Sample records for berberis buxifolia lam

  1. Berberis sp. (Cultivated) 2 

    E-print Network

    James R. Manhart

    2011-08-10

    Factors Influencing Leaf Dynamics Random factors Predictable factors Functional Groupings 26 26 27 29 ik TABLE OF CONTENTS (continued) Shrub Cluster Influences IV NUTRIENT INPUTS VIA PRECIPITATION, THROUGHFALL AND STEMFLOW Introduction Page... Deciduous Coriaceous Deciduous/ Goriacecus everrIreen Smooth Tree oz' ~; 1, 3 rounded crown Smooth Shrub or tree 1, 3 Smooth Small tree 1, 3 or large shrub 1, 3 Spiny ha~ (~Mlt' ll'da Il Agarito (Berberis trifoliolata Moric. ) Deciduous...

  2. The Leaf Oils of Severinia buxifolia (Poir.) Tenore

    Microsoft Academic Search

    Rainer W. Scora; Mukhtar Ahmed

    1994-01-01

    The citrus relative Severinia buxifolia is tolerant to boron and salt, and is resistant to nematode infection, thus making it a potential rootstock for cultivated citrus. Its essential leaf oils were analyzed by GC and GC\\/MS, and 59 of the 73 separated components were identified by their MS spectra and retention indices. The MS data are given for the remaining

  3. Systematics and phylogeographic studies of Berberis L. (Berberidaceae) in the Nepal Himalaya 

    E-print Network

    Adhikari, Bhaskar

    2010-01-01

    Berberis L. contains more than 500 species, is the largest genus in the Berberidaceae, and is now recognised to comprise Berberis s.s. with simple leaves and compoundleaved species formally ascribed to Mahonia. Because ...

  4. Structure, diversity, and regeneration potential of Monotheca buxifolia (Falc.) A. DC. dominated forests of Lower Dir District, Pakistan

    Microsoft Academic Search

    Nasrullah Khan; Moinuddin Ahmed; Syed Shahid Shaukat; Muhammad Wahab; Muhammad Faheem Siddiqui

    2011-01-01

    This study reports on the multivariate analysis of the vegetation of Hindukush Range in Pakistan, concentrating on the structure\\u000a and regeneration potential of Monotheca buxifolia and associated tree species. Twenty stands at different locations in the Dir District of the Hindukush Range in Pakistan\\u000a were chosen for the study. A point centered quarter method for trees and 5m × 5m

  5. LAM/MPI

    NSDL National Science Digital Library

    The Llamas

    LAM/MPI is a high quality implementation of the Message Passing Interface (MPI) Standard. LAM/MPI provides high performance on a variety of platforms, from small off-the-shelf single CPU clusters to large SMP machines with high speed networks, even in heterogenous environments. In addition to high performance, LAM provides a number of usability features key to developing large scale MPI applications.

  6. Cytotoxic Activity of Methanolic Extract of Berberis aristata DC on Colon Cancer

    Microsoft Academic Search

    Saumya Das; Papiya Mitra Mazumder

    2009-01-01

    2 Abstract: Natural products represent a reservoir of diverse templates and are being tapped to outsource novel anticancer agents. Berberis aristata DC (Fam: Berberidaceae) has been reported to be useful for the treatment of inflammation, cuts, wounds, eye and skin diseases, diarrhea, ulcers, cancers. In the present study, the methanolic extract of the stems of Berberis aristata, was investigated against

  7. Phyto-chemical and pharmacological applications of Berberis aristata.

    PubMed

    Potdar, Dipti; Hirwani, R R; Dhulap, Sivakami

    2012-07-01

    In the recent years, the interest and research in medicinal plants have increased in a great deal. Ayurvedic medicines and formulations developed from ancient Indian herbal systems are renowned for their various important applications. Berberis aristata - an Indian medicinal plant, which belongs to the family Berberidaceae is an ayurvedic herb used since ancient times. It is also known as Indian berberi, Daruharidra, Daruhaldi, Darvi and Chitra. The plant is useful as anti-pyretic, anti-bacterial, anti-microbial, anti-hepatotoxic, anti-hyperglycaemic, anti-cancer, anti-oxidant and anti-lipidemic agent. B. aristata extracts and its formulations are also useful in the treatment of diarrhoea, haemorrhoids, gynaecological disorders, HIV-AIDS, osteoporosis, diabetes, eye and ear infections, wound healing, jaundice, skin diseases and malarial fever. This review aims to highlight the ethnobotany, pharmacognosy and pharmacological uses of B. aristata which will give insights in developing potentially new bioactives from the plant scaffolds. This review will also highlight the patenting trends, the new compositions developed using the actives from B. aristata and the different assignees involved in filing patents. PMID:22808523

  8. Identification and characterization of microsatellites from calafate (Berberis microphylla, Berberidaceae)1

    PubMed Central

    Varas, Benjamín; Castro, María H.; Rodriguez, Roberto; von Baer, Dietrich; Mardones, Claudia; Hinrichsen, Patricio

    2013-01-01

    • Premise of the study: Southern barberry or calafate (Berberis microphylla) is a shrub species endemic to the Patagonian region of South America that is used for human consumption. The fruit is very rich in vitamin C and anthocyanins and has a very high antioxidant capacity. There have been only a few genetic studies of this and other closely related species. • Methods and Results: Here we present the first 18 microsatellite markers of B. microphylla that were characterized using 66 accessions of calafate from Patagonia. On average, they had 7.6 alleles per marker, with an expected heterozygosity of 0.688. The informativeness of these markers was also evaluated in another 15 Berberis species, including most of the native and endemic Chilean species. • Conclusions: The results confirm that these new simple sequence repeat markers are very polymorphic and potentially useful in genetic studies in any species of the genus Berberis. PMID:25202561

  9. Flavonols, alkaloids, and antioxidant capacity of edible wild berberis species from patagonia.

    PubMed

    Ruiz, Antonieta; Zapata, Moises; Sabando, Constanza; Bustamante, Luis; von Baer, Dietrich; Vergara, Carola; Mardones, Claudia

    2014-12-24

    There are 20 species of the Berberidaceae family described in Chile, whose fruits are edible and show high anthocyanin and hydroxycinnamic acid levels. Berberis microphylla G. Forst, commonly known as calafate, is the most extensively distributed. Flavonols and alkaloids in seed, pulp, skin, and whole calafate berry extracts and other Berberis were studied using HPLC-DAD-ESI-MS/MS and HPLC with fluorescence detector. Berry samples from different locations in Chilean Patagonia, including different phenological stages, were systematically addressed. Results were compared with other organs of the plant and with other Berberis species. Total flavonol concentration in calafate (n = 65) was 1.33 ± 0.54 ?mol/g. Glycosyl metabolites of quercetin and isorhamnetin were the most abundant. Similar profiles were observed in calafate from distinct locations, but important differences were observed for the other edible Berberis species. Calafate pulp and skin have higher flavonol concentrations than seeds, and the maturation process reduced its levels. TEACCUPRAC and TEACABTS of whole calafate extracts and fractions are also explored. Finally, only berberine was detected in the fruit (0.001%), mainly in seeds. Results contribute to the promotion of this berry as a superfruit from Patagonia. PMID:25495577

  10. Ploidy Levels and Genome Sizes of Berberis L. and Mahonia Nutt. Species, Hybrids, and Cultivars

    Microsoft Academic Search

    Todd J. Rounsaville; Thomas G. Ranney

    2010-01-01

    An extensive survey of genome sizes and ploidy levels was conducted for a diverse collection of Berberis and Mahonia taxa (Berberidaceae). Propidium iodide flow cytometric analysis was conducted using Pisum sativum L. 'Ctirad' (2C DNA = 8.76 pg) as an internal standard to determine genome sizes. Mean 1CX genome sizes varied between the two Mahonia subgenera (Occidentales = 1.17 ±

  11. Blue sensitizers for solar cells: Natural dyes from Calafate and Jaboticaba

    Microsoft Academic Search

    André Sarto Polo; Neyde Yukie Murakami Iha

    2006-01-01

    Blue-violet anthocyanins from Jaboticaba (Myrtus cauliflora Mart) and Calafate (Berberies buxifolia Lam) were employed as TiO2 dye-sensitizers. Solar cells sensitized by Jaboticaba extracts achieved up to Jsc=9.0mAcm?2, Voc=0.59V, Pmax=1.9mWcm?2 and ff=0.54, while for Calafate sensitized cells the values determined were up to Jsc=6.2mAcm?2, Voc=0.47V, Pmax=1.1mWcm?2 and ff=0.36. Other natural dyes were evaluated without significant photocurrent, demonstrating that only selected extracts

  12. Characterisation and preliminary bioactivity determination of Berberis boliviana Lechler fruit anthocyanins

    Microsoft Academic Search

    Carla Del Carpio Jiménez; Carlos Serrano Flores; Jian He; Quingguo Tian; Steven J. Schwartz; M. Mónica Giusti

    2011-01-01

    Berberis boliviana Lechler is a member of the Berberidaceae family that has a small edible red-purple berry. The plant is native to the Peruvian Andes and contains high amounts of anthocyanin pigments. The monomeric anthocyanin content, determined by a pH-differential method, was 7\\/100g of seedless berries. Pigments were characterised by HPLC coupled to a photodiode array (PDA) and mass spectrophotometer

  13. A new isoquinoline alkaloid with anti-microbial properties from Berberis jaeschkeana Schneid. var. jaeschkeana.

    PubMed

    Alamzeb, Muhammad; Khan, M Rafiullah; Mamoon-Ur-Rashid; Ali, Saqib; Khan, Ashfaq Ahmad

    2015-01-01

    One new isoquinoline alkaloid named berberidione (1) along with four new source alkaloids berberine (2), palmatine (3), jatrorrhizine (4) and chondrofoline (5) and three new source non-alkaloids syringic acid (6), ?-sitosterol (7) and stigmasterol (8) was isolated and characterised from different fractions of Berberis jaeschkeana Schneid var. jaeschkeana. All the structures were determined from 1D and 2D spectroscopic data. Crude extract, sub-fractions and isolated compounds showed excellent anti-microbial properties. The toxicity level for the alkaloids was found to be very low on THP-1 cells. PMID:25424893

  14. Century-old Mystery of Puccinia striiformis Life History Solved with the Identification of Berberis as an Alternate Host

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The life history of Puccinia striiformis remains a mystery because the alternate host has never been found. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P...

  15. The Anti-inflammatory Effects of Curcuma longa and Berberis aristata in Endotoxin-Induced Uveitis in Rabbits

    Microsoft Academic Search

    Suresh Kumar Gupta; Renu Agarwal; Sushma Srivastava; Puneet Agarwal; Shyam Sunder Agrawal; Rohit Saxena; Niranjan Galpalli

    PURPOSE. To investigate the anti-inflammatory effect of topical application of Curcuma longa (C. longa) and Berberis aristata (B. aristata) aqueous extracts on experimental uveitis in the rabbit. METHODS. Anterior uveitis was induced in rabbits by intravitreal injection of lipopolysaccharide from Escherichia coli after pre- treatment with C. longa and B. aristata aqueous extracts. Subsequently, the anti-inflammatory activity of C. longa

  16. A component architecture for LAM\\/MPI

    Microsoft Academic Search

    Jeffrey M. Squyres; Andrew Lumsdaine

    2003-01-01

    To better manage the ever increasing complexity of LAM\\/MPI, we have created a lightweight component architecture for it that is specif- ically designed for high-performance message passing. This paper de- scribes the basic design of the component architecture, as well as some of the particular component instances that constitute the latest release of LAM\\/MPI. Performance comparisons against the previous, mono-

  17. Polyphenols and antioxidant activity of calafate ( Berberis microphylla ) fruits and other native berries from Southern Chile.

    PubMed

    Ruiz, Antonieta; Hermosín-Gutiérrez, Isidro; Mardones, Claudia; Vergara, Carola; Herlitz, Erika; Vega, Mario; Dorau, Carolin; Winterhalter, Peter; von Baer, Dietrich

    2010-05-26

    Calafate ( Berberis microphylla ) is a native berry grown in the Patagonian area of Chile and Argentina. In the present study the phenolic composition and antioxidant activity of its fruits were studied and also compared with data obtained for other berry fruits from southern Chile including maqui ( Aristotelia chilensis ) and murtilla ( Ugni molinae ). Polyphenolic compounds in calafate fruit were essentially present in glycosylated form, 3-glucoside conjugates being the most abundant anthocyanins. The anthocyanin content in calafate berries (17.81 +/- 0.98 micromol g(-1)) and flavonol level (0.16 +/- 0.01 micromol g(-1)) are comparable with those found in maqui (17.88 +/- 1.15 and 0.12 +/- 0.01 micromol g(-1), respectively); however, maqui shows lower flavan-3-ol concentration than calafate (0.11 +/- 0.01 and 0.24 +/- 0.03 micromol g(-1), respectively). Maqui and calafate show high antioxidant activity, which correlates highly with total polyphenol content and with anthocyanin concentration. PMID:20438111

  18. Chapter 7.11 Lam6 Oxide Rainbow Etcher

    E-print Network

    Healy, Kevin Edward

    Chapter 7.11 Lam6 Oxide Rainbow Etcher (lam6) (584) 1.0 Equipment Purpose 1.1 Lam6 is an automatic, cassette-to-cassette, plasma etcher (Rainbow 4520). This tool is currently dedicated to etching silicon and severely impact consequent etch processes. 3.0 Applicable Documents 3.1 Rainbow 4520 Series System

  19. Effects of Zataria multiflora and Carum carvi essential oils and hydroalcoholic extracts of Passiflora incarnata, Berberis integerrima and Crocus sativus on rat isolated uterus contractions

    Microsoft Academic Search

    Hassan Sadraei; Alireza Ghannadi; Maryam Takei-bavani

    2003-01-01

    Spontaneous abortion can be a problem in pregnancy. Consumption of some plant material might be a cause, while other plant materials are being used in folk medicine for treatment of this condition. The aim of this study was to look for effects of essential oils of Zataria multiflora and Carum carvi and hydroalcoholic extracts of Passiflora incarnata, Berberies integerrima and

  20. Antihyperglycemic and Antihyperlipidemic Effects of Fruit Aqueous Extract of Berberis integerrima Bge. in Streptozotocin-induced Diabetic Rats.

    PubMed

    Ashraf, Hossein; Heidari, Reza; Nejati, Vahid

    2014-01-01

    Use of medicinal plants for attenuation of hyperglycemia and restoration of lipids disorder to normal level is clinically very important. The aim of present study was to evaluate the effects of Berberis integerrima Bge. fruit aqueous extract (BIFAE) on blood glucose and lipid profile in streptozotocin (STZ) - induced diabetic rats. The STZ-induced diabetic rats were treated by fruit aqueous extract of Berberis integerrima Bge. at doses (250 and 500 mg/Kg bw) and glibenclamide (0.6 mg/Kg bw) for 42 days by gavage. Blood glucose levels and body weights of rats were measured on weeks 0, 2, 4 and 6. Total lipid levels were determined in normal and STZ-induced diabetic rats after administration of the BIFAE and glibenclamide for 42 days. STZ-induced diabetic rats showed a significant (P<0.001) increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C) while body weight and high density lipoprotein HDL-cholesterolan (HDL-C) were significantly(P<0.001) decreased compared to normal rats. Daily administration of BIFAE did not possess the hypoglycemic and hypolipidaemic activity in STZ- diabetic rats during 6-week treatment period. Results indicate the usage of BIFAE in traditional medicine for the treatment of diabetes may need more investigation. PMID:25587320

  1. Legitimate seed dispersal by lizards in an alpine habitat: The case of Berberis empetrifolia (Berberidaceae) dispersed by Liolaemus belii (Tropiduridae)

    NASA Astrophysics Data System (ADS)

    Celedón-Neghme, Constanza; San Martin, Leonardo A.; Victoriano, Pedro F.; Cavieres, Lohengrin A.

    2008-05-01

    In this study we determined the effect of seed passage through Liolaemus bellii lizard digestive tracts on germination of fleshy-fruited Andean shrub species Berberis empetrifolia (Berberidaceae), and evaluated the effect of this passage on seed coat characteristics. In addition, we assessed the spatial patterns of fecal deposition by lizards onto various microhabitats available in the Andean environments of central Chile. The germination rate and the final percentage of lizard-ingested B. empetrifolia seeds was greater than control seeds. Comparing photographs and seed coat histological cuts, we suggest that the cutine wax present on seed coats from lizard-ingested seeds was probably removed by abrasion inside the lizards' digestive tract. Sixty-two percent of the lizard's feces was deposited on bare soil near rocks commonly inhabited by lizards. However, this microhabitat represents only 29% of the available ground cover at the study site. By enhancing seed germination and depositing seeds onto potential safe sites for recruitment, the lizard Liolaemus bellii is acting, at least qualitatively, as an effective disperser of Berberis empetrifolia.

  2. Copyright 2011 Jennifer Lam-Anh Gosselin

    E-print Network

    Washington at Seattle, University of

    -like pattern in the survival curves of juvenile hatchery rainbow trout (Oncorhynchus mykiss). In Chapter 4 Survival: Examples from a model species (Poecilia reticulata) and salmonid species (Oncorhynchus spp: Examples from a model species (Poecilia reticulata) and salmonid species (Oncorhynchus spp.) Jennifer Lam

  3. “Getting stuck with LAM”: patients perspectives on living with Lymphangioleiomyomatosis

    PubMed Central

    2014-01-01

    Background Lymphangioleiomyomatosis (LAM) is a rare, progressive lung disease that affects almost exclusively women and is most often diagnosed before menopause. The main symptom of LAM is shortness of breath. LAM patients’ perceptions of how the disease impacts their lives is largely unknown, but such information could be useful to generate patient reported outcome measures for use in drug trials (or other research studies) and to formulate interventions aimed at easing the burdens LAM imposes on patients. Objective To capture patients’ perceptions of how LAM affects their lives. Methods We used reflexive team analysis to analyze transcripts from semi-structured focus groups conducted with LAM patients at LAMposium 2013. We sought to determine what patients perceive as the primary symptoms of LAM and how the disease affects them in their daily lives. Results The 37 participants described seven primary symptoms of LAM and five common psychological experiences from living with the disease. Shortness of breath and low energy (or fatigue) dominated the symptomatic picture; cough, sensations in the chest, difficulty sleeping, gastrointestinal issues, and mild cognitive difficulties were less common. The common psychological experiences participants reported included frustration, worry, loss of identity, embarrassment, and in some participants, a healthy defiance against the disease. Conclusions Patients perceive the physical symptoms from LAM to be intrusive and limiting. Women living with LAM are frustrated by their physical limitations, and they worry about what the future will be like if the disease progresses. Therapeutic interventions should take aim at improving these perceptions. PMID:24885591

  4. Lymphangioleiomyomatosis (LAM): Molecular insights lead to targeted therapies

    PubMed Central

    Glasgow, Connie G.; Steagall, Wendy K.; Taveira-DaSilva, Angelo; Pacheco-Rodriguez, Gustavo; Cai, Xiong; El-Chemaly, Souheil; Moses, Marsha; Darling, Thomas; Moss, Joel

    2011-01-01

    Summary LAM is a rare lung disease, found primarily in women of childbearing age, characterized by cystic lung destruction and abdominal tumors (e.g., renal angiomyolipoma, lymphangioleiomyoma). The disease results from proliferation of a neoplastic cell, termed the LAM cell, which has mutations in either of the tuberous sclerosis complex (TSC) 1 or TSC2 genes. Molecular phenotyping of LAM patients resulted in the identification of therapeutic targets for drug trials. Loss of TSC gene function leads to activation of mammalian target of rapamycin (mTOR), and thereby, effects on cell size and number. The involvement of mTOR in LAM pathogenesis is the basis for initiation of therapeutic trials of mTOR inhibitors (e.g., sirolimus). Occurrence of LAM essentially entirely in women is consistent with the hypothesis that anti-estrogen agents might prevent disease progression (e.g., gonadotropin-releasing hormone analogues). Levels of urinary matrix metalloproteinases (MMPs) were elevated in LAM patients, and MMPs were found in LAM lung nodules. In part because of these observations, effects of doxycycline, an anti-MMP, and anti-angiogenic agent, are under investigation. The metastatic properties of LAM cells offer additional potential for targets. Thus, insights into the molecular and biological properties of LAM cells and molecular phenotyping of patients with LAM have led to clinical trials of targeted therapies. Funded by the Intramural Research Program, NIH/NHLBI PMID:20630348

  5. Jackfruit (Artocarpus heterophyllus Lam.) Diversity in Bangladesh: Land Use and Artificial Selection1

    E-print Network

    Fant, Jeremie

    Jackfruit (Artocarpus heterophyllus Lam.) Diversity in Bangladesh: Land Use and Artificial *Corresponding author; e-mail: nzerega@chicagobotanic.org Jackfruit (Artocarpus heterophyllus Lam.) Diversity of diversity for jackfruit (Artocarpus heterophyllus Lam.) and is expected to harbor important genetic

  6. A survey of fungal plant pathogens associated with weed infestations of barberry ( Berberis spp.) in New Zealand and their biocontrol potential

    Microsoft Academic Search

    N. W. Waipara; L. A. Smith; A. F. Gianotti; J. P. Wilkie; C. J. Winks; E. H. C. McKenzie

    2005-01-01

    Since the introduction and subsequent naturalisation of five species of Berberis into New Zealand, two species, B. glaucocarpa and B. darwinii have become aggressive invaders of both agricultural and native ecosystems throughout many regions. Both are now targets\\u000a for a biological control program. A survey for pathogens to be used as potential classical or inundative biocontrol agents\\u000a was initiated on

  7. Quantitative determination of isoquinoline alkaloids and chlorogenic acid in Berberis species using ultra high performance liquid chromatography with hybrid triple quadrupole linear ion trap mass spectrometry.

    PubMed

    Singh, Awantika; Bajpai, Vikas; Kumar, Sunil; Arya, Kamal Ram; Sharma, Kulwant Rai; Kumar, Brijesh

    2015-06-01

    Berberis species are well known and used extensively as medicinal plants in traditional medicine. They have many medicinal values attributable to the presence of alkaloids having different pharmacological activities. In this study, a method was developed and validated as per international conference on harmonization guidelines using ultra high performance liquid chromatography with hybrid triple quadrupole-linear ion trap mass spectrometry operated in the multiple reaction monitoring mode for nine bioactive compounds, including protoberberine alkaloids, aporphine alkaloids and chlorogenic acid. This method was applied in different plant parts of eight Berberis species to determine variations in content of nine bioactive compounds. The separation was achieved on an ACQUITY UPLC CSH™ C18 column using a gradient mobile phase at flow rate 0.3 mL/min. Calibration curves for all the nine analytes provided optimum linear detector response (with R(2) ?0.9989) over the concentration range of 0.5-1000 ng/mL. The precision and accuracy were within RSDs ?2.4 and ?2.3%, respectively. The results indicated significant variation in the total contents of the nine compounds in Berberis species. PMID:25847792

  8. Rehabilitation program faculty mem-bers Chow Lam, Patrick Corrigan,

    E-print Network

    Heller, Barbara

    Rehabilitation program faculty mem- bers Chow Lam, Patrick Corrigan, and Jon Larson, and Institute in rehabilitation and education. There are very few universities in China that offer studies on rehabili- tation psychology, and Lam believes IIT's MOU with CAS and his efforts in training future rehabilitation pro

  9. Comparative Study of Berberis vulgaris Fruit Extract and Berberine Chloride Effects on Acetic Acid-Induced Colitis in Rats

    PubMed Central

    Minaiyan, Mohsen; Ghannadi, Alireza; Mahzouni, Parvin; Jaffari-Shirazi, Elham

    2011-01-01

    Antioxidant and immunomodulatory effects of anthocyanins are abundant in berberry fruits suggesting that they may have beneficial effects on inflammatory bowel diseases (IBD). The present study was carried out to investigate the anti-colitic effect of Berberis vulgaris fruit extract (BFE) compared to berberine chloride (BEC) and corticosteroids using an animal model of acetic acid induced experimental colitis. BFE with three different doses (375, 750, and 1500 mg/Kg) was administered orally or rectally prior to ulcer induction. BEC (10 mg/Kg), prednisolone (5 mg/Kg), hydrocortisone acetate enema (20 mg/Kg) and normal saline (5 mL/Kg) were considered as respective controls. The tissue was assessed macroscopically for damage scores, area, index and weight/length ratio. They were also examined histopathologically for inflammation extent and severity, crypt damage, invasion involvement and total colitis index. Results indicated that greater doses of oral BFE (750, 1500 mg/Kg) as well as BEC (10 mg/Kg) were effective to protect against colonic damage. By rectal pretreatment, the extract was only effective to diminish the ulcer index and the efficacy was not significant for mucosal inflammation parameters. In conclusion BFE, which is nearly devoid of berberine, was effective to protect against colitis and this might be attributed to its anthocyanin constituents. PMID:24363687

  10. UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kelsie Priest, Lahiru Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam

    E-print Network

    Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam UBC Botanical Garden Redevelopment of a project/report". #12;UBC Botanical Garden Redevelopment Detailed Design Report Prepared for UBC CIVIL 446 and UBC Botanical Garden Prepared by CIVIL 446 - Group One Mike Kai Hin Lam # Kelsie Priest # Lahiru

  11. Computer-Aided Grading of Lymphangioleiomyomatosis (LAM) using HRCT

    PubMed Central

    Yao, Jianhua; Avila, Nilo; Dwyer, Andrew; Taveira-DaSilva, Angelo M.; Hathaway, Olanda M.; Moss, Joel

    2010-01-01

    Lymphangioleiomyomatosis (LAM) is a multisystem disorder associated with proliferation of smooth muscle-like cells, which leads to destruction of lung parenchyma. Subjective grading of LAM on HRCT is imprecise and can be arduous especially in cases with severe involvement. We propose a computer-aided evaluation system that grades LAM involvement based on analysis of lung texture patterns. A committee of support vector machines is employed for classification. The system was tested on 36 patients. The computer grade demonstrates good correlation with subjective radiologist grade (R=0.91, p<0.0001) and pulmonary functional tests (R=0.85, p<0.0001). The grade also provides precise progression assessment of disease over time. PMID:21625320

  12. Lam6 Regulates the Extent of Contacts between Organelles.

    PubMed

    Elbaz-Alon, Yael; Eisenberg-Bord, Michal; Shinder, Vera; Stiller, Sebastian Berthold; Shimoni, Eyal; Wiedemann, Nils; Geiger, Tamar; Schuldiner, Maya

    2015-07-01

    Communication between organelles is crucial for eukaryotic cells to function as one coherent unit. An important means of communication is through membrane contact sites, where two organelles come into close proximity allowing the transport of lipids and small solutes between them. Contact sites are dynamic in size and can change in response to environmental or cellular stimuli; however, how this is regulated has been unclear. Here, we show that Saccharomyces cerevisiae Lam6 resides in several central contact sites: ERMES (ER/mitochondria encounter structure), vCLAMP (vacuole and mitochondria patch), and NVJ (nuclear vacuolar junction). We show that Lam6 is sufficient for expansion of contact sites under physiological conditions and necessary for coordination of contact site size. Given that Lam6 is part of a large protein family and is conserved in vertebrates, our work opens avenues for investigating the underlying principles of organelle communication. PMID:26119743

  13. In vitro biological assessment of berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects

    PubMed Central

    2013-01-01

    Background Berberis vulgaris is a well known plant with traditional herbal medical history. The aims of this study was to bioscreen and compare the in vitro biological activity (antioxidant, cholinergic, antidaibetic and the anticancer) of barberry crude extract and berberine active compound. Methods The effect of B. vulgaris extract and berberine chloride on cellular thiobarbituric acid reactive species (TBARS) formation, diphenyle–?-picrylhydrazyl (DPPH) oxidation, cellular nitric oxide (NO) radical scavenging capability, superoxide dismutase (SOD), glutathione peroxidase (GPx), acetylcholinesterase (AChE) and ?-gulcosidase activities were spectrophotometrically determined. On the other hand, the effect of extract and berberine as anticancer was estimated on three different cell lines which were MCF-7, HepG-2, and Caco-2 cells by using neutral red uptake assay which compared with control normal cells (PBMC). Results Our results showed that barberry crude extract contains 0.6 mg berberine/mg crude extract. Barberry extract showed potent antioxidative capacity through decreasing TBARS, NO and the oxidation of DPPH that associated with GPx and SOD hyperactivation. Inhibitory effect of berberis crude extract on ?-glucosidase was more potent than that of berberine chloride, while both had the same AChE inhibitory effect. Besides, different concentrations of both berberine chloride and barberry ethanolic extract showed to have no growth inhibitory effect on normal blood cells (PBMC). Otherwise, both berberine chloride and barberry ethanolic extract showed to have inhibitory effect on the growth of breast, liver and colon cancer cell lines (MCF7, HepG2 and CACO-2, respectively) at different incubation times starting from 24 hrs up to 72 hrs and the inhibitory effect increased with time in a dose dependant manner. Conclusion This work demonstrates the potential of the barberry crude extract and its active alkaloid, berberine, on suppressing lipid peroxidation, suggesting a promising use in the treatment of hepatic oxidative stress, Alzheimer and idiopathic male factor infertility. Beside, berberis vulgaris ethanolic extract is safe non-toxic extract as it was not inhibit the growth of PBMC that can induce cancer cell death that could return to its powerful antioxidant activity. PMID:24007270

  14. UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kelsie Priest, Lahiru Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam

    E-print Network

    Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam UBC Botanical Garden Redevelopment CIVLGarden Redevelopment Prepared for UBC CIVIL 445 and UBC Botanical Garden Prepared by CIVIL 445 Group Botanical Garden Redevelopment" is submitted by Group 1 in response to request by UBC Botanical Garden

  15. Rapid screening for the adulterants of Berberis aristata using direct analysis in real-time mass spectrometry and principal component analysis for discrimination.

    PubMed

    Bajpai, Vikas; Singh, Awantika; Arya, Kamal Ram; Srivastava, Mukesh; Kumar, Brijesh

    2015-06-01

    Adulteration or substitution of commercial Berberis aristata and its herbal products with inferior-quality substituents is very common. Metabolic profiling of B. aristata, along with its common adulterants/contaminants/substituents such as B. asiatica, Mahonia borealis and Coscinium fenestratum, was rapidly carried out using direct analysis in real-time mass spectrometry (DART MS) to generate the chemical fingerprints for the differentiation of these species. Phytochemical analysis showed the presence of mainly alkaloids. The identified alkaloids were berberrubine, berberine, jatrorrhizine, ketoberberine, palmatine, dihydropalmatine or 7,8-dihydro-8-hydroxyberberine, berbamine and pakistanamine. Berberine, which was mainly reported from the root and stem bark of B. aristata, was also identified in the leaf along with chlorogenic acid. The DART MS data have been subjected to principal component analysis (PCA). The resulting score plots showed clustering and clear differentiation of the species and plant parts. It is thus apparent that the technique of DART MS followed by PCA is a quick and reliable method for the direct profiling of B. aristata and its adulterant plants and plant parts. The study reports the rapid analytical method to identify the possibility of illegal adulteration/contamination/substitution in potential plant materials and herbal extracts. PMID:25739096

  16. In vitro and in vivo antimutagenic effects of DIG, a herbal preparation of Berberis vulgaris, Taraxacum officinale and Arctium lappa, against mitomycin C.

    PubMed

    Di Giorgio, C; Boyer, L; De Meo, M; Laurant, C; Elias, R; Ollivier, E

    2015-07-01

    DIG, a liquid herbal preparation made from a mixture of diluted mother tinctures of Berberis vulgaris, Taraxacum officinale and Arctium lappa, was assessed for its antimutagenic properties against mitomycin C. The micronucleus assay on Chinese hamster ovary (CHO)-K1 cells was used to evaluate the in vitro anticlastogenic activity of DIG compared to those of separately diluted mother tinctures. The micronucleus assay was performed on mouse erythrocytes and the comet assay was performed on mouse liver, kidney, lung, brain and testicles to assess the protective effects of DIG (0.2 and 2 % at libitum) against an intraperitoneal injection of mitomycin C (1 mg Kg(-1)) in mice. DIG exerted a powerful anticlastogenic activity, under both pretreatment and simultaneous treatment conditions as assessed by the micronucleus assay in CHO-K1 cells. Its protective activity was greater than that observed for each mother tincture. DIG reduced micronuclei levels in mouse erythrocytes and suppressed >80 % of DNA strand breaks in the liver, kidney, lung, brain and testicles of mice exposed to mitomycin C. PMID:25666712

  17. Using Learning Activity Management Systems (LAMS) with pre-service secondary teachers: An authentic task

    Microsoft Academic Search

    Chris Campbell; Leanne Cameron

    2009-01-01

    Within an authentic learning framework, second year pre-service teachers were introduced to LAMS (the Learning Activity Management System) as part of one of the information and communication technology (ICT) units they are required to complete as part of their course. Using case study methodology, the students returned some interesting results: LAMS helped the students plan all aspects of their lesson

  18. Time domain modelling of room acoustics Y.W. Lam and J. Hargreaves

    E-print Network

    Paris-Sud XI, Université de

    Time domain modelling of room acoustics Y.W. Lam and J. Hargreaves University of Salford, School of Computing, Science and Engineering, M5 4WT Salford, UK y.w.lam@salford.ac.uk Proceedings of the Acoustics advantages for room acoustics investigations. It directly generates the efficient for broadband calculations

  19. Essential oil changes in capsules of carqueja (Baccharis genistelloides (Lam.) Pers.)

    Microsoft Academic Search

    R. G. Rodrigues-das-Dôres

    Essential oil changes in capsules of carqueja (Baccharis genistelloides (Lam.) Pers.). Essential oil changes in capsules (PEC) and rude powder (PBC) of dry powder from 'carqueja' Baccharis genistelloides (Lam.) Pers, Asteraceae, was evaluated along 320 days of storage. The powder was encapsulated in colorless gelatinous capsules, number zero, stored in white bottles, colorless the rude powder was maintained in amber

  20. A Job Pause Service under LAM/MPI+BLCR for Transparent Fault Tolerance

    E-print Network

    Engelmann, Christian

    A Job Pause Service under LAM/MPI+BLCR for Transparent Fault Tolerance Chao Wang, Frank Mueller; 2 Outline Problem vs. Our Solution Overview of LAM/MPI and BLCR Our Design and Implementation becomes shorter Extensions to MPI for FT exist but... MPI widely accepted in scientific computing

  1. Hydrocarbon composition of crude oil from Lam Bank

    SciTech Connect

    Samedova, F.I.; Agaeva, R.M.; Alieva, F.Z.; Valiev, M.A.

    1987-07-01

    The authors discuss the crude oil from a new offshore field called the Lam Bank in the Caspian Sea. A segregated commercial crude was distilled and the distillation data is shown. In order to determine the content of n-paraffins, the naphthenic-paraffinic part of the narrow cuts was subjected to adsorptive separation on CaA zeolite. Owing to the high contents of naphthenic and isoparaffinic hydrocarbons and the low content of aromatic hydrocarbons in the distillate part, this crude can be used to produce high-quality fuels and oils by the use of the dewaxing processes.

  2. Arsenic removal from water using lignocellulose adsorption medium (LAM).

    PubMed

    Kim, Juyoung; Mann, Justin D; Spencer, Jesse G

    2006-01-01

    Arsenate in water is readily adsorbed on lignocellulose adsorption medium (LAM) which is cotton-based and has been coated with iron(III) by soaking cotton pellets in ferric chloride solution. Capacities achieved with LAM average 32.8 mg As/g of medium at influent arsenic concentrations ranging from 20-30 mg As/L. Adsorption follows (R2 = 0.993) a Freundlich isotherm, (x/M) = 22.845 Ce0.25, where (x/M) is the ratio of milligrams of contaminant adsorbed per gram of adsorbent and Ce is the equilibrium concentration. As is often the case with adsorption from solution, the fit using a Langmuir isotherm was not as good (R2 = 0.8786). The adsorbent when saturated can be regenerated by treatment with dilute sodium hydroxide. After five regenerations, the capacity dropped by 11.5%. Arsenate washed off the adsorbent after regeneration, as well as that left on the medium, may be concentrated and disposed of properly or perhaps recycled. Consideration of costs shows that one ton of iron(III)-treated adsorbent can be used to remove arsenate at toxic levels from drinking water at a cost of about 3.20/ton US dollars plus the cost of media without regeneration. PMID:16835109

  3. Anatomical investigation of flower of Butea monosperma Lam.

    PubMed Central

    Muthuswamy, Ragunathan; Senthamarai, R.

    2014-01-01

    Background: Butea frondosa Roxb. and Koen. syn. Butea monosperma Lam. (Leguminosae or Fabaceae) is a tree grows up to the height of 8 m at the age 50 years. Its flowers are being used in traditional medicine for the treatment of ulcer, inflammation, hepatic disorder, and eye diseases. Aims: The present study was aimed at establishing the microscopic characteristics of flower B. monosperma Lam. Materials and Methods: Histological evaluation of flowers was done using standard procedures. Images of microscopic characters were taken at different magnifications using Nikon Labphoto 2 microscopic Unit. Perkin Elmer 5000 an atomic absorption spectrophotometer was employed for elemental analysis. Results: In the study, microscopic characters of floral parts were investigated in transverse section and the flower powder. The current study reveals the presence of pollen grains, ovary (OV), and trichomes in their flower powder. Different cell components were studied, and their sizes were measured. Elemental analysis showed the presence of Zn 52.2 ?g/g and Cu 36.3 ?g/g were major contents, whereas Cr, Mn, and Pd were minor contents in dried flower powder. Conclusion: The current study paves the way to provide standard information related to the presence of essential elements in the flower. Microscopic characters of the flower and its quantitative measurement of cell components will help to identify the plant and also help to improvise the existing monograph of B. monosperma in the Ayurvedic pharmacopoeia. PMID:25861140

  4. Berberis libanotica extract targets NF-?B/COX-2, PI3K/Akt and mitochondrial/caspase signalling to induce human erythroleukemia cell apoptosis.

    PubMed

    Diab, Saada; Fidanzi, Chloe; Léger, David Y; Ghezali, Lamia; Millot, Marion; Martin, Frédérique; Azar, Rania; Esseily, Fadi; Saab, Antoine; Sol, Vincent; Diab-Assaf, Mona; Liagre, Bertrand

    2015-07-01

    The aim of this study was to describe and understand the relationship between cyclooxygenase-2 (COX-2) expression and apoptosis rate in erythroleukemia cells after apoptosis induction by Berberis libanotica (Bl) extract. To achieve this goal we used erythroleukemia cell lines expressing COX?2 (HEL cell line) or not (K562 cell line). Moreover, we made use of COX?2 cDNA to overexpress COX?2 in K562 cells. In light of the reported chemopreventive and chemosensitive effects of natural products on various tumor cells and animal models, we postulated that our Bl extract may mediate their effects through apoptosis induction with suppression of cell survival pathways. Our study is the first report on the specific examination of intrinsic apoptosis and Akt/NF-?B/COX?2 pathways in human erythroleukemia cells upon Bl extract exposure. Even if Bl extract induced apoptosis of three human erythroleukemia cell lines, a dominant effect of Bl extract treatment on K562 cells was observed resulting in activation of the late markers of apoptosis with caspase-3 activation, PARP cleavage and DNA fragmentation. Whereas, we showed that Bl extract reduced significantly expression of COX?2 by a dose-dependent manner in HEL and K562 (COX?2+) cells. Furthermore, in regard to our results, it is clear that the simultaneous inhibition of Akt and NF-?B signalling can significantly contribute to the anticancer effects of Bl extract in human erythroleukemia cells. We observed that the Bl extract is clearly more active than the berberine alone on the induction of DNA fragmentation in human erythro-leukemia cells. PMID:25997834

  5. Analysis of hydroxycinnamic acids derivatives in calafate (Berberis microphylla G. Forst) berries by liquid chromatography with photodiode array and mass spectrometry detection.

    PubMed

    Ruiz, Antonieta; Mardones, Claudia; Vergara, Carola; Hermosín-Gutiérrez, Isidro; von Baer, Dietrich; Hinrichsen, Patricio; Rodriguez, Roberto; Arribillaga, Diego; Dominguez, Erwin

    2013-03-15

    Calafate (Berberis microphylla G. Forst) is a Patagonian barberry very rich in anthocyanins and one of the fruits with the highest levels of these polyphenols. Other phenolic compounds have also been described in calafate berries. However, to the best of our knowledge there is no available information on hydroxycinnamic acid derivatives. The complexity of hydroxycinnamic acids determination in calafate berries, due to their structure similarities and the interference of high anthocyanin concentration is addressed by means of solid liquid extraction, followed by solid phase extraction clean-up on MCX columns and HPLC-DAD-ESI-MS/MS. The optimized extraction, clean-up and HPLC separation method allowed the assignation of identity and quantification of 20 hydroxycinnamic acids from calafate fruits. 5-Caffeoylquinic acid was the main compound found in all the studied samples. Other 13 hydroxycinnamoyl quinic acids and 6 caffeic acid esters with aldaric acid derivatives assigned as glucaric acid were also identified. Moreover, the glucaric-based hydroxycinnamic acid derivatives accounted for almost the half of total content of this kind of phenolic compounds. The total concentration of hydroxycinnamic acids derivatives ranged between 0.32±0.00 ?mol/g and 8.28±0.01 ?mol/g. Effect of ripening and geographical location on hydroxycinnamic acid profiles and concentrations are also evaluated. The methodology allows the determination of hydroxycinnamic acids from calafate despite of the high anthocyanin concentrations, showing a much higher concentration of these acids than other widely consumed berries. Thus suggesting that calafate could be considered a very interesting fruit from the point of view of their nutraceutical composition. However, geographical location and ripening have incidence in levels of studied compounds. PMID:23398997

  6. Determination of color, pigment, and phenolic stability in yogurt systems colored with nonacylated anthocyanins from Berberis boliviana L. as compared to other natural/synthetic colorants.

    PubMed

    Wallace, T C; Giusti, M M

    2008-05-01

    Anthocyanins are of interest to the food industry because of their antioxidant power, attractive color, and stability in high acid foods. Powder from the Peruvian berry Berberis boliviana Lechler, rich in nonacylated anthocyanins (7% to 8% dry weight), was incorporated into yogurt samples containing 3 different fat levels. Color (CIE L, a, b, chroma, and hue angle), pigment (monomeric anthocyanin and polymeric color), and total phenolics were monitored over 8 wk of storage and compared to yogurt treatments containing purple carrot acylated anthocyanins, red beet betalaines, or FD&C Red nr 40. Anthocyanin profiles were analyzed by HPLC coupled to photodiode array and mass detectors. Color of yogurt containing B. boliviana anthocyanins at 20 mg cyanidin-3-glucoside (cy-3-glu) equivalents/100 g yogurt (L*= 65, chroma = 14, and hue angle = 335 degrees ) was similar to commercial blueberry yogurt (L*= 65, chroma = 10.5, and hue angle = 341 degrees ). High color, pigment, and phenolic stability were observed in yogurts colored with B. boliviana, independent of the fat matrix. Acylated anthocyanins from purple carrot extracts exhibited increased stability with higher fat content. Anthocyanin degradation followed 1st-order kinetics. Pigment half-lives were 125 and 104 d for nonacylated anthocyanins at 10 and 20 mg cy-3-glu equivalents/100 g yogurt and 550.2, 232.6, and 128.9 d for acylated anthocyanins at 20 mg of cy-3-glu equivalents/100 g of 4%, 2%, and 0% fat yogurt. Addition of B. boliviana whole berry powder to yogurt matrices produced an attractive, stable anthocyanin-rich product, eliminating the need for industrial colorant extraction. PMID:18460117

  7. Analysis of Lymphangioleiomyomatosis (LAM) patent tomograms: Stereology and modeling

    SciTech Connect

    Faber, V.; Wing, G.M.; Winter, L.; Zahrt, J.D. [Los Alamos National Lab., NM (United States). Computing, Information, and Communications Div.

    1995-02-01

    Lymphangioleiomyomatosis (LAM) is an insidious disease of young women which attacks the lungs and if untreated results in death. Treatment is radical. Currently a woman diagnosed as having LAM (usually by computed tomography (CT) of the lung) is run through an exhausting set of tests and exercises to determine the extent of the disease. Image processing techniques have been able to quantify the tomograms by providing a histogram of the number of cyst intercepts of a given area. The images are digital, so the data are discrete, and the areas are in terms of square pixels. It is hoped that by analyzing the data and by recovering the probability density of the cysts themselves one can provide a set of parameters that will aid in the early diagnosis of the disease and that will correlate well with the physiology. The desire to invert the data, that is to determine the probability density of the cysts from the probability density of the circular intersections of the cysts with the tomographic plane, led to a re-examination of the Wicksell equation. In Section 2, the authors transform Wicksell`s equation in diameter (a generalized Abel equation) into an equation in areas (the original Abel equation). In Section 3 they present the adjoint equation in several equivalent forms. Analytic solutions of the adjoint equation necessary for later use are developed in Section 4. Outside of the framework of integral equations they have done some modeling of the data. They find that, to a very high degree of reliability, the data follows a simple power law. The distribution of spheres likewise follows a simple power law. These considerations are given in Section 5. Then in Section 6 they explore the exponential model as an alternative. Section 7 solves the Wicksell equation and uses the Golberg device, via the adjoint, to solve a related problem. They finally get to the number of cysts and their average size in Section 8. Their conclusions are in Section 9.

  8. 75 FR 76952 - Grant of Authority for Subzone Status; Lam Research Corporation (Wafer Fabrication Equipment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-10

    ...Subzone Status; Lam Research Corporation (Wafer Fabrication Equipment) Fremont, Newark...establish a special-purpose subzone at the wafer fabrication equipment manufacturing and...to the manufacturing and distribution of wafer fabrication equipment at the...

  9. No categorial support for radical ontic structural realism Vincent Lam and Christian Wuthrich

    E-print Network

    Wüthrich, Christian

    No categorial support for radical ontic structural realism Vincent Lam and Christian W¨uthrich Abstract Radical ontic structural realism (ROSR) asserts an ontological commitment to `free to ROSR. Keywords: radical ontic structural realism, category-theoretic structure, topological quantum

  10. Analgesic and anti-inflammatory effects of Cassia siamea Lam. stem bark extracts

    Microsoft Academic Search

    G. F. Nsonde Ntandou; J. T. Banzouzi; B. Mbatchi; R. D. G. Elion-Itou; A. W. Etou-Ossibi; S. Ramos; F. Benoit-Vical; A. A. Abena; J. M. Ouamba

    2010-01-01

    Aim of the studyThe present study was carried out to investigate analgesic and anti-inflammatory activities of Cassia siamea Lam stem bark extracts. We have also determined the cytotoxicity of each extract.

  11. Jackfruit ( Artocarpus heterophyllus Lam.) Diversity in Bangladesh: Land Use and Artificial Selection 1

    Microsoft Academic Search

    Ruby Khan; Nyree Zerega; Salma Hossain; M. I. Zuberi

    2010-01-01

    Jackfruit (\\u000a Artocarpus heterophyllus\\u000a Lam.) Diversity in Bangladesh: Land Use and Artificial Selection. Bangladesh is often recognized as a secondary center of diversity for jackfruit (Artocarpus heterophyllus Lam.) and is expected to harbor important genetic diversity of this underutilized tree crop. Unfortunately, genetic erosion\\u000a is occurring before this rich local resource has been documented and utilized for potential crop improvement.

  12. Laboratoire Langues, Littratures, Linguistique des Universits d'Angers et du Maine (3L.AM) -

    E-print Network

    Di Girolami, Cristina

    Laboratoire Langues, Littératures, Linguistique des Universités d'Angers et du Maine (3L.AM) - EA n Laboratoire Langues, Littératures, Linguistique des universités d'Angers et du Maine (Labo 3L.AM), �quipe d au Mans et 20 à Angers) et accueille 40 doctorants. #12;Au Mans, le laboratoire est dirigé par Franck

  13. Why Steiner-tree type algorithms work for community detection Mung Chiang Henry Lam Zhenming Liu Vincent Poor

    E-print Network

    Lam, Henry

    Why Steiner-tree type algorithms work for community detection Mung Chiang Henry Lam Zhenming Liu Vincent Poor Princeton University Boston University Princeton University Princeton University Abstract We

  14. New phytoconstituents from the aerial parts of Fumaria parviflora Lam.

    PubMed

    Jameel, Mohammad; Ali, Abuzer; Ali, Mohammed

    2014-04-01

    Fumaria parviflora Lam. (Fumariaceae) is an annual herb found throughout the world. Traditionally it has great significance in various disorders. In folk medicine of Turkey it is used against hepato-biliary dysfunction and imported from Iran. In Charaka and Sushruta, it is recommended for treatment of fevers, blood disorders, chronic skin diseases, urinary diseases and cough. The compounds were isolated from methanolic extract of the plants by column chromatography using silica gel (60-120 mesh) as stationary phase and structure of the isolated compounds have been established on the basis of spectral data analysis and chemical reactions. Phytochemical investigation of its aerial parts led to the isolation of five new compounds characterized as (5?H,11?H)-8-oxo-homoiridolide (1), n-docosanyl-2-O-?-D-glucopyranosyl salicylate (2), 2-methyl-6-hydroxymethylenedodecan-10-oyl-12, 15-olide14-O-?-D-xylopyranoside (3), 4-oxo-stigmast-5-en-3?-ol-D-glucopyranoside (4) and salicylic acid-O-?-D-xylopyranoside (5) along with the known compounds ?-D-glucopyranosyl hexadecanoate (6) and ?-D-glucopyranosyl- (2 ? 1')-?-D-glucopyranoside (7). The isolated compounds are useful as they will provide essential data and information for the further researchers and development of effective analytical marker for identity, purity and quality control of this traditional plant in future. PMID:24959414

  15. Laser Ablation Mass Spectrometer (LAMS) as a Standoff Analyzer in Space Missions for Airless Bodies

    NASA Technical Reports Server (NTRS)

    Li, X.; Brinckerhoff, W. B.; Managadze, G. G.; Pugel, D. E.; Corrigan, C. M.; Doty, J. H.

    2012-01-01

    A laser ablation mass spectrometer (LAMS) based on a time-of-flight (TOF) analyzer with adjustable drift length is proposed as a standoff elemental composition sensor for space missions to airless bodies. It is found that the use of a retarding potential analyzer in combination with a two-stage reflectron enables LAMS to be operated at variable drift length. For field-free drift lengths between 33 cm to 100 cm, at least unit mass resolution can be maintained solely by adjustment of internal voltages, and without resorting to drastic reductions in sensitivity. Therefore, LAMS should be able to be mounted on a robotic arm and analyze samples at standoff distances of up to several tens of cm, permitting high operational flexibility and wide area coverage of heterogeneous regolith on airless bodies.

  16. Evaluating educational media using traditional folk songs ('lam') in Laos: a health message combined with oral tradition.

    PubMed

    Yoshida, Itsuko; Kobayashi, Toshio; Sapkota, Sabitri; Akkhavong, Kongsap

    2012-03-01

    In the Lao People's Democratic Republic (Laos), health education is clearly a core aspect of the health service and is vital in improving people's lives through good health. However, there are many obstacles to conducting effective health education. The development of effective educational media is one solution to these problems. In Laos, traditional folk songs (lam) are preserved as part of the local communication media, and recently this communication medium has been used for health education. The aim of this study is to evaluate the effectiveness of educational media using lam. For this purpose, we conducted focus group discussions with 48 participants. The reactions of the participants towards a lam, developed for preventing HIV/AIDS, were analysed using the KJ (Kawakita Jiro) method. The analysis showed there were eight areas of concern: (1) interest in a lam talking about HIV/AIDS; (2) knowledge and perception related to HIV infection routes; (3) expressing a willingness for preventing HIV/AIDS; (4) togetherness with people living with HIV/AIDS; (5) HIV/AIDS education for children; (6) improving educational methods; (7) characteristics and effectiveness of the lam and (8) song preferences. The reactions of the participants, such as gaining knowledge and expressing individual attitudes and community actions for preventing HIV/AIDS, were promoted by the characteristics and effectiveness of the lam such as oral tradition, artistry and cultural values. In particular, the oral tradition represented by lam is useful for the Lao people in memorizing and communicating information. PMID:22100506

  17. Phytochemistry, nutritional and pharmacological properties of Artocarpus heterophyllus Lam (jackfruit): A review

    Microsoft Academic Search

    Manjeshwar Shrinath Baliga; Arnadi Ramachandrayya Shivashankara; Raghavendra Haniadka; Jerome Dsouza; Harshith P. Bhat

    2011-01-01

    Artocarpus heterophyllus Lam, commonly known as the jackfruit tree and belonging to the family Moraceae, is an exotic tree originally native to the Western Ghats of India. The fruits are of dietary use and are an important source of carbohydrate, protein, fat, minerals and vitamins. The heart wood is a very durable timber and is used in the preparation of

  18. Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers

    Microsoft Academic Search

    S. Shyamalamma; S. B. C. Chandra; M. Hegde; P. Naryanswamy

    2008-01-01

    Artocarpus heterophyllus Lam., commonly called jackfruit, is a medium-sized evergreen tree that bears high yields of the largest known edible fruit. Yet, it has been little explored commercially due to wide variation in fruit quality. The genetic di- versity and genetic relatedness of 50 jackfruit accessions were stud- ied using amplified fragment length polymorphism markers. Of 16 primer pairs evaluated,

  19. Touch and Run with Near Field Communication (NFC) Ben Dodson Hristo Bojinov Monica S. Lam

    E-print Network

    Straight, Aaron

    Touch and Run with Near Field Communication (NFC) Ben Dodson Hristo Bojinov Monica S. Lam Computer explore new ways in which Near Field Com- munication (NFC) can be used on smart phones. NFC al- lows concerns. 1. INTRODUCTION NFC is a radio technology that supports transactions at dis- tances of a few

  20. Analyze Influenza Virus Sequences Using Binary Encoding HamChing Lam

    E-print Network

    Boley, Daniel

    Analyze Influenza Virus Sequences Using Binary Encoding Approach HamChing Lam Dept. of Computer 55455, USA boley@cs.umn.edu ABSTRACT Capturing mutation patterns of each individual influenza virus distance. We first convert the influenza genetic sequence to a binary string and then ap- ply Principal

  1. Catching Old Influenza Virus with A New Markov Model HamChing Lam

    E-print Network

    Boley, Daniel

    Catching Old Influenza Virus with A New Markov Model HamChing Lam Dept. of Computer Science on the Hemag- glutinin (HA) gene, a major surface antigen of the avian influenza virus. Using this model we Influenza virus, Poisson process, Markov Model 1. INTRODUCTION For the past century researchers have been

  2. Visible-light-mediated chan-lam coupling reactions of aryl boronic acids and aniline derivatives.

    PubMed

    Yoo, Woo-Jin; Tsukamoto, Tatsuhiro; Kobayashi, Sh?

    2015-05-26

    The copper(II)-catalyzed aerobic oxidative coupling reaction between aryl boronic acids and aniline derivatives was found to be improved significantly under visible-light-mediated photoredox catalysis. The substrate scope of this oxidative Chan-Lam reaction was thus expanded to include electron-deficient aryl boronic acids as viable starting materials. PMID:25873290

  3. Chapter 7.13 Lam8 Poly-Si TCP Etcher

    E-print Network

    Healy, Kevin Edward

    single crystal silicon, polycrystalline silicon, silicon carbide, silicon germanium, and germanium films, cassette-to-cassette, TCP etcher for Poly-Silicon etching. This tool is currently dedicated to etching be programmed to end the etch process at a specified condition. Lam8 uses Channel A (405 nm for poly-silicon

  4. Phytochemistry, traditional uses and pharmacology of Eugenia jambolana Lam. (black plum): A review

    Microsoft Academic Search

    Manjeshwar Shrinath Baliga; Harshith P. Bhat; Bantwal Raghavendra Vittaldas Baliga; Rajesh Wilson; Princy Louis Palatty

    2011-01-01

    Eugenia jambolana Lam. (syn. Syzigium cumini (L.) SKEELS; S. jambolana DC; Family: Myrtaceae), commonly known as black plum or Jamun is a plant native to India. Annually the trees produce oblong or ellipsoid fruits (berries). They are green when raw and purplish black when fully ripe. The ripe fruits are sweetish sour to taste and are used to prepare health

  5. ROME: Routing On Metropolitan-scale Ethernet Chen Qian and Simon S. Lam

    E-print Network

    Hayes, Jane E.

    ROME: Routing On Metropolitan-scale Ethernet Chen Qian and Simon S. Lam Department of Computer the architecture and protocols of ROME, a layer-2 network designed to be backwards compatible with Eth- ernet and scalable to tens of thousands of switches and millions of end hosts. ROME is based upon a recently

  6. Heterogeneous Parallel Programming in Jade Martin C. Rinard, Daniel J. Scales and Monica S. Lam

    E-print Network

    Rinard, Martin

    Heterogeneous Parallel Programming in Jade Martin C. Rinard, Daniel J. Scales and Monica S. Lam Computer Systems Laboratory Stanford University, CA 94305 Abstract This paper presents Jade, a high-level parallel pro- gramming language for managing coarse-grain concur- rency. Jade simplifies programming

  7. Semantic Foundations of Jade Martin C. Rinard and Monica S. Lam

    E-print Network

    Rinard, Martin

    Semantic Foundations of Jade Martin C. Rinard and Monica S. Lam Computer Systems Laboratory Stanford University, CA 94305 Abstract Jade is a language designed to support coarse­grain parallelism on both shared and distributed address­ space machines. Jade is data­oriented: a Jade pro­ grammer simply

  8. Heterogeneous Parallel Programming in Jade Martin C. Rinard, Daniel J. Scales and Monica S. Lam

    E-print Network

    Rinard, Martin

    Heterogeneous Parallel Programming in Jade Martin C. Rinard, Daniel J. Scales and Monica S. Lam Computer Systems Laboratory Stanford University, CA 94305 Abstract This paper presents Jade, a high­level parallel pro­ gramming language for managing coarse­grain concur­ rency. Jade simplifies programming

  9. Coarse-Grain Parallel Programming in Jade Monica S. Lam and Martin C. Rinard

    E-print Network

    Rinard, Martin

    Coarse-Grain Parallel Programming in Jade Monica S. Lam and Martin C. Rinard Computer Systems Laboratory Stanford University, CA 94305 Abstract This paper presents Jade, a language which allows a pro the program's data dependence constraints. Using Jade can significantly re- duce the time and effort required

  10. Variação sazonal em antioxidantes em folhas de plantas jovens de Caesalpinia echinata Lam. (pau-brasil)

    Microsoft Academic Search

    Patricia Bulbovas; Mirian C. S. Rinaldi; Welington B. C. Delitti; Marisa Domingos

    2005-01-01

    Seasonal variation in antioxidants in leaves of young plants of Caesalpinia echinata Lam. (brazilwood)). The urban environmental factors generate conditions of oxidative stress in plants. Cellular antioxidants, such as ascorbic acid (AA), thiols (Ti), peroxidases (POD) and superoxide dismutase (SOD) are induced to different levels depending on their capacity to tolerate stressing conditions. In the first part of this study

  11. How does nitrogen availability alter rhizodeposition in Lolium multiflorum Lam. during vegetative growth?

    Microsoft Academic Search

    Frédéric Henry; Christophe Nguyen; Eric Paterson; Allan Sim; Christophe Robin

    2005-01-01

    The objective of this work was to determine if the impact of nitrogen (N) on the release of organic carbon (C) into the soil\\u000a by roots (rhizodeposition) correlated with the effect of this nutrient on some variables of plant growth. Lolium multiflorum Lam. was grown at two levels of N supply, either in sterile sand percolated with nutrient solution or

  12. QTL analysis of lodging resistance and related traits in Italian ryegrass ( Lolium multiflorum Lam.)

    Microsoft Academic Search

    Maiko Inoue; Zhensheng Gao; Hongwei Cai

    2004-01-01

    Italian ryegrass ( Lolium multiflorum Lam.) is the most widely cultivated annual forage grass in Japan. Lodging damage reduces both harvested yield and forage quality. To identify the chromosomal regions controlling lodging resistance in Italian ryegrass, we analyzed seven quantitative characters—heading date, plant height, culm weight, culm diameter, culm strength, tiller number, and culm pushing resistance—and evaluated lodging scores in

  13. Predicting weed migration from soil and climate maps. [Centaurea maculosa Lam

    Microsoft Academic Search

    T. K. Chicoine; P. K. Fay; G. A. Nielsen

    1985-01-01

    Soil characteristics, elevation, annual precipitation, potential evapotranspiration, length of frost-free season, and mean maximum July temperature were estimated for 116 established infestations of spotted knapweed (Centaurea maculosa Lam. number³ CENMA) in Montana using basic land resource maps. Areas potentially vulnerable to invasion by the plant were delineated on the basis of representative edaphic and climatic characteristics. No single environmental variable

  14. Influence of dehulling pretreatment on physicochemical properties of Gleditsia sinensis Lam. gum

    Microsoft Academic Search

    Hong-lei Jian; Carrasco Cristhian; Wei-ming Zhang; Jian-xin Jiang

    2011-01-01

    The seeds of Gleditsia sinensis Lam., widespread in China, are an important source of galactomannans. G. sinensis gum was extracted from whole seeds using baking, boiling water and acid dehulling pretreatment, respectively. The physicochemical properties of the three isolated G. sinensis gums were studied to evaluate three pretreatment methods. The results illustrated that the properties of G. sinensis gum were significantly

  15. Reflection Analysis for Java Benjamin Livshits, John Whaley, and Monica S. Lam

    E-print Network

    Livshits, Ben

    Reflection Analysis for Java Benjamin Livshits, John Whaley, and Monica S. Lam Computer Science. Reflection has always been a thorn in the side of Java static analysis tools. Without a full treatment of reflection, static analysis tools are both incomplete because some parts of the program may not be in- cluded

  16. Antifungal activity of crude extracts and essential oil of Moringa oleifera Lam

    Microsoft Academic Search

    Ping-Hsien Chuang; Chi-Wei Lee; Jia-Ying Chou; M. Murugan; Bor-Jinn Shieh; Hueih-Min Chen

    2007-01-01

    Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medicines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum, and Microsporum canis. GC–MS analysis of the chemical composition of the essential oil from leaves showed a total of 44 compounds. Isolated

  17. New ?-glucosides of caffeoyl quinic acid from the leaves of Moringa oleifera Lam.

    PubMed

    Kashiwada, Yoshiki; Ahmed, Fakhruddin Ali; Kurimoto, Shin-ichiro; Kim, Sang-Yong; Shibata, Hirofumi; Fujioka, Toshihiro; Takaishi, Yoshihisa

    2012-01-01

    Two new caffeoyl quinic acid ?-glucosides, together with three known caffeoyl quinic acids and five known flavonoid glucosides, were isolated from the leaves of Moringa oleifera Lam. The structures of the new compounds were elucidated as 4-O-(4'-O-?-D-glucopyranosyl)-caffeoyl quinic acid (1) and 4-O-(3'-O-?-D-glucopyranosyl)-caffeoyl quinic acid (2) by spectroscopic analyses. PMID:21748486

  18. mTOR Activation, Lymphangiogenesis, and Estrogen-Mediated Cell Survival: The “Perfect Storm” of Pro-Metastatic Factors in LAM Pathogenesis

    PubMed Central

    Yu, Jane

    2010-01-01

    Abstract Research interest in lymphangioleiomyomatosis (LAM) has grown dramatically in the past decade, particularly among cancer biologists. There are at least two reasons for this: first, the discovery in the year 2000 that LAM cells carry TSC2 gene mutations, linking LAM with cellular pathways including the PI3K/Akt/mTOR axis, and allowing the Tuberous Sclerosis Complex (TSC)-regulated pathways that are believed to underlie LAM pathogenesis to be studied in cells, yeast, Drosophila, and mice. A second reason for the rising interest in LAM is the discovery that LAM cells can travel to the lung, including repopulating a donor lung after lung transplantation, despite the fact that LAM cells are histologically benign. This “benign metastasis” underpinning suggests that elucidating LAM pathogenesis will unlock a set of fundamental mechanisms that underlie metastatic potential in the context of a cell that has not yet undergone malignant transformation. Here, we will outline the data supporting the metastatic model of LAM, consider the biochemical and cellular mechanisms that may enable LAM cells to metastasize, including both cell autonomous and non-cell autonomous factors, and highlight a mouse model in which estrogen promotes the metastasis and survival of TSC2-deficient cells in a MEK-dependent manner. We propose a multistep model of LAM cell metastasis that highlights multiple opportunities for therapeutic intervention. Taken together, the metastatic behavior of LAM cells and the involvement of tumor-related signaling pathways lead to optimism that cancer-related paradigms for diagnosis, staging, and therapy will lead to therapeutic breakthroughs for women living with LAM. PMID:20235886

  19. Biosynthesis and Virulent Behavior of Lipids Produced by Mycobacterium tuberculosis: LAM and Cord Factor: An Overview.

    PubMed

    Rajni; Rao, Nisha; Meena, Laxman S

    2011-01-01

    Mycobacterium tuberculosis is the causative agent of tuberculosis disease, which has developed a myriad of exceptional features contributing to its survival within the hostile environment of host cell. Unique cell wall structure with high lipid content plays an imperative role in the pathogenicity of mycobacteria. Cell wall components of MTB such as lipoarabinomannan and Trehalose dimycolate (cord factor) are virulent in nature apart from its virulence genes. Virulent effect of these factors on host cells reduces host cell immunity. LAM has been known to inhibit phagosome maturation by inhibiting the Ca(2+)/calmodulin phosphatidyl inositol-3-kinase hvps34 pathways. Moreover, TDM (Trehalose dimycolate) also inhibits fusion between phospholipid vesicles and migration of polymorphonuclear neutrophils. The objective of this paper is to understand the virulence of LAM and cord factor on host cell which might be helpful to design an effective drug against tuberculosis. PMID:21350659

  20. Bionic lightweight design by laser additive manufacturing (LAM) for aircraft industry

    NASA Astrophysics Data System (ADS)

    Emmelmann, Claus; Petersen, Maren; Kranz, Jannis; Wycisk, Eric

    2011-05-01

    Today many challenges lie ahead of the aircraft industry. The increasing competition and shortage of resources raise a challenge for future manufacturing technologies and lightweight design. A possibility to cope with these circumstances is the manufacturing technology of Laser Additive Manufacturing (LAM). However there are still challenges to cope with due to the processes novelty, such as the development of further materials, especially lightweight alloys, and new design approaches. Therefore innovative approaches for material development and lightweight design were created in order to fully exploit the processes potentials. The material development process is based on an analytical calculation of temperature distribution versus effective process factors in order to identify acceptable operating conditions for the LAM process. A novel approach to extreme lightweight design was realized by incorporating structural optimization tools and bionic structures into one design process. By consequently following these design principles, designers can achieve lightweight savings in designing new aircraft structure and push lightweight design to new limits.

  1. Assessing morphological and isozyme variation of jackfruit ( Artocarpus heterophyllus Lam.) in Bangladesh

    Microsoft Academic Search

    A. K. Azad; J. G. Jones; N. Haq

    2007-01-01

    A farmers’ participatory survey was carried out in five different agroecosystems of Bangladesh to obtain information on the\\u000a genetic diversity, consumers preferences for fruit quality and to assess the data to select potentially superior genotypes\\u000a of jackfruit (Artocarpus heterophyllus Lam.). A wide variation in morpho-agronomic characters was identified from farmers’ information and also from laboratory\\u000a analyses. Multivariate analysis of the

  2. Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)

    Microsoft Academic Search

    M. A. Rahman; Jennet Blake

    1988-01-01

    Successful vegetative propagation of seedling jackfruit (Artocarpus heterophyllus Lam.) has been achieved by in vitro methods. Proliferation from nodal explants was greater than from shoot tips. Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M. Shoot proliferation was optimal at 30°C with a 12 h

  3. Evaluation of Antioxidant Capacity and Phenol Content in Jackfruit ( Artocarpus heterophyllus Lam.) Fruit Pulp

    Microsoft Academic Search

    Umesh B. Jagtap; Shrimant N. Panaskar; V. A. Bapat

    2010-01-01

    The antioxidant capacity of jackfruit (Artocarpus heterophyllus Lam. Fam. Moracae) fruit pulp (JFP) obtained from Western Ghats India was determined by evaluating the scavenging activity\\u000a using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing power assays and N, N-dimethyl-p-phenylendiamine (DMPD) radical cation decolorization assay. JFP was analyzed for total phenolic content (TPC) and total flavonoids\\u000a content (TFC). The ethanol and water are the best

  4. Photoelectrochemical solar cell using extract of Eugenia jambolana Lam as a natural sensitizer

    Microsoft Academic Search

    Christian G. Garcia; André S. Polo; Neyde Y. Murakami Iha

    2003-01-01

    The extract of Jambolão (java plum), Eugenia jambolanaLam, was used as a natural sensitizer of a wide band-gap semiconductor (TiO2) in photoelectrochemical solar cells. The natural dye, adsorbed onto the semiconductor surface, absorbs visible light and promotes electron transfer across the dye\\/semiconductor interface. Photogenerated current and voltage as high as 2.3 mA and 711 mV, respectively, were obtained and effective

  5. Chemical Composition of the Essential Oil of Artemisia herba-alba Asso ssp. valentina (Lam.) Marcl

    Microsoft Academic Search

    Sofía Salido; Joaquín Altarejos; Manuel Nogueras; Adolfo Sánchez

    2001-01-01

    The composition of the oil, steam-distilled from aerial parts of Artemisia herha-alba Asso ssp. valentina (Lam.) Marcl. (Asteraceae) collected from the south of Spain, has been analyzed by GC\\/MS. Among the 65 constituents investigated (representing 93.6 % of the oil composition), 61 were identified (90.3% of the oil composition). The major constituents detected were the sesquiterpene davanone (18.1%) and monoterpenes

  6. Antibacterial activity of methanol extract of Ruta chalapensis (L), Quercus infectoria (Oliver) and Canthium parviflorum (Lam)

    PubMed Central

    Priya, P. Sathiya; Sasikumar, J.M.; Gowsigan, G.

    2009-01-01

    The present study aimed at evaluating the antibacterial activity of methanol extract of Ruta chalapensis, L., (Rutaceae), Quercus infectoria Oliver., (Fagaceae) and Canthium parviflorum Lam., (Rubiaceae) against Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, Klebsiella oxytocoa, Klebsiella pneumoniae and Proteus mirabilis. The experiment was carried out using disc diffusion method. The results revealed that the methanol extract of aerial parts of Ruta chalepensis (L) presented the highest zone of inhibition against tested pathogens. Other plants showed significant zone of inhibition. PMID:22557348

  7. Antibacterial activity of methanol extract of Ruta chalapensis (L), Quercus infectoria (Oliver) and Canthium parviflorum (Lam).

    PubMed

    Priya, P Sathiya; Sasikumar, J M; Gowsigan, G

    2009-10-01

    The present study aimed at evaluating the antibacterial activity of methanol extract of Ruta chalapensis, L., (Rutaceae), Quercus infectoria Oliver., (Fagaceae) and Canthium parviflorum Lam., (Rubiaceae) against Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, Klebsiella oxytocoa, Klebsiella pneumoniae and Proteus mirabilis. The experiment was carried out using disc diffusion method. The results revealed that the methanol extract of aerial parts of Ruta chalepensis (L) presented the highest zone of inhibition against tested pathogens. Other plants showed significant zone of inhibition. PMID:22557348

  8. Gene Expression Rhythms in the Mussel Mytilus galloprovincialis (Lam.) across an Annual Cycle

    Microsoft Academic Search

    Mohamed Banni; Alessandro Negri; Flavio Mignone; Hamadi Boussetta; Aldo Viarengo; Francesco Dondero; Cynthia Gibas

    2011-01-01

    Seasonal environmental changes may affect the physiology of Mytilus galloprovincialis (Lam.), an intertidal filter-feeder bivalve occurring commonly in Mediterranean and Atlantic coastal areas. We investigated seasonal variations in relative transcript abundance of the digestive gland and the mantle (gonads) of males and females. To identify gene expression trends – in terms of relative mRNA abundance- we used a medium-density cDNA

  9. Purification and characterization of a trypsin inhibitor from the seeds of Artocarpus heterophyllus Lam.

    PubMed

    Lyu, Junchen; Liu, Yuan; An, Tianchen; Liu, Yujun; Wang, Manchuriga; Song, Yanting; Zheng, Feifei; Wu, Dan; Zhang, Yingxia; Deng, Shiming

    2015-05-01

    A proteinaceous inhibitor against trypsin was isolated from the seeds of Artocarpus heterophyllus Lam. by successive ammonium sulfate precipitation, ion-exchange, and gel-filtration chromatography. The trypsin inhibitor, named as AHLTI (A. heterophyllus Lam. trypsin inhibitor), consisted of a single polypeptide chain with a molecular weight of 28.5 kDa, which was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel-filtration chromatography. The N-terminal sequence of AHLTI was DEPPSELDAS, which showed no similarity to other known trypsin inhibitor sequence. AHLTI completely inhibited bovine trypsin at a molar ratio of 1:2 (AHLTI:trypsin) analyzed by native polyacrylamide gel electrophoresis, inhibition activity assay, and gel-filtration chromatography. Moreover, kinetic enzymatic studies were carried out to understand the inhibition mechanism of AHLTI against trypsin. Results showed that AHLTI was a competitive inhibitor with an equilibrium dissociation constant (Ki) of 3.7 × 10(-8) M. However, AHLTI showed weak inhibitory activity toward chymotrypsin and elastase. AHLTI was stable over a broad range of pH 4-8 and temperature 20-80°C. The reduction agent, dithiothreitol, had no obvious effect on AHLTI. The trypsin inhibition assays of AHLTI toward digestive enzymes from insect pest guts in vitro demonstrated that AHLTI was effective against enzymes from Locusta migratoria manilensis (Meyen). These results suggested that AHLTI might be a novel trypsin inhibitor from A. heterophyllus Lam. belonging to Kunitz family, and play an important role in protecting from insect pest. PMID:25851516

  10. Effect of different growth stages of Ziziphora clinopodioides Lam. on its chemical composition

    PubMed Central

    Ding, Wenhuan; Yang, Tao; Liu, Feng; Tian, Shuge

    2014-01-01

    Aims: The aim of this study is to monitor the changes in the chemical composition of Ziziphora clinopodioides Lam. throughout nine different growth stages. Materials and Methods: Volatile components such as essential oils were analyzed using the gas chromatography (GC) and GC-mass spectrometry, and the contents of non-volatile components were determined by a visible spectrophotometer. Results: Hydro-distilled essential oil content ranged from a minimum of 1.1% (in the post-flowering stage) to a maximum of 1.8% (in the flowering stage). The essential oils included pulegone, which was the most abundant component (77.48-87.3%), p-menthanone (2.79-12.39%), trans-isopulegone (1.04-2.06%), d-limonene (0.51-3.03%) and eucarvone (1.5-4.48%). The contents of non-volatile components, such as that of total phenolics (TPC), total flavonoids (TFC), total triterpenoids content (TTC) and total free amino acids content (TFAAC) were measured using visible spectrophotometry. In the growing stage, TPC, TFC, TTC and TFAAC were 9.91-12.80 mg/g, 29.84-50.63 mg/g, 0.57-1.41 mg/g and 13.33-28.56 mg/g, respectively. Conclusion: These data can be used as a basis to determine the optimal harvest time of Z. clinopodioide Lam. PMID:24914287

  11. Antineoplastic potential of Bryophyllum pinnatum lam. on chemically induced hepatocarcinogenesis in rats

    PubMed Central

    Afzal, Muhammad; Kazmi, Imran; Anwar, Firoz

    2013-01-01

    Background: Bryophyllum pinnatum Lam. used in folk medicine in tropical Africa, tropical America, India, China and Australia contains a wide range of active compounds, well known for their haemostatic and wound-healing properties. Objective: The present study was designed to evaluate the effect of Bryophyllum pinnatum Lam. on N-diethylnitrosamine (DENA)-induced hepatic injury in rats. Material and Methods: The aerial part of B. pinnatum aqueous and ethanolic extract was prepared in doses of 250 mg/kg and 500 mg/kg. Hepatic injury was induced by DENA. Acute toxicity was also carried out. Result: Treatment with different doses of ethanolic extract of B. Pinnatum (250 mg/kg, p.o.) was not significantly able to treat the liver injury induced by DENA, but 500 mg/kg dose of ethanolic extract of B. Pinnatum protects the liver slightly. Treatment with different doses of aqueous extract of B. Pinnatum (250 and 500 mg/kg, p.o.) significantly (P*<0.05; P**<0.01 and P***<0.001) treated the liver injury induced by DENA. Conclusion: It may be inferred from the present study that the hepatoprotective activities of the aqueous extract of B. Pinnatum leaves in DENA-induced hepatotoxicity may involve its antioxidant or oxidative free radical scavenging activities by alleviating lipid peroxidation through scavenging of free radicals, or by enhancing the activity of antioxidants. PMID:24174817

  12. Silk: A Resilient Routing Fabric for Peer-to-Peer Networks Simon S. Lam and Huaiyu Liu

    E-print Network

    Lam, Simon S.

    Silk: A Resilient Routing Fabric for Peer-to-Peer Networks£ Simon S. Lam and Huaiyu Liu Dept named Silk, will be referred to as Silk pro- tocols. From simulation experiments in which the number of concurrent joins and failures was up to 50% of the initial net- work size, we found that, for à ¾, Silk

  13. Preface: Proceedings of the 13th Conference on Liquid and Amorphous Metals (LAM13) (Ekaterinburg, Russia, 8 14 July 2007)

    Microsoft Academic Search

    Pjotr Popel; Boris Gelchinskii; Valeriy Sidorov

    2008-01-01

    The most recent developments in the field of liquid and amorphous metals and alloys are regularly updated through two complementary international conferences: the liquid and amorphous metals conference (LAM) and the rapidly quenched materials (RQ) conference. The first series of conferences started as LM1 in 1966 at Brookhaven for the basic understanding of liquid metals. The subsequent LM conferences were

  14. Mining MultiDimensional Constrained Gradients in Data Cubes Guozhu Dong Jiawei Han Joyce Lam Jian Pei Ke Wang

    E-print Network

    Dong, Guozhu

    Mining Multi­Dimensional Constrained Gradients in Data Cubes #3; Guozhu Dong Jiawei Han Joyce Lam associated with big changes in measure in a data cube. Cells are considered similar if they are related and describe sectors of the business modeled by the data cube. The problem of mining changes of sophisticated

  15. Environmental Hydraulics and Sustainable Water Management Lee & Lam (eds) 2005 Taylor & Francis Group, London, ISBN 04 1536 546 5

    E-print Network

    Jiao, Jiu Jimmy

    Environmental Hydraulics and Sustainable Water Management ­ Lee & Lam (eds) © 2005 Taylor & Francis International Airport Fuli Wang Lincoln Ventures Ltd, Lincoln, Christchurch, New Zealand Jiu J. Jiao Department of Earth Sciences, University of Hong Kong, Hong Kong, China ABSTRACT: The Hong Kong InternationalAirport

  16. Towards a Formal Foundation of Web Security Devdatta Akhawe, Adam Barth, Peifung E. Lam, John Mitchell and Dawn Song

    E-print Network

    California at Irvine, University of

    Towards a Formal Foundation of Web Security Devdatta Akhawe, Adam Barth, Peifung E. Lam, John,abarth,dawnsong}@cs.berkeley.edu Stanford University {pflam,mitchell}@cs.stanford.edu Abstract--We propose a formal model of web security based on an abstraction of the web platform and use this model to analyze the security of several sample

  17. Hierarchical Concurrency in Jade Daniel J. Scales, Martin C. Rinard, Monica S. Lam, and Jennifer M. Anderson

    E-print Network

    Rinard, Martin

    Hierarchical Concurrency in Jade Daniel J. Scales, Martin C. Rinard, Monica S. Lam, and Jennifer M. Anderson Computer Systems Laboratory Stanford University, CA 94305 Abstract Jade is a data, or C++. A Jade programmer simply augments the program with assertions specify­ ing how the program

  18. The effects of floating mats of Azolla filiculoides Lam. and Lemna minuta Kunth on the growth of submerged macrophytes

    Microsoft Academic Search

    Rachel A. Janes; John W. Eaton; Keith Hardwick

    1996-01-01

    In laboratory experiments, the growth characteristics of the submerged species Elodea nuttallii (Planch.) St. John and Potamogeton crispus L. were assessed in the presence and absence of floating mats of Azolla filiculoides Lam. and Lemna minuta Kunth. Light penetration and the development of pH and dissolved oxygen differences were monitored. The growth of P. crispus was suppressed much more than

  19. Intergeneric somatic hybridization in Gramineae: somatic hybrid plants between tall fescue ( Festuca arundinacea Schreb.) and Italian ryegrass ( Lolium multiflorum Lam.)

    Microsoft Academic Search

    Tadashi Takamizo; German Spangenberg; Ken-ichi Suginobu; Ingo Potrykus

    1991-01-01

    Tall fescue (Festuca arundinacea Schreb.) protoplasts, inactivated by iodoacetamide, and non-morphogenic Italian ryegrass (Lolium multiflorum Lam.) protoplasts, both derived from suspension cultures, were electrofused and putative somatic hybrid plants were recovered. Two different genotypic fusion combinations were carried out and several green plants were regenerated in one of them. With respect to plant habitus, leaf and inflorescence morphology, the regenerants

  20. Contact-Printed Microelectromechanical Systems By Corinne E. Packard, Apoorva Murarka, Eric W. Lam, Martin A. Schmidt,

    E-print Network

    method to define 3D electromechanical structures. This technique relies on an organic molecular releaseContact-Printed Microelectromechanical Systems By Corinne E. Packard, Apoorva Murarka, Eric W. Lam of lithographic processing using an economically and dimensionally scalable, large-area microcontact printing

  1. Hybrid Security Architecture for Data Center Ho-Yu Lam, Song Zhao, Kang Xi, H. Jonathan Chao

    E-print Network

    Chao, Jonathan

    of data centers and cloud comput- ing services, security has become a challenge of increasing importanceHybrid Security Architecture for Data Center Networks Ho-Yu Lam, Song Zhao, Kang Xi, H. Jonathan, Brooklyn, NY Abstract--Security is critical to data centers, especially multi- tenant data centers

  2. ReTAX+: A Cooperative Taxonomy Revision Tool Sik Chun (Joey) Lam, Derek Sleeman, and Wamberto Vasconcelos

    E-print Network

    Vasconcelos, Wamberto

    1 ReTAX+: A Cooperative Taxonomy Revision Tool Sik Chun (Joey) Lam, Derek Sleeman, and Wamberto in an established taxonomy; it suggests a number of ways in which that can be achieved namely; modifying the new entity, the taxonomy, or both. Further, a set of refinement operators are used to guarantee

  3. Performance Comparison of LAM/MPI, MPICH, and MVICH on a Linux Cluster connected by a Gigabit Ethernet Network.

    E-print Network

    Farrell, Paul A.

    Ethernet Network. Hong Ong and Paul A. Farrell Department of Mathematics and Computer Science, Kent State the performance of LAM, MPICH, and MVICH on a Linux cluster connected by a Gigabit Ethernet network. Performance, cluster computing has become increasingly popular. Many research institutes, universities, and industrial

  4. Identifying and selecting for genetic diversity in Papua New Guinea sweetpotato Ipomoea batatas (L.) Lam. germplasm collected as botanical seed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Genetic analysis was conducted on 141 "Ipomoea batatas" L. (Lam.) Genotypes derived from botanical seed originally collected from 26 sites in 4 provinces in Papua New Guinea. Relatedness among accessions was estimated by analysis of the AFLP data using the Dice coefficient of similarity and UPGMA. ...

  5. BIRD: Binary Interpretation using Runtime Disassembly Susanta Nanda Wei Li Lap-Chung Lam Tzi-cker Chiueh

    E-print Network

    Sekar, R.

    number of computer system vulnerabilities are due to software bugs. Researchers have proposed variousBIRD: Binary Interpretation using Runtime Disassembly Susanta Nanda Wei Li Lap-Chung Lam Tzi a binary file with 100% accuracy and coverage on that platform. This pa- per presents the design

  6. Digital Gene Expression Analysis Based on Integrated De Novo Transcriptome Assembly of Sweet Potato [Ipomoea batatas (L.) Lam.

    Microsoft Academic Search

    Xiang Tao; Ying-Hong Gu; Hai-Yan Wang; Wen Zheng; Xiao Li; Chuan-Wu Zhao; Yi-Zheng Zhang

    2012-01-01

    BackgroundSweet potato (Ipomoea batatas L. [Lam.]) ranks among the top six most important food crops in the world. It is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. However, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. Hence, studies based

  7. The Aberdeen University Ontology Reuse Stack Edward Thomas, Derek Sleeman, Jeff Z. Pan, Quentin Reul and Joey Lam

    E-print Network

    Sleeman, Derek

    The Aberdeen University Ontology Reuse Stack Edward Thomas, Derek Sleeman, Jeff Z. Pan, Quentin Reul and Joey Lam Department of Computing Science University of Aberdeen Aberdeen, AB24 3FX Contact. ONTOSEARCH2 (Pan, Thomas, & Sleeman 2006) is a tool designed to allow end users of ontologies (knowledge

  8. MicroRNA-21 is Induced by Rapamycin in a Model of Tuberous Sclerosis (TSC) and Lymphangioleiomyomatosis (LAM)

    PubMed Central

    Neuman, Nicole A.; Myachina, Faina; Yu, Jane; Priolo, Carmen; Henske, Elizabeth P.

    2013-01-01

    Lymphangioleiomyomatosis (LAM), a multisystem disease of women, is manifest by the proliferation of smooth muscle-like cells in the lung resulting in cystic lung destruction. Women with LAM can also develop renal angiomyolipomas. LAM is caused by mutations in the tuberous sclerosis complex genes (TSC1 or TSC2), resulting in hyperactive mammalian Target of Rapamycin (mTOR) signaling. The mTOR inhibitor, Rapamycin, stabilizes lung function in LAM and decreases the volume of renal angiomyolipomas, but lung function declines and angiomyolipomas regrow when treatment is discontinued, suggesting that factors induced by mTORC1 inhibition may promote the survival of TSC2-deficient cells. Whether microRNA (miRNA, miR) signaling is involved in the response of LAM to mTORC1 inhibition is unknown. We identified Rapamycin-dependent miRNA in LAM patient angiomyolipoma-derived cells using two separate screens. First, we assayed 132 miRNA of known significance to tumor biology. Using a cut-off of >1.5-fold change, 48 microRNA were Rapamycin-induced, while 4 miRs were downregulated. In a second screen encompassing 946 miRNA, 18 miRs were upregulated by Rapamycin, while eight were downregulated. Dysregulation of miRs 29b, 21, 24, 221, 106a and 199a were common to both platforms and were classified as candidate “RapamiRs.” Validation by qRT-PCR confirmed that these microRNA were increased. miR-21, a pro-survival miR, was the most significantly increased by mTOR-inhibition (p<0.01). The regulation of miR-21 by Rapamycin is cell type independent. mTOR inhibition promotes the processing of the miR-21 transcript (pri-miR-21) to a premature form (pre-miR-21). In conclusion, our findings demonstrate that Rapamycin upregulates multiple miRs, including pro-survival miRs, in TSC2-deficient patient-derived cells. The induction of miRs may contribute to the response of LAM and TSC patients to Rapamycin therapy. PMID:23555865

  9. Screening antimutagenic and antiproliferative properties of extracts isolated from Jackfruit pulp (Artocarpus heterophyllus Lam).

    PubMed

    Ruiz-Montañez, G; Burgos-Hernández, A; Calderón-Santoyo, M; López-Saiz, C M; Velázquez-Contreras, C A; Navarro-Ocaña, A; Ragazzo-Sánchez, J A

    2015-05-15

    The present focused on the study of the antimutagenic and antiproliferative potential of pulp Jackfruit (Artocarpus heterophyllus Lam) extract, using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line M12.C3.F6 (murine B-cell lymphoma), respectively. Jackfruit pulp extract was sequentially fractionated by chromatography (RP-HPLC) and each fraction was tested for antimutagenic and antiproliferative activities. The organic extracts obtained from Jackfruit pulp reduced the number of revertants caused by aflatoxin B1 (AFB1) and proliferation of cells M12.C3.F6; a dose-response relationship was showed. Sequential RP-HPLC fractionation of the active extracts produced both antimutagenic and/or antiproliferative fractions. These results suggested that the Jackfruit contained compounds with chemoprotective properties to reduce the mutagenicity of AFB1, also proliferation of a cancer cell line. PMID:25577099

  10. Preliminary Assessment of the Chemical Stability of Dried Extracts from Guazuma ulmifolia Lam. (Sterculiaceae).

    PubMed

    Lopes, Gisely C; Longhini, Renata; Dos Santos, Paulo Victor P; Araújo, Adriano A S; Bruschi, Marcos Luciano; de Mello, João Carlos P

    2012-01-01

    We report the results of a preliminary estimation of the stability of the dried extract from bark of Guazuma ulmifolia Lam. ("Mutamba"), with and without added colloidal silicon dioxide (CSD). The physical and chemical properties and the compatibility of CSD in the extract were evaluated for 21 days of storage under stress conditions of temperature (45 ± 2°C) and humidity (75 ± 5%). Thermogravimetry (TG) was supplemented using selective high-performance liquid chromatography (HPLC) for determination of stability of the characteristic constituents (chemical markers), namely, procyanidin B2 (PB2) and epicatechin (EP). The results showed that PB2 is an appropriate compound to be used as a chemical marker in the quality control of dried extracts of G. ulmifolia. The stress study showed that there was no significant difference between the two formulations. However, considering the TG data and the high temperatures involved, the results suggest that CSD increases the stability of the dried extract of G. ulmifolia. PMID:22291706

  11. Preliminary Assessment of the Chemical Stability of Dried Extracts from Guazuma ulmifolia Lam. (Sterculiaceae)

    PubMed Central

    Lopes, Gisely C.; Longhini, Renata; dos Santos, Paulo Victor P.; Araújo, Adriano A. S.; Bruschi, Marcos Luciano; de Mello, João Carlos P.

    2012-01-01

    We report the results of a preliminary estimation of the stability of the dried extract from bark of Guazuma ulmifolia Lam. (“Mutamba”), with and without added colloidal silicon dioxide (CSD). The physical and chemical properties and the compatibility of CSD in the extract were evaluated for 21 days of storage under stress conditions of temperature (45 ± 2°C) and humidity (75 ± 5%). Thermogravimetry (TG) was supplemented using selective high-performance liquid chromatography (HPLC) for determination of stability of the characteristic constituents (chemical markers), namely, procyanidin B2 (PB2) and epicatechin (EP). The results showed that PB2 is an appropriate compound to be used as a chemical marker in the quality control of dried extracts of G. ulmifolia. The stress study showed that there was no significant difference between the two formulations. However, considering the TG data and the high temperatures involved, the results suggest that CSD increases the stability of the dried extract of G. ulmifolia. PMID:22291706

  12. Chemical and sensory comparison of fresh and dried lulo (Solanum quitoense Lam.) fruit aroma.

    PubMed

    Forero, Diana Paola; Orrego, Carlos Eduardo; Peterson, Devin Grant; Osorio, Coralia

    2015-02-15

    The odour-active volatile compounds of lulo fruit (Solanum quitoense Lam.) were isolated by solvent extraction followed by solvent-assisted flavour evaporation (SAFE). GC-O and GCMS analyses as well as quantitation by internal standard method showed that (Z)-3-hexenal, ethyl butanoate, 3-sulphanylhexyl acetate, and ethyl hexanoate were key aroma compounds in this fruit. Other odorants with relevance because their contribution (high OAVs) to the overall aroma were 4-hydroxy-2,5-dimethyl-3(2H)-furanone, methyl benzoate, (E)-2-hexenal, and hexanal. Lulo fruit pulp in presence of maltodextrin DE-20 was dried by using four different types of drying methods: hot air-drying (HD), spray drying (SD), lyophilisation (LD), and ultrasonic convective hot air-drying (HUD). LD sample exhibited the highest sensory rank (lulo-like) in comparison with fresh fruit pulp. Hot-air drying processes (HD and HUD) changed adversely the aroma of lulo fruit pulp. PMID:25236202

  13. A new myrsinol-type diterpene polyester from Euphorbia dracunculoides Lam.

    PubMed

    Wang, Li; Yang, Jing; Chi, Yu-Qian; Ouyang, Wen-Bin; Zang, Zhen; Huang, Sheng-Xiong; Cao, Pei; Zhao, Yong

    2015-08-01

    A new myrsinol-type diterpene polyester, 14-deoxo-3?-O-propinoyl-2?,5?,7?,15?-tetra-O-acetyl-14?-O-benzoyl-myrsinol (1), and its known analogue, 14-deoxo-3?-O-prorionyl-5?,15?-di-O-acetyl-7?-O-nicotinoyl-myrsinol-14?-acetate (2), together with a monoterpenoid, pubinernoid A (3), two indole alkaloids, neoechinulin A (4) and dihydroxyisoechinulin A (5), two benzene derivatives, siringin (6) and (3-methoxyphenyl) acetic acid (7), were isolated from the 70% acetone extract of the aerial parts of Euphorbia dracunculoides Lam. Their structures were elucidated on the basis of spectroscopic evidence and comparison with literature reports. The absolute configuration of 1 was deduced by comparing experimental and calculated ECD spectra. Among them, compounds 4 and 5 were first obtained from the plant source. In addition, the (13)C NMR data of compound 2 was reported for the first time. PMID:25605432

  14. Predicting weed migration from soil and climate maps. [Centaurea maculosa Lam

    SciTech Connect

    Chicoine, T.K.; Fay, P.K.; Nielsen, G.A.

    1985-01-01

    Soil characteristics, elevation, annual precipitation, potential evapotranspiration, length of frost-free season, and mean maximum July temperature were estimated for 116 established infestations of spotted knapweed (Centaurea maculosa Lam. number/sup 3/ CENMA) in Montana using basic land resource maps. Areas potentially vulnerable to invasion by the plant were delineated on the basis of representative edaphic and climatic characteristics. No single environmental variable was an effective predictor of sites vulnerable to invasion by spotted knapweed. Only a combination of variables was effective, indicating that the factors that regulate adaptability of this plant are complex. This technique provides a first approximation map of the regions most similar environmentally to infested sites and; therefore, most vulnerable to further invasion. This weed migration prediction technique shows promise for predicting suitable habitats of other invader species. 6 references, 4 figures, 1 table.

  15. High quality reference genome of drumstick tree (Moringa oleifera Lam.), a potential perennial crop.

    PubMed

    Tian, Yang; Zeng, Yan; Zhang, Jing; Yang, ChengGuang; Yan, Liang; Wang, XuanJun; Shi, ChongYing; Xie, Jing; Dai, TianYi; Peng, Lei; Zeng Huan, Yu; Xu, AnNi; Huang, YeWei; Zhang, JiaJin; Ma, Xiao; Dong, Yang; Hao, ShuMei; Sheng, Jun

    2015-07-01

    The drumstick tree (Moringa oleifera Lam.) is a perennial crop that has gained popularity in certain developing countries for its high-nutrition content and adaptability to arid and semi-arid environments. Here we report a high-quality draft genome sequence of M. oleifera. This assembly represents 91.78% of the estimated genome size and contains 19,465 protein-coding genes. Comparative genomic analysis between M. oleifera and related woody plant genomes helps clarify the general evolution of this species, while the identification of several species-specific gene families and positively selected genes in M. oleifera may help identify genes related to M. oleifera's high protein content, fast-growth, heat and stress tolerance. This reference genome greatly extends the basic research on M. oleifera, and may further promote applying genomics to enhanced breeding and improvement of M. oleifera. PMID:26032590

  16. Somatic embryogenesis and plant regeneration from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino

    Microsoft Academic Search

    Iyyakkannu Sivanesan; Mi Young Lim; Byoung Ryong Jeong

    A simple and efficient protocol was developed for somatic embryogenesis from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino. Somatic embryos (SE) were obtained with greater frequency from petiole explants than from leaf explants when cultured\\u000a on Murashige and Skoog (MS) medium supplemented with 2.0 mg L?1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg L?1 6-benzyladenine (BA). On this medium, a mean

  17. Increased resistance to crown rust disease in transgenic Italian ryegrass ( Lolium multiflorum Lam.) expressing the rice chitinase gene

    Microsoft Academic Search

    Wataru Takahashi; Masahiro Fujimori; Yuichi Miura; Toshinori Komatsu; Yoko Nishizawa; Tadaaki Hibi; Tadashi Takamizo

    2005-01-01

    We introduced the rice chitinase (Cht-2; RCC2) gene into calli of Italian ryegrass (Lolium multiflorum Lam.), with a hygromycin phosphotransferase (HPT) gene as a selectable marker, by particle bombardment. Hygromycin-resistant calli were selected and transferred to regeneration medium for shoot formation. Polymerase chain reaction (PCR) analysis revealed regenerants containing the HPT gene. The RCC2 gene was detected in 65.5% of

  18. Trace element partition coefficients for clinopyroxene and phlogopite in an alkaline lamprophyre from Newfoundland by LAM-ICP-MS

    Microsoft Academic Search

    Stephen F. Foley; Simon E. Jackson; Brian J. Fryer; John D. Greenouch; George A. Jenner

    1996-01-01

    Mineral\\/rock matrix partition coefficients have been determined for clinopyroxene (Cpx) and phlogopite from a Mesozoic alkaline lamprophyre from Newfoundland, Canada, by Laser Ablation Microprobe (LAM-ICP-MS). Values for twenty-one elements were obtained for Cpx, whereas only eleven were possible for phlogopite due to REE abundances below detection limits ( 1 ppm). Ablation pits of 40–50 ?m diameter enabled investigation of zonation

  19. Disentangling the Origins of Cultivated Sweet Potato (Ipomoea batatas (L.) Lam.)

    PubMed Central

    Roullier, Caroline; Duputié, Anne; Wennekes, Paul; Benoit, Laure; Fernández Bringas, Víctor Manuel; Rossel, Genoveva; Tay, David; McKey, Doyle; Lebot, Vincent

    2013-01-01

    Sweet potato (Ipomoea batatas (L.) Lam., Convolvulaceae) counts among the most widely cultivated staple crops worldwide, yet the origins of its domestication remain unclear. This hexaploid species could have had either an autopolyploid origin, from the diploid I. trifida, or an allopolyploid origin, involving genomes of I. trifida and I. triloba. We generated molecular genetic data for a broad sample of cultivated sweet potatoes and its diploid and polyploid wild relatives, for noncoding chloroplast and nuclear ITS sequences, and nuclear SSRs. Our data did not support an allopolyploid origin for I. batatas, nor any contribution of I. triloba in the genome of domesticated sweet potato. I. trifida and I. batatas are closely related although they do not share haplotypes. Our data support an autopolyploid origin of sweet potato from the ancestor it shares with I. trifida, which might be similar to currently observed tetraploid wild Ipomoea accessions. Two I. batatas chloroplast lineages were identified. They show more divergence with each other than either does with I. trifida. We thus propose that cultivated I. batatas have multiple origins, and evolved from at least two distinct autopolyploidization events in polymorphic wild populations of a single progenitor species. Secondary contact between sweet potatoes domesticated in Central America and in South America, from differentiated wild I. batatas populations, would have led to the introgression of chloroplast haplotypes of each lineage into nuclear backgrounds of the other, and to a reduced divergence between nuclear gene pools as compared with chloroplast haplotypes. PMID:23723970

  20. Achyrocline satureioides (LAM.) DC (Marcela): antimicrobial activity on Staphylococcus spp. and immunomodulating effects on human lymphocytes.

    PubMed

    Calvo, D; Cariddi, L N; Grosso, M; Demo, M S; Maldonado, A M

    2006-01-01

    Achyrocline satureioides (LAM.) DC (Compositae) is a sub-bush original from America and distributed in Europe and Africa. It is mainly used in infusions, as digestive, sedative among others and has antimicrobial and antiviral properties. A research was made into the anti-microbial activity of the A. satureioides decoction on the Staphylococcus spp strains. They were isolated from 18 patients with acne lesions and from 7 patients infected with Staphylococcus spp. (5 strains were taken from catheters and 2 from wounds). The strains were classified through biochemical tests and then were seeded in triptein-soy agar with or without decoction to observe the antibacterial activity. On the other hand, cultures of lymphocytes were made from those patients who displayed infections caused by Staphylococcus spp. and from 12 control non-infected individuals. The lymphocytes were stimulated with decoction or PHA-M. Among the expanded, CD8+ T cells, with anti-human CD8 monoclonal antibody were the outstanding ones by indirect IF. The A. satureiodes decoction inhibited 95% of the isolated Staphylococcus spp. strains and stimulated the lymphocyte expansion, of which 40% were CD8+ T cells. The A. satureiodes decoction showed anti-microbial activity and resulted to be an immunostimulating agent on CD8+ T cells, with lesser mitogenic effects than PHA-M. PMID:18293658

  1. Identification of Ellagitannins and Flavonoids from Eugenia brasilienses Lam. (Grumixama) by HPLC-ESI-MS/MS.

    PubMed

    Teixeira, Luciane de Lira; Bertoldi, Fabiano Cleber; Lajolo, Franco Maria; Hassimotto, Neuza Mariko Aymoto

    2015-06-10

    The grumixama (Eugenia brasiliensis Lam.), also known as Brazilian cherry, is a fruit native to Brazil. This study identified the flavonoids in the flesh and seeds and ellagitannin in the flesh of purple and yellow varieties. The physicochemical characteristics and antioxidant capacity of these fruits were also evaluated. Anthocyanins and flavonols were found in high levels in the flesh of purple (32-180 mg 100 g(-1) FW) and yellow grumixama (13-41 mg 100 g(-1) FW), respectively. The major flavonoids identified were cyanidin 3-glucoside and quercetin aglycone. Furthermore, ellagitannins were found in high levels in the flesh of purple (82-243 mg ellagic acid equiv 100 g(-1) FW) and yellow grumixama (92 mg ellagic acid equiv 100 g(-1) FW) and seeds (2220-2905 mg ellagic acid equiv 100 g(-1) FW). The ellagitannin profiles of both varieties were first characterized in which pedunculagin isomers, strictinin isomers, and ellagic acid galloyl hexoside were the major ellagitannins identified. In summary, both varieties of the grumixama fruit as well as the seeds could be good sources of bioactive compounds, mainly ellagitannins. PMID:25990484

  2. Phytochemical Analysis and Metal-chelation Activity of Achillea tenuifolia Lam.

    PubMed Central

    Moradkhani, Shirin; Ayatollahi, Abdul Majid; Ghanadian, Mustafa; Moin, Mohammad Reza; Razavizadeh, Masoud; Shahlaei, Mohsen

    2012-01-01

    Achillea tenuifolia Lam. (Asteraceae) afforded a dichloromethane fraction from which three known compounds ?-sitosterol (compound1), 5-hydroxy, 4',6,7– trimethoxy flavone (salvigenin compound 2), and methyl-gallate (compound 3) were isolated for the first time. The structure of isolated compounds was elucidated by different spectroscopic methods. Applying the molar-ratio method, the complexation of salvigenin with Fe (III), Cu(II) and Zn(II), the most abundant type of metal ions in the body, were then evaluated. It was determined that stoichiometric ratio of salvigenin with these cations were as Fe(Salvigenin)2 (H2O)2 and Cu(Salvigenin)2(H2O)2 in methanolic solution without pH control, while zinc ions didn`t form significant complexes. The results were confirmed more, by computational molecular modeling of the structure of proposed ligand-complexes by semi-imperical PM3 calculations, which determined negative heat of formation for the complexes Fe(III) and Cu(II) ions as -689.7 and -573.5, respectively and proposed chelating affinity of salvigenin in the following order: Fe(III) > Cu(II) >> Zn(II). PMID:24250440

  3. In vitro antioxidant activity of Hedyotis corymbosa (L.) Lam. aerial parts.

    PubMed

    Sasikumar, J M; Maheshu, V; Aseervatham, G Smilin Bell; Darsini, D Teepica Priya

    2010-02-01

    The methanolic extract of the aerial part of Hedyotis corymbosa (L.) Lam. (Rubiaceae) was screened for antioxidant activity using 1,1-diphenyl-2-picryl hydroxyl (DPPH) quenching assay, 2,2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) cation decolorization test, ferric reducing power (FRP), scavenging capacity towards hydroxyl ion (OH*) radicals and nitric oxide (NO) radical inhibition activity using established assay procedures. Total phenolics and total flavonoid contents were, also determined. The plant yielded 210 mg gallic acid equivalent/100 g phenolic content and 55 mg quercetin equivalent/100 g flavonoid content. The extract exhibited high antiradical activity against DPPH, ABTS, nitric oxide and hydroxyl radicals with EC50 value of 82, 150, 130, and 170 microg/ml, respectively. The FRP increased with increasing concentration of the sample. The antioxidant activity of the extract was comparable with that of the standard butylated hydroxyl toluene (BHT). High correlation between total phenolic/flavonoid contents and scavenging potential of different reactive oxygen species (R2 = 0.785-0.998) indicated the polyphenols as the main antioxidants. PMID:21086755

  4. The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication.

    PubMed

    Husin, Fitrien; Chan, Yean Yean; Gan, Siew Hua; Sulaiman, Siti Amrah; Shueb, Rafidah Hanim

    2015-01-01

    Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus. PMID:25767554

  5. The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication

    PubMed Central

    Husin, Fitrien; Chan, Yean Yean; Gan, Siew Hua; Sulaiman, Siti Amrah; Shueb, Rafidah Hanim

    2015-01-01

    Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus. PMID:25767554

  6. Purification and structural characterization of an ?-glucosidase inhibitory polysaccharide from apricot (Armeniaca sibirica L. Lam.) pulp.

    PubMed

    Cui, Jie; Gu, Xin; Wang, Fengjun; Ouyang, Jie; Wang, Jianzhong

    2015-05-01

    In this study, the crude polysaccharide (APPS) from the fruiting bodies of apricot (Armeniaca sibirica L. Lam.) was isolated and fractionated by ultrafiltration and Sephadex G-75 gel chromatography. The hypoglycemic activities of all fractions were determined by ?-glucosidase inhibitory activity in vitro. The fraction APPS1-2 showed the best activity with an IC50 of 6.06 mg/mL. The properties and chemical compositions of this fraction were analyzed with high-performance liquid chromatography, gel permeation chromatography-eighteen angle laser light scattering instrument, UV spectroscopy, infrared spectroscopy, and NMR spectroscopy ((1)H). The results demonstrated that APPS1-2 was a neutral glycoconjugate with a molecular weight of 25.93 kDa. It comprised rhamnose, glucose, mannose, and galactose, with a relative molar ratio of 1.34:2.01:0.48:0.35. The backbone of APPS1-2 may consist of rhamnose and glucose, but its branches may consist of mannose and galactose. The IR and UV spectrum of APPS1-2 revealed the typical characteristics of heteropolysaccharide. (1)H NMR spectrum showed that APPS1-2 contained ?-configurations. PMID:25659703

  7. Investigation on the influence of foreign metal ions in crystal growth and characterization of L-Alaninium Maleate (LAM) single crystals.

    PubMed

    Ruby Nirmala, L; Thomas Joseph Prakash, J

    2013-11-01

    A Nonlinear Optical, good quality, single crystals of doped and undoped l-Alaninium Maleate (LAM) were grown by slow evaporation solution growth technique at room temperature. The lattice parameters were analyzed by single crystal X-ray diffraction technique. The identification of Cadmium ion in the doped crystals was done using the EDAX spectrum. The presence of functional group of the dopant with LAM molecule was studied using FTIR spectra. The results of UV-Vis study is used to compare the transparencies of the doped and undoped LAM crystals. The optical band gap energy of the grown crystal was also calculated. The relative second harmonic generation (SHG) efficiency measurement with KDP reference is used to find the incorporation of metal to l-Alaninium Maleate crystals and the parent material. Also the thermal stability of the grown crystals was studied by TGA/DTA spectrum. The mechanical stability of the grown crystals was confirmed through Vickers micro hardness study. By parallel plate capacitor technique, the dielectric response was studied over a wide range of frequencies at different temperatures. The various studies showed the incorporation of the impurity Cd(2+) into LAM crystals and the investigations indicated that the impurity played an important role in the changes of the spectral and structural properties of LAM crystals. PMID:23892119

  8. Folding studies of Purified LamB Protein, the Maltoporin from the Escherichia coli Outer Membrane: Trimer Dissociation can be separated from Unfolding

    PubMed Central

    Baldwin, Valerie; Bhatia, Mandeep; Luckey, Mary

    2011-01-01

    The folding mechanisms for ?-barrel membrane proteins present unique challenges because acquisition of both secondary and tertiary structure is coupled with insertion into the bilayer. For the porins in Escherichia coli outer membrane, the assembly pathway also includes association into homotrimers. We study the folding pathway for purified LamB protein in detergent and observe extreme hysteresis in unfolding and refolding, as indicated by the shift in intrinsic fluorescence. The strong hysteresis is not seen in unfolding and refolding a mutant LamB protein lacking the disulfide bond, as it unfolds at much lower denaturant concentrations than wild type LamB protein. The disulfide bond is proposed to stabilize the structure of LamB protein by clasping together the two sides of Loop 1 as it lines the inner cavity of the barrel. In addition we find that low pH promotes dissociation of the LamB trimer to folded monomers, which run at about one third the size of the native trimer during SDS PAGE and are much more resistant to trypsin than the unfolded protein. We postulate the loss at low pH of two salt bridges between Loop 2 of the neighboring subunit and the inner wall of the monomer barrel destabilizes the quaternary structure. PMID:21640073

  9. In vitro antibacterial potency of Butea monosperma Lam. against 12 clinically isolated multidrug resistant bacteria

    PubMed Central

    Sahu, Mahesh Chandra; Padhy, Rabindra Nath

    2013-01-01

    Objective To investigate the antibacterial activity, using cold and hot extraction procedures with five solvents, petroleum ether, acetone, ethanol, methanol and water to validate medicinal uses of Butea monosperma Lam (B. monosperma) in controlling infections; and to qualitatively estimate phytochemical constituents of leaf-extracts of the plant. Methods The antibacterial activity of leaf-extracts was evaluated by the agar-well diffusion method against clinically isolated 12 Gram-positive and -negative multidrug resistant (MDR) pathogenic bacteria in vitro. Values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of leaf-extracts against each bacterium were obtained in a 96-well micro-titre plate, by broth dilution micro-titre plate technique. Results The presence of tannins, flavonoids, starch, glycosides and carbohydrates in different leaf extracts was established. Pathogenic bacteria used were, Acinetobacter sp., Chromobacterium violaceum, Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Shigella sp., Enterococcus sp., Staphylococcus aureus (S. aureus), methicillin resistant S. aureus and vancomycin resistant S. aureus, along with standard bacterial strains. These MDR bacteria had been recorded to have significant inhibitions by leaf extracts, obtained by cold and hot extraction procedures with five solvents. In addition, the hot aqueous extract against Enterococcus sp. had the highest inhibition zone-size (21 mm). Ciprofloxacin 30 µg/disc was the positive/reference control and the diluting solvent, 10% dimethyl sulphoxide was the negative control. Recorded MIC values of different extracts ranged between 0.23 and 13.30 mg/mL, and MBC values were 0.52 to 30.00 mg/mL, for these bacteria. Conclusions Leaf-extracts with hot water and ethanol had shown significant antibacterial activity against all bacteria. B. monosperma leaf-extract could be used in treating infectious diseases, caused by the range of tested bacteria, as complementary and alternate medicine.

  10. The aerial parts of Guazuma ulmifolia Lam. protect against NSAID-induced gastric lesions.

    PubMed

    Berenguer, B; Trabadela, C; Sánchez-Fidalgo, S; Quílez, A; Miño, P; De la Puerta, R; Martín-Calero, M J

    2007-11-01

    Guazuma ulmifolia Lam., a member of the Sterculiaceae family, is used in folk medicine because of its antioxidant, antimicrobial and antihypertensive properties. Most of the research work carried out on this plant has focused on the bark because of its high concentration of antioxidant proanthocyanidins. The flowers and leaves of Guazuma ulmifolia, though less studied, are also used as a remedy for different conditions, such as kidney and gastrointestinal diseases, fever and diabetes. The aim of this study was to assess the gastroprotective effects of an aqueous suspension of the ethanolic extract from leaves and flowers of Guazuma ulmifolia in a model of acute gastric ulcer induced by diclofenac as ulcerogenic agent, using the proton pump inhibitor omeprazole as a protection reference. Therefore, the extract was administered two times orally to three groups of Wistar rats at doses of 500, 250 and 125mg/kg, with a 24-h interval between doses. Diclofenac (100mg/kg) was given 1h after the last administration of the extract. Pretreatment with Guazuma ulmifolia or omeprazole decreased the ulcerated area in a dose-dependent way. Myeloperoxidase activity as a marker of neutrophil infiltration was slightly reduced in vivo, whereas in vitro, anti-inflammatory activity was clearly inhibited in a dose-dependent way. The lowest doses of the extract significantly decreased the levels of lipoperoxides, and superoxide dismuthase activity increased to a similar extent as with omeprazole (P<0.001). Examination of glutathione metabolism reflected a significant rise in glutathione peroxidase activity at the highest dose of Guazuma ulmifolia. Finally, there was a faint elevation in prostaglandin E(2) levels with all doses, though the depletion induced by diclofenac could not be reverted. We conclude that the aerial parts of Guazuma ulmifolia protect gastric mucosa against the injurious effect of NSAIDs mainly by anti-inflammatory and radical-scavenging mechanisms. PMID:17884315

  11. Oxygen consumption rate and Na+/K+-ATPase activity in early developmental stages of the sea urchin Paracentrotus lividus Lam.

    NASA Astrophysics Data System (ADS)

    Tomši?, Sanja; Stankovi?, Suzana; Lucu, ?edomil

    2011-09-01

    Changes in oxygen consumption rate and Na+/K+-ATPase activity during early development were studied in the sea urchin Paracentrotus lividus Lam. The oxygen consumption rate increased from 0.12 ?mol O2 mg protein-1 h-1 in unfertilized eggs to 0.38 ?mol O2 mg protein-1 h-1 25 min after fertilization. Specific activity of the Na+/K+-ATPase was significantly stimulated after fertilization, ranging up to 1.07 ?mol Pi h-1 mg protein-1 in the late blastula stage and slightly lower values in the early and late pluteus stages.

  12. Target site mutation and reduced translocation are present in a glyphosate-resistant Lolium multiflorum Lam. biotype from Spain.

    PubMed

    González-Torralva, Fidel; Gil-Humanes, Javier; Barro, Francisco; Brants, Ivo; De Prado, Rafael

    2012-09-01

    The resistance mechanism of a glyphosate-resistant Lolium multiflorum Lam. biotype collected in Córdoba (Southern Spain) was examined. Resistance Factor values at three different growth stages ranged between 4.77 and 4.91. At 96 hours after treatment (HAT) the S biotype had accumulated seven times more shikimic acid than the R biotype. There were significant differences in translocation of (14)C-glyphosate between biotypes, i.e. at 96 HAT, the R biotype accumulated in the treated leaf more than 70% of the absorbed herbicide, in comparison with 59.21% of the S biotype; the R biotype translocated only 14.79% of the absorbed (14)C-glyphosate to roots, while in the S population this value was 24.79%. Visualization of (14)C-glyphosate by phosphor imaging showed a reduced distribution in the R biotype compared with the S. Glyphosate metabolism was not involved in the resistance mechanism due to both biotypes showing similar values of glyphosate at 96 HAT. Comparison of the EPSPS gene sequences between biotypes indicated that the R biotype has a proline 182 to serine amino acid substitution. In short, the resistance mechanism of the L. multiflorum Lam. biotype is due to an impaired translocation of the herbicide and an altered target site. PMID:22771431

  13. Performance Comparison of a Matrix Solver on a Heterogeneous Network Using Two Implementations of MPI: MPICH and LAM

    NASA Technical Reports Server (NTRS)

    Phillips, Jennifer K.

    1995-01-01

    Two of the current and most popular implementations of the Message-Passing Standard, Message Passing Interface (MPI), were contrasted: MPICH by Argonne National Laboratory, and LAM by the Ohio Supercomputer Center at Ohio State University. A parallel skyline matrix solver was adapted to be run in a heterogeneous environment using MPI. The Message-Passing Interface Forum was held in May 1994 which lead to a specification of library functions that implement the message-passing model of parallel communication. LAM, which creates it's own environment, is more robust in a highly heterogeneous network. MPICH uses the environment native to the machine architecture. While neither of these free-ware implementations provides the performance of native message-passing or vendor's implementations, MPICH begins to approach that performance on the SP-2. The machines used in this study were: IBM RS6000, 3 Sun4, SGI, and the IBM SP-2. Each machine is unique and a few machines required specific modifications during the installation. When installed correctly, both implementations worked well with only minor problems.

  14. Two-component Bose gases with one-body and two-body couplings Samuel Lellouch, Tung-Lam Dao, Thomas Koffel, and Laurent Sanchez-Palencia

    E-print Network

    and magnetic fields designed to manipulate the internal states of alkali atoms offer a wide range of possiTwo-component Bose gases with one-body and two-body couplings Samuel Lellouch, Tung-Lam Dao, Thomas the competition between one-body and two-body couplings in weakly-interacting two- component Bose gases

  15. EUCLIDEAN PAIRS AND QUASI-EUCLIDEAN RINGS ADEL ALAHMADI(1), S. K. JAIN(1,2), T. Y. LAM(3), AND A. LEROY(4)

    E-print Network

    André, Leroy

    EUCLIDEAN PAIRS AND QUASI-EUCLIDEAN RINGS ADEL ALAHMADI(1), S. K. JAIN(1,2), T. Y. LAM(3), AND A. LEROY(4) Abstract. We study the interplay between the classes of right quasi-Euclidean rings and right K Euclidean divisions and matrix factorizations into idempotents. Right quasi-Euclidean rings are closed under

  16. An Evaluation of Arctic Cloud and Radiation Processes Simulated by the Limited-Area Version of the Global Multiscale Environmental Model (GEM-LAM)

    Microsoft Academic Search

    Dragan Simjanovski; Eric Girard; Ping Du

    2011-01-01

    Cloud and radiation processes simulated by the limited area version of the Global Environmental Multiscale Model (GEM-LAM) are evaluated for the period September 1997 to October 1998 over the western Arctic Ocean. This period coincides with the Surface Heat Budget of the Arctic Ocean (SHEBA) field experiment. Surface downwelling solar and terrestrial radiation, surface albedo, vertically integrated water vapour, liquid

  17. Perera Lam: An environmental justice assessment of the Mississippi River Industrial Corridor in Louisiana, U.S. APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 11(4): 681-697.

    E-print Network

    in Louisiana, U.S. - 681 - APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 11(4): 681-697. http-BASED APPROACH PERERA, P.K.P.1 * ­ LAM, N.2 1 Institution Department of Forestry and Environmental Science of Environmental Sciences, School of the Coast and Environment, Louisiana State University, Baton Rouge, LA, 70803

  18. Folding studies of purified LamB protein, the maltoporin from the Escherichia coli outer membrane: Trimer dissociation can be separated from unfolding

    Microsoft Academic Search

    Valerie Baldwin; Mandeep Bhatia; Mary Luckey

    2011-01-01

    The folding mechanisms for ?-barrel membrane proteins present unique challenges because acquisition of both secondary and tertiary structure is coupled with insertion into the bilayer. For the porins in Escherichia coli outer membrane, the assembly pathway also includes association into homotrimers. We study the folding pathway for purified LamB protein in detergent and observe extreme hysteresis in unfolding and refolding,

  19. Applications of a Feather-weight Virtual Machine Yang Yu Hariharan Kolam govindarajan Lap-Chung Lam Tzi-cker Chiueh

    E-print Network

    Chiueh, Tzi-cker

    Applications of a Feather-weight Virtual Machine Yang Yu Hariharan Kolam govindarajan Lap-Chung Lam}@cs.sunysb.edu Abstract A Feather-weight Virtual Machine (FVM) is an OS-level virtual- ization technology that enables kernel and allow each VM to run a set of applications isolated from other VMs. Feather-weight Virtual

  20. The ?-Glucanase ZgLamA from Zobellia galactanivorans Evolved a Bent Active Site Adapted for Efficient Degradation of Algal Laminarin*

    PubMed Central

    Labourel, Aurore; Jam, Murielle; Jeudy, Alexandra; Hehemann, Jan-Hendrik; Czjzek, Mirjam; Michel, Gurvan

    2014-01-01

    Laminarinase is commonly used to describe ?-1,3-glucanases widespread throughout Archaea, bacteria, and several eukaryotic lineages. Some ?-1,3-glucanases have already been structurally and biochemically characterized, but very few from organisms that are in contact with genuine laminarin, the storage polysaccharide of brown algae. Here we report the heterologous expression and subsequent biochemical and structural characterization of ZgLamAGH16 from Zobellia galactanivorans, the first GH16 laminarinase from a marine bacterium associated with seaweeds. ZgLamAGH16 contains a unique additional loop, compared with other GH16 laminarinases, which is composed of 17 amino acids and gives a bent shape to the active site cleft of the enzyme. This particular topology is perfectly adapted to the U-shaped conformation of laminarin chains in solution and thus explains the predominant specificity of ZgLamAGH16 for this substrate. The three-dimensional structure of the enzyme and two enzyme-substrate complexes, one with laminaritetraose and the other with a trisaccharide of 1,3–1,4-?-d-glucan, have been determined at 1.5, 1.35, and 1.13 ? resolution, respectively. The structural comparison of substrate recognition pattern between these complexes allows the proposition that ZgLamAGH16 likely diverged from an ancestral broad specificity GH16 ?-glucanase and evolved toward a bent active site topology adapted to efficient degradation of algal laminarin. PMID:24337571

  1. Quantity and potential biological activity of caffeic acid in sweet potato [Ipomoea batatas (L.) Lam.] storage root periderm.

    PubMed

    Harrison, Howard F; Peterson, Joseph K; Snook, Maurice E; Bohac, Janice R; Jackson, D Michael

    2003-05-01

    The caffeic acid content of storage root periderm and cortex tissues of genetically diverse sweet potato [Ipomoea batatas (L.) Lam.] cultivars and breeding clones was quantified by high-performance liquid chromatography. Periderm caffeic acid content of the clones ranged from 0.008 to 7.97 mg/g dry weight, whereas the highest cortex content was 0.047 mg/g. Clones varied greatly in periderm caffeic acid content in all experiments, but there were also differences between experiments in content averaged for all clones. This indicates that periderm caffeic acid content is subject to genetic and environmental influences. Caffeic acid inhibited the growth of four sweet potato pathogenic fungi and germination of proso millet seeds in bioassays. Inhibitory activity in the bioassays suggests that high periderm caffeic acid levels contribute to the storage root defense chemistry of some sweet potato genotypes. PMID:12720375

  2. [Comparative characteristics of the antioxidant glutathione complex in the Black Sea molluscs Mytilus galloprovincialis Lam. and Anadata inaequivalis Br].

    PubMed

    Gostiukhina, O L

    2012-01-01

    Peculiarities of distribution of lipid peroxidation (LPO) products, glutathione (GSH) level, and antioxidant enzymes - glutathione peroxidase (GP) and glutathione reductase (GR) - in tissues of the Black Sea bivalve molluscs, Anadata inaequivalis Br. (anadara) and Mytilus galloprovincialis Lam. (mussel, black morph) were studied as well as their comparative characteristics were presented. There were established differences in organization of the glutathione organization and in the LPO intensity in tissues of these mollusc species. In all anadara tissues the intensity of LPO processes was lower than that in Mytilus galloprovincialis. The GP activity in hepatopancreas and gills in mussels was significantly higher than in anadara. On the contrary, in foot the GP activity and the GSH content in anadara exceeded essentially those in mussel. The revealed differences might reflect peculiarities of metabolism orientation in tissues of anadara and mussel as well as effect habitat conditions on them. PMID:23136750

  3. Genetic diversity and relationships in cultivars of Lolium multiflorum Lam. using sequence-related amplified polymorphism markers.

    PubMed

    Huang, L K; Jiang, X Y; Huang, Q T; Xiao, Y F; Chen, Z H; Zhang, X Q; Miao, J M; Yan, H D

    2014-01-01

    Sequence-related amplified polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multiflorum Lam.). Eighteen SRAP primer combinations generated 334 amplification bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1) to 0.5000 (me5 + em7), with an average of 0.4921. The genetic similarity coefficient ranged from 0.4304 to 0.8529, and coefficients between 0.65 and 0.90 accounted for 90.00%. The cluster analysis separated the accessions into five groups partly according to their germplasm resource origins. PMID:25501225

  4. Anthelmintic efficacy of aqueous extract of Butea monosperma (Lam.) Kuntze against Haemonchus contortus of sheep and goats.

    PubMed

    Singh, G; Singh, Rajeev; Verma, P K; Singh, R; Anand, A

    2015-06-01

    Infection with Haemonchus contortus is one of the most important economic problems in small ruminants worldwide. Resistance development by parasites, drug residues in meat, toxicity, non-availability and high cost limit the usefulness of currently used synthetic drugs. Therefore, the present study was undertaken to evaluate in vitro anthelmintic efficacy of aqueous extract of seeds of Butea monosperma (Lam.) Kuntze against H. contortus. Phytochemical analysis of extract showed high concentration of phenolic (11.93 ± 0.64 mg of GAE/g of extract), flavonoids (238.17 ± 19.14 mg of quercetin/g extract) and tannin (10.80 ± 0.70 mg of GAE/g of extract) content. The observations revealed that parasites were sluggish and movement was little at 4 h post exposure of 25, 50 mg/ml and very sluggish in 100 mg/ml concentration. The extract showed complete mortality of the adult H. contortus worms at the concentrations of 100 mg/ml at the time exposure of 6 h and with the concentration of 50 mg/ml at the post exposure of 8 h. At 25 mg/ml concentration 50 % mortality was recorded at 6 h and complete at 8 h post exposure. The LC50 at 6 and 8 h were 45.20 and 17.50 mg/ml respectively. Levamisole at concentration of 0.5 mg/ml caused 50 % mortality at 2 h post exposure and full mortality at 4 h post exposure. These cidal effects may be due to presence of high phenolic, flavonoids and tannin content in the extract. The results confirm the aqueous extract of B. monosperma (Lam.) Kuntze on adult H. contortus worms. PMID:26064000

  5. Predominance of multi-drug-resistant LAM and Beijing family strains among Mycobacterium tuberculosis isolates recovered from prison inmates in Tula Region, Russia.

    PubMed

    Ignatova, Anna; Dubiley, Svetlana; Stepanshina, Valentina; Shemyakin, Igor

    2006-10-01

    The genotypic characteristics and drug susceptibility profiles of clinical isolates of Mycobacterium tuberculosis recovered from prison hospital patients in the Tula region (central Russia) during 2001 and 2002 are reported. The emergence of multi-drug-resistant tuberculosis (TB) poses a major health risk to the population, with economic implications for TB control. Prisons serve as a continuous source of TB transmission. The results showed that members of the LAM and Beijing families are major contributors to the epidemiological picture of TB in the population studied. The two families of strains accounted for most of the drug-resistant TB in the population. The genotypic characteristics of the M. tuberculosis predominant LAM strain that was responsible for 31 % of TB cases in this setting are presented. PMID:17005791

  6. In vitro reduction of trypsin inhibitor by purified NADPH\\/thioredoxin system from sprouts of sweet potato ( Ipomoea batatas (L) Lam.) storage roots

    Microsoft Academic Search

    Dong-Jiann Huang; Hsien-Jung Chen; Wen-chi Hou; Tzeng-Err Chen; Yaw-Huei Lin

    2004-01-01

    Sweet potato (Ipomoea batatas (L.) Lam) storage roots were sprouted in the dark for two months, and the sprouts were used as source materials for purification of thioredoxin reductase successively via the DE-52, Sephadex G-75, and 2?,5?-ADP Sepharose 4B column chromatography. Thioredoxin reductases with a molecular mass of ca 33kDa were obtained. The purification was 813-fold and the yield was

  7. The binding pattern of two carbohydrate- binding modules of laminarinase Lam16A from Thermotoga neapolitana: differences in b-glucan binding within family CBM4

    Microsoft Academic Search

    Vladimir V. Zverlov; Ilia Y. Volkov; Galina A. Velikodvorskaya; Wolfgang H. Schwarz

    Carbohydrate-binding modules (CBMs) are often part of the complex hydrolytic extracellular enzymes from bacteria and may modulate their catalytic activity. The thermostable catalytic domain of laminarinase Lam16A from Thermotoga neapolitana (glycosyl hydrolase family 16) is flanked by two CBMs, 148 and 161 aa long. They share a sequence identity of 30%, are homologous to family CBM4 and are thus called

  8. The influence of deep frying using various vegetable oils on acrylamide formation in sweet potato (Ipomoea batatas L. Lam) chips.

    PubMed

    Lim, P K; Jinap, S; Sanny, M; Tan, C P; Khatib, A

    2014-01-01

    The objective of this study was to evaluate the precursors of acrylamide formation in sweet potato (SP) (Ipomoea batatas L. Lam) chips and to determine the effect of different types of vegetable oils (VOs), that is, palm olein, coconut oil, canola oil, and soya bean oil, on acrylamide formation. The reducing sugars and amino acids in the SP slices were analyzed, and the acrylamide concentrations of SP chips were measured. SP chips that were fried in a lower degree of unsaturation oils contained a lower acrylamide concentration (1443 ?g/kg), whereas those fried with higher degree of unsaturated oils contained a higher acrylamide concentration (2019 ?g/kg). SP roots were found to contain acrylamide precursors, that is, 4.17 mg/g glucose and 5.05 mg/g fructose, and 1.63 mg/g free asparagine. The type of VO and condition used for frying, significantly influenced acrylamide formation. This study clearly indicates that the contribution of lipids in the formation of acrylamide should not be neglected. PMID:24344977

  9. Enhanced biofuel production from high-concentration bioethanol wastewater by a newly isolated heterotrophic microalga, Chlorella vulgaris LAM-Q.

    PubMed

    Xie, Tonghui; Liu, Jing; Du, Kaifeng; Liang, Bin; Zhang, Yongkui

    2013-10-28

    Microalgal biofuel production from wastewater has economic and environmental advantages. This article investigates the lipid production from high chemical oxygen demand (COD) bioethanol wastewater without dilution or additional nutrients, using a newly isolated heterotrophic microalga, Chlorella vulgaris LAM-Q. To enhance lipid accumulation, the combined effects of important operational parameters were studied via response surface methodology. The optimal conditions were found to be temperature of 22.8?, initial pH of 6.7, and inoculum density of 1.2 × 10(8) cells/ml. Under these conditions, the lipid productivity reached 195.96 mg/l/d, which was markedly higher than previously reported values in similar systems. According to the fatty acid composition, the obtained lipids were suitable feedstock for biodiesel production. Meanwhile, 61.40% of COD, 51.24% of total nitrogen, and 58.76% of total phosphorus were removed from the bioethanol wastewater during microalgal growth. In addition, 19.17% of the energy contained in the wastewater was transferred to the microalgal biomass in the fermentation process. These findings suggest that C. vulgaris LAMQ can efficiently produce lipids from high-concentration bioethanol wastewater, and simultaneously performs wastewater treatment. PMID:23801252

  10. (±)-catechin, a root exudate of the invasive centaurea stoebe lam. (Spotted knapweed) exhibits bacteriostatic activity against multiple soil bacterial populations.

    PubMed

    Pollock, Jarrod L; Kogan, Lewis A; Thorpe, Andrea S; Holben, William E

    2011-09-01

    Understanding the effects of allelopathic plant chemicals on soil microorganisms is critical to understanding their ecological roles and importance in exotic plant invasion. Centaurea stoebe Lam. (spotted knapweed), an aggressive invasive weed in North America, secretes a racemic mixture of (±)-catechin as a root exudate. This enantiomeric, polyphenolic compound has been reported to have allelopathic effects on surrounding flora and microflora. To better understand how catechin affects microbial communities in the root zone of spotted knapweed, we assessed its impact on the total culturable bacterial component and numerous individual bacterial populations from Romanian (native range) and Montana (invaded range) soils. Catechin suppressed total culturable count numbers from the bacterial community and inhibited growth of some, but not all, soil bacterial populations tested. The native soil bacterial community was significantly more resistant to inhibitory effects of catechin than either the invaded or non-invaded soils. We further show that the inhibitory effect of catechin on nine different soil bacterial strains from seven genera was reversible, demonstrating that it acts via a bacteriostatic rather than bactericidal mechanism. These findings suggest that catechin might affect bacterial community composition and activity in the root zone. PMID:21882071

  11. Immunogenicity of viral B-cell epitopes inserted into two surface loops of the Escherichia coli K12 LamB protein and expressed in an attenuated aroA strain of Salmonella typhimurium.

    PubMed

    Wang, J; Michel, V; Leclerc, C; Hofnung, M; Charbit, A

    1999-01-01

    We previously developed a general procedure which allows the genetic coupling of a chosen foreign linear epitope in different 'permissive' sites of a carrier protein. By using the outer membrane protein LamB of Escherichia coli K12 as a carrier, we were able to express a number of different foreign epitopes at the bacterial surface. In the present work, taking advantage of the recent determination of the crystal structure of LamB, we inserted two model B-cell epitopes i.e.--the C3 epitope from poliovirus (residues 93 to 103 of VP1) and the preS2 epitope from hepatitis B virus, (residues 132 to 145)--at the tip of the most distal and largest surface exposed region of LamB (after residues 386, into loop L9). We also used two previously constructed LamB hybrids, corresponding to the insertion of the C3B or preSB epitope into permissive site 153 (lying in the middle of the fourth surface loop of LamB), to construct two LamB proteins corresponding to the simultaneous insertion of the two different epitopes (with one epitope per site). The LamB hybrids were placed under the control of the anaerobically inducible pnirB promoter and expressed in a LamB-negative derivative of the aroA attenuated strain of S. typhimurium, SL3261. In vitro, the recombinant proteins were expressed at a high level (up to 10% of whole cell proteins) and in vivo the recombinant plasmids were stably maintained. For both epitopes, genetic coupling at site 386 appeared to be more favorable for the induction of anti-epitope antibodies than coupling at site 153. Moreover, the LamB hybrid corresponding to the simultaneous insertion of the preSB epitope at site 153 and of the C3B epitope at site 386 allowed the induction of both anti-poliovirus and anti-hepatitis B antibodies. PMID:10078601

  12. The Saharan heat low and moisture transport pathways in the central Sahara—Multiaircraft observations and Africa-LAM evaluation

    NASA Astrophysics Data System (ADS)

    Engelstaedter, S.; Washington, R.; Flamant, C.; Parker, D. J.; Allen, C. J. T.; Todd, M. C.

    2015-05-01

    We present a characterization of the Saharan heat low (SHL) based on dropsonde observations made on 22 June 2011 by two simultaneously flying aircraft during the Fennec project. The observations are used to identify moisture transport pathways and to validate the UK Met Office limited area model for northern Africa (Africa-LAM). The observations capture the SHL, harmattan, and monsoon surge. The SHL has a northeast-southwest orientated elongated shape centered over northern Mauritania. The SHL core is associated with a 950 hPa temperature minimum (36.4°C) in the morning caused by the monsoon surge and a maximum (42.6°C) in the afternoon. The monsoon surge east of the SHL core splits into two transport pathways: (a) curving around the SHL core in the north, especially pronounced in a morning near-surface layer, and (b) northeastward transport within the ~2 km deep monsoon surge (afternoon observations only). In the morning the model forecasts the harmattan, monsoon surge, and the SHL geographic location and northeast-southwest orientation well but the model represents the SHL flatter and more spatially extended and overestimates the convective boundary layer (CBL) by up to ~0.3 km. The simulated afternoon SHL location appears shifted westward by up to ~1°. The model overestimates the shallow afternoon monsoon surge CBL depth of ~1.8 km by >2 km resulting in southwestward transport of vertically mixed moisture above ~2.5 km contrasting observed northeastward-only transport at lower levels. This moisture distribution model error is likely to have consequences for simulations of Saharan thermodynamics and dust emissions caused by convection-driven cold pools.

  13. Anti-inflammatory effect of Moringa oleifera Lam. seeds on acetic acid-induced acute colitis in rats

    PubMed Central

    Minaiyan, Mohsen; Asghari, Gholamreza; Taheri, Diana; Saeidi, Mozhgan; Nasr-Esfahani, Salar

    2014-01-01

    Objective: Anti-inflammatory, immuno-modulatory, and antioxidant properties of Moringa oleifera Lam. suggest that it might have beneficial effects on colitis. The present study was performed to investigate the anticolitis effect of Moringa oleifera seeds hydro-alcoholic extract (MSHE) and its chloroform fraction (MCF) on acetic acid-induced colitis in rats. Materials and Methods: Both MSHE and MCF with three increasing doses (50, 100, and 200 mg/kg) were administered orally to separate groups of male Wistar rats, 2 h before ulcer induction (using acetic acid 4%) and continued for 5 days. Prednisolone (4 mg/kg) and normal saline (1 ml/kg) were used in reference and control groups, respectively. All rats were sacrificed 24 h after the last dose (at day 6) and tissue injuries were assessed macroscopically and pathologically. Results: Extracts with three doses mentioned before were effective to reduce weight of distal colon (8 cm) as a marker for inflammation and tissue edema. Three doses of MSHE and two greater doses of MCF (100 and 200 mg/kg) were effective to reduce ulcer severity, area, and index as well as mucosal inflammation severity and extent, crypt damage, invasion involvement, total colitis index, and MPO activity compared with controls. MCF (50 mg/kg) was not significantly effective in reducing evaluated parameters of colitis compared with controls. Conclusion: It is concluded that MSHE and MCF were both effective to treat experimental colitis and this might be attributed to their similar major components, biophenols and flavonoids. Since the efficacy was evident even in low doses of MSHE, presence of active constituents with high potency in seeds is persuasive. PMID:25050310

  14. Isolation, characterization and antimicrobial evaluation of a novel compound N-octacosan 7? ol, from Fumaria parviflora Lam

    PubMed Central

    2014-01-01

    Background Fumaria parviflora Lam. (Fumaraceae) is widely used in traditional as well as folkloric system of medicine from ancient. It is commonly known as ‘Pitpapra’ or ‘Shahtrah’ in Indian traditional system of medicine and used for treating numerous ailments like diarrhea, fever, influenza, blood purifier and other complications. The object of the present study was to evaluate the Antileishmanial, antibacterial, antifungal and cytotoxic potential of isolated compound. Methods Methanolic extract of whole plant of Fumaria parviflora was dried under reduced pressure to obtain a dark brown residue which was adsorbed on silica gel column grade (60–120 mesh) to obtain a slurry and chromatographed over silica gel loaded column in petroleum ether – chloroform (3:1, 1:1 and 1:3?v/v). The in vitro antileishmanial evaluation of isolated compound against Leishmania donovani promastigotes was investigated by growth kinetics assay, reversibility assay, analysis of cellular morphology, adverse toxicity and determination of 50% growth inhibitory concentration (GI50). Disc diffusion and broth micro dilution methods were used to study the antibacterial (Gram?+?Staphylococcus epidermidis and Bacillus subtilis; Gram - Escherichia coli and Salmonella typhimurium) and antifungal (Candida albicans and Aspergillus niger) potential in vitro. Results Structure elucidation by spectral data analysis revealed a novel compound, n-octacosan-7?-ol (OC), yield (0.471%), having significant antimicrobial activity against Leishmania donovani promastigotes, Staphylococcus epidermidis, Escherichia coli, Candida albicans and Aspergillus niger in vitro with GI50?=?5.35, MIC 250, MIC 250 and MFC 500 and MIC 250 ?g ml-1 respectively. The isolated compound did not show adverse effect against mammalian macrophages. Conclusions The available evidence of compound suggested that it may be used as antimicrobial agent in future and may provide new platform for drug discovery programmes for leishmaniasis. PMID:24621260

  15. Toddaculin, Isolated from of Toddalia asiatica (L.) Lam., Inhibited Osteoclastogenesis in RAW 264 Cells and Enhanced Osteoblastogenesis in MC3T3-E1 Cells

    PubMed Central

    Watanabe, Akio; Kumagai, Momochika; Mishima, Takashi; Ito, Junya; Otoki, Yurika; Harada, Teppei; Kato, Tsuyoshi; Yoshida, Mikihiko; Suzuki, Misora; Yoshida, Izumi; Fujita, Kazuhiro; Watai, Masatoshi; Nakagawa, Kiyotaka; Miyazawa, Teruo

    2015-01-01

    Osteoporosis with bone loss is widely recognized as a major health problem. Bone homeostasis is maintained by balancing bone formation and bone resorption. The imbalance caused by increased bone resorption over bone formation can lead to various bone-related diseases such as osteoporosis and rheumatoid arthritis. Osteoclasts are the principal cells responsible for bone resorption and the main targets of anti-resorptive therapies. However, excessive inhibition of osteoclast differentiation may lead to inhibition of osteoblast differentiation. Therefore, it is important to screen for new compounds capable of inhibiting bone resorption and enhancing bone formation. Toddalia asiatica (L.) Lam. has been utilized traditionally for medicinal purposes such as the treatment of rheumatism. Currently, the extract is considered to be a good source of pharmacological agents for the treatment of bone-related diseases, but the active compounds have yet to be identified. We investigated whether toddaculin, derived from Toddalia asiatica (L.) Lam., affects both processes by inhibiting bone resorption and enhancing bone formation. Towards this end, we used pre-osteoclastic RAW 264 cells and pre-osteoblastic MC3T3-E1 cells. We found that toddaculin not only inhibited the differentiation of osteoclasts via activation of the NF-?B, ERK 1/2, and p38 MAPK signaling pathways, but it also induced differentiation and mineralization of osteoblasts by regulating differentiation factors. Thus, toddaculin might be beneficial for the prevention and treatment of osteoporosis. PMID:25993011

  16. Suppression of seed bruchid ( Callosobruchus maculatus F.) development and damage on cowpea ( Vigna unguiculata (L.) Walp.) with Zanthoxylum zanthoxyloides (Lam.) Waterm. (Rutaceae) root bark powder when compared to neem seed powder and pirimiphos-methyl

    Microsoft Academic Search

    E. O. Ogunwolu; A. T. Odunlami

    1996-01-01

    Reproduction suppression properties in Zanthoxylum zanthoxyloides (Lam.) Waterm. (Rutaceae) root bark powder (ZRBP) against the cowpea seed bruchid (CSB), Callosobruchus maculatus F., were evaluated against neem (Azadirachta indica A. Juss.) seed powder (NSP) and pirimiphos-methyl (PM) (Actellic 2% Dust, ICI). At application rates ranging from 0.125 to 3 g per 20 g seed, ZRBP was as effective an oviposition suppressant

  17. Ethnobotanical studies on Berberis aristata DC. root extracts

    Microsoft Academic Search

    M. Shahid; T. Rahim; A. Shahzad; A. Latif; T. Fatma; M. Rashid; Adil Raza; S. Mustafa

    2009-01-01

    -3 mg\\/ml and for Aspergillus species, it was 3 ? 10 -3 mg\\/ml. All three extracts also had antifungal activity against the fungal species tested, except Candida krusei. The extracts of B. aristata also demonstrated anti-inflammatory, analgesic, and antipyretic activities. Chemical analysis revealed the presence of alkaloids, amino acids, tannins, terpenes, resins, phenols and reducing sugars as major compounds. FTIR-spectral

  18. Evidence of higher photosynthetic plasticity in the early successional Guazuma ulmifolia Lam. compared to the late successional Hymenaea courbaril L. grown in contrasting light environments.

    PubMed

    Portes, M T; Damineli, D S C; Ribeiro, R V; Monteiro, J A F; Souza, G M

    2010-02-01

    The present study investigated changes in photosynthetic characteristics of Guazuma ulmifolia Lam. (early successional species) and Hymenaea courbaril L. (late successional species) grown in contrasting light conditions as a way of assessing photosynthetic plasticity. Early successional species typically inhabit gap environments being exposed to variability in multiple resources, hence it is expected that these species would show higher photosynthetic plasticity than late successional ones. In order to test this hypothesis, light and CO2 response curves and chlorophyll content (Chl) were measured in plants grown in high and low light environments. G. ulmifolia presented the highest amounts of both Chl a and b, especially in the low light, and both species presented higher Chl a than b in both light conditions. The Chl a/b ratio was higher in high light leaves of both species and greater in G. ulmifolia. Taken together, these results evidence the acclimation potential of both species, reflecting the capacity to modulate light harvesting complexes according to the light environment. However, G. ulmifolia showed evidence of higher photosynthetic plasticity, as indicated by the greater amplitude of variation on photosynthetic characteristics between environments shown by more significant shade adjusted parameters (SAC) and principal component analysis (PCA). Thus, the results obtained were coherent with the hypothesis that the early successional species G. ulmifolia exhibits higher photosynthetic plasticity than the late successional species H. courbaril. PMID:20231962

  19. In vitro antioxidant and antihyperlipidemic activities of Toddalia asiatica (L) Lam. leaves in Triton WR-1339 and high fat diet induced hyperlipidemic rats.

    PubMed

    Irudayaraj, Santiagu Stephen; Sunil, Christudas; Duraipandiyan, Veeramuthu; Ignacimuthu, Savarimuthu

    2013-10-01

    The present study was undertaken to evaluate the in vitro antioxidant and antihyperlipidemic activity of Toddalia asiatica (L) Lam. leaves in Triton WR-1339 and high fat diet-induced hyperlipidemic rats. In in vitro studies T. asiatica leaves ethyl acetate extract showed very good scavenging activity on 2,2-diphenyl-picrylhydrazyl (DPPH) (IC?? 605.34±2.62 ?g/ml), hydroxyl (IC?? 694.37±2.12 ?g/ml) and nitric oxide (IC?? 897.83±1.48 ?g/ml) radicals, as well as high reducing power. In Triton WR-1339 induced hyperlipidemic rats, oral treatment with T. asiatica leaves ethyl acetate extract produced a significant (P?0.005) decrease in the levels of serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein-cholesterol (LDL-C), and significant increase in high-density lipoprotein cholesterol (HDL-C) in comparison with hexane and methanol extracts. In high fat diet-fed hyperlipidemic rats, the ethyl acetate extract (200 and 400 mg/kg) significantly altered the plasma and liver lipids levels to near normal. PMID:23891761

  20. Do ecological niche model predictions reflect the adaptive landscape of species?: a test using Myristica malabarica Lam., an endemic tree in the Western Ghats, India.

    PubMed

    Nagaraju, Shivaprakash K; Gudasalamani, Ravikanth; Barve, Narayani; Ghazoul, Jaboury; Narayanagowda, Ganeshaiah Kotiganahalli; Ramanan, Uma Shaanker

    2013-01-01

    Ecological niche models (ENM) have become a popular tool to define and predict the "ecological niche" of a species. An implicit assumption of the ENMs is that the predicted ecological niche of a species actually reflects the adaptive landscape of the species. Thus in sites predicted to be highly suitable, species would have maximum fitness compared to in sites predicted to be poorly suitable. As yet there are very few attempts to address this assumption. Here we evaluate this assumption. We used Bioclim (DIVA GIS version 7.3) and Maxent (version 3.3.2) to predict the habitat suitability of Myristica malabarica Lam., an economically important tree occurring in the Western Ghats, India. We located populations of the trees naturally occurring in different habitat suitability regimes (from highly suitable to poorly suitable) and evaluated them for their regeneration ability and genetic diversity. We also evaluated them for two plant functional traits, fluctuating asymmetry--an index of genetic homeostasis, and specific leaf weight--an index of primary productivity, often assumed to be good surrogates of fitness. We show a significant positive correlation between the predicted habitat quality and plant functional traits, regeneration index and genetic diversity of populations. Populations at sites predicted to be highly suitable had a higher regeneration and gene diversity compared to populations in sites predicted to be poor or unsuitable. Further, individuals in the highly suitable sites exhibited significantly less fluctuating asymmetry and significantly higher specific leaf weight compared to individuals in the poorly suitable habitats. These results for the first time provide an explicit test of the ENM with respect to the plant functional traits, regeneration ability and genetic diversity of populations along a habitat suitability gradient. We discuss the implication of these results for designing viable species conservation and restoration programs. PMID:24312402

  1. Distributions, ex situ conservation priorities, and genetic resource potential of crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas].

    PubMed

    Khoury, Colin K; Heider, Bettina; Castañeda-Álvarez, Nora P; Achicanoy, Harold A; Sosa, Chrystian C; Miller, Richard E; Scotland, Robert W; Wood, John R I; Rossel, Genoveva; Eserman, Lauren A; Jarret, Robert L; Yencho, G C; Bernau, Vivian; Juarez, Henry; Sotelo, Steven; de Haan, Stef; Struik, Paul C

    2015-01-01

    Crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, the limited availability of germplasm with which to perform crosses, and the difficulty of introgression of genes from wild species has constrained their utilization. Here, we compile geographic occurrence data on relevant sweetpotato wild relatives and produce potential distribution models for the species. We then assess the comprehensiveness of ex situ germplasm collections, contextualize these results with research and breeding priorities, and use ecogeographic information to identify species with the potential to contribute desirable agronomic traits. The fourteen species that are considered the closest wild relatives of sweetpotato generally occur from the central United States to Argentina, with richness concentrated in Mesoamerica and in the extreme Southeastern United States. Currently designated species differ among themselves and in comparison to the crop in their adaptations to temperature, precipitation, and edaphic characteristics and most species also show considerable intraspecific variation. With 79% of species identified as high priority for further collecting, we find that these crop genetic resources are highly under-represented in ex situ conservation systems and thus their availability to breeders and researchers is inadequate. We prioritize taxa and specific geographic locations for further collecting in order to improve the completeness of germplasm collections. In concert with enhanced conservation of sweetpotato wild relatives, further taxonomic research, characterization and evaluation of germplasm, and improving the techniques to overcome barriers to introgression with wild species are needed in order to mobilize these genetic resources for crop breeding. PMID:25954286

  2. Eugenia jambolana Lam. Berry Extract Inhibits Growth and Induces Apoptosis of Human Breast Cancer but not Non-Tumorigenic Breast Cells

    PubMed Central

    Li, Liya; Adams, Lynn S.; Chen, Shiuan; Killian, Caroline; Ahmed, Aftab; Seeram, Navindra P.

    2009-01-01

    The ripe purple berries of the native Indian plant, Eugenia jambolana Lam., known as Jamun, are popularly consumed and available in the United States in Florida and Hawaii. Despite the growing body of data on the chemopreventive potential of edible berry extracts, there is paucity of such data for Jamun fruit. Therefore our laboratory initiated the current study with the following objectives:1) to prepare a standardized Jamun fruit extract (JFE) for biological studies and, 2) to investigate the anti-proliferative and pro-apoptotic effects of JFE in estrogen dependent/aromatase positive (MCF-7aro), and estrogen independent (MDA-MB-231) breast cancer cells, and in a normal/non-tumorigenic (MCF-10A) breast cell line. JFE was standardized to anthocyanin content using the pH differential method, and individual anthocyanins were identified by high performance liquid chromatography with ultraviolet (HPLC-UV) and tandem mass spectrometry (LC-MS/MS) methods. JFE contained 3.5% anthocyanins (as cyanidin-3-glucoside equivalents) which occur as diglucosides of five anthocyanidins/aglycons: delphinidin, cyanidin, petunidin, peonidin and malvidin. In the proliferation assay, JFE was most effective against MCF-7aro (IC50=27 µg/mL), followed by MDA-MB-231 (IC50=40 µg/mL) breast cancer cells. Importantly, JFE exhibited only mild antiproliferative effects against the normal MCF-10A (IC50>100 µg/mL) breast cells. Similarly, JFE (at 200 µg/mL) exhibited pro-apoptotic effects against the MCF-7aro (p?0.05) and the MDA-MB-231 (p?0.01) breast cancer cells, but not towards the normal MCF-10A breast cells. These studies suggest that JFE may have potential beneficial effects against breast cancer. PMID:19166352

  3. Please cite this article in press as: Bradley, C.R., et al., Conservation genetics of Ireland's sole population of the River water crowfoot (Ranunculus fluitans Lam.). Aquat. Bot. (2013), http://dx.doi.org/10.1016/j.aquabot.2013.01.011

    E-print Network

    Provan, Jim

    2013-01-01

    Please cite this article in press as: Bradley, C.R., et al., Conservation genetics of Ireland.elsevier.com/locate/aquabot Conservation genetics of Ireland's sole population of the River water crowfoot (Ranunculus fluitans Lam's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, United Kingdom a r t i c l e i n f o

  4. Heat capacities and thermodynamic functions of new cobalt manganites LaM{2/II}CoMnO6 (MII-Mg, Ca, Sr, Ba) in the 298.15-673 K temperature range

    NASA Astrophysics Data System (ADS)

    Kasenov, B. K.; Turtubaeva, M. O.; Amerkhanova, Sh. K.; Kasenova, Sh. B.; Sagintaeva, Zh. I.; Kuanyshbekov, E. E.; Seisenova, A. A.; Smagulova, D. I.

    2015-06-01

    Temperature dependences of the heat capacities of cobalt manganites LaM{2/II}CoMnO6 (MII = Mg, Ca, Sr, Ba) are investigated by means of dynamic calorimetry in the temperature range of 298.15-673 K. It is established that cobalt manganites display ?-like effects on the C {/p po} ˜ f( T) curve, which are likely to be related to the type II phase transitions. Equations for the temperature dependence of the heat capacity of cobalt manganites are derived from the experimental data with allowance for phase transition temperatures. The values of C {/p po}( T) and thermodynamic functions H po( T) - H po(298.15), S po( T), and ?xx( T) are calculated.

  5. Phylogenetic diversity of rhizobia associated with horsegram [Macrotyloma uniflorum (Lam.) Verdc.] grown in South India based on glnII, recA and 16S-23S intergenic sequence analyses.

    PubMed

    Appunu, Chinnaswamy; Ganesan, Govindan; Kalita, Micha?; Kaushik, Raghavan; Saranya, Balamurugan; Prabavathy, Vaiyapuri Ramalingam; Sudha, Nair

    2011-04-01

    Horsegram [Macrotyloma uniflorum (Lam.) Verdc.) is an important grain legume and fodder crop in India. Information on root nodule endosymbionts of this legume in India is limited. In the present study, 69 isolates from naturally occurring root nodules of horsegram collected from two agro-eco-climatic regions of South India was analyzed by generation rate, acid/alkali reaction on YMA medium, restriction fragment length polymorphism analysis of 16S-23S rDNA intergenic spacer region (IGS), and sequence analyses of IGS and housekeeping genes glnII and recA. Based on the rDNA IGS RFLP by means of three restriction enzymes rhizobia were grouped in five clusters (I-V). By sequence analysis of 16S-23S rDNA IGS identified genotypes of horsegram rhizobia were distributed into five divergent lineages of Bradyrhizobium genus which comprised (I) the IGS type IV rhizobia and valid species B. yuanmingense, (II) the strains of IGS type I and Bradyrhizobium sp. ORS 3257 isolated from Vigna sp., (III) the strains of the IGS type II and Bradyrhizobium sp. CIRADAc12 from Acacia sp., (IV) the IGS type V strains and Bradyrhizobium sp. genospecies IV, and (V) comprising genetically distinct IGS type III strains which probably represent an uncharacterized new genomic species. Nearly, 87% of indigenous horsegram isolates (IGS types I, II, III, and V) could not be related to any other species within the genus Bradyrhizobium. Phylogeny based on housekeeping glnII and recA genes confirmed those results found by the analysis of the IGS sequence. All the isolated rhizobia nodulated Macrotyloma sp. and Vigna spp., and only some of them formed nodules on Arachis hypogeae. The isolates within each IGS type varied in their ability to fix nitrogen. Selection for high symbiotic effective strains could reward horsegram production in poor soils of South India where this legume is largely cultivated. PMID:21188591

  6. The nucleolus Yun Wah Lam*, Laura Trinkle-

    E-print Network

    Trinkle-Mulcahy, Laura

    'Christmas tree' model rDNA transcription rRNA processing Ribosome assembly Fluorescence microscopy Yun Wah knobs rRNA rDNA Fibrillar centre (FC) RPA39 Granular component (GC) B23 Large ribosomal subunit prominent structure in a cell nucleus. It is the site of ribosomal RNA (rRNA) transcription, pre

  7. Tetraploid Italian ryegrass (Lolium multiflorum Lam.)

    Microsoft Academic Search

    F. Wit

    1958-01-01

    1.Some preliminary experiments are described with C2- and C3-generations of tetraploid Italian ryegrass. In all cases the tetraploids had a lower dry matter content than the diploids.2.A C3-generation produced by mass selection equalled or surpassed the diploid mother strains in dry matter yield in some small experiments on several soil types in the first year. The tetraploids, however, were less

  8. MonicaS.Lam StanfordUniversity

    E-print Network

    Pratt, Vaughan

    Play:InternetAccess,TV,Telephone Communication Computation Media Games #12;EmergingCompu@ngModels Rich legacy apps Locality of reference Offline legacy apps Locality of reference Offline execution Device independent Central management In your pocket@ons %ofworkersalteringsecuritysecngs Dilemma: How to empower individuals and provide security? #12;LaptopsToday SystemStack:HW,OS,apps

  9. Life Science Fall 2011 110901 Eric Lam (Imperial College, London)

    E-print Network

    Zhao, Yuxiao

    in Human Cancers" Host: Anders Rosén 110929 Mahvash Tavassoli "Active PKC signaling sensitizes cancer cells to killing by apoptin; therapeutic perspectives" Host: Marek Los, IGEN 111013 Hjalmar Brismar (KI, KTH

  10. Ecology and Management of Diffuse Knapweed (Centaurea diffusa Lam.)

    Microsoft Academic Search

    Jim Jacobs; Sharlene Sing

    A close relative of spotted knapweed in the Asteraceae taxonomic family, diffuse knapweed is typically biennial, reproducing exclusively by seed. It forms a rosette with a central crown and tap root in the juvenile stage and a single upright stem one to three feet (0.3 to 0.9 m) tall with numerous spreading branches at maturity. White (occasionally purple) flowers are

  11. Haskell-Join Presented by: Edmund S. L. Lam

    E-print Network

    Sulzmann, Martin

    -calculus) "Parallelism is going mainstream". What does this mean? Haskell-Join ­ p. #12;The Big Picture "Parallelism is going mainstream". Haskell-Join ­ p. #12;The Big Picture "Parallelism is going mainstream". Haskell-Join ­ p. #12;The Big Picture "Parallelism is going mainstream". Haskell-Join ­ p. #12;The Big Picture

  12. Clinical role of a fixed combination of standardized Berberis aristata and Silybum marianum extracts in diabetic and hypercholesterolemic patients intolerant to statins

    PubMed Central

    Di Pierro, Francesco; Bellone, Iaele; Rapacioli, Giuliana; Putignano, Pietro

    2015-01-01

    Background Statin intolerance is a medical condition often leading patients to nonadherence to the prescribed therapy or to a relevant reduction of the statin dosage. Both situations determine a totally or partially uncontrolled lipid profile, and these conditions unquestionably increase the risk of cardiovascular events. Methods We enrolled hypercholesterolemic, type 2 diabetic patients complaining of intolerance to statins. Some of them had reduced the statin dose ‘until the disappearance of symptoms’; others had opted for treatment with ezetimibe; and yet others were not undergoing any treatment at all. All patients of the three groups were then given a fixed combination of berberine and silymarin (Berberol®), known from previous papers to be able to control both lipidic and glycemic profiles. Results The tested product both as a single therapy and as add-on therapy to low-dose statin or to ezetimibe reduced triglycerides, low-density lipoprotein cholesterol, fasting blood glucose, and glycosylated hemoglobin in a significant manner without inducing toxicity conditions that might be somehow ascribed to a statin-intolerant condition. Conclusion Our study demonstrates that use of Berberol®, administered as a single or add-on therapy in statin-intolerant subjects affected by diabetes and hypercholesterolemia is a safe and effective tool capable of improving the patients’ lipidic and glycemic profiles. PMID:25678808

  13. Whitebrush Response to Tebuthiuron and Picloram Pellets.

    E-print Network

    Scifres, C.J.; Embry, D.L.; Mutz, J.L.

    1981-01-01

    . Yellow thistle * - - Callicarpa americana Berberis trifoliolata Lycium berlandieri Acacia ridigula Quercus marilandica Buchloe dactyloides Leuchophyllum fru tescens Ambrosia artemisiifolia Hilaria berlangeri Lantana horrida Crataegus mollis...

  14. New variety and nomenclatural changes in neotropical Gaultheria (ericaceae)

    Microsoft Academic Search

    James L. Luteyn

    1989-01-01

    Gaultheria sclerophylla var.hirsuta Luteyn from Ecuador is described, illustrated, and discussed, and the following new combinations are validated:Gaultheria buxifolia var.elassantha (A. C. Smith) Luteyn,G. buxifolia var.secunda (E. J. Remy) Luteyn,G. lanigera var. rufolanata (Sleumer) Luteyn,G. strigosa var.revoluta (A. C. Smith) Luteyn,G. eriophylla var.mucronata (E. J. Remy) Luteyn,G. alnifolia var.grata (A. C. Smith) Luteyn,G. serrata (Vell. Conc.) Sleumer ex Kinoshita-Gouvêa.

  15. Antimicrobial and Antioxidant Activities of Bauhinia racemosa Lam. and Chemical Content.

    PubMed

    Rashed, Khaled; Butnariu, Monica

    2014-01-01

    Methanol 70% extract of Bauhinia racemosa aerial parts was tested for antimicrobial activity against different bacterial and fungal strains and for antioxidant activity and also total content of polyphenols with phytochemical analysis of the extract was determined. The results have showed that the extract has a significant antimicrobial activity, it inhibited the growth of Bacillus subtilis and also it was highly active against Candida albicans suggesting that it can be used in the treatment of fungal infections. The extract has a good antioxidant activity, it has shown high values of the trolox equivalent antioxidant capacity and polyphenol content while it has shown a lower value of oxygen radical absorbance capacity. Phytochemical analysis has shown that it has interesting phytochemical bioconstituents, include flavonoids, tannins and others, and the deep phytochemical study results in the isolation of methyl gallate, gallic, kaempferol, quercetin, querection 3-O-?-rhamnoside, kaempferol 3-O-?-glucoside, myricetin-3-O-?- glucoside, querection-3-O-rutinoside (Rutin). All compounds were identified by different spectroscopic analyses (UV, (1)H-NMR, (13)C-NMR) and Mass Spectrometry (MS). PMID:25276210

  16. Aroma volatiles from two fruit varieties of jackfruit ( Artocarpus heterophyllus Lam.)

    Microsoft Academic Search

    José Guilherme S Maia; Eloisa Helena A Andrade; Maria das Graças B Zoghbi

    2004-01-01

    The aroma volatiles from two fruit varieties of jackfruit (Artocarpus heterophyllus) growing in the Amazon were obtained by simultaneous distillation-extraction and analysed by GC–MS. The major components identified in the aroma concentrate of “hard jackfruit” variety were isopentyl isovalerate (28.4%) and butyl isovalerate (25.6%). The aroma concentrate of “soft jackfruit” was dominated by isopentyl isovalerate (18.3%), butyl acetate (16.5%), ethyl

  17. Some Physicochemical Properties of Jackfruit (Artocarpus heterophyllus Lam) Seed Flour and Starch

    Microsoft Academic Search

    Vanna Tulyathan; Kanitha Tananuwong; Prapa Songjinda; Nongnuj Jaiboon

    2002-01-01

    Some physicochemical and rheological properties of jackfruit seed flour and starch, isolated from the flour were investigated. The flour had good capacities for water absorption (205%) and oil absorption (93%). Substitution of wheat flour with the seed flour, at the level of 5, 10 and 20% markedly reduced the gluten strength of the mixed dough. The Brabender amylogram (6% concentration,

  18. Chemical and flavour changes in jackfruit ( Artocarpus heterophyllus Lam.) cultivar J3 during ripening

    Microsoft Academic Search

    B. T. Ong; S. A. H Nazimah; A. Osman; S. Y. Quek; Y. Y. Voon; D. Mat Hashim; P. M. Chew; Y. W. Kong

    2006-01-01

    Chemical composition (titratable acidity, moisture content, crude fibre, colour, pH, soluble solids, sugars, organic acids) and flavour changes during ripening of jackfruit (Artocarpus heterophyllus L.) cultivar J3 were evaluated. Significant (p<0.05) changes in titratable acidity and colour was obtained, but no significant changes were found in moisture and crude fiber contents during ripening. Total soluble solids and total sugars increased

  19. Preventive effects of Moringa oleifera (Lam) on hyperlipidemia and hepatocyte ultrastructural changes in iron deficient rats.

    PubMed

    Ndong, Moussa; Uehara, Mariko; Katsumata, Shinichi; Sato, Shigeru; Suzuki, Kazuharu

    2007-08-01

    The effects of Moringa oleifera (MO), Moringaceae on hyperlipidemia and hepatocyte ultrastructural changes caused by iron deficiency were investigated. Four-week-old male Wistar-strain rats were fed a control diet based on AIN-93G (C), an iron deficient diet (FeD), a FeD + 0.5% MO (FeD-m) diet, or a FeD + MO 1% (FeD-M) diet for 4 weeks. It was found that MO reduced iron-deficient diet-induced increases in serum and hepatic lipids with dose-dependent increases of serum quercetin and kaempherol, but did not prevent anemia. By electron microscopy, in iron deficient hepatocytes, slightly swollen mitochondria and few glycogen granules were observed, but glycogen granules increased and mitochondria were normalized by treatment with MO. Furthermore, lipoproteins were observed in the Golgi complex under treatment with MO. These results suggest a possible beneficial effect of MO in the prevention of hyperlipidemia and ultrastructural changes in hepatocytes due to iron-deficiency. PMID:17690476

  20. A spatial light modulator for terahertz beams Wai Lam Chan,1

    E-print Network

    Mittleman, Daniel

    Engineering, Rice University, MS 366, Houston, Texas 77251-1892, USA 2 Los Alamos National Laboratory, Center speed. © 2009 American Institute of Physics. DOI: 10.1063/1.3147221 Recent advances in terahertz either require cryogenic cooling9 or have a poor modulation depth.10 Other previous efforts in building

  1. Creating, Destroying, and Restoring Value in Wikipedia Reid Priedhorsky, Jilin Chen, Shyong (Tony) K. Lam,

    E-print Network

    Priedhorsky, Reid

    .3 [Group and Organization Interfaces]: Computer- supported cooperative work, web-based interaction General. As of this writing, it contains nearly two million articles and ranks among the top ten most visited sites on the Web ­ there is no credential check- ing. Changes are visible to everyone immediately, without any review cycle. However

  2. Genome Sequence of the Sulfate-Reducing Bacterium Desulfotomaculum hydrothermale Lam5T

    E-print Network

    Boyer, Edmond

    and thermal ecosystems (1). Evi- dence of their presence in terrestrial geothermal hot springs has been a Tunisian hot spring. The genome is composed of 2.7 Mb, with a G C content of 49.48%, and it contains 2 draft genome sequence of a Desulfotomaculum spe- cies isolated from a terrestrial hot spring in Tunisia

  3. Physio-anatomic aspects on the initial growth of Guazuma ulmifolia Lam. seedlings.

    PubMed

    Scalon, Silvana P Q; Pereira, Heloisa H G; Glaeser, Daniele F; Silva, Jocemar J; Betoni, Roseli; Mussury, Rosilda M

    2011-06-01

    This paper aimed to evaluate the initial growth of "mutambo" seedlings in different conditions of light intensity and treatments with gibberellic acid (GA). The seedlings were kept under full sun and 50% of shading. Sixty days after the emergence, seedlings were sprayed with: 1) 100 mg L(-1) GA(3); 2) 200 mg L(-1) GA(3); 3) control. At the end of the appraisals, seedlings height under 50% of shading was compared to the height that were growing under full sun with 200 mg L(-1) GA. Stem diameter was lower under shading. Leaf area did not vary among the treatments, but the root system growth was higher under full sun and did not vary among GA levels. The number of stomata, trichomes and epidermal cells on adaxial and abaxial sides was higher under full sun. Total dry masses of leaf and root were highe runder full sun and with 200 mg L(-1) GA application. "Mutambo" seedlings presented a higher initial growth under full sun, although with a lower height, diameter, and lenght of the largest root and total dry masses of leaf and root were higher. A concentration with 200 mg L(-1) promoted a higher growth. PMID:21670888

  4. In Vitro and In Vivo Cytogenotoxic Effects of Hot Aqueous Extract of Achyrocline satureioides (Lam.) DC.

    PubMed Central

    Cariddi, L. N.; Sabini, M. C.; Escobar, F. M.; Bacchetti, R.; Montironi, I.; Merckis, C.; Reinoso, E. B.; Núñez Montoya, S.; Zanon, S. M.; Comini, L. R.; Sabini, L. I.

    2015-01-01

    In this work we extend the toxicological studies of hot aqueous extract of A. satureioides (As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extract in vivo. In addition, the extract was chemically characterized. Finally, we established a comparison with previous data of cold aqueous extract. The As-HAE induced cytotoxicity on PBMCs determined by trypan blue dye exclusion (CC50 = 653??g/mL) and MTT (CC50 = 588??g/mL) assays being more toxic than cold extract. However, As-HAE as well as cold extract did not induce apoptosis measured by Hoechst 33258 staining, TUNEL assay, and DNA fragmentation analysis. The in vivo micronucleus test showed that As-HAE exerted cytogenotoxic effects on bone marrow of mice, contrary to what was observed with cold extract. The chemical study of As-HAE allowed identifying the flavonoids found in cold extract: luteolin, quercetin, and 3-O-methylquercetin, but at higher concentrations. We suggest that toxic effects induced by As-HAE could be due to high concentrations of these flavonoids. Given that As-HAE is the most used in folkloric medicine, its administration should be controlled in order to prevent potential cell damage.

  5. Distribution of polycyclic aromatic hydrocarbons in subcellular root tissues of ryegrass (Lolium multiflorum Lam.)

    Microsoft Academic Search

    Fuxing Kang; Dongsheng Chen; Yanzheng Gao; Yi Zhang

    2010-01-01

    BACKGROUND: Because of the increasing quantity and high toxicity to humans of polycyclic aromatic hydrocarbons (PAHs) in the environment, several bioremediation mechanisms and protocols have been investigated to restore PAH-contaminated sites. The transport of organic contaminants among plant cells via tissues and their partition in roots, stalks, and leaves resulting from transpiration and lipid content have been extensively investigated. However,

  6. Ore Extensions and V -domains S. K. Jain, T. Y. Lam and A. Leroy

    E-print Network

    Paris-Sud XI, Université de

    a source of many interesting examples in noncommutative ring theory. In particular, differential polynomial ring over a "universal differential" field was the first exam- ple of a simple right-left V -domain differential polynomial rings or localizations of twisted polynomial rings (Cf. [C],[Os1],[Os2]). In this paper

  7. Ore Extensions and V -domains S. K. Jain, T. Y. Lam and A. Leroy

    E-print Network

    Jain, Surender K.

    a source of many interesting examples in noncommutative ring theory. In particular, differential polynomial ring over a "universal differential" field was the first exam- ple of a simple right-left V -domain differential polynomial rings or localizations of twisted polynomial rings (Cf. [C],[Os]). In this paper, we

  8. Identity and delimitation of the American species of Litsea Lam. (Lauraceae): a morphological approach

    Microsoft Academic Search

    Nelly del C. Jiménez-Pérez; Francisco G. Lorea-Hernández

    2009-01-01

    The genus Litsea in America is a small group of species with high variability that has not been evaluated rigorously; authors recognize either\\u000a three or as many as 11 species because of the unclear delimitation of taxa. Our focus in this study was to resolve the number,\\u000a names, and distinguishing features of the American species of Litsea, based on strict

  9. Identification, distribution, and control of triasulfuron-resistant Italian ryegrass (Lolium multiflorum Lam.) in Central Texas 

    E-print Network

    Tucker, Kevin Patrick

    2002-01-01

    et al. 1994). The first report of herbicide resistance was that of triazine resistant common groundsel (Senecio vulgaris L. ) in 1970 (Heap 1997; Holt and Lebaron 1990; Ryan 1970). Since that time, over 150 herbicide resistant weed species... vulgaris L. ) confers cross-resistance to other sulfonylureas, but little or no cross-rcsistancc to imidazolinones (Ewart et al. 1992). This is likely due to different point mutations on the ALS enzyme. A single point mutation in Arabidopsis confers...

  10. Low Profile Rigid UHF RFID Tags K. V. S. Rao, Sander F. Lam, Pavel V. Nikitin

    E-print Network

    Hochberg, Michael

    chamber using our National Instruments PXI RF LabView system which was described in [8]. Figure 4 presents, present simulation and measurement results, and discuss typical applications. I. INTRODUCTION Rigid simulation tool Ansoft HFSS which calculated tag antenna gain, impedance, matching to the RFID chip

  11. Effectiveness of lifesaving skills training and improving institutional emergency obstetric care readiness in Lam Dong, Vietnam.

    PubMed

    Sloan, Nancy L; Nguyen, Thi Nhu Ngoc; Do, Trong Hieu; Quimby, Charlotte; Winikoff, Beverly; Fassihian, Goli

    2005-01-01

    Essential obstetric care is promoted as the prime strategy to save women's lives in developing countries. We measured the effect of improving lifesaving skills (LSS) capacity in Vietnam, a country in which most women deliver in health facilities. A quasi-experimental study was implemented to assess the impact of LSS training and readiness (availability of essential obstetric equipment, supplies, and medication) on the diagnosis of life-threatening obstetric conditions and appropriate management of labor and birth. The intervention (LSS training and readiness) was provided to all clinics and hospitals from 1 of 3 demographically similar districts in southcentral Vietnam, to hospitals only in another district, with the third district serving as the comparison group. Detection of life-threatening obstetric conditions increased in both experimental clinics and hospitals, but the intervention only improved the management of these conditions in hospitals. Management of life-threatening obstetric conditions is most effective in hospitals. The intervention did not clearly benefit women delivering in clinics. PMID:15973269

  12. Benjamin Livshits and Monica S. Lam 1. PHPList Admin Page SQL Injection Vulnerability

    E-print Network

    Livshits, Ben

    Mac OS X Font Book Font Collection Buffer Overflow Vulnerability 24. OpenBook Admin.PHP SQL Injection Vulnerability 23. Apple Mac OS X Font Book Font Collection Buffer Overflow Vulnerability 24. OpenBook Admin

  13. Agrobacterium tumefaciens -mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.)

    Microsoft Academic Search

    A. J. E. Bettany; S. Dalton; E. Timms; B. Manderyck; M. Dhanoa; P. Morris

    2003-01-01

    Agrobacterium tumefaciens strain LBA4404 carrying plasmid pTOK233 encoding the hygromycin resistance (hph) and #-glucuronidase (uidA) genes has been used to transform two agronomic grass species: tall fescue (Festuca arundinacea) and Italian ryegrass (Lolium multiflorum). Embryogenic cell suspension colonies or young embryogenic calli were co-cultured with Agrobacterium in the presence of acetosyringone. Colonies were grown under hygromycin selection with cefotaxime and

  14. Feeding preferences of the collembolan Onychiurus sinensis for fungi colonizing holm oak litter (Quercus rotundifolia Lam.)

    E-print Network

    Paris-Sud XI, Université de

    . Two fungi (Penicillium spinulosum and the Basidiomycete S41) attracted Collembola by their odour of our test fungi, but not on the Basidiomycete S41 and on Trichoderma polysporum. Collembola / feeding alimentaires / litière / champignon / odeur de champignon 1. INTRODUCTION Collembola (Hexapoda) used

  15. The LAM\\/MPI Checkpoint\\/Restart Framework: System-Initiated Checkpointing

    Microsoft Academic Search

    Sriram Sankaran; Jeffrey M. Squyres; Brian Barrett; Andrew Lumsdaine; Jason Duell; Paul Hargrove; Eric Roman

    2003-01-01

    As high-performance clusters continue to grow in size and popularity, issues of fault tolerance and reliability are becoming limiting factors on application scalability. To ad- dress these issues, we present the design and implementa- tion of a system for providing coordinated checkpointing and rollback recovery for MPI-based parallel applications. Our approach integrates the Berkeley Lab BLCR kernel- level process checkpoint

  16. Antimicrobial, Antioxidant, and Wound Healing Properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC.

    PubMed Central

    Agyare, Christian; Dwobeng, Anita Serwaa; Agyepong, Nicholas; Boakye, Yaw Duah; Mensah, Kwesi Boadu; Ayande, Patrick George; Adarkwa-Yiadom, Martin

    2013-01-01

    Microbial infections of various types of wounds are a challenge to the treatment of wounds and wound healing. The study was to investigate antimicrobial and antioxidant properties of methanol leaf and stem bark extracts of Kigelia africana and methanol leaf and root extracts of Strophanthus hispidus and also to determine wound healing properties of the extracts. The antimicrobial activities of the methanol extracts were determined against two Gram-positive and two Gram-negative bacteria and a fungus using agar diffusion and micro-dilution methods. The antioxidant activity was determined using 1,1-diphenyl-2-picryl–hydrazyl (DPPH) method. The influence of the extracts on rate of wound closure was investigated using the excision wound model and histopathological investigation of treated and untreated wound tissues performed. The MICs of leaf extract of K. africana against test organisms were 2.5–7.5?mg/mL and stem bark extract were 2.25–7.5?mg/mL. The leaf extract of S. hispidus had MIC range of 2.5–7.5?mg/mL and 2.5–10?mg/mL for root extract. The IC50 of leaf and stem bark extracts of K. africana were 56.9 and 13.7??g/mL, respectively and leaf and root of S. hispidus were 49.8 and 45.1??g/mL, respectively. K. africana extracts (7.5% w/w) showed significant (P < 0.05) wound contraction at day 7 with 72% of wound closure whiles significant (P < 0.05) wound contractions were observed on day 11 for stem bark of K. africana, leaf and root extracts of S. hispidus. Wound tissues treated with the extracts showed improved collagenation, re-epitheliazition and rapid granulation formation compared with untreated wound tissues. The extracts were found to contain alkaloids, saponins, tannins, flavonoids, carbohydrates, and sapogenetic glycosides. The HPLC finger-printing of the extracts were developed. The leaf, stem bark and root extracts of K. africana and S. hispidus exhibited antimicrobial, antioxidant, and enhanced wound healing properties and these may justify the medicinal uses of the plants for treatment of microbial infections and wounds. PMID:23662099

  17. On the heats of formation of formyl cyanide and thioformyl cyanide Thanh Lam Nguyen

    E-print Network

    Nguyen, Minh Tho

    parameters for both formyl and thioformyl cyanides using ab initio molecular orbital theory.7 Geometrical for the sulfur ana- logue Hf,298 0 thioformyl cyanide 222 30 kJ/mol. In view of the large discrepancy and also

  18. Seeding rate and seed size as management techniques for ryegrass (Lolium Multiflorum, Lam) in winter wheat 

    E-print Network

    Cook, Casey Lee

    2005-08-29

    Higher seeding rates and larger seed sizes could enhance the competitiveness of wheat with ryegrass. Growth room and field research evaluated the effects of wheat seeding rates and seed size in competition with Italian ryegrass. Winter wheat seeds...

  19. Triterpenes and Steroids from Euphorbia denticulata Lam. With Anti-Herpes Symplex Virus Activity

    PubMed Central

    Shamsabadipour, Sara; Ghanadian, Mustafa; Saeedi, Hojjatollah; Rahimnejad, Mohammad Reza; Mohammadi-Kamalabadi, Marzieh; Ayatollahi, Seyed Majid; Salimzadeh, Loghman

    2013-01-01

    In this research, dried acetone:chloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3?,25-diol, cycloart-23E-ene-3?,25- diol, ergosta-8,24-dien-3-ol (obtusifoliol) and beta-sitosterol which were reported for the first time from this plant. The structure of these compounds was elucidated by NMR and mass spectroscopic methods. The MTS assay was used to determine the toxicity and antiviral activity of betulin and (3?,23E)-cycloarta-23-ene-3,25-diol. Betulin showed anti-HSV-1 activity with EC50 value of 84.37±0.02 ?g/mL, and toxicity on normal vero cells with CC50 value of 660.718±0.072 ?g/mL. (3?,23E)-Cycloarta-23-ene-3,25-diol showed antiviral effect with EC50 value of 86.63±0.03 ?g/mL, and toxicity with CC50 value of 1089.21±0.25 ?g/mL. The results revealed that these two compounds have the antiviral activity far below the CC50 value with selectivity index (CC50/EC50) values of 7.83, and 12.57, respectively. PMID:24523756

  20. Homage to Manjusri (Byang-chub-lam-gyi-rim-pahi-nams-len) (trans.)

    E-print Network

    Lhalungpa, Lobsang

    1968-02-29

    1 l2! ~ .ci! ~ Ji! (l]~ ~1.Jli1 ~ ~ lJl __ --9 Jil J1 .L.: -B 1 ,--9i, Nni ~) ai' Ii ..J! ~ (zn2!~ ~ ~~ -B Ji ht lJ. '-'! .,; .2! ,,: • ~ .....9l ....,....-""' (:3 \\l:3 32 ~ ~! )..J vu1 21 n 11 .E . Jl') 2:! n~ ~ , ,.A"'.,' OL.I-<..i J1...

  1. Gastroprotective effect of aqueous extract and mucilage from Bryophyllum pinnatum (Lam.) Kurz

    PubMed Central

    Sharma, Abhishek L.; Bhot, Meeta A.; Chandra, Naresh

    2014-01-01

    Context: Bryophyllum pinnatum is used as traditional medicine in India, Africa, Tropical America and China for treatment of various diseases. B. pinnatum contains different groups of phytoconstituents viz., flavonoid, terpenoids, alkaloid, phenolic compounds. Aim: The present study was carried out to evaluate the gastroprotective activity of B. pinnatum whole plant aqueous extract, and mucilage (MUC) isolated from the whole plant against ethanol induced gastric ulcer. Materials and Methods: Pretreatment of rats with aqueous extract at dose level of 500 and 750 mg/kg b.w., MUC at 500 mg/kg dose level and standard drug Rabeprazole at dose level of 20 mg/kg b.w. where given for 7 days. Results: The aqueous whole plant extract of B. pinnatum at dose of 750 mg/kg p.o. and MUC at dose of 500 mg/kg p.o. markedly decrease the incidence of ulcers in ethanol induced ulcer rats. In ethanol induced ulcer rats, there was a decrease in the gastric volume, free and total acidity and ulcerative index as compared to the control group. Total carbohydrate content was found to be an increase as compare to control the group. The aqueous whole plant extract of B. pinnatum at dose of 750 mg/kg showed a significant reduction in the above parameters which was comparable to the standard drug rabeprazole (20 mg/kg). B. pinnatum extract and MUC showed protection index 72.69 and 69.65% respectively, whereas standard drug rabeprazole showed protection index 75.49%. Conclusions: Whole plant extracts of B. pinnatum and MUC has potent gastroprotective effect which can be further clinically studied for new drug development. PMID:25593406

  2. Establishment of bermudagrass (Cynodon dactylon (L.) Pers.) seeded with annual ryegrass (Lolium multiflorum Lam

    E-print Network

    Bowmer, William Jackson

    1967-01-01

    seedings. However, some herbicides now available offer promise as tools to aid in grass establishment. Lee (1965) and Wicks et al. (1965) used 3-amino-t, Z, 4-triazole (amitrole), Z-methoxy-3, 6-dichlorobenzoic acid (dicamba), 2, 4-bis (iso... that annual ryegrass inhibited respiration and thus growth of seedlings of tobacco, timothy and barley. Root cells darkened and became necrotic. Welbank (1963) found that ryegrass contained about the same quantities of toxin per unit weight of dry matter...

  3. High genetic differentiation among remnant populations of the endangered Caesalpinia echinata Lam. (Leguminosae-Caesalpinioideae)

    Microsoft Academic Search

    M. A. CARDOSO; J. PROVAN; W. POWELL; P. C. G. FERREIRA; D. E. DE OLIVEIRA

    1998-01-01

    Forest fragments along the Atlantic coastland of Brazil have been highly impacted by extensive human activities for the last 400 years. Caesalpinia echinata (Leguminosae- Caesalpinioideae), brazilwood, was overexploited during this period due to its economi- cal importance as a dye. As a result, the species has become endangered and today its total population size is very restricted. We have assessed

  4. Algebraic Solutions of the Lam e Equation, Revisited Robert S. Maier 1

    E-print Network

    Maier, Robert S.

    by separation of variables. In that context, its solutions in­ clude the ellipsoidal harmonics. In classical arose in a classical setting: the solution of Laplace's equation in ellipsoidal coordinates

  5. Copper-catalyzed Chan-Lam coupling between sulfonyl azides and boronic acids at room temperature.

    PubMed

    Moon, Soo-Yeon; Nam, Jungsoo; Rathwell, Kris; Kim, Won-Suk

    2014-01-17

    A mild and efficient method for the synthesis of N-arylsulfonamides in the presence of 10 mol % of CuCl is demonstrated. The reaction proceeds readily at room temperature in an open flask using a variety of sulfonyl azides and boronic acids without any base, ligand, or additive. PMID:24404934

  6. Vital technology as a human right By Arthur Lerner-Lam, Leonardo Seeber, and Robert Chen

    E-print Network

    Qian, Ning

    after one disaster. The region of the Indian Ocean hit by the tsunami is afflicted by typhoons year quantified the exposures of people and their economies to multiple natural hazards. For the most part, we, making the next steps obvious: · Map known exposures of populations and economic activities to multiple

  7. Discovery of an allosteric site in the caspases Jeanne A. Hardy, Joni Lam, Jack T. Nguyen

    E-print Network

    Hardy, Jeanne

    an important class of drug targets for stroke, ischemia, cancer, and inflammatory diseases (2). The active-like conformation. This approach may be useful to identify new allosteric sites from natural or engineered cysteines activation (Fig. 1A), enabling them to cleave downstream targets, which ultimately results in cell death

  8. Amelioration of ionizing radiation induced lipid peroxidation in mouse liver by Moringa oleifera Lam. leaf extract.

    PubMed

    Sinha, Mahuya; Das, Dipesh Kr; Datta, Sanjukta; Ghosh, Santinath; Dey, Sanjit

    2012-03-01

    Protective effect of Moringa oleifera leaf extract (MoLE) against radiation-induced lipid peroxidation has been investigated. Swiss albino mice, selected from an inbred colony, were administered with MoLE (300 mg/kg body wt) for 15 days before exposing to a single dose of 5 Gy 60Co-gamma radiation. After treatments, animals were necropsied at different post irradiation intervals (days 1, 7 and 15) and hepatic lipid peroxidation and reduced glutathione (GSH) contents were estimated to observe the relative changes due to irradiation and its possible amelioration by MoLE. It was observed that, MoLE treatment restored GSH in liver and prevented radiation induced augmentation in hepatic lipid peroxidation. Phytochemical analysis showed that MoLE possess various phytochemicals such as ascorbic acid, phenolics (catechin, epicatechin, ferulic acid, ellagic acid, myricetin) etc., which may play the key role in prevention of hepatic lipid peroxidation by scavenging radiation induced free radicals. PMID:22439436

  9. Immunomodulatory effects of alcbholic and hydroalcoholic extracts of Moringa olifera Lam leaves.

    PubMed

    Banji, Otilia J F; Banji, David; Kavitha, R

    2012-04-01

    Effects of 50, 100 and 200 mg/kg body weight of the alcoholic and hydro-alcoholic extract of leaves of M. olifera were studied on various immune paradigms like delayed type hypersensitivity reaction using SRBC as an antigen, determination of antibody titer, neutrophil adhesion test as an indicator for neutrophil index, total leucocyte count in cyclophosphamide induced immunosuppressed animals and carbon clearance assay as a measure of phagocytic activity. Hydro-alcoholic extract of M. olifera substantially enhanced cellular immune response, humoral immune response, neutrophil index and phagoctic activity in doses of 100 and 200 mg/kg body weight. The ethanolic extract (200 mg/kg body weight) was efficient in improving immune response. The results suggest that M. olifera has a significant role to play as an immune stimulator. PMID:22611915

  10. Silicon Photonicsfor Optical Access Networks Ryohei Urata, Hong Liu, Cedric Lam, Pedram Dashti, and Chris Johnson

    E-print Network

    Tomkins, Andrew

    ]. In this paper, we discuss the potential role of silicon photonics for realizing key device technologies which@google.com Abstract: We highlight promising developments and directions in silicon photonics for realizing cost effective WDM-PON: photonic integration for integrated WDM transceivers at the OLT and widely tunable laser

  11. (Analysis of MAssive Data in Earth and Universe Sciences) CeSAM -LAM

    E-print Network

    Canet, Léonie

    Functional Dependencies · Focus on visualisation · Use of TULIP software · Focus on usage of skyline approach;Visualisation with TULIP Objectives : provide visual representations to the experts in order to check

  12. Citrus tristeza virus replicates and forms infectious virions in protoplasts of resistant citrus relatives

    Microsoft Academic Search

    Maria R. Albiach-Marti; Jude W. Grosser; Siddarame Gowda; Munir Mawassi; Tatineni Satyanarayana; Stephen M. Garnsey; William O. Dawson

    2004-01-01

    Citrus tristeza virus (CTV) is the most economically important viral disease of citrus worldwide. Cultivars with improved CTV tolerance or resistance are needed to manage CTV-induced diseases. The citrus relatives Poncirus trifoliata (L.) Raf., Swinglea glutinosa (Blanco) Merr., and Severinia buxifolia (Poir) Ten. are potential sources of CTV resistance, but their resistance mechanisms are poorly characterized. As a first step

  13. UHF RFID TAG CHARACTERIZATION: OVERVIEW AND STATE-OF-THE-ART Pavel Nikitin, KVS Rao, Sander Lam

    E-print Network

    Hochberg, Michael

    frequency identification (RFID) based on modulated backscatter is a wireless technology with a long history wireless systems where active transceivers communicate with each other. RFID transceiver (reader) transmits power link budget diagram of a monostatic RFID system, where the tag is located at a distance d away

  14. Effect of age, length, defoliation and storage of scion sticks on success of softwood grafting in jackfruit ( Artocarpus heterophyllus Lam)

    Microsoft Academic Search

    S A Desai; A G Desai

    1989-01-01

    The softwood grafting in jackfruit is a simple and rapid method of vegetative propagation. A success varying from 33·33–80%\\u000a has been reported in this method. Matured scion sticks of 6 months old recorded significantly highest sprouting (73·33%) and\\u000a survival (68·33%) of the grafts. With the increase in the length of scion sticks from 2·5–10cm, the percentage of success\\u000a also increased

  15. Aplicaciones de los Estándares Educativos a los Sistemas Virtua- les de Formación Colaborativos: Especificaciones para la Herra- mienta LAMS

    Microsoft Academic Search

    Luisa María Romero Moreno

    Resumen Dado el incremento de los campus virtuales en la mayoría de Universidades e Instituciones, así como del uso de eLearning en el ámbito de las empresas, se hace necesario un esfuerzo de planificación para que el uso de las TIC's aporte todo su potencial a los sistemas virtuales de forma- ción. Para ello los llamados estándares de tecnología edu-

  16. “ Erussica ”, the intergeneric fertile somatic hybrid developed through protoplast fusion between Eruca sativa Lam. and Brassica juncea (L.) Czern

    Microsoft Academic Search

    S. R. Sikdar; G. Chatterjee; S. Das; S. K. Sen

    1990-01-01

    Hypocotyl calli-derived protoplasts of two cultivars of Brassica juncea (2n=36), a major oil-seed crop, were fused with normal as well as ?-irradiated mesophyll protoplasts of Eruca sativa (2n=22). The irradiation of the Eruca fusion partner increased the plating efficiency as well as the morphogenic potentiality of the fusion products over the normal fusion. Fertile plants could be regenerated from such

  17. Antiplatelet and antithrombotic activities of essential oil from wild Ocotea quixos (Lam.) Kosterm. (Lauraceae) calices from Amazonian Ecuador.

    PubMed

    Ballabeni, Vigilio; Tognolini, Massimiliano; Bertoni, Simona; Bruni, Renato; Guerrini, Alessandra; Rueda, Gabriela Moreno; Barocelli, Elisabetta

    2007-01-01

    Ocotea quixos essential oil was shown to possess significant inhibitory activity of platelet aggregation and clot retraction in rodent plasma. This study is aimed at fully characterizing the antiplatelet activity of the whole essential oil and its main components trans-cinnamaldehyde and methyl cinnamate also in human plasma, at investigating the mechanism underlying such activity and at evaluating the potential antithrombotic activity of subacute treatment of mice with Ocotea essential oil. In vitro Ocotea essential oil and trans-cinnamaldehyde inhibited arachidonic acid-, U46619-, ADP-, phorbol12-myristate13-alcetate-, collagen-induced platelet aggregation and thrombin-induced clot retraction in human and rodent plasma; Ocotea oil and trans-cinnamaldehyde competitively antagonized contractions induced by thromboxane A2 receptor agonist U46619 in rat isolated aortic ring (K(B) = 18 and 3.2 microg ml(-1), respectively). In vivo Ocotea oil, orally administered in a subacute treatment (30-100 mg kg(-1) day(-1) for 5 days) to mice, prevented acute thrombosis induced by collagen-epinephrine intravenous injection. This antithrombotic activity was not accompanied by pro-haemorragic side effect, as detected by the inactivity in bleeding test, thus showing a favourable safety profile compared to the conventional antiplatelet agent, acetylsalicylic acid. Present findings indicate that Ocotea essential oil possesses potent and safe antithrombotic activity attributable to its antiplatelet and vasorelaxant effects. The main constituent trans-cinnamaldehyde seems to be the primary responsible for this activity through a putative mechanism involving the inhibition of thromboxane A2 receptors. PMID:17079160

  18. Juice of Bryophyllum pinnatum (Lam.) inhibits oxytocin-induced increase of the intracellular calcium concentration in human myometrial cells.

    PubMed

    Simões-Wüst, A P; Grãos, M; Duarte, C B; Brenneisen, R; Hamburger, M; Mennet, M; Ramos, M H; Schnelle, M; Wächter, R; Worel, A M; von Mandach, U

    2010-10-01

    The use of preparations from Bryophyllum pinnatum in tocolysis is supported by both clinical (retrospective comparative studies) and experimental (using uterus strips) evidence. We studied here the effect of B. pinnatum juice on the response of cultured human myometrial cells to stimulation by oxytocin, a hormone known to be involved in the control of uterine contractions by increasing the intracellular free calcium concentration ([Ca2+]i). In this work, [Ca2+]i was measured online during stimulation of human myometrial cells (hTERT-C3 and M11) with oxytocin, which had been pre-incubated in the absence or in the presence of B. pinnatum juice. Since no functional voltage-gated Ca2+ channels could be detected in these myometrial cells, the effect of B. pinnatum juice was as well studied in SH-SY5Y neuroblastoma cells, which are known to have such channels and can be depolarised with KCl. B. pinnatum juice prevented the oxytocin-induced increase in [Ca2+]i in hTERT-C3 human myometrial cells in a dose-dependent manner, achieving a ca. 80% inhibition at a 2% concentration. Comparable results were obtained with M11 human primary myometrial cells. In hTERT-C3 cells, prevention of the oxytocin-induced increase in [Ca2+]i was independent of the extracellular Ca2+ concentration and of voltage-dependent Ca2+-channels. B. pinnatum juice delayed, but did not prevent the depolarization-induced increase in [Ca2+]i in SH-SY5Y cells. Taken together, the data suggest a specific and concentration-dependent effect of B. pinnatum juice on the oxytocin signalling pathway, which seems to corroborate its use in tocolysis. Such a specific mechanism would explain the rare and minor side-effects in tocolysis with B. pinnatum as well as its high therapeutic index. PMID:20381326

  19. Antinocieptive and anti-inflammatory effects of Toddalia asiatica (L) Lam. (Rutaceae) root extract in Swiss albino mice

    PubMed Central

    Kariuki, Hellen Nyambura; Kanui, Titus Ikusya; Yenesew, Abiy; Patel, Nilesh; Mbugua, Paul Mungai

    2013-01-01

    Introduction Toddalia asiatica is a commonly used medicinal plant in East Africa for the management of pain and inflammatory conditions. The present study investigated the antinociceptive and the anti-inflammatory effects of T. asiatica in Swiss albino mice. Methods The antinociceptive and the anti-inflammatory effects of T. asiatica were investigated using formalin-induced pain test and the carrageenin-induced oedema paw. The extract solvent (vehicle), aspirin and indomethacin were employed as negative and positive controls respectively. Eight mice were used in each experiment. Results In the early phase of the formalin test, the 100mg/kg dose showed no significant antinociceptive activity while the 200mg/kg showed significant (p < 0.01) antinociceptive activity. The 100 mg/kg dose showed highly significant antinociceptive activity (p < 0.001) in the late phase of the formalin test while the 200mg/kg dose showed no significant antinociceptive activity. A reduction in carragenin induced acute inflammation paw oedema was significant (p < 0.01) following administration of 100mg/kg dose but not with the 200mg/kg dose. Conclusion The present study therefore lends support to the anecdotal evidence for use of T. asiatica in the management of painful and inflammatory conditions. PMID:23734278

  20. Cytoprotection by Achyrocline satureioides (Lam) D.C. and some of its main flavonoids against oxidative stress.

    PubMed

    Arredondo, M F; Blasina, F; Echeverry, C; Morquio, A; Ferreira, M; Abin-Carriquiry, J A; Lafon, L; Dajas, F

    2004-03-01

    Epidemiological studies indicate that dietary antioxidants can influence the incidence of neurodegenerative diseases. Among them flavonoids have been proposed to be effective cytoprotectors. Consequently, herbs with a high concentration of these compounds such as Achyrocline satureioides, Ginkgo biloba and Epilobium parviflorum are of special interest. In this context a comparative study of the cytoprotective capacity of infusions from the three plants against an oxidative insult was performed. Hence, the cytoprotective activity of each infusion against H2O2 injury to PC12 cells was tested and the antioxidant capacity was assessed by the ABTS*+ radical bleaching assay. Free and glycosylated flavonoids contained in the infusions were identified by HPLC and the cytoprotective effect of some of these individual flavonoids was tested. The analysis of the flavonoid content of the infusions revealed different profiles. Epilobium parviflorum infusion showed the highest antioxidant capacity but only Achyrocline satureioides infusion proved to be cytoprotective. Moreover, the free flavonoids quercetin and luteolin contained in this infusion were also cytoprotective. In conclusion, the free radical scavenger capacity did not correlate with the cytoprotective profile of the infusions. The special mixture of unglycosylated Achyrocline satureioides flavonoids could be a clue to explain the unique effect of this plant. PMID:15036461

  1. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam

    NASA Astrophysics Data System (ADS)

    Rathi Sre, P. R.; Reka, M.; Poovazhagi, R.; Arul Kumar, M.; Murugesan, K.

    2015-01-01

    Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines.

  2. Sweet potato [Ipomoea batatas (L.) Lam.] cultivated as tuber or leafy vegetable supplier as affected by elevated tropospheric ozone.

    PubMed

    Keutgen, Norbert; Keutgen, Anna J; Janssens, Marc J J

    2008-08-13

    Sweet potato cultivars respond differently to elevated tropospheric ozone concentrations of ca. 130 mug m (-3), 8 h a day for 4 weeks, which affects their selection for cultivation. In the first cultivar presented here, an adequate leafy vegetable supplier, the ozone load resulted in a shift of biomass to maintain the canopy at the expense of tuber development. Starch content of leaves was reduced, indicating an impairment of quality, but carotenoid content remained stable. The second cultivar may be grown for tuber production. Although the ratio tuber/plant remained stable under ozone, tuber yield and its starch content were significantly reduced. The lower starch content indicated a worse quality for certain industrial processing, but it is desirable for chip production. Elevated tropospheric ozone concentrations also influenced free amino acids and macronutrient contents of tubers, but these modifications were of minor significance for tuber quality in the second cultivar. PMID:18593180

  3. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam.

    PubMed

    Rathi Sre, P R; Reka, M; Poovazhagi, R; Arul Kumar, M; Murugesan, K

    2015-01-25

    Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines. PMID:25189525

  4. Seven different glucose-6-phosphate dehydrogenase variants including a new variant distributed in Lam Dong Province in southern Vietnam.

    PubMed

    Matsuoka, Hiroyuki; Thuan, Dang Thi Vinh; van Thien, Huynh; Kanbe, Toshio; Jalloh, Amadu; Hirai, Makoto; Arai, Meiji; Dung, Nguyen The; Kawamoto, Fumihiko

    2007-08-01

    We conducted a survey for glucose-6-phosphate dehydrogenase (G6PD) deficiency using blood samples from male outpatients of a local hospital in southern Vietnam. Most of the samples were from the Kinh (88.9%), the largest ethnic group in Vietnam, with a small number (11.1%) coming from the K'Ho, Chauma, Nung, and Tay minorities. We detected 25 G6PD-deficient cases among 1,104 samples (2.3%), and read the open reading frame of G6PD. A novel mutation (352T>C) predicting an aminoacid change of 118Tyr>His was found in a 1-year-old Kinh boy. His G6PD activity was estimated to be less than 10% residual activity, although he did not show chronic hemolytic anemia. Thus, we categorized this variant as Class II and named it G6PD Bao Loc. In the Kinh population, G6PD Viangchan (871G>A, 1311C>T, intron 11 nt93T>C), one of the most common variants in continental Southeast Asian populations, was the highest (6/19), followed by variants originating from the Chinese such as G6PD Canton (1376G>T) (5/19), G6PD Kaiping (1388G>A) (3/19), G6PD Gaohe (95A>G) (1/19), and G6PD Quing Yuan (392G>T) (1/19). In addition, G6PD Union (1360C>T) (2/19), which originated from the Oceania, was also detected. These findings suggest that the Kinh people are derived from various ancestries from continental Southeast Asia, China, and Oceania. In contrast, all of the 5 deficient cases in the K'Ho population were G6PD Viangchan, suggesting that they were very close to Southeast Asian populations such as the Khmer in Cambodia and the Lao in Laos. It is interesting that G6PD Mahidol (487G>A), another common variant in continental Southeast Asian populations in Myanmar, Thailand, and Malaysia, has not been detected from the Vietnamese. PMID:17726510

  5. Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass ( Lolium multiflorum Lam.)

    Microsoft Academic Search

    Bruno Studer; Beat Boller; Doris Herrmann; Eva Bauer; Ulrich K. Posselt; Franco Widmer; Roland Kölliker

    2006-01-01

    Bacterial wilt caused by Xanthomonas translucens pv. graminis (Xtg) is a major disease of economically important forage crops such as ryegrasses and fescues. Targeted breeding based on seedling inoculation has resulted in cultivars with considerable levels of resistance. However, the mechanisms of inheritance of resistance are poorly understood and further breeding progress is difficult to obtain. This study aimed to

  6. Asymmetric somatic hybridization between tall fescue ( Festuca arundinacea Schreb.) and irradiated Italian ryegrass ( Lolium multiflorum Lam.) protoplasts

    Microsoft Academic Search

    G. Spangenberg; M. P. Vallés; Z. Y. Wang; P. Montavon; J. Nagel; I. Potrykus

    1994-01-01

    Intergeneric asymmetric somatic hybrids have been obtained by the fusion of metabolically inactivated protoplasts from embryogenic suspension cultures ofFestuca arundinacea (recipient) and protoplasts from a non-morphogenic cell suspension ofLolium multiflorum (donor) irradiated with 10, 25, 50, 100, 250 and 500 Gy of X-rays. Regenerating calli led to the recovery of genotypically and phenotypically different asymmetric somatic hybridFestulolium plants. The genome

  7. Consistent detection of QTLs for crown rust resistance in Italian ryegrass ( Lolium multiflorum Lam.) across environments and phenotyping methods

    Microsoft Academic Search

    Bruno Studer; Beat Boller; Eva Bauer; Ulrich K. Posselt; Franco Widmer; Roland Kölliker

    2007-01-01

    Crown rust, caused by Puccinia coronata f. sp. lolii, is one of the most important diseases of temperate forage grasses, such as ryegrasses (Lolium spp.), affecting yield and nutritional quality. Therefore, resistance to crown rust is a major goal in ryegrass breeding\\u000a programmes. In a two-way pseudo-testcross population consisting of 306 Lolium multiflorum individuals, multisite field evaluations as well as

  8. Effects of olive mill wastewater physico-chemical treatments on polyphenol abatement and Italian ryegrass (Lolium multiflorum Lam.) germinability.

    PubMed

    Barbera, A C; Maucieri, C; Ioppolo, A; Milani, M; Cavallaro, V

    2014-04-01

    Direct spreading on agricultural lands may represent an environmentally friendly disposal method and a possible use of water and nutrients from olive mill wastewaters (OMWs). However, the agronomic use of OMWs is limited, among others by polyphenols, which exert phytotoxic effects. Activated charcoal (AC) has been recognized as a very effective agent for polyphenol abatement, as it enables an irreversible process of phenol adsorption. Addition of calcium hydroxide (Ca(OH)2) has also been described as a cheap and effective method in polyphenols abatement. However, the effects of Ca(OH)2 addition to OMW on seed germination are unclear. In this paper, the effects of AC and/or Ca(OH)2 on OMW polyphenols abatement, and Lolium multiflorum seed germination have been investigated. The highest polyphenols removal, approximately 95%, was observed when 80 g L(-1) of AC was added to OMWs (the maximum dose in this investigation). The addition of Ca(OH)2 not only improved the effectiveness of the AC treatment but also resulted in a significant rise in Lolium seed germination at the highest AC doses (60 and 80 g L(-1)). Considering the high salinity (7300 ?S cm(-1)) of these wastewaters, low quantities of Ca(OH)2 may also exert a protective effect on soil structure counteracting the sodium-induced dispersion through the binding action of calcium cation on clays and organic matter. PMID:24289894

  9. Bioactivity of Piper guineense Schum. & Thonn seed and Moringa oleifera Lam. leaf powder against Trogoderma granarium Everts (Coleoptera: Dermestidae)

    Microsoft Academic Search

    Samuel Adelani Babarinde; Olufemi O. Richard Pitan; Adekunle Tosin Ogunfiade

    2011-01-01

    Laboratory studies were carried out to investigate bioactivity of Piper guineense seeds and Moringa oleifera leaf powders applied singly or in a mixture against larvae and adult Trogoderma granarium Everts in airtight containers. Three levels (0.0 g, 0.5 g, and 1.0 g\\/20 g groundnut seeds) of the plant powders were used and pirimiphos-methyl was applied at 0.01 g\\/20 g seeds (recommended dose). Another control consisting of

  10. A synthetic approach to N-aryl carbamates via copper-catalyzed Chan-Lam coupling at room temperature.

    PubMed

    Moon, Soo-Yeon; Kim, U Bin; Sung, Dan-Bi; Kim, Won-Suk

    2015-02-01

    A mild and efficient synthesis of N-arylcarbamates was achieved by reacting azidoformates with boronic acids in the presence of 10 mol % of copper chloride catalyst. The reaction proceeds readily in an open flask at room temperature without additional base, ligand, or additive. Rapid access to urea analogues via a two-step one-pot procedure is enabled by reacting N-arylcarbamates with aluminum-amine complexes. In addition, among several boronic acid derivatives prepared, dimethylphenyl boronate was found to react rapidly in its reaction with benzyl azidoformate, invoking in situ generation of this species in the catalytic cycle. PMID:25584697

  11. Evolution de l'indice foliaire d'un peuplement de ray-grass d'Italie (Lolium multiflorum Lam.)

    E-print Network

    Paris-Sud XI, Université de

    mesure où ce paramè- tre conditionne l'interception du rayonnement solaire et l'activité photosynthétique productivité (P) d'un couvert végétal est en rela- tion avec le rayonnement intercepté par ses organes densité de flux de rayonnement utile à la photosynthèse, absorbée par la plante (« Photosyn- thetically

  12. Plant regeneration of Iris pallida Lam. and Iris germanica L. via somatic embryogenesis from leaves, apices and young flowers.

    PubMed

    Jéhan, H; Courtois, D; Ehret, C; Lerch, K; Pétiard, V

    1994-09-01

    Irones are violet-scented ketonic compounds contained in the rhizome of certain species of iris. As cultivation of the iris tends to decrease, a selection program has been initiated to find the best performing clones in terms of growth and yield. Parallel to this selection, in vitro regeneration studies have been carried out in order to multiply interesting clones. A method of rapid multiplication by somatic embryogenesis associated with multibudding was developed. Callus was obtained from leaf bases, flower pieces or rhizome apices; the best explants were flower pieces. The induction media used to obtain embryogenic callus were Murashige & Skoog (1962) media. Assays with adding of proline in these media have showed that it could double the yield of embryogenic callus. The embryogenic expression medium was the Knudson's orchid agar (Knudson 1946) medium. Conformity of the plants obtained was checked by comparing their chemotypes with those of the mother plants. PMID:24193517

  13. Evaluation of the cytotoxicity, genotoxicity and apoptotic induction of an aqueous extract of Achyrocline satureioides (Lam.) DC.

    PubMed

    Sabini, M C; Cariddi, L N; Escobar, F M; Mañas, F; Comini, L; Reinoso, E; Sutil, S B; Acosta, A C; Núñez Montoya, S; Contigiani, M S; Zanon, S M; Sabini, L I

    2013-10-01

    Achyrocline satureioides is widely consumed as infusion or aperitif and shows important therapeutic properties. Previously, we reported absence of genotoxicity of cold aqueous extract (CAE) of A. satureioides by Allium test. However, one test cannot predict the genotoxic effects of a substance. Thus, the aim of this work was to investigate cytotoxicity, genotoxicity and apoptotic ability of CAE of A. satureioides. In addition, CAE was chemically characterized. The cytotoxicity was evaluated by Trypan blue and MTT assays. The apoptotic capacity was evaluated by Hoechst staining and DNA fragmentation-analysis. The genotoxicity was studied by comet assay (CA) and micronucleus test. The identification and quantification of flavonoids were performed by HPLC-ESI-MS/MS. The cytotoxicity studies indicated low toxicity of CAE. In addition, CAE did not induce apoptotic effects on human PBMCs. CAE did not show genotoxicity in vitro against Vero cells, at 10-50 ?g/mL. CAE did not induce in vivo genotoxic effects, but it showed at high concentrations cytotoxicity by micronucleus assay. CAE presented flavonoids such as quercetin, 3-O-methylquercetin and luteolin. In conclusion, A. satureioides at popularly concentrations used, in aperitif or infusion, can be consumed safely because did not show any cytotoxic or genotoxic effects. PMID:23948353

  14. Effect of heat–moisture treatment on digestibility of different cultivars of sweet potato (Ipomea batatas (L.) Lam) starch

    PubMed Central

    Senanayake, Suraji; Gunaratne, Anil; Ranaweera, K K D S; Bamunuarachchi, Arthur

    2014-01-01

    Different heat–moisture levels were applied to native starches from different cultivars of sweet potatoes available in Sri Lanka (Wariyapola red, Wariyapola white, Pallepola variety, Malaysian variety and CARI 273) to study the digestibility level. Samples were treated with 20, 25, and 30% moisture at 85°C and 120°C for 6 h and in vitro starch digestibility was tested with porcine pancreatin enzyme. A range of 19.3–23.5% digestibility was shown by the native starches with no significant difference (P < 0.05). Significant changes were observed in the digestibility level of the hydrothermally modified starches and the moisture content showed a positive impact on the digestibility. Heat–moisture treatment at 85°C brought an overall increase in digestibility and temperature beyond 85°C had a negative impact. No significant difference (P < 0.05) in the digestibility was observed with 20% and 25% moisture at 85°C and increased level were seen at 85°C and 30% moisture. PMID:25473497

  15. Compile-Time Optimizations for Tensor Contraction Expressions Gerald Baumgartner, Daniel Cociorva, Chi-Chung Lam, P. Sadayappan

    E-print Network

    Baumgartner, Gerald

    developed in the context of a program syn- thesis system for a class of quantum chemistry computa- tions- initio quantum chemistry models, including models that are too tedious for manual development by quantum are express- ible as a set of tensor contractions. Currently, manual de- velopment of accurate quantum

  16. On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity

    PubMed Central

    Roullier, C; Kambouo, R; Paofa, J; McKey, D; Lebot, V

    2013-01-01

    New Guinea is considered the most important secondary centre of diversity for sweet potato (Ipomoea batatas). We analysed nuclear and chloroplast genetic diversity of 417 New Guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical America. The molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical America. Nuclear data confirm previous results, suggesting that New Guinea landraces are principally derived from the Northern neotropical genepool (Camote and Batata lines, from the Caribbean and Central America). However, chloroplast data suggest that South American clones (early Kumara line clones or, more probably, later reintroductions) were also introduced into New Guinea and then recombined with existing genotypes. The frequency distribution of pairwise distances between New Guinea landraces suggests that sexual reproduction, rather than somaclonal variation, has played a predominant role in the diversification of sweet potato. The frequent incorporation of plants issued from true seed by farmers, and the geographical and cultural barriers constraining crop diffusion in this topographically and linguistically heterogeneous island, has led to the accumulation of an impressive number of variants. As the diversification of sweet potato in New Guinea is primarily the result of farmers' management of the reproductive biology of their crop, we argue that on-farm conservation programmes that implement distribution of core samples (clones representing the useful diversity of the species) and promote on-farm selection of locally adapted variants may allow local communities to fashion relatively autonomous strategies for coping with ongoing global change. PMID:23531982

  17. Physiological and biochemical changes associated with flower development and senescence in so far unexplored Helleborus orientalis Lam. cv. Olympicus.

    PubMed

    Shahri, Waseem; Tahir, Inayatullah; Islam, Sheikh Tajamul; Bhat, Mushtaq Ahmad

    2011-03-01

    The so far unexplored H. Orientalis cv. Olympicus exhibits a unique pattern of flower senescence, involving re-greening of creamy white petaloid sepals at the later stages. The greenish sepals become photosynthetically competent immediately after pollination and persist until the seeds are set. After the seed set, the entire (green) flower abscises from the plant. Flower development of Helleborus orientalis cv. Olympicus growing in the open was divided into six stages (I-VI) from tight bud stage to the senescent stage. The average life span of an individual flower after it is fully open is about 6 days. Membrane permeability of sepal tissues estimated as electrical conductivity of leachates increased during senescence. The content of sugars and soluble proteins in the sepal tissues increased during flower opening and declined thereafter during senescence. The protease activity increased as the flower progressed towards senescence. From the present study, it becomes evident that decline in the sugar status and elevation in specific protease activity leading to degradation of proteins are the important factors regulating development and senescence in H. orientalis flowers. Although the tissue content of soluble proteins registered an overall quantitative decrease but SDS-PAGE of protein extract from sepal tissues suggested a decrease in the expression of high molecular weight proteins and an increase in low molecular weight proteins during flower development and senescence. At this stage it is not known whether the polypeptides that increased during senescence play an important role in the senescence of Helleborus orientalis flowers. The increase in these polypeptides during flower senescence is of particular interest because they may be linked to flower longevity. Understanding the nature of these proteins can provide new insights into the pathways that execute senescence and the post-transcriptional regulation of senescence in this flower system. PMID:23572993

  18. Exchange-Correlation Energy Density from Virial Theorem Federico G. Cruz, Kin-Chung Lam, and Kieron Burke*

    E-print Network

    Burke, Kieron

    be approximated as a functional of the spin densities. The local spin density (LSD) approximation Exc LSD has long of exact exchange with GGA exchange-correlation. Typical bond energy errors are about 30 kcal/mol in LSD, 8, some have spurious extrema, and overall, look worse than their LSD counterparts. So how do they produce

  19. PVAc TUTKALI KULLANILARAK YAPI?TIRILMI? LAM?NE AH?AP YAPI ELAMANLARININ YANMA ÖZELL?KLER?

    Microsoft Academic Search

    Ramazan ÖZEN; Burhanettin UYSAL; Z. K. Ü. Safranbolu; Meslek Yüksekokulu

    In this study, the combustion properties of three ply laminated wood material has been searched. In the produce of laminated wood material, scotch pine (Pinus sylvestris L.) has been used for faces ply. Lime-tree (Morus alba L.), white mulberry (Tillia perfifolia Ehrh), white oak (Quercus petraea spp.) and Uluda? fir (Abies bornmülleriana Mattf.) have been used for core ply of

  20. Spatial pattern and uncertainty of soil carbon and nitrogen in a subtropical savanna landscape in southern Texas 

    E-print Network

    Liu, Feng

    2009-05-15

    : Zanthoxylum fagara, Celtis pallida, Condalia hookeri, Diospyros texana, Schaefferia cunefolia, Ziziphus obtusifolia and Berberis trifoliolata. The grassland matrix between woody patches is dominated by C 4 grasses such as Paspalum setaceum, Setaria...

  1. DYNAMIC INTERMEDIATE MODELS FOR AUDIOGRAPHIC SYNTHESIS Vincent Goudard Hugues Genevois milien Ghomi Boris Doval

    E-print Network

    Paris-Sud XI, Université de

    Ghomi Boris Doval LAM - Institut Jean Le Rond d'Alembert vincent@mazirkat.o rg LAM - Institut Jean LeDYNAMIC INTERMEDIATE MODELS FOR AUDIOGRAPHIC SYNTHESIS Vincent Goudard Hugues Genevois Émilien Rond d'Alembert genevois@lam.jussi eu.fr LRI ­ Université Paris Sud 11 emilien.ghomi@lri.fr LAM

  2. Crystal structure of the human laminin receptor precursor.

    PubMed

    Jamieson, Kelly V; Wu, Jinhua; Hubbard, Stevan R; Meruelo, Daniel

    2008-02-01

    The human laminin receptor (LamR) interacts with many ligands, including laminin, prions, Sindbis virus, and the polyphenol (-)-epigallocatechin-3-gallate (EGCG), and has been implicated in a number of diseases. LamR is overexpressed on tumor cells, and targeting LamR elicits anti-cancer effects. Here, we report the crystal structure of human LamR, which provides insights into its function and should facilitate the design of novel therapeutics targeting LamR. PMID:18063583

  3. The effects of medium composition and culture conditions on in vitro rooting and ex vitro establishment of jackfruit ( Artocarpus heterophyllus Lam.)

    Microsoft Academic Search

    M. A. Rahman; Jennet Blake

    1988-01-01

    A sucrose level of 20 g\\/l in the culture medium and a 12 h photoperiod were required for optimal in vitro rooting of proliferated jackfruit shoots and these culture conditions also promoted subsequent establishment of plantlets in the glasshouse. High agar levels of 10 and 20 g\\/l reduced the humidity in culture but also reduced in vitro rooting and had

  4. Preparation and evaluation of antioxidant capacity of Jackfruit ( Artocarpus heterophyllus Lam.) wine and its protective role against radiation induced DNA damage

    Microsoft Academic Search

    Umesh B. Jagtap; Shailesh R. Waghmare; Vinayak H. Lokhande; Penna Suprasanna; Vishwas A. Bapat

    2011-01-01

    Jackfruit is an underutilized edible fruit in the tropics and subtropics. The aim of this study was to produce wine from jackfruit pulp and to evaluate the total phenolic and flavonoid contents and antioxidant properties of the wine. The ability of scavenging free radicals was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant assay (FRAP), N, N-dimethyl-p-phenylendiamine (DMPD) and nitric oxide

  5. The dominance of P-wave reflectivity over S-wave reflectivity from crustal fault zones suggests that variations in Lam param-

    E-print Network

    of dealing with only P-P scattering in imaging crustal re- flectors. In fact, the mode conversion problem coefficients, making it pos- sible to devise an effective imaging technique to image VP and signatures here. For instance, Aki (1992) shows that P-S conversion for earth- quake sources is much greater than

  6. Chromosome pairing of individual genomes in tall fescue (Festuca arundinacea Schreb.), its progenitors, and hybrids with Italian ryegrass (Lolium multiflorum Lam.).

    PubMed

    Kopecký, D; Bartos, J; Zwierzykowski, Z; Dolezel, J

    2009-01-01

    A diploid-like pairing system prevents meiotic irregularities and improves the efficiency of gamete production in allopolyploid species. While the nature of the system is known in some polyploid crops including wheat, little is known about the control of chromosome pairing in polyploid fescues (Festuca spp.). In this work we studied chromosome pairing in allohexaploid F. arundinacea, its progenitors F. pratensis and F. glaucescens, and two intergeneric hybrids Lolium multiflorum (2x) x F. arundinacea (6x) and L. multiflorum (4x) x F. glaucescens (4x). The use of genomic in situ hybridization (GISH) permitted the analysis of homoeologous chromosome pairing and recombination of different genomes involved. We detected a diploid-like pairing system in polyploid fescues F. arundinacea and F. glaucescens, the latter being one of the progenitors of F. arundinacea. The pairing control system was absent in the second progenitor F. pratensis. Detailed analysis of intergeneric hybrids confirmed the presumed haploinsufficiency of the fescue system, which resulted in homoeologous pairing between all component genomes. This indicates that introgression of any specific chromosome segment from one genome to another is possible in all genome combinations. Our results not only contribute to the quest to discover the nature of the system controlling chromosome pairing in polyploid fescues, but may also have serious implications for design of hybrid breeding schemes in forage grasses. PMID:19420930

  7. Composition and allelopathic effect of essential oils of two thistles: Cirsium creticum (Lam.) D.'Urv. ssp. triumfetti (Lacaita) Werner and Carduus nutans L

    Microsoft Academic Search

    Carmen Formisano; Daniela Rigano; Felice Senatore; Vincenzo De Feo; Maurizio Bruno; Sergio Rosselli

    2007-01-01

    Cirsium and Carduus are two of the main genera of true thistles. Different species in these genera share a quantity of secondary metabolites and have interesting ecological properties. The essential oils of two species, Cirsium creticum and Carduus nutans, were analysed, showing the presence as main compounds of 4-ethyl guaiacol (15%), hexadecanoic acid (10.6%), (E)-?-damascenone (7.8%), dihydroactinidiolide (6.0%) and 4-vinyl

  8. Productivity of alley farming with leucaena ( Leucaena leucocephala Lam. de Wit) and Napier grass ( Pennisetum purpureum K. Schum) in coastal lowland Kenya

    Microsoft Academic Search

    J. G. Mureithi; R. S. Tayler; W. Thorpe

    1995-01-01

    Inadequate supply of fodder is a serious constraint to the potentially-promising small-holder-dairy production system in coastal Kenya. Alley farming could be an approach to addressing this problem. A study of forage production based on Napier grass and leucaena in an alley cropping system was conducted on an infertile sandy soil in lowland coastal Kenya. The effects of leucaena hedgerows,Clitoria ternatea

  9. Transportation and Traffic Theory 2009 Edited by W.H.K. Lam, S.C. Wong and H.K. Lo

    E-print Network

    Levinson, David M.

    ' behavior enough to reduce congestion by charging them for the externalities they create. This paper aims describes the modeling of recurring congestion in a network. It is shown that the standard economic models of marginal cost cannot describe precisely traffic congestion in networks during time-dependent conditions

  10. Approximate String Matching using Compressed Suffix Trinh N.D. Huynh 1 , WingKai Hon 2 , TakWah Lam 2## , and WingKin Sung 1# # #

    E-print Network

    Lam, Tak-Wah

    Approximate String Matching using Compressed Suffix Arrays # Trinh N.D. Huynh 1 , Wing­Kai Hon 2 and gives O(|A| k m k+2 + occ) query time. Our solution also allows us to exploit compressed suffix arrays is very long, people would like to preprocess it to accelerate pattern queries. In the literature

  11. Self-Reseeding Potential and Herbage Production of Italian Ryegrass (Lolium Multiflorum Lam.) Affected by Date and Intensity of Initial Harvest

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Annually-sown cool-season small grain cereals can provide a valuable source of cool-season feeding for livestock in the southern Great Plains of the USA, but for many farmers limited access to field equipment for cultivation and planting is an obstacle to their use. Italian ryegrass (Lolium multiflo...

  12. Microbial growth and nitrogen immobilization in the root zone of barley ( Hordeum vulgare L.), Italian ryegrass ( Lolium multiflorum Lam.), and white clover ( Trifolium repens L.)

    Microsoft Academic Search

    T. A. Breland; L. R. Bakken

    1991-01-01

    The effect of roots on microbial growth and N immobilization was investigated in a pot experiment with barley, Italian ryegrass, and white clover. We used a silty subsoil with a low soil organic matter content (0.16%C and 0.012%N), which allowed us to measure N immobilization as an increase in total soil organic N (planted versus unplanted). At sampling, the soil

  13. Development of simple sequence repeat (SSR) markers and construction of an SSR-based linkage map in Italian ryegrass ( Lolium multiflorum Lam.)

    Microsoft Academic Search

    Mariko Hirata; Hongwei Cai; Maiko Inoue; Nana Yuyama; Yuichi Miura; Toshinori Komatsu; Tadashi Takamizo; Masahiro Fujimori

    2006-01-01

    In order to develop simple sequence repeat (SSR) markers in Italian ryegrass, we constructed a genomic library enriched for (CA)n-containing SSR repeats. A total of 1,544 clones were sequenced, of which 1,044 (67.6%) contained SSR motifs, and 395 unique clones were chosen for primer design. Three hundred and fifty-seven of these clones amplified products of the expected size in both

  14. Overexpression of horsegram (Macrotyloma uniflorum Lam.Verdc.) NAC transcriptional factor (MuNAC4) in groundnut confers enhanced drought tolerance.

    PubMed

    Pandurangaiah, Merum; Lokanadha Rao, G; Sudhakarbabu, O; Nareshkumar, A; Kiranmai, K; Lokesh, U; Thapa, Ganesh; Sudhakar, Chinta

    2014-08-01

    The NAC family being the largest plant-specific transcription factors functions in diverse and vital physiological processes during development. NAC proteins are known to be crucial in imparting tolerance to plants against abiotic stresses, such as drought and salinity, but the functions of most of them are still elusive. In this study, we report for the first time expression of the MuNAC4, a member of NAC transcription factor from horsegram (Macrotyloma uniflorum) conferring drought tolerance. The groundnut (Arachis hypogaea) transgenics were generated using recombinant MuNAC4 binary vector transformation approach. Molecular analysis of these transgenic lines confirmed the stable gene integration and expression of the MuNAC4 gene. Twelve lines of T5 generation exhibited significantly enhanced tolerance to drought stress with proliferated lateral root growth as compared to wild types. Transgenics exposed to long-term desiccation stress assays showed increased lateral roots and greenish growth. The physiological parameters analysis also suggests that overexpression of MuNAC4 plays a significant role in improving the water stress tolerance of transgenic groundnut, reducing the damage to membrane structures and enhancing osmotic adjustment and antioxidative enzyme regulation under stress. This study validates MuNAC4 as an important candidate gene for future phytoengineering approaches for drought tolerance in crop plants. PMID:24748414

  15. In vitro antimicrobial activity of the leaf extract of Harungana madagascariensis Lam. Ex Poir. (Hypericaceae) against strains causing otitis externa in dogs and cats.

    PubMed

    Moulari, B; Pellequer, Y; Chaumont, J P; Guillaume, Y C; Millet, J

    2007-03-01

    Otitis externa in dogs and cats is always caused by a combination of yeasts and bacteria, among which the most important are Malassezia pachydermatis, Staphylococcus intermedius and Pseudomonas species. These organisms often develop resistance to classical antimicrobial agents. Therefore, the aim of this study was to investigate the antimicrobial activities of an ethyl acetate leaf extract of Harungana madagascariensis against the organisms cited, to carry out the phytochemical investigation of this extract and to determine its bioactive chemical class using dilution techniques, the bioautography method and the standard phytochemical method described by Harborne (1973). Phytochemical analysis revealed the presence of saponins, tannins, flavonoids, alkaloids and anthracenic derivatives. The bioassay showed that the antimicrobial properties may be attributed to astilbin, a flavanone derivative identified on the basis of its spectroscopic data. The results suggest that the extract could be used in an antimicrobial preparation effective against the whole range of organisms incriminated in otitis externa in dogs and cats, with a minimal inhibitory concentration (MIC) of 250 microg/ml. PMID:17385560

  16. Herbicidal activity of Brassicaceae seed meal on wild oat (Avena fatua), Italian ryegrass (Lolium multiflorum Lam.), redroot pigweed (Amaranthus retroflexus L.) and prickly lettuce (Lactuca serriola)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There is an on-going need for the development of sustainable methods of weed control in crop production systems. Studies were conducted to evaluate the efficacy of different Brassicaceae seed meals and application rates on the emergence of several weed species including wild oat, Italian rye grass, ...

  17. Using the Internet in Middle Schools: A Model for Success. A Collaborative Effort between Los Alamos National Laboratory (LANL) and Los Alamos Middle School (LAMS).

    ERIC Educational Resources Information Center

    Addessio, Barbara K.; And Others

    Los Alamos National Laboratory (LANL) developed a model for school networking using Los Alamos Middle School as a testbed. The project was a collaborative effort between the school and the laboratory. The school secured administrative funding for hardware and software; and LANL provided the network architecture, installation, consulting, and…

  18. Using a Caesalpinia echinata Lam. protease inhibitor as a tool for studying the roles of neutrophil elastase, cathepsin G and proteinase 3 in pulmonary edema.

    PubMed

    Cruz-Silva, Ilana; Neuhof, Christiane; Gozzo, Andrezza Justino; Nunes, Viviane Abreu; Hirata, Izaura Yoshico; Sampaio, Misako Uemura; Figueiredo-Ribeiro, Rita de Cássia; Neuhof, Heinz; Araújo, Mariana da Silva

    2013-12-01

    Acute lung injury (ALI) is characterized by neutrophil infiltration and the release of proteases, mainly elastase (NE), cathepsin G (Cat G) and proteinase 3 (PR3), which can be controlled by specific endogenous inhibitors. However, inhibitors of these proteases have been isolated from different sources, including plants. For this study, CeEI, or Caesalpinia echinata elastase inhibitor, was purified from C. echinata (Brazil-wood) seeds after acetone fractionation, followed by ion exchange and reversed phase chromatographic steps. Characterization with SDS-PAGE, stability assays, amino acid sequencing and alignment with other protein sequences confirmed that CeEI is a member of the soybean Kunitz trypsin inhibitor family. Like other members of this family, CeEI is a 20 kDa monomeric protein; it is stable within a large pH and temperature range, with four cysteine residues forming two disulfide bridges, conserved amino acid residues and leucine-isoleucine residues in the reactive site. CeEI was able to inhibit NE and Cat G at a nanomolar range (with K(i)s of 1.9 and 3.6 nM, respectively) and inhibited PR3 within a micromolar range (K(i) 3.7 ?M), leading to hydrolysis of specific synthetic substrates. In a lung edema model, CeEI reduced the lung weight and pulmonary artery pressure until 180 min after the injection of zymosan-activated polymorphonuclear neutrophils. In experiments performed in the presence of a Cat G and PR3, but not an NE inhibitor, lung edema was reduced only until 150 min and pulmonary artery pressure was similar to that of the control. These results confirm that NE action is crucial to edema establishment and progression. Additionally, CeEI appears to be a useful tool for studying the physiology of pulmonary edema and provides a template for molecular engineering and drug design for ALI therapy. PMID:24140156

  19. Evaluation of anti-urolithiatic effect of aqueous extract of Bryophyllum pinnatum (Lam.) leaves using ethylene glycol-induced renal calculi

    PubMed Central

    Shukla, Apexa Bhanuprasad; Mandavia, Divyesh Rasikbhai; Barvaliya, Manish Jasmatbhai; Baxi, Seema Natvarlal; Tripathi, Chandrabhanu Rajkishore

    2014-01-01

    Materials and Methods : Thirty-six Wistar male rats were randomly divided into six equal groups. Group A animals received distilled water for 28 days. Group B to group F animals received 1% v/v ethylene glycol in distilled water for 28 days and group B served as ethylene glycol control. Groups C and D (preventive groups) received aqueous extract of leaves of B. pinnatum 50 and 100 mg/kg intraperitoneally, respectively for 28 days. Groups E and F (treatment groups) received aqueous extract of leaves of B. pinnatum 50 and 100 mg/kg intraperitoneally, respectively from 15th to 28th day. On days 0 and 28, 24 hrs urine samples were collected for urinary volume and urinary oxalate measurement. On day 28, blood was collected for serum creatinine and blood urea level monitoring. All animals were sacrificed and kidneys were removed, weighed, and histopathologically evaluated for calcium oxalate crystals deposition. Results: Administration of aqueous extract of leaves of B. pinnatum reduced urine oxalate level ?significantly, as compared with Group B (p<0.001). Serum creatinine and blood urea level were ?improved significantly in all aqueous extract of leaves of B. pinnatum-treated groups. Relative ?kidney weight and calcium oxalate depositions were found significantly reduced in animals ?received ABP as compared with Group B (p<0.001). ? Conclusions: B. pinnatum is effective in prevention and treatment of ethylene glycol-induced urolithiasis. PMID:25050313

  20. Energy E#cient Online Deadline Scheduling HoLeung Chan # WunTat Chan + TakWah Lam # LapKei Lee # KinSum Mak #

    E-print Network

    Wong, Prudence W.H.

    ­e#cient scheduling focuses on minimizing the energy to complete all jobs on a processor with unbounded speed, giving usage. 1 Introduction Deadline scheduling. Let us first review a classical on­ line problem of deadline of the jobs completed by their deadlines (see, e.g., [4,7,12]). # Department of Computer Science, University

  1. Antioxidant activity of Artocarpus heterophyllus Lam. (Jack Fruit) leaf extracts: remarkable attenuations of hyperglycemia and hyperlipidemia in streptozotocin-diabetic rats.

    PubMed

    Omar, Haidy S; El-Beshbishy, Hesham A; Moussa, Ziad; Taha, Kamilia F; Singab, Abdel Nasser B

    2011-01-01

    The present study examines the antioxidative, hypoglycemic, and hypolipidemic activities of Artocarpus heterophyllus (jack fruit) leaf extracts (JFEs). The 70% ethanol (JFEE), n-butanol (JFBE), water (JFWE), chloroform (JFCE), and ethyl acetate (JFEAE) extracts were obtained. Both JFEE and JFBE markedly scavenge diphenylpicrylhydrazyl radical and chelate Fe+2 in vitro. A compound was isolated from JFBE and identified using 1D and 2D 1H- and 13C-NMR. The administration of JFEE or JFBE to streptozotocin (STZ)-diabetic rats significantly reduced fasting blood glucose (FBG) from 200 to 56 and 79 mg%, respectively; elevated insulin from 10.8 to 19.5 and 15.1 µU/ml, respectively; decreased lipid peroxides from 7.3 to 5.4 and 5.9 nmol/ml, respectively; decreased %glycosylated hemoglobin A1C (%HbA1C) from 6.8 to 4.5 and 5.0%, respectively; and increased total protein content from 2.5 to 6.3 and 5.7 mg%, respectively. Triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), VLDL-C, and LDL/HDL ratio significantly declined by -37, -19, -23, -37, and -39%, respectively, in the case of JFEE; and by -31, -14, -17, -31, and -25%, respectively, in the case of JFBE; as compared to diabetic rats. HDL-C increased by +37% (JFEE) and by +11% (JFBE). Both JFEE and JFBE have shown appreciable results in decreasing FBG, lipid peroxides, %HbA1C, TC, LDL-C, and TG levels, and increasing insulin, HDL-C, and protein content. The spectrometric analysis confirmed that the flavonoid isolated from JFBE was isoquercitrin. We can conclude from this study that JFEE and JFBE exert hypoglycemic and hypolipidemic effects in STZ-diabetic rats through an antioxidative pathway that might be referred to their flavonoid contents. PMID:21479350

  2. DCEG Award Recipients - Anand Pathak, MD, PhD, MPH, Olusegun Onabajo, PhD, Clara Lam, PhD, MPH

    Cancer.gov

    To understand the role of IFN-?4 during HCV infection, we performed detailed functional analysis of IFN-?4. We suggest that a pathogenic effect of IFN-?4 expression in human hepatic cells is associated with inhibition of cell proliferation causing increased cell death and induction of pro-inflammatory cytokines. Chronic induction of IFN-?4 in HCV-infected hepatocytes leading to cell death and tissue repair may result in hepatic fibrosis and cirrhosis, which are relevant factors affecting clearance of HCV and, possibly progression to hepatocellular carcinoma.

  3. Moringa oleifera Lam. leaf extract prevents early liver injury and restores antioxidant status in mice fed with high-fat diet.

    PubMed

    Das, Nilanjan; Sikder, Kunal; Ghosh, Santinath; Fromenty, Bernard; Dey, Sanjit

    2012-06-01

    Consumption of high-fat diet (HFD) induces nonalcoholic fatty liver disease (NAFLD) and may lead to multiple complications affecting human health. In the present study, effect of Moringa oleifera leaf extract (MoLE) in alleviating HFD induced liver injury in mice has been reported. Liver histology and serum activity of hepatic marker enzymes i.e. aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) have been studied. Lipid peroxidation (LPO), ferric reducing antioxidant power (FRAP) and reduced glutathione (GSH) were also estimated using liver homogenate. Results of the study suggested that MoLE treatment protected HFD-induced liver damage as indicated by histopathology and liver enzyme activity compared to only-HFD fed group (P < 0.05). Interestingly, early signs of HFD-induced fatty liver were also alleviated by MoLE. Moreover, significant increase in endogenous antioxidant parameters and lower lipid peroxidation were found in liver of all MoLE treated groups. Results of the study indicated that MoLE has both preventive as also curative hepatoprotective activity. PMID:22734251

  4. African eggplant (Solanum anguivi Lam.) fruit with bioactive polyphenolic compounds exerts in vitro antioxidant properties and inhibits Ca2+-induced mitochondrial swelling

    PubMed Central

    Elekofehinti, Olusola Olalekan; Kamdem, Jean Paul; Bolingon, Aline Augusti; Athayde, Margareth Linde; Lopes, Seeger Rodrigo; Waczuk, Emily Pansera; Kade, Ige Joseph; Adanlawo, Isaac Gbadura; Rocha, Joao Batista Teixeira

    2013-01-01

    Objective To evaluate the antioxidant and radical scavenging activities of Solanum anguivi fruit (SAG) and its possible effect on mitochondrial permeability transition pore as well as mitochondrial membrane potential (??m) isolated from rat liver. Methods Antioxidant activity of SAG was assayed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power, iron chelation and ability to inhibit lipid peroxidation in both liver and brain homogenate of rats. Also, the effect of SAG on mitochondrial membrane potential and mitochondrial swelling were determined. Identification and quantification of bioactive polyphenolics was done by HPLC-DAD. Results SAG exhibited potent and concentration dependent free radical-scavenging activity (IC50/DPPH=275.03±7.8 µg/mL). Reductive and iron chelation abilities also increase with increase in SAG concentration. SAG also inhibited peroxidation of cerebral and hepatic lipids subjected to iron oxidative assault. SAG protected against Ca2+ (110 µmol/L)-induced mitochondrial swelling and maintained the ??m. HPLC analysis revealed the presence of gallic acid [(17.54±0.04) mg/g], chlorogenic acid (21.90±0.02 mg/g), caffeic acid (16.64±0.01 mg/g), rutin [(14.71±0.03) mg/g] and quercetin [(7.39±0.05) mg/g]. Conclusions These effects could be attributed to the bioactive polyphenolic compounds present in the extract. Our results suggest that SAG extract is a potential source of natural antioxidants that may be used not only in pharmaceutical and food industry but also in the treatment of diseases associated with oxidative stress. PMID:24075339

  5. Production of 4-ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of Ipomoea batatas Lam.: a comparative study.

    PubMed

    Mohanraj, Remya; Subha, S

    2015-05-01

    A comparative study was done on the production of 4-ipomeanol from root tubers of Ipomoea batatas and its rhizogenic callus. Best callusing response was obtained on MS medium supplemented with 11 ?M NAA (?-Naphthaleneacetic acid) and 1 ?M KIN (Kinetin). Effect of various elicitors (Fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. Methanol extract of the samples were purified by column chromatography and detected using TLC. Identification of 4-ipomeanol was confirmed using HPLC and quantified spectrophotometrically. A mass spectrum was recorded to confirm the presence of 4-ipomeanol. The calli grown under chitin produced highest (6.61mg g(-1)) amount of 4-ipomeanol. This is the first report on in vitro production of 4-ipomeanol from I. batatas. Since 4-ipomeanol is reported to be present only in I. batatas, this study would help in standardizing protocols for large scale production without affecting its natural flora. PMID:26040027

  6. Parsing dimensional versus diagnostic category-related patterns of reward circuitry function in behaviorally and emotionally dysregulated youth in the Longitudinal Assessment of Manic Symptoms (LAMS) study

    PubMed Central

    Bebko, Genna; Bertocci, Michele A.; Fournier, Jay C.; Hinze, Amanda K.; Bonar, Lisa; Almeida, Jorge R. C.; Perlman, Susan B.; Versace, Amelia; Schirda, Claudiu; Travis, Michael; Gill, Mary Kay; Demeter, Christine; Diwadkar, Vaibhav A.; Ciuffetelli, Gary; Rodriguez, Eric; Olino, Thomas; Forbes, Erika; Sunshine, Jeffrey L.; Holland, Scott K; Kowatch, Robert. A.; Birmaher, Boris; Axelson, David; Horwitz, Sarah M.; Arnold, L. Eugene; Fristad, Mary. A; Youngstrom, Eric A.; Findling, Robert L.; Phillips, Mary L.

    2014-01-01

    Context Pediatric disorders characterized by behavioral and emotional dysregulation pose diagnostic and treatment challenges because of high comorbidity, suggesting that they may be better conceptualized dimensionally rather than categorically. Identifying neuroimaging measures associated with behavioral and emotional dysregulation in youth may inform understanding of underlying dimensional vs. disorder-specific pathophysiology. Objective Identify, in a large cohort of behaviorally and emotionally dysregulated youth, neuroimaging measures that: 1) are associated with behavioral and emotional dysregulation pathological dimensions (behavioral and emotional dysregulation measured with the Parent General Behavior Inventory 10 Item Mania Scale [PGBI-10M], mania, depression, anxiety); or 2) differentiate diagnostic categories(BPSD, ADHD, anxiety, disruptive behavior disorders (DBD)). Design Multi-site neuroimaging study(February 2011–April 2012). Setting Academic medical centers: Case Western Reserve University, Cincinnati Children’s Hospital, University of Pittsburgh. Patients Referred sample of behaviorally and emotionally dysregulated youth(n=85) from the Longitudinal Assessment of Manic Symptoms study and healthy youth (n=20). Main Outcome Measures Region-of-interest analyses examined relationships among prefrontal-ventral striatal reward circuitry during a reward paradigm (Win, Loss, control conditions), symptom dimensions, and diagnostic categories. Results Regardless of diagnosis, higher PGBI-10M scores were associated with greater left middle prefrontal cortical (mPFC; r=0.28), and greater levels of anxiety with greater right dorsal anterior cingulate cortical (dACC; r=0.27), activity to Win. The 20 highest (t=2.75) and 20 lowest (t=2.42) PGBI-10M scoring youth showed significantly greater left mPFC activity to Win than 20 healthy youth. DBD were associated with lower left ventrolateral prefrontal cortex(VLPFC) activity to Win (t=2.68) (all ps<0.05, corrected). Conclusions Greater PGBI-10M-related left mPFC activity, and greater anxiety-related right dACC activity, to Win may reflect heightened reward sensitivity and greater attention to reward in behaviorally and emotionally dysregulated youth, regardless of diagnosis. Reduced left VLPFC activity to Win may reflect reward insensitivity in youth with DBD. Despite a distinct reward-related neurophysiology in DBD, findings generally support a dimensional approach to studying neural mechanisms in behaviorally and emotionally dysregulated youth. PMID:24285346

  7. Status of the South American tuberous legume Pachyrhizus tuberosus (Lam.) Spreng.: Field observations, taxonomic analysis, linguistic studies and agronomic data on the diversity of the South American Pachyrhizus tuberosus (Lam.) Spreng. complex with special reference to the identification of two new cultivar groups from Ecuador and Peru

    Microsoft Academic Search

    MARTEN SØRENSEN; SØREN DØYGAARD; JAIME E. ESTRELLA; LARS PETER KVIST; POUL ERIK NIELSEN

    1997-01-01

    The tuberous legume Pachyrhizus tuberosus is found in cultivation sporadically throughout the tropical lowlands of South America.\\u000a As a result of field studies conducted in Bolivia, Ecuador, Peru and Venezuela, three distinct cultivar groups have been identified.\\u000a Rare references presenting further evidence in support of this grouping, and yielding information on the cultivation history,\\u000a are reviewed. The differences in morphology,

  8. Evaluation of a large-scale invasive plant species herbicide control program in the Berkshire Taconic Plateau, Massachusetts, USA

    Microsoft Academic Search

    Lindsay McAlpine; Stephen Porder

    SUMMARY In the late 1990's, The Nature Conservancy (TNC) documented a rise in five invasive plant species, barberry Berberis thunbergii , bittersweet Celastrus orbiculatus , garlic mustard Alliaria petiolata , buckthorn Frangula alnus , and honeysuckle Lonicera morrowii on the periphery of the relatively intact and uninvaded 14,600 ha Berkshire Taconic Plateau in Massachusetts (USA). The Plateau comprises an ecologically

  9. CHARACTERIZATION OF THE FLORAL ODOR OF OREGON GRAPE: POSSIBLE FEEDING ATTRACTANTS FOR MOTHS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    It was hypothesized that flowers of Oregon grape, Berberis aquifolium Prursch., might release chemicals attractive to moths. Studies were conducted to determine what moths visit flowers of Oregon grape and to characterize the odor chemistry of those flowers in search of possible moth feeding attrac...

  10. AQUEOUS EXTRACT FROM DIFFERENT MEDICINAL PLANTS AS ANTICOCCIDIAL, GROWTH PROMOTIVE AND IMMUNOSTIMULANT IN BROILERS

    Microsoft Academic Search

    S. Ahmad; I. U. Jan; S. Gul

    2010-01-01

    A study was planned to investigate the role of aqueous extract from Garlic (Allium sativum), Ginger (Zingiber officinale), Neem (Azadirachta indica) and Berberry (Berberis lycium) fed in-mix for their effect upon growth performance, immunomostimulant and anticoccidial in broilers. Two hundred and fourty chicks were randomly allocated in to four groups A, B, C and D, and further into two subgroups,

  11. Growth response of native shrubs to acid mine spoil and to proposed soil amendments

    Microsoft Academic Search

    Pamela J. Voeller; Benjamin A. Zamora; James Harsh

    1998-01-01

    Successful reclamation of acid mine sites may be enhanced by revegetating with species that are tolerant to acid mine spoil conditions. This study was conducted to assess the response of four native shrub species, Amelanchier alnifolia Nutt., Symphoricarpos albus (L.) Blake, Berberis repens Lindl., and Ceanothus sanguineus Pursh, to 1) pyritic acid mine spoil amended with various levels of lime

  12. Carbon Dioxide Exchange by Several Streamside and Scrub Oak Community Species of Red Butte Canyon, Utah

    Microsoft Academic Search

    Stephen J. Dina; Lionel G. Klikoff

    2007-01-01

    ABSTRACT: Species of a xeric scrub oak community (scrub oak, Quercus gambelii; sagebrush, Artemisia tridentata; bitterbrush, Purshia have lower seasonal water potentials than species of a mesic streamside habitat (box elder, Acer negundo; bigtooth maple, Acer grandidentatum; creeping barberry, Berberis repens). The restriction of box elder to mesic sites is, in part, a function of its physiological responses to moisture

  13. Leaf litter decomposition and nitrogen concentration in decomposing leaves of a quercus seme?carpifolia smith forest stand of Kumaun Himalaya

    Microsoft Academic Search

    Laxmi Rawat; S. P. Singh

    1995-01-01

    Decomposition patterns of Quercus semecarpifolia (Kharsu oak; tree species), Viburnum cotinifolium, and Berberis asiatica (shrub species), Senecio rufinervis, Polygonum amplexicaule, and Selinum candollii (herb species) were studied. Sixty?five per cent weight loss occurred in Kharsu oak in 360 days, whereas total disappearance of leaf litter was achieved in 360 days in both the shrub species and in 210 days in

  14. Differences in Earthworm Densities and Nitrogen Dynamics in Soils Under Exotic and Native Plant Species

    Microsoft Academic Search

    P. S. Kourtev; W. Z. Huang; J. G. Ehrenfeld

    1999-01-01

    Previous studies of the invasion of two exotic plants – Berberis thunbergii and Microstegium vimineum – in hardwood forests of New Jersey have shown a significant increase of pH in soils under the invasive plants as compared with soils from under native shrubs (Vaccinium spp). We present a further investigation of soil properties under the exotic plants in question. We

  15. The antiamoebic effect of a crude drug formulation of herbal extracts against Entamoeba histolytica in vitro and in vivo

    Microsoft Academic Search

    Youvraj R. Sohni; Padmaja Kaimal; Ranjan M. Bhatt

    1995-01-01

    The antiamoebic effect of a crude drug formulation against Entamoeba histolytica was studied. In the traditional system of medicine in India, the formulation has been prescribed for intestinal disorders. It comprises of five medicinal herbs, namely, Boerhavia diffusa, Berberis aristata, Tinospora cordifolia, Terminalia chebula and Zingiber officinale. The dried and pulverized plants were extracted in ethanol together and individually. In

  16. [Ability of typical greenery shrubs of Beijing to adsorb and arrest PM2.5 ].

    PubMed

    Liang, Dan; Wang, Bin; Wang, Yun-qi; Zhang, Hui-lan; Yang, Song-nan; Li, Ang

    2014-09-01

    Four typical types of green shrubs of Beijing (Euonymus japonicus, Buxus microphylla, Berberis thunbergii cv. atropurpurea, Taxus cuspidate cv. nana) were selected to study their capacities in adsorbing and arresting PM2.5 using both field observations and air chamber simulations. Concurrently, in order to analyze the pollution characteristics of Beijing in winter and spring, the PM2.5 concentrations of December 2012 to May 2013 were collected. Experimental results showed that: From the gas chamber experiments, the ability to adsorb and arrest PM2.5 was in the order of Berberis thunbergii cv. atropurpurea > Buxus microphylla > Taxus cuspidate cv. nana > Euonymus japonicus, mainly due to the differences in leaf characteristics; Outside measurement results showed that the ability to adsorb and arrest PM2.5 was ranked as Buxus microphylla > Berberis thunbergii cv. atropurpurea > Taxus cuspidate cv. nana > Euonymus japonicus. Chamber simulation and outdoor observation showed that Buxus microphylla and Berberis thunbergii cv. atropurpurea had strong ability to adsorb and arrest PM2.5; Meanwhile, the slight differences between the chamber simulation and outdoor observation results might be related to plant structure. Compared to tree species, the planting condition of shrub species was loose, and it greened quickly; By analyzing the Beijing PM2.5 concentration values in winter and spring, it was found that the PM2.5 concentration was particularly high in the winter of Beijing, and evergreen shrubs maintained the ability to adsorb and arrest PM2.5. PMID:25518685

  17. Copyright 2010 by the Genetics Society of America DOI: 10.1534/genetics.110.120519

    E-print Network

    Swoboda, Peter

    alleles of fibulin (fbl-1), perlecan (unc-52), collagens (cle-1, dpy-7), laminins (lam-1, lam-3), one ADAM, or fibronectin, which are interconnected by molecules such as fibulin and the proteoglycans perlecan and nidogen

  18. Croissance du trfle violet (Trifolium pratense L.) en association avec du ray-grass d'Italie

    E-print Network

    Boyer, Edmond

    'Italie (Lolium multiflorum Lam. ssp. italicum). I. - Description de l'organisation morphologique du trèfle (1 with Italian rye-grass (Lolium multiflorum Lam. spp. italicum). I. — Morphological organization

  19. Plant pathology Effects of adjuvants on herbicidal action.

    E-print Network

    Paris-Sud XI, Université de

    increases diclofop-methyl efficacy against ryegrass (Lolium multiflorum Lam) at application volumes varying solvant et un mouillant améliore l'efficacité du diclofop-méthyl sur ray-grass ( Lolium multiflorum Lam and isoproturon against ryegrass (Lolium

  20. Plant pathology Effects of adjuvants on herbicidal action.

    E-print Network

    Paris-Sud XI, Université de

    increases isoproturon efficacy against ryegrass (Lolium multiflorum Lam) at application volumes of 300 solvant et un mouillant améliore l'efficacité de l'isoproturon sur ray-grass (Lolium multiflorum Lam) aux

  1. J. Math. Biol. DOI 10.1007/s00285-009-0269-4 Mathematical Biology

    E-print Network

    Othmer, Hans

    -mail: changlee@wpi.edu H. G. Othmer School of Mathematics, University of Minnesota, 270A Vincent Hall the time derivative of the concentration of fast species or radicals to be zero (Lam 1993; Lam and Goussis

  2. Membrane antigens of human bronchial epithelial cells identified by monoclonal antibodies

    Microsoft Academic Search

    S. Bernal; K. Weinberg; M. Kakefuda; R. Stahel; C. O'Hara; Y. C. Wong

    1988-01-01

    Summary  Subpopulations of normal bronchial epithelial cells were identified using a series of murine monoclonal antibodies. These\\u000a antibodies were used to stain intact bronchial epithelial cells in culture by indirect immunofluorescence. LAM 2 reacted with\\u000a 80%, LAM 6 with 75%, LAM 7 with 60%, and LAM 8 with 5% of these cells. Sections of human bronchial epithelium were also stained\\u000a with

  3. Is Urinary Lipoarabinomannan the Result of Renal Tuberculosis? Assessment of the Renal Histology in an Autopsy Cohort of Ugandan HIV-Infected Adults

    PubMed Central

    Cox, Janneke A.; Lukande, Robert L.; Kalungi, Sam; Van Marck, Eric; Van de Vijver, Koen; Kambugu, Andrew; Nelson, Ann M.; Colebunders, Robert; Manabe, Yukari C.

    2015-01-01

    Objective The detection of urinary lipoarabinomannan (LAM), a mycobacterial cell wall component, is used to diagnose tuberculosis (TB). How LAM enters the urine is not known. To investigate if urinary LAM-positivity is the result of renal TB infection we correlated the outcomes of urinary LAM-antigen testing to renal histology in an autopsy cohort of hospitalized, Ugandan, HIV-infected adults. Methods We performed a complete autopsy, including renal sampling, in HIV-infected adults that died during hospitalization after written informed consent was obtained from the next of kin. Urine was collected postmortem through post-mortem catheterisation or by bladder puncture and tested for LAM with both a lateral flow assay (LFA) and an ELISA assay. Two pathologists assessed the kidney histology. We correlated the LAM-assay results and the histology findings. Results Of the 13/36 (36%) patients with a positive urinary LAM ELISA and/or LFA, 8/13 (62%) had renal TB. The remaining 5 LAM-positive patients had disseminated TB without renal involvement. Of the 23 LAM-negative patients, 3 had disseminated TB without renal involvement. The remaining LAM-negative patients had no TB infection and died mostly of fungal and bacterial infections. LAM LFA had a sensitivity of 81% and specificity of 100% to diagnose TB at any location, and the LAM ELISA a sensitivity of 63% and a specificity of 100%. 54% (7/13) LAM LFA-positive patients were not on anti-TB treatment at the time of death. Conclusion Renal TB infection explained LAM-positivity in the majority of patients. Patients with disseminated TB without renal involvement can also be diagnosed with LAM. This suggests that other mechanisms that lead to urinary LAM-positivity exist in a minority of patients. PMID:25897661

  4. Construction and first applications of a global cost of fishing database

    E-print Network

    Pauly, Daniel

    Construction and first applications of a global cost of fishing database Vicky W. Y. Lam*, Ussif Around Us Project, Fisheries Centre, the University of British Columbia, 2202 Main Mall, Vancouver, BC.lam@fisheries.ubc.ca. Lam, V. W. Y., Sumaila, U. R., Dyck, A., Pauly, D., and Watson, R. 2011. Construction and first

  5. Original article Development of SCARs useful for species

    E-print Network

    Paris-Sud XI, Université de

    . multiflorum Lam. / Lolium perenne L. / RAPD marker / SCAR Résumé - Développement de SCAR pour l hasard) sur deux variétés de Lolium multiflorum Lam. et quatre de Lolium perenne L., dans le but de of agronomical- ly important temperate forage grass species. Lolium perenne L. and L. multiflorum Lam. are two

  6. Instrument-Specific Harmonic Atoms for Mid-Level Music Representation

    E-print Network

    Vincent, Ga¨el Richard, Senior Member, IEEE, and Laurent Daudet, Member, IEEE Abstract Several studies Daudet are with the University Pierre et Marie Curie-Paris 6, Institut Jean Le Rond d'Alembert, LAM team, 11 rue de Lourmel, F-75015 Paris, France (e-mail: leveau@lam.jussieu.fr, daudet@lam

  7. Numero d'ordre: 4446 presentee `a

    E-print Network

    Paris-Sud XI, Université de

    Physiques et de l'Ing´enieure par Yek Wah LAM (Yi Hua LAM ) Pour obtenir le grade de DOCTEUR Sp Xuan Lam , without whose consistent encouragement and total sacrifice, this thesis would have never AURENCHE (LAPTH, Annecy) whom I knew since 2003, and Vincent BRETON (LPC, Clermont-Ferrand) known in 2007

  8. L'UTILISATION DE MODLES INTERMDIAIRES DYNAMIQUES POUR LA SYNTHSE AUDIO-GRAPHIQUE

    E-print Network

    Paris-Sud XI, Université de

    Hugues Genevois Émilien Ghomi LAM - Institut Jean le Rond d'Alembert vincent@mazirkat.org LAM - InstitutL'UTILISATION DE MODÈLES INTERMÉDIAIRES DYNAMIQUES POUR LA SYNTHÈSE AUDIO-GRAPHIQUE Vincent Goudard Jean le Rond d'Alembert genevois@lam.jussieu.fr LRI ­ Université Paris Sud 11 emilien

  9. Characterization of a Truncated Lipoarabinomannan from the Actinomycete Turicella otitidis

    Microsoft Academic Search

    Martine Gilleron; Natalie J. Garton; Jerome Nigou; Therese Brando; Germain Puzo; Iain C. Sutcliffe

    2005-01-01

    Lipoarabinomannan (LAM) lipoglycans have been characterized from a range of mycolic acid-containing actinomycetes and from the amycolate actinomycete Amycolatopsis sulphurea. To further understand the struc- tural diversity of this family, we have characterized the lipoglycan of the otic commensal Turicella otitidis. T. otitidis LAM (TotLAM) has been determined to consist of a mannosyl phosphatidylinositol anchor unit carrying an ( 136)-linked

  10. Evaluation of surface roughness in laser-assisted machining of aluminum oxide ceramics with Taguchi method

    Microsoft Academic Search

    Chih-Wei Chang; Chun-Pao Kuo

    2007-01-01

    This paper evaluates laser-assisted machining (LAM) as an economically viable process for manufacturing precision aluminum oxide ceramic parts. Because it is locally heated by an intense laser source prior to material removal, LAM leads to higher material removal rates, as well as improved control of workpiece properties and geometry. To assess the feasibility of the LAM process and better understand

  11. Wild Type Mesenchymal Cells Contribute to the Lung Pathology of Lymphangioleiomyomatosis

    PubMed Central

    Clements, Debbie; Dongre, Arundhati; Krymskaya, Vera P.; Johnson, Simon R.

    2015-01-01

    Lymphangioleiomyomatosis (LAM) is a rare disease leading to lungs cysts and progressive respiratory failure. Cells of unknown origin accumulate in the lungs forming nodules and eventually resulting in lung cysts. These LAM cells are described as clonal with bi-allelic mutations in TSC-2 resulting in constitutive mTOR activation. However LAM nodules are heterogeneous structures containing cells of different phenotypes; we investigated whether recruited wild type cells were also present alongside mutation bearing cells. Cells were isolated from LAM lung tissue, cultured and characterised using microscopy, immunocytochemistry and western blotting. Fibroblast-like cells were identified in lung tissue using immunohistochemical markers. Fibroblast chemotaxis toward LAM cells was examined using migration assays and 3D cell culture. Fibroblast-like cells were obtained from LAM lungs: these cells had fibroblast-like morphology, actin stress fibres, full length tuberin protein and suppressible ribosomal protein S6 activity suggesting functional TSC-1/2 protein. Fibroblast Activation Protein, Fibroblast Specific Protein/S100A4 and Fibroblast Surface Protein all stained subsets of cells within LAM nodules from multiple donors. In a mouse model of LAM, tuberin positive host derived cells were also present within lung nodules of xenografted TSC-2 null cells. In vitro, LAM 621-101 cells and fibroblasts formed spontaneous aggregates over three days in 3D co-cultures. Fibroblast chemotaxis was enhanced two fold by LAM 621-101 conditioned medium (p=0.05), which was partially dependent upon LAM cell derived CXCL12. Further, LAM cell conditioned medium also halved fibroblast apoptosis under serum free conditions (p=0.03). Our findings suggest that LAM nodules contain a significant population of fibroblast-like cells. Analogous to cancer associated fibroblasts, these cells may provide a permissive environment for LAM cell growth and contribute to the lung pathology of LAM lung disease. PMID:25978616

  12. Molecular characterization of Capra hircus lysosomal ?-mannosidase and potential mutant site for the therapy of locoweed poisoning.

    PubMed

    Xiangya, Kong; Jiangye, Zhang; Ying, Wu; Jianfei, Li; Qinfan, Li

    2014-01-01

    Lysosomal ?-Mannosidase (LAM) belongs to the glycoside hydrolyzing enzymes family 38 and is involved in the biosynthesis and turnover of N-linked glycoproteins process. Locoweeds, which contain swainsonine (SW) that inhibits LAM, are the main poisoning plants in many regions of the world, and thereby resulting in animal poisoning or even death. Based on regions of protein sequence conservation between LAM from Bos taurus and Homo sapiens, we cloned cDNA encoding Capra hircus LAM (chLAM). Expression of cDNA in Pichia pastoris resulted in the secretion of aLAM activity into the culture medium. The recombinant chLAM was activated 1.6 and 1.2-fold with Zn(2+) and Ca(2+), respectively. By homology modeling, molecular docking and mutant analysis, we obtained the probable binding modes of SW at the allosteric sites of chLAM, and the potential mutant sites for the resistance to SW. Prediction of SW sensitivity to A28 W/G, D58 Y/G mutant chLAM is lower than wild type chLAM. The obtained results lead to a better understanding of not only interactions between substrate/SW and chLAM, but also of a potential strategy for a novel therapy for locoweed poisoning. PMID:24660168

  13. Effects of dehydrated methanol extracts of moringa ( Moringa oleifera Lam.) leaves and three of its fractions on growth performance and feed nutrient assimilation in Nile tilapia ( Oreochromis niloticus (L.))

    Microsoft Academic Search

    Euloge Dongmeza; Perumal Siddhuraju; George Francis; Klaus Becker

    2006-01-01

    A 10-week feeding trial was conducted in a recirculation system at (27±0.5 °C) to determine the effect of a methanol extract of moringa leaf meal and its different fractions. Nine isonitrogenous and isoenergetic diets containing 35% crude protein and 20 MJ kg?1 gross energy were used. All the experimental diets contained the same amount of fish meal. The inclusion of moringa methanol

  14. Energy E#cient Online Deadline Scheduling H.L. Chan # W.T. Chan # T.W. Lam # L.K. Lee # K.S. Mak # P. Wong +

    E-print Network

    Lam, Tak-Wah

    as these devices are battery­operated. A popular technology to reduce energy usage is to allow variable processor the energy to complete all jobs; O(1)­competitive online algorithms with respect to energy usage have been maintaining O(1)­competitive on energy usage. 1 Introduction Deadline scheduling. Let us first review

  15. Pictured, top to bottom: Leadership of the Ag Biotech Center: Peter Day (founding director and professor emeritus, followed by Gerben Zylsytra (Biochemistry and Microbiology), and Eric Lam (Plant Biology and

    E-print Network

    Goodman, Robert M.

    as a "virtual" instrumentation hub to promote research and education in the larger School and University instrumentation are also included in this renewed investment to bring state of the art tools to bear on our

  16. SKLOJET HÜCRESNDE BTÜMLÜ ?LAM KÖMÜRÜN FLOTASYONU VE JAMESON HÜCRES? ?LE FLOTASYON PERFORMANSLARININ KARILATIRILMASI Flotation of Bituminous Coal Slimes in the Cyclojet Cell and a Comparison of Cyclojet Cell and Jameson Cell in Terms of Their Flotation Performance

    Microsoft Academic Search

    Hasan HACIFAZLIOLU

    nd out whether it can be used in cleaning bituminous coal slimes. 80% of the bituminous coal slime used in the tests was ? ner than 53 ?m and it had an ash content of approximately 49%. By using the Cyclojet, the coal slimes yielded clean coal products having 11,32% ash content with 72,80% combustible matter recovery. Tests conducted in

  17. Local angular momentum-local impact parameter analysis: Derivation and properties of the fundamental identity, with applications to the F +H2, H +D2, and Cl +HCl chemical reactions

    NASA Astrophysics Data System (ADS)

    Monks, P. D. D.; Xiahou, Chengkui; Connor, J. N. L.

    2006-10-01

    The technique of local angular momentum-local impact parameter (LAM-LIP) analysis has recently been shown to provide valuable dynamical information on the angular scattering of chemical reactions under semiclassical conditions. The LAM-LIP technique exploits a nearside-farside (NF) decomposition of the scattering amplitude, which is assumed to be a Legendre partial wave series. In this paper, we derive the "fundamental NF LAM identity," which relates the full LAM to the NF LAMs (there is a similar identity for the LIP case). Two derivations are presented. The first uses complex variable techniques, while the second exploits an analogy between the motion of the scattering amplitude in the Argand plane with changing angle and the classical mechanical motion of a particle in a plane with changing time. Alternative forms of the fundamental LAM-LIP identity are described, one of which gives rise to a CLAM-CLIP plot, where CLAM denotes (Crosssection)×LAM and CLIP denotes (Crosssection)×LIP. Applications of the NF LAM theory, together with CLAM plots, are reported for state-to-state transitions of the benchmark reactions F +H2?FH+H, H +D2?HD+D, and Cl +HCl?ClH+Cl, using as input both numerical and parametrized scattering matrix elements. We use the fundamental LAM identity to explain the important empirical observation that a NF cross section analysis and a NF LAM analysis provide consistent (and complementary) information on the dynamics of chemical reactions.

  18. Structural features of lipoarabinomannan from Mycobacterium bovis BCG. Determination of molecular mass by laser desorption mass spectrometry.

    PubMed

    Venisse, A; Berjeaud, J M; Chaurand, P; Gilleron, M; Puzo, G

    1993-06-15

    It was recently shown that mycobacterial lipoarabinomannan (LAM) can be classified into two types (Chatterjee, D., Lowell, K., Rivoire B., McNeil M. R., and Brennan, P. J. (1992) J. Biol. Chem. 267, 6234-6239) according to the presence or absence of mannosyl residues (Manp) located at the nonreducing end of the oligoarabinosyl side chains. These two types of LAM were found in a pathogenic Mycobacterium tuberculosis strain and in an avirulent M. tuberculosis strain, respectively, suggesting that LAM with Manp characterizes virulent and "disease-inducing strains." We now report the structure of the LAM from Mycobacterium bovis Bacille Calmette-Guérin (BCG) strain Pasteur, largely used throughout the world as vaccine against tuberculosis. Using an up-to-date analytical approach, we found that the LAM of M. bovis BCG belongs to the class of LAMs capped with Manp. By means of two-dimensional homonuclear and heteronuclear scalar coupling NMR analysis and methylation data, the sugar spin system assignments were partially established, revealing that the LAM contained two types of terminal Manp and 2-O-linked Manp. From the following four-step process: (i) partial hydrolysis of deacylated LAM (dLAM), (ii) oligosaccharide derivatization with aminobenzoic ethyl ester, (iii) HPLC purification, (iv) FAB/MS-MS analysis; it was shown that the dimannosyl unit alpha-D-Manp-(1-->2)-alpha-D-Manp is the major residue capping the termini of the arabinan of the LAM. In this report, LAM molecular mass determination was established using matrix-assisted UV-laser desorption/ionization mass spectrometry which reveals that the LAM molecular mass is around 17.4 kDa. The similarity of the LAM structures between M. bovis BCG and M. tuberculosis H37Rv is discussed in regard to their function in the immunopathology of mycobacterial infection. PMID:8509380

  19. Inferring the contribution of sexual reproduction, migration and off-season survival to the temporal maintenance of microbial populations: a case study on the wheat fungal pathogen Puccinia striiformis f.sp. tritici.

    PubMed

    Ali, Sajid; Gladieux, Pierre; Rahman, Hidayatur; Saqib, Muhammad S; Fiaz, Muhammad; Ahmad, Habib; Leconte, Marc; Gautier, Angélique; Justesen, Annemarie F; Hovmøller, Mogens S; Enjalbert, Jérôme; de Vallavieille-Pope, Claude

    2014-02-01

    Understanding the mode of temporal maintenance of plant pathogens is an important domain of microbial ecology research. Due to the inconspicuous nature of microbes, their temporal maintenance cannot be studied directly through tracking individuals and their progeny. Here, we suggest a series of population genetic analyses on molecular marker variation in temporally spaced samples to infer about the relative contribution of sexual reproduction, off-season survival and migration to the temporal maintenance of pathogen populations. We used the proposed approach to investigate the temporal maintenance of wheat yellow rust pathogen, Puccinia striiformis f.sp. tritici (PST), in the Himalayan region of Pakistan. Multilocus microsatellite genotyping of PST isolates revealed high genotypic diversity and recombinant population structure across all locations, confirming the existence of sexual reproduction in this region. The genotypes were assigned to four genetic groups, revealing a clear differentiation between zones with and without Berberis spp., the alternate host of PST, with an additional subdivision within the Berberis zone. The lack of any differentiation between samples across two sampling years, and the very infrequent resampling of multilocus genotypes over years at a given location was consistent with limited over-year clonal survival, and a limited genetic drift. The off-season oversummering population in the Berberis zone, likely to be maintained locally, served as a source of migrants contributing to the temporal maintenance in the non-Berberis zone. Our study hence demonstrated the contribution of both sexual recombination and off-season oversummering survival to the temporal maintenance of the pathogen. These new insights into the population biology of PST highlight the general usefulness of the analytical approach proposed. PMID:24354737

  20. Effect of berbamine on T-cell mediated immunity and the prevention of rejection on skin transplants in mice

    Microsoft Academic Search

    Chong-Nian Luo; Xin Lin; Wen-Kui Li; Feng Pu; Li-Wei Wang; Shu-Sheng Xie; Pei-Gen Xiao

    1998-01-01

    Berbamine, an ingredient of Berberis, which itself is widely utilized in Chinese folk-medicine has been used as a source of leukogenics, anti-arrhythmics and anti-hypertensives. In recent years, the immunosuppressive effects of berbamine has been demonstrated. In order to further investigate the value of berbamine as an immunosuppressive agent, the delayed type hypersensitivity reaction (DTH) response with sheep red blood cells

  1. Photosynthesis and water-use efficiency: A comparison between invasive (exotic) and non-invasive (native) species

    Microsoft Academic Search

    KATE G. MCALPINE; LINLEY K. JESSON; DAVID S. KUBIEN

    2008-01-01

    Invasive species have been hypothesized to out-compete natives though either a Jack-of-all-trades strategy, where they are able to utilize resources effectively in unfavourable environments, a master-of-some, where resource utilization is greater than its competitors in favourable environments, or a combination of the two (Jack-and-master). We examined the invasive strategy of Berberis darwinii in New Zealand compared with four co-occurring native

  2. Quality Evaluation of Ayurvedic Crude Drug Daruharidra, Its Allied Species, and Commercial Samples from Herbal Drug Markets of India

    PubMed Central

    Srivastava, Sharad; Rawat, A. K. S.

    2013-01-01

    Berberis aristata known as “Daruharidra” in Ayurveda is a versatile medicinal plant used singly or in combination with other medicinal plants for treating a variety of ailments like jaundice, enlargement of spleen, leprosy, rheumatism, fever, morning/evening sickness, snakebite, and so forth. A major bioactive marker of this genus is an alkaloid berberine, which is known for its activity against cholera, acute diarrhea, amoebiasis, and latent malaria and for the treatment of oriental sore caused by Leishmania tropica. Although the roots of B. aristata are considered as the official drug (Ayurvedic Pharmacopoeia of India), the study revealed that different species of Berberis, namely. B. asiatica, B. chitria, and B. lycium are also used under the name of Daruharidra in different parts of the country. Detailed physicochemical and phytochemical studies of subjects like total ash, acid insoluble ash, tannins, and total alkaloids were calculated from the shade dried powdered material according to the recommended procedures. Further, heavy metal studies and quantitative estimation of berberine through HPTLC have also been performed as per ICH guidelines. A detailed study of four Berberis species, namely B. aristata, B. asiatica, B. chitria, and B. lycium, which are implicated as Daruharidra and collected from wild and ten commercial samples procured from various important drug markets in India has been carried out, which may be useful to pharmaceutical industries for the authentication of the commercial samples and exploring the possibilities of using other species as a substitute of B. aristata. PMID:23431340

  3. Studies on quantitative determination of total alkaloids and berberine in five origins of crude medicine "Sankezhen".

    PubMed

    Li, Luyang; Long, Weifang; Wan, Xiangluan; Ding, Qi; Zhang, Fei; Wan, Dingrong

    2015-02-01

    The roots of Berberis plants are widely used as a traditional Chinese medicine called "Sankezhen", having the activities of antibacterial and anti-inflammatory, and the ingredients are alkaloids. This work aims to study and compare the total alkaloids and individual alkaloid (berberine) contents in roots and stems from five origins of Berberis plants (Berberis soulieana Schneid., B. henryana Schneid., B. triacanthophora Fedde, B. gagnepainii Schneid. and B. bergmanniae Schneid.) and provides some references for resource and quality evaluation of the medicine. Acid dye colorimetry and high-performance liquid chromatography were used in the determination. The results showed that the contents for the total alkaloids in root and stem samples were in the range of 1.60-4.72% and 0.76-2.70%, while those of the berberine were 0.70-2.92% and 0.23-1.07%. With higher contents of the total alkaloids and berberine, the roots of B. soulieana, B. gagnepainii and B. bergmanniae were good sources of "Sankezhen". Meanwhile, the contents were also high in stems of the three plants, indicating that the stems were likely to be alternative sources of "Sankezhen" after further research. As the results of precision, stability and recovery tests shown, the methods were simple, rapid and reliable, and provided valuable basis for quality evaluation and new resource investigation of "Sankezhen". PMID:25013028

  4. Lymphangioleiomyomatosis — a wolf in sheep’s clothing

    PubMed Central

    Henske, Elizabeth P.; McCormack, Francis X.

    2012-01-01

    Lymphangioleiomyomatosis (LAM) is a rare progressive lung disease of women. LAM is caused by mutations in the tuberous sclerosis genes, resulting in activation of the mTOR complex 1 signaling network. Over the past 11 years, there has been remarkable progress in the understanding of LAM and rapid translation of this knowledge to an effective therapy. LAM pathogenic mechanisms mirror those of many forms of human cancer, including mutation, metabolic reprogramming, inappropriate growth and survival, metastasis via blood and lymphatic circulation, infiltration/invasion, sex steroid sensitivity, and local and remote tissue destruction. However, the smooth muscle cell that metastasizes, infiltrates, and destroys the lung in LAM arises from an unknown source and has an innocent histological appearance, with little evidence of proliferation. Thus, LAM is as an elegant, monogenic model of neoplasia, defying categorization as either benign or malignant. PMID:23114603

  5. Correlation between Mechanical Strength and Surface Conditions of Laser Assisted Machined Silicon Nitride

    Microsoft Academic Search

    F. M. Sciammarella; M. J. Matusky

    \\u000a High power fiber-coupled diode lasers for Laser-Assisted Machining (LAM) of ceramics provides an efficient, cost effective\\u000a solution for surface finishing of ceramic products. This paper presents experimental evidence of advantages of LAM over the\\u000a traditional diamond wheel grinding, a standard technique currently utilized in the finishing of ceramic surfaces. LAM, utilizing\\u000a fiber-coupled diode lasers, also provides advantages over other types

  6. Impact of prior lamivudine use on the antiviral efficacy and development of resistance to entecavir in chronic hepatitis B patients

    PubMed Central

    Hwang, Joo An; Kim, Kee Bum; Yang, Min Jae; Lim, Sun Gyo; Hwang, Jae Chul; Cheong, Jae Youn; Cho, Sung Won

    2015-01-01

    Background/Aims To determine the efficacies of entecavir (ETV) in nucleos(t)ide analogue (NA)-naïve chronic hepatitis B (CHB) patients and in those with prior lamivudine (LAM) use who did not develop resistance. Methods We retrospectively enrolled 337 patients with CHB who were treated with ETV (0.5 mg daily) for at least 30 months. The study included 270 (80.1%) NA-naïve patients and 67 (19.9%) LAM-use patients. Ten of the LAM-use patients were refractory to LAM therapy without developing resistance. Results Genotypic resistance to ETV developed more frequently in the LAM-use group (13.1%) than in the NA-naïve group (2.6%) at 60 months (P=0.009). In subgroup analysis, after excluding the 10 patients who were refractory to LAM therapy, the cumulative probability of ETV resistance did not differ significantly between the two groups (P=0.149). Prior LAM refractoriness and a higher hepatitis B virus DNA level at month 12 were independent predictive factors for the development of ETV resistance. Conclusions ETV resistance developed more frequently in LAM-use patients with CHB. However, prior LAM use without refractoriness did not affect the development of ETV resistance. The serum hepatitis B virus DNA level at month 12 was a major predictor for the development of ETV resistance. PMID:26157750

  7. Liposomal delivery of lipoarabinomannan triggers Mycobacterium tuberculosis specific T-cells.

    PubMed

    Kallert, Stephanie; Zenk, Sebastian F; Walther, Paul; Grieshober, Mark; Weil, Tanja; Stenger, Steffen

    2015-07-01

    Lipoarabinomannan (LAM) is a major cell wall component of Mycobacterium tuberculosis (Mtb). LAM specific human T-lymphocytes release interferon-? (IFN?) and have antimicrobial activity against intracellular Mtb suggesting that they contribute to protection. Therefore the induction of LAM-specific memory T-cells is an attractive approach for the design of a new vaccine against tuberculosis. A prerequisite for the activation of LAM-specific T-cells is the efficient uptake and transport of the glycolipid antigen to the CD1 antigen presenting machinery. Based on the hydrophobicity of LAM we hypothesized that packaging of LAM into liposomes will support the activation of T-lymphocytes. We prepared liposomes containing phosphatidylcholine, cholesterol, stearylated octaarginine and LAM via thin layer hydration method (LIPLAM). Flow cytometry analysis using fluorescently labelled LIPLAM showed an efficient uptake by antigen presenting cells. LAM delivered via liposomes was biologically active as demonstrated by the down-regulation of peroxisome proliferator activated receptor gamma (PPAR?) protein expression. Importantly, LIPLAM induced higher IFN? production by primary human T-lymphocytes than purified LAM (2-16 times) or empty liposomes. These results suggest that the delivery of mycobacterial glycolipids via liposomes is a promising approach to promote the induction of M. tuberculosis specific T-cell responses. PMID:26043674

  8. Opposing effects of interferon-gamma on iNOS and interleukin-10 expression in lipopolysaccharide- and mycobacterial lipoarabinomannan-stimulated macrophages.

    PubMed Central

    Roach, T I; Barton, C H; Chatterjee, D; Liew, F Y; Blackwell, J M

    1995-01-01

    Previous studies have demonstrated that, like bacterial lipopolysaccharide (LPS), arabinofuranosyl-terminated lipoarabinomannan (AraLAM) from an attenuated strain of Mycobacterium induces potent early gene (c-fos, KC, JE and TNF-alpha) responses in murine macrophages, whereas extensively alpha-Manp capped LAM (ManLAM) from virulent M. tuberculosis do not. In this study we have extended analysis of the influence of mycobacterial LAM on macrophage function by demonstrating that AraLAM (but not ManLAM), like bacterial LPS, is a potent stimulator of inducible nitric oxide synthase (iNOS) expression independent of the autocrine activity of co-stimulated tumour necrosis factor-alpha (TNF-alpha) release. The inability of ManLAM to induce iNOS expression was not due to induction of the 'deactivating' cytokine interleukin-10 (IL-10). Indeed, like LPS, AraLAM was also a potent inducer of IL-10 expression. However, analysis of AraLAM- or LPS-induced responses in the presence of interferon-gamma (IFN-gamma) showed that, whereas IFN-gamma acts as a potent co-stimulus for iNOS, it completely inhibits the IL-10 response. Hence, the presence of IFN-gamma early in infection will have an important immunomodulatory role in determining the macrophage response. These results have important implications for the pathogenesis of virulent and avirulent mycobacteria in vivo. Images Figure 1 Figure 2 Figure 3 PMID:7543444

  9. Initial report on the application of laser ablation - inductively coupled plasma mass spectrometry for the analysis of radioactive Hanford Tank Waste materials

    SciTech Connect

    Smith, M.R.; Hartman, J.S.; Alexander, M.L.; Mendoza, A.; Hirt, E.H.; Stewart, T.L.; Hansen, M.A.; Park, W.R.; Peters, T.J.; Burghard, B.J.

    1996-12-01

    Initial LA/MS analyses of Hanford tank waste samples were performed successfully using laboratory and hot cell LA/MS instrumentation systems. The experiments described in this report have demonstrated that the LA/MS data can be used to provide rapid analysis of solid, radioactive Hanford tank waste samples to identify major, minor, and trace constituents (elemental and isotopic) and fission products and radioactive isotopes. The ability to determine isotopic constituents using the LA/MS method yielded significant advantages over ICP/AES analysis by providing valuable information on fission products and radioactive constituents.

  10. UNIVERSIT LILLE NORD DE FRANCE COLE DOCTORALE BIOLOGIE & SANT

    E-print Network

    Paris-Sud XI, Université de

    Denis, Ping H. Lam, Nathalie Bouloutte, Manon Robert, Stéphane Grimault, Louis de Beaumont, Sébastien Robaye, Vincent Vangelder, Charles Gaudfroy, William Vandamme, Matthieu Deprieck, Jérémie Vandenbunder

  11. Partenaires : la Haute Ecole Charlemagne, la Haute Ecole de la Province de Lige, la Haute Ecole de la Ville de Lige, la Haute Ecole Libre Mosane, la Haute Ecole Robert Schuman et l'Universit de Lige.

    E-print Network

    Liège, Université de

    plantes psychotropes sont à l'origine des drogues les plus consommées actuellement: - Pavot (Papaver somniferum L.); - Chanvre (Cannabis sativa L.); - Coca (Erythrocylum coca Lam). Les aspects historiques

  12. Molecular Genetic Analysis of the Nested Drosophila melanogaster Lamin C Gene

    PubMed Central

    Schulze, Sandra R.; Curio-Penny, Beatrice; Li, Yuhong; Imani, Reza A.; Rydberg, Lena; Geyer, Pamela K.; Wallrath, Lori L.

    2005-01-01

    Lamins are intermediate filaments that line the inner surface of the nuclear envelope, providing structural support and making contacts with chromatin. There are two types of lamins, A- and B-types, which differ in structure and expression. Drosophila possesses both lamin types, encoded by the LamC (A-type) and lamin Dm0 (B-type) genes. LamC is nested within an intron of the essential gene ttv. We demonstrate that null mutations in LamC are lethal, and expression of a wild-type LamC transgene rescues lethality of LamC but not ttv mutants. Mutations in the human A-type lamin gene lead to diseases called laminopathies. To determine if Drosophila might serve as a useful model to study lamin biology and disease mechanisms, we generated transgenic flies expressing mutant LamC proteins modeled after human disease-causing lamins. These transgenic animals display a nuclear lamin aggregation phenotype remarkably similar to that observed when human mutant A-type lamins are expressed in mammalian cells. LamC aggregates also cause disorganization of lamin Dm0, indicating interdependence of both lamin types for proper lamina assembly. Taken together, these data provide the first detailed genetic analysis of the LamC gene and support using Drosophila as a model to study the role of lamins in disease. PMID:15965247

  13. Inheritance and OMT: a CSP approach Chan Wing Kwong

    E-print Network

    Chan, Wing-Kwong Ricky

    flourished in this project. Sincere thanks should equally and dedicatedly be given to Dr T.Y. Chen, Dr F.C Maria Lam, Ms Smitty Lam, Ms Brenda Ng, Ms Joanne Chan, Ms Priscilla Chung, Ms Veronica Yim, Ms Irene

  14. MONITORING AND DECISION MAKING FOR CUCUMBER BEETLES ON MUSKMELON

    E-print Network

    Ginzel, Matthew

    MONITORING AND DECISION MAKING FOR CUCUMBER BEETLES ON MUSKMELON Frankie Lam and Ricky E. Foster) Striped cucumber beetle and (B) Spotted cucum- ber beetle or southern corn rootworm beetle. (Photo credit: Frankie Lam, Purdue University) Striped and spotted cucumber beetles (Figs. 1 A and B) are the most

  15. LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis

    PubMed Central

    Blumenthal, Antje; Bhamidi, Suresh; Gibbs, Sara; Jackson, Mary; Zare, Richard N.; Ehrt, Sabine; Ernst, Joel D.; Banaei, Niaz

    2014-01-01

    Mycobacterium tuberculosis employs various virulence strategies to subvert host immune responses in order to persist and cause disease. Interaction of M. tuberculosis with mannose receptor on macrophages via surface-exposed lipoarabinomannan (LAM) is believed to be critical for cell entry, inhibition of phagosome-lysosome fusion, and intracellular survival, but in vivo evidence is lacking. LprG, a cell envelope lipoprotein that is essential for virulence of M. tuberculosis, has been shown to bind to the acyl groups of lipoglycans but the role of LprG in LAM biosynthesis and localization remains unknown. Using an M. tuberculosis lprG mutant, we show that LprG is essential for normal surface expression of LAM and virulence of M. tuberculosis attributed to LAM. The lprG mutant had a normal quantity of LAM in the cell envelope, but its surface was altered and showed reduced expression of surface-exposed LAM. Functionally, the lprG mutant was defective for macrophage entry and inhibition of phagosome-lysosome fusion, was attenuated in macrophages, and was killed in the mouse lung with the onset of adaptive immunity. This study identifies the role of LprG in surface-exposed LAM expression and provides in vivo evidence for the essential role surface LAM plays in M. tuberculosis virulence. Findings have translational implications for therapy and vaccine development. PMID:25232742

  16. Ragam: Sri Ranjani (22nd mela Kharaharapriya janyam) ARO: S R2 G2 M1 D2 N2 S ||

    E-print Network

    Kalyanaraman, Shivkumar

    N - dn | NS , nD ssnd m- d N || Shan kara Ba-- la Lam bo- dhara Sun-- dara #12;snrs ndsn dmnd mr G || kara Ba-- la - Lam bo- dhara Sun-- dara snrs ndsn dmnd mr G | gr rs , r G M ; ; gm || Gaja va-da- na Sun-- dara Anupallavi: Ajanamarendranu Munivarum Paniyum Pankaja Charana Charanam Charanam You

  17. American Institute of Aeronautics and Astronautics

    E-print Network

    Hirani, Anil N.

    and captures at Europa are explored using a real ephemeris. The path from Ganymede to Europa has many branches TO AND CAPTURES AT EUROPA Try Lam and Anil N. Hirani and Julie A. Kangas 16th AAS/AIAA Space Flight Mechanics #12;AAS 06-188 CHARACTERISTICS OF TRANSFERS TO AND CAPTURES AT EUROPA Try Lam and Anil N. Hirani

  18. Nested regional simulation of climate change over the Alps for the scenario of a doubled greenhouse forcing

    Microsoft Academic Search

    M. W. Rotach; M. R. Marinucci; M. Wild; P. Tschuck; A. Ohmura; M. Beniston

    1997-01-01

    Simulated temperature and precipitation changes over western Europe for a scenario of doubled atmospheric concentrations of CO2 are presented. The simulations are performed using a Limited Area Model LAM (RegCM2) nested into a General Circulation Model (ECHAM3). Both model components are operated at very high spatial resolutions — approximately 120 km for the GCM and 20 km for the LAM;

  19. Recherche des facteurs responsables du dclenchement du tallage chez le ray-grass d'Italie (Lolium multiflorum

    E-print Network

    Boyer, Edmond

    -grass (Lolium multiflorum lam.) under field conditions. The relationship between climate and time from emergenceRecherche des facteurs responsables du déclenchement du tallage chez le ray-grass d'Italie (Lolium multiflorum lam.) en conditions naturelles Jean-Claude SIMON Richard DELECOLLE LN.R.A., Station d'Agronomie, 4

  20. Research on the remote control simulation for the Loitering Attack Missile based on the distributed VR technique

    Microsoft Academic Search

    Shengzhi Yuan; Xiaofang Xie; Aidong Liu; Jian Cao

    2008-01-01

    In order to master the remote control technology of the Loitering Attack Missile (LAM), and the application of the data link into the cruise missile, it is very important to develop a remote control simulation for the LAM. The structure of the simulation system was designed on the latform of the High Level Architecture (HLA). The key questions such as

  1. Bulletin of Mathematical Biology (2008) 70: 13261347 DOI 10.1007/s11538-008-9301-x

    E-print Network

    Glass, Leon

    2008-01-01

    Bulletin of Mathematical Biology (2008) 70: 1326­1347 DOI 10.1007/s11538-008-9301-x ORIGINAL). This spontaneous variability is correlated to ventricular contraction (Lam- mers et al., 1991; Ravelli et al., 1994) and respiration (Waxman et al., 1991). Lam- mers et al. (1991) observed that the prolongation of the atrial

  2. 3, 30613097, 2006 Meteorological

    E-print Network

    Paris-Sud XI, Université de

    HESSD 3, 3061­3097, 2006 Meteorological analogues to account for LAM QPF uncertainty T. Diomede et System Sciences The use of meteorological analogues to account for LAM QPF uncertainty T. Diomede 1 , F. Nerozzi 1 , T. Paccagnella 1 , and E. Todini 2 1 Regional Hydro-Meteorological Service ARPA-SIM, Bologna

  3. Efficacy of Lamivudine or Entecavir on Acute Exacerbation of Chronic Hepatitis B

    PubMed Central

    Kanda, Tatsuo; Shinozaki, Masami; Kamezaki, Hidehiro; Wu, Shuang; Nakamoto, Shingo; Arai, Makoto; Fujiwara, Keiichi; Goto, Nobuaki; Imazeki, Fumio; Yokosuka, Osamu

    2012-01-01

    Background/Aims: Spontaneous acute exacerbation of chronic hepatitis B virus (HBV) infection occasionally occurs in its natural history, sometimes leading rapidly to fatal hepatic failure. We compared the effects of lamivudine (LAM) with those of entecavir (ETV) treatments in acute exacerbation of chronic hepatitis B with 500 IU/L or higher alanine aminotransferase (ALT) levels. Methods: Thirty-four patients with acute exacerbation were consecutively treated with LAM /ETV. Their clinical improvements were compared. Results: Among LAM-treated and ETV-treated patients, none showed a reduction of <1 log IU/mL in HBV DNA after 1 or 3 months of treatment. Initial virological response, defined as a reduction of 4 log IU/mL in HBV DNA at 6 months, with LAM and ETV, respectively, was 83.3% and 100%. One LAM patient developed hepatic encephalopathy, but all patients in both groups survived. Twelve months after treatment, 41.6% of 24 LAM group patients switched to another drug or added adefovir to their treatment due to the emergence of LAM-resistant mutants. On the other hand, patients receiving ETV did not need to change drugs. Conclusions: ETV appears to be as effective as LAM in the treatment of patients with acute exacerbation of chronic hepatitis B. Clinicians should carefully start to treat these patients as soon as possible. PMID:22211086

  4. STUDENT ORGANIZATIONS MEMBERS OF THE EXECUTIVE LEADERSHIP COUNCIL (ELC) COLLEGE OF BUSINESS, FALL 2013

    E-print Network

    Su, Xiao

    ADVISORS PRESIDENT EMAIL ADDRESS (please use all listed) PHONE NUMBER 1. AIESEC Jeffrey Gaines Amie lam* sanjosepresident@aiesecus.org amie.lam@aiesec.net 2. Alpha Kappa Psi (AKP) Richard Kepple Tim Hill Steven Pamintuan. Management Information Systems Association (MISA) Tim Hill Richard Burkhard Vincent Lee* president

  5. 116 IEEE TRANSACTIONS ON AUDIO, SPEECH, AND LANGUAGE PROCESSING, VOL. 16, NO. 1, JANUARY 2008 Instrument-Specific Harmonic Atoms for

    E-print Network

    Paris 7 - Denis Diderot, Université

    , France (e-mail: daudet@lam. jussieu.fr). Emmanuel Vincent is with the METISS Project, IRISA-INRIA, 35042 Instrument-Specific Harmonic Atoms for Mid-Level Music Representation Pierre Leveau, Emmanuel Vincent, Member. P. Leveau was with the Institut Jean Le Rond d'Alembert, LAM team, Univer- sity Pierre et Marie

  6. UNIVERSITE DE PARIS-EST pour l'obtention du grade de

    E-print Network

    Paris-Sud XI, Université de

    vu le jour. Ensuite, je remercie également Vincent PENSEE de m'avoir donné des encadrements au long Laboratoire de Mécanique (LAM), Christian SOIZE et Guy BONNET, de m'avoir accueilli et offert des conditions mes remerciements à tous les autres membres du LAM et à tous ceux qui m'ont aidé de près ou de loin

  7. On the extinction of Continuous State Branching Processes with catastrophes

    E-print Network

    On the extinction of Continuous State Branching Processes with catastrophes Vincent Bansaye 1 and continuous state space. Such vincent.bansaye@polytechnique.edu jcpardo@cimat.mx charline] and studied by many authors included Bingham [Bin76], Grey [Gre74], Grimvall [Gri74], Lamperti [Lam67a, Lam67b

  8. Mid-level sparse representations for timbre identification

    E-print Network

    Richard, Gaël

    -specific harmonic dictionary Pierre Leveau pierre.leveau@enst.fr Emmanuel Vincent emmanuel.vincent@elec.qmul.ac.uk Ga¨el Richard gael.richard@enst.fr Laurent Daudet daudet@lam.jussieu.fr GET/ENST, 37-39 rue Dareau, 75014 Paris ­ France LAM (Universit´e Pierre et Marie Curie), 11, rue de Lourmel, 75015 Paris ­ France

  9. On the extinction of Continuous State Branching Processes with catastrophes

    E-print Network

    Paris-Sud XI, Université de

    On the extinction of Continuous State Branching Processes with catastrophes Vincent Bansaye 1 authors included Bingham [Bin76], Grey vincent.bansaye@polytechnique.edu jcpardo@cimat.mx charline.smadi@polytechnique.edu 1 hal-00781203,version2-24May2013 #12;[Gre74], Grimvall [Gri74], Lamperti [Lam67a, Lam67b], to name

  10. Teacher Ratings of Evidence-Based Practices from the Field of Autism

    ERIC Educational Resources Information Center

    Borders, Christina M.; Bock, Stacey Jones; Szymanski, Christen

    2015-01-01

    Students who have a hearing loss and a comorbid diagnosis of an autism spectrum disorder (ASD) have multiple obstacles to overcome. Using Gallaudet Research Institute data, Szymanski, Brice, Lam, and Hotto calculated 1 deaf student in 59 received services for both a hearing loss and an ASD (Szymanski, Brice, Lam, & Hotto, 2012). Teachers of…

  11. SHARKS, SKATES, RAYS, AND CHIMAERAS

    E-print Network

    SHARKS, SKATES, RAYS, AND CHIMAERAS UNITED STATES DEPARTMENT OF THE INTERIOR FISH AND WILDLIFE of sharks Species Mucimum length Muimum length Scientific na.rr;e (measure 11 inches Porbeagle ·....... 1 LamTUl TUlSUS........... ... 10 feet 12 feet Sall10n shark. .... Lam

  12. Lindenfors, Revell & Nunn (2010) Supporting Information 1 Supporting Information to

    E-print Network

    Revell, Liam

    2010-01-01

    (lambda,logL(:,MLpos(2)),'k'); xlabel(lam,'fontname','symbol'); ylabel('log(L)'); title('C) '); subplot(2,2,4); plot(epsilon MAX] values for grid search on epsilon (eps) and lambda % (lam). % Returns dbar, tval, P (P-value), MLEs for epsilon, sigma^2, and lambda, % and the log-likelihood. % Optimization is performed on a 100 x

  13. The mannose cap of mycobacterial lipoarabinomannan does not dominate the Mycobacterium–host interaction

    Microsoft Academic Search

    B. J. Appelmelk; J. den Dunnen; N. N. Driessen; R. Ummels; M. Pak; J. Nigou; G. Larrouy-Maumus; S. S. Gurcha; F. Movahedzadeh; J. Geurtsen; E. J. Brown; M. M. Eysink Smeets; G. S. Besra; P. T. J. Willemsen; T. L. Lowary; Y. van Kooyk; J. J. Maaskant; N. G. Stoker; P. van der Ley; G. Puzo; C. M. J. E. Vandenbroucke-Grauls; C. W. Wieland; T. van der Poll; T. B. H. Geijtenbeek; Sar van der A. M; W. Bitter

    2008-01-01

    Pathogenic mycobacteria have the ability to persist in phagocytic cells and to suppress the immune system. The glycolipid lipoarabinomannan (LAM), in particular its mannose cap, has been shown to inhibit phagolysosome fusion and to induce immunosuppressive IL-10 production via interaction with the mannose receptor or DC-SIGN. Hence, the current paradigm is that the mannose cap of LAM is a crucial

  14. The Diagnostic Accuracy of Urine Lipoarabinomannan Test for Tuberculosis Screening in a South African Correctional Facility

    PubMed Central

    Hanifa, Yasmeen; Telisinghe, Lilanganee; Fielding, Katherine L.; Malden, Justin L.; Churchyard, Gavin J.; Grant, Alison D.; Charalambous, Salome

    2015-01-01

    Background We evaluated the diagnostic accuracy of the urine lipoarabinomannan (LAM) antigen detection assay (Clearview TB-ELISA) to screen for tuberculosis in a South African correctional facility. Methods Between September 2009 and October 2010, male offenders were screened for tuberculosis (symptoms, chest radiograph, two spot sputum specimens for microscopy and culture), and urine tested for LAM. Sensitivity, specificity and predictive values of LAM were calculated using definite and probable tuberculosis combined as our gold standard. Findings 33/871 (3.8%) participants (26% HIV-positive) had tuberculosis. Amongst HIV-positive vs. HIV-negative offenders the sensitivity and specificity of LAM was 7.1% vs. 0% and 98.5% vs. 99.8% respectively. Conclusion Urine LAM ELISA has inadequate sensitivity for TB screening in this population. PMID:26010840

  15. The Loss of Tuberin Promotes Cell Invasion through the ?-Catenin Pathway

    PubMed Central

    Barnes, Elizabeth A.; Kenerson, Heidi L.; Mak, Baldwin C.; Yeung, Raymond S.

    2010-01-01

    Mutations in the tumor suppressor tuberin (TSC2) are a common factor in the development of lymphangioleiomyomatosis (LAM). LAM is a cystic lung disease that is characterized by the infiltration of smooth muscle–like cells into the pulmonary parenchyma. The mechanism by which the loss of tuberin promotes the development of LAM has yet to be elucidated, although several lines of evidence suggest it is due to the metastasis of tuberin-deficient cells. Here we show that tuberin-null cells become nonadherent and invasive. These nonadherent cells express cleaved forms of ?-catenin. In reporter assays, the ?-catenin products are transcriptionally active and promote MMP7 expression. Invasion by the tuberin-null cells is mediated by MMP7. Examination of LAM tissues shows the expression of cleaved ?-catenin products and MMP7 consistent with a model that tuberin-deficient cells acquire invasive properties through a ?-catenin–dependent mechanism, which may underlie the development of LAM. PMID:20042714

  16. Organ-specific microenvironment modifies diverse functional and phenotypic characteristics of leukemia-associated macrophages in mouse T cell acute lymphoblastic leukemia.

    PubMed

    Chen, Sha-Yan; Yang, Xiao; Feng, Wen-Li; Liao, Jin-Feng; Wang, Li-Na; Feng, Li; Lin, Yong-Min; Ren, Qian; Zheng, Guo-Guang

    2015-03-15

    Tumor-associated macrophages are widely studied in solid tumors. The distribution of macrophages in lymph node samples was found to be associated with the prognosis of lymphoma patients. However, the role of macrophages in leukemia and their functional and phenotypic characteristics in hematopoietic malignancies have not been defined. In this study, we examined the distribution and functional and phenotypic characteristics of macrophages in a Notch1-induced mouse model of T cell acute lymphoblastic leukemia (T-ALL). The distribution of macrophages in bone marrow (BM) and spleen, which are proposed as BM and spleen leukemia-associated macrophages (LAMs), were different during the development of leukemia. LAMs stimulated the proliferation of T-ALL cells and had higher migration activity. RNA-sequencing analysis revealed that gene expression profiles of BM and spleen LAMs showed considerable differences. RT-PCR analysis showed that LAMs expressed both M1- and M2-associated phenotypic genes, but they expressed much lower levels of TGF-?1, VEGF-A, and CSF-1 than did tumor-associated macrophages from B16 melanoma. Furthermore, spleen LAMs more potently stimulated the proliferation of T-ALL cells compared with BM LAMs. Moreover, LAMs could be subdivided into M1-like (CD206(-)) and M2-like (CD206(+)) groups. Both CD206(+) and CD206(-) LAMs stimulated the proliferation of T-ALL cells, although CD206(+) LAMs expressed higher levels of most M1- and M2-associated genes. These results suggested the functional and phenotypic characteristics of LAMs, which were modified by organ specific microenvironments. Our results broaden our knowledge about macrophages in malignant microenvironments from solid tumors to leukemia. PMID:25662994

  17. Lymphangioleiomyomatosis: differential diagnosis and optimal management

    PubMed Central

    Xu, Kai-Feng; Lo, Bee Hong

    2014-01-01

    Lymphangioleiomyomatosis (LAM) is an uncommon disease presented as diffuse thin-walled cystic changes in the lung. The main differential diagnoses include pulmonary Langerhans’ histiocytosis (PLCH), Birt-Hogg-Dubé syndrome (BHD), lymphoid interstitial pneumonia (LIP), and amyloidosis. A combination of clinical, radiological, and pathological approaches as well as genetic testing will clarify the diagnosis in most cases. LAM is a disease almost exclusively in women. Dyspnea, pneumothorax, and hemoptysis are common presentations in LAM patients. LAM is also a lymphatic disorder affecting lymphatic vessels and lymph nodes. Chylothorax, chylous ascites, and lymphangiomyomas are frequently seen. LAM can present sporadically as a single entity or as part of tuberous sclerosis complex (TSC). Angiomyolipoma (AML) is a characteristic extra-pulmonary lesion, either found in association with sporadic or TSC-related LAM. High-risk populations should be screened for LAM, including adult women with TSC and female patients with spontaneous pneumothorax, AMLs in the kidney, and diffuse cystic lung diseases. Definitive diagnosis of LAM is based on a high level of clinical suspicion on presentation supported by pathological findings or by a distinct feature, such as a history of TSC, AMLs in the kidney, chylothorax, or chylous ascites. Vascular endothelial growth factor-D (VEGF-D) in serum is a noninvasive and reliable diagnostic biomarker. In experienced centers, trans-bronchial lung biopsy (TBLB) provides a convenient and safe way to obtain lung specimens for diagnostic purposes. An effective treatment for LAM is now available, namely using a mechanistic target of rapamycin (mTOR) inhibitor such as sirolimus. Efficacy of sirolimus has been confirmed in clinical trials. Research in other molecular-targeted therapies is under investigation. A previously little-known rare disease with no cure is now better understood with regards to its pathogenesis, diagnosis, and management. In this review, current knowledge in diagnosis and differential diagnosis of LAM will be discussed, followed by the discussion of therapy with mTOR inhibitors. PMID:25187723

  18. Progesterone and estradiol synergistically promote the lung metastasis of tuberin-deficient cells in a preclinical model of lymphangioleiomyomatosis.

    PubMed

    Sun, Yang; Zhang, Erik; Lao, Taotao; Pereira, Ana M; Li, Chenggang; Xiong, Li; Morrison, Tasha; Haley, Kathleen J; Zhou, Xiaobo; Yu, Jane J

    2014-10-01

    Lymphangioleiomyomatosis (LAM) is a female-predominant lung disease that can lead to respiratory failure. LAM cells typically have inactivating tuberous sclerosis 2 (TSC2) mutations, leading to mTORC1 hyperactivation. The gender specificity of LAM suggests that female hormones contribute to disease progression. Clinical findings indicate that estradiol exacerbates LAM behaviors and symptoms. Although hormonal therapy with progesterone has been employed, the benefit in LAM improvement has not been achieved. We have previously found that estradiol promotes the survival and lung metastasis of cells lacking tuberin in a preclinical model of LAM. In this study, we hypothesize that progesterone alone or in combination with estradiol promotes metastatic behaviors of TSC2-deficient cells. In cell culture models of TSC2-deficient LAM patient-derived and rat uterine leiomyoma-derived cells, we found that progesterone treatment or progesterone plus estradiol resulted in increased phosphorylation of Protein Kinase B (Akt) and Extracellular signal-regulated kinases1/2 (ERK1/2), induced the proliferation, and enhanced the migration and invasiveness. In addition, treatment of progesterone plus estradiol synergistically decreased the levels of reactive oxygen species and enhanced cell survival under oxidative stress. In a murine model of LAM, treatment of progesterone plus estradiol promoted the growth of xenograft tumors; however, progesterone treatment did not affect the development of xenograft tumors of Tsc2-deficient cells. Importantly, treatment of progesterone plus estradiol resulted in alteration of lung morphology and significantly increased the number of lung micrometastases of Tsc2-deficient cells compared with estradiol treatment alone. Collectively, these data indicate that progesterone increases the metastatic potential of Tsc2-deficient LAM patient-derived cells in vitro and lung metastasis in vivo. Thus, targeting progesterone-mediated signaling events may have therapeutic benefit for LAM and possibly other hormonally dependent cancers. PMID:25069840

  19. Medicinal plants of Chile: evaluation of their anti-Trypanosoma cruzi activity.

    PubMed

    Muñoz, Orlando M; Maya, Juan D; Ferreira, Jorge; Christen, Philippe; San Martin, José; López-Muñoz, Rodrigo; Morello, Antonio; Kemmerling, Ulrike

    2013-01-01

    The extracts of several plants of Central Chile exhibited anti-Trypanosoma cruzi trypomastigotes activity. Most active extracts were those obtained from Podanthus ovatifolius, Berberis microphylla, Kageneckia oblonga, and Drimys winteri. The active extract of Drimys winteri (IC50 51.2 microg/mL) was purified and three drimane sesquiterpenes were obtained: polygodial, drimenol, and isodrimenin. Isodrimenin and drimenol were found to be active against the trypomastigote form of T. cruzi with IC50 values of 27.9 and 25.1 microM, respectively. PMID:23923616

  20. Evaluation of tick-host-landscape interactions using spatial analysis 

    E-print Network

    Bush, Beau R

    1997-01-01

    Copita Research Area, Jim Wells Co. TX 29 12 Percentage distribution of brush polygons in 1 m increments within the range of 10-31 m on the West Pasture for the La Copita Research Area, Jim Wells Co. , TX. 30 Page 13 Percentage of the East Pasture... persimmon (Diospyros texatta), blackbrush acacia (Acacia rigidtda), Condalia, whitebrush (Aloysia lycioides), and agarita (Berberis trifoliata), in addition to many other less dominant species indigenous to that area. A herd of 16 cross-bred cows were...

  1. STAG UK Newsletter Issue 18 

    E-print Network

    1976-01-01

    ~S.l\\. ***************** 1\\dri8n Meson tells me thet 'Berbery COElst' WEIS tsken off the Irish Television Stetion REldio TelefisEireen Elfter'oniy about nine or so epi13 odes. On the other h2nd, Nikki White tells me th8t {t is still being shown..., but these animals 8re at a disadvant8ge in other parts of their structur,8.. The elephant' s stze and welg,ht ere certainly no asset for building a great,civilis8tion. ~nd would not octopi heve to becom~ land creatures before developing a civilised cul ture...

  2. Lymphatics in lymphangioleiomyomatosis and idiopathic pulmonary fibrosis

    PubMed Central

    Glasgow, Connie G.; El-Chemaly, Souheil; Moss, Joel

    2013-01-01

    The primary function of the lymphatic system is absorbing and transporting macromolecules and immune cells to the general circulation, thereby regulating fluid, nutrient absorption and immune cell trafficking. Lymphangiogenesis plays an important role in tissue inflammation and tumour cell dissemination. Lymphatic involvement is seen in lymphangioleiomyomatosis (LAM) and idiopathic pulmonary fibrosis (IPF). LAM, a disease primarily affecting females, involves the lung (cystic destruction), kidney (angiomyolipoma) and axial lymphatics (adenopathy and lymphangioleiomyoma). LAM occurs sporadically or in association with tuberous sclerosis complex (TSC). Cystic lung destruction results from proliferation of LAM cells, which are abnormal smooth muscle-like cells with mutations in the TSC1 or TSC2 gene. Lymphatic abnormalities arise from infiltration of LAM cells into the lymphatic wall, leading to damage or obstruction of lymphatic vessels. Benign appearing LAM cells possess metastatic properties and are found in the blood and other body fluids. IPF is a progressive lung disease resulting from fibroblast proliferation and collagen deposition. Lymphangiogenesis is associated with pulmonary destruction and disease severity. A macrophage subset isolated from IPF bronchoalveolar lavage fluid (BALF) express lymphatic endothelial cell markers in vitro, in contrast to the same macrophage subset from normal BALF. Herein, we review lymphatic involvement in LAM and IPF. PMID:22941884

  3. Anti-EGFR Antibody Reduces Lung Nodules by Inhibition of EGFR-Pathway in a Model of Lymphangioleiomyomatosis

    PubMed Central

    Ancona, Silvia; Orpianesi, Emanuela; Di Giulio, Anna Maria; Gorio, Alfredo

    2015-01-01

    EGFR belongs to the HER/ErbB family of tyrosine kinase receptors and its activation in cancer cells has been linked with increased proliferation, angiogenesis, and metastasis. Lymphangioleiomyomatosis (LAM) is a rare, low-grade neoplasm that occurs sporadically or in association with tuberous sclerosis complex (TSC), a genetic, multisystem disorder characterized by hamartomas in several organs. From chylous of a LAM/TSC patient, we previously isolated smooth muscle-like LAM/TSC cells whose proliferation depends on EGF and monoclonal anti-EGFR antibodies reduced proliferation and caused cell death. We demonstrated that the dependency from EGF was caused by the absence of tuberin. To study the role of EGFR pathway in vivo, we developed a mouse model by administration of LAM/TSC cells to female nude mice. LAM/TSC cells caused pulmonary airspace enlargement and, after 30 weeks, nodule formation which express EGFR. Anti-EGFR antibody decreased the number and dimension of lung nodules likely for the inhibition of Erk and S6 signaling, reversed the pulmonary alterations, and reduced lymphatic and blood vessels. Moreover, in pulmonary nodules anti-EGFR antibody reduced the positivity to estrogen and progesterone receptors which enhance survival of LAM cells and Snail expression. These results suggest that the inhibition of EGFR signalling has a potential in treatment of LAM/TSC lung alterations. PMID:25699271

  4. Laser assisted microdissection, an efficient technique to understand tissue specific gene expression patterns and functional genomics in plants.

    PubMed

    Gautam, Vibhav; Sarkar, Ananda K

    2015-04-01

    Laser assisted microdissection (LAM) is an advanced technology used to perform tissue or cell-specific expression profiling of genes and proteins, owing to its ability to isolate the desired tissue or cell type from a heterogeneous population. Due to the specificity and high efficiency acquired during its pioneering use in medical science, the LAM technique has quickly been adopted for use in many biological researches. Today, it has become a potent tool to address a wide range of questions in diverse field of plant biology. Beginning with comparative transcriptome analysis of different tissues such as reproductive parts, meristems, lateral organs, roots etc., LAM has also been extensively used in plant-pathogen interaction studies, proteomics, and metabolomics. In combination with next generation sequencing and proteomics analysis, LAM has opened up promising opportunities in the area of large scale functional studies in plants. Ever since the advent of this technique, significant improvements have been achieved in term of its instrumentation and method, which has made LAM a more efficient tool applicable in wider research areas. Here, we discuss the advancement of LAM technique with special emphasis on its methodology and highlight its scope in modern research areas of plant biology. Although we put emphasis on use of LAM in transcriptome studies, which is mostly used, we also discuss its recent application and scope in proteome and metabolome studies. PMID:25403993

  5. Stranger in a strange land: Ibero-American strain of Mycobacterium tuberculosis in Tibet, China.

    PubMed

    Mokrousov, Igor; Jiao, Wei-Wei; Wan, Kanglin; Shen, Adong

    2014-08-01

    The Latin-American-Mediterranean (LAM) family is among the largest, diverse and intriguing within Mycobacterium tuberculosis. Widespread in some world regions, as justly reflected by its name, LAM remains in the shadow of the Beijing and East-African Indian families in East Asia. Here, we applied evolutionarily robust markers to the spoligotyped collection of 699 M. tuberculosis clinical isolates recovered from tuberculosis (TB) patients in different provinces across China between 2005 and 2013. As a result, only two isolates were assigned to LAM, hence we conclude that this family has a negligible impact on TB transmission in China. Interestingly, these two isolates represented two grand sublineages within LAM: RD115 and RD-Rio/RD174. Our results highlight the great caution to be taken when interpreting spoligotyping profiles with large deleted blocks. Here, three geographically distant Chinese isolates of spoligotype SIT803 were excluded from LAM. This is in clear contrast with described SIT803 isolates of the LAM family in Russia and Georgia (Mokrousov et al., 2014; Niemann et al., 2010). Interestingly, a drug resistant isolate of the Ibero-American LAM RD-Rio sublineage was recovered from TB patient of Tibetan ethnicity, a resident in the Lhasa district in Tibet. An additional in-depth analysis by whole genome sequencing might help to understand the evolutionary history of this isolate and its possible route to the rural setting of the Tibetan highlands. PMID:24956435

  6. A pilot randomized controlled trial of dual-plasmid HBV DNA vaccine mediated by in vivo electroporation in chronic hepatitis B patients under lamivudine chemotherapy.

    PubMed

    Yang, F-Q; Yu, Y-Y; Wang, G-Q; Chen, J; Li, J-H; Li, Y-Q; Rao, G-R; Mo, G-Y; Luo, X-R; Chen, G-M

    2012-08-01

    A DNA vaccine against the hepatitis B virus (HBV), enhanced by IL-2/IFN-? fusion protein expression from a plasmid construct and mediated by in vivo electroporation, was evaluated in a total of 39 HBeAg-positive patients with chronic hepatitis B (CHB). The six of 39 patients with a serum alanine aminotransferase (ALT) value of 1-2 times upper limit of normal (ULN) were assigned to the open-label arm (Group01) receiving vaccine monotherapy; the remaining 33 patients with an ALT of more than two times ULN were enroled to the randomized and controlled arm (Group02) receiving lamivudine (LAM) monotherapy (LAM+placebo) or combined therapy (LAM+DNA vaccine) in 1:2 ratio. In Group01, a significant elevation of HBV-specific IFN-?-secreting T-cell counts in comparison with baseline was observed. In Group02, the proportion of patients with HBV DNA suppression was higher with LAM+DNA vaccine than with LAM monotherapy at each visit time point after the final injection of DNA vaccine at week 36, revealing a significant difference between the two groups (P = 0.03) at week 60. The incidence of dual-site mutations of rtM204/I/S+rtL180M was significantly lower (P = 0.03) with an identified lower virological breakthrough (VBT) rate (P = 0.03) in patients receiving LAM+DNA vaccine than LAM monotherapy, accompanied with a significant higher positive T-cell response rate in patients receiving LAM+DNA vaccine (P = 0.03). In conclusion, this study provides evidence that HBV DNA vaccination is safe and immunologically effective, and that the HBV-specific T-cell responses induced by DNA vaccination under LAM chemotherapy showed a correlation with the suppression of viral replication in patients with CHB. PMID:22762143

  7. Incidental Pelvic and Para-aortic Lymph Node Lymphangioleiomyomatosis Detected During Surgical Staging of Pelvic Cancer in Women Without Symptomatic Pulmonary Lymphangioleiomyomatosis or Tuberous Sclerosis Complex.

    PubMed

    Rabban, Joseph T; Firetag, Brandie; Sangoi, Ankur R; Post, Miriam D; Zaloudek, Charles J

    2015-08-01

    Extrapulmonary lymphangioleiomyomatosis (LAM) is a rare neoplasm of spindle cells exhibiting melanocytic and myoid differentiation that arises as a mass in the mediastinum, retroperitoneum, uterine wall, and/or intraperitoneal lymph nodes. Many patients also have pulmonary LAM, tuberous sclerosis complex (TSC), and/or other neoplasms of the perivascular epithelioid cell tumor family. This study reports 26 patients with clinically occult LAM involving pelvic/para-aortic lymph nodes removed from women undergoing surgical staging of a uterine (17), ovarian (5), cervical (3), or urinary bladder (1) neoplasm. None of the patients exhibited symptoms of pulmonary LAM, and the median patient age (56 y) was older than what would be expected for patients presenting with pulmonary LAM. Only 2/26 patients had TSC. Four patients also had uterine LAM. One of these 4 had uterine perivascular epithelioid cell tumor, and 1 had vaginal angiomyolipoma. In all 26 patients the lymph node LAM was grossly occult, measured 3.5 mm on average (1 to 19 mm), and involved either a single lymph node (12/26) or multiple lymph nodes (14/26). HMB45 was positive in 24/25 cases, mostly in a focal or patchy distribution. Other melanocytic markers included MiTF (12/14) and MelanA (2/12). Myoid markers included smooth muscle actin (23/23) and desmin (15/16), mostly in a diffuse distribution. Estrogen receptor was positive in all cases tested, as was D240 expression in the lymphatic endothelium lining the spindle cell bundles. Concurrent findings in the involved lymph nodes included metastatic carcinoma (3/26), endosalpingiosis (3/26), and reactive lymphoid hyperplasia (13/26). This study demonstrates that clinically occult lymph node LAM can be detected during surgical staging of pelvic cancer and is not commonly associated with pulmonary LAM or TSC, although these patients should still be formally evaluated for both of these diseases. PMID:25786086

  8. Mycobacterium tuberculosis Latin American-Mediterranean Family and Its Sublineages in the Light of Robust Evolutionary Markers

    PubMed Central

    Vyazovaya, Anna; Narvskaya, Olga

    2014-01-01

    Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of the LAM family and its major sublineages circulating in Russia and neighboring countries. A total of 250 M. tuberculosis isolates were confirmed to belong to the LAM family based on the analysis of the LAM-specific single-nucleotide polymorphisms (SNPs) in the Rv3062 and Rv0129c genes. At this stage, the family status was rectified for 121 isolates misleadingly assigned by CRISPR spoligotyping to non-LAM families (T1- or T5-RUS1). Consequently, the reestimated LAM prevalence rate increased 2-fold in Russia and Kazakhstan and 4-fold in Belarus. The majority (91.8 to 98.7%) of the LAM isolates from all three countries belonged to the LAM-RUS sublineage. In contrast, the Ibero-American LAM RD-Rio sublineage was identified in only 7 Russian isolates. Taken together, our findings and further analyses suggest a monophyletic origin of LAM-RUS: at a historically distant time, in Russia, in a small founding bacterial/human population. Its dissemination pattern and high prevalence rate in Northern Eurasia may indicate a long-term coexistence of the LAM-RUS sublineage and local human populations hypothetically leading to coadaptation and reduced pathogenicity of the relatively more ancient clones, such as spoligotype international type 254 (SIT254), compared to the more recent SIT252 and SIT266 clones. In contrast, rare LAM RD-Rio isolates were likely brought to Russia through occasional human contact. The spread of RD-Rio strains is not as global as commonly claimed and is determined largely by human migration flows (rather than by pathobiological properties of these strains). Consequently, a host population factor appears to play a major role in shaping the in situ dissemination pattern of the imported strains in an autochthonous population. PMID:24584500

  9. Enantiomeric Free Radicals and Enzymatic Control of Stereochemistry in a Radical Mechanism: the Case of Lysine 2,3-aminomutases †

    PubMed Central

    Behshad, E.; Ruzicka, F.J; Mansoorabadi, S.; Chen, D.; Reed, G.H; Frey, P.A.

    2008-01-01

    The product of yjeK in Escherichia coli is a homolog of lysine 2,3-aminomutase (LAM) from Clostridium subterminale SB4, and both enzymes catalyze the isomerization of (S)- but not (R)-?-lysine by radical mechanisms. The turnover number for LAM from E. coli is 5.0 min?1, 0.1% of the value for clostridial LAM. The reaction of E. coli LAM with (S)-?-[3,3,4,4,5,5,6,6- 2H8]lysine proceeds with a kinetic isotope effect of kH/kD = 1.4, suggesting that hydrogen transfer is not rate limiting. The product of the E. coli enzyme is (R)-?-lysine, the enantiomer of the clostridial product. ?-Lysine-related radicals are observed in the reactions of both enzymes by electron paramagnetic resonance (EPR). The radical in the reaction of clostridial LAM has the (S)- configuration, whereas that in the reaction of E. coli LAM has the (R)-configuration. Moreover, the conformations of the ?-lysine-related radicals at the active sites of E. coli and clostridial LAM are different. The nuclear hyperfine splitting between the C3-hydrogen and the unpaired electron at C2 shows the dihedral angle to be 6°, unlike the 77° reported for the analogous radical bound to the clostridial enzyme. Reaction of (S)-4-thialysine produces a substrate-related radical in the steady state of E. coli LAM, as in the action of the clostridial enzyme. While (S)-?-lysine is not a substrate for E. coli LAM, it undergoes hydrogen abstraction to form an (S)-?-lysine-related radical with the same stereochemistry of hydrogen transfer from C2 of (S)-?-lysine to the 5?-deoxyadenosyl radical as in the action of the clostridial enzyme. The resulting ?-lysyl radical has a different conformation than that at the active site of clostridial LAM. All evidence indicates that the opposite stereochemistry displayed by the E. coli LAM is determined by the conformation of the lysine side chain in the active site. Stereochemical models for the actions of LAM from C. subterminale and E. coli are presented. PMID:17042480

  10. Safety and efficacy of lamivudine after radiofrequency ablation in patients with hepatitis B virus-related hepatocellular carcinoma

    Microsoft Academic Search

    Hideo Yoshida; Haruhiko Yoshida; Eriko Goto; Takahisa Sato; Takamasa Ohki; Ryota Masuzaki; Ryosuke Tateishi; Tadashi Goto; Shuichiro Shiina; Takao Kawabe; Masao Omata

    2008-01-01

    Background  Antiviral treatments for hepatitis B virus (HBV) are not established in patients with HBV-related hepatocellular carcinoma\\u000a (HCC).\\u000a \\u000a \\u000a \\u000a Aim  To investigate the safety and efficacy of lamivudine (LAM) in patients with HBV-related HCC who were treated with radiofrequency\\u000a ablation (RFA).\\u000a \\u000a \\u000a \\u000a Methods  RFA-treated patients with HBV-related HCC were retrospectively divided into those who received LAM (LAM group) and those who\\u000a did not (nontreatment group).

  11. Mycobacterium tuberculosis Latin American-Mediterranean family and its sublineages in the light of robust evolutionary markers.

    PubMed

    Mokrousov, Igor; Vyazovaya, Anna; Narvskaya, Olga

    2014-05-01

    Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of the LAM family and its major sublineages circulating in Russia and neighboring countries. A total of 250 M. tuberculosis isolates were confirmed to belong to the LAM family based on the analysis of the LAM-specific single-nucleotide polymorphisms (SNPs) in the Rv3062 and Rv0129c genes. At this stage, the family status was rectified for 121 isolates misleadingly assigned by CRISPR spoligotyping to non-LAM families (T1- or T5-RUS1). Consequently, the reestimated LAM prevalence rate increased 2-fold in Russia and Kazakhstan and 4-fold in Belarus. The majority (91.8 to 98.7%) of the LAM isolates from all three countries belonged to the LAM-RUS sublineage. In contrast, the Ibero-American LAM RD-Rio sublineage was identified in only 7 Russian isolates. Taken together, our findings and further analyses suggest a monophyletic origin of LAM-RUS: at a historically distant time, in Russia, in a small founding bacterial/human population. Its dissemination pattern and high prevalence rate in Northern Eurasia may indicate a long-term coexistence of the LAM-RUS sublineage and local human populations hypothetically leading to coadaptation and reduced pathogenicity of the relatively more ancient clones, such as spoligotype international type 254 (SIT254), compared to the more recent SIT252 and SIT266 clones. In contrast, rare LAM RD-Rio isolates were likely brought to Russia through occasional human contact. The spread of RD-Rio strains is not as global as commonly claimed and is determined largely by human migration flows (rather than by pathobiological properties of these strains). Consequently, a host population factor appears to play a major role in shaping the in situ dissemination pattern of the imported strains in an autochthonous population. PMID:24584500

  12. 12-04-19 07:09Une tudiante contribue l'amlioration de la sant du canal Rideau en t...t | Gazette de l'Universit d'Ottawa Branche sur la vie de notre campus Page 1 sur 3http://www.gazette.uottawa.ca/fr/2012/04/une-etudiante-contribue-a-lam...tudiant-le-n

    E-print Network

    Blouin-Demers, Gabriel

    12-04-19 07:09Une étudiante contribue à l'amélioration de la santé du canal Rideau en ét-de-mercure-present-dans-les-tortues-qui-y-habitent/ Une étudiante contribue à l'amélioration de la santé du canal Rideau en étudiant le niveau de mercure://www.gazette.uottawa.ca/wp-content/uploads/2012/04/Julie.jpg) Julie Châteauvert Le canal Rideau, site du patrimoine mondial de l'UNESCO, fait la

  13. Ultra-performance liquid chromatography tandem mass spectrometry combined with automated MetaboLynx analysis approach to screen the bioactive components and their metabolites in Wen-Xin-Formula.

    PubMed

    Cao, Hongxin; Zhang, Aihua; Zhang, Fang-mei; Wang, Qin-qin; Zhang, He; Song, Yan-hua; Zhou, Ying; Sun, Hui; Yan, Guang-li; Han, Ying; Wang, Xijun

    2014-12-01

    Wen-Xin-Formula (WXF), a famous traditional prescription, has been widely used to treat myocardial ischemia syndrome for thousands of years. However, the constituents absorbed into blood after oral administration of WXF remain unknown. Here, an integrative ultra performance liquid chromatography coupled with electrospray ionization/quadrupole-time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS) combined with the MetaboLynx approach was established to investigate the absorbed constituents in rats after oral administration of WXF. A hyphenated electrospray ionization and quadrupole-time-of-flight analyzer was used for the determination of accurate mass of the molecule and fragment ions. With this rapid and automated analysis method, a total of 32 peaks were tentatively characterized in vivo based on MS and MS/MS data and comparison with available databasess, 26 of which were parent components and six metabolites. These components mainly were ginsenosides, paeoniflorin, galloyl glucose, berberis alkaloids, phenolic, phenolic glycosides and unsaturated fatty acids, glucuronide products of original berberis alkaloids. The present study demonstrates that integrative UPLC-ESI-Q-TOF-MS technique and MetaboLynx data processing method were successfully applied for the rapid discovery of potentially bioactive components and metabolites from WXF, and proved that the established method could help to explore the effective substances for further research into WXF. PMID:24853889

  14. Survival of Bundleless Hair Cells and Subsequent Bundle Replacement in the Bullfrog's Saccule

    E-print Network

    of the bullfrog saccule a combination of time-lapse video mi- croscopy, two-photon microscopy, electron microscopy deficits (Lam- bert, 1994). That is not the case, however, for non- mammalian vertebrates. In fish

  15. UBC Social Ecological Economic Development Studies (SEEDS) Student Report Education Programs in the LFS Orchard Garden and Agora Caf

    E-print Network

    in the LFS Orchard Garden and Agora Café Elaine Yi-Ling Chen Kristi Hatakka Wennie Lam Janette Liang Sara of a project/report". #12;Scenario 4: Education Programs in the LFS Orchard Garden and Agora Café Land Food

  16. In: Biology, Evolution and Conservation of River Dolphins... ISBN 978-60876-633-8 Editors: Manuel Ruiz-Garcia and Joseph Shostell 2010 Nova Science Publishers, Inc.

    E-print Network

    Solé-Cava, Antonio M.

    as in the Amazon River basin (Figure 1). Marine Sotalia are found from Honduras to the state of Santa Catarina and Colombia) and "lam" (Nicaragua). The distribution of riverine Sotalia comprises most of the Amazon River

  17. Draft genome sequence of “Candidatus Liberibacter americanus” bacterium associated with citrus huanglongbing in Brazil

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The genus of‘Candidatus Liberibacter’ belongs to Gram-negative, a-proteobacteria. There are four pathogenic Liberibacter species in this genus, ‘Candidatus Liberibacter asiaticus’ (Las), ‘Candidatus Liberibacter africanus’ (Laf) and ‘Candidatus Liberibacter americanus’ (Lam), which are associated wi...

  18. Rapamune Approved for Rare Lung Disease

    MedlinePLUS

    ... a rare, progressive lung disease that mostly affects women of childbearing age. Lymphangioleiomyomatosis (LAM) is characterized by the unusual growth of smooth-muscle cells among lung tissue. This can block normal ...

  19. The foxtail (Setaria) species-group

    Microsoft Academic Search

    Jack Dekker

    2003-01-01

    5 yellow foxtail, Setaria pumila (Poir) Roem. & Schult. 5 Setaria glauca (L.) Beauv. SETLU; giant foxtail, Setaria faberi Herrm. SETFA; bristly foxtail, Setaria verticillata (L.) Beauv. SETVE; knotroot foxtail, Setaria geniculata (Lam.) Beauv. SETGE.

  20. A campus of The California State University Office of the Academic Senate One Washington Square San Jose, California 95192-0024 408-924-2440 Fax: 408-924-2451

    E-print Network

    Gleixner, Stacy

    , and social well-being of research subjects is a moral, ethical, and legal imperative, and WHEREAS Miura Absent: Ruth Wilson, Caroline Fee, Jennifer Lam, Chang Choo Vote: 6-0-0 Financial Impact: None

  1. Steric Hindrance, Electronic Communication, and Energy Transfer in the Photo-and Electroluminescence Processes of Aggregation-Induced Emission Luminogens

    E-print Network

    - and Electroluminescence Processes of Aggregation-Induced Emission Luminogens Zujin Zhao, Shuming Chen, Jacky W. Y. Lam as excellent emitters in electroluminescence (EL) devices, with maximum luminance up to 12560 cd/m2 . The EL

  2. Facelifts May Make Women Seem More Likable, Study Suggests

    MedlinePLUS

    ... perceive you from the outside," Lam said. Timothy Smith, a professor of psychology at Brigham Young University ... is really just the absence of remarkable features," Smith said. "Then you line that up with a ...

  3. The iron nutrition of tropical foliage plants

    E-print Network

    Lang, Harvey Joe

    1986-01-01

    elegans Liebm. ' Belle ', Dracaena margi nate Lam. , Di ef fenttachi a maculate Lodd. ' Camille ', Di ef fenhachi a maculate Lodd. ' Compacta ', Epi premnum aureum Engl . , Philodendron scandens oxycardi um Schott . , Araucari a heterophyl la R. Br...

  4. STANFORD HOSPITAL AND CLINICS ICU ON-CALL SCHEDULE

    E-print Network

    Ford, James

    (GONDA/ARBO) additional clinical coverage HSU LAM REID BRIDGE ZIRLINGER (GONDA/ARBO) additional clinical. Jeremy Gonda 14165 Dr. Tatyana Travinka Blue 22159 Dr. Cristina Vazquez Dr. Feras Khan 14190 Dr. Shirin

  5. Examining Non-Linear Relationships between Human Resource Practices and Manufacturing Performance

    E-print Network

    Chadwick, Clint

    2007-07-01

    1994), petro-chemicals (Wright et al. 1999), apparel (Dunlop and Weil 1996), general manufac turing (Lee and Miller 1999; Lam and White 1998), and heavy manufacturing (Dean and Snell 1996; Youndt et al. 1996). Variables The means, standard...

  6. New Phytologist (2002) 155: 373380 www.newphytologist.com 373 Blackwell Science, Ltd

    E-print Network

    Britto, Dev T.

    2002-01-01

    the plastidic isoform of glutamine synthetase (Lam et al., 1996), and liberated mainly through the serine synthase reaction of the photorespiratory nitrogen cycle (Givan et al., 1988; Leegood et al., 1995), amino

  7. Elliptic Faulhaber polynomials and Lamé densities of states

    E-print Network

    M. -P. Grosset; A. P. Veselov

    2005-08-31

    A generalisation of the Faulhaber polynomials and Bernoulli numbers related to elliptic curves is introduced and investigated. This is applied to compute the density of states for the classical Lam\\'e operators.

  8. Soil carbon and nitrogen dynamics as affected by inputs of dairy manure and poultry litter 

    E-print Network

    Haney, Richard Lee

    1997-01-01

    was a 2x4 factorial within a randomized complete block replicated four times. A dryland field plot experiment with coastal bermudagrass and coastal bermudagrass interseeded with ryegrass (Loli um multi/lorum Lam. ) was established at Overton, Texas...

  9. Forage production and animal performance from Kenhy tall fescue 

    E-print Network

    Dorsett, D. J

    1976-01-01

    an annual ryegrass (Loli um multiflorum Lam. ) and tall fescue (Festuca arundinacea Schreb. ) cross. Since Kenhy tall fescue has not been evaluated in Texas, a study was conducted under local conditions to evaluate its perfor- mance. The objectives...

  10. Vrije Universiteit Brussel Pleinlaan 2

    E-print Network

    Steels, Luc

    jg.15 · nR.2 mei - jUni 2012 lam gods in 100 miljard pixels Podiumplaats voor Uz Brussel Professoren internationale team van wetenschappers uit Brussel en de Verenigde Staten, bestaande uit Vincent ginis, Philippe

  11. nEmesis: Which Restaurants Should You Avoid Today? Adam Sadilek

    E-print Network

    Kautz, Henry

    University of Rochester Rochester, NY kautz@cs.rochester.edu Vincent Silenzio University of Rochester learning has demonstrated that online social me- dia enable novel surveillance and modeling tools (Lam- pos

  12. BOSTON UNIVERSITY GRADUATE SCHOOL OF ARTS AND SCIENCES

    E-print Network

    Al Hanbali, Ahmad

    thanks to Melina, Marina, Rits, Vincent, and Dave for help and support throughout these years. Also, was developed by Lam and Goussis, and it is a method formulated in the tangent bundle of the state space

  13. IOP PUBLISHING PLASMA SOURCES SCIENCE AND TECHNOLOGY Plasma Sources Sci. Technol. 20 (2011) 055001 (9pp) doi:10.1088/0963-0252/20/5/055001

    E-print Network

    Economou, Demetre J.

    2011-01-01

    with synchronous bias on a boundary electrode Hyungjoo Shin, Weiye Zhu, Lin Xu1 , Vincent M Donnelly and Demetre J selectivity of SiO2 over Si. 1 Currently with Lam Research, Fremont, CA, USA. If current trends continue

  14. Thse de Doctorat prsente pour obtenir le titre de Docteur de l'Universit Paris-Est

    E-print Network

    Paris-Sud XI, Université de

    et directeur du Laboratoire de Mécanique (LaM) à Marne-la-Vallée, qui a accepté de codiriger ma thèse remercie Yacine Khidas et Vincent Langlois pour les discussions scientifiques que j'ai pu avoir avec eux

  15. Iron phosphate precipitation by epilithic microbial biofilms in Arctic Canada K.O. KONHAUSERAND W.S. FYFE

    E-print Network

    Konhauser, Kurt

    . SCHULTZE-LAM AND F.G. FERRIS Department of Geology, University of Toronto, 22 Russell Street, Toronto, ON M in extreme polar surface environments (see Vincent 1988 for a review). Their uniqueness arises from

  16. UNIVERSIT MONTESQUIEU BORDEAUX IV SCIENCES PO BORDEAUX

    E-print Network

    Paris-Sud XI, Université de

    BORDEAUX LAM ­ Les Afriques dans le Monde (UMR 5115 du CNRS) LA GOUVERNANCE SANS ETAT : UNE ETUDE DE CAS Studies, Oslo (Rapporteur) M. FOUCHER Vincent, Chargé de recherche au CNRS, Les Afriques dans le Monde

  17. Pour obtenir le grade de DOCTEUR DE L'UNIVERSIT DE GRENOBLE

    E-print Network

    Boyer, Edmond

    , surtout dans le cadre de l'association GipsaDoc : Oumayma, Andra, Lizeth, Amine, Lam, Jou- mana, Ignacio, Jennifer, Emilie, Vincent, Chloe, Yo, Patricia. Un grand merci à ma chère collègue de bureau Oumayma, qui m

  18. Universit Montpellier II --Sciences et Techniques du Languedoc --

    E-print Network

    Paris-Sud XI, Université de

    Vincent Huon. J'adresse ma re- connaissance à Jean-Michel Muracciole et Bruno Koobus pour toutes ces Grégori du LSPM et Guillaume Thoquenne du CETIM. Je souhaite de plus une bonne continuation à Ngoc-Lam

  19. pour obtenir le grade de l'Institut des Sciences et Industries du Vivant et de l'Environnement

    E-print Network

    Paris-Sud XI, Université de

    David Makowski pour ses conseils avisés sur le bayésien, à Matiyiendu Lam- boni et Hervé Monod pour nos biocarburants. Merci à Mickael Schulz et Vincent Prieur pour nos collaborations dans le cadre du projet N

  20. UNIVERSITE MONTESQUIEU BORDEAUX IV ECOLE DOCTORALE DE SCIENCE POLITIQUE (ED 208)

    E-print Network

    Paris-Sud XI, Université de

    'ont apportée. A Bordeaux, mes remerciements vont à l'ensemble de l'équipe du LAM, en particulier à Messieurs Christian COULON et Michel CAHEN pour leur soutien et leur amitié. Je pense aussi à Céline THIRIOT, Vincent

  1. DATWYLER & WEIBLEN. American Journal of Botany 91(5): 767-777. 2004. Data Supplement Page 1 SHANNON DATWYLER AND GEORGE D. WEIBLEN. 2004. On the origin of the fig: Phylogenetic relationships of Moraceae from ndhF

    E-print Network

    Weiblen, George D

    Broussonetia papyrifera (L.) Vent. Weiblen 1176 cult;, Beal Bot. Gard. AY289269 Fatuoa pilosa Gaudich. Vincent289286 Artocarpus camansi Blanco Weiblen 1237 New Guinea AY289288 Artocarpus heterophyllus Lam. Weiblen

  2. UNIVERSIT PARIS-SUD COLE DOCTORALE : Ondes et Matire

    E-print Network

    Paris-Sud XI, Université de

    en ressources humaines par moments. Je remercie Philippe Bouyer et Vincent Josse pour ce projet Hambrecht, qui m'ont accueillie avec enthousiasme, puis le groupe a été rejoint par Tung- Lam Dao, Thomas

  3. Author's personal copy Brief article

    E-print Network

    Love, Bradley C.

    learning q W. Todd Maddox a,*, Bradley C. Love a , Brian D. Glass a , J. Vincent Filoteo b a Department, Gitelman, Parrish, & Mesu- lam, 2003; Seger & Cincotta, 2005, 2006). Two neural circuits that subserve

  4. Annual Report: 0618210 Page 1 of 13

    E-print Network

    studies Name: Gutshick, Vincent Worked for more than 160 Hours: Yes Contribution to Project: Co-PI working student working with Throop on decomposition studies Name: Kuok Choy, Lam Worked for more than 160 Hours

  5. All projects in the Center involve multiple Co-PIs, with the majority of the projects involving Co-PIs from

    E-print Network

    Eustice, Ryan

    . Jeffrey Marks, Lam Research Inc. · Dr. Tim Sommerer, General Electric · Dr. Nick Fuller, IBM · Dr. Mark, University of Michigan · Vladimir Demidov, West Virginia University · Vincent Donnelly, University of Houston

  6. A Game Semantics Foundation for Logic Programming (Extended Abstract)

    E-print Network

    Loddo, Jean-Vincent

    A Game Semantics Foundation for Logic Programming (Extended Abstract) Roberto Di Cosmo, Jean­Vincent is a very active research subject in computer science. After the preliminary works of Lamarche [Lam95

  7. COLE DOCTORALE DE PHYSIQUE ET SCIENCES DE LA MATIRE UNIVERSITE DE PROVENCE -AIX-MARSEILLE I

    E-print Network

    Paris-Sud XI, Université de

    des équipes Exoplanètes du LAM et de l'IAP se fit très naturellement, et ses membres m'ont rapide remercier Thomas Fenouillet, Vincent Herrero, Jean-Charles Meunier, Christian Surace et les membres du SCI

  8. THESE DE DOCTORAT DE L'UNIVERSIT PIERRE ET MARIE CURIE

    E-print Network

    Paris-Sud XI, Université de

    plaisir d'encadrer deux stagiaires, Nicolas Fourdrin et Vincent François, dont les fruits des travaux se'équipe LAM ; aussi, j'aimerais en remercier tous les membres pour leur contribution à rendre ce laboratoire

  9. prsente L'UFR DES SCIENCES ET TECHNIQUES

    E-print Network

    Boyer, Edmond

    vou- drais tout d'abord remercier Monsieur Bernard Cretin, Monsieur Vincent Laude, Monsieur Bernard contribuent par leur bonne humeur à l'am- biance agréable que j'ai apprécié pendant trois ans. La liste n

  10. Master recherche de Bioinformatique Universite de Rennes 1

    E-print Network

    Richer, Jean-Michel

    ´ecieuse en programmation et leur humour au quotidien. Olivier Cantin, Herv´e Deleau, Vincent Derrien, Adrien Goeffon, Olivier H^u, Tony Lam- bert, Frederic Lardeux, Thomas Raimbault, Antoine Robin pour leur accueil

  11. A Game Semantics Foundation for Logic Programming (Extended Abstract)

    E-print Network

    Loddo, Jean-Vincent

    A Game Semantics Foundation for Logic Programming (Extended Abstract) Roberto Di Cosmo # Jean­Vincent the preliminary works of Lamarche [Lam95], Blass [Bla92] and Joyal [Joy95] in the early 90s, the works of Abramsky

  12. POSTER PRESENTATION Open Access Highlighting the genetic and epidemiologic

    E-print Network

    Boyer, Edmond

    : Bahamas, Barbados, Belize, Dominica, Guyana, Jamaica, St. Kitts and Nevis, St. Lucia, St. Vincent.0%), Latin American and Mediterranean (LAM, 10.4%), the X clade (8.3%), Haarlem (5.8%), and Beijing (3

  13. Reflection Analysis for Java Benjamin Livshits John Whaley

    E-print Network

    Livshits, Ben

    Reflection Analysis for Java Benjamin Livshits John Whaley Monica S. Lam Computer Science . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2 Paper Organization . . . . . . . . . . . . . . . . . . . . . . . . 10 2 Overview of Reflection in Java 11 2.1 Reflection APIs in Java . . . . . . . . . . . . . . . . . . . . . . 11 2.1.1 Obtaining

  14. Tenofovir disoproxil fumarate is superior to lamivudine plus adefovir in lamivudine-resistant chronic hepatitis B patients

    PubMed Central

    Yang, Dan-Hong; Xie, Yuan-Jun; Zhao, Nian-Feng; Pan, Hong-Ying; Li, Ming-Wei; Huang, Hai-Jun

    2015-01-01

    AIM: To assess the efficacy of tenofovir disoproxil fumarate (TDF) in lamivudine (LAM)-resistant patients with a suboptimal response to LAM plus adefovir (ADV). METHODS: We retrospectively analyzed the efficacy of switching to tenofovir disoproxil fumarate in suboptimal responders to lamivudine plus adefovir. Charts were reviewed for LAM-resistant chronic hepatitis B (CHB) patients who visited the Zhejiang Province People’s Hospital and The First Affiliated Hospital, College of Medicine, Zhejiang University, from June 2009 to May 2013. Patients whose serum hepatitis B virus (HBV) DNA remained detectable despite at least 6 mo of LAM plus ADV combination therapy were included. Patients with a suboptimal response to LAM plus ADV were randomized to switch to TDF monotherapy (300 mg/d orally; TDF group) or to continuation with LAM (100 mg/d orally) plus ADV (10 mg/d orally; LAM plus ADV group) and were followed for 48 wk. Serum HBV DNA was determined at baseline and weeks 4, 12, 24, 36, and 48. HBV serological markers and biochemistry were assessed at baseline and weeks 12, 24, and 48. Resistance surveillance and side effects were monitored during therapy. RESULTS: Fifty-nine patient were randomized to switch to TDF (n = 28) or continuation with LAM plus ADV (n = 31). No significant differences were found between the groups at baseline. Prior to TDF therapy, all patients had been exposed to LAM plus ADV for a median of 11 mo (range: 6-24 mo). No difference was seen in baseline serum HBV DNA between the two groups [5.13 ± 1.08 log10 copies/mL (TDF) vs 5.04 ± 31.16 log10 copies/mL (LAM + ADV), P = 0.639]. There was no significant difference in the rates of achieving complete virological response (CVR) at week 4 between the TDF and LAM + ADV groups (17.86% vs 6.45%, P = 0.24). The rate of achieving CVR in the TDF and LAM plus ADV groups was 75% vs 16.13% at week 12, 82.14% vs 22.58% at week 24, 89.29% vs 25.81% at week 36, and 96.43% vs 29.03% at week 48, respectively (P < 0.001). The rate of alanine aminotransferase normalization was significantly higher in the TDF than in the LAM plus ADV group at week 12 (75% vs 17.86%, P < 0.001), but not at week 24 (78.57% vs 54.84%, P = 0.097) or 48 (89.26% vs 67.74%, P = 0.062). Patients were hepatitis B e antigen (HBeAg) positive at baseline. There was no significant difference in HBeAg negativity between the TDF and LAM plus ADV groups at week 48 (4% vs 0%, P = 0.481). There were no drug-related adverse effects at week 48 in either group. CONCLUSION: Switching to TDF monotherapy was superior to continuous add-on therapy in patients with LAM-resistant CHB with a suboptimal response to LAM plus ADV. PMID:25759545

  15. Forage, soil and water quality responses to animal waste application

    E-print Network

    Johnson, Andrew Floyd

    1995-01-01

    applications to bermudagrass (Cynodon dactylon) and ryegrass (Lolium multiflorum Lam) sods were used to quantify N03 leaching below the root zone of a Darco loamy fine sand (loamy, siliceous, thermic Grossarenic Paleudult) located at Overton, Texas. Rates of 0...

  16. School of Chemistry Health and Safety Departmental Fire Stewards (Main Building)

    E-print Network

    Hall, Christopher

    School of Chemistry Health and Safety Departmental Fire Stewards (Main Building) Area Fire Steward Dominic Campopiano 7B Hon Lam Uwe Schneider (Teaching Block) Area Fire Steward Clear Emergency in Area

  17. Faculty Arts & Social Sciences Bachelor of Arts

    E-print Network

    , Jason Major Economics Robert McMath Senior Secondary BC Bachelor of Arts Chan, Kelvin Kwan Lam Major Bachelor of Arts Law, Spencer Shing Lai Major Psychology Richmond Secondary BC Bachelor of Arts Lee, Shara

  18. Lymphangioleiomyomatosis Biomarkers Linked to Lung Metastatic Potential and Cell Stemness

    PubMed Central

    Ruiz de Garibay, Gorka; Herranz, Carmen; Llorente, Alicia; Boni, Jacopo; Serra-Musach, Jordi; Mateo, Francesca; Aguilar, Helena; Gómez-Baldó, Laia; Petit, Anna; Vidal, August; Climent, Fina; Hernández-Losa, Javier; Cordero, Álex; González-Suárez, Eva; Sánchez-Mut, José Vicente; Esteller, Manel; Llatjós, Roger; Varela, Mar; López, José Ignacio; García, Nadia; Extremera, Ana I.; Gumà, Anna; Ortega, Raúl; Plà, María Jesús; Fernández, Adela; Pernas, Sònia; Falo, Catalina; Morilla, Idoia; Campos, Miriam; Gil, Miguel; Román, Antonio; Molina-Molina, María; Ussetti, Piedad; Laporta, Rosalía; Valenzuela, Claudia; Ancochea, Julio; Xaubet, Antoni; Casanova, Álvaro; Pujana, Miguel Angel

    2015-01-01

    Lymphangioleiomyomatosis (LAM) is a rare lung-metastasizing neoplasm caused by the proliferation of smooth muscle-like cells that commonly carry loss-of-function mutations in either the tuberous sclerosis complex 1 or 2 (TSC1 or TSC2) genes. While allosteric inhibition of the mechanistic target of rapamycin (mTOR) has shown substantial clinical benefit, complementary therapies are required to improve response and/or to treat specific patients. However, there is a lack of LAM biomarkers that could potentially be used to monitor the disease and to develop other targeted therapies. We hypothesized that the mediators of cancer metastasis to lung, particularly in breast cancer, also play a relevant role in LAM. Analyses across independent breast cancer datasets revealed associations between low TSC1/2 expression, altered mTOR complex 1 (mTORC1) pathway signaling, and metastasis to lung. Subsequently, immunohistochemical analyses of 23 LAM lesions revealed positivity in all cases for the lung metastasis mediators fascin 1 (FSCN1) and inhibitor of DNA binding 1 (ID1). Moreover, assessment of breast cancer stem or luminal progenitor cell biomarkers showed positivity in most LAM tissue for the aldehyde dehydrogenase 1 (ALDH1), integrin-ß3 (ITGB3/CD61), and/or the sex-determining region Y-box 9 (SOX9) proteins. The immunohistochemical analyses also provided evidence of heterogeneity between and within LAM cases. The analysis of Tsc2-deficient cells revealed relative over-expression of FSCN1 and ID1; however, Tsc2-deficient cells did not show higher sensitivity to ID1-based cancer inhibitors. Collectively, the results of this study reveal novel LAM biomarkers linked to breast cancer metastasis to lung and to cell stemness, which in turn might guide the assessment of additional or complementary therapeutic opportunities for LAM. PMID:26167915

  19. Comparative Study of the Essential Oils of Three Achillea Species from Iran

    Microsoft Academic Search

    K. Jaimand; M. B. Rezaee

    2001-01-01

    The composition of the oils from flowers of three Iranian Achillea species (A. tenuifolia Lam, A. biebersteinii Afan., A. filipendulina Lam.) were collected during the flowering period. The essential oils obtained by steam distillation. The percentage of all three oils were 0.20% v\\/w calculated on the dry weight, analyzed by GC and GC\\/MS, all three oils consisted mainly of monoterpenes.

  20. The mixed problem for the Lame system in a class of Lipschitz domains

    E-print Network

    Brown, Russell M.

    The mixed problem for the Lam´e system in a class of Lipschitz domains Russell M. Brown Department of Mathematics University of Virginia Charlottesville, Virginia im3p@virginia.edu Abstract We consider the mixed problem for the Lam´e system Lu = 0 in u|D = fD on D u = fN on N ( u) Lp() in the class of bounded

  1. The Structure of Bovine Lysosomal ?-Mannosidase Suggests a Novel Mechanism for Low-pH Activation

    Microsoft Academic Search

    Pirkko Heikinheimo; Ronny Helland; Hanna-Kirsti Schrøder Leiros; Ingar Leiros; Solveig Karlsen; Gry Evjen; Raimond Ravelli; Guy Schoehn; Rob Ruigrok; Ole-Kristian Tollersrud; Seán McSweeney; Edward Hough

    2003-01-01

    Lysosomal ?-mannosidase (LAM: EC 3.2.1.24) belongs to the sequence-based glycoside hydrolase family 38 (GH38). Two other mammalian GH38 members, Golgi ?-mannosidase II (GIIAM) and cytosolic ?-mannosidase, are expressed in all tissues. In humans, cattle, cat and guinea pig, lack of lysosomal ?-mannosidase activity causes the autosomal recessive disease ?-mannosidosis. Here, we describe the three-dimensional structure of bovine lysosomal ?-mannosidase (bLAM)

  2. Expression and purification of a functionally active recombinant GDP-mannosyltransferase (PimA) from Mycobacterium tuberculosis H37Rv

    Microsoft Academic Search

    Xiaoling Gu; Mao Chen; Qingzhong Wang; Min Zhang; Baolin Wang; Honghai Wang

    2005-01-01

    Lipoarabinomannans (LAM), especially mannose-capped LAM, abundant in the cell wall of Mycobacterium tuberculosis (Mtb) exhibit a broad spectrum of immunomodulatory functions and emerge as key virulence factors that may be relevant drug targets. The pimA gene of mycobacteria encodes a ?-mannosyltransferase involved in the transfer reaction of the very first mannose from GDP–mannose to the carrier lipid phosphatidyl-myo-inositol, a precursor

  3. Mycobacterium tuberculosis arabinomannan–protein conjugates protect against tuberculosis

    Microsoft Academic Search

    Beston Hamasur; Melles Haile; Andrzej Pawlowski; Ulf Schröder; Ann Williams; Graham Hatch; Graham Hall; Philip Marsh; Gunilla Källenius; Stefan B. Svenson

    2003-01-01

    Lipoarabinomannan (LAM) is a major structural surface component of mycobacteria. Arabinomannan (AM) oligosaccharides derived from LAM of Mycobacterium tuberculosis H37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a 75kDa protein) from M. tuberculosis strain Harlingen. The different AM oligosaccharide (AMOs)–protein conjugate vaccine candidates proved to be highly immunogenic,

  4. Coronin-1A Links Cytoskeleton Dynamics to TCRab-Induced Cell Signaling

    E-print Network

    Boyer, Edmond

    ,2,3. , Be´atrice Nal1,2,3,6. , Christophe Verthuy1,2,3. , Claude Boyer1,2,3 , David Lam1,2,3 , Lionel Chasson1,2,3 , Vincent Nieoullon4 , Genevie`ve Chazal4 , Xiao-Jun Guo1,2,3,5 , Hai-Tao He1,2,3 , Dominique of other lympho/hematopoietic lineages. Citation: Mugnier B, Nal B, Verthuy C, Boyer C, Lam D, et al. (2008

  5. Insights into the population structure of Mycobacterium tuberculosis using spoligotyping and RDRio in a southeastern Brazilian prison unit.

    PubMed

    Huber, Fé Dagmar; Sánchez, Alexandra; Gomes, Harrison Magdinier; Vasconcellos, Sidra; Massari, Véronique; Barreto, Angela; Cesconi, Vanderci; de Almeida Machado, Silvia Maria; Gomgnimbou, Michel K; Sola, Christophe; Larouzé, Bernard; Suffys, Philip Noel; Saad, Maria Helena Féres

    2014-08-01

    Tuberculosis (TB) is still a serious public health problem, continuing to be an important threat for confined populations. We used spoligotyping to estimate the genotypic clades of Mycobacterium tuberculosis isolates from inmates in two blocks in a southeastern Brazilian prison unit, with TB incidence rate of 8185/100.000. The Latin American Mediterranean (LAM) clade is well represented in the country, and the LAM specific molecular markers, RD(Rio) large sequence polymorphism and the SNP on the Rv3062 [ligB(1212)], were used to characterize spoligotype signatures from prison isolates. Typing of RD(Rio) and ligB increase LAM clade from 66.7% (n=72/108) to 69.4% (n=75). The LAM2 SIT17 (n=23) and SIT179 (n=12) signatures comprised one third of all isolates, followed by Haarlem (11.5%, n=12), T (8.7%, n=9) and X (5.7%, n=6) clades. Strains with unknown signatures represented 5.5% (n=6), and four (3.7%) did not match any lineage. We observed RD(Rio) among 64 (59.2%) isolates, and 54 (50%) were of the LAM clade. In particular, the LAM2/RD(Rio) sub-lineage was significantly associated with clustering (p=0.02) and its frequency was higher (32%) when compared to that of the previous general TB cases in RJ (4.29%). Overall cluster frequency defined by spoligotyping/IS6110-RFLP was 62%. The two evolutionary markers helped to evaluate some LAM signature misconceptions and demonstrate that LAM2/RD(Rio) was found with high frequency, hitherto being unnoticed. All these data, allied to high clustering, imply that public health measures to minimize the escalation of TB in prison is essential, and both spoligotyping as well as RD(Rio) would be useful tools to monitor the effects of the measures with respect to M. tuberculosis lineage variation. PMID:24907670

  6. Finite-gap difference opeators with elliptic coefficients and their spectral curves

    Microsoft Academic Search

    A. Zabrodin

    1999-01-01

    We review recent results on the finite-gap properties of difference operators with elliptic coefficients and give explicit characterization of spectral curves for difference analogues of the higher Lam\\\\'e operators. This curve parametrizes double-Bloch solutions to the difference Lam\\\\'e equation. The curve depends on a positive integer number \\\\ell, related to its genus g by g=2\\\\ell, and two continuous parameters: the

  7. Combination Therapy of Murine Mucormycosis or Aspergillosis with Iron Chelation, Polyenes, and Echinocandins?

    PubMed Central

    Ibrahim, Ashraf S.; Gebremariam, Teclegiorgis; Luo, Guanpingsheng; Fu, Yue; French, Samuel W.; Edwards, John E.; Spellberg, Brad

    2011-01-01

    Liposomal amphotericin B (LAmB) combined wither either micafungin or deferasirox was synergistic in previous murine studies with mucormycosis or aspergillosis. We hypothesized that triple therapy using LAmB, micafungin, and deferasirox could further improve outcomes of mucormycosis or aspergillosis. Triple therapy improved survival and reduced tissue fungal burden of mice with mucormycosis and to a lesser extent with aspergillosis. Continued investigation into the use of triple therapy against mucormycosis and aspergillosis is warranted. PMID:21263057

  8. A review on research and development of laser assisted turning

    Microsoft Academic Search

    Kwang-Sun Kim; Jae-Hyun Kim; Jun-Young Choi; Choon-Man Lee

    2011-01-01

    LAM(Laser assisted machining) is machining method that performs a machining for workpieces using a laser preheating process.\\u000a By combining the LAM and CNC lathe using laser beam, it could be named LAT (Laser Assisted Turning). A machining process in\\u000a LAT has been applied at 1200?1400°C using a specific cutting tool, after preheating a workpiece using laser beam as about\\u000a 2000°C.

  9. High-throughput monitoring of integration site clonality in preclinical and clinical gene therapy studies

    PubMed Central

    Giordano, Frank A; Appelt, Jens-Uwe; Link, Barbara; Gerdes, Sebastian; Lehrer, Christina; Scholz, Simone; Paruzynski, Anna; Roeder, Ingo; Wenz, Frederik; Glimm, Hanno; von Kalle, Christof; Grez, Manuel; Schmidt, Manfred; Laufs, Stephanie

    2015-01-01

    Gene transfer to hematopoietic stem cells with integrating vectors not only allows sustained correction of monogenic diseases but also tracking of individual clones in vivo. Quantitative real-time PCR (qPCR) has been shown to be an accurate method to quantify individual stem cell clones, yet due to frequently limited amounts of target material (especially in clinical studies), it is not useful for large-scale analyses. To explore whether vector integration site (IS) recovery techniques may be suitable to describe clonal contributions if combined with next-generation sequencing techniques, we designed artificial ISs of different sizes which were mixed to simulate defined clonal situations in clinical settings. We subjected all mixes to either linear amplification–mediated PCR (LAM-PCR) or nonrestrictive LAM-PCR (nrLAM-PCR), both combined with 454 sequencing. We showed that nrLAM-PCR/454-detected clonality allows estimating qPCR-detected clonality in vitro. We then followed the kinetics of two clones detected in a patient enrolled in a clinical gene therapy trial using both, nrLAM-PCR/454 and qPCR and also saw nrLAM-PCR/454 to correlate to qPCR-measured clonal contributions. The method presented here displays a feasible high-throughput strategy to monitor clonality in clinical gene therapy trials is at hand. PMID:26052530

  10. On Some Genetic Aspects of Phage ? Resistance in E. COLI K12

    PubMed Central

    Thirion, J. P.; Hofnung, M.

    1972-01-01

    Most mutations rendering E. coli K12 resistant to phage ?, map in two genetic regions malA and malB.—The malB region contains a gene lamB specifically involved in the ? receptor synthesis. Twenty-one independent lamB mutations studied by complementation belonged to a single cistron. This makes it very likely that lamB is monocistronic. Among the lamB mutants some are still sensitive to a host range mutant of phage ?. Mutations mapping in a proximal gene essential for maltose metabolism inactivate gene lamB by polarity confirming that both genes are part of the same operon. Because cases of intracistronic complementation have been found, the active lamB product may be an oligomeric protein.—Previously all ? resistant mutations in the malA region have been shown to map in the malT cistron. malT is believed to be a positive regulatory gene necessary for the induction of the "maltose operons" in the malA region and in the malB region of the E. coli K12 genetic map. No trans dominant malT mutation have been found. Therefore if they exist, they occur at a frequency of less than 10-8, or strongly reduce the growth rate of the mutants. PMID:4558324

  11. Induction of early-response genes KC and JE by mycobacterial lipoarabinomannans: regulation of KC expression in murine macrophages by Lsh/Ity/Bcg (candidate Nramp).

    PubMed Central

    Roach, T I; Chatterjee, D; Blackwell, J M

    1994-01-01

    The murine chromosome 1 gene Lsh/Ity/Bcg (candidate Nramp) regulates macrophage activation for antimicrobial activity against Salmonella typhimurium, Leishmania donovani, and Mycobacterium spp. To determine early events in the activation pathway, the ability of mycobacterial lipoarabinomannan (LAM) to induce early gene (KC and JE) expression in macrophages from susceptible (S) C57BL/10ScSn (Lshs) and congenic resistant (R) B10.L-Lshr mice was investigated. Stimulation with 1.8 microgram of arabinofuranosyl-terminated LAM (AraLAM) per ml resulted in similar kinetics for KC or JE expression in S and R macrophages. However, whereas JE/glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA ratios remained equivalent, R macrophages consistently showed enhanced KC/GAPDH ratios within 30 to 40 min of stimulation compared with S macrophages. Significant differences in KC/GAPDH ratios were observed throughout the peak period (0.5 to 6 h) of the KC response and with doses of AraLAM ranging from 0.01 to 2.5 micrograms/ml. Heavily mannosylated LAM from virulent Mycobacterium tuberculosis Erdman, in doses of up to 2.5 micrograms/ml, failed to stimulate KC or JE in S or R macrophages. Gamma interferon alone (25 U/ml) stimulated equivalent JE expression in S and R macrophages and synergized with AraLAM to enhance JE in both. In contrast, AraLAM-induced KC expression was inhibited in the presence of gamma interferon. Agonist/inhibitor studies were undertaken to determine the signal transduction pathways mediating KC expression. The protein kinase C (PKC) inhibitor Calphostin C (200 nM) inhibited AraLAM-induced KC by 34% +/- 4% in S macrophages and 43% +/- 5% in R macrophages; the cyclic AMP-dependent PKA inhibitor KT5720 (2 microM) inhibited AraLAM-induced KC by 33% +/- 4% (S) and 25% +/- 5% (R). A role for Ca2+ was indicated because ionophore alone stimulated KC expression and synergized with AraLAM to give a dramatically enhanced response. Induction of KC was also inhibited by (i) blocking constitutive nitric oxide (NO) production by preincubation of macrophages with NG-monomethyl-L-arginine (400 microM) (48% +/- 8% [S] and 40% +/- 11% [R]) and (ii) incubation of macrophages with the cyclic GMP-dependent kinase inhibitor KT5823 (4 microM) (65% +/- 4% [S] and 72% +/- 6% [R]). The manner in which these PKC-, PKA-, and Ca(2+)-dependent, NO-mediated cyclic GMP-dependent kinase signal transduction pathways may relate to function of the candidate Lsh/Ity/Bcg gene Nramp is discussed. Images PMID:8132324

  12. Induction of early-response genes KC and JE by mycobacterial lipoarabinomannans: regulation of KC expression in murine macrophages by Lsh/Ity/Bcg (candidate Nramp).

    PubMed

    Roach, T I; Chatterjee, D; Blackwell, J M

    1994-04-01

    The murine chromosome 1 gene Lsh/Ity/Bcg (candidate Nramp) regulates macrophage activation for antimicrobial activity against Salmonella typhimurium, Leishmania donovani, and Mycobacterium spp. To determine early events in the activation pathway, the ability of mycobacterial lipoarabinomannan (LAM) to induce early gene (KC and JE) expression in macrophages from susceptible (S) C57BL/10ScSn (Lshs) and congenic resistant (R) B10.L-Lshr mice was investigated. Stimulation with 1.8 microgram of arabinofuranosyl-terminated LAM (AraLAM) per ml resulted in similar kinetics for KC or JE expression in S and R macrophages. However, whereas JE/glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA ratios remained equivalent, R macrophages consistently showed enhanced KC/GAPDH ratios within 30 to 40 min of stimulation compared with S macrophages. Significant differences in KC/GAPDH ratios were observed throughout the peak period (0.5 to 6 h) of the KC response and with doses of AraLAM ranging from 0.01 to 2.5 micrograms/ml. Heavily mannosylated LAM from virulent Mycobacterium tuberculosis Erdman, in doses of up to 2.5 micrograms/ml, failed to stimulate KC or JE in S or R macrophages. Gamma interferon alone (25 U/ml) stimulated equivalent JE expression in S and R macrophages and synergized with AraLAM to enhance JE in both. In contrast, AraLAM-induced KC expression was inhibited in the presence of gamma interferon. Agonist/inhibitor studies were undertaken to determine the signal transduction pathways mediating KC expression. The protein kinase C (PKC) inhibitor Calphostin C (200 nM) inhibited AraLAM-induced KC by 34% +/- 4% in S macrophages and 43% +/- 5% in R macrophages; the cyclic AMP-dependent PKA inhibitor KT5720 (2 microM) inhibited AraLAM-induced KC by 33% +/- 4% (S) and 25% +/- 5% (R). A role for Ca2+ was indicated because ionophore alone stimulated KC expression and synergized with AraLAM to give a dramatically enhanced response. Induction of KC was also inhibited by (i) blocking constitutive nitric oxide (NO) production by preincubation of macrophages with NG-monomethyl-L-arginine (400 microM) (48% +/- 8% [S] and 40% +/- 11% [R]) and (ii) incubation of macrophages with the cyclic GMP-dependent kinase inhibitor KT5823 (4 microM) (65% +/- 4% [S] and 72% +/- 6% [R]). The manner in which these PKC-, PKA-, and Ca(2+)-dependent, NO-mediated cyclic GMP-dependent kinase signal transduction pathways may relate to function of the candidate Lsh/Ity/Bcg gene Nramp is discussed. PMID:8132324

  13. Clostridium huakuii sp. nov., an anaerobic, acetogenic bacterium isolated from methanogenic consortia.

    PubMed

    Ruan, Zhiyong; Wang, Yanwei; Zhang, Chi; Song, Jinlong; Zhai, Yi; Zhuang, Yan; Wang, Huimin; Chen, Xiaorong; Li, Yanting; Zhao, Bingqiang; Zhao, Bin

    2014-12-01

    A Gram-staining-positive, spore-forming, obligately anaerobic, acetogenic bacterium, designated LAM1030(T), was isolated from methanogenic consortia enriched from biogas slurry collected from the large-scale anaerobic digester of Modern Farming Corporation in Hebei Province, China. Cells of strain LAM1030(T) were motile, straight or spiral-rod-shaped. Strain LAM1030(T) could utilize glucose, fructose, maltose, galactose, lactose, sucrose, cellobiose, mannitol, pyruvate, succinic acid and tryptophan as the sole carbon source. Acetic acid, isovaleric acid and butanoic acid were the main products of glucose fermentation. Sodium sulfite was used as an electron acceptor. Growth of strain LAM1030(T) was completely inhibited by the addition of ampicillin, tetracycline, gentamicin or erythromycin at a concentration of 20 µg ml(-1). The main polar lipids of strain LAM1030(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, 11 unknown glycolipids and two unknown phospholipids. No respiratory quinone was detected. The major fatty acids of strain LAM1030(T) were C16?:?0 (21.1?%), C14?:?0 (10.3?%), summed feature 9 (including C16:0 10-methyl and/or iso-C17:1 ?9c) (11.3% ), summed feature 3 (including C16:1 ?7c and/or C16:1 ?6c) (10.6% ) and iso-C15?:?0 (6.6?%). Analysis of the 16S rRNA gene sequence indicated that strain LAM1030(T) belonged to the genus Clostridium and was most closely related to Clostridium subterminale DSM 6970(T), Clostridium thiosulfatireducens DSM 13105(T) and Clostridium sulfidigenes DSM 18982(T), with 97.0, 96.9 and 96.8?% similarity, respectively. The G+C content of the genomic DNA of strain LAM1030(T) was 31.2±0.3 mol%. On the basis of its phenotypic, phylogenetic and chemotaxonomic characterization, strain LAM1030(T) is suggested to represent a novel species of the genus Clostridium, for which the name Clostridium huakuii sp. nov. is proposed. The type strain is LAM1030(T) (?=?ACCC 00698(T)?=?JCM 19186(T)). PMID:25230767

  14. Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients

    PubMed Central

    2012-01-01

    Background Lamivudine (LAM) is associated with the highest known rate of resistance mutations among nucleotide analogs used to treat chronic hepatitis B virus (HBV) infection. Despite this, LAM continues in widespread use, especially in combination therapies. The primary LAM resistance mutation (rtM204V/I) occurs in the YMDD motif of HBV polymerase. The aim of this study was to characterize Brazilian HBV isolates from acute and chronic cases by direct sequencing, and to identify HBV quasispecies in the YMDD motif using a pyrosequencing method capable of detecting single-nucleotide polymorphisms. HBV DNA from serum samples of 20 individuals with acute HBV infection and 44 with chronic infection undergoing antiviral therapies containing LAM were analyzed by direct sequencing and pyrosequencing methods. Results Phylogenic analyses of direct-sequenced isolates showed the expected genotypes (A, D and F) for the Brazilian population in both acute and chronic infections. However, within genotype A isolates, subgenotype A2 was more frequently detected in acute cases than in chronic cases (P?=?0.012). As expected, none of the individuals with acute hepatitis B had LAM-resistant isolates as a dominant virus population, whether detected by direct sequencing or pyrosequencing. However, pyrosequencing analyses showed that 45% of isolates (9/20) had minor subpopulations (4-17%) of LAM-resistant isolates. Among chronic patients undergoing LAM treatment, YMDD mutants were frequently found as a dominant virus population. In cases where wild-type virus was the dominant population, subpopulations of YMDD variants were usually found, demonstrating the complexity of HBV quasispecies. Conclusions YMDD variants were frequently detected as a minor population in acute HBV infection. The occurrence of pre-existing variants may lead to a high frequency of resistant mutants during antiviral therapy in the chronic phase. In chronic infection, detection of YMDD variants before virological or biochemical breakthrough might contribute to making better therapy choices and thus improving treatment outcome. PMID:22672436

  15. Management of lymphangioleiomyomatosis

    PubMed Central

    Moss, Joel

    2014-01-01

    Lymphangioleiomyomatosis (LAM), a multisystem disease affecting almost exclusively women, is characterized by cystic lung destruction and presents with dyspnea, recurrent pneumothoraxes, chylous effusions, lymphangioleiomyomas, and angiomyolipomas. It is caused by the proliferation of a cancer-like LAM cell that possesses a mutation in either the tuberous sclerosis complex (TSC)1 or TSC2 genes. This article reviews current therapies and new potential treatments that are currently undergoing investigation. The major development in the treatment of LAM is the discovery of two mammalian target of rapamycin (mTOR) inhibitors, sirolimus and everolimus, as effective drugs. However, inhibition of mTOR increases autophagy, which may lead to enhanced LAM cell survival. Use of autophagy inhibitors, for example, hydroxychloroquine, in combination with sirolimus is now the subject of an ongoing drug trial (SAIL trial). Another consequence of mTOR inhibition by sirolimus is an increase in Rho activity, resulting in reduced programmed cell death. From these data, the concept evolved that a combination of sirolimus with disruption of Rho activity with statins (e.g. simvastatin) may increase TSC-null cell death and reduce LAM cell survival. A combined trial of sirolimus with simvastatin is under investigation (SOS trial). Since LAM occurs primarily in women and TSC-null cell survival and tumor growth is promoted by estrogens, the inhibition of aromatase to block estrogen synthesis is currently undergoing study (TRAIL trial). Other targets, for example, estrogen receptors, mitogen-activated protein kinase inhibitors, vascular endothelial growth factor-D signaling pathway, and Src kinase, are also being studied in experimental model systems. As in the case of cancer, combination therapy may become the treatment of choice for LAM. PMID:25580270

  16. Multidrug-Resistant Tuberculosis in Panama Is Driven by Clonal Expansion of a Multidrug-Resistant Mycobacterium tuberculosis Strain Related to the KZN Extensively Drug-Resistant M. tuberculosis Strain from South Africa

    PubMed Central

    Lanzas, Fedora; Karakousis, Petros C.; Sacchettini, James C.

    2013-01-01

    Multidrug-resistant tuberculosis (MDR-TB) is a significant health problem in Panama. The extent to which such cases are the result of primary or acquired resistance and the strain families involved are unknown. We performed whole-genome sequencing of a collection of 66 clinical MDR isolates, along with 31 drug-susceptible isolates, that were isolated in Panama between 2001 and 2010; 78% of the MDR isolates belong to the Latin American-Mediterranean (LAM) family. Drug resistance mutations correlated well with drug susceptibility profiles. To determine the relationships among these strains and to better understand the acquisition of resistance mutations, a phylogenetic tree was constructed based on a genome-wide single-nucleotide polymorphism analysis. The phylogenetic tree shows that the isolates are highly clustered, with a single strain (LAM9-c1) accounting for nearly one-half of the MDR isolates (29/66 isolates). The LAM9-c1 strain was most prevalent among male patients of working age and was associated with high mortality rates. Members of this cluster all share identical mutations conferring resistance to isoniazid (KatG S315T mutation), rifampin (RpoB S531L mutation), and streptomycin (rrs C517T mutation). This evidence of primary resistance supports a model in which MDR-TB in Panama is driven by clonal expansion and ongoing transmission of several strains in the LAM family, including the highly successful MDR strain LAM9-c1. The phylogenetic analysis also shows that the LAM9-c1 strain is closely related to the KwaZulu-Natal (KZN) extensively drug-resistant TB strain identified in KwaZulu-Natal, South Africa. The LAM9-c1 and KZN strains likely arose from a recent common ancestor that was transmitted between Panama and South Africa and had the capacity to tolerate an accumulation of multiple resistance mutations. PMID:23884993

  17. Liposomal amphotericin B: what is its role in 2008?

    PubMed

    Lanternier, F; Lortholary, O

    2008-05-01

    Although our antifungal armamentarium has been enlarged recently with new azoles (voriconazole and posaconazole) and echinocandins (caspofungin, micafungin, anidulafungin), the polyenes still have an important role in antifungal strategies because of their extended antifungal spectrum and rarity of mycological resistance. The use of conventional amphotericin B deoxycholate is limited by substantial toxicity that is either infusion-related or associated with renal failure. Its lipid derivatives, particularly liposomal amphotericin B (LAmB), are less nephrotoxic while maintaining a broad antifungal spectrum. LAmB is active against most Candida spp., including Candida glabrata and Candida parapsilosis, and against more resistant, emerging yeasts species such as Rhodotorula spp., Geotrichum spp. and Trichosporon spp.. LAmB is also active against Cryptococcus spp. and all dimorphic fungi such as Histoplasma, Blastomyces, Coccidioidomyces, and Paracoccidiodomyces. The antifungal spectrum of LAmB is particularly interesting with regard to filamentous fungi, with marked activity against Aspergillus spp. and agents of zygomycosis. The latter might emerge during long-term treatment with voriconazole or an echinocandin, as these organisms are resistant to these drugs. We review here the role of LAmB in the current antifungal management strategy, which is based on results obtained in prospective trials. LAmB can be retained as first-line treatment for human immunodeficiency virus (HIV)-positive patients with disseminated histoplasmosis and cryptococcosis, even in the setting of renal impairment or concomitant administration of potentially nephrotoxic drugs. In addition, there is sufficient evidence that the drug should be a major consideration for the empirical treatment of persistent febrile neutropenia or as an alternative to for patients with invasive aspergillosis, for those at risk of renal impairment, major drug-drug interaction or liver insufficiency, particularly in the situation of an established azole intolerance. The primary licensed indication for LAmB is empirical treatment. When zygomycosis is suspected or has been documented, high doses of LAmB should be prescribed. Finally, LAmB may also be considered as a therapeutic option for the management of candidaemia and remains a cornerstone for the treatment of some visceral localisations during systemic candidosis. PMID:18430132

  18. Diagnostic accuracy of a point-of-care urine test for tuberculosis screening among newly-diagnosed hiv-infected adults: a prospective, clinic-based study

    PubMed Central

    2014-01-01

    Background A rapid diagnostic test for active tuberculosis (TB) at the clinical point-of-care could expedite case detection and accelerate TB treatment initiation. We assessed the diagnostic accuracy of a rapid urine lipoarabinomannan (LAM) test for TB screening among HIV-infected adults in a TB-endemic setting. Methods We prospectively enrolled newly-diagnosed HIV-infected adults (?18 years) at 4 outpatient clinics in Durban from Oct 2011-May 2012, excluding those on TB therapy. A physician evaluated all participants and offered CD4 cell count testing. Trained study nurses collected a sputum sample for acid-fast bacilli smear microscopy (AFB) and mycobacterial culture, and performed urine LAM testing using Determine™ TB LAM in the clinic. The presence of a band regardless of intensity on the urine LAM test was considered positive. We defined as the gold standard for active pulmonary TB a positive sputum culture for Mycobacterium tuberculosis. Diagnostic accuracy of urine LAM was assessed, alone and in combination with smear microscopy, and stratified by CD4 cell count. Results Among 342 newly-diagnosed HIV-infected participants, 190 (56%) were male, mean age was 35.6 years, and median CD4 was 182/mm3. Sixty participants had culture-positive pulmonary TB, resulting in an estimated prevalence of 17.5% (95% CI 13.7-22.0%). Forty-five (13.2%) participants were urine LAM positive. Mean time from urine specimen collection to LAM test result was 40 minutes (95% CI 34–46 minutes). Urine LAM test sensitivity was 28.3% (95% CI 17.5-41.4) overall, and 37.5% (95% CI 21.1-56.3) for those with CD4 count <100/mm3, while specificity was 90.1% (95% CI 86.0-93.3) overall, and 86.9% (95% CI 75.8-94.2) for those with CD4?LAM test had low sensitivity for TB screening among newly-diagnosed HIV-infected adults, but improved sensitivity when combined with sputum smear microscopy. PMID:24571362

  19. Generation of a human hepatoma cell line supporting efficient replication of a lamivudine resistant hepatitis B virus.

    PubMed

    Zhang, Yijun; Zhang, Yongmei; Kang, Yaoyue; Wang, Jinyu; Liu, Hongyan; Zhu, Haoxiang; Qin, Yanli; Mao, Richeng; Lin, Xu; Lu, Mengji; Zhang, Jiming

    2014-06-01

    Emergence of lamivudine (LAM) resistance causes treatment failure in patients with chronic hepatitis B and compromise the efficacy of subsequent salvage therapies with other nucleot(s)ide analogs (NAs). Establishment of cell-based assays supporting LAM-resistant hepatitis B virus (HBV) replication will not only provide tools for investigating the replication property, but also screening for antiviral agents efficiently inhibiting the replication of LAM-resistant HBV variants. Accordingly, a human hepatoma (HepG2)-derived cell line was established by stable transfection of a plasmid containing a 1.2 unit length of HBV genome harboring rtL180M and rtM204V mutations that confer LAM resistance. In addition to support efficient viral genome replication, the cell line also produces high levels of HBV virions and subviral particles. As expected, HBV DNA replication in this cell line is completely resistant to lamivudine, but sensitive to adefovir (ADV), entecavir (ETV) and tenofovir (TDF). The cell line is suitable for screening for antiviral agents that inhibit LAM-resistant HBV replication and inhibitors of HBsAg biosynthesis and secretion, which may reduce HBsAg antigenemia and ultimately help to restore host antiviral immune response against HBV and cure chronic HBV infection. PMID:24583110

  20. The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru

    PubMed Central

    Grandjean, Louis; Iwamoto, Tomotada; Lithgow, Anna; Gilman, Robert H; Arikawa, Kentaro; Nakanishi, Noriko; Martin, Laura; Castillo, Edith; Alarcon, Valentina; Coronel, Jorge; Solano, Walter; Aminian, Minoo; Guezala, Claudia; Rastogi, Nalin; Couvin, David; Sheen, Patricia; Zimic, Mirko; Moore, David AJ

    2015-01-01

    Background The comparison of Mycobacterium tuberculosis bacterial genotypes with phenotypic, demographic, geospatial and clinical data improves our understanding of how strain lineage influences the development of drug-resistance and the spread of tuberculosis. Methods To investigate the association of Mycobacterium tuberculosis bacterial genotype with drug-resistance. Drug susceptibility testing together with genotyping using both 15-loci MIRU-typing and spoligotyping, was performed on 2,139 culture positive isolates, each from a different patient in Lima, Peru. Demographic, geospatial and socio-economic data were collected using questionnaires, global positioning equipment and the latest national census. Results The Latin American Mediterranean (LAM) clade (OR 2.4, p<0.001) was significantly associated with drug-resistance and alone accounted for more than half of all drug resistance in the region. Previously treated patients, prisoners and genetically clustered cases were also significantly associated with drug-resistance (OR's 2.5, 2.4 and 1.8, p<0.001, p<0.05, p<0.001 respectively). Conclusions Tuberculosis disease caused by the LAM clade was more likely to be drug resistant independent of important clinical, genetic and socio-economic confounding factors. Explanations for this include; the preferential co-evolution of LAM strains in a Latin American population, a LAM strain bacterial genetic background that favors drug-resistance or the "founder effect" from pre-existing LAM strains disproportionately exposed to drugs. PMID:25984723

  1. In Vitro and In Vivo Evaluations of the Activities of Lauric Acid Monoester Formulations against Staphylococcus aureus

    PubMed Central

    Rouse, Mark S.; Rotger, Margalida; Piper, Kerryl E.; Steckelberg, James M.; Scholz, Matthew; Andrews, Jeffrey; Patel, Robin

    2005-01-01

    Due to increasing mupirocin resistance, alternatives for Staphylococcus aureus nasal decolonization are needed. Lauric acid monoesters combined with lactic, mandelic, malic, or benzoic acid are being evaluated as possible alternatives. We determined the in vitro activity of 13 lauric acid monoester (LAM) formulations and mupirocin against 30 methicillin-susceptible S. aureus (MSSA) isolates and 30 methicillin-resistant S. aureus (MRSA) isolates. We then used a murine model of MRSA nasopharyngeal colonization to compare the in vivo activity of mupirocin with three LAM formulations. MSSA and MRSA MIC90 values were 0.25 ?g/ml for mupirocin and ?4 ?l/ml for all LAM formulations tested. Hsd:ICR mice were challenged with 108 CFU/naris MRSA. Five days later, S. aureus colonization was documented by culture. Treatment with bland, mupirocin, or one of three LAM ointments was then administered unblinded thrice daily for 2 days. Three days after treatment, both anterior nares were cultured for S. aureus. Administration of 128774-49E or 128774-53A was associated with greater eradication of MRSA carriage (24/34 [71%] or 33/40 [83%]) of animals, respectively) than bland ointment (12/38 [32%]) (P < 0.005). 128774-53A administration resulted in greater MRSA carriage eradication than mupirocin (19/38 [50%]) (P < 0.005) in this model. LAM formulations warrant evaluation for S. aureus nasal decolonization in humans. PMID:16048923

  2. A Low Viral Load Predicts a Higher Initial Virologic Response to Adefovir in Patients with Lamivudine-Resistant Chronic Hepatitis B

    PubMed Central

    Shin, Su Rin; Gwak, Geum-Youn; Choi, Moon Seok; Lee, Joon Hyoek; Paik, Seung Woon; Yoo, Byung Chul

    2010-01-01

    Background/Aims Adefovir (ADV) is the preferred drug for treating lamivudine (LAM)-resistant hepatitis B. However, not all patients who face virologic breakthrough during LAM treatment respond to ADV. The aim of this study was to determine the factors associated with efficacy of ADV in LAM-resistant hepatitis B patients. Methods The medical records of 231 patients who received ADV due to LAM-resistance were reviewed. Efficacy was assessed by the initial virologic response (IVR), defined as hepatitis B virus (HBV) DNA not being undetectable by real-time PCR at 6 months of ADV treatment. Results Seventy patients (30%) achieved IVR. While 'add-on' modality, hepatitis B e antigen (HBeAg) negativity, and low baseline HBV DNA levels were associated with IVR in univariate analysis, multivariate analysis revealed HBeAg status and the DNA level to be the significant factors. The probability of IVR achievement increased sharply per each log10 copies/mL decrement in the baseline viral load, which was 133 times in patients who had HBV DNA <105 copies/mL compared with those who had ?108 copies/mL. Conclusions Factors associated with the IVR were HBeAg negativity and a low baseline viral load. Therefore, when virologic breakthrough with genotypic resistance emerges during LAM therapy, ADV treatment should be considered immediately before further increases in viral load. Additional long-term follow-up data are warranted. PMID:21253304

  3. Towards personalised therapy for lymphangioleiomyomatosis: lessons from cancer.

    PubMed

    El-Chemaly, Souheil; Henske, Elizabeth P

    2014-03-01

    Lymphangioleiomyomatosis (LAM) is a rare cystic, destructive lung disease occurring almost exclusively in females. Bi-allelic inactivating tuberous sclerosis complex (TSC) gene mutations occur in LAM cells, resulting in activation of the mTORC1 pathway. Pivotal clinical trials have demonstrated that inhibition of mTORC1 with sirolimus can induce a partial response of TSC-associated tumours and decrease the rate of lung function decline in females with LAM. Many parallels have been identified between LAM pathogenesis and neoplasia. Here, we highlight three key nodes through which advances in cancer therapy can streamline future innovation in clinical LAM research with parallels to breast and prostate cancer. These include: 1) hormonally targeted therapies to achieve true disease-free complete remissions; 2) the use of vascular endothelial growth factor-D and other plasma biomarkers to streamline early-phase clinical trials; and 3) the utilisation of histological and molecular features of biopsy material to enable patient stratification and personalised therapies. PMID:24591659

  4. Efficacy of Lamivudine or Entecavir against Virological Rebound after Achieving HBV DNA Negativity in Chronic Hepatitis B Patients

    PubMed Central

    Miyauchi, Tomoo; Kanda, Tatsuo; Shinozaki, Masami; Kamezaki, Hidehiro; Wu, Shuang; Nakamoto, Shingo; Kato, Kazuki; Arai, Makoto; Mikami, Shigeru; Sugiura, Nobuyuki; Kimura, Michio; Goto, Nobuaki; Imazeki, Fumio; Yokosuka, Osamu

    2013-01-01

    Nucleos(t)ide analogues (NAs) lead to viral suppression and undetectable hepatitis B virus (HBV) DNA in some individuals infected with HBV, but the rate of virological rebound has been unknown in such patients. We examined the prevalence of virological rebound of HBV DNA among NA-treated patients with undetectable HBV DNA. We retrospectively analyzed 303 consecutive patients [158 entecavir (ETV)- and 145 lamivudine (LAM)-treated] who achieved HBV DNA negativity, defined as HBV DNA < 3.7 log IU/mL for at least 3 months. They were followed up and their features, including their rates of viral breakthrough, were determined. Viral rebound after HBV DNA negativity was not observed in the ETV-group. Viral rebound after HBV DNA negativity occurred in 38.7% of 62 HBe antigen-positive patients in the LAM-group. On multivariate analysis, age was an independent factor for viral breakthrough among these patients (P = 0.035). Viral rebound after HBV DNA negativity occurred in 29.1% of 79 HBe antigen-negative patients in the LAM-group. Differently from LAM, ETV could inhibit HBV replication once HBV DNA negativity was achieved. In contrast, LAM could not inhibit HBV replication even if HBV negativity was achieved in the early phase. Attention should be paid to these features in clinical practice. PMID:23569428

  5. Mycobacterium tuberculosis Lipoprotein LprG Binds Lipoarabinomannan and Determines Its Cell Envelope Localization to Control Phagolysosomal Fusion

    PubMed Central

    Athman, Jaffre J.; Shi, Libin; Wearsch, Pamela A.; McDonald, David; Banaei, Niaz; Boom, W. Henry; Jackson, Mary; Harding, Clifford V.

    2014-01-01

    Mycobacterium tuberculosis (Mtb) virulence is decreased by genetic deletion of the lipoprotein LprG, but the function of LprG remains unclear. We report that LprG expressed in Mtb binds to lipoglycans, such as lipoarabinomannan (LAM), that mediate Mtb immune evasion. Lipoglycan binding to LprG was dependent on both insertion of lipoglycan acyl chains into a hydrophobic pocket on LprG and a novel contribution of lipoglycan polysaccharide components outside of this pocket. An lprG null mutant (Mtb ?lprG) had lower levels of surface-exposed LAM, revealing a novel role for LprG in determining the distribution of components in the Mtb cell envelope. Furthermore, this mutant failed to inhibit phagosome-lysosome fusion, an immune evasion strategy mediated by LAM. We propose that LprG binding to LAM facilitates its transfer from the plasma membrane into the cell envelope, increasing surface-exposed LAM, enhancing cell envelope integrity, allowing inhibition of phagosome-lysosome fusion and enhancing Mtb survival in macrophages. PMID:25356793

  6. The iron chelator deferasirox enhances liposomal amphotericin B efficacy in treating murine invasive pulmonary aspergillosis

    PubMed Central

    Ibrahim, Ashraf S.; Gebremariam, Teclegiorgis; French, Samuel W.; Edwards, John E.; Spellberg, Brad

    2010-01-01

    Objectives Increased bone marrow iron levels in patients with haematological malignancies is an independent risk factor for developing invasive pulmonary aspergillosis (IPA), suggesting an important role for iron uptake in the pathogenesis of IPA. We sought to determine the potential for combination therapy with the iron chelator deferasirox?+?liposomal amphotericin B (LAmB) to improve the outcome of murine IPA compared with LAmB monotherapy. Methods In vitro MIC and minimum fungicidal concentration (MFC) values of the iron chelator, deferasirox, for Aspergillus fumigatus were determined by microdilution assay. In addition, we studied the efficacy of deferasirox alone or combined with LAmB in treating immunocompromised mice infected with A. fumigatus via inhalation. Results Deferasirox was cidal in vitro against A. fumigatus, with an MIC and MFC of 25 and 50 mg/L, respectively. Deferasirox monotherapy modestly prolonged survival of mice with IPA. Combination deferasirox?+?LAmB therapy synergistically improved survival and reduced lung fungal burden compared with either monotherapy alone. Conclusions Iron chelation therapy with deferasirox alone or in combination with LAmB is effective in treating experimental IPA. Further study of deferasirox is warranted as adjunctive therapy for IPA infections. PMID:19942619

  7. Lamivudine resistance leading to de novo hepatitis B infection in recipients of hepatitis B core antibody positive liver allografts.

    PubMed

    Leong, Jennifer; Coty, Patrick; Fiel, M Isabel; Chang, Charissa; Florman, Sander; Schiano, Thomas

    2014-11-01

    Most studies have shown that lamivudine (LAM) prophylaxis is sufficient to prevent hepatitis B virus (HBV) transmission in recipients of hepatitis B core antibody positive (HBcAb(+) ) allografts. However, de novo hepatitis B (DNHB) is known to occur in this patient population. Herein, we report a case series of four liver transplant recipients who developed DNHB after receiving HBcAb(+) allografts due to acquisition of LAM resistance mutations, suggesting that LAM prophylaxis may be suboptimal. A retrospective chart review was performed of all adult liver transplants performed at Mount Sinai from 2001 to 2010. A total of 79 patients received HBcAb(+) allografts for non-hepatitis B-related liver disease. Of these 79 recipients, four patients developed DNHB and were found to have documented LAM resistance. With the increasing use of HBcAb(+) donor livers, we suspect that there will also be a growing number of cases of DNHB due to acquisition of LAM resistance. We suggest that other agents, such as entecavir or tenofovir, be considered for use as prophylaxis in this patient population to decrease this risk. PMID:24107139

  8. Low proton conductance of plant cuticles and its relevance to the Acid-growth theory.

    PubMed

    Dreyer, S A; Seymour, V; Cleland, R E

    1981-09-01

    Evidence obtained on the relation between the pH of the medium and the growth of intact stem sections is compatible with the acid-growth theory only if the proton conductance of the cuticle is so low that the cuticle is an effective barrier to the entry or exit of protons from the tissue. By measuring the rate at which protons cross frozen-thawed epidermal strips of sunflower (Helianthus annuus L.) and soybean hypocotyls (Glycine max Morr.) and enzymically isolated cuticles of Berberis aquifolium Persh. and tomato (Lycopersicum esculentum Mill.) fruit, we have now demonstrated the low proton conductance of the cuticular layer. Unless the conductance is enhanced by abrasion of the cuticle or by removal of the cuticular waxes, proton movement into and out of a tissue across the cuticle will be significant only over long time periods. PMID:16661976

  9. Low Proton Conductance of Plant Cuticles and Its Relevance to the Acid-Growth Theory 1

    PubMed Central

    Dreyer, S. Ann; Seymour, Virginia; Cleland, Robert E.

    1981-01-01

    Evidence obtained on the relation between the pH of the medium and the growth of intact stem sections is compatible with the acid-growth theory only if the proton conductance of the cuticle is so low that the cuticle is an effective barrier to the entry or exit of protons from the tissue. By measuring the rate at which protons cross frozen-thawed epidermal strips of sunflower (Helianthus annuus L.) and soybean hypocotyls (Glycine max Morr.) and enzymically isolated cuticles of Berberis aquifolium Persh. and tomato (Lycopersicum esculentum Mill.) fruit, we have now demonstrated the low proton conductance of the cuticular layer. Unless the conductance is enhanced by abrasion of the cuticle or by removal of the cuticular waxes, proton movement into and out of a tissue across the cuticle will be significant only over long time periods. Images PMID:16661976

  10. Low proton conductance of plant cuticles and its relevance to the acid-growth theory

    SciTech Connect

    Dreyer, S.A.; Seymour, V.; Cleland, R.E.

    1981-09-01

    Evidence obtained on the relation between the pH of the medium and the growth of intact stem sections is compatible with the acid-growth theory only if the proton conductance of the cuticle is an effective barrier to the entry or exit of protons from the tissue. By measuring the rate at which protons cross frozen-thawed epidermal strips of sunflower (Helianthus annus L.) and soybean hypocotyls (Glycine max Morr.) and enzymically isolated cuticles of Berberis aquifolium Persh. and tomato (Lycopersicum esculentum Mill.) fruit, we have now demonstrated the low proton conductance of the cuticular layer. Unless the conductance is enhanced by abrasion of the cuticle or by removal of the cuticular waxes, proton movement into and out of a tissue across the cuticle will be significant only over long time periods.

  11. Eriophyoid mites from Qinghai Province, northwestern China with descriptions of nine new species (Acari, Eriophyoidea)

    PubMed Central

    Li, Hao-Sen; Xue, Xiao-Feng; Hong, Xiao-Yue

    2012-01-01

    Abstract Eriophyoid mites from Qinghai Province, northwestern China were studied herein. Up to now, only six species have been reported from Qinghai Province. In field surveys, 17 eriophyoid mite species were collected, among which nine species were found new to science. The new species and their host plants are listed as follows: Acaphyllisa tuberculumae sp. n. on Populus sp. (Salicaceae); Proiectus xiningensis sp. n. on Pinus sp. (Pinaceae); Phyllocoptes beishaniensis sp. n. on Spiraea mongolica Maxim. (Rosaceae); Tetra pruniana sp. n. on Prunus tomentosa Thunb. (Rosaceae) Rupr. (Berberidaceae); Tetra pyriana sp. n. on Pyrus calleryana Decne. (Rosaceae); Tetra simonia sp. n. on Populus simonii Carr. (Salicaceae); Diptacus berberinus sp. n. on Berberis amurensis Rupr. (Berberidaceae); Diptacus mengdaensis sp. n. on Lonicera elisae Franch. (Caprifoliaceae); Rhyncaphytoptus spinus sp. n. on Lonicera rupicola Hook. f. et Thoms. (Caprifoliaceae). Aculops ulmi Hong & Xue, 2005 was re-described. PMID:22679390

  12. Immunocytological localization of two enzymes involved in berberine biosynthesis.

    PubMed

    Bock, Armin; Wanner, Gerhard; Zenk, Meinhart H

    2002-11-01

    Using post-embedding immunogold techniques the cytological localization of the two branchpoint enzymes of isoquinoline biosynthesis, berberine bridge enzyme (BBE) and (S)-tetrahydroprotoberberine oxidase (STOX), was demonstrated. Electron-microscopic examination revealed their exclusive compartmentation within vesicles. After these vesicles have fused with the central vacuole, they release their contents, resulting in a characteristic electron-dense precipitate at the tonoplast. Vesicles of similar structure could be identified in young meristematic tissues of roots or shoots from different Berberis species and Papaver somniferum L. The appearance of electron-dense osmiophilic material is strictly correlated with the alkaloid content of the tissue. Immunocytological staining of P. somniferum tissue with antibodies directed against BBE led to a characteristic labeling of electron-dense aggregates in idioblasts that are not connected to the laticifer system. This localization demonstrates the strictly cytological separation of benzophenanthridine and morphine biosyntheses within this plant. PMID:12430014

  13. Historic Mining and Agriculture as Indicators of Occurrence and Abundance of Widespread Invasive Plant Species

    PubMed Central

    Calinger, Kellen; Calhoon, Elisabeth; Chang, Hsiao-chi; Whitacre, James; Wenzel, John; Comita, Liza; Queenborough, Simon

    2015-01-01

    Anthropogenic disturbances often change ecological communities and provide opportunities for non-native species invasion. Understanding the impacts of disturbances on species invasion is therefore crucial for invasive species management. We used generalized linear mixed effects models to explore the influence of land-use history and distance to roads on the occurrence and abundance of two invasive plant species (Rosa multiflora and Berberis thunbergii) in a 900-ha deciduous forest in the eastern U.S.A., the Powdermill Nature Reserve. Although much of the reserve has been continuously forested since at least 1939, aerial photos revealed a variety of land-uses since then including agriculture, mining, logging, and development. By 2008, both R. multiflora and B. thunbergii were widespread throughout the reserve (occurring in 24% and 13% of 4417 10-m diameter regularly-placed vegetation plots, respectively) with occurrence and abundance of each varying significantly with land-use history. Rosa multiflora was more likely to occur in historically farmed, mined, logged or developed plots than in plots that remained forested, (log odds of 1.8 to 3.0); Berberis thunbergii was more likely to occur in plots with agricultural, mining, or logging history than in plots without disturbance (log odds of 1.4 to 2.1). Mining, logging, and agriculture increased the probability that R. multiflora had >10% cover while only past agriculture was related to cover of B. thunbergii. Proximity to roads was positively correlated with the occurrence of R. multiflora (a 0.26 increase in the log odds for every 1-m closer) but not B. thunbergii, and roads had no impact on the abundance of either species. Our results indicated that a wide variety of disturbances may aid the introduction of invasive species into new habitats, while high-impact disturbances such as agriculture and mining increase the likelihood of high abundance post-introduction. PMID:26046534

  14. Historic Mining and Agriculture as Indicators of Occurrence and Abundance of Widespread Invasive Plant Species.

    PubMed

    Calinger, Kellen; Calhoon, Elisabeth; Chang, Hsiao-Chi; Whitacre, James; Wenzel, John; Comita, Liza; Queenborough, Simon

    2015-01-01

    Anthropogenic disturbances often change ecological communities and provide opportunities for non-native species invasion. Understanding the impacts of disturbances on species invasion is therefore crucial for invasive species management. We used generalized linear mixed effects models to explore the influence of land-use history and distance to roads on the occurrence and abundance of two invasive plant species (Rosa multiflora and Berberis thunbergii) in a 900-ha deciduous forest in the eastern U.S.A., the Powdermill Nature Reserve. Although much of the reserve has been continuously forested since at least 1939, aerial photos revealed a variety of land-uses since then including agriculture, mining, logging, and development. By 2008, both R. multiflora and B. thunbergii were widespread throughout the reserve (occurring in 24% and 13% of 4417 10-m diameter regularly-placed vegetation plots, respectively) with occurrence and abundance of each varying significantly with land-use history. Rosa multiflora was more likely to occur in historically farmed, mined, logged or developed plots than in plots that remained forested, (log odds of 1.8 to 3.0); Berberis thunbergii was more likely to occur in plots with agricultural, mining, or logging history than in plots without disturbance (log odds of 1.4 to 2.1). Mining, logging, and agriculture increased the probability that R. multiflora had >10% cover while only past agriculture was related to cover of B. thunbergii. Proximity to roads was positively correlated with the occurrence of R. multiflora (a 0.26 increase in the log odds for every 1-m closer) but not B. thunbergii, and roads had no impact on the abundance of either species. Our results indicated that a wide variety of disturbances may aid the introduction of invasive species into new habitats, while high-impact disturbances such as agriculture and mining increase the likelihood of high abundance post-introduction. PMID:26046534

  15. Induction ofEarly-Response GenesKC andJEbyMycobacterial Lipoarabinomannans: Regulation ofKC Expression inMurine Macrophages byLsh\\/Ity\\/Bcg (Candidate Nramp)

    Microsoft Academic Search

    TAMARA I. A. ROACH

    1994-01-01

    (LAM)to induce early gene (KCandJE)expression inmacrophages fromsusceptible (S)C57BL\\/lOScSn (Lshs) and congenic resistant (R)B10.L-Lshr micewas investigated. Stimulation with1.8,ugofarabinofuranosyl- terminated LAM (AraLAM) per ml resulted insimilar kinetics forKC or JE expression inS andR macrophages. However, whereasJE\\/glyceraldehyde 3-phosphate dehydrogenase (GAPDH)mRNA ratios remained equivalent, R macrophages consistently showedenhanced KC\\/GAPDHratios within 30to40minof stimulation comparedwithS macrophages. Significant differences inKC\\/GAPDHratios were observed throughout thepeakperiod (0.5 to6h)oftheKC responseandwithdosesofAraLAMranging

  16. Genes from Cellvibrio mixtus encoding. beta. -1,3 endoglucanase

    SciTech Connect

    Sakellaris, H.; Pemberton, J.M.; Manners, J.M. (Univ. of Queensland, St. Lucia (Australia))

    1990-10-01

    Two genes encoding {beta}-1,3 glucanase activity were cloned from the gram-negative soil bacterium Cellvibrio mixtus. The two clones, designated cwd (cell wall degradation) and lam (laminarin degradation), had distinct endonuclease restriction patterns and encoded enzymes with distinct substrate specificities. The 3.7-kilobase cwd insert encoded an enzyme which degraded yeast cell walls as well as the soluble {beta}-1,3 glucan laminarin and the insoluble {beta}-1,3 glucans zymosan and pachyman. The 1.8-kilobase lam insert encoded an enzyme which degraded lamarin only. Both enzymes degraded laminarin in an endohydrolytic manner to yield laminarobiose, laminarotriose, and laminarotetraose as major end products. Radiolabeled translation products of the cwd and lam transcripts were identified.

  17. Giant Bilateral Renal Angiomyolipomas and Lymphangioleiomyomatosis Presenting after Two Successive Pregnancies Successfully Treated with Surgery and Rapamycin

    PubMed Central

    Peces, Ramón; Cuesta-López, Emilio; Peces, Carlos; Selgas, Rafael

    2011-01-01

    We report the case of a 25-year-old woman who presented with abdominal and flank pain with two successive pregnancies and was diagnosed of giant bilateral renal AMLs and pulmonary LAM associated with TSC in the post-partum of her second pregnancy. This case illustrates that in women with TSC rapid growth from renal AMLs and development of LAM may occur with successive pregnancies. It also stresses the potential for preservation of renal function despite successive bilateral renal surgery of giant AMLs. Moreover, the treatment with a low-dose rapamycin may be an option for LAM treatment. Finally, a low-dose rapamycin may be considered as an adjuvant treatment together to kidney-sparing conservative surgery for renal AMLs. PMID:22125460

  18. Infrared, Raman and INS studies of poly-ethylene oxide oligomers

    NASA Astrophysics Data System (ADS)

    Migliardo, F.; Magazù, S.; Caccamo, M. T.

    2013-09-01

    In the present paper the experimental findings obtained by different spectroscopic techniques on water mixtures of a protein model system, i.e. PolyEthylene Oxide (PEO), are reported. By increasing the polymerization degree m, the D-LAM spectral contribution shows a behavior of the centre frequency and of linewidth which tends to reach a constant value. Also the intramolecular OH stretching contribution, by increasing m, shows a shift which tends to flatten for polymers with molecular weight higher than Mw = 600. These results can be connected with the oligomer-polymer transition, at m = 13. When in aqueous solutions the D-LAM frequency increases towards the value corresponding to that of the crystal phase; similarly one observes also a sharpening of the D-LAM spectral contribution. These evidences suggest the presence of a more ordered conformation of PEO in water in respect to the melt phase.

  19. Power-law scaling in protein synthesis of a stochastic regulon

    E-print Network

    Emily Chapman-McQuiston; Chuck Yeung; X. L. Wu

    2008-08-07

    We investigate the protein expression pattern of the lamB gene in Escherichia coli LE392. The gene product LamB is an important membrane protein for maltose transport into cells but it is also exploited by bacteriophage lambda for infection. Although our bacterial population is clonal, stochastic gene expression leads to a majority population with a large receptor number and a minority population with a small receptor number. We find that the LamB receptor distribution p(n) of the small-n population is scale invariant with the exponent depending on growth conditions. A heuristic model is proposed that relates the observed exponent to the protein production rate.

  20. Supramolecular Chemistry And Self-assembly Special Feature: Virus-assisted loading of polymer nanocontainer

    NASA Astrophysics Data System (ADS)

    Graff, Alexandra; Sauer, Marc; van Gelder, Patrick; Meier, Wolfgang

    2002-04-01

    We present a DNA-containing polymeric nanocontainer using the self-assembled superstructure of amphiphilic block copolymers in aqueous solutions. To demonstrate that DNA translocation is possible across a completely synthetic block copolymer membrane, we have used a phage transfection strategy as a DNA-transfer model system. For this purpose the bacterial channel forming protein LamB was reconstituted in ABA-triblock copolymer vesicles. The outer membrane protein LamB is a specific transporter for maltodextrins but also serves as a receptor for phage to trigger the ejection of phage DNA. We demonstrate that the functionality of the LamB protein is fully preserved despite the artificial surrounding. This leads to a type of polymeric vehicle for DNA that could be useful for gene therapy.

  1. A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis

    PubMed Central

    Hamasur, Beston; Bruchfeld, Judith; van Helden, Paul; Källenius, Gunilla

    2015-01-01

    We have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration step. We have now developed an easy to perform test based on a magnetic immunoassay platform, utilizing high avidity monoclonal antibodies for the detection of LAM in urine. With this method the analytical sensitivity of the assay was increased 50-100-fold compared to conventional ELISA. In a pilot study of HIV-negative patients with microbiologically verified TB (n=17) and healthy controls (n=22) the sensitivity of the test was 82% and the specificity 100%. This is in stark positive contrast to a range of studies using available commercial tests with polyclonal anti-LAM Abs where the sensitivity of the tests in HIV-negative TB patients was very low. PMID:25905641

  2. Ethnoveterinary study of medicinal plants in Malakand Valley, District Dir (Lower), Khyber Pakhtunkhwa, Pakistan

    PubMed Central

    2014-01-01

    Background The Malakand valley of District Dir (Lower) is endowed with a diverse plant wealth. Ethnoveterinary knowledge provides the traditional medicines of livestock that are cheaper than standard treatment and are easily accessible. Methods The present study was conducted to document the traditional knowledge of ethnoveterinary practices in Malakand valley, District Dir (Lower). Data was collected from February 2012 to January 2013 by interviewing 120 inhabitants through semi-structured questionnaires. Different questions were asked to collect appropriate data regarding the use of plants for livestock treatment. Direct matrix ranking (DMR) was carried out to find out the use diversity of medicinal plants. Findings A total of 28 plants belonging to 23 families were collected and identified for the treatment of livestock in the study area. Majority of the plants were collected from wild (68%) habitat and very few from cultivated sources. The leaves (28%) were identified as the major plant part for herbal preparations. The most frequent ailments of livestocks in the study area were stomach disorders and Charmaikh (local disease name). Various ingredients were used along with ethnoveterinary medicines i.e. sugar, flour, milk etc. The elder people of the village had a rich knowledge as compared to the young generation. According to DMR output, Monotheca buxifolia ranked first and Dalbergia sisso and Melia azedarach ranked second due to high multipurpose uses among all species and was found most threatened in the study area. Conclusion It has been concluded that the native of the region heavily dependent on medicinal plants for the treatment of variety of livestock ailments. Traditional knowledge always provides a baseline for further phytochemical and pharmacological investigation. The documentation of the ethnoveterinary practices in study area was necessary before this precious knowledge is lost forever due to rapid socioeconomic, environmental and technological changes. PMID:24580769

  3. Point-of-Care Lateral Flow Assays for Tuberculosis and Cryptococcal Antigenuria Predict Death in HIV Infected Adults in Uganda

    PubMed Central

    Manabe, Yukari C.; Nonyane, Bareng A. S.; Nakiyingi, Lydia; Mbabazi, Olive; Lubega, Gloria; Shah, Maunank; Moulton, Lawrence H.; Joloba, Moses; Ellner, Jerrold; Dorman, Susan E.

    2014-01-01

    Background Mortality in hospitalized, febrile patients in Sub-Saharan Africa is high due to HIV-infected, severely immunosuppressed patients with opportunistic co-infection, particularly disseminated tuberculosis (TB) and cryptococcal disease. We sought to determine if a positive lateral flow assay (LFA) result for urine lipoarabinomannan (LAM) and cryptococcal antigenuria was associated with mortality. Methods 351 hospitalized, HIV-positive adults with symptoms consistent with TB and who were able to provide both urine and sputum specimens were prospectively enrolled at Mulago National Referral Hospital in Uganda as part of a prospective accuracy evaluation of the lateral flow Determine TB LAM test. Stored frozen urine was retrospectively tested for cryptococcal antigen (CRAG) using the LFA. We fitted a multinomial logistic regression model to analyze factors associated with death within 2 months after initial presentation. Results The median CD4 of the participants was 57 (IQR: 14–179) cells/µl and 41% (145) were microbiologically confirmed TB cases. LAM LFA was positive in 38% (134), 7% (25) were CRAG positive, and 43% (151) were positive for either test in urine. Overall, 21% (75) died within the first 2 months, and a total of 32% (114) were confirmed dead by 6 months. At 2 months, 30% of LAM or CRAG positive patients were confirmed dead compared to 15.0% of those who were negative. In an adjusted model, LAM or CRAG positive results were associated with an increased risk of death (RRR 2.29, 95% CI: 1.29, 4.05; P?=?0.005). Conclusions In hospitalized HIV-infected patients, LAM or CRAG LFA positivity was associated with subsequent death within 2 months. Further studies are warranted to examine the impact of POC diagnostic ‘test and treat’ approach on patient-centered outcomes. PMID:25000489

  4. Estrogen receptor immunocytochemical assay (ERICA) and laminin detection in 130 breast carcinomas and computerized (Samba 200) multiparametric quantitative analysis on tissue sections.

    PubMed

    Charpin, C; Martin, P M; Lissitzky, J C; Jacquemier, J; Kopp, F; Pourreau-Schneider, N; Lavaut, M N; Toga, M

    1986-01-01

    An estrogen receptor immunocytochemical assay (ER-ICA) was applied to 130 malignant breast carcinomas and the results were compared to those of steroid binding assays performed on cytosol extracts of the same tumors. Also laminin (lam) distribution was studied in the same tumors. A semi quantitative analysis and a computerized image analysis system (SAMBA 200 TITN) were used to evaluate the positive ER and lam immunostaining. Positive ER immunostaining was always located in the nucleus of tumor cells and of normal cells in adjacent breast tissue. When immunohistochemical staining was correlated to biochemical assay there was a 88% correlation staining intensity and percentage of positive cells significantly increased (p less than 0.01) with cytosolic ER levels. Lam was observed within vascular and epithelial basement membranes (BMs). Lam staining displayed a continuous linear pattern in intraductal carcinomas or was heterogeneously distributed with a discontinuous linear pattern in invasive carcinomas. No intracellular lam staining was detected. In tumors, laminin immunostaining revealed often multilayered BMs and abnormal multilayered BMs in blood vessels in the tumor stroma. These results indicated that (ER-ICA) is to date the most reliable histochemical method for ER detection and correlated in 88% of the cases with ER biochemical assay ER-ICA provides additional information for heterogeneous ER distribution within tumors ER-ICA as a qualitative method is unable to replace the quantitative ER determination obtained with biochemical assay ER-ICA based on ER antigenic site detection is complementary to biochemical assay based on ER functional site determination laminin immunostaing constitutes a new approach to the heterogeneous BM changes occurring in carcinomas, and permits a better understanding of cell diffusion processes and of stroma-tumor cells interactions: the consistent extracellular lam distribution in contact with the stroma, indicates that the latter plays an important role in the assembly of BM components the SAMBA 200 permits a reliable accurate evaluation of the percentage of the immunostained cells and surfaces. PMID:3567371

  5. Effects of short-term lifestyle activity modification on adiponectin mRNA expression and plasma concentrations.

    PubMed

    Moghadasi, Mehrzad; Mohebbi, Hamid; Rahmani-Nia, Farhad; Hassan-Nia, Sadegh; Noroozi, Hamid

    2013-01-01

    Adiponectin mRNA and plasma concentrations were reduced in obesity. Exercise training may reduce the adipose tissue (AT), although it is not well known, whether exercise - induced change in AT, increases adiponectin mRNA expression and plasma concentrations or not. Therefore, the purpose of this study was to examine the effects of short-term lifestyle activity modification (LAM) on adiponectin mRNA and plasma concentrations. Sixteen obese and overweight middle-aged men (age, 35-50 years) with type 2 diabetes participated in this study. The subjects were randomly assigned to LAM group (n=8) or control group (n=8). The subjects in LAM group walked two miles in 30 min on a treadmill on 4 days per week for 12 weeks according to the guidelines of the Centers for Disease Control and Prevention and American College of Sports Medicine. The results showed that body mass, body mass index, central visceral adipose tissue and subcutaneous adipose tissue volume and hip and thigh subcutaneous adipose tissue (hip and thigh SAT) volume were decreased in the LAM group compared to the control group (P<0.05). Adiponectin mRNA in abdominal and gluteal subcutaneous AT were increased significantly in the LAM group compared to the control group (P<0.05), while plasma adiponectin concentrations, hs-CRP and insulin resistance did not change significantly. In conclusion, adiponectin mRNA levels increase after 12 weeks of LAM; however, plasma adiponectin levels were not affected by this protocol in obese and overweight middle-aged men with type 2 diabetes. PMID:23834543

  6. Strain classification of Mycobacterium tuberculosis isolates in Brazil based on genotypes obtained by spoligotyping, mycobacterial interspersed repetitive unit typing and the presence of large sequence and single nucleotide polymorphism.

    PubMed

    Vasconcellos, Sidra E G; Acosta, Chyntia Carolina; Gomes, Lia Lima; Conceição, Emilyn Costa; Lima, Karla Valéria; de Araujo, Marcelo Ivens; Leite, Maria de Lourdes; Tannure, Flávio; Caldas, Paulo Cesar de Souza; Gomes, Harrison M; Santos, Adalberto Rezende; Gomgnimbou, Michel K; Sola, Christophe; Couvin, David; Rastogi, Nalin; Boechat, Neio; Suffys, Philip Noel

    2014-01-01

    Rio de Janeiro is endemic for tuberculosis (TB) and presents the second largest prevalence of the disease in Brazil. Here, we present the bacterial population structure of 218 isolates of Mycobacterium tuberculosis, derived from 186 patients that were diagnosed between January 2008 and December 2009. Genotypes were generated by means of spoligotyping, 24 MIRU-VNTR typing and presence of fbpC103, RDRio and RD174. The results confirmed earlier data that predominant genotypes in Rio de Janeiro are those of the Euro American Lineages (99%). However, we observed differences between the classification by spoligotyping when comparing to that of 24 MIRU-VNTR typing, being respectively 43.6% vs. 62.4% of LAM, 34.9% vs. 9.6% of T and 18.3% vs. 21.5% of Haarlem. Among isolates classified as LAM by MIRU typing, 28.0% did not present the characteristic spoligotype profile with absence of spacers 21 to 24 and 32 to 36 and we designated these conveniently as "LAM-like", 79.3% of these presenting the LAM-specific SNP fbpC103. The frequency of RDRio and RD174 in the LAM strains, as defined both by spoligotyping and 24 MIRU-VNTR loci, were respectively 11% and 15.4%, demonstrating that RD174 is not always a marker for LAM/RDRio strains. We conclude that, although spoligotyping alone is a tool for classification of strains of the Euro-American lineage, when combined with MIRU-VNTRs, SNPs and RD typing, it leads to a much better understanding of the bacterial population structure and phylogenetic relationships among strains of M. tuberculosis in regions with high incidence of TB. PMID:25314118

  7. The x-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale

    Microsoft Academic Search

    Bryan W. Lepore; Frank J. Ruzicka; Perry A. Frey; Dagmar Ringe

    2005-01-01

    The x-ray crystal structure of the pyridoxal-5'-phosphate (PLP), S-adenosyl-l-methionine (SAM), and [4Fe-4S]-dependent lysine-2,3-aminomutase (LAM) of Clostridium subterminale has been solved to 2.1-Å resolution by single-wavelength anomalous dispersion methods on a l-selenomethionine-substituted complex of LAM with [4Fe-4S]2+, PLP, SAM, and l-alpha-lysine, a very close analog of the active Michaelis complex. The unit cell contains a dimer of hydrogen-bonded, domain-swapped dimers, the

  8. Management of refractory chylothorax in pulmonary lymphangioleiomyomatosis

    PubMed Central

    Ellender, Claire M; Williams, Trevor J; Gooi, Julian; Snell, Gregory I; Whitford, Helen M

    2015-01-01

    This case reports the successful management of chylothorax in a non-transplanted patient with pulmonary lymphangioleiomyomatosis (pLAM). Prolonged initial therapy failed, including total parenteral nutrition, pleural drainage, surgical pleurodesis, and pleurectomy. Commencement of sirolimus 2?mg daily (2?mg alternating days had failed) led to resolution of chylothorax after 20 days. Discontinuation of sirolimus for abdominal surgery led to recurrence of the chylothorax. Reinstitution of sirolimus led to rapid resolution of the effusion, stabilization of lung function, and there has been no recurrence in the ensuing 4 years. We conclude that sirolimus should be considered in the management of pLAM-related chylothorax, perhaps before surgical intervention.

  9. Induction of Inducible Nitric Oxide Synthase-NOz by Lipoarabinomannan of Mycobacterium tuberculosis Is Mediated by MEK1ERK, MKK7JNK, and NF-kB Signaling Pathways

    Microsoft Academic Search

    EDWARD D. CHAN; KRISTIN R. MORRIS; JOHN T. BELISLE; PRESTON HILL; LINDA K. REMIGIO; PATRICK J. BRENNAN; DAVID W. H. RICHES

    2010-01-01

    gamma interferon (IFN-g)-ManLAM-induced iNOS protein and NO2 2 expression in mouse macrophages. Transient cotransfection of dominant-negative protein mutants of the c-Jun NH2-terminal kinase (JNK) pathway revealed that the MAP kinase kinase 7 (MKK7)-JNK cascade also mediated IFN-g-ManLAM induction of iNOS promoter activity whereas MKK4 did not. Overexpression of null mutant IkBa, a potent inhibitor of NF-kB activation, confirmed that the

  10. Why Digital Data Collections Are Important

    ERIC Educational Resources Information Center

    Mitchell, Erik T.

    2012-01-01

    The silo is a well-worn metaphor in information systems used to illustrate separateness, isolation, and lack of connectivity. Through the many iterations of system development, libraries, archives, and museums (LAMs) have sought to avoid silos and find the sweet spot between interface design and metadata interoperability. This effort is being…

  11. ‘Liberty’ Dry-Fleshed Sweetpotato

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The sweetpotato [Ipomoea batatas (L.) Lam.] cultivar, ‘Liberty’ was jointly developed by the U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS), and Clemson University, South Carolina Agriculture and Forestry Research System. This cultivar is a dry-fleshed type with attracti...

  12. ‘Charleston Scarlet’ Sweetpotato

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The sweetpotato [Ipomoea batatas (L.) Lam.] cultivar, ‘Charleston Scarlet’ was developed by the U.S. Department of Agriculture, Agricultural Research Service, Charleston, SC. ‘Charleston Scarlet’ produces orange-fleshed, sweet storage roots with attractive scarlet-colored skin (periderm). Vine gro...

  13. Basic Protein Identification of 1D SDS PAGE gel bands Just want to know what protein that band in my gel is? Then this is the service for you!

    E-print Network

    Lee, Daeyeol

    Basic Protein Identification of 1D SDS PAGE gel bands Just want to know what protein.lam@yale.edu for Profit pricing. 1 2 3 4 Protein ID'ed Selected Gel Bands 1 2 3 4 #12; that band in my gel is? Then this is the service for you! Samples need to be... In a coomassie blue stained

  14. Ulrich L. Rohde Ajay K. Poddar http://piezo.nbu.edu.cn

    E-print Network

    Wang, Ji

    /GPRS/EDGE Radio Front-ends AERO42xx," http://www.nxp.com/acrobat_download/literature/9397/75015986.pdf. 3. M MEMS (RF MEMS) SiTime MEMS (ABI) Doug McEuen " " #12;17 1. C.S. Lam, "A Review of the Recent

  15. proteinsSTRUCTURE O FUNCTION O BIOINFORMATICS Solid-state NMR and simulation studies of

    E-print Network

    Watts, Anthony

    II N-terminus interaction with lipid bilayers Yuen Han Lam,1 Andrew Hung,2 Raymond S. Norton,3 of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia 4 School of Chemistry, Bio21, significant changes in the distan- ces between the labeled amino acid pairs were observed, suggesting changes

  16. Micro-Interactions with NFC-Enabled Mobile Phones

    E-print Network

    Pratt, Vaughan

    Micro-Interactions with NFC-Enabled Mobile Phones Ben Dodson Monica S. Lam Computer Science- neously. For example, a mobile payment using near-field communication (NFC) is a micro-interaction. The arrival of NFC on smart phones makes possible a wide array of applications using micro-interactions, from

  17. Targeting Adenovirus to the Serotype 3 Receptor Increases Gene Transfer Efficiency to Ovarian Cancer Cells1

    E-print Network

    Hemminki, Akseli

    , Alabama 35294 [G. V. M., V. K.] ABSTRACT Gene delivery efficiency in clinical cancer gene therapy trials Cancer Cells1 Anna Kanerva, Galina V. Mikheeva, Victor Krasnykh, Candace J. Coolidge, John T. Lam Hemminki, and David T. Curiel2 Division of Human Gene Therapy, Departments of Medicine, Pathology

  18. Forage Intake and Digestion by Cattle Grazing Midgrass Prairie Rangeland or Sideoats Grama\\/Sweetclover Pasture'12

    Microsoft Academic Search

    Stacey A. Guntel; F. Ted McCollum; Robert L. Gilled; Les J. KryslG

    In mid-May, beef cattle fitted with esophageal (four steers\\/pasture) or ruminal and duodenal cannulas (six heiferdpasture; 274 f 6 kg BW f SEI grazed midgrass prairie (excellent range condition; PRAIRIE) or a seeded mixture of sideoats grama (48% of pasture DM) and sweetclover (6% of pasture DM; Bouteloua curtipendula (Michx.) Torr.\\/ Melilotus oficinalis (L.) Lam.; PASTURE). Masticate NDF, ADF, and

  19. Cornell University, Office of Sponsored Programs Awards Received in February 2003

    E-print Network

    Danforth, Bryan Nicholas

    PUBLISHING PROJECT $39,215 LAM 42488 BRUTSAERT, WILFRIED H CEE JOHNS HOPKINS U SENSITIVITY OF COUPLED LAND-ATMOSPHERIC BOUNDARY LAYER DYNAMICS $73,179 DBW 37456 BRUYERE, SUSANNE M ILR-EXT ROCKEFELLER TRENDS AND BEST PRACTICES DEVELOPMENT OF METAL-BASED CATALYSTS FOR THE SYNTHESIS OF POLYMERS WITH REDUCED ENVIRONMENTAL IMPACT $125

  20. Analysis of Stripping to Quasibound Levels in Sc-41

    E-print Network

    Youngblood, David H.; Kozub, R. L.; Kenefick, R. A.; Hiebert, John C.

    1970-01-01

    . Duhm, and R. Stock, Phys. Letters 18, 61 {1965}. 4D. H. Youngblood, R. L. Kozub, R. A. Kenefick, andJ. C. Hiebert, Nucl. Phys. A143, 512 (1970). D. A. Gedcke, S. T. Lam, S. M. Tang, G. M. Stinson,J. T. Sample, T. B.Grandy, W. J. McDonald, W, K. 488...

  1. Analyze Influenza Virus Sequences Using Binary Encoding Approach

    E-print Network

    Boley, Daniel

    Analyze Influenza Virus Sequences Using Binary Encoding Approach Ham Ching Lam , Srinand Sreevatsan and Daniel Boley Abstract Capturing mutation patterns of each individual influenza virus sequence is often the influenza genetic sequences to a binary strings and form a binary sequence alignment matrix and then apply

  2. Foliage of Oaks Grown Under Elevated CO 2 Reduces Performance of Antheraea polyphemus (Lepidoptera: Saturniidae)

    Microsoft Academic Search

    Rachel G. Knepp; Jason G. Hamilton; Arthur R. Zangerl; May R. Berenbaum; Evan H. DeLucia

    2007-01-01

    To understand how the increase in atmospheric CO2 from human activity may affect leaf damage by forest insects, we examined host plant preference and larval performance of a generalist herbivore, Antheraea polyphemus Cram., that consumed foliage developed under ambient or elevated CO2. Larvae were fed leaves from Quercus alba L. and Quercus velutina Lam. grown under ambient or plus 200

  3. Formative and proliferative cell divisions, cell differentiation, and developmental changes in the meristem of Azolla roots

    Microsoft Academic Search

    B. E. S. Gunning; J. E. Hughes; A. R. Hardham

    1978-01-01

    The root of the water fern Azolla is a compact higher-plant organ, advantageous for studies of cell division, cell differentiation, and morphogenesis. The cell complement of A. filiculoides Lam. and A. pinnata R.Br. roots is described, and the lineages of the cell types, all derived ultimately from a tetrahedral apical cell, are characterised in terms of sites and planes of

  4. Estimation of stress in the presence of closely located elastic inclusions: A numerical study

    E-print Network

    Kang, Hyeonbae

    of linear isotropic elasticity. The problem of estimating the stress attracts much attention latelyEstimation of stress in the presence of closely located elastic inclusions: A numerical study inside an elastic body, it is expected that the stress (the gradient of solutions to the Lam´e system

  5. HPC\\/VORX: a local area multicomputer system

    Microsoft Academic Search

    R. D. Gaglianello; B. S. Robinson; T. L. Lindstrom; E. E. Sampieri

    1989-01-01

    A system is described that combines the major strengths of local area networks and multicomputers, namely resource sharing over geographically significant distances and small communication latencies. The result is a new architecture called a local area multicomputer (LAM). The system that is described attempts to solve the seemingly incompatible needs of the two application domains by utilizing a high-capacity, low-latency

  6. The Future Challenge Our path seems clear. We have come to under-

    E-print Network

    Galan, Jorge E

    . Costerton, G. G. Geesey, K.-J. Cheng, Sci. Am. 238, 86 (February 1978). 2. J. R. Lawrence, D. R. Korber, B, K. Lam, B. D. Ellis, J. W. Costerton, Am. Soc. Artif. Intern. Organs J. 38, 174 (1992). 8. D. W. Lambe Jr., K. P. Fergeson, K. J. Mayberry-Carson, B. Tober-Meyer, J. W. Costerton, Clin. Orthop. 266

  7. An improved method of DNA extraction from Diaphorina citri for HLB detection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Huanglongbing (HLB) is a devastating disease of citrus that is transmitted by two citrus psyllids. Diaphorina citri transmits Candidatus Liberibacter asiaticus (Las) and Ca. L. americanus (Lam), and Trioza erytreae transmits Ca. L. africanus (Laf). Ca. Liberibacter species can be detected in DNA ex...

  8. Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus

    PubMed Central

    Tschiggerl, Helga; Breitwieser, Andreas; de Roo, Guy; Verwoerd, Theo; Sc?affer, Christina; Sleytr, Uwe B.

    2015-01-01

    A fusion protein based on the S-layer protein SbpA from Bacillus sphaericus CCM 2177 and the enzyme laminarinase (LamA) from Pyrococcus furiosus was designed and overexpressed in Escherichia coli. Due to the construction principle, the S-layer fusion protein fully retained the self-assembly capability of the S-layer moiety, while the catalytic domain of LamA remained exposed at the outer surface of the formed protein lattice. The enzyme activity of the S-layer fusion protein monolayer obtained upon recrystallization on silicon wafers, glass slides and different types of polymer membranes was determined colorimetrically and related to the activity of sole LamA that has been immobilized with conventional techniques. LamA aligned within the S-layer fusion protein lattice in a periodic and orientated fashion catalyzed twice the glucose release from the laminarin polysaccharide substrate in comparison to the randomly immobilized enzyme. In combination with the good shelf-life and the high resistance towards temperature and diverse chemicals, these novel composites are regarded a promising approach for site-directed enzyme immobilization. PMID:18035441

  9. Mosquito larvicidal activity of oleic and linoleic acids isolated from Citrullus colocynthis (Linn.) Schrad

    Microsoft Academic Search

    A. Abdul Rahuman; P. Venkatesan; Geetha Gopalakrishnan

    2008-01-01

    In mosquito control programs, botanical origin may have the potential to be used successfully as larvicides. The larvicidal\\u000a activity of crude acetone, hexane, ethyl acetate, methanol, and petroleum ether extracts of the leaf of Centella asiatica Linn., Datura metal Linn., Mukia scabrella Arn., Toddalia asiatica (Linn.) Lam, extracts of whole plant of Citrullus colocynthis (Linn.) Schrad, and Sphaeranthus indicus Linn.

  10. UBC Social Ecological Economic Development Studies (SEEDS) Student Report An Investigation into the Feasibility of Rain Gardens

    E-print Network

    into the Feasibility of Rain Gardens as a Stormwater Management Solution Alfred Lam Raymond Tam Robert Young Sangpil Investigation into the Feasibility of Rain Gardens as a Stormwater Management Solution Applied Science 262 discharging into sensitive waterways, UBC is planning to include two rain-gardens in the construction

  11. Int. J. Plant Sci. 163(1):5165. 2002. 2002 by The University of Chicago. All rights reserved.

    E-print Network

    Olson, Mark

    included Moringa oleifera Lam. and Carica papaya L. as representatives of their respective families of the four genera of Caricaceae, with two species of Moringa used as an outgroup. In the tree resulting from that the magnitude of differences between Moringa species would, in other plant groups, be used to delineate family

  12. Nitrotyrosine formation after activation of murine macrophages with mycobacteria and mycobacterial lipoarabinomannan

    PubMed Central

    Venkataprasad, N; Riveros-Moreno, V; Sosnowska, D; Moreno, C

    1999-01-01

    Murine peritoneal macrophages, elicited with thioglycollate, were stimulated in vitro with lipopolysaccharide (LPS). The production of nitrite, superoxide anion (SOA), and the accumulation of nitrotyrosine in the cells increased after treatment, and all were inhibitable by the NO synthase inhibitor NG-monomethyl-l-arginine monoacetate (l-NMMA). This effect suggests a direct correlation between the accumulation of those metabolites and NO synthase activity. Lipoarabinomannan (LAM) purified from Mycobacterium tuberculosis was added to peritoneal macrophages in the presence of interferon-gamma (IFN-?); the cells produced nitrite and SOA, both inhibitable by l-NMMA. There was, as well, accumulation of nitrotyrosine in the macrophage proteins. Strikingly, the amount of nitrotyrosine measured after LAM plus IFN-?, or LAM plus the low molecular weight adjuvant glutamylmuramyl dipeptide (GMDP), increased significantly in the presence of l-NMMA. These results suggest that murine macrophages, upon LAM stimulation, might generate reactive nitrogen metabolites by a route other than NO synthase. Nitrotyrosine accumulation after infection of macrophages in vitro, with either live bacille Calmette–Guérin (BCG) or live M. tuberculosis, in the presence or absence of IFN-?, showed no correlation with nitrite production, suggesting a low superoxide production. PMID:10337018

  13. 13th International Conference on Liquid and Amorphous Metals

    Microsoft Academic Search

    Pjotr Popel; Boris Gelchinskii; Valeriy Sidorov; Leonid Son; Alexandre Sabirzjanov

    2007-01-01

    The state of the art in the field of liquid and amorphous metals and alloys is regularly updated through two series of complementary international conferences, the LAM (Liquid and Amorphous Metals) and the RQ (Rapidly Quenched Materials). The first series of the conferences started as LM-1 in 1966 at Brookhaven for the basic understanding of liquid metals. The subsequent LM

  14. An experimental investigation of pulsed laser-assisted machining of AISI 52100 steel

    NASA Astrophysics Data System (ADS)

    Panjehpour, Afshin; Soleymani Yazdi, Mohammad R.; Shoja-Razavi, Reza

    2014-11-01

    Grinding and hard turning are widely used for machining of hardened bearing steel parts. Laser-assisted machining (LAM) has emerged as an efficient alternative to grinding and hard turning for hardened steel parts. In most cases, continuous-wave lasers were used as a heat source to cause localized heating prior to material removal by a cutting tool. In this study, an experimental investigation of pulsed laser-assisted machining of AISI 52100 bearing steel was conducted. The effects of process parameters (i.e., laser mean power, pulse frequency, pulse energy, cutting speed and feed rate) on state variables (i.e., material removal temperature, specific cutting energy, surface roughness, microstructure, tool wear and chip formation) were investigated. At laser mean power of 425 W with frequency of 120 Hz and cutting speed of 70 m/min, the benefit of LAM was shown by 25% decrease in specific cutting energy and 18% improvement in surface roughness, as compared to those of the conventional machining. It was shown that at constant laser power, the increase of laser pulse energy causes the rapid increase in tool wear rate. Pulsed laser allowed efficient control of surface temperature and heat penetration in material removal region. Examination of the machined subsurface microstructure and microhardness profiles showed no change under LAM and conventional machining. Continuous chips with more uniform plastic deformation were produced in LAM.

  15. Hydroponic plate\\/fabric\\/grass system for treatment of aquacultural wastewater

    Microsoft Academic Search

    Jimming Pan; Huawei Sun; Andre Nduwimana; Yuanyuan Wang; Gendi Zhou; Yibin Ying; Ruihong Zhang

    2007-01-01

    Hydroponic plants can efficiently absorb and uptake soluble compounds in wastewater but they have low abilities to remove suspended solids due to the lack of culture media to trap solids. This paper presented an improved hydroponic method for effective treatment of the wastewater from the backwash of recirculating aquacultural systems. The ryegrass (Lolium perenne Lam) was cultured with improved media

  16. Potential role of Thermus thermophilus and T. oshimai in high rates of nitrous oxide (N2O) production in $80 C hot

    E-print Network

    Ahmad, Sajjad

    ) production in $80 °C hot springs in the US Great Basin B. P. HEDLUND,1 A. I. MCDONALD,1 J. LAM,1 J. A Boiling Spring (GBS) in the US Great Basin depended on tem- perature, with the highest flux, 67.8 ± 2 denitrification pathway in these organisms and on the fate of biogenic N2O in geothermal environments. Received 21

  17. Data Analysis and Investigation of Self-Similarity in Oceanographic Sediment Data

    E-print Network

    Haddadi, Hamed

    commercially [9]. 3. Methodology Basic analysis on the time series and frequency domain using Fast FourierData Analysis and Investigation of Self-Similarity in Oceanographic Sediment Data Lam Ling Shum obtained from the WaveNet project. In particular we would like to search for any deterministic trends

  18. Manfred Knebusch Specialization of Quadratic

    E-print Network

    #= 2. It served me in the first place as the foundation for a theory of generic splitting of quadratic) generic splitting of central simple algebras and reductive algebraic groups, parallel to the theory during a talk by T.Y. Lam on field invariants from the theory of quadratic forms. 1 It is -- poetic

  19. Designing for Inquiry-Based Learning with the Learning Activity Management System

    ERIC Educational Resources Information Center

    Levy, P.; Aiyegbayo, O.; Little, S.

    2009-01-01

    This paper explores the relationship between practitioners' pedagogical purposes, values and practices in designing for inquiry-based learning in higher education, and the affordances of the Learning Activity Management System (LAMS) as a tool for creating learning designs in this context. Using a qualitative research methodology, variation was…

  20. To appear in the SIGGRAPH conference proceedings A Practical Model for Subsurface Light Transport

    E-print Network

    Stanford University

    scat- tering, with any subsurface scattering approximated by a Lam- bertian component. An exception is the model by Hanrahan and Krueger [10] which includes an analytic expression for single scat- tering] used scattering functions to simulate subsurface scat- tering. These approaches, while capable

  1. adfa, p. 1, 2011. Springer-Verlag Berlin Heidelberg 2011

    E-print Network

    Hammerton, James

    , School of Computer Science and Statistics, Trinity College Dublin, Ireland {Athanasios.Staikopoulos, Ian environments such as LAMS [3] have investigated the pedagogic benefits of learning activities adaptive and Personalised Learning Activities on the Web Athanasios Staikopoulos, Ian O'Keeffe, Rachael

  2. Phytotoxicity of Constituents of Glandular Trichomes and the Leaf Surface of Camphorweed, Heterotheca subaxillaris

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Camphorweed, Heterotheca subaxillaris (Lam.) Britt. & Rusby has a camphor-like odor, and its leaf surfaces contain glandular trichomes of the type shown to contain high levels of isoprenoids in other species. Phytotoxic calamenene-type sesquiterpenes (1-4, 8-10), borneol (11) and methylated flavone...

  3. Invariant integration over the orthogonal group

    NASA Astrophysics Data System (ADS)

    Braun, Daniel

    2006-11-01

    I adapt a recently introduced method for integrating over the unitary group (Aubert S and Lam C S 2003 J. Math. Phys. 44 6112-31) to the orthogonal group. I derive explicit formulae for a number of one, two and three-vector integrals, as well as recursion formulae for more complicated cases.

  4. On the Thrashing Cusp in Slotted Aloha Systems

    Microsoft Academic Search

    YOSHIKUNI ONOZATO; SHOICHI NOGUCHI

    1985-01-01

    We develop a new tool for performance evaluation of a multiaccess communication system. We give an explicit detailed analytic description of a cusp catastrophe in a computer communication system. An approximate model is formulated for a slotted Aloha system assuming a single buffer, based on the Markovian model studied by Lam [2] and Carleial [3]. Next, we outline the research

  5. An Insight-Based Longitudinal Study of Visual Analytics

    E-print Network

    of the study was to gain basic understanding into the visual analytic process. The primary research questionsAn Insight-Based Longitudinal Study of Visual Analytics Purvi Saraiya, Chris North, Vy Lam, and Karen A. Duca Abstract--Visualization tools are typically evaluated in controlled studies that observe

  6. E-print Network

    Stallard, Tom

    detection of Rego et al. (1999a), as well the mechanism proposed by Hill (1979) to explain how Jupiter balance there (Lam et al. 1997, Miller et al. 1997, Rego et al. 2000). Results from the Galileo space (Rego et al. 1999a), has renewed interest in models proposed over 20 years ago to explain the coupling

  7. Traitement de l’information à contenu émotionnel et représentation de soi chez des patients bipolaires de type I après traitement combiné médicamenteux et comportemental-cognitif

    Microsoft Academic Search

    C. Mirabel-Sarron; I. Urdapilleta; J.-D. Guelfi; F. Rouillon

    2009-01-01

    ObjectivesBipolar disorder currently affects around 1 % of the general population. Despite mood stabilizers, 37 % of patients continue to present mood fluctuations. Moreover, psychotherapeutic approaches, together with prophylactic treatment, have been developed over the past 10 years, including the behavioural and cognitive approach. Lam's behavioural model has thus become a major reference. Bipolar patients who have undergone cognitive behavioural therapy (CBT) based

  8. The Fourth International Conference on Advances in Steel Structures 13-15 June 2005, Shanghai, China

    E-print Network

    barrier against wide range use of FRP composites in structural strengthening and repair applications [Ref Strengthened Using FRP Composites, Construction and Building Materials, 18, 9-19. 2. Teng, J.G., Chen, J.F., Smith, S.T. and Lam, L. (2001), FRP ­ Strengthened RC Structures, John Wiley & Sons, Ltd, West Sussex

  9. The Fourth International Conference on Advances in Steel Structures 13-15 June 2005, Shanghai, China

    E-print Network

    , there is a need to develop appropriate design guidelines for CFRP strengthened steel structures [Ref. 4]. Studied bonded FRP systems for strengthening concrete structures. ACI Committee 440. Technical Committee Documents, ACI, 2002. 2. Teng, J.G., Chen, J.F., Smith, S.T. and Lam, L. (2002). FRP strengthened RC

  10. Allelopathic effect of parthenium ( Parthenium hysterophorus L.) extract and residue on some agronomic crops and weeds

    Microsoft Academic Search

    Wondimagegnehu Mersie; Megh Singh

    1987-01-01

    Allelopathic effects of entire shoot extract, plant part extracts, and shoot residue of parthenium (Parthenium hysterophorus L.) on corn (Zea mays L.), ryegrass (Lolium multiflorum Lam.), wheat (Triticum aestivum L.), velvetleaf (Abutilon theophrasti Medik.), and soybean [Glycine max (L) Merr.] growth were examined. Parthenium shoot contained water-soluble materials that were toxic to root growth of velvetleaf and wheat. At 4%

  11. PERFORMANCE MEASUREMENT TOOLS FOR HIGH-LEVEL PARALLEL PROGRAMMING LANGUAGES

    E-print Network

    Miller, Barton P.

    Kunchithapadam, Mark Cal- laghan, Karen Karavanic, Tia Newhall, Ari Tamches, Christopher Maguire, Mike McMenemy, Joerg Micheel, Chi-Ting Lam, Marcelo Goncalves, Oscar Naim, Mark McAuliffe, and Suhui #12;ii Chiang. Special thanks to Jeff and Mark for distinguished service as officemates. My friends have been

  12. PERFORMANCE MEASUREMENT TOOLS FOR HIGH LEVEL PARALLEL PROGRAMMING LANGUAGES

    E-print Network

    Miller, Barton P.

    Kunchithapadam, Mark Cal­ laghan, Karen Karavanic, Tia Newhall, Ari Tamches, Christopher Maguire, Mike McMenemy, Joerg Micheel, Chi­Ting Lam, Marcelo Goncalves, Oscar Naim, Mark McAuliffe, and Suhui #12; ii Chiang. Special thanks to Jeff and Mark for distinguished service as officemates. My friends have been

  13. A NEW CROSS-DIAMOND SEARCH ALGORITHM FOR FAST BLOCK MATCHING MOTION ESTIMATION

    E-print Network

    Po, Lai-Man

    A NEW CROSS-DIAMOND SEARCH ALGORITHM FOR FAST BLOCK MATCHING MOTION ESTIMATION Chi-Wai Lam 1 , Lai-center-biased characteristic of the real world video sequences, an improved version of the well- known cross diamond search results show that this new cross-diamond search algorithm could achieve much higher computational

  14. Millimeter-Wave Spectroscopy of Aminomalononitrile

    NASA Astrophysics Data System (ADS)

    Motiyenko, Roman A.; Margules, Laurent; Guillemin, Jean-Claude

    2013-06-01

    In low-temperature conditions of the interstellar medium pure HCN and mixtures of HCN with H_2O and NH_3 subjected to high-energy sources can be converted into different HCN polymers. In this context the HCN trimer -- aminomalononitrile (AMN, H_2NCH(CN)_2) is an interesting candidate for astrophysical detection. We measured the rotational spectrum of AMN using the Lille BWO-based fast scan spectrometer (120 -- 180 GHz) and the spectrometer based on solid state sources (225 -- 250 GHz). The spectroscopic work was supported by high level ab initio calculations. The spectra observed were assigned only to the asymmetric conformer. The first analysis revealed the regular doublet splittings of rotational lines indicating the possibility of large-amplitude motion (LAM) of amino group. Further examination of the spectra revealed the existence of additional splittings most probably due to the second LAM of amino group. Since the equilibrium structure of AMN has no symmetry, in the group theoretical formalism these two LAMs can be described using G_4 permutation-inversion group. Despite the complexity of the LAMs all the assigned rotational transitions were fitted within experimental accuracy using Pickett's SPFIT program. As a result, a reliable dataset for astrophysical observations was provided. This work is supported by the french program "Physique et Chimie du Milieu Interstellaire (PCMI, INSU-CNRS)" and the Centre National d'Etudes Spatiales (CNES). Gerakines, P.A., Moore, M.H, Hudson, R.L., Icarus, 2004, 170, 203

  15. Potential mechanisms of hypocholesterolaemic effect of Thai spices\\/dietary extracts

    Microsoft Academic Search

    Acharaporn Duangjai; Kornkanok Ingkaninan; Nanteetip Limpeanchob

    2011-01-01

    Several Thai spices\\/dietary ingredients were previously shown to have hypocholesterolaemic effects. These studies were mostly conducted in animal models in which the mechanisms of action were not yet well-established. Therefore, this study aimed to investigate the potential mechanism of hypocholesterolaemic action of 12 selected plants, namely Hibiscus sabdariffa L., Moringa oleifera Lam., Cucurbita moschata Duchesne, Ananas comosus (L.) Merr., Zingiber

  16. JOURNAL OF NEIJROPHYSIOLOGY RAPID PUBLICATIONVol. 76, No. 3, September 1996. Pridrd in U.S.A. Neuronal Synchronization of Tonically Active Neurons in the Striatum

    E-print Network

    Friedman, Nir

    do fire synchronously, and whether dopamine depletion affects their syn- chronization, we recorded of TANS (33 of 54; 61.1%) fire synchronously at -+60-ms delay. Correlated activity was more common between striatal compartments (Bo- lam and Bennet 1995). The pathological hallmark of Parkin- son's disease

  17. Behavior of Enterobacter amnigenus and Salmonella typhimurium in Crottin goat's cheese: Influence of fluctuating storage temperature

    Microsoft Academic Search

    L. M. Tamagnini; G. B. de Sousa; R. D. González; C. E. Budde

    2008-01-01

    The fully integrated cold chain is an important part in the implementation of Hazard Analysis and Critical Control Points procedures by food industry. The aim of this work was to determine the behavior of Enterobacter amnigenus (ATCC 33072 and an isolated cheese strain: LAM 200) and Salmonella typhimurium in Crottin goat's cheese during chill storage and when the cold chain

  18. The use of water lettuce (Pistia stratiotes L.) for rhizofiltration of a highly polluted solution by cadmium and lead

    Microsoft Academic Search

    TomᚠVeselý; Pavel Tlustoš; Ji?ina Száková

    2011-01-01

    The effectiveness of heavy metal uptake from contaminated nutrient solution by four aquatic macrophytes (Pistia stratiotes L., Salvinia auriculata Aubl., Salvinia minima Baker, and Azolla filiculoides Lam) was estimated in this study. The influence of cadmium (3.5 mg L and 10.5 mg L) and lead (25 mg L and 125 mg L) on the stress symptoms was observed through the

  19. Lee, et al., "Wafer Nanotopography Effects..." MRS Spring Meeting April 20011 Brian Lee, Duane S. Boning (MIT)

    E-print Network

    Boning, Duane S.

    Lee, et al., "Wafer Nanotopography Effects..." MRS Spring Meeting ­ April 20011 Brian Lee, Duane SJiang Sun (Philips Semiconductor) Michael Lacy (Lam Research) #12;Lee, et al., "Wafer Nanotopography Effects..." MRS Spring Meeting ­ April 20012 "Nanotopography" refers to wafer surface variations with: 1. Lateral

  20. Learning Bayesian Belief Networks An approach based on the MDL Principle

    E-print Network

    Bacchus, Fahiem

    is theoretically founded on Rissanen's Minimum Description Length (MDL) Principle 13]. It is well known is based on Rissanen's Minimal Description Length (MDL) principle, which is partic- ularly well suitedLearning Bayesian Belief Networks An approach based on the MDL Principle Wai Lam and Fahiem Bacchus