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1

Assessment of Antioxidant Potential, Total Phenolics and Flavonoids of Different Solvent Fractions of Monotheca Buxifolia Fruit  

PubMed Central

Objectives This study was conducted to investigate the antioxidant potential of methanol extract and its derived fractions (hexane, ethyl acetate, butanol, and aqueous) of fruits of Monotheca buxifolia (Falc.) Dc., a locally used fruit in Pakistan. Methods Dried powder of the fruit of M. buxifolia was extracted with methanol and the resultant was fractionated with solvents having escalating polarity; n-hexane, chloroform, ethyl acetate, n-butanol and the residual soluble aqueous fraction. Total phenolic and total flavonoid contents were estimated for the methanol and various fractions. These fractions were also subjected to various in vitro assays to estimate the scavenging activity for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide, hydroxyl, hydrogen peroxide and reductive ability for ferric ions and phosphomolybdate assay. Results The n-butanol, aqueous and methanol fractions possessed high amount of phenolics and flavonoids compared with other fractions, and subsequently showed a pronounced scavenging activity on DPPH, ABTS, superoxide, hydroxyl and hydrogen peroxide radicals and had a potent reductive ability on ferric ion and phosphomolybdate assay. There was a found significant correlation between total phenolic and flavonoid contents and EC50 of DPPH, superoxide, hydrogen peroxide radical and phosphomolybdate assays, whereas a nonsignificant correlation was found with the hydroxyl radical and ABTS radical assay. Conclusion M. buxifolia fruit can be used as natural antioxidant source to prevent damage associated with free radicals. PMID:24298440

Jan, Shumaila; Khan, Muhammad Rashid; Rashid, Umbreen; Bokhari, Jasia

2013-01-01

2

How Is LAM Treated?  

MedlinePLUS

... helps stabilize lung function, reduce symptoms, and improve quality of life for people who have LAM. Sirolimus does have ... Transplant Lung transplants can improve lung function and quality of life in patients who have advanced LAM. However, lung ...

3

Phytochemistry and Pharmacology of Berberis Species  

PubMed Central

The genus Berberis (Berberidaceae) includes about 500 species worldwide, some of which are widely cultivated in the north-eastern regions of Iran. This genus consists of spiny deciduous evergreen shrubs, characterized by yellow wood and flowers. The cultivation of seedless barberry in South Khorasan goes back to two hundred years ago. Medicinal properties for all parts of these plants have been reported, including: Antimicrobial, antiemetic, antipyretic, antioxidant, anti-inflammatory, anti-arrhythmic, sedative, anti-cholinergic, cholagogic, anti-leishmaniasis, and anti-malaria. The main compounds found in various species of Berberis, are berberine and berbamine. Phytochemical analysis of various species of this genus revealed the presence of alkaloids, tannins, phenolic compounds, sterols and triterpenes. Although there are some review articles on Berberis vulgaris (as the most applied species), there is no review on the phytochemical and pharmacological activities of other well-known species of the genus Berberis. For this reason, the present review mainly focused on the diverse secondary metabolites of various species of this genus and the considerable pharmacological and biological activities together with a concise story of the botany and cultivation. PMID:24600191

Mokhber-Dezfuli, Najmeh; Saeidnia, Soodabeh; Gohari, Ahmad Reza; Kurepaz-Mahmoodabadi, Mahdieh

2014-01-01

4

LAM/MPI  

NSDL National Science Digital Library

LAM/MPI is a high quality implementation of the Message Passing Interface (MPI) Standard. LAM/MPI provides high performance on a variety of platforms, from small off-the-shelf single CPU clusters to large SMP machines with high speed networks, even in heterogenous environments. In addition to high performance, LAM provides a number of usability features key to developing large scale MPI applications.

Llamas, The

5

LAMS IN TESOL: SKETCHING POTENTIAL  

Microsoft Academic Search

This paper 1 aims to provide some initial ideas on how LAMS might be utilised in TESOL 2 . Even though this research area requires significant development, it is held that there is growing potential and justification to use LAMS in TESOL. To this end an attempt will be made to illustrate how LAMS, with its increasing number of authoring

Chris Alexander

6

The Invasion Ecology of Japanese Barberry (Berberis thunbergii) in the New England Landscape  

Microsoft Academic Search

Japanese barberry (Berberis thunbergii) has been characterized as one of the most widely known and planted exotic shrubs in the United States. It was first introduced to the US in the late 1800s. By 1920 the planting of Japanese barberry was encouraged as an ornamental shrub replacing the common barberry (Berberis vulgaris). Japanese barberry began spreading from cultivation in suburban

John A. Silander; Debra M. Klepeis

1999-01-01

7

The nucleolus Yun Wah Lam*, Laura Trinkle-  

E-print Network

The nucleolus Yun Wah Lam*, Laura Trinkle- Mulcahy*, and Angus I. Lamond Wellcome Trust Biocentre and stably expressed in HeLa cells (Trinkle- Mulcahy et al., 2003). Like all other intranuclear structures Lam, Laura Trinkle-Mulcahy and Angus I. Lamond jcs.biologists.org Nucleolus Transmission electron

Trinkle-Mulcahy, Laura

8

Influence of drying conditions on the effective moisture diffusivity, energy of activation and energy consumption during the thin-layer drying of berberis fruit (Berberidaceae)  

Microsoft Academic Search

Berberis is known as a medicinal and ornamental plant in the world. Berberis fruit is used in medicine to cure liver, neck and stomach cancer, blood purification and mouth scent. Dried berberis fruit using new technology was preserved for relatively long time. Thin-layer drying simulation was used to obtain experiment data, using laboratory scale hot-air dryer of the static tray.

Mortaza Aghbashlo; Mohammad H. kianmehr; Hadi Samimi-Akhijahani

2008-01-01

9

Wilbur A. Lam Title: Assistant Professor  

E-print Network

Institute of Technology and our affiliations with the Children's Healthcare of Atlanta hospitals. #12;AwardsWilbur A. Lam Title: Assistant Professor University Affiliations Georgia Institute of Technology. Coulter Dept. of Biomedical Engineering Technology Areas Biomedical Materials & Devices MEMS

Garmestani, Hamid

10

Ascomycetes on Butea monosperma (Lam) Kuntze  

Microsoft Academic Search

In this paper Herpotrichia monospermae, sp. nov, Pleospora chandensis sp. nov, Julella kanargaoensis sp. nov. are described as new species and a new host record for an ascomyceteus fungus on Butea monosperma (LAM) KUNTZE. Further studies revealed the occurrance of the three Ascomycetes on the same host. These are described here as new to science on the basis of host

P. G. Sathe; K. M. Mogarkar

1973-01-01

11

C.20 Local Rings T. Y. Lam  

E-print Network

C.20 Local Rings T. Y. Lam. A commutative ring R is called a local ring if it has exactly one maximal id* *eal, or equivalently, R 6= 0 and the nonunits of R form an ideal m. For such a local ri* *ng (R, m), R=m is a field, called the residue field

Lam, Tsit-Yuen

12

Berberis aetnensis C. Presl. extracts: antimicrobial properties and interaction with ciprofloxacin.  

PubMed

Previous research showed that berberine-containing Berberis species synthesise the substances 5'-methoxyhydnocarpin-D (5'-MHC-D) and pheophorbide a, which have no antimicrobial activity but inhibit the expression of multidrug resistant efflux pumps (MDRs) in Staphylococcus aureus and potentiate the action of berberine. The MDR pumps extrude synthetic and natural antimicrobials from bacterial cells. We searched for these compounds in Berberis aetnensis C. Presl. (Berberidaceae), an endemic plant of the volcano Mount Etna. This work confirms the presence of pheophorbide a and permits us to hypothesise the presence of 5'-MHC-D in leaf extracts. In fact, the activity of ciprofloxacin was improved when two chromatographic fractions isolated from leaf extracts were added. These results are indicative of the presence of MDR pump inhibitors. Moreover, crude extracts were tested on several micro-organisms and showed antimicrobial activity mainly against Gram-positive bacteria and yeasts. PMID:12842327

Musumeci, Rosario; Speciale, A; Costanzo, R; Annino, A; Ragusa, S; Rapisarda, A; Pappalardo, M S; Iauk, L

2003-07-01

13

Inhibition and dissolution of calcium oxalate crystals by Berberis Vulgaris-Q and other metabolites  

NASA Astrophysics Data System (ADS)

Inhibition and dissolution of calcium oxalate crystals in solutions containing a homoeopathic medicine Berberis Vulgaris-Q, amino acids such as aspartic acid, glutamic acid, ?-keto glutaric acid, a naturally occurring inhibitor and juices of some fruits of citrus group such as lemon, orange and mausammi have been studied. Considerable solubility was observed and found that in each case solubility changes with time or concentration periodically. Chelation may be one of the reasons for inhibition.

Das, Ishwar; Gupta, S. K.; Pandey, V. N.; Ansari, Shoeb A.

2004-07-01

14

"Getting stuck with LAM": patients perspectives on living with Lymphangioleiomyomatosis  

PubMed Central

Background Lymphangioleiomyomatosis (LAM) is a rare, progressive lung disease that affects almost exclusively women and is most often diagnosed before menopause. The main symptom of LAM is shortness of breath. LAM patients’ perceptions of how the disease impacts their lives is largely unknown, but such information could be useful to generate patient reported outcome measures for use in drug trials (or other research studies) and to formulate interventions aimed at easing the burdens LAM imposes on patients. Objective To capture patients’ perceptions of how LAM affects their lives. Methods We used reflexive team analysis to analyze transcripts from semi-structured focus groups conducted with LAM patients at LAMposium 2013. We sought to determine what patients perceive as the primary symptoms of LAM and how the disease affects them in their daily lives. Results The 37 participants described seven primary symptoms of LAM and five common psychological experiences from living with the disease. Shortness of breath and low energy (or fatigue) dominated the symptomatic picture; cough, sensations in the chest, difficulty sleeping, gastrointestinal issues, and mild cognitive difficulties were less common. The common psychological experiences participants reported included frustration, worry, loss of identity, embarrassment, and in some participants, a healthy defiance against the disease. Conclusions Patients perceive the physical symptoms from LAM to be intrusive and limiting. Women living with LAM are frustrated by their physical limitations, and they worry about what the future will be like if the disease progresses. Therapeutic interventions should take aim at improving these perceptions. PMID:24885591

2014-01-01

15

Universal Plant DNA Barcode Loci May Not Work in Complex Groups: A Case Study with Indian Berberis Species  

PubMed Central

Background The concept of DNA barcoding for species identification has gained considerable momentum in animals because of fairly successful species identification using cytochrome oxidase I (COI). In plants, matK and rbcL have been proposed as standard barcodes. However, barcoding in complex genera is a challenging task. Methodology and Principal Findings We investigated the species discriminatory power of four reportedly most promising plant DNA barcoding loci (one from nuclear genome- ITS, and three from plastid genome- trnH-psbA, rbcL and matK) in species of Indian Berberis L. (Berberidaceae) and two other genera, Ficus L. (Moraceae) and Gossypium L. (Malvaceae). Berberis species were delineated using morphological characters. These characters resulted in a well resolved species tree. Applying both nucleotide distance and nucleotide character-based approaches, we found that none of the loci, either singly or in combinations, could discriminate the species of Berberis. ITS resolved all the tested species of Ficus and Gossypium and trnH-psbA resolved 82% of the tested species in Ficus. The highly regarded matK and rbcL could not resolve all the species. Finally, we employed amplified fragment length polymorphism test in species of Berberis to determine their relationships. Using ten primer pair combinations in AFLP, the data demonstrated incomplete species resolution. Further, AFLP analysis showed that there was a tendency of the Berberis accessions to cluster according to their geographic origin rather than species affiliation. Conclusions/Significance We reconfirm the earlier reports that the concept of universal barcode in plants may not work in a number of genera. Our results also suggest that the matK and rbcL, recommended as universal barcode loci for plants, may not work in all the genera of land plants. Morphological, geographical and molecular data analyses of Indian species of Berberis suggest probable reticulate evolution and thus barcode markers may not work in this case. PMID:21060687

Roy, Sribash; Tyagi, Antariksh; Shukla, Virendra; Kumar, Anil; Singh, Uma M.; Chaudhary, Lal Babu; Datt, Bhaskar; Bag, Sumit K.; Singh, Pradhyumna K.; Nair, Narayanan K.; Husain, Tariq; Tuli, Rakesh

2010-01-01

16

The Role of Laparoscopic-Assisted Myomectomy (LAM)  

PubMed Central

Laparoscopic myomectomy has recently gained wide acceptance. However, this procedure remains technically highly demanding and concerns have been raised regarding the prolonged time of anesthesia, increased blood loss, and possibly a higher risk of postoperative adhesion formation. Laparoscopic-assisted myomectomy (LAM) is advocated as a technique that may lessen these concerns regarding laparoscopic myomectomy while retaining the benefits of laparoscopic surgery, namely, short hospital stay, lower costs, and rapid recovery. By decreasing the technical demands, and thereby the operative time, LAM may be more widely offered to patients. In carefully selected cases, LAM is a safe and efficient alternative to both laparoscopic myomectomy and myomectomy by laparotomy. These cases include patients with numerous large or deep intramural myomas. LAM allows easier repair of the uterus and rapid morcellation of the myomas. In women who desire a future pregnancy, LAM may be a better approach because it allows meticulous suturing of the uterine defect in layers and thereby eliminates excessive electrocoagulation. PMID:11719974

Nezhat, Ceana H.; Nezhat, Farr.; Nezhat, Camran

2001-01-01

17

An antitumor promoter from Moringa oleifera Lam  

Microsoft Academic Search

In the course of studies on the isolation of bioactive compounds from Philippine plants, the seeds of Moringa oleifera Lam. were examined and from the ethanol extract were isolated the new O-ethyl-4-(?-l-rhamnosyloxy)benzyl carbamate (1) together with seven known compounds, 4(?-l-rhamnosyloxy)-benzyl isothiocyanate (2), niazimicin (3), niazirin (4), ?-sitosterol (5), glycerol-1-(9-octadecanoate) (6), 3-O-(6?-O-oleoyl-?-d-glucopyranosyl)-?-sitosterol (7), and ?-sitosterol-3-O-?-d-glucopyranoside (8). Four of the isolates (2,

Amelia P. Guevara; Carolyn Vargas; Hiromu Sakurai; Yasuhiro Fujiwara; Keiji Hashimoto; Takashi Maoka; Mutzuo Kozuka; Yoshohiro Ito; Harukuni Tokuda; Hoyoku Nishino

1999-01-01

18

Structure and Dynamics of Populations of Japanese Barberry (Berberis Thunbergii DC.) in Deciduous Forests of New Jersey  

Microsoft Academic Search

Japanese barberry, Berberis thunbergii DC., has become a prominent exotic species in deciduous forests throughout the eastern and midwestern US. Populations range from small plants occurring at low densities to dense, impenetrable thickets of plants with up to 40 stems\\/individual. A study was undertaken at Morristown National Historical Park in New Jersey to document plant densities, plant size, recruitment through

Joan G. Ehrenfeld

1999-01-01

19

Aqueous extract of Berberis integerrima root improves renal dysfunction in streptozotocin induced diabetic rats  

PubMed Central

Objective: Barberry root extract contains various alkaloids that are considered as antioxidants. Beneficial effect of aqueous extract of Berberis integerrima root (AEBIR) was evaluated for renal function in diabetic rats induced by STZ. Material and Methods: Diabetes was induced by i.p. injection of streptozotocin (65 mg/kg bw) to rats, after 15 h of fasting. Diabetic rats were randomly grouped and treated daily with AEBIR and glibenclamide by gavage for 42 days. After 6 weeks of study, all the rats were sacrificed and some biochemical parameters of serum and urine were measured and their kidneys tissues were processed for light microscopy. Results: Streptozotocin induced a significant rise in fasting blood glucose, serum creatinine, blood urea nitrogen, urine glucose, urine protein, urine albumin, and water intake and a significant decrease in body weight, serum protein, urine urea, and urine creatinine. There was a significant restoration of these parameters to near normal after administration of the AEBIR and also by glibenclamide (0.6 mg/kg bw). The activity of the extract at dose of 500 mg/kg in all parameters except blood glucose and urine glucose was more than that of the standard drug, glibenclamide (0.6 mg/kg, p.o.). Histopathological changes of kidney samples were comparable with respective control. Conclusion: These results suggested that aqueous extract of Berberis Integerrima root improves renal dysfunction in streptozotocin-induced diabetic rats through controlling blood glucose and renal protective effects. PMID:25050261

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid; Ilkhanipoor, Minoo

2013-01-01

20

Comparison of the antidiabetic activity of Berberis lyceum root extract and berberine in alloxan-induced diabetic rats.  

PubMed

Berberine has been shown to have hypoglycaemic activity in several in vitro and in vivo models, although the mechanism of action is not fully known. Berberis lyceum Royle root produces high concentrations of berberine, and in traditional medicine, the whole extract of this plant is used widely to treat diabetes. The antidiabetic activity of the ethanol root extract of Berberis lyceum was compared with pure berberine in normal and alloxan-diabetic rats using similar doses of each. The concentration of berberine in the extract was determined to be 80% dry weight with only trace amounts of other alkaloids present. The purpose of the study was to investigate the effects of berberine and a whole extract of Berberis lyceum on blood glucose and other parameters associated with diabetes, to compare the effects of the crude extract with those of pure berberine and thus validate its use as a therapeutic agent, and finally to identify any contribution of the other components of the extract to these effects. Oral administration of 50 mg/kg of Berberis extract and berberine to normal and experimental diabetic rats produced a significant (p < 0.05) reduction in blood glucose levels from days 3-7 days of treatment. Significant effects were also observed on the glucose tolerance, glycosylated haemoglobin, serum lipid profiles and body weight of experimental animals. Berberis extract and berberine demonstrated similar effects on all parameters measured, and although the extract was comparable in efficacy to berberine, it did not produce any effects additional to those shown by pure berberine. The results support the use of the extract in traditional medicine, and demonstrate that apart from being a highly cost-effective means of treating with berberine, the total extract does not appear to confer any additional benefits or disadvantages compared with the pure compound. PMID:18729256

Gulfraz, M; Mehmood, S; Ahmad, A; Fatima, N; Praveen, Z; Williamson, E M

2008-09-01

21

UNDERSIDE FROM SOUTH BANKS; NOTICE NEW GLUE LAM CROSSBEAMS SISTERED ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

UNDERSIDE FROM SOUTH BANKS; NOTICE NEW GLUE LAM CROSSBEAMS SISTERED TO OLDER BEAMS, NEW STRINGERS AND COMPONENTS MAKE UP A NEARLY NEW SUPPORT SYSTEM - Short Bridge, Spanning South Santiam River at High Deck Road, Cascadia, Linn County, OR

22

UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kelsie Priest, Lahiru Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam  

E-print Network

Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam UBC Botanical Garden Redevelopment of a project/report". #12;UBC Botanical Garden Redevelopment Detailed Design Report Prepared for UBC CIVIL 446 and UBC Botanical Garden Prepared by CIVIL 446 - Group One Mike Kai Hin Lam # Kelsie Priest # Lahiru

23

Effects of Aqueous Extract of Berberis integerrima Root on Some Physiological Parameters in Streptozotocin-Induced Diabetic Rats  

PubMed Central

Diabetes mellitus is a common endocrine disorder. Anti-diabetic agents from natural and synthetic sources are available for the treatment of this disease. Berberis integerrima is a medicinal shrub used in conventional therapy for a number of diseases. The aim of the present study was to investigate the effects of aqueous extract of Berberis integerrima root (AEBI) on some physiological parameters in normal and streptozotocin-induced (STZ-induced) diabetic male Wistar rats. STZ-induced diabetic rats showed significant increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C), creatinine (Cr), urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin while body weight, high density lipoprotein HDL-cholesterol (HDL-C) and total protein levels were significantly decreased compared to normal rats. Treatment of diabetic rats with different doses of aqueous extract of Berberis integerrima root (250 and 500 mg/Kg bw) resulted in a significant decrease in blood glucose, triglycerides, cholesterol, LDL-cholesterol, ALT, AST, ALP, total bilirubin, creatinine and urea while HDL-cholesterol and total protein levels were markedly increased after six weeks compared to untreated diabetic rats. The effects of the AEBI at dose of 500 mg/Kg in all parameters except blood glucose (similar) is more than to the standard drug, glibenclamide (0.6 mg/Kg, p.o.). The results of this study indicate that the tested aqueous extract of Berberis integerrima root possesses hypoglycemic, hypolipidemic and antioxidant effects in STZ-induced diabetic rats. PMID:24250618

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid; Ilkhanipoor, Minoo

2013-01-01

24

An antitumor promoter from Moringa oleifera Lam.  

PubMed

In the course of studies on the isolation of bioactive compounds from Philippine plants, the seeds of Moringa oleifera Lam. were examined and from the ethanol extract were isolated the new O-ethyl-4-(alpha-L-rhamnosyloxy)benzyl carbamate (1) together with seven known compounds, 4(alpha-L-rhamnosyloxy)-benzyl isothiocyanate (2), niazimicin (3), niazirin (4), beta-sitosterol (5), glycerol-1-(9-octadecanoate) (6), 3-O-(6'-O-oleoyl-beta-D-glucopyranosyl)-beta-sitosterol (7), and beta-sitosterol-3-O-beta-D-glucopyranoside (8). Four of the isolates (2, 3, 7, and 8), which were obtained in relatively good yields, were tested for their potential antitumor promoting activity using an in vitro assay which tested their inhibitory effects on Epstein-Barr virus-early antigen (EBV-EA) activation in Raji cells induced by the tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA). All the tested compounds showed inhibitory activity against EBV-EA activation, with compounds 2, 3 and 8 having shown very significant activities. Based on the in vitro results, niazimicin (3) was further subjected to in vivo test and found to have potent antitumor promoting activity in the two-stage carcinogenesis in mouse skin using 7,12-dimethylbenz(a)anthracene (DMBA) as initiator and TPA as tumor promoter. From these results, niazimicin (3) is proposed to be a potent chemo-preventive agent in chemical carcinogenesis. PMID:10209341

Guevara, A P; Vargas, C; Sakurai, H; Fujiwara, Y; Hashimoto, K; Maoka, T; Kozuka, M; Ito, Y; Tokuda, H; Nishino, H

1999-04-01

25

Using New Data to Re ne a Bayesian Network Wai Lam and Fahiem Bacchus  

E-print Network

Using New Data to Re ne a Bayesian Network Wai Lam and Fahiem Bacchus Department of Computer be usefully employed for reasoning about its domain. However, in the period that the network Wai Lam's work

Bacchus, Fahiem

26

Spine production is induced by fire: a natural experiment with three Berberis species  

NASA Astrophysics Data System (ADS)

Earlier studies indicate that some plant species allocate more mass to produce longer spines in shoots resprouting after browsing. Here we present, for the first time, evidence that fire induces a similar response. Many terrestrial herbivores may benefit from fire through the enhanced availability of fast growing species colonizing or re-sprouting in burned areas. It is less clear whether post-fire plant growth responds to the enhanced risk of herbivory by an increased investment in defensive traits. In this study, we tested whether the production of spines is influenced by the set of environmental conditions that result from fire events. We compared the resource allocation pattern of resprouting shoots from three Berberis species growing in two areas that burned 1999 with samples collected from unburned areas within the same plant communities. We divided the shoot into three main components: supporting tissue (twigs), assimilating tissue (leaves) and defensive structures (spines). We found that plants resprouting after fire allocated more mass to spines and leaves but not twigs. This resulted in a higher density of both spines and leaves. Spines were significantly longer in plants resprouting after fire. Leaves were shorter at the apical end of the shoot, but did not show any significant change in size following fire. We suggest that this type of post-fire response may be a general adaptation to pruning and leaf picking by browsing herbivores in arid and semi-arid regions. Changes in the browsing pressure following fire will determine the fitness value of this response.

Gowda, Juan; Raffaele, Estela

2004-12-01

27

Polyphenols and antioxidant activity of calafate ( Berberis microphylla ) fruits and other native berries from Southern Chile.  

PubMed

Calafate ( Berberis microphylla ) is a native berry grown in the Patagonian area of Chile and Argentina. In the present study the phenolic composition and antioxidant activity of its fruits were studied and also compared with data obtained for other berry fruits from southern Chile including maqui ( Aristotelia chilensis ) and murtilla ( Ugni molinae ). Polyphenolic compounds in calafate fruit were essentially present in glycosylated form, 3-glucoside conjugates being the most abundant anthocyanins. The anthocyanin content in calafate berries (17.81 +/- 0.98 micromol g(-1)) and flavonol level (0.16 +/- 0.01 micromol g(-1)) are comparable with those found in maqui (17.88 +/- 1.15 and 0.12 +/- 0.01 micromol g(-1), respectively); however, maqui shows lower flavan-3-ol concentration than calafate (0.11 +/- 0.01 and 0.24 +/- 0.03 micromol g(-1), respectively). Maqui and calafate show high antioxidant activity, which correlates highly with total polyphenol content and with anthocyanin concentration. PMID:20438111

Ruiz, Antonieta; Hermosín-Gutiérrez, Isidro; Mardones, Claudia; Vergara, Carola; Herlitz, Erika; Vega, Mario; Dorau, Carolin; Winterhalter, Peter; von Baer, Dietrich

2010-05-26

28

Preliminary study about the possible glycemic clinical advantage in using a fixed combination of Berberis aristata and Silybum marianum standardized extracts versus only Berberis aristata in patients with type 2 diabetes  

PubMed Central

Background Berberine is an isoquinoline alkaloid widely used to improve the glucidic and lipidic profiles of patients with hypercholesterolemia, metabolic syndrome, and type 2 diabetes. The limitation of berberine seems to be its poor oral bioavailability, which is affected by the presence, in enterocytes, of P-glycoprotein – an active adenosine triphosphate (ATP)-consuming efflux protein that extrudes berberine into the intestinal lumen, thus limiting its absorption. According to some authors, silymarin, derived from Silybum marianum, could be considered a P-glycoprotein antagonist. Aim The study aimed to evaluate the role played by a possible P-glycoprotein antagonist (silymarin), when added to a product containing Berberis aristata extract, in terms of benefits to patients with type 2 diabetes. Methods The study enrolled 69 patients with type 2 diabetes in suboptimal glycemic control who were treated with diet, hypoglycemic drugs, and in cases of concomitant alterations of the lipid profile, hypolipidemic agents. The patients received an add-on therapy consisting of either a standardized extract of Berberis aristata (titrated in 85% berberine) corresponding to 1,000 mg/day of berberine, or Berberol®, a fixed combination containing the same standardized extract of Berberis aristata plus a standardized extract of Silybum marianum (titrated as >60% in silymarin), for a total intake of 1,000 mg/day of berberine and 210 mg/day of silymarin. Results Both treatments similarly improved fasting glucose, total cholesterol, low-density lipoprotein (LDL) cholesterol, triglyceride, and liver enzyme levels, whereas glycosylated hemoglobin (HbA1c) values were reduced to a greater extent by the fixed combination. Conclusion The association of berberine and silymarin demonstrated to be more effective than berberine alone in reducing HbA1c, when administered at the same dose and in the form of standardized extracts in type 2 diabetic patients. PMID:24277991

Di Pierro, Francesco; Putignano, Pietro; Villanova, Nicola; Montesi, Luca; Moscatiello, Simona; Marchesini, Giulio

2013-01-01

29

Sulphadimethoxine and Azolla filiculoides Lam.: a model for drug remediation  

Microsoft Academic Search

Plants can be an interesting tool for in situ remediation of drug contaminated waters. In a laboratory model Azolla filiculoides Lam., an aquatic fern known to absorb pollutants, has been exposed to an environmental persistent antibiotic commonly used in intensive farming, sulphadimethoxine (S), to test its bioremediation capability. In a 5 week experiment, plants were cultivated outdoor at four drug

Cinzia Forni; Antonella Cascone; Maurizio Fiori; Luciana Migliore

2002-01-01

30

Antimicrobial action of the leaf extract of moringa oleifera lam.  

PubMed

The ethnolic extract of the leaves of Moringa oleifera Lam. (Fam. Moringaceae) was tested for antimicrobial activities against Gram Positive - Bacillus cereus, Bacillus subtilis Staphylococcus aureus, Sarcina lutea: Gram negative - Escherichia coli and Acid fast Mycobacterium phlei. Significant antimicrobial activity of the extract was found in this study. PMID:22556699

Pal, S K; Mukherjee, P K; Saha, K; Pal, M; Saha, B P

1995-01-01

31

Some pharmacognostical characteristics of moringa oleifera lam. Leaves.  

PubMed

The macroscopic character of the leaflets ash values extractive values by different solvent extractive behavior on treatment with different chemical reagents fluorescence characters by treatment with different chemical reagents of the powdered leaves of Maoringa oleifera lam (fam moringaceae) were studied to fix some pharmacognostical parameters, chemical group test of different extracts of the leaves were also performed. PMID:22556759

Pal, S K; Mukherjee, P K; Saha, K; Pal, M; Saha, B P

1996-04-01

32

Combinatorial approach to Mathieu and Lam\\'e equations  

E-print Network

Based on some recent progress on a relation between four dimensional super Yang-Mills gauge theory and quantum integrable system, we give a combinatorial method to derive the asymptotic expansions for eigenvalues of the Mathieu and Lam\\'{e} equations. The WKB method is also used to extend the expansions in one asymptotic region to other asymptotic regions.

He, Wei

2011-01-01

33

Cytotoxic evaluation of essential oil from Zanthoxylum rhoifolium Lam. leaves  

Microsoft Academic Search

Zanthoxylum rhoifolium Lam is a plant popularly used as antimicrobial, for malaria and inflammatory treatment. The essential oil of Z. rhoifolium was extracted and its cytotoxic effects against HeLa (human cervical carcinoma), A-549 (human lung carcinoma), HT-29 (human colon adenocarcinoma), Vero (monkey kidney) cell lines and mice macrophages were evaluated. Some of the terpenes of its essential oil (?-caryophyllene, ?-humulene,

Saulo Luis da Silva; Patrícia Maria Figueiredo; Tomomasa Yano

2007-01-01

34

Sexual structures and recombination of the wheat rust fungus Puccinia striiformis on Berberis vulgaris.  

PubMed

An isolate of the basidiomycete Puccinia striiformis, which causes yellow (stripe) rust on wheat, was selfed on the newly discovered alternate host, Berberis vulgaris. This allowed a study of the segregation of molecular markers and virulence in the progeny isolates, and of the development of fungal sexual structures and spore forms. Pycnia and aecia were obtained after inoculation of B. vulgaris with basidiospores resulting from germinating teliospores from infected wheat leaves. Subsequent inoculation of wheat with aeciospores from bulked aecia resulted in 16 progeny isolates of the S1 generation. Genotyping with 42 simple sequence repeat (SSR) markers confirmed a parental origin of progeny isolates. Of the 42 analyzed loci, 15 were heterozygous in the parental isolate and 14 revealed segregation in the progenies. This resulted in 11 new multilocus genotypes (MLGs), which confirmed segregation following sexual reproduction. Additionally, parental and progeny isolates were phenotyped using a genetic stock of wheat genotypes representing 21 resistance genes. All S1 progeny isolates had virulence for 14 out of 15 loci where the parental isolate was virulent. This was consistent with the hypothesis that virulence in plant pathogens is often recessive to avirulence, i.e., only expressed in a homozygous state. Furthermore, no segregation was observed for five out of six loci, for which the parental isolate had an avirulent phenotype. The results for one of the two segregating virulence/avirulence loci suggested that the parental isolate was heterozygous with Avr alleles resulting in different but clearly avirulent phenotypes. The other locus indicated that additional genes modifying the phenotypic expression of avirulence were involved. PMID:25042987

Rodriguez-Algaba, Julian; Walter, Stephanie; Sørensen, Chris K; Hovmøller, Mogens S; Justesen, Annemarie F

2014-09-01

35

Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats  

PubMed Central

Objective To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris (B. vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins extract on groups of rats rendered diabetic by injection of streptozotocin. Methods The phytochemical tests to detect the presence of different compounds were based on the visual observation of color change or formation of precipitate after the addition of specific reagents. Diabetes was induced in rats by intraperitoneal (i.p.) injection of streptozotocin (STZ) at a dose of 65 mg/kg bw. The fasting blood glucose levels were estimated by glucose oxidase-peroxidase reactive strips (Dextrostix, Bayer Diagnostics). Blood samples were taken by cutting the tip of the tail. Serum cholesterol and serum triglycerides were estimated by enzymatic DHBS colorimetric method. Results Administration of 62.5 and 25.0 mg/kg of saponins and aqueous extract respectively in normal rats group shows a significant hypoglycemic activity (32.33% and 40.17% respectively) during the first week. However, diabetic group treated with saponin extract produced a maximum fall of 73.1% and 76.03% at day 1 and day 21 compared to the diabetics control. Also, blood glucose levels of the diabetic rats treated with aqueous extract showed decrease of 78.79% on the first day and the effect remains roughly constant during 3 week. Both extracts also declined significantly biochemical parameters (20.77%-49.00%). The control in the loss of body weight was observed in treated diabetic rats as compared to diabetic controls. Conclusions These results demonstrated significant antidiabetic effects and showed that serum cholesterol and serum triglycerides levels were decreased, significantly, consequently this plant might be of value in diabetes treatment. PMID:23569815

Meliani, Nawel; Dib, Mohamed El Amine; Allali, Hocine; Tabti, Boufeldja

2011-01-01

36

Berberis libanotica Ehrenb Extract Shows Anti-Neoplastic Effects on Prostate Cancer Stem/Progenitor Cells  

PubMed Central

Cancer stem cells (CSCs), including those of advanced prostate cancer, are a suggested reason for tumor resistance toward conventional tumor therapy. Therefore, new therapeutic agents are urgently needed for targeting CSCs. Despite the minimal understanding of their modes of action, natural products and herbal therapies have been commonly used in the prevention and treatment of many cancers. Berberis libanotica Ehrenb (BLE) is a plant rich in alkaloids which may possess anti-cancer activity and a high potential for eliminating CSCs. We tested the effect of BLE on prostate cancer cells and our data indicated that this extract induced significant reduction in cell viability and inhibited the proliferation of human prostate cancer cell lines (DU145, PC3 and 22Rv1) in a dose- and time-dependent manner. BLE extract induced a perturbation of the cell cycle, leading to a G0-G1 arrest. Furthermore, we noted 50% cell death, characterized by the production of high levels of reactive oxidative species (ROS). Inhibition of cellular migration and invasion was also achieved upon treatment with BLE extract, suggesting a role in inhibiting metastasis. Interestingly, BLE extract had a major effect on CSCs. Cells were grown in a 3D sphere-formation assay to enrich for a population of cancer stem/progenitor cells. Our results showed a significant reduction in sphere formation ability. Three rounds of treatment with BLE extract were sufficient to eradicate the self-renewal ability of highly resistant CSCs. In conclusion, our results suggest a high therapeutic potential of BLE extract in targeting prostate cancer and its CSCs. PMID:25380390

Eid, Assaad; Daoud, Georges; Hosry, Leina; Monzer, Alissar; Mouhieddine, Tarek H.; Hamade, Aline; Najjar, Fadia; Abou-Kheir, Wassim

2014-01-01

37

UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kelsie Priest, Lahiru Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam  

E-print Network

Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam UBC Botanical Garden Redevelopment CIVLGarden Redevelopment Prepared for UBC CIVIL 445 and UBC Botanical Garden Prepared by CIVIL 445 Group Botanical Garden Redevelopment" is submitted by Group 1 in response to request by UBC Botanical Garden

38

Hepatoprotective Effects of Berberis vulgaris L. Extract/? Cyclodextrin on Carbon Tetrachloride-Induced Acute Toxicity in Mice  

PubMed Central

The present study investigated the capacity of formulated Berberis vulgaris extract/?-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/?-cyclodextrin treatment prevents hepatic injury induced by CCl4 and can be considered for further nutraceutical studies. PMID:22942749

Hermenean, Anca; Popescu, Cristina; Ardelean, Aurel; Stan, Miruna; Hadaruga, Nicoleta; Mihali, Ciprian-Valentin; Costache, Marieta; Dinischiotu, Anca

2012-01-01

39

Century-old mystery of Puccinia striiformis life history solved with the identification of Berberis as an alternate host.  

PubMed

The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P. striiformis. Analyses using real-time polymerase chain reaction and DNA sequence confirmed the rust fungus as P. striiformis. Pycnia and aecia were produced on B. chinensis, B. holstii, B. koreana, and B. vulgaris after inoculation using germinating telia of P. striiformis f. sp. tritici. Wheat inoculated with aeciospores from B. chinensis resulted in uredinia, which demonstrated that Berberis spp. also serve as alternate hosts for the wheat stripe rust pathogen. The elucidation of the complete life history for P. striiformis f. sp. tritici will provide a powerful tool to rapidly advance our knowledge of the genetics of this rust fungus, and will lead to the development of improved strategies for a better control of stripe rust. PMID:20373963

Jin, Yue; Szabo, Les J; Carson, Martin

2010-05-01

40

Composition and Antimicrobial Activity of Salvia amplexicaulis Lam. Essential Oil  

Microsoft Academic Search

The chemical analysis of the Salvia amplexicaulis Lam. herb essential oil was performed using GC-FID and GC\\/MS. Fifty-one compounds (90.2% of the total oil) were identified. The oil was characterized by a high amount of sesquiterpenes (81.1%), with germacrene D (14.8%), viridiflorol (10.6%), caryophyllene oxide (10.5%), and ?-caryophyllene (9.4%) being the main components. The microbial growth inhibitory properties of the

Silvana Petrovi?; Milica Pavlovi?; Olga Tzakou; Maria Couladis; Marina Milenkovi?; Dragana Vu?i?evi?; Marjan Niketi?

2009-01-01

41

TSC2 epigenetic defect in primary LAM cells. Evidence of an anchorage-independent survival.  

PubMed

Tuberous sclerosis complex (TSC) is caused by mutations in TSC1 or TSC2 genes. Lymphangioleiomyomatosis (LAM) can be sporadic or associated with TSC and is characterized by widespread pulmonary proliferation of abnormal ?-smooth muscle (ASM)-like cells. We investigated the features of ASM cells isolated from chylous thorax of a patient affected by LAM associated with TSC, named LAM/TSC cells, bearing a germline TSC2 mutation and an epigenetic defect causing the absence of tuberin. Proliferation of LAM/TSC cells is epidermal growth factor (EGF)-dependent and blockade of EGF receptor causes cell death as we previously showed in cells lacking tuberin. LAM/TSC cells spontaneously detach probably for the inactivation of the focal adhesion kinase (FAK)/Akt/mTOR pathway and display the ability to survive independently from adhesion. Non-adherent LAM/TSC cells show an extremely low proliferation rate consistent with tumour stem-cell characteristics. Moreover, LAM/TSC cells bear characteristics of stemness and secrete high amount of interleukin (IL)-6 and IL-8. Anti-EGF receptor antibodies and rapamycin affect proliferation and viability of non-adherent cells. In conclusion, the understanding of LAM/TSC cell features is important in the assessment of cell invasiveness in LAM and TSC and should provide a useful model to test therapeutic approaches aimed at controlling their migratory ability. PMID:24606538

Lesma, Elena; Ancona, Silvia; Sirchia, Silvia M; Orpianesi, Emanuela; Grande, Vera; Colapietro, Patrizia; Chiaramonte, Eloisa; Di Giulio, Anna Maria; Gorio, Alfredo

2014-05-01

42

Time domain modelling of room acoustics Y.W. Lam and J. Hargreaves  

E-print Network

Time domain modelling of room acoustics Y.W. Lam and J. Hargreaves University of Salford, School of Computing, Science and Engineering, M5 4WT Salford, UK y.w.lam@salford.ac.uk Proceedings of the Acoustics advantages for room acoustics investigations. It directly generates the efficient for broadband calculations

Paris-Sud XI, Université de

43

Immunopathological stain of lipoarabinomannan-B (LAM-B) for diagnosis of leprosy.  

PubMed

We developed an immunopathological staining of LAM-B antigen in formalin-fixed paraffin-embedded tissues, and compared it with, PGL-I immunostaining, Fite Faraco's stain and periodic acid carbol pararosaniline (PACPR) stain. Out of the total 28 leprosy cases, 27 were positive to LAM-B immunostaining while 23 were positive to PGL-I stain. Fite's stain was positive in 21 cases while PACPR stain was positive in 24 cases. In scrofuloderma, LAM-B antigen was observed only in the granuloma while no other positive findings were noted with other stains. Normal skin did not give any positive findings with any of the stains. Other dermatoses showed no positive findings to any of the stains tested. LAM-B staining was observed in the nerve even in the absence of bacilli in leprosy tissues. Presence of LAM-B in the cutaneous nerves is helpful in discriminating leprosy from other mycobacterioses. Considering the high sensitivity of LAM-B and the predilection of M. leprae for the nerves, we concluded that LAM-B staining can be a useful new tool in the prompt diagnosis of leprosy, especially in suspected or early cases. PMID:7693642

Butt, K I; Kawatsu, K; Wang, T; Maeda, Y; Izumi, S

1993-03-01

44

Phytochemical Investigations on Chemical Constituents of Achillea tenuifolia Lam  

PubMed Central

Achillea tenuifolia Lam. (Asteraceae) afforded a methanolic extract from which after fractionation in solvents with different polarities, two known flavones 3’, 5- dihydroxy- 4’, 6, 7- trimethoxy flavone (eupatorine, compound 3), 5- hydroxy- 3’,4’, 6, 7- tetramethoxyflavone (compound 4), besides stearic acid (compound 1), lupeol (compound 2), daucosterol (?- sitosterol 3-O- ?- D- glucopyranoside, compound 5), 2, 4- dihydroxy methyl benzoate (compound 6) were isolated for the first time. The structure of isolated compounds was elucidated by means of different spectroscopic methods such as UV, IR, Mass and 1H- NMR (1D and 2D) and 13C-NMR. For further confirming the structures of isolated compounds, comparison of the spectral data of them with those reported in the litratures have been done.

Moradkhani, Shirin; Kobarfard, Farzad; Ayatollahi, Seyed Abdol Majid

2014-01-01

45

A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM).  

PubMed

Lymphangioleiomyomatosis (LAM) is a rare and progressive cystic lung condition affecting approximately 3.4-7.5/million women, with an average lag time between symptom onset and diagnosis of upwards of 4 years. The aim of this work was to identify altered proteins in LAM serum which may be potential biomarkers of disease. Serum from LAM patient volunteers and healthy control volunteers were pooled and analysis carried out using quantitative 4-plex iTRAQ technology. Differentially expressed proteins were validated using ELISAs and pathway analysis was carried out using Ingenuity Pathway Analysis. Fourteen proteins were differentially expressed in LAM serum compared to control serum (p<0.05). Further screening validated the observed differences in extracellular matrix remodelling proteins including fibronectin (30% decrease in LAM, p?=?0.03), von Willebrand Factor (40% reduction in LAM, p?=?0.03) and Kallikrein III (25% increase in LAM, p?=?0.03). Pathway networks elucidated the relationships between the ECM and cell trafficking in LAM. This study was the first to highlight an imbalance in networks important for remodelling in LAM, providing a set of novel potential biomarkers. These understandings may lead to a new effective treatment for LAM in the future. PMID:25133674

Banville, Nessa; Burgess, Janette K; Jaffar, Jade; Tjin, Gavin; Richeldi, Luca; Cerri, Stefania; Persiani, Elisa; Black, Judith L; Oliver, Brian G

2014-01-01

46

Immunosuppressive activity of ethanolic extract of seeds of Moringa oleifera Lam. in experimental immune inflammation  

Microsoft Academic Search

Ethnopharmacological relevanceTraditionally, the plant Moringa oleifera Lam. (Moringaceae) is used for the treatment of ascites and rheumatism, while the dried seeds of the plant are used as an ‘anti-allergic’ agent.

Shailaja G. Mahajan; Anita A. Mehta

2010-01-01

47

[Identification of a confused species of Euphorbia hirta L. E. indica Lam].  

PubMed

The article reported the morphological and histological identification for Euporbia hirta L. and its confused species E. indica Lam.. It provided evidences for identifying Euphorbia hirta L.. PMID:11341025

Chu, X; Cao, L; Yuan, C

2001-01-01

48

Jackfruit (Artocarpus heterophyllus Lam.) Diversity in Bangladesh: Land Use and Artificial Selection1  

E-print Network

Jackfruit (Artocarpus heterophyllus Lam.) Diversity in Bangladesh: Land Use and Artificial, University of Rajshahi, Rajshahi, Bangladesh 3 Chicago Botanic Garden, Glencoe, IL, USA 4 Northwestern University, Plant Biology and Conservation, Evanston, IL, USA 5 Gono University, Savar, Dhaka, Bangladesh

Fant, Jeremie

49

Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats  

Microsoft Academic Search

This study was carried out to evaluate the effect of Moringa oleifera Lam (Moringa) seed extract on liver fibrosis. Liver fibrosis was induced by the oral administration of 20% carbon tetrachloride (CCl4), twice weekly and for 8weeks. Simultaneously, M.oleifera Lam seed extract (1g\\/kg) was orally administered daily. The biochemical and histological results showed that Moringa reduced liver damage as well

Alaaeldin A. Hamza

2010-01-01

50

Benzylcarbamothioethionate from root bark of Moringa oleifera Lam. and its toxicological evaluation (Bencilcarbamotioetionato de la raíz de Moringa oleifera Lam. y su evaluación toxicológica)  

Microsoft Academic Search

A new compound, benzylcarbamothioethionate (1), was isolated from the chloroform soluble fraction of the ethanolic extract of the root bark of Moringa oleifera Lam. Its structure was established on the basis of 1D and 2D-NMR and mass spectroscopy. The acute toxicity studies of chloroform extract and compound 1 were performed on Long Evan's rats using four groups (two controls and

Farjana NIKKON; Sohel HASAN; Kazi Abdus SALAM; Mohammed A. MOSADDIK; Proma KHONDKAR; Mohammed Ekramul HAQUE; Mukhlesur RAHMAN

51

Sulphadimethoxine and Azolla filiculoides Lam.: a model for drug remediation.  

PubMed

Plants can be an interesting tool for in situ remediation of drug contaminated waters. In a laboratory model Azolla filiculoides Lam., an aquatic fern known to absorb pollutants, has been exposed to an environmental persistent antibiotic commonly used in intensive farming, sulphadimethoxine (S), to test its bioremediation capability. In a 5 week experiment, plants were cultivated outdoor at four drug concentrations (50, 150, 300 and 450 mg l(-1)) in N-free mineral medium. Drug affects growth rate (as biomass yield per week), N2-fixation, heterocyst frequency, but plants are able to survive. Notwithstanding, at all concentrations tested drug was actively removed from the medium and the accumulation in the biomass is in order of magnitude up to mg g(-1) plant dry weight (1000 ppm). Drug uptake and degradation rates increase with S concentrations in the culture medium. The efficacy of the model was very high. These results demonstrated that Azolla can be taken into consideration as a tool for sulphonamides environmental monitoring and decontamination. PMID:12188140

Forni, Cinzia; Cascone, Antonella; Fiori, Maurizio; Migliore, Luciana

2002-07-01

52

New phytoconstituents from the aerial parts of Fumaria parviflora Lam.  

PubMed

Fumaria parviflora Lam. (Fumariaceae) is an annual herb found throughout the world. Traditionally it has great significance in various disorders. In folk medicine of Turkey it is used against hepato-biliary dysfunction and imported from Iran. In Charaka and Sushruta, it is recommended for treatment of fevers, blood disorders, chronic skin diseases, urinary diseases and cough. The compounds were isolated from methanolic extract of the plants by column chromatography using silica gel (60-120 mesh) as stationary phase and structure of the isolated compounds have been established on the basis of spectral data analysis and chemical reactions. Phytochemical investigation of its aerial parts led to the isolation of five new compounds characterized as (5?H,11?H)-8-oxo-homoiridolide (1), n-docosanyl-2-O-?-D-glucopyranosyl salicylate (2), 2-methyl-6-hydroxymethylenedodecan-10-oyl-12, 15-olide14-O-?-D-xylopyranoside (3), 4-oxo-stigmast-5-en-3?-ol-D-glucopyranoside (4) and salicylic acid-O-?-D-xylopyranoside (5) along with the known compounds ?-D-glucopyranosyl hexadecanoate (6) and ?-D-glucopyranosyl- (2 ? 1')-?-D-glucopyranoside (7). The isolated compounds are useful as they will provide essential data and information for the further researchers and development of effective analytical marker for identity, purity and quality control of this traditional plant in future. PMID:24959414

Jameel, Mohammad; Ali, Abuzer; Ali, Mohammed

2014-04-01

53

Prescription of Psychiatric Medications and Polypharmacy in the LAMS Cohort  

PubMed Central

Objective This study evaluated demographic and clinical correlates and predictors of polypharmacy at baseline assessment in the Longitudinal Assessment of Manic Symptoms (LAMS) sample, a cohort of children age six to 12 years at their first outpatient mental health visit at university-affiliated clinics. Methods Use of medications in four classes (mood stabilizers, antidepressants, antipsychotics, and stimulants) was assessed, and the Service Assessment for Children and Adolescents classified lifetime and current use of various services. Analyses examined correlates of the number of medications prescribed and odds of polypharmacy, defined as use of two or more concurrent medications. Results In the total sample, 201 of 698 participants (29%) were prescribed two or more medications. These participants had lower Children's Global Assessment Scale scores, more comorbid disorders, and higher baseline parent-reported mood symptoms than those prescribed no or one medication. White youths were three times as likely as nonwhite youths to be receiving two or more psychotropics, even after adjustment for other demographic and clinical characteristics. Of 262 participants (38% of sample) not being treated with medications, 252 (96%) had a diagnosis of at least one psychiatric disorder (74% had two or more). Conclusions Findings suggest that patients with greater severity and comorbidity were more likely to receive two or more medications. However, 38% of these children with serious disorders were not receiving psychotropic medication at the time of this assessment. Results counter findings suggesting overtreatment with medications of children with psychiatric disorders in the community. PMID:23852186

Kowatch, Robert A.; Youngstrom, Eric A.; Horwitz, Sarah; Demeter, Christine; Fristad, Mary A.; Birmaher, Boris; Axelson, David; Ryan, Neal; Frazier, Thomas W.; Arnold, L. Eugene; Young, Andrea S.; Gill, MaryKay; Findling, Robert L.

2014-01-01

54

New phytoconstituents from the aerial parts of Fumaria parviflora Lam  

PubMed Central

Fumaria parviflora Lam. (Fumariaceae) is an annual herb found throughout the world. Traditionally it has great significance in various disorders. In folk medicine of Turkey it is used against hepato-biliary dysfunction and imported from Iran. In Charaka and Sushruta, it is recommended for treatment of fevers, blood disorders, chronic skin diseases, urinary diseases and cough. The compounds were isolated from methanolic extract of the plants by column chromatography using silica gel (60-120 mesh) as stationary phase and structure of the isolated compounds have been established on the basis of spectral data analysis and chemical reactions. Phytochemical investigation of its aerial parts led to the isolation of five new compounds characterized as (5?H,11?H)-8-oxo-homoiridolide (1), n-docosanyl-2-O-?-D-glucopyranosyl salicylate (2), 2-methyl-6-hydroxymethylenedodecan-10-oyl-12, 15-olide14-O-?-D-xylopyranoside (3), 4-oxo-stigmast-5-en-3?-ol-D-glucopyranoside (4) and salicylic acid-O-?-D-xylopyranoside (5) along with the known compounds ?-D-glucopyranosyl hexadecanoate (6) and ?-D-glucopyranosyl- (2 ? 1?)-?-D-glucopyranoside (7). The isolated compounds are useful as they will provide essential data and information for the further researchers and development of effective analytical marker for identity, purity and quality control of this traditional plant in future. PMID:24959414

Jameel, Mohammad; Ali, Abuzer; Ali, Mohammed

2014-01-01

55

Low Cost Silver Ink RFID Tag Antennas Pavel V. Nikitin, Sander Lam, and K. V. S. Rao  

E-print Network

Low Cost Silver Ink RFID Tag Antennas Pavel V. Nikitin, Sander Lam, and K. V. S. Rao Intermec.nikitin, kvs.rao, sander.lam}@intermec.com In this paper, we discuss silver ink as a low cost option from both silver ink and copper is presented at UHF. Influence of each material on tag performance

Hannaford, Blake

56

Identification of eighteen Berberis species as alternate hosts of Puccinia striiformis f. sp. tritici and virulence variation in the pathogen isolates from natural infection of barberry plants in China.  

PubMed

ABSTRACT The wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) population in China has been reported to be a distinct genetic group with higher diversity than those in many other countries. Genetic recombination in the P. striiformis f. sp. tritici population has been identified with molecular markers but whether sexual reproduction occurs in China is unknown. In this study, we surveyed barberry plants for infection by rust fungi in the stripe rust "hotspot" regions in Gansu, Sichuan, and Shaanxi provinces; collected barberry plants and inoculated plants of 20 Berberis spp. with germinated teliospores under controlled greenhouse conditions for susceptibility to P. striiformis f. sp. tritici; and tested P. striiformis f. sp. tritici isolates obtained from aecia on naturally infected barberry plants on the wheat genotypes used to differentiate Chinese P. striiformis f. sp. tritici races to determine virulence variations. Different Berberis spp. were widely distributed and most surveyed plants had pycnia and aecia of rust fungi throughout the surveyed regions. In total, 28 Berberis spp. were identified during our study. From 20 Berberis spp. tested with teliospores of P. striiformis f. sp. tritici from wheat plants, 18 species were susceptible under greenhouse conditions. Among 3,703 aecia sampled from barberry plants of three species (Berberis shensiana, B. brachypoda, and B. soulieana) under natural infections in Gansu and Shaanxi provinces, four produced P. striiformis f. sp. tritici uredinia on susceptible wheat 'Mingxian 169'. Sequence of the internal transcribed spacer (ITS) regions of the four isolates from barberry shared 99% identity with the P. striiformis f. sp. tritici sequences in the National Center for Biotechnology Information database. The four isolates had virulence patterns different from all previously reported races collected from wheat plants. Furthermore, 82 single-uredinium isolates obtained from the four barberry isolates had high virulence diversity rates of 9.0 to 28.1%, indicating that the diverse isolates were produced through sexual reproduction on barberry plants under natural conditions. In addition to P. striiformis f. sp. tritici, sequence analysis of polymerase chain reaction products of the ITS regions and inoculation tests on wheat identified P. graminis (the stem rust pathogen). Our results indicated that P. striiformis f. sp. tritici can infect some Berberis spp. under natural conditions, and the sexual cycle of the fungus may contribute to the diversity of P. striiformis f. sp. tritici in China. PMID:23514262

Zhao, Jie; Wang, Long; Wang, Zhiyan; Chen, Xianming; Zhang, Hongchang; Yao, Juanni; Zhan, Gangming; Chen, Wen; Huang, Lili; Kang, Zhensheng

2013-09-01

57

Discovery of an allosteric site in the caspases Jeanne A. Hardy, Joni Lam, Jack T. Nguyen  

E-print Network

Discovery of an allosteric site in the caspases Jeanne A. Hardy, Joni Lam, Jack T. Nguyen , Tom O, to study allosteric transitions in proteins, and to nucleate drug discovery efforts. Caspases are highly that have frustrated drug discovery efforts (3­7), so despite their biological significance in cell death

Hardy, Jeanne

58

Effects of light and temperature on net primary productivity of fodder galega (galega orientalis lam.)  

Microsoft Academic Search

The effects of light and temperature on the net primary productivity (NPP) of fodder galega (G. orientalis Lam.) were studied in two series of experiments. NPP was determined under laboratory conditions using a stepwise change in photosynthetic photon flux density (PPFD) from 0.0 ?mol m s to 1.21 × 10 ?mol m s at temperatures of 5, 15, 18, 25

T. Aniszewski; S. N. Drozdov; E. S. Kholoptseva; V. K. Kurets; L. A. Obshatko; E. G. Popov; A. V. Talanov

1998-01-01

59

Catching Old Influenza Virus with A New Markov Model HamChing Lam  

E-print Network

Catching Old Influenza Virus with A New Markov Model HamChing Lam Dept. of Computer Science on the Hemag- glutinin (HA) gene, a major surface antigen of the avian influenza virus. Using this model we Influenza virus, Poisson process, Markov Model 1. INTRODUCTION For the past century researchers have been

Boley, Daniel

60

Analyze Influenza Virus Sequences Using Binary Encoding HamChing Lam  

E-print Network

Analyze Influenza Virus Sequences Using Binary Encoding Approach HamChing Lam Dept. of Computer 55455, USA boley@cs.umn.edu ABSTRACT Capturing mutation patterns of each individual influenza virus distance. We first convert the influenza genetic sequence to a binary string and then ap- ply Principal

Boley, Daniel

61

Acumulação de nutrientes em mudas de moringa ( Moringa oleifera Lam) sob omissão de macronutrientes1  

Microsoft Academic Search

Resumo - A Moringa oleifera Lam. é uma espécie arbórea pertencente à família Moringaceae, adaptada às condi- ções áridas e semi-áridas e de uso diversificado com especial destaque na ornamentação de parques e jardins, na alimentação animal, na complementação alimentar humana e na medicina. Uma vez que são poucas as informações sobre essa planta, o trabalho teve como objetivo avaliar

Hugo Vieira; Lucia Helena; Garófalo Chaves; Ricardo Almeida Viégas

2008-01-01

62

CRESCIMENTO INICIAL DE MORINGA ( Moringa oleifera Lam) SOB OMISSÃO DE NUTRIENTES  

Microsoft Academic Search

Moringa oleifera Lam is a leguminous species well adapted to arid and semi arid conditions and is largely used as ornamental for parks and gardens in the animal feeding and human diet and as medicinal. Since research informations on this plant species are scarce, the current work aimed to evaluate the production of leaves, stems and roots dry matter in

Hugo Vieira; Lucia Helena; Garófalo Chaves; Ricardo Almeida Viégas

63

New ?-glucosides of caffeoyl quinic acid from the leaves of Moringa oleifera Lam.  

PubMed

Two new caffeoyl quinic acid ?-glucosides, together with three known caffeoyl quinic acids and five known flavonoid glucosides, were isolated from the leaves of Moringa oleifera Lam. The structures of the new compounds were elucidated as 4-O-(4'-O-?-D-glucopyranosyl)-caffeoyl quinic acid (1) and 4-O-(3'-O-?-D-glucopyranosyl)-caffeoyl quinic acid (2) by spectroscopic analyses. PMID:21748486

Kashiwada, Yoshiki; Ahmed, Fakhruddin Ali; Kurimoto, Shin-ichiro; Kim, Sang-Yong; Shibata, Hirofumi; Fujioka, Toshihiro; Takaishi, Yoshihisa

2012-01-01

64

Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione level  

Microsoft Academic Search

Initiation of acetaminophen (APAP) toxicities is believed to be promoted by oxidative stress during the event of overdosage. The aim of the present study was to evaluate the hepatoprotective action of Moringa oleifera Lam (MO), an Asian plant of high medicinal value, against a single high dose of APAP. Groups of five male Sprague–Dawley rats were pre-administered with MO (200

S. Fakurazi; I. Hairuszah; U. Nanthini

2008-01-01

65

QTL analysis of lodging resistance and related traits in Italian ryegrass ( Lolium multiflorum Lam.)  

Microsoft Academic Search

Italian ryegrass ( Lolium multiflorum Lam.) is the most widely cultivated annual forage grass in Japan. Lodging damage reduces both harvested yield and forage quality. To identify the chromosomal regions controlling lodging resistance in Italian ryegrass, we analyzed seven quantitative characters—heading date, plant height, culm weight, culm diameter, culm strength, tiller number, and culm pushing resistance—and evaluated lodging scores in

Maiko Inoue; Zhensheng Gao; Hongwei Cai

2004-01-01

66

How does nitrogen availability alter rhizodeposition in Lolium multiflorum Lam. during vegetative growth?  

Microsoft Academic Search

The objective of this work was to determine if the impact of nitrogen (N) on the release of organic carbon (C) into the soil\\u000a by roots (rhizodeposition) correlated with the effect of this nutrient on some variables of plant growth. Lolium multiflorum Lam. was grown at two levels of N supply, either in sterile sand percolated with nutrient solution or

Frédéric Henry; Christophe Nguyen; Eric Paterson; Allan Sim; Christophe Robin

2005-01-01

67

Two-step Centrifugal Partition Chromatography (CPC) fractionation of Butea monosperma (Lam.) biomarkers  

Microsoft Academic Search

Butea monosperma (Lam.) Taubert (Synonym Butea frondosa; family Fabaceae) is a popular plant used in traditional medicine in the Indian continent. The brightly orange flowers of this plant are described with antioxidant potential. The aim of this study was to develop a bio-guided fractionation methodology in order to identify antioxidant biomarkers from B. monosperma flowers which could be used to

Thomas Michel; Emilie Destandau; Virginie Pecher; Isabelle Renimel; Laure Pasquier; Patrice André; Claire Elfakir

2011-01-01

68

Bionic lightweight design by laser additive manufacturing (LAM) for aircraft industry  

Microsoft Academic Search

Today many challenges lie ahead of the aircraft industry. The increasing competition and shortage of resources raise a challenge for future manufacturing technologies and lightweight design. A possibility to cope with these circumstances is the manufacturing technology of Laser Additive Manufacturing (LAM). However there are still challenges to cope with due to the processes novelty, such as the development of

Claus Emmelmann; Maren Petersen; Jannis Kranz; Eric Wycisk

2011-01-01

69

NOPdb: Nucleolar Proteome Database Anthony Kar Lun Leung*, Laura Trinkle-Mulcahy, Yun Wah Lam,  

E-print Network

NOPdb: Nucleolar Proteome Database Anthony Kar Lun Leung*, Laura Trinkle-Mulcahy, Yun Wah Lam, Jens Odense M, Denmark Received August 13, 2005; Accepted August 16, 2005 ABSTRACT The Nucleolar Proteome nucleolar proteins and thereby suggests putative functions for factors within the 30% of the proteome which

Trinkle-Mulcahy, Laura

70

A Survey of Data Breakpoint and Reverse Execution Lap Chung Lam  

E-print Network

A Survey of Data Breakpoint and Reverse Execution Lap Chung Lam lclam@cs.sunysb.edu ABSTRACT A debugger is an essential tool for software development because debugging accounts for 80% of the overall software development time. However, relatively less research is done on debuggers as compared to other

Chiueh, Tzi-cker

71

Removal of cadmium from aqueous system by shelled Moringa oleifera Lam. seed powder  

Microsoft Academic Search

The present study explores the unexploited sorption properties of the plant Moringa oleifera Lam. for decontamination of Cd at laboratory scale. Sorption studies using standard practices were carried out in batch experiments as functions of biomass dosage, contact time, metal concentrations, particle size and pH. Percentage sorption in each case was computed on the basis of Cd estimation using a

Parul Sharma; Pushpa Kumari; M. M. Srivastava; Shalini Srivastava

2006-01-01

72

Effect of Moringa oleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats  

Microsoft Academic Search

In India, drumstick (Moringa oleifera Lam. (Moringaceae)) is commonly used as a phytotherapeutic agent. The effect of oral administration of aqueous and alcoholic extract of Moringa oleifera root-wood on calcium oxalate urolithiasis has been studied in male Wistar albino rats. Ethylene glycol feeding resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. Supplementation with aqueous and

Ravindra V. Karadi; Navneet B. Gadge; K. R. Alagawadi; Rudraprabhu V. Savadi

2006-01-01

73

Antifungal activity of crude extracts and essential oil of Moringa oleifera Lam  

Microsoft Academic Search

Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medicines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum, and Microsporum canis. GC–MS analysis of the chemical composition of the essential oil from leaves showed a total of 44 compounds. Isolated

Ping-Hsien Chuang; Chi-Wei Lee; Jia-Ying Chou; M. Murugan; Bor-Jinn Shieh; Hueih-Min Chen

2007-01-01

74

A Co-Training Framework for Searching XML Wilfred Ng and Lau Ho Lam  

E-print Network

A Co-Training Framework for Searching XML Documents Wilfred Ng and Lau Ho Lam Department}@cs.ust.hk Abstract In this paper, we study the use of XML tagged keywords (or simply key-tags) to search an XML as feedback and then to generate an adap- tive ranked list of XML fragments as the search results. First, we

Ng, Wilfred Siu Hung

75

A Multi-Ranker Model for Adaptive XML Searching Ho Lam Lau and Wilfred Ng  

E-print Network

A Multi-Ranker Model for Adaptive XML Searching Ho Lam Lau and Wilfred Ng Department of Computer needs and preferences in searching XML data. In this paper, we employ a list of simple XML tagged keywords as a vehicle for searching XML fragments in a collection of XML documents. In order to deal

Ng, Wilfred Siu Hung

76

mTOR Activation, Lymphangiogenesis, and Estrogen-Mediated Cell Survival: The "Perfect Storm" of Pro-Metastatic Factors in LAM Pathogenesis  

PubMed Central

Abstract Research interest in lymphangioleiomyomatosis (LAM) has grown dramatically in the past decade, particularly among cancer biologists. There are at least two reasons for this: first, the discovery in the year 2000 that LAM cells carry TSC2 gene mutations, linking LAM with cellular pathways including the PI3K/Akt/mTOR axis, and allowing the Tuberous Sclerosis Complex (TSC)-regulated pathways that are believed to underlie LAM pathogenesis to be studied in cells, yeast, Drosophila, and mice. A second reason for the rising interest in LAM is the discovery that LAM cells can travel to the lung, including repopulating a donor lung after lung transplantation, despite the fact that LAM cells are histologically benign. This “benign metastasis” underpinning suggests that elucidating LAM pathogenesis will unlock a set of fundamental mechanisms that underlie metastatic potential in the context of a cell that has not yet undergone malignant transformation. Here, we will outline the data supporting the metastatic model of LAM, consider the biochemical and cellular mechanisms that may enable LAM cells to metastasize, including both cell autonomous and non-cell autonomous factors, and highlight a mouse model in which estrogen promotes the metastasis and survival of TSC2-deficient cells in a MEK-dependent manner. We propose a multistep model of LAM cell metastasis that highlights multiple opportunities for therapeutic intervention. Taken together, the metastatic behavior of LAM cells and the involvement of tumor-related signaling pathways lead to optimism that cancer-related paradigms for diagnosis, staging, and therapy will lead to therapeutic breakthroughs for women living with LAM. PMID:20235886

Yu, Jane

2010-01-01

77

A model of maltodextrin transport through the sugar-specific porin, LamB, based on deletion analysis.  

PubMed Central

LamB facilitates the uptake of maltose and maltodextrins across the bacterial outer membrane and acts as a general porin for small molecules. Using directed deletion mutagenesis we removed several regions of the LamB polypeptide and identified a polypeptide loop that both constricts the maltoporin channel and binds maltodextrins. In conjunction with a second sugar binding site that we identified at the rim of the channel, these data clarify, for the first time, the mechanism of transport through a substrate-specific porin. Furthermore, unlike the transverse loops of general porins, which originate from a central location in their primary structure, the loop that regulates LamB permeability originates from a C-terminal site. Thus LamB represents a second distinct class of porins in the bacterial outer membrane that is differently organized and separately evolved from OmpF-type, general porins. Images PMID:7925308

Klebba, P E; Hofnung, M; Charbit, A

1994-01-01

78

A model of maltodextrin transport through the sugar-specific porin, LamB, based on deletion analysis.  

PubMed

LamB facilitates the uptake of maltose and maltodextrins across the bacterial outer membrane and acts as a general porin for small molecules. Using directed deletion mutagenesis we removed several regions of the LamB polypeptide and identified a polypeptide loop that both constricts the maltoporin channel and binds maltodextrins. In conjunction with a second sugar binding site that we identified at the rim of the channel, these data clarify, for the first time, the mechanism of transport through a substrate-specific porin. Furthermore, unlike the transverse loops of general porins, which originate from a central location in their primary structure, the loop that regulates LamB permeability originates from a C-terminal site. Thus LamB represents a second distinct class of porins in the bacterial outer membrane that is differently organized and separately evolved from OmpF-type, general porins. PMID:7925308

Klebba, P E; Hofnung, M; Charbit, A

1994-10-01

79

Estradiol and tamoxifen stimulate LAM-associated angiomyolipoma cell growth and activate both genomic and nongenomic signaling pathways.  

PubMed

Lymphangioleiomyomatosis (LAM) is a progressive lung disease affecting almost exclusively women. The reasons for this strong gender predisposition are poorly understood. Renal angiomyolipomas occur in 50-60% of sporadic LAM patients. The smooth muscle cells of pulmonary LAM and renal angiomyolipomas are nearly indistinguishable morphologically. Here, we report the first successful cell culture of a LAM-associated renal angiomyolipoma. The cells carried inactivating mutations in both alleles of the TSC2 gene and expressed estrogen receptor , estrogen receptor , and androgen receptor. To elucidate the cellular pathways through which steroid hormones influence LAM pathogenesis, we treated the cells with both estradiol and tamoxifen. Cell growth was stimulated by estradiol, associated with phosphorylation of p44/42 MAPK at 5 min and an increase in c-myc expression at 4 h. Tamoxifen citrate also stimulated cell growth, associated with increased phosphorylation of p44/42 MAPK and expression of c-myc, indicating that tamoxifen has agonist effects on angiomyolipoma cells. This response to tamoxifen in human angiomyolipoma cells differs from prior studies of Eker rat leiomyoma cells, possibly reflecting cell type or species differences in cells lacking tuberin. Our data provide the first evidence that estradiol stimulates the growth of angiomyolipoma cells, that tamoxifen has agonist effects in angiomyolipoma cells, and that estradiol and tamoxifen impact both genomic and nongenomic signaling pathways in angiomyolipoma cells. The responsiveness of angiomyolipoma cells to estradiol may be related to the underlying reasons that LAM affects primarily women. PMID:12922981

Yu, Jane; Astrinidis, Aristotelis; Howard, Sharon; Henske, Elizabeth Petri

2004-04-01

80

Economic evaluation of micafungin vs. liposomal amphotericin B (LAmB) for the treatment of candidaemia and invasive candidiasis (IC).  

PubMed

Micafungin was non-inferior to liposomal amphotericin B (LAmB) for the treatment of candidaemia and invasive candidiasis (IC) in a major clinical trial. The present study investigated the economic impact of micafungin vs. LAmB in treating candidaemia and IC. A decision analytical model was constructed to capture downstream consequences of using micafungin or LAmB as primary definitive therapy. The main outcomes were treatment success and treatment failure due to mycological persistence, or death. Outcome probabilities were derived from key published sources. Resource used was estimated by an expert panel and cost inputs were from the latest Australian resources. The analysis was from an Australian hospital perspective. Sensitivity analyses using Monte Carlo simulation were conducted. Micafungin (AU$61 426) had a lower total cost than LAmB (AU$72 382), with a total net cost-saving of AU$10 957 per patient. This was primarily due to the lower cost associated with initial antifungal treatment and shorter length of stay for patients in the micafungin arm. Hospitalisation was the main cost driver for both arms. Results were robust over a wide range of variables. The uncertainty analysis demonstrated that micafungin had a 99.9% chance of being cost-saving compared with LAmB. Micafungin was associated with cost-saving relative to LAmB in the treatment of candidaemia and IC in Australia. PMID:23496163

Neoh, Chin Fen; Liew, Danny; Slavin, Monica A; Marriott, Debbie; Chen, Sharon C-A; Morrissey, Orla; Stewart, Kay; Kong, David C M

2013-09-01

81

Bionic lightweight design by laser additive manufacturing (LAM) for aircraft industry  

NASA Astrophysics Data System (ADS)

Today many challenges lie ahead of the aircraft industry. The increasing competition and shortage of resources raise a challenge for future manufacturing technologies and lightweight design. A possibility to cope with these circumstances is the manufacturing technology of Laser Additive Manufacturing (LAM). However there are still challenges to cope with due to the processes novelty, such as the development of further materials, especially lightweight alloys, and new design approaches. Therefore innovative approaches for material development and lightweight design were created in order to fully exploit the processes potentials. The material development process is based on an analytical calculation of temperature distribution versus effective process factors in order to identify acceptable operating conditions for the LAM process. A novel approach to extreme lightweight design was realized by incorporating structural optimization tools and bionic structures into one design process. By consequently following these design principles, designers can achieve lightweight savings in designing new aircraft structure and push lightweight design to new limits.

Emmelmann, Claus; Petersen, Maren; Kranz, Jannis; Wycisk, Eric

2011-05-01

82

Localization of iron-superoxide dismutase in the cyanobiont of Azolla filiculoides Lam  

Microsoft Academic Search

Summary Immunogold labeling and transmission electron microscopy were used to localize iron-superoxide dismutase (Fe-SOD) in the different cells of nitrogen-fixing cyanobacterial symbiont present within different leaf cavity groups ofAzolla filiculoides Lam. As evidenced by Western blotting and immunoprecipitation, Fe-SOD antibody fromAnabaena cylindrica recognized Fe-SOD in extracts of the cyanobiont and showed the same electrophoretic mobility and pattern as purifiedA. cylindrica

A. Canini; B. Bergman; P. Civitareale; G. Rotilio; M. Grilli Caiola

1992-01-01

83

On the ecology of Azolla filiculoides Lam. in Damietta district, Egypt  

Microsoft Academic Search

Azolla filiculoidesLam. is a tiny aquatic fern growing as free floating mats covering the stagnant water of the irrigation and drainage canals of Damietta district, Egypt. Ecological characteristics and distribution of the fern were investigated and results were discussed.Vegetation analysis of Azolla indicate that the most common associated species with Azolla are: Phragmites australis [presence (P) = 93.3%], Echinochloa stagnina

Mamdouh S. Serag; Adel El-Hakeem; M. Badway; Mohamed A. Mousa

2000-01-01

84

Volatile components, antioxidant and antibacterial activities of Dacryodes buettneri H. J. Lam. essential oil from Gabon  

Microsoft Academic Search

The water-distilled oil obtained from resin of Dacryodes buettneri H.J.Lam, Burseraceae from Gabon was examined by GC and GC\\/MS. The analysis led to the identification of 28 components. The essential oil is characterized by a high proportion of terpinen-4-ol (27.33%) along with p-cymene (19%), ?-pinene (13.23%), sabinene (4.40%) and isoascaridol (4%). The in vitro antioxidant activity was investigated with two

Louis-Clément Obame; Jean Koudou; Jean-Claude Chalchat; Ismael Bassolé; Prosper Edou; Aboubakar S. Ouattara; Alfred S. Traore

85

‘Drumstick tree’ ( Moringa oleifera Lam.): a multipurpose potential species in India  

Microsoft Academic Search

Moringa oleifera Lam., a medium sized tree species has gained importance due to its multipurpose usage and well adaptability to dry and hot\\u000a climates of north-western plains, central India and dry regions of peninsular India. This species is reported in this paper\\u000a for distribution of diversity and genetic resources value in different parts of the country. Information on diversification\\u000a in

Anjula Pandey; K. Pradheep; Rita Gupta; E. Roshini Nayar; D. C. Bhandari

2011-01-01

86

AntiArthritic Activity of Hydroalcoholic Extract of Flowers of Moringa oleifera Lam. in Wistar Rats  

Microsoft Academic Search

Moringa oleifera Lam. (Moringaceae), commonly known as moringa, drumstick, and horseradish, is a small, fast-growing ornamental tree that originates from India. In the present study, the anti-arthritic activity of a hydroalcoholic extract of Moringa flowers (MOFE, an herbal remedy) was investigated in adjuvant induced arthritis in Wistar rats. Decreases in body weight, paw edema volume (primary lesion), inflammation at non-injected

Shailaja G. Mahajan; Anita A. Mehta

2009-01-01

87

New ?-glucosides of caffeoyl quinic acid from the leaves of Moringa oleifera Lam  

Microsoft Academic Search

Two new caffeoyl quinic acid ?-glucosides, together with three known caffeoyl quinic acids and five known flavonoid glucosides,\\u000a were isolated from the leaves of Moringa oleifera Lam. The structures of the new compounds were elucidated as 4-O-(4?-O-?-d-glucopyranosyl)-caffeoyl quinic acid (1) and 4-O-(3?-O-?-d-glucopyranosyl)-caffeoyl quinic acid (2) by spectroscopic analyses.

Yoshiki KashiwadaFakhruddin; Fakhruddin Ali Ahmed; Shin-ichiro Kurimoto; Sang-Yong Kim; Hirofumi Shibata; Toshihiro Fujioka; Yoshihisa Takaishi

88

Anti-fungal activity of crude extracts and essential oil of Moringa oleifera Lam.  

PubMed

Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medicines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum, and Microsporum canis. GC-MS analysis of the chemical composition of the essential oil from leaves showed a total of 44 compounds. Isolated extracts could be of use for the future development of anti-skin disease agents. PMID:16406607

Chuang, Ping-Hsien; Lee, Chi-Wei; Chou, Jia-Ying; Murugan, M; Shieh, Bor-Jinn; Chen, Hueih-Min

2007-01-01

89

Biochemical properties of metalloproteinases from the hemolymph of the mussel Mytilus galloprovincialis Lam  

Microsoft Academic Search

The expression of matrix metalloproteinases (MMP) with gelatinase activity was found in the whole hemolymph of the marine mussel Mytilus galloprovincialis Lam. Cleavage activity was specific for gelatin; very little activity towards human type-IV collagen, and no activity for cold fish gelatin, casein or bovine serum albumin were detected. EDTA and 1,10-phenanthroline were inhibitory, suggesting that mussel MMPs require divalent

Ferdinando Mannello; Laura Canesi; Giancarlo Gazzanelli; Gabriella Gallo

2001-01-01

90

In vitro hypoglycemic effects of Butea monosperma Lam. leaves and bark  

Microsoft Academic Search

The present study evaluated the antidiabetic potential of Butea monosperma Lam. Kuntze (Fabaceae) leaves and the stem bark using various in vitro techniques. The samples were studied for their effects\\u000a on glucose adsorption, diffusion, amylolysis kinetics, enteric enzymes and glucose transport across yeast cells. Both the\\u000a samples adsorbed glucose and the adsorption of glucose increased with increase in glucose concentration.

M. Harish; Faiyaz Ahmed; Asna Urooj

91

Semi-viviparous embryo development and dehydrin expression in the mangrove Rhizophora mucronata Lam  

Microsoft Academic Search

Rhizophora mucronata Lam. is a tropical mangrove with semi-viviparous (cotyledon body protrusion before shedding), non-quiescent and non-desiccating\\u000a (recalcitrant) seeds. As recalcitrance has been thought to relate to the absence of desiccation-related proteins such as dehydrins,\\u000a we for the first time systematically described and classified embryogenesis in R. mucronata and assessed the presence of dehydrin-like proteins. Embryogenesis largely follows the classic

Flora AbdulRahman Ismail; Lisette M. C. Nitsch; Mieke M. C. Wolters-Arts; Celestina Mariani; Jan W. M. Derksen

2010-01-01

92

Establishment of Carex stricta Lam. seedlings in experimental wetlands with implications for restoration  

Microsoft Academic Search

The loss of Carex dominated meadows due to agricultural drainage in the previously glaciated midcontinent of North America has been extensive.\\u000a The lack of natural Carex recruitment in wetland restorations and the failures of revegetation attempts underscore the need for information on the\\u000a establishment requirements of wetland sedges. In this study, seedlings of Carex stricta Lam. were planted in three

Rachel A. Budelsky; Susan M. Galatowitsch

2004-01-01

93

Isolation and characterization of a flocculating protein from Moringa oleifera Lam  

Microsoft Academic Search

A flocculating protein from the seeds of Moringa oleifera Lam. was isolated by extraction with phosphate buffer followed by cation exchange chromatography. The molecular mass of the protein determined by SDS-PAGE was about 6.5 kDa, the isoelectric point was above pH 10. Amino acid analysis and sequencing showed high contents of glutamine, arginine and proline, and a total of 60

Ursula Gassenschmidt; Klaus D. Jany; Tauscher Bernhard; Heinz Niebergall

1995-01-01

94

RETINA: A REal-time TraffIc NAvigation System Kam-Yiu Lam1  

E-print Network

1 RETINA: A REal-time TraffIc NAvigation System Kam-Yiu Lam1 , Edward Chan1 , Tei-Wei Kuo2 , S.W Ng In this demonstration paper, the prototype called REal-time TraffIc NAvigation System (RETINA) is introduced. The goal. In this paper, we introduce a system prototype called RETINA, which is an abbreviation of REal-time Traff

Chan, Edward

95

Simulation of in-cloud icing events on Mount Washington with the GEM-LAM  

NASA Astrophysics Data System (ADS)

In-cloud icing on structures such as transmission lines and wind turbines is an important consideration both for design and operations. It often occurs in coastal areas and over high terrain, where there are virtually no systematic observations. The regional mesoscale model GEM-LAM of the Canadian Meteorological Center (CMC) was used to model three historical icing events on Mount Washington, where observational data were available. These three events are representative of the most frequent low level wind directions for seven available observation periods. A newly developed sophisticated two-moment microphysics scheme (Milbrandt-Yau) is used in GEM-LAM. The simulated cloud properties and other meteorological data are compared with near surface observational data. Simulation results from the 1-km resolution run agree best with the observations, with an average RMSE (root mean square error) of 1.6°C for near surface temperature, 4.6 m s-1 for wind speed, 0.23 g m-3 for liquid water content, and 5.8 ?m for the median volume droplet diameter. These simulated meteorological fields and cloud properties were used as inputs to a cylindrical sleeve icing model. The modeled icing rate from the GEM-LAM simulated fields follows the temporal evolution of the observed one with average RMSE of 1.53 g m-1 min-1 compared to an average measured icing rate of 1.98 g m-1 min-1 for all the three cases.

Yang, Jing; Jones, Kathleen F.; Yu, Wei; Morris, Robert

2012-09-01

96

Interaction of Bacteriophage ? with Its E. coli Receptor, LamB  

PubMed Central

The initial step of viral infection is the binding of a virus onto the host cell surface. This first viral-host interaction would determine subsequent infection steps and the fate of the entire infection process. A basic understating of the underlining mechanism of initial virus-host binding is a prerequisite for establishing the nature of viral infection. Bacteriophage ? and its host Escherichia coli serve as an excellent paradigm for this purpose. ? phages bind to specific receptors, LamB, on the host cell surface during the infection process. The interaction of bacteriophage ? with the LamB receptor has been the topic of many studies, resulting in wealth of information on the structure, biochemical properties and molecular biology of this system. Recently, imaging studies using fluorescently labeled phages and its receptor unveil the role of spatiotemporal dynamics and divulge the importance of stochasticity from hidden variables in the infection outcomes. The scope of this article is to review the present state of research on the interaction of bacteriophage ? and its E. coli receptor, LamB. PMID:23202520

Chatterjee, Sujoy; Rothenberg, Eli

2012-01-01

97

Antidiabetic Effect of Methanolic Extract from Berberis julianae Schneid. via Activation of AMP-Activated Protein Kinase in Type 2 Diabetic Mice.  

PubMed

We have investigated the antidiabetic effect and mechanism of methanolic extract of Berberis julianae Schneid. (BJSME) in STZ induced Type 2 diabetes mellitus mice. T2DM mice were induced by high fat diet and low dose streptozotocin (STZ). BJSME was orally administrated at the doses of 60, 120, and 240?mg/kg/d, for 21 days. Metformin was used as positive control drug. Food intake, body weight, plasma glucose, oral glucose tolerance test, insulin tolerance test, insulin, and blood-lipid content were measured. The effects of BJSME on the glucose transporter 4 (GLUT4) translocation in L6 myotubes and the GLUT4 protein expression in skeletal muscle as well as phosphorylation of the AMP-activated protein kinase (AMPK) in liver and muscle were examined. In vitro and in vivo results indicate that BJSME increased GLUT4 translocation by 1.8-fold and BJSME significantly improved the oral glucose tolerance and low density lipoprotein cholesterol (LDL-C) of serum and reduced body weight, glucose, and other related blood-lipid contents. The BJSME treatment also stimulated the phosphorylation of AMPK. Thus, BJSME seems to possess promising beneficial effects for the treatment of T2DM with the possible mechanism via stimulating AMPK activity. PMID:25258641

Yang, Jing; Zhao, Ping; Wan, Dingrong; Zhou, Qi; Wang, Chao; Shu, Guangwen; Mei, Zhinan; Yang, Xinzhou

2014-01-01

98

Effect of berberine and Berberis aetnensis C. Presl. alkaloid extract on glutamate-evoked tissue transglutaminase up-regulation in astroglial cell cultures.  

PubMed

Berberis aetnensis C. Presl. is a bushy-spiny shrub common on Mount Etna (Sicily, Italy), containing various alkaloids with several pharmacological properties. This study assessed the effect of berberine and of the alkaloid extract of B. aetnensis roots on the glutamate-evoked tissue transglutaminase (TG2) up-regulation in rat astrocyte primary cultures, used as an in vitro model of excitotoxicity. The findings show that the alkaloid extract of B. aetnensis roots consists mainly of berberine. Furthermore, berberine and the alkaloid extract of B. aetnensis roots were able to restore the oxidative status modified by glutamate and the levels of TG2 to control values. It was found that berberine or the alkaloid extract of B. aetnensis roots are able to ameliorate the excessive production of glutamate, protein misfolding and aggregation, mitochondrial fragmentation, and neurodegeneration. Thus, it is suggested that berberine and the alkaloid extract of B. aetnensis roots, may represent a natural therapeutic strategy in the neuropathological conditions associated with excitotoxicity. PMID:21086546

Campisi, Agata; Acquaviva, Rosaria; Mastrojeni, Silvana; Raciti, Giuseppina; Vanella, Angelo; De Pasquale, Rita; Puglisi, Salvatore; Iauk, Liliana

2011-06-01

99

Antidiabetic Effect of Methanolic Extract from Berberis julianae Schneid. via Activation of AMP-Activated Protein Kinase in Type 2 Diabetic Mice  

PubMed Central

We have investigated the antidiabetic effect and mechanism of methanolic extract of Berberis julianae Schneid. (BJSME) in STZ induced Type 2 diabetes mellitus mice. T2DM mice were induced by high fat diet and low dose streptozotocin (STZ). BJSME was orally administrated at the doses of 60, 120, and 240?mg/kg/d, for 21 days. Metformin was used as positive control drug. Food intake, body weight, plasma glucose, oral glucose tolerance test, insulin tolerance test, insulin, and blood-lipid content were measured. The effects of BJSME on the glucose transporter 4 (GLUT4) translocation in L6 myotubes and the GLUT4 protein expression in skeletal muscle as well as phosphorylation of the AMP-activated protein kinase (AMPK) in liver and muscle were examined. In vitro and in vivo results indicate that BJSME increased GLUT4 translocation by 1.8-fold and BJSME significantly improved the oral glucose tolerance and low density lipoprotein cholesterol (LDL-C) of serum and reduced body weight, glucose, and other related blood-lipid contents. The BJSME treatment also stimulated the phosphorylation of AMPK. Thus, BJSME seems to possess promising beneficial effects for the treatment of T2DM with the possible mechanism via stimulating AMPK activity. PMID:25258641

Yang, Jing; Zhao, Ping; Wan, Dingrong; Zhou, Qi; Wang, Chao; Shu, Guangwen; Mei, Zhinan; Yang, Xinzhou

2014-01-01

100

Heterologous expression of two FAD-dependent oxidases with (S)-tetrahydroprotoberberine oxidase activity from Arge mone mexicana and Berberis wilsoniae in insect cells.  

PubMed

Berberine, palmatine and dehydrocoreximine are end products of protoberberine biosynthesis. These quaternary protoberberines are elicitor inducible and, like other phytoalexins, are highly oxidized. The oxidative potential of these compounds is derived from a diverse array of biosynthetic steps involving hydroxylation, intra-molecular C-C coupling, methylenedioxy bridge formation and a dehydrogenation reaction as the final step in the biosynthesis. For the berberine biosynthetic pathway, the identification of the dehydrogenase gene is the last remaining uncharacterized step in the elucidation of the biosynthesis at the gene level. An enzyme able to catalyze these reactions, (S)-tetrahydroprotoberberine oxidase (STOX, EC 1.3.3.8), was originally purified in the 1980s from suspension cells of Berberis wilsoniae and identified as a flavoprotein (Amann et al. 1984). We report enzymatic activity from recombinant STOX expressed in Spodoptera frugiperda Sf9 insect cells. The coding sequence was derived successively from peptide sequences of purified STOX protein. Furthermore, a recombinant oxidase with protoberberine dehydrogenase activity was obtained from a cDNA library of Argemone mexicana, a traditional medicinal plant that contains protoberberine alkaloids. The relationship of the two enzymes is discussed regarding their enzymatic activity, phylogeny and the alkaloid occurrence in the plants. Potential substrate binding and STOX-specific amino acid residues were identified based on sequence analysis and homology modeling. PMID:21327819

Gesell, Andreas; Chávez, Maria Luisa Díaz; Kramell, Robert; Piotrowski, Markus; Macheroux, Peter; Kutchan, Toni M

2011-06-01

101

Clonal expansion and seedling recruitment of Oregon grape (Berberis nervosa) in Douglas-fir (Pseudotsuga menziesii) forests: comparisons with salal (Gaultheria shallon)  

USGS Publications Warehouse

Seedling regeneration and morphology of Oregon grape (Berberis nervosa Pursh) and salal (Gaultheria shallon Pursh) were studied in thinned and unthinned Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) stands in the central Coast Range, Oregon. Above- and below-ground growth of both species were significantly and negatively correlated with stand density. Oregon grape appears to have less potential for vegetative spread than does salal. It produced two to three times fewer rhizome extensions, and rhizome extensions were only half as long as those of salal. Oregon grape seedlings were common in areas of moss ground cover among patches of the two species. Salal seedlings were restricted to decaying logs. Seedling densities of Oregon grape in thinned stands were more than six times those in unthinned stands. For Oregon grape, understory establishment is accomplished by seedling establishment and recruitment of new genets. In contrast, salal maintains itself in forest understories primarily through vegetative growth, since its seedling establishment is restricted mainly to decayed wood. Continual recruitment of new aerial stems or ramets enables Oregon grape to maintain a dense cover once it is established in the understory.

Huffman, D.; Tappeiner, J. C., II

1997-01-01

102

Inhibitory Effect of Berberis vulgaris Aqueous Extract on Acquisition and Reinstatement Effects of Morphine in Conditioned Place Preferences (CPP) in Mice  

PubMed Central

Background: It has been elucidated that Berberis vulgaris (barberry) can alleviate morphine withdrawal syndrome. Also it has been reported that aqueous extract of barberry possibly have inhibitory effect on NMDA receptors. Objectives: In this study, we decided to evaluate the effects of aqueous extract of B. vulgaris fruit on morphine tendency in mice using conditioned place preference (CPP) method. Materials and Methods: In experiment 1 (acquisition phase), mice underwent morphine-induced conditioned place preference (CPP) training with injections of morphine (40 mg/kg). In experiment 2 (extinction and reinstatement phases), mice underwent the same CPP training as in experiment 1 and subsequent extinction training on day 16th a reinstatement by CPP was done by injection of reminding 10 mg/kg morphine. Results: The administration of morphine (40 mg/kg for four days) produced place preference. In the first method, the aqueous extract of barberry (200 mg/kg) prevented morphine tendency to white cell in CPP method. In the second method, after inter-peritoneal injection of aqueous extracts of barberry at 100 and 200 mg/kg, the animals tendency toward the white cells of CPP chamber on the sixteenth day (after a reminder injection of morphine 10 mg/kg) was significantly reduced. Conclusions: These results show that aqueous extract of barberry can reduce the acquisition and reinstatement of morphine-induced conditioned place preference.

Imenshahidi, Mohsen; Qaredashi, Reza; Hashemzaei, Mahmoud; Hosseinzadeh, Hossein

2014-01-01

103

Patrick Lam Patrick received his Ph.D. from the University of Rochester (Dr. Louis Friedrich) and was a postdoctoral fellow in UCLA (Prof. Mike Jung and  

E-print Network

Patrick Lam Patrick received his Ph.D. from the University of Rochester (Dr. Louis Friedrich-discoverer of the powerful Chan-Lam Coupling Reaction. Patrick has worked with CROs successfully and productively for ten/patent applications. Discovery of Eliquis®/Apixaban, a novel Factor Xa inhibitor as a transformational new

Tian, Weidong

104

"Phloem sap analysis of Schleichera oleosa (Lour) Oken, Butea monosperma (Lam) Taub. and Ziziphus mauritiana (Lam) and hemolymph of Kerria lacca (Kerr) using HPLC and tandem mass spectrometry".  

PubMed

Females of lac insects especially of Kerria lacca (Kerr) secret a resin known as lac for their own protection, which has tremendous applications. Lac insect completes its lifecycle on several host taxa where it exclusively feeds on phloem sap but Schleichera oleosa (Lour.) Oken, Butea monosperma (Lam.) and Ziziphus mauritiana (Lam.) are its major hosts. Analysis of phloem sap constituents as well as hemolymph of lac insect is important because it ultimately gets converted into lac by insect intervention. Main phloem sap constituent's viz. sugars and free amino acids and hemolymph of lac insect were analyzed using HPLC and tandem mass spectrometry, respectively. The results were transformed to relative percentage of the total sugars and free amino acids analyzed in each sample for comparison among lac insect hemolymph and the phloem sap of the three different host taxa. Sucrose (58.9?±?3.6-85.6?±?0.9) and trehalose (62.3?±?0.4) were the predominant sugars in phloem sap of three taxa and hemolymph of lac insect, respectively. Glutamic acid (33.1?±?1.4-39.8?±?1.4) was found to be main amino acid among the phloem sap of three taxa while tyrosine (61?±?2.6) was the major amino acid in hemolymph of lac insect. The relative percentage of non-essential amino acids (60.8 %-69.9 %) was found to be more in all the three host taxa while essential amino acids (30.1 %-35.4 %) were present at a lower relative percentage. In contrast to this, the relative percentage of essential amino acids (81.9 %) was observed to be higher as compared to non-essential amino acids (17.7 %) in lac insect hemolymph. These results led to the detection of lac insect's endosymbionts. Moreover, this study revealed a clue regarding the importance of development of a synthetic diet for this insect so that a precise pathway of lac biosynthesis could be investigated for thorough understanding. PMID:24431523

Vashishtha, Amit; Rathi, Brijesh; Kaushik, Sandeep; Sharma, K K; Lakhanpaul, Suman

2013-10-01

105

Anthelmintic effects of Salix babylonica L. and Leucaena leucocephala Lam. extracts in growing lambs.  

PubMed

Twenty Katahdin?×?Pelibuey crossbreed male lambs, 3 to 4 months of age and 24?±?0.3 kg of body weight, were used to study the anthelmintic effects of administering extracts of Salix babylonica L. (SB) and Leucaena leucocephala Lam. (LL). Lambs had not been treated with anthelmintics previously and were randomly allocated into four groups of five lambs each in a completely randomized design. Treatments were as follows: control (lambs fed on total mixed ration without extracts), SB (as control plus S. babylonica L. extract at 30 ml/day), LL (as control plus L. leucocephala Lam. extract at 30 ml/day), and SBLL (as control plus 30 ml/day of S. babylonica L. and L. leucocephala Lam. extracts in a 1:1 (v/v) mixture) for 63 days. Extracts were orally administered before the 8:00 a.m. feeding to each lamb. Rectal fecal samples were collected from each lamb at day 22 (P1), day 43 (P2), and day 63 (P3) of the experiment. Adult worm and egg counts were determined in each fecal sample immediately after collection. Plant secondary metabolites of total phenolics, saponins, and the aqueous fraction were 50 % lower in the SB versus LL extracts. Overall, the oral administration of extracts has improved the egg and worm count reductions in lamb feces by 54, 47, and 40 % for LL, SB, and SBLL, respectively, versus the control lambs. Reductions of worm egg counts in lamb feces were higher (P?

Hernandez, Pablo Mejia; Salem, Abdelfattah Z M; Elghandour, Mona M M Y; Cipriano-Salazar, Moisés; Cruz-Lagunas, Blas; Camacho, Luis Miguel

2014-01-01

106

Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.  

PubMed

We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C3 neutralization epitope from poliovirus) into permissive sites of LamB (i.e., sites which tolerate insertions without deleterious effects on the protein activities or the cell). A specific monoclonal antibody was then used to examine the position of the inserted epitope with respect to the protein and the membrane. In the present work, we set up a site-directed procedure to insert the C3 epitope at new sites in order to distinguish between two-dimensional folding models. This allowed us to identify two new surface loops of LamB and to predict another periplasmic exposed region. The results obtained by random and directed epitope tagging are analyzed in light of the recently published X-ray structure of the LamB protein. Study of 23 hybrid LamB-C3 proteins led to the direct identification of five of the nine external loops (L4, L5, L6, L7, and L9) and led to the prediction of four periplasmic loops (I1, I4, I5, and I8) of LamB. Nine of the hybrid proteins did not lead to topological conclusions, and none led to the wrong predictions or conclusions. The comparison indicates that parts of models based on secondary structure predictions alone are not reliable and points to the importance of experimental data in the establishment of outer membrane protein topological models. The advantages and limitations of genetic foreign epitope insertion for the study of integral membrane proteins are discussed. PMID:8655540

Newton, S M; Klebba, P E; Michel, V; Hofnung, M; Charbit, A

1996-06-01

107

Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.  

PubMed Central

We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C3 neutralization epitope from poliovirus) into permissive sites of LamB (i.e., sites which tolerate insertions without deleterious effects on the protein activities or the cell). A specific monoclonal antibody was then used to examine the position of the inserted epitope with respect to the protein and the membrane. In the present work, we set up a site-directed procedure to insert the C3 epitope at new sites in order to distinguish between two-dimensional folding models. This allowed us to identify two new surface loops of LamB and to predict another periplasmic exposed region. The results obtained by random and directed epitope tagging are analyzed in light of the recently published X-ray structure of the LamB protein. Study of 23 hybrid LamB-C3 proteins led to the direct identification of five of the nine external loops (L4, L5, L6, L7, and L9) and led to the prediction of four periplasmic loops (I1, I4, I5, and I8) of LamB. Nine of the hybrid proteins did not lead to topological conclusions, and none led to the wrong predictions or conclusions. The comparison indicates that parts of models based on secondary structure predictions alone are not reliable and points to the importance of experimental data in the establishment of outer membrane protein topological models. The advantages and limitations of genetic foreign epitope insertion for the study of integral membrane proteins are discussed. PMID:8655540

Newton, S M; Klebba, P E; Michel, V; Hofnung, M; Charbit, A

1996-01-01

108

Physicochemical Properties of Caribbean Sweet Potato ( Ipomoea batatas (L) Lam) Starches  

Microsoft Academic Search

The proximate compositions and physicochemical properties of 21 Caribbean sweet potato (Ipomoea batatas (L) Lam) starches were investigated. Proximate composition, amylose, reducing and non-reducing sugars were determined using\\u000a standard methods. Swelling power and solubility were evaluated and pasting properties of the starches were determined using\\u000a Rapid Visco Analyzer. Moisture (8.0–12.4%), protein (0.0–0.2%), ash (0.1–0.5%), and reducing (0.3–2.3%) and non-reducing sugar

Adebisola J. Aina; Kolawole O. Falade; John O. Akingbala; Pathelene Titus

109

Antioxidant action of Moringa oleifera Lam. (drumstick) against antitubercular drugs induced lipid peroxidation in rats.  

PubMed

The protective effect of Moringa oleifera Lam. (Moringaceae) on hepatic marker enzymes, lipid peroxidation, and antioxidants was investigated during antitubercular drug (isoniazid, rifampicin, and pyrazinamide)-induced toxicity in rats. Enhanced hepatic marker enzymes and lipid peroxidation of antitubercular drug treatment was accompanied by a significant decrease in the levels of vitamin C, reduced glutathione, superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase. Administration of Moringa oleifera extract and silymarin significantly decreased hepatic marker enzymes and lipid peroxidation with a simultaneous increase in the level of antioxidants. We speculate that Moringa oleifera extract exerts its protective effects by decreasing liver lipid peroxides and enhancing antioxidants. PMID:14585192

Ashok Kumar, N; Pari, L

2003-01-01

110

Advances in functional use of sweet potato, [Ipomoea batatas (L.) Lam].  

PubMed

This article reviews the patents that have been presented over the past two decades related to alternative functional use of the Sweet potato, Ipomoea batatas (L.) Lam. The major categories of available patents include alternative food products such as Sweet potato chips and fries, Sweet potato ornamental products, and fuel ethanol production from Sweet potato. The majority of recent patents fall under the category of ornamental products and alternative food products, with only a few fuel ethanol products. Figure 1 shows the major categories of patented alternative products from Sweet potato. PMID:22741930

Barnes, Sandra L; Sanders, Sheila A

2012-08-01

111

Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats.  

PubMed

This study was carried out to evaluate the effect of Moringa oleifera Lam (Moringa) seed extract on liver fibrosis. Liver fibrosis was induced by the oral administration of 20% carbon tetrachloride (CCl(4)), twice weekly and for 8 weeks. Simultaneously, M.oleifera Lam seed extract (1g/kg) was orally administered daily. The biochemical and histological results showed that Moringa reduced liver damage as well as symptoms of liver fibrosis. The administration of Moringa seed extract decreased the CCl(4)-induced elevation of serum aminotransferase activities and globulin level. The elevations of hepatic hydroxyproline content and myeloperoxidase activity were also reduced by Moringa treatment. Furthermore, the immunohistochemical study showed that Moringa markedly reduced the numbers of smooth muscle alpha-actin-positive cells and the accumulation of collagens I and III in liver. Moringa seed extract showed significant inhibitory effect on 1,1-diphenyl-2-picrylhydrazyl free radical, as well as strong reducing antioxidant power. The activity of superoxide dismutase as well as the content of both malondialdehyde and protein carbonyl, which are oxidative stress markers, were reversed after treatment with Moringa. Finally, these results suggested that Moringa seed extract can act against CCl(4)-induced liver injury and fibrosis in rats by a mechanism related to its antioxidant properties, anti-inflammatory effect and its ability to attenuate the hepatic stellate cells activation. PMID:19854235

Hamza, Alaaeldin A

2010-01-01

112

[Case of lymphangioleiomyomatosis (LAM) discovered during cesarean section under spinal anesthesia].  

PubMed

We experienced a case of scheduled cesarean section under spinal anesthesia in a patient with LAM which had been missed in spite of preoperative medical examination and consultation with specialists but discovered because of perioperative hypoxia A 35-year-old woman, Gravida 1 Para 0, with breech presentation was scheduled to undergo cesarean section under spinal anesthesia at 38 weeks of gestation. She had no history of asthma or abnormal findings at annual medical examination. She had suffered from dry cough and nocturnal dyspnea for 7 weeks and an inhaled bronchodilator was administered with diagnosis of inflammatory airway disease by her respiratory physicians. Spinal anesthesia was performed with bupivacaine 12.5 mg. At the beginning of anesthesia SPO2 was 97% in supine position, but it rapidly decreased to less than 90% and 3 l x min(-1) oxygen was supplied with a facial mask. The anesthetic level was thoracal 4 bilaterally and her breathing was stable. The circulatory state, Apgar score and other vital signs were within normal ranges. Postoperative chest X-ray showed bilateral numerous grained spots and computed tomography scans showed multiple thin-walled cysts. The characteristic history and the fluoroscopic data gave her clinical diagnosis of LAM. PMID:24601117

Nakanishi, Mika; Okura, Nahomi; Kashii, Tomoko; Matsushita, Mitsuji; Mori, Masanobu; Yoshida, Masayo; Tsujimura, Shigehisa

2014-02-01

113

Analysis of hydroxycinnamic acids derivatives in calafate (Berberis microphylla G. Forst) berries by liquid chromatography with photodiode array and mass spectrometry detection.  

PubMed

Calafate (Berberis microphylla G. Forst) is a Patagonian barberry very rich in anthocyanins and one of the fruits with the highest levels of these polyphenols. Other phenolic compounds have also been described in calafate berries. However, to the best of our knowledge there is no available information on hydroxycinnamic acid derivatives. The complexity of hydroxycinnamic acids determination in calafate berries, due to their structure similarities and the interference of high anthocyanin concentration is addressed by means of solid liquid extraction, followed by solid phase extraction clean-up on MCX columns and HPLC-DAD-ESI-MS/MS. The optimized extraction, clean-up and HPLC separation method allowed the assignation of identity and quantification of 20 hydroxycinnamic acids from calafate fruits. 5-Caffeoylquinic acid was the main compound found in all the studied samples. Other 13 hydroxycinnamoyl quinic acids and 6 caffeic acid esters with aldaric acid derivatives assigned as glucaric acid were also identified. Moreover, the glucaric-based hydroxycinnamic acid derivatives accounted for almost the half of total content of this kind of phenolic compounds. The total concentration of hydroxycinnamic acids derivatives ranged between 0.32±0.00 ?mol/g and 8.28±0.01 ?mol/g. Effect of ripening and geographical location on hydroxycinnamic acid profiles and concentrations are also evaluated. The methodology allows the determination of hydroxycinnamic acids from calafate despite of the high anthocyanin concentrations, showing a much higher concentration of these acids than other widely consumed berries. Thus suggesting that calafate could be considered a very interesting fruit from the point of view of their nutraceutical composition. However, geographical location and ripening have incidence in levels of studied compounds. PMID:23398997

Ruiz, Antonieta; Mardones, Claudia; Vergara, Carola; Hermosín-Gutiérrez, Isidro; von Baer, Dietrich; Hinrichsen, Patricio; Rodriguez, Roberto; Arribillaga, Diego; Dominguez, Erwin

2013-03-15

114

Seasonal variation of temperature response of respiration in invasive Berberis thunbergii (Japanese barberry) and two co-occurring native understory shrubs in a northeastern US deciduous forest.  

PubMed

In the understory of a closed forest, plant growth is limited by light availability, and early leafing is proposed to be an important mechanism of plant invasion by providing a spring C "subsidy" when high light is available. However, studies on respiration, another important process determining plant net C gain, are rare in understory invasive plants. In this study, leaf properties and the temperature response of leaf respiration were compared between invasive Berberis thunbergii, an early leafing understory shrub, and two native shrubs, Kalmia latifolia, a broadleaf evergreen and Vaccinium corymbosum, a late-leafing deciduous species, in an oak-dominated deciduous forest. The seasonal trend of the basal respiration rates (R(0)) and the temperature response coefficient (E(0)), were different among the three shrubs and species-specific negative correlations were observed between R(0) and E(0). All three shrubs showed significant correlation between respiration rate on an area basis (20 degrees C) and leaf N on an area basis. The relationship was attributed to the variation of both leaf N on a mass basis and leaf mass per area (LMA) in B. thunbergii, but to LMA only in K. latifolia and V. corymbosum. After modeling leaf respiration throughout 2004, B. thunbergii displayed much higher annual leaf respiration (mass based) than the two native shrubs, indicating a higher cost per unit of biomass investment. Thus, respiratory properties alone were not likely to lead to C balance advantage of B. thunbergii. Future studies on whole plant C budgets and leaf construction cost are needed to address the C balance advantage in early leafing understory shrubs like B. thunbergii. PMID:17609983

Xu, Cheng-Yuan; Schuster, W S F; Griffin, Kevin L

2007-10-01

115

Evaluation of the effects of hydroalcoholic extract of Berberis vulgaris root on the activity of liver enzymes in male hypercholesterolemic rats  

PubMed Central

Objectives: Hyperlipidemia can cause a variety of diseases such as atherosclerosis, diabetes, and fatty liver which is followed by increased liver enzymes. Since Berberis vulgaris (B. vulgaris) root possesses antioxidant properties, the present study was conducted to investigate the effect of its extract on the activity of liver enzymes in rats. Materials and Methods: In this experimental study, sixty Wistar rats were selected and allocated to six groups of ten each. The control group received a normal diet and the sham group received a fatty diet while the other groups including experimental groups received a fatty diet and the alcoholic extract of B. vulgaris at minimum (75 mg/kg), moderate (150 mg/kg), and maximum (300 mg/kg) doses by intraperitoneal injection (i.p.) or oral atorvastatin (10 mg /kg) with a fatty diet. At the end of this 21-day period, blood samples were drawn and the levels of the intended factors were measured. Data were analyzed using SPSS software version 11.5. Results: The comparison of the obtained results showed that the levels of alanine transaminase (ALT) and alkaline phosphatase (ALP) enzymes in the sham group that only received fatty food increased (p?0.05), whereas in the treatment groups receiving B. vulgaris extract as well as in the group receiving Atorvastatin, these enzymes significantly decreased; however, no significant changes were observed in aspartate transaminase (AST) levels. Conclusion: Noticing the antioxidant properties of B. vulgaris root extract and its effects on reducing the activity of liver enzymes, the extract of this plant can be a good choice for improving the function of liver. PMID:25050245

Taheri, Soheila; Zarei, Ali; Changizi Ashtiyani, Saeed; Rezaei, Azam; Zaheiri, Saeed

2012-01-01

116

The effects of floating mats of Azolla filiculoides Lam. and Lemna minuta Kunth on the growth of submerged macrophytes  

Microsoft Academic Search

In laboratory experiments, the growth characteristics of the submerged species Elodea nuttallii (Planch.) St. John and Potamogeton crispus L. were assessed in the presence and absence of floating mats of Azolla filiculoides Lam. and Lemna minuta Kunth. Light penetration and the development of pH and dissolved oxygen differences were monitored. The growth of P. crispus was suppressed much more than

Rachel A. Janes; John W. Eaton; Keith Hardwick

1996-01-01

117

ReTAX+: A Cooperative Taxonomy Revision Tool Sik Chun (Joey) Lam, Derek Sleeman, and Wamberto Vasconcelos  

E-print Network

1 ReTAX+: A Cooperative Taxonomy Revision Tool Sik Chun (Joey) Lam, Derek Sleeman, and Wamberto in an established taxonomy; it suggests a number of ways in which that can be achieved namely; modifying the new entity, the taxonomy, or both. Further, a set of refinement operators are used to guarantee

Vasconcelos, Wamberto

118

Intergeneric somatic hybridization in Gramineae: somatic hybrid plants between tall fescue ( Festuca arundinacea Schreb.) and Italian ryegrass ( Lolium multiflorum Lam.)  

Microsoft Academic Search

Tall fescue (Festuca arundinacea Schreb.) protoplasts, inactivated by iodoacetamide, and non-morphogenic Italian ryegrass (Lolium multiflorum Lam.) protoplasts, both derived from suspension cultures, were electrofused and putative somatic hybrid plants were recovered. Two different genotypic fusion combinations were carried out and several green plants were regenerated in one of them. With respect to plant habitus, leaf and inflorescence morphology, the regenerants

Tadashi Takamizo; German Spangenberg; Ken-ichi Suginobu; Ingo Potrykus

1991-01-01

119

Mining MultiDimensional Constrained Gradients in Data Cubes Guozhu Dong Jiawei Han Joyce Lam Jian Pei Ke Wang  

E-print Network

Mining Multi­Dimensional Constrained Gradients in Data Cubes #3; Guozhu Dong Jiawei Han Joyce Lam interesting applications may need to analyze the changes of sophisticated measures in multidimensional space and describe sectors of the business modeled by the data cube. The problem of mining changes of sophisticated

Dong, Guozhu

120

An Improved Method of Isolation of High Quality Total RNA from Purple-Fleshed Sweet Potato, Ipomoea batatas (L.) Lam  

Microsoft Academic Search

To isolate high quality total RNA from plants of purple-fleshed sweet potato (Ipomoea batatas L. Lam) is difficult because these plants contain high levels of carbohydrates and pigment compounds. In this study, we developed a quick and reliable method for total RNA extraction. Using this extraction protocol, which was derived from the traditional CTAB method with modification, we obtained high

Wei Zhou; Yifu Gong; Qili Feng; Feng Gao

2009-01-01

121

Environmental Hydraulics and Sustainable Water Management Lee & Lam (eds) 2005 Taylor & Francis Group, London, ISBN 04 1536 546 5  

E-print Network

Environmental Hydraulics and Sustainable Water Management ­ Lee & Lam (eds) © 2005 Taylor & Francis Group, London, ISBN 04 1536 546 5 Preliminary numerical study on groundwater system at the Hong Kong groundwater flow. The aim of this study is to understand the abnormally high pore water pressure in the basal

Jiao, Jiu Jimmy

122

MicroRNA-21 is Induced by Rapamycin in a Model of Tuberous Sclerosis (TSC) and Lymphangioleiomyomatosis (LAM)  

PubMed Central

Lymphangioleiomyomatosis (LAM), a multisystem disease of women, is manifest by the proliferation of smooth muscle-like cells in the lung resulting in cystic lung destruction. Women with LAM can also develop renal angiomyolipomas. LAM is caused by mutations in the tuberous sclerosis complex genes (TSC1 or TSC2), resulting in hyperactive mammalian Target of Rapamycin (mTOR) signaling. The mTOR inhibitor, Rapamycin, stabilizes lung function in LAM and decreases the volume of renal angiomyolipomas, but lung function declines and angiomyolipomas regrow when treatment is discontinued, suggesting that factors induced by mTORC1 inhibition may promote the survival of TSC2-deficient cells. Whether microRNA (miRNA, miR) signaling is involved in the response of LAM to mTORC1 inhibition is unknown. We identified Rapamycin-dependent miRNA in LAM patient angiomyolipoma-derived cells using two separate screens. First, we assayed 132 miRNA of known significance to tumor biology. Using a cut-off of >1.5-fold change, 48 microRNA were Rapamycin-induced, while 4 miRs were downregulated. In a second screen encompassing 946 miRNA, 18 miRs were upregulated by Rapamycin, while eight were downregulated. Dysregulation of miRs 29b, 21, 24, 221, 106a and 199a were common to both platforms and were classified as candidate “RapamiRs.” Validation by qRT-PCR confirmed that these microRNA were increased. miR-21, a pro-survival miR, was the most significantly increased by mTOR-inhibition (p<0.01). The regulation of miR-21 by Rapamycin is cell type independent. mTOR inhibition promotes the processing of the miR-21 transcript (pri-miR-21) to a premature form (pre-miR-21). In conclusion, our findings demonstrate that Rapamycin upregulates multiple miRs, including pro-survival miRs, in TSC2-deficient patient-derived cells. The induction of miRs may contribute to the response of LAM and TSC patients to Rapamycin therapy. PMID:23555865

Neuman, Nicole A.; Myachina, Faina; Yu, Jane; Priolo, Carmen; Henske, Elizabeth P.

2013-01-01

123

Chemical and sensory comparison of fresh and dried lulo (Solanum quitoense Lam.) fruit aroma.  

PubMed

The odour-active volatile compounds of lulo fruit (Solanum quitoense Lam.) were isolated by solvent extraction followed by solvent-assisted flavour evaporation (SAFE). GC-O and GCMS analyses as well as quantitation by internal standard method showed that (Z)-3-hexenal, ethyl butanoate, 3-sulphanylhexyl acetate, and ethyl hexanoate were key aroma compounds in this fruit. Other odorants with relevance because their contribution (high OAVs) to the overall aroma were 4-hydroxy-2,5-dimethyl-3(2H)-furanone, methyl benzoate, (E)-2-hexenal, and hexanal. Lulo fruit pulp in presence of maltodextrin DE-20 was dried by using four different types of drying methods: hot air-drying (HD), spray drying (SD), lyophilisation (LD), and ultrasonic convective hot air-drying (HUD). LD sample exhibited the highest sensory rank (lulo-like) in comparison with fresh fruit pulp. Hot-air drying processes (HD and HUD) changed adversely the aroma of lulo fruit pulp. PMID:25236202

Forero, Diana Paola; Orrego, Carlos Eduardo; Peterson, Devin Grant; Osorio, Coralia

2015-02-15

124

Effect of Moringa oleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats.  

PubMed

In India, drumstick (Moringa oleifera Lam. (Moringaceae)) is commonly used as a phytotherapeutic agent. The effect of oral administration of aqueous and alcoholic extract of Moringa oleifera root-wood on calcium oxalate urolithiasis has been studied in male Wistar albino rats. Ethylene glycol feeding resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. Supplementation with aqueous and alcoholic extract of Moringa oleifera root-wood significantly reduced the elevated urinary oxalate, showing a regulatory action on endogenous oxalate synthesis. The increased deposition of stone forming constituents in the kidneys of calculogenic rats was also significantly lowered by curative and preventive treatment using aqueous and alcoholic extracts. The results indicate that the root-wood of Moringa oleifera is endowed with antiurolithiatic activity. PMID:16386862

Karadi, Ravindra V; Gadge, Navneet B; Alagawadi, K R; Savadi, Rudraprabhu V

2006-04-21

125

Isolation and characterization of a flocculating protein from Moringa oleifera Lam.  

PubMed

A flocculating protein from the seeds of Moringa oleifera Lam. was isolated by extraction with phosphate buffer followed by cation exchange chromatography. The molecular mass of the protein determined by SDS-PAGE was about 6.5 kDa, the isoelectric point was above pH 10. Amino acid analysis and sequencing showed high contents of glutamine, arginine and proline, and a total of 60 residues. The amino terminus is blocked by pyroglutamate. The flocculant capacity, determined in glass powder suspension, is comparable to that of a cationic polymer on polyacrylamide basis. Flocculation activity may be explained by the patch charge mechanism due to low molecular weight and high charge density. PMID:7727523

Gassenschmidt, U; Jany, K D; Tauscher, B; Niebergall, H

1995-04-13

126

Extraction and chemical investigation of Kulthi (Macrotylona uniflorus, Lam.) seed protein.  

PubMed

Studies have been carried out on the protein solubility profile of Kulthi (Macrotylona uniflorus, Lam.) seed in aqueous solution over various pHs and at different concentrations of NaCl, Na2SO3, CaCl2, and MgCl2 at pH 8.0. Amino acid analysis of isolated protein identified 17 amino acids, 9 of which are essential. Gel-permeation chromatography on Sephadex G-200 revealed the presence of seven components in the protein fraction. Their molecular weights were determined by two comparable standard methods. Extractable Kulthi seed proteins in salt solutions were separated electrophoretically into eight fractions whose molecular weights were found to be 186,200, 131,800, 108,400, 91,200, 53,700, 44,700, 38,000, and 27,500. PMID:7847899

Basak, B; Bhattacharyya, U K; Sinhababu, A; Laskar, S

1994-12-01

127

Sweet potato (Ipomoea batatas [L.] Lam)--a valuable medicinal food: a review.  

PubMed

Ipomoea batatas (L.) Lam, also known as sweet potato, is an extremely versatile and delicious vegetable that possesses high nutritional value. It is also a valuable medicinal plant having anti-cancer, antidiabetic, and anti-inflammatory activities. Sweet potato is now considered a valuable source of unique natural products, including some that can be used in the development of medicines against various diseases and in making industrial products. The overall objective of this review is to give a bird's-eye view of the nutritional value, health benefits, phytochemical composition, and medicinal properties of sweet potato. Specifically, this review outlines the biological activities of some of the sweet potato compounds that have been isolated, the pharmacological action of the sweet potato extract, clinical studies, and plausible medicinal applications of sweet potato (along with a safety evaluation), and demonstrates the potential of sweet potato as a medicinal food. PMID:24921903

Mohanraj, Remya; Sivasankar, Subha

2014-07-01

128

Predicting weed migration from soil and climate maps. [Centaurea maculosa Lam  

SciTech Connect

Soil characteristics, elevation, annual precipitation, potential evapotranspiration, length of frost-free season, and mean maximum July temperature were estimated for 116 established infestations of spotted knapweed (Centaurea maculosa Lam. number/sup 3/ CENMA) in Montana using basic land resource maps. Areas potentially vulnerable to invasion by the plant were delineated on the basis of representative edaphic and climatic characteristics. No single environmental variable was an effective predictor of sites vulnerable to invasion by spotted knapweed. Only a combination of variables was effective, indicating that the factors that regulate adaptability of this plant are complex. This technique provides a first approximation map of the regions most similar environmentally to infested sites and; therefore, most vulnerable to further invasion. This weed migration prediction technique shows promise for predicting suitable habitats of other invader species. 6 references, 4 figures, 1 table.

Chicoine, T.K.; Fay, P.K.; Nielsen, G.A.

1985-01-01

129

Diversity of mollusks in the Lam Ta Khong reservoir, Nakhon Ratchasima, Thailand.  

PubMed

Sampling surveys to study the diversity of mollusks in the Lam Ta Khong reservoir, Nakhon Ratchasima Province, northeast Thailand, were carried out in the hot, rainy and cold seasons. The study area was divided into: Area I--the upper part where the Lam Ta Khong river drains; Area II--the mid-section of the reservoir; Area III--behind the dam. Mollusks were collected from four locations on each bank (to the right and left) of each area. Each location was sampled to include 6 cross-sectional stations; in total, 144 stations were sampled. In the deep water, an Ekman dredge was used to collect samples; the scoop or manual method was used at the water's edge. Ten species of snails and four species of clams were found. The dominant species of snails were: Clea helena, Bithynia siamensis goniomphalos and Melanoides tuberculata; clams were dominated by Corbicula sp. The intermediate host of the human blood fluke was not found. The population of most mollusk species increased during the cold season while that of clams and that of some species of snails increased during the rainy season. Clams and operculate snails predominated in Areas II and III. Pulmonate snails were mostly found close to the bank and on aquatic plants especially in Areas I and II. Operculate snails and clams mainly inhabited water 1 to 10 m deep. Two species of edible mollusks were found: Filopaludina martensi martensi and large numbers of Corbicula. Neither shedding light nor digestion with pepsin A revealed any human parasites in the mollusks sampled. PMID:12757219

Tesana, Smarn

2002-12-01

130

Pre and post-implantation changes in the uteus of rats: response to moringa oleifera lam. Extract.  

PubMed

Aqueous extract of Moringa oleifera Lam. (root) has been studies on pre and post-implementation stages of the uterus of rats so as to elucidate its antifertility mode of action. Results on the biochemical estimation in the uterus of control pregnant rats at different stages of pregnancy revealed a successive increase in the total proteins, glycogen content and the activity or acid and alkaline phosphatase from day 2 to 5 post-coitum. When aqueous extract of M. oleifera Lam. Was administered, there was a significant reduction in all these biochemical constituents when compared to their respective control groups. The role of these biochemical transformations has been discussed in relation to anti-implantation action of the extract. PMID:22557630

Prakash, A O; Pathak, S; Shukla, S; Mathur, R

1988-07-01

131

Chemical composition, antioxidant and antimicrobial properties of the essential oil of Dacryodes edulis (G. Don) H. J. Lam from Gabon  

Microsoft Academic Search

The essential oil obtained by hydrodistillation from the resin of Dacryodes edulis (G. Don) H. J. Lam was simultaneously analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Twenty four components were identified in the essential oil and the main components were sabinene (21.8%), terpinene-4-ol (19.8%), ? ? ? ?-pinene (17.5%) and p-cymene (11.3%), respectively. The antioxidant capacity of

L. C. Obame; P. Edou; I. H. N. Bassolé; J. Koudou; H. Agnaniet; F. Eba

132

Long-range sources of Toronto particulate matter (PM 2.5) identified by Aerosol Laser Ablation Mass Spectrometry (LAMS)  

Microsoft Academic Search

An aerosol laser ablation mass spectrometer (LAMS) was operated continuously in downtown Toronto for a period of 11 months. The mass spectra of individual particles were classified according to their chemical composition using neural net-based adaptive resonance theory (ART-2a). The types of particles thereby identified were used as the basis for classifying particles from a 10-day sampling period within the

Sandy Owega; Greg J. Evans; Robert E. Jervis; Mike Fila; Ryan D’Souza; Badi-Uz-Zaman Khan

2004-01-01

133

EFECTO PERJUDICIAL DE Moringa oleifera (Lam.) COMBINADA CON OTROS DESECHOS AGRÍCOLAS COMO SUSTRATOS PARA LA LOMBRIZ ROJA (Eisenia spp.)  

Microsoft Academic Search

SUMMARY An experiment was carried out to study the behavior of the red worm (Eisenia spp.) fed with 5 nutritious substrates based in Moringa oleifera (Lam.) foliage, for the nutritional potentiali- ties, and other agricultural wastes in Trujillo state, Venezuela. A randomized design with repeated measurements and three repli- cates was used. Initially, 1.16kg of biomass in 0.5m3 of substrate

Luis José Cov; Danny Eugenio García; Alexander Rafael Castro; María Gabriela Medina

2007-01-01

134

Increased resistance to crown rust disease in transgenic Italian ryegrass ( Lolium multiflorum Lam.) expressing the rice chitinase gene  

Microsoft Academic Search

We introduced the rice chitinase (Cht-2; RCC2) gene into calli of Italian ryegrass (Lolium multiflorum Lam.), with a hygromycin phosphotransferase (HPT) gene as a selectable marker, by particle bombardment. Hygromycin-resistant calli were selected and transferred to regeneration medium for shoot formation. Polymerase chain reaction (PCR) analysis revealed regenerants containing the HPT gene. The RCC2 gene was detected in 65.5% of

Wataru Takahashi; Masahiro Fujimori; Yuichi Miura; Toshinori Komatsu; Yoko Nishizawa; Tadaaki Hibi; Tadashi Takamizo

2005-01-01

135

Trace element partition coefficients for clinopyroxene and phlogopite in an alkaline lamprophyre from Newfoundland by LAM-ICP-MS  

Microsoft Academic Search

Mineral\\/rock matrix partition coefficients have been determined for clinopyroxene (Cpx) and phlogopite from a Mesozoic alkaline lamprophyre from Newfoundland, Canada, by Laser Ablation Microprobe (LAM-ICP-MS). Values for twenty-one elements were obtained for Cpx, whereas only eleven were possible for phlogopite due to REE abundances below detection limits ( 1 ppm). Ablation pits of 40–50 ?m diameter enabled investigation of zonation

Stephen F. Foley; Simon E. Jackson; Brian J. Fryer; John D. Greenouch; George A. Jenner

1996-01-01

136

Trace element partition coefficients for clinopyroxene and phlogopite in an alkaline lamprophyre from Newfoundland by LAM-ICP-MS  

Microsoft Academic Search

Mineral\\/rock matrix partition coefficients have been determined for clinopyroxene (Cpx) and phlogopite from a Mesozoic alkaline lamprophyre from Newfoundland, Canada, by Laser Ablation Microprobe (LAM-ICP-MS). Values for twenty-one elements were obtained for Cpx, whereas only eleven were possible for phlogopite due to REE abundances below detection limits ( 1 ppm). Ablation pits of 40 50 mum diameter enabled investigation of

Stephen F. Foley; Simon E. Jackson; Brian J. Fryer; John D. Greenouch; George A. Jenner

1996-01-01

137

Bioactive Neo-Clerodane Diterpenoids Toward Tenebrio Molitor Larvae from Teucrium nudicaule H. and Baccharis spicata (Lam.) Beill  

Microsoft Academic Search

From the aerial parts of Teucrium nudicaule H. the previously known diterpenoids 6-acetilteucjaponin B and triacetylteumassilin have been isolated together with the new C-12 epimer of teupyreinin as well as the flavones cirsiliol and eupatorin. From Baccharis spicata (Lam) Beill., the neo-clerodane bacchotricuneatin A and 6?-hydroxybacchotricuneatin A were isolated. Some of these compounds have been subjected to a series of

O. V. Gallardo; C. E. Tonn; M. Nieto; G. B. Morales; O. S. Giordano

1996-01-01

138

Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats  

Microsoft Academic Search

The leaves of Moringa oleifera Lam (Moringaceae) are used by the Indians in their herbal medicine as a hypocholesterolemic agent in obese patients. The scientific basis for their use in hypercholesterolemia was therefore examined. It was found that administration of the crude leaf extract of Moringa oleifera along with high-fat diet decreased the high-fat diet-induced increases in serum, liver, and

S Ghasi; E Nwobodo; J. O Ofili

2000-01-01

139

Chemomodulatory Effect of Moringa Oleifera, Lam, on Hepatic Carcinogen Metabolising Enzymes, Antioxidant Parameters and Skin Papillomagenesis in Mice  

Microsoft Academic Search

The modulatory effects of a hydro-alchoholic extract of drumsticks of Moringa oliefera Lam at doses of 125 mg\\/ kg bodyweight and 250 mg\\/ kg body weight for 7 and 14 days, respectively, were investigated with reference to drug metabolising Phase I (Cytochrome b 5 and Cytochrome P 450 ) and Phase II (Glutathione-S- transferase) enzymes, anti-oxidant enzymes, glutathione content and

Rupjyoti Bharali; Jawahira Tabassum; Mohammed Rekibul; Haque Azad

2003-01-01

140

Nutrient Composition of Selected Sweet Potato [Ipomea batatas (L) Lam] Varieties as Influenced by Different Levels of Nitrogen Fertilizer Application  

Microsoft Academic Search

2 Abstract: Total ß-carotene content of four varieties of Sweet potatoes (Ipomoea batatas (L) Lam) a s influenced by different levels of nitrogen fertilizer were determined by High Performance Liquid Chromatography (HPLC) The proximate composition and mineral contents were also determined. The nitrogen fertilizer treatments were combinations of four levels of 0kgN\\/ha(control), 40 kgN\\/ha, 80 kg\\/Nha, 120 kgN\\/ha on the

A. N. Ukom; P. C. Ojimelukwe; D. A. Okpara

2009-01-01

141

A valued Indian medicinal plant - Begonia malabarica Lam. : Successful plant regeneration through various explants and field performance  

Microsoft Academic Search

A cost-effective and efficient protocol has been described in the present work for large- scale and rapid in vitro propagation of a valuable medicinal herb Begonia malabarica Lam. (Begoniaceae) by shoot auxillary-bud proliferation and organogenesis on MS medium supplemented with 6-benzylaminopurine (BA; 0.0-8.8 mg\\/l) and indole-3-acetic acid (IAA; 0.0-2.88 mg\\/l) at different concentrations, either alone or in combinations. Initiation of

Mathan C. Nisha; Sevanan Rajeshkumar; Thangavel Selvaraj

142

Uterine histoarchitecture during pre and post-implantation periods of rats treated with aqueous extract of Moringa oleifera Lam.  

PubMed

The effect of aqueous extract of Moringa oleifera Lam. (roots) has been studied on histoarchitecture of the uterus during pre and post-implantation stages in rats so as to elucidate its antifertility mode of action. The histoarchitecture of the uterus of control pregnant rat had revealed a clear-cut close apposition of the uterine endometrium with reduced lumen and loose stroma. There was a prominent appearance of deciduoma and the uterine glands were enlarged. Glandular cells showed hypertrophy and in the endometrium the leucocytic infiltration was increased. When the aqueous extract of M. oleifera Lam. was administered, no deciduoma was observed on day 5th of pregnancy and the luminal epithelium remained unstimulated. The lumen was enlarged and the uterus was non-oedematous. It has been concluded that the administration of aqueous extract of M. oleifera Lam. to pregnant rats could not stimulate the uterus which remained non-receptive throughout the period of treatment, therefore, the fertilized eggs may not be welcomed by the unprepared uterus. PMID:3630576

Prakash, A O; Pathak, S; Shukla, S; Mathur, R

1987-01-01

143

The C-terminal portion of the tail fiber protein of bacteriophage lambda is responsible for binding to LamB, its receptor at the surface of Escherichia coli K-12.  

PubMed

Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residues of J could bind to LamB trimers and inhibited recognition by anti-LamB antibodies. Electron microscopy further demonstrated that the fusion protein could also bind to LamB at the surface of intact cells. This interaction prevented lambda adsorption but affected only partially maltose uptake. PMID:10629200

Wang, J; Hofnung, M; Charbit, A

2000-01-01

144

The C-Terminal Portion of the Tail Fiber Protein of Bacteriophage Lambda Is Responsible for Binding to LamB, Its Receptor at the Surface of Escherichia coli K-12  

PubMed Central

Bacteriophage ? adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of ?, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residues of J could bind to LamB trimers and inhibited recognition by anti-LamB antibodies. Electron microscopy further demonstrated that the fusion protein could also bind to LamB at the surface of intact cells. This interaction prevented ? adsorption but affected only partially maltose uptake. PMID:10629200

Wang, Jiang; Hofnung, Maurice; Charbit, Alain

2000-01-01

145

A role for residue 151 of LamB in bacteriophage lambda adsorption: possible steric effect of amino acid substitutions.  

PubMed Central

LamB is the cell surface receptor for bacteriophage lambda. LamB missense mutations yielding resistance to lambda have been previously grouped in two classes. Class I mutants block growth of lambda with wild-type host range (lambda h+) but support growth of one-step extended-host-range mutants (lambda h). Class II mutants block lambda h but support growth of two-step extended host range mutants (lambda hh*). While Class I mutations occur at 11 different amino acid sites, in five distinct portions of LamB, all the Class II mutations analyzed previously correspond to the same G-to-D change at amino acid 151. We generated by in vitro mutagenesis four different new substitutions at site 151 (to S, V, R, and C). Two of the mutants (G-151-->V [G151V] and G151R) were of Class II, while the two others (G151S and G151C) were of Class I, demonstrating that not only the site but also the nature of the substitutions at residue 151 was critical for the phage sensitivity phenotypes. The introduction of a negatively charged, a positively charged, or an aliphatic nonpolar residue at site 151 of LamB prevented both lambda h+ and lambda h adsorption, indicating that the block is not due to a charge effect. In contrast to G151D, which was sensitive to all the lambda hh* phages, G151V and G151R conferred sensitivity to only four of the five lambda hh* phages. Thus, G151V and G151R represent a new subclass of Class II LamB mutations that is more restrictive with respect to the growth of lambda hh*. Our results agree with the hypothesis that residue 151 belongs to an accessibility gate controlling the access to the phage tight-binding site and that substitutions at this residue affect the access of the phage to the binding site in relation to the size of the substitute side chain (surface area): the most restrictive changes are G151V and G151R, followed to a lesser extent by G151D and they by G151S and G151C. Images PMID:8195074

Charbit, A; Werts, C; Michel, V; Klebba, P E; Quillardet, P; Hofnung, M

1994-01-01

146

A role for residue 151 of LamB in bacteriophage lambda adsorption: possible steric effect of amino acid substitutions.  

PubMed

LamB is the cell surface receptor for bacteriophage lambda. LamB missense mutations yielding resistance to lambda have been previously grouped in two classes. Class I mutants block growth of lambda with wild-type host range (lambda h+) but support growth of one-step extended-host-range mutants (lambda h). Class II mutants block lambda h but support growth of two-step extended host range mutants (lambda hh*). While Class I mutations occur at 11 different amino acid sites, in five distinct portions of LamB, all the Class II mutations analyzed previously correspond to the same G-to-D change at amino acid 151. We generated by in vitro mutagenesis four different new substitutions at site 151 (to S, V, R, and C). Two of the mutants (G-151-->V [G151V] and G151R) were of Class II, while the two others (G151S and G151C) were of Class I, demonstrating that not only the site but also the nature of the substitutions at residue 151 was critical for the phage sensitivity phenotypes. The introduction of a negatively charged, a positively charged, or an aliphatic nonpolar residue at site 151 of LamB prevented both lambda h+ and lambda h adsorption, indicating that the block is not due to a charge effect. In contrast to G151D, which was sensitive to all the lambda hh* phages, G151V and G151R conferred sensitivity to only four of the five lambda hh* phages. Thus, G151V and G151R represent a new subclass of Class II LamB mutations that is more restrictive with respect to the growth of lambda hh*. Our results agree with the hypothesis that residue 151 belongs to an accessibility gate controlling the access to the phage tight-binding site and that substitutions at this residue affect the access of the phage to the binding site in relation to the size of the substitute side chain (surface area): the most restrictive changes are G151V and G151R, followed to a lesser extent by G151D and they by G151S and G151C. PMID:8195074

Charbit, A; Werts, C; Michel, V; Klebba, P E; Quillardet, P; Hofnung, M

1994-06-01

147

Achyrocline satureioides (Lam.) D.C. Hydroalcoholic Extract Inhibits Neutrophil Functions Related to Innate Host Defense  

PubMed Central

Achyrocline satureioides (Lam.) D.C. is a herb native to South America, and its inflorescences are popularly employed to treat inflammatory diseases. Here, the effects of the in vivo actions of the hydroalcoholic extract obtained from inflorescences of A. satureioides on neutrophil trafficking into inflamed tissue were investigated. Male Wistar rats were orally treated with A. satureioides extract, and inflammation was induced one hour later by lipopolysaccharide injection into the subcutaneous tissue. The number of leukocytes and the amount of chemotactic mediators were quantified in the inflammatory exudate, and adhesion molecule and toll-like receptor 4 (TLR-4) expressions and phorbol-myristate-acetate- (PMA-) stimulated oxidative burst were quantified in circulating neutrophils. Leukocyte-endothelial interactions were quantified in the mesentery tissue. Enzymes and tissue morphology of the liver and kidney were evaluated. Treatment with A. satureioides extract reduced neutrophil influx and secretion of leukotriene B4 and CINC-1 in the exudates, the number of rolling and adhered leukocytes in the mesentery postcapillary venules, neutrophil L-selectin, ?2-integrin and TLR-4 expression, and oxidative burst, but did not cause an alteration in the morphology and activities of liver and kidney. Together, the data show that A. satureioides extract inhibits neutrophil functions related to the innate response and does not cause systemic toxicity. PMID:23476704

Barioni, Eric Diego; Machado, Isabel Daufenback; Rodrigues, Stephen Fernandes de Paula; Ferraz-de-Paula, Viviane; Wagner, Theodoro Marcel; Cogliati, Bruno; Correa dos Santos, Matheus; Machado, Marina da Silva; de Andrade, Sergio Faloni; Niero, Rivaldo; Farsky, Sandra Helena Poliselli

2013-01-01

148

Gene Expression Rhythms in the Mussel Mytilus galloprovincialis (Lam.) across an Annual Cycle  

PubMed Central

Seasonal environmental changes may affect the physiology of Mytilus galloprovincialis (Lam.), an intertidal filter-feeder bivalve occurring commonly in Mediterranean and Atlantic coastal areas. We investigated seasonal variations in relative transcript abundance of the digestive gland and the mantle (gonads) of males and females. To identify gene expression trends – in terms of relative mRNA abundance- we used a medium-density cDNA microarray (1.7 K probes) in dual-color competitive hybridization analyses. Hierarchical clustering of digestive gland microarray data showed two main branches, distinguishing profiles associated with the “hot” months (May–August) from the other months. Genes involved in chitin metabolism, associated with mussel nutrition and digestion showed higher mRNA levels during summer. Moreover, we found different gene transcriptomic patterns in the digestive glands of males when compared to females, during the four stages of mussel gonadal development. Microarray data from gonadal transcripts also displayed clear patterns during the different developmental phases respect to the resting period (stage I) with peak relative mRNA abundance at the ripe phase (stage III) for both sexes. These data showed a clear temporal pattern in transcriptomic profiles of mussels sampled over an annual cycle. Physiological response to thermal variation, food availability, and reproductive status across months may contribute to variation in relative mRNA abundance. PMID:21573210

Banni, Mohamed; Negri, Alessandro; Mignone, Flavio; Boussetta, Hamadi; Viarengo, Aldo; Dondero, Francesco

2011-01-01

149

Removal of cadmium from aqueous system by shelled Moringa oleifera Lam. seed powder.  

PubMed

The present study explores the unexploited sorption properties of the plant Moringa oleifera Lam. for decontamination of Cd at laboratory scale. Sorption studies using standard practices were carried out in batch experiments as functions of biomass dosage, contact time, metal concentrations, particle size and pH. Percentage sorption in each case was computed on the basis of Cd estimation using a planar NaI (TI) detector coupled to a 4K MCA (Canberra Accuspec Card with PC-AT 386). The adsorption data accurately in a Freundlich isotherm. Sorption studies resulted in the standardization of optimum conditions for removal of Cd (85.10%) as follows: biomass dosage (4.0 g), metal concentration (25 microg/ml), contact time (40 min) and volume of the test solution (200 ml) at pH 6.5. Fourier transform infrared (FTIR) spectrometry highlighted amino acid-Cd interactions responsible for sorption phenomenon. The findings open up new avenues in the removal of toxic metals by shelled Moringa oleifera seeds (SMOS) from water bodies as low cost, domestic and environmentally friendly safe technology. PMID:15949938

Sharma, Parul; Kumari, Pushpa; Srivastava, M M; Srivastava, Shalini

2006-01-01

150

Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione level.  

PubMed

Initiation of acetaminophen (APAP) toxicities is believed to be promoted by oxidative stress during the event of overdosage. The aim of the present study was to evaluate the hepatoprotective action of Moringa oleifera Lam (MO), an Asian plant of high medicinal value, against a single high dose of APAP. Groups of five male Sprague-Dawley rats were pre-administered with MO (200 and 800 mg/kg) prior to a single dose of APAP (3g/kg body weight; p.o). Silymarin was used as an established hepatoprotective drug against APAP induced liver injury. The hepatoprotective activity of MO extract was observed following significant histopathological analysis and reduction of the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in groups pretreated with MO compared to those treated with APAP alone. Meanwhile, the level of glutathione (GSH) was found to be restored in MO-treated animals compared to the groups treated with APAP alone. These observations were comparable to the group pretreated with silymarin prior to APAP administration. Group that was treated with APAP alone exhibited high level of transaminases and ALP activities besides reduction in the GSH level. The histological hepatocellular deterioration was also evidenced. The results from the present study suggested that the leaves of MO can prevent hepatic injuries from APAP induced through preventing the decline of glutathione level. PMID:18514995

Fakurazi, S; Hairuszah, I; Nanthini, U

2008-08-01

151

In vitro hypoglycemic effects of Butea monosperma Lam. leaves and bark.  

PubMed

The present study evaluated the antidiabetic potential of Butea monosperma Lam. Kuntze (Fabaceae) leaves and the stem bark using various in vitro techniques. The samples were studied for their effects on glucose adsorption, diffusion, amylolysis kinetics, enteric enzymes and glucose transport across yeast cells. Both the samples adsorbed glucose and the adsorption of glucose increased with increase in glucose concentration. The samples also inhibited movement of glucose across the dialysis membrane to varying degree in both glucose diffusion and amylolysis kinetic experiment models. B. monosperma leaves inhibited ?-amylase, ?-glucosidase and sucrase enzymes in succession to varying degrees, whereas the bark inhibited only ?-amylase to a significant extent and slightly activated ?-glucosidase and sucrase enzymes. The extracts of both leaves and bark promoted glucose uptake by yeast cells compared to control. Enhancement of glucose uptake was dependent on both the sample and glucose concentration. It was directly proportional to the sample concentration and inversely proportional to the molar concentration of the glucose. From the results of the study, it is inferred that, B. monosperma leaves and bark possesses antidiabetic activity. However, these effects need to be confirmed using in vivo models and clinical trials for its effective utilization as therapeutic agents. PMID:24493888

Harish, M; Ahmed, Faiyaz; Urooj, Asna

2014-02-01

152

Hybridization and invasion: an experimental test with diffuse knapweed (Centaurea diffusa Lam.)  

PubMed Central

A number of studies have suggested a link between hybridization and invasion. In this study, we experimentally test the potential for hybridization to influence invasion through a greenhouse common garden study. Diffuse knapweed (DK) (Centaurea diffusa Lam.) was introduced to North America with admixture from spotted knapweed (SK) (Centaurea stoebe subsp. stoebe L.). Comparisons between North American DK (including hybrid phenotypes) and native (European) DK in a common garden did not reveal enhanced performance or increased phenotypic variance, suggesting that pre-introduction hybridization or, more generally, post-introduction evolutionary change has not significantly contributed to the invasion of DK. In contrast, early generation hybrids [artificially created Backcross 1 (BC1) plants] exhibited increased variance for eight of the examined traits, and greater leaf and reproductive shoot production when compared to North American DK. Individual BC1 lines differed for several traits, suggesting the importance of the cross for drawing conclusions from such comparisons. When compared to the parental species (DK and SK), the BC1 plants were not transgressive for any of the measured traits. Overall, these findings suggest that if diploid SK is introduced to North America, interspecific hybridization has the potential to result in even more aggressive invaders.

Blair, Amy C; Blumenthal, Dana; Hufbauer, Ruth A

2012-01-01

153

Disentangling the Origins of Cultivated Sweet Potato (Ipomoea batatas (L.) Lam.)  

PubMed Central

Sweet potato (Ipomoea batatas (L.) Lam., Convolvulaceae) counts among the most widely cultivated staple crops worldwide, yet the origins of its domestication remain unclear. This hexaploid species could have had either an autopolyploid origin, from the diploid I. trifida, or an allopolyploid origin, involving genomes of I. trifida and I. triloba. We generated molecular genetic data for a broad sample of cultivated sweet potatoes and its diploid and polyploid wild relatives, for noncoding chloroplast and nuclear ITS sequences, and nuclear SSRs. Our data did not support an allopolyploid origin for I. batatas, nor any contribution of I. triloba in the genome of domesticated sweet potato. I. trifida and I. batatas are closely related although they do not share haplotypes. Our data support an autopolyploid origin of sweet potato from the ancestor it shares with I. trifida, which might be similar to currently observed tetraploid wild Ipomoea accessions. Two I. batatas chloroplast lineages were identified. They show more divergence with each other than either does with I. trifida. We thus propose that cultivated I. batatas have multiple origins, and evolved from at least two distinct autopolyploidization events in polymorphic wild populations of a single progenitor species. Secondary contact between sweet potatoes domesticated in Central America and in South America, from differentiated wild I. batatas populations, would have led to the introgression of chloroplast haplotypes of each lineage into nuclear backgrounds of the other, and to a reduced divergence between nuclear gene pools as compared with chloroplast haplotypes. PMID:23723970

Roullier, Caroline; Duputie, Anne; Wennekes, Paul; Benoit, Laure; Fernandez Bringas, Victor Manuel; Rossel, Genoveva; Tay, David; McKey, Doyle; Lebot, Vincent

2013-01-01

154

Drell-Yan production and Lam-Tung relation in the Color Glass Condensate formalism  

E-print Network

We study the Drell-Yan production cross section and structure functions in proton (deuteron)-nucleus collisions using the Color Glass Condensate formalism. The nucleus is treated in the Color Glass Condensate framework which includes both higher twist effects due to the inclusion of multiple scatterings and leading twist pQCD shadowing due to the small x resummation, while the proton (or deuteron) is treated within the DGLAP improved parton model. In particular, the Drell-Yan structure functions are used in order to investigate the Lam-Tung relation at small x, which is known to be identically zero at leading twist up to Next-to-Leading order, and is thus a good playground for studying higher twist effects. In agreement with this, we find that violations of this relation are more important for low momentum and invariant mass of the Drell-Yan pair, and also in the region of rapidity that corresponds to smaller values of x in the nucleus.

Francois Gelis; Jamal Jalilian-Marian

2006-09-06

155

Hexane soluble extract of Mallotus philippensis (Lam.) Muell. Arg. root possesses anti-leukaemic activity  

PubMed Central

Background Mallotus philippensis (Lam.) Muell. Arg. is a well known medicinal plant of Asia and Australia. Various compounds from different aerial parts of the plant have been reported possessing potent pharmacological, antiviral, antibacterial and cytotoxic activities. We were interested to determine the effects of some root extracts from M. philippensis on human promyelocytic leukemia HL-60 cell proliferation, cell cycle regulators and apoptosis in order to investigate its anti-leukemic potential. Results Root extract of M. philippensis was initially extracted in organic solvents, hexane, ethyl acetate, and n-butanol. The hexane extract showed highest toxicity against p53-deficient HL-60 cells (IC50 1.5 mg dry roots equivalent/ml medium) after 72 h and interestingly, inhibition of cell proliferation was preceded by the upregulation of the proto-oncogenes Cdc25A and cyclin D1 within 24 h. The hexane extract induced 18% apoptosis after 48 h of treatment. Chemical composition of the hexane extract was analyzed by GC-MS and the 90% fragments were matched with polyphenolic compounds. Conclusions The present study confirms that the hexane fraction of M. philippensis root extract possesses anti-leukemic activity in HL-60 cells. The polyphenols were the main compounds of the hexane extract that inhibited proliferation and induced apoptosis. PMID:24041220

2013-01-01

156

Investigation on the influence of foreign metal ions in crystal growth and characterization of L-Alaninium Maleate (LAM) single crystals  

NASA Astrophysics Data System (ADS)

A Nonlinear Optical, good quality, single crystals of doped and undoped L-Alaninium Maleate (LAM) were grown by slow evaporation solution growth technique at room temperature. The lattice parameters were analyzed by single crystal X-ray diffraction technique. The identification of Cadmium ion in the doped crystals was done using the EDAX spectrum. The presence of functional group of the dopant with LAM molecule was studied using FTIR spectra. The results of UV-Vis study is used to compare the transparencies of the doped and undoped LAM crystals. The optical band gap energy of the grown crystal was also calculated. The relative second harmonic generation (SHG) efficiency measurement with KDP reference is used to find the incorporation of metal to L-Alaninium Maleate crystals and the parent material. Also the thermal stability of the grown crystals was studied by TGA/DTA spectrum. The mechanical stability of the grown crystals was confirmed through Vickers micro hardness study. By parallel plate capacitor technique, the dielectric response was studied over a wide range of frequencies at different temperatures. The various studies showed the incorporation of the impurity Cd2+ into LAM crystals and the investigations indicated that the impurity played an important role in the changes of the spectral and structural properties of LAM crystals.

Ruby Nirmala, L.; Thomas Joseph Prakash, J.

2013-11-01

157

Folding studies of purified LamB protein, the maltoporin from the Escherichia coli outer membrane: trimer dissociation can be separated from unfolding.  

PubMed

The folding mechanisms for ?-barrel membrane proteins present unique challenges because acquisition of both secondary and tertiary structure is coupled with insertion into the bilayer. For the porins in Escherichia coli outer membrane, the assembly pathway also includes association into homotrimers. We study the folding pathway for purified LamB protein in detergent and observe extreme hysteresis in unfolding and refolding, as indicated by the shift in intrinsic fluorescence. The strong hysteresis is not seen in unfolding and refolding a mutant LamB protein lacking the disulfide bond, as it unfolds at much lower denaturant concentrations than wild type LamB protein. The disulfide bond is proposed to stabilize the structure of LamB protein by clasping together the two sides of Loop 1 as it lines the inner cavity of the barrel. In addition we find that low pH promotes dissociation of the LamB trimer to folded monomers, which run at about one third the size of the native trimer during SDS PAGE and are much more resistant to trypsin than the unfolded protein. We postulate the loss at low pH of two salt bridges between Loop 2 of the neighboring subunit and the inner wall of the monomer barrel destabilizes the quaternary structure. PMID:21640073

Baldwin, Valerie; Bhatia, Mandeep; Luckey, Mary

2011-09-01

158

Investigation on the influence of foreign metal ions in crystal growth and characterization of L-Alaninium Maleate (LAM) single crystals.  

PubMed

A Nonlinear Optical, good quality, single crystals of doped and undoped l-Alaninium Maleate (LAM) were grown by slow evaporation solution growth technique at room temperature. The lattice parameters were analyzed by single crystal X-ray diffraction technique. The identification of Cadmium ion in the doped crystals was done using the EDAX spectrum. The presence of functional group of the dopant with LAM molecule was studied using FTIR spectra. The results of UV-Vis study is used to compare the transparencies of the doped and undoped LAM crystals. The optical band gap energy of the grown crystal was also calculated. The relative second harmonic generation (SHG) efficiency measurement with KDP reference is used to find the incorporation of metal to l-Alaninium Maleate crystals and the parent material. Also the thermal stability of the grown crystals was studied by TGA/DTA spectrum. The mechanical stability of the grown crystals was confirmed through Vickers micro hardness study. By parallel plate capacitor technique, the dielectric response was studied over a wide range of frequencies at different temperatures. The various studies showed the incorporation of the impurity Cd(2+) into LAM crystals and the investigations indicated that the impurity played an important role in the changes of the spectral and structural properties of LAM crystals. PMID:23892119

Ruby Nirmala, L; Thomas Joseph Prakash, J

2013-11-01

159

Effect of Moringa oleifera Lam. seed extract on ovalbumin-induced airway inflammation in guinea pigs.  

PubMed

To determine the therapeutic potential of herbal medicine Moringa oleifera Lam. family: Moringaceae in the control of allergic diseases, the efficacy of the ethanolic extract of the seeds of the plant (MOEE) against ovalbumin (OVA)-induced airway inflammation in guinea pigs was examined. During the experimental period, the test drugs (MOEE or dexamethasone) were administered by oral route prior to challenge with aerosolized 0.5% OVA. Bronchoconstriction tests were performed and respiratory parameters (i.e., tidal volume and respiratory rate) were measured. At the end of experiment, blood was collected from each animal to perform total and differential counts and serum was used for assay of IL-4, IL-6, and TNFalpha. Lung lavage fluid (BAL) was collected for estimation of cellular content and cytokine levels. Lung tissue histamine assays were performed using the homogenate of one lobe from each animal; a separate lobe and the trachea were subjected to histopathology to measure the degree of any airway inflammation. The results suggest that in OVA-sensitized control animals that did not receive either drug, tidal volume (V(t)) was decreased, respiration rate (f) was increased, and both the total and differential cell counts in blood and BAL fluid were increased significantly. MOEE-treatment of sensitized hosts resulted in improvement in all parameters except BAL TNFalpha and IL-4. Moreover, MOEE-treatment also showed protection against acetylcholine-induced broncho-constriction and airway inflammation which was confirmed by histological observations. The results of these studies confirm the traditional claim for the usefulness of this herb in the treatment of allergic disorders like asthma. PMID:18686107

Mahajan, Shailaja G; Mehta, Anita A

2008-08-01

160

Inhibitory Action of Ethanolic Extract of Seeds of Moringa oleifera Lam. On Systemic and Local Anaphylaxis.  

PubMed

The current study characterizes the mechanism by which the seed extract of Moringa oleifera Lam (Moringaceae) decreases the mast cell-mediated immediate type hypersensitivity reaction. The immediate type hypersensitivity reaction is involved in many allergic diseases such as asthma and allergic rhinitis. Moringa oleifera, a shrub widely used in the traditional medicine in India, has been reported to possess anti-cancer, hypotensive, anti-arthritic, and anti-inflammatory activities. In the present study, the effects of the ethanolic extract of seeds of Moringa oleifera (MOEE-herbal remedy) on systemic and local anaphylaxis were investigated. The potential anti-anaphylactic effect of MOEE was studied in a mouse model of Compound 48/80-induced systemic anaphylactic shock. Passive cutaneous anaphylaxis activated by anti IgE-antibody was also used to assess the effect of MOEE. In addition, rat peritoneal mast cells (RPMC) were used to investigate the effect of MOEE on histamine release induced by compound 48/80. When administered 1 hr before 48/80 injection, MOEE at doses of 0.001-1.000 g/kg completely inhibited the inducible induced anaphylactic shock. MOEE significantly inhibited passive cutaneous anaphylaxis activated by anti-IgE antibody at a dose of 1 g/kg. When MOEE extract was given as pretreatment at concentrations ranging 0.1-100 mg/ml, the histamine release from the mast cells that was induced by the 48/80 was reduced in a dose-dependent manner. These results suggest a potential role for MOEE as a source of anti-anaphylactic agents for use in allergic disorders. PMID:18958739

Mahajan, Shailaja G; Mehta, Anita A

2007-10-01

161

Effect of Crude Extract of Eugenia jambolana Lam. on Human Cytochrome P450 Enzymes.  

PubMed

The fruit of Eugenia jambolana Lam. is very popular for its anti-diabetic property. Previous studies on the crude extract of E.?jambolana (EJE) have successfully explored the scientific basis for some of its traditional medicinal uses. Considering its wide use and consumption as a seasonal fruit, the present study investigates the ability of E.?jambolana to interact with cytochrome P450 enzymes. The standardized EJE was incubated with pooled human liver microsomes to assess the CYP2C9-, CYP2D6-, and CYP3A4-mediated metabolism of diclofenac, dextromethorphan, and testosterone, respectively. The metabolites formed after the enzymatic reactions were quantified by high performance liquid chromatography. EJE showed differential effect on cytochrome P450 activities with an order of inhibitory potential as CYP2C9?>?CYP3A4?>?CYP2D6 having IC50 of 76.69, 359.02, and 493.05?µg/mL, respectively. The selectivity of EJE for CYP2C9 rather than CYP3A4 and CYP2D6 led to perform the enzyme kinetics to explicate the mechanism underlying the inhibition of CYP2C9-mediated diclofenac 4'-hydroxylation. EJE was notably potent in inhibiting the reaction in a non-competitive manner with Ki of 84.85?±?5.27?µg/mL. The results revealed the CYP2C9 inhibitory potential of EJE with lower Ki value suggesting that EJE should be examined for its potential pharmacokinetic and pharmacodynamic interactions when concomitantly administered with other drugs. Copyright © 2014 John Wiley & Sons, Ltd. PMID:24590863

Chinni, Santhivardhan; Dubala, Anil; Kosaraju, Jayasankar; Khatwal, Rizwan Basha; Satish Kumar, M N; Kannan, Elango

2014-11-01

162

Oxygen consumption rate and Na+/K+-ATPase activity in early developmental stages of the sea urchin Paracentrotus lividus Lam.  

NASA Astrophysics Data System (ADS)

Changes in oxygen consumption rate and Na+/K+-ATPase activity during early development were studied in the sea urchin Paracentrotus lividus Lam. The oxygen consumption rate increased from 0.12 ?mol O2 mg protein-1 h-1 in unfertilized eggs to 0.38 ?mol O2 mg protein-1 h-1 25 min after fertilization. Specific activity of the Na+/K+-ATPase was significantly stimulated after fertilization, ranging up to 1.07 ?mol Pi h-1 mg protein-1 in the late blastula stage and slightly lower values in the early and late pluteus stages.

Tomši?, Sanja; Stankovi?, Suzana; Lucu, ?edomil

2011-09-01

163

Target site mutation and reduced translocation are present in a glyphosate-resistant Lolium multiflorum Lam. biotype from Spain.  

PubMed

The resistance mechanism of a glyphosate-resistant Lolium multiflorum Lam. biotype collected in Córdoba (Southern Spain) was examined. Resistance Factor values at three different growth stages ranged between 4.77 and 4.91. At 96 hours after treatment (HAT) the S biotype had accumulated seven times more shikimic acid than the R biotype. There were significant differences in translocation of (14)C-glyphosate between biotypes, i.e. at 96 HAT, the R biotype accumulated in the treated leaf more than 70% of the absorbed herbicide, in comparison with 59.21% of the S biotype; the R biotype translocated only 14.79% of the absorbed (14)C-glyphosate to roots, while in the S population this value was 24.79%. Visualization of (14)C-glyphosate by phosphor imaging showed a reduced distribution in the R biotype compared with the S. Glyphosate metabolism was not involved in the resistance mechanism due to both biotypes showing similar values of glyphosate at 96 HAT. Comparison of the EPSPS gene sequences between biotypes indicated that the R biotype has a proline 182 to serine amino acid substitution. In short, the resistance mechanism of the L. multiflorum Lam. biotype is due to an impaired translocation of the herbicide and an altered target site. PMID:22771431

González-Torralva, Fidel; Gil-Humanes, Javier; Barro, Francisco; Brants, Ivo; De Prado, Rafael

2012-09-01

164

Performance Comparison of a Matrix Solver on a Heterogeneous Network Using Two Implementations of MPI: MPICH and LAM  

NASA Technical Reports Server (NTRS)

Two of the current and most popular implementations of the Message-Passing Standard, Message Passing Interface (MPI), were contrasted: MPICH by Argonne National Laboratory, and LAM by the Ohio Supercomputer Center at Ohio State University. A parallel skyline matrix solver was adapted to be run in a heterogeneous environment using MPI. The Message-Passing Interface Forum was held in May 1994 which lead to a specification of library functions that implement the message-passing model of parallel communication. LAM, which creates it's own environment, is more robust in a highly heterogeneous network. MPICH uses the environment native to the machine architecture. While neither of these free-ware implementations provides the performance of native message-passing or vendor's implementations, MPICH begins to approach that performance on the SP-2. The machines used in this study were: IBM RS6000, 3 Sun4, SGI, and the IBM SP-2. Each machine is unique and a few machines required specific modifications during the installation. When installed correctly, both implementations worked well with only minor problems.

Phillips, Jennifer K.

1995-01-01

165

SWEET POTATO (IPOMOEA BATATAS (L.) LAM.) TISSUE AS A BIOCATALYST IN A PARAFFIN\\/GRAPHITE BIOSENSOR FOR HYDRAZINE DETERMINATION IN BOILER FEED WATER  

Microsoft Academic Search

A biosensor based on paraffin\\/graphite modified with a sweet potato (Ipomoea batatas (L.) Lam.) tissue as the source of peroxidase was developed and used for determining hydrazine in boiler feed water. This enzyme in the presence of hydrogen peroxide catalyses the oxidation of hydroquinone to p-benzoquinone from which electrochemical reduction back to hydroquinone was obtained at a peak potential of

Iolanda Cruz Vieira; Karina Omuro Lupetti; Orlando Fatibello-Filho

2002-01-01

166

Mathematical modeling of PCB bioaccumulation in Perna viridis K.N. Yu a,b,*, P.K.S. Lam b,c  

E-print Network

for almost 20 years, PCB congeners and related polyhalo- genated hydrocarbons still pose a serious threatMathematical modeling of PCB bioaccumulation in Perna viridis K.N. Yu a,b,*, P.K.S. Lam b,c , C In the present work, we built a mathematical model of polychlorinated biphenyl (PCB) bioaccumulation in Perna

Yu, K.N.

167

AVALIAÇÃO DA QUALIDADE DAS SEMENTES DE Moringa oleifera LAM. DURANTE O ARMAZENAMENTO Evaluation of quality of the drumstick seeds during the storage  

Microsoft Academic Search

Drumstick (Moringa oleifera Lam., Moringaceae) is a perennial species originating from the indian northwest that adapts to both irrigated and rain fed crop conditions and it is not very demanding on soils and fertilizers. It is grown in the brazilian northeast, specially in Ceará State, due to its use in clarification of cloudy waters. In spite of its recognized medicinal

Antonio Marcos; Esmeraldo Bezerra; Sebastião Medeiros Filho; João Batista; Santiago Freitas; Elizita Maria Teófilo

168

Mechanism of Supported Membrane Disruption by Antimicrobial Peptide Protegrin-1 Kin Lok H. Lam,,# Yuji Ishitsuka,,# Yishan Cheng, Karen Chien, Alan J. Waring,|,  

E-print Network

of antimicrobial activity of PG-1 comes from its ability to selectively disrupt the cell membrane of microorMechanism of Supported Membrane Disruption by Antimicrobial Peptide Protegrin-1 Kin Lok H. Lam been suggested as an important step in the membrane disruption process induced by antimicrobial

Lee, Ka Yee C.

169

Microcontamination control in a Lam 4400 plasma etcher: 15X reduction in particle defect densities during SF6\\/He silicon trench etch  

Microsoft Academic Search

Particles from the Lam 4400 blocked the etching of nitride and silicon during the trench etch for semi-recessed LOCOS field oxidation. This trench etch resulted in poor mean time between cleans (MTBC) of less than 500 wafers, reduced availability of the system, wafer scrappage up to 2.5%, and masked KLA defect source analysis at subsequent steps. EDX analysis of the

Shantanu Bhagvat; Christine Cusack; Larry Anderson

1997-01-01

170

Simulation of a Multi-agent Protocol for Task Allocation in Cooperative Design Kwang Mong Sim, Simon Chi Keung Shiu, and Martin Bun Lam  

E-print Network

Simulation of a Multi-agent Protocol for Task Allocation in Cooperative Design Kwang Mong Sim, Simon Chi Keung Shiu, and Martin Bun Lam Department of Computing, Hong Kong Polytechnic University, Hung) 2774-0842 ABSTRACT To achieve high performance in agent-based cooperative design, the effective

Shiu, Chi Keung Simon

171

Determination of infochemicals and the phytochemical screening of the foliage and stem-bark of Senna siamea (lam.) in Yola, Adamawa State  

Microsoft Academic Search

Alkaloids, anthraquinonnes, cardiac glycosides, flavonoids, phlobatannin, polyphenols, saponins, steroids, tannins and terpenoids were ten groups of infochemicals studied in the foliage and stem-bark of Senna siamea (Lam.), a plantation species grown in Yola. The experimental design was the Randomized Complete Block Design (RCBD) with four replications. Results obtained from the experiments indicated that all the ten groups of infochemicals were

Ayuba Daniel Kwada; I. O. Tell

172

Perera Lam: An environmental justice assessment of the Mississippi River Industrial Corridor in Louisiana, U.S. APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 11(4): 681-697.  

E-print Network

Perera ­ Lam: An environmental justice assessment of the Mississippi River Industrial Corridor JUSTICE ASSESSMENT OF THE MISSISSIPPI RIVER INDUSTRIAL CORRIDOR IN LOUISIANA, U.S. USING A GIS to assess the status of environmental justice concerns in the Mississippi River Industrial Corridor

173

Composition of the essential oils in various organs at different developmental stages of Ammi visnaga (L.) Lam. from Tunisia.  

PubMed

The composition of the essential oils isolated by hydrodistillation from various organs at different development stages of Ammi visnaga (L.) Lam. growing in Tunisia was determined by GC/MS analysis. In particular, the oil profiles of the leaves, stems, flower buds, roots, umbels, and fruits have been examined during the whole life cycle. The oil from the flowering aerial parts was characterized by a high content of isoamyl 2-methylbutanoate. After flowering and during desiccation and fructification, the umbels and fruits expressed a high content of linalool. The oils, extracted from the roots collected in the vegetatif, buds floral, and floral stages, were rich in monoterpene aldehydes, oxygenated monoterpenes, and monoterpene hydrocarbons. The highest level of non-terpene hydrocarbons was found at the flower-bud stage, represented by 61.3% of nonane. Among the monoterpenes, sabinene (12.5%) and ?-pinene (8.5%) were identified in the flower buds. PMID:22083912

Sellami, Hela Kallel; Flamini, Guido; Cioni, Pier Luigi; Smiti, Samira

2011-11-01

174

Bioactive secondary metabolites from Nigrospora sp. LLGLM003, an endophytic fungus of the medicinal plant Moringa oleifera Lam.  

PubMed

An endophytic fungus was isolated from the root of the medicinal plant Moringa oleifera Lam. Based on analyzing the rDNA sequence, the fungus was identified as Nigrospora sp. This is the first report of the isolation of endophytic Nigrospora from M. oleifera. By bioassay-guided fractionation, four antifungal secondary metabolites were isolated from liquid cultures of the fungus Nigrospora sp. LLGLM003, and their chemical structures were determined to be griseofulvin (1), dechlorogriseofulvin (2), 8-dihydroramulosin (3) and mellein (4) on the basis of spectroscopic analyses. Compound 2, 3 and 4 were isolated from Nigrospora sp. for the first time. In vitro antifungal assay showed that griseofulvin displayed clear inhibition of the growth of 8 plant pathogenic fungi. Dechlorogriseofulvin and mellein exhibited only weak antifungal activities, whereas 8-dihydroramulosin displayed no antifungal activities. PMID:22806033

Zhao, J H; Zhang, Y L; Wang, L W; Wang, J Y; Zhang, C L

2012-05-01

175

Biochemical and physiological alterations in female reproductive organs of cyclic rats treated with aqueous extract of Moringa oleifera Lam.  

PubMed

Biochemical and physiological alterations have been observed in the genital tract of female cyclic rats treated with aqueous extract of M. oleifera Lam. Its administration caused a significant increase in the glycogen contents, protein concentration, activity of acid and alkaline phosphatase and the level of total cholesterol in all the organs at initial days of treatment. However, at longer days of treatment the values revealed a significant depletion. Initially its administration stimulated the uterine structures, caused metaplastic changes in the cervical epithelium and provoked considerable cornification in the vaginal epithelium. At later durations significant inhibition in the histoarchitecture was observed. Biochemical observations supplemented with the histological findings have been correlated with the anti-implantation action of the aqueous extract in the light of its hormonal properties. PMID:3227756

Shukla, S; Mathur, R; Prakash, A O

1988-01-01

176

Suppressive effects of Moringa oleifera Lam pod against mouse colon carcinogenesis induced by azoxymethane and dextran sodium sulfate.  

PubMed

Moringa oleifera Lam (horseradish tree; tender pod or fruits) is a major ingredient in Thai cuisine and has some medicinal properties. Previous studies have shown potentially antioxidant, antitumor promoter, anticlastogen and anticarcinogen activities both in vitro and in vivo. The present study was conducted to investigate chemopreventive effects on azoxymethane (AOM)-initiated and dextran sodium sulfate (DSS)-promoted colon carcinogenesis in mice. Male ICR mice were divided into 8 groups: Group 1 served as a negative control; Group 2 received AOM/DSS as a positive control; Groups 3-5 were fed boiled freeze-dried M. oleifera (bMO) at 1.5%, 3.0% and 6.0%, respectively supplemented in basal diets for 5 weeks; Groups 6-8 were fed with bMO diets at the designed doses above for 2 weeks prior to AOM, during and 1 week after DSS administration. At the end of the study, colon samples were processed for histopathological examination. PCNA indices, and iNOS and COX-2 expression were assessed by immunohistochemistry. The results demonstrated the incidences and multiplicities of tumors in Groups 6-8 to be decreased when compared to Group 2 in a dose dependent manner, but this was significant only in Group 8. The PCNA index was also significantly decreased in Group 8 whereas iNOS and COX-2 protein expression were significantly decreased in Groups 7 and 8. The findings suggest that M. oleifera Lam pod exerts suppressive effects in a colitis-related colon carcinogenesis model induced by AOM/DSS and could serve as a chemopreventive agent. PMID:22471457

Budda, Sirintip; Butryee, Chaniphun; Tuntipopipat, Siriporn; Rungsipipat, Anudep; Wangnaithum, Supradit; Lee, Jeong-Sang; Kupradinun, Piengchai

2011-01-01

177

Adsorption of bacteriophage lambda on the LamB protein of Escherichia coli K-12: point mutations in gene J of lambda responsible for extended host range.  

PubMed

LamB is the cell surface receptor for bacteriophage lambda. LamB missense mutations yielding resistance to lambda group in two classes. Class I mutants block the growth of lambda with the wild-type host range (lambda h+) but support the growth of one-step host range mutants (lambda h). Class II mutants block lambda h but support the growth of two-step host range mutant (lambda hh*) phages. To identify amino acid residues in the J protein (the tail fiber of phage lambda) responsible for the extended host range phenotype of mutants of phage (lambda h+), we selected a series of one-step (lambda h) and two-step (lambda hh*) host range mutants and analyzed their corresponding J genes. Three different class I LamB missense mutants (mutations at sites 247, 245, and 148) were used to select 11 independent, new, one-step host range mutants (lambda h phages). DNA sequence analysis revealed a single-amino-acid change in each case. The 11 alterations affected only three residues in the distal part of J, corresponding to a Val-->Ala change at site 1077 in five cases, a Thr-->Met change at site 1040 in three cases, and a Leu-->Pro change at site 1127 in three cases. Recombination experiments confirmed that in the cases tested, the mutations identified were indeed responsible for the extended host range phenotype. The class II LamB mutant (Gly-->Asp at site 151) was used to select two-step extended host range mutants (lambda hh* phages) from three new lambda h phages, corresponding to different amino acid modifications in the J protein (at sites 1040, 1077, and 1127). The new lambda hh* phages analyzed corresponded to either double or triple point mutations located at the distal end of the J protein. In all, seven residues involved in the extended host range properties of lambda mutants were identified in the distal part of the J protein, suggesting that the last C-terminal portion of the J protein participates directly in the adsorption of the phage onto LamB. In agreement with the fact that the lambda h mutants (and the lambda hh* mutants) could grow on all of the lamB class I mutations tested, we found tha the nature of the J mutations did not depend on the LamB class I mutant used to select them. This is interpreted as meaning that the mutated residues in the J protein and in the LamB mutants are not involved in allele-specific protein-protein interactions. Rather, the LamB mutations would block a step in phage adsorption, and this block would be overcome by the mutations in the J protein. PMID:8106335

Werts, C; Michel, V; Hofnung, M; Charbit, A

1994-02-01

178

Adsorption of bacteriophage lambda on the LamB protein of Escherichia coli K-12: point mutations in gene J of lambda responsible for extended host range.  

PubMed Central

LamB is the cell surface receptor for bacteriophage lambda. LamB missense mutations yielding resistance to lambda group in two classes. Class I mutants block the growth of lambda with the wild-type host range (lambda h+) but support the growth of one-step host range mutants (lambda h). Class II mutants block lambda h but support the growth of two-step host range mutant (lambda hh*) phages. To identify amino acid residues in the J protein (the tail fiber of phage lambda) responsible for the extended host range phenotype of mutants of phage (lambda h+), we selected a series of one-step (lambda h) and two-step (lambda hh*) host range mutants and analyzed their corresponding J genes. Three different class I LamB missense mutants (mutations at sites 247, 245, and 148) were used to select 11 independent, new, one-step host range mutants (lambda h phages). DNA sequence analysis revealed a single-amino-acid change in each case. The 11 alterations affected only three residues in the distal part of J, corresponding to a Val-->Ala change at site 1077 in five cases, a Thr-->Met change at site 1040 in three cases, and a Leu-->Pro change at site 1127 in three cases. Recombination experiments confirmed that in the cases tested, the mutations identified were indeed responsible for the extended host range phenotype. The class II LamB mutant (Gly-->Asp at site 151) was used to select two-step extended host range mutants (lambda hh* phages) from three new lambda h phages, corresponding to different amino acid modifications in the J protein (at sites 1040, 1077, and 1127). The new lambda hh* phages analyzed corresponded to either double or triple point mutations located at the distal end of the J protein. In all, seven residues involved in the extended host range properties of lambda mutants were identified in the distal part of the J protein, suggesting that the last C-terminal portion of the J protein participates directly in the adsorption of the phage onto LamB. In agreement with the fact that the lambda h mutants (and the lambda hh* mutants) could grow on all of the lamB class I mutations tested, we found tha the nature of the J mutations did not depend on the LamB class I mutant used to select them. This is interpreted as meaning that the mutated residues in the J protein and in the LamB mutants are not involved in allele-specific protein-protein interactions. Rather, the LamB mutations would block a step in phage adsorption, and this block would be overcome by the mutations in the J protein. PMID:8106335

Werts, C; Michel, V; Hofnung, M; Charbit, A

1994-01-01

179

Growth, root and leaf structure, and biomass allocation in Leucanthemum vulgare Lam. (Asteraceae) as influenced by heavy-metal-containing slag  

Microsoft Academic Search

Effects of heavy metal contamination on growth, leaf turnover, biomass allocation and leaf and root structure of Leucanthemum vulgare Lam. were investigated. Plants were grown in two outdoor experiments, for 5 weeks or for 3 months, respectively, on sand\\u000a with different additions of slag containing elevated levels of heavy metals, especially Cu and Ni. In the 3-month experiment\\u000a nutrients were provided as

Peter Ryser; Phil Emerson

2007-01-01

180

Seed yield components, oil content, and fatty acid composition of two cultivars of moringa ( Moringa oleifera Lam.) growing in the Arid Chaco of Argentina  

Microsoft Academic Search

Moringa oleifera Lam. (M. pterygosperma Gaertn [Moringaceae]) is a fast-growing small tree native to the sub-Himalayan tracts of Northern India. The recognition that moringa oil has value in cosmetics has increased interest in cultivating it for seed-oil. The experimental trials were conducted in a semi-commercial moringa plantation in the subtropical northwestern region of Argentina, considering the similar climate conditions to

Ricardo Ayerza

2011-01-01

181

Inhibitory Effect of n-butanol Fraction of Moringa oleifera Lam. Seeds on Ovalbumin-Induced Airway Inflammation in a Guinea Pig Model of Asthma  

Microsoft Academic Search

Moringaceae, which belongs to the Moringa oleifera Lam. family, is a well-known herb used in Asian medicine as an antiallergic drug. In the present study, the efficacy of the n-butanol extract of the seeds of the plant (MONB) is examined against ovalbumin-induced airway inflammation in guinea pigs. The test drugs (MONB or dexamethasone) are administered orally prior to challenge with

Shailaja G. Mahajan; Aryamitra Banerjee; Bhupendrasinh F. Chauhan; Harish Padh; Manish Nivsarkar; Anita A. Mehta

2009-01-01

182

Studies on seasonal variation of plant hormones, nitrogen flixation and indole acetic acid metabolism in root nodulus of Butea monosperma (Lam.) Taub  

Microsoft Academic Search

Summary Mature nodules ofButea monosperma (Lam.) Taub. (B. monosperma) contained higher amounts of indole acetic acid-like (IAA), cytokinin-like (CK), gibberellic acid-like (GA) and abscisic acid-like (ABA) substances, and were more active in IAA metabolism and nitrogen fixation. Roots contained lower amount of all the hormones. The higher level of IAA in the nodules than the roots was due to more

T. K. Dangar; P. S. Basu

1985-01-01

183

Morphology of leaves of cyanus segetum LAM ( Centaurea cyanus L.) after photoperiodic inhibition at different stages of ontogeny of the shoot apex  

Microsoft Academic Search

Cyanus segetum LAM. was transferred from a long to a short photoperiodic regime at various stages of ontogenesis and the development of\\u000a the plant investigated. The morphology of the leaves ofCyanus segetum was dependent on the photoperiodic regime. On a short photoperiodic regime, pinnately sected leaves were formed, but only\\u000a if development was inhibited while the shoot apex still had

Frideta Seidlová

1964-01-01

184

In situ trace-element analysis of individual silicate melt inclusions by laser ablation microprobe-inductively coupled plasma-mass spectrometry (LAM-ICP-MS)  

Microsoft Academic Search

This paper reports the successful application of laser ablation microprobe-inductively coupled plasma-mass spectrometry (LAM-ICP-MS) to the in situ analysis of a diverse suite of twenty trace elements including Zr, Hf, Nb, Ta, Y, and REEs, in individual silicate melt inclusions in phenocrysts from Fantale volcano, Ethiopia. The UV laser, a frequency quadrupled Nd: YAG operating at 266 nm, significantly improves

R. P. Taylor; S. E. Jackson; H. P. Longerich; J. D. Webster

1997-01-01

185

Pruning management effects on soil carbon and nitrogen in contour-hedgerow cropping with Leucaena leucocephala (Lam.) De Wit on sloping land in Haiti  

Microsoft Academic Search

Application of hedgerow prunings to the soil in alley cropping ispracticed to sustain crop yield but information is lacking on effects ofhedgerow management on soil C and N dynamics under continuous cropping.Cumulative effects of 3.5 years of leucaena [Leucaenaleucocephala (Lam.) De Wit] hedgerow management on soil organic CandN and potential C and N mineralization were determined in an alley croppingexperiment

L. Isaac; C. W. Wood; D. A. Shannon

2003-01-01

186

Glutathione peroxidase-like activity of 33 kDa trypsin inhibitor from roots of sweet potato ( Ipomoea batatas [L.] Lam ‘Tainong 57’)  

Microsoft Academic Search

The hydrogen peroxide, glutathione (GSH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phenazine methosulfate (PMS) were used for spectrophotometric assay of commercial glutathione peroxidase (GPx) activity at 570nm. A positive correlation (r2=0.998) was found between level of GPx activity and the absorbance changes (%). The 33kDa trypsin inhibitor (TI) was purified from the storage roots of sweet potato (SP) (Ipomoea batatas [L.] Lam

Wen-Chi Hou; Hsien-Jung Chen; Chuan-Hsiao Han; Ching-Yang Lin; Yaw-Huei Lin

2004-01-01

187

Flow injection spectrophotometric determination of sulfite using a crude extract of sweet potato root ( Ipomoea batatas (L.) Lam.) as a source of polyphenol oxidase  

Microsoft Academic Search

A flow injection (FI) spectrophotometric procedure is proposed for the determination of sulfite in white wine, white vinegar and juice samples, based on its inhibitory effect on the activity of polyphenol oxidase. As enzymatic source of polyphenol oxidase (PPO; tyrosinase, cathecol oxidase; E.C. 1.14.18.1) a crude extract of sweet potato root (Ipomoea batatas (L.) Lam.) was used directly in the

Orlando Fatibello-Filho; Iolanda da Cruz Vieira

1997-01-01

188

Influence of shading on net photosynthetic and transpiration rates, stomatal diffusive resistance, nitrate reductase and biomass productivity of a woody legume tree species ( Erythrina variegata Lam.)  

Microsoft Academic Search

In view of the sun and shade adaptations of plants to semi-arid climatic conditions investigations were undertaken withErythrina variegata Lam. The studies revealed that shading decreased root and shoot growth, leaf density, leaf area, specific leaf weight, relative\\u000a growth rate and relative leaf growth rate. Consequently the dry biomass accumulation was adversely affected in shade grown\\u000a plants. Synthesis of chlorophyll

K Muthuchelian; Kailash Paliwal; A Gnanam

1989-01-01

189

Preface: Proceedings of the 13th Conference on Liquid and Amorphous Metals (LAM13) (Ekaterinburg, Russia, 8 14 July 2007)  

NASA Astrophysics Data System (ADS)

The most recent developments in the field of liquid and amorphous metals and alloys are regularly updated through two complementary international conferences: the liquid and amorphous metals conference (LAM) and the rapidly quenched materials (RQ) conference. The first series of conferences started as LM1 in 1966 at Brookhaven for the basic understanding of liquid metals. The subsequent LM conferences were held in Tokyo (1972) and Bristol (1976). The conference was renewed in Grenoble (1980) as a LAM conference including amorphous metals and continued in Los Angeles (1983), Garmisch-Partenkirchen (1986), Kyoto (1989), Vienna (1992), Chicago (1995), Dortmund (1998), Yokohama (2001) and Metz (2004). The conferences are mainly devoted to liquid and amorphous metals and alloys. However, communications on some non-metallic systems such as semiconductors, quasicrystals etc, are also accepted. The conference tradition strongly encourages participation from junior researchers and graduate students. The 13th conference of the LAM series was organized in Ekaterinburg, Russia, by the Institute of Metallurgy of the Ural Branch of the Russian Academy of Sciences (IMet UB RAS) and the Ural State Pedagogical University (USPU), and held from 8-14 July 2007 under the chairmanship of Professors Pjotr Popel (USPU) and Boris Gelchinskii (IMet UB RAS). Two hundred and forty two active participants and about 60 guest participants from 20 countries attended the conference. There were no parallel sessions and all oral reports were separated into three groups: invited talks (40 min), full-scale oral reports (25 min), and brief oral reports (15 min). The program included ten sessions, ranging from purely theoretical subjects to the technological application of molten and amorphous alloys. The following sessions took place: A: Electronic structure and transport, magnetic properties; B: Phase transitions; C: Structure; D: Atomic dynamics and transport; E: Thermodynamics; F: Modelling, simulation; G: Surface and interface; H: Mechanical properties and new materials; I: Quasicrystals; J: Industrial applications. The largest sessions were E, C, D and F with 53, 47, 43 and 40 reports, respectively. Posters were exhibited during the first (sessions A-E) or last (sessions F-J) three days of the conference. As usual, each oral session started with an invited talk. In accordance with contemporary tendencies the first invited talk of M Yao (Kyoto University) was devoted to interrelations between liquid metal studies and nanoscience. He pointed out that the physics of disordered matter, especially liquid metals, has provided nanoscience with basic ideas and theoretical tools such as ab initio molecular dynamics (MD) simulation. K Tamura (Kyoto University) reported experimental investigation results of expanded liquid rubidium, showing that observed structural features are originated from the instability of low-density electron gas. Modern theory has predicted that interacting electron gas suffers negative compressibility and the static dielectric function (DF) becomes negative when electron density is sufficiently reduced. Negative DF is of special interest when considering the possibility of new types of superconductors. In the next invited lecture, M I Mendelev and J R Morris (USA) described how MD simulations were performed to study phase transformations in supercooled liquid Al. The authors found that the widely used EAM potential for Al enables vitrification for cooling rates achievable in classical MD simulation, while other Al potentials enable crystallization under the same conditions. The reason for this difference was discussed, as well as features of vitrification in the EA Al. P Häussler (Chemnitz Univeristy) spoke about fundamental structure-forming processes in liquid and amorphous materials. He proposed the resonance model as an autonomous missing link between the microscopic description of atoms/molecules of Schrödinger's equation, and crystals, where global concepts such as planar resonances exist and Bloch's theorem is applied. D Holland-

Popel, Pjotr; Gelchinskii, Boris; Sidorov, Valeriy

2008-03-01

190

The influence of deep frying using various vegetable oils on acrylamide formation in sweet potato (Ipomoea batatas L. Lam) chips.  

PubMed

The objective of this study was to evaluate the precursors of acrylamide formation in sweet potato (SP) (Ipomoea batatas L. Lam) chips and to determine the effect of different types of vegetable oils (VOs), that is, palm olein, coconut oil, canola oil, and soya bean oil, on acrylamide formation. The reducing sugars and amino acids in the SP slices were analyzed, and the acrylamide concentrations of SP chips were measured. SP chips that were fried in a lower degree of unsaturation oils contained a lower acrylamide concentration (1443 ?g/kg), whereas those fried with higher degree of unsaturated oils contained a higher acrylamide concentration (2019 ?g/kg). SP roots were found to contain acrylamide precursors, that is, 4.17 mg/g glucose and 5.05 mg/g fructose, and 1.63 mg/g free asparagine. The type of VO and condition used for frying, significantly influenced acrylamide formation. This study clearly indicates that the contribution of lipids in the formation of acrylamide should not be neglected. PMID:24344977

Lim, P K; Jinap, S; Sanny, M; Tan, C P; Khatib, A

2014-01-01

191

Protective Effect of Ethanolic Extract of Seeds of Moringa oleifera Lam. Against Inflammation Associated with Development of Arthritis in Rats.  

PubMed

The present investigation was carried out to study the anti-arthritic activity of ethanolic extract of seeds of Moringa oleifera Lam. (MOEE) in adjuvant-induced arthritis in adult female Wistar rats. During the experimental period, body weight, paw edema volume (primary lesion) and arthritic index (secondary lesion) was observed. On the 21st day, serum from each animal was used for estimation of Rheumatoid Factor (RF) value and levels of selected cytokines (TNFalpha, IL-1, and IL-6). Whole blood was used for measurement of erythrocyte sedimentation rate (ESR). Liver homogenate was utilized for assessment of oxidative stress and histopathology was performed to measure degree of inflammation in synovial joint. Our results suggest that, percentage reduction in body weight was less, paw edema volume and arthritic index score was decreased significantly as compared to diseased control animals. Serum levels of RF, TNF-alpha, IL-1, and IL-6 also showed decreased levels as compared to those in the diseased control group. Treatment with MOEE also altered oxidative stress in relation to its anti-inflammatory activity. Histopathological observations showed mild or less infiltration of lymphocytes, angiogenesis and synovial lining thickening. From all above results and observations, it can be concluded that Moringa oleifera possesses promising antiarthritic property. PMID:18958711

Mahajan, Shailaja G; Mali, Ravindra G; Mehta, Anita A

2007-01-01

192

Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats.  

PubMed

The leaves of Moringa oleifera Lam (Moringaceae) are used by the Indians in their herbal medicine as a hypocholesterolemic agent in obese patients. The scientific basis for their use in hypercholesterolemia was therefore examined. It was found that administration of the crude leaf extract of Moringa oleifera along with high-fat diet decreased the high-fat diet-induced increases in serum, liver, and kidney cholesterol levels by 14.35% (115-103.2 mg/100 ml of serum), 6.40% (9.4-8.8 mg/g wet weight) and 11.09% (1.09-0.97 mg/g wet weight) respectively. The effect on the serum cholesterol was statistically significant. No significant effect on serum total protein was observed. However, the crude extract increased serum albumin by 15.22% (46-53 g/l). This value was also found to be statistically significant. It was concluded that the leaves of Moringa oleifera have definite hypocholesterolemic activity and that there is valid pharmacological basis for employing them for this purpose in India. PMID:10661880

Ghasi, S; Nwobodo, E; Ofili, J O

2000-01-01

193

Effects of Oral Administration of Moringa oleifera Lam on Glucose Tolerance in Goto-Kakizaki and Wistar Rats.  

PubMed

Medicinal plants constitute an important source of potential therapeutic agents for diabetes. In the present study, we investigated the effects of Moringa oleifera (MO) Lam, Moringacea, on glucose tolerance in Wistar rats and Goto-Kakizaki (GK) rats, modeled type 2 diabetes. Major polyphenols in MO powder were quercetin glucosides, rutin, kaempferol glycosides and chlorogenic acids by HPLC analysis. As the results of glucose tolerance test, MO significantly decreased the blood glucose at 20, 30, 45and 60 min for GK rats and at 10, 30 and 45 min for Wistar rats (p<0.05) compared to the both controls after glucose administration. The area under the curve of changes in the blood glucose was significantly higher in the GK control group than in the GK plus MO group (p<0.05) in the periods 30-60 min and 60-120 min. Furthermore, MO significantly decreased stomach emptying in GK rats (p<0.05). The results indicated that MO has an ameliorating effect for glucose intolerance, and the effect might be mediated by quercetin-3-glucoside and fiber contents in MO leaf powder. The action of MO was greater in GK rats than in Wistar rats. PMID:18398501

Ndong, Moussa; Uehara, Mariko; Katsumata, Shin-Ichi; Suzuki, Kazuharu

2007-05-01

194

Total Phenolics and Total Flavonoids Contents and Hypnotic Effect in Mice of Ziziphus mauritiana Lam. Seed Extract  

PubMed Central

The seeds of Ziziphus mauritiana Lam. have been traditionally used for treatment of various complications including insomnia and anxiety. They are popularly used as sedative and hypnotic drugs in China, Korea, Myanmar, Vietnam, and other Asian countries. However, no scientific proof on hypnotic activity of Z. mauritiana seeds (ZMS) was reported. In this study, the hypnotic activity of 50% ethanolic extract from ZMS was observed on the loss of righting reflex in mice using pentobarbital-induced sleep mice method. The contents of total phenolics and total flavonoids in the extract were also determined. The results showed that the 50% ethanolic extract from ZMS contained total phenolics 27.62 ± 1.43?mg gallic acid equivalent (GAE)/g extract and total flavonoids 0.74 ± 0.03?mg quercetin equivalent (QE)/g extract. Oral administration of the extract at the dose of 200?mg/kg significantly increased the sleeping time in mice intraperitoneally administered with sodium pentobarbital (50?mg/kg body weight). These results supported the traditional use of ZMS for the treatment of insomnia. The seeds of Z. mauritiana should be further developed as an alternative sedative and/or hypnotic product. PMID:23861716

San, Aye Moh Moh; Thongpraditchote, Suchitra; Sithisarn, Pongtip; Gritsanapan, Wandee

2013-01-01

195

Effect of Butea monosperma Lam. leaves and bark extracts on blood glucose in streptozotocin-induced severely diabetic rats  

PubMed Central

Background: Diabetes mellitus is a chronic metabolic disorder that has significant impact on the health, quality of life and life expectancy, as well as on the health care system. Butea monosperma Lam. Kuntze (Fabaceae), commonly known as palash, is widely used in the treatment of various diseases and disorders including diabetes. Materials and Methods: The present study was planned to evaluate the antidiabetic effect of aqueous extracts of B. monosperma leaves and bark in streptozotocin-induced severely diabetic rats. The animals were divided into four groups, with each consisting of six rats, viz. control, diabetic control, leaf extract-treated and bark extract-treated groups. Treatment was continued for 6 weeks. The biochemical estimations included blood glucose and serum insulin levels. Histopathology of pancreas was also performed. Results: The results indicated that both leaf and bark extracts of B. monosperma produced insignificant antihyperglycemic activity. The leaf and bark extracts reduced blood glucose to an extent of 28% and 11%, respectively. It was also evidenced that both leaf and bark extracts did not increase insulin synthesis or secretion and did not improve pancreatic architecture as reflected by the histopathologic studies. Conclusions: The findings of the study emphasize that B. monosperma does not possess significant antidiabetic activity in severe experimental diabetes at the dosage tested. PMID:22224059

Ahmed, Faiyaz; Siddaraju, N. S.; Harish, M.; Urooj, Asna

2012-01-01

196

Long-range sources of Toronto particulate matter (PM 2.5) identified by Aerosol Laser Ablation Mass Spectrometry (LAMS)  

NASA Astrophysics Data System (ADS)

An aerosol laser ablation mass spectrometer (LAMS) was operated continuously in downtown Toronto for a period of 11 months. The mass spectra of individual particles were classified according to their chemical composition using neural net-based adaptive resonance theory (ART-2a). The types of particles thereby identified were used as the basis for classifying particles from a 10-day sampling period within the 11-month sampling study. The particles within a class were further segregated by size, and the number of particles analyzed per hour was calculated for each of the resulting composition and sized based classes. ART-2a was used to combine these classes into groups based on similarity in their temporal trends. Two unusual sources were thereby identified: forest fire particles and intercontinental dust. The forest fire particles primarily exhibited potassium and potassium sulphate, while the intercontinental dust exhibited crustal metals, like titanium, aluminum, and iron. Back trajectory analysis of the air masses that transported these particles to Toronto revealed their origins as Northern Canada and Africa. From the chemical composition and size of the particles, a forest fire episode in Western Canada was identified during the 11-month sampling study on 1 June 2002. Other episodes of particles originating from Africa were not observed. These findings demonstrated the use of temporal variations in single particle chemistry to identify sources.

Owega, Sandy; Evans, Greg J.; Jervis, Robert E.; Fila, Mike; D'Souza, Ryan; Khan, Badi-Uz-Zaman

197

Validation of turbulence and convective schemes on western Africa; comparison of LAM and CRM simulations on an AMMA case study.  

NASA Astrophysics Data System (ADS)

The aim of this work is to analyse the behaviour of turbulence and convective parameterizations included in the Météo-France ALADIN-CLIMAT Limited Area Model in the frame of a 48 hour simulation of an AMMA case study, in comparison to observations and to a CRM (Méso-NH, with a 5 km horizontal grid-mesh) simulation carried out under the same boundary forcings. This framework provides an intermediate step of parameterization validation between the Single Column Model and Global Climate Model simulation studies. The chosen case study is the 26-27th July 2006 over a 43° x 40° region centred over Burkina Faso, in continuation to a previous work done with a HAPEX-Sahel case-study. During this 2 day period, two successive mesoscale convective systems are located ahead and in phase with the trough of an African easterly Wave (AEW). Both LAM and CRM simulations have been performed over the same considered domain, using the same ECMWF boundary forcings. Sensitivity tests to resolution (both horizontal and vertical) have been first carried out with ALADIN-CLIMAT. Second, the two different convection schemes used in ALADIN-CLIMAT show two kinds of response mainly due to their different formulations of triggering (no constraints in the dry layer under the convective cloud versus continuous treatment of convection including this dry layer) and closure (moisture convergence versus CAPE). Third, the impact of convective downdrafts will be shown. Fourth, the impact of different boundary forcing fields will also be presented.

Pollack, David; Gueremy, Jean-Francois; Beau, Isabelle

2010-05-01

198

Antinociceptive effect of an ethanolic extract of the aerial parts of Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae)  

PubMed Central

Background: Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae) is a perennial herb used in Ghanaian traditional medicine for the treatment of various painful conditions. Little scientific evidence exists in literature on the effect of this plant on pain. Materials and Methods: The present study examined the antinociceptive effect of the ethanolic extract of the aerial parts of H. latifolia in chemical (acetic acid-induced abdominal writhing, glutamate, formalin, and capsaicin tests) and thermal (tail immersion test) behavioral pain models in rodents. The possible mechanisms of the antinociceptive action were also assessed with various antagonists in the formalin test. Results: The H. latifolia extract (HLE) together with morphine and diclofenac (positive controls), showed significant antinociceptive activity in all the models used. The antinociceptive effect exhibited by HLE in the formalin test was partly or wholly reversed by the systemic administration of naloxone, theophylline, and atropine. Glibenclamide, ondansetron, yohimbine, nifedipine, and NG-L-nitro-arginine methyl ester (L-NAME), however, did not significantly block the antinociceptive effect of the extract. HLE, unlike morphine, did not induce tolerance to its antinociceptive effect in the formalin test after chronic administration; morphine tolerance did not also cross-generalize to HLE. Interestingly, also, the chronic concomitant administration of HLE and morphine significantly suppressed the development of morphine tolerance. Conclusion: Together, these results indicate that HLE produces dose-related antinociception in several models of chemical and thermal pain, without tolerance induction, through mechanisms that involve an interaction with adenosinergic, muscarinic cholinergic, and opioid pathways. PMID:21966159

Woode, Eric; Abotsi, Wonder K. M.

2011-01-01

199

Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis  

PubMed Central

Objectives Moxifloxacin (MXF) has been advocated for the treatment of extensively drug-resistant (XDR) tuberculosis despite resistance to older-generation fluoroquinolones. We investigated the relationship between the minimum inhibitory concentration (MIC) of MXF and mutations in the gyrA and gyrB genes in Mycobacterium tuberculosis (MTB) isolates from KwaZulu-Natal (KZN) Province of South Africa. Materials and methods MICs of 56 MTB isolates were compared to the mutations in the quinolone resistance-determining region known to confer fluoroquinolone resistance. Isolates were genotyped by IS6110 restriction fragment length polymorphism analysis. Results The circulating F15/LAM4/KZN XDR strain circulating in KZN Province harbored the A90V mutation and displayed high-level resistance with MICs of 8 mg/L for ciprofloxacin and ofloxacin and ?1 mg/L for MXF. Conclusion The inclusion of MXF in XDR-TB treatment regimens requires careful consideration in our setting, where clinical outcome data in MXF-containing regimens are unavailable.

Dookie, Navisha; Sturm, A Willem; Moodley, Prashini

2014-01-01

200

Immunogenicity of viral B-cell epitopes inserted into two surface loops of the Escherichia coli K12 LamB protein and expressed in an attenuated aroA strain of Salmonella typhimurium.  

PubMed

We previously developed a general procedure which allows the genetic coupling of a chosen foreign linear epitope in different 'permissive' sites of a carrier protein. By using the outer membrane protein LamB of Escherichia coli K12 as a carrier, we were able to express a number of different foreign epitopes at the bacterial surface. In the present work, taking advantage of the recent determination of the crystal structure of LamB, we inserted two model B-cell epitopes i.e.--the C3 epitope from poliovirus (residues 93 to 103 of VP1) and the preS2 epitope from hepatitis B virus, (residues 132 to 145)--at the tip of the most distal and largest surface exposed region of LamB (after residues 386, into loop L9). We also used two previously constructed LamB hybrids, corresponding to the insertion of the C3B or preSB epitope into permissive site 153 (lying in the middle of the fourth surface loop of LamB), to construct two LamB proteins corresponding to the simultaneous insertion of the two different epitopes (with one epitope per site). The LamB hybrids were placed under the control of the anaerobically inducible pnirB promoter and expressed in a LamB-negative derivative of the aroA attenuated strain of S. typhimurium, SL3261. In vitro, the recombinant proteins were expressed at a high level (up to 10% of whole cell proteins) and in vivo the recombinant plasmids were stably maintained. For both epitopes, genetic coupling at site 386 appeared to be more favorable for the induction of anti-epitope antibodies than coupling at site 153. Moreover, the LamB hybrid corresponding to the simultaneous insertion of the preSB epitope at site 153 and of the C3B epitope at site 386 allowed the induction of both anti-poliovirus and anti-hepatitis B antibodies. PMID:10078601

Wang, J; Michel, V; Leclerc, C; Hofnung, M; Charbit, A

1999-01-01

201

Trace element partition coefficients for clinopyroxene and phlogopite in an alkaline lamprophyre from Newfoundland by LAM-ICP-MS  

NASA Astrophysics Data System (ADS)

Mineral/rock matrix partition coefficients have been determined for clinopyroxene (Cpx) and phlogopite from a Mesozoic alkaline lamprophyre from Newfoundland, Canada, by Laser Ablation Microprobe (LAM-ICP-MS). Values for twenty-one elements were obtained for Cpx, whereas only eleven were possible for phlogopite due to REE abundances below detection limits ( 1 ppm). Ablation pits of 40-50 ?m diameter enabled investigation of zonation in phenocryst phases. In general, phenocrysts exhibit little trace element zonation except in the outermost overgrowths of Cpx. In these, a fourfold to fivefold increase in many trace element abundances correlates strongly with increasing Ti and Al contents, in agreement with recent experimental studies. Only Li shows appreciable zonation in phlogopite, being enriched in the rims. Comparison of the partition coefficients determined by in-situ laser analysis with those obtained from apparently pure mineral separates by solution ICP-MS indicates that, for several elements, considerable differences exist, e.g., DBa 0.0006 vs. 0.0255. These differences are attributable to the inclusion of trace element-rich overgrowths, and zones of trace element enrichment and micro-inclusions, in addition to the possibility of small amounts of matrix or glass (1-2%) in the nominally clean clinopyroxene mineral separates. Partition coefficients for Cpx are lower than most published values for basaltic rocks, but are comparable to experimental values for basalt determined by SIMS. Comparison with experimental values from lamproite discounts a strong bulk compositional effect, so that the discrepancy with earlier values for basalt should probably be attributed to problems with mineral separates, emphasizing the need for high quality partitioning determinations with in-situ microbeam methods. The phlogopite data extend considerably the published range; the new values are also generally lower than published values, although the discrepancy here may be due to bulk compositional effects, as many published values are from more silicic systems. The lamprophyre values are within the range of sparse experimentally determined values.

Foley, Stephen F.; Jackson, Simon E.; Fryer, Brian J.; Greenouch, John D.; Jenner, George A.

1996-02-01

202

Evaluation of a cost effective technique for treating aquaculture water discharge using Lolium perenne Lam as a biofilter.  

PubMed

Wastewater stabilization ponds generate low cost by-products that are useful for agriculture. The utilization of these by-products for soil amendment and as a source of nutrients for plants requires a high level of sanitation and stabilization of the organic matter, to maintain acceptable levels of soil, water and air quality. In this study, two aquaculture wastewater treatment systems; recirculating system and a floating plant bed system were designed to improve the quality of irrigation water in local communities with low income. In both systems the grass species Lolium perenne Lam was used as a plant biofilter while vegetable specie Amaranthus viridis was used to evaluate the performance of the system and the suitability of the phyto-treated water for irrigation. It was found that the harmful material removal rate for recirculating system was 88.9% for TAN (total ammonia nitrogen), 90% for NO2(-)-N, 64.8% for NO3(-)-N while for floating plant bed system 82.7% for TAN, 82% for NO2(-)-N and 60.5% for NO3(-)-N. Comparative analysis of the efficiency of waste element removal between the two systems revealed that both systems performed well, however, plant growth was not robust for floating plant bed system while recirculating system is energy consuming. Although both systems did not attain sufficient levels of TN (total nitrogen) and TP (total phosphorus) load reduction, the treatment with L. perenne remarkably improved the irrigation water quality. A. viridis plants irrigated with the phyto-treated discharge water had lesser concentrations of heavy metals in their tissues compared to those irrigated with untreated discharge. The control plants irrigated with untreated discharge were also found to be highly lignified with few stems and small leaves. PMID:17966513

Nduwimana, André; Yang, Xiang-Long; Wang, Li-Ren

2007-01-01

203

Isolation, characterization and antimicrobial evaluation of a novel compound N-octacosan 7? ol, from Fumaria parviflora Lam  

PubMed Central

Background Fumaria parviflora Lam. (Fumaraceae) is widely used in traditional as well as folkloric system of medicine from ancient. It is commonly known as ‘Pitpapra’ or ‘Shahtrah’ in Indian traditional system of medicine and used for treating numerous ailments like diarrhea, fever, influenza, blood purifier and other complications. The object of the present study was to evaluate the Antileishmanial, antibacterial, antifungal and cytotoxic potential of isolated compound. Methods Methanolic extract of whole plant of Fumaria parviflora was dried under reduced pressure to obtain a dark brown residue which was adsorbed on silica gel column grade (60–120 mesh) to obtain a slurry and chromatographed over silica gel loaded column in petroleum ether – chloroform (3:1, 1:1 and 1:3?v/v). The in vitro antileishmanial evaluation of isolated compound against Leishmania donovani promastigotes was investigated by growth kinetics assay, reversibility assay, analysis of cellular morphology, adverse toxicity and determination of 50% growth inhibitory concentration (GI50). Disc diffusion and broth micro dilution methods were used to study the antibacterial (Gram?+?Staphylococcus epidermidis and Bacillus subtilis; Gram - Escherichia coli and Salmonella typhimurium) and antifungal (Candida albicans and Aspergillus niger) potential in vitro. Results Structure elucidation by spectral data analysis revealed a novel compound, n-octacosan-7?-ol (OC), yield (0.471%), having significant antimicrobial activity against Leishmania donovani promastigotes, Staphylococcus epidermidis, Escherichia coli, Candida albicans and Aspergillus niger in vitro with GI50?=?5.35, MIC 250, MIC 250 and MFC 500 and MIC 250 ?g ml-1 respectively. The isolated compound did not show adverse effect against mammalian macrophages. Conclusions The available evidence of compound suggested that it may be used as antimicrobial agent in future and may provide new platform for drug discovery programmes for leishmaniasis. PMID:24621260

2014-01-01

204

Digital Gene Expression Analysis Based on Integrated De Novo Transcriptome Assembly of Sweet Potato [Ipomoea batatas (L.) Lam.  

PubMed Central

Background Sweet potato (Ipomoea batatas L. [Lam.]) ranks among the top six most important food crops in the world. It is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. However, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. Hence, studies based on high-throughput sequencing technologies are needed to get a comprehensive and integrated genomic resource and better understanding of gene expression patterns in different tissues and at various developmental stages. Methodology/Principal Findings Illumina paired-end (PE) RNA-Sequencing was performed, and generated 48.7 million of 75 bp PE reads. These reads were de novo assembled into 128,052 transcripts (?100 bp), which correspond to 41.1 million base pairs, by using a combined assembly strategy. Transcripts were annotated by Blast2GO and 51,763 transcripts got BLASTX hits, in which 39,677 transcripts have GO terms and 14,117 have ECs that are associated with 147 KEGG pathways. Furthermore, transcriptome differences of seven tissues were analyzed by using Illumina digital gene expression (DGE) tag profiling and numerous differentially and specifically expressed transcripts were identified. Moreover, the expression characteristics of genes involved in viral genomes, starch metabolism and potential stress tolerance and insect resistance were also identified. Conclusions/Significance The combined de novo transcriptome assembly strategy can be applied to other organisms whose reference genomes are not available. The data provided here represent the most comprehensive and integrated genomic resources for cloning and identifying genes of interest in sweet potato. Characterization of sweet potato transcriptome provides an effective tool for better understanding the molecular mechanisms of cellular processes including development of leaves and storage roots, tissue-specific gene expression, potential biotic and abiotic stress response in sweet potato. PMID:22558397

Wang, Hai-Yan; Zheng, Wen; Li, Xiao; Zhao, Chuan-Wu; Zhang, Yi-Zheng

2012-01-01

205

Ontogenetic Variation of Total Phenolics and Antioxidant Activity in Roots, Leaves and Flowers of Astragalus compactus Lam. (Fabaceae)  

PubMed Central

Introduction The potential health risks and toxicity of synthetic antioxidants resulted in an upsurge of interest in phytochemicals as new sources of natural antioxidants. Phenolics of Astragalus L. (Fabaceae) possess antioxidant properties and have been shown to have a protective effect against several degenerative diseases. The objective of this study was to determine total phenolics and antioxidant activity of methanolic extracts from different parts of A. compactus Lam. at different phenological phases and to investigate the correlations between antioxidation and the contents of the total phenolics. Methods Total phenolic content (TPC) was determined using the Folin-Ciocalteau reagent and the antioxidant capacity was evaluated with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. Results Generally, the TPC in leaves was higher than that of the roots and flowers. TPC in leaves, roots and flowers of the species varied from 5.01-8.25, 4.29-7.89 and 4.19 ?g GAE/mg DW, respectively. In addition, roots and leaves at fructification stage possessed higher TPC than vegetative and flowering stages. Therefore, the leaf extracts at fructification phase showed the highest TPC that accompanied with best antioxidant activity. In the root extracts, fructification stage was also characterized by the highest antioxidant activity. Conclusion A positive relationship between antioxidant activity and TPC showed that phenolics were the dominant antioxidant components in the species. The results obtained suggest that A. compactus methanolic extracts may serve as potential sources of natural phenolic antioxidants and that the fructification phase could be considered as the best stage for the harvesting of this plant. PMID:23678448

Naghiloo, Somayeh; Movafeghi, Ali; Delazar, Abbas; Nazemiyeh, Hossein; Asnaashari, Solmaz; Dadpour, Mohammad Reza

2012-01-01

206

Triazine-based mesoporous covalent imine polymers as solid supports for copper-mediated Chan-Lam cross-coupling N-arylation reactions.  

PubMed

The synthesis of a novel mesoporous covalent imine polymeric (MCIPs) material, involving simple Schiff-base chemistry, is reported. This highly functionalised nitrogen-rich material acts as a good support for immobilising Cu(II) ions, exhibiting excellent catalytic activity in promoting the Chan-Lam cross-coupling reaction between biologically active amines and arylboronic acids. The performance of this catalyst is also evident from its broad substrate scope, high stability, real heterogeneity, mild reaction conditions and reusability without loss of activity. The observed results will provide additional scope on the design and catalytic applications of this emerging class of materials. PMID:24938420

Puthiaraj, Pillaiyar; Pitchumani, Kasi

2014-07-01

207

Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolising enzymes, antioxidant parameters and skin papillomagenesis in mice.  

PubMed

The modulatory effects of a hydro-alcoholic extract of drumsticks of Moringa oliefera Lam at doses of 125 mg/kg bodyweight and 250 mg/ kg body weight for 7 and 14 days, respectively, were investigated with reference to drug metabolising Phase I (Cytochrome b(5) and Cytochrome p(450) ) and Phase II (Glutathione-S- transferase) enzymes, anti-oxidant enzymes, glutathione content and lipid peroxidation in the liver of 6-8 week old female Swiss albino mice. Further, the chemopreventive efficacy of the extract was evaluated in a two stage model of 7,12 - dimethylbenz(a)anthracene induced skin papillomagenesis. Significant increase (p<0.05 to p<0.01) in the activities of hepatic cytochrome b(5), cytochrome p(450), catalase, glutathione peroxidase ( GPx ), glutathione reductase (GR), acid soluble sulfhydryl content (-SH ) and a significant decrease ( p<0.01 ) in the hepatic MDA level were observed at both dose levels of treatment when compared with the control values. Glutathione-S- transferase ( GST )activity was found to be significantly increased (p<0.01 ) only at the higher dose level. Butylated hydroxyanisol (BHA ) fed at a dose of 0.75% in the diet for 7 and 14 days (positive control ) caused a significant increase (p<0.05 to p<0.01) in the levels of hepatic phase I and phase II enzymes, anti- oxidant enzymes, glutathione content and a decrease in lipid peroxidation. The skin papillomagenesis studies demonstrated a significant decrease (p<0.05 ) in the percentage of mice with papillomas, average number of papillomas per mouse and papillomas per papilloma bearing mouse when the animals received a topical application of the extract at a dose of 5mg/ kg body weight in the peri-initiation phase 7 days before and 7 days after DMBA application, Group II ), promotional phase (from the day of croton oil application and continued till the end of the experiment, Group III ) and both peri and post initiation stages (from 7 days prior to DMBA application and continued till the end of the experiment, Group IV) compared to the control group (Group I ). The percentage inhibition of tumor multiplicity has been recorded to be 27, 72, and 81 in Groups II, III, and IV, respectively. These findings are suggestive of a possible chemopreventive potential of Moringa oliefera drumstick extract against chemical carcinogenesis. PMID:12875626

Bharali, Rupjyoti; Tabassum, Jawahira; Azad, Mohammed Rekibul Haque

2003-01-01

208

Effect of Moringa oleifera Lam. seed extract on toluene diisocyanate-induced immune-mediated inflammatory responses in rats.  

PubMed

Moringa oleifera Lam. is a small tree cultivated throughout India. We have investigated the effect of ethanolic extract of seeds of Moringa oleifera (MOEE, an herbal remedy) on the potential prevention of immune-mediated inflammatory responses in toluene diisocyanate (TDI as antigen)-induced asthma in Wistar rats. Rats were divided into five different groups (n = 8/group): Group-I = unsensitized control; Group-II = TDI control/vehicle; Group-III = dexamethasone (DXM) 2.5 mg/kg; and, Groups IV and V = 100 mg/kg and 200 mg/kg body weight [BW] of MOEE, respectively. All rats (except unsensitized controls) were sensitized by intranasal application of 10% TDI to induce airway hypersensitivity. Animals in Groups II-V were given their respective drug treatment per os from 1 wk prior to initiation of sensitization until the day of final provocation with 5% TDI. After this last challenge, all rats were examined for hyperreactivity symptoms and then sacrificed to determine their total and differential leucocytes in blood and bronchoalveolar (BAL) fluid and levels of TNF proportional, variant, IL-4, and IL-6 in their BAL and serum. Homogenates of one lung lobe from each animal were utilized to assess oxidative stress; a separate lobe underwent histologic examination to assess airway inflammatory status. The results suggest that asthmatic symptoms were found in TDI control rats only, while both MOEE- and DXM-treated rats did not manifest any airway abnormality. In MOEE- and DXM-treated rats, neutrophil and eosinophil levels in the blood were decreased significantly; levels of total cells and each different cell in their BAL fluid were markedly decreased as compared to those in TDI controls. TNF alpha, IL-4, and IL-6 were predominant in serum as well as in BAL fluids in TDI controls, but these levels were reduced significantly by MOEE treatment. The antioxidant activity in relation to antiinflammatory activity of the extract and histopathological observations also reflected a protective effect. Based on the above findings and observations, it can be concluded that Moringa oleifera may possess some beneficial properties that act against chemically stimulated immune-mediated inflammatory responses that are characteristic of asthma in the rat. PMID:18958717

Mahajan, Shailaja G; Mali, Ravindra G; Mehta, Anita A

2007-04-01

209

LamB as a carrier molecule for the functional exposition of IgG-binding domains of the Staphylococcus aureus Protein A at the surface of Escherichia coli K12  

Microsoft Academic Search

One, two or four IgG-binding domains of the Staphylococcus aureus Protein A (SPA) were inserted into the LamB protein which was expressed under control of the tac promoter. The chimeric proteins were shown to be exposed at the cell surface by analysis of isolated outer membranes and also by testing their functional interaction with IgG molecules. We hereby show that

Lothar Steidler; Erik Remaut; Walter Fiers

1993-01-01

210

Site-directed mutagenesis of tyrosine 118 within the central constriction site of the LamB (maltoporin) channel of Escherichia coli. II. Effect on maltose and maltooligosaccharide binding kinetics.  

PubMed Central

The 3-D structure of the maltooligosaccharide-specific LamB channel of Escherichia coli (also called maltoporin) is known from x-ray crystallography. The central constriction of the channel formed by the external loop 3 is controlled by tyrosine 118. Y118 was replaced by site-directed mutagenesis by 10 other amino acids (alanine (A), isoleucine (I), asparagine (N), serine (S), cysteine (C), aspartic acid (D), arginine (R), histidine (H), phenylalanine (F), and tryptophan (W)) including neutral ones, negatively and positively charged amino acids to study the effect of their size, their hydrophobicity index, and their charge on maltose and maltooligosaccharide binding to LamB. The mutants were reconstituted into lipid bilayer membranes and the stability constants for binding of maltose, maltotriose, maltopentaose, and maltoheptaose to the channel were measured using titration experiments. The mutation of Y118 to any other non-aromatic amino acid led to a substantial decrease of the stability constant of binding by factors between about two and six. The highest effect was observed for the mutant Y118A. Replacement of Y118 by the two other aromatic amino acids, phenylalanine (F) and tryptophan (W), resulted in a substantial increase of the stability constant maximally by a factor of almost 400 for the Y118W mutant. The carbohydrate-induced block of the channel function was used for the study of current noise through the different mutant LamB channels. The analysis of the power density spectra allowed the evaluation of the on- and off-rate constants (k(1) and k(-1)) of sugar binding. The results suggest that both rate constants were affected by the mutations. For most mutants, with the exception of Y118F and Y118W, k(1) decreased and k(-1) increased, whereas the opposite was found for the aromatic amino acid mutants. The results suggest that tyrosine 118 has a crucial effect on carbohydrate transport through LamB. PMID:12080122

Orlik, Frank; Andersen, Christian; Benz, Roland

2002-01-01

211

Stress responses of duckweed (Lemna minor L.) and water velvet (Azolla filiculoides Lam.) to anionic surfactant sodium-dodecyl-sulphate (SDS).  

PubMed

Surfactants are used for several purposes and recently they have attracted the attention for their ability to modify the behavior of other preexistent or co-disposed contaminants, although their use or discharge in wastewaters can represent a real or potential risk for the environment. Lemna minor L. and Azolla filiculoides Lam. are floating aquatic macrophytes, very effective in accumulating several pollutants including sodium dodecyl sulphate (SDS). In this work we evaluated the effects of SDS on these species by determining the stress ethylene production via laser-based trace gas detection, and the activities of enzymes involved in stress response, such as guaiacol peroxidase (G-POD), phenylalanine ammonia-lyase (PAL) and polyphenol-oxidase (PPO). Phenolics content was also determined. The macrophytes were treated with different concentrations of SDS for one week. SDS affected duckweed enzymatic activities and phenol content. While in the fern phenolics amount, PAL, G-POD and PPO activities were not affected by SDS except for 100 ppm SDS, the only concentration that was taken up and not completely degraded. Stress ethylene production was induced only in the fern treated with 50 and 100 ppm SDS. PMID:22277247

Forni, C; Braglia, R; Harren, F J M; Cristescu, S M

2012-04-01

212

Spectrophotometric determination of methyldopa and dopamine in pharmaceutical formulations using a crude extract of sweet potato root (Ipomoea batatas (L.) Lam.) as enzymatic source.  

PubMed

A rapid, precise and low cost spectrophotometric method is proposed for the determination of methyldopa and dopamine in pharmaceutical formulations. The crude extract of sweet potato root (Ipomoea batatas (L.) Lam.) was used as an enzymatic source of polyphenol oxidase (PPO; EC.1.14.18.1). This enzyme catalyses the oxidation of catecholamines to the corresponding methyldopaquinone and dopaminequinone. Those compounds are converted by a rapid spontaneous auto-oxidation to methyldopachrome and dopaminechrome which have a strong absorption at 480 or 470 nm, respectively. The calibration graphs are linear from 2.0x10(-4) to 6.0x10(-3) M. The results obtained by the proposed enzymatic method are in close agreement with those obtained using a Pharmacopoeia procedure and also with the label values. The detection limit (three times the signal blank/slope) was 3.4x10(-5) and 3.0x10(-5) M for methyldopa and dopamine, respectively, the recovery of methyldopa and dopamine from three samples ranged from 97.5 to 102.9% of the added amount. PMID:18967177

da Cruz Vieira, I; Fatibello-Filho, O

1998-08-01

213

Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam.  

PubMed

Medicinal plants have been shown to have both chemopreventive and/or therapeutic effects on cancer and other diseases related to oxidative damage. Moringa oleifera Lam., known in the Hausa and Igala languages of Nigeria as "Zogale" and "Gergedi," respectively, and drumstick in English, is a plant that is used both as food and in folkloric medicine in Nigeria and elsewhere. Different parts of the plant were analyzed for polyphenol content as well as in vitro antioxidant potential. The methanol extract of the leaves of M. oleifera contained chlorogenic acid, rutin, quercetin glucoside, and kaempferol rhamnoglucoside, whereas in the root and stem barks, several procyanidin peaks were detected. With the xanthine oxidase model system, all the extracts exhibited strong in vitro antioxidant activity, with 50% inhibitory concentration (IC(50)) values of 16, 30, and 38 microL for the roots, leaves, and stem bark, respectively. Similarly, potent radical scavenging capacity was observed when extracts were evaluated with the 2-deoxyguanosine assay model system, with IC(50) values of 40, 58, and 72 microL for methanol extracts of the leaves, stem, and root barks, respectively. The high antioxidant/radical scavenging effects observed for different parts of M. oleifera appear to provide justification for their widespread therapeutic use in traditional medicine in different continents. The possibility that this high antioxidant/radical scavenging capacity may impact on the cancer chemopreventive potential of the plant must be considered. PMID:20521992

Atawodi, Sunday E; Atawodi, Joy C; Idakwo, Gabriel A; Pfundstein, Beate; Haubner, Roswitha; Wurtele, Gerd; Bartsch, Helmut; Owen, Robert W

2010-06-01

214

Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves.  

PubMed

Water, aqueous methanol, and aqueous ethanol extracts of freeze-dried leaves of Moringa oleifera Lam. from different agroclimatic regions were examined for radical scavenging capacities and antioxidant activities. All leaf extracts were capable of scavenging peroxyl and superoxyl radicals. Similar scavenging activities for different solvent extracts of each collection were found for the stable 1,1-diphenyl 2-picrylhydrazyl (DPPH(*)) radical. Among the three different moringa samples, both methanol and ethanol extracts of Indian origins showed the highest antioxidant activities, 65.1 and 66.8%, respectively, in the beta-carotene-linoleic acid system. Nonetheless, increasing concentration of all the extracts had significantly (P < 0.05) increased reducing power, which may in part be responsible for their antioxidant activity. The major bioactive compounds of phenolics were found to be flavonoid groups such as quercetin and kaempferol. On the basis of the results obtained, moringa leaves are found to be a potential source of natural antioxidants due to their marked antioxidant activity. This is the first report on the antioxidant properties of the extracts from freeze-dried moringa leaves. Overall, both methanol (80%) and ethanol (70%) were found to be the best solvents for the extraction of antioxidant compounds from moringa leaves. PMID:12670148

Siddhuraju, Perumal; Becker, Klaus

2003-04-01

215

Inhibitory effect of n-butanol fraction of Moringa oleifera Lam. seeds on ovalbumin-induced airway inflammation in a guinea pig model of asthma.  

PubMed

Moringaceae, which belongs to the Moringa oleifera Lam. family, is a well-known herb used in Asian medicine as an antiallergic drug. In the present study, the efficacy of the n-butanol extract of the seeds of the plant (MONB) is examined against ovalbumin-induced airway inflammation in guinea pigs. The test drugs (MONB or dexamethasone) are administered orally prior to challenge with aerosolized 0.5% ovalbumin. During the experimental period, bronchoconstriction tests are performed, and lung function parameters are measured. The blood and bronchoalveolar lavage fluid are collected to assess cellular content, and serum is used for cytokine (tumor necrosis factor-alpha, interleukin-4, and interleukin-6) assays. Histamine assays of lung tissue are performed using lung tissue homogenate. The results suggest that in ovalbumin-sensitized model control animals, tidal volume is decreased, respiration rate is increased, and both the total and differential cell counts in blood and bronchoalveolar lavage fluid are increased significantly compared with nonsensitized controls. MONB treatment shows improvement in all parameters except bronchoalveolar lavage tumor necrosis factor-alpha and interleukin-4. Moreover, MONB treatment demonstrates protection against acetylcholine-induced bronchoconstriction and airway inflammation. These results indicate that MONB has an inhibitory effect on airway inflammation. Thus, MONB possesses an antiasthmatic property through modulation of the relationship between Th1/Th2 cytokine imbalances. PMID:19966143

Mahajan, Shailaja G; Banerjee, Aryamitra; Chauhan, Bhupendrasinh F; Padh, Harish; Nivsarkar, Manish; Mehta, Anita A

2009-01-01

216

Evaluation of the polyphenol composition and antioxidant activity of African variety of Dacryodes edulis (G.Don) H.J Lam fruit.  

PubMed

Polyphenols are abundant micronutrients in our diet that have been credited with chemoprevention of diseases associated with oxidative stress. In this study, we investigated the whole ripened fruit of Dacryodes edulis (G.Don) H.J Lam, a multipurpose tree growing in West and Central Africa and other countries bordering the Gulf of Guinea, for polyphenol content as well as its antioxidant/radical scavenging capacity. Analysis of the methanol extract of the fruit by high-performance liquid chromatography coupled to an ultraviolet dual-array detector and mass-selective detector revealed the presence of catechol (9.27 mg/kg), gallate (10.40 mg/kg), methylgallate (0.88 mg/kg), ellagic acid (3.10 mg/kg), quercetin (0.21 mg/kg), and quercetin rhamnoside (0.76 mg/kg). The extract showed very high antioxidant potential (50% inhibitory concentration [IC(50)] = 14 microL), but a rather weak radical scavenging activity (IC(50) = 357 microL), when tested in vitro with the xanthine oxidase and 2-deoxyguanosine assay model systems, respectively. These results suggest that consumption of D. edulis could contribute to prevention of diseases that are related to oxidative stress. PMID:20041788

Atawodi, S E; Atawodi, J C; Idakwo, P; Pfundstein, B; Haubner, R; Wurtele, G; Spiegelhalder, B; Bartsch, H; Owen, R W

2009-12-01

217

Flavonols and anthocyanins of bush butter, Dacryodes edulis (G. Don) H.J. Lam, fruit. changes in their composition during ripening.  

PubMed

Dacryodes edulis is a multipurpose tree presently undergoing domestication in central Africa and the countries bordering the Gulf of Guinea, the fruits of which are a good source of essential fatty acids. Polyphenols were characterized in the skin zone and in the pulp of bush butter [Dacryodes edulis (G. Don) H.J. Lam] fruits at different stages of ripeness, from unripe to soft fruits. Total polyphenols, assayed according to the Folin-Ciocalteu method, accounted for 3.0-4.2 mg/g of fresh skin, corresponding to 18.6-21.6 mg/g of defatted dry skin, higher concentrations than in the pulp, with 1.1-1.4 mg/g of fresh weight, corresponding to 5.4-12.3 mg/g of defatted dry weight. Reversed-phase high-performance liquid chromatography revealed the presence of flavonols and anthocyanins. Flavonols appeared to be the main class, with quercitrin as the main individual compound, and the highest concentrations occurred in the skin zone. Hyperin, isoquercitrin, isorhamnetin rhamnoside, and isorhamnetin hexoside were also present in relatively high amounts. Petunidin, cyanidin, and peonidin hexosides were identified by mass spectrometry. In the course of ripening, the total polyphenols as well as the major flavonols increased slightly between unripe and preripe stages and then declined gradually as ripening progressed. Anthocyanin profiles also showed a substantial change during ripening, concomitant with the color change from pink to purple. PMID:14640602

Ella Missang, Crepin; Guyot, Sylvain; Renard, Catherine M G C

2003-12-01

218

Validation of turbulence and convective schemes on western Africa; comparison of LAM and CRM simulations on a HAPEX-Sahel case study  

NASA Astrophysics Data System (ADS)

The aim of this work is to analyse the behaviour of turbulence and convective parameterizations included in the Météo-France ALADIN-CLIMAT Limited Area Model in the frame of a 24 hour simulation of a HAPEX-Sahel case study, in comparison to observations and to a CRM (Méso-NH, with a 5 km horizontal grid-mesh) simulation carried out under the same boundary forcings. This framework provides an intermediate step of parameterization validation between the Single Column Model and Global Climate Model simulation studies. The chosen case study is the 21st August 1992 over a 12° x 12° region centred over SW Niger. It is characterised by the life cycle of a westward propagating convective system associated to an African Easterly Wave. Both LAM and CRM simulations have been performed over the same considered domain, using the same ERA40 boundary forcings. Sensitivity tests to resolution (both horizontal and vertical) have been first carried out with ALADIN-CLIMAT. Second, the two different convection schemes used in ALADIN-CLIMAT show two kinds of response mainly due to their different formulations of triggering (no constraints in the dry layer under the convective cloud versus continuous treatment of convection including this dry layer) and closure (moisture convergence versus CAPE). Third, the impact of entrainment at the top of the PBL (included in the turbulence scheme) and of convective downdrafts will be shown. Fourth, the impact of different boundary forcing fields will also be presented.

Pollack, D.; Gueremy, J. F.; Beau, I.

2009-04-01

219

Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts  

PubMed Central

Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro???-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36 ± 0.01 and 1.83 ± 0.03?mg/mL and IC90 values of 2.12 ± 0.04 and 2.62 ± 0.09?mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47 ± 0.006 and 0.71 ± 0.006?mg/mL, respectively. PMID:24558335

Mojarrab, Mahdi; Shiravand, Ali; Delazar, Abbas

2014-01-01

220

In Vivo and In Vitro Immunomodulatory Potential of Swertiamarin Isolated from Enicostema axillare (Lam.) A. Raynal That Acts as an Anti-inflammatory Agent.  

PubMed

Swertiamarin is a secoiridoid glycoside found in Enicostema axillare (Lam) A. Raynal, a medicinal plant used as a depurative in the Indian system of traditional medicine. The present study evaluated the immunomodulatory activity of isolated swertiamarin. In vivo immunomodulatory activity of swertiamarin (2, 5, and 10 mg/kg b.w.) was evaluated in a model of sheep red blood cells (SRBC) by assessing its effect on organ weight, hemagglutinating antibody titer (HA), plaque-forming cells (PFC), quantitative hemolysis of SRBC, and delayed type hypersensitivity (DTH). In vitro immunomodulatory potential was studied on isolated splenocytes, neutrophils, and peritoneal macrophages. In silico immunomodulatory effects were evaluated by docking of swertiamarin on proinflammatory cytokines to confirm its potential. In in vivo studies, the animals treated with swertiamarin showed a significant (P???0.05) increase in antibody titer, plaque-forming cells, and also in weight of the thymus and spleen. A decreased response to DTH reaction was recorded with the treatment of swertiamarin. In in vitro studies, treatment with swertiamarin modulated the messenger RNA (mRNA) and protein expression of IFN-?, IL-10, and IL-4 significantly (P???0.05) and also favored Th2-mediated response on concanavalin A (Con A)-induced splenocytes. The compound inhibited the release of free radicals significantly (P???0.05) in phytohemagglutinin (PHA)-induced neutrophils and also ameliorated the mRNA and protein expression of proinflammatory cytokines (TNF-?, IL-1?, and IL-6) in lipopolysaccharide (LPS)-induced macrophages. In in silico, the best docked pose of swertiamarin with the target proteins (TNF-?, IL-1?, and IL-6) was confirmed that swertiamarin acted as an anti-inflammatory mediator. PMID:24736879

Saravanan, S; Pandikumar, P; Babu, N Prakash; Islam, V I Hairul; Thirugnanasambantham, K; Paulraj, M Gabriel; Balakrishna, K; Ignacimuthu, S

2014-10-01

221

Anti-inflammatory and antioxidant effects of an ethanolic extract of the aerial parts of Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae).  

PubMed

Various parts of the perennial herb Hilleria latifolia (Lam.) H. Walt. (Family: Phytolaccaceae) are used in Ghanaian traditional medicine for the treatment of several inflammatory-related disorders. The present study examined the anti-inflammatory effect of an ethanolic extract of the aerial parts of Hilleria latifolia (HLE) in acute and chronic inflammation models. Since free radicals and reactive oxygen species are implicated in inflammatory diseases, the antioxidant potential of HLE was also investigated in in vitro experimental models. HLE (10-300 mg kg(-1), p.o.), either preemptively or curatively, significantly inhibited carrageenan-induced foot oedema in 7-day old chicks. Similarly, the NSAID diclofenac (10-100 mg kg(-1), i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg kg(-1), i.p.) dose-dependently reduced the oedema in both pre-emptive and curative treatments. In the Freund's adjuvant induced-arthritis model in rats, HLE as well as the positive controls, dexamethasone and methotrexate, showed significant anti-arthritic properties when applied to established adjuvant arthritis. HLE (10-300 mg kg(-1), p.o.) significantly reduced oedema in the ipsilateral paw of rats but failed to prevent systemic arthritic spread. The DMARD methotrexate (0.1-1 mg kg(-1), i.p.) and dexamethasone (0.3-3 mg kg(-1), i.p.) reduced significantly the total polyarthritic oedema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals. The extract (0.03-1.00 mg ml(-1)) exhibited Fe(3+) reducing activity, scavenged DPPH and prevented lipid peroxidation. These findings suggest that the extract exerts in vivo anti-inflammatory activity after oral administration and also has antioxidant properties which may contribute to its activity. PMID:23983331

Abotsi, W K M; Ainooson, G K; Woode, E

2012-01-01

222

Do Ecological Niche Model Predictions Reflect the Adaptive Landscape of Species?: A Test Using Myristica malabarica Lam., an Endemic Tree in the Western Ghats, India  

PubMed Central

Ecological niche models (ENM) have become a popular tool to define and predict the “ecological niche” of a species. An implicit assumption of the ENMs is that the predicted ecological niche of a species actually reflects the adaptive landscape of the species. Thus in sites predicted to be highly suitable, species would have maximum fitness compared to in sites predicted to be poorly suitable. As yet there are very few attempts to address this assumption. Here we evaluate this assumption. We used Bioclim (DIVA GIS version 7.3) and Maxent (version 3.3.2) to predict the habitat suitability of Myristica malabarica Lam., an economically important tree occurring in the Western Ghats, India. We located populations of the trees naturally occurring in different habitat suitability regimes (from highly suitable to poorly suitable) and evaluated them for their regeneration ability and genetic diversity. We also evaluated them for two plant functional traits, fluctuating asymmetry – an index of genetic homeostasis, and specific leaf weight – an index of primary productivity, often assumed to be good surrogates of fitness. We show a significant positive correlation between the predicted habitat quality and plant functional traits, regeneration index and genetic diversity of populations. Populations at sites predicted to be highly suitable had a higher regeneration and gene diversity compared to populations in sites predicted to be poor or unsuitable. Further, individuals in the highly suitable sites exhibited significantly less fluctuating asymmetry and significantly higher specific leaf weight compared to individuals in the poorly suitable habitats. These results for the first time provide an explicit test of the ENM with respect to the plant functional traits, regeneration ability and genetic diversity of populations along a habitat suitability gradient. We discuss the implication of these resultsfor designing viable species conservation and restoration programs. PMID:24312402

Nagaraju, Shivaprakash K.; Gudasalamani, Ravikanth; Barve, Narayani; Ghazoul, Jaboury; Narayanagowda, Ganeshaiah Kotiganahalli; Ramanan, Uma Shaanker

2013-01-01

223

Effects of Elevated CO2 and N Addition on Growth and N2 Fixation of a Legume Subshrub (Caragana microphylla Lam.) in Temperate Grassland in China  

PubMed Central

It is well demonstrated that the responses of plants to elevated atmospheric CO2 concentration are species-specific and dependent on environmental conditions. We investigated the responses of a subshrub legume species, Caragana microphylla Lam., to elevated CO2 and nitrogen (N) addition using open-top chambers in a semiarid temperate grassland in northern China for three years. Measured variables include leaf photosynthetic rate, shoot biomass, root biomass, symbiotic nitrogenase activity, and leaf N content. Symbiotic nitrogenase activity was determined by the C2H2 reduction method. Elevated CO2 enhanced photosynthesis and shoot biomass by 83% and 25%, respectively, and the enhancement of shoot biomass was significant only at a high N concentration. In addition, the photosynthetic capacity of C. microphylla did not show down-regulation under elevated CO2. Elevated CO2 had no significant effect on root biomass, symbiotic nitrogenase activity and leaf N content. Under elevated CO2, N addition stimulated photosynthesis and shoot biomass. By contrast, N addition strongly inhibited symbiotic nitrogenase activity and slightly increased leaf N content of C. microphylla under both CO2 levels, and had no significant effect on root biomass. The effect of elevated CO2 and N addition on C. microphylla did not show interannual variation, except for the effect of N addition on leaf N content. These results indicate that shoot growth of C. microphylla is more sensitive to elevated CO2 than is root growth. The stimulation of shoot growth of C. microphylla under elevated CO2 or N addition is not associated with changes in N2-fixation. Additionally, elevated CO2 and N addition interacted to affect shoot growth of C. microphylla with a stimulatory effect occurring only under combination of these two factors. PMID:22046376

Zhang, Lin; Wu, Dongxiu; Shi, Huiqiu; Zhang, Canjuan; Zhan, Xiaoyun; Zhou, Shuangxi

2011-01-01

224

Phosphorus status and microbial community of paddy soil with the growth of annual ryegrass (Lolium multiflorum Lam.) under different phosphorus fertilizer treatments*  

PubMed Central

Annual ryegrass (Lolium multiflorum Lam.) was grown in paddy soil in pots under different phosphorus (P) fertilizer treatments to investigate changes of P fractions and microbial community of the soil. The treatments included Kunyang phosphate rock (KPR) applications at 50 mg P/kg (KPR50) and 250 mg P/kg (KPR250), mono-calcium phosphate (MCP) application at 50 mg P/kg (MCP50), and the control without P application. The results showed that KPR50, KPR250, and MCP50 applications significantly increased the dry weight of the ryegrass by 13%, 38%, and 55%, and increased P uptake by 19%, 135%, and 324%, respectively. Compared with MCP50, the relative effectiveness of KPR50 and KPR250 treatments in ryegrass production was about 23% and 68%, respectively. After one season of ryegrass growth, the KPR50, KPR250, and MCP50 applications increased soil-available P by 13.4%, 26.8%, and 55.2%, respectively. More than 80% of the applied KPR-P remained as HCl-P fraction in the soil. Phospholipid fatty acid (PLFA) analysis showed that the total and bacterial PLFAs were significantly higher in the soils with KPR250 and MCP50 treatments compared with KPR50 and control. The latter had no significant difference in the total or bacterial PLFAs. The KPR50, KPR250, and MCP50 treatments increased fungal PLFA by 69%, 103%, and 69%, respectively. Both the principal component analysis and the cluster analysis of the PLFA data suggest that P treatments altered the microbial community composition of the soils, and that P availability might be an important contributor to the changes in the microbial community structure during the ryegrass growth in the paddy soils. PMID:19817001

Guo, Hai-chao; Wang, Guang-huo

2009-01-01

225

In vitro analysis on bactericidal screening and antioxidant potentiality of leaf and root extracts of Thottea siliquosa (Lam.) Ding Hou. An ethnobotanical plant  

PubMed Central

Objective Natural products of plant origin are potential source of novel antimicrobial and antioxidative agents. Thottea siliquosa (Lam.) Ding Hou. (T. siliquosa). A medicinal herb used by local tribals for treating various ailments. The present study aims at the phytochemical screening, GC-MS analysis, in vitro antibacterial activity and antioxidant potentiality of root and leaf extracts of T. siliquosa. Methods Hot continuous Soxhlet extraction, GC-MS analysis, antibacterial analysis by disc diffusion, microdilution assay and antioxidant potentialities by hydroxyl radical and nitric oxide radical scavenging. The data was statistically analyzed. Results Phytochemical screening of the ethyl acetate and methanolic extract of leaf and root revealed the presence of phenols, alkaloids, tannins and saponin. The extract revealed a pool of phytochemicals by comparison with authentic standards from spectral library. Both the extracts has shown their broad spectrum of inhibition against the selected bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia compared with standard antibiotic drug streptomycin. The extracts showed antioxidant activity by scavenging of free radicals such as hydroxyl and nitric oxide. The IC50 values of the ethyl acetate extracts leaf and root and standard in this assay were 167.5±0.67, 99.4±1.2, 192±2.5 µg/mL respectively. Similarly those methanolic extracts of leaf and root were 269.5±0.89 and 289.1±2.66 µg/mL respectively. Similarly, ethyl acetate and methanolic extracts also caused a moderate dose-dependent inhibition of nitric oxide with an IC50 range 65.5±1.55 to 148 ±3.09 µg/mL. The inhibitory activities were found to be dose dependent. Conclusion The present study provides evidence that ethyl acetate and methanol extract of leaf and root of T. siliquosa are potential source of natural antioxidants and bactericidal nature. It is essential that research should continue to isolate and purify the bio active components of this natural plant and use in drug discovery and development.

Nusaiba, Saiba Abdul Wahab; Murugan, Kumaraswamy

2013-01-01

226

Jennifer Lam's Research Reflection Paper  

E-print Network

and dirty rundown of the entire paper. The introduction, well, introduces the paper not to think about how your lack of textbook- reading stamina. Despair at how-fry yourself trying to understand this foreign language. Briefly consider asking

Adams, Mark

227

Construction of stable LamB-Shiga toxin B subunit hybrids: analysis of expression in Salmonella typhimurium aroA strains and stimulation of B subunit-specific mucosal and serum antibody responses.  

PubMed Central

The complete Shiga toxin B subunit and two N-terminal segments of the B subunit have been inserted into a cell surface exposed loop of the LamB protein, and expression of the hybrid proteins from three different promoter systems, i.e., (i) an in vitro-inducible tac promoter that provides high-level expression, (ii) the iron-regulated aerobactin promoter presumably induced in vivo under the iron-limiting conditions of the intestinal mucosal environment, and (iii) a synthetic, modified beta-lactamase promoter providing moderate level constitutive expression, has been analyzed in Escherichia coli, Salmonella typhimurium, and attenuated antigen carrier strains of S. typhimurium (aroA mutants). The hybrid vaccine strains were used to immunize mice by the oral and intraperitoneal routes. S. typhimurium aroA mutants apparently have a membrane export defect which prevents the transport of LamB and its derivatives across the cytoplasmic membrane. High-level expression of hybrid proteins through use of the tac promoter proved deleterious to the vaccine strains and prevented the production of viable cells at reasonable cell densities. The lower levels of gene expression observed with the beta-lactamase and aerobactin promoters did not have this effect. Immunization of mice with S. typhimurium aroA strains carrying the hybrid genes expressed from these two promoters resulted in significant B subunit-specific mucosal and serum antibody responses. This suggests that such expression systems may be useful when incorporated into candidate antidysentery live oral vaccines for inducing protection against the effect of Shiga toxin in infections caused by Shigella dysenteriae 1 and other Shiga toxin-or Shiga-like toxin-producing pathogens. Images PMID:1639503

Su, G F; Brahmbhatt, H N; Wehland, J; Rohde, M; Timmis, K N

1992-01-01

228

Combined U-Pb geochronology and Lu-Hf isotope systematics by LAM-ICPMS of zircons from granites and metasedimentary rocks of Carrazeda de Ansiães and Sabugal areas, Portugal, to constrain granite sources  

NASA Astrophysics Data System (ADS)

The Carrazeda de Ansiães and Sabugal areas in the Central Iberian Zone of the Iberian Variscan Belt contain abundant S-type granites formed during the post-thickening stage of the Variscan orogeny (syn- to post-D3, i.e. the last ductile deformation phase). Magmatic zircons and inherited zircon cores from two granites (G and G5), magmatic zircons from three other granites (G1, G4 and G6) and detrital zircons from a chlorite phyllite and a metagraywacke were dated by LAM-ICPMS. Hf isotope data were obtained by the same method to constrain the sources for granites. Some granites have zircons with negative ?Hft values (G: from - 8 to - 4; G1: from - 8 to - 1; G4: from - 6 to - 3), suggesting an origin from heterogeneous crustal anatectic melts or, from mantle-derived magmas contaminated in the crust. Magmatic zircons from granites G5 and G6 show a larger variation, yielding also some positive ?Hft values (G5: from - 8 to + 6; G6: from - 4 to + 4) indicating crystallization from mildly contaminated mantle-derived magmas or from a lower crustal source. U-Pb isotope data of inherited zircon cores from granites G and G5 show that Neoproterozoic ages dominate, but only the inherited zircon cores from granite G have similar initial 176Hf/177Hf ratios to those from a chlorite phyllite of the Formation of Ervedosa do Douro (Carrazeda de Ansiães area), suggesting that this metasedimentary unit could have been involved in the genesis of the granite G. On the other hand, the Lu-Hf data suggest that the metagraywacke from Sabugal area should not be considered a component involved in the partial melting process that formed the granite G5. The occurrence of Mesoproterozoic detrital zircons in both metasediments suggests that these rocks are likely to have evolved from a peri-Amazonian realm, close to West Avalonian terranes, during the Neoproterozoic/Early Palaeozoic. However, a West African Craton provenance could also be considered, if a long distance river transportation of Mesoproterozoic zircons, from the southwest Baltica or Arabian-Nubian Shield, is admitted. The U-Pb isotope data obtained in detrital zircons from both metasedimentary rocks suggest that their maximum depositional ages are ca. 578 Ma for the chlorite phyllite and ca. 577 Ma for the metagraywacke.

Teixeira, R. J. S.; Neiva, A. M. R.; Silva, P. B.; Gomes, M. E. P.; Andersen, T.; Ramos, J. M. F.

2011-07-01

229

Tetraploid Italian ryegrass (Lolium multiflorum Lam.)  

Microsoft Academic Search

1.Some preliminary experiments are described with C2- and C3-generations of tetraploid Italian ryegrass. In all cases the tetraploids had a lower dry matter content than the diploids.2.A C3-generation produced by mass selection equalled or surpassed the diploid mother strains in dry matter yield in some small experiments on several soil types in the first year. The tetraploids, however, were less

F. Wit

1958-01-01

230

Copyright 2011 Jennifer Lam-Anh Gosselin  

E-print Network

-of-river and barged hatchery spring/summer Chinook salmon (O. tshawytscha) collected immediately after passage through Survival: Examples from a model species (Poecilia reticulata) and salmonid species (Oncorhynchus spp __________________________________ #12;University of Washington Abstract Cumulative Experiences and Heterogeneity Affect Fish Survival

Washington at Seattle, University of

231

Less is More Chi Hin Lam (Tim)  

E-print Network

files don't really talk to each other Ease of Sharing Reproducible research? Meta-analysis Life-So-Obvious: Perhaps the way of we report our data in literature Data archeology? #12;4. Data Integration · The era of the "-omics" · Genomics. Metagenomics. · Tagomics? A-T-C-G A-T-C-G A-B-Z-0-1-2-3 A-T-C-G A-B-Z-0-1-2-3 #12

Hawai'i at Manoa, University of

232

Spore morphology of New Zealand Azolla filiculoides Lam. (Salviniaceae)  

Microsoft Academic Search

Sporocarps, megaspores, and micro-spores of Azolla filiculoides are described from fresh fertile material from the South Island of New Zealand. Size estimates are given along with light and scanning electron microscope photographs. A brief discussion of spore morphology, perine substructure, and leaf trichomes is given with regard to the taxonomy of A. filiculoides and A. rubra. Differences between these taxa

M. F. Large; J. E. Braggins

1993-01-01

233

Ecology and Management of Oxeye Daisy (Leucanthemum vulgare Lam.)  

Microsoft Academic Search

Oxeye daisy is a European perennial forb that spreads by seed and creeping rhizomes. Its distinctive flowerhead with yellow disc and white ray flowers and its spatula-shaped, lobed rosette leaves help identify this Montana Category I noxious weed. First recorded from Lewis and Clark County in 1890, it spread to 24 counties by 2007. Oxeye daisy is a prolific seed

Jim Jacobs

234

Life Science Fall 2011 110901 Eric Lam (Imperial College, London)  

E-print Network

-FOXM1 axis in cancer and drug resistance" Host: Marek Los, IGEN 110908 Hal Hoffman (UCSD to killing by apoptin; therapeutic perspectives" Host: Marek Los, IGEN 111013 Hjalmar Brismar (KI, KTH of infectious diseases ­ Toward point-of-care instruments " Host: Maria Lerm 111028 Ola Söderberg (Associate

Zhao, Yuxiao

235

An Exploration of Distributed Social Networking Monica S. Lam  

E-print Network

that owns our personal data in the cloud, and discusses ramifications of loss of privacy and fair-market to share their GPS location with friends, the web is supplanting the classic Personal Computing paradigm and configure your personal data? They make it easy to share. If you and your friends use the same site

Pratt, Vaughan

236

Antiasthmatic activity of Moringa oleifera Lam: A clinical study.  

PubMed

The present study was carried out to investigate the efficacy and safety of seed kernels of Moringa oleifera in the treatment of bronchial asthma. Twenty patients of either sex with mild-to-moderate asthma were given finely powdered dried seed kernels in dose of 3 g for 3 weeks. The clinical efficacy with respect to symptoms and respiratory functions were assessed using a spirometer prior to and at the end of the treatment. Hematological parameters were not changed markedly by treatment with M. oleifera. However, the majority of patients showed a significant increase in hemoglobin (Hb) values and Erythrocyte sedimentation rate (ESR) was significantly reduced. Significant improvement was also observed in symptom score and severity of asthmatic attacks. Treatment with the drug for 3 weeks produced significant improvement in forced vital capacity, forced expiratory volume in one second, and peak expiratory flow rate values by 32.97 ± 6.03%, 30.05 ± 8.12%, and 32.09 ± 11.75%, respectively, in asthmatic subjects. Improvement was also observed in % predicted values. None of the patients showed any adverse effects with M. oleifera. The results of the present study suggest the usefulness of M. oleifera seed kernel in patients of bronchial asthma. PMID:21264158

Agrawal, Babita; Mehta, Anita

2008-01-01

237

Antimicrobial isothiocyanates from the seeds of Moringa oleifera Lam.  

PubMed

4-(alpha-L-Rhamnosyloxy)benzyl isothiocyanate (1) and 4-(4'-O-acetyl-alpha-L-rhamnosyloxy)-benzyl isothiocyanate (2) isolated from Moringa oleifera seeds were screened for their antibacterial activities against Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Pseudomonas aeruginosa, and for their antifungal activities against Candida albicans, Trichophyton rubrum, and Epidermophyton floccosum using the disk diffusion method. Isothiocyanates 1 and 2 were found active at the lowest inhibitory concentration of 1 mg/ml against all Gram-positive bacteria tested (S. aureus, S. epidermidis, B. subtilis) and against the dermatophytic fungi E. floccosum and T. rubrum. Statistically significant differences were found between the mean inhibition zones (IZ) of 1 and 2 and the standard drugs, ofloxacin and clotrimazole. The minimum inhibitory concentration (MIC) values confirmed the good antimicrobial activity of 1 and 2 against S. aureus, good to moderate activity against S. epidermidis, moderate activity against B. subtilis, and weak activity against E. floccosum and T. rubrum. The in vitro bactericidal effect of 1 and 2 against the Gram-positive bacterial strains tested is suggested by MBC:MIC ratios of 2:1. PMID:23413749

Padla, Eleanor P; Solis, Ludivina T; Levida, Ruel M; Shen, Chien-Chang; Ragasa, Consolacion Y

2012-01-01

238

Neural Network Models for Antony Lam Amar Kaheja  

E-print Network

Govindaraj School of Textiles and Materials Technology Philadelphia University Philadelphia, PA, 19144 E of fabric properties for the apparel textile industry and some industrial textile applications. This unique and production time will shrink. Drape prediction has been one of the biggest challenges to the textile industry

Raheja, Amar

239

Evaluation of free radical scavenging activity of Butea monosperma Lam.  

PubMed

In the present study, ethyl acetate, butanol and aqueous fractions derived from total methanol extract of Butea monosperma flowers were evaluated for radical scavenging activities using different in vitro models like reducing power assay, scavenging of 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and inhibition of erythrocyte hemolysis using 2, 2' azo-bis (amidinopropane) dihydrochloride (AAPH). Methanol extract along with its ethyl acetate and butanol fractions showed potent free radical scavenging activity, whereas aqueous fraction was found to be devoid of any radical scavenging properties. The observed activity could be due to the higher phenolic content in the extracts (16.1, 25.29, and 17.74% w/w in methanol extract, ethyl acetate and butanol fractions respectively). HPTLC fingerprint profile of the ethyl acetate and butanol fractions were developed which would serve as reference standard for quality control of the extracts. PMID:17477311

Lavhale, Manish S; Mishra, S H

2007-04-01

240

Evaluation of free radical scavenging activity of Butea monosperma Lam  

Microsoft Academic Search

In the present study, ethyl acetate, butanol and aqueous fractions derived from total methanol extract of Butea monosperma flowers were evaluated for radical scavenging activities using different in vitro models like reducing power assay, scavenging of 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and inhibition of erythrocyte hemolysis using 2, 2' azo-bis (amidinopropane) dihydrochloride (AAPH).

Manish S Lavhale; S H Mishra

2007-01-01

241

Baseline Mechanical Characterization of J774 Macrophages Jonathan Lam,  

E-print Network

's modified Eagle's medium sup- plemented with 10% fetal bovine serum (FBS) at 37 C with 5% CO2 to lymphocytes. Due to this multitasking aptitude, macrophage cell lines like J774 or Raw 264.7 are well membrane reserves during autonomous deformation. MATERIALS AND METHODS J774 macrophage cell line Mouse

Heinrich, Volkmar

242

Phytotoxic compounds from roots of Centaurea diffusa Lam.  

PubMed Central

An extract of roots of Centaurea diffusa (diffuse knapweed) yielded caryophyllene oxide and linoleic acid which were shown to be phytotoxic. Also isolated were germacrene B, a previously-known phytotoxin as well as the inactive polyene aplotaxene. A combination of these compounds, if transferred to the soil, could be one factor in the invasive behavior of this weed. Contrary to a literature report, 8-hydroxyquinoline was not detected in root exudates of in vitro grown C. diffusa nor could it be identified in the root extract. However, a recent report from a different group maintains that 8-hydroxyquinoline can be released from roots of C. diffusa following a diurnal rhythm. PMID:19568334

Quintana, Naira; El Kassis, Elie G; Stermitz, Frank R

2009-01-01

243

M. Benosman LAM, Universite de Reims Champagne-Ardenne,  

E-print Network

with the constraints of eliminating or at least minimizing the residual elastic deflection of the end-effector. The last problem is more challenging and con- cerns the tracking of desired end-effector operational , a method based on control input shaping is presented on spring-mass oscillating systems. The method called

De Luca, Alessandro

244

Medicinal potential of Morella serata (Lam.) Killick (Myricaceae) root extracts: biological and pharmacological activities  

PubMed Central

Background Morella serata is a South African medicinal plant used in the treatment of microbial infections and to enhance male sexual performance. There is dearth of information in scientific literature on its efficacy and safety. Methods In the present study, the root extracts were investigated for the phytochemicals that may be present the antibacterial, anticandida activity using 96 wells microtitre plate method and cytotoxicity using brine shrimp (Artemia salina) lethality assay. Results The qualitative phytochemical screening revealed the presence of tannins, saponins, flavonoids, terpenoids and steroids. All the extracts including water inhibited both Gram positive and Gram negative bacteria strains at minimum inhibitory concentrations (MIC) ranging from 0.09 – 6.25 mgmL-1. The best activity was observed in the acetone extract inhibiting all the bacteria tested at MIC range of 0.09 – 0.78 mgmL-1 except Shigella flexneri KZN that was inhibited at 1.56 mgmL-1. Similarly, all the extracts suppressed the growth of all Candida species and Trichophyton mucoides at MIC ranging from 0.13 – 3.13 mgmL-1. The cytotoxicity assay revealed potent cytotoxic potential of M. serata methanol and ethanol root extracts by displaying LC50 of 0.26 and 0.18 ?gmL-1 respectively. Conclusion The results obtained from the present study indicated broad spectrum antimicrobial activity and justifies the use of the plant in the treatment of infectious diseases. Also the species could be a good natural source of antitumor compounds considering its lethality against brine shrimp nauplii. PMID:23829770

2013-01-01

245

Nutritional value of aquatic ferns ( Azolla filiculoides Lam. and Salvinia molesta Mitchell) in pigs  

Microsoft Academic Search

Aquatic ferns (AFs) such as Azolla filiculoides and Salvinia molesta are grown on swine lagoons in the tropics and used to feed pigs. The present work aimed to determine their nutritional value. Azolla samples collected in Colombian pig farms contained from 184 to 317g crude protein\\/kg DM, 469–620g NDF and 98–145g ash. Those of Salvinia contained from 112 to 169g

Pascal Leterme; Angela M. Londoño; Jaime E. Muñoz; Jeimmy Súarez; Carlos A. Bedoya; Wolfgang B. Souffrant; André Buldgen

2009-01-01

246

Quantitative and qualitative DNA variations in Anabaena azollae Strasb. living in Azolla filiculoides lam.  

PubMed

Heterocysts and vegetative cells of the filamentous nitrogen-fixing Anabaena azollae isolated from the apex to the basal leaf cavities of Azolla filiculoides were examined by epifluorescent microscope after fluorochrome staining. Acridine orange (AO), DAPI, and chromomycin fluorochromes were used in order to evidence total DNA content and respectively, A + T and G + C bases. Measurements of fluorescence intensities were made on photographic prints by the automatic image analysis system Quantimet 970. Heterocysts contained higher amounts of DNA than did vegetative cells, and their content strongly increased in the basal leaf cavities. The heterocyst DAPI brightness was quite uniform, whereas in vegetative cells DAPI brightness increased from the apex to the basal groups. In vegetative cells from the apex to the median group, the percentage of DAPI brightness was 60-85% with respect to AO brightness, whereas in heterocysts of the same groups DAPI brightness was 40-50% with respect to AO brightness. In the basal group, brightness due to DAPI staining was comparable with those of previous group both in heterocysts and in vegetative cells, whereas chromomycin brightness increased strongly in heterocysts. These data show that heterocyst changes its DNA content and composition in the basal leaf cavities, suggesting that its lifetime is not completely over. PMID:1374307

Canini, A; Grilli Caiola, M; Ferrucci, L

1992-01-01

247

Nutritional value and intake of aquatic ferns ( Azolla filiculoides Lam. and Salvinia molesta Mitchell.) in sows  

Microsoft Academic Search

Aquatic ferns (AFs) such as Azolla filiculoides and Salvinia molesta are grown on swine lagoons in the tropics and used in diets for pigs. The present work is aimed at evaluating their potential as feed ingredients for sows. When presented with ad libitum AFs, gilts weighing 110±14kg (mean±SD), were able to ingest 9.1–9.7kg fresh AF per day (from 597 to

Pascal Leterme; Angela M. Londoño; Diana C. Ordoñez; Alejandra Rosales; Fernando Estrada; Jérôme Bindelle; André Buldgen

2010-01-01

248

Differential responses of Azolla microphylla Kaulf and Azolla filiculoides Lam. to lead nitrate  

Microsoft Academic Search

The aquatic fernAzolla is used as a green manure for rice production systems in warm temperate and tropical regions throughout the world. We used\\u000a lead nitrate in nutrient media to assess the tolerance\\/response of twoAzolla microphylla andAzolla filiculoides to heavy metals. While both species showed negative responses in growth with an increase in lead concentrations, They were\\u000a distinctly different in

Wamy Eyini Muthukumaras; Natesan Anjana Devi; Chinnathambi Pothiraj; Muthukrishnan Jayakumar; Bong-Seop Kil

2000-01-01

249

Differential response of Azolla microphylla kaulf. and Azolla filiculoides Lam. to sodium fluoride  

Microsoft Academic Search

We assessed the differential response ofAzolla microphylla andAzolla filiculoides to fluoride stress by growing them in culture media containing 1 to 50 ppm sodium fluoride (NaF).A microphylla had a higher total chlorophyll content thanA. filiculoides. Both species showed gradual decreases in protein content as the concentration of NaF increased.A. microphylla accumulated less proline thanA. filiculoides when more NaF was added

M. Eyini; K. Sujanandini; C. Pothiraj; M. Jayakumar; Bong-Seop Kil

1999-01-01

250

78 FR 3319 - Amendments to Existing Validated End User Authorizations: Advanced Micro Devices China, Inc., Lam...  

Federal Register 2010, 2011, 2012, 2013

...Clarification of Scope of Entries in Supplement AGENCY: Bureau of Industry and Security...PRC). Specifically, BIS amends Supplement No. 7 to part 748 of the EAR to update...Wuxi) Ltd. Finally, BIS amends Supplement No. 7 to part 748 of the EAR to...

2013-01-16

251

Factors affecting postharvest preservation of safou (Dacryodes edulis (G. Don) H.J. Lam) fruits  

Microsoft Academic Search

The effect of five factors including fruit size, ripeness stage, picking mode, packaging mode, and storage temperature on safou softening was investigated. Temperature, packaging mode, and fruit size were the only factors that significantly affected safou softening during storage. Low temperature (18 °C) associated with storage without packaging or with perforated packaging improved safou postharvest shelf life. Under these conditions,

Bayi Reine Dossou; Crépin Ella Missang; Alain Baron; Catherine M. G. C. Renard; Thomas Silou

2012-01-01

252

Feeding preferences of the collembolan Onychiurus sinensis for fungi colonizing holm oak litter (Quercus rotundifolia Lam.)  

E-print Network

of our test fungi, but not on the Basidiomycete S41 and on Trichoderma polysporum. Collembola / feeding reproduire, à l'exception du Basidiomycète S41 et de Trichoderma polysporum. Collemboles/ préférences

Paris-Sud XI, Université de

253

Diffuse knapweed ( Centaurea diffusa Lam.) seedling emergence and establishment in a Colorado grassland  

Microsoft Academic Search

Knapweeds (Centaurea spp.) are damaging invaders of grasslands and other North American rangelands. A field study was conducted to determine conditions\\u000a that promote diffuse knapweed (C. diffusa) emergence and establishment in a native Colorado grassland (North America). Knapweed was planted in native grassland under\\u000a treatments with different opening sizes, levels of competition, knapweed seed burial and season of seeding. There was

Paul J. Meiman; Edward F. Redente; Mark W. Paschke

254

Diffuse knapweed ( Centaurea diffusa Lam.) seedling emergence and establishment in a Colorado grassland  

Microsoft Academic Search

Knapweeds (Centaurea spp.) are damaging invaders of grasslands and other North American rangelands. A field study was conducted to determine conditions\\u000a that promote diffuse knapweed (C. diffusa) emergence and establishment in a native Colorado grassland (North America). Knapweed was planted in native grassland under\\u000a treatments with different opening sizes, levels of competition, knapweed seed burial and season of seeding. There was

Paul J. Meiman; Edward F. Redente; Mark W. Paschke

2009-01-01

255

Fine structure of the axial complex of Sphaerechinus granularis (Lam.) (Echinodermata: Echinoidea)  

Microsoft Academic Search

Three regions of the axial complex in Sphaerechinus granularis can be distinguished: 1) The axial organ which protrudes from one side of the axial sinus; the sinus septum which separates the sinus from the body cavity and encloses the stone canal; the pulsating vessel which runs along the inside of the axial organ. 2) The blindly-ending terminal sinus in which

Sebastian Bachmann; Alfred Goldschmid

1978-01-01

256

Design of a VIA Based Communication Protocol for LAM\\/MPI Suite  

Microsoft Academic Search

The increasing use of System Area Network (SAN) de- mands efficient communication to benefit of SAN features through a direct access to network resources and avoiding kernel intervention in communication path. Recently, a consortium composed by Microsoft, Compaq and Intel authored a new standard, the Virtual Interface Ar- chitecture (VIA), designed to reduce software overhead in data transfers. This paper

Massimo Bertozzi; Marco Panella; Monica Reggiani

2001-01-01

257

Integration and test activities for the SUMIRE prime focus spectrograph at LAM  

NASA Astrophysics Data System (ADS)

The Prime Focus Spectrograph (PFS) of the Subaru Measurement of Images and Redshifts (SuMIRe) project for Subaru telescope consists in four identical spectrographs feed by 600 fibers each. Each spectrograph is composed by an optical entrance unit that creates a collimated beam and distributes the light to three channels, two visible and one near infrared. We present here the integration process of the first spectrograph channel. The verification requirements, the specific integration requirements and the product tree are the main drivers from the top plan for the Assembly Integration and Test (AIT) development process. We then present the AIT flow-down, the details for the AIT processes as well as opto-mechanical alignment procedures and tests setup. In parallel, we are developing and validating dedicated tools to secure and facilitate the AIT activities, as we have to assemble eight visible cameras, integrate and align four fiber slits, integrate and align the components of four spectrographs.

Madec, F.; Jaquet, Marc; Pascal, Sandrine; Bozier, A.; Le Mignant, David; Vives, S.; Ferrand, D.; Pegot-Ogier, T.; Arthaud, G.; Golebiowski, M.; Sugai, H.; Tamura, N.; Gunn, J.; Smee, S.; Oliveira, L.

2014-07-01

258

Antitrypanosomal Property of Some Extracts of Different Parts of Moringa oleifera, Lam  

Microsoft Academic Search

Different extracts of Moringa oleifera parts were evaluated for their anti-trypanosomal activity and broad phytochemical classes. Alkaloids, resins and saponins were detected. In vitro, the petroleum ether extract of the root bark, chloroform extract of the stem bark, methanol extracts of the stem and the aqueous extracts of all parts were active at 4 and 2 mg\\/ ml doses. The

Sunday E. Atawodi; Hassana Shehu

259

Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam  

PubMed

Moringa oleifera is an important multipurpose tree introduced to Africa from India at the turn of this century. Despite limited knowledge of the levels of genetic diversity and relatedness of introduced populations, their utilization as a source of seed for planting is widespread. In order to facilitate reasoned scientific decisions on its management and conservation and prepare for a selective breeding programme, genetic analysis of seven populations was performed using amplified fragment length polymorphism (AFLP) markers. The four pairs of AFLP primers (PstI/MseI) generated a total of 236 amplification products of which 157 (66.5%) were polymorphic between or within populations. Analysis of molecular variance (AMOVA) revealed significant differences between regions and populations, even though outcrossing perennial plants are expected to maintain most variation within populations. A phenetic tree illustrating relationships between populations suggested at least two sources of germplasm introductions of Kenya. The high levels of population differentiation detected suggest that provenance source is an important factor in the conservation and exploitation of M. oleifera genetic resources. PMID:10199008

Muluvi; Sprent; Soranzo; Provan; Odee; Folkard; McNicol; Powell

1999-03-01

260

Amelioration of ionizing radiation induced lipid peroxidation in mouse liver by Moringa oleifera Lam. leaf extract.  

PubMed

Protective effect of Moringa oleifera leaf extract (MoLE) against radiation-induced lipid peroxidation has been investigated. Swiss albino mice, selected from an inbred colony, were administered with MoLE (300 mg/kg body wt) for 15 days before exposing to a single dose of 5 Gy 60Co-gamma radiation. After treatments, animals were necropsied at different post irradiation intervals (days 1, 7 and 15) and hepatic lipid peroxidation and reduced glutathione (GSH) contents were estimated to observe the relative changes due to irradiation and its possible amelioration by MoLE. It was observed that, MoLE treatment restored GSH in liver and prevented radiation induced augmentation in hepatic lipid peroxidation. Phytochemical analysis showed that MoLE possess various phytochemicals such as ascorbic acid, phenolics (catechin, epicatechin, ferulic acid, ellagic acid, myricetin) etc., which may play the key role in prevention of hepatic lipid peroxidation by scavenging radiation induced free radicals. PMID:22439436

Sinha, Mahuya; Das, Dipesh Kr; Datta, Sanjukta; Ghosh, Santinath; Dey, Sanjit

2012-03-01

261

Preventive effects of Moringa oleifera (Lam) on hyperlipidemia and hepatocyte ultrastructural changes in iron deficient rats.  

PubMed

The effects of Moringa oleifera (MO), Moringaceae on hyperlipidemia and hepatocyte ultrastructural changes caused by iron deficiency were investigated. Four-week-old male Wistar-strain rats were fed a control diet based on AIN-93G (C), an iron deficient diet (FeD), a FeD + 0.5% MO (FeD-m) diet, or a FeD + MO 1% (FeD-M) diet for 4 weeks. It was found that MO reduced iron-deficient diet-induced increases in serum and hepatic lipids with dose-dependent increases of serum quercetin and kaempherol, but did not prevent anemia. By electron microscopy, in iron deficient hepatocytes, slightly swollen mitochondria and few glycogen granules were observed, but glycogen granules increased and mitochondria were normalized by treatment with MO. Furthermore, lipoproteins were observed in the Golgi complex under treatment with MO. These results suggest a possible beneficial effect of MO in the prevention of hyperlipidemia and ultrastructural changes in hepatocytes due to iron-deficiency. PMID:17690476

Ndong, Moussa; Uehara, Mariko; Katsumata, Shinichi; Sato, Shigeru; Suzuki, Kazuharu

2007-08-01

262

Untersuchungen zur Verbreitung von Indolglucosinolaten in höheren Pflanzen: Moringa oleifera Lam  

Microsoft Academic Search

Summary Moringa oleifera (Moringaceae, Rhoeadales) does not synthesize indoleglucosinolates. This is in contrast to all other mustard oil-containing families of the Rhoeadales (sensu Wettstein). Experimental proof was provided by the application of C14-indole, C14-tryptophan, and S35-sulphate. This lack of ability to synthesize glucobrassicin or neoglucobrassicin may be of taxonomic importance.

H. Schraudolf

1967-01-01

263

Bioremediation of turbid surface water using seed extract from Moringa oleifera Lam. (drumstick) tree.  

PubMed

An indigenous water treatment method uses Moringa oleifera seeds in the form of a water-soluble extract in suspension, resulting in an effective natural clarification agent for highly turbid and untreated pathogenic surface water. Efficient reduction (80.0% to 99.5%) of high turbidity produces an aesthetically clear supernatant, concurrently accompanied by 90.00% to 99.99% (1 to 4 log) bacterial reduction. Application of this low-cost Moringa oleifera protocol is recommended for simplified, point-of-use, low-risk water treatment where rural and peri-urban people living in extreme poverty are presently drinking highly turbid and microbiologically contaminated water. PMID:20131221

Lea, Michael

2010-02-01

264

Quality of cooked ground buffalo meat treated with the crude extracts of Moringa oleifera (Lam.) leaves.  

PubMed

The study was conducted to evaluate the physico-chemical, microbial and organoleptic qualities of cooked ground buffalo meat (GBM), treated with, 1, 1.5 and 2% levels of aqueous solution of crude extract of drumstick (Moringa oleifera) leaves. The meat samples treated with 1.5% crude extract of drumstick leaves significantly (P??0.05) difference was observed in juiciness, tenderness and overall acceptability scores between the treated meat samples. PMID:23572848

Hazra, Suchandra; Biswas, Subhasish; Bhattacharyya, Debasish; Das, Sudip Kumar; Khan, Anupam

2012-04-01

265

Evaluation of hematological and hepatorenal functions of methanolic extract of Moringa oleifera Lam. root treated mice.  

PubMed

Methanolic extract of M. oleifera root was found to contain some alkaloids (total alkaloid 0.2%). Effects of multiple weekly (35, 46, 70 mg/kg) and daily therapeutic (3.5, 4.6, 7.0 mg/kg) i.p. doses of the crude extract (CE) on liver and kidney functions and hematological parameters in mice were studied. No alteration in hematological and biochemical parameters at low and moderate dose level of daily and low dose level of weekly treatment of the extract was observed. However, the extract at moderate dose level in weekly treatment changed serum aminotransferase and plasma cholesterol levels significantly. High dose in addition to the above parameters changed total bilirubin, non protein nitrogen, blood urea and plasma protein. High dose of daily treatment and moderate and high dose of weekly treatment of CE increased WBC count and decreased clotting time significantly. The results indicate that the weekly moderate and high dose (> 46 mg/kg body wt.) and daily/therapeutic high dose (7 mg/kg) of CE affects liver and kidney functions and hematological parameters whereas the weekly dose (3.5 mg/kg) and low and moderate daily/therapeutic dose (3.5 and 4.6 mg/kg) did not produce adverse effects on liver and kidney functions. PMID:10641193

Mazumder, U K; Gupta, M; Chakrabarti, S; Pal, D

1999-06-01

266

Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds.  

PubMed

Seeds of the tropical tree Moringa oleifera contain small storage proteins able to flocculate particles in suspension in water. The cDNA encoding one of these flocculent proteins, MO(2.1), was cloned and the recombinant protein was expressed in Escherichia coli. The flocculent activity of the purified recombinant MO(2.1)was assayed on clays and bacteria using light and confocal microscopy and GFP-overexpressing bacteria. We show that MO(2.1)is able to aggregate montmorillonite clay particles as well as gram-positive and gram-negative bacteria. We discuss the use of recombinant proteins to study flocculating properties and improve water purification processes. PMID:12382051

Broin, M; Santaella, C; Cuine, S; Kokou, K; Peltier, G; Joët, T

2002-10-01

267

Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds  

Microsoft Academic Search

Seeds of the tropical tree Moringa oleifera contain small storage proteins able to flocculate particles in suspension in water. The cDNA encoding one of these flocculent proteins, MO2.1, was cloned and the recombinant protein was expressed in Escherichia coli. The flocculent activity of the purified recombinant MO2.1 was assayed on clays and bacteria using light and confocal microscopy and GFP-overexpressing

M. Broin; C. Santaella; S. Cuine; K. Kokou; G. Peltier; T. Joët

2002-01-01

268

Quality of cooked ground buffalo meat treated with the crude extracts of Moringa oleifera (Lam.) leaves  

Microsoft Academic Search

The study was conducted to evaluate the physico-chemical, microbial and organoleptic qualities of cooked ground buffalo meat\\u000a (GBM), treated with, 1, 1.5 and 2% levels of aqueous solution of crude extract of drumstick (Moringa oleifera) leaves. The\\u000a meat samples treated with 1.5% crude extract of drumstick leaves significantly (P?

Suchandra Hazra; Subhasish Biswas; Debasish Bhattacharyya; Sudip Kumar Das; Anupam Khan

269

Immunomodulatory effect of Moringa oleifera Lam. extract on cyclophosphamide induced toxicity in mice.  

PubMed

Immunomodulatory effect of ethanolic extract (50%) of M. oleifera leaves (MOE) has been studied in normal and immunosuppressed mice models. Different doses of MOE i.e. 125, 250 and 500 mg/kg body weight of mice were administered orally for 15 days. Cyclophosphamide at a dose of 30 mg/kg body weight was administered orally for the next 3 days. On day 16 and 19, hematological parameters like white blood cell (WBC) count, red blood cell (RBC) count, haemoglobin level (Hb), percent neutrophils and organ weight were recorded. Effect of MOE on phagocytic activity of mice macrophages was determined by carbon clearance test. MOE showed significant dose dependent increase in WBC, percent neutrophils, weight of thymus and spleen along with phagocytic index in normal and immunosuppressed mice. The results indicate that MOE significantly reduced cyclophosphamide induced immunosuppression by stimulating both cellular and humoral immunity. PMID:21117458

Gupta, Anamika; Gautam, Manish K; Singh, Rahul K; Kumar, M Vijay; Rao, Ch V; Goel, R K; Anupurba, Shampa

2010-11-01

270

Genetic diversity of Pogonatherum paniceum (Lam.) Hack. in Southwest China revealed by AFLP markers  

Microsoft Academic Search

species level. Observed number of alleles was 1.9093 ± 0.2875 and effective number of alleles was 1.4048 ± 0.3342. Nei's gene diversity and Shannon's Information index were 0.2494 ± 0.1657 and 0.3903 ± 0.2186, respectively. Nei's genetic distance among 22 populations ranged from 0.0996 to 0.6952. Genetic identity among populations ranged from 0.4990 to 0.9052, averaging 0.7387. Unweighted pair group

Guo-Qing Zhuang; Dan-Wei Ma; Wen-Guo Wang; Sheng-Hua Wang; Fang Chen

271

Clinical & Experimental Pathology Lamming, J Clin Exp Pathol 2012, S4  

E-print Network

in mammals, focusing particularly on resveratrol, rapamycin, and metformin. Resveratrol One of the most frequently mentioned potential anti-aging compounds is resveratrol, a polyphenol found in a variety of plant] showed using an in vitro assay that resveratrol and several structurally-related compounds activate SIRT1

Sabatini, David M.

272

Triterpenes and Steroids from Euphorbia denticulata Lam. With Anti-Herpes Symplex Virus Activity  

PubMed Central

In this research, dried acetone:chloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3?,25-diol, cycloart-23E-ene-3?,25- diol, ergosta-8,24-dien-3-ol (obtusifoliol) and beta-sitosterol which were reported for the first time from this plant. The structure of these compounds was elucidated by NMR and mass spectroscopic methods. The MTS assay was used to determine the toxicity and antiviral activity of betulin and (3?,23E)-cycloarta-23-ene-3,25-diol. Betulin showed anti-HSV-1 activity with EC50 value of 84.37±0.02 ?g/mL, and toxicity on normal vero cells with CC50 value of 660.718±0.072 ?g/mL. (3?,23E)-Cycloarta-23-ene-3,25-diol showed antiviral effect with EC50 value of 86.63±0.03 ?g/mL, and toxicity with CC50 value of 1089.21±0.25 ?g/mL. The results revealed that these two compounds have the antiviral activity far below the CC50 value with selectivity index (CC50/EC50) values of 7.83, and 12.57, respectively. PMID:24523756

Shamsabadipour, Sara; Ghanadian, Mustafa; Saeedi, Hojjatollah; Rahimnejad, Mohammad Reza; Mohammadi-Kamalabadi, Marzieh; Ayatollahi, Seyed Majid; Salimzadeh, Loghman

2013-01-01

273

UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kevin Petersen, On Tak Lam, Zhejian Zhang  

E-print Network

. With this consumption, there is the opportunity to remanufacture the used cartridges and reduce the carbon footprint figures in regards to the waste and the reduction the carbon footprint are not available due will on reduce the costs of obtaining cartridges from 10%-30% while also reducing the carbon footprint

274

Agrobacterium tumefaciens -mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.)  

Microsoft Academic Search

Agrobacterium tumefaciens strain LBA4404 carrying plasmid pTOK233 encoding the hygromycin resistance (hph) and #-glucuronidase (uidA) genes has been used to transform two agronomic grass species: tall fescue (Festuca arundinacea) and Italian ryegrass (Lolium multiflorum). Embryogenic cell suspension colonies or young embryogenic calli were co-cultured with Agrobacterium in the presence of acetosyringone. Colonies were grown under hygromycin selection with cefotaxime and

A. J. E. Bettany; S. Dalton; E. Timms; B. Manderyck; M. Dhanoa; P. Morris

2003-01-01

275

Anti-obese activity of Butea monosperma (Lam) bark extract in experimentally induced obese rats.  

PubMed

To study the efficacy of ethanolic extract of B. monosperma bark in cafeteria and atherogenic diet fed rats and monosodium glutamate (MSG) obese rats, different doses (200, 400 and 800 mg/kg) of ethanolic extract of B. monosperma bark showed dose dependent decrease in body weight, daily food intake, glucose, lipids, internal organs' weight and fat pad weight in cafeteria and atherogenic diet fed rats and monosodium glutamate obese rats. The results suggested that B. monosperma has significant anti-obese activity. PMID:22822527

Dixit, P; Prakash, T; Karki, Roopa; Kotresha, D

2012-07-01

276

Genetic diversity and population structure of Butea monosperma (Lam.) Taub.- a potential medicinal legume tree.  

PubMed

Three molecular marker systems, Random Amplified Polymorphic DNA (RAPD), Inter-Simple Sequence Repeats (ISSR) and Sequence-Related Amplified Polymorphism (SRAP) were employed to investigate the genetic structure and diversity among the 14 natural populations of Butea monosperma collected from different geographical regions of India. Detected by 17 RAPD, 15 ISSR and 11 SRAP primer combinations, the proportions of polymorphic bands were 84.2 %, 77.2 % and 91.9 %, respectively, and the mean Nei's genetic distances among the populations were 0.13, 0.10 and 0.13, respectively. Partitioning of genetic variability by Analysis of molecular variance (AMOVA) revealed that the high genetic diversity was distributed within the populations. AMOVA also revealed that the coefficient of gene differentiation among populations based on FST was very high irrespective of markers used. The overall gene flow among populations (Nm) was very low. Cophenetic correlation coefficients of Nei's distance values and clustering pattern by Mental test were statistically significant for all three marker systems used but poor fit for ISSR data than for RAPD, SRAP and combined data set of all three markers. For all markers, a high similarity in dendrogram topologies was obtained, although some differences were observed with ISSR. The dendrogram obtained by RAPD, SRAP and combined data set of all three markers reflect relationship of most of the populations according to their geographic distribution. PMID:24431507

Vashishtha, Amit; Jehan, Tabassum; Lakhanpaul, Suman

2013-07-01

277

Improved management of Butea monosperma (Lam.) Taub for lac production in India  

Microsoft Academic Search

Lac, a natural resin produced by the lac insect Kerria lacca, is an important income-generating non-timber forest product in India. It is expected that the potential for lac production could increase with India's recent changes in forest policy which encourage the natural regeneration of degraded lands. A review is presented on improved management systems of lac production on Butea monosperma

Vanessa Sequeira; Peter G. Bezkorowajnyj

1998-01-01

278

Antihyperglycemic and antioxidative attribute of hydroethanolic extract of Butea monosperma (Lam.) seeds and its active constituents.  

PubMed

Treatment of diabetic mice with glibenclamide and crude extract (BE) significantly declined the FBG content. However, amongst the 6 isolated compounds, 3 compounds (C1, C4 and C6) appreciably subsided the exaggerated level of FBG. Simultaneously, glibenclamide, BE, C4 and C6 treatment markedly enhanced the hepatic glycogen content as compared to diabetic control group. Administration of crude extract, C4, C5 and C6 also exerted a protective effect on the declined activity of SOD, CAT and GSH-Px in the three tissues. However, all the herbal treatments produced a pronounced escalation in GSH content. Contrarily the elevated level of hepatic, pancreatic and renal TBARS monitored in diabetic animals was significantly diminished in treated groups of animals. Alloxan administration severely deteriorated the structure of liver and pancreas of diabetic mice, which was found to be restored to a certain extent in glibenclamide, BE and C6 treated animals. Identification of the most potent antihyperglycemic compound C6 by HPLC confirmed its triterpene nature. C6 was then further characterized via various spectroscopic methods (IR, NMR and Mass) that revealed its similarity with laccijalaric ester-I, a triterpene present in soft resin of B. monosperma seeds. PMID:22013742

Sharma, Nidhi; Garg, Veena

2011-10-01

279

Genome Sequence of the Sulfate-Reducing Bacterium Desulfotomaculum hydrothermale Lam5T  

E-print Network

and thermal ecosystems (1). Evi- dence of their presence in terrestrial geothermal hot springs has been a Tunisian hot spring. The genome is composed of 2.7 Mb, with a G C content of 49.48%, and it contains 2 draft genome sequence of a Desulfotomaculum spe- cies isolated from a terrestrial hot spring in Tunisia

Boyer, Edmond

280

Virtual Private Networks Based Trusted Third Parties Services Alex Galis, John Lam, Walter Eaves  

E-print Network

the on-going development effort in the IST project called HARP (Harmonisation for the security of web. The TTP solutions are developed in the HARP project [1] by designing VPN security services, tools, by means of the HTTP protocol. 2. The HARP Project and Motivation TTP services can be considered as value

Haddadi, Hamed

281

Caihuan Ke K. N. Yu P. K. S. Lam Wen-Xiong Wang  

E-print Network

stations, fuel reprocessing plants and waste dis- posal installations, has resulted in the release of many or- ganisms, including potential transfer to the top trophic levels. Among the many radionuclides may be present in the low level aqueous radioactive wastes discharged from nuclear fa- cilities

Yu, K.N.

282

Recognizing Faces Showing Expressions Yaser Yacoob, HeungMan Lam and Larry S. Davis  

E-print Network

@cs.umd.edu, lsd@umiacs.umd.edu Computer Vision Laboratory Center for Automation Research University of Maryland to be robust to local changes (a fact that can play against it if the similarity between the faces is high

Yacoob, Yaser

283

Establishment of bermudagrass (Cynodon dactylon (L.) Pers.) seeded with annual ryegrass (Lolium multiflorum Lam.  

E-print Network

plants were in the boot stage, The herbicide applica- tions were made using a knap-sack sprayer equipped with a 3- nozzle boom to cover a 5-foot swath. A constant nozzle pressure of 35 psi was maintained with a pressure regulator. Volumes of ap... plants were in the boot stage, The herbicide applica- tions were made using a knap-sack sprayer equipped with a 3- nozzle boom to cover a 5-foot swath. A constant nozzle pressure of 35 psi was maintained with a pressure regulator. Volumes of ap...

Bowmer, William Jackson

2012-06-07

284

Evaluation of cytotoxicity of Moringa oleifera Lam. callus and leaf extracts on Hela cells  

PubMed Central

Background: There are considerable attempts worldwide on herbal and traditional compounds to validate their use as anti-cancer drugs. Plants from Moringaceae family including Moringa oleifera possess several activities such as antitumor effect on tumor cell lines. In this study we sought to determine if callus and leaf extracts of M. oleifera possess any cytotoxicity. Materials and Methods: Ethanol-water (70-30) extracts of callus and leaf of M. oleifera were prepared by maceration method. The amount of phenolic compounds of the extracts was determined by Folin Ciocalteu method. The cytotoxicity of the extracts against Hela tumor cells was carried out using MTT assay. Briefly, cells were seeded in microplates and different concentrations of the extract were added. Cells were incubated for 48 h and their viability was evaluated by addition of tetrazolium salt solution. After 3 h medium was aspirated, dimethyl sulfoxide was added and absorbance was determined at 540 nm with an ELISA plate reader. Cytotoxicity was considered when more than 50% reduction on cell survival was observed. Results: Callus and leaf extracts of M. oleifera significantly decreased the viability of Hela cells in a concentration-dependent manner. However, leaf extract of M. oleifera were more potent than that of callus extract. Conclusion: As the content of phenolic compounds of leaf extract was higher than that of callus extract, it can be concluded that phenolic compounds are involved in the cytotoxicity of M. oleifera. PMID:25337524

Jafarain, Abbas; Asghari, Gholamreza; Ghassami, Erfaneh

2014-01-01

285

Machinability improvement of titanium alloy (Ti–6Al–4V) via LAM and hybrid machining  

Microsoft Academic Search

Titanium alloy (Ti–6Al–4V) is one of the materials extensively used in the aerospace industry due to its excellent properties of high specific strength and corrosion resistance, but it also presents problems wherein it is an extremely difficult material to machine. The cost associated with titanium machining is also high due to lower cutting speeds (<60m\\/min) and shorter tool life. Laser-assisted

Chinmaya R. Dandekar; Yung C. Shin; John Barnes

2010-01-01

286

Seeding rate and seed size as management techniques for ryegrass (Lolium Multiflorum, Lam) in winter wheat  

E-print Network

Higher seeding rates and larger seed sizes could enhance the competitiveness of wheat with ryegrass. Growth room and field research evaluated the effects of wheat seeding rates and seed size in competition with Italian ryegrass. Winter wheat seeds...

Cook, Casey Lee

2005-08-29

287

Integrating LMSs in the Educational Process: Greek Teachers' Initial Perceptions about LAMS  

ERIC Educational Resources Information Center

E-learning with the use of Learning Management Systems, has been increasingly adopted in Primary, Secondary and Higher Education with the expectation to increase students' motivation and infuse activity-centred learning strategies with various educational benefits. This study has investigated the initial perceptions of Greek teachers about the…

Papadakis, Spyros; Dovros, Nikos; Paschalis, Giorgos; Rossiou, Eleni

2012-01-01

288

Identification, distribution, and control of triasulfuron-resistant Italian ryegrass (Lolium multiflorum Lam.) in Central Texas  

E-print Network

is attributed to increased herbicide metabolism. The mechanism of ALS inhibitor resistance in kochia (Kochia scoparia L. ), prickly lettuce, common waterhemp (Amaranrhris rudis Sauer. ), common chickweed (Stellaria media L. Vill. ), and palmer amaranth... is attributed to increased herbicide metabolism. The mechanism of ALS inhibitor resistance in kochia (Kochia scoparia L. ), prickly lettuce, common waterhemp (Amaranrhris rudis Sauer. ), common chickweed (Stellaria media L. Vill. ), and palmer amaranth...

Tucker, Kevin Patrick

2012-06-07

289

Eliminating Bottlenecks in Overlay Multicast Min Sik Kim, Yi Li, and Simon S. Lam  

E-print Network

systems provide more flexibility in topology con- struction, but consume more bandwidth of an underlying the right number of children is non-trivial; fan-out should be a function of the available bandwidth to each, another edge must be added to maintain connectivity. But the newly added edge may cause another bottleneck

Lam, Simon S.

290

Eliminating Bottlenecks in Overlay Multicast Min Sik Kim Yi Li Simon S. Lam  

E-print Network

multicast systems provide more flexibility in topol- ogy construction, but consume more bandwidth the right number of children is non-trivial; fan-out should be a function of the available bandwidth to each be added to maintain con- nectivity. But the newly added edge may cause an- other bottleneck. Even worse

Kim, Min Sik

291

Eliminating Bottlenecks in Overlay Multicast Min Sik Kim, Yi Li, and Simon S. Lam  

E-print Network

systems provide more flexibility in topology con- struction, but consume more bandwidth of the underlying the right number of children is non-trivial; fan-out should be a function of the available bandwidth to each be done very carefully. When a tree edge is cut, another edge must be added to maintain connectivity

Kim, Min Sik

292

NASA FOIA PUBLIC LIAISON OFFICERS and REQUESTER SERVICE CENTERS Miriam Brown-Lam  

E-print Network

20546 (202) 358-2575 foiaoig@hq.nasa.gov Jet Propulsion Laboratory (JPL) Dennis Mahon JPL FOIA Public (818) 393-6779 jpl-foia@nasa.gov Johnson Space Center (JSC) Jessica Cordero JSC FOIA Public Liaison

Waliser, Duane E.

293

Leucanthemum vulgare lam. germination, growth and mycorrhizal symbiosis under crude oil contamination.  

PubMed

Oil contamination of soil limits plants' access to water and nutrients. Leucanthemum vulgare colonized by mycorrhizae could provide an effective tool in remedying oil contamination. Seeds of L. vulgare were planted in pots containing soil mixed with petroleum at 0, 2.5, 5, 7.5, and 10% w/w and propagules of mycorrhizal fungi. Plants were grown under ambient conditions for 16 weeks. Seed germination data were collected weekly for three weeks. Mycorrhizal percentage, spore counts, length and weight of roots and shoots were determined after harvesting. Results showed significant differences in seed germination rates between oil-treated, mycorrhizal and non-mycorrhizal plants. The overall germination rate was greater at 7.5% w/w crude oil contamination (p = 0.05) in mycorrhizal and non-mycorrhizal pots with significant differences between their respective Root:Shoot ratios (both length and weight). Results of this research showed L. vulgare could be germinated and grown in crude oil contaminated soils and could be used to augment plant establishment as part of phytoremediation practices. PMID:24933896

Noori, Azam Sadat; Maivan, Hassan Zare; Alaie, Ebrahim

2014-01-01

294

Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells  

PubMed Central

Background Bryophyllum pinnata (B. pinnata) is a common medicinal plant used in traditional medicine of India and of other countries for curing various infections, bowel diseases, healing wounds and other ailments. However, its anticancer properties are poorly defined. In view of broad spectrum therapeutic potential of B. pinnata we designed a study to examine anti-cancer and anti-Human Papillomavirus (HPV) activities in its leaf extracts and tried to isolate its active principle. Methods A chloroform extract derived from a bulk of botanically well-characterized pulverized B. pinnata leaves was separated using column chromatography with step- gradient of petroleum ether and ethyl acetate. Fractions were characterized for phyto-chemical compounds by TLC, HPTLC and NMR and Biological activity of the fractions were examined by MTT-based cell viability assay, Electrophoretic Mobility Shift Assay, Northern blotting and assay of apoptosis related proteins by immunoblotting in human cervical cancer cells. Results Results showed presence of growth inhibitory activity in the crude leaf extracts with IC50 at 552 ?g/ml which resolved to fraction F4 (Petroleum Ether: Ethyl Acetate:: 50:50) and showed IC50 at 91 ?g/ml. Investigations of anti-viral activity of the extract and its fraction revealed a specific anti-HPV activity on cervical cancer cells as evidenced by downregulation of constitutively active AP1 specific DNA binding activity and suppression of oncogenic c-Fos and c-Jun expression which was accompanied by inhibition of HPV18 transcription. In addition to inhibiting growth, fraction F4 strongly induced apoptosis as evidenced by an increased expression of the pro-apoptotic protein Bax, suppression of the anti-apoptotic molecules Bcl-2, and activation of caspase-3 and cleavage of PARP-1. Phytochemical analysis of fraction F4 by HPTLC and NMR indicated presence of activity that resembled Bryophyllin A. Conclusions Our study therefore demonstrates presence of anticancer and anti-HPV an activity in B. pinnata leaves that can be further exploited as a potential anticancer, anti-HPV therapeutic for treatment of HPV infection and cervical cancer. PMID:22405256

2012-01-01

295

Antibacterial activity of bark extracts of Moringa oleifera Lam. against some selected bacteria.  

PubMed

The methanol, chloroform, ethyl acetate and aqueous bark extracts of Moringa oleifera were evaluated for their antibacterial activity against four bacteria viz. Staphylococcus aureus, Citrobacter freundii, Bacillus megaterium and Pseudomonas fluorescens using erythromycin as positive control. The activity was analyzed using paper disc diffusion method at different concentration of the extract. The study revealed that all the bark extracts irrespective of their types, in different concentrations inhibited growth of the test pathogens to varying degrees. Ethyl acetate extract showed maximum activity against all the bacterial strains followed in descending order by chloroform, methanol and aqueous extracts. The activity decreased with decrease in concentration of the extract. Staphylococcus aureus was found to be the most sensitive test organism to different extracts of Moringa oleifera. Looking to these results it may be concluded that M. oleifera may be a potential source for the treatment of different infections caused by the resistant microbes. PMID:25362592

Zaffer, Mudasser; Ahmad, Showkat; Sharma, Rajendra; Mahajan, Surabhi; Gupta, Ankur; Agnihotri, Rajneesh Kumar

2014-11-01

296

Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam  

Microsoft Academic Search

Moringa oleifera is an important multipurpose tree introduced to Africa from India at the turn of this century. Despite limited knowledge of the levels of genetic diversity and relatedness of introduced populations, their utilization as a source of seed for planting is widespread. In order to facilitate reasoned scientific decisions on its management and conservation and prepare for a selective

G. M. MULUVI; J. I. SPRENT; N. SORANZO; J. PROVAN; D. ODEE; G. FOLKARD; J. W. McNICOL; W. POWELL

1999-01-01

297

Removal of Zn(II) ions from aqueous solution using Moringa oleifera Lam. (horseradish tree) biomass  

Microsoft Academic Search

The removal of Zn(II) ions from aqueous solution using pure and chemically pretreated biomass of Moringa oleifera was investigated at 30±1°C in this study. The experimental results explored that the maximum pH (pHmax) for efficient sorption of Zn(II) was 7±0.1 at which evaluated biosorbent dosage and biosorbent particle size, were 0.5g\\/L, <0.255mm, respectively. The cellular Zn(II) concentration increased with the

Haq Nawaz Bhatti; Beenish Mumtaz; Muhammad Asif Hanif; Raziya Nadeem

2007-01-01

298

Antimicrobial and Antioxidant Activities of Bauhinia racemosa Lam. and Chemical Content  

PubMed Central

Methanol 70% extract of Bauhinia racemosa aerial parts was tested for antimicrobial activity against different bacterial and fungal strains and for antioxidant activity and also total content of polyphenols with phytochemical analysis of the extract was determined. The results have showed that the extract has a significant antimicrobial activity, it inhibited the growth of Bacillus subtilis and also it was highly active against Candida albicans suggesting that it can be used in the treatment of fungal infections. The extract has a good antioxidant activity, it has shown high values of the trolox equivalent antioxidant capacity and polyphenol content while it has shown a lower value of oxygen radical absorbance capacity. Phytochemical analysis has shown that it has interesting phytochemical bioconstituents, include flavonoids, tannins and others, and the deep phytochemical study results in the isolation of methyl gallate, gallic, kaempferol, quercetin, querection 3–O–?–rhamnoside, kaempferol 3–O–?–glucoside, myricetin–3–O–?– glucoside, querection–3–O–rutinoside (Rutin). All compounds were identified by different spectroscopic analyses (UV, 1H–NMR, 13C–NMR) and Mass Spectrometry (MS).

Rashed, Khaled; Butnariu, Monica

2014-01-01

299

Biological Invasions 1: 189201, 1999. 1999 Kluwer Academic Publishers. Printed in the Netherlands.  

E-print Network

weeds including the common reed (Phragmites australis (Cav.) Trin.), purple loosestrife (Lythrum salicaria L.), and kudzu (Pueraria lobata (Willd.) Ohwi). Others, including Japanese barberry (Berberis

Silander Jr., John A.

300

Data Dependence and DataFlow Analysis of Arrays Dror E. Maydan, Saman P. Amarasinghe and Monica S. Lam  

E-print Network

Data Dependence and Data­Flow Analysis of Arrays Dror E. Maydan, Saman P. Amarasinghe and Monica S analysis is crucial in the development of compiler technology. For the abstraction to be useful, it must be tractable to extract the information from at least a large enough set of programs. Data dependence, distance

Amarasinghe, Saman

301

Hung Van Lam, hELLA HUNG and the Hung Dynasty: Agency and Social Intelligibility in Performance on the Margins  

E-print Network

limits. As with many reality TV shows, the more outrageous,The power of reality TV's draw towards the "freak show," theREALITY SHOW CONTESTANT Up until his appearance as hELLA HUNG on the seventh season of the Fox TV show

Woon, Christopher David

2012-01-01

302

The Collective: A Cache-Based System Management Architecture Ramesh Chandra Nickolai Zeldovich Constantine Sapuntzakis Monica S. Lam  

E-print Network

that converts a PC into a client; finally, they can use a remote display client from a browser to access their desktop running on a remote server. We have developed a prototype of the Collective sys- tem and have used on a LAN, WAN with broadband, or even computers occasionally disconnected from the network like a laptop

Levis, Philip

303

Constituents of Cajanus cajan (L.) Millsp., Moringa oleifera Lam., Heliotropium indicum L. and Bidens pilosa L. from Nigeria.  

PubMed

The essential oils of four plant species from Nigeria have been extracted by hydrodistillation and analyzed by GC and GC-MS. The oils of Cajanus cajan were comprised of sesquiterpenes (92.5%, 81.2% and 94.3% respectively in the leaves, stem and seeds). The major compounds identified were alpha-himachalene (9.0-11.5%), beta-himachalene (8.0-11.0%), gamma-himachalene (6.9-8.1%), alpha-humulene (7.1-8.7%) and alpha-copaene (4.5-5.6%). However, monoterpenoid compounds (81.8%) dominated the oil of Moringa oleifera with an abundance of alpha-phellandrene (25.2%) and p-cymene (24.9%). On the other hand, aldehydes (52.8%) occurred in the highest amount in Heliotropium indicum, represented by phenylacetaldehyde (22.2%), (E)-2-nonenal (8.3%) and (E, Z)-2-nonadienal (6.1%), with a significant quantity of hexahydrofarnesylacetone (8.4%). The leaf and stem oils of Bidens pilosa were dominated by sesquiterpenes (82.3% and 59.3%, respectively). The main compounds in the leaf oil were caryophyllene oxide (37.0%), beta-caryophyllene (10.5%) and humulene oxide (6.0%), while the stem oils had an abundance of hexahydrofarnesyl acetone (13.4%), delta-cadinene (12.0%) and caryophyllene oxide (11.0%). The observed chemical patterns differ considerably from previous investigations. PMID:19476009

Ogunbinu, Akinola O; Flamini, Guido; Cioni, Pier L; Adebayo, Muritala A; Ogunwande, Isiaka A

2009-04-01

304

Bioactivity of Piper guineense Schum. & Thonn seed and Moringa oleifera Lam. leaf powder against Trogoderma granarium Everts (Coleoptera: Dermestidae)  

Microsoft Academic Search

Laboratory studies were carried out to investigate bioactivity of Piper guineense seeds and Moringa oleifera leaf powders applied singly or in a mixture against larvae and adult Trogoderma granarium Everts in airtight containers. Three levels (0.0 g, 0.5 g, and 1.0 g\\/20 g groundnut seeds) of the plant powders were used and pirimiphos-methyl was applied at 0.01 g\\/20 g seeds (recommended dose). Another control consisting of

Samuel Adelani Babarinde; Olufemi O. Richard Pitan; Adekunle Tosin Ogunfiade

2011-01-01

305

The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves  

Microsoft Academic Search

Moringa oleifera is used in Thai traditional medicine as cardiotonic. Recent studies demonstrated its hypocholesterolaemic effect. However, to be clinically useful, more scientific data are needed. Aim of the Study: We investigated the antioxidant, hypolipidaemic and antiatherosclerotic activities of Moringa oleifera leaf extract. Materials and Methods: Scavenging activity of the extract on 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), and the inhibitory effect on

Pilaipark Chumark; Panya Khunawat; Yupin Sanvarinda; Srichan Phornchirasilp; Noppawan Phumala Morales; Laddawal Phivthong-ngam; Piyanee Ratanachamnong; Supath Srisawat; Klai-upsorn S. Pongrapeeporn

2008-01-01

306

Anti-ulcer activity of the alkali preparation of the root and fresh leaf juice of moringa oleifera lam.  

PubMed

The alkali preparation of the root and fresh leaf juice of Moringa oleifera possessed significant dose -depen-dent anti-ulcer activity in experimentally induced acute gastric ulcers with aspirin, the anti-ulcer effect of the alkali preparation of the root seems to be more pronounced than that of the fresh leaf juice. Te anti-ulcer activity of the alkali preparation of the root could be due to its content of alkaloids or its anticholinergic and antihistaminic activities, or a combination of these factors. PMID:22556845

Ruckmani, K; Kavimani, S; Jayakar, B; Anandan, R

1998-01-01

307

Genetic Dissection of New Genotypes of Drumstick Tree (Moringa oleifera Lam.) Using Random Amplified Polymorphic DNA Marker  

PubMed Central

The knowledge of genetic diversity of tree crop is very important for breeding and improvement program for the purpose of improving the yield and quality of its produce. Genetic diversity study and analysis of genetic relationship among 20 Moringa oleifera were carried out with the aid of twelve primers from, random amplified polymorphic DNA marker. The seeds of twenty M. oleifera genotypes from various origins were collected and germinated and raised in nursery before transplanting to the field at University Agricultural Park (TPU). Genetic diversity parameter, such as Shannon's information index and expected heterozygosity, revealed the presence of high genetic divergence with value of 1.80 and 0.13 for Malaysian population and 0.30 and 0.19 for the international population, respectively. Mean of Nei's gene diversity index for the two populations was estimated to be 0.20. In addition, a dendrogram constructed, using UPGMA cluster analysis based on Nei's genetic distance, grouped the twenty M. oleifera into five distinct clusters. The study revealed a great extent of variation which is essential for successful breeding and improvement program. From this study, M. oleifera genotypes of wide genetic origin, such as T-01, T-06, M-01, and M-02, are recommended to be used as parent in future breeding program. PMID:23862149

Rufai, Shamsuddeen; Hanafi, M. M.; Rafii, M. Y.; Ahmad, S.; Arolu, I. W.; Ferdous, Jannatul

2013-01-01

308

Nutrient and Antinutrient Contents in Raw and Cooked Young Leaves and Immature Pods Of Moringa oleifera, Lam  

Microsoft Academic Search

The nutrient composition and anti-nutrient contents of raw and cooked Moringa leaves and immature pods were analyzed. Cooking caused significant reductions (p ? 0.05) in the contents of crude protein, crude fiber, ether extracts, ash, ascorbic acid, and ?-carotene in the leaves and pods. Significant reductions (p ? 0.05) also were observed in Ca, Mg, Zn, P, Na, Cu, and

A. B. Gidamis; J. T. Panga; S. V. Sarwatt; B. E. Chove; N. B. Shayo

2003-01-01

309

UBC Social Ecological Economic Development Studies (SEEDS) Student Report Haoyi Liu, Hinson Tsang, Michael Lam, Terranda Yeung  

E-print Network

, individual thermal comfort differs in age, metabolic rate, gender, and many other factors. Personal heaters are commonly used in offices and workplaces to address the problem. A typical convection heater needs 1500 W to operate, which is equal to fifteen 100 W light bulbs operating at the same time in a single room

310

Antimicrobial and antioxidant activities of the extracts and compounds from the leaves of Psorospermum aurantiacum Engl. and Hypericum lanceolatum Lam.  

PubMed Central

Background Psorospermun aurantiacum and Hypericum lanceolatum are plants locally used in Cameroon and other parts of Africa for the treatment of gastrointestinal and urinary tract infections, skin infections, venereal diseases, gastrointestinal disorder, infertility, epilepsy as well as microbial infections. The present study was designed in order to investigate the in vitro antimicrobial and radical scavenging activities of the extracts and isolated compounds from the leaves of these plants. Methods The plant extract was prepared by maceration in ethyl acetate and methanol and fractionated by column chromatography. The structures of isolated compounds were elucidated by spectroscopic analyses in conjunction with literature data. The broth microdilution method was used to evaluate the in vitro antimicrobial activity against bacteria, yeasts and dermatophytes. The antioxidant potentials of the extracts and their isolated compounds were evaluated using the DPPH radical scavenging method. Results Five known compounds: physcion (1), 1,8-dihydroxy-3-geranyloxy-6-methylanthraquinone (2), kenganthranol B (3), vismiaquinone (4), and octacosanol (5) were isolated from the leaves of P. aurantiacum while six compounds including friedelin (6), betulinic acid (7), 2,2’,5,6’-tetrahydroxybenzophenone (8), allanxanthone A (9), 1,3,6- trihydroxyxanthone (10) and isogarcinol (11) were isolated from H. lanceolatum. Compound 8 and 4 exhibited the highest antibacterial and antifungal activities with MIC ranges of 2–8??g/ml and 4–32??g/ml respectively. P. aurantiacum crude extract (Rsa50?=?6.359?±?0.101) showed greater radical scavenging activity compared with H. lanceolatum extract (Rsa50?=?30.996?±?0.879). Compound 11 showed the highest radical scavenging activity (RSa50?=?1.012?±?0.247) among the isolated compounds, comparable to that of L-arscobic acid (RSa50?=?0.0809?±?0.045). Conclusions The experimental findings show that the ethyl acetate and methanol extracts and isolated compounds from P. aurantiacum and H. lanceolatum stem bark possess significant antimicrobial and antioxidant activities justifying the use of these plants in traditional medicine, which may be developed as phytomedicines. PMID:22916964

2012-01-01

311

Contact Angle Hysteresis, Adhesion, and Marine Biofouling Donald L. Schmidt, Robert F. Brady, Jr.,*, Karen Lam, Dale C. Schmidt, and  

E-print Network

Contact Angle Hysteresis, Adhesion, and Marine Biofouling Donald L. Schmidt, Robert F. Brady, Jr, 2004 Adhesive and marine biofouling release properties of coatings containing surface, adhesion, and marine biofouling were studied. Adhesion was determined by peel tests using pressure

Chaudhury, Manoj K.

312

Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam.  

PubMed

Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines. PMID:25189525

Rathi Sre, P R; Reka, M; Poovazhagi, R; Arul Kumar, M; Murugesan, K

2015-01-25

313

An Algorithm for Lossless Smoothing of MPEG Video* Simon S. Lam, Simon Chow, and David K, Y. Yau  

E-print Network

of encoder output from one picture to the next; without smoothing, the performance of networks that carry from Lockheed. Permission to copy without fee all or part of this material is granted provided that the copies are not made or diskibuted for direct commercial advantage, the ACM copyright notice and the title

Lam, Simon S.

314

Why Steiner-tree type algorithms work for community detection Mung Chiang Henry Lam Zhenming Liu Vincent Poor  

E-print Network

Con- ference on Artificial Intelligence and Statistics (AISTATS) 2013, Scottsdale, AZ, USA. Volume 31 example is the placement of personal- ized ads in social network services such as Facebook or Linkedin class, it essentially needs to find a commu- nity in the town that is interested in foreign languages

Lam, Henry

315

Aplicação de cromatografia centrífuga de contra-corrente na purificação de ácido ursólico das folhas de Eugenia brasiliensis Lam  

Microsoft Academic Search

13 C-RMN, GC-EM e co-injeção com padrão comercial em CG-DIC, na forma do éster metílico. A técnica de CCCAV, usualmente usada para triterpenos glicosilados, foi aqui aplicada para a aglicona. As fases móvel e estacionária, no experimento de CCCAV, foram geradas pela mistura de n-hexano : acetato de etila : metanol : água, na proporção 10:5:2,5:1. A seleção do sistema

N. Frighetto; R. M. Welendorf; A. M. P. Silva; M. J. Nakamura; A. C. Siani

2005-01-01

316

Effects of olive mill wastewater physico-chemical treatments on polyphenol abatement and Italian ryegrass (Lolium multiflorum Lam.) germinability.  

PubMed

Direct spreading on agricultural lands may represent an environmentally friendly disposal method and a possible use of water and nutrients from olive mill wastewaters (OMWs). However, the agronomic use of OMWs is limited, among others by polyphenols, which exert phytotoxic effects. Activated charcoal (AC) has been recognized as a very effective agent for polyphenol abatement, as it enables an irreversible process of phenol adsorption. Addition of calcium hydroxide (Ca(OH)2) has also been described as a cheap and effective method in polyphenols abatement. However, the effects of Ca(OH)2 addition to OMW on seed germination are unclear. In this paper, the effects of AC and/or Ca(OH)2 on OMW polyphenols abatement, and Lolium multiflorum seed germination have been investigated. The highest polyphenols removal, approximately 95%, was observed when 80 g L(-1) of AC was added to OMWs (the maximum dose in this investigation). The addition of Ca(OH)2 not only improved the effectiveness of the AC treatment but also resulted in a significant rise in Lolium seed germination at the highest AC doses (60 and 80 g L(-1)). Considering the high salinity (7300 ?S cm(-1)) of these wastewaters, low quantities of Ca(OH)2 may also exert a protective effect on soil structure counteracting the sodium-induced dispersion through the binding action of calcium cation on clays and organic matter. PMID:24289894

Barbera, A C; Maucieri, C; Ioppolo, A; Milani, M; Cavallaro, V

2014-04-01

317

Asymmetric somatic hybridization between tall fescue ( Festuca arundinacea Schreb.) and irradiated Italian ryegrass ( Lolium multiflorum Lam.) protoplasts  

Microsoft Academic Search

Intergeneric asymmetric somatic hybrids have been obtained by the fusion of metabolically inactivated protoplasts from embryogenic suspension cultures ofFestuca arundinacea (recipient) and protoplasts from a non-morphogenic cell suspension ofLolium multiflorum (donor) irradiated with 10, 25, 50, 100, 250 and 500 Gy of X-rays. Regenerating calli led to the recovery of genotypically and phenotypically different asymmetric somatic hybridFestulolium plants. The genome

G. Spangenberg; M. P. Vallés; Z. Y. Wang; P. Montavon; J. Nagel; I. Potrykus

1994-01-01

318

Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass ( Lolium multiflorum Lam.)  

Microsoft Academic Search

Bacterial wilt caused by Xanthomonas translucens pv. graminis (Xtg) is a major disease of economically important forage crops such as ryegrasses and fescues. Targeted breeding based on seedling inoculation has resulted in cultivars with considerable levels of resistance. However, the mechanisms of inheritance of resistance are poorly understood and further breeding progress is difficult to obtain. This study aimed to

Bruno Studer; Beat Boller; Doris Herrmann; Eva Bauer; Ulrich K. Posselt; Franco Widmer; Roland Kölliker

2006-01-01

319

Consistent detection of QTLs for crown rust resistance in Italian ryegrass ( Lolium multiflorum Lam.) across environments and phenotyping methods  

Microsoft Academic Search

Crown rust, caused by Puccinia coronata f. sp. lolii, is one of the most important diseases of temperate forage grasses, such as ryegrasses (Lolium spp.), affecting yield and nutritional quality. Therefore, resistance to crown rust is a major goal in ryegrass breeding\\u000a programmes. In a two-way pseudo-testcross population consisting of 306 Lolium multiflorum individuals, multisite field evaluations as well as

Bruno Studer; Beat Boller; Eva Bauer; Ulrich K. Posselt; Franco Widmer; Roland Kölliker

2007-01-01

320

Evaluating the last remnants of Butea monosperma (Lam.) Kuntze Forest for their in situ conservation: a case study.  

PubMed

This paper provides a suitable framework for consolidating knowledge and providing a useful starting point to undertake conservation of the last remnants of Butea monosperma forest. A survey was conducted to assess the status of a reportedly unexplored B. monosperma forest in a narrow strip of land along the northeastern region of Pakistan in order to conserve the remaining remnants. A preliminary reconnaissance survey on the basis of land use divides the narrow stretch of land, with concentration of remnants into four zones. The remnants were further evaluated for in situ conservation by a scoring system based on vegetation analysis and quality of habitat. Low value was assigned to those criteria which had negative impact and high to those which had positive impact on performance of B. monosperma and its long-term survival. Additional information, pertaining to decline, trends of change in the land use, and the underlying socioeconomic processes, was also made. Information from the study area indicated that, in the past two decades, the decline and conversion of the remaining B. monosperma forest to agriculture and forestry has been intensified. The vegetation analysis revealed that all remnants are broadly B. monosperma-Adhatoda vasica, bispecies association. The scoring system ranked the remnants into four classes. Two forests from the intact remnant class and one from the mildly degraded class were recommended for in situ conservation. Keeping in view the deteriorating status of the highly ranked remnants across the landscape and the degree of alteration it is undergoing, they were assigned the conservation status category of "critical." An approach suggested for their immediate conservation was to fence them, with open access to the local people for sustainable resource use. In situ conservation of these benchmarks would help in further understanding the ecological, evolutionary, and structural features of this unique biogeographic ecotone and its affinities with the adjacent ecosystems. PMID:19894131

Khan, Amin U

2010-11-01

321

Inhibition of testicular and Vipera russelli snake venom hyaluronidase activity by Butea monosperma (Lam) Kuntze stem bark  

Microsoft Academic Search

This study was carried out to investigate the anti-fertility and anti-venom activities of the extract of the stem bark of Butea monosperma by inhibiting hyaluronidase, which is a spreading factor and plays a role in fertilisation. Among ethanol, methanol and water extracts, the ethanol extract dose-dependently inhibited the ovine, mouse testicular and Vipera russelli snake venom hyaluronidase enzyme activities, with

Shaista Tarannum; Riyaz Mohamed; Bannikuppe S. Vishwanath

2011-01-01

322

Evaluating the last remnants of Butea monosperma (Lam.) Kuntze Forest for their in situ conservation: a case study  

Microsoft Academic Search

This paper provides a suitable framework for consolidating knowledge and providing a useful starting point to undertake conservation\\u000a of the last remnants of Butea monosperma forest. A survey was conducted to assess the status of a reportedly unexplored B. monosperma forest in a narrow strip of land along the northeastern region of Pakistan in order to conserve the remaining remnants.

Amin U. Khan

2010-01-01

323

Inhibition of testicular and Vipera russelli snake venom hyaluronidase activity by Butea monosperma (Lam) Kuntze stem bark  

Microsoft Academic Search

This study was carried out to investigate the anti-fertility and anti-venom activities of the extract of the stem bark of Butea monosperma by inhibiting hyaluronidase, which is a spreading factor and plays a role in fertilisation. Among ethanol, methanol and water extracts, the ethanol extract dose-dependently inhibited the ovine, mouse testicular and Vipera russelli snake venom hyaluronidase enzyme activities, with

Shaista Tarannum; Riyaz Mohamed; Bannikuppe S. Vishwanath

2012-01-01

324

Antihyperglycemic and antioxidative potential of hydroalcoholic extract of Butea monosperma Lam flowers in alloxan-induced diabetic mice  

Microsoft Academic Search

for 45 days significantly lowered blood glucose level thereby preventing steep onset of hyperglycemia which was observed after alloxan administration and maintained body weight and blood glucose level close to the values observed in normal control and glibenclamide-treated diabetic mice. Moreover, the level of serum total cholesterol, triglyceride, low-density lipoprotein and very low-density lipoprotein cholesterol were also lowered, whereas the

Nidhi Sharma; Veena Garg

2009-01-01

325

Free radical scavenging activity, phenolic contents and phytochemical evaluation of different extracts of stem bark of Butea monosperma (Lam.) Kuntze  

Microsoft Academic Search

The antioxidant activity, phenolic contents and phytochemical profile of acetone, methanol, water and ethyl acetate extracts of the stem bark of B. monosperma were investigated. Extracts showed highest antioxidant activity at 100 ?g ml with application of different assays: 2?-2? diphenyl–2? picrylhydrazyl (DPPH) scavenging (83.5% and 78.1%), reducing power (1.85 and 1.78), deoxyribose degradation site-specific (65.5% and 61.5%) and non-site-specific (67.1% and

Raj Kumar Salar; Leena Seasotiya

2012-01-01

326

Antihyperglycemic and antioxidative potential of hydroalcoholic extract of Butea monosperma Lam flowers in alloxan-induced diabetic mice.  

PubMed

Daily treatment of alloxan-induced diabetic animals with 50% ethanolic extract of B. monosperma flowers (BMEE) for 45 days significantly lowered blood glucose level thereby preventing steep onset of hyperglycemia which was observed after alloxan administration and maintained body weight and blood glucose level close to the values observed in normal control and glibenclamide-treated diabetic mice. Moreover, the level of serum total cholesterol, triglyceride, low-density lipoprotein and very low-density lipoprotein cholesterol were also lowered, whereas the level of high-density lipoprotein cholesterol, which was reduced in untreated diabetic animals, was significantly elevated. Oxidative damage in the liver, pancreas and kidneys of diabetic mice as evidenced by a marked increment in the level of thiobarbituric acid reactive substances and also a distinct diminution in glutathione content was nullified by BMEE. Activities of antioxidant enzymes were also assessed in all the experimental groups. These enzymes registered a decline in their activity in diabetic animals thus revealing the damaging effects of free radicals generated due to alloxan exposure but their activities were reverted towards near normal range in BMEE-administered mice thus indicating the antioxidant efficacy of the drug in resisting oxidative damage. PMID:19761041

Sharmna, Nidhi; Garg, Veena

2009-07-01

327

Inhibition of testicular and Vipera russelli snake venom hyaluronidase activity by Butea monosperma (Lam) Kuntze stem bark.  

PubMed

This study was carried out to investigate the anti-fertility and anti-venom activities of the extract of the stem bark of Butea monosperma by inhibiting hyaluronidase, which is a spreading factor and plays a role in fertilisation. Among ethanol, methanol and water extracts, the ethanol extract dose-dependently inhibited the ovine, mouse testicular and Vipera russelli snake venom hyaluronidase enzyme activities, with IC?? values 12.00?±?0.45, 49.40?±?1.58?µg and 125.42?±?2.82?µg?mL?¹, respectively. In a zymogram assay, the extract showed differential inhibition towards hyaluronidase isoform preferentially with low-molecular weight isoforms. The V. russelli snake venom-induced hemorrhage was significantly reduced at 1:05 ratio of venom-to-extract in mouse. The high antioxidant activity and total phenolic content in the ethanolic extract strongly correlated with the hyaluronidase inhibition. The above results justify the traditional use of the stem bark of B. monosperma as a contraceptive and a strong antidote to snake venom. PMID:22007959

Tarannum, Shaista; Mohamed, Riyaz; Vishwanath, Bannikuppe S

2012-01-01

328

Antinocieptive and anti-inflammatory effects of Toddalia asiatica (L) Lam. (Rutaceae) root extract in Swiss albino mice  

PubMed Central

Introduction Toddalia asiatica is a commonly used medicinal plant in East Africa for the management of pain and inflammatory conditions. The present study investigated the antinociceptive and the anti-inflammatory effects of T. asiatica in Swiss albino mice. Methods The antinociceptive and the anti-inflammatory effects of T. asiatica were investigated using formalin-induced pain test and the carrageenin-induced oedema paw. The extract solvent (vehicle), aspirin and indomethacin were employed as negative and positive controls respectively. Eight mice were used in each experiment. Results In the early phase of the formalin test, the 100mg/kg dose showed no significant antinociceptive activity while the 200mg/kg showed significant (p < 0.01) antinociceptive activity. The 100 mg/kg dose showed highly significant antinociceptive activity (p < 0.001) in the late phase of the formalin test while the 200mg/kg dose showed no significant antinociceptive activity. A reduction in carragenin induced acute inflammation paw oedema was significant (p < 0.01) following administration of 100mg/kg dose but not with the 200mg/kg dose. Conclusion The present study therefore lends support to the anecdotal evidence for use of T. asiatica in the management of painful and inflammatory conditions. PMID:23734278

Kariuki, Hellen Nyambura; Kanui, Titus Ikusya; Yenesew, Abiy; Patel, Nilesh; Mbugua, Paul Mungai

2013-01-01

329

Allelopathic research of subtropical vegetation in Taiwan : III. Allelopathic exclusion of understory byLeucaena leucocephala (Lam.) de Wit.  

PubMed

Leucaena leucocephala plantations in Kaoshu, southern Taiwan, exhibit, after several years of growth, a unique pattern of weed exclusion beneathLeucaena canopy. The pattern has been observed in manyLeucaena plantations in Taiwan and is particularly pronounced in the area where a substantial amount ofLeucaena litter has accumulated on the ground. Field data showed that the phenomenon was primarily not due to physical competition involving light, soil moisture, pH, and nutrients. Instead, aqueous extracts ofLeucaena fresh leaves, litter, soil, and seed exudate showed significantly phytotoxic effects on many test species, including rice, lettuce,Acacia confusa, Alnus formosana, Casuarina glauca, Liquidambar formosana, andMimosa pudica. However, the extracts were not toxic to the growth ofLeucaena seedlings. The decomposing leaves ofLeucaena also suppressed the growth of the aforementioned plants grown in pots but did not inhibit that ofLeucaena plants. By means of paper and thin-layer chromatography, UV-visible spectrophotometry, and high-performance liquid chromatography, 10 phytotoxins were identified. They included mimosine, quercetin, and gallic, protocatechuic,p-hydroxybenzoic,p-hydroxyphenylacetic, vanillic, ferulic, caffeic, andp-coumaric acids. The mature leaves ofLeucaena possess about 5% dry weight of mimosine, the amount varying with varieties. The seed germination and radicle growth of lettuce, rice, and rye grass were significantly inhibited by aqueous mimosine solution at a concentration of 20 ppm, while that of the forest species mentioned was suppressed by the mimosine solution at 50 ppm or above. However, the growth ofMiscanthus floridulus andPinus taiwanensis was not suppressed by the mimosine solution at 200 ppm. The seedlings ofAgeratum conzoides died in mimosine solution at 50 ppm within seven days and wilted at 300 ppm within three days. It was concluded that the exclusion of understory plants was evidently due to the allelopathic effect of compounds produced byLeucaena. The allelopathic pattern was clearly shown in the area with a heavy accumulation ofLeucaena leaf litter, which was a result of drought and heavy wind influence. PMID:24307122

Chou, C H; Kuo, Y L

1986-06-01

330

Anatomy and ultrastructure alterations of Leucaena leucocephala (Lam.) inoculated with mycorrhizal fungi in response to arsenic-contaminated soil.  

PubMed

Many studies demonstrate the potential application of arbuscular mycorrhizal fungi (AMF) for remediation purposes, but little is known on AMF potential to enhance plant tolerance to arsenic (As) and the mechanisms involved in this process. We carried anatomical and ultrastructural studies to examine this symbiotic association and the characteristics of shoots and roots of Leucaena leucocephala in As-amended soils (35 and 75 mg As dm(-3)). The experiment used 3 AMF isolates from uncontaminated soils: Acaulospora morrowiae, Glomus clarum, and Gigaspora albida; a mixed inoculum derived from combining these 3 isolates (named Mix AMF); and, 3 AMF isolates from As-contaminated areas: A. morrowiae, G. clarum and Paraglomus occultum. Phytotoxicity symptoms due to arsenic contamination appeared during plant growth, especially in treatments without AMF application. Inoculation with G. clarum and the mixture of species (A. morrowiae, G. albida, and G. clarum) resulted in better growth of L. leucocephala in soils with high As concentrations, as well as significant As removal from the soil, showing a potential for using AMF in phytoextraction. Light microscopy (LS), transmission (TEM) and scanning electron microscopies (SEM) studies showed the colonization of the AMF in plant tissues and damage in all treatments, with ultrastructural changes being observed in leaves and roots of L. leucocephala, especially with the addition of 75 mg dm(-3) of As. PMID:22704769

Schneider, Jerusa; Labory, Claudia Regina Gontijo; Rangel, Wesley Melo; Alves, Eduardo; Guilherme, Luiz Roberto Guimarães

2013-11-15

331

Antiplatelet and antithrombotic activities of essential oil from wild Ocotea quixos (Lam.) Kosterm. (Lauraceae) calices from Amazonian Ecuador.  

PubMed

Ocotea quixos essential oil was shown to possess significant inhibitory activity of platelet aggregation and clot retraction in rodent plasma. This study is aimed at fully characterizing the antiplatelet activity of the whole essential oil and its main components trans-cinnamaldehyde and methyl cinnamate also in human plasma, at investigating the mechanism underlying such activity and at evaluating the potential antithrombotic activity of subacute treatment of mice with Ocotea essential oil. In vitro Ocotea essential oil and trans-cinnamaldehyde inhibited arachidonic acid-, U46619-, ADP-, phorbol12-myristate13-alcetate-, collagen-induced platelet aggregation and thrombin-induced clot retraction in human and rodent plasma; Ocotea oil and trans-cinnamaldehyde competitively antagonized contractions induced by thromboxane A2 receptor agonist U46619 in rat isolated aortic ring (K(B) = 18 and 3.2 microg ml(-1), respectively). In vivo Ocotea oil, orally administered in a subacute treatment (30-100 mg kg(-1) day(-1) for 5 days) to mice, prevented acute thrombosis induced by collagen-epinephrine intravenous injection. This antithrombotic activity was not accompanied by pro-haemorragic side effect, as detected by the inactivity in bleeding test, thus showing a favourable safety profile compared to the conventional antiplatelet agent, acetylsalicylic acid. Present findings indicate that Ocotea essential oil possesses potent and safe antithrombotic activity attributable to its antiplatelet and vasorelaxant effects. The main constituent trans-cinnamaldehyde seems to be the primary responsible for this activity through a putative mechanism involving the inhibition of thromboxane A2 receptors. PMID:17079160

Ballabeni, Vigilio; Tognolini, Massimiliano; Bertoni, Simona; Bruni, Renato; Guerrini, Alessandra; Rueda, Gabriela Moreno; Barocelli, Elisabetta

2007-01-01

332

Ocotea quixos Lam. essential oil: in vitro and in vivo investigation on its anti-inflammatory properties.  

PubMed

Here we investigated the anti-inflammatory properties of Ocotea quixos essential oil and of its main components, trans-cinnamaldehyde and methyl cinnamate, in in vitro and in vivo models. Ocotea essential oil and trans-cinnamaldehyde but not methyl cinnamate significantly reduced LPS-induced NO release from J774 macrophages at non-toxic concentrations, inhibited LPS-induced COX-2 expression and increased forskolin-induced cAMP production. The essential oil (30-100mg/kg os) and trans-cinnamaldehyde (10mg/kg os) in carrageenan-induced rat paw edema showed anti-inflammatory effect without damaging gastric mucosa. In conclusion we provide the first evidence of a significant anti-inflammatory gastro-sparing activity of O.quixos essential oil. PMID:19825398

Ballabeni, Vigilio; Tognolini, Massimiliano; Giorgio, Carmine; Bertoni, Simona; Bruni, Renato; Barocelli, Elisabetta

2010-06-01

333

UHF RFID TAG CHARACTERIZATION: OVERVIEW AND STATE-OF-THE-ART Pavel Nikitin, KVS Rao, Sander Lam  

E-print Network

each other) and free space-like environment (where the loss in the propagation channel is log, pathG is the propagation path gain. The power absorbed by the tag IC is: pGPP incabs = , (2) where p is the polarization efficiency (also called the polarization loss factor), and G are the tag impedance matching

Hochberg, Michael

334

75 FR 29722 - Foreign-Trade Zone 18-San Jose, CA; Application for Subzone; Lam Research Corporation (Wafer...  

Federal Register 2010, 2011, 2012, 2013

...steel screws, pulleys, shaft couplings, gears, machinery parts, motors, quartz fittings and parts, electrical transformers, electromagnetic couplings, batteries, induction equipment, electrothermic appliances, speakers, amplifiers,...

2010-05-27

335

Triterpenoids from Brazilian Ilex species and their in vitro antitrypanosomal activity.  

PubMed

From the leaves of Ilex affinis and Ilex buxifolia, two adulterant species of "erva mate" (Ilex paraguariensis), three new triterpenoid glycosides were isolated. Affinoside 1 (3beta-O-[beta-D-glucopyranosyl-(1-->3)-[2-O-acetyl-(1-->2

Taketa, Alexandre T C; Gnoatto, Simone C B; Gosmann, Grace; Pires, Viviane S; Schenkel, Eloir P; Guillaume, Dominique

2004-10-01

336

IR-4 Ornamental Horticulture Program Research Report Form  

E-print Network

of phytotoxicity represented is not likely to be an issue. Plant Growth. The growth measurements were quite variable due to the growth habit of this plant (Tables 2 and 6a, Figure 2a). There were no significant chlorosis and necrosis. BAS 656h EC is considered unsafe on Berberis. Plant Growth. Growth of Berberis

Lieth, J. Heinrich

337

The use of a rainfall simulator for brush control research on the Edwards Plateau region of Texas  

E-print Network

this area include ashe juniper (Juniperus ashei Buccholz), live oak (Quercus virginiana Mill.), agarita (Berberis trifolioata Moric.), Texas persimmon (Diospyros texana Scheele). Herbaceous vegetation includes Texas wintergrass (Stipa leucotricha Trim... this area include ashe juniper (Juniperus ashei Buccholz), live oak (Quercus virginiana Mill.), agarita (Berberis trifolioata Moric.), Texas persimmon (Diospyros texana Scheele). Herbaceous vegetation includes Texas wintergrass (Stipa leucotricha Trim...

Porter, Shane Courtney

2006-04-12

338

Isolation and characterization of microsatellite markers in the invasive shrub Mahonia aquifolium (Berberidaceae) and their applicability in related species  

Microsoft Academic Search

Microsatellite loci were isolated from a Mahonia aquifolium cultivar. We describe the var- iability of 10 loci in invasive European and native North American M. aquifolium and their transspecies amplification in native Mahonia repens and Mahonia pinnata from North America and one species of the related genus Berberis ( Berberis vulgaris ), native to Europe. The markers should be useful

C. ROss; W. DURKA

2006-01-01

339

Whitebrush Response to Tebuthiuron and Picloram Pellets.  

E-print Network

. Yellow thistle * - - Callicarpa americana Berberis trifoliolata Lycium berlandieri Acacia ridigula Quercus marilandica Buchloe dactyloides Leuchophyllum fru tescens Ambrosia artemisiifolia Hilaria berlangeri Lantana horrida Crataegus mollis... . Yellow thistle * - - Callicarpa americana Berberis trifoliolata Lycium berlandieri Acacia ridigula Quercus marilandica Buchloe dactyloides Leuchophyllum fru tescens Ambrosia artemisiifolia Hilaria berlangeri Lantana horrida Crataegus mollis...

Scifres, C.J.; Embry, D.L.; Mutz, J.L.

1981-01-01

340

La physique de LMD dans WRF , P. Drobinski, F. Hourdin, L. Farihead  

E-print Network

� Inclusion of GCM physics on LAM way to help on GCMs update � p. 2 #12;Introduction � GCMs start to run equations dynamics � Inclusion of GCM physics on LAM way to help on GCMs update � Challenge: Inclusion of GCM physics on LAM way to help on GCMs update � Challenge: Inclusion of LMDZ (GCM) physics in WRF (LAM

Madeleine, Jean-Baptiste

341

The Spectrum of Mutations in TSC1 and TSC2 in Women with Tuberous Sclerosis and Lymphangiomyomatosis  

Microsoft Academic Search

Lymphangiomyomatosis (LAM) is a progressive and often fatal in- terstitial lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. LAM is of unusual in- terest biologically because it affects almost exclusively young women. LAM can occur as an isolated disorder (sporadic LAM) or in association with tuberous sclerosis complex (TSC). Because only a minority

GALINA D. STRIZHEVA; THOMAS CARSILLO; WARREN D. KRUGER; EUGENE J. SULLIVAN; JAY H. RYU

342

Mutations in the tuberous sclerosis complex gene TSC2 are a cause of sporadic pulmonary lymphangioleiomyomatosis  

Microsoft Academic Search

Lymphangioleiomyomatosis (LAM) is a progressive and often fatal interstitial lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. LAM is of unusual interest biologically because it affects almost exclusively young women. LAM can occur as an isolated disorder (sporadic LAM) or in association with tuberous sclerosis complex. Renal angiomyolipomas, which are found in most

Thomas Carsillo; Aristotelis Astrinidis; Elizabeth Petri Henske

2000-01-01

343

Berberine.  

PubMed

Berberine is a plant alkaloid with a long history of medicinal use in both Ayurvedic and Chinese medicine. It is present in Hydrastis canadensis (goldenseal), Coptis chinensis (Coptis or goldenthread), Berberis aquifolium (Oregon grape), Berberis vulgaris (barberry), and Berberis aristata (tree turmeric). The berberine alkaloid can be found in the roots, rhizomes, and stem bark of the plants. Berberine extracts and decoctions have demonstrated significant antimicrobial activity against a variety of organisms including bacteria, viruses, fungi, protozoans, helminths, and chlamydia. Currently, the predominant clinical uses of berberine include bacterial diarrhea, intestinal parasite infections, and ocular trachoma infections. PMID:10767672

2000-04-01

344

Mutation analysis of the TSC1 and TSC2 genes in Japanese patients with pulmonary lymphangioleiomyomatosis  

Microsoft Academic Search

Pulmonary lymphangioleiomyomatosis (LAM) is a destructive lung disease characterized by a diffuse hamartomatous proliferation\\u000a of smooth muscle cells (LAM cells) in the lungs. Pulmonary LAM can occur as an isolated form (sporadic LAM) or in association\\u000a with tuberous sclerosis complex (TSC) (TSC-LAM), a genetic disorder with autosomal dominant inheritance with various expressivity\\u000a resulting from mutations of either the TSC1 or

Teruhiko Sato; Kuniaki Seyama; Hiroaki Fujii; Hiroshi Maruyama; Yasuhiro Setoguchi; Shin-ichiro Iwakami; Yoshinosuke Fukuchi; Okio Hino

2002-01-01

345

Chemical composition and general toxicity of essential oils extracted from the aerial parts of Artemisia armeniaca Lam. and A. incana (L.) Druce growing in Iran  

PubMed Central

The essential oils of the aerial parts of A. armeniaca and A. incana, collected from Arasbaran area (East Azarbaijan province, Iran) were extracted by hydrodistillation and analyzed by GC-MS. In total, 16 and 40 constituents were identified and quantified in the oils of A. armeniaca and A. incana representing 80.5% and 84.6% of the oils, respectively. The essential oil of A. armeniaca was mainly composed of non-terpene hydrocarbons (24.8%). The major components of the oil were ?–pinene (10.7%), nonadecane (10.0%), 6,10,14-trimethyl-2-pentadecanone (9.4%), spathulenol (7.8%) and Z-verbenol (5.8%). The essential oil of A. incana was dominated by oxygenated monoterpenes (41.6%), with camphor (20.4%), 1,8-cineol (10.3%), Z-verbenol (8.7%), ?-thujone (8.3%) and ?-thujone (5.6%), as major components. The essential oils were also subjected to general toxicity assay using brine shrimp lethality method. The toxicity profile of both oils indicated some degree of toxicity in comparison with podophyllotoxin. PMID:24459478

Mojarrab, M.; Delazar, A.; Esnaashari, S.; Afshar, F. Heshmati

2013-01-01

346

Chemical composition and general toxicity of essential oils extracted from the aerial parts of Artemisia armeniaca Lam. and A. incana (L.) Druce growing in Iran.  

PubMed

The essential oils of the aerial parts of A. armeniaca and A. incana, collected from Arasbaran area (East Azarbaijan province, Iran) were extracted by hydrodistillation and analyzed by GC-MS. In total, 16 and 40 constituents were identified and quantified in the oils of A. armeniaca and A. incana representing 80.5% and 84.6% of the oils, respectively. The essential oil of A. armeniaca was mainly composed of non-terpene hydrocarbons (24.8%). The major components of the oil were ?-pinene (10.7%), nonadecane (10.0%), 6,10,14-trimethyl-2-pentadecanone (9.4%), spathulenol (7.8%) and Z-verbenol (5.8%). The essential oil of A. incana was dominated by oxygenated monoterpenes (41.6%), with camphor (20.4%), 1,8-cineol (10.3%), Z-verbenol (8.7%), ?-thujone (8.3%) and ?-thujone (5.6%), as major components. The essential oils were also subjected to general toxicity assay using brine shrimp lethality method. The toxicity profile of both oils indicated some degree of toxicity in comparison with podophyllotoxin. PMID:24459478

Mojarrab, M; Delazar, A; Esnaashari, S; Afshar, F Heshmati

2013-01-01

347

Using the Internet in Middle Schools: A Model for Success. A Collaborative Effort between Los Alamos National Laboratory (LANL) and Los Alamos Middle School (LAMS).  

ERIC Educational Resources Information Center

Los Alamos National Laboratory (LANL) developed a model for school networking using Los Alamos Middle School as a testbed. The project was a collaborative effort between the school and the laboratory. The school secured administrative funding for hardware and software; and LANL provided the network architecture, installation, consulting, and…

Addessio, Barbara K.; And Others

348

Chromosome pairing of individual genomes in tall fescue (Festuca arundinacea Schreb.), its progenitors, and hybrids with Italian ryegrass (Lolium multiflorum Lam.).  

PubMed

A diploid-like pairing system prevents meiotic irregularities and improves the efficiency of gamete production in allopolyploid species. While the nature of the system is known in some polyploid crops including wheat, little is known about the control of chromosome pairing in polyploid fescues (Festuca spp.). In this work we studied chromosome pairing in allohexaploid F. arundinacea, its progenitors F. pratensis and F. glaucescens, and two intergeneric hybrids Lolium multiflorum (2x) x F. arundinacea (6x) and L. multiflorum (4x) x F. glaucescens (4x). The use of genomic in situ hybridization (GISH) permitted the analysis of homoeologous chromosome pairing and recombination of different genomes involved. We detected a diploid-like pairing system in polyploid fescues F. arundinacea and F. glaucescens, the latter being one of the progenitors of F. arundinacea. The pairing control system was absent in the second progenitor F. pratensis. Detailed analysis of intergeneric hybrids confirmed the presumed haploinsufficiency of the fescue system, which resulted in homoeologous pairing between all component genomes. This indicates that introgression of any specific chromosome segment from one genome to another is possible in all genome combinations. Our results not only contribute to the quest to discover the nature of the system controlling chromosome pairing in polyploid fescues, but may also have serious implications for design of hybrid breeding schemes in forage grasses. PMID:19420930

Kopecký, D; Bartos, J; Zwierzykowski, Z; Dolezel, J

2009-01-01

349

Using a Caesalpinia echinata Lam. protease inhibitor as a tool for studying the roles of neutrophil elastase, cathepsin G and proteinase 3 in pulmonary edema.  

PubMed

Acute lung injury (ALI) is characterized by neutrophil infiltration and the release of proteases, mainly elastase (NE), cathepsin G (Cat G) and proteinase 3 (PR3), which can be controlled by specific endogenous inhibitors. However, inhibitors of these proteases have been isolated from different sources, including plants. For this study, CeEI, or Caesalpinia echinata elastase inhibitor, was purified from C. echinata (Brazil-wood) seeds after acetone fractionation, followed by ion exchange and reversed phase chromatographic steps. Characterization with SDS-PAGE, stability assays, amino acid sequencing and alignment with other protein sequences confirmed that CeEI is a member of the soybean Kunitz trypsin inhibitor family. Like other members of this family, CeEI is a 20 kDa monomeric protein; it is stable within a large pH and temperature range, with four cysteine residues forming two disulfide bridges, conserved amino acid residues and leucine-isoleucine residues in the reactive site. CeEI was able to inhibit NE and Cat G at a nanomolar range (with K(i)s of 1.9 and 3.6 nM, respectively) and inhibited PR3 within a micromolar range (K(i) 3.7 ?M), leading to hydrolysis of specific synthetic substrates. In a lung edema model, CeEI reduced the lung weight and pulmonary artery pressure until 180 min after the injection of zymosan-activated polymorphonuclear neutrophils. In experiments performed in the presence of a Cat G and PR3, but not an NE inhibitor, lung edema was reduced only until 150 min and pulmonary artery pressure was similar to that of the control. These results confirm that NE action is crucial to edema establishment and progression. Additionally, CeEI appears to be a useful tool for studying the physiology of pulmonary edema and provides a template for molecular engineering and drug design for ALI therapy. PMID:24140156

Cruz-Silva, Ilana; Neuhof, Christiane; Gozzo, Andrezza Justino; Nunes, Viviane Abreu; Hirata, Izaura Yoshico; Sampaio, Misako Uemura; Figueiredo-Ribeiro, Rita de Cássia; Neuhof, Heinz; Araújo, Mariana da Silva

2013-12-01

350

Germinação de sementes e desenvolvimento de plântulas de moringa (Moringa oleifera Lam.) em função do peso da semente e do tipo de substrato  

Microsoft Academic Search

Germination of seeds and seedling development of drumstick as a function of seed weight and substrate type To compensate the shortage of information on the influence of seed weight and substrate over the germination and seedling development of Moringa oleifera an experiment was conducted under greenhouse conditions (50% of natural light with intermittent nebulization) in Fortaleza, Ceará State, Brazil. The

Antonio Marcos E. Bezerra; Valéria G. Momenté; Sebastião Medeiros Filho

2004-01-01

351

Moringa oleifera Lam. leaf extract prevents early liver injury and restores antioxidant status in mice fed with high-fat diet.  

PubMed

Consumption of high-fat diet (HFD) induces nonalcoholic fatty liver disease (NAFLD) and may lead to multiple complications affecting human health. In the present study, effect of Moringa oleifera leaf extract (MoLE) in alleviating HFD induced liver injury in mice has been reported. Liver histology and serum activity of hepatic marker enzymes i.e. aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) have been studied. Lipid peroxidation (LPO), ferric reducing antioxidant power (FRAP) and reduced glutathione (GSH) were also estimated using liver homogenate. Results of the study suggested that MoLE treatment protected HFD-induced liver damage as indicated by histopathology and liver enzyme activity compared to only-HFD fed group (P < 0.05). Interestingly, early signs of HFD-induced fatty liver were also alleviated by MoLE. Moreover, significant increase in endogenous antioxidant parameters and lower lipid peroxidation were found in liver of all MoLE treated groups. Results of the study indicated that MoLE has both preventive as also curative hepatoprotective activity. PMID:22734251

Das, Nilanjan; Sikder, Kunal; Ghosh, Santinath; Fromenty, Bernard; Dey, Sanjit

2012-06-01

352

Please forward this completed form to Irene Lam, 2.6054, for inclusion in employee personnel file. Rev. 11/14/11  

E-print Network

in employee personnel file. Rev. 11/14/11 Employee Separation-Checklist Employee: Last Day Worked: Forwarding Address: City: State: Zip: Supervisor: Employee of Resignation/Retirement o Letter of Resignation/Retirement o Retirement Checklist (if

Lightsey, Glenn

353

Overexpression of horsegram (Macrotyloma uniflorum Lam.Verdc.) NAC transcriptional factor (MuNAC4) in groundnut confers enhanced drought tolerance.  

PubMed

The NAC family being the largest plant-specific transcription factors functions in diverse and vital physiological processes during development. NAC proteins are known to be crucial in imparting tolerance to plants against abiotic stresses, such as drought and salinity, but the functions of most of them are still elusive. In this study, we report for the first time expression of the MuNAC4, a member of NAC transcription factor from horsegram (Macrotyloma uniflorum) conferring drought tolerance. The groundnut (Arachis hypogaea) transgenics were generated using recombinant MuNAC4 binary vector transformation approach. Molecular analysis of these transgenic lines confirmed the stable gene integration and expression of the MuNAC4 gene. Twelve lines of T5 generation exhibited significantly enhanced tolerance to drought stress with proliferated lateral root growth as compared to wild types. Transgenics exposed to long-term desiccation stress assays showed increased lateral roots and greenish growth. The physiological parameters analysis also suggests that overexpression of MuNAC4 plays a significant role in improving the water stress tolerance of transgenic groundnut, reducing the damage to membrane structures and enhancing osmotic adjustment and antioxidative enzyme regulation under stress. This study validates MuNAC4 as an important candidate gene for future phytoengineering approaches for drought tolerance in crop plants. PMID:24748414

Pandurangaiah, Merum; Lokanadha Rao, G; Sudhakarbabu, O; Nareshkumar, A; Kiranmai, K; Lokesh, U; Thapa, Ganesh; Sudhakar, Chinta

2014-08-01

354

Microbial growth and nitrogen immobilization in the root zone of barley ( Hordeum vulgare L.), Italian ryegrass ( Lolium multiflorum Lam.), and white clover ( Trifolium repens L.)  

Microsoft Academic Search

The effect of roots on microbial growth and N immobilization was investigated in a pot experiment with barley, Italian ryegrass, and white clover. We used a silty subsoil with a low soil organic matter content (0.16%C and 0.012%N), which allowed us to measure N immobilization as an increase in total soil organic N (planted versus unplanted). At sampling, the soil

T. A. Breland; L. R. Bakken

1991-01-01

355

Development of simple sequence repeat (SSR) markers and construction of an SSR-based linkage map in Italian ryegrass ( Lolium multiflorum Lam.)  

Microsoft Academic Search

In order to develop simple sequence repeat (SSR) markers in Italian ryegrass, we constructed a genomic library enriched for (CA)n-containing SSR repeats. A total of 1,544 clones were sequenced, of which 1,044 (67.6%) contained SSR motifs, and 395 unique clones were chosen for primer design. Three hundred and fifty-seven of these clones amplified products of the expected size in both

Mariko Hirata; Hongwei Cai; Maiko Inoue; Nana Yuyama; Yuichi Miura; Toshinori Komatsu; Tadashi Takamizo; Masahiro Fujimori

2006-01-01

356

A Nonhost Peptidase Inhibitor of ~14?kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera.  

PubMed

Helicoverpa armigera is one of the major devastating pests of crop plants. In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H. armigera larvae. B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14?kDa with or without reduction. In vitro studies towards total gut proteolytic enzymes of H. armigera and bovine trypsin indicated measurable inhibitory activity. B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively. The IC50 of B. monosperma peptidase inhibitor against total H. armigera gut proteinases activity was 2.0?µg/mL. The larval feeding assays suggested B. monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H. armigera. Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced. The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H. armigera suggest its usefulness in insect pest management of food crops. PMID:24860667

Pandey, Prabhash K; Singh, Dushyant; Singh, Sangram; Khan, M Y; Jamal, Farrukh

2014-01-01

357

Analysis of multiple biomarkers shows that lymphoma-associated macrophage (LAM) content is an independent predictor of survival in follicular lymphoma (FL)  

Microsoft Academic Search

We studied the role of multiple biomark- ers in determining outcome in follicular lymphoma (FL), concentrating in particu- lar on the role of benign macrophages. The study group consisted of uniformly staged and treated patients with FL en- rolled in a phase 2 trial between 1987 and 1993. All patients were younger than 61 years of age, had advanced-stage FL,

Pedro Farinha; Hamid Masoudi; Brian F. Skinnider; Karey Shumansky; John J. Spinelli; Karamjit Gill; Richard Klasa; Nicolas Voss; Joseph M. Connors; Randy D. Gascoyne

2005-01-01

358

Facile Synthesis of a Tungsten Alkylidyne Catalyst for Alkyne Zachary J. Tonzetich, Yan Choi Lam, Peter Muller, and Richard R. Schrock*  

E-print Network

ligands reside above the CalkylOO face, while the tert-butyl group of the neopentyl ligand resides below-Bu)(OAr)2 in 40-50% isolated yield. W(C-t-Bu)(CH2-t-Bu)(OAr)2 is a Versatile entry for preparing W), followed by displacement of the chlorides with alkoxides.8 The key synthesis of volatile, yellow

Müller, Peter

359

The movement of air in and out of the lungs is accom-plished by the contraction of voluntary muscles (Lam-  

E-print Network

-cord occlusion alone produced a high attrition rate. Compression com- bined with umbilical-cord occlusion or umbilical-cord occlusion, significantly increased fetal breathing rate. In contrast, umbilical ameliorated this out- 261 Copyright 1995 Psychonomic Society, Inc. This research was supported by National

360

A Secure Image Transfer Application for the NHSnet Kerry Jean kjean@ee.ucl.ac.uk, Walter Eaves weaves@ee.ucl.ac.uk, John Lam  

E-print Network

of the security technologies investigated in the HARP (Harmonisation for the security of web technologies and a according to the implemented policy [1]. The HARP (HArmonisation for the secuRity of web technologies and a, their exploitation in telemedicine introduces many security concerns. Through the use of secure servers called HARP

Haddadi, Hamed

361

Parsing dimensional versus diagnostic category-related patterns of reward circuitry function in behaviorally and emotionally dysregulated youth in the Longitudinal Assessment of Manic Symptoms (LAMS) study  

PubMed Central

Context Pediatric disorders characterized by behavioral and emotional dysregulation pose diagnostic and treatment challenges because of high comorbidity, suggesting that they may be better conceptualized dimensionally rather than categorically. Identifying neuroimaging measures associated with behavioral and emotional dysregulation in youth may inform understanding of underlying dimensional vs. disorder-specific pathophysiology. Objective Identify, in a large cohort of behaviorally and emotionally dysregulated youth, neuroimaging measures that: 1) are associated with behavioral and emotional dysregulation pathological dimensions (behavioral and emotional dysregulation measured with the Parent General Behavior Inventory 10 Item Mania Scale [PGBI-10M], mania, depression, anxiety); or 2) differentiate diagnostic categories(BPSD, ADHD, anxiety, disruptive behavior disorders (DBD)). Design Multi-site neuroimaging study(February 2011–April 2012). Setting Academic medical centers: Case Western Reserve University, Cincinnati Children’s Hospital, University of Pittsburgh. Patients Referred sample of behaviorally and emotionally dysregulated youth(n=85) from the Longitudinal Assessment of Manic Symptoms study and healthy youth (n=20). Main Outcome Measures Region-of-interest analyses examined relationships among prefrontal-ventral striatal reward circuitry during a reward paradigm (Win, Loss, control conditions), symptom dimensions, and diagnostic categories. Results Regardless of diagnosis, higher PGBI-10M scores were associated with greater left middle prefrontal cortical (mPFC; r=0.28), and greater levels of anxiety with greater right dorsal anterior cingulate cortical (dACC; r=0.27), activity to Win. The 20 highest (t=2.75) and 20 lowest (t=2.42) PGBI-10M scoring youth showed significantly greater left mPFC activity to Win than 20 healthy youth. DBD were associated with lower left ventrolateral prefrontal cortex(VLPFC) activity to Win (t=2.68) (all ps<0.05, corrected). Conclusions Greater PGBI-10M-related left mPFC activity, and greater anxiety-related right dACC activity, to Win may reflect heightened reward sensitivity and greater attention to reward in behaviorally and emotionally dysregulated youth, regardless of diagnosis. Reduced left VLPFC activity to Win may reflect reward insensitivity in youth with DBD. Despite a distinct reward-related neurophysiology in DBD, findings generally support a dimensional approach to studying neural mechanisms in behaviorally and emotionally dysregulated youth. PMID:24285346

Bebko, Genna; Bertocci, Michele A.; Fournier, Jay C.; Hinze, Amanda K.; Bonar, Lisa; Almeida, Jorge R. C.; Perlman, Susan B.; Versace, Amelia; Schirda, Claudiu; Travis, Michael; Gill, Mary Kay; Demeter, Christine; Diwadkar, Vaibhav A.; Ciuffetelli, Gary; Rodriguez, Eric; Olino, Thomas; Forbes, Erika; Sunshine, Jeffrey L.; Holland, Scott K; Kowatch, Robert. A.; Birmaher, Boris; Axelson, David; Horwitz, Sarah M.; Arnold, L. Eugene; Fristad, Mary. A; Youngstrom, Eric A.; Findling, Robert L.; Phillips, Mary L.

2014-01-01

362

Atividades melanogÃnica, genotÃxica e antiproliferativa de extratos de Brosimum gaudichaudii TrÃcul e Dorstenia brasiliensis Lam induzidas por radiaÃÃo UVA.  

E-print Network

??As furanocumarinas lineares ou psoralenos sÃo fotossensibilizadores empregados na dermatologia associados a ultravioleta A no tratamento de doenÃas como o vitiligo. A fotoquimioterapia tem reaÃÃes… (more)

Adriana Elias Pires Quevedo

2011-01-01

363

Arbuscular mycorrhizal fungi associated with Artemisia umbelliformis Lam, an endangered aromatic species in Southern French Alps, influence plant P and essential oil contents.  

PubMed

Root colonization by arbuscular mycorrhizal (AM) fungi of Artemisia umbelliformis, investigated in natural and cultivated sites in the Southern Alps of France, showed typical structures (arbuscules, vesicles, hyphae) as well as spores and mycelia in its rhizosphere. Several native AM fungi belonging to different Glomeromycota genera were identified as colonizers of A. umbelliformis roots, including Glomus tenue, Glomus intraradices, G. claroideum/etunicatum and a new Acaulospora species. The use of the highly mycorrhizal species Trifolium pratense as a companion plant impacted positively on mycorrhizal colonization of A. umbelliformis under greenhouse conditions. The symbiotic performance of an alpine microbial community including native AM fungi used as inoculum on A. umbelliformis was evaluated in greenhouse conditions by comparison with mycorrhizal responses of two other alpine Artemisia species, Artemisia glacialis and Artemisia genipi Weber. Contrary to A. genipi Weber, both A. umbelliformis and A. glacialis showed a significant increase of P concentration in shoots. Volatile components were analyzed by GC-MS in shoots of A. umbelliformis 6 months after inoculation. The alpine microbial inoculum increased significantly the percentage of E-?-ocimene and reduced those of E-2-decenal and (E,E)-2-4-decadienal indicating an influence of alpine microbial inoculum on essential oil production. This work provides practical indications for the use of native AM fungi for A. umbelliformis field culture. PMID:21243378

Binet, Marie-Noëlle; van Tuinen, Diederik; Deprêtre, Nicolas; Koszela, Nathalie; Chambon, Catherine; Gianinazzi, Silvio

2011-08-01

364

Evaluation of nutritional quality of moringa ( Moringa oleifera Lam.) leaves as an alternative protein source for Nile tilapia ( Oreochromis niloticus L.)  

Microsoft Academic Search

This study was conducted to evaluate the suitability of freeze-dried moringa leaf meal, Moringa oleifera, as an alternative protein source for Nile tilapia. Three experimental diets were formulated to contain moringa leaf meal at levels of 10%, 20% and 30% of the total dietary protein (Diets 2, 3 and 4, respectively) and one diet acting as a control (Diet 1)

Nahid Richter; Perumal Siddhuraju; Klaus Becker

2003-01-01

365

Influence of forest structure and environmental variables on recruit survival and performance of two Mediterranean tree species ( Quercus faginea L. and Q. suber Lam.)  

Microsoft Academic Search

We investigated the regeneration requirements of the two dominant tree species in a mixed-oak forest of SW Portugal: Quercus suber (cork oak, evergreen) and Q.\\u000a faginea (Portuguese oak, winter-deciduous). We hypothesized that (1) recruits of different oak species are differentially influenced\\u000a by soil and overstory variables and (2) different factors explain the recruitment occurrence and performance of the same species.

Sara Maltez-Mouro; Luis V. García; Helena Freitas

2009-01-01

366

Croissance du trfle violet (Trifolium pratense L.) en association avec du ray-grass d'Italie  

E-print Network

'Italie (Lolium multiflorum Lam. ssp. italicum). I. - Description de l'organisation morphologique du trèfle (1 with Italian rye-grass (Lolium multiflorum Lam. spp. italicum). I. — Morphological organization

Boyer, Edmond

367

Croissance du trfle violet (Trifolium pratense L.) en association avec du ray-grass d'Italie  

E-print Network

'Italie (Lolium multiflorum Lam. ssp. italicum) : II. - Démographie des organes, biomasse et interférences pratense L.) with Italian rye-grass (Lolium multiflorum Lam. ssp. italicum) : 11 Demography of organs

Paris-Sud XI, Université de

368

California Energy Commission STAFF REPORT  

E-print Network

Graillat Helen Lam Samuel Lerman Rebecca Menten G. William Pennington Justin Regnier Keith Roberts Jenny Wu, Devorah Eden, Todd Ferris, Chris Graillat, Helen Lam, Samuel Lerman, Rebecca Menten, Bill Pennington

369

108 Natural Areas Journal Volume 22 (2), 2002 Disturbance  

E-print Network

will be dominated by hardwoods such as black oak (Quercus velutina Lam.), white oak (Quercus alba L.), and hickories bosque probablemente esté dominada por maderas duras, tales como Quercus velutina Lam., Quercus alba L

Stambaugh, Michael C

370

Characterization of a Truncated Lipoarabinomannan from the Actinomycete Turicella otitidis  

Microsoft Academic Search

Lipoarabinomannan (LAM) lipoglycans have been characterized from a range of mycolic acid-containing actinomycetes and from the amycolate actinomycete Amycolatopsis sulphurea. To further understand the struc- tural diversity of this family, we have characterized the lipoglycan of the otic commensal Turicella otitidis. T. otitidis LAM (TotLAM) has been determined to consist of a mannosyl phosphatidylinositol anchor unit carrying an ( 136)-linked

Martine Gilleron; Natalie J. Garton; Jerome Nigou; Therese Brando; Germain Puzo; Iain C. Sutcliffe

2005-01-01

371

Original article Development of SCARs useful for species  

E-print Network

. multiflorum Lam. / Lolium perenne L. / RAPD marker / SCAR Résumé - Développement de SCAR pour l hasard) sur deux variétés de Lolium multiflorum Lam. et quatre de Lolium perenne L., dans le but de of agronomical- ly important temperate forage grass species. Lolium perenne L. and L. multiflorum Lam. are two

Paris-Sud XI, Université de

372

Laser Additive Manufacturing and Bionics: Redefining Lightweight Design  

Microsoft Academic Search

New layer wise manufacturing technologies such as Laser Additive Manufacturing (LAM) allow innovative approaches to product design. Especially for lightweight design in aircraft applications LAM offers new possibilities for load-adapted structures. However, to fully capture lightweight potential of LAM technologies new design guidelines and processes have to be developed. A novel approach to extreme lightweight design is realized by incorporating

C. Emmelmann; P. Sander; J. Kranz; E. Wycisk

2011-01-01

373

Biophysical Journal, Volume 99 Supporting Material  

E-print Network

is ~.06 ejections/sec (at 1:500,000 SYBR gold dilution). The rate of ejections is substantially increased ejections without LamB The rate of ejections without LamB is ~ 0.008 ejections/sec; with LamB added the rate blocking agents (BSA, casein, heparin, as detailed in the Materials and Methods) and saw the same rate

Phillips, Rob

374

Leaf dynamics, stemflow and throughfall water and nutrient inputs in a subtropical savanna parkland, Texas  

E-print Network

Deciduous Coriaceous Deciduous/ Goriacecus everrIreen Smooth Tree oz' ~; 1, 3 rounded crown Smooth Shrub or tree 1, 3 Smooth Small tree 1, 3 or large shrub 1, 3 Spiny ha~ (~Mlt' ll'da Il Agarito (Berberis trifoliolata Moric. ) Deciduous... rn 20 n In 2 400 BERBERIS DI 0 8PYR0 8 ZANTHOXYLUM LSD sso[ . : LSD~ 80 m 40 rn r Z 80 rn 40 g 60 E E ~ 40 IL IL 20 HAIL STORM o ~ * 's oo 60 E 40 E I- IL 20 HAIL STORM o t J S N J J S N J 1987 1988 1989 DATE J...

Angerer, Jay Peter

2012-06-07

375

The annual behavioral cycle of male white-tailed deer  

E-print Network

of total ground cover that they contribute in sech type (after goo4. 1971). Species Ac?era faro?siss?(L. )villd. A. ~wzdula Beuth. A. tortucsa(L. )villa. Berberis trifoliolats Noric Buc lia ~1?n inosa Per?. Ct'. itis I~art a s V1114, C. reticl 'ara... of total ground cover that they contribute in sech type (after goo4. 1971). Species Ac?era faro?siss?(L. )villd. A. ~wzdula Beuth. A. tortucsa(L. )villa. Berberis trifoliolats Noric Buc lia ~1?n inosa Per?. Ct'. itis I~art a s V1114, C. reticl 'ara...

Brown, Bennett A

2012-06-07

376

Energy E#cient Online Deadline Scheduling H.L. Chan # W.T. Chan # T.W. Lam # L.K. Lee # K.S. Mak # P. Wong +  

E-print Network

the energy to complete all jobs; O(1)­competitive online algorithms with respect to energy usage have been of work in one unit of time. Jobs arrive online at unpredictable times; the work and deadline of a job are known when the job arrives. The aim is to design an (online) algorithm that maximizes the throughput

Lam, Tak-Wah

377

Effects of dehydrated methanol extracts of moringa ( Moringa oleifera Lam.) leaves and three of its fractions on growth performance and feed nutrient assimilation in Nile tilapia ( Oreochromis niloticus (L.))  

Microsoft Academic Search

A 10-week feeding trial was conducted in a recirculation system at (27±0.5 °C) to determine the effect of a methanol extract of moringa leaf meal and its different fractions. Nine isonitrogenous and isoenergetic diets containing 35% crude protein and 20 MJ kg?1 gross energy were used. All the experimental diets contained the same amount of fish meal. The inclusion of moringa methanol

Euloge Dongmeza; Perumal Siddhuraju; George Francis; Klaus Becker

2006-01-01

378

Abortion and determination of stages for embryo rescue in crosses between sweet-potato, Ipomoea batatas Lam. (2n=6x=90) and its wild relative, I. trifida (H. B. K.) G. Don. (2n=2x=30)  

Microsoft Academic Search

Summary  The frequency of aborted fruits and the changes and abnormalities that occur during the embryo development in intraspecific crosses of sweet-potato Ipomoea batatas (2n=6x=90) and interspecific crosses between I. batatas and I. trifida (2n=2x=30) were investigated in order to study the causes of the low seed production. Three genotypes of I. batatas and 18 genotypes of I. trifida were intermated.

José Mont; Masaru Iwanaga; Gisella Orjeda; Kazuo Watanabe

1993-01-01

379

Harland, C.M., Zheng, J., Johnsen. T. and Lamming. R.C. (forthcoming). A Conceptual Model for Researching the Creation and Operation of Supply Networks. Accepted for publication in the British  

E-print Network

for Researching the Creation and Operation of Supply Networks. Accepted for publication in the British Journal in "British Journal of Management 15, 1 (2004) 1-21" DOI : 10.1111/j.1467-8551.2004.t01-1-00397.x #12;Harland the Creation and Operation of Supply Networks. Accepted for publication in the British Journal of Management

Paris-Sud XI, Université de

380

Electrokinetic detection for X-ray spectra of weakly interacting liquids: n-decane and n-Royce K. Lam, Orion Shih, Jacob W. Smith, Alex T. Sheardy, Anthony M. Rizzuto, David Prendergast, and  

E-print Network

liquid surfaces by synchrotron x-ray spectroscopy of liquid microjets Rev. Sci. Instrum. 75, 725 (2004Electrokinetic detection for X-ray spectra of weakly interacting liquids: n-decane and n- nonane. Lett. 87, 182908 (2005); 10.1063/1.2126112 X-ray absorption spectra of water within a plane-wave Car

Cohen, Ronald C.

381

Sulfated modification and anti-tumor activity of laminarin  

PubMed Central

The aim of this study was to investigate the sulfated modification of laminarin and the changes in structure and antitumor activity. The chlorosulfonic acid-pyridine method was applied for sulfated modification. The molecular weights of laminarin and laminarin sulfate (LAMS) were measured by high-performance liquid chromatography (HPLC), and IR and NMR spectra were also recorded. The surface conformations of laminarin and LAMS were observed with a scanning electron microscope. The antitumor activities of the two polysaccharides were also evaluated using an MTT assay. LAMS with a sulfate content of 45.92% and a molecular weight of 16,000 was synthesized. The IR spectra of laminarin and LAMS showed the characteristic absorption peaks of a polysaccharide, and LAMS also had the characteristic absorption peaks of sulfate moieties. The NMR spectra showed that laminarin and LAMS had ?-(1?3) glycosidic bonds forming the main chain, and sulfate substitution was at the hydroxyl groups of C2 and C6. Under the scanning electron microscope, there were clear differences in surface conformation between laminarin and LAMS; laminarin was cloud-like and spongy, while LAMS was block-like and flaky. The MTT results showed that laminarin and LAMS had inhibitory effects on LoVo cell growth, and the antitumor activity of LAMS was higher than that of laminarin at the same concentration. This suggests that sulfated modification was able to change the laminarin structure and markedly enhance the antitumor activity. PMID:24223655

JI, CHEN-FENG; JI, YU-BIN; MENG, DE-YOU

2013-01-01

382

Doxycycline inhibits matrix metalloproteinase-2 secretion from TSC2-null mouse embryonic fibroblasts and lymphangioleiomyomatosis cells  

PubMed Central

BACKGROUND AND PURPOSE Lymphangioleiomyomatosis (LAM) is characterized by the abnormal growth of smooth muscle-like cells (LAM cells) and cystic destruction of the lung parenchyma. LAM cell-derived matrix metalloproteinases (MMPs) are thought to play a prominent role in the tissue destruction. The aim of this study was to determine whether doxycycline, a known MMP inhibitor, can inhibit LAM cell proliferation or mitochondrial function and/or modulate MMPs and their tissue inhibitors (TIMPs). EXPERIMENTAL APPROACH Wild-type and tuberous sclerosis complex-2 (TSC2)-null mouse embryonic fibroblasts (MEFs) were cultured in DMEM containing 10% fetal bovine serum (FBS). Human LAM cells were derived from the lungs of LAM patients and airway smooth muscle cells from control subjects. Cells were stimulated with FBS with or without doxycycline for up to 9 days. Proliferation was assessed by manual cell counts and MTT assay, MMP production by zymography and ELISA, and TIMP production using elisa. KEY RESULTS Doxycycline did not change FBS-induced proliferation in MEFs or human cells. However, doxycycline did reduce metabolic activity of both wild-type and TSC2-null MEFs and LAM cells, but had no effect on control cells. Furthermore, doxycycline reduced MMP-2 from MEFs and decreased active-MMP-2 from LAM cells but had no effect on TIMP-1 and TIMP-2 from human LAM cells. CONCLUSIONS AND IMPLICATIONS Doxycycline decreased MMP levels and cell metabolic activity, which raises the possibility of therapeutic efficacy in LAM. PMID:21418186

Moir, LM; Ng, HY; Poniris, MH; Santa, T; Burgess, JK; Oliver, BGG; Krymskaya, VP; Black, JL

2011-01-01

383

Positioning ganglioside D3 as an immunotherapeutic target in lymphangioleiomyomatosis.  

PubMed

Tumors that develop in lymphangioleiomyomatosis (LAM) as a consequence of biallelic loss of TSC1 or TSC2 gene function express melanoma differentiation antigens. However, the percentage of LAM cells expressing these melanosomal antigens is limited. Here, we report the overexpression of ganglioside D3 (GD3) in LAM. GD3 is a tumor-associated antigen otherwise found in melanoma and neuroendocrine tumors; normal expression is largely restricted to neuronal cells in the brain. We also observed markedly reduced serum antibody titers to GD3, which may allow for a population of GD3-expressing LAM cells to expand within patients. This is supported by the demonstrated sensitivity of cultured LAM cells to complement mediated cytotoxicity via GD3 antibodies. GD3 can serve as a natural killer T (NKT) cell antigen when presented on CD1d molecules expressed on professional antigen-presenting cells. Although CD1d-expressing monocyte derivatives were present in situ, enhanced NKT-cell recruitment to LAM lung was not observed. Cultured LAM cells retained surface expression of GD3 over several passages and also expressed CD1d, implying that infiltrating NKT cells can be directly cytotoxic toward LAM lung lesions. Immunization with antibodies to GD3 may thus be therapeutic in LAM, and enhancement of existing NKT-cell infiltration may be effective to further improve antitumor responses. Overall, we hereby establish GD3 as a suitable target for immunotherapy of LAM. PMID:23665200

Gilbert, Emily R; Eby, Jonathan M; Hammer, Adam M; Klarquist, Jared; Christensen, David G; Barfuss, Allison J; Boissy, Raymond E; Picken, Maria M; Love, Robert B; Dilling, Daniel F; Le Poole, I Caroline

2013-07-01

384

Lymphangioleiomyomatosis -- a wolf in sheep's clothing  

PubMed Central

Lymphangioleiomyomatosis (LAM) is a rare progressive lung disease of women. LAM is caused by mutations in the tuberous sclerosis genes, resulting in activation of the mTOR complex 1 signaling network. Over the past 11 years, there has been remarkable progress in the understanding of LAM and rapid translation of this knowledge to an effective therapy. LAM pathogenic mechanisms mirror those of many forms of human cancer, including mutation, metabolic reprogramming, inappropriate growth and survival, metastasis via blood and lymphatic circulation, infiltration/invasion, sex steroid sensitivity, and local and remote tissue destruction. However, the smooth muscle cell that metastasizes, infiltrates, and destroys the lung in LAM arises from an unknown source and has an innocent histological appearance, with little evidence of proliferation. Thus, LAM is as an elegant, monogenic model of neoplasia, defying categorization as either benign or malignant. PMID:23114603

Henske, Elizabeth P.; McCormack, Francis X.

2012-01-01

385

Bacterial Source Tracking to Support the Development and Implementation of Watershed Protection Plans for the Lampasas and Leon Rivers: Lampasas River Watershed Final Report  

E-print Network

in this report as 0.5 inches, or more, per day). Precipitation between February 1 and September 30, 2011 averaged 4.33 inches. Precipitation between October 1, 2011 and January 24, 2012 averaged 7.34 inches. No flow condition (ponded) in Reese Creek....63 5.97 7.23 2015 1.27 LAM 7 15781 19.33 7.59 7.84 2406 11.61 LAM 8 21015 18.66 8.60 7.80 416 0.12 LAM 9 15250 18.15 8.30 7.82 2107 12.89 LAM 10 11897 21.34 11.10 8.27 1996 25.53 LAM 11 16404 22.30 9.40 7.91 496 17.82 LAM 12 11724 17.88 8.08 7...

Gregory, L.; Casarez, E.; Truesdale, J.; Di Giovanni, G.; Owen, T; Wolfe, J.

2013-04-25

386

Notes on Erysiphales (Ascomycetes) from Patagonia, Argentina  

Microsoft Academic Search

Fifteen Erysiphaceous taxa found on 20 host plant species in Patagonia are documented. A new species Oidium maculatae (type host: Viola maculata) is described. Berberis linearifolia, Buddleja globosa, Prosopis alpataco and Viola maculata, are new host plants for Erysiphales. Three new combinations on fungi and host plant species were founded: Erysiphe howeana – Fuchsia magellanica; E. patagoniaca – Nothofagus pumilio

María Havrylenko; Susumu Takamatsu

2005-01-01

387

IR-4 Ornamental Horticulture Program Research Report Form  

E-print Network

on Abelia at any of the tested levels. Plant Growth. While the variability in height and width changes, Figure 3a). Mesotrione caused growth inhibition for Abelia. Berberis Phytotoxicity and Plant Growth that the trial should be considered inconclusive. Plant Growth. The plants grew in height and width over

Lieth, J. Heinrich

388

Influence of egg depth in host plants on parasitism of Scolylbopa australis (Homoptera: Ricaniidae) by Centrodora scolypopae (Hymenoptera: Aphelinidae)  

Microsoft Academic Search

Percentage parasitism of the eggs of the passion vine hopper (Scolypopa australis) by Centrodora scolypopae varies with the host plant. The depth of the S. australis eggs below the surface was measured on Pteridium aquilinum var esculentum, Rubus fruticosus agg., Berberis darwinii, Polygonum convolvulus, Coriuria arborea and Cyathea sp., and related to the observed parasitism. It was found that egg

P. J. Gerard

1989-01-01

389

2009 Virginia Polytechnic Institute and State University 2901-1050 Virginia Cooperative Extension programs and employment are open to all, regardless of race, color, national origin, sex, religion, age, disability, political beliefs, sexual orientation, o  

E-print Network

, Administrator, 1890 Extension Program, Virginia State, Petersburg. Japanese Barberry (Berberis thunbergii var: Upright mound This medium to large shrub has purple foliage throughout the growing season. Japanese: Consult local garden centers, historic or public gardens and arboreta, regarding cultivars and related

Liskiewicz, Maciej

390

Now @ THE GARDENS, SUMMER 2006 7 THIS SPRING ROYAL BOTANICAL GARDENS' natural lands  

E-print Network

Now @ THE GARDENS, SUMMER 2006 7 THIS SPRING ROYAL BOTANICAL GARDENS' natural lands staff performed of The Gardens' tallgrass remnants -- prairies and savannahs -- patches of what is probably the most imperiled, the savannah is being heavily invaded by shrubs -- particularly Japanese barberry (Berberis thunbergii), black

391

Carbon Dioxide Exchange by Several Streamside and Scrub Oak Community Species of Red Butte Canyon, Utah  

Microsoft Academic Search

ABSTRACT: Species of a xeric scrub oak community (scrub oak, Quercus gambelii; sagebrush, Artemisia tridentata; bitterbrush, Purshia have lower seasonal water potentials than species of a mesic streamside habitat (box elder, Acer negundo; bigtooth maple, Acer grandidentatum; creeping barberry, Berberis repens). The restriction of box elder to mesic sites is, in part, a function of its physiological responses to moisture

Stephen J. Dina; Lionel G. Klikoff

2007-01-01

392

Evaluation of a large-scale invasive plant species herbicide control program in the Berkshire Taconic Plateau, Massachusetts, USA  

Microsoft Academic Search

SUMMARY In the late 1990's, The Nature Conservancy (TNC) documented a rise in five invasive plant species, barberry Berberis thunbergii , bittersweet Celastrus orbiculatus , garlic mustard Alliaria petiolata , buckthorn Frangula alnus , and honeysuckle Lonicera morrowii on the periphery of the relatively intact and uninvaded 14,600 ha Berkshire Taconic Plateau in Massachusetts (USA). The Plateau comprises an ecologically

Lindsay McAlpine; Stephen Porder

393

TABIENINA A: UN NUEVO ALCALOIDE APORFINABENCILISOQUINOLINA OXIDADO  

Microsoft Academic Search

Guided by Brine shrimp toxicity assay and Culex quinquefasciatus larvicide bioassay, fractionation of the alkaloidal extract from the stems of Berberis tabiensis (Berberidaceae) provided a novel oxidized aporphine- benzylisoquinoline alkaloid (tabienine A). The structure was determined by chemical methods and spectral analysis.

RODOLFO QUEVEDO; BÁRBARA MORENO M; VICTOR M. FAJARDO; MATERIALES Y MÉTODOS

394

INVASIVE SHRUBS AND SONGBIRD NESTING SUCCESS: EFFECTS OF CLIMATE VARIABILITY AND PREDATOR ABUNDANCE  

Microsoft Academic Search

Previous studies have demonstrated that songbirds often use exotic plants as nesting substrates and may suffer elevated predation rates relative to nests placed in native plants. Veeries (Catharus fuscescens) frequently build nests in an exotic shrub, Japanese barberry (Berberis thunbergii), in forests of southeastern New York State, USA. We monitored Veery nesting success over a six-year period and supplemented these

Kenneth A. Schmidt; Lisa C. Nelis; Nathan Briggs; Richard S. Ostfeld

2005-01-01

395

Differences in Earthworm Densities and Nitrogen Dynamics in Soils Under Exotic and Native Plant Species  

Microsoft Academic Search

Previous studies of the invasion of two exotic plants – Berberis thunbergii and Microstegium vimineum – in hardwood forests of New Jersey have shown a significant increase of pH in soils under the invasive plants as compared with soils from under native shrubs (Vaccinium spp). We present a further investigation of soil properties under the exotic plants in question. We

P. S. Kourtev; W. Z. Huang; J. G. Ehrenfeld

1999-01-01

396

IR-4 Ornamental Horticulture Program Research Report Form  

E-print Network

of the herbicide treatments (Tables 2 and 6a, Figure 2a). The herbicide V-10142 caused severe phytotoxicity and plant death for Abelia at all concentrations. This herbicide is not safe for weed control in Abelia of the extensive damage, there was little or no growth of Berberis plants in any of the herbicide treatments

Lieth, J. Heinrich

397

Growth response of native shrubs to acid mine spoil and to proposed soil amendments  

Microsoft Academic Search

Successful reclamation of acid mine sites may be enhanced by revegetating with species that are tolerant to acid mine spoil conditions. This study was conducted to assess the response of four native shrub species, Amelanchier alnifolia Nutt., Symphoricarpos albus (L.) Blake, Berberis repens Lindl., and Ceanothus sanguineus Pursh, to 1) pyritic acid mine spoil amended with various levels of lime

Pamela J. Voeller; Benjamin A. Zamora; James Harsh

1998-01-01

398

Potential of fungi for the biological control of some New Zealand weeds  

Microsoft Academic Search

The potential for fungi to control 14 important weeds in New Zealand is reviewed. Information provided includes the fungi already known from these weeds in New Zealand, fungi recorded from the native ranges of the weeds, and past work on the control of these weeds using fungi, both in New Zealand and elsewhere. The 14 weeds are: Berberis spp.; Buddleja

Peter R. Johnston

1990-01-01

399

The laser additive manufacture of Ti6Al4V  

Microsoft Academic Search

Laser additive manufacturing (LAM) is a manufacturing technique with cost-reduction potential for titanium aerospace components. The mechanical properties of LAM Ti-6Al-4V have been investigated extensively, but little work on microstructure evolution has been performed to date. The results presented here provide a first look at the relationships between LAM processing parameters and microstructure in as-deposited Ti-6Al-4V.

P. A. Kobryn; S. L. Semiatin

2001-01-01

400

The laser additive manufacture of Ti6Al4V  

Microsoft Academic Search

Laser additive manufacturing (LAM) is a manufacturing technique with cost-reduction potential for titanium aerospace components.\\u000a The mechanical properties of LAM Ti-6Al-4V have been investigated extensively, but little work on microstructure evolution\\u000a has been performed to date. The results presented here provide a first look at the relationships between LAM processing parameters\\u000a and microstructure in as-deposited Ti-6Al-4V.

P. A. Kobryn; S. L. Semiatin

2001-01-01

401

The Myth and the Mystery of Aja Nye  

E-print Network

to the western heavenly abode... Thirteen centuries after Guru Rinpoche made the above prophecy, a sacred nye (gnas) of Aja in Mongar still holds a mystical attraction for many Bhutanese pilgrims. This sacred nye is attributed to Guru Rinpoche who brought... in political affairs of Trashigang forced Sersanglam to leave for Tibet temporarily, while Aja Lam was assassinated at Jangphu. However, Sersanglam returned to Bhutan, perhaps after the place had become stable. The late Aja Lam's nephew Lam Sharchung stayed...

Wangchuk, Rinzin

2004-01-01

402

Detection of hepatitis B virus polymerase gene variants associated with Lamivudine, Adefovir and Entecavir resistance and some undefined mutations isolated from chronic hepatitis B patients in the south of Turkey  

Microsoft Academic Search

In order to detect the mutation patterns related to Lamivudine (LAM), Adefovir (ADV) and Entecavir (ETV) resistance, we examined\\u000a totally 230 stored HBsAg (+) and HBV DNA (+) sera samples of patients suffering chronic hepatitis B and treated with LAM,\\u000a ADV and ETV in the south of Turkey. 100, 110 and 20 sera were obtained from patients treated with LAM

Mehmet Sami Serin; Ebru Bekiroglu; Serpil Polat; Aylin Dogen; Seda Tezcan; Gonul Aslan; Orhan Sezgin; Engin Altintas; Gurol Emekdas; Enver Ucbilek

2010-01-01

403

Page 1 of 2 Last Updated: 09/17/13 Biomolecular Science and Engineering Graduate Degree Program  

E-print Network

University Kern Medical Center LAM Therapeutics Long Island University Marlboro College Massachusetts Southern Oregon University Southampton College Southwestern College Stanford University Sutro Biopharma Tek

Fygenson, Deborah Kuchnir

404

Initial report on the application of laser ablation - inductively coupled plasma mass spectrometry for the analysis of radioactive Hanford Tank Waste materials  

SciTech Connect

Initial LA/MS analyses of Hanford tank waste samples were performed successfully using laboratory and hot cell LA/MS instrumentation systems. The experiments described in this report have demonstrated that the LA/MS data can be used to provide rapid analysis of solid, radioactive Hanford tank waste samples to identify major, minor, and trace constituents (elemental and isotopic) and fission products and radioactive isotopes. The ability to determine isotopic constituents using the LA/MS method yielded significant advantages over ICP/AES analysis by providing valuable information on fission products and radioactive constituents.

Smith, M.R.; Hartman, J.S.; Alexander, M.L.; Mendoza, A.; Hirt, E.H.; Stewart, T.L.; Hansen, M.A.; Park, W.R.; Peters, T.J.; Burghard, B.J.

1996-12-01

405

IEEE TRANSACTIONS ON SEMICONDUCTOR MANUFACTURING, VOL. 15, NO. 4, NOVEMBER 2002 393 Real-Time Furnace Modeling and Diagnostics  

E-print Network

companies: Advanced Energy, ASML, Atmel Corporation, Advanced Micro Devices, Applied Materials, Asyst Technologies, Inc., BOC Edwards, Cymer, Etec Systems, Inc., Intel Corporation, KLA-TENCOR, Lam Research

California at Berkeley, University of

406

Ventilated Facade Design for Hot and Humid Climates  

E-print Network

of high-rise fully air-conditioned office buildings." Energy and Buildings, 26(2), 189-197. Lam, J. C., and Li, D. H. W. (1996). "Study of solar radiation data for Hong Kong." Energy Conversion and Management, 37(3), 343- 351. Lam, J. C., and Li, D... of high-rise fully air-conditioned office buildings." Energy and Buildings, 26(2), 189-197. Lam, J. C., and Li, D. H. W. (1996). "Study of solar radiation data for Hong Kong." Energy Conversion and Management, 37(3), 343- 351. Lam, J. C., and Li, D...

Haase, M.; Amato, A.

2006-01-01

407

La enhances IRES-mediated translation of laminin B1 during malignant epithelial to mesenchymal transition  

PubMed Central

The majority of transcripts that harbor an internal ribosome entry site (IRES) are involved in cancer development via corresponding proteins. A crucial event in tumor progression referred to as epithelial to mesenchymal transition (EMT) allows carcinoma cells to acquire invasive properties. The translational activation of the extracellular matrix component laminin B1 (LamB1) during EMT has been recently reported suggesting an IRES-mediated mechanism. In this study, the IRES activity of LamB1 was determined by independent bicistronic reporter assays. Strong evidences exclude an impact of cryptic promoter or splice sites on IRES-driven translation of LamB1. Furthermore, no other LamB1 mRNA species arising from alternative transcription start sites or polyadenylation signals were detected that account for its translational control. Mapping of the LamB1 5?-untranslated region (UTR) revealed the minimal LamB1 IRES motif between ?293 and ?1 upstream of the start codon. Notably, RNA affinity purification showed that the La protein interacts with the LamB1 IRES. This interaction and its regulation during EMT were confirmed by ribonucleoprotein immunoprecipitation. In addition, La was able to positively modulate LamB1 IRES translation. In summary, these data indicate that the LamB1 IRES is activated by binding to La which leads to translational upregulation during hepatocellular EMT. PMID:21896617

Petz, Michaela; Them, Nicole; Huber, Heidemarie; Beug, Hartmut; Mikulits, Wolfgang

2012-01-01

408

Horizontal and vertical movement of Pseudomonas fluorescens toward exudate of Macrophomina phaseolina in soil: influence of motility and soil properties.  

PubMed

The role of motility and cell surface hydrophobicity in transport and dispersal of Pseudomonas fluorescens strains LAM1-hydrophilic, LAM2-hydrophobic and LAM(NM) (non-motile mutant of LAM2) under different soil conditions was studied. Maximum adhesion was recorded for LAM2 in clay loam (70%), followed by sandy loam (68%) and sandy soil (40%). Vertical migration of P fluorescens isolates in soils was recorded at 5 and 25 cm flow of wafer or M. phaseolina exudate. In all the treatments, LAM1 exhibited maximum migration followed, by LAM2 and LAM(NM). The rate of migration of such isolates was lowered in water irrigated soils compared to those irrigated with M. phaseolina exudate. In sandy soil, cells of LAM1 migrated up to 13 cm in comparison to LAM2 (11 cm) and LAN(NM) (9 cm) at 5 cm flow of fungal exudate. Population of LAM1, LAM2 and LAM(NM) was 5.7, 5.68 and 5.61 log cfu g(-1) soil at 1 cm depth, but it decreased to 2.56, 2.21 and 1.99 log cfu during migration up to 11 cm in sandy soil at 5 cm flow of fungal exudate. Greater motility was observed in sandy soil irrigated with water or fungal exudate, followed by sandy loam and clay loam. In general, filtration coefficient (lambda) of P. fluorescens was higher in soils irrigated with 5 cm of water or exudate than with 25 cm of irrigation. The horizontal movement of P. fluorescens strains in sandy soil adjusted at different psi m showed marked reduction with decrease in psi m. The non-motile LAN(NM) did not show chemotactic response and migrated up to a maximum of 3 mm in saturated soils (0 kPa). After 96 h, LAM1 and LAM2 migrated upto 35 and 29 mm respectively in sandy soil. Motile isolates had significantly greater colonization of M. phaseolina sclerotia over the non-motile mutant. PMID:12002402

Singh, Tanuja; Srivastava, Alok K; Arora, Dilip K

2002-01-01

409

Effect of dietary laminarin and fucoidan on selected microbiota, intestinal morphology and immune status of the newly weaned pig.  

PubMed

A 2 × 2 factorial experiment was conducted to investigate the interactions between laminarin (LAM; 0 and 300 parts per million (ppm)) and fucoidan (FUC; 0 and 240 ppm) levels on intestinal morphology, selected microbiota and inflammatory cytokine gene expression in the weaned pig. There was an interaction between LAM and FUC supplementation on the Enterobacteriaceae population (P< 0·05) and the abundance of attaching and effacing Escherichia coli (AEEC) strains (P< 0·05) in the colon. Pigs offered the FUC diet had a reduced Enterobacteriaceae population compared with pigs offered the basal diet. However, the effect of FUC on the Enterobacteriaceae population was not observed when combined with LAM. Pigs offered the LAM diet had reduced abundance of AEEC strains compared with pigs offered the basal diet. However, there was no effect of LAM on the abundance of AEEC strains when combined with FUC. There was an interaction between LAM and FUC supplementation on villous height (P< 0·01) and the villous height:crypt depth ratio (P< 0·01) in the duodenum. Pigs offered the LAM or FUC diet had an increased villous height and villous height:crypt depth ratio compared with pigs offered the basal diet. However, there was no effect of the LAM and FUC combination diet on intestinal morphology. Pigs offered the LAM-supplemented diets had a lower IL-6 (P< 0·05), IL-17A (P< 0·01) and IL-1? (P< 0·01) mRNA expression in the colon compared with pigs offered the diets without LAM. In conclusion, supplementation with either LAM or FUC alone modified intestinal morphology and selected intestinal microbiota, but these effects were lost when offered in combination. PMID:23531383

Walsh, A M; Sweeney, T; O'Shea, C J; Doyle, D N; O'Doherty, J V

2013-11-14

410

Long-term outcomes of two rescue therapies in lamivudine-refractory patients with chronic hepatitis B: combined lamivudine and adefovir, and 1-mg entecavir  

PubMed Central

Background/Aims Adefovir (ADV) and lamivudine (LAM) combination therapy (ADV+LAM) has been a useful option for patients with LAM-resistant (LAM-r) chronic hepatitis B (CHB). However, the long-term outcomes of LAM+ADV and 1-mg entecavir (ETV) rescue therapies have still been limited. The aim of this study was to determine the long-term outcomes of these two rescue therapies. Methods Sixty patients with LAM-r CHB underwent rescue therapy with LAM+ADV (n=36) or 1-mg ETV (n=24). We determined the duration of rescue therapy, timing and type of mutation, undetectable serum hepatitis B virus (HBV) DNA by PCR (lower limitation of detection, < 140 copies/mL), biochemical response (alanine aminotransferase < 40 IU/mL), and the incidence of hepatitis B virus e antigen (HBeAg) seroconversion and virologic breakthrough. Results Baseline characteristics did not differ between the two therapy groups. The duration of rescue therapy was 56 months (range, 14-100 months) in the ADV+LAM group and 42 months (range, 12-73 months) in the ETV group (P=0.036). The cumulative rates of HBV DNA undetectability and HBeAg seroconversion up to 6 years were 88.6% and 43.0%, respectively, in the ADV+LAM group, and 45.8% and 31.8% in the ETV group. The rate of virologic breakthrough and resistance was 14.4% in the ADV+LAM group and 71.9% in the ETV group (P=0.001). Conclusions Combination of LAM and ADV therapy for up to 6 years achieved modest rates of virological suppression and resistance. ETV is not an optimal therapy because the risk of viral breakthrough to ETV increases over time.

Ze, EunYoung; Baek, Eun Kyung; Lee, Jong Jin; Chung, Han Wook; Ahn, Dae Geon; Cho, Hwan Jun; Kwon, Jae Cheol; Kim, Hyung Joon

2014-01-01

411

RICE UNIVERSITY Transport in Single Molecule Transistors  

E-print Network

RICE UNIVERSITY Transport in Single Molecule Transistors by Lam H. Yu A Thesis Submitted in Partial Houston, Texas January, 2006 #12;Abstract Transport in Single Molecule Transistors by Lam H. Yu molecule transistors (SMTs), nanometer-scale transistors in which charge transport occurs through

Natelson, Douglas

412

Taxonomic re-evaluation of the Azolla genus in Portugal A.L. PEREIRA, G. TEIXEIRA, I. SEVINATE-PINTO, T. ANTUNES and F. CARRAPIO  

E-print Network

of the genus. This genus is divided into two subgenera: Azolla, with two sections ­ Azolla (A. filiculoides Lam. filiculoides Lam., A. caroliniana Willd. and/or Azolla mexicana Presl., the latter included in the last edition the Azolla specimens, collected from several locations in Portugal, were identified as Azolla filiculoides

Carrapiço, Francisco

413

Payroll & Records Management University of Delaware  

E-print Network

Graduate student labor (not on contract) Undergraduate student labor Miscellaneous wage employees #12Payroll & Records Management University of Delaware Last Updated: 11/14/2011 Labor Allocation Module (LAM) Procedural Manual #12;#12;i Labor Allocation Module (LAM) Procedural Manual Contents Page 1

Firestone, Jeremy

414

Increasing Interoperability of E-Learning Content in Moodle within a Franco-Arabo Educative Context  

ERIC Educational Resources Information Center

This article examines how Moodle, as an open-source Learning Management System, can be made more interoperable. The authors tested two software standards, LAMS and RELOAD, compatible with socio-constructivism norms. The analysis showed that pedagogic activities created with the LAMS-IMS Learning Design Level A Format are useable with Moodle but…

El Harrassi, Souad; Labour, Michel

2010-01-01

415

Think globally, act locally: library, archive, and museum collaboration  

Microsoft Academic Search

While the collections libraries, archives, and museums (LAMs) manage remain necessarily fragmented in the real world, potential users of these collections increasingly expect to experience the world of information as accessible from a single search online. The groundwork for success in a networked environment is laid in local collaborations, such as creating cohesiveness among LAMs belonging to the same organization.

Günter Waibel; Ricky Erway

2009-01-01

416

BioMed Central Page 1 of 13  

E-print Network

Genetics and Developmental Biology, British Columbia Cancer Research Centre, Vancouver, BC, Canada, 2 Lonergan1, Raymond T Ng2, Calum MacAulay3, Wan L Lam1 and Stephen Lam3 Address: 1Department of Cancer of Cancer Imaging, British Columbia Cancer Research Centre, Vancouver, BC, Canada Email: Raj Chari* - rchari

Ng, Raymond T.

417

The effect of small gaps in California annual grassland on above-ground biomass production  

E-print Network

- mon in grasslands. In California annual grasslands, pat- ches of Lolium multi¯orum Lam. and Bromus. multi¯orum/half B. hordeaceus and two quarters L. multiflorum/two quarters B. hordeaceus (nine introduced grasses, Lolium multi- ¯orum Lam. (Italian ryegrass; all nomenclature per Hickman, 1993

Fehmi, Jeffrey S.

418

Recherche des facteurs responsables du dclenchement du tallage chez le ray-grass d'Italie (Lolium multiflorum  

E-print Network

-grass (Lolium multiflorum lam.) under field conditions. The relationship between climate and time from emergenceRecherche des facteurs responsables du déclenchement du tallage chez le ray-grass d'Italie (Lolium multiflorum lam.) en conditions naturelles Jean-Claude SIMON Richard DELECOLLE LN.R.A., Station d'Agronomie, 4

Boyer, Edmond

419

Nursing students celebrate their vocation with Blessing of the Hands  

Microsoft Academic Search

The University of Notre Dame Australia, Sydney Campus, has welcomed its new Nursing students with the annual Blessing of the Hands ceremony.Notre Dame Chaplain, Fr Lam Vu, and Assistant Chaplain, Fr John Neill, blessed the hands of each student individually. Fr Lam prayed that the future nurses would be filled with the Holy Spirit as they carried out their vocation

Elizabeth Fenech

2012-01-01

420

Be more specific! Laser-assisted microdissection of plant cells  

Microsoft Academic Search

Laser-assisted microdissection (LAM) is a powerful tool for isolating specific tissues, cell types and even organelles from sectioned biological specimen in a manner conducive to the extraction of RNA, DNA or protein. LAM, which is an established technique in many areas of biology, has now been successfully adapted for use with plant tissues. Here, we provide an overview of the

Robert C. Day; Ueli Grossniklaus; Richard C. Macknight

2005-01-01

421

Molecular Genetic Analysis of the Nested Drosophila melanogaster Lamin C Gene  

PubMed Central

Lamins are intermediate filaments that line the inner surface of the nuclear envelope, providing structural support and making contacts with chromatin. There are two types of lamins, A- and B-types, which differ in structure and expression. Drosophila possesses both lamin types, encoded by the LamC (A-type) and lamin Dm0 (B-type) genes. LamC is nested within an intron of the essential gene ttv. We demonstrate that null mutations in LamC are lethal, and expression of a wild-type LamC transgene rescues lethality of LamC but not ttv mutants. Mutations in the human A-type lamin gene lead to diseases called laminopathies. To determine if Drosophila might serve as a useful model to study lamin biology and disease mechanisms, we generated transgenic flies expressing mutant LamC proteins modeled after human disease-causing lamins. These transgenic animals display a nuclear lamin aggregation phenotype remarkably similar to that observed when human mutant A-type lamins are expressed in mammalian cells. LamC aggregates also cause disorganization of lamin Dm0, indicating interdependence of both lamin types for proper lamina assembly. Taken together, these data provide the first detailed genetic analysis of the LamC gene and support using Drosophila as a model to study the role of lamins in disease. PMID:15965247

Schulze, Sandra R.; Curio-Penny, Beatrice; Li, Yuhong; Imani, Reza A.; Rydberg, Lena; Geyer, Pamela K.; Wallrath, Lori L.

2005-01-01

422

Distribution of Spoligotyping Defined Genotypic Lineages among Drug-Resistant Mycobacterium  

E-print Network

patterns. Five of these 20 unique patterns corresponded to orphan patterns in the SITVIT2 database, while 4%; previously designated as LAM7-TUR), Beijing (6/95, 6.3%), and Latin-America & Mediterranean (LAM, 5/95 or 5

Boyer, Edmond

423

Lymphatic Endothelial Differentiation in Pulmonary Lymphangioleiomyomatosis Cells  

PubMed Central

Pulmonary lymphangioleiomyomatosis (LAM) is a rare, low-grade neoplasm affecting almost exclusively women of childbearing age. LAM belongs to the family of perivascular epithelioid cell tumors, characterized by spindle and epithelioid cells with smooth muscle and melanocytic differentiation. LAM cells infiltrate the lungs, producing multiple, bilateral lesions rich in lymphatic channels and forming cysts, leading to respiratory insufficiency. Here we used antibodies against four lymphatic endothelial markers—podoplanin (detected by D2-40), prospero homeobox 1 (PROX1), vascular endothelial growth factor receptor 3 (VEGFR-3), and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1)—to determine whether LAM cells show lymphatic differentiation. Twelve of 12 diagnostic biopsy specimens (early-stage LAM) and 19 of 19 explants (late-stage LAM) showed immunopositivity for D2-40 in most neoplastic cells. PROX1, VEGFR-3, and LYVE1 immunoreactivity varied from scarce in the early stage to abundant in the late stage. Lymphatic endothelial, smooth muscle, and melanocytic markers were partially co-localized. These findings indicate that lymphatic endothelial differentiation is a feature of LAM and provide evidence of a previously unidentified third lineage of differentiation in this neoplasm. This study has implications for the histological diagnosis of LAM, the origin of the neoplastic cells, and potential future treatment with drugs targeting lymphangiogenesis. PMID:23609227

Davis, Jennifer M.; Hyjek, Elizabeth; Husain, Aliya N.; Shen, Le; Jones, Jennifer

2013-01-01

424

SHARKS, SKATES, RAYS, AND CHIMAERAS  

E-print Network

feet 5 inches 26 feet 5 inches nd hary... ..... Carcharias taurus... . . . . . 10 feet 5 inches 12 feet 3 inches 15 feet 11 inches Porbeagle ·....... 1 LamTUl TUlSUS........... ... 10 feet 12 feet Sall10n shark. .... Lam

425

78 FR 11733 - Norfolk Southern Railway Company-Abandonment Exemption-in Calhoun County, AL  

Federal Register 2010, 2011, 2012, 2013

...milepost LAM 508.08 (near the intersection of W. 10th and Pipe Streets) and milepost LAM 509.89 (to the east of the eastern end...in favor of complainant within the two-year period; and (4) the requirements at 49 CFR 1105.7(c)...

2013-02-19

426

The civil justice system and going concern audit reports: Comments on “Auditors’ decision-making under going concern uncertainties in low litigation risk environments: Evidence from Hong Kong”  

Microsoft Academic Search

Lam and Mensah [Lam, K., Mensah, Y.M., 2006. Auditors’ decision-making under going concern uncertainties in low litigation risk environments: Evidence from Hong Kong. J. Account. Public Pol. 25 (6), this issue] provide some valuable insight on auditors’ choice of going concern audit reports in an environment where the civil justice system affords, from the standpoint of the plaintiff, fewer remedies

Randall E. LaSalle

2006-01-01

427

JOURNAL OF BACTERIOLOGY, Jan. 2007, p. 151159 Vol. 189, No. 1 0021-9193/07/$08.00 0 doi:10.1128/JB.01224-06  

E-print Network

of Pseudomonas aeruginosa Strain Pa5196 Type IV Pilins with Mycobacterium-Like -1,5-Linked D and lipoarabi- nomannan (LAM) polymers of mycobacteria, including Mycobacterium tuberculosis and Mycobacterium leprae. An- tibodies raised against M. tuberculosis LAM specifically identified the glycosylated pilins

Burrows, Lori

428

Lymphangioleiomyomatosis: differential diagnosis and optimal management  

PubMed Central

Lymphangioleiomyomatosis (LAM) is an uncommon disease presented as diffuse thin-walled cystic changes in the lung. The main differential diagnoses include pulmonary Langerhans’ histiocytosis (PLCH), Birt-Hogg-Dubé syndrome (BHD), lymphoid interstitial pneumonia (LIP), and amyloidosis. A combination of clinical, radiological, and pathological approaches as well as genetic testing will clarify the diagnosis in most cases. LAM is a disease almost exclusively in women. Dyspnea, pneumothorax, and hemoptysis are common presentations in LAM patients. LAM is also a lymphatic disorder affecting lymphatic vessels and lymph nodes. Chylothorax, chylous ascites, and lymphangiomyomas are frequently seen. LAM can present sporadically as a single entity or as part of tuberous sclerosis complex (TSC). Angiomyolipoma (AML) is a characteristic extra-pulmonary lesion, either found in association with sporadic or TSC-related LAM. High-risk populations should be screened for LAM, including adult women with TSC and female patients with spontaneous pneumothorax, AMLs in the kidney, and diffuse cystic lung diseases. Definitive diagnosis of LAM is based on a high level of clinical suspicion on presentation supported by pathological findings or by a distinct feature, such as a history of TSC, AMLs in the kidney, chylothorax, or chylous ascites. Vascular endothelial growth factor-D (VEGF-D) in serum is a noninvasive and reliable diagnostic biomarker. In experienced centers, trans-bronchial lung biopsy (TBLB) provides a convenient and safe way to obtain lung specimens for diagnostic purposes. An effective treatment for LAM is now available, namely using a mechanistic target of rapamycin (mTOR) inhibitor such as sirolimus. Efficacy of sirolimus has been confirmed in clinical trials. Research in other molecular-targeted therapies is under investigation. A previously little-known rare disease with no cure is now better understood with regards to its pathogenesis, diagnosis, and management. In this review, current knowledge in diagnosis and differential diagnosis of LAM will be discussed, followed by the discussion of therapy with mTOR inhibitors. PMID:25187723

Xu, Kai-Feng; Lo, Bee Hong

2014-01-01

429

In pulmonary lymphangioleiomyomatosis expression of progesterone receptor is frequently higher than that of estrogen receptor.  

PubMed

Lymphangioleiomyomatosis (LAM) of the lung is a rare low-grade malignancy affecting primarily women of childbearing age. LAM is characterized by the proliferation of SMA and HMB-45 positive spindle-shaped and epithelioid cells throughout the lung in the form of discrete lesions causing cystic destruction and ultimately respiratory insufficiency. LAM occurs sporadically or in patients with tuberous sclerosis complex (TSC) and is etiologically linked to mutations in the TSC1 and TSC2 genes. Although LAM cells are known to express estrogen and progesterone receptors (ER and PR, respectively), their respective expression level was never determined. Therefore, here we measured the immunohistochemical expression of ERs and PRs in a large series of pulmonary LAM cases using the Aperio Spectrum Analysis Platform. Our case series comprised open lung biopsy specimens from 20 LAM patients and lungs explanted during the course of lung transplant from 24 patients. All cases were positive for ER and PR. PR expression was statistically significantly higher than ER in 80 % of the biopsies while ER predominated only in one case. Specimens from explanted cases of LAM had relatively fewer PR-positive nuclei. As a result, PR expression was significantly higher than ER in 38 % of the cases, whereas ER predominated in 33 %. Overall, PR expression predominated in 57 % of cases and ER in 21 %. These data indicate that PR frequently prevails over ER in pulmonary LAM. LAM is unusual in its high PR/ER ratio; other female neoplasms show a definite prevalence of ER. Our findings therefore warrant further study of PR function in LAM. PMID:24570392

Gao, Ling; Yue, Michael M; Davis, Jennifer; Hyjek, Elisabeth; Schuger, Lucia

2014-04-01

430

Fruit mineral contents of six wild species of the North Andean Patagonia, Argentina.  

PubMed

The fruit mineral contents (K, Ca, Ba, Br, Zn, Co, Cr, Fe, Na, Rb, Cs, and Sr) of four native and two exotic naturalized shrubs growing in different areas of the Andean Patagonian region of Argentina were investigated. Native species Berberis darwinii, Berberis microphylla (Berberidaceae), Aristotelia chilensis (Elaeocarpaceae) and Ribes magellanicum (Saxifragaceae) produce small berries while the fruits of the exotic species Rosa rubiginosa and Rosa canina (Rosaceae) are aggregates of aquenes. They are used to prepare jams, tea, liquors, and ice creams. Native shrub fruits had higher content of Br, Zn, Co, Cr, Fe, Mo, and Na than those of the exotic naturalized species. Rosa species showed the highest contents of Ca and Ba in their fruits (the mean content doubled those of the native plant fruits). The fruit nutrient content found in the studied species was similar or higher than other values reported for fruits of temperate and tropical species in the world. PMID:18512032

Damascos, María A; Arribere, Maria; Svriz, Maya; Bran, Donaldo

2008-10-01

431

Nitrogen Limits an Invasive Perennial Shrub in Forest Understory  

Microsoft Academic Search

Plant invasions can harm communities by domination of one or more vegetation layers. We studied whether Japanese barberry\\u000a (Berberis thunbergii DC.) is limited by soil acidity or nitrogen availability in its domination of relatively undisturbed forest understories.\\u000a In two sites, one more acid than the other, we applied lime, urea, or a sawdust–sugar mix to replicate plots in established\\u000a barberry

Timothy M. Cassidy; James H. Fownes; Robin A. Harrington

2004-01-01

432

PHARMACOKINETIC STUDY OF BERBERINE FROM RASONT AND IMPLICATION OF LYSERGOL FOR ITS BIOAVAILABILITY ENHANCEMENT  

Microsoft Academic Search

The oral bioavailability of berberine from Rasont (aqueous extract of Berberis aristata DC., Family: Berberidaceae) was investigated in rats. The in vivo bioavailability enhancement potential of lysergol was also evaluated for berberine from Rasont. One animal group was dosed with freshly prepared lyophilized Rasont (Dose: 1.07 g\\/kg bodyweight equivalent to 200 mg of berberine) and the other with both freshly prepared lyophilized

Shrikant Patil; Ranjeet Prasad Dash; Sheetal Anandjiwala; Manish Nivsarkar

2012-01-01

433

Nouveautés dans les phytomédications dermatologiques du psoriasis avec application clinique  

Microsoft Academic Search

Résumé: L’auteur évoque la phytothérapie appliquée à la dermatologie. Après une présentation des expérimentations allemandes sur l’extrait de mahonia ( Berberis aquifolium (Nutt.) Pursh.) dans le psoriasis, il signale une autre plante, Rhagadiolus stellatus, qui pourrait avoir des vertus analogues. Plante ancienne de la phytothérapie, l’hamamélis fait encore l’objet de recherches et de découvertes intéressantes à considérer par le clinicien.

P. Goetz

2004-01-01

434

Lymphatics in lymphangioleiomyomatosis and idiopathic pulmonary fibrosis  

PubMed Central

The primary function of the lymphatic system is absorbing and transporting macromolecules and immune cells to the general circulation, thereby regulating fluid, nutrient absorption and immune cell trafficking. Lymphangiogenesis plays an important role in tissue inflammation and tumour cell dissemination. Lymphatic involvement is seen in lymphangioleiomyomatosis (LAM) and idiopathic pulmonary fibrosis (IPF). LAM, a disease primarily affecting females, involves the lung (cystic destruction), kidney (angiomyolipoma) and axial lymphatics (adenopathy and lymphangioleiomyoma). LAM occurs sporadically or in association with tuberous sclerosis complex (TSC). Cystic lung destruction results from proliferation of LAM cells, which are abnormal smooth muscle-like cells with mutations in the TSC1 or TSC2 gene. Lymphatic abnormalities arise from infiltration of LAM cells into the lymphatic wall, leading to damage or obstruction of lymphatic vessels. Benign appearing LAM cells possess metastatic properties and are found in the blood and other body fluids. IPF is a progressive lung disease resulting from fibroblast proliferation and collagen deposition. Lymphangiogenesis is associated with pulmonary destruction and disease severity. A macrophage subset isolated from IPF bronchoalveolar lavage fluid (BALF) express lymphatic endothelial cell markers in vitro, in contrast to the same macrophage subset from normal BALF. Herein, we review lymphatic involvement in LAM and IPF. PMID:22941884

Glasgow, Connie G.; El-Chemaly, Souheil; Moss, Joel

2013-01-01

435

Dectin-2 is a direct receptor for mannose-capped lipoarabinomannan of mycobacteria.  

PubMed

Mycobacteria possess various immunomodulatory molecules on the cell wall. Mannose-capped lipoarabinomannan (Man-LAM), a major lipoglycan of Mycobacterium tuberculosis, has long been known to have both inhibitory and stimulatory effects on host immunity. However, the direct Man-LAM receptor that explains its pleiotropic activities has not been clearly identified. Here, we report that a C-type lectin receptor Dectin-2 (gene symbol Clec4n) is a direct receptor for Man-LAM. Man-LAM activated bone-marrow-derived dendritic cells (BMDCs) to produce pro- and anti-inflammatory cytokines, whereas it was completely abrogated in Clec4n(-/-) BMDCs. Man-LAM promoted antigen-specific T cell responses through Dectin-2 on DCs. Furthermore, Man-LAM induced experimental autoimmune encephalitis (EAE) as an adjuvant in mice, whereas Clec4n(-/-) mice were resistant. Upon mycobacterial infection, Clec4n(-/-) mice showed augmented lung pathology. These results demonstrate that Dectin-2 contributes to host immunity against mycobacterial infection through the recognition of Man-LAM. PMID:25176311

Yonekawa, Akiko; Saijo, Shinobu; Hoshino, Yoshihiko; Miyake, Yasunobu; Ishikawa, Eri; Suzukawa, Maho; Inoue, Hiromasa; Tanaka, Masato; Yoneyama, Mitsutoshi; Oh-Hora, Masatsugu; Akashi, Koichi; Yamasaki, Sho

2014-09-18

436

Linear atrophoderma of Moulin: a distinct entity?  

PubMed

Linear atrophoderma of Moulin (LAM) is a rare dermatologic disorder characterized by a hyperpigmented atrophoderma that consistently follows the lines of Blaschko. There are many clinical and histologic similarities between LAM, atrophoderma of Pasini and Pierini (APP), and morphea, and whether LAM represents part of a disease spectrum or its own distinct entity is debated. This case of a 16-year-old boy with LAM supports the hypothesis that LAM, APP, and morphea are a spectrum of disorders rather than unique entities. Although the patient's overall clinical picture supports a diagnosis of LAM with hyperpigmented, depressed lesions following the lines of Blaschko and perivascular lymphocytic infiltrate on biopsy, the bilateral presentation typical of APP, collagen entrapment of eccrine ducts typical of morphea, and changes in dermal collagen illustrate features spanning all three disorders, suggesting a relationship between these conditions that represents a spectrum of disease. Furthermore, a review of all reported cases of LAM in the literature suggests an evolving definition beyond what Moulin and colleagues originally described, including features related to those of APP and morphea. PMID:23046463

de Golian, Emily; Echols, Kathryn; Pearl, Henna; Davis, Loretta

2014-01-01

437

Efficacy of entecavir-tenofovir combination therapy for chronic hepatitis B patients with multidrug-resistant strains.  

PubMed

The emergence of multidrug-resistant (MDR) strains of hepatitis B virus (HBV) is a major concern. This study aimed to investigate the efficacy and safety of combination therapy with entecavir (ETV) plus tenofovir disoproxil fumarate (TDF) against MDR HBV. To adjust for differences in baseline characteristics, inverse probability weighting (IPW) using propensity scores for the entire cohort and weighted Cox proportional hazards models were applied. Ninety-three consecutive patients who were treated with ETV-TDF combination therapy for >6 months were included; at baseline, 45 were infected with HBV strains with genotypic resistance to lamivudine (LAM) and ETV (the LAM/ETV-R group), 28 with strains resistant to LAM and adefovir (ADV) (the LAM/ADV-R group), and 20 with strains resistant to LAM, ETV, and ADV (the LAM/ETV/ADV-R group). The median duration of rescue therapy was 13.0 (range, 6.7 to 31.7) months. Seventy-four of 93 patients (79.6%) achieved complete virologic suppression, after a median of 4.5 (95% confidence interval, 3.0 to 6.0) months. The cumulative probability of complete virologic suppression at month 6 was 63.6% (55.7%, 75.0%, and 65.0% in the LAM/ETV-R, LAM/ADV-R, and LAM/ETV/ADV-R groups, respectively). During the treatment period, these probabilities were not significantly different across the resistance profiles before and after IPW (P = 0.072 and P = 0.510, respectively). In multivariate analysis, a lower baseline HBV DNA level, but not resistance profiles, was an independent predictor of complete virologic suppression. Renal dysfunction was not observed during the treatment period. In conclusion, rescue therapy with ETV-TDF combination is efficient and safe in patients infected with MDR HBV strains regardless of the antiviral drug resistance profiles. PMID:25155601

Lee, Yun Bin; Lee, Jeong-Hoon; Lee, Dong Hyeon; Cho, Hyeki; Ahn, Hongkeun; Choi, Won-Mook; Cho, Young Youn; Lee, Minjong; Yoo, Jeong-Ju; Cho, Yuri; Cho, Eun Ju; Yu, Su Jong; Kim, Yoon Jun; Yoon, Jung-Hwan; Kim, Chung Yong; Lee, Hyo-Suk

2014-11-01

438

Application of sensitive and specific molecular methods to uncover global dissemination of the major RDRio Sublineage of the Latin American-Mediterranean Mycobacterium tuberculosis spoligotype family.  

PubMed

The Latin American-Mediterranean (LAM) family of Mycobacterium tuberculosis is believed to be the cause of approximately 15% of tuberculosis cases worldwide. Previously, we defined a prevalent sublineage of the LAM family in Brazil by a single characteristic genomic deletion designated RD(Rio). Using the Brazilian strains, we pinpoint an Ag85C(103) single nucleotide polymorphism (SNP) (screened by restriction fragment length polymorphism [RFLP] analysis) that correctly identified all LAM family strains. Importantly, all RD(Rio) strains concomitantly possessed the RD174 deletion. These genetic signatures, along with a newly developed multiplex PCR for rapid differentiation between "wild-type" and RD(Rio) strains, were then used to analyze an international collection of M. tuberculosis strains. RD(Rio) M. tuberculosis was identified from four continents involving 11 countries. Phylogenetic analysis of the IS6110-RFLP patterns from representative RD(Rio) and LAM strains from Brazil, along with all representative clusters from a South African database, confirmed their genetic relatedness and transcontinental transmission. The Ag85C(103) SNP RFLP, as compared to results obtained using a PCR method targeting a LAM-restricted IS6110 element, correctly identified 99.8% of LAM spoligotype strains. Together, these tests were more accurate than spoligotyping at categorizing strains with indefinable spoligotypes and segregated true LAM strains from those with convergent spoligotypes. The fact that RD(Rio) strains were identified worldwide highlights the importance of this LAM family sublineage and suggests that this strain is a global threat that should be specifically targeted by public health resources. Our provision of simple and robust molecular methods will assist the evaluation of the LAM family and the RD(Rio) sublineage. PMID:18234868

Gibson, Andrea L; Huard, Richard C; Gey van Pittius, Nicolaas C; Lazzarini, Luiz Claudio Oliveira; Driscoll, Jeffrey; Kurepina, Natalia; Zozio, Thierry; Sola, Christophe; Spindola, Silvana Miranda; Kritski, Afrânio L; Fitzgerald, Daniel; Kremer, Kristin; Mardassi, Helmi; Chitale, Poonam; Brinkworth, Jessica; Garcia de Viedma, Dario; Gicquel, Brigitte; Pape, Jean W; van Soolingen, Dick; Kreiswirth, Barry N; Warren, Robin M; van Helden, Paul D; Rastogi, Nalin; Suffys, Philip N; Lapa e Silva, Jose; Ho, John L

2008-04-01

439

Plants used for the treatment of diabetes in Israel.  

PubMed

In an extensive ethnobotanical survey (130 informants) of the medicinal plants of Israel, 16 species were found to be used for hypoglycaemic treatments. The list includes Achillea fragrantissima (Forssk.) Sch.-Bip, Ammi visnaga (L.) Lam, Atriplex halimus L., Capparis spinosa L., Ceratonia siliqua L., Cleome droserifolia (Forssk.) Del., Eryngium creticum Lam., Inula viscosa (L.) Ait., Matricaria aurea (Loefl.) Sch.-Bip, Origanum syriaca L., Paronychia argentea Lam, Prosopis farcta (Banks et Sol.) Macbride, Salvia fruticosa Mill., Sarcopoterium spinosum (L.) Sp., and Teucrium polium L.; eight of them (marked with an asterisk) are first recorded here as used for this purpose. PMID:3613607

Yaniv, Z; Dafni, A; Friedman, J; Palevitch, D

1987-01-01

440

Surgical treatment of chylothorax caused by lymphangioleiomyomatosis  

PubMed Central

Lymphangioleiomyomatosis (LAM) is a rare progressive disease caused by infiltration of smooth muscle-like cells in lymph vessels as well as the lung. We report a case of pulmonary LAM in a 22-year-old female with shortness of breath, recurrent pneumothorax and chylous pleural effusions. Multiple ligation of thoracic in lower part of thoracic duct was performed and biopsy of thoracic duct confirmed the diagnosis of LAM. The operation was successful and the patient was discharged. Although the thoracic duct involvement is extensive, multiple ligation in lower part of thoracic duct may be a good choice. PMID:24605238

Liu, Mingliang; Wu, Bingqun; Chang, Dong; Zhang, Shuhong; Gong, Min

2014-01-01

441

Mycobacterium tuberculosis Latin American-Mediterranean family and its sublineages in the light of robust evolutionary markers.  

PubMed

Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of the LAM family and its major sublineages circulating in Russia and neighboring countries. A total of 250 M. tuberculosis isolates were confirmed to belong to the LAM family based on the analysis of the LAM-specific single-nucleotide polymorphisms (SNPs) in the Rv3062 and Rv0129c genes. At this stage, the family status was rectified for 121 isolates misleadingly assigned by CRISPR spoligotyping to non-LAM families (T1- or T5-RUS1). Consequently, the reestimated LAM prevalence rate increased 2-fold in Russia and Kazakhstan and 4-fold in Belarus. The majority (91.8 to 98.7%) of the LAM isolates from all three countries belonged to the LAM-RUS sublineage. In contrast, the Ibero-American LAM RD-Rio sublineage was identified in only 7 Russian isolates. Taken together, our findings and further analyses suggest a monophyletic origin of LAM-RUS: at a historically distant time, in Russia, in a small founding bacterial/human population. Its dissemination pattern and high prevalence rate in Northern Eurasia may indicate a long-term coexistence of the LAM-RUS sublineage and local human populations hypothetically leading to coadaptation and reduced pathogenicity of the relatively more ancient clones, such as spoligotype international type 254 (SIT254), compared to the more recent SIT252 and SIT266 clones. In contrast, rare LAM RD-Rio isolates were likely brought to Russia through occasional human contact. The spread of RD-Rio strains is not as global as commonly claimed and is determined largely by human migration flows (rather than by pathobiological properties of these strains). Consequently, a host population factor appears to play a major role in shaping the in situ dissemination pattern of the imported strains in an autochthonous population. PMID:24584500

Mokrousov, Igor; Vyazovaya, Anna; Narvskaya, Olga

2014-05-01

442

Recovery of chylous pulmonary congestion in tuberous sclerosis complex-associated lymphangioleiomyomatosis.  

PubMed

Chyloptysis and chylous pulmonary congestion are extremely rare complications of lymphangioleiomyomatosis (LAM). We report a case of a 50-year-old woman with tuberous sclerosis complex-associated LAM, who presented with expectorating milky-white bronchial casts. She was diagnosed with chyloptysis and chylous pulmonary congestion by sputum analysis. Her symptoms and lung infiltration were improved by oral sirolimus therapy; moreover, serum Krebs von den Lungen-6 (KL-6) levels paralleled the symptoms and lung infiltration of these complications. We suggest that serum KL-6 may be a useful monitoring biomarker of chyloptysis and chylous pulmonary congestion in LAM. PMID:25326562

Kinoshita, Yoshiaki; Sakamoto, Atsuhiko; Hidaka, Kouko

2014-01-01

443

Radar observations and physical modeling of binary near-Earth asteroid (1862) ApolloT.F. Ford, L.A.M. Benner, M. Brozovic, C.B. Ledford, M.C. Nolan, J.D. Giorgini, S.J. Ostro, J.L. Margot.  

NASA Astrophysics Data System (ADS)

Binary asteroid 1862 Apollo has an extensive observational history allowing many of its characteristics to be investigated. Apollo was one of the first objects to show evidence for the YORP effect (Kaasalainen et al. 2007, Nature 446, 420) and its mass has been estimated by detection of the Yarkovsky effect (Nugent et al. 2012, AJ 144, 60; Farnocchia et al. 2013, Icarus 224, 1). We observed Apollo at Arecibo and Goldstone from Oct. 29-Nov. 13, 2005, obtaining a series of echo power spectra and delay-Doppler images that achieved resolutions as high as 7.5 m/pixel. The Arecibo images show that Apollo is a binary system with a rounded primary that has two large protrusions about 120 deg apart in longitude. We used the Arecibo data and published lightcurves to estimate the primary's 3D shape. Our best fit has major axes of ~1.8x1.5x1.3 km and a volume of ~1.6 km^3. The protrusions have lengths of ~300 and 200 m, are on the primary's equator, and give Apollo a distinctly different appearance from the primaries with equatorial ridges seen with other binary near-Earth asteroids. We estimated the pole by starting with the Kaasalainen et al. spin vector of ecliptic (longitude, latitude)=(50 deg, -71 deg) +- 7 deg and letting it float. Our best fit has a pole within 11 deg of (longitude, latitude)=(71, -72). Convex models produced from inversion of lightcurves by Kaasalainen et al. and thermal infrared data by Rozitis et al. (2013, A&A 555, A20) are more oblate than our model, do not show protrusions, and have somewhat different pole directions. The Arecibo images reveal weak but persistent echoes from a satellite on Nov. 1 and 2 but cover only a fraction of its orbit. The images are insufficient to estimate the satellite's shape and yield a rough estimate for its long axis of 190 m. Preliminary fits give an orbital period of ~27.0-27.5 h and a semimajor axis of ~3.5-4.0 km, implying a mass of 2.8-3.9E12 kg and a bulk density of 1.7-2.4 g/cm^3. The density is consistent with estimates reported by Rozitis et al. and Farnocchia et al., providing the first independent test using a binary to estimate the density of near-Earth asteroid that has also been estimated through detection of the Yarkovsky effect.

Ford, Thomas; Benner, Lance A.; Brozovic, Marina; Leford, Bruce; Nolan, Michael C.; Giorgini, Jon D.; Ostro, Steve J.; Margot, Jean-Luc

2014-11-01

444

The Internet as a Glocal Discourse Environment: A Commentary on "Second Language Socialization in a Bilingual Chat Room" by Wan Shun Eva Lam and "Second Language Cyberhetoric: A Study of Chinese L2 Writers in an Online Usenet Group" by Joel Bloch  

ERIC Educational Resources Information Center

The Internet as a worldwide literacy practice environment has created a new situation in communication, providing a new dynamic field for research. On the basis of the two articles under discussion, this commentary develops three main aspects of the Internet: as an informal learning environment for English as a second/foreign language; as a…

Koutsogiannis, Dimitris; Mitsikopoulou, Bessie

2004-01-01

445

Terahertz imaging and quantum cascade laser based devices  

E-print Network

The terahertz (THz) frequency range (f=0.3-10 THz, [lambda]=30-1000 lam) is much less technologically developed that the adjacent microwave and infrared frequency ranges, but offers several advantages for imaging applications: ...

Lee, Alan Wei Min

2010-01-01

446

LYNN -----SEMINARS / CONFERENCES / BUILDING SPECIALIST Seminar Speakers and Research Visitors : i.e. lodging, shuttles, & misc. travel reimbursements.  

E-print Network

rebudgeting Labor Allocation Module (LAM) for workload & grant assignments Effort certifications Faculty / building / telephone problems, etc. Faculty Searches New Hires: workstudy students / tutorial lab SPECIALIST Preparing faculty reimbursements (faculty travel) Allocation/sign off on all faculty receipts

Cakoni, Fioralba

447

A new approach to resolving climate-cryosphere relations: Downscaling climate dynamics to glacier-scale mass and energy balance without statistical scale linking  

Microsoft Academic Search

Novel combination of multiscale modeling systemsProcess-resolving mass balance model is successfully driven by direct LAM outputHigh-resolution atmospheric modeling should be pursued in cryospheric research

Thomas Mölg; Georg Kaser

2011-01-01

448

Himalayan Drama  

E-print Network

famous work Bodhipatha Pradipa (Byam-chub-Lam-sgron). Tanjur contents also include poetic alamkara like Kavyadarsha of Dandin. Chhanda ratnakara of Ratnakarashanti (rhetoric) Kalidas's Meghaduta, Panini. Kalapa and Sarasvata grammars, Ayurveda...

Ghosh, Bhajagovinda

1997-01-01

449

Evapotranspiration and Leachate Quality of Warm-season Turf and Native Grasses under Different Texas Landscape Climates  

E-print Network

.) Kutze.] alone, Red Oak [Quercus shumardii Buckl.] alone, St. Augustinegrass plus Red Oak, native grasses [Muhlenbergia capillaries (Lam.) Trin. and Schizachyrium scoparium (Michx.) Nash], and native grasses plus Red Oak in College Station (CS) and San...

Pannkuk, Timothy Richard

2012-02-14

450

2009 by the Regents of the University of Minnesota. All rights reserved. The University of Minnesota is an equal opportunity educator and employer. To request this information in alternative formats or for disability accommodations, contact the Office of  

E-print Network

data, voice, and video communications, including the Univer- sity's copper and fiber cable plant fiberconnectivity and collocation Lam bdaRAIL W i-Fi Jointpow ers agreem ent Data C enterconsolidation W eb

Minnesota, University of

451

Under consideration for publication in J. Fluid Mech. 1 Absolute linear instability in laminar and  

E-print Network

Science, Xi'an Jiaotong-Liverpool University, 111 Ren Ai Road, Dushu Lake Higher Education Town, Suzhou. For a lam- inar base state, a stratification in dynamic viscosity produces instability; for a turbulent base

Paris-Sud XI, Université de

452

Forage, soil and water quality responses to animal waste application  

E-print Network

applications to bermudagrass (Cynodon dactylon) and ryegrass (Lolium multiflorum Lam) sods were used to quantify N03 leaching below the root zone of a Darco loamy fine sand (loamy, siliceous, thermic Grossarenic Paleudult) located at Overton, Texas. Rates of 0...

Johnson, Andrew Floyd

2012-06-07

453

Chemopreventive and anti-cancer properties of the aqueous extract of flowers of Butea monosperma  

Microsoft Academic Search

Ethnopharmacological relevanceButea monosperma (Lam.) (Fabaceae) popularly known as ‘flame of the forest’ has been widely used in the traditional Indian medical system of ‘Ayurveda’ for the treatment of a variety of ailments including liver disorders.

Tenzin Choedon; Surendra Kumar Shukla; Vijay Kumar

2010-01-01

454

The foxtail (Setaria) species-group  

Microsoft Academic Search

5 yellow foxtail, Setaria pumila (Poir) Roem. & Schult. 5 Setaria glauca (L.) Beauv. SETLU; giant foxtail, Setaria faberi Herrm. SETFA; bristly foxtail, Setaria verticillata (L.) Beauv. SETVE; knotroot foxtail, Setaria geniculata (Lam.) Beauv. SETGE.

Jack Dekker

2003-01-01

455

Movement of Sea Lamprey in the Lake Champlain Basin Eric A. Howe1, J. Ellen Marsden1,*, and Wayne Bouffard2  

E-print Network

degradation, and parasitism by sea lam- prey ultimately caused the extirpation of lake trout (Salvelinus namaycush) in Lakes Ontario, Erie, Huron, and Michigan; remnant populations sur- vived in Lake Superior

Marsden, Ellen

456

Kalyan C. Vinnakota Research Scientist I  

E-print Network

mitochondria utilizing physiological substrates cannot explain respiratory control in vivo. The Journal: study of citrate synthase. PLoS ONE 3: e1825, 2008. 11. Karpushev AV, Levchenko V, Pavlov TS, Lam V

457

Monitoring airway mucus flow and ciliary activity with optical coherence tomography  

E-print Network

. M. McWilliams, J. R. Mayo, and S. Lam, "Airway wall thickness assessed using computed tomography in chronic obstructive pulmonary disease," Chest 103(5), 1390­1396 (1993). 4. M. R. Knowles and R. C. Boucher

Oldenburg, Amy

458

Fire-scar formation and compartmentalization in Kevin T. Smith and Elaine Kennedy Sutherland  

E-print Network

Fire-scar formation and compartmentalization in oak Kevin T. Smith and Elaine Kennedy Sutherland in April 1995 and 1997 scarred Quercus prinus L. and Q. velutina Lam. Low-intensity fires scorched bark

459

Cornell University, Office of Sponsored Programs Awards Received in June 2003  

E-print Network

) NEW SOURCES OF WILD APPLE GERMPLASM AND ENHANCEMENT GERMPLASM EVALUATED FOR DISEASE RESISTANCE $15/DIVERGENCE IN DROSOPHILA PATHOGEN DEFENSES: $41,608 LAM 42976 CLARK, ANDREW G. MOLECULAR BIO-E RUNYON POSTDOC:PATRICIA

Danforth, Bryan Nicholas

460

Effect of Ethylene on Peroxidase Activity.  

National Technical Information Service (NTIS)

Ethylene increased the peroxidase activity of nine out of ten varieties of sweet potato (Ipomoea batatas (L.) Lam.) root disks tested. The increase which was observed four hours after ethylene treatment was partially overcome by carbon dioxide. The increa...

H. E. Gahagan, R. E. Holm, F. B. Abeles

1968-01-01

461

V O L U M E 4 7 1 N U M B E R 16 1 O C T O B E R \\ 8 ,2 0 0 1 I N S I D E  

E-print Network

Li (left) steadies student Elizabeth Bennett on the uneven bars at a UBC gymnastics class held at Osborne to non-competitive gymnastics.For more information call 604-822-0207. Richard Lam photo Scholar

Farrell, Anthony P.

462

Genetics Home Reference: Lymphangioleiomyomatosis  

MedlinePLUS

... in certain cells during a woman's lifetime (a somatic mutation), she may develop LAM. These women typically ... obstructive ; oxygen ; pneumothorax ; pulmonary ; respiratory ; sclerosis ; sex hormone ; somatic mutation ; spontaneous ; sporadic ; syndrome ; tissue ; tumor You may ...

463

Ann. For. Sci. 67 (2010) 603 Available online at: c INRA, EDP Sciences, 2010 www.afs-journal.org  

E-print Network

Trab. and A. tazaotana Villar in northern Morocco and A. alba Mill. in the Pyrenees. The species with other tree species such as Quercus faginea Lam., Q. ilex L., Pinus * Corresponding author: ge2macum

Boyer, Edmond

464

The Department of Mechanical and Aerospace Engineering is seeking qualified contract instructors for the following courses  

E-print Network

continuum mechanics, theory of elasticity, stress function approach, Lamé and Mitchell problems, stressKenzie Bldg., 1125 Colonel By Drive, Ottawa, Ontario, Canada, K1S 5B6, Telephone: (613) 5202600 ext 1174, Fax

Dawson, Jeff W.

465

In: Biology, Evolution and Conservation of River Dolphins... ISBN 978-60876-633-8 Editors: Manuel Ruiz-Garcia and Joseph Shostell 2010 Nova Science Publishers, Inc.  

E-print Network

as in the Amazon River basin (Figure 1). Marine Sotalia are found from Honduras to the state of Santa Catarina and Colombia) and "lam" (Nicaragua). The distribution of riverine Sotalia comprises most of the Amazon River

Solé-Cava, Antonio M.

466

Kwok Yee Michael Wong Academic qualifications  

E-print Network

Wong, "Optimal Location of Sources in Transportation Networks", J. Stat. Mech., P04017 (38 pages) (2010 Field Dynamics with Short-Term Synaptic Depression" (MPhil 2013) Yat Hong Lam, "Network Trading Model and

Wong, Michael K Y

467

Proceedings of the Sudden Oak Death Fifth Science Symposium Susceptibility of Larch, Hemlock, Sitka Spruce, and  

E-print Network

on Japanese larch (Larix kaempferi (Lam.) Carrière) in the United Kingdom (Forestry Commission 2012, Webber et. laricina (Du Roi) K. Koch), and western larch (L. occidentalis Nutt.); western and eastern hemlock (T

Standiford, Richard B.

468

75 FR 62462 - Additions to the List of Validated End-Users in the People's Republic of China: Hynix...  

Federal Register 2010, 2011, 2012, 2013

...Validated End-Users in the People's Republic of China: Hynix Semiconductor China Ltd., Hynix Semiconductor (Wuxi) Ltd. and Lam...to add three end-users, Hynix Semiconductor (China) Ltd., Hynix Semiconductor (Wuxi) Ltd....

2010-10-12