H. P. Longerich; S. F. Foley; T. H. Green

1993-01-01

244

Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds: A Potential Tool to Access the Mobilization of Seed Storage Proteins  

PubMed Central

Several proteins have been isolated from seeds of leguminous, but this is the first report that a protease was obtained from seeds of Caesalpinia echinata Lam., a tree belonging to the Fabaceae family. This enzyme was purified to homogeneity by hydrophobic interaction and anion exchange chromatographies and gel filtration. This 61-kDa serine protease (CeSP) hydrolyses H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide (Km 55.7??M) in an optimum pH of 7.1, and this activity is effectively retained until 50°C. CeSP remained stable in the presence of kosmotropic anions (PO4?3?, SO4?2?, and CH3COO?) or chaotropic cations (K+ and Na+). It is strongly inhibited by TLCK, a serine protease inhibitor, but not by E-64, EDTA or pepstatin A. The characteristics of the purified enzyme allowed us to classify it as a serine protease. The role of CeSP in the seeds cannot be assigned yet but is possible to infer that it is involved in the mobilization of seed storage proteins. PMID:22629147

Praxedes-Garcia, Priscila; Cruz-Silva, Ilana; Gozzo, Andrezza Justino; Abreu Nunes, Viviane; Torquato, Ricardo José; Tanaka, Aparecida Sadae; Figueiredo-Ribeiro, Rita de Cássia; Gonzalez, Yamile Gonzalez; Araújo, Mariana da Silva

2012-01-01

245

Evaluation of in vitro antimalarial activity of different extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and fractions of the most potent extracts.  

PubMed

Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro?? ? -hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36±0.01 and 1.83±0.03?mg/mL and IC90 values of 2.12±0.04 and 2.62±0.09?mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47±0.006 and 0.71±0.006?mg/mL, respectively. PMID:24558335

Mojarrab, Mahdi; Shiravand, Ali; Delazar, Abbas; Heshmati Afshar, Fariba

2014-01-01

246

Stress responses of duckweed (Lemna minor L.) and water velvet (Azolla filiculoides Lam.) to anionic surfactant sodium-dodecyl-sulphate (SDS).  

PubMed

Surfactants are used for several purposes and recently they have attracted the attention for their ability to modify the behavior of other preexistent or co-disposed contaminants, although their use or discharge in wastewaters can represent a real or potential risk for the environment. Lemna minor L. and Azolla filiculoides Lam. are floating aquatic macrophytes, very effective in accumulating several pollutants including sodium dodecyl sulphate (SDS). In this work we evaluated the effects of SDS on these species by determining the stress ethylene production via laser-based trace gas detection, and the activities of enzymes involved in stress response, such as guaiacol peroxidase (G-POD), phenylalanine ammonia-lyase (PAL) and polyphenol-oxidase (PPO). Phenolics content was also determined. The macrophytes were treated with different concentrations of SDS for one week. SDS affected duckweed enzymatic activities and phenol content. While in the fern phenolics amount, PAL, G-POD and PPO activities were not affected by SDS except for 100 ppm SDS, the only concentration that was taken up and not completely degraded. Stress ethylene production was induced only in the fern treated with 50 and 100 ppm SDS. PMID:22277247

Forni, C; Braglia, R; Harren, F J M; Cristescu, S M

2012-04-01

247

Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam.  

PubMed

Medicinal plants have been shown to have both chemopreventive and/or therapeutic effects on cancer and other diseases related to oxidative damage. Moringa oleifera Lam., known in the Hausa and Igala languages of Nigeria as "Zogale" and "Gergedi," respectively, and drumstick in English, is a plant that is used both as food and in folkloric medicine in Nigeria and elsewhere. Different parts of the plant were analyzed for polyphenol content as well as in vitro antioxidant potential. The methanol extract of the leaves of M. oleifera contained chlorogenic acid, rutin, quercetin glucoside, and kaempferol rhamnoglucoside, whereas in the root and stem barks, several procyanidin peaks were detected. With the xanthine oxidase model system, all the extracts exhibited strong in vitro antioxidant activity, with 50% inhibitory concentration (IC(50)) values of 16, 30, and 38 microL for the roots, leaves, and stem bark, respectively. Similarly, potent radical scavenging capacity was observed when extracts were evaluated with the 2-deoxyguanosine assay model system, with IC(50) values of 40, 58, and 72 microL for methanol extracts of the leaves, stem, and root barks, respectively. The high antioxidant/radical scavenging effects observed for different parts of M. oleifera appear to provide justification for their widespread therapeutic use in traditional medicine in different continents. The possibility that this high antioxidant/radical scavenging capacity may impact on the cancer chemopreventive potential of the plant must be considered. PMID:20521992

Atawodi, Sunday E; Atawodi, Joy C; Idakwo, Gabriel A; Pfundstein, Beate; Haubner, Roswitha; Wurtele, Gerd; Bartsch, Helmut; Owen, Robert W

2010-06-01

248

Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves.  

PubMed

Water, aqueous methanol, and aqueous ethanol extracts of freeze-dried leaves of Moringa oleifera Lam. from different agroclimatic regions were examined for radical scavenging capacities and antioxidant activities. All leaf extracts were capable of scavenging peroxyl and superoxyl radicals. Similar scavenging activities for different solvent extracts of each collection were found for the stable 1,1-diphenyl 2-picrylhydrazyl (DPPH(*)) radical. Among the three different moringa samples, both methanol and ethanol extracts of Indian origins showed the highest antioxidant activities, 65.1 and 66.8%, respectively, in the beta-carotene-linoleic acid system. Nonetheless, increasing concentration of all the extracts had significantly (P < 0.05) increased reducing power, which may in part be responsible for their antioxidant activity. The major bioactive compounds of phenolics were found to be flavonoid groups such as quercetin and kaempferol. On the basis of the results obtained, moringa leaves are found to be a potential source of natural antioxidants due to their marked antioxidant activity. This is the first report on the antioxidant properties of the extracts from freeze-dried moringa leaves. Overall, both methanol (80%) and ethanol (70%) were found to be the best solvents for the extraction of antioxidant compounds from moringa leaves. PMID:12670148

Siddhuraju, Perumal; Becker, Klaus

2003-04-01

249

Antioxidant activities of trypsin inhibitor, a 33 KDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57).  

PubMed

Trypsin inhibitors (TIs), root storage proteins, were purified from sweet potato (Ipomoea batatas[L.] Lam cv. Tainong 57) roots by trypsin affinity column according to the methods of Hou and Lin (Plant Sci. 1997, 126, 11-19 and Plant Sci. 1997, 128, 151-158). A single band of 33 kDa TI was obtained by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. This purified 33 kDa TI had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. There was positive correlation between scavenging effects against DPPH (2 to 22%) and amounts of 33 kDa TI (1.92 to 46 pmol). The scavenging activities of 33 kDa TI against DPPH were calculated from linear regression to be about one-third of those of glutathione between 5 and 80 pmol. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detection, it was found that 33 kDa TI could capture hydroxyl radical, and the intensities of EPR signal were significantly decreased from 1.5 to 6 pmol of 33 kDa TI compared to those of the controls. It is suggested that 33 kDa TI, one of the sweet potato root storage proteins, may play a role as an antioxidant in roots and may be beneficial to health when it is consumed. PMID:11409996

Hou, W C; Chen, Y C; Chen, H J; Lin, Y H; Yang, L L; Lee, M H

2001-06-01

250

Effect of charged residue substitutions on the thermodynamics of signal peptide-lipid interactions for the Escherichia coli LamB signal sequence.  

PubMed Central

We have used tryptophan fluorescence spectroscopy to characterize the binding affinities of an Escherichia coli LamB signal peptide family for lipid vesicles. These peptides harbor charged residue substitutions in the hydrophobic core region. Titrations of peptides with vesicles composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine and 1-palmitoyl-2-oleoyl-sn-3-phosphoglycerol (65:35 mol%), in conjunction with evaluation of peptide dissociation rates from these vesicles, were used to determine binding parameters quantitatively. We find that under low ionic strength conditions, point mutations introducing negatively charged aspartate residues substantially reduce peptide affinity relative to the wild-type peptide. However, the difference between wild-type and mutant peptide affinities was much lower under approximately physiological ionic strength. In addition, the lipid affinities of model surface-binding and transmembrane peptides were determined. These comparative studies with signal and model peptides permitted semi-quantitative deconvolution of signal peptide binding into electrostatic and hydrophobic components. We find that both interactions contribute significantly to binding, although the theoretically available hydrophobic free energy is largely offset by unfavorable polar-group effects. The implications of these results for understanding the potential roles of the signal sequence in protein translocation are discussed. PMID:7819487

Jones, J D; Gierasch, L M

1994-01-01

251

Inhibitory effect of n-butanol fraction of Moringa oleifera Lam. seeds on ovalbumin-induced airway inflammation in a guinea pig model of asthma.  

PubMed

Moringaceae, which belongs to the Moringa oleifera Lam. family, is a well-known herb used in Asian medicine as an antiallergic drug. In the present study, the efficacy of the n-butanol extract of the seeds of the plant (MONB) is examined against ovalbumin-induced airway inflammation in guinea pigs. The test drugs (MONB or dexamethasone) are administered orally prior to challenge with aerosolized 0.5% ovalbumin. During the experimental period, bronchoconstriction tests are performed, and lung function parameters are measured. The blood and bronchoalveolar lavage fluid are collected to assess cellular content, and serum is used for cytokine (tumor necrosis factor-alpha, interleukin-4, and interleukin-6) assays. Histamine assays of lung tissue are performed using lung tissue homogenate. The results suggest that in ovalbumin-sensitized model control animals, tidal volume is decreased, respiration rate is increased, and both the total and differential cell counts in blood and bronchoalveolar lavage fluid are increased significantly compared with nonsensitized controls. MONB treatment shows improvement in all parameters except bronchoalveolar lavage tumor necrosis factor-alpha and interleukin-4. Moreover, MONB treatment demonstrates protection against acetylcholine-induced bronchoconstriction and airway inflammation. These results indicate that MONB has an inhibitory effect on airway inflammation. Thus, MONB possesses an antiasthmatic property through modulation of the relationship between Th1/Th2 cytokine imbalances. PMID:19966143

Mahajan, Shailaja G; Banerjee, Aryamitra; Chauhan, Bhupendrasinh F; Padh, Harish; Nivsarkar, Manish; Mehta, Anita A

2009-01-01

252

Do Ecological Niche Model Predictions Reflect the Adaptive Landscape of Species?: A Test Using Myristica malabarica Lam., an Endemic Tree in the Western Ghats, India  

PubMed Central

Ecological niche models (ENM) have become a popular tool to define and predict the “ecological niche” of a species. An implicit assumption of the ENMs is that the predicted ecological niche of a species actually reflects the adaptive landscape of the species. Thus in sites predicted to be highly suitable, species would have maximum fitness compared to in sites predicted to be poorly suitable. As yet there are very few attempts to address this assumption. Here we evaluate this assumption. We used Bioclim (DIVA GIS version 7.3) and Maxent (version 3.3.2) to predict the habitat suitability of Myristica malabarica Lam., an economically important tree occurring in the Western Ghats, India. We located populations of the trees naturally occurring in different habitat suitability regimes (from highly suitable to poorly suitable) and evaluated them for their regeneration ability and genetic diversity. We also evaluated them for two plant functional traits, fluctuating asymmetry – an index of genetic homeostasis, and specific leaf weight – an index of primary productivity, often assumed to be good surrogates of fitness. We show a significant positive correlation between the predicted habitat quality and plant functional traits, regeneration index and genetic diversity of populations. Populations at sites predicted to be highly suitable had a higher regeneration and gene diversity compared to populations in sites predicted to be poor or unsuitable. Further, individuals in the highly suitable sites exhibited significantly less fluctuating asymmetry and significantly higher specific leaf weight compared to individuals in the poorly suitable habitats. These results for the first time provide an explicit test of the ENM with respect to the plant functional traits, regeneration ability and genetic diversity of populations along a habitat suitability gradient. We discuss the implication of these resultsfor designing viable species conservation and restoration programs. PMID:24312402

Nagaraju, Shivaprakash K.; Gudasalamani, Ravikanth; Barve, Narayani; Ghazoul, Jaboury; Narayanagowda, Ganeshaiah Kotiganahalli; Ramanan, Uma Shaanker

2013-01-01

253

In vivo and in vitro immunomodulatory potential of swertiamarin isolated from Enicostema axillare (Lam.) A. Raynal that acts as an anti-inflammatory agent.  

PubMed

Swertiamarin is a secoiridoid glycoside found in Enicostema axillare (Lam) A. Raynal, a medicinal plant used as a depurative in the Indian system of traditional medicine. The present study evaluated the immunomodulatory activity of isolated swertiamarin. In vivo immunomodulatory activity of swertiamarin (2, 5, and 10 mg/kg b.w.) was evaluated in a model of sheep red blood cells (SRBC) by assessing its effect on organ weight, hemagglutinating antibody titer (HA), plaque-forming cells (PFC), quantitative hemolysis of SRBC, and delayed type hypersensitivity (DTH). In vitro immunomodulatory potential was studied on isolated splenocytes, neutrophils, and peritoneal macrophages. In silico immunomodulatory effects were evaluated by docking of swertiamarin on proinflammatory cytokines to confirm its potential. In in vivo studies, the animals treated with swertiamarin showed a significant (P ? 0.05) increase in antibody titer, plaque-forming cells, and also in weight of the thymus and spleen. A decreased response to DTH reaction was recorded with the treatment of swertiamarin. In in vitro studies, treatment with swertiamarin modulated the messenger RNA (mRNA) and protein expression of IFN-?, IL-10, and IL-4 significantly (P ? 0.05) and also favored Th2-mediated response on concanavalin A (Con A)-induced splenocytes. The compound inhibited the release of free radicals significantly (P ? 0.05) in phytohemagglutinin (PHA)-induced neutrophils and also ameliorated the mRNA and protein expression of proinflammatory cytokines (TNF-?, IL-1?, and IL-6) in lipopolysaccharide (LPS)-induced macrophages. In in silico, the best docked pose of swertiamarin with the target proteins (TNF-?, IL-1?, and IL-6) was confirmed that swertiamarin acted as an anti-inflammatory mediator. PMID:24736879

Saravanan, S; Pandikumar, P; Prakash Babu, N; Hairul Islam, V I; Thirugnanasambantham, K; Gabriel Paulraj, M; Balakrishna, K; Ignacimuthu, S

2014-10-01

254

Effect of charged residue substitutions on the membrane-interactive properties of signal sequences of the Escherichia coli LamB protein.  

PubMed Central

Although the central role of the signal sequence in protein export is well established, the molecular details underlying signal sequence in vivo function remain unclear. As part of our continuing effort to relate signal sequence phenotypes to specific biophysical properties, we have carried out an extensive characterization of the secondary structure and lipid interactions for a family of peptides corresponding to the wild-type E. coli LamB signal sequence, and mutants that harbor charged residue point mutations in the hydrophobic core region. We used membrane-resident fluorescence quenching according to the parallax method to determine the relative depth of insertion of tryptophan-labeled analogs of these peptides into the acyl chain region of bilayer vesicles composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol. Also, restriction of acyl chain motion upon peptide binding was evaluated using steady-state fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene. Each of these peptides showed evidence of insertion into the acyl chain region, although most likely not in a transmembrane orientation. The mutant peptides were shown to have a reduced insertion potential relative to the wild-type peptide. Furthermore, tryptophan spectral properties indicated that insertion of the wild-type and mutant peptides enhances bilayer hydration. This effect was particularly pronounced with peptides harboring negatively charged aspartate point substitutions. The results are discussed in relation to the potential roles of signal sequences in mediating protein translocation. PMID:7819486

Jones, J D; Gierasch, L M

1994-01-01

255

Sveconorwegian crustal underplating in southwestern Fennoscandia: LAM-ICPMS U Pb and Lu Hf isotope evidence from granites and gneisses in Telemark, southern Norway  

NASA Astrophysics Data System (ADS)

Laser ablation ICPMS U-Pb and Lu-Hf isotope data on granitic-granodioritic gneisses of the Precambrian Vråvatn complex in central Telemark, southern Norway, indicate that the magmatic protoliths crystallized at 1201 ± 9 Ma to 1219 ± 8 Ma, from magmas with juvenile or near-juvenile Hf isotopic composition ( 176Hf/ 177Hf = 0.2823 ± 11, epsilon-Hf > + 6). These data provide supporting evidence for the depleted mantle Hf-isotope evolution curve in a time period where juvenile igneous rocks are scarce on a global scale. They also identify a hitherto unknown event of mafic underplating in the region, and provide new and important limits on the crustal evolution of the SW part of the Fennoscandian Shield. This juvenile geochemical component in the deep crust may have contributed to the 1.0-0.92 Ga anorogenic magmatism in the region, which includes both A-type granite and a large anorthosite-mangerite-charnockite-granite intrusive complex. The gneisses of the Vråvatn complex were intruded by a granitic pluton with mafic enclaves and hybrid facies (the Vrådal granite) in that period. LAM-ICPMS U-Pb data from zircons from granitic and hybrid facies of the pluton indicates an intrusive age of 966 ± 4 Ma, and give a hint of ca. 1.46 Ga inheritance. The initial Hf isotopic composition of this granite ( 176Hf/ 177Hf = 0.28219 ± 13, epsilon-Hf = - 5 to + 6) overlaps with mixtures of pre-1.7 Ga crustal rocks and juvenile Sveconorwegian crust, lithospheric mantle and/or global depleted mantle. Contributions from ca. 1.2 Ga crustal underplate must be considered when modelling the petrogenesis of late Sveconorwegian anorogenic magmatism in the region.

Andersen, T.; Griffin, W. L.; Sylvester, A. G.

2007-02-01

256

In vitro analysis on bactericidal screening and antioxidant potentiality of leaf and root extracts of Thottea siliquosa (Lam.) Ding Hou. An ethnobotanical plant  

PubMed Central

Objective Natural products of plant origin are potential source of novel antimicrobial and antioxidative agents. Thottea siliquosa (Lam.) Ding Hou. (T. siliquosa). A medicinal herb used by local tribals for treating various ailments. The present study aims at the phytochemical screening, GC-MS analysis, in vitro antibacterial activity and antioxidant potentiality of root and leaf extracts of T. siliquosa. Methods Hot continuous Soxhlet extraction, GC-MS analysis, antibacterial analysis by disc diffusion, microdilution assay and antioxidant potentialities by hydroxyl radical and nitric oxide radical scavenging. The data was statistically analyzed. Results Phytochemical screening of the ethyl acetate and methanolic extract of leaf and root revealed the presence of phenols, alkaloids, tannins and saponin. The extract revealed a pool of phytochemicals by comparison with authentic standards from spectral library. Both the extracts has shown their broad spectrum of inhibition against the selected bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia compared with standard antibiotic drug streptomycin. The extracts showed antioxidant activity by scavenging of free radicals such as hydroxyl and nitric oxide. The IC50 values of the ethyl acetate extracts leaf and root and standard in this assay were 167.5±0.67, 99.4±1.2, 192±2.5 µg/mL respectively. Similarly those methanolic extracts of leaf and root were 269.5±0.89 and 289.1±2.66 µg/mL respectively. Similarly, ethyl acetate and methanolic extracts also caused a moderate dose-dependent inhibition of nitric oxide with an IC50 range 65.5±1.55 to 148 ±3.09 µg/mL. The inhibitory activities were found to be dose dependent. Conclusion The present study provides evidence that ethyl acetate and methanol extract of leaf and root of T. siliquosa are potential source of natural antioxidants and bactericidal nature. It is essential that research should continue to isolate and purify the bio active components of this natural plant and use in drug discovery and development.

Nusaiba, Saiba Abdul Wahab; Murugan, Kumaraswamy

2013-01-01

257

Please cite this article in press as: Bradley, C.R., et al., Conservation genetics of Ireland's sole population of the River water crowfoot (Ranunculus fluitans Lam.). Aquat. Bot. (2013), http://dx.doi.org/10.1016/j.aquabot.2013.01.011  

E-print Network

Please cite this article in press as: Bradley, C.R., et al., Conservation genetics of Ireland.elsevier.com/locate/aquabot Conservation genetics of Ireland's sole population of the River water crowfoot (Ranunculus fluitans Lam's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, United Kingdom a r t i c l e i n f o

Provan, Jim

258

Ethnobotanical studies on Berberis aristata DC. root extracts  

Microsoft Academic Search

-3 mg\\/ml and for Aspergillus species, it was 3 ? 10 -3 mg\\/ml. All three extracts also had antifungal activity against the fungal species tested, except Candida krusei. The extracts of B. aristata also demonstrated anti-inflammatory, analgesic, and antipyretic activities. Chemical analysis revealed the presence of alkaloids, amino acids, tannins, terpenes, resins, phenols and reducing sugars as major compounds. FTIR-spectral

M. Shahid; T. Rahim; A. Shahzad; A. Latif; T. Fatma; M. Rashid; Adil Raza; S. Mustafa

2009-01-01

259

Bromelain treatment of human T cells removes CD44, CD45RA, E2/MIC2, CD6, CD7, CD8, and Leu 8/LAM1 surface molecules and markedly enhances CD2-mediated T cell activation.  

PubMed

Treatment of T cells with the cysteine protease bromelain has been widely used to enhance the binding of human T cells to human E (autologous E rosettes) and has been shown to remove surface T cell CD44 molecules. Ligand binding to CD44 has been shown to markedly augment T cell activation. To study the activation potential of bromelain-treated CD44 T cells, we have compared the proliferation of sham- and bromelain-treated normal human PBMC to mitogenic CD2 mAb. We found that bromelain not only removed T cell CD44, but also removed the CD45RA isoform of CD45 as well as E2/MIC2, CD6, CD7, CD8, and Leu 8/LAM1 molecules. T cell proliferation in response to CD2 mAb was increased 325% in bromelain-treated PBMC compared to sham-treated PBMC (p < 0.005). Reciprocal treatment experiments using purified T cells and monocytes demonstrated that the enhancement of T cell CD2 activation by bromelain occurred only when T cells were treated with bromelain and was accompanied by increased adhesion of T cells to monocytes. These data demonstrate that expression of portions of the extracellular domains of the CD44, CD45RA, E2/MIC2, CD6, CD7, CD8, and Leu 8/LAM1 surface molecules are not required for CD2 activation of human T cells. Rather, the removal of these surface molecules by bromelain is associated with enhanced T cell-monocyte aggregation and enhanced CD2-mediated T cell activation. Taken together with data that CD44, E2/MIC2, CD6, and CD7 mAb inhibit CD2/lymphocyte function-associated Ag-3-mediated cellular interactions and also augment CD2-mediated triggering of T cells, these data suggest that members of the bromelain-sensitive group of surface molecules may comprise a set of CD2-associated adhesion ligands that acts in concert to modulate human T cell activation. PMID:1281188

Hale, L P; Haynes, B F

1992-12-15

260

Wilbur A. Lam Title: Assistant Professor  

E-print Network

MEMs/microfabrication/microfluidics Hematology (platelet and leukocyte biology, hemostasis/thrombosis) Sickle cell disease Endothelial cell, is dedicated to applying and developing micro/nanotechnologies to study, diagnose, and treat blood disorders, "Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells," Nature

Garmestani, Hamid

261

Reasoning About Set Comprehensions Edmund S. L. Lam1  

E-print Network

University sllam@qatar.cmu.edu, iliano@cmu.edu Abstract Set comprehension is a mathematical notation-667-1-100, Effective Programming for Large Distributed Ensembles, from the Qatar National Research Fund (a member of the Qatar Foundation). The statements made herein are solely the responsibility of the authors. 1 #12;In

Cervesato, Iliano

262

M. Benosman LAM, Universite de Reims Champagne-Ardenne,  

E-print Network

-fixed time for planar flexible manipulators. We introduce a simple idea permitting the minimization of end-effector residual vibration when reaching a desired angular equilibrium position, in a pre-fixed desired travelling time. The results hold without considering internal elastic damping effect, using a classical

De Luca, Alessandro

263

Phytotoxic compounds from roots of Centaurea diffusa Lam.  

PubMed Central

An extract of roots of Centaurea diffusa (diffuse knapweed) yielded caryophyllene oxide and linoleic acid which were shown to be phytotoxic. Also isolated were germacrene B, a previously-known phytotoxin as well as the inactive polyene aplotaxene. A combination of these compounds, if transferred to the soil, could be one factor in the invasive behavior of this weed. Contrary to a literature report, 8-hydroxyquinoline was not detected in root exudates of in vitro grown C. diffusa nor could it be identified in the root extract. However, a recent report from a different group maintains that 8-hydroxyquinoline can be released from roots of C. diffusa following a diurnal rhythm. PMID:19568334

Quintana, Naira; El Kassis, Elie G; Stermitz, Frank R

2009-01-01

264

Interactive Event Search Through Transfer Antony Lam,1  

E-print Network

subjective (e.g. what the user thinks are "nice basketball shots"), there would be no way to train a system in mind and return refined results. If the user is still dissatisfied, then another iteration of user

Chowdhury, Amit K. Roy

265

Ecology and Management of Diffuse Knapweed (Centaurea diffusa Lam.)  

Microsoft Academic Search

A close relative of spotted knapweed in the Asteraceae taxonomic family, diffuse knapweed is typically biennial, reproducing exclusively by seed. It forms a rosette with a central crown and tap root in the juvenile stage and a single upright stem one to three feet (0.3 to 0.9 m) tall with numerous spreading branches at maturity. White (occasionally purple) flowers are

Jim Jacobs; Sharlene Sing

266

Life Science Fall 2011 110901 Eric Lam (Imperial College, London)  

E-print Network

-FOXM1 axis in cancer and drug resistance" Host: Marek Los, IGEN 110908 Hal Hoffman (UCSD in Human Cancers" Host: Anders Rosén 110929 Mahvash Tavassoli "Active PKC signaling sensitizes cancer cells) " Thioredoxin systems in oxidative and endoplasmic reticulum stress" Host: Stefan Thor (MMM) 111027 Hendrik J

Zhao, Yuxiao

267

Hemostatic, antibacterial biopolymers from Acacia arabica (Lam.) Willd. and Moringa oleifera (Lam.) as potential wound dressing materials.  

PubMed

Acacia arabica and Moringa oleifera are credited with a number of medicinal properties. Traditionally gum of Acacia plant is used in the treatment of skin disorders to soothe skin rashes, soreness, inflammation and burns while Moringa seed extracts are known to have antibacterial activity. In the present study the potential of the polymeric component of aqueous extracts of gum acacia (GA) and the seeds of M. oleifera (MSP) in wound management was evaluated. The results revealed that both biopolymers were hemostatic and hasten blood coagulation. They showed shortening of activated partial thromboplastin time and prothrombin time and were non-cytotoxic in nature. Both showed antibacterial activity against organisms known to be involved in wound infections with MIC ranging from 500-600 microg mL(-1) for GA and 300-700 microg mL(-1) for MSP. They were biodegradable and exhibited water absorption capacity in the range of 415 to 935%. The hemostatic character coupled to these properties envisions their potential in preparation of dressings for bleeding and profusely exuding wounds. The biopolymers have been further analysed for their composition by Gas chromatography. PMID:24266104

Bhatnagar, Monica; Parwani, Laxmi; Sharma, Vinay; Ganguli, Jhuma; Bhatnagar, Ashish

2013-10-01

268

Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine.  

PubMed

In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, and Microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover, antileishmanial and cytotoxicity activity of B. vulgaris and berberine against promastigotes of Leishmania major and Leishmania tropica were evaluated by colorimetric MTT assay. The findings indicated that the various extracts of B. vulgaris particularly berberine showed high potential antidermatophytic against pathogenic dermatophytes tested with MIC values varying from 0.125 to >4?mg/mL. The results revealed that B. vulgaris extracts as well as berberine were effective in inhibiting L. major and L. tropica promastigotes growth in a dose-dependent manner with IC50 (50% inhibitory concentration) values varying from 2.1 to 26.6? ? g/mL. Moreover, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages with CC50 (cytotoxicity concentration for 50% of cells) values varying from 27.3 to 362.6? ? g/mL. Results of this investigation were the first step in the search for new antidermatophytic and antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in animal models as well as volunteer human subjects. PMID:24977052

Mahmoudvand, Hossein; Ayatollahi Mousavi, Seyyed Amin; Sepahvand, Asghar; Sharififar, Fariba; Ezatpour, Behrouz; Gorohi, Fatemeh; Saedi Dezaki, Ebrahim; Jahanbakhsh, Sareh

2014-01-01

269

Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids  

Microsoft Academic Search

Extracts obtained from the roots of Berberidaceae species have been used in Eastern and Bulgarian folk medicine in rheumatic and other chronic inflammatory disorders. The investigations of the chemical composition and immunological properties show that their activity is mainly due to the alkaloid constituents. In the present study the anti-inflammatory properties of total ethanol extract (TEE), three alkaloid fractions, a

Nina Ivanovska; Stefan Philipov

1996-01-01

270

Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine  

PubMed Central

In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, and Microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover, antileishmanial and cytotoxicity activity of B. vulgaris and berberine against promastigotes of Leishmania major and Leishmania tropica were evaluated by colorimetric MTT assay. The findings indicated that the various extracts of B. vulgaris particularly berberine showed high potential antidermatophytic against pathogenic dermatophytes tested with MIC values varying from 0.125 to >4?mg/mL. The results revealed that B. vulgaris extracts as well as berberine were effective in inhibiting L. major and L. tropica promastigotes growth in a dose-dependent manner with IC50 (50% inhibitory concentration) values varying from 2.1 to 26.6??g/mL. Moreover, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages with CC50 (cytotoxicity concentration for 50% of cells) values varying from 27.3 to 362.6??g/mL. Results of this investigation were the first step in the search for new antidermatophytic and antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in animal models as well as volunteer human subjects. PMID:24977052

Mahmoudvand, Hossein; Ayatollahi Mousavi, Seyyed Amin; Sepahvand, Asghar; Sharififar, Fariba; Ezatpour, Behrouz; Gorohi, Fatemeh; Saedi Dezaki, Ebrahim; Jahanbakhsh, Sareh

2014-01-01

271

7 CFR 301.38-2 - Regulated articles.  

Code of Federal Regulations, 2014 CFR

...propagation from the following rust-resistant Berberis species and varieties. B. aggregata... M. piperiana M. pumila M. repens M. ×media ‘Charity’ M. ...rust-susceptible species and varieties of the genera Berberis , Mahoberberis , and Mahonia...

2014-01-01

272

7 CFR 301.38-2 - Regulated articles.  

Code of Federal Regulations, 2011 CFR

...propagation from the following rust-resistant Berberis species and varieties. B. aggregata...Hartman' M. piperiana M. pumila M. repens M. ×media 'Charity' M. ...rust-susceptible species and varieties of the genera Berberis , Mahoberberis , and Mahonia ,...

2011-01-01

273

7 CFR 301.38-2 - Regulated articles.  

Code of Federal Regulations, 2012 CFR

...propagation from the following rust-resistant Berberis species and varieties. B. aggregata...Hartman' M. piperiana M. pumila M. repens M. ×media 'Charity' M. ...rust-susceptible species and varieties of the genera Berberis , Mahoberberis , and Mahonia ,...

2012-01-01

274

7 CFR 301.38-2 - Regulated articles.  

Code of Federal Regulations, 2013 CFR

...propagation from the following rust-resistant Berberis species and varieties. B. aggregata...Hartman' M. piperiana M. pumila M. repens M. ×media 'Charity' M. ...rust-susceptible species and varieties of the genera Berberis , Mahoberberis , and Mahonia ,...

2013-01-01

275

[The use of barberry berries in human nutrition].  

PubMed

The contents of carbohydrates, organic acids, some vitamin, poliphenolic compounds, pectin tannin, mineral elements, in berberis vulgaris are presented. Describle utilize berberis in traditional medicine and dietetica are also outlined. PMID:12968305

Pozniakovski?, V M; Golub, O V; Popova, D G; Kovalevskaia, I N

2003-01-01

276

Biological Invasions 1: 189201, 1999. 1999 Kluwer Academic Publishers. Printed in the Netherlands.  

E-print Network

of Japanese barberry was encouraged as an ornamental shrub replacing the common barberry (Berberis vulgaris: the common barberry (Berberis vulgaris L.) and Japanese barberry. These are closely related species in the Netherlands. The invasion ecology of Japanese barberry (Berberis thunbergii) in the New England landscape John

Silander Jr., John A.

277

Effect of Dietary Dried Berberis Vulgaris Fruit and Enzyme on Some Blood Parameters of Laying Hens Fed Wheat-Soybean Based Diets  

Microsoft Academic Search

2 Abstract: A study was conducted to evaluate dried berberry fruit (DBF) and enzyme on some blood parameters of laying hens. In a 5 * 2 completely randomized block design with factorial arrangement and 4 blocks as replicate, 5 levels of DBF (0.0, 0.5, 1.0, 1.5, and 2%) and 2 levels of NSP-degrading enzyme (0.0, and 0.05%) in wheat-soybean based

2006-01-01

278

Clinical role of a fixed combination of standardized Berberis aristata and Silybum marianum extracts in diabetic and hypercholesterolemic patients intolerant to statins  

PubMed Central

Background Statin intolerance is a medical condition often leading patients to nonadherence to the prescribed therapy or to a relevant reduction of the statin dosage. Both situations determine a totally or partially uncontrolled lipid profile, and these conditions unquestionably increase the risk of cardiovascular events. Methods We enrolled hypercholesterolemic, type 2 diabetic patients complaining of intolerance to statins. Some of them had reduced the statin dose ‘until the disappearance of symptoms’; others had opted for treatment with ezetimibe; and yet others were not undergoing any treatment at all. All patients of the three groups were then given a fixed combination of berberine and silymarin (Berberol®), known from previous papers to be able to control both lipidic and glycemic profiles. Results The tested product both as a single therapy and as add-on therapy to low-dose statin or to ezetimibe reduced triglycerides, low-density lipoprotein cholesterol, fasting blood glucose, and glycosylated hemoglobin in a significant manner without inducing toxicity conditions that might be somehow ascribed to a statin-intolerant condition. Conclusion Our study demonstrates that use of Berberol®, administered as a single or add-on therapy in statin-intolerant subjects affected by diabetes and hypercholesterolemia is a safe and effective tool capable of improving the patients’ lipidic and glycemic profiles. PMID:25678808

Di Pierro, Francesco; Bellone, Iaele; Rapacioli, Giuliana; Putignano, Pietro

2015-01-01

279

Analysis of isoquinoline alkaloids in medicinal plants by capillary electrophoresis-mass spectrometry.  

PubMed

The technique of capillary electrophoresis - mass spectrometry (CE-MS) was applied for determination of isoquinoline alkaloids in crude methanolic extracts of medicinal plants. For the CE separations ammonium formate buffer solutions (70 or 100 mM, pH 3.0 or 4.0) containing 10% methanol or 20-60% acetonitrile as additives were used. The applied voltage was 25 kV, the thermostating temperature was kept constant at 25 degrees C. Coupling with the mass spectrometer was performed via an atmospherical pressure ionization (API) interface and the electrospray ionization technique (ESI). As sheath liquid 5 mM formic acid in acetonitrile at a flow rate of 3 microL/min was used. The spray voltage was 4.5 kV and the temperature of the heated capillary was chosen to be 200 degrees C. Detection in the positive ionization mode resulted in mass spectra showing either the molecular ions [M]+ or the protonated molecular ions [M+H]+. The presented method allows detection and identification of isoquinoline alkaloids in crude methanolic extracts of medicinal plants as Eschscholzia californica CHAM. (Papaveraceae), Hydrastis canadensis L. (Ranunculaceae), Berberis vulgaris L. (Berberidaceae), Jateorhiza palmata (LAM.) MIERS (Menispermaceae) and Chelidonium majus L. (Papaveraceae). PMID:9870408

Sturm, S; Stuppner, H

1998-11-01

280

Using Soot as a Program Optimizer Patrick Lam (plam@sable.mcgill.ca)  

E-print Network

, and unused local elimination. In order to optimize the Hello example from the previous tutorial, we issue the command: > java soot.Main -O Hello Transforming Hello... Soot will then leave a new, improved Hello. Soot does, however, eliminate unused locals. Try adding an unused local to Hello and giving

Verbrugge, Clark

281

Using Soot as a Program Optimizer Patrick Lam (plam@sable.mcgill.ca)  

E-print Network

, and unused local elimination. In order to optimize the Hello example from the previous tutorial, we issue the command: > java soot.Main ­O Hello Transforming Hello... Soot will then leave a new, improved Hello. Soot does, however, eliminate unused locals. Try adding an unused local to Hello and giving

Verbrugge, Clark

282

UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kevin Petersen, On Tak Lam, Zhejian Zhang  

E-print Network

. With this consumption, there is the opportunity to remanufacture the used cartridges and reduce the carbon footprint figures in regards to the waste and the reduction the carbon footprint are not available due will on reduce the costs of obtaining cartridges from 10%-30% while also reducing the carbon footprint

283

78 FR 3319 - Amendments to Existing Validated End User Authorizations: Advanced Micro Devices China, Inc., Lam...  

Federal Register 2010, 2011, 2012, 2013, 2014

...the Export Administration Regulations (EAR) to revise the existing Authorization Validated...amends Supplement No. 7 to part 748 of the EAR to update VEU Advanced Micro Devices China...specific eligible items. BIS also updates the EAR to amend the addresses and lists of...

2013-01-16

284

Identification, distribution, and control of triasulfuron-resistant Italian ryegrass (Lolium multiflorum Lam.) in Central Texas  

E-print Network

et al. 1994). The first report of herbicide resistance was that of triazine resistant common groundsel (Senecio vulgaris L. ) in 1970 (Heap 1997; Holt and Lebaron 1990; Ryan 1970). Since that time, over 150 herbicide resistant weed species...

Tucker, Kevin Patrick

2002-01-01

285

Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir.  

PubMed Central

This report describes in vitro micropropagation of Boscia senegalensis, so-called famine foods, that helped the people in Darfur and Kordofan, Sudan survive during the 1984–1985 famine. Four types of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1–5 mg/L 6-benzyladenine (BA). The highest number of shoots per explant (14.3±0.9) was achieved on MS medium supplemented with 3 mg/L BA, while the highest shoot length [(3.5±0.4) cm] was obtained with 1 mg/L BA. The shoot cluster, when subcultured to its same medium, significantly increased the rate of shoot multiplication by the end of the third subculture. The maximum mean number of shoots per explant (86.5±3.6) was produced after three multiplication cycles on 3 mg/L BA-supplemented medium. In vitro induced shoots were excised and rooted on half strength MS medium fortified with 0.25 mg/L indole-3-butyric acid (IBA) to obtain complete plantlets. B. senegalensis-regenerated plantlets obtained in vitro for the first time, were hardened and 95% survived under greenhouse conditions. PMID:21462387

Khalafalla, Mutasim M.; Daffalla, Hussien M.; Abdellatef, Eltayb; Agabna, Elsadig; El-Shemy, Hany A.

2011-01-01

286

Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir  

Microsoft Academic Search

This report describes in vitro micropropagation of Boscia senegalensis, so-called famine foods, that helped the people in Darfur and Kordofan, Sudan survive during the 1984–1985 famine. Four types\\u000a of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1–5\\u000a mg\\/L 6-benzyladenine (BA). The highest number of shoots per explant (14.3±0.9) was achieved

Mutasim M. Khalafalla; Hussien M. Daffalla; Eltayb Abdellatef; Elsadig Agabna; Hany A. El-Shemy

2011-01-01

287

Secure Mobile Code Execution Service Lap-chung Lam Yang Yu Tzi-cker Chiueh  

E-print Network

- parent browser plug-in or DLL, a new document viewer in- stalled on demand, an explicitly downloaded, modifying configurations or registry en- tries, sending emails, or installing back-door programs in the home

Chiueh, Tzi-cker

288

Homage to Manjusri (Byang-chub-lam-gyi-rim-pahi-nams-len) (trans.)  

E-print Network

fulfils the hopes of countless beings, Thou whose Mind accurately perceives all that can be known; Lord of the Sakyas, I do homage to Thee! Best of the spiritual sons of the incomparable Master, \\Vho bore the burden of the Buddha's universal mission... not know how to cut away its root. So one must be aware of the bondage of this same Samsara And hold to a regretful renunciation of the world. I, the seer, have practised thus: May you, who seek deliverance, do the same! THE PATH FOR "GREAT SEEKERS" I...

Lhalungpa, Lobsang

1968-02-29

289

Diffuse knapweed ( Centaurea diffusa Lam.) seedling emergence and establishment in a Colorado grassland  

Microsoft Academic Search

Knapweeds (Centaurea spp.) are damaging invaders of grasslands and other North American rangelands. A field study was conducted to determine conditions\\u000a that promote diffuse knapweed (C. diffusa) emergence and establishment in a native Colorado grassland (North America). Knapweed was planted in native grassland under\\u000a treatments with different opening sizes, levels of competition, knapweed seed burial and season of seeding. There was

Paul J. Meiman; Edward F. Redente; Mark W. Paschke

290

Diffuse knapweed ( Centaurea diffusa Lam.) seedling emergence and establishment in a Colorado grassland  

Microsoft Academic Search

Knapweeds (Centaurea spp.) are damaging invaders of grasslands and other North American rangelands. A field study was conducted to determine conditions\\u000a that promote diffuse knapweed (C. diffusa) emergence and establishment in a native Colorado grassland (North America). Knapweed was planted in native grassland under\\u000a treatments with different opening sizes, levels of competition, knapweed seed burial and season of seeding. There was

Paul J. Meiman; Edward F. Redente; Mark W. Paschke

2009-01-01

291

Quality of cooked ground buffalo meat treated with the crude extracts of Moringa oleifera (Lam.) leaves.  

PubMed

The study was conducted to evaluate the physico-chemical, microbial and organoleptic qualities of cooked ground buffalo meat (GBM), treated with, 1, 1.5 and 2% levels of aqueous solution of crude extract of drumstick (Moringa oleifera) leaves. The meat samples treated with 1.5% crude extract of drumstick leaves significantly (P??0.05) difference was observed in juiciness, tenderness and overall acceptability scores between the treated meat samples. PMID:23572848

Hazra, Suchandra; Biswas, Subhasish; Bhattacharyya, Debasish; Das, Sudip Kumar; Khan, Anupam

2012-04-01

292

Immunomodulatory effect of Moringa oleifera Lam. extract on cyclophosphamide induced toxicity in mice.  

PubMed

Immunomodulatory effect of ethanolic extract (50%) of M. oleifera leaves (MOE) has been studied in normal and immunosuppressed mice models. Different doses of MOE i.e. 125, 250 and 500 mg/kg body weight of mice were administered orally for 15 days. Cyclophosphamide at a dose of 30 mg/kg body weight was administered orally for the next 3 days. On day 16 and 19, hematological parameters like white blood cell (WBC) count, red blood cell (RBC) count, haemoglobin level (Hb), percent neutrophils and organ weight were recorded. Effect of MOE on phagocytic activity of mice macrophages was determined by carbon clearance test. MOE showed significant dose dependent increase in WBC, percent neutrophils, weight of thymus and spleen along with phagocytic index in normal and immunosuppressed mice. The results indicate that MOE significantly reduced cyclophosphamide induced immunosuppression by stimulating both cellular and humoral immunity. PMID:21117458

Gupta, Anamika; Gautam, Manish K; Singh, Rahul K; Kumar, M Vijay; Rao, Ch V; Goel, R K; Anupurba, Shampa

2010-11-01

293

Immunomodulatory effects of alcbholic and hydroalcoholic extracts of Moringa olifera Lam leaves.  

PubMed

Effects of 50, 100 and 200 mg/kg body weight of the alcoholic and hydro-alcoholic extract of leaves of M. olifera were studied on various immune paradigms like delayed type hypersensitivity reaction using SRBC as an antigen, determination of antibody titer, neutrophil adhesion test as an indicator for neutrophil index, total leucocyte count in cyclophosphamide induced immunosuppressed animals and carbon clearance assay as a measure of phagocytic activity. Hydro-alcoholic extract of M. olifera substantially enhanced cellular immune response, humoral immune response, neutrophil index and phagoctic activity in doses of 100 and 200 mg/kg body weight. The ethanolic extract (200 mg/kg body weight) was efficient in improving immune response. The results suggest that M. olifera has a significant role to play as an immune stimulator. PMID:22611915

Banji, Otilia J F; Banji, David; Kavitha, R

2012-04-01

294

Isolation and characterization of dipeptide derivative and phytosterol from Capparis tomentosa Lam  

Microsoft Academic Search

In this study, chemical investigation of the constituents of the roots of Capparis tomentosa was carried out. C. tomentosa is a medicinal plant used extensively for the treatment of various diseases. It is also known to be poisonous. The crude petroleum ether extract of the roots of this plant was subjected to column chromatography for separation. Progress of separation was

O. Akoto; I. V. Oppong; I. Addae-Mensah; Reiner Waibel; Hans Achenbach

2008-01-01

295

75 FR 76952 - Grant of Authority for Subzone Status; Lam Research Corporation (Wafer Fabrication Equipment...  

Federal Register 2010, 2011, 2012, 2013, 2014

...interest; Whereas, the City of San Jose, California, grantee of Foreign-Trade...Fremont, Newark, and Livermore, California, (FTZ Docket 36- 2010, filed...Fremont, Newark, and Livermore, California (Subzone 18F), as...

2010-12-10

296

Does leaf manipulation affect leaf appearance in italian ryegrass (Lolium multiflorum Lam.)  

Technology Transfer Automated Retrieval System (TEKTRAN)

Mechanical stimuli such as rubbing, shaking, or flexing plants can alter their growth rates and morphologies. Plant response to mechanical stress can result in delayed plant growth, reduced leaf size, shorten and thicken stems, and reduced yields. Repeated measurements, such as leaf counting or me...

297

Integrating LMSs in the Educational Process: Greek Teachers' Initial Perceptions about LAMS  

ERIC Educational Resources Information Center

E-learning with the use of Learning Management Systems, has been increasingly adopted in Primary, Secondary and Higher Education with the expectation to increase students' motivation and infuse activity-centred learning strategies with various educational benefits. This study has investigated the initial perceptions of Greek teachers about the…

Papadakis, Spyros; Dovros, Nikos; Paschalis, Giorgos; Rossiou, Eleni

2012-01-01

298

Integration and test activities for the SUMIRE prime focus spectrograph at LAM  

NASA Astrophysics Data System (ADS)

The Prime Focus Spectrograph (PFS) of the Subaru Measurement of Images and Redshifts (SuMIRe) project for Subaru telescope consists in four identical spectrographs feed by 600 fibers each. Each spectrograph is composed by an optical entrance unit that creates a collimated beam and distributes the light to three channels, two visible and one near infrared. We present here the integration process of the first spectrograph channel. The verification requirements, the specific integration requirements and the product tree are the main drivers from the top plan for the Assembly Integration and Test (AIT) development process. We then present the AIT flow-down, the details for the AIT processes as well as opto-mechanical alignment procedures and tests setup. In parallel, we are developing and validating dedicated tools to secure and facilitate the AIT activities, as we have to assemble eight visible cameras, integrate and align four fiber slits, integrate and align the components of four spectrographs.

Madec, F.; Jaquet, Marc; Pascal, Sandrine; Bozier, A.; Le Mignant, David; Vives, S.; Ferrand, D.; Pegot-Ogier, T.; Arthaud, G.; Golebiowski, M.; Sugai, H.; Tamura, N.; Gunn, J.; Smee, S.; Oliveira, L.

2014-07-01

299

Design of a VIA Based Communication Protocol for LAM\\/MPI Suite  

Microsoft Academic Search

The increasing use of System Area Network (SAN) de- mands efficient communication to benefit of SAN features through a direct access to network resources and avoiding kernel intervention in communication path. Recently, a consortium composed by Microsoft, Compaq and Intel authored a new standard, the Virtual Interface Ar- chitecture (VIA), designed to reduce software overhead in data transfers. This paper

Massimo Bertozzi; Marco Panella; Monica Reggiani

2001-01-01

300

Antiplasmodial activity of selected Sudanese medicinal plants with emphasis on Maytenus senegalensis (Lam.) Exell.  

PubMed

The antiplasmodial activity of plant extracts related to four families was tested on chloroquine sensitive strain 3D7 and chloroquine resistant strain Dd2 of Plasmodium falciparum. The methanolic extract of Harrisonia abyssinica (Simaroubaceae) inhibited Dd2 with IC50 value of 4.7 microg/ml, while in 3D7, the IC50 value was 10 microg/ml. Most of the plants from the family Meliaceae showed highly potent antiplasmodial activity against the two tested strains. Khaya senegalensis, Azadirachta indica and Trichilia emetica showed IC50 values less than 5 microg/ml. The methanolic extract of Annona squamosa (Annonaceae) leaves showed high antiplasmodial activity with IC50 values of 2 and 30 microg/ml on 3D7 and Dd2, respectively. While stem bark showed moderate activity with IC50 values of 8.5 and 120 microg/ml on Dd2. Maytenus senegalensis (Celastraceae) possessed IC50 values of 3.9 on 3D7, 10 microg/ml on Dd2 and had no effect on lymphocyte proliferation even at the highest tested concentration; the IC50 was greater than 100 microg/ml. Liquid-liquid separation of the methanolic extract of M. senegalensis revealed that the dichloromethane extract possessed an IC50 value of only 2.1 microg/ml. Column fractionation of dichloromethane extract gave four fractions and fraction two showed an IC50 value of 0.5 microg/ml. Preliminary phytochemical analysis of dichloromethane fraction revealed terpenoids and traces of phenolic principles but no alkaloid, tannins or flavonoids were detected. PMID:10363837

El Tahir, A; Satti, G M; Khalid, S A

1999-03-01

301

Antitrypanosomal Property of Some Extracts of Different Parts of Moringa oleifera, Lam  

Microsoft Academic Search

Different extracts of Moringa oleifera parts were evaluated for their anti-trypanosomal activity and broad phytochemical classes. Alkaloids, resins and saponins were detected. In vitro, the petroleum ether extract of the root bark, chloroform extract of the stem bark, methanol extracts of the stem and the aqueous extracts of all parts were active at 4 and 2 mg\\/ ml doses. The

Sunday E. Atawodi; Hassana Shehu

302

Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds  

Microsoft Academic Search

Seeds of the tropical tree Moringa oleifera contain small storage proteins able to flocculate particles in suspension in water. The cDNA encoding one of these flocculent proteins, MO2.1, was cloned and the recombinant protein was expressed in Escherichia coli. The flocculent activity of the purified recombinant MO2.1 was assayed on clays and bacteria using light and confocal microscopy and GFP-overexpressing

M. Broin; C. Santaella; S. Cuine; K. Kokou; G. Peltier; T. Joët

2002-01-01

303

Quality of cooked ground buffalo meat treated with the crude extracts of Moringa oleifera (Lam.) leaves  

Microsoft Academic Search

The study was conducted to evaluate the physico-chemical, microbial and organoleptic qualities of cooked ground buffalo meat\\u000a (GBM), treated with, 1, 1.5 and 2% levels of aqueous solution of crude extract of drumstick (Moringa oleifera) leaves. The\\u000a meat samples treated with 1.5% crude extract of drumstick leaves significantly (P?

Suchandra Hazra; Subhasish Biswas; Debasish Bhattacharyya; Sudip Kumar Das; Anupam Khan

304

Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds.  

PubMed

Seeds of the tropical tree Moringa oleifera contain small storage proteins able to flocculate particles in suspension in water. The cDNA encoding one of these flocculent proteins, MO(2.1), was cloned and the recombinant protein was expressed in Escherichia coli. The flocculent activity of the purified recombinant MO(2.1)was assayed on clays and bacteria using light and confocal microscopy and GFP-overexpressing bacteria. We show that MO(2.1)is able to aggregate montmorillonite clay particles as well as gram-positive and gram-negative bacteria. We discuss the use of recombinant proteins to study flocculating properties and improve water purification processes. PMID:12382051

Broin, M; Santaella, C; Cuine, S; Kokou, K; Peltier, G; Joët, T

2002-10-01

305

Genetic diversity and population structure of Butea monosperma (Lam.) Taub.- a potential medicinal legume tree.  

PubMed

Three molecular marker systems, Random Amplified Polymorphic DNA (RAPD), Inter-Simple Sequence Repeats (ISSR) and Sequence-Related Amplified Polymorphism (SRAP) were employed to investigate the genetic structure and diversity among the 14 natural populations of Butea monosperma collected from different geographical regions of India. Detected by 17 RAPD, 15 ISSR and 11 SRAP primer combinations, the proportions of polymorphic bands were 84.2 %, 77.2 % and 91.9 %, respectively, and the mean Nei's genetic distances among the populations were 0.13, 0.10 and 0.13, respectively. Partitioning of genetic variability by Analysis of molecular variance (AMOVA) revealed that the high genetic diversity was distributed within the populations. AMOVA also revealed that the coefficient of gene differentiation among populations based on FST was very high irrespective of markers used. The overall gene flow among populations (Nm) was very low. Cophenetic correlation coefficients of Nei's distance values and clustering pattern by Mental test were statistically significant for all three marker systems used but poor fit for ISSR data than for RAPD, SRAP and combined data set of all three markers. For all markers, a high similarity in dendrogram topologies was obtained, although some differences were observed with ISSR. The dendrogram obtained by RAPD, SRAP and combined data set of all three markers reflect relationship of most of the populations according to their geographic distribution. PMID:24431507

Vashishtha, Amit; Jehan, Tabassum; Lakhanpaul, Suman

2013-07-01

306

Feeding preferences of the collembolan Onychiurus sinensis for fungi colonizing holm oak litter (Quercus rotundifolia Lam.)  

E-print Network

of our test fungi, but not on the Basidiomycete S41 and on Trichoderma polysporum. Collembola / feeding reproduire, à l'exception du Basidiomycète S41 et de Trichoderma polysporum. Collemboles/ préférences

Paris-Sud XI, Université de

307

Genome Sequence of the Sulfate-Reducing Bacterium Desulfotomaculum hydrothermale Lam5T  

E-print Network

and thermal ecosystems (1). Evi- dence of their presence in terrestrial geothermal hot springs has been a Tunisian hot spring. The genome is composed of 2.7 Mb, with a G C content of 49.48%, and it contains 2 draft genome sequence of a Desulfotomaculum spe- cies isolated from a terrestrial hot spring in Tunisia

Boyer, Edmond

308

Establishment of bermudagrass (Cynodon dactylon (L.) Pers.) seeded with annual ryegrass (Lolium multiflorum Lam.  

E-print Network

root material. Accumulation of these sub- stances would be difficult in view of their apparent solubility in water. Results from this study indicate that factors other than a natural germination or growth inhibitor in ryegrass are responsible... pounds per acre of sodium TCA(right) severely reduced the stand of all plants 1 month follow- :. ing treatment, but 0. 5 pounds per acre of paraquat (left) permitted stands of green vegetation similar to either the mowed or the untreated plots...

Bowmer, William Jackson

1967-01-01

309

In vitro callus induction and estimation of plumbagin content from Plumbago auriculata Lam.  

PubMed

The medicinal plant Plumbago contains a very potent secondary metabolite, plumbagin having many therapeutic properties. Callus culture was induced using explants, leaf, stem and shoot apex, from P. auriculata. Murashige and Skoog media fortified with various growth hormones like NAA, IAA, IBA and 2, 4-D individually and in various combinations were checked for callus induction. Among the growth hormones used, 1 mg/L 2, 4-D showed best callusing. The hormonal combinations of 1 mg/L IAA and 1.5 mg/L NAA in the media exhibited best callus induction using stem internode as an explant. Plumbagin content from root, stem, leaf and callus was analyzed by using thin layer chromatographic technique. The callus derived from stem showed comparable plumbagin content to the in vivo plant parts. Quantitative spectrophotometric analysis of plumbagin from plant samples and callus indicated that plumbagin content was maximum in roots which was followed by callus, stem and leaf samples respectively. Generation of in vitro sources for p!umbagin, for therapeutic applications will serve as a continuous supply and will contribute to preserve the natural plant recourses. PMID:25434108

Deshpande, Jyoti; Labade, Dinesh; Shankar, Kalaiselvi; Kata, Navin; Chaudhari, Manoj; Wani, Minal; Khetmalas, Madhukar

2014-11-01

310

In vitro callus induction and estimation of plumbagin content from Plumbago auriculata Lam.  

PubMed

The medicinal plant Plumbago contains a very potent secondary metabolite, plumbagin having many therapeutic properties. Callus culture was induced using explants, leaf, stem and shoot apex, from P. auriculata. Murashige and Skoog media fortified with various growth hormones like NAA, IAA, IBA and 2, 4-D individually and in various combinations were checked for callus induction. Among the growth hormones used, 1 mg/L 2, 4-D showed best callusing. The hormonal combinations of 1 mg/L IAA and 1.5 mg/L NAA in the media exhibited best callus induction using stem internode as an explant. Plumbagin content from root, stem, leaf and callus was analyzed by using thin layer chromatographic technique. The callus derived from stem showed comparable plumbagin content to the in vivo plant parts. Quantitative spectrophotometric analysis of plumbagin from plant samples and callus indicated that plumbagin content was maximum in roots which was followed by callus, stem and leaf samples respectively. Generation of in vitro sources for p!umbagin, for therapeutic applications will serve as a continuous supply and will contribute to preserve the natural plant recourses. PMID:25507706

Deshpande, Jyoti; Labade, Dinesh; Shankar, Kalaiselvi; Kata, Navin; Chaudhari, Manoj; Wani, Minal; Khetmalas, Madhukar

2014-11-01

311

Leucanthemum vulgare lam. germination, growth and mycorrhizal symbiosis under crude oil contamination.  

PubMed

Oil contamination of soil limits plants' access to water and nutrients. Leucanthemum vulgare colonized by mycorrhizae could provide an effective tool in remedying oil contamination. Seeds of L. vulgare were planted in pots containing soil mixed with petroleum at 0, 2.5, 5, 7.5, and 10% w/w and propagules of mycorrhizal fungi. Plants were grown under ambient conditions for 16 weeks. Seed germination data were collected weekly for three weeks. Mycorrhizal percentage, spore counts, length and weight of roots and shoots were determined after harvesting. Results showed significant differences in seed germination rates between oil-treated, mycorrhizal and non-mycorrhizal plants. The overall germination rate was greater at 7.5% w/w crude oil contamination (p = 0.05) in mycorrhizal and non-mycorrhizal pots with significant differences between their respective Root:Shoot ratios (both length and weight). Results of this research showed L. vulgare could be germinated and grown in crude oil contaminated soils and could be used to augment plant establishment as part of phytoremediation practices. PMID:24933896

Noori, Azam Sadat; Maivan, Hassan Zare; Alaie, Ebrahim

2014-01-01

312

Triterpenes and Steroids from Euphorbia denticulata Lam. With Anti-Herpes Symplex Virus Activity  

PubMed Central

In this research, dried acetone:chloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3?,25-diol, cycloart-23E-ene-3?,25- diol, ergosta-8,24-dien-3-ol (obtusifoliol) and beta-sitosterol which were reported for the first time from this plant. The structure of these compounds was elucidated by NMR and mass spectroscopic methods. The MTS assay was used to determine the toxicity and antiviral activity of betulin and (3?,23E)-cycloarta-23-ene-3,25-diol. Betulin showed anti-HSV-1 activity with EC50 value of 84.37±0.02 ?g/mL, and toxicity on normal vero cells with CC50 value of 660.718±0.072 ?g/mL. (3?,23E)-Cycloarta-23-ene-3,25-diol showed antiviral effect with EC50 value of 86.63±0.03 ?g/mL, and toxicity with CC50 value of 1089.21±0.25 ?g/mL. The results revealed that these two compounds have the antiviral activity far below the CC50 value with selectivity index (CC50/EC50) values of 7.83, and 12.57, respectively. PMID:24523756

Shamsabadipour, Sara; Ghanadian, Mustafa; Saeedi, Hojjatollah; Rahimnejad, Mohammad Reza; Mohammadi-Kamalabadi, Marzieh; Ayatollahi, Seyed Majid; Salimzadeh, Loghman

2013-01-01

313

Vital technology as a human right By Arthur Lerner-Lam, Leonardo Seeber, and Robert Chen  

E-print Network

of fragile community and social structures. Millions will be at increased risk from disease and starvation. From hurricanes in the Caribbean to earthquakes in Southeast Asia to drought in Africa, we know enough

Qian, Ning

314

Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir.  

PubMed

This report describes in vitro micropropagation of Boscia senegalensis, so-called famine foods, that helped the people in Darfur and Kordofan, Sudan survive during the 1984-1985 famine. Four types of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1-5 mg/L 6-benzyladenine (BA). The highest number of shoots per explant (14.3±0.9) was achieved on MS medium supplemented with 3 mg/L BA, while the highest shoot length [(3.5±0.4) cm] was obtained with 1 mg/L BA. The shoot cluster, when subcultured to its same medium, significantly increased the rate of shoot multiplication by the end of the third subculture. The maximum mean number of shoots per explant (86.5±3.6) was produced after three multiplication cycles on 3 mg/L BA-supplemented medium. In vitro induced shoots were excised and rooted on half strength MS medium fortified with 0.25 mg/L indole-3-butyric acid (IBA) to obtain complete plantlets. B. senegalensis-regenerated plantlets obtained in vitro for the first time, were hardened and 95% survived under greenhouse conditions. PMID:21462387

Khalafalla, Mutasim M; Daffalla, Hussien M; Abdellatef, Eltayb; Agabna, Elsadig; El-Shemy, Hany A

2011-04-01

315

Antimicrobial and Antioxidant Activities of Bauhinia racemosa Lam. and Chemical Content  

PubMed Central

Methanol 70% extract of Bauhinia racemosa aerial parts was tested for antimicrobial activity against different bacterial and fungal strains and for antioxidant activity and also total content of polyphenols with phytochemical analysis of the extract was determined. The results have showed that the extract has a significant antimicrobial activity, it inhibited the growth of Bacillus subtilis and also it was highly active against Candida albicans suggesting that it can be used in the treatment of fungal infections. The extract has a good antioxidant activity, it has shown high values of the trolox equivalent antioxidant capacity and polyphenol content while it has shown a lower value of oxygen radical absorbance capacity. Phytochemical analysis has shown that it has interesting phytochemical bioconstituents, include flavonoids, tannins and others, and the deep phytochemical study results in the isolation of methyl gallate, gallic, kaempferol, quercetin, querection 3–O–?–rhamnoside, kaempferol 3–O–?–glucoside, myricetin–3–O–?– glucoside, querection–3–O–rutinoside (Rutin). All compounds were identified by different spectroscopic analyses (UV, 1H–NMR, 13C–NMR) and Mass Spectrometry (MS). PMID:25276210

Rashed, Khaled; Butnariu, Monica

2014-01-01

316

Growth of mycorrhized seedlings of Leucaena leucocephala (Lam.) de Wit. in a copper contaminated soil  

Microsoft Academic Search

Due to the low infectivity potential of arbuscular mycorrhizal fungi (AMF) in a mining area located at the State of Bahia, Northeastern Brazil, the effect of mycorrhization on the seedlings of Leucaena leucocephala was investigated, in order to use this species for revegetation of the area. Caatinga soils from both, natural (control) and mining impacted areas, were used to maintain

Cláudia Elizabete Lima Lins; Uided Maaze T. Cavalcante; Everardo V. S. B. Sampaio; Arminda Sacconi Messias; Leonor Costa Maia

2006-01-01

317

Touch and Run with Near Field Communication (NFC) Ben Dodson Hristo Bojinov Monica S. Lam  

E-print Network

ticketing systems, as well as being a generally pro- grammable platform. During a transaction, one party can of smartphones. The primary driver for the adoption of NFC on cell phones is contactless payments and ticket- ing phones presents an exciting opportunity for system and application designers, because not only can phones

Straight, Aaron

318

Antibacterial activity of bark extracts of Moringa oleifera Lam. against some selected bacteria.  

PubMed

The methanol, chloroform, ethyl acetate and aqueous bark extracts of Moringa oleifera were evaluated for their antibacterial activity against four bacteria viz. Staphylococcus aureus, Citrobacter freundii, Bacillus megaterium and Pseudomonas fluorescens using erythromycin as positive control. The activity was analyzed using paper disc diffusion method at different concentration of the extract. The study revealed that all the bark extracts irrespective of their types, in different concentrations inhibited growth of the test pathogens to varying degrees. Ethyl acetate extract showed maximum activity against all the bacterial strains followed in descending order by chloroform, methanol and aqueous extracts. The activity decreased with decrease in concentration of the extract. Staphylococcus aureus was found to be the most sensitive test organism to different extracts of Moringa oleifera. Looking to these results it may be concluded that M. oleifera may be a potential source for the treatment of different infections caused by the resistant microbes. PMID:25362592

Zaffer, Mudasser; Ahmad, Showkat; Sharma, Rajendra; Mahajan, Surabhi; Gupta, Ankur; Agnihotri, Rajneesh Kumar

2014-11-01

319

Bioremediation of Turbid Surface Water Using Seed Extract from the Moringa oleifera Lam. (Drumstick) Tree.  

PubMed

An indigenous water treatment method uses Moringa oleifera seeds in the form of a crude water-soluble extract in suspension, resulting in an effective natural clarification agent for highly turbid and untreated pathogenic surface water. Efficient reduction (80.0% to 99.5%) of high turbidity produces an aesthetically clear supernatant, concurrently accompanied by 90.00% to 99.99% (1 to 4 log) bacterial reduction. Application of this low-cost Moringa oleifera protocol is recommended for water treatment where rural and peri-urban people living in extreme poverty are presently drinking highly turbid and microbiologically contaminated water. Curr. Protoc. Microbiol. 33:1G.1-1G.8. © 2014 by John Wiley & Sons, Inc. PMID:24789599

Lea, Michael

2014-01-01

320

Antioxidant, antimicrobial and phytochemical variations in thirteen Moringa oleifera Lam. cultivars.  

PubMed

A study was undertaken to assess variation in antioxidant, antimicrobial and phytochemical properties of thirteen Moringa oleifera cultivars obtained from different locations across the globe. Standard antioxidant methods including the DPPH scavenging, ferric reducing power (FRAP) and ?-carotene-linoleic acid model were used to evaluate the activity. Variation in the antioxidant activity was observed, with TOT4951 from Thailand being the most active, with activity five times higher than that of ascorbic acid (reference compound). A different trend was observed for the activity in the FRAP and ?-carotene-linoleic acid assays. Antimicrobial activity was tested against Gram-positive (Staphylococcus aureus) and Gram-negative (Klebsiella pneumoniae) strains using the microdilution method. Acetone extracts of all cultivars exhibited good antibacterial activity against K. pneumoniae (MIC values of 0.78 mg/mL). The remaining extracts exhibited weak activity against the two microorganisms. For the antifungal activity, all the extracts exhibited low activity. Variations were observed in the total phenolic and flavonoid contents. Cultivars TOT5169 (Thailand) and SH (South Africa) exhibited highest amounts of total phenolic compounds while TOT5028 (Thailand) exhibited the lowest amounts of five times lower than the highest. The information offer an understanding on variations between cultivars from different geographical locations and is important in the search for antioxidant supplementation and anti-ageing products. PMID:25045889

Ndhlala, Ashwell R; Mulaudzi, Rofhiwa; Ncube, Bhekumthetho; Abdelgadir, Hafiz A; du Plooy, Christian P; Van Staden, Johannes

2014-01-01

321

Evaluation of cytotoxicity of Moringa oleifera Lam. callus and leaf extracts on Hela cells  

PubMed Central

Background: There are considerable attempts worldwide on herbal and traditional compounds to validate their use as anti-cancer drugs. Plants from Moringaceae family including Moringa oleifera possess several activities such as antitumor effect on tumor cell lines. In this study we sought to determine if callus and leaf extracts of M. oleifera possess any cytotoxicity. Materials and Methods: Ethanol-water (70-30) extracts of callus and leaf of M. oleifera were prepared by maceration method. The amount of phenolic compounds of the extracts was determined by Folin Ciocalteu method. The cytotoxicity of the extracts against Hela tumor cells was carried out using MTT assay. Briefly, cells were seeded in microplates and different concentrations of the extract were added. Cells were incubated for 48 h and their viability was evaluated by addition of tetrazolium salt solution. After 3 h medium was aspirated, dimethyl sulfoxide was added and absorbance was determined at 540 nm with an ELISA plate reader. Cytotoxicity was considered when more than 50% reduction on cell survival was observed. Results: Callus and leaf extracts of M. oleifera significantly decreased the viability of Hela cells in a concentration-dependent manner. However, leaf extract of M. oleifera were more potent than that of callus extract. Conclusion: As the content of phenolic compounds of leaf extract was higher than that of callus extract, it can be concluded that phenolic compounds are involved in the cytotoxicity of M. oleifera. PMID:25337524

Jafarain, Abbas; Asghari, Gholamreza; Ghassami, Erfaneh

2014-01-01

322

ROME: Routing On Metropolitan-scale Ethernet Chen Qian and Simon S. Lam  

E-print Network

and scalable to tens of thousands of switches and millions of end hosts. ROME is based upon a recently simulation performance results for ROME networks with up to 25,000 switches and 1.25 million hosts. I developed geographic routing protocol, greedy distance vector (GDV). Switches in ROME do not need any

Hayes, Jane E.

323

AMLIORATION DES PLANTES Utilisation du gne brown-midrib-3 pour l'am-  

E-print Network

. I. — Rétrocroise- ments et sélection généalogique Yves BARRIÈRE, André GALLAIS Henri BERTHET., 1971). Ces gènes affectent également la composition chimique de la lignine (Kuc & NELSON, 1964 ; GEE et

Paris-Sud XI, Université de

324

Clinical & Experimental Pathology Lamming, J Clin Exp Pathol 2012, S4  

E-print Network

aid in slowing the aging process and attenuating age-related diseases. In this review, we will discuss sources, including berries, peanuts, and red wine [1]. In 2003, the laboratories of Howitz KT et al. [2 from negative consequences of a high-fat diet, rather than ameliorating the underlying aging process

Sabatini, David M.

325

Seeding rate and seed size as management techniques for ryegrass (Lolium Multiflorum, Lam) in winter wheat  

E-print Network

yields at the same three wheat seed sizes, two wheat densities of 250 plants m-2 and 400 plants m-2, and three ryegrass densities of 0, 100 and 200 plants m-2. Small, bulk, and large wheat seed produced similar yields in both years: one season favorable...

Cook, Casey Lee

2005-08-29

326

Antimicrobial and Antioxidant Activities of Bauhinia racemosa Lam. and Chemical Content.  

PubMed

Methanol 70% extract of Bauhinia racemosa aerial parts was tested for antimicrobial activity against different bacterial and fungal strains and for antioxidant activity and also total content of polyphenols with phytochemical analysis of the extract was determined. The results have showed that the extract has a significant antimicrobial activity, it inhibited the growth of Bacillus subtilis and also it was highly active against Candida albicans suggesting that it can be used in the treatment of fungal infections. The extract has a good antioxidant activity, it has shown high values of the trolox equivalent antioxidant capacity and polyphenol content while it has shown a lower value of oxygen radical absorbance capacity. Phytochemical analysis has shown that it has interesting phytochemical bioconstituents, include flavonoids, tannins and others, and the deep phytochemical study results in the isolation of methyl gallate, gallic, kaempferol, quercetin, querection 3-O-?-rhamnoside, kaempferol 3-O-?-glucoside, myricetin-3-O-?- glucoside, querection-3-O-rutinoside (Rutin). All compounds were identified by different spectroscopic analyses (UV, (1)H-NMR, (13)C-NMR) and Mass Spectrometry (MS). PMID:25276210

Rashed, Khaled; Butnariu, Monica

2014-01-01

327

Antimicrobial, Antioxidant, and Wound Healing Properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC.  

PubMed Central

Microbial infections of various types of wounds are a challenge to the treatment of wounds and wound healing. The study was to investigate antimicrobial and antioxidant properties of methanol leaf and stem bark extracts of Kigelia africana and methanol leaf and root extracts of Strophanthus hispidus and also to determine wound healing properties of the extracts. The antimicrobial activities of the methanol extracts were determined against two Gram-positive and two Gram-negative bacteria and a fungus using agar diffusion and micro-dilution methods. The antioxidant activity was determined using 1,1-diphenyl-2-picryl–hydrazyl (DPPH) method. The influence of the extracts on rate of wound closure was investigated using the excision wound model and histopathological investigation of treated and untreated wound tissues performed. The MICs of leaf extract of K. africana against test organisms were 2.5–7.5?mg/mL and stem bark extract were 2.25–7.5?mg/mL. The leaf extract of S. hispidus had MIC range of 2.5–7.5?mg/mL and 2.5–10?mg/mL for root extract. The IC50 of leaf and stem bark extracts of K. africana were 56.9 and 13.7??g/mL, respectively and leaf and root of S. hispidus were 49.8 and 45.1??g/mL, respectively. K. africana extracts (7.5% w/w) showed significant (P < 0.05) wound contraction at day 7 with 72% of wound closure whiles significant (P < 0.05) wound contractions were observed on day 11 for stem bark of K. africana, leaf and root extracts of S. hispidus. Wound tissues treated with the extracts showed improved collagenation, re-epitheliazition and rapid granulation formation compared with untreated wound tissues. The extracts were found to contain alkaloids, saponins, tannins, flavonoids, carbohydrates, and sapogenetic glycosides. The HPLC finger-printing of the extracts were developed. The leaf, stem bark and root extracts of K. africana and S. hispidus exhibited antimicrobial, antioxidant, and enhanced wound healing properties and these may justify the medicinal uses of the plants for treatment of microbial infections and wounds. PMID:23662099

Agyare, Christian; Dwobeng, Anita Serwaa; Agyepong, Nicholas; Boakye, Yaw Duah; Mensah, Kwesi Boadu; Ayande, Patrick George; Adarkwa-Yiadom, Martin

2013-01-01

328

Light effects on leaf development and photosynthetic capacity of Hydrocotyle bonariensis Lam  

Microsoft Academic Search

Rates of net CO2 uptake were examined in developing leaves of Hydrocotyle bonariensis. Leaves that developed under high photosynthetically active radiation (48 mol m-2 day-1 PAR) were smaller, thicker, and reached maximum size sooner than did leaves that developed under low PAR (4.8 mol m-2 day-1). Maximum net CO2 uptake rates were reached after 5 to 6 days expansion for

David J. Longstreth; Terry L. Hartsock; Park S. Nobel

1981-01-01

329

State Arboretum of Virginia at Blandy Experimental Farm Alien Invasive Landscape Plants in Virginia  

E-print Network

calleryana Callery, Ornamental or Bradford Pear Quercus acutissima Sawtooth Oak Salix alba White Willow Ulmus pumila Siberian Elm Shrubs: Berberis thunbergii Japanese Barberry Buddleja davidii Butterfly Bush Cytisus

Acton, Scott

330

Molecular analysis of Iranian seedless barberries via SSR  

Microsoft Academic Search

Little is known about the genetic diversity of seedless barberry cultivars and its wild type relatives. Microsatellite markers next to the morphological traits were used to distinguish the relatedness of some 47 genotypes; forty-five Iranian genotypes including both seedless cultivars and wild types and two foreign genotypes: Berberis vulgaris and Berberis thunbergii. SSR primers were designed based on seven Mahonia

Mehdi Rezaei; Ali Ebadi; Stefanie Reim; Reza Fatahi; Ahmad Balandary; Naser Farrokhi; Magda-Viola Hanke

2011-01-01

331

Isolation and characterization of microsatellite markers in the invasive shrub Mahonia aquifolium (Berberidaceae) and their applicability in related species  

Microsoft Academic Search

Microsatellite loci were isolated from a Mahonia aquifolium cultivar. We describe the var- iability of 10 loci in invasive European and native North American M. aquifolium and their transspecies amplification in native Mahonia repens and Mahonia pinnata from North America and one species of the related genus Berberis ( Berberis vulgaris ), native to Europe. The markers should be useful

C. ROss; W. DURKA

2006-01-01

332

Genus Pimelea (Thymelaeaceae) in New Zealand 1. The taxonomic treatment of seven endemic, glabrous?leaved species  

Microsoft Academic Search

The nature and taxonomic history of the Australian and New Zealand shrub genus Pimelea are summarised, emphasising its manifestations in New Zealand. Five previously recognised glabrous?leaved Pimelea species are redescribed here and typified: P. longifolia, P. gnidia, P. buxifolia, P. traversii, and P. poppelwellii. Pimelea crosby?smithiana is merged, without any formal status, into the otherwise variable P. gnidia. Two new

Colin J. Burrows

2008-01-01

333

Proceedings of the 17th Central Hardwood Forest Conference GTR-NRS-P-78 (2011) 650 Japanese barberry (Berberis thunbergii) is classified as invasive in 20 states and four Canadian provinces. It is  

E-print Network

the following summer will be needed for eradication. Control of Japanese barberry had an effect on earthworm biomass (F = 6.86, d.f. = 2, P Earthworm biomass averaged 197 pounds per acre where barberry was controlled and 535 pounds per acre in dense barberry infestations. By comparison, earthworm biomass was 304

2011-01-01

334

Identification of eighteen Berberis species as alternate hosts of Puccinia striiformis f. sp. tritici and virulence variation in the pathogen isolates from natural infection of barberry plants in China  

Technology Transfer Automated Retrieval System (TEKTRAN)

The wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici, Pst) population in China has been reported to be a distinct genetic group with higher diversity than those in many other countries. Genetic recombination in the Pst population has been identified with molecular markers, but whethe...

335

Stereoselective copper-catalyzed Chan-Lam-Evans N-arylation of glucosamines with arylboronic acids at room temperature.  

PubMed

An efficient and practical N-arylation of glycosylamines with substituted aryl boronic acids has been established. Using Cu(OAc)2 and pyridine at room temperature under air atmosphere, the protocol proved to be general, and a variety of aryl N-glycosides have been prepared in good to excellent yields with exclusive ? selectivity. PMID:23928939

Bruneau, Alexandre; Brion, Jean-Daniel; Alami, Mouad; Messaoudi, Samir

2013-09-28

336

75 FR 29722 - Foreign-Trade Zone 18-San Jose, CA; Application for Subzone; Lam Research Corporation (Wafer...  

Federal Register 2010, 2011, 2012, 2013, 2014

...fittings, gaskets, washers, seals, valves, taps, bearings...non-adhesive sheets, tempered glass, laboratory glassware, fittings and components of metal (precious, ferrous, and...precious stone articles, base metal tools, pumps, fans,...

2010-05-27

337

UHF RFID TAG CHARACTERIZATION: OVERVIEW AND STATE-OF-THE-ART Pavel Nikitin, KVS Rao, Sander Lam  

E-print Network

power link budget diagram of a monostatic RFID system, where the tag is located at a distance d away that the diagram in Figure 1 is valid for monostatic system, far field scenarios (when readers/tags are far from

Hochberg, Michael

338

Impedance Matching Concepts in RFID Transponder Design K. V. S. Rao, Pavel V. Nikitin, Sander F. Lam  

E-print Network

Impedance Matching Concepts in RFID Transponder Design K. V. S. Rao, Pavel V. Nikitin, Sander F frequency identification (RFID) transpon- ders, which are powered by the incoming RF energy and consist RFID transponder design, an Intellitag ID card with embedded folded mean- der antenna operating in 915

Washington at Seattle, University of

339

Evaluating the last remnants of Butea monosperma (Lam.) Kuntze Forest for their in situ conservation: a case study.  

PubMed

This paper provides a suitable framework for consolidating knowledge and providing a useful starting point to undertake conservation of the last remnants of Butea monosperma forest. A survey was conducted to assess the status of a reportedly unexplored B. monosperma forest in a narrow strip of land along the northeastern region of Pakistan in order to conserve the remaining remnants. A preliminary reconnaissance survey on the basis of land use divides the narrow stretch of land, with concentration of remnants into four zones. The remnants were further evaluated for in situ conservation by a scoring system based on vegetation analysis and quality of habitat. Low value was assigned to those criteria which had negative impact and high to those which had positive impact on performance of B. monosperma and its long-term survival. Additional information, pertaining to decline, trends of change in the land use, and the underlying socioeconomic processes, was also made. Information from the study area indicated that, in the past two decades, the decline and conversion of the remaining B. monosperma forest to agriculture and forestry has been intensified. The vegetation analysis revealed that all remnants are broadly B. monosperma-Adhatoda vasica, bispecies association. The scoring system ranked the remnants into four classes. Two forests from the intact remnant class and one from the mildly degraded class were recommended for in situ conservation. Keeping in view the deteriorating status of the highly ranked remnants across the landscape and the degree of alteration it is undergoing, they were assigned the conservation status category of "critical." An approach suggested for their immediate conservation was to fence them, with open access to the local people for sustainable resource use. In situ conservation of these benchmarks would help in further understanding the ecological, evolutionary, and structural features of this unique biogeographic ecotone and its affinities with the adjacent ecosystems. PMID:19894131

Khan, Amin U

2010-11-01

340

Direct effects of Moringa oleifera Lam (Moringaceae) acetone leaf extract on broiler chickens naturally infected with Eimeria species.  

PubMed

Avian coccidiosis is one of the most important diseases of poultry and it is responsible for a large number of all broiler mortalities worldwide. The control of this disease relies mainly on the use of anticoccidial drugs. However, herbal preparations could be an alternative for the treatment against coccidiosis in chickens. The direct effects of Moringa oleifera acetone extracts on broiler chickens naturally infected with mixed Eimeria species was investigated to determine the relative efficacy of the extracts against coccidiosis in birds. The investigations were carried out in seven groups (ten chickens per group). The birds were given various doses (1.0, 2.0, 3.0, 4.0 and 5.0 g/kg body weight) of acetone extract of leaves of M. toltrazuril (positive control) and untreated (negative control). The extract was evaluated for anticoccidial activity by means of inhibition of oocyst output in faeces, faecal score, weight gain and mortality. Haematological indices were evaluated by standard methods. The group treated with 1.0 g/ kg body weight Moringa oleifera extract produced the least inhibitory effect on oocyst shed in the faeces (96.4%), while the groups treated with 2.0 g/kg, 3.0 g/kg, 4.0 g/kg and 5.0 g/kg body weight of the extract produced 97.4, 98.7, 99.1 and 99.8%, respectively. Body weight gains of infected chickens treated with the extract significantly improved (p < 0.05), and faecal scores were milder. Packed cell volume, haemoglobin concentration and red blood count of the treated birds were significantly (p < 0.05) higher than those of the infected untreated group. Moringa oleifera leaves could find application in the treatment of avian coccidiosis in veterinary practice. PMID:23440591

Ola-Fadunsin, Shola David; Ademola, Isaiah Oluwafemi

2013-08-01

341

Nutrient and Antinutrient Contents in Raw and Cooked Young Leaves and Immature Pods Of Moringa oleifera, Lam  

Microsoft Academic Search

The nutrient composition and anti-nutrient contents of raw and cooked Moringa leaves and immature pods were analyzed. Cooking caused significant reductions (p ? 0.05) in the contents of crude protein, crude fiber, ether extracts, ash, ascorbic acid, and ?-carotene in the leaves and pods. Significant reductions (p ? 0.05) also were observed in Ca, Mg, Zn, P, Na, Cu, and

A. B. Gidamis; J. T. Panga; S. V. Sarwatt; B. E. Chove; N. B. Shayo

2003-01-01

342

Nutrient Dynamics and Litter Decomposition in Leucaena leucocephala ( Lam.) De Wit Plantation in the Nigerian Derived Savanna  

Microsoft Academic Search

Nutrient contents and rate of litter decomposition were investigated in Leucaena leucocephala plantation in the University of Agriculture, Abeokuta, Ogun State, Nigeria. Litter bag technique was used to study the pattern and rat e of litter decomposition and nutrient release of Leucaena leucocephala . Fifty grams of oven-dried leaf litter of the spec ies was weighed into 0.2 mm mesh

A. O. Oladoye; B. A. Ola-Adams; M. O. Adedire; D. A. Agboola

343

Allelopathic research of subtropical vegetation in Taiwan : III. Allelopathic exclusion of understory byLeucaena leucocephala (Lam.) de Wit.  

PubMed

Leucaena leucocephala plantations in Kaoshu, southern Taiwan, exhibit, after several years of growth, a unique pattern of weed exclusion beneathLeucaena canopy. The pattern has been observed in manyLeucaena plantations in Taiwan and is particularly pronounced in the area where a substantial amount ofLeucaena litter has accumulated on the ground. Field data showed that the phenomenon was primarily not due to physical competition involving light, soil moisture, pH, and nutrients. Instead, aqueous extracts ofLeucaena fresh leaves, litter, soil, and seed exudate showed significantly phytotoxic effects on many test species, including rice, lettuce,Acacia confusa, Alnus formosana, Casuarina glauca, Liquidambar formosana, andMimosa pudica. However, the extracts were not toxic to the growth ofLeucaena seedlings. The decomposing leaves ofLeucaena also suppressed the growth of the aforementioned plants grown in pots but did not inhibit that ofLeucaena plants. By means of paper and thin-layer chromatography, UV-visible spectrophotometry, and high-performance liquid chromatography, 10 phytotoxins were identified. They included mimosine, quercetin, and gallic, protocatechuic,p-hydroxybenzoic,p-hydroxyphenylacetic, vanillic, ferulic, caffeic, andp-coumaric acids. The mature leaves ofLeucaena possess about 5% dry weight of mimosine, the amount varying with varieties. The seed germination and radicle growth of lettuce, rice, and rye grass were significantly inhibited by aqueous mimosine solution at a concentration of 20 ppm, while that of the forest species mentioned was suppressed by the mimosine solution at 50 ppm or above. However, the growth ofMiscanthus floridulus andPinus taiwanensis was not suppressed by the mimosine solution at 200 ppm. The seedlings ofAgeratum conzoides died in mimosine solution at 50 ppm within seven days and wilted at 300 ppm within three days. It was concluded that the exclusion of understory plants was evidently due to the allelopathic effect of compounds produced byLeucaena. The allelopathic pattern was clearly shown in the area with a heavy accumulation ofLeucaena leaf litter, which was a result of drought and heavy wind influence. PMID:24307122

Chou, C H; Kuo, Y L

1986-06-01

344

Self-compatibility in Lolium temulentum L: its genetic control and transfer into L. perenne L. and L. multiflorum Lam  

Microsoft Academic Search

The segregation of self-compatibility from L. temulentum was examined in backcross generations derived from hybrids between this species and the self-incompatible species, L. perenne and L. multiflorum, the latter being used as recurrent parents. Segregation patterns for self-compatibility were determined by percentage self seed set and by in-vitro self-pollination scores in the resulting backcross generations. Self-compatibility in L. temulentum is

D Thorogood; M D Hayward

1992-01-01

345

Genetic Dissection of New Genotypes of Drumstick Tree (Moringa oleifera Lam.) Using Random Amplified Polymorphic DNA Marker  

PubMed Central

The knowledge of genetic diversity of tree crop is very important for breeding and improvement program for the purpose of improving the yield and quality of its produce. Genetic diversity study and analysis of genetic relationship among 20 Moringa oleifera were carried out with the aid of twelve primers from, random amplified polymorphic DNA marker. The seeds of twenty M. oleifera genotypes from various origins were collected and germinated and raised in nursery before transplanting to the field at University Agricultural Park (TPU). Genetic diversity parameter, such as Shannon's information index and expected heterozygosity, revealed the presence of high genetic divergence with value of 1.80 and 0.13 for Malaysian population and 0.30 and 0.19 for the international population, respectively. Mean of Nei's gene diversity index for the two populations was estimated to be 0.20. In addition, a dendrogram constructed, using UPGMA cluster analysis based on Nei's genetic distance, grouped the twenty M. oleifera into five distinct clusters. The study revealed a great extent of variation which is essential for successful breeding and improvement program. From this study, M. oleifera genotypes of wide genetic origin, such as T-01, T-06, M-01, and M-02, are recommended to be used as parent in future breeding program. PMID:23862149

Rufai, Shamsuddeen; Hanafi, M. M.; Rafii, M. Y.; Ahmad, S.; Arolu, I. W.; Ferdous, Jannatul

2013-01-01

346

Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker.  

PubMed

The knowledge of genetic diversity of tree crop is very important for breeding and improvement program for the purpose of improving the yield and quality of its produce. Genetic diversity study and analysis of genetic relationship among 20 Moringa oleifera were carried out with the aid of twelve primers from, random amplified polymorphic DNA marker. The seeds of twenty M. oleifera genotypes from various origins were collected and germinated and raised in nursery before transplanting to the field at University Agricultural Park (TPU). Genetic diversity parameter, such as Shannon's information index and expected heterozygosity, revealed the presence of high genetic divergence with value of 1.80 and 0.13 for Malaysian population and 0.30 and 0.19 for the international population, respectively. Mean of Nei's gene diversity index for the two populations was estimated to be 0.20. In addition, a dendrogram constructed, using UPGMA cluster analysis based on Nei's genetic distance, grouped the twenty M. oleifera into five distinct clusters. The study revealed a great extent of variation which is essential for successful breeding and improvement program. From this study, M. oleifera genotypes of wide genetic origin, such as T-01, T-06, M-01, and M-02, are recommended to be used as parent in future breeding program. PMID:23862149

Rufai, Shamsuddeen; Hanafi, M M; Rafii, M Y; Ahmad, S; Arolu, I W; Ferdous, Jannatul

2013-01-01

347

The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves  

Microsoft Academic Search

Moringa oleifera is used in Thai traditional medicine as cardiotonic. Recent studies demonstrated its hypocholesterolaemic effect. However, to be clinically useful, more scientific data are needed. Aim of the Study: We investigated the antioxidant, hypolipidaemic and antiatherosclerotic activities of Moringa oleifera leaf extract. Materials and Methods: Scavenging activity of the extract on 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), and the inhibitory effect on

Pilaipark Chumark; Panya Khunawat; Yupin Sanvarinda; Srichan Phornchirasilp; Noppawan Phumala Morales; Laddawal Phivthong-ngam; Piyanee Ratanachamnong; Supath Srisawat; Klai-upsorn S. Pongrapeeporn

2008-01-01

348

Physiological and biochemical changes associated with flower development and senescence in so far unexplored Helleborus orientalis Lam. cv. Olympicus.  

PubMed

The so far unexplored H. Orientalis cv. Olympicus exhibits a unique pattern of flower senescence, involving re-greening of creamy white petaloid sepals at the later stages. The greenish sepals become photosynthetically competent immediately after pollination and persist until the seeds are set. After the seed set, the entire (green) flower abscises from the plant. Flower development of Helleborus orientalis cv. Olympicus growing in the open was divided into six stages (I-VI) from tight bud stage to the senescent stage. The average life span of an individual flower after it is fully open is about 6 days. Membrane permeability of sepal tissues estimated as electrical conductivity of leachates increased during senescence. The content of sugars and soluble proteins in the sepal tissues increased during flower opening and declined thereafter during senescence. The protease activity increased as the flower progressed towards senescence. From the present study, it becomes evident that decline in the sugar status and elevation in specific protease activity leading to degradation of proteins are the important factors regulating development and senescence in H. orientalis flowers. Although the tissue content of soluble proteins registered an overall quantitative decrease but SDS-PAGE of protein extract from sepal tissues suggested a decrease in the expression of high molecular weight proteins and an increase in low molecular weight proteins during flower development and senescence. At this stage it is not known whether the polypeptides that increased during senescence play an important role in the senescence of Helleborus orientalis flowers. The increase in these polypeptides during flower senescence is of particular interest because they may be linked to flower longevity. Understanding the nature of these proteins can provide new insights into the pathways that execute senescence and the post-transcriptional regulation of senescence in this flower system. PMID:23572993

Shahri, Waseem; Tahir, Inayatullah; Islam, Sheikh Tajamul; Bhat, Mushtaq Ahmad

2011-03-01

349

LC/MS/NMR analysis of isomeric divanilloylquinic acids from the root bark of Fagara zanthoxyloides Lam.  

PubMed

Gradient HPLC coupled to DAD/UV, MS/MS and NMR has been applied to the rapid structure determination of three new isomeric divanilloylquinic acids from Fagara zanthoxyloides collected in Burkina Faso: 3,4-O-divanilloylquinic acid, 3,5-O-divanilloylquinic acid and 4,5-O-divanilloylquinic acid. Furthermore these new compounds named burkinabins A-C could play a useful role in sickle cell disease, as the active agents of Fagara zanthoxyloïdes are said to be unidentified aromatic compounds with carboxylic acid grouping (Adesanya, S.A., Sofowora, A., 1983. Biological standardisation of Zanthoxylum roots for antisickling activity. Planta Med. 48, 27-33). PMID:15110696

Ouattara, Badioré; Angenot, Luc; Guissou, Pierre; Fondu, Pierre; Dubois, Jacques; Frédérich, Michel; Jansen, Olivia; van Heugen, Jean-Claude; Wauters, Jean-Noël; Tits, Monique

2004-04-01

350

Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers  

Microsoft Academic Search

We used simple sequence repeat (SSR) markers to investigate the genetic diversity of 78 sweet potato (Ipomoea batatas) accessions (58 landraces and 20 putative clones) from traditional agricultural households from 19 local communities in the Vale do Ribeira, São Paulo, Brazil. Eight SSR loci were assessed using 6% (w\\/v) polyacrylamide gels stained with silver nitrate and the accessions genotyped considering

Elizabeth Ann Veasey; Aline Borges; Mariana Silva Rosa; Jurema R. Queiroz-Silva; Eduardo de Andrade Bressan; Nivaldo Peroni

2008-01-01

351

The Hf isotope composition of cratonic mantle: LAM-MC-ICPMS analysis of zircon megacrysts in kimberlites  

Microsoft Academic Search

Zircon megacrysts represent a late stage in the crystallisation of the magmas that produced the low-Cr megacryst suite (Ol+Opx+Cpx+Gnt+Ilm+Phl+Zir) found in many kimberlites, and may carry information on the sources of the parent magmas and the interaction of these magmas with the cratonic lithosphere. The isotopic composition of Hf has been measured in 124 mantle-derived zircon megacrysts from African, Siberian

W. L. Griffin; N. J. Pearson; E. Belousova; S. E. Jackson; E. van Achterbergh; Suzanne Y. O’Reilly; S. R. Shee

2000-01-01

352

A synthetic approach to N-aryl carbamates via copper-catalyzed Chan-Lam coupling at room temperature.  

PubMed

A mild and efficient synthesis of N-arylcarbamates was achieved by reacting azidoformates with boronic acids in the presence of 10 mol % of copper chloride catalyst. The reaction proceeds readily in an open flask at room temperature without additional base, ligand, or additive. Rapid access to urea analogues via a two-step one-pot procedure is enabled by reacting N-arylcarbamates with aluminum-amine complexes. In addition, among several boronic acid derivatives prepared, dimethylphenyl boronate was found to react rapidly in its reaction with benzyl azidoformate, invoking in situ generation of this species in the catalytic cycle. PMID:25584697

Moon, Soo-Yeon; Kim, U Bin; Sung, Dan-Bi; Kim, Won-Suk

2015-02-01

353

Mining Multi-Dimensional Constrained Gradients in Data Cubes Guozhu Dong Jiawei Han Joyce Lam Jian Pei Ke Wang  

E-print Network

, such as trend analysis, answ.C., Canada¡ han, lamd, peijian, wangk¢ @cs.sfu.ca Abstract Constrained gradient analysis (similar, capable of capturing trends in data and answering "what-if" questions. To facilitate our discussion, we

Wang, Ke

354

Performance of Dacryodes edulis (Don. G. Lam H. J.) Seeds and Seedlings in a Crude Oil Contaminated Soil  

Microsoft Academic Search

Environmental pollution from oil activities in a major oil producing country like Nigeria is inevitable. Studies on the effects of crude oil contamination on forest tree species is vital because of the great deal of crude oil spillage in the coastal regions of Nigeria. This paper evaluates the performance of Dacryodes edulis seeds and seedlings in a crude oil contaminated

O. Mary Agbogidi; O. Felix Eshegbeyi

2006-01-01

355

Exchange-Correlation Energy Density from Virial Theorem Federico G. Cruz, Kin-Chung Lam, and Kieron Burke*  

E-print Network

be approximated as a functional of the spin densities. The local spin density (LSD) approximation Exc LSD has long of exact exchange with GGA exchange-correlation. Typical bond energy errors are about 30 kcal/mol in LSD, 8, some have spurious extrema, and overall, look worse than their LSD counterparts. So how do they produce

Burke, Kieron

356

Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass ( Lolium multiflorum Lam.)  

Microsoft Academic Search

Bacterial wilt caused by Xanthomonas translucens pv. graminis (Xtg) is a major disease of economically important forage crops such as ryegrasses and fescues. Targeted breeding based on seedling inoculation has resulted in cultivars with considerable levels of resistance. However, the mechanisms of inheritance of resistance are poorly understood and further breeding progress is difficult to obtain. This study aimed to

Bruno Studer; Beat Boller; Doris Herrmann; Eva Bauer; Ulrich K. Posselt; Franco Widmer; Roland Kölliker

2006-01-01

357

Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam.  

PubMed

Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines. PMID:25189525

Rathi Sre, P R; Reka, M; Poovazhagi, R; Arul Kumar, M; Murugesan, K

2015-01-25

358

Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam  

NASA Astrophysics Data System (ADS)

Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines.

Rathi Sre, P. R.; Reka, M.; Poovazhagi, R.; Arul Kumar, M.; Murugesan, K.

2015-01-01

359

[Functional states of the antioxidant enzymatic complex of tissues of Mytilus galloprovincialis Lam. under conditions of oxidative stress].  

PubMed

There are generalized materials on reaction of enzymatic antioxidant (AO) complex of tissues of molluscs of the genus Mytilus to oxidative loading of different intensity. It is shown that with increase of level of oxidative stress, a rise of activity of glutathione peroxidase (GP) and glutathione reductase (GR) is observed, as well as the rate of glutathione (GSH) in tissues also increases. Then the toxic loading is compensated by a rise of activities of enzymes of low affinity to hydroperoxides, specifically of catalase. In the glutathione system, it is possible to identify several relatively steady states: of low, moderate, and highly intensive processes. In several tissues, functioning of the AO system seems to be directed not to rendering harmless, but to generation of reactive oxygen species (O2-, superoxide dismutase), which is likely to be determined by specificity of their running processes (destruction of damaged byssus theads). The molluscan AO complex is characterized by high lability and sensitivity both to physiological states (spawning) and to action of factors of anthropogenic nature (cationic detergents). Reactions of AO complex of the mussel to the natural states and to toxic action are comparable between each other, which is necessary to be taken into account at diagnostics of the aquatic medium. PMID:25775851

2014-01-01

360

Contact Angle Hysteresis, Adhesion, and Marine Biofouling Donald L. Schmidt, Robert F. Brady, Jr.,*, Karen Lam, Dale C. Schmidt, and  

E-print Network

, adhesion, and marine biofouling were studied. Adhesion was determined by peel tests using pressure-sensitive paints would be an environmentally benign coating from which marine organisms could be easily removed

Chaudhury, Manoj K.

361

On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity  

PubMed Central

New Guinea is considered the most important secondary centre of diversity for sweet potato (Ipomoea batatas). We analysed nuclear and chloroplast genetic diversity of 417 New Guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical America. The molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical America. Nuclear data confirm previous results, suggesting that New Guinea landraces are principally derived from the Northern neotropical genepool (Camote and Batata lines, from the Caribbean and Central America). However, chloroplast data suggest that South American clones (early Kumara line clones or, more probably, later reintroductions) were also introduced into New Guinea and then recombined with existing genotypes. The frequency distribution of pairwise distances between New Guinea landraces suggests that sexual reproduction, rather than somaclonal variation, has played a predominant role in the diversification of sweet potato. The frequent incorporation of plants issued from true seed by farmers, and the geographical and cultural barriers constraining crop diffusion in this topographically and linguistically heterogeneous island, has led to the accumulation of an impressive number of variants. As the diversification of sweet potato in New Guinea is primarily the result of farmers' management of the reproductive biology of their crop, we argue that on-farm conservation programmes that implement distribution of core samples (clones representing the useful diversity of the species) and promote on-farm selection of locally adapted variants may allow local communities to fashion relatively autonomous strategies for coping with ongoing global change. PMID:23531982

Roullier, C; Kambouo, R; Paofa, J; McKey, D; Lebot, V

2013-01-01

362

Effect of Growth Regulators on In Vitro Morphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. Using Mature Zygotic Embryos Explants  

PubMed Central

The percent study describes the in vitro responses of mature zygotic embryos of Boscia senegalensis to different concentrations (0.0–5.0?mg/L) of 6-benzyladnine (BA), Thidiazuron (TDZ), ?-Naphthalene acetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) supplemented on Murashige and Skoog medium (MS). The plant growth regulators (PGRs) were considerably affected the morphogenetic responses. BA produced adventitious shoots through two ways: direct organogenesis and auxiliary shoot formation. Both 2, 4-D and TDZ tend to produce callus, whereas NAA improve the development of embryos to seedlings. Maximum number of shoots/explant (14.8 ± 0.6) was obtained on MS medium supplemented with 3.0?mg/L BA. 67.0% of excised shoots were rooted either on 1/2 MS medium augmented with or without 0.25?mg/L IBA. The highest number of roots (1.2 ± 0.4) and root length (0.5 ± 0.2?cm) was produced on 0.25?mg/L IBA-containing medium. Regenerated plants were successfully acclimatized and transferred to the green house with 70% survival rate. All the plants appeared morphologically uniform with normal growth pattern. A rapid (30 days), efficient and without subculturing protocol for in vitro regeneration of B. senegalensis was developed. PMID:21687567

Daffalla, Hussien H.; Abdellatef, Eltayb; Elhadi, Elsadig A.; Khalafalla, Mutasim M.

2011-01-01

363

Chemical composition and biological activity of the essential oil from leaves of Moringa oleifera Lam. cultivated in Mozambique.  

PubMed

The antioxidant capacity and antimicrobial activity of the essential oil of Moringa oleifera (Moringaceae) grown in Mozambique was investigated. The chemical composition was studied by means of GC and GC-MS analysis. Hexacosane (13.9%), pentacosane (13.3%) and heptacosane (11.4%) were the main components. Ultra High Performance Chromatography-DAD analysis detected the flavonoids quercetin (126 ?g/g) and luteolin (6.2 ?g/g). The essential oil exhibited a relatively low free radical scavenging capacity. The antimicrobial activity of the essential oil was assayed against two Gram-positive strains (Bacillus cereus, Staphylococcus aureus), two Gram-negative strains (Escherichia coli, Pseudomonas aeruginosa), and five fungal strains of agro-food interest (Penicillium aurantiogriseum, Penicillium expansum, Penicillium citrinum, Penicillium digitatum, and Aspergillus niger spp.). B. cereus and P. aeruginosa, as well as the fungal strains were sensitive to the essential oil. PMID:24022760

Marrufo, Tatiana; Nazzaro, Filomena; Mancini, Emilia; Fratianni, Florinda; Coppola, Raffaele; De Martino, Laura; Agostinho, Adelaide Bela; De Feo, Vincenzo

2013-01-01

364

Angle Coverage Maximization in Wireless Sensor Networks Kit-Yee Chow, King-Shan Lui and Edmund Y. Lam  

E-print Network

how well an area or a target is monitored by the sensors [2] [3] [4]. Most of the current studies1 Angle Coverage Maximization in Wireless Sensor Networks Kit-Yee Chow, King-Shan Lui and Edmund Y In this letter, we study the angle coverage problem in wireless sensor networks. We consider the situation

Tam, Vincent W. L.

365

Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing  

Microsoft Academic Search

High-throughput integration site profiling has become a feasible tool to assess vector biosafety and to monitor the cell fate of the gene-corrected cell population in clinical gene therapy studies. Here we report a step-by-step protocol for universal genome-wide and comprehensive integrome analysis that can be performed on >102–103 samples of interest in parallel. This assay is composed of fast and

Anna Paruzynski; Anne Arens; Richard Gabriel; Cynthia C Bartholomae; Simone Scholz; Wei Wang; Stephan Wolf; Hanno Glimm; Christof von Kalle; Manfred Schmidt

2010-01-01

366

From introduced American weed to Cape Verde Islands endemic: the case of Solanum rigidum Lam. (Solanaceae, Solanum subgenus Leptostemonum).  

PubMed

A Solanum species long considered an American introduction to the Cape Verde Islands off the west coast of Africa is identified as Solanum rigidum, a member of the Eggplant clade of Old World spiny solanums (Solanum subgenus Leptostemonum) and is probably endemic to the Cape Verde Islands. Collections of this species from the Caribbean are likely to have been introduced from the Cape Verde Islands on slave ships. We discuss the complex nomenclatural history of this plant and provide a detailed description, illustration and distribution map. The preliminary conservation status of Solanum rigidum is Least Concern, but needs to be reassessed in light of its endemic rather than introduced status. PMID:24198710

Knapp, Sandra; Vorontsova, Maria S

2013-01-01

367

Control-flow analysis of order k (k-CFA)  

E-print Network

BEnv = Var Addr Store = Addr Lam Ã? BEnv = #12;Factored environment Env = Var Lam Ã? Env BEnv = Var Addr Store = Addr Lam Ã? BEnv = #12;Factored environment Env = Var Lam Ã? Env BEnv = Var Addr Store = Addr Clo clo Clo = Lam Ã? BEnv a Addr is a set of addresses #12;Environment look

Might, Matt

368

Berberine.  

PubMed

Berberine is a plant alkaloid with a long history of medicinal use in both Ayurvedic and Chinese medicine. It is present in Hydrastis canadensis (goldenseal), Coptis chinensis (Coptis or goldenthread), Berberis aquifolium (Oregon grape), Berberis vulgaris (barberry), and Berberis aristata (tree turmeric). The berberine alkaloid can be found in the roots, rhizomes, and stem bark of the plants. Berberine extracts and decoctions have demonstrated significant antimicrobial activity against a variety of organisms including bacteria, viruses, fungi, protozoans, helminths, and chlamydia. Currently, the predominant clinical uses of berberine include bacterial diarrhea, intestinal parasite infections, and ocular trachoma infections. PMID:10767672

2000-04-01

369

Spatial pattern and uncertainty of soil carbon and nitrogen in a subtropical savanna landscape in southern Texas  

E-print Network

: Zanthoxylum fagara, Celtis pallida, Condalia hookeri, Diospyros texana, Schaefferia cunefolia, Ziziphus obtusifolia and Berberis trifoliolata. The grassland matrix between woody patches is dominated by C 4 grasses such as Paspalum setaceum, Setaria...

Liu, Feng

2009-05-15

370

Economic Analysis of Selected Bush Management Practices for Eastern South Texas.  

E-print Network

ebony Texas persimmon Twisted acacia White brush Wolfberry Berberis trifoliolata M oric. Acacia rigidula Benth. Cenchrus ciliaris (L.) Link Acacia greggii Gray Leucophyllum frutescens Berl. Bumelia lanuginosa Michx. Schaefferia cuneifolia Gray...

McBryde, G.L.; Conner, J.R.; Scifres C.J.

1984-01-01

371

[Pollen dispersion and reproductive success of four tree species of a xerophytic forest from Argentina].  

PubMed

The "talares" in eastern Buenos Aires province, Argentina, are coastal xerophitic forests structured by few arboreal species surrounded by a lower and moister soil matrix. We studied the reproductive parameters of the most representative arboreal species (Celtis tala, Scutia buxifolia, Jodina rhombifolia, and Schinus longifolia). Pollen dispersion was studied through floral visitor traps (biotic dispersion) and using gravimetric pollen collectors (abiotic dispersion). The reproductive success (fruit formation rate) of the focal species was studied by enclosing flowers with different mesh bags. The reproductive system varied among the different species. C. tala was anemophilous and selfcompatible. S. buxifolia was entomophilous and floral visitors dependant. J. rhombifolia was entomophylous, although spontaneous autogamy could favor reproduction in the absence of pollinators. Lastly, S. longifolia could be an ambophilous species (pollinated by insects and by the wind). This dual system may be the result of system flexibility mechanism or an evolutionary transition. PMID:19637707

Torretta, Juan Pablo; Basilio, Alicia M

2009-01-01

372

Do Ecological Niche Model Predictions Reflect the Adaptive Landscape of Species?: A Test Using Myristica malabarica Lam., an Endemic Tree in the Western Ghats, India  

E-print Network

Ecological niche models (ENM) have become a popular tool to define and predict the “ecological niche” of a species. An implicit assumption of the ENMs is that the predicted ecological niche of a species actually reflects the adaptive landscape...

Nagaraju, Shivaprakash K.; Gudasalamani, Ravikanth; Barve, Narayani; Ghazoul, Jaboury; Narayangowda, Ganeshaiah Kotiganahalli; Ramanan, Uma Shaanker

2013-11-29

373

Arbuscular mycorrhizal fungi associated with Artemisia umbelliformis Lam, an endangered aromatic species in Southern French Alps, influence plant P and essential oil contents.  

PubMed

Root colonization by arbuscular mycorrhizal (AM) fungi of Artemisia umbelliformis, investigated in natural and cultivated sites in the Southern Alps of France, showed typical structures (arbuscules, vesicles, hyphae) as well as spores and mycelia in its rhizosphere. Several native AM fungi belonging to different Glomeromycota genera were identified as colonizers of A. umbelliformis roots, including Glomus tenue, Glomus intraradices, G. claroideum/etunicatum and a new Acaulospora species. The use of the highly mycorrhizal species Trifolium pratense as a companion plant impacted positively on mycorrhizal colonization of A. umbelliformis under greenhouse conditions. The symbiotic performance of an alpine microbial community including native AM fungi used as inoculum on A. umbelliformis was evaluated in greenhouse conditions by comparison with mycorrhizal responses of two other alpine Artemisia species, Artemisia glacialis and Artemisia genipi Weber. Contrary to A. genipi Weber, both A. umbelliformis and A. glacialis showed a significant increase of P concentration in shoots. Volatile components were analyzed by GC-MS in shoots of A. umbelliformis 6 months after inoculation. The alpine microbial inoculum increased significantly the percentage of E-?-ocimene and reduced those of E-2-decenal and (E,E)-2-4-decadienal indicating an influence of alpine microbial inoculum on essential oil production. This work provides practical indications for the use of native AM fungi for A. umbelliformis field culture. PMID:21243378

Binet, Marie-Noëlle; van Tuinen, Diederik; Deprêtre, Nicolas; Koszela, Nathalie; Chambon, Catherine; Gianinazzi, Silvio

2011-08-01

374

Transportation and Traffic Theory 2009 Edited by W.H.K. Lam, S.C. Wong and H.K. Lo  

E-print Network

because of the phenomenon of induced demand. To alleviate traffic congestion in cities, road pricing has to lay out a clear model of traffic congestion that can be applied to cordon pricing, and allows us. The vast literature of congestion pricing can be composed in two categories, marginal-cost pricing models

Levinson, David M.

375

Nine polymorphic microsatellite markers in Centaurea stoebe L. [subspecies C. s. stoebe and C. s. micranthos (S. G. Gmelin ex Gugler) Hayek] and C. diffusa Lam. (Asteraceae)  

Microsoft Academic Search

Centaurea stoebe (subspecies C. s. stoebe and C. s. micranthos (S. G. Gmelin ex Gugler) Hayek) and Centaurea diffusa are Eurasian plant species that have invaded much of North America. We isolated seven microsatellite loci from C. stoebe and two loci from C. diffusa. All loci described here amplify in both species and have between six and 25 alleles each.

ROBIN A. MARRS; RUTH A. HUFBAUER; STEVEN M. BOGDANOWICZ; RENE SFORZA

2006-01-01

376

Effects of interaction between pollen coat eluates and pistil at the molecular level in self-compatible and self-incompatible plants of Lolium multiflorum Lam  

Microsoft Academic Search

Two-dimensional electrophoresis (2-DE) of soluble proteins and enzymes was performed and specific activities of 5 enzymes\\u000a (esterase, pectinesterase, acid phosphatase, protease and diaphorase) were determined in stigmas ofLolium multiflorum (Italian ryegrass) treated with self or foreign pollen coat eluates (pc). Also, a low-molecular-weight fraction of the treated\\u000a self-compatible (SC) and self-incompatible (SI) stigmas was analyzed by high-pressure liquid chromatography (HPLC).

Andrzej Kalinowski; Marek Rad?owski; Aleksandra Bocian

2006-01-01

377

Evaluation of nutritional quality of moringa ( Moringa oleifera Lam.) leaves as an alternative protein source for Nile tilapia ( Oreochromis niloticus L.)  

Microsoft Academic Search

This study was conducted to evaluate the suitability of freeze-dried moringa leaf meal, Moringa oleifera, as an alternative protein source for Nile tilapia. Three experimental diets were formulated to contain moringa leaf meal at levels of 10%, 20% and 30% of the total dietary protein (Diets 2, 3 and 4, respectively) and one diet acting as a control (Diet 1)

Nahid Richter; Perumal Siddhuraju; Klaus Becker

2003-01-01

378

Moringa oleifera Lam. leaf extract prevents early liver injury and restores antioxidant status in mice fed with high-fat diet.  

PubMed

Consumption of high-fat diet (HFD) induces nonalcoholic fatty liver disease (NAFLD) and may lead to multiple complications affecting human health. In the present study, effect of Moringa oleifera leaf extract (MoLE) in alleviating HFD induced liver injury in mice has been reported. Liver histology and serum activity of hepatic marker enzymes i.e. aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) have been studied. Lipid peroxidation (LPO), ferric reducing antioxidant power (FRAP) and reduced glutathione (GSH) were also estimated using liver homogenate. Results of the study suggested that MoLE treatment protected HFD-induced liver damage as indicated by histopathology and liver enzyme activity compared to only-HFD fed group (P < 0.05). Interestingly, early signs of HFD-induced fatty liver were also alleviated by MoLE. Moreover, significant increase in endogenous antioxidant parameters and lower lipid peroxidation were found in liver of all MoLE treated groups. Results of the study indicated that MoLE has both preventive as also curative hepatoprotective activity. PMID:22734251

Das, Nilanjan; Sikder, Kunal; Ghosh, Santinath; Fromenty, Bernard; Dey, Sanjit

2012-06-01

379

Antimicrobial, resistance-modifying effects, antioxidant and free radical scavenging activities of Mezoneuron benthamianum Baill., Securinega virosa Roxb. &Wlld. and Microglossa pyrifolia Lam.  

PubMed

Mezoneuron benthamianum, Securinega virosa and Microglossa pyrifolia are used in folk medicine in Ghana for the treatment of dermal infections and wounds. Petroleum spirit, chloroform and ethanol extracts of the plants were tested for antimicrobial activity against a battery of organisms using the agar well diffusion technique and a serial dilution microassay. The resistance modifying activities of these extracts on standard antibiotics against Staphylococcus aureus possessing efflux mechanisms of resistance have also been assessed. A 4-fold potentiation of the activity of norfloxacin was observed for ethanol and chloroform extracts of M. benthamianum and S. virosa, respectively, whilst the petroleum spirit extract resulted in a 2-fold potentiation with minimum inhibitory concentration (MIC) values in the range 8-16 microg/mL. Ethanol extracts of all three species, the petroleum spirit extract of M. benthamianum and the chloroform extracts of M. benthamianum and S. virosa, showed interesting antimicrobial activities. Antioxidant and free radical scavenging activities using DPPH spectrophotometric and TBA lipid peroxidation assays were also conducted. Of the five extracts that showed antioxidant activities, the petroleum spirit and chloroform extracts of M. benthamianum rated most highly by displaying strong free radical scavenging activity with IC50 values of 15.33 and 19.72 microg/mL, respectively. Lipid peroxidation inhibition provided by the same two extracts also produced the lowest IC50 values for all the extracts tested, of 23.15 and 30.36 microg/mL. These findings therefore give some support to the ethnopharmacological use of the plants in the treatment of various skin diseases and wounds, as well as demonstrating the potential of some of the plants as sources of compounds possessing the ability to modulate bacterial multidrug resistance. PMID:16397919

Dickson, R A; Houghton, P J; Hylands, P J; Gibbons, S

2006-01-01

380

Overexpression of horsegram (Macrotyloma uniflorum Lam.Verdc.) NAC transcriptional factor (MuNAC4) in groundnut confers enhanced drought tolerance.  

PubMed

The NAC family being the largest plant-specific transcription factors functions in diverse and vital physiological processes during development. NAC proteins are known to be crucial in imparting tolerance to plants against abiotic stresses, such as drought and salinity, but the functions of most of them are still elusive. In this study, we report for the first time expression of the MuNAC4, a member of NAC transcription factor from horsegram (Macrotyloma uniflorum) conferring drought tolerance. The groundnut (Arachis hypogaea) transgenics were generated using recombinant MuNAC4 binary vector transformation approach. Molecular analysis of these transgenic lines confirmed the stable gene integration and expression of the MuNAC4 gene. Twelve lines of T5 generation exhibited significantly enhanced tolerance to drought stress with proliferated lateral root growth as compared to wild types. Transgenics exposed to long-term desiccation stress assays showed increased lateral roots and greenish growth. The physiological parameters analysis also suggests that overexpression of MuNAC4 plays a significant role in improving the water stress tolerance of transgenic groundnut, reducing the damage to membrane structures and enhancing osmotic adjustment and antioxidative enzyme regulation under stress. This study validates MuNAC4 as an important candidate gene for future phytoengineering approaches for drought tolerance in crop plants. PMID:24748414

Pandurangaiah, Merum; Lokanadha Rao, G; Sudhakarbabu, O; Nareshkumar, A; Kiranmai, K; Lokesh, U; Thapa, Ganesh; Sudhakar, Chinta

2014-08-01

381

Productivity of alley farming with leucaena ( Leucaena leucocephala Lam. de Wit) and Napier grass ( Pennisetum purpureum K. Schum) in coastal lowland Kenya  

Microsoft Academic Search

Inadequate supply of fodder is a serious constraint to the potentially-promising small-holder-dairy production system in coastal Kenya. Alley farming could be an approach to addressing this problem. A study of forage production based on Napier grass and leucaena in an alley cropping system was conducted on an infertile sandy soil in lowland coastal Kenya. The effects of leucaena hedgerows,Clitoria ternatea

J. G. Mureithi; R. S. Tayler; W. Thorpe

1995-01-01

382

Evaluation of anti-urolithiatic effect of aqueous extract of Bryophyllum pinnatum (Lam.) leaves using ethylene glycol-induced renal calculi  

PubMed Central

Materials and Methods : Thirty-six Wistar male rats were randomly divided into six equal groups. Group A animals received distilled water for 28 days. Group B to group F animals received 1% v/v ethylene glycol in distilled water for 28 days and group B served as ethylene glycol control. Groups C and D (preventive groups) received aqueous extract of leaves of B. pinnatum 50 and 100 mg/kg intraperitoneally, respectively for 28 days. Groups E and F (treatment groups) received aqueous extract of leaves of B. pinnatum 50 and 100 mg/kg intraperitoneally, respectively from 15th to 28th day. On days 0 and 28, 24 hrs urine samples were collected for urinary volume and urinary oxalate measurement. On day 28, blood was collected for serum creatinine and blood urea level monitoring. All animals were sacrificed and kidneys were removed, weighed, and histopathologically evaluated for calcium oxalate crystals deposition. Results: Administration of aqueous extract of leaves of B. pinnatum reduced urine oxalate level ?significantly, as compared with Group B (p<0.001). Serum creatinine and blood urea level were ?improved significantly in all aqueous extract of leaves of B. pinnatum-treated groups. Relative ?kidney weight and calcium oxalate depositions were found significantly reduced in animals ?received ABP as compared with Group B (p<0.001). ? Conclusions: B. pinnatum is effective in prevention and treatment of ethylene glycol-induced urolithiasis. PMID:25050313

Shukla, Apexa Bhanuprasad; Mandavia, Divyesh Rasikbhai; Barvaliya, Manish Jasmatbhai; Baxi, Seema Natvarlal; Tripathi, Chandrabhanu Rajkishore

2014-01-01

383

Herbicidal activity of Brassicaceae seed meal on wild oat (Avena fatua), Italian ryegrass (Lolium multiflorum Lam.), redroot pigweed (Amaranthus retroflexus L.) and prickly lettuce (Lactuca serriola)  

Technology Transfer Automated Retrieval System (TEKTRAN)

There is an on-going need for the development of sustainable methods of weed control in crop production systems. Studies were conducted to evaluate the efficacy of different Brassicaceae seed meals and application rates on the emergence of several weed species including wild oat, Italian rye grass, ...

384

African eggplant (Solanum anguivi Lam.) fruit with bioactive polyphenolic compounds exerts in vitro antioxidant properties and inhibits Ca2+-induced mitochondrial swelling  

PubMed Central

Objective To evaluate the antioxidant and radical scavenging activities of Solanum anguivi fruit (SAG) and its possible effect on mitochondrial permeability transition pore as well as mitochondrial membrane potential (??m) isolated from rat liver. Methods Antioxidant activity of SAG was assayed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power, iron chelation and ability to inhibit lipid peroxidation in both liver and brain homogenate of rats. Also, the effect of SAG on mitochondrial membrane potential and mitochondrial swelling were determined. Identification and quantification of bioactive polyphenolics was done by HPLC-DAD. Results SAG exhibited potent and concentration dependent free radical-scavenging activity (IC50/DPPH=275.03±7.8 µg/mL). Reductive and iron chelation abilities also increase with increase in SAG concentration. SAG also inhibited peroxidation of cerebral and hepatic lipids subjected to iron oxidative assault. SAG protected against Ca2+ (110 µmol/L)-induced mitochondrial swelling and maintained the ??m. HPLC analysis revealed the presence of gallic acid [(17.54±0.04) mg/g], chlorogenic acid (21.90±0.02 mg/g), caffeic acid (16.64±0.01 mg/g), rutin [(14.71±0.03) mg/g] and quercetin [(7.39±0.05) mg/g]. Conclusions These effects could be attributed to the bioactive polyphenolic compounds present in the extract. Our results suggest that SAG extract is a potential source of natural antioxidants that may be used not only in pharmaceutical and food industry but also in the treatment of diseases associated with oxidative stress. PMID:24075339

Elekofehinti, Olusola Olalekan; Kamdem, Jean Paul; Bolingon, Aline Augusti; Athayde, Margareth Linde; Lopes, Seeger Rodrigo; Waczuk, Emily Pansera; Kade, Ige Joseph; Adanlawo, Isaac Gbadura; Rocha, Joao Batista Teixeira

2013-01-01

385

Protective effects of friedelin isolated from Azima tetracantha Lam. against ethanol-induced gastric ulcer in rats and possible underlying mechanisms.  

PubMed

The current study was aimed to investigate the gastroprotective effects of friedelin isolated from the hexane extract of leaves of Azima tetracantha. Ethanol-induced gastric ulcer model was used to investigate the gastroprotective effects of friedelin. Antioxidant enzymes, lipid peroxidation, nitric oxide, gastric vascular permeability, pro and anti-inflammatory cytokines and apoptosis level have been investigated. Ethanol caused severe gastric damage and friedelin pretreatment protected against its deleterious role. Antioxidant enzyme activities, anti-inflammatory cytokines, prostaglandin E2 (PGE2), constitutive nitric oxide synthase (cNOS) and mucus weight have been increased significantly. However, the vascular permeability, pro-inflammatory cytokines, inducible nitric oxide synthase (iNOS), caspase-3 and apoptosis level have significantly been decreased after friedelin ingestion. The present study has clearly demonstrated the anti-ulcer potential of friedelin, these findings suggested that friedelin could be a new useful natural gastroprotective tool against gastric ulcer. PMID:25617794

Antonisamy, Paulrayer; Duraipandiyan, Veeramuthu; Aravinthan, Adithan; Al-Dhabi, Naif Abdullah; Ignacimuthu, Savarimuthu; Choi, Ki Choon; Kim, Jong-Hoon

2015-03-01

386

Facile Synthesis of a Tungsten Alkylidyne Catalyst for Alkyne Zachary J. Tonzetich, Yan Choi Lam, Peter Muller, and Richard R. Schrock*  

E-print Network

Facile Synthesis of a Tungsten Alkylidyne Catalyst for Alkyne Metathesis Zachary J. Tonzetich, Yan cis double bonds. Tungsten alkylidyne trialkoxide alkyne metathesis catalysts were discovered in 1981 of cleavage of a tungsten-tungsten triple bond upon reaction with an alkyne or nitrile.9 Recent advances

Müller, Peter

387

Isolation and characterisation of cDNA clones representing the genes encoding the major tuber storage protein (dioscorin) of yam (Dioscorea cayenensis Lam.)  

Microsoft Academic Search

cDNA clones encoding dioscorins, the major tuber storage proteins (Mr 32000) of yam (Dioscorea cayenensis) have been isolated. Two classes of clone (A and B, based on hybrid release translation product sizes and nucleotide sequence differences) which are 84.1% similar in their protein coding regions, were identified. The protein encoded by the open reading frame of the class A cDNA

R. Steven Conlan; Leslie-Ann Griffiths; Johnathan A Napier; Peter R Shewry; Sinclair Mantell; Charles Ainsworth

1995-01-01

388

Isolation and characterisation of cDNA clones representing the genes encoding the major tuber storage protein (dioscorin) of yam (Dioscorea cayenensis Lam.).  

PubMed

cDNA clones encoding dioscorins, the major tuber storage proteins (M(r) 32,000) of yam (Dioscorea cayenesis) have been isolated. Two classes of clone (A and B, based on hybrid release translation product sizes and nucleotide sequence differences) which are 84.1% similar in their protein coding regions, were identified. The protein encoded by the open reading frame of the class A cDNA insert is of M(r) 30,015. The difference in observed and calculated molecular mass might be attributed to glycosylation. Nucleotide sequencing and in vitro transcription/translation suggest that the class A dioscorin proteins are synthesised with signal peptides of 18 amino acid residues which are cleaved from the mature peptide. The class A and class B proteins are 69.6% similar with respect to each other, but show no sequence identity with other plant proteins or with the major tuber storage proteins of potato (patatin) or sweet potato (sporamin). Storage protein gene expression was restricted to developing tubers and was not induced by growth conditions known to induce expression of tuber storage protein genes in other plant species. The codon usage of the dioscorin genes suggests that the Dioscoreaceae are more closely related to dicotyledonous than to monocotyledonous plants. PMID:7632909

Conlan, R S; Griffiths, L A; Napier, J A; Shewry, P R; Mantell, S; Ainsworth, C

1995-06-01

389

A rapid in vitro multiplication system for commercial propagation of pharmaceutically important Cyclea peltata (Lam) Hook & Thoms. based on enhanced axillary branching  

Microsoft Academic Search

An efficient and quick in vitro propagation of Cyclea peltata by repeated subculture of nodal cuttings has been standardized. The nodal cuttings were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations (0.5–7mg\\/l) of 6-benzylaminopurine (BA) or kinetin (Kn) alone or in combination with indole-3-acetic acid (IAA; 0.5mg\\/l) for culture initiation. Optimum response in terms of percent cultures

Jyothi Abraham; Meena K. Cheruvathur; Bince Mani; T. Dennis Thomas

2010-01-01

390

A Secure Image Transfer Application for the NHSnet Kerry Jean kjean@ee.ucl.ac.uk, Walter Eaves weaves@ee.ucl.ac.uk, John Lam  

E-print Network

parties to enable mobile health care practitioners to securely transfer images into the NHS network while results of the testing of the application. An evaluation of the effects of the different security sensitive, so communication and co-operation require appropriate security services to be instigated

Haddadi, Hamed

391

Effect of home preparation practices on the content of provitamin A carotenoids in coloured sweet potato varieties ( Ipomoea batatas Lam.) from Kenya  

Microsoft Academic Search

The content of ?-carotene was determined in raw peeled, boiled peeled and roasted peeled roots of six orange- and yellow-fleshed sweet potato varieties from Kenya to evaluate their vitamin A potential before and after household preparation. The boiling and roasting procedures were similar to traditional methods used in Kenya. Dried products, chips and flour, of the variety Zapallo were also

Ulla Kidmose; Lars P. Christensen; Sammy M. Agili; Shakuntala H. Thilsted

2007-01-01

392

Arbuscular mycorrhizal fungi associated with Artemisia umbelliformis Lam, an endangered aromatic species in Southern French Alps, influence plant P and essential oil contents  

Microsoft Academic Search

Root colonization by arbuscular mycorrhizal (AM) fungi of Artemisia umbelliformis, investigated in natural and cultivated sites in the Southern Alps of France, showed typical structures (arbuscules, vesicles,\\u000a hyphae) as well as spores and mycelia in its rhizosphere. Several native AM fungi belonging to different Glomeromycota genera\\u000a were identified as colonizers of A. umbelliformis roots, including Glomus tenue, Glomus intraradices, G.

Marie-Noëlle Binet; Diederik van Tuinen; Nicolas Deprêtre; Nathalie Koszela; Catherine Chambon; Silvio Gianinazzi

2011-01-01

393

Cloudworks as a 'pedagogical wrapper' for LAMS sequences: supporting the sharing of ideas across professional boundaries and facilitating collaborative design, evaluation and critical reflection  

Microsoft Academic Search

Cloudworks is a specialised social networking site for sharing, debating and co- creating ideas as well as designs and resources for teaching, learning and scholarship in education. The site has been co-funded by JISC and The Open University, and has ca.2500 registered users and visitors from 165 countries (May 2010). Fundamental to the development of the site has been the

Rebecca Galley; Gráinne Conole; James Dalziel; Ernie Ghiglione

394

Using the Internet in Middle Schools: A Model for Success. A Collaborative Effort between Los Alamos National Laboratory (LANL) and Los Alamos Middle School (LAMS).  

ERIC Educational Resources Information Center

Los Alamos National Laboratory (LANL) developed a model for school networking using Los Alamos Middle School as a testbed. The project was a collaborative effort between the school and the laboratory. The school secured administrative funding for hardware and software; and LANL provided the network architecture, installation, consulting, and…

Addessio, Barbara K.; And Others

395

Introduction of Annual Forage Species (Secale cereale L. and Lolium multiflorum Lam.) into Permanent Swards: A Technique to Improve Early Season Herbage Production and Nitrogen Uptake  

Microsoft Academic Search

Permanent grassland soils can release surplus nitrogen (N) in autumn and winter but herbage growth is reduced by soil N deficiency in spring. The hypothesis that winter-growing rye or Italian ryegrass, oversown (strip-seeded) in autumn into permanent swards, might reduce these limitations was investigated in field trials in western Britain. Introduction of Italian ryegrass did not increase spring production and

Alan Hopkins; Timothy M. Martyn; Peter J. Bowling

1997-01-01

396

Parsing dimensional versus diagnostic category-related patterns of reward circuitry function in behaviorally and emotionally dysregulated youth in the Longitudinal Assessment of Manic Symptoms (LAMS) study  

PubMed Central

Context Pediatric disorders characterized by behavioral and emotional dysregulation pose diagnostic and treatment challenges because of high comorbidity, suggesting that they may be better conceptualized dimensionally rather than categorically. Identifying neuroimaging measures associated with behavioral and emotional dysregulation in youth may inform understanding of underlying dimensional vs. disorder-specific pathophysiology. Objective Identify, in a large cohort of behaviorally and emotionally dysregulated youth, neuroimaging measures that: 1) are associated with behavioral and emotional dysregulation pathological dimensions (behavioral and emotional dysregulation measured with the Parent General Behavior Inventory 10 Item Mania Scale [PGBI-10M], mania, depression, anxiety); or 2) differentiate diagnostic categories(BPSD, ADHD, anxiety, disruptive behavior disorders (DBD)). Design Multi-site neuroimaging study(February 2011–April 2012). Setting Academic medical centers: Case Western Reserve University, Cincinnati Children’s Hospital, University of Pittsburgh. Patients Referred sample of behaviorally and emotionally dysregulated youth(n=85) from the Longitudinal Assessment of Manic Symptoms study and healthy youth (n=20). Main Outcome Measures Region-of-interest analyses examined relationships among prefrontal-ventral striatal reward circuitry during a reward paradigm (Win, Loss, control conditions), symptom dimensions, and diagnostic categories. Results Regardless of diagnosis, higher PGBI-10M scores were associated with greater left middle prefrontal cortical (mPFC; r=0.28), and greater levels of anxiety with greater right dorsal anterior cingulate cortical (dACC; r=0.27), activity to Win. The 20 highest (t=2.75) and 20 lowest (t=2.42) PGBI-10M scoring youth showed significantly greater left mPFC activity to Win than 20 healthy youth. DBD were associated with lower left ventrolateral prefrontal cortex(VLPFC) activity to Win (t=2.68) (all ps<0.05, corrected). Conclusions Greater PGBI-10M-related left mPFC activity, and greater anxiety-related right dACC activity, to Win may reflect heightened reward sensitivity and greater attention to reward in behaviorally and emotionally dysregulated youth, regardless of diagnosis. Reduced left VLPFC activity to Win may reflect reward insensitivity in youth with DBD. Despite a distinct reward-related neurophysiology in DBD, findings generally support a dimensional approach to studying neural mechanisms in behaviorally and emotionally dysregulated youth. PMID:24285346

Bebko, Genna; Bertocci, Michele A.; Fournier, Jay C.; Hinze, Amanda K.; Bonar, Lisa; Almeida, Jorge R. C.; Perlman, Susan B.; Versace, Amelia; Schirda, Claudiu; Travis, Michael; Gill, Mary Kay; Demeter, Christine; Diwadkar, Vaibhav A.; Ciuffetelli, Gary; Rodriguez, Eric; Olino, Thomas; Forbes, Erika; Sunshine, Jeffrey L.; Holland, Scott K; Kowatch, Robert. A.; Birmaher, Boris; Axelson, David; Horwitz, Sarah M.; Arnold, L. Eugene; Fristad, Mary. A; Youngstrom, Eric A.; Findling, Robert L.; Phillips, Mary L.

2014-01-01

397

The antioxidant activity and free radical-scavenging capacity of dietary phenolic extracts from horse gram ( Macrotyloma uniflorum (Lam.) Verdc.) seeds  

Microsoft Academic Search

Total phenolics and the antioxidative properties of two varieties of horse gram (Macrotyloma uniflorum) were studied. The raw and dry-heated seed samples were extracted successively with methanol and 70% acetone separately. After removing the solvents, the extracts were freeze-dried. The black seeds contained relatively high levels of total phenolics and tannins than the brown seeds with respect to the treatments

Perumal Siddhuraju; Sellamuthu Manian

2007-01-01

398

A Nonhost Peptidase Inhibitor of ~14?kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera  

PubMed Central

Helicoverpa armigera is one of the major devastating pests of crop plants. In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H. armigera larvae. B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14?kDa with or without reduction. In vitro studies towards total gut proteolytic enzymes of H. armigera and bovine trypsin indicated measurable inhibitory activity. B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively. The IC50 of B. monosperma peptidase inhibitor against total H. armigera gut proteinases activity was 2.0?µg/mL. The larval feeding assays suggested B. monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H. armigera. Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced. The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H. armigera suggest its usefulness in insect pest management of food crops. PMID:24860667

Pandey, Prabhash K.; Singh, Dushyant; Singh, Sangram; Khan, M. Y.; Jamal, Farrukh

2014-01-01

399

The movement of air in and out of the lungs is accom-plished by the contraction of voluntary muscles (Lam-  

E-print Network

postnatal ability of the respiratory muscula- ture to maintain vigorous, continuous, coordinated func- tion of continuous breathing at birth, including hyperoxia (Baier et al., 1992), umbilical cord occlusion (Adamson contractions, extrusion through the birth canal, and abrupt exposure to a cold postpartum environment (Ronca et

400

Chemical composition and general toxicity of essential oils extracted from the aerial parts of Artemisia armeniaca Lam. and A. incana (L.) Druce growing in Iran.  

PubMed

The essential oils of the aerial parts of A. armeniaca and A. incana, collected from Arasbaran area (East Azarbaijan province, Iran) were extracted by hydrodistillation and analyzed by GC-MS. In total, 16 and 40 constituents were identified and quantified in the oils of A. armeniaca and A. incana representing 80.5% and 84.6% of the oils, respectively. The essential oil of A. armeniaca was mainly composed of non-terpene hydrocarbons (24.8%). The major components of the oil were ?-pinene (10.7%), nonadecane (10.0%), 6,10,14-trimethyl-2-pentadecanone (9.4%), spathulenol (7.8%) and Z-verbenol (5.8%). The essential oil of A. incana was dominated by oxygenated monoterpenes (41.6%), with camphor (20.4%), 1,8-cineol (10.3%), Z-verbenol (8.7%), ?-thujone (8.3%) and ?-thujone (5.6%), as major components. The essential oils were also subjected to general toxicity assay using brine shrimp lethality method. The toxicity profile of both oils indicated some degree of toxicity in comparison with podophyllotoxin. PMID:24459478

Mojarrab, M; Delazar, A; Esnaashari, S; Afshar, F Heshmati

2013-01-01

401

The antidiabetic effect of low doses of Moringa oleifera Lam. seeds on streptozotocin induced diabetes and diabetic nephropathy in male rats.  

PubMed

The antidiabetic activity of two low doses of Moringa seed powder (50 and 100?mg/kg body weight, in the diet) on streptozotocin (STZ) induced diabetes male rats was investigated. Forty rats were divided into four groups. The diabetic positive control (STZ treated) group showed increased lipid peroxide, increased IL-6, and decreased antioxidant enzyme in the serum and kidney tissue homogenate compared with that of the negative control group. Immunoglobulins (IgA, IgG), fasting blood sugar, and glycosylated hemoglobin (HbA1c) were also increased as a result of diabetes in G2 rats. Moreover albumin was decreased, and liver enzymes and ?-amylase were not affected. In addition, the renal functions and potassium and sodium levels in G2 were increased as a sign of diabetic nephropathy. Urine analysis showed also glucosuria and increased potassium, sodium, creatinine, uric acid, and albumin levels. Kidney and pancreas tissues showed also pathological alteration compared to the negative control group. Treating the diabetic rats with 50 or 100?mg Moringa seeds powder/kg body weight in G3 and G4, respectively, ameliorated the levels of all these parameters approaching the negative control values and restored the normal histology of both kidney and pancreas compared with that of the diabetic positive control group. PMID:25629046

Al-Malki, Abdulrahman L; El Rabey, Haddad A

2015-01-01

402

Anti-EGFR Antibody Efficiently and Specifically Inhibits Human TSC2?\\/? Smooth Muscle Cell Proliferation. Possible Treatment Options for TSC and LAM  

Microsoft Academic Search

Background: Tuberous sclerosis complex (TSC), a tumor syndrome caused by mutations in TSC1 or TSC2 genes, is characterized by the development of hamartomas. We previously isolated, from an angiomyolipoma of a TSC2 patient, a homogenous population of smooth muscle-like cells (TSC22\\/2 ASM cells) that have a mutation in the TSC2 gene as well as TSC2 loss of heterozygosity (LOH) and

Elena Lesma; Vera Grande; Silvia Ancona; Stephana Carelli; Anna Maria Di Giulio; Alfredo Gorio

2008-01-01

403

Anti-EGFR Antibody Efficiently and Specifically Inhibits Human TSC2-\\/- Smooth Muscle Cell Proliferation. Possible Treatment Options for TSC and LAM  

Microsoft Academic Search

Background Tuberous sclerosis complex (TSC), a tumor syndrome caused by mutations in TSC1 or TSC2 genes, is characterized by the development of hamartomas. We previously isolated, from an angiomyolipoma of a TSC2 patient, a homogenous population of smooth muscle-like cells (TSC2?\\/? ASM cells) that have a mutation in the TSC2 gene as well as TSC2 loss of heterozygosity (LOH) and

Elena Lesma; Vera Grande; Silvia Ancona; Stephana Carelli; Anna Maria di Giulio; Alfredo Gorio; Rory Edward Morty

2008-01-01

404

DCEG Award Recipients - Anand Pathak, MD, PhD, MPH, Olusegun Onabajo, PhD, Clara Lam, PhD, MPH  

Cancer.gov

To understand the role of IFN-?4 during HCV infection, we performed detailed functional analysis of IFN-?4. We suggest that a pathogenic effect of IFN-?4 expression in human hepatic cells is associated with inhibition of cell proliferation causing increased cell death and induction of pro-inflammatory cytokines. Chronic induction of IFN-?4 in HCV-infected hepatocytes leading to cell death and tissue repair may result in hepatic fibrosis and cirrhosis, which are relevant factors affecting clearance of HCV and, possibly progression to hepatocellular carcinoma.

405

Larvicidal, ovicidal, and adulticidal efficacy of Erythrina indica (Lam.) (Family: Fabaceae) against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae).  

PubMed

Mosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis, and Japanese encephalitis. Mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. In view of the recently increased interest in developing plant origin insecticides as an alternative to chemical insecticide, this study was undertaken to assess the larvicidal, ovicidal, and adulticidal potential of the crude hexane, benzene, chloroform, ethyl acetate, and methanol solvent extracts from the medicinal plant Erythrina indica against the medically important mosquito vectors, Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae). The larval mortality was observed after 24 h of exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in methanol extract of leaf of E. indica against the larvae of A. stephensi, A. aegypti, and C. quinquefasciatus with the LC50 and LC90 values of 69.43, 75.13, and 91.41 ppm and 125.49, 134.31, and 167.14 ppm, respectively. The mean percent hatchability of the eggs was observed after 48 h post treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. All the five solvent extracts showed moderate ovicidal activity; however, the methanol extract showed the highest ovicidal activity. The methanol extract of E. indica against A. stephensi, A. aegypti, and C. quinquefasciatus exerted 100 % mortality (zero hatchability) at 150, 200, and 250 ppm, respectively. Control eggs showed above 99.3-100 % hatchability. The adult mortality was observed after 24 h recovery period. The plant crude extracts showed dose-dependent mortality. At higher concentrations, the adult showed restless movement for some times with abnormal wagging and then died. Among the extracts tested, the highest adulticidal activity was observed in methanol extract against A. stephensi followed by A. aegypti and C. quinquefasciatus with the LD50 and LD90 values of 88.76, 94.09, and 119.64 ppm and 160.83, 169.01, and 219.77 ppm, respectively. No mortality was recorded in the control. Our data suggest that the crude hexane, benzene, chloroform, ethyl acetate, and methanol solvent extracts of E. indica have the potential to be used as an eco-friendly approach for the control of the A. stephensi, A. aegypti, and C. quinquefasciatus. PMID:24322290

Govindarajan, Marimuthu; Sivakumar, Rajamohan

2014-02-01

406

Comparison of Content and In vitro Bioaccessibility of Provitamin A Carotenoids in Home Cooked and Commercially Processed Orange Fleshed Sweet Potato (Ipomea batatas Lam).  

PubMed

Vitamin A deficiency (VAD) remains a public health problem in some regions of Brazil. Increased use of orange-fleshed sweet potato (OFSP) as a source of pro-vitamin A represents a potential strategy for prevention of VAD. We compared the pro-vitamin A content, vitamin A equivalency and bioaccessibility of ?-carotene (?C) of two varieties of home cooked OFSP and two commercial sources of processed OFSP. Pro-vitamin A carotenoid content in home cooked, Beauregard variety of OFSP exceeded that in Amelia variety and commercial products for babies. All-trans-?C was the most abundant carotenoid in raw, cooked and commercial OFSP. Boiling and frying OFSP generally decreased total ?C. A serving of 100 g FW Beauregard variety of cooked OFSP contained greater than 100 % of the estimated average requirement (EAR) for children and women, and up to 92 % EAR for lactating women. Although the efficiency of micellarization of all-trans-?C during simulated digestion of OFSP was relatively low (4-8 %) and significantly less than for cis-isomers, the quantities of trans-?C incorporated into micelles from boiled Beauregard and fried Amelia varieties exceeded that in micelles generated by digesting commercial OFSP. The bioaccessibility of pro-vitamin A carotenoids in the micelle fraction of digested OFSP was confirmed with differentiated cultures of Caco-2 human intestinal cells. Continued development of OFSP such as the Amelia and Beauregard varieties that are rich in trans-?C and dissemination of best practices for home cooking are encouraged to increase consumption of this food to decrease the risk of vitamin A deficiency in Brazil. PMID:25575485

Berni, Paulo; Chitchumroonchokchai, Chureeporn; Canniatti-Brazaca, Solange G; De Moura, Fabiana F; Failla, Mark L

2015-03-01

407

The Antidiabetic Effect of Low Doses of Moringa oleifera Lam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats  

PubMed Central

The antidiabetic activity of two low doses of Moringa seed powder (50 and 100?mg/kg body weight, in the diet) on streptozotocin (STZ) induced diabetes male rats was investigated. Forty rats were divided into four groups. The diabetic positive control (STZ treated) group showed increased lipid peroxide, increased IL-6, and decreased antioxidant enzyme in the serum and kidney tissue homogenate compared with that of the negative control group. Immunoglobulins (IgA, IgG), fasting blood sugar, and glycosylated hemoglobin (HbA1c) were also increased as a result of diabetes in G2 rats. Moreover albumin was decreased, and liver enzymes and ?-amylase were not affected. In addition, the renal functions and potassium and sodium levels in G2 were increased as a sign of diabetic nephropathy. Urine analysis showed also glucosuria and increased potassium, sodium, creatinine, uric acid, and albumin levels. Kidney and pancreas tissues showed also pathological alteration compared to the negative control group. Treating the diabetic rats with 50 or 100?mg Moringa seeds powder/kg body weight in G3 and G4, respectively, ameliorated the levels of all these parameters approaching the negative control values and restored the normal histology of both kidney and pancreas compared with that of the diabetic positive control group. PMID:25629046

Al-Malki, Abdulrahman L.; El Rabey, Haddad A.

2015-01-01

408

Sveconorwegian crustal underplating in southwestern Fennoscandia: LAM-ICPMS U Pb and Lu Hf isotope evidence from granites and gneisses in Telemark, southern Norway  

Microsoft Academic Search

Laser ablation ICPMS U Pb and Lu Hf isotope data on granitic-granodioritic gneisses of the Precambrian Vråvatn complex in central Telemark, southern Norway, indicate that the magmatic protoliths crystallized at 1201 ± 9 Ma to 1219 ± 8 Ma, from magmas with juvenile or near-juvenile Hf isotopic composition (176Hf\\/177Hf = 0.2823 ± 11, epsilon-Hf > + 6). These data provide

T. Andersen; W. L. Griffin; A. G. Sylvester

2007-01-01

409

Sveconorwegian crustal underplating in southwestern Fennoscandia: LAM-ICPMS U–Pb and Lu–Hf isotope evidence from granites and gneisses in Telemark, southern Norway  

Microsoft Academic Search

Laser ablation ICPMS U–Pb and Lu–Hf isotope data on granitic-granodioritic gneisses of the Precambrian Vråvatn complex in central Telemark, southern Norway, indicate that the magmatic protoliths crystallized at 1201±9 Ma to 1219±8 Ma, from magmas with juvenile or near-juvenile Hf isotopic composition (176Hf\\/177Hf=0.2823±11, epsilon-Hf>+6). These data provide supporting evidence for the depleted mantle Hf-isotope evolution curve in a time period where juvenile

T. Andersen; W. L. Griffin; A. G. Sylvester

2007-01-01

410

A Nonhost Peptidase Inhibitor of ~14?kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera.  

PubMed

Helicoverpa armigera is one of the major devastating pests of crop plants. In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H. armigera larvae. B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14?kDa with or without reduction. In vitro studies towards total gut proteolytic enzymes of H. armigera and bovine trypsin indicated measurable inhibitory activity. B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively. The IC50 of B. monosperma peptidase inhibitor against total H. armigera gut proteinases activity was 2.0?µg/mL. The larval feeding assays suggested B. monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H. armigera. Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced. The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H. armigera suggest its usefulness in insect pest management of food crops. PMID:24860667

Pandey, Prabhash K; Singh, Dushyant; Singh, Sangram; Khan, M Y; Jamal, Farrukh

2014-01-01

411

Status of the South American tuberous legume Pachyrhizus tuberosus (Lam.) Spreng.: Field observations, taxonomic analysis, linguistic studies and agronomic data on the diversity of the South American Pachyrhizus tuberosus (Lam.) Spreng. complex with special reference to the identification of two new cultivar groups from Ecuador and Peru  

Microsoft Academic Search

The tuberous legume Pachyrhizus tuberosus is found in cultivation sporadically throughout the tropical lowlands of South America.\\u000a As a result of field studies conducted in Bolivia, Ecuador, Peru and Venezuela, three distinct cultivar groups have been identified.\\u000a Rare references presenting further evidence in support of this grouping, and yielding information on the cultivation history,\\u000a are reviewed. The differences in morphology,

MARTEN SØRENSEN; SØREN DØYGAARD; JAIME E. ESTRELLA; LARS PETER KVIST; POUL ERIK NIELSEN

1997-01-01

412

CYCLICITY OF LUSZTIG'S STRATIFICATION OF GRASSMANNIANS AND POISSON GEOMETRY  

E-print Network

a second, Poisson geometric proof of the result of Knutson, Lam, and Speyer that the Coxeter element. In a recent work Knutson, Lam, and Speyer proved that the Lusztig stratification of the Grass- mannian Gr

Caenepeel, Stefaan

413

Characterization of a Truncated Lipoarabinomannan from the Actinomycete Turicella otitidis  

PubMed Central

Lipoarabinomannan (LAM) lipoglycans have been characterized from a range of mycolic acid-containing actinomycetes and from the amycolate actinomycete Amycolatopsis sulphurea. To further understand the structural diversity of this family, we have characterized the lipoglycan of the otic commensal Turicella otitidis. T. otitidis LAM (TotLAM) has been determined to consist of a mannosyl phosphatidylinositol anchor unit carrying an (? 1?6)-linked mannan core and substituted with terminal-arabinosyl branches. Thus, TotLAM has a novel truncated LAM structure. Using the human monocytic THP-1 cell line, it was found that TotLAM exhibited only minimal ability to induce tumor necrosis factor alpha. These findings contribute further to our understanding of actinomycete LAM diversity and allow further speculation as to the correlation between LAM structure and the immunomodulatory activities of these lipoglycans. PMID:15659663

Gilleron, Martine; Garton, Natalie J.; Nigou, Jérôme; Brando, Thérèse; Puzo, Germain; Sutcliffe, Iain C.

2005-01-01

414

25 years of NWP at INM E. Rodriguez CaminoE. Rodriguez Camino  

E-print Network

attempts (19671985).First attempts (19671985). ECMWF LAM + SMHI ANAL (19861995)ECMWF LAM + SMHI ANAL Anal (finite differences, 0.91ºx0.91 19L) + OI Anal (SMHI) (Hortal, DíazPabón, Jimeno) (SMHI) (Hortal

Vali, Gabor

415

Original article Efficacy and foliar absorption of flupyrsulfuron-methyl  

E-print Network

of flupyrsulfuron-methyl and prosulfocarb used to control Lolium multiflorum Lam. in wheat (Triticum aestivum L conçus pour améliorer la lutte contre Lolium multiflorum Lam. dans le blé (Triticum aestivum L.) ont été

Paris-Sud XI, Université de

416

Leaf dynamics, stemflow and throughfall water and nutrient inputs in a subtropical savanna parkland, Texas  

E-print Network

Deciduous Coriaceous Deciduous/ Goriacecus everrIreen Smooth Tree oz' ~; 1, 3 rounded crown Smooth Shrub or tree 1, 3 Smooth Small tree 1, 3 or large shrub 1, 3 Spiny ha~ (~Mlt' ll'da Il Agarito (Berberis trifoliolata Moric. ) Deciduous... rn 20 n In 2 400 BERBERIS DI 0 8PYR0 8 ZANTHOXYLUM LSD sso[ . : LSD~ 80 m 40 rn r Z 80 rn 40 g 60 E E ~ 40 IL IL 20 HAIL STORM o ~ * 's oo 60 E 40 E I- IL 20 HAIL STORM o t J S N J J S N J 1987 1988 1989 DATE J...

Angerer, Jay Peter

1991-01-01

417

Generalized Iteration and Coiteration for Higher-Order Nested Datatypes  

E-print Network

Work supported by: PhD Prg. Logic in Computer Science, Munich (DFG) EU TYPES Project Slide 2 Powerlists · some Haskell datatypes PList a = Zero a | Succ (PList (a,a)) List a = Nil | Cons a (List a) Bush a (List a) PList a = Zero a | Succ (PList (a,a)) Lam a = Var a | App (Lam a) (Lam a) | Abs (Lam (Maybe a

Abel, Andreas

418

Laser Additive Manufacturing and Bionics: Redefining Lightweight Design  

Microsoft Academic Search

New layer wise manufacturing technologies such as Laser Additive Manufacturing (LAM) allow innovative approaches to product design. Especially for lightweight design in aircraft applications LAM offers new possibilities for load-adapted structures. However, to fully capture lightweight potential of LAM technologies new design guidelines and processes have to be developed. A novel approach to extreme lightweight design is realized by incorporating

C. Emmelmann; P. Sander; J. Kranz; E. Wycisk

2011-01-01

419

Ramularia pusilla Ung. and Ramulaspera holci-lanati (Cav.) Lind. in New Zealand  

Microsoft Academic Search

Ramularia pusilla Ung. was recorded on the following grasses in New Zealand:—Agrostis gigantea L., Agrostis stolonifera L., Agrostis tenuis Sibth., Anthoxanthum odoratum L., Bromus catharticus Vahl., Lolium multiflorum Lam., Lolium perenne L., Lolium perenne L. × Lolium multiflorum Lam., Lolium multiflorum Lam. × Festuca gigantea Vill., Lolium perenne L. × Festuca arundinacea Schreb., Festuca rubra L. subsp. commutata Gaud., and

G. C. M. Latch

1964-01-01

420

Principal Investigator/Program Director (Last, First, Middle):___________________________ PHS 398/2590 (Rev. 05/01) Continuation Format Page  

E-print Network

, HG Sperling 1976 The color receptor identities of goldfish cones. Science 191:487-489. Marc RE, HG, DMK Lam 1978 GABAergic pathways in the goldfish retina. J Comp Neu- rol 182:221-246. Lam DMK, YYT Su decarboxylase in the goldfish retina. Nature 278:565-567 Marc RE, DMK Lam 1981 Uptake of aspartic and glutamic

Marc, Robert E.

421

Twisting the quantum grassmannian and T H Lenagan  

E-print Network

of the recent result of Knutson, Lam and Speyer that the Lusztig strata of the classical grassmannian = (12 . . . n) acts on the totally nonnegative grass- mannian. Recently, Knutson, Lam and Speyer, [9 by c; this gives a Poisson geometric proof of Knutson, Lam and Speyer result. The research

Lenagan, Tom

422

Imbalanced Plasminogen System in Lymphangioleiomyomatosis Potential Role of Serum Response Factor  

Microsoft Academic Search

Pulmonary lymphangioleiomyomatosis (LAM) is characterized by abnormal smooth muscle-like cell (LAM cell) proliferation leading to tissue destruction. We previously demonstrated that serum re- sponse factor (SRF), a critical smooth muscle transcription factor, is highly expressed in LAM cells. Here we show that a high SRF level alters the plasminogen (Plg) system. Specifically, overexpression of SRF in human lung fibroblasts upregulated

Xiaoning Zhe; Yan Yang; Lucia Schuger

423

Generalized Iteration and Coiteration for HigherOrder Nested Datatypes  

E-print Network

Work supported by: PhD Prg. Logic in Computer Science, Munich (DFG) EU TYPES Project Slide 2 Powerlists . some Haskell datatypes PList a = Zero a | Succ (PList (a,a)) List a = Nil | Cons a (List a) Bush a = Nil | Cons a (List a) PList a = Zero a | Succ (PList (a,a)) Lam a = Var a | App (Lam a) (Lam a) | Abs

Abel, Andreas

424

Survey of Ilex crenata along the Callaway Gardens Holly Trail: summer 1990  

E-print Network

; Site is heavily shaded by maple 6 pine. Do not replant Fair condition; CN Jap Holly Dwarf Yellow; DR 1974 Ilex crenata 62-W-166 cv. Braddock Heights Fair condition; DR 1962 Ilex crenata cv. Buxifolia Ilex crenata cv. Changsha 56-S-023 54-SP... 56-W-090-001 T Fair condition; Has suckers and sapsucker damage; tag is girdling a limb; DR 1956 Ilex crenata cv. Compacta 63-W-113-002 X NOT FOUND; DR 1963 Ilex crenata cv. Conners 62-W-083 NOT FOUND; DR 1962 Ilex crenata cv. Convexa...

Labega, Adrienne L

1993-01-01

425

A truncated lipoglycan from mycobacteria with altered immunological properties  

PubMed Central

Maintenance of cell-wall integrity in Mycobacterium tuberculosis is essential and is the target of several antitubercular drugs. For example, ethambutol targets arabinogalactan and lipoarabinomannan (LAM) biosynthesis through the inhibition of several arabinofuranosyltransferases. Apart from their role in cell-wall integrity, mycobacterial LAMs also exhibit important immunomodulatory activities. Here we report the isolation and detailed structural characterization of a unique LAM molecule derived from Mycobacterium smegmatis deficient in the arabinofuranosyltransferase AftC (AftC-LAM). This mutant LAM expresses a severely truncated arabinan domain completely devoid of 3,5-Araf–branching residues, revealing an intrinsic involvement of AftC in the biosynthesis of LAM. Furthermore, we found that ethambutol efficiently inhibits biosynthesis of the AftC-LAM arabinan core, unambiguously demonstrating the involvement of the arabinofuranosyltransferase EmbC in early stages of LAM-arabinan biosynthesis. Finally, we demonstrate that AftC-LAM exhibits an enhanced proinflammatory activity, which is due to its ability to activate Toll-like receptor 2 (TLR2). Overall, our efforts further describe the mechanism of action of an important antitubercular drug, ethambutol, and demonstrate a role for specific arabinofuranosyltransferases in LAM biosynthesis. In addition, the availability of sufficient amounts of chemically defined wild-type and isogenic truncated LAMs paves the way for further investigations of the structure–function relationship of TLR2 activation by mycobacterial lipoglycans. PMID:20133807

Birch, Helen L.; Alderwick, Luke J.; Appelmelk, Ben J.; Maaskant, Janneke; Bhatt, Apoorva; Singh, Albel; Nigou, Jerome; Eggeling, Lothar; Geurtsen, Jeroen; Besra, Gurdyal S.

2010-01-01

426

A truncated lipoglycan from mycobacteria with altered immunological properties.  

PubMed

Maintenance of cell-wall integrity in Mycobacterium tuberculosis is essential and is the target of several antitubercular drugs. For example, ethambutol targets arabinogalactan and lipoarabinomannan (LAM) biosynthesis through the inhibition of several arabinofuranosyltransferases. Apart from their role in cell-wall integrity, mycobacterial LAMs also exhibit important immunomodulatory activities. Here we report the isolation and detailed structural characterization of a unique LAM molecule derived from Mycobacterium smegmatis deficient in the arabinofuranosyltransferase AftC (AftC-LAM). This mutant LAM expresses a severely truncated arabinan domain completely devoid of 3,5-Araf-branching residues, revealing an intrinsic involvement of AftC in the biosynthesis of LAM. Furthermore, we found that ethambutol efficiently inhibits biosynthesis of the AftC-LAM arabinan core, unambiguously demonstrating the involvement of the arabinofuranosyltransferase EmbC in early stages of LAM-arabinan biosynthesis. Finally, we demonstrate that AftC-LAM exhibits an enhanced proinflammatory activity, which is due to its ability to activate Toll-like receptor 2 (TLR2). Overall, our efforts further describe the mechanism of action of an important antitubercular drug, ethambutol, and demonstrate a role for specific arabinofuranosyltransferases in LAM biosynthesis. In addition, the availability of sufficient amounts of chemically defined wild-type and isogenic truncated LAMs paves the way for further investigations of the structure-function relationship of TLR2 activation by mycobacterial lipoglycans. PMID:20133807

Birch, Helen L; Alderwick, Luke J; Appelmelk, Ben J; Maaskant, Janneke; Bhatt, Apoorva; Singh, Albel; Nigou, Jerome; Eggeling, Lothar; Geurtsen, Jeroen; Besra, Gurdyal S

2010-02-01

427

Improved detection of alkaloids in crude extracts applying capillary electrophoresis with field amplified sample injection  

Microsoft Academic Search

A simple and effective method for the sensitive detection of alkaloids in crude plant extracts applying capillary electrophoresis with field amplified sample injection (FASI) is described. This method was compared with normal pressure injection for the determination of alkaloids in methanolic extracts from roots of Berberis vulgaris L. (Berberidaceae) and Hydrastis canadensis L. (Ranunculaceae) using a 1:1 mixture of 200

Matthias Unger; Joachim Stöckigt

1997-01-01

428

Successes in conserving the Barberry Carpet moth Pareulype berberata (D. & S.) (Geometridae) in England  

Microsoft Academic Search

Since the end of the 19th century the Barberry Carpet moth (Pareulype berberata) has declined from being widespread and fairly well distributed in England to highly localised and endangered, due mainly to large-scale removal of the sole larval foodplant Berberis vulgaris. In the 1980s the moth appeared to be restricted to a single site. Since 1987 the moth has been

Paul Waring

2004-01-01

429

Prevalence and Distribution of Common Barberry, the Alternate Host of Puccinia graminis , in Minnesota  

Microsoft Academic Search

Peterson, P. D., Leonard, K. J., Miller, J. D., Laudon, R. J., and Sutton, T. B. 2005. Prevalence and distribution of common barberry, the alternate host of Puccinia graminis, in Minnesota. Plant Dis. 89:159-163. A federal and state program operated from 1918 until the 1980s to eradicate common barberry (Berberis vulgaris), the alternate host of Puccinia graminis, from the major

P. D. Peterson; K. J. Leonard; J. D. Miller; R. J. Laudon; T. B. Sutton

2005-01-01

430

Environmentally Friendly Natural Dyeing of Organic Cotton  

Microsoft Academic Search

In this study, organic cotton fabrics were dyed with different natural dye sources (madder root, walnut shell, henna, horse chestnut, pomegranate peel, berberis vulgaris root, thyme, and sage tea). The dyeing was carried out with different mordants (copper sulphate, potassium aluminum sulphate, potassium tartrate, and citric acid), using pre-mordanting dyeing methods. The color of the fabrics was investigated in terms

Mustafa Tutak; N. Ebru Korkmaz

2012-01-01

431

Fertilisation of the Barberry  

Microsoft Academic Search

C. K. SPRENGEL, in his Entdeckte Geheimniss der Natur im Bau und in der Befruchtung der Blumen, gives an excellent account of the structure of the Common Barberry, Berberis vulgaris, and points out how it is visited by insects, and how, upon the touch of an insect's limb or proboscis, the irritable filaments move inwards, and press the opened anthers

T. H. Farrer

1870-01-01

432

EFFICACY OF BARBERRY AQUEOUS EXTRACTS DENTAL GEL ON CONTROL OF PLAQUE AND GINGIVITIS  

Microsoft Academic Search

Herbal extracts have been successfully used in dentistry as tooth cleaning and antimicrobial plaque agents. The aim of this study was to evaluate the clinical effects of a dental gel containing barberry extracts (from Berberis vulgaris) on gingivitis and microbial plaque control. A double blind clinical trial study was conducted in a dormitory on 45 boys aged 11-12 years having

A. Makarem; N. Khalili; R. Asodeh

433

Potential of fungi for the biological control of some New Zealand weeds  

Microsoft Academic Search

The potential for fungi to control 14 important weeds in New Zealand is reviewed. Information provided includes the fungi already known from these weeds in New Zealand, fungi recorded from the native ranges of the weeds, and past work on the control of these weeds using fungi, both in New Zealand and elsewhere. The 14 weeds are: Berberis spp.; Buddleja

Peter R. Johnston

1990-01-01

434

Adaptation of wheat rusts to the wheat cultivars in former Czechoslovakia  

Microsoft Academic Search

In former Czechoslovakia virulence of rusts attacking wheat was studied since the sixties. Since the same time genes for resistance in the registered cultivars were identified. The role of Berberis and Thalictrum as alternate hosts for stem rust and leaf rust, respectively, was investigated as well. Determined changes of virulence in rust populations could only partially be ascribed to changes

Pavel Bartoš; Eva Stuchlíková; Renata Hanušová

1996-01-01

435

Whitebrush Response to Tebuthiuron and Picloram Pellets.  

E-print Network

arborea Quercus stellata Opuntia sp. Setaria macrostachya Smilax bona-nox Aster spinosus Celtis pallida Opuntia leptocaulis Colubrina texensis Diospyros texana Stipa leucotricha Aristida sp. Acacia tortuosa Quercus nigra Ambrosia psilostachya... . Yellow thistle * - - Callicarpa americana Berberis trifoliolata Lycium berlandieri Acacia ridigula Quercus marilandica Buchloe dactyloides Leuchophyllum fru tescens Ambrosia artemisiifolia Hilaria berlangeri Lantana horrida Crataegus mollis...

Scifres, C.J.; Embry, D.L.; Mutz, J.L.

1981-01-01

436

Evaluation of a large-scale invasive plant species herbicide control program in the Berkshire Taconic Plateau, Massachusetts, USA  

Microsoft Academic Search

SUMMARY In the late 1990's, The Nature Conservancy (TNC) documented a rise in five invasive plant species, barberry Berberis thunbergii , bittersweet Celastrus orbiculatus , garlic mustard Alliaria petiolata , buckthorn Frangula alnus , and honeysuckle Lonicera morrowii on the periphery of the relatively intact and uninvaded 14,600 ha Berkshire Taconic Plateau in Massachusetts (USA). The Plateau comprises an ecologically

Lindsay McAlpine; Stephen Porder

437

Antifungal activity of some Himalayan medicinal plants and cultivated ornamental species  

Microsoft Academic Search

Extracts of roots of Rumex nepalensis, Berberis aristata, Arnebia benthamii, bark of Taxus wallichiana, Juglans regia and petals of Jacquinia ruscifolia were tested for their antifungal activity against twelve different fungal pathogens. Ethanolic extracts of R. nepalensis and J. ruscifolia extracts showed a broad spectrum of activity.

Radhey Shyam Sharma; Vandana Mishra; Ram Singh; Nidhi Seth; C. R. Babu

2008-01-01

438

Antifungal activity of some Himalayan medicinal plants and cultivated ornamental species.  

PubMed

Extracts of roots of Rumex nepalensis, Berberis aristata, Arnebia benthamii, bark of Taxus wallichiana, Juglans regia and petals of Jacquinia ruscifolia were tested for their antifungal activity against twelve different fungal pathogens. Ethanolic extracts of R. nepalensis and J. ruscifolia extracts showed a broad spectrum of activity. PMID:18672040

Sharma, Radhey Shyam; Mishra, Vandana; Singh, Ram; Seth, Nidhi; Babu, C R

2008-12-01

439

IR-4 Ornamental Horticulture Program Research Report Form  

E-print Network

, Figures 1 and 2b). At weeks 6, 7 and 8 these differences were significant at 10%. Some plants in all group and plants treated with Freehand (Tables 1 and 6c, Figure 2c). Plant Growth. No significant aquifolium (Berberis aquifolium) Phytotoxicity. There were no significant differences in phytotoxicity

Lieth, J. Heinrich

440

INVASIVE SHRUBS AND SONGBIRD NESTING SUCCESS: EFFECTS OF CLIMATE VARIABILITY AND PREDATOR ABUNDANCE  

Microsoft Academic Search

Previous studies have demonstrated that songbirds often use exotic plants as nesting substrates and may suffer elevated predation rates relative to nests placed in native plants. Veeries (Catharus fuscescens) frequently build nests in an exotic shrub, Japanese barberry (Berberis thunbergii), in forests of southeastern New York State, USA. We monitored Veery nesting success over a six-year period and supplemented these

Kenneth A. Schmidt; Lisa C. Nelis; Nathan Briggs; Richard S. Ostfeld

2005-01-01

441

ReseaRch at the University of Maryland Climate Modeling and Prediction  

E-print Network

for farmers and agricultural policy makers Antonio Busalacchi studies tropical ocean circulation to refine to predict the complex atmospheric effects of polar ice loss. Improving Rainfall Forecasts for Farmers Rapid. Berbery also studies regional mechanisms that force monsoon precipitation over North and South America

Hill, Wendell T.

442

Carbon Dioxide Exchange by Several Streamside and Scrub Oak Community Species of Red Butte Canyon, Utah  

Microsoft Academic Search

ABSTRACT: Species of a xeric scrub oak community (scrub oak, Quercus gambelii; sagebrush, Artemisia tridentata; bitterbrush, Purshia have lower seasonal water potentials than species of a mesic streamside habitat (box elder, Acer negundo; bigtooth maple, Acer grandidentatum; creeping barberry, Berberis repens). The restriction of box elder to mesic sites is, in part, a function of its physiological responses to moisture

Stephen J. Dina; Lionel G. Klikoff

2007-01-01

443

Growth response of native shrubs to acid mine spoil and to proposed soil amendments  

Microsoft Academic Search

Successful reclamation of acid mine sites may be enhanced by revegetating with species that are tolerant to acid mine spoil conditions. This study was conducted to assess the response of four native shrub species, Amelanchier alnifolia Nutt., Symphoricarpos albus (L.) Blake, Berberis repens Lindl., and Ceanothus sanguineus Pursh, to 1) pyritic acid mine spoil amended with various levels of lime

Pamela J. Voeller; Benjamin A. Zamora; James Harsh

1998-01-01

444

Seasonal variation of upper-level mobile trough development upstream of the Pacific storm track  

E-print Network

by Petterssen and Smebye (1971). Berbery and Vera (1996) asserted that evolution as well as typical periods and wavelengths of synoptic-scale eddies responsible for the SH storm track are similar to those observed in NH using the techniques both of measuring...

Myoung, Boksoon

2002-01-01

445

Impact of Honey Bees on the California Environment Eric C. Mussen -Extension Apiculturist, UC Davis -2/4/02  

E-print Network

, if nectar is abundant, will produce a honey crop. While honeys vary in color and taste depending upon floral and honey bees into the environment, natural habitats were altered in many other ways. With honey bees. Asters ­ Aster spp. Azalea ­ Rhododendron spp. Barberry ­ Berberis spp. Beardtongue - Penstemon spp

Ferrara, Katherine W.

446

PREVALENCE AND DISTRIBUTION OF COMMON BARBERRY, THE ALTERNATE HOST OF PUCCINIA GRAMINIS, IN MINNESOTA  

Technology Transfer Automated Retrieval System (TEKTRAN)

A Federal and State program operated from 1918 until the 1980s to eradicate barberry (Berberis vulgaris), the alternate host of Puccinia graminis, from the major areas of cereal production in the U.S. More than 500 million barberry bushes were destroyed nationally during the program; approximately ...

447

Xeriscape...Landscape Water Conservation  

E-print Network

) Barberry Berberis thunbergii atropurpurea 1,3,4,5,6 Sun Red evergreen foliage Bridal wreath spirea Spirea cantoniensis (S. reevesiana) 7 Sun/shade Sprawling; fern-like; white spring blooms China rose Rosa chinensis 1,2,3,4,6 Sun Hardy; long-blooming; pest...

Welsh, Douglas F.; Welch, William C.; Duble, Richard L.

2007-04-27

448

Population diversity of Puccinia graminis is sustained through sexual cycle on alternate hosts  

Technology Transfer Automated Retrieval System (TEKTRAN)

A high degree of virulence diversity has been maintained in the population of Puccinia graminis f. sp. tritici (Pgt) in northwestern United States. Although Berberis vulgaris is present in the region and Pgt has been isolated from aecial infections on B. vulgaris, the population is too diverse to be...

449

First enantioselective total synthesis of (-)-tejedine.  

PubMed

[structure: see text] The first enantioselective total synthesis of (-)-tejedine (1) is reported. Tejedine is a seco-bisbenzyltetrahydroisoquinoline isolated in 1998 as a minor component from Berberis vulgaris. The synthesis was achieved using a strategy employing four key steps, including a chiral auxiliary-assisted diastereoselective Bischler-Napieralski cyclization. PMID:12153207

Wang, You-Chu; Georghiou, Paris E

2002-08-01

450

The effects of small-scale environmental heterogeneity on seed germination in experimental treefall gaps in New Zealand  

Microsoft Academic Search

Seed germination of woody species was studied in treefall gaps in New Zealand to assess how environmental heterogeneity affects regeneration from seed. Gaps were created in a relictual Pinus radiata plantation destined for restoration to native forest. Seeds of the native species Alectryon excelsus, Macropiper excelsum, and Fuch- sia excorticata, and the invasive alien species Cytisus scoparius and Berberis darwinii

Kate G. McAlpine; Donald R. Drake

2002-01-01

451

Prognostic impact of day 15 blast clearance in risk-adapted remission induction chemotherapy for younger patients with acute myeloid leukemia: long-term results of the multicenter prospective LAM-2001 trial by the GOELAMS study group.  

PubMed

Early response to chemotherapy has a major prognostic impact in acute myeloid leukemia patients treated with a double induction strategy. Less is known about patients treated with standard-dose cytarabine and anthracycline. We designed a risk-adapted remission induction regimen in which a second course of intermediate-dose cytarabine was delivered after standard "7+3" only if patients had 5% or more bone marrow blasts 15 days after chemotherapy initiation (d15-blasts). Of 823 included patients, 795 (96.6%) were evaluable. Five hundred and forty-five patients (68.6%) had less than 5% d15-blasts. Predictive factors for high d15-blasts were white blood cell count (P<0.0001) and cytogenetic risk (P<0.0001). Patients with fewer than 5% d15-blasts had a higher complete response rate (91.7% vs. 69.2%; P<0.0001) and a lower induction death rate (1.8% vs. 6.8%; P=0.001). Five-year event-free (48.4% vs. 25%; P<0.0001), relapse-free (52.7% vs. 36.9%; P=0.0016) and overall survival (55.3% vs. 36.5%; P<0.0001) were significantly higher in patients with d15-blasts lower than 5%. Multivariate analyses identified d15-blasts and cytogenetic risk as independent prognostic factors for the three end points. Failure to achieve early blast clearance remains a poor prognostic factor even after early salvage. By contrast, early responding patients have a favorable outcome without any additional induction course. (ClinicalTrials.gov identifier NCT01015196). PMID:23975179

Bertoli, Sarah; Bories, Pierre; Béné, Marie C; Daliphard, Sylvie; Lioure, Bruno; Pigneux, Arnaud; Vey, Norbert; Delaunay, Jacques; Leymarie, Vincent; Luquet, Isabelle; Blanchet, Odile; Cornillet-Lefebvre, Pascale; Hunault, Mathilde; Bouscary, Didier; Fegueux, Nathalie; Guardiola, Philippe; Dreyfus, François; Harousseau, Jean Luc; Cahn, Jean Yves; Ifrah, Norbert; Récher, Christian

2014-01-01

452

Effects of dehydrated methanol extracts of moringa ( Moringa oleifera Lam.) leaves and three of its fractions on growth performance and feed nutrient assimilation in Nile tilapia ( Oreochromis niloticus (L.))  

Microsoft Academic Search

A 10-week feeding trial was conducted in a recirculation system at (27±0.5 °C) to determine the effect of a methanol extract of moringa leaf meal and its different fractions. Nine isonitrogenous and isoenergetic diets containing 35% crude protein and 20 MJ kg?1 gross energy were used. All the experimental diets contained the same amount of fish meal. The inclusion of moringa methanol

Euloge Dongmeza; Perumal Siddhuraju; George Francis; Klaus Becker

2006-01-01

453

Prognostic impact of day 15 blast clearance in risk-adapted remission induction chemotherapy for younger patients with acute myeloid leukemia: long-term results of the multicenter prospective LAM-2001 trial by the GOELAMS study group  

PubMed Central

Early response to chemotherapy has a major prognostic impact in acute myeloid leukemia patients treated with a double induction strategy. Less is known about patients treated with standard-dose cytarabine and anthracycline. We designed a risk-adapted remission induction regimen in which a second course of intermediate-dose cytarabine was delivered after standard “7+3” only if patients had 5% or more bone marrow blasts 15 days after chemotherapy initiation (d15-blasts). Of 823 included patients, 795 (96.6%) were evaluable. Five hundred and forty-five patients (68.6%) had less than 5% d15-blasts. Predictive factors for high d15-blasts were white blood cell count (P<0.0001) and cytogenetic risk (P<0.0001). Patients with fewer than 5% d15-blasts had a higher complete response rate (91.7% vs. 69.2%; P<0.0001) and a lower induction death rate (1.8% vs. 6.8%; P=0.001). Five-year event-free (48.4% vs. 25%; P<0.0001), relapse-free (52.7% vs. 36.9%; P=0.0016) and overall survival (55.3% vs. 36.5%; P<0.0001) were significantly higher in patients with d15-blasts lower than 5%. Multivariate analyses identified d15-blasts and cytogenetic risk as independent prognostic factors for the three end points. Failure to achieve early blast clearance remains a poor prognostic factor even after early salvage. By contrast, early responding patients have a favorable outcome without any additional induction course. (ClinicalTrials.gov identifier NCT01015196) PMID:23975179

Bertoli, Sarah; Bories, Pierre; Béné, Marie C.; Daliphard, Sylvie; Lioure, Bruno; Pigneux, Arnaud; Vey, Norbert; Delaunay, Jacques; Leymarie, Vincent; Luquet, Isabelle; Blanchet, Odile; Cornillet-Lefebvre, Pascale; Hunault, Mathilde; Bouscary, Didier; Fegueux, Nathalie; Guardiola, Philippe; Dreyfus, François; Harousseau, Jean Luc; Cahn, Jean Yves; Ifrah, Norbert; Récher, Christian

2014-01-01

454

Engineering the Primary Substrate Specificity of Streptomyces griseus Trypsin Michael J. Page, Sui-Lam Wong, Jeff Hewitt, Natalie C. J. Strynadka, and Ross T. A. MacGillivray*,  

E-print Network

Engineering the Primary Substrate Specificity of Streptomyces griseus Trypsin Michael J. Page, Sui, 2003; ReVised Manuscript ReceiVed May 22, 2003 ABSTRACT: Streptomyces griseus trypsin (SGT) was chosen Arg or Lys side chains (3, 6, 8). Streptomyces griseus trypsin (SGT)2 was initially purified from

Strynadka, Natalie

455

Seed isotopic analysis as a tool to understand ecological processes influencing plant development and physiology: the case study of Quercus rotundifolia Lam. in a desertification gradient in Mediterranean areas  

NASA Astrophysics Data System (ADS)

Plant responses to climate change highly depend on the temporal variability in precipitation events and on plant specific strategies, such as drought tolerance and resilience. Within the different plant organs, seeds have become an important research tool in the past years to study plant development and nutrients allocation. Key features of seeds such as the tendency to accumulate and store nutrient compounds open many possibilities to explore isotope analysis (13C, 15N and 18O), with many outcomes in fields from ecology to food traceability. The application of light stable isotopes to plant materials have been used to study both physiological (i.e. photosynthesis and stomatal conductance), nutrients uptake and metabolism (origin of nitrogen and symbiotic associations) as well as many ecological processes, which will produce a distinctive isotope fingerprint on the plant tissues. Thus, the isotopic composition of certain bio and geo-elements of seeds, yielding relevant information on plant ecophysiology, are able to relate the plant functioning with local climatic conditions (e.g., temperature and precipitation). The application of isotope analysis in this way can be used as a proxy to understand complex environmental degradation processes such as land degradation in drylands resulting from various factors including climatic variations and human activities. In this study acorns of Quercus ilex plants were sampled during 2012-2013 in a region of southern Portugal, according to (i) soil land-use; (ii) aridity and desertification indexes. The approach developed combined plant seed analysis (seed morphology and compounds quantification) with isotope ratio mass spectrometry (?13C, ?15N and ?18O) as a "tool" to study changes in plant ecophysiology over time and space. Seeds allow studies at specific temporal scale (development period) which varies according to its biology and depends on the climatic conditions where the plant is grown (i.e, seed's biomass integrate climate variations information of several months). The results indicate a clear relationship between seed morphology and both temperature and precipitation as well a significant correlation between ?15N and precipitation, which indicate an influence of major climatic variables on seed carbon allocation and nitrogen uptake. These results may also contribute to future mitigation programmes in degraded areas where there are systematic problems with plant regeneration and ultimately to learn about the application of stable isotopes approaches in dryland ecosystems.

Oliveira, Tatiana; Silva, Anabela; Rodrigues, Carla; Antunes Antunes, Cristina; Pinho, Pedro; Ramos, Alzira; João Pereira, Maria; Branquinho, Cristina; Máguas, Cristina

2014-05-01

456

Forced expression of laminin beta1 in podocytes prevents nephrotic syndrome in mice lacking laminin beta2, a model for Pierson syndrome.  

PubMed

Pierson syndrome is a congenital nephrotic syndrome with ocular and neurological defects caused by mutations in LAMB2, the gene encoding the basement membrane protein laminin ?2 (Lam?2). It is the kidney glomerular basement membrane (GBM) that is defective in Pierson syndrome, as Lam?2 is a component of laminin-521 (LM-521; ?5?2?1), the major laminin in the mature GBM. In both Pierson syndrome and the Lamb2(-/-) mouse model for this disease, laminin ?1 (Lam?1), a structurally similar homolog of Lam?2, is marginally increased in the GBM, but it fails to fully compensate for the loss of Lam?2, leading to the filtration barrier defects and nephrotic syndrome. Here we generated several lines of Lam?1 transgenic mice and used them to show that podocyte-specific Lam?1 expression in Lamb2(-/-) mice abrogates the development of nephrotic syndrome, correlating with a greatly extended lifespan. In addition, the more Lam?1 was expressed, the less urinary albumin was excreted. Transgenic Lam?1 expression increased the level of Lam?5 in the GBM of rescued mice, consistent with the desired increased deposition of laminin-511 (?5?1?1) trimers. Ultrastructural analysis revealed occasional knob-like subepithelial GBM thickening but intact podocyte foot processes in aged rescued mice. These results suggest the possibility that up-regulation of LAMB1 in podocytes, should it become achievable, would likely lessen the severity of nephrotic syndrome in patients carrying LAMB2 mutations. PMID:21876163

Suh, Jung Hee; Jarad, George; VanDeVoorde, Rene G; Miner, Jeffrey H

2011-09-13

457

The laser additive manufacture of Ti6Al4V  

Microsoft Academic Search

Laser additive manufacturing (LAM) is a manufacturing technique with cost-reduction potential for titanium aerospace components. The mechanical properties of LAM Ti-6Al-4V have been investigated extensively, but little work on microstructure evolution has been performed to date. The results presented here provide a first look at the relationships between LAM processing parameters and microstructure in as-deposited Ti-6Al-4V.

P. A. Kobryn; S. L. Semiatin

2001-01-01

458

The laser additive manufacture of Ti6Al4V  

Microsoft Academic Search

Laser additive manufacturing (LAM) is a manufacturing technique with cost-reduction potential for titanium aerospace components.\\u000a The mechanical properties of LAM Ti-6Al-4V have been investigated extensively, but little work on microstructure evolution\\u000a has been performed to date. The results presented here provide a first look at the relationships between LAM processing parameters\\u000a and microstructure in as-deposited Ti-6Al-4V.

P. A. Kobryn; S. L. Semiatin

2001-01-01

459

Field Data Analysis for a Range-Based Local Airport Monitor for WAAS  

Microsoft Academic Search

The Local Airport Monitor (LAM) concept has been proposed as an inexpensive and rapidly deployable implementation of LAAS. The LAM combines WAAS corrections with local monitoring to provide an error bound tight enough to enable Category I precision approach and landing. Two different strategies for LAM have been proposed-a range-based method and a position-domain method (1,2). Both methods monitor WAAS

Jiwon Seo; Jason Rife; Sam Pullen; Todd Walter; Per Enge

460

The Myth and the Mystery of Aja Nye  

E-print Network

to the western heavenly abode... Thirteen centuries after Guru Rinpoche made the above prophecy, a sacred nye (gnas) of Aja in Mongar still holds a mystical attraction for many Bhutanese pilgrims. This sacred nye is attributed to Guru Rinpoche who brought... in political affairs of Trashigang forced Sersanglam to leave for Tibet temporarily, while Aja Lam was assassinated at Jangphu. However, Sersanglam returned to Bhutan, perhaps after the place had become stable. The late Aja Lam's nephew Lam Sharchung stayed...

Wangchuk, Rinzin

2004-01-01

461

The neural crest lineage as a driver of disease heterogeneity in Tuberous Sclerosis Complex and Lymphangioleiomyomatosis  

PubMed Central

Lymphangioleiomyomatosis (LAM) is a rare neoplastic disease, best characterized by the formation of proliferative nodules that express smooth muscle and melanocytic antigens within the lung parenchyma, leading to progressive destruction of lung tissue and function. The pathological basis of LAM is associated with Tuberous Sclerosis Complex (TSC), a multi-system disorder marked by low-grade tumors in the brain, kidneys, heart, eyes, lung and skin, arising from inherited or spontaneous germ-line mutations in either of the TSC1 or TSC2 genes. LAM can develop either in a patient with TSC (TSC-LAM) or spontaneously (S-LAM), and it is clear that the majority of LAM lesions of both forms are characterized by an inactivating mutation in either TSC1 or TSC2, as in TSC. Despite this genetic commonality, there is considerable heterogeneity in the tumor spectrum of TSC and LAM patients, the basis for which is currently unknown. There is extensive clinical evidence to suggest that the cell of origin for LAM, as well as many of the TSC-associated tumors, is a neural crest cell, a highly migratory cell type with extensive multi-lineage potential. Here we explore the hypothesis that the types of tumors that develop and the tissues that are affected in TSC and LAM are dictated by the developmental timing of TSC gene mutations, which determines the identities of the affected cell types and the size of downstream populations that acquire a mutation. We further discuss the evidence to support a neural crest origin for LAM and TSC tumors, and propose approaches for generating humanized models of TSC and LAM that will allow cell of origin theories to be experimentally tested. Identifying the cell of origin and developing appropriate humanized models is necessary to truly understand LAM and TSC pathology and to establish effective and long-lasting therapeutic approaches for these patients. PMID:25505789

Delaney, Sean P.; Julian, Lisa M.; Stanford, William L.

2014-01-01

462

Fases lquido-cristalinas anisotrpicas Fases lamelares preparao de lipossomas por  

E-print Network

#12;Mesofases em solventes orgânicos CTAB em (a) ­ água, (b) ­ glicerol, (c) ­ formamida, (d Uninvestigated area I DecanolC16 TAPA6000 Water II- nor hex, III- lam, IV ­ L2, V ­ inv hex, VI ­ inv rods II- nor hex, III- lam, IV ­ L2, V ­ inv rods I ­ nor hex, II ­lam III ­ L2 CTA+ + água + decanol : efeito

Loh, Watson

463

The above material may not be reproduced or distributed in any form without the express consent of Vidya Subramanian, ksubramanian@nycap.rr.com, 518-373-9037.  

E-print Network

kishoram Hatha chanooram hamsa viharam Pallavi 1. ; n s , M , ; gm , gsg | M ; ; ; ; G M D || Bha ja re go. gs n s, M , ; mdmm, gsg | M ; ; ; ; dmM gm D || Bha ja re go pa lam maa...... na N g s, nddM dm sgs D , s S S, n gs ndD|| Bha ja go pa lam bha ji ta ku che.........lam 1b. ; g m , dN S ; ; S | ; n n

Kalyanaraman, Shivkumar

464

PDGF enhances IRES-mediated translation of Laminin B1 by cytoplasmic accumulation of La during epithelial to mesenchymal transition.  

PubMed

The extracellular matrix protein Laminin B1 (LamB1) regulates tumor cell migration and invasion. Carcinoma cells acquire invasive properties by epithelial to mesenchymal transition (EMT), which is a fundamental step in dissemination of metastatic cells from the primary tumor. Recently, we showed that enhanced translation of LamB1 upon EMT of malignant hepatocytes is mediated by an internal ribosome entry site (IRES). We demonstrated that the IRES transacting factor La binds the minimal IRES motif and positively modulates IRES activity of LamB1. Here, we show that platelet-derived growth factor (PDGF) enhances IRES activity of LamB1 by the increasing cytoplasmic localization of La during EMT. Accordingly, cells expressing dominant negative PDGF receptor display reduced cytoplasmic accumulation of La and show no elevation of IRES activity or endogenous LamB1 levels after stimulation with PDGF. Furthermore, La-mediated regulation of LamB1 IRES activity predominantly depends on MAPK/ERK signaling downstream of PDGF. Notably, LamB1 expression is not significantly downregulated by the impairment of the translation initiation factor eIF4E. In vivo, knockdown of La associated with decreased LamB1 expression and reduced tumor growth. Together, these data suggest that PDGF is required for the cytoplasmic accumulation of La that triggers IRES-dependent translation of LamB1 during EMT. PMID:22904067

Petz, Michaela; Them, Nicole C C; Huber, Heidemarie; Mikulits, Wolfgang

2012-10-01

465

La enhances IRES-mediated translation of laminin B1 during malignant epithelial to mesenchymal transition.  

PubMed

The majority of transcripts that harbor an internal ribosome entry site (IRES) are involved in cancer development via corresponding proteins. A crucial event in tumor progression referred to as epithelial to mesenchymal transition (EMT) allows carcinoma cells to acquire invasive properties. The translational activation of the extracellular matrix component laminin B1 (LamB1) during EMT has been recently reported suggesting an IRES-mediated mechanism. In this study, the IRES activity of LamB1 was determined by independent bicistronic reporter assays. Strong evidences exclude an impact of cryptic promoter or splice sites on IRES-driven translation of LamB1. Furthermore, no other LamB1 mRNA species arising from alternative transcription start sites or polyadenylation signals were detected that account for its translational control. Mapping of the LamB1 5'-untranslated region (UTR) revealed the minimal LamB1 IRES motif between -293 and -1 upstream of the start codon. Notably, RNA affinity purification showed that the La protein interacts with the LamB1 IRES. This interaction and its regulation during EMT were confirmed by ribonucleoprotein immunoprecipitation. In addition, La was able to positively modulate LamB1 IRES translation. In summary, these data indicate that the LamB1 IRES is activated by binding to La which leads to translational upregulation during hepatocellular EMT. PMID:21896617

Petz, Michaela; Them, Nicole; Huber, Heidemarie; Beug, Hartmut; Mikulits, Wolfgang

2012-01-01

466

2013 Faculty Service Recognition Honorees 15 Years of Service  

E-print Network

, Communication Studies Cay Horstmann, Computer Science Lui Lam, Physics & Astronomy Jonathan Lovell, English, Economics Cynthia Rostankowski, Humanities Janie Scott, Music & Dance Bethany Shifflett, Kinesiology Carmen

Su, Xiao

467

Isolation of a distinct Mycobacterium tuberculosis mannose-capped lipoarabinomannan isoform responsible for recognition by CD1b-restricted T cells  

PubMed Central

Mannose-capped lipoarabinomannan (ManLAM) is a complex lipoglycan abundantly present in the Mycobacterium tuberculosis cell envelope. Many biological properties have been ascribed to ManLAM, from directly interacting with the host and participating in the intracellular survival of M. tuberculosis, to triggering innate and adaptive immune responses, including the activation of CD1b-restricted T cells. Due to its structural complexity, ManLAM is considered a heterogeneous population of molecules which may explain its different biological properties. The presence of various modifications such as fatty acids, succinates, lactates, phosphoinositides and methylthioxylose in ManLAM have proven to correlate directly with its biological activity and may potentially be involved in the interactions between CD1b and the T cell population. To further delineate the specific ManLAM epitopes involved in CD1b-restricted T cell recognition, and their potential roles in mediating immune responses in M. tuberculosis infection, we established a method to resolve ManLAM into eight different isoforms based on their different isoelectric values. Our results show that a ManLAM isoform with an isoelectric value of 5.8 was the most potent in stimulating the production of interferon-? in different CD1b-restricted T-cell lines. Compositional analyses of these isoforms of ManLAM revealed a direct relationship between the overall charge of the ManLAM molecule and its capacity to be presented to T cells via the CD1 compartment. PMID:22534567

Torrelles, Jordi B; Sieling, Peter A; Zhang, Nannan; Keen, Mark A; McNeil, Michael R; Belisle, John T; Modlin, Robert L; Brennan, Patrick J; Chatterjee, Delphi

2012-01-01

468

Animation of Flood Potential from Two Australian Tropical Cyclones - Duration: 0:06.  

NASA Video Gallery

Merged precipitation data from NASA-JAXA's Tropical Rainfall Measuring Mission (TRMM) and other satellites was used to calculate flood potential withrainfall from Tropical Cyclone Lam and Tropical ...

469

Partenaires : la Haute Ecole Charlemagne, la Haute Ecole de la Province de Lige, la Haute Ecole de la Ville de Lige, la Haute Ecole Libre Mosane, la Haute Ecole Robert Schuman et l'Universit de Lige.  

E-print Network

plantes psychotropes sont à l'origine des drogues les plus consommées actuellement: - Pavot (Papaver somniferum L.); - Chanvre (Cannabis sativa L.); - Coca (Erythrocylum coca Lam). Les aspects historiques

Liège, Université de

470

Abstract While several recent studies have described changes in microbial communities asso-  

E-print Network

, Centaurea maculosa Lam. (spotted knapweed), and in adjacent native grassland sites. Our results indicate. (Asteraceae, spotted knapweed), specifically, can cause shifts in the composition of soil bacterial

Rilli, Matthias C.

471

Anticonvulsant activity of berberine, an isoquinoline alkaloid in mice  

Microsoft Academic Search

Berberine, an isoquinoline alkaloid is reported to modulate several neurotransmitter systems like N-methyl-D-aspartate, nitric oxide and serotonin, which modulate convulsions. In addition, it is suggested that Berberis vulgaris may be useful in treatment of convulsion and epilepsy. Therefore, the present study investigated the effects of berberine in pentylenetetrazole, maximal electroshock (MES) and kainic acid (KA)-induced convulsions. The latency for development

Pravinkumar Bhutada; Yogita Mundhada; Kuldeep Bansod; Pankaj Dixit; Sudhir Umathe; Dharmendra Mundhada

2010-01-01