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1

Berberis buxifolia fruiting: Kinetic growth behavior and evolution of chemical properties during the fruiting period and different growing seasons  

Microsoft Academic Search

This work studied the growth of Berberis buxifolia fruits and some of their chemical attributes during the fruiting period and different growing seasons (2004\\/2005, 2005\\/2006, 2006\\/2007) from November (14 days from full flower phase) till March (126 days from full flower phase) for plants growing in a natural environment near Ushuaia city (Tierra del Fuego, Argentina). B. buxifolia fruit growth

Miriam E. Arena; Néstor Curvetto

2008-01-01

2

LAM Foundation  

MedlinePLUS

... Foundation is pleased to offer LAM patients an electronic copy of Personal Journeys With LAM, a collection ... these personal stories of courage and determination. An electronic copy was emailed to all LAM patients who ...

3

The berberis story: Berberis vulgaris in therapeutics.  

PubMed

Barberry has played a prominent role in herbal healing for more than 2,500 years. Berberis vulgaris is a common garden bush, native to Europe and the British Isles, naturalized in North America, seems to have history as old as human race. Anthropologists believe in a ritual practice or sacred object, especially by Native Americans that it works as a supernatural power or as preventive or remedy of illness. It is a deciduous shrub having yellow flowers and scarlet colored fruit in the form of berries. Twenty two alkaloids have been reported so far from root, stem leaves and fruit of this plant, which are of medicinal importance. As a herbal remedy it has no match in serving human race since ancient times. It is the most widely used drug in Homeopathic system of medicine for kidney pain and for removal of kidney stones. In this article, we present countless blessings of nature encountered through this herb which are worthy of recording. PMID:17337435

Arayne, M Saeed; Sultana, Najma; Bahadur, Saima Sher

2007-01-01

4

Hepatotoxicity evaluation of aqueous extract from Scutia buxifolia.  

PubMed

Nowadays there is an increase in the number of people taking herbals worldwide. Scutia buxifolia is used for the treatment of hypertension, but little is known about its action on liver. Thirty-two Wistar rats were divided into four groups: control and groups treated during 30 days with 100, 200 and 400 mg of lyophilized aqueous extract of S. buxifolia (SBSB)/kg of body weight. This study was planned to explore hepatotoxic effect of SBSB, which was assessed by serum transaminases (ALT and AST). Thiobarbituric acid reactive substances (TBARS) levels were determined in liver, along with thiols content (NPSH), catalase (CAT) activity and, superoxide dismutase (SOD) enzymes. Histopathological studies of liver tissue were performed. Flavonoids and phenolics were quantified in SBSB by high performance liquid chromatography with diode array detection (HPLC/DAD). We did not observe alterations on redox status (TBARS, NPSH, CAT and, SOD) in the control and experimental groups. An increase on AST activity was only observed at 200 mg of SBSB, whereas ALT score was not affected by SBSB. Moreover, no morphological alterations were observed on the hepatocytes, matching the analysed biochemical parameters. This way, we conclude that SBSB was not toxic. PMID:23812249

de Freitas, Robson Borba; Rovani, Bruno Tomazele; Boligon, Aline Augusti; de Brum, Thiele Faccim; Piana, Mariana; da Silva Jesus, Roberta; Veloso, Carolina Fantinel; Kober, Helena; Moresco, Rafael Noal; da Costa Araldi, Isabel Cristina; de Freitas Bauermann, Liliane; Athayde, Margareth Linde

2013-01-01

5

Invasive Species Taxonomic Name: Berberis vulgaris  

E-print Network

Invasive Species Taxonomic Name: Berberis vulgaris Common Name: Common barberry Physical Description: Growth Type: Berberis vulgaris is a shrub that can grow up to 10 feet in height. Branches arch://ontariowildflowers.com/mondaygarden/article.php?id=0112 http://www.ruhr-uni-bochum.de/boga/html/Berberis_vulgaris_Foto.html http

Hayden, Nancy J.

6

Antimicrobial activity of Scutia buxifolia against the honeybee pathogen Paenibacillus larvae.  

PubMed

The honeybee disease American foulbrood (AFB) is a serious problem since its causative agent (Paenibacillus larvae) has become increasingly resistant to conventional antibiotics. One of the feasible alternative treatments being used for control of this disease are plants extracts. The aim of the present work was to evaluate the effect of crude extract and fractions of Scutia buxifolia against six Paenibacillus species, including P. larvae, and its potential use for the control of AFB. In vitro activity of S. buxifolia samples against Paenibacillus species were evaluated by the disk diffusion and microdilution methods, and the minimal inhibitory concentration (MIC) were also determined. All Paenibacillus species were sensitive to crude extract and fractions of S. buxifolia. The dichloromethane (DC) fraction showed the better MIC (1.56 mg/mL), followed by ethyl acetate (EtAc) (6.25 mg/mL), n-butanol (BuOH) (25 mg/mL) and Crude extract (CE) (50 mg/mL). Toxic effect of S. buxifolia crude extracts and fractions against bees were also evaluated by the spraying application method of the same concentrations of MICs. The samples tested showed no toxic effects for the bees after 15 days of observation. These results are first time described for this species and showed that S. buxifolia presented a important activity against Paenibacillus species and proved to be a natural alternative for the prevention/control of AFB. PMID:23220240

Boligon, Aline Augusti; Brum, Thiele Faccim de; Zadra, Marina; Piana, Mariana; Alves, Camilla Filippi Dos Santos; Fausto, Viviane Pedroso; Júnior, Valdir Dos Santos Barboza; Vaucher, Rodrigo de Almeida; Santos, Roberto Christ Vianna; Athayde, Margareth Linde

2013-02-01

7

Scutia buxifolia Reissek essential oil: in vitro antioxidant and antimicrobial activities.  

PubMed

The volatile oil from the stem bark of Scutia buxifolia (Rhamnaceae) has been obtained by hydrodistillation and analyzed by GC-MS. Twenty-one components were identified representing 99.93 % of the total oil composition, spathulenol (35.87%), ?-cubebene (17.26%), germacrene D (6.43%), linalool (5.19%), carvacrol (4.05%) were the main components of S. buxifolia essential oil. Antioxidant and antimicrobial properties of the essential oil were evaluated by free radical scavenging (DPPH) assay and micro broth dilution method, respectively. S. buxifolia essential oil presented interesting radical scavenging activity (IC50 = 15.03 ± 0.11 µg/mL). The antibacterial assay showed that S. buxifolia stem bark essential oil was moderately active against the Staphylococcus aureus and Micrococcus sp. (MIC = 500 µg/mL) and Escherichia coli (250 µg/mL). To the best of our knowledge, this is the first study on the composition, antioxidant and antimicrobial activities of essential oil from the S. buxifolia collected from Brazil. PMID:25211114

Boligon, Aline A; Piana, Mariana; Brum, Thiele F de; Froeder, Amanda L F; Belke, Bianca V; Schwanz, Thiago G; Mario, Débora N; Alves, Sydney H; Athayde, Margareth L

2014-09-01

8

Phytochemical Studies of Berberis vulgaris  

Microsoft Academic Search

This study presents a phytochemical investigation of the fruits of Berberis vulgaris Linn. The isolation and structure elucidation of four compounds are discussed. The terpenoids lupeol (1) and oleanolic acid (2) and the steroids stigmasterol (3) and stigmasterol glucoside (4) are isolated for the first time from this plant. The structure and stereochemistry at various asymmetric centers were established by

S. Saied; S. Begum

2004-01-01

9

Isoquinoline alkaloids from Berberis Vulgaris subsp. Australis  

Microsoft Academic Search

Sixteen isoquinoline alkaloids were isolated from Berberis vulgaris subsp. australis. In addition to quaternary protoberberines and bisbenzylisoquinolines, a new seco-bisbenzylisoquinoline, (-)-tejedine, is reported.

Rafael Suau; Rodrigo Rico; J. Manuel López-Romero; Francisco Nájera; Ana Cuevas

1998-01-01

10

LAM (Lymphangioleiomyomatosis)  

MedlinePLUS

... LAM develop pneumothorax (noo-mo-THOR-aks), or collapsed lung. In this condition, air leaks out of the ... lung and chest wall (the pleural space). A collapsed lung can cause pain and shortness of breath. Sometimes ...

11

Germination, growth and survival of Berberis thunbergii DC. (Berberidaceae) and Berberis thunbergii var. atropurpurea in five natural environments  

Microsoft Academic Search

Germination, growth and survival of green Berberis thunbergii and its purple ornamental form, Berberis thunbergii var. atropurpurea, were compared in five natural environments (full sun meadow, edge of woods, moist woods, dry woods, pine woods) to determine\\u000a whether these two forms have a similar ability to naturalize. Berberis thunbergii and Berberis thunbergii var. atropurpurea had similar germination rates of about

Jessica D. LubellMark; Mark H. Brand

2011-01-01

12

Effect of Scutia buxifolia Reissek in nucleotidase activities and inhibition of platelet aggregation.  

PubMed

This study aimed to determine the effect of lyophilized aqueous extracts of Scutia buxifolia on NTPDase and 5'-nucleotidase activity on platelets and lymphocytes as well as the profile of the platelet aggregation. In vitro tests were used to investigate the effect of the aqueous crude extract obtained from S. buxifolia leaves (SbL) and stem bark (SbS) on enzymatic activities and platelet aggregation. The platelets and lymphocytes were exposed to lyophilized aqueous extracts of S. buxifolia at concentrations of 1-200 ?g/mL in the presence of ATP, ADP, AMP as substrates, during the enzymatic assay, as well as the platelet aggregation. The results showed that SbS and SbL potently inhibited NTPDase and 5'-nucleotidase in platelets and lymphocytes. ADP-induced aggregation was inhibited by the SbS (50, 100, and 200 ?g/mL) and SbL (200 ?g/mL). In addition, these results suggest that S. buxifolia have compounds, such as gallic acid, chlorogenic acid, caffeic acid, quercetin, rutin, and kaempferol, which cause a decrease the NTPDase and 5'-nucleotidase activity, resulting in alterations in adenine nucleotide levels and protection against ADP-induced platelet aggregation. PMID:25149058

Boligon, Aline Augusti; Pimentel, Victor Camera; Bagatini, Margarete Dulce; Athayde, Margareth Linde

2015-01-01

13

Berberis alkaloids. XV. Structure of bargustanine  

Microsoft Academic Search

Bargustanine (I), belonging to a new type of isoquinoline alkaloids, has been isolated from the phenolic fraction of the total alkaloids from the roots ofBerberis vulgaris L. Its structure has been established by chemical and spectral methods.

A. Karimov; M. M. Yusupov; R. Shakirov

1993-01-01

14

Zur Morphologie des Gynözeums von Berberis  

Microsoft Academic Search

Zusammenfassung An Hand abnorm dimerer Gynözeen vonBerberis vulgaris wird die Frage nach einer möglichen Pseudomonomerie desBerberis-Pistills neuerlich aufgeworfen. Der hier mögliche Vergleich zwischen sicheren Einzelkarpellen mit dem normalen Pistill ergibt aber einwandfrei, daß in beiden Organen die Bündel einander vollkommen entsprechen, daß also im normalen Gynözeum keine Restbündel eventuell eingeschmolzener Karpelle vorhanden sind und daß dieses Gynözeum somit echt monomer

Walter Leinfellner

1957-01-01

15

Assessment of Antioxidant Potential, Total Phenolics and Flavonoids of Different Solvent Fractions of Monotheca Buxifolia Fruit  

PubMed Central

Objectives This study was conducted to investigate the antioxidant potential of methanol extract and its derived fractions (hexane, ethyl acetate, butanol, and aqueous) of fruits of Monotheca buxifolia (Falc.) Dc., a locally used fruit in Pakistan. Methods Dried powder of the fruit of M. buxifolia was extracted with methanol and the resultant was fractionated with solvents having escalating polarity; n-hexane, chloroform, ethyl acetate, n-butanol and the residual soluble aqueous fraction. Total phenolic and total flavonoid contents were estimated for the methanol and various fractions. These fractions were also subjected to various in vitro assays to estimate the scavenging activity for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide, hydroxyl, hydrogen peroxide and reductive ability for ferric ions and phosphomolybdate assay. Results The n-butanol, aqueous and methanol fractions possessed high amount of phenolics and flavonoids compared with other fractions, and subsequently showed a pronounced scavenging activity on DPPH, ABTS, superoxide, hydroxyl and hydrogen peroxide radicals and had a potent reductive ability on ferric ion and phosphomolybdate assay. There was a found significant correlation between total phenolic and flavonoid contents and EC50 of DPPH, superoxide, hydrogen peroxide radical and phosphomolybdate assays, whereas a nonsignificant correlation was found with the hydroxyl radical and ABTS radical assay. Conclusion M. buxifolia fruit can be used as natural antioxidant source to prevent damage associated with free radicals. PMID:24298440

Jan, Shumaila; Khan, Muhammad Rashid; Rashid, Umbreen; Bokhari, Jasia

2013-01-01

16

Cytotoxic triterpenoids from Berberis koreana.  

PubMed

A bioassay-guided fractionation and chemical investigation of the trunk of Berberis koreana led to the isolation and identification of three new triterpenoids, 2 ?,3 ?,19 ?-trihydroxy-urs-12-en-24-formyl-28-oic acid (1), 2 ?,3 ?,21 ?-trihydroxy-urs-12-en-28-oic acid ( 2), and 3 ?-acetyloxy-1-oxo-olean-12-en-28-oic acid (3), along with seven known triterpenoids (4-10). The structures of these new compounds were determined through spectral analysis, including extensive 2D-NMR data. The new compounds 1-3 showed significant cytotoxicity against the A549, SK-OV-3, SK-MEL-2, and HCT-15 human tumor cell lines with IC(50) values ranging from 7.17 to 48.73?µM. PMID:21928166

Kim, Ki Hyun; Choi, Sang Un; Lee, Kang Ro

2012-01-01

17

Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat  

Microsoft Academic Search

Antioxidant activities of the ethanolic extracts of roots, twigs and leaves of common barberry (Berberis vulgaris L.) and Croatian barberry (Berberis croatica Horvat) were studied. All the extracts were found to possess some radical-scavenging and antioxidant activities, as determined by scavenging effect on the DPPH free radical, reducing power and ?-carotene–linoleic acid model system. With the exception of the ?-carotene–linoleic

M. Zovko Kon?i?; D. Kremer; K. Karlovi?; I. Kosalec

2010-01-01

18

Phytochemistry and pharmacology of berberis species.  

PubMed

The genus Berberis (Berberidaceae) includes about 500 species worldwide, some of which are widely cultivated in the north-eastern regions of Iran. This genus consists of spiny deciduous evergreen shrubs, characterized by yellow wood and flowers. The cultivation of seedless barberry in South Khorasan goes back to two hundred years ago. Medicinal properties for all parts of these plants have been reported, including: Antimicrobial, antiemetic, antipyretic, antioxidant, anti-inflammatory, anti-arrhythmic, sedative, anti-cholinergic, cholagogic, anti-leishmaniasis, and anti-malaria. The main compounds found in various species of Berberis, are berberine and berbamine. Phytochemical analysis of various species of this genus revealed the presence of alkaloids, tannins, phenolic compounds, sterols and triterpenes. Although there are some review articles on Berberis vulgaris (as the most applied species), there is no review on the phytochemical and pharmacological activities of other well-known species of the genus Berberis. For this reason, the present review mainly focused on the diverse secondary metabolites of various species of this genus and the considerable pharmacological and biological activities together with a concise story of the botany and cultivation. PMID:24600191

Mokhber-Dezfuli, Najmeh; Saeidnia, Soodabeh; Gohari, Ahmad Reza; Kurepaz-Mahmoodabadi, Mahdieh

2014-01-01

19

Phytochemistry and Pharmacology of Berberis Species  

PubMed Central

The genus Berberis (Berberidaceae) includes about 500 species worldwide, some of which are widely cultivated in the north-eastern regions of Iran. This genus consists of spiny deciduous evergreen shrubs, characterized by yellow wood and flowers. The cultivation of seedless barberry in South Khorasan goes back to two hundred years ago. Medicinal properties for all parts of these plants have been reported, including: Antimicrobial, antiemetic, antipyretic, antioxidant, anti-inflammatory, anti-arrhythmic, sedative, anti-cholinergic, cholagogic, anti-leishmaniasis, and anti-malaria. The main compounds found in various species of Berberis, are berberine and berbamine. Phytochemical analysis of various species of this genus revealed the presence of alkaloids, tannins, phenolic compounds, sterols and triterpenes. Although there are some review articles on Berberis vulgaris (as the most applied species), there is no review on the phytochemical and pharmacological activities of other well-known species of the genus Berberis. For this reason, the present review mainly focused on the diverse secondary metabolites of various species of this genus and the considerable pharmacological and biological activities together with a concise story of the botany and cultivation. PMID:24600191

Mokhber-Dezfuli, Najmeh; Saeidnia, Soodabeh; Gohari, Ahmad Reza; Kurepaz-Mahmoodabadi, Mahdieh

2014-01-01

20

Berberis spp., Barberry, Oregon Grape Holly  

Technology Transfer Automated Retrieval System (TEKTRAN)

The Encylopedia of Fruit and Nuts is designed as a research reference source on temperate and tropical fruit and nut crops. The Berberidaceae contains many edible fruited species, predominantly in two genera: Mahonia and Berberis. The tremendous diversity within barberry makes it difficult to chara...

21

Phytochemical and antioxidant studies of Berberis lycium.  

PubMed

Six compounds have been isolated from methanolic and petroleum ether extracts of Berberis lycium (Barberry). Four out of six isolated compounds are reported for the first time from this plant. Purification of different compounds has been accomplished by conventional extraction and chromatographic techniques. The compounds have been structurally characterized by IR, Low Resolution MS, (1)H-NMR and (13)C-NMR spectroscopic techniques. All plant extracts and isolated compounds were assayed for the first time for their antioxidant activity. PMID:24191322

Sabir, Shaista; Tahir, Kalim; Rashid, Naghmana; Naz, Sidra; Masood, Bilal; Shah, Muhammad Ajmal

2013-11-01

22

LAM/MPI  

NSDL National Science Digital Library

LAM/MPI is a high quality implementation of the Message Passing Interface (MPI) Standard. LAM/MPI provides high performance on a variety of platforms, from small off-the-shelf single CPU clusters to large SMP machines with high speed networks, even in heterogenous environments. In addition to high performance, LAM provides a number of usability features key to developing large scale MPI applications.

Llamas, The

23

Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat.  

PubMed

Antioxidant activities of the ethanolic extracts of roots, twigs and leaves of common barberry (Berberis vulgaris L.) and Croatian barberry (Berberis croatica Horvat) were studied. All the extracts were found to possess some radical-scavenging and antioxidant activities, as determined by scavenging effect on the DPPH free radical, reducing power and beta-carotene-linoleic acid model system. With the exception of the beta-carotene-linoleic acid test, antioxidant activity correlated well with the content of main plant antioxidants, phenols and flavonols, which suggests an important role of these compounds in overall antioxidant activity of investigated plant organs. The antioxidant activity varied mostly in relation to the organ, while no significant statistically differences were found between B. vulgaris and B. croatica. PMID:20488218

Zovko Konci?, M; Kremer, D; Karlovi?, K; Kosalec, I

2010-01-01

24

Berberis phyllody is a phytoplasma-associated disease  

Microsoft Academic Search

Berberis thunbergii atropurpurea, also known as Red barberry, is a small ornamental shrub in the family Berberidaceae. In recent years, a phyllody symptom\\u000a has been observed frequently, spreading in the shrubs in northwestern China. A phytoplasma 16S rDNA specific fragment was\\u000a amplified by PCR from Berberis plants with the phyllody symptoms. DNA sequencing and restriction fragment length polymorphism analysis revealed

Z. N. Li; J. G. Song; C. P. Zhang; X. Q. Yu; K. K. Wu; W. J. Wu; Y. F. Wu; Y. Xiang

2010-01-01

25

A pharmacological study on Berberis vulgaris fruit extract  

Microsoft Academic Search

Berberis vulgaris fruit (barberry) is known for its antiarrhythmic and sedative effects in Iranian traditional medicine. The effects of crude aqueous extract of barberry on rat arterial blood pressure and the contractile responses of isolated rat aortic rings and mesenteric bed to phenylephrine were investigated. We also examined effect of the extract on potassium currents recorded from cells in parabrachial

Mohammad Fatehi; Tarek M. Saleh; Zahra Fatehi-Hassanabad; Khadige Farrokhfal; Mostafa Jafarzadeh; Samaneh Davodi

2005-01-01

26

Berberis vulgaris as Growth Promoter in Broiler Chickens  

Microsoft Academic Search

Berberis vulgaris (Zereshk in Persian) is a member of therapeutic plants in herbal medicine. There is evidence that its root contains components, such as berberine, berbamine, culumbamine and berberubine, with a relatively wide spectrum of antimicrobial activity. The aim of the present study was to examine if the plant root has any effect on the growth of broiler chickens. The

2006-01-01

27

D ETECTING THE INFLUENCE OF ORNAMENTAL BERBERIS THUNBERGII VAR. ATROPURPUREA IN INVASIVE POPULATIONS OF BERBERIS THUNBERGII (BERBERIDACEAE) USING AFLP 1  

Microsoft Academic Search

Japanese barberry ( Berberis thunbergii DC.) is a widespread invasive plant that remains an important landscape shrub repre- sented by ornamental, purple-leaved forms of the botanical variety atropurpurea . These forms differ greatly in appearance from feral plants, bringing into question whether they contribute to invasive populations or whether the invasions represent self-sustain- ing populations derived from the initial introduction

Jessica D. Lubell; Mark H. Brand; Jonathan M. Lehrer; Kent E. Holsinger

28

Acetylcholinesterase and Butyrylcholinesterase inhibitory activities of Berberis vulgaris  

E-print Network

Berberis vulgaris is a famous medicinal plant used in folk medicine for a variety of pathological condition. Aim of the study was to determine cholinesterase inhibitory effects of crude extracts and fractions of Berberis vulgaris. Considerable inhibition of acetylcholinesterase was observed in majority cases. The hexane fraction was the most active fraction with the IC50 being 68±0.028 µg/ml. In case of butyrylcholinesterase, crude extract of the leaves found to be the most active sample with (64%) inhibition and its IC50 value was 59±0.058 µg/ml. In case of fractions, aqueous fraction of crude bark extract showed most potent inhibitory profile (IC50: 59±0.058 µg/ml). Results indicated promising potential of B. vulgaris as source of new compounds for management of Alzheimer’s Disease.

D. Kolá?; L. Wimmerová; R. Kádek

29

Some physical properties of sun-dried Berberis fruit (Berberis crataegina).  

PubMed

The present study was conducted to evaluate some physical properties of sun dried Berberis fruit as a function of moisture content, varying from 9.59% to 27.90% (w.b.). Dried fruit length, width, thickness, geometric mean diameter and sphericity, increased nonlinearly from 7.19 to 7.53 mm; 3.42 to 4.03 mm; 2.78 to 3.02 mm; 4.05 to 4.51 mm and 0.56 to 0.62, respectively with increased moisture content. When we tested 1000-dried fruits, their mass increased linearly from 3.10 to 4.89 g, the true and bulk density increased nonlinearly from 769 to 845 kg m(-3) and 389 to 395 kg m(-3), respectively; with increased moisture content. Also, porosity values of dried fruits increased nonlinearly from 49.40% to 53.30%. The lowest static coefficient of friction was found on the steel surface. The angle of repose increased nonlinearly from 20.14° to 23.20° with the increasing in the moisture content. PMID:24426054

I??kl?, Nursel Develi; Y?lmaz, Ilkay

2014-01-01

30

Identification of Berberis spp. as alternate hosts of Puccinia striiformis f. sp. tritici in China  

Technology Transfer Automated Retrieval System (TEKTRAN)

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an important disease on wheat in China because of high virulence diversity. Since the discovery of sexual stage of P. striiformis on Berberis spp., especially B. chinesensis, our interests focused on identifying Berberis spec...

31

Using HPLC to Determination the Composition and Antioxidant Activity of Berberis Vulgaris  

Microsoft Academic Search

The Berberis vulgaris (BV) plant has been extensively used as a traditional medicine in some part of Asian region especially in Western Asia. The BV fruits is claimed also to have anti-viral activities, and as a treatment for chronic candidiasis, indigestion and parasites. The present study was conducted to evaluate the antioxidative activity, nutritional and antinutritional composition of Berberis vulgaris

Parichehr Hanachi

32

The Invasion Ecology of Japanese Barberry (Berberis thunbergii) in the New England Landscape  

Microsoft Academic Search

Japanese barberry (Berberis thunbergii) has been characterized as one of the most widely known and planted exotic shrubs in the United States. It was first introduced to the US in the late 1800s. By 1920 the planting of Japanese barberry was encouraged as an ornamental shrub replacing the common barberry (Berberis vulgaris). Japanese barberry began spreading from cultivation in suburban

John A. Silander; Debra M. Klepeis

1999-01-01

33

Putative proteaceous elements of the Lomatites -type reinterpreted as new Berberis of the European Tertiary  

Microsoft Academic Search

.  ?A characteristic xeromorphic foliage occurring in the European Tertiary was originally identified as a Proteaceae according\\u000a to gross morphology, and compared with Grevillea L. and Lomatia L. (Grevillea kymeana Unger, Lomatites aquensis Saporta). On the basis of venation patterns and phyllotaxy, these elements are now reinterpreted as extinct species of Berberis L. (Berberidaceae): Berberis andreanszkyi spec. nov. occurring in the

Z. Kva?ek; B. Erdei

2001-01-01

34

Antioxidant and cytoprotective compounds from Berberis vulgaris (barberry).  

PubMed

Activity-guided fractionation of an EtOAc-soluble partition of the MeOH extract from the root bark of Berberis vulgaris L. (barberry), using a hydroxyl radical-scavenging assay, led to the isolation and identification of three phenolic compounds of a previously known structure, N-(p-trans-coumaroyl)tyramine, cannabisin G and (+/-)-lyoniresinol. Of these, cannabisin G and (+/-)-lyoniresinol exhibited antioxidant activity in this bioassay. Furthermore, it was found that cannabisin G showed cytoprotective activity in cultured MCF-7 cells modulated by hydrogen peroxide. PMID:18389483

Tomosaka, Hideyuki; Chin, Young-Won; Salim, Angela A; Keller, William J; Chai, Heebyung; Kinghorn, A Douglas

2008-07-01

35

Cytotoxic steroids from the trunk of Berberis koreana.  

PubMed

A new steroid, itesmol 3-O-palmitate (1), along with two known steroids were isolated from the trunk of Berberis koreana. The structure of 1 was determined on the basis of spectroscopic analyses involving 2D NMR and chemical reactions. Compound 1 exhibited potential antiproliferative activity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines (respective IC(50) values of 7.41, 9.20, 4.53, and 12.91 µM). PMID:22484929

Kim, Ki Hyun; Choi, Sang Un; Kim, Chung Sub; Lee, Kang Ro

2012-01-01

36

Phyto-chemical and pharmacological applications of Berberis aristata.  

PubMed

In the recent years, the interest and research in medicinal plants have increased in a great deal. Ayurvedic medicines and formulations developed from ancient Indian herbal systems are renowned for their various important applications. Berberis aristata - an Indian medicinal plant, which belongs to the family Berberidaceae is an ayurvedic herb used since ancient times. It is also known as Indian berberi, Daruharidra, Daruhaldi, Darvi and Chitra. The plant is useful as anti-pyretic, anti-bacterial, anti-microbial, anti-hepatotoxic, anti-hyperglycaemic, anti-cancer, anti-oxidant and anti-lipidemic agent. B. aristata extracts and its formulations are also useful in the treatment of diarrhoea, haemorrhoids, gynaecological disorders, HIV-AIDS, osteoporosis, diabetes, eye and ear infections, wound healing, jaundice, skin diseases and malarial fever. This review aims to highlight the ethnobotany, pharmacognosy and pharmacological uses of B. aristata which will give insights in developing potentially new bioactives from the plant scaffolds. This review will also highlight the patenting trends, the new compositions developed using the actives from B. aristata and the different assignees involved in filing patents. PMID:22808523

Potdar, Dipti; Hirwani, R R; Dhulap, Sivakami

2012-07-01

37

Berbanine: a new isoquinoline-isoquinolone alkaloid from Berberis vulgaris (Berberidaceae).  

PubMed

A new isoquinoline-isoquinolone alkaloid was isolated from the root bark of Berberis vulgaris and named berbanine. The structure was established by spectroscopic methods (including 2D NMR, HR-EI-MS). PMID:23738447

Host'álková, Anna; Novák, Zdenek; Pour, Milan; Jirosová, Anna; Opletal, Lubomír; Kunes, Jirí; Cahliková, Lucie

2013-04-01

38

Detecting the influence of ornamental Berberis thunbergii var. atropurpurea in invasive populations of Berberis thunbergii (Berberidaceae) using AFLP1.  

PubMed

Japanese barberry (Berberis thunbergii DC.) is a widespread invasive plant that remains an important landscape shrub represented by ornamental, purple-leaved forms of the botanical variety atropurpurea. These forms differ greatly in appearance from feral plants, bringing into question whether they contribute to invasive populations or whether the invasions represent self-sustaining populations derived from the initial introduction of the species in the late 19th century. In this study we used amplified fragment length polymorphism (AFLP) markers to determine whether genetic contributions from B. t. var. atropurpurea are found within naturalized Japanese barberry populations in southern New England. Bayesian clustering of AFLP genotypes and principal coordinate analysis distinguished B. t. var. atropurpurea genotypes from 85 plants representing five invasive populations. While a single feral plant resembled B. t. var. atropurpurea phenotypically and fell within the same genetic cluster, all other naturalized plants sampled were genetically distinct from the purple-leaved genotypes. Seven plants from two different sites possessed morphology consistent with Berberis vulgaris (common barberry) or B. ×ottawensis (B. thunbergii × B. vulgaris). Genetic analysis placed these plants in two clusters separate from B. thunbergii. Although the Bayesian analysis indicated some introgression of B. t. var. atropurpurea and B. vulgaris, these genotypes have had limited influence on extant feral populations of B. thunbergii. PMID:21632395

Lubell, Jessica D; Brand, Mark H; Lehrer, Jonathan M; Holsinger, Kent E

2008-06-01

39

Berberis vulgaris root bark extract prevents hyperoxaluria induced urolithiasis in rats.  

PubMed

Berberis vulgaris is a widely used plant for the treatment of urolithiasis. To evaluate its antiurolithic potential, the crude aqueous-methanol extract of Berberis vulgaris root bark (Bv.Cr) was tested in an animal model of urolithiasis, developed in male Wistar rats by adding 0.75% ethylene glycol in drinking water. Bv.Cr (50 mg/kg) inhibited CaOx crystal deposition in renal tubules and protected against associated changes including polyuria, weight loss, impaired renal function and the development of oxidative stress in kidneys. Activity-guided fractionation revealed the concentration of antiurolithic constituent(s) mainly in the aqueous fraction. These data, indicating the presence of antiurolithic activity in Berberis vulgaris root bark, rationalize its medicinal use for the treatment of urolithiasis. PMID:20564494

Bashir, Samra; Gilani, Anwar H; Siddiqui, Anwar A; Pervez, Shahid; Khan, Saeed R; Sarfaraz, Noor Jehan; Shah, Abdul Jabbar

2010-08-01

40

Identification and characterization of microsatellites from calafate (Berberis microphylla, Berberidaceae)1  

PubMed Central

• Premise of the study: Southern barberry or calafate (Berberis microphylla) is a shrub species endemic to the Patagonian region of South America that is used for human consumption. The fruit is very rich in vitamin C and anthocyanins and has a very high antioxidant capacity. There have been only a few genetic studies of this and other closely related species. • Methods and Results: Here we present the first 18 microsatellite markers of B. microphylla that were characterized using 66 accessions of calafate from Patagonia. On average, they had 7.6 alleles per marker, with an expected heterozygosity of 0.688. The informativeness of these markers was also evaluated in another 15 Berberis species, including most of the native and endemic Chilean species. • Conclusions: The results confirm that these new simple sequence repeat markers are very polymorphic and potentially useful in genetic studies in any species of the genus Berberis. PMID:25202561

Varas, Benjamín; Castro, María H.; Rodriguez, Roberto; von Baer, Dietrich; Mardones, Claudia; Hinrichsen, Patricio

2013-01-01

41

A pharmacological study on Berberis vulgaris fruit extract.  

PubMed

Berberis vulgaris fruit (barberry) is known for its antiarrhythmic and sedative effects in Iranian traditional medicine. The effects of crude aqueous extract of barberry on rat arterial blood pressure and the contractile responses of isolated rat aortic rings and mesenteric bed to phenylephrine were investigated. We also examined effect of the extract on potassium currents recorded from cells in parabrachial nucleus and cerebellum rejoins of rat brain. Administration of the extract (0.05-1 mg/100 g body weight of rat) significantly reduced the mean arterial blood pressure and heart rate in anaesthetized normotensive and desoxycorticosteron acetate-induced hypertensive rats in a dose-dependent manner. Concentration-response curves for phenylephrine effects on isolated rat aortic rings and the isolated mesenteric beds in the presence of the extract were significantly shifted to the right. Application of the extract (1-50 microg/ml) shifted the activation threshold voltage to more negative potentials, leading to an enhancement in magnitude of the outward potassium current recorded from cells present in rat brain slices of parabrachial nucleus and cerebellum. This effect on potassium current may explain the sedative and neuroprotective effects of barberry. The present data support the hypothesis that the aqueous extract of barberry has beneficial effects on both cardiovascular and neural system suggesting a potential use for treatment of hypertension, tachycardia and some neuronal disorders, such as epilepsy and convulsion. PMID:15993555

Fatehi, Mohammad; Saleh, Tarek M; Fatehi-Hassanabad, Zahra; Farrokhfal, Khadige; Jafarzadeh, Mostafa; Davodi, Samaneh

2005-10-31

42

Anticonvulsant effect of Berberis integerrima L. root extracts in mice.  

PubMed

Berberis integerrima is a member of Berberidaceae family. Berberine is one of the main constituents of this plant, having neuroprotective effect on central nervous system diseases. In this study, the anticonvulsant activity of methanolic extract, and hydromethanolic fraction, and chloroform fraction of B integerrima was assessed. The anticonvulsant effect of B integerrima was investigated using both pentylenetetrazole (PTZ) and maximal electroshock (MES)-induced seizure models. The LD50 value of the methanolic extract was 302.676 mg/kg. In the PTZ test, methanolic extract (140 and 200 mg/kg, i.p., p<0.01), hydromethanolic fraction (200 mg/kg, p<0.01), and chloroform fraction (200 mg/kg, p<0.01) increased the onset time of hind limb tonic extensions (HLTEs). The protective effect against mortality (convulsion survivors/animals tested) was 2/8 in methanolic extract, and 3/8 in hydromethanolic fraction at a dose of 200 mg/kg and in chloroform fraction at a dose of 140 mg/kg. In the MES test, this plant did not display any significant effect in reducing HLTE duration. According to phytochemical screening, methanolic extract contained alkaloids and tannins. The present study, conducted in mice, indicated that B integerrima has anticonvulsant activity in PTZ-induced seizures. It is concluded that B integerrima may be useful in petit mal epilepsy. PMID:23433050

Hosseinzadeh, Hossein; Ramezani, Mohammad; Shafaei, Hojjat; Taghiabadi, Elahe

2013-02-01

43

Flavonols, alkaloids, and antioxidant capacity of edible wild berberis species from patagonia.  

PubMed

There are 20 species of the Berberidaceae family described in Chile, whose fruits are edible and show high anthocyanin and hydroxycinnamic acid levels. Berberis microphylla G. Forst, commonly known as calafate, is the most extensively distributed. Flavonols and alkaloids in seed, pulp, skin, and whole calafate berry extracts and other Berberis were studied using HPLC-DAD-ESI-MS/MS and HPLC with fluorescence detector. Berry samples from different locations in Chilean Patagonia, including different phenological stages, were systematically addressed. Results were compared with other organs of the plant and with other Berberis species. Total flavonol concentration in calafate (n = 65) was 1.33 ± 0.54 ?mol/g. Glycosyl metabolites of quercetin and isorhamnetin were the most abundant. Similar profiles were observed in calafate from distinct locations, but important differences were observed for the other edible Berberis species. Calafate pulp and skin have higher flavonol concentrations than seeds, and the maturation process reduced its levels. TEACCUPRAC and TEACABTS of whole calafate extracts and fractions are also explored. Finally, only berberine was detected in the fruit (0.001%), mainly in seeds. Results contribute to the promotion of this berry as a superfruit from Patagonia. PMID:25495577

Ruiz, Antonieta; Zapata, Moises; Sabando, Constanza; Bustamante, Luis; von Baer, Dietrich; Vergara, Carola; Mardones, Claudia

2014-12-24

44

AFLP identification of Berberis thunbergii cultivars, inter-specific hybrids, and their parental species  

Microsoft Academic Search

SUMMARY Cultivars of Berberis thunbergii DC. are among the most popular garden shrubs in the United States. Using 174 amplified fragment length polymorphism (AFLP) markers we were able to differentiate between 43 B. thunbergii cultivars and analyse the genetic similarity of 62 B. thunbergii genotypes, B. julianae, B. koreana, B. vulgaris and B. vulgaris 'Atropurpurea'. Seven different accessions of B.

JESSICA D. LUBELL; MARK H. BRAND; JONATHAN M. LEHRER

2008-01-01

45

Production of Uredia and Telia of Puccinia graminis on Berberis vulgaris  

Microsoft Academic Search

HITHERTO, in Puccinia graminis Pers., the uredial and telial stages have regularly occurred on the gramineous hosts, and the pycnial (spermagonial) and æcial stages on the common barberry (Berberis vulgaris L.) or on closely-related hosts : no deviation from this host relationship has ever been reported. In the course of greenhouse studies, in which barberry plants were infected with pure

Margaret Newton; Thorvaldur Johnson

1937-01-01

46

Insect-mediated reproduction of systemic infections by Puccinia arrhenatheri on Berberis vulgaris  

Microsoft Academic Search

Summary • Witches' brooms on Berberis vulgaris are induced by a systemically infecting rust fungus, Puccinia arrhenatheri. These witches' brooms bear yellow discolored leaves on which the fungus exposes its gametes in a sugary nectar. During the spermatial stage of the fungus the infected leaves emit a strong, flowery scent. • An exclusion-experiment was used to evaluate whether fungal reproductive

Andreas Naef; Bitty A. Roy; Roman Kaiser; Rosmarie Honegger

2002-01-01

47

Mechanism of seedlessness in Iranian seedless barberry ( Berberis vulgaris L. var. asperma)  

Microsoft Academic Search

Species of barberry (Berberis vulgaris L. var. asperma) is cultivated in arid and semi arid areas of Iran (South Khorasan province). It is widely used as a food additive. Fruits of this species are seedless, while wild type barberries produce seeds in the same area. In this study, we investigated the mechanism of seedlessness in seedless barberry by pollen viability

A. Ebadi; M. Rezaei; R. Fatahi

2010-01-01

48

Intra- and interpopulation variability and taxonomic status of Berberis croatica Horvat  

Microsoft Academic Search

Morphological and anatomical traits of Berberis croatica Horvat that would facilitate its separation from the two related taxa, B. aetnensis C. Presl and B. vulgaris L., and their ITS (Internal Transcribed Spacers of nuclear ribosomal DNA) relationships were analyzed. Results show that B. croatica differs from the two related taxa in the following characteristics: plant height, blade width, petiole length,

K. Karlovi?; D. Kremer; Z. Liber; Z. šatovi?; I. Vršek

2009-01-01

49

Inhibition and dissolution of calcium oxalate crystals by Berberis Vulgaris-Q and other metabolites  

Microsoft Academic Search

Inhibition and dissolution of calcium oxalate crystals in solutions containing a homoeopathic medicine Berberis Vulgaris-Q, amino acids such as aspartic acid, glutamic acid, ?-keto glutaric acid, a naturally occurring inhibitor and juices of some fruits of citrus group such as lemon, orange and mausammi have been studied. Considerable solubility was observed and found that in each case solubility changes with

Ishwar Das; S. K Gupta; V. N Pandey; Shoeb A Ansari

2004-01-01

50

Treatment of Cutaneous Leishmaniasis in Murine Model by Alcoholic Extract of Berberis vulgaris  

Microsoft Academic Search

In order to evaluate the effect of Berberis vulgarisextract on the experimental ulcers of cutaneous leishmani- asis (CL) on Balb\\/c mice, a study was undertaken over a 12 months period. Forty Balb\\/c mice were divided into 2 main groups A and B. Each main group in turn was divided into 5 sub groups of 4 mice and each sub group

A Fata; H Rakhshandeh; F Berenji

2006-01-01

51

Controlling Japanese barberry ( Berberis thunbergii DC) in southern New England, USA  

Microsoft Academic Search

Dense Japanese barberry (Berberis thunbergii) stands have spread beyond manicured landscapes and are associated with a paucity of both tree regeneration and herbaceous plants in some forest stands. Studies over 2 years evaluated the effectiveness of various treatment alternatives to control barberry. A total of 375 barberry clumps at three study areas were selected for the first study in 2006.

Jeffrey S. Ward; Thomas E. Worthley; Scott C. Williams

2009-01-01

52

Detailed pharmacognostical studies on Berberis aristata DC plant  

PubMed Central

Background: Berberis aristata DC (Berberidaceae) commonly known in Hindi as “D?ruhaldi” and “Citra,” is an important medicinal herb native to Northern Himalaya region. The plant is used traditionally in Indian system of medicine as an antibacterial, antiperiodic, antidiarrheal and anticancer and it is also used in the treatment of ophthalmic infections. Its root, stem and leaves also find their use in treatment of various ailments and hence is used extensively in Ayurveda. Materials and Methods: Samples of the whole plants of B. aristata were collected and identified. Hand and microtome sections were taken, stained and mounted and the cell content and cell wall structure were studied according to the method described by Kay and Johansen. Representative sketches were made with the help of camera Lucida. Methods for determining the quantitative values were the same as described elsewhere. For fluorescence analysis, the powder of the root, stem and leaf were examined under ultraviolet light. Total ash, acid insoluble ash and water-soluble ash values and water- and alcohol-soluble extractives were determined. Results: The detailed investigations carried on the pharmacognosy of the root; stem and leaf of B. aristata have brought out some salient diagnostic features, which allow one to differentiate it from other substitutes and or adulterants. The determination of quantitative values, fluorescence analysis and the use of lycopodium spore analysis has specifically contributed to this differentiation. Conclusion: From the foregoing observation on the pharmacognosy of root, stem and leaf of B. aristata DC, the salient diagnostic characters of three parts have been presented, which can allow one to differentiate it from other substitutes and or adulterants. PMID:24991073

Rathi, Bhawana; Sahu, Juhi; Koul, Sameksha; Kosha, R. L.

2013-01-01

53

Chapter 7.11 Lam6 Oxide Rainbow Etcher  

E-print Network

Chapter 7.11 Lam6 Oxide Rainbow Etcher (lam6) (584) 1.0 Equipment Purpose 1.1 Lam6 is an automatic, cassette-to-cassette, plasma etcher (Rainbow 4520). This tool is currently dedicated to etching silicon and severely impact consequent etch processes. 3.0 Applicable Documents 3.1 Rainbow 4520 Series System

Healy, Kevin Edward

54

Inhibition and dissolution of calcium oxalate crystals by Berberis Vulgaris-Q and other metabolites  

NASA Astrophysics Data System (ADS)

Inhibition and dissolution of calcium oxalate crystals in solutions containing a homoeopathic medicine Berberis Vulgaris-Q, amino acids such as aspartic acid, glutamic acid, ?-keto glutaric acid, a naturally occurring inhibitor and juices of some fruits of citrus group such as lemon, orange and mausammi have been studied. Considerable solubility was observed and found that in each case solubility changes with time or concentration periodically. Chelation may be one of the reasons for inhibition.

Das, Ishwar; Gupta, S. K.; Pandey, V. N.; Ansari, Shoeb A.

2004-07-01

55

Effect of external calcium on the control of stamen movement in Berberis vulgaris L  

Microsoft Academic Search

The mechanical stimulation of the sensitive internal lower part of Berberis vulgaris stamen resulted in its rapid bending.\\u000a In the present study we have examined the influence of external Ca2+ concentration on stamen movement. The external Ca2+ reduced the extent of the response and the effect was dependent on Ca2+ concentration and duration of the treatment. Addition of calcium ionophore

Z. Lechowski; J. Bia?czyk

1992-01-01

56

Antihistaminic and anticholinergic activity of barberry fruit ( Berberis vulgaris) in the guinea-pig ileum  

Microsoft Academic Search

Barberry (Berberis vulgaris) is a well known medicinal plant in Iran and has also been used as food. The antihistaminic and anticholinergic activity of aqueous extract of barberry fruits were investigated on isolated guinea-pig ileum, and dose–response curves of histamine and acetylcholine with and without extract were plotted. The pA2 values for antihistaminic activity of extract and dexchlorpheniramine were calculated

F Shamsa; A Ahmadiani; R Khosrokhavar

1999-01-01

57

Berberis Fruit Extract and Biochemical Parameters in Patients With Type II Diabetes  

PubMed Central

Background: Diabetes mellitus is a common medical problem. There is in fact a growing body of literature on plants used for the treatment of diabetes. Plant materials attracted considerable interest of scientists. In this respect, in the past few years, attempts were made to use natural plant products for the treatment of patients with diabetes. Objectives: The aim of this study was to investigate the effect of Berberis fruit extract to achieve glycemic control in patients with Type II diabetes. Materials and Methods: This study was performed between July 2010 and April 2013. Thirty patients of type II diabetes admitted to Ayatollah Rohhani hospital were recruited. Patients’ sera were collected for the assessment of glucose and HbA1c values. Biochemical analyses were performed before and after treatment by Berberis fruit extract. Biochemical parameters were measured by spectrophotometric method (Jenway uv/vis, 6505 model, Dunmow, UK). Glucose level was measured by glucose oxidase method kit (Pars Azmoon, Tehran, IR Iran). Serum total cholesterol and triglycerides were measured using standard biochemical kits (Pars Azmoon, Tehran, Iran). Blood glycated hemoglobin level was measured by using Elisa kit (Bioassay technology laboratory, Elisa kit). Experiments were performed in triplicate in at least three separate experiments. Results: Our findings demonstrated that patients with type 2 diabetes who received barberry fruit had significant reduction in serum glucose to 136.15 ± 32.8 mg/dL and decreased HbA1c levels to 7.07 ± 1.21 mg/dL, during the 8 weeks of study. Conclusions: This investigation revealed that Berberis fruit extract has beneficial metabolic effects in patients with type II diabetes. Barberry may improve glucose catabolism via glycolysis pathway, stimulate insulin secretion or improve insulin function and finally decrease glucose uptake. Our results indicated that Berberis fruit regulates glucose metabolism in patients with type 2 diabetes. PMID:24872938

Moazezi, Zolikha; Qujeq, Durdi

2014-01-01

58

Croatian barberry ( Berberis croatica Horvat): a new source of berberine—analysis and antimicrobial activity  

Microsoft Academic Search

By using HPLC\\/UV–VIS, Croatian barberry (Berberis croatica Horvat) was found to be a new source of the bioactive alkaloid berberine. Comparison of berberine content in roots, leaves,\\u000a and twigs between wild specimens of B. croatica and B. vulgaris collected in Croatia showed that the roots of both species contained the highest berberine content (B. croatica 1.120–1.217%; B. vulgaris 0.805–1.424%), followed by twigs (B. croatica

Ivan Kosalec; Barbara Gregurek; Dario Kremer; Marijana Zovko; Krešimir Sankovi?; Ksenija Karlovi?

2009-01-01

59

Effects of Berberis aristata/Silybum marianum association on metabolic parameters and adipocytokines in overweight dyslipidemic patients.  

PubMed

Nutraceuticals and functional foods have attracted considerable interest as potential alternative therapies for treatment of different cardiovascular disorders and insulin resistance. We evaluated the efficacy of a combination of Berberis Aristata/Silybum Marianum extract (Berberol®) in a sample of overweight, dyslipidemic patients at low cardiovascular risk. We enrolled 105 Caucasian, euglycemic, overweight, dyslipidemic patients, of either sex. At baseline all patients underwent a 6 months run-in period during which they followed an adequate diet and practiced physical activity. At the end of the run-in period, patients were randomised to take placebo or a combination of Berberis aristata/Silybum marianum, 1 tablet during the lunch and 1 tablet during the dinner, for three months, in a double-blind, placebo-controlled design. Berberis aristata/Silybum marianum and placebo were then interrupted for 2 months (wash-out period), and all patients continued with only diet and physical activity. At the end of the wash-out period, patients re-started Berberis aristata/Silybum marianum or placebo twice a day for further 3 months. We evaluated during the run-in period, at randomisation, before and after the wash-out period these parameters: body weight and BMI, fasting plasma glucose, lipid profile, insulin resistance, retinol binding protein-4 (RBP-4), adiponectin (ADN), resistin. Total cholesterol, LDL-C, and Tg decreased, and HDL-C increase after 3 months of Berberis aristata/Silybum marianum, both compared to baseline and placebo. Berberis aristata/Silybum marianum decreased fasting plasma insulin, and HOMA-IR, both compared to baseline and to placebo. Moreover, there was a decrease of RBP-4, and resistin, and an increase of ADN after 3 months of Berberis aristata/Silybum marianum. All these positive effects disappeared after the wash-out period, and re-appeared after the re-introduction of the drug. We observed a significant correlation between HOMA-index decrease and resistin, and RBP-4 decrease, and between HOMA-index decrease and ADN increase in Berberis aristata/Silybum marianum group, but not in placebo group. Berberis aristata/Silybum marianum fixed combination seems to be safe and effective in improving lipid profile, but also in improving insulin resistance and adipocytokines levels. PMID:24152839

Derosa, G; Bonaventura, A; Bianchi, L; Romano, D; D' Angelo, A; Fogari, E; Maffioli, P

2013-01-01

60

Copyright 2011 Jennifer Lam-Anh Gosselin  

E-print Network

-like pattern in the survival curves of juvenile hatchery rainbow trout (Oncorhynchus mykiss). In Chapter 4 Survival: Examples from a model species (Poecilia reticulata) and salmonid species (Oncorhynchus spp: Examples from a model species (Poecilia reticulata) and salmonid species (Oncorhynchus spp.) Jennifer Lam

Washington at Seattle, University of

61

“Getting stuck with LAM”: patients perspectives on living with Lymphangioleiomyomatosis  

PubMed Central

Background Lymphangioleiomyomatosis (LAM) is a rare, progressive lung disease that affects almost exclusively women and is most often diagnosed before menopause. The main symptom of LAM is shortness of breath. LAM patients’ perceptions of how the disease impacts their lives is largely unknown, but such information could be useful to generate patient reported outcome measures for use in drug trials (or other research studies) and to formulate interventions aimed at easing the burdens LAM imposes on patients. Objective To capture patients’ perceptions of how LAM affects their lives. Methods We used reflexive team analysis to analyze transcripts from semi-structured focus groups conducted with LAM patients at LAMposium 2013. We sought to determine what patients perceive as the primary symptoms of LAM and how the disease affects them in their daily lives. Results The 37 participants described seven primary symptoms of LAM and five common psychological experiences from living with the disease. Shortness of breath and low energy (or fatigue) dominated the symptomatic picture; cough, sensations in the chest, difficulty sleeping, gastrointestinal issues, and mild cognitive difficulties were less common. The common psychological experiences participants reported included frustration, worry, loss of identity, embarrassment, and in some participants, a healthy defiance against the disease. Conclusions Patients perceive the physical symptoms from LAM to be intrusive and limiting. Women living with LAM are frustrated by their physical limitations, and they worry about what the future will be like if the disease progresses. Therapeutic interventions should take aim at improving these perceptions. PMID:24885591

2014-01-01

62

A new isoquinoline alkaloid with anti-microbial properties from Berberis jaeschkeana Schneid. var. jaeschkeana.  

PubMed

One new isoquinoline alkaloid named berberidione (1) along with four new source alkaloids berberine (2), palmatine (3), jatrorrhizine (4) and chondrofoline (5) and three new source non-alkaloids syringic acid (6), ?-sitosterol (7) and stigmasterol (8) was isolated and characterised from different fractions of Berberis jaeschkeana Schneid var. jaeschkeana. All the structures were determined from 1D and 2D spectroscopic data. Crude extract, sub-fractions and isolated compounds showed excellent anti-microbial properties. The toxicity level for the alkaloids was found to be very low on THP-1 cells. PMID:25424893

Alamzeb, Muhammad; Khan, M Rafiullah; Ali, Saqib; Khan, Ashfaq Ahmad

2014-11-25

63

The Effect of Light, Temperature, pH and Species on Stability of Anthocyanin Pigments in Four Berberis Species  

Microsoft Academic Search

2 Abstract: The anthocyanin pigment was extracted from the four different Berberis plant (B. khorasanica, B. integerrima, B. orthobotrys, and B. vulgaris) using the soaking and wetting in Ethanol (1% acidified). The extracted anthocyanin pigments then were exposed to number of environmental conditions, which could destabilize the anthocyanin molecules. These environmental conditions were included three different pHs (0, 1.5 and

2006-01-01

64

Role of Berberis spp. as alternate hosts in generating new races of Puccinia graminis and P. striiformis  

Microsoft Academic Search

The common barberry and several other Berberis spp. serve as the alternate hosts to two important rust pathogens of small grains and grasses, Puccinia graminis and P. striiformis. Barberry eradication has been practiced for centuries as a means to control stem rust. Diverse virulence variations have\\u000a been observed in populations of P. graminis f. sp. tritici that were associated with

Yue Jin

2011-01-01

65

Century-old Mystery of Puccinia striiformis Life History Solved with the Identification of Berberis as an Alternate Host  

Technology Transfer Automated Retrieval System (TEKTRAN)

The life history of Puccinia striiformis remains a mystery because the alternate host has never been found. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P...

66

Induction of tetraploidy in meristematically active seeds of Japanese barberry ( Berberis thunbergii var. atropurpurea) through exposure to colchicine and oryzalin  

Microsoft Academic Search

Japanese barberry (Berberis thunbergii DC) is an invasive shrub, widely naturalized across the United States, whose numerous cultivars remain an important horticultural commodity. Maintaining this crop for the future necessitates the development of sterile clones. Exposure to the mitotic inhibitors colchicine and oryzalin is a traditional method for inducing tetraploidy in breeding lines as a precursor to creating sterile genotypes.

Jonathan M. Lehrer; Mark H. Brand; Jessica D. Lubell

2008-01-01

67

Fruit selection by birds in relation to fruit abundance and appearance in the naturalised shrub Berberis darwinii  

Microsoft Academic Search

The effects of three forms of fruit damage were tested on avian selection of the fleshy fruits of Darwin’s barberry (Berberis darwinii) from plastic trays, and compared with the abundance of fruits and their removal rates from nearby bushes, in December-January near Dunedin, New Zealand. Ripe fruits were removed from bushes by four bird species: blackbird (Turdus merula), song thrush

R. B. Allen; W. G. Lee

1992-01-01

68

Structure and Dynamics of Populations of Japanese Barberry (Berberis Thunbergii DC.) in Deciduous Forests of New Jersey  

Microsoft Academic Search

Japanese barberry, Berberis thunbergii DC., has become a prominent exotic species in deciduous forests throughout the eastern and midwestern US. Populations range from small plants occurring at low densities to dense, impenetrable thickets of plants with up to 40 stems\\/individual. A study was undertaken at Morristown National Historical Park in New Jersey to document plant densities, plant size, recruitment through

Joan G. Ehrenfeld

1999-01-01

69

Aqueous extract of Berberis integerrima root improves renal dysfunction in streptozotocin induced diabetic rats  

PubMed Central

Objective: Barberry root extract contains various alkaloids that are considered as antioxidants. Beneficial effect of aqueous extract of Berberis integerrima root (AEBIR) was evaluated for renal function in diabetic rats induced by STZ. Material and Methods: Diabetes was induced by i.p. injection of streptozotocin (65 mg/kg bw) to rats, after 15 h of fasting. Diabetic rats were randomly grouped and treated daily with AEBIR and glibenclamide by gavage for 42 days. After 6 weeks of study, all the rats were sacrificed and some biochemical parameters of serum and urine were measured and their kidneys tissues were processed for light microscopy. Results: Streptozotocin induced a significant rise in fasting blood glucose, serum creatinine, blood urea nitrogen, urine glucose, urine protein, urine albumin, and water intake and a significant decrease in body weight, serum protein, urine urea, and urine creatinine. There was a significant restoration of these parameters to near normal after administration of the AEBIR and also by glibenclamide (0.6 mg/kg bw). The activity of the extract at dose of 500 mg/kg in all parameters except blood glucose and urine glucose was more than that of the standard drug, glibenclamide (0.6 mg/kg, p.o.). Histopathological changes of kidney samples were comparable with respective control. Conclusion: These results suggested that aqueous extract of Berberis Integerrima root improves renal dysfunction in streptozotocin-induced diabetic rats through controlling blood glucose and renal protective effects. PMID:25050261

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid; Ilkhanipoor, Minoo

2013-01-01

70

Using HPLC to Determination the Composition and Antioxidant Activity of Berberis Vulgaris  

E-print Network

The Berberis vulgaris (BV) plant has been extensively used as a traditional medicine in some part of Asian region especially in Western Asia. The BV fruits is claimed also to have anti-viral activities, and as a treatment for chronic candidiasis, indigestion and parasites. The present study was conducted to evaluate the antioxidative activity, nutritional and antinutritional composition of Berberis vulgaris fruit. The antioxidant activity was determined by using thiobarbituric acid (TBA method). Absolute ethanol, absolute methanol and aqueous solution (distilled water) were used as a solvent in this study. Result shows significantly differences (PBerberis vulgaris also contains nutritional and antinutritional composition namely vitamin C, malic acid and Tannin. Vitamin C and malic acid also shows antioxidative properties. Vitamin C and malic acid also showed antioxidative properties and act synergistically to each other as antioxidants. The presence of antinutritional such as Tannin which can act as a medium to prevent nutritional composition from being absorbed by the body also has been detected. The amount of nutritional and antinutritional detected in 100g of berberry were 11102.81?g ± 2.01 of Vitamin C, 116.03?g ± 1.12 of Malic acid and 20.51?g ± 0.59 of Tannin.

Parichehr Hanachi; Golkho Sh

71

Leaf phenology and seasonal variation of photosynthesis of invasive Berberis thunbergii (Japanese barberry) and two co-occurring native understory shrubs in a northeastern United States deciduous forest  

Microsoft Academic Search

Early leafing and extended leaf longevity can be important mechanisms for the invasion of the forest understory. We compared\\u000a the leaf phenology and photosynthetic characteristics of Berberis thunbergii, an early leafing invasive shrub, and two co-occurring native species, evergreen Kalmia latifolia and late leafing Vaccinium corymbosum, throughout the 2004 growing season. Berberis thunbergii leafed out 1 month earlier than V. corymbosum

Cheng-Yuan Xu; Kevin L. Griffin; W. S. F. Schuster

2007-01-01

72

An antitumor promoter from Moringa oleifera Lam  

Microsoft Academic Search

In the course of studies on the isolation of bioactive compounds from Philippine plants, the seeds of Moringa oleifera Lam. were examined and from the ethanol extract were isolated the new O-ethyl-4-(?-l-rhamnosyloxy)benzyl carbamate (1) together with seven known compounds, 4(?-l-rhamnosyloxy)-benzyl isothiocyanate (2), niazimicin (3), niazirin (4), ?-sitosterol (5), glycerol-1-(9-octadecanoate) (6), 3-O-(6?-O-oleoyl-?-d-glucopyranosyl)-?-sitosterol (7), and ?-sitosterol-3-O-?-d-glucopyranoside (8). Four of the isolates (2,

Amelia P. Guevara; Carolyn Vargas; Hiromu Sakurai; Yasuhiro Fujiwara; Keiji Hashimoto; Takashi Maoka; Mutzuo Kozuka; Yoshohiro Ito; Harukuni Tokuda; Hoyoku Nishino

1999-01-01

73

Lymphangiogenesis-Mediated Shedding of LAM Cell Clusters as a Mechanism for Dissemination in Lymphangioleiomyomatosis  

Microsoft Academic Search

Lymphangioleiomyomatosis (LAM) affects exclusively women of reproductive age, involves the lungs and axial lymphatic system, and is frequently complicated with renal angiomyolipomas. LAM lesions are generated by the proliferation of LAM cells with mutations of one of the tuberous sclerosis complex (TSC) genes. Recent studies indicate that LAM cells can migrate or metastasize to form new lesions in multiple organs,

Toshio Kumasaka; Kuniaki Seyama; Keiko Mitani; Sanae Souma; Satoko Kashiwagi; Akira Hebisawa; Teruhiko Sato; Hajime Kubo; Kiyoshi Gomi; Kazutoshi Shibuya; Yoshinosuke Fukuchi; Koichi Suda

2005-01-01

74

Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine.  

PubMed

Barberry (Berberis vulgaris L. family Berberidaceae) is well known in Iran and various parts of this plant including its root, bark, leaf and fruit have been used as folk medicine. The two decades of research has demonstrated different pharmacological and therapeutic effects of B. vulgaris and its isoquinoline alkaloids (particularly berberine). Studies carried out on the chemical composition of the plant show that the most important constituents of this plant are isoquinoline alkaloids such as berberine, berbamine and palmatine. Berberine represents one of the most studied among the naturally occurring protoberberine alkaloids. In addition to B. vulgaris (barberry), berberine is present in many other plants and is used for the treatment of different diseases. This article reviews the traditional uses and pharmacological effects of total extract and the most active ingredient of B. vulgaris (berberine). PMID:18618524

Imanshahidi, Mohsen; Hosseinzadeh, Hossein

2008-08-01

75

Predictive distribution modeling for rare Himalayan medicinal plant Berberis aristata DC.  

PubMed

Predictive distribution modelling of Berberis aristata DC, a rare threatened plant with high medicinal values has been done with an aim to understand its potential distribution zones in Indian Himalayan region. Bioclimatic and topographic variables were used to develop the distribution model with the help of three different algorithms viz. Genetic Algorithm for Rule-set Production (GARP), Bioclim and Maximum entropy (MaxEnt). Maximum entropy has predicted wider potential distribution (10.36%) compared to GARP (4.63%) and Bioclim (2.44%). Validation confirms that these outputs are comparable to the present distribution pattern of the B. aristata. This exercise highlights that this species favours Western Himalaya. However, GARP and MaxEnt's prediction of Eastern Himalayan states (i. e. Arunachal Pradesh, Nagaland and Manipur) are also identified as potential occurrence places require further exploration. PMID:22471208

Ray, Rajasri; Gururaja, K V; Ramchandra, T V

2011-11-01

76

Effects of Aqueous Extract of Berberis integerrima Root on Some Physiological Parameters in Streptozotocin-Induced Diabetic Rats  

PubMed Central

Diabetes mellitus is a common endocrine disorder. Anti-diabetic agents from natural and synthetic sources are available for the treatment of this disease. Berberis integerrima is a medicinal shrub used in conventional therapy for a number of diseases. The aim of the present study was to investigate the effects of aqueous extract of Berberis integerrima root (AEBI) on some physiological parameters in normal and streptozotocin-induced (STZ-induced) diabetic male Wistar rats. STZ-induced diabetic rats showed significant increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C), creatinine (Cr), urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin while body weight, high density lipoprotein HDL-cholesterol (HDL-C) and total protein levels were significantly decreased compared to normal rats. Treatment of diabetic rats with different doses of aqueous extract of Berberis integerrima root (250 and 500 mg/Kg bw) resulted in a significant decrease in blood glucose, triglycerides, cholesterol, LDL-cholesterol, ALT, AST, ALP, total bilirubin, creatinine and urea while HDL-cholesterol and total protein levels were markedly increased after six weeks compared to untreated diabetic rats. The effects of the AEBI at dose of 500 mg/Kg in all parameters except blood glucose (similar) is more than to the standard drug, glibenclamide (0.6 mg/Kg, p.o.). The results of this study indicate that the tested aqueous extract of Berberis integerrima root possesses hypoglycemic, hypolipidemic and antioxidant effects in STZ-induced diabetic rats. PMID:24250618

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid; Ilkhanipoor, Minoo

2013-01-01

77

Effects of Aqueous Extract of Berberis integerrima Root on Some Physiological Parameters in Streptozotocin-Induced Diabetic Rats.  

PubMed

Diabetes mellitus is a common endocrine disorder. Anti-diabetic agents from natural and synthetic sources are available for the treatment of this disease. Berberis integerrima is a medicinal shrub used in conventional therapy for a number of diseases. The aim of the present study was to investigate the effects of aqueous extract of Berberis integerrima root (AEBI) on some physiological parameters in normal and streptozotocin-induced (STZ-induced) diabetic male Wistar rats. STZ-induced diabetic rats showed significant increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C), creatinine (Cr), urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin while body weight, high density lipoprotein HDL-cholesterol (HDL-C) and total protein levels were significantly decreased compared to normal rats. Treatment of diabetic rats with different doses of aqueous extract of Berberis integerrima root (250 and 500 mg/Kg bw) resulted in a significant decrease in blood glucose, triglycerides, cholesterol, LDL-cholesterol, ALT, AST, ALP, total bilirubin, creatinine and urea while HDL-cholesterol and total protein levels were markedly increased after six weeks compared to untreated diabetic rats. The effects of the AEBI at dose of 500 mg/Kg in all parameters except blood glucose (similar) is more than to the standard drug, glibenclamide (0.6 mg/Kg, p.o.). The results of this study indicate that the tested aqueous extract of Berberis integerrima root possesses hypoglycemic, hypolipidemic and antioxidant effects in STZ-induced diabetic rats. PMID:24250618

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid; Ilkhanipoor, Minoo

2013-01-01

78

Biomass allocation shade tolerance and seedling survival of the invasive speciesBerberis darwinii(Darwin’ s barberry)  

Microsoft Academic Search

Abstract: Berberis darwinii (Berberidaceae) is a serious environmental weed in New Zealand, capable of invading a range of different light environments,from,grazed pasture to intact forest. According to optimal partitioning models, some plants optimise growth under different environmental conditions by shifting biomass allocation among tissue types (e.g. roots, shoots) to maximise the capture of limiting resources (e.g. water, light). We examined

Kate G. Mcalpine; Linley K. Jesson

79

Antileishmanial compounds from Moringa oleifera Lam.  

PubMed

The antileishmanial activity of extracts and phytoconstituents of Moringa oleifera Lam. was investigated in vitro against promastigotes of Leishmania donavani. The 70% ethanolic extract of roots and the methanolic extract of leaves showed moderate inhibitory activity with IC50 values of 83.0 microg/ml and 47.5 microg/ml, respectively. Antileishmanial activity of the methanolic extract of leaves increased upon fractionation, as its ethyl acetate fraction was found to be more active with an IC50 value of 27.5 microg/ml. The most active antileishmanial compound niazinin, a thiocarbamate glycoside isolated from this fraction, showed an IC50 value of 5.25 microM. Results presented in this study indicate that extracts from M. oleifera may be developed as an adjuvant therapy for the treatment of leishmaniasis. PMID:24873031

Kaur, Amandeep; Kaur, Preet Kamal; Singh, Sushma; Singh, Inder Pal

2014-01-01

80

UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kelsie Priest, Lahiru Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam  

E-print Network

Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam UBC Botanical Garden Redevelopment of a project/report". #12;UBC Botanical Garden Redevelopment Detailed Design Report Prepared for UBC CIVIL 446 and UBC Botanical Garden Prepared by CIVIL 446 - Group One Mike Kai Hin Lam # Kelsie Priest # Lahiru

81

Classical closure theory and Lam's interpretation of epsilon-RNG  

NASA Technical Reports Server (NTRS)

Lam's phenomenological epsilon-renormalization group (RNG) model is quite different from the other members of that group. It does not make use of the correspondence principle and the epsilon-expansion procedure. We demonstrate that Lam's epsilon-RNG model is essentially the physical space version of the classical closure theory in spectral space and consider the corresponding treatment of the eddy viscosity and energy backscatter.

Zhou, YE

1995-01-01

82

75 FR 29722 - Foreign-Trade Zone 18-San Jose, CA; Application for Subzone; Lam Research Corporation (Wafer...  

Federal Register 2010, 2011, 2012, 2013

...Jose, CA; Application for Subzone; Lam Research Corporation (Wafer Fabrication Equipment...equipment manufacturing facilities of Lam Research Corporation (Lam), located in Fremont...valves, taps, bearings, stainless steel screws, pulleys, shaft couplings,...

2010-05-27

83

78 FR 3319 - Amendments to Existing Validated End User Authorizations: Advanced Micro Devices China, Inc., Lam...  

Federal Register 2010, 2011, 2012, 2013

...Optical Valley Software Park, Wuhan, Hubei, China 430074. Lam Research Semiconductor...Hi-Tech Development Zone, Wuhan City, Hubei Province, China 430223. Names and Current...Road, Donghu Development Zone, Wuhan, Hubei Province, China 430074. Lam...

2013-01-16

84

Preliminary study about the possible glycemic clinical advantage in using a fixed combination of Berberis aristata and Silybum marianum standardized extracts versus only Berberis aristata in patients with type 2 diabetes  

PubMed Central

Background Berberine is an isoquinoline alkaloid widely used to improve the glucidic and lipidic profiles of patients with hypercholesterolemia, metabolic syndrome, and type 2 diabetes. The limitation of berberine seems to be its poor oral bioavailability, which is affected by the presence, in enterocytes, of P-glycoprotein – an active adenosine triphosphate (ATP)-consuming efflux protein that extrudes berberine into the intestinal lumen, thus limiting its absorption. According to some authors, silymarin, derived from Silybum marianum, could be considered a P-glycoprotein antagonist. Aim The study aimed to evaluate the role played by a possible P-glycoprotein antagonist (silymarin), when added to a product containing Berberis aristata extract, in terms of benefits to patients with type 2 diabetes. Methods The study enrolled 69 patients with type 2 diabetes in suboptimal glycemic control who were treated with diet, hypoglycemic drugs, and in cases of concomitant alterations of the lipid profile, hypolipidemic agents. The patients received an add-on therapy consisting of either a standardized extract of Berberis aristata (titrated in 85% berberine) corresponding to 1,000 mg/day of berberine, or Berberol®, a fixed combination containing the same standardized extract of Berberis aristata plus a standardized extract of Silybum marianum (titrated as >60% in silymarin), for a total intake of 1,000 mg/day of berberine and 210 mg/day of silymarin. Results Both treatments similarly improved fasting glucose, total cholesterol, low-density lipoprotein (LDL) cholesterol, triglyceride, and liver enzyme levels, whereas glycosylated hemoglobin (HbA1c) values were reduced to a greater extent by the fixed combination. Conclusion The association of berberine and silymarin demonstrated to be more effective than berberine alone in reducing HbA1c, when administered at the same dose and in the form of standardized extracts in type 2 diabetic patients. PMID:24277991

Di Pierro, Francesco; Putignano, Pietro; Villanova, Nicola; Montesi, Luca; Moscatiello, Simona; Marchesini, Giulio

2013-01-01

85

Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum.  

PubMed

Barberry (Berberis vulgaris) is a well known medicinal plant in Iran and has also been used as food. The antihistaminic and anticholinergic activity of aqueous extract of barberry fruits were investigated on isolated guinea-pig ileum, and dose response curves of histamine and acetylcholine with and without extract were plotted. The pA2 values for antihistaminic activity of extract and dexchlorpheniramine were calculated (extract; pA2 +/- S.E.M. = 4.50 +/- 0.01[-log C (g/l)]; dexchlorpheniramine; pA2 +/- S.E.M. = 9.36 +/- 0.14[-log C (M)]) and compared with each other. The pA2 values for anticholinergic activity of extract and atropine were also calculated (extract, pA2 +/- S.E.M. = 4.37 +/- 0.03[-log C (g/l)]; atropine, pA2 +/- S.E.M. = 8.99 +/- 0.13[-log C (M)]) and compared. The results indicated antihistaminic and anticholinergic activity of extract that seems to be of the competitive type. PMID:10197751

Shamsa, F; Ahmadiani, A; Khosrokhavar, R

1999-02-01

86

Amplicon pyrosequencing reveals the soil microbial diversity associated with invasive Japanese barberry (Berberis thunbergii DC.).  

PubMed

The soil microbial community acts as a reservoir of microbes that directly influences the structure and composition of the aboveground plant community, promotes plant growth, increases stress tolerance and mediates local patterns of nutrient cycling. Direct interactions between plants and rhizosphere-dwelling microorganisms occur at, or near, the surface of the root. Upon introduction and establishment, invasive plants modify the soil microbial communities and soil biochemistry affecting bioremediation efforts and future plant communities. Here, we used tag-encoded FLX amplicon 454 pyrosequencing (TEFAP) to characterize the bacterial and fungal community diversity in the rhizosphere of Berberis thunbergii DC. (Japanese barberry) from invasive stands in coastal Maine to investigate effects of soil type, soil chemistry and surrounding plant cover on the soil microbial community structure. Acidobacteria, Actinobacteria, Proteobacteria and Verrucomicrobia were the dominant bacterial phyla, whereas fungal communities were comprised mostly of Ascomycota and Basidiomycota phyla members, including Agaricomycetes and Sordariomycetes. Bulk soil chemistry had more effect on the bacterial community structure than the fungal community. An effect of geographic location was apparent in the rhizosphere microbial communities, yet it was less significant than the effect of surrounding plant cover. These data demonstrate a high degree of spatial variation in the rhizosphere microbial communities of Japanese barberry with apparent effects of soil chemistry, location and canopy cover on the microbial community structure. PMID:24118303

Coats, V C; Pelletreau, K N; Rumpho, M E

2014-03-01

87

Possible mechanism of cardiac depressant activity of Berberis orthobotrys roots in isolated rabbit heart.  

PubMed

Berberis orhob, otrvs Bien. ex Aitch. (B.o.) has been reported to have antihypertensive effect in different experimental models. The aim of present study was to evaluate the possible antihypertensive mechanism. Aqueous methanolic extract of B.o. roots and its various fractions namely (ethyl acetate, n-butanol or aqueous) in different concentrations (10 ng/mL, 100 ng/mL, I pg/mL, 10 pg/mL, 100 pg/mL ) were evaluated in isolated perfused rabbit heart to assess their effect on force of contraction, HR and perfusion pressure. The crude extract of B.o. and its fractions exhibited a significant decrease in heart rate, contractility and perfusion pressure of isolated rabbit heart, however, butanolic fraction produced more prominent effect and was selected for further study. The effects of butanol fraction were not blocked by atropine (10' M) in isolated perfused heart. However, butanol fraction significantly blocked the effects of adrenaline (10' M). It is therefore conceivable that cardiac depressant activity of B.o. butanol fraction might be due to the presence of certain 3-blocking agents which might be responsible for antihypertensive effect However, further experiments are required to isolate the active compound(s) and elucidate exact mechanism of action. PMID:25272893

Alamgeer; Akhtar, Muhammad Shoaib; Jabeen, Qaiser; Bashir, Sajid; Malik, Muhammad Nasir Hayat; Karim, Sabeha; Mushtaq, Muhammad Naveed; Rasool, Shahid; Latif, Fozia; Tabbasum, Nazia; Khan, Abdul Qayyum; Ahsan, Haseeb; Khan, Wasim; Javed, Ibrahim

2014-01-01

88

Antioxidant and anti-inflammatory compounds in the popular landscape plant Berberis thunbergii var. atropurpurea.  

PubMed

Berberis thunbergii var, atropurpurea. DC is one of the popular landscape plants in the USA, but until now lacked report on its chemical composition and biological properties. In this study, the antioxidant and anti-inflammatory activities of the methanolic extract and pure isolates of B. thunbergii var. atropurpurea, Crimson Pygmy, roots were evaluated using established bioassay procedures. The methanolic extract gave an absorbance value of 0.44 at 250 microg/mL concentration in the MTT assay. In addition, the extract inhibited lipid peroxidation (LPO) by 93% and the cyclooxygenase enzymes, COX-1 by 54 and COX-2 by 34%, at 100 microg/mL concentration. Therefore, a bioactivity-guided purification was carried out yielding pure isolates, out of which compounds 3-6 inhibited LPO by 34-66% at 100 microg/mL concentration. Similarly, compounds 1-6 inhibited COX-1 and -2 by 24-65 and 23-43% at 25 microg/mL concentration, respectively. This is the first report of the chemical constituents and biological activities of this plant. PMID:23513718

Zhang, Chuan-Rui; Schutzki, Robert E; Nair, Muraleedharan G

2013-02-01

89

Voltammetric determination of antioxidant character in Berberis lycium Royel, Zanthoxylum armatum and Morus nigra Linn plants.  

PubMed

The antioxidant activity potential of three different plant extracts was investigated against superoxide anion radical while employing cyclic voltammetry technique. The plants Berberis lyceum Royle, Morus nigra Linn and Zanthoxylum armatum were selected because of their potential use in the traditional medicine. The voltammetric response of the electrochemically generated superoxide anion radial in DMSO was monitored in the absence and presence of the plat extracts. The decrease in the current was interpreted in terms of antiradical activity of the added extract. The thermodynamic feasibility of the radical scavenging by extracts was accounted in terms of antioxidant activity coefficient (K(ao)) and standard Gibbs free energy (?G(o)). The values of K(ao) and ?G(o) ranged from 1.0 x 102 to 57 x 102 L(-1) and -18 to -27 kJmol(-1), respectively. The possible mechanism of the antioxidant reaction was regarded as E(r)C(i) mechanism i.e. reversible electron transfer followed by hydrogen atom transfer- an irreversible chemical reaction. PMID:22713934

Ahmed, Safeer; Shakeel, Faria

2012-07-01

90

Effects of Zataria multiflora and Carum carvi essential oils and hydroalcoholic extracts of Passiflora incarnata, Berberis integerrima and Crocus sativus on rat isolated uterus contractions  

Microsoft Academic Search

Spontaneous abortion can be a problem in pregnancy. Consumption of some plant material might be a cause, while other plant materials are being used in folk medicine for treatment of this condition. The aim of this study was to look for effects of essential oils of Zataria multiflora and Carum carvi and hydroalcoholic extracts of Passiflora incarnata, Berberies integerrima and

Hassan Sadraei; Alireza Ghannadi; Maryam Takei-bavani

2003-01-01

91

Berberis libanotica Ehrenb Extract Shows Anti-Neoplastic Effects on Prostate Cancer Stem/Progenitor Cells  

PubMed Central

Cancer stem cells (CSCs), including those of advanced prostate cancer, are a suggested reason for tumor resistance toward conventional tumor therapy. Therefore, new therapeutic agents are urgently needed for targeting CSCs. Despite the minimal understanding of their modes of action, natural products and herbal therapies have been commonly used in the prevention and treatment of many cancers. Berberis libanotica Ehrenb (BLE) is a plant rich in alkaloids which may possess anti-cancer activity and a high potential for eliminating CSCs. We tested the effect of BLE on prostate cancer cells and our data indicated that this extract induced significant reduction in cell viability and inhibited the proliferation of human prostate cancer cell lines (DU145, PC3 and 22Rv1) in a dose- and time-dependent manner. BLE extract induced a perturbation of the cell cycle, leading to a G0-G1 arrest. Furthermore, we noted 50% cell death, characterized by the production of high levels of reactive oxidative species (ROS). Inhibition of cellular migration and invasion was also achieved upon treatment with BLE extract, suggesting a role in inhibiting metastasis. Interestingly, BLE extract had a major effect on CSCs. Cells were grown in a 3D sphere-formation assay to enrich for a population of cancer stem/progenitor cells. Our results showed a significant reduction in sphere formation ability. Three rounds of treatment with BLE extract were sufficient to eradicate the self-renewal ability of highly resistant CSCs. In conclusion, our results suggest a high therapeutic potential of BLE extract in targeting prostate cancer and its CSCs. PMID:25380390

Eid, Assaad; Daoud, Georges; Hosry, Leina; Monzer, Alissar; Mouhieddine, Tarek H.; Hamade, Aline; Najjar, Fadia; Abou-Kheir, Wassim

2014-01-01

92

Sexual structures and recombination of the wheat rust fungus Puccinia striiformis on Berberis vulgaris.  

PubMed

An isolate of the basidiomycete Puccinia striiformis, which causes yellow (stripe) rust on wheat, was selfed on the newly discovered alternate host, Berberis vulgaris. This allowed a study of the segregation of molecular markers and virulence in the progeny isolates, and of the development of fungal sexual structures and spore forms. Pycnia and aecia were obtained after inoculation of B. vulgaris with basidiospores resulting from germinating teliospores from infected wheat leaves. Subsequent inoculation of wheat with aeciospores from bulked aecia resulted in 16 progeny isolates of the S1 generation. Genotyping with 42 simple sequence repeat (SSR) markers confirmed a parental origin of progeny isolates. Of the 42 analyzed loci, 15 were heterozygous in the parental isolate and 14 revealed segregation in the progenies. This resulted in 11 new multilocus genotypes (MLGs), which confirmed segregation following sexual reproduction. Additionally, parental and progeny isolates were phenotyped using a genetic stock of wheat genotypes representing 21 resistance genes. All S1 progeny isolates had virulence for 14 out of 15 loci where the parental isolate was virulent. This was consistent with the hypothesis that virulence in plant pathogens is often recessive to avirulence, i.e., only expressed in a homozygous state. Furthermore, no segregation was observed for five out of six loci, for which the parental isolate had an avirulent phenotype. The results for one of the two segregating virulence/avirulence loci suggested that the parental isolate was heterozygous with Avr alleles resulting in different but clearly avirulent phenotypes. The other locus indicated that additional genes modifying the phenotypic expression of avirulence were involved. PMID:25042987

Rodriguez-Algaba, Julian; Walter, Stephanie; Sørensen, Chris K; Hovmøller, Mogens S; Justesen, Annemarie F

2014-09-01

93

Aqueous extract of dried fruit of Berberis vulgaris L. in acne vulgaris, a clinical trial.  

PubMed

Berberis vulgaris L. (barberry) is a very well-known herb in traditional medicine. Apart from its anti-inflammatory and antibacterial properties, the antilipogenic effect of barberry on the sebaceous glands in animals may further suggest it could be employed as an anti-acne agent. This study examined the effect of oral aqueous extract of barberry on acne vulgaris. Adolescents aged 12-17 years with moderate to severe acne vulgaris were randomly given oral gelatin capsules containing either aqueous extract of dried barberry (600 mg daily for 4 weeks, n = 25) or placebo (n = 24). Counts of facial noninflamed, inflamed, and total acne lesions, as well as the Michaelson's acne severity score were documented at baseline and at weeks 2 and 4. Both groups were comparable in terms of the patients' characteristics and baseline variables. After 4 weeks, the mean number of noninflamed, inflamed, and total lesions as well as mean Michaelson's acne severity score declined significantly by 43.25 ± 10.88% (median: 42.11%), 44.53 ± 11.78% (median: 45.45%), 44.64 ± 8.46% (median: 46.15%), and 44.38 ± 8.25% (median: 44.07%), respectively, among the extract receivers (p <.001 for all the changes). Similar changes were not significant in the placebo group. No notable complication or side effect was reported in relation to barberry. In conclusion, oral aqueous extract of dried barberry is a safe, well-tolerated, and effective choice in teenagers with moderate to severe acne vulgaris. PMID:23038982

Fouladi, Rohollah F

2012-12-01

94

Berberis libanotica Ehrenb extract shows anti-neoplastic effects on prostate cancer stem/progenitor cells.  

PubMed

Cancer stem cells (CSCs), including those of advanced prostate cancer, are a suggested reason for tumor resistance toward conventional tumor therapy. Therefore, new therapeutic agents are urgently needed for targeting CSCs. Despite the minimal understanding of their modes of action, natural products and herbal therapies have been commonly used in the prevention and treatment of many cancers. Berberis libanotica Ehrenb (BLE) is a plant rich in alkaloids which may possess anti-cancer activity and a high potential for eliminating CSCs. We tested the effect of BLE on prostate cancer cells and our data indicated that this extract induced significant reduction in cell viability and inhibited the proliferation of human prostate cancer cell lines (DU145, PC3 and 22Rv1) in a dose- and time-dependent manner. BLE extract induced a perturbation of the cell cycle, leading to a G0-G1 arrest. Furthermore, we noted 50% cell death, characterized by the production of high levels of reactive oxidative species (ROS). Inhibition of cellular migration and invasion was also achieved upon treatment with BLE extract, suggesting a role in inhibiting metastasis. Interestingly, BLE extract had a major effect on CSCs. Cells were grown in a 3D sphere-formation assay to enrich for a population of cancer stem/progenitor cells. Our results showed a significant reduction in sphere formation ability. Three rounds of treatment with BLE extract were sufficient to eradicate the self-renewal ability of highly resistant CSCs. In conclusion, our results suggest a high therapeutic potential of BLE extract in targeting prostate cancer and its CSCs. PMID:25380390

El-Merahbi, Rabih; Liu, Yen-Nien; Eid, Assaad; Daoud, Georges; Hosry, Leina; Monzer, Alissar; Mouhieddine, Tarek H; Hamade, Aline; Najjar, Fadia; Abou-Kheir, Wassim

2014-01-01

95

Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats  

PubMed Central

Objective To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris (B. vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins extract on groups of rats rendered diabetic by injection of streptozotocin. Methods The phytochemical tests to detect the presence of different compounds were based on the visual observation of color change or formation of precipitate after the addition of specific reagents. Diabetes was induced in rats by intraperitoneal (i.p.) injection of streptozotocin (STZ) at a dose of 65 mg/kg bw. The fasting blood glucose levels were estimated by glucose oxidase-peroxidase reactive strips (Dextrostix, Bayer Diagnostics). Blood samples were taken by cutting the tip of the tail. Serum cholesterol and serum triglycerides were estimated by enzymatic DHBS colorimetric method. Results Administration of 62.5 and 25.0 mg/kg of saponins and aqueous extract respectively in normal rats group shows a significant hypoglycemic activity (32.33% and 40.17% respectively) during the first week. However, diabetic group treated with saponin extract produced a maximum fall of 73.1% and 76.03% at day 1 and day 21 compared to the diabetics control. Also, blood glucose levels of the diabetic rats treated with aqueous extract showed decrease of 78.79% on the first day and the effect remains roughly constant during 3 week. Both extracts also declined significantly biochemical parameters (20.77%-49.00%). The control in the loss of body weight was observed in treated diabetic rats as compared to diabetic controls. Conclusions These results demonstrated significant antidiabetic effects and showed that serum cholesterol and serum triglycerides levels were decreased, significantly, consequently this plant might be of value in diabetes treatment. PMID:23569815

Meliani, Nawel; Dib, Mohamed El Amine; Allali, Hocine; Tabti, Boufeldja

2011-01-01

96

Hepatoprotective Effects of Berberis vulgaris L. Extract/? Cyclodextrin on Carbon Tetrachloride-Induced Acute Toxicity in Mice.  

PubMed

The present study investigated the capacity of formulated Berberis vulgaris extract/?-cyclodextrin to protect liver against CCl(4)-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl(4) on the 8th day. After 24 h of CCl(4) administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl(4) was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/?-cyclodextrin treatment prevents hepatic injury induced by CCl(4) and can be considered for further nutraceutical studies. PMID:22942749

Hermenean, Anca; Popescu, Cristina; Ardelean, Aurel; Stan, Miruna; Hadaruga, Nicoleta; Mihali, Ciprian-Valentin; Costache, Marieta; Dinischiotu, Anca

2012-01-01

97

Century-old mystery of Puccinia striiformis life history solved with the identification of Berberis as an alternate host.  

PubMed

The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P. striiformis. Analyses using real-time polymerase chain reaction and DNA sequence confirmed the rust fungus as P. striiformis. Pycnia and aecia were produced on B. chinensis, B. holstii, B. koreana, and B. vulgaris after inoculation using germinating telia of P. striiformis f. sp. tritici. Wheat inoculated with aeciospores from B. chinensis resulted in uredinia, which demonstrated that Berberis spp. also serve as alternate hosts for the wheat stripe rust pathogen. The elucidation of the complete life history for P. striiformis f. sp. tritici will provide a powerful tool to rapidly advance our knowledge of the genetics of this rust fungus, and will lead to the development of improved strategies for a better control of stripe rust. PMID:20373963

Jin, Yue; Szabo, Les J; Carson, Martin

2010-05-01

98

Hepatoprotective Effects of Berberis vulgaris L. Extract/? Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice  

PubMed Central

The present study investigated the capacity of formulated Berberis vulgaris extract/?-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/?-cyclodextrin treatment prevents hepatic injury induced by CCl4 and can be considered for further nutraceutical studies. PMID:22942749

Hermenean, Anca; Popescu, Cristina; Ardelean, Aurel; Stan, Miruna; Hadaruga, Nicoleta; Mihali, Ciprian-Valentin; Costache, Marieta; Dinischiotu, Anca

2012-01-01

99

Legitimate seed dispersal by lizards in an alpine habitat: The case of Berberis empetrifolia (Berberidaceae) dispersed by Liolaemus belii (Tropiduridae)  

NASA Astrophysics Data System (ADS)

In this study we determined the effect of seed passage through Liolaemus bellii lizard digestive tracts on germination of fleshy-fruited Andean shrub species Berberis empetrifolia (Berberidaceae), and evaluated the effect of this passage on seed coat characteristics. In addition, we assessed the spatial patterns of fecal deposition by lizards onto various microhabitats available in the Andean environments of central Chile. The germination rate and the final percentage of lizard-ingested B. empetrifolia seeds was greater than control seeds. Comparing photographs and seed coat histological cuts, we suggest that the cutine wax present on seed coats from lizard-ingested seeds was probably removed by abrasion inside the lizards' digestive tract. Sixty-two percent of the lizard's feces was deposited on bare soil near rocks commonly inhabited by lizards. However, this microhabitat represents only 29% of the available ground cover at the study site. By enhancing seed germination and depositing seeds onto potential safe sites for recruitment, the lizard Liolaemus bellii is acting, at least qualitatively, as an effective disperser of Berberis empetrifolia.

Celedón-Neghme, Constanza; San Martin, Leonardo A.; Victoriano, Pedro F.; Cavieres, Lohengrin A.

2008-05-01

100

Antihyperglycemic and Antihyperlipidemic Effects of Fruit Aqueous Extract of Berberis integerrima Bge. in Streptozotocin-induced Diabetic Rats  

PubMed Central

Use of medicinal plants for attenuation of hyperglycemia and restoration of lipids disorder to normal level is clinically very important. The aim of present study was to evaluate the effects of Berberis integerrima Bge. fruit aqueous extract (BIFAE) on blood glucose and lipid profile in streptozotocin (STZ) - induced diabetic rats. The STZ-induced diabetic rats were treated by fruit aqueous extract of Berberis integerrima Bge. at doses (250 and 500 mg/Kg bw) and glibenclamide (0.6 mg/Kg bw) for 42 days by gavage. Blood glucose levels and body weights of rats were measured on weeks 0, 2, 4 and 6. Total lipid levels were determined in normal and STZ-induced diabetic rats after administration of the BIFAE and glibenclamide for 42 days. STZ-induced diabetic rats showed a significant (P<0.001) increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C) while body weight and high density lipoprotein HDL-cholesterolan (HDL-C) were significantly(P<0.001) decreased compared to normal rats. Daily administration of BIFAE did not possess the hypoglycemic and hypolipidaemic activity in STZ- diabetic rats during 6-week treatment period. Results indicate the usage of BIFAE in traditional medicine for the treatment of diabetes may need more investigation.

Ashraf, Hossein; Heidari, Reza; Nejati, Vahid

2014-01-01

101

Antimicrobial action of the leaf extract of moringa oleifera lam.  

PubMed

The ethnolic extract of the leaves of Moringa oleifera Lam. (Fam. Moringaceae) was tested for antimicrobial activities against Gram Positive - Bacillus cereus, Bacillus subtilis Staphylococcus aureus, Sarcina lutea: Gram negative - Escherichia coli and Acid fast Mycobacterium phlei. Significant antimicrobial activity of the extract was found in this study. PMID:22556699

Pal, S K; Mukherjee, P K; Saha, K; Pal, M; Saha, B P

1995-01-01

102

Molecular mapping of functional domains of the leukocyte receptor for endothelium, LAM-1  

PubMed Central

The human lymphocyte homing receptor LAM-1, like its murine counterpart MEL-14, functions as a mammalian lectin, and mediates the binding of leukocytes to specialized high endothelial cells in lymphoid organs (HEV). LAM-1 is a member of a new family of cell adhesion molecules, termed selectins or LEC-CAMs, which also includes ELAM-1 and PAD-GEM (GMP-140/CD62). To localize the regions of LAM-1 that are involved in cell adhesion, we developed chimeric selectins, in which various domains of PAD-GEM were substituted into LAM-1, and used these chimeric proteins to define the domain requirements for carbohydrate binding, and to localize the regions recognized by several mAb which inhibit the adhesion of lymphocytes to lymph node HEV. The binding of PPME or fucoidin, soluble complex carbohydrates that specifically define the lectin activity of LAM-1 and MEL-14, required only the lectin domain of LAM-1. The LAM1-1, LAM1-3, and LAM1-6 mAb each strongly inhibit the binding of lymphocytes to HEV in the in vitro frozen section assay, and defined three independent epitopes on LAM-1. Blocking of PPME or fucoidin binding by LAM1-3 indicated that this site is identical, or in close proximity, to the carbohydrate binding site, and analysis of the binding of LAM1-3 to chimeric selectins showed that the epitope detected by LAM1-3 is located within the lectin domain. Although the LAM1-6 epitope is also located in the lectin domain, LAM1-6 did not affect the binding of PPME or fucoidin. The LAM1-1 epitope was located in, or required, the EGF domain, and, importantly, binding of LAM1-1 significantly enhanced the binding of both PPME and fucoidin. These results suggest that adhesion mediated by LAM-1 may involve cooperativity between functionally and spatially distinct sites, and support previous data suggesting a role for the EGF domain of LAM-1 in lymphocyte adhesion to HEV. PMID:1712791

1991-01-01

103

UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kelsie Priest, Lahiru Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam  

E-print Network

Chandrawansa, Leon Lam, Manjot Toor, Marcel Olsthoorn, Mike Kai Hin Lam UBC Botanical Garden Redevelopment CIVLGarden Redevelopment Prepared for UBC CIVIL 445 and UBC Botanical Garden Prepared by CIVIL 445 Group Botanical Garden Redevelopment" is submitted by Group 1 in response to request by UBC Botanical Garden

104

The antihypertensive and vasodilator effects of aqueous extract from Berberis vulgaris fruit on hypertensive rats.  

PubMed

The aqueous extract from Berberis vulgaris fruit (B.V.) was tested to evaluate its antihypertensive effects on DOCA-induced hypertension in the rats. Hypertension was induced in male Sprague-Dawley rats (200-250 g) by DOCA-salt injection (20 mg/kg, twice weekly, for 5 weeks, s.c.) plus NaCl (1%) which was added to the animals' drinking water. Then 5 weeks later, the rats were anaesthetized with thiopental (30 mg/kg, i.p.) and the arterial blood pressure was measured. The mean arterial blood pressure and heart rate were 231 +/- 6.4 (mmHg) and 506 +/- 12 (beats/min), respectively. Administration of B.V. extracts significantly reduced the rat arterial blood pressure. In in vitro studies, rings of descending aorta were cut and mounted for isometric tension recording in an organ chamber containing Krebs solution. Mesenteric beds were also removed and perfused with Krebs solution. After 1 h of stabilization, preparations (aortic rings or mesenteric beds) were precontracted with phenylephrine (10(-5) M), then different concentrations of B.V. (0.4, 2 and 4 mg/mL) were added which caused a relaxation in these vessels. To investigate the mechanism of action of the extract, the tissues were incubated with either L-NAME (10(-5) M) or indomethacin (10(-5) M) for 20 min. In the aortic rings L-NAME pretreatment could only reduce the vasodilatory effects of a low concentration of B.V. (0.4 mg/mL), but indomethacin was without effect. In isolated perfused mesenteric beds preincubation with either L-NAME or indomethacin did not modify the vasodilator effects of the aqueous extract from B.V. fruit. The present results suggest that the antihypertensive and vasodilatory effects of B.V. fruit extract are mainly endothelial-independent and it may be used to treat hypertension, a status with endothelial dysfunction. PMID:15934023

Fatehi-Hassanabad, Zahra; Jafarzadeh, Mostafa; Tarhini, Ahmad; Fatehi, Mohammad

2005-03-01

105

Flüchtige Inhaltsstoffe aus Steinklee ( Melilotus officinalis L. Lam.)  

Microsoft Academic Search

Summary Using MPLC the extract obtained by solid-liquid extraction of dry sweet clover herb (Melilotus officinalis L. Lam.) with pentane\\/dichloromethane (2+1, by vol.) was fractionated. The subsequent separation and identification of the volatiles in the preseparated fractions was carried out by high resolution capillary gas chromatography (HRGC) and on-line HRGC\\/mass spectrometry (HRGC\\/MS). In total, 84 volatile constituents of sweet clover

Martin Wörner; Peter Schreier

1990-01-01

106

Immunological relatedness of the LamB proteins among members of Enterobacteriaceae.  

PubMed

We have studied the immunological relatedness of LamB proteins from a wide range of enterobacterial species, using antibodies directed against denatured Escherichia coli K12 and Klebsiella pneumoniae LamB proteins (LamBE.c. and LamBK.p., respectively), and anti-peptide antibodies directed against 10 distinct loops of LamB from E. coli K12 predicted to protrude either side of the outer membrane. We have shown that a protein immunologically related to LamBE.c. and LamBK.p. was present in all members of Enterobacteriaceae tested. A protein recognized by several anti-peptide antibodies was identified in E. coli, Shigella sonnei, Salmonella typhimurium and Kleb. pneumoniae, as well as in two Citrobacter species, two Enterobacter species and Kluyvera ascorbata. The recognition patterns obtained with the anti-peptide antibodies were in agreement with the LamB protein sequence data available. They indicated that the cell surface and also the periplasmic loops of LamB are subject to great antigenic variability. PMID:8515243

Werts, C; O'Callaghan, D; Hofnung, M; Charbit, A

1993-04-01

107

Molecular Cloning and Heterologous Expression of a cDNA Encoding Berbamunine Synthase, a CO Phenol-Coupling Cytochrome P450 from the Higher Plant Berberis stolonifera  

Microsoft Academic Search

A cDNA encoding a cytochrome P450-dependent oxidase, berbamunine synthase (EC 1.1.3.34; CYP80), from cell suspension cultures of the higher plant Berberis stolonifera Koehne and Wolf (barberry) has been isolated and heterologously expressed in functional form in insect cell culture using a baculovirus-based expression system. This cytochrome P450-dependent enzyme is unusual in that it catalyzes the regio- and stereoselective formation of

Peter F. X. Kraus; Toni M. Kutchan

1995-01-01

108

A survey of fungal plant pathogens associated with weed infestations of barberry ( Berberis spp.) in New Zealand and their biocontrol potential  

Microsoft Academic Search

Since the introduction and subsequent naturalisation of five species of Berberis into New Zealand, two species, B. glaucocarpa and B. darwinii have become aggressive invaders of both agricultural and native ecosystems throughout many regions. Both are now targets\\u000a for a biological control program. A survey for pathogens to be used as potential classical or inundative biocontrol agents\\u000a was initiated on

N. W. Waipara; L. A. Smith; A. F. Gianotti; J. P. Wilkie; C. J. Winks; E. H. C. McKenzie

2005-01-01

109

Seedling Populations Produced by Colored-leaf Genotypes of Japanese Barberry (Berberis thunbergii DC.) Contain Seedlings with Green Leaf Phenotype1  

Microsoft Academic Search

The leaf color of seedling populations derived from ornamental genotypes of Japanese barberry ( Berberis thunbergii DC.) was evaluated to determine whether nursery selections of this important landscape plant could be expected to produce green-leaf progeny or seedlings with leaf color resembling the purple-leaf or yellow-leaf parent. This is a compelling inquiry since nearly all B. thunbergii plants found within

Jonathan M. Lehrer; Mark H. Brand; Jessica D. Lubell

2006-01-01

110

Time domain modelling of room acoustics Y.W. Lam and J. Hargreaves  

E-print Network

Time domain modelling of room acoustics Y.W. Lam and J. Hargreaves University of Salford, School of Computing, Science and Engineering, M5 4WT Salford, UK y.w.lam@salford.ac.uk Proceedings of the Acoustics advantages for room acoustics investigations. It directly generates the efficient for broadband calculations

Paris-Sud XI, Université de

111

Real-time radiography support for Titan LAM  

NASA Astrophysics Data System (ADS)

This paper discusses real-time radiography (RTR) support for the Titan Lightweight Analog Motor (LAM) cold gas tests. RTR was used as a diagnostic technique to measure propellant deformation within the motors as gaseous nitrogen, at various pressures, was flowed over the propellant grain. The data consisted of video images that correlated the propellant deformation to time and to chamber pressure. Measurements were made on three propellant configurations in 17 tests. Specific issues addressed include the approach taken to gather the data, the system layout, and image processing techniques used to interpret the data.

Anderson, M. G.

1992-07-01

112

Hydrocarbon composition of crude oil from Lam Bank  

SciTech Connect

The authors discuss the crude oil from a new offshore field called the Lam Bank in the Caspian Sea. A segregated commercial crude was distilled and the distillation data is shown. In order to determine the content of n-paraffins, the naphthenic-paraffinic part of the narrow cuts was subjected to adsorptive separation on CaA zeolite. Owing to the high contents of naphthenic and isoparaffinic hydrocarbons and the low content of aromatic hydrocarbons in the distillate part, this crude can be used to produce high-quality fuels and oils by the use of the dewaxing processes.

Samedova, F.I.; Agaeva, R.M.; Alieva, F.Z.; Valiev, M.A.

1987-07-01

113

Construction and first characterization of two reciprocal hybrids between LamB from Escherichia coli K12 and Klebsiella pneumoniae.  

PubMed

The LamB proteins from Klebsiella pneumoniae and Escherichia coli K12 were previously shown to be highly homologous. The most conserved parts correspond to the N-proximal third and to the transmembranous portions of the molecule, while the variability occurred essentially within regions exposed to the cell surface or to the periplasm. Since the two proteins displayed identical in vitro trimer stability and in vivo pore properties, we tested whether the N-terminal parts of the two proteins could be exchanged and still allow the formation of stable and functional maltoporins. For that purpose, we expressed the LamB protein from K. pneumoniae in E. coli K12, and constructed two reciprocal hybrids between LamB from E. coli K12 and LamB from K. pneumoniae. The first hybrid (LamBE.c.-K.p.) is composed of residues 1 to 183 from LamBE.c. followed by residues 184 to 404 from LamBK.p. The second one comprises residues 1 to 183 from LamBK.p., followed by residues 184 to 421 from LamBE.c. (LamBK.p.-E.c.). Both hybrid proteins were correctly incorporated in the outer membrane of E. coli K12. Like the two parental LamB proteins, the two hybrids could be purified by affinity chromatography on a starch-sepharose column. The LamBE.c.-K.p. hybrid formed highly stable trimers, but was strongly impaired in its in vivo maltose transport function (15% of the wild-type level). The trimers formed by LamBK.p.-E.c. hybrid were less stable, but could be detected on the surface of intact cells by four anti-LamBE.c. monoclonal antibodies. This hybrid was also affected in its in vivo maltose transport function (30% of the wild-type level). As expected from the location of the residues critical for phage adsorption, both proteins had lost the phage receptor activity of the E. coli K12 LamB protein. We also examined whether LamBE.c. could form heterotrimers with LamBK.p., LamBK.p.-E.c., and LamBE.c.-K.p. In no case were heterotrimers detected, indicating that both terminal parts of the LamB protein are involved in homotrimer formation. All these data suggest that trimer formation and activity involve rare variable residues in the conserved regions and/or variable regions. PMID:7504315

Werts, C; Charbit, A

1993-05-01

114

Effect of root bark extract of Berberis vulgaris L. on Leishmania major on BALB/c mice.  

PubMed

Leishmaniasis is one of the most important diseases transmitted by arthropod. This disease is present in 88 countries. Approximately 400 million people are at risk, and 12 million are involved. We aimed to examine the application of ethanolic extract of the root bark of Berberis vulgaris L. for treatment of mice infected with cutaneous leishmaniasis. At first, 40 BALB/c mice were infected to Leishmania major promastigotes and were divided in two groups A and B. Then, each of A and B groups were divided to two subgroups. Mice from subgroup A1 were treated with 10% root bark alcoholic extract, and mice from subgroup A2 were treated with only alcohol (control). Mice from subgroup B1 were treated with 20% root bark alcoholic extract, and mice from subgroup B2 were treated with only alcohol (control). The 90% recovery was found in the mice treated with 20% root bark extract, and 55% recovery was found with 10% root bark extract, but in the control group, 0% recovery was found. The results of our study showed that the lotion of root bark extract has good suppression effects on parasites. Therefore, it might be a pro for developing new antileishmanial drugs. PMID:24337510

Salehabadi, Alireza; Karamian, Mahdi; Farzad, Motevalli Haghi; Namaei, Mohammad Hasan

2014-03-01

115

Comparative Study of Berberis vulgaris Fruit Extract and Berberine Chloride Effects on Acetic Acid-Induced Colitis in Rats.  

PubMed

Antioxidant and immunomodulatory effects of anthocyanins are abundant in berberry fruits suggesting that they may have beneficial effects on inflammatory bowel diseases (IBD). The present study was carried out to investigate the anti-colitic effect of Berberis vulgaris fruit extract (BFE) compared to berberine chloride (BEC) and corticosteroids using an animal model of acetic acid induced experimental colitis. BFE with three different doses (375, 750, and 1500 mg/Kg) was administered orally or rectally prior to ulcer induction. BEC (10 mg/Kg), prednisolone (5 mg/Kg), hydrocortisone acetate enema (20 mg/Kg) and normal saline (5 mL/Kg) were considered as respective controls. The tissue was assessed macroscopically for damage scores, area, index and weight/length ratio. They were also examined histopathologically for inflammation extent and severity, crypt damage, invasion involvement and total colitis index. Results indicated that greater doses of oral BFE (750, 1500 mg/Kg) as well as BEC (10 mg/Kg) were effective to protect against colonic damage. By rectal pretreatment, the extract was only effective to diminish the ulcer index and the efficacy was not significant for mucosal inflammation parameters. In conclusion BFE, which is nearly devoid of berberine, was effective to protect against colitis and this might be attributed to its anthocyanin constituents. PMID:24363687

Minaiyan, Mohsen; Ghannadi, Alireza; Mahzouni, Parvin; Jaffari-Shirazi, Elham

2011-01-01

116

Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids.  

PubMed

Extracts obtained from the roots of Berberidaceae species have been used in Eastern and Bulgarian folk medicine in rheumatic and other chronic inflammatory disorders. The investigations of the chemical composition and immunological properties show that their activity is mainly due to the alkaloid constituents. In the present study the anti-inflammatory properties of total ethanol extract (TEE), three alkaloid fractions, a major alkaloid berberine and oxyacanthine isolated from Berberis vulgaris roots were compared. All these were applied in acute inflammation (carrageenan- and zymosan-induced paw oedema), as the TEE showed the highest reducing effect. Their ability to alter in vivo and in vitro complement activity was determined. Also, the TEE was most effective in a chronic inflammatory model of adjuvant arthritis. The protoberberine fractions Bv2, Bv3 and berberine suppressed a delayed type hypersensitivity (DTH) reaction. Fraction Bv1 and berberine diminished antibody response against SRBC in vivo. The in vitro treatment of splenocytes with berberine showed that the anti-SRBC antibody synthesis was influenced in a different manner depending on the time course of its application. Oxyacanthine was less effective than berberine in the tests used. PMID:9080249

Ivanovska, N; Philipov, S

1996-10-01

117

Antioxidant Properties of Berberis aetnensis C. Presl (Berberidaceae) Roots Extract and Protective Effects on Astroglial Cell Cultures  

PubMed Central

Berberis aetnensis C. Presl (Berberidaceae) is a bushy-spiny shrub common on Mount Etna (Sicily). We demonstrated that the alkaloid extract of roots of B. aetnensis C. Presl contains prevalently berberine and berbamine, possesses antimicrobial properties, and was able to counteract the upregulation evoked by glutamate of tissue transglutaminase in primary rat astroglial cell cultures. Until now, there are no reports regarding antioxidant properties of B. aetnensis C. Presl collected in Sicily. Air-dried, powdered roots of B. aetnensis C. Presl were extracted, identified, and quantified by HPLC. We assessed in cellular free system its effect on superoxide anion, radicals scavenging activity of antioxidants against free radicals like the 1,1-diphenyl-2-picrylhydrazyl radical, and the inhibition of xanthine oxidase activity. In primary rat astroglial cell cultures, exposed to glutamate, we evaluated the effect of the extract on glutathione levels and on intracellular production of reactive oxygen species generated by glutamate. The alkaloid extract of B. aetnensis C. Presl inhibited superoxide anion, restored to control values, the decrease of GSH levels, and the production of reactive oxygen species. Potent antioxidant activities of the alkaloid extract of roots of B. aetnensis C. Presl may be one of the mechanisms by which the extract is effective against health disorders associated to oxidative stress. PMID:25177720

Campisi, Agata; Bonfanti, Roberta; Raciti, Giuseppina; Amodeo, Andrea; Mastrojeni, Silvana; Ragusa, Salvatore; Iauk, Liliana

2014-01-01

118

Antioxidant properties of Berberis aetnensis C. Presl (Berberidaceae) roots extract and protective effects on astroglial cell cultures.  

PubMed

Berberis aetnensis C. Presl (Berberidaceae) is a bushy-spiny shrub common on Mount Etna (Sicily). We demonstrated that the alkaloid extract of roots of B. aetnensis C. Presl contains prevalently berberine and berbamine, possesses antimicrobial properties, and was able to counteract the upregulation evoked by glutamate of tissue transglutaminase in primary rat astroglial cell cultures. Until now, there are no reports regarding antioxidant properties of B. aetnensis C. Presl collected in Sicily. Air-dried, powdered roots of B. aetnensis C. Presl were extracted, identified, and quantified by HPLC. We assessed in cellular free system its effect on superoxide anion, radicals scavenging activity of antioxidants against free radicals like the 1,1-diphenyl-2-picrylhydrazyl radical, and the inhibition of xanthine oxidase activity. In primary rat astroglial cell cultures, exposed to glutamate, we evaluated the effect of the extract on glutathione levels and on intracellular production of reactive oxygen species generated by glutamate. The alkaloid extract of B. aetnensis C. Presl inhibited superoxide anion, restored to control values, the decrease of GSH levels, and the production of reactive oxygen species. Potent antioxidant activities of the alkaloid extract of roots of B. aetnensis C. Presl may be one of the mechanisms by which the extract is effective against health disorders associated to oxidative stress. PMID:25177720

Campisi, Agata; Acquaviva, Rosaria; Bonfanti, Roberta; Raciti, Giuseppina; Amodeo, Andrea; Mastrojeni, Silvana; Ragusa, Salvatore; Iauk, Liliana

2014-01-01

119

Expression of the human leukocyte adhesion molecule, LAM1. Identity with the TQ1 and Leu-8 differentiation antigens.  

PubMed

The LAM1 molecule is a member of the new family of cellular adhesion/homing molecules that contain a lectin-like domain at their amino-terminal end followed by an epidermal growth factor-like domain and short consensus repeat units like those found in C3/C4 binding proteins. Two mAb that react with the leukocyte adhesion molecule 1 (LAM1) were produced and used to examine the cell-surface expression of LAM1. The anti-LAM1 antibodies were reactive with the majority of blood lymphocytes, NK cells, neutrophils, and monocytes. LAM1 was also expressed by subpopulations of phenotypically immature and mature thymocytes. Blood lymphocytes rapidly modulated LAM1 from the cell surface during PMA exposure for 60 min. Coordinate with the loss of LAM1 from the cell surface, PMA-treated lymphocytes lost the ability to bind to lymph node high endothelial venules, indicating that expression of LAM1 may play a role in lymphocyte homing. Mitogen stimulation of blood T and B lymphocytes also resulted in decreased LAM1 expression, but at a slower rate. LAM1 was only weakly expressed by a minority of spleen lymphocytes. However, culturing spleen lymphocytes in media alone resulted in increased expression of LAM1 by a subpopulation of the cells (40 to 60%). Concomitant mitogen stimulation of spleen lymphocytes resulted initially in down-regulation of LAM1 expression followed by increased expression of LAM1 and then subsequent loss of LAM1 from the cell surface. The pattern of anti-LAM1 antibody reactivity was identical to that reported for the TQ1 and Leu-8 antibodies, and all of these antibodies reacted with cells transfected with the LAM1 cDNA. Thus, LAM1 is broadly expressed by leukocytes, and binding of LAM1 may participate in the process of leukocyte extravasation into lymphoid organs or sites of acute inflammation with subsequent loss of LAM1 from the cell surface. PMID:1688580

Tedder, T F; Penta, A C; Levine, H B; Freedman, A S

1990-01-15

120

Therapeutic options for lymphangioleiomyomatosis (LAM): where we are and where we are going  

PubMed Central

Lymphangioleiomyomatosis (LAM), a multisystem disease affecting predominantly premenopausal and middle-aged women, causes progressive respiratory failure due to cystic lung destruction and is associated with lymphatic and kidney tumors. In the past, the treatment of LAM comprised exclusively anti-estrogen and related hormonal therapies. These treatments, however, have not been proven effective. In this article, we discuss new findings regarding the molecular mechanisms involved in the regulation of LAM cell growth, which may offer opportunities to develop effective and targeted therapeutic agents. PMID:20948684

Steagall, Wendy K; Moss, Joel

2009-01-01

121

Phytochemical Investigations on Chemical Constituents of Achillea tenuifolia Lam.  

PubMed

Achillea tenuifolia Lam. (Asteraceae) afforded a methanolic extract from which after fractionation in solvents with different polarities, two known flavones 3', 5- dihydroxy- 4', 6, 7- trimethoxy flavone (eupatorine, compound 3), 5- hydroxy- 3',4', 6, 7- tetramethoxyflavone (compound 4), besides stearic acid (compound 1), lupeol (compound 2), daucosterol (?- sitosterol 3-O- ?- D- glucopyranoside, compound 5), 2, 4- dihydroxy methyl benzoate (compound 6) were isolated for the first time. The structure of isolated compounds was elucidated by means of different spectroscopic methods such as UV, IR, Mass and 1H- NMR (1D and 2D) and 13C-NMR. For further confirming the structures of isolated compounds, comparison of the spectral data of them with those reported in the litratures have been done. PMID:25276207

Moradkhani, Shirin; Kobarfard, Farzad; Ayatollahi, Seyed Abdol Majid

2014-01-01

122

Ipomoea batatas (L.) Lam.: a rich source of lipophilic phytochemicals.  

PubMed

The lipophilic extracts from the storage root of 13 cultivars of sweet potato (Ipomoea batatas (L.) Lam.) were evaluated by gas chromatography-mass spectrometry with the aim to valorize them and offer information on their nutritional properties and potential health benefits. The amount of lipophilic extractives ranged from 0.87 to 1.32% dry weight. Fatty acids and sterols were the major families of compounds identified. The most abundant saturated and unsaturated fatty acids were hexadecanoic acid (182-428 mg/kg) and octadeca-9,12-dienoic acid (133-554 mg/kg), respectively. ?-Sitosterol was the principal phytosterol, representing 55.2-77.6% of this family, followed by campesterol. Long-chain aliphatic alcohols and ?-tocopherol were also detected but in smaller amounts. The results suggest that sweet potato should be considered as an important dietary source of lipophilic phytochemicals. PMID:24345069

Cordeiro, Nereida; Freitas, Nélia; Faria, Marisa; Gouveia, Manuela

2013-12-18

123

Phytochemical Investigations on Chemical Constituents of Achillea tenuifolia Lam  

PubMed Central

Achillea tenuifolia Lam. (Asteraceae) afforded a methanolic extract from which after fractionation in solvents with different polarities, two known flavones 3’, 5- dihydroxy- 4’, 6, 7- trimethoxy flavone (eupatorine, compound 3), 5- hydroxy- 3’,4’, 6, 7- tetramethoxyflavone (compound 4), besides stearic acid (compound 1), lupeol (compound 2), daucosterol (?- sitosterol 3-O- ?- D- glucopyranoside, compound 5), 2, 4- dihydroxy methyl benzoate (compound 6) were isolated for the first time. The structure of isolated compounds was elucidated by means of different spectroscopic methods such as UV, IR, Mass and 1H- NMR (1D and 2D) and 13C-NMR. For further confirming the structures of isolated compounds, comparison of the spectral data of them with those reported in the litratures have been done. PMID:25276207

Moradkhani, Shirin; Kobarfard, Farzad; Ayatollahi, Seyed Abdol Majid

2014-01-01

124

A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM).  

PubMed

Lymphangioleiomyomatosis (LAM) is a rare and progressive cystic lung condition affecting approximately 3.4-7.5/million women, with an average lag time between symptom onset and diagnosis of upwards of 4 years. The aim of this work was to identify altered proteins in LAM serum which may be potential biomarkers of disease. Serum from LAM patient volunteers and healthy control volunteers were pooled and analysis carried out using quantitative 4-plex iTRAQ technology. Differentially expressed proteins were validated using ELISAs and pathway analysis was carried out using Ingenuity Pathway Analysis. Fourteen proteins were differentially expressed in LAM serum compared to control serum (p<0.05). Further screening validated the observed differences in extracellular matrix remodelling proteins including fibronectin (30% decrease in LAM, p?=?0.03), von Willebrand Factor (40% reduction in LAM, p?=?0.03) and Kallikrein III (25% increase in LAM, p?=?0.03). Pathway networks elucidated the relationships between the ECM and cell trafficking in LAM. This study was the first to highlight an imbalance in networks important for remodelling in LAM, providing a set of novel potential biomarkers. These understandings may lead to a new effective treatment for LAM in the future. PMID:25133674

Banville, Nessa; Burgess, Janette K; Jaffar, Jade; Tjin, Gavin; Richeldi, Luca; Cerri, Stefania; Persiani, Elisa; Black, Judith L; Oliver, Brian G

2014-01-01

125

Analysis of Lymphangioleiomyomatosis (LAM) patent tomograms: Stereology and modeling  

SciTech Connect

Lymphangioleiomyomatosis (LAM) is an insidious disease of young women which attacks the lungs and if untreated results in death. Treatment is radical. Currently a woman diagnosed as having LAM (usually by computed tomography (CT) of the lung) is run through an exhausting set of tests and exercises to determine the extent of the disease. Image processing techniques have been able to quantify the tomograms by providing a histogram of the number of cyst intercepts of a given area. The images are digital, so the data are discrete, and the areas are in terms of square pixels. It is hoped that by analyzing the data and by recovering the probability density of the cysts themselves one can provide a set of parameters that will aid in the early diagnosis of the disease and that will correlate well with the physiology. The desire to invert the data, that is to determine the probability density of the cysts from the probability density of the circular intersections of the cysts with the tomographic plane, led to a re-examination of the Wicksell equation. In Section 2, the authors transform Wicksell`s equation in diameter (a generalized Abel equation) into an equation in areas (the original Abel equation). In Section 3 they present the adjoint equation in several equivalent forms. Analytic solutions of the adjoint equation necessary for later use are developed in Section 4. Outside of the framework of integral equations they have done some modeling of the data. They find that, to a very high degree of reliability, the data follows a simple power law. The distribution of spheres likewise follows a simple power law. These considerations are given in Section 5. Then in Section 6 they explore the exponential model as an alternative. Section 7 solves the Wicksell equation and uses the Golberg device, via the adjoint, to solve a related problem. They finally get to the number of cysts and their average size in Section 8. Their conclusions are in Section 9.

Faber, V.; Wing, G.M.; Winter, L.; Zahrt, J.D. [Los Alamos National Lab., NM (United States). Computing, Information, and Communications Div.

1995-02-01

126

[Identification of a confused species of Euphorbia hirta L. E. indica Lam].  

PubMed

The article reported the morphological and histological identification for Euporbia hirta L. and its confused species E. indica Lam.. It provided evidences for identifying Euphorbia hirta L.. PMID:11341025

Chu, X; Cao, L; Yuan, C

2001-01-01

127

Antigenic polymorphism of the LamB protein among members of the family Enterobacteriaceae.  

PubMed Central

In this study we demonstrate that most members of the family Enterobacteriaceae possess a maltose-inducible outer membrane protein homologous to the LamB protein of Escherichia coli K-12. These proteins react with polyclonal antibodies raised against the LamB protein of E. coli K-12. We compared the antigenic structure of the LamB protein in members of the family Enterobacteriaceae with six monoclonal antibodies raised against the LamB protein of E. coli K-12. Four of them reacted with epitopes located at the outer face of the membrane, and two reacted with epitopes located at the inner face of the membrane. A great degree of variability was observed for the external epitopes. Even in a single species, such as E. coli, an important polymorphism was present. In contrast, the internal epitopes were more conserved. Images PMID:4040134

Bloch, M A; Desaymard, C

1985-01-01

128

Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats  

Microsoft Academic Search

This study was carried out to evaluate the effect of Moringa oleifera Lam (Moringa) seed extract on liver fibrosis. Liver fibrosis was induced by the oral administration of 20% carbon tetrachloride (CCl4), twice weekly and for 8weeks. Simultaneously, M.oleifera Lam seed extract (1g\\/kg) was orally administered daily. The biochemical and histological results showed that Moringa reduced liver damage as well

Alaaeldin A. Hamza

2010-01-01

129

Berberis vulgaris root extract alleviates the adverse effects of heat stress via modulating hepatic nuclear transcription factors in quails.  

PubMed

To evaluate the action mode of Berberis vulgaris root extract in the alleviation of oxidative stress, female Japanese quails (n 180, aged 5 weeks) were reared, either at 22°C for 24 h/d (thermoneutral, TN) or 34°C for 8 h/d (heat stress, HS), and fed one of three diets: diets containing 0, 100 or 200 mg of B. vulgaris root extract per kg for 12 weeks. Exposure to HS depressed feed intake by 8·5% and egg production by 12·1%, increased hepatic malondialdehyde (MDA) level by 98·0% and decreased hepatic superoxide dismutase, catalase and glutathione peroxidase activities by 23·5, 35·4 and 55·7%, respectively (P<0·001 for all). There were also aggravations in expressions of hepatic NF-?B and heat-shock protein 70 (HSP70) by 42 and 43%, respectively and suppressions in expressions of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and haeme-oxygenase 1 (HO-1) by 57 and 61%, respectively, in heat-stressed quails (P<0·001 for all). As supplemental B. vulgaris extract increased, there were linear increases in performance parameters, activities of antioxidant enzymes and hepatic Nrf2 and HO-1 expressions (P<0·001 for all) and linear decreases in hepatic MDA level and NF-?B and HSP70 expressions at a greater extent in quails reared under TN condition and those reared under HS condition. In conclusion, dietary supplementation of B. vulgaris root extract to quails reduces the detrimental effects of oxidative stress and lipid peroxidation resulting from HS via activating the host defence system at the cellular level. PMID:23312115

Sahin, Kazim; Orhan, Cemal; Tuzcu, Mehmet; Borawska, Maria H; Jab?onski, Jakub; Guler, Osman; Sahin, Nurhan; Hayirli, Armagan

2013-08-01

130

[Relationships between grazing-path and Berberis aggregate population characteristics in upper reaches of Minjiang River, Southwest China].  

PubMed

Taking the Berberis aggregate shrubs in the ecotone of dry valley and montane forests in upper reaches of Minjiang River as study objects, and by the methods of tracking grazing and field survey, this paper studied the characteristics of habitat utilization by livestock and the features of grazing-path. The major factors affecting the features of grazing-path were screened by redundancy analysis (RDA), and the relationships of the grazing-path features with the coverage, size class, and distribution pattern of the shrubs were analyzed. It was shown that the distribution pattern of the grazing-path could intuitively reflect the characteristics of the habitat utilization by livestock, being in accordance with the results of tracking grazing. The Morisita index at 5 m scale could objectively reflect the distribution type of the grazing-path. Sample plots 1, 2 and 6 presented a contagious distribution of grazing-path, while the other plots showed regular distribution. In slope scale, the coverage and height of the shrubs were the notable affecting factors, which had negative correlations with the grazing-path features. There was a significant negative correlation between the coverage of B. aggregate population and the area of grazing-path. The population structure of B. aggregate had a close correlation with the distribution of grazing-path. The ratio of the long axis to short axis of the shrubs was averagely 1.29, and the shape of the shrubs approached to round. It was considered that the grazing-path landscape and the livestock on the grazing-paths had the function of reconstructing the shape of the shrubs. The directionality of the population pattern of B. aggregate was generally in line with the distribution type of grazing-path, but actually, they were opposite in distribution. The patches of the shrubs were in aggregated or uniform distribution in the areas deviated from the grazing-path. PMID:23717991

Liu, Jin-Xin; Gong, Yuan-Bo; Zheng, Jiang-Kun; Zhang, Xing-Hua; Jiang, Guang-Zheng; Yue, Yan-Jie; Zuo, Qin; Liu, Mu

2013-01-01

131

Mycobacterium tuberculosis ManLAM inhibits T-cell-receptor signaling by interference with ZAP-70, Lck and LAT phosphorylation  

PubMed Central

Immune evasion is required for Mycobacterium tuberculosis to survive in the face of robust CD4+ T cell responses. We have shown previously that M. tuberculosis cell wall glycolipids, including mannose capped lipoarabinomannan (ManLAM), directly inhibit polyclonal murine CD4+ T cell activation by blocking ZAP-70 phosphorylation. We extended these studies to antigen-specific murine CD4+ T cells and primary human T cells and found that ManLAM inhibited them as well. Lck and LAT phosphorylation also were inhibited by ManLAM without affecting their localization to lipid rafts. Inhibition of proximal TCR signaling was temperature sensitive, suggesting that ManLAM insertion into T cell membranes was required. Thus, M. tuberculosis ManLAM inhibits antigen-specific CD4+ T cell activation by interfering with very early events in TCR signaling through ManLAM's insertion in T cell membranes. PMID:22507872

Mahon, Robert N.; Sande, Obondo J.; Rojas, Roxana E.; Levine, Alan D.; Harding, Clifford V.; Boom, W. Henry

2012-01-01

132

Benzylcarbamothioethionate from root bark of Moringa oleifera Lam. and its toxicological evaluation (Bencilcarbamotioetionato de la raíz de Moringa oleifera Lam. y su evaluación toxicológica)  

Microsoft Academic Search

A new compound, benzylcarbamothioethionate (1), was isolated from the chloroform soluble fraction of the ethanolic extract of the root bark of Moringa oleifera Lam. Its structure was established on the basis of 1D and 2D-NMR and mass spectroscopy. The acute toxicity studies of chloroform extract and compound 1 were performed on Long Evan's rats using four groups (two controls and

Farjana NIKKON; Sohel HASAN; Kazi Abdus SALAM; Mohammed A. MOSADDIK; Proma KHONDKAR; Mohammed Ekramul HAQUE; Mukhlesur RAHMAN

133

New phytoconstituents from the aerial parts of Fumaria parviflora Lam  

PubMed Central

Fumaria parviflora Lam. (Fumariaceae) is an annual herb found throughout the world. Traditionally it has great significance in various disorders. In folk medicine of Turkey it is used against hepato-biliary dysfunction and imported from Iran. In Charaka and Sushruta, it is recommended for treatment of fevers, blood disorders, chronic skin diseases, urinary diseases and cough. The compounds were isolated from methanolic extract of the plants by column chromatography using silica gel (60-120 mesh) as stationary phase and structure of the isolated compounds have been established on the basis of spectral data analysis and chemical reactions. Phytochemical investigation of its aerial parts led to the isolation of five new compounds characterized as (5?H,11?H)-8-oxo-homoiridolide (1), n-docosanyl-2-O-?-D-glucopyranosyl salicylate (2), 2-methyl-6-hydroxymethylenedodecan-10-oyl-12, 15-olide14-O-?-D-xylopyranoside (3), 4-oxo-stigmast-5-en-3?-ol-D-glucopyranoside (4) and salicylic acid-O-?-D-xylopyranoside (5) along with the known compounds ?-D-glucopyranosyl hexadecanoate (6) and ?-D-glucopyranosyl- (2 ? 1?)-?-D-glucopyranoside (7). The isolated compounds are useful as they will provide essential data and information for the further researchers and development of effective analytical marker for identity, purity and quality control of this traditional plant in future. PMID:24959414

Jameel, Mohammad; Ali, Abuzer; Ali, Mohammed

2014-01-01

134

In vitro biological assessment of berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects  

PubMed Central

Background Berberis vulgaris is a well known plant with traditional herbal medical history. The aims of this study was to bioscreen and compare the in vitro biological activity (antioxidant, cholinergic, antidaibetic and the anticancer) of barberry crude extract and berberine active compound. Methods The effect of B. vulgaris extract and berberine chloride on cellular thiobarbituric acid reactive species (TBARS) formation, diphenyle–?-picrylhydrazyl (DPPH) oxidation, cellular nitric oxide (NO) radical scavenging capability, superoxide dismutase (SOD), glutathione peroxidase (GPx), acetylcholinesterase (AChE) and ?-gulcosidase activities were spectrophotometrically determined. On the other hand, the effect of extract and berberine as anticancer was estimated on three different cell lines which were MCF-7, HepG-2, and Caco-2 cells by using neutral red uptake assay which compared with control normal cells (PBMC). Results Our results showed that barberry crude extract contains 0.6 mg berberine/mg crude extract. Barberry extract showed potent antioxidative capacity through decreasing TBARS, NO and the oxidation of DPPH that associated with GPx and SOD hyperactivation. Inhibitory effect of berberis crude extract on ?-glucosidase was more potent than that of berberine chloride, while both had the same AChE inhibitory effect. Besides, different concentrations of both berberine chloride and barberry ethanolic extract showed to have no growth inhibitory effect on normal blood cells (PBMC). Otherwise, both berberine chloride and barberry ethanolic extract showed to have inhibitory effect on the growth of breast, liver and colon cancer cell lines (MCF7, HepG2 and CACO-2, respectively) at different incubation times starting from 24 hrs up to 72 hrs and the inhibitory effect increased with time in a dose dependant manner. Conclusion This work demonstrates the potential of the barberry crude extract and its active alkaloid, berberine, on suppressing lipid peroxidation, suggesting a promising use in the treatment of hepatic oxidative stress, Alzheimer and idiopathic male factor infertility. Beside, berberis vulgaris ethanolic extract is safe non-toxic extract as it was not inhibit the growth of PBMC that can induce cancer cell death that could return to its powerful antioxidant activity. PMID:24007270

2013-01-01

135

A Sacrificial Lam: A Divided School Board, a Beleaguered Superintendent, and an Urgent Need to Improve Student Achievement  

ERIC Educational Resources Information Center

This case describes the confrontational relationship between four trustees on the San Antonio School Board and the San Antonio School District's superintendent Diana Lam, a nationally recognized school reformer, who came to San Antonio in 1994. The case includes a dramatic board meeting where a closely divided board meets to buy out Lam's…

Fossey, Richard

2011-01-01

136

www.mdpi.com/journal/ijms Hepatoprotective Effects of Berberis vulgaris L. Extract/? Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice  

E-print Network

Abstract: The present study investigated the capacity of formulated Berberis vulgaris extract/?-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Int. J. Mol. Sci. 2012, 13 9015 Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT,

Anca Hermenean; Cristina Popescu; Aurel Ardelean; Miruna Stan; Nicoleta Hadaruga; Ciprian-valentin Mihali; Marieta Costache; Anca Dinischiotu

2012-01-01

137

Leaf phenology and seasonal variation of photosynthesis of invasive Berberis thunbergii (Japanese barberry) and two co-occurring native understory shrubs in a northeastern United States deciduous forest.  

PubMed

Early leafing and extended leaf longevity can be important mechanisms for the invasion of the forest understory. We compared the leaf phenology and photosynthetic characteristics of Berberis thunbergii, an early leafing invasive shrub, and two co-occurring native species, evergreen Kalmia latifolia and late leafing Vaccinium corymbosum, throughout the 2004 growing season. Berberis thunbergii leafed out 1 month earlier than V. corymbosum and approximately 2 weeks prior to the overstory trees. The photosynthetic capacity [characterized by the maximum carboxylation rate of Rubisco (V (cmax)) and the RuBP regeneration capacity mediated by the maximum electron transport rate (J (max))] of B. thunbergii was highest in the spring open canopy, and declined with canopy closure. The 2003 overwintering leaves of K. latifolia displayed high V (cmax) and J (max) in spring 2004. In new leaves of K. latifolia produced in 2004, the photosynthetic capacity gradually increased to a peak in mid-September, and reduced in late November. V. corymbosum, by contrast, maintained low V (cmax) and J (max) throughout the growing season. In B. thunbergii, light acclimation was mediated by adjustment in both leaf mass per unit area and leaf N on a mass basis, but this adjustment was weaker or absent in K. latifolia and V. corymbosum. These results indicated that B. thunbergii utilized high irradiance in the spring while K. latifolia took advantage of high irradiance in the fall and the following spring. By contrast, V. corymbosum generally did not experience a high irradiance environment and was adapted to the low irradiance understory. The apparent success of B. thunbergii therefore, appeared related to a high spring C subsidy and subsequent acclimation to varying irradiance through active N reallocation and leaf morphological modifications. PMID:17647021

Xu, Cheng-Yuan; Griffin, Kevin L; Schuster, W S F

2007-11-01

138

Jackfruit (Artocarpus heterophyllus Lam.) Diversity in Bangladesh: Land Use and Artificial Selection1  

E-print Network

Jackfruit (Artocarpus heterophyllus Lam.) Diversity in Bangladesh: Land Use and Artificial in Bangladesh: Land Use and Artificial Selection. Bangladesh is often recognized as a secondary center diversity across trees associated with a gradient of three locations/habitats (homesteads, public lands

Fant, Jeremie

139

Effect of Moringa oleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats  

Microsoft Academic Search

In India, drumstick (Moringa oleifera Lam. (Moringaceae)) is commonly used as a phytotherapeutic agent. The effect of oral administration of aqueous and alcoholic extract of Moringa oleifera root-wood on calcium oxalate urolithiasis has been studied in male Wistar albino rats. Ethylene glycol feeding resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. Supplementation with aqueous and

Ravindra V. Karadi; Navneet B. Gadge; K. R. Alagawadi; Rudraprabhu V. Savadi

2006-01-01

140

NASA FOIA PUBLIC LIAISON OFFICERS and REQUESTER SERVICE CENTERS Miriam Brown-Lam  

E-print Network

NASA FOIA PUBLIC LIAISON OFFICERS and REQUESTER SERVICE CENTERS Miriam Brown-Lam Principal Agency FOIA Officer Chief, NASA FOIA Public Liaison Officer (202) 358-0718 Josephine Sibley HQ FOIA Public Research Center (ARC) Lubna Shirzai ARC FOIA Public Liaison Officer NASA Ames Research Center Mail Stop 943

Waliser, Duane E.

141

ROME: Routing On Metropolitan-scale Ethernet Chen Qian and Simon S. Lam  

E-print Network

ROME: Routing On Metropolitan-scale Ethernet Chen Qian and Simon S. Lam Department of Computer Ethernet [1], [3], [9], [10], wide area Ethernet [4], data center networks [8], as well as enterprise-scale Ethernet. Ethernet offers plug-and-play functionality and a flat MAC address space. Ethernet MAC addresses

Hayes, Jane E.

142

Reflection Analysis for Java Benjamin Livshits, John Whaley, and Monica S. Lam  

E-print Network

Reflection Analysis for Java Benjamin Livshits, John Whaley, and Monica S. Lam Computer Science. Reflection has always been a thorn in the side of Java static analysis tools. Without a full treatment of reflection, static analysis tools are both incomplete because some parts of the program may not be in- cluded

Livshits, Ben

143

J'ecoute votre voix Lam khone Savane, COUf d'amouf lao  

E-print Network

soirees entre amis. La cour d'amour, lam kiao, en est sans doute la forme la plus accomplie, celIe qui'altemance des interpretes. Poesie enfin, car la cour d'amour est un instant privilegie, ou s'affrontent les vie quotidienne et les mysteres de I'amour. Dan

Paris-Sud XI, Université de

144

Antifungal activity of crude extracts and essential oil of Moringa oleifera Lam  

Microsoft Academic Search

Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medicines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum, and Microsporum canis. GC–MS analysis of the chemical composition of the essential oil from leaves showed a total of 44 compounds. Isolated

Ping-Hsien Chuang; Chi-Wei Lee; Jia-Ying Chou; M. Murugan; Bor-Jinn Shieh; Hueih-Min Chen

2007-01-01

145

Short Communication Antifungal activity of crude extracts and essential oil of Moringa oleifera Lam  

Microsoft Academic Search

Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medi- cines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagro- phytes, Epidermophyton Xoccosum, and Microsporum canis. GC-MS analysis of the chemical composition of the essential oil from leaves showed a total of 44

Ping-Hsien Chuang; Chi-Wei Lee; Jia-Ying Chou; M. Murugan; Bor-Jinn Shieh; Hueih-Min Chen

146

Removal of cadmium from aqueous system by shelled Moringa oleifera Lam. seed powder  

Microsoft Academic Search

The present study explores the unexploited sorption properties of the plant Moringa oleifera Lam. for decontamination of Cd at laboratory scale. Sorption studies using standard practices were carried out in batch experiments as functions of biomass dosage, contact time, metal concentrations, particle size and pH. Percentage sorption in each case was computed on the basis of Cd estimation using a

Parul Sharma; Pushpa Kumari; M. M. Srivastava; Shalini Srivastava

2006-01-01

147

RFID Paperclip Tags Pavel V. Nikitin, K. V. S. Rao, and Sander Lam  

E-print Network

RFID Paperclip Tags Pavel V. Nikitin, K. V. S. Rao, and Sander Lam Intermec Technologies.com Abstract-- In this paper, we present RFID tags which double as paperclips. These tags use standard metal RFID functionality. Paperclip tags can be designed so that as they slide on or off the stack of papers

Hochberg, Michael

148

Chapter 7.13 Lam8 Poly-Si TCP Etcher  

E-print Network

single crystal silicon, polycrystalline silicon, silicon carbide, silicon germanium, and germanium films, cassette-to-cassette, TCP etcher for Poly-Silicon etching. This tool is currently dedicated to etching be programmed to end the etch process at a specified condition. Lam8 uses Channel A (405 nm for poly-silicon

Healy, Kevin Edward

149

SQUASH BEETLE ON CUCURBITS Frankie Lam, Ricky E. Foster, Extension Entomologists,  

E-print Network

SQUASH BEETLE ON CUCURBITS Frankie Lam, Ricky E. Foster, Extension Entomologists, Dan Egel to the family of lady beetles (Coccinellidae). Most insects in this family are beneficial, often eating large numbers of pest insects such as aphids. The exceptions are the squash beetle and the Mexican bean beetle

Ginzel, Matthew

150

Feeding preferences of the collembolan Onychiurus sinensis for fungi colonizing holm oak litter (Quercus rotundifolia Lam.)  

E-print Network

1 Feeding preferences of the collembolan Onychiurus sinensis for fungi colonizing holm oak litter-francois.ponge@wanadoo.fr) Abstract - Ten fungi isolated from decaying holm oak leaves (Quercus rotundifolia Lam.) have been presented to the collembolan species Onychiurus sinensis (Hexapoda). The attractiveness and selectivity of the ten fungi

Paris-Sud XI, Université de

151

Catching Old Influenza Virus with A New Markov Model HamChing Lam  

E-print Network

Catching Old Influenza Virus with A New Markov Model HamChing Lam Dept. of Computer Science on the Hemag- glutinin (HA) gene, a major surface antigen of the avian influenza virus. Using this model we Influenza virus, Poisson process, Markov Model 1. INTRODUCTION For the past century researchers have been

Boley, Daniel

152

Analyze Influenza Virus Sequences Using Binary Encoding HamChing Lam  

E-print Network

Analyze Influenza Virus Sequences Using Binary Encoding Approach HamChing Lam Dept. of Computer 55455, USA boley@cs.umn.edu ABSTRACT Capturing mutation patterns of each individual influenza virus distance. We first convert the influenza genetic sequence to a binary string and then ap- ply Principal

Boley, Daniel

153

Predicting weed migration from soil and climate maps. [Centaurea maculosa Lam  

Microsoft Academic Search

Soil characteristics, elevation, annual precipitation, potential evapotranspiration, length of frost-free season, and mean maximum July temperature were estimated for 116 established infestations of spotted knapweed (Centaurea maculosa Lam. number³ CENMA) in Montana using basic land resource maps. Areas potentially vulnerable to invasion by the plant were delineated on the basis of representative edaphic and climatic characteristics. No single environmental variable

T. K. Chicoine; P. K. Fay; G. A. Nielsen

1985-01-01

154

Bionic lightweight design by laser additive manufacturing (LAM) for aircraft industry  

Microsoft Academic Search

Today many challenges lie ahead of the aircraft industry. The increasing competition and shortage of resources raise a challenge for future manufacturing technologies and lightweight design. A possibility to cope with these circumstances is the manufacturing technology of Laser Additive Manufacturing (LAM). However there are still challenges to cope with due to the processes novelty, such as the development of

Claus Emmelmann; Maren Petersen; Jannis Kranz; Eric Wycisk

2011-01-01

155

FUNGAL COLONIZATION OF PHYLLOSPHERE AND LITTER OF QUERCUS ROTUNDIFOLIA LAM. IN A HOLM OAK FOREST (HIGH  

E-print Network

FUNGAL COLONIZATION OF PHYLLOSPHERE AND LITTER OF QUERCUS ROTUNDIFOLIA LAM. IN A HOLM OAK FOREST-36" DOI : 10.1007/s00374-003-0666-6 #12;2 SUMMARY: The microfungal flora of holm oak living, senesced methods. Holm oak leaves are first colonized on the tree by a variety of primary saprophytes

Boyer, Edmond

156

Polysaccharides of Berberis vulgaris  

Microsoft Academic Search

The inflorescence were collected in the period of mass flowering (May 31), and fruit and leaves in the phase of technical ripening of the fruit (September 28) in the environs of Ryazan' in 1981. The polysaccharides (PSs) from the air-dry raw material (moisture content of the flowers and leaves 9.2-10.2%, and of the fruit 10.1-11.0%) that had been twice extracted

E. G. Martynov; E. A. Stroev; D. D. Peskov

1984-01-01

157

A model of maltodextrin transport through the sugar-specific porin, LamB, based on deletion analysis.  

PubMed

LamB facilitates the uptake of maltose and maltodextrins across the bacterial outer membrane and acts as a general porin for small molecules. Using directed deletion mutagenesis we removed several regions of the LamB polypeptide and identified a polypeptide loop that both constricts the maltoporin channel and binds maltodextrins. In conjunction with a second sugar binding site that we identified at the rim of the channel, these data clarify, for the first time, the mechanism of transport through a substrate-specific porin. Furthermore, unlike the transverse loops of general porins, which originate from a central location in their primary structure, the loop that regulates LamB permeability originates from a C-terminal site. Thus LamB represents a second distinct class of porins in the bacterial outer membrane that is differently organized and separately evolved from OmpF-type, general porins. PMID:7925308

Klebba, P E; Hofnung, M; Charbit, A

1994-10-01

158

A model of maltodextrin transport through the sugar-specific porin, LamB, based on deletion analysis.  

PubMed Central

LamB facilitates the uptake of maltose and maltodextrins across the bacterial outer membrane and acts as a general porin for small molecules. Using directed deletion mutagenesis we removed several regions of the LamB polypeptide and identified a polypeptide loop that both constricts the maltoporin channel and binds maltodextrins. In conjunction with a second sugar binding site that we identified at the rim of the channel, these data clarify, for the first time, the mechanism of transport through a substrate-specific porin. Furthermore, unlike the transverse loops of general porins, which originate from a central location in their primary structure, the loop that regulates LamB permeability originates from a C-terminal site. Thus LamB represents a second distinct class of porins in the bacterial outer membrane that is differently organized and separately evolved from OmpF-type, general porins. Images PMID:7925308

Klebba, P E; Hofnung, M; Charbit, A

1994-01-01

159

Inhibitory Effect of Berberis vulgaris Aqueous Extract on Acquisition and Reinstatement Effects of Morphine in Conditioned Place Preferences (CPP) in Mice  

PubMed Central

Background: It has been elucidated that Berberis vulgaris (barberry) can alleviate morphine withdrawal syndrome. Also it has been reported that aqueous extract of barberry possibly have inhibitory effect on NMDA receptors. Objectives: In this study, we decided to evaluate the effects of aqueous extract of B. vulgaris fruit on morphine tendency in mice using conditioned place preference (CPP) method. Materials and Methods: In experiment 1 (acquisition phase), mice underwent morphine-induced conditioned place preference (CPP) training with injections of morphine (40 mg/kg). In experiment 2 (extinction and reinstatement phases), mice underwent the same CPP training as in experiment 1 and subsequent extinction training on day 16th a reinstatement by CPP was done by injection of reminding 10 mg/kg morphine. Results: The administration of morphine (40 mg/kg for four days) produced place preference. In the first method, the aqueous extract of barberry (200 mg/kg) prevented morphine tendency to white cell in CPP method. In the second method, after inter-peritoneal injection of aqueous extracts of barberry at 100 and 200 mg/kg, the animals tendency toward the white cells of CPP chamber on the sixteenth day (after a reminder injection of morphine 10 mg/kg) was significantly reduced. Conclusions: These results show that aqueous extract of barberry can reduce the acquisition and reinstatement of morphine-induced conditioned place preference. PMID:25237645

Imenshahidi, Mohsen; Qaredashi, Reza; Hashemzaei, Mahmoud; Hosseinzadeh, Hossein

2014-01-01

160

High fat diet induced insulin resistance and elevated retinol binding protein 4 in female rats; treatment and protection with Berberis vulgaris extract and vitamin A.  

PubMed

This research was conducted to investigate two main aims; the first aim was to find if there is a relationship between insulin resistance (IR) and retinol binding protein 4 (RBP4). The second aim was to use berberis vulgaris extract and vitamin A as protective and/or curative agents against insulin resistance. IR was developed by feeding the female rats a high fat diet (HFD) for six weeks then treating or protecting them with b. vulgaris extract (0.2 g/Kg body weight) or vitamin A (12.8?g/Kg/day) for two weeks. HFD intake elevated insulin level and RBP4 expression that associated with hyperglycemia and hyperlipidemia. Co-administration of vitamin A and B. vulgaris extracts reduced blood glucose level, insulin, body weight and RBP4 expression before, during and after HFD. Furthermore, vitamin A reduced the blood glucose, triglycerides (TG) and cholesterol levels. IR syndrome associated with the RBP 4 alteration that gives high indication about the role of RBP4 expression in the IR progression and development. Furthermore, the treatment with vitamin A and/or b. vulgaris alleviated the IR syndrome through the action on RBP4 and Insulin secretion. On the other hand, vitamin A must be avoided for the predisposed IR and prediabetic patients. PMID:24191325

El-Sayed, Mohamed Mohammed; Ghareeb, Doaa Ahmad; Talat, Heba Allah; Sarhan, Eman Mohammed

2013-11-01

161

Antidiabetic Effect of Methanolic Extract from Berberis julianae Schneid. via Activation of AMP-Activated Protein Kinase in Type 2 Diabetic Mice  

PubMed Central

We have investigated the antidiabetic effect and mechanism of methanolic extract of Berberis julianae Schneid. (BJSME) in STZ induced Type 2 diabetes mellitus mice. T2DM mice were induced by high fat diet and low dose streptozotocin (STZ). BJSME was orally administrated at the doses of 60, 120, and 240?mg/kg/d, for 21 days. Metformin was used as positive control drug. Food intake, body weight, plasma glucose, oral glucose tolerance test, insulin tolerance test, insulin, and blood-lipid content were measured. The effects of BJSME on the glucose transporter 4 (GLUT4) translocation in L6 myotubes and the GLUT4 protein expression in skeletal muscle as well as phosphorylation of the AMP-activated protein kinase (AMPK) in liver and muscle were examined. In vitro and in vivo results indicate that BJSME increased GLUT4 translocation by 1.8-fold and BJSME significantly improved the oral glucose tolerance and low density lipoprotein cholesterol (LDL-C) of serum and reduced body weight, glucose, and other related blood-lipid contents. The BJSME treatment also stimulated the phosphorylation of AMPK. Thus, BJSME seems to possess promising beneficial effects for the treatment of T2DM with the possible mechanism via stimulating AMPK activity. PMID:25258641

Yang, Jing; Zhao, Ping; Wan, Dingrong; Zhou, Qi; Wang, Chao; Shu, Guangwen; Mei, Zhinan; Yang, Xinzhou

2014-01-01

162

Heterologous expression of two FAD-dependent oxidases with (S)-tetrahydroprotoberberine oxidase activity from Arge mone mexicana and Berberis wilsoniae in insect cells.  

PubMed

Berberine, palmatine and dehydrocoreximine are end products of protoberberine biosynthesis. These quaternary protoberberines are elicitor inducible and, like other phytoalexins, are highly oxidized. The oxidative potential of these compounds is derived from a diverse array of biosynthetic steps involving hydroxylation, intra-molecular C-C coupling, methylenedioxy bridge formation and a dehydrogenation reaction as the final step in the biosynthesis. For the berberine biosynthetic pathway, the identification of the dehydrogenase gene is the last remaining uncharacterized step in the elucidation of the biosynthesis at the gene level. An enzyme able to catalyze these reactions, (S)-tetrahydroprotoberberine oxidase (STOX, EC 1.3.3.8), was originally purified in the 1980s from suspension cells of Berberis wilsoniae and identified as a flavoprotein (Amann et al. 1984). We report enzymatic activity from recombinant STOX expressed in Spodoptera frugiperda Sf9 insect cells. The coding sequence was derived successively from peptide sequences of purified STOX protein. Furthermore, a recombinant oxidase with protoberberine dehydrogenase activity was obtained from a cDNA library of Argemone mexicana, a traditional medicinal plant that contains protoberberine alkaloids. The relationship of the two enzymes is discussed regarding their enzymatic activity, phylogeny and the alkaloid occurrence in the plants. Potential substrate binding and STOX-specific amino acid residues were identified based on sequence analysis and homology modeling. PMID:21327819

Gesell, Andreas; Chávez, Maria Luisa Díaz; Kramell, Robert; Piotrowski, Markus; Macheroux, Peter; Kutchan, Toni M

2011-06-01

163

Antidiabetic Effect of Methanolic Extract from Berberis julianae Schneid. via Activation of AMP-Activated Protein Kinase in Type 2 Diabetic Mice.  

PubMed

We have investigated the antidiabetic effect and mechanism of methanolic extract of Berberis julianae Schneid. (BJSME) in STZ induced Type 2 diabetes mellitus mice. T2DM mice were induced by high fat diet and low dose streptozotocin (STZ). BJSME was orally administrated at the doses of 60, 120, and 240?mg/kg/d, for 21 days. Metformin was used as positive control drug. Food intake, body weight, plasma glucose, oral glucose tolerance test, insulin tolerance test, insulin, and blood-lipid content were measured. The effects of BJSME on the glucose transporter 4 (GLUT4) translocation in L6 myotubes and the GLUT4 protein expression in skeletal muscle as well as phosphorylation of the AMP-activated protein kinase (AMPK) in liver and muscle were examined. In vitro and in vivo results indicate that BJSME increased GLUT4 translocation by 1.8-fold and BJSME significantly improved the oral glucose tolerance and low density lipoprotein cholesterol (LDL-C) of serum and reduced body weight, glucose, and other related blood-lipid contents. The BJSME treatment also stimulated the phosphorylation of AMPK. Thus, BJSME seems to possess promising beneficial effects for the treatment of T2DM with the possible mechanism via stimulating AMPK activity. PMID:25258641

Yang, Jing; Zhao, Ping; Wan, Dingrong; Zhou, Qi; Wang, Chao; Shu, Guangwen; Mei, Zhinan; Yang, Xinzhou

2014-01-01

164

0 0.1 0.2 0.3 Av.LAM/deg.  

E-print Network

as a function of plastic strain. Methodology: EBSD Measurement Qualitative Results Quantitative Results Fig. 3 stress of material) Why EBSD? · Versatile: measures microstructural changes, strain and defects0.0 0.2 0.4 0.6 0.8 1.0 0 0.1 0.2 0.3 Av.LAM/deg. Plastic strain Average local misorientation

Bandara, Arosha

165

Isolation and characterization of a flocculating protein from Moringa oleifera Lam  

Microsoft Academic Search

A flocculating protein from the seeds of Moringa oleifera Lam. was isolated by extraction with phosphate buffer followed by cation exchange chromatography. The molecular mass of the protein determined by SDS-PAGE was about 6.5 kDa, the isoelectric point was above pH 10. Amino acid analysis and sequencing showed high contents of glutamine, arginine and proline, and a total of 60

Ursula Gassenschmidt; Klaus D. Jany; Tauscher Bernhard; Heinz Niebergall

1995-01-01

166

Anti-fungal activity of crude extracts and essential oil of Moringa oleifera Lam.  

PubMed

Investigations were carried out to evaluate the therapeutic properties of the seeds and leaves of Moringa oleifera Lam as herbal medicines. Ethanol extracts showed anti-fungal activities in vitro against dermatophytes such as Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum, and Microsporum canis. GC-MS analysis of the chemical composition of the essential oil from leaves showed a total of 44 compounds. Isolated extracts could be of use for the future development of anti-skin disease agents. PMID:16406607

Chuang, Ping-Hsien; Lee, Chi-Wei; Chou, Jia-Ying; Murugan, M; Shieh, Bor-Jinn; Chen, Hueih-Min

2007-01-01

167

Molecular Characteristics of the Mycobacterium tuberculosis LAM-RUS Family Prevalent in Central Russia? †  

PubMed Central

We analyzed IS6110-associated polymorphisms in the phospholipase C genes of 107 isolates of Mycobacterium tuberculosis selected to be representative of isolates circulating in central Russia. We found that the majority of Latin American-Mediterranean family strains contained an insertion in a unique position in the plcA gene, suggesting a common ancestor. This insertion can serve as a specific genetic marker for this group, which we designate the LAM-RUS family. PMID:17942651

Dubiley, Svetlana; Kirillov, Eugene; Ignatova, Anna; Stepanshina, Valentina; Shemyakin, Igor

2007-01-01

168

Establishment of Carex stricta Lam. seedlings in experimental wetlands with implications for restoration  

Microsoft Academic Search

The loss of Carex dominated meadows due to agricultural drainage in the previously glaciated midcontinent of North America has been extensive.\\u000a The lack of natural Carex recruitment in wetland restorations and the failures of revegetation attempts underscore the need for information on the\\u000a establishment requirements of wetland sedges. In this study, seedlings of Carex stricta Lam. were planted in three

Rachel A. Budelsky; Susan M. Galatowitsch

2004-01-01

169

Optimisation of somatic embryogenesis in fourteen cultivars of sweet potato [Ipomoea batatas (L.) Lam.  

Microsoft Academic Search

Culture procedures have been developed to facilitate the induction and maintenance of somatic embryogenic tissues in 14 out\\u000a of 16 tested cultivars of sweet potato [Ipomoea batatas (L.) Lam]. Both the size of the axillary bud explant and the type of auxin were found to be critical for the successful induction\\u000a of somatic embryogenesis. Of the five auxins screened 2,4-dichlorophenoxyacetic

S. Al-Mazrooei; M. H. Bhatti; G. G. Henshaw; N. J. Taylor; D. Blakesley

1997-01-01

170

PREDICTING WATER ACTIVITY IN ELECTROLYTE SOLUTIONS WITH THE CISTERNAS-LAM MODEL  

SciTech Connect

Water activity is an important parameter needed to predict the solubility of hydrated salts in Hanford nuclear waste supernatants. A number of models available in the scientific literature predict water activity from electrolyte solution composition. The Cisternas-Lam model is one of those models and has several advantages for nuclear waste application. One advantage is that it has a single electrolyte specific parameter that is temperature independent. Thus, this parameter can be determined from very limited data and extrapolated widely. The Cisternas-Lam model has five coefficients that are used for all aqueous electrolytes. The present study aims to determine if there is a substantial improvement in making all six coefficients electrolyte specific. The Cisternas-Lam model was fit to data for six major electrolytes in Hanford nuclear waste supernatants. The model was first fit to all data to determine the five global coefficients, when they were held constant for all electrolytes it yielded a substantially better fit. Subsequently, the model was fit to each electrolyte dataset separately, where all six coefficients were allowed to be electrolyte specific. Treating all six coefficients as electrolyte specific did not make sufficient difference, given the complexity of applying the electrolyte specific parameters to multi-solute systems. Revised water specific parameters, optimized to the electrolytes relevant to Hanford waste, are also reported.

REYNOLDS JG; GREER DA; DISSELKAMP RL

2011-03-01

171

Interaction of Bacteriophage ? with Its E. coli Receptor, LamB  

PubMed Central

The initial step of viral infection is the binding of a virus onto the host cell surface. This first viral-host interaction would determine subsequent infection steps and the fate of the entire infection process. A basic understating of the underlining mechanism of initial virus-host binding is a prerequisite for establishing the nature of viral infection. Bacteriophage ? and its host Escherichia coli serve as an excellent paradigm for this purpose. ? phages bind to specific receptors, LamB, on the host cell surface during the infection process. The interaction of bacteriophage ? with the LamB receptor has been the topic of many studies, resulting in wealth of information on the structure, biochemical properties and molecular biology of this system. Recently, imaging studies using fluorescently labeled phages and its receptor unveil the role of spatiotemporal dynamics and divulge the importance of stochasticity from hidden variables in the infection outcomes. The scope of this article is to review the present state of research on the interaction of bacteriophage ? and its E. coli receptor, LamB. PMID:23202520

Chatterjee, Sujoy; Rothenberg, Eli

2012-01-01

172

Human antigen-specific memory T cells express the homing receptor (LAM-1) necessary for lymphocyte recirculation.  

PubMed

Lymphocytes must circulate from blood into lymphoid tissues and sites of infection and inflammation to function efficiently in vivo. This process of "homing" is in part directed by the expression of the leukocyte adhesion molecule (LAM-1, also known as TQ1 and Leu-8) in humans and the homologous MEL-14 antigen in mice. In this report, we demonstrate that the LAM-1 molecule is a 74-kDa protein and that only half of the CD4+ T cells in humans which have a memory phenotype (CD45RA -CD29hi) express the LAM-1 molecule. Functionally, these two phenotypically distinct subpopulations of memory cells were quite different. The LAM-1+ memory cells proliferated better to recall antigen and induced three to seven times higher levels of B cell immunoglobulin secretion than their LAM-1- counterparts. Thus, antigen-specific memory T cells within the helper lineage express the homing receptor appropriate for regulating their migration to secondary lymphoid tissues and sites of inflammation. PMID:1695155

Tedder, T F; Matsuyama, T; Rothstein, D; Schlossman, S F; Morimoto, C

1990-06-01

173

Preventive Effects of Aqueous Extract of Berberis integerrima Bge. Root on Liver Injury Induced by Diabetes Mellitus (Type 1) in Rats  

PubMed Central

This study was conducted to assessthe preventive effect of aqueous extract of Berberis integerrima Bge. root (AEBIR) on liver damage and oxidative stress induced by diabetes mellitus in rats. Forty male rats were divided into 5 groups as follows: 1- normal (N); 2- normal + barberry (N+B) (they received barberry root extract for 6 weeks); 3- diabetic (D) (they received Streptozotocin (STZ), 65 mg/Kg BW /i.p.); 4- diabetic +barberry before (D+Bb) (they received barberry root extract for 3 weeks before STZ injection and continued for another three weeks); and 5- diabetic + barberry after (D+Ba) (three days after STZ injection, they received barberry root extract for 3 weeks). The experimental groups received barberry root extract (500 mg/Kg bw) intra gastric by gavage for 6 weeks. The treatment of diabetic rats with AEBIR showed a significant decreases(p<0.001) in levels of blood glucose, malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin while body weight, total protein, superoxide dismutase (SOD), catalase(CAT) and reduced glutathione (GSH) increased (p<0.001) in comparison to diabetic control rats. Consumption of AEBIR in group D+Bb caused significant improvement in all these factors, compared to the group D+Ba. Also in this study, for the first time, we demonstrated that administration of AEBIR before diabetes induction resulted in enhanced amelioration of liver complications compared to the group receiving it after induction, indicating that AEBIR can play a preventive role in such patients. PMID:25561940

Ashraf, Hossein; Zare, Samad

2015-01-01

174

Evaluation of the effects of hydroalcoholic extract of Berberis vulgaris root on the activity of liver enzymes in male hypercholesterolemic rats  

PubMed Central

Objectives: Hyperlipidemia can cause a variety of diseases such as atherosclerosis, diabetes, and fatty liver which is followed by increased liver enzymes. Since Berberis vulgaris (B. vulgaris) root possesses antioxidant properties, the present study was conducted to investigate the effect of its extract on the activity of liver enzymes in rats. Materials and Methods: In this experimental study, sixty Wistar rats were selected and allocated to six groups of ten each. The control group received a normal diet and the sham group received a fatty diet while the other groups including experimental groups received a fatty diet and the alcoholic extract of B. vulgaris at minimum (75 mg/kg), moderate (150 mg/kg), and maximum (300 mg/kg) doses by intraperitoneal injection (i.p.) or oral atorvastatin (10 mg /kg) with a fatty diet. At the end of this 21-day period, blood samples were drawn and the levels of the intended factors were measured. Data were analyzed using SPSS software version 11.5. Results: The comparison of the obtained results showed that the levels of alanine transaminase (ALT) and alkaline phosphatase (ALP) enzymes in the sham group that only received fatty food increased (p?0.05), whereas in the treatment groups receiving B. vulgaris extract as well as in the group receiving Atorvastatin, these enzymes significantly decreased; however, no significant changes were observed in aspartate transaminase (AST) levels. Conclusion: Noticing the antioxidant properties of B. vulgaris root extract and its effects on reducing the activity of liver enzymes, the extract of this plant can be a good choice for improving the function of liver. PMID:25050245

Taheri, Soheila; Zarei, Ali; Changizi Ashtiyani, Saeed; Rezaei, Azam; Zaheiri, Saeed

2012-01-01

175

Preventive Effects of Aqueous Extract of Berberis integerrima Bge. Root on Liver Injury Induced by Diabetes Mellitus (Type 1) in Rats.  

PubMed

This study was conducted to assessthe preventive effect of aqueous extract of Berberis integerrima Bge. root (AEBIR) on liver damage and oxidative stress induced by diabetes mellitus in rats. Forty male rats were divided into 5 groups as follows: 1- normal (N); 2- normal + barberry (N+B) (they received barberry root extract for 6 weeks); 3- diabetic (D) (they received Streptozotocin (STZ), 65 mg/Kg BW /i.p.); 4- diabetic +barberry before (D+Bb) (they received barberry root extract for 3 weeks before STZ injection and continued for another three weeks); and 5- diabetic + barberry after (D+Ba) (three days after STZ injection, they received barberry root extract for 3 weeks). The experimental groups received barberry root extract (500 mg/Kg bw) intra gastric by gavage for 6 weeks. The treatment of diabetic rats with AEBIR showed a significant decreases(p<0.001) in levels of blood glucose, malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin while body weight, total protein, superoxide dismutase (SOD), catalase(CAT) and reduced glutathione (GSH) increased (p<0.001) in comparison to diabetic control rats. Consumption of AEBIR in group D+Bb caused significant improvement in all these factors, compared to the group D+Ba. Also in this study, for the first time, we demonstrated that administration of AEBIR before diabetes induction resulted in enhanced amelioration of liver complications compared to the group receiving it after induction, indicating that AEBIR can play a preventive role in such patients. PMID:25561940

Ashraf, Hossein; Zare, Samad

2015-01-01

176

Analysis of hydroxycinnamic acids derivatives in calafate (Berberis microphylla G. Forst) berries by liquid chromatography with photodiode array and mass spectrometry detection.  

PubMed

Calafate (Berberis microphylla G. Forst) is a Patagonian barberry very rich in anthocyanins and one of the fruits with the highest levels of these polyphenols. Other phenolic compounds have also been described in calafate berries. However, to the best of our knowledge there is no available information on hydroxycinnamic acid derivatives. The complexity of hydroxycinnamic acids determination in calafate berries, due to their structure similarities and the interference of high anthocyanin concentration is addressed by means of solid liquid extraction, followed by solid phase extraction clean-up on MCX columns and HPLC-DAD-ESI-MS/MS. The optimized extraction, clean-up and HPLC separation method allowed the assignation of identity and quantification of 20 hydroxycinnamic acids from calafate fruits. 5-Caffeoylquinic acid was the main compound found in all the studied samples. Other 13 hydroxycinnamoyl quinic acids and 6 caffeic acid esters with aldaric acid derivatives assigned as glucaric acid were also identified. Moreover, the glucaric-based hydroxycinnamic acid derivatives accounted for almost the half of total content of this kind of phenolic compounds. The total concentration of hydroxycinnamic acids derivatives ranged between 0.32±0.00 ?mol/g and 8.28±0.01 ?mol/g. Effect of ripening and geographical location on hydroxycinnamic acid profiles and concentrations are also evaluated. The methodology allows the determination of hydroxycinnamic acids from calafate despite of the high anthocyanin concentrations, showing a much higher concentration of these acids than other widely consumed berries. Thus suggesting that calafate could be considered a very interesting fruit from the point of view of their nutraceutical composition. However, geographical location and ripening have incidence in levels of studied compounds. PMID:23398997

Ruiz, Antonieta; Mardones, Claudia; Vergara, Carola; Hermosín-Gutiérrez, Isidro; von Baer, Dietrich; Hinrichsen, Patricio; Rodriguez, Roberto; Arribillaga, Diego; Dominguez, Erwin

2013-03-15

177

Anthelmintic effects of Salix babylonica L. and Leucaena leucocephala Lam. extracts in growing lambs.  

PubMed

Twenty Katahdin?×?Pelibuey crossbreed male lambs, 3 to 4 months of age and 24?±?0.3 kg of body weight, were used to study the anthelmintic effects of administering extracts of Salix babylonica L. (SB) and Leucaena leucocephala Lam. (LL). Lambs had not been treated with anthelmintics previously and were randomly allocated into four groups of five lambs each in a completely randomized design. Treatments were as follows: control (lambs fed on total mixed ration without extracts), SB (as control plus S. babylonica L. extract at 30 ml/day), LL (as control plus L. leucocephala Lam. extract at 30 ml/day), and SBLL (as control plus 30 ml/day of S. babylonica L. and L. leucocephala Lam. extracts in a 1:1 (v/v) mixture) for 63 days. Extracts were orally administered before the 8:00 a.m. feeding to each lamb. Rectal fecal samples were collected from each lamb at day 22 (P1), day 43 (P2), and day 63 (P3) of the experiment. Adult worm and egg counts were determined in each fecal sample immediately after collection. Plant secondary metabolites of total phenolics, saponins, and the aqueous fraction were 50 % lower in the SB versus LL extracts. Overall, the oral administration of extracts has improved the egg and worm count reductions in lamb feces by 54, 47, and 40 % for LL, SB, and SBLL, respectively, versus the control lambs. Reductions of worm egg counts in lamb feces were higher (P?

Hernandez, Pablo Mejia; Salem, Abdelfattah Z M; Elghandour, Mona M M Y; Cipriano-Salazar, Moisés; Cruz-Lagunas, Blas; Camacho, Luis Miguel

2014-01-01

178

Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.  

PubMed Central

We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C3 neutralization epitope from poliovirus) into permissive sites of LamB (i.e., sites which tolerate insertions without deleterious effects on the protein activities or the cell). A specific monoclonal antibody was then used to examine the position of the inserted epitope with respect to the protein and the membrane. In the present work, we set up a site-directed procedure to insert the C3 epitope at new sites in order to distinguish between two-dimensional folding models. This allowed us to identify two new surface loops of LamB and to predict another periplasmic exposed region. The results obtained by random and directed epitope tagging are analyzed in light of the recently published X-ray structure of the LamB protein. Study of 23 hybrid LamB-C3 proteins led to the direct identification of five of the nine external loops (L4, L5, L6, L7, and L9) and led to the prediction of four periplasmic loops (I1, I4, I5, and I8) of LamB. Nine of the hybrid proteins did not lead to topological conclusions, and none led to the wrong predictions or conclusions. The comparison indicates that parts of models based on secondary structure predictions alone are not reliable and points to the importance of experimental data in the establishment of outer membrane protein topological models. The advantages and limitations of genetic foreign epitope insertion for the study of integral membrane proteins are discussed. PMID:8655540

Newton, S M; Klebba, P E; Michel, V; Hofnung, M; Charbit, A

1996-01-01

179

Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.  

PubMed

We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C3 neutralization epitope from poliovirus) into permissive sites of LamB (i.e., sites which tolerate insertions without deleterious effects on the protein activities or the cell). A specific monoclonal antibody was then used to examine the position of the inserted epitope with respect to the protein and the membrane. In the present work, we set up a site-directed procedure to insert the C3 epitope at new sites in order to distinguish between two-dimensional folding models. This allowed us to identify two new surface loops of LamB and to predict another periplasmic exposed region. The results obtained by random and directed epitope tagging are analyzed in light of the recently published X-ray structure of the LamB protein. Study of 23 hybrid LamB-C3 proteins led to the direct identification of five of the nine external loops (L4, L5, L6, L7, and L9) and led to the prediction of four periplasmic loops (I1, I4, I5, and I8) of LamB. Nine of the hybrid proteins did not lead to topological conclusions, and none led to the wrong predictions or conclusions. The comparison indicates that parts of models based on secondary structure predictions alone are not reliable and points to the importance of experimental data in the establishment of outer membrane protein topological models. The advantages and limitations of genetic foreign epitope insertion for the study of integral membrane proteins are discussed. PMID:8655540

Newton, S M; Klebba, P E; Michel, V; Hofnung, M; Charbit, A

1996-06-01

180

Phytochemical analysis, antimicrobial, antioxidant and urease inhibitory potential of Cyphostemma digitatum Lam.  

PubMed

In this paper we report the antimicrobial, antiradical and urease inhibitory potential along with photochemical investigation of the crude extracts of Cyphostemma digitatum Lam. Phytochemical screening of both the crude (hot/cold) alcoholic and aqueous extracts of C. digitatum showed the presence of alkaloids, flavonoids, saponins, coumarins, steroids, terpenoids and tannins. The crude methanolic extract (hot/cold) exhibited good antioxidant activity, while the aqueous extract was a weak antioxidant. The crude methanolic extract was found to be more active against Bacillus subtilis, while both the extracts showed moderate antifungal potential, the methanolic crude extract showed good urease inhibitory activity compared with the aqueous crude extract. PMID:25154866

Khan, Rasool; Saif, Abdullah Qasem; Quradha, Mohammad Mansour; Ali, Jawad; Rauf, Abdur

2015-03-01

181

Photoelectrochemical solar cell using extract of Eugenia jambolana Lam as a natural sensitizer.  

PubMed

The extract of Jambol o (java plum), Eugenia jambolana Lam, was used as a natural sensitizer of a wide band-gap semiconductor (TiO2) in photoelectrochemical solar cells. The natural dye, adsorbed onto the semiconductor surface, absorbs visible light and promotes electron transfer across the dye/semiconductor interface. Photogenerated current and voltage as high as 2.3 mA and 711 mV, respectively, were obtained and effective conversion of visible light into electricity was achieved. The use of a natural product as the semiconductor sensitizer enables a faster and simpler production of cheaper and environmentally friendly solar cells. PMID:12894301

Garcia, Christian G; Polo, André S; Iha, Neyde Y Murakami

2003-06-01

182

Stress polishing of E-ELT segment at LAM: full-scale demonstrator status  

NASA Astrophysics Data System (ADS)

The Laboratoire d'Astrophysique de Marseille (LAM) is involved in the prototyping of a full scale demonstrator for stress polishing of segments for the European Extremely Large Telescope (E-ELT). Stress polishing method is developed at LAM since more than 40 years, and this mature technology has recently been used with success for VLT instruments. Stress polishing is now considered as a promising manufacturing method for mass production of large off axis mirrors, specifically for ELT segments. This powerful method, based on elasticity theory, allows the generation of super-smooth off-axis aspherics with a minimal amount of high spatial frequency ripples by spherically polishing a warped blank with a full-sized tool. Thanks to the simple spherical polishing, the operation time can be strongly reduced compared to the time-consuming sub-aperture tool methods of grinding and polishing. The goal is to rapidly converge to less than 1 micron RMS of optical quality on a circular blank which will be finally cut hexagonally and finished using Ion Beam Finishing. In this paper we will present the status of the demonstrator and the design of the warping harness prototype that must be able to precisely warp the circular blank.

Laslandes, Marie; Rousselet, Nicolas; Ferrari, Marc; Hugot, Emmanuel; Floriot, Johan; Vivès, Sébastien; Lemaitre, Gérard; Carré, Jean François; Cayrel, Marc

2011-10-01

183

Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats.  

PubMed

This study was carried out to evaluate the effect of Moringa oleifera Lam (Moringa) seed extract on liver fibrosis. Liver fibrosis was induced by the oral administration of 20% carbon tetrachloride (CCl(4)), twice weekly and for 8 weeks. Simultaneously, M.oleifera Lam seed extract (1g/kg) was orally administered daily. The biochemical and histological results showed that Moringa reduced liver damage as well as symptoms of liver fibrosis. The administration of Moringa seed extract decreased the CCl(4)-induced elevation of serum aminotransferase activities and globulin level. The elevations of hepatic hydroxyproline content and myeloperoxidase activity were also reduced by Moringa treatment. Furthermore, the immunohistochemical study showed that Moringa markedly reduced the numbers of smooth muscle alpha-actin-positive cells and the accumulation of collagens I and III in liver. Moringa seed extract showed significant inhibitory effect on 1,1-diphenyl-2-picrylhydrazyl free radical, as well as strong reducing antioxidant power. The activity of superoxide dismutase as well as the content of both malondialdehyde and protein carbonyl, which are oxidative stress markers, were reversed after treatment with Moringa. Finally, these results suggested that Moringa seed extract can act against CCl(4)-induced liver injury and fibrosis in rats by a mechanism related to its antioxidant properties, anti-inflammatory effect and its ability to attenuate the hepatic stellate cells activation. PMID:19854235

Hamza, Alaaeldin A

2010-01-01

184

Effect of different growth stages of Ziziphora clinopodioides Lam. on its chemical composition  

PubMed Central

Aims: The aim of this study is to monitor the changes in the chemical composition of Ziziphora clinopodioides Lam. throughout nine different growth stages. Materials and Methods: Volatile components such as essential oils were analyzed using the gas chromatography (GC) and GC-mass spectrometry, and the contents of non-volatile components were determined by a visible spectrophotometer. Results: Hydro-distilled essential oil content ranged from a minimum of 1.1% (in the post-flowering stage) to a maximum of 1.8% (in the flowering stage). The essential oils included pulegone, which was the most abundant component (77.48-87.3%), p-menthanone (2.79-12.39%), trans-isopulegone (1.04-2.06%), d-limonene (0.51-3.03%) and eucarvone (1.5-4.48%). The contents of non-volatile components, such as that of total phenolics (TPC), total flavonoids (TFC), total triterpenoids content (TTC) and total free amino acids content (TFAAC) were measured using visible spectrophotometry. In the growing stage, TPC, TFC, TTC and TFAAC were 9.91-12.80 mg/g, 29.84-50.63 mg/g, 0.57-1.41 mg/g and 13.33-28.56 mg/g, respectively. Conclusion: These data can be used as a basis to determine the optimal harvest time of Z. clinopodioide Lam. PMID:24914287

Ding, Wenhuan; Yang, Tao; Liu, Feng; Tian, Shuge

2014-01-01

185

Identifying and selecting for genetic diversity in Papua New Guinea sweetpotato Ipomoea batatas (L.) Lam. germplasm collected as botanical seed  

Technology Transfer Automated Retrieval System (TEKTRAN)

Genetic analysis was conducted on 141 "Ipomoea batatas" L. (Lam.) Genotypes derived from botanical seed originally collected from 26 sites in 4 provinces in Papua New Guinea. Relatedness among accessions was estimated by analysis of the AFLP data using the Dice coefficient of similarity and UPGMA. ...

186

The use of polymer solutions for deep excavations Dr Carlos Lam, Lecturer in Geotechnical Engineering, University of Manchester  

E-print Network

The use of polymer solutions for deep excavations Dr Carlos Lam, Lecturer in Geotechnical of Engineering ABSTRACT Polymer solutions have been used as support fluids in diaphragm walling and bored piling a particular aspect of polymer fluids ­ the potential for better shaft resistance of piles. This talk will give

Davies, Christopher

187

ReTAX+: A Cooperative Taxonomy Revision Tool Sik Chun (Joey) Lam, Derek Sleeman, and Wamberto Vasconcelos  

E-print Network

1 ReTAX+: A Cooperative Taxonomy Revision Tool Sik Chun (Joey) Lam, Derek Sleeman, and Wamberto in an established taxonomy; it suggests a number of ways in which that can be achieved namely; modifying the new entity, the taxonomy, or both. Further, a set of refinement operators are used to guarantee

Vasconcelos, Wamberto

188

UHF RFID TAG CHARACTERIZATION: OVERVIEW AND STATE-OF-THE-ART Pavel Nikitin, KVS Rao, Sander Lam  

E-print Network

UHF RFID TAG CHARACTERIZATION: OVERVIEW AND STATE-OF-THE-ART Pavel Nikitin, KVS Rao, Sander Lam an overview of UHF RFID tag performance characterization. We review the link budget of RFID system, explain-of-the art test systems present on the market today. Keywords: Measurement, RFID 1. Introduction Radio

Hochberg, Michael

189

Low Cost Silver Ink RFID Tag Antennas Pavel V. Nikitin, Sander Lam, and K. V. S. Rao  

E-print Network

Low Cost Silver Ink RFID Tag Antennas Pavel V. Nikitin, Sander Lam, and K. V. S. Rao Intermec for manufacturing RFID tags at Ultra High Frequencies (UHF). An analysis of two different RFID tag an- tennas made from the results that RFID tag performance depends both on material and on the shape of the antenna

Hannaford, Blake

190

Silk: A Resilient Routing Fabric for Peer-to-Peer Networks Simon S. Lam and Huaiyu Liu  

E-print Network

Silk: A Resilient Routing Fabric for Peer-to-Peer Networks£ Simon S. Lam and Huaiyu Liu Dept named Silk, will be referred to as Silk pro- tocols. From simulation experiments in which the number of concurrent joins and failures was up to 50% of the initial net- work size, we found that, for � ¾, Silk

Lam, Simon S.

191

SmartTunnel: Achieving Reliability in the Internet Yi Li, Yin Zhang, LiLi Qiu, Simon Lam  

E-print Network

1 SmartTunnel: Achieving Reliability in the Internet Yi Li, Yin Zhang, LiLi Qiu, Simon Lam's Internet. In this paper, we propose SmartTunnel, an end-to-end approach to achieving reliability. A Smart-to-end reliability. Unfor- tunately, today's Internet does not even provide two 9s (99%) reliability [16], [10], [8

Lam, Simon S.

192

Screening antimutagenic and antiproliferative properties of extracts isolated from Jackfruit pulp (Artocarpus heterophyllus Lam).  

PubMed

The present focused on the study of the antimutagenic and antiproliferative potential of pulp Jackfruit (Artocarpus heterophyllus Lam) extract, using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line M12.C3.F6 (murine B-cell lymphoma), respectively. Jackfruit pulp extract was sequentially fractionated by chromatography (RP-HPLC) and each fraction was tested for antimutagenic and antiproliferative activities. The organic extracts obtained from Jackfruit pulp reduced the number of revertants caused by aflatoxin B1 (AFB1) and proliferation of cells M12.C3.F6; a dose-response relationship was showed. Sequential RP-HPLC fractionation of the active extracts produced both antimutagenic and/or antiproliferative fractions. These results suggested that the Jackfruit contained compounds with chemoprotective properties to reduce the mutagenicity of AFB1, also proliferation of a cancer cell line. PMID:25577099

Ruiz-Montañez, G; Burgos-Hernández, A; Calderón-Santoyo, M; López-Saiz, C M; Velázquez-Contreras, C A; Navarro-Ocaña, A; Ragazzo-Sánchez, J A

2015-05-15

193

Predicting weed migration from soil and climate maps. [Centaurea maculosa Lam  

SciTech Connect

Soil characteristics, elevation, annual precipitation, potential evapotranspiration, length of frost-free season, and mean maximum July temperature were estimated for 116 established infestations of spotted knapweed (Centaurea maculosa Lam. number/sup 3/ CENMA) in Montana using basic land resource maps. Areas potentially vulnerable to invasion by the plant were delineated on the basis of representative edaphic and climatic characteristics. No single environmental variable was an effective predictor of sites vulnerable to invasion by spotted knapweed. Only a combination of variables was effective, indicating that the factors that regulate adaptability of this plant are complex. This technique provides a first approximation map of the regions most similar environmentally to infested sites and; therefore, most vulnerable to further invasion. This weed migration prediction technique shows promise for predicting suitable habitats of other invader species. 6 references, 4 figures, 1 table.

Chicoine, T.K.; Fay, P.K.; Nielsen, G.A.

1985-01-01

194

Structural and biochemical characterization of the laminarinase ZgLamCGH16 from Zobellia galactanivorans suggests preferred recognition of branched laminarin.  

PubMed

Laminarin is a ?-1,3-D-glucan displaying occasional ?-1,6 branches. This storage polysaccharide of brown algae constitutes an abundant source of carbon for marine bacteria such as Zobellia galactanivorans. This marine member of the Bacteroidetes possesses five putative ?-1,3-glucanases [four belonging to glycosyl hydrolase family 16 (GH16) and one to GH64] with various modular architectures. Here, the characterization of the ?-glucanase ZgLamC is reported. The catalytic GH16 module (ZgLamCGH16) was produced in Escherichia coli and purified. This recombinant enzyme has a preferential specificity for laminarin but also a significant activity on mixed-linked glucan (MLG). The structure of an inactive mutant of ZgLamCGH16 in complex with a thio-?-1,3-hexaglucan substrate unravelled a straight active-site cleft with three additional pockets flanking subsites -1, -2 and -3. These lateral pockets are occupied by a glycerol, an acetate ion and a chloride ion, respectively. The presence of these molecules in the vicinity of the O6 hydroxyl group of each glucose moiety suggests that ZgLamCGH16 accommodates branched laminarins as substrates. Altogether, ZgLamC is a secreted laminarinase that is likely to be involved in the initial step of degradation of branched laminarin, while the previously characterized ZgLamA efficiently degrades unbranched laminarin and oligo-laminarins. PMID:25664729

Labourel, Aurore; Jam, Murielle; Legentil, Laurent; Sylla, Balla; Hehemann, Jan Hendrik; Ferrières, Vincent; Czjzek, Mirjam; Michel, Gurvan

2015-02-01

195

Chemomodulatory Effect of Moringa Oleifera, Lam, on Hepatic Carcinogen Metabolising Enzymes, Antioxidant Parameters and Skin Papillomagenesis in Mice  

Microsoft Academic Search

The modulatory effects of a hydro-alchoholic extract of drumsticks of Moringa oliefera Lam at doses of 125 mg\\/ kg bodyweight and 250 mg\\/ kg body weight for 7 and 14 days, respectively, were investigated with reference to drug metabolising Phase I (Cytochrome b 5 and Cytochrome P 450 ) and Phase II (Glutathione-S- transferase) enzymes, anti-oxidant enzymes, glutathione content and

Rupjyoti Bharali; Jawahira Tabassum; Mohammed Rekibul; Haque Azad

2003-01-01

196

Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats  

Microsoft Academic Search

The leaves of Moringa oleifera Lam (Moringaceae) are used by the Indians in their herbal medicine as a hypocholesterolemic agent in obese patients. The scientific basis for their use in hypercholesterolemia was therefore examined. It was found that administration of the crude leaf extract of Moringa oleifera along with high-fat diet decreased the high-fat diet-induced increases in serum, liver, and

S Ghasi; E Nwobodo; J. O Ofili

2000-01-01

197

Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweet potato feathery mottle virus  

Microsoft Academic Search

One of the most-serious diseases of sweet potato [Ipomoea batatas (L.) Lam] is russet crack disease caused by sweet potato feathery mottle virus (SPFMV). We constructed an expression vector carrying the coat protein (CP) and hygromycin phosphotransferase (hpt) genes\\u000a driven by cauliflower mosaic virus 35 S promoters. Accordingly, we introduced the expression vector into sweet potato variety\\u000a Chikei 682-11 by

Y. Okada; A. Saito; M. Nishiguchi; T. Kimura; M. Mori; K. Hanada; J. Sakai; C. Miyazaki; Y. Matsuda; T. Murata

2001-01-01

198

A valued Indian medicinal plant - Begonia malabarica Lam. : Successful plant regeneration through various explants and field performance  

Microsoft Academic Search

A cost-effective and efficient protocol has been described in the present work for large- scale and rapid in vitro propagation of a valuable medicinal herb Begonia malabarica Lam. (Begoniaceae) by shoot auxillary-bud proliferation and organogenesis on MS medium supplemented with 6-benzylaminopurine (BA; 0.0-8.8 mg\\/l) and indole-3-acetic acid (IAA; 0.0-2.88 mg\\/l) at different concentrations, either alone or in combinations. Initiation of

Mathan C. Nisha; Sevanan Rajeshkumar; Thangavel Selvaraj

199

EFECTO PERJUDICIAL DE Moringa oleifera (Lam.) COMBINADA CON OTROS DESECHOS AGRÍCOLAS COMO SUSTRATOS PARA LA LOMBRIZ ROJA (Eisenia spp.)  

Microsoft Academic Search

SUMMARY An experiment was carried out to study the behavior of the red worm (Eisenia spp.) fed with 5 nutritious substrates based in Moringa oleifera (Lam.) foliage, for the nutritional potentiali- ties, and other agricultural wastes in Trujillo state, Venezuela. A randomized design with repeated measurements and three repli- cates was used. Initially, 1.16kg of biomass in 0.5m3 of substrate

Luis José Cov; Danny Eugenio García; Alexander Rafael Castro; María Gabriela Medina

2007-01-01

200

Increased resistance to crown rust disease in transgenic Italian ryegrass ( Lolium multiflorum Lam.) expressing the rice chitinase gene  

Microsoft Academic Search

We introduced the rice chitinase (Cht-2; RCC2) gene into calli of Italian ryegrass (Lolium multiflorum Lam.), with a hygromycin phosphotransferase (HPT) gene as a selectable marker, by particle bombardment. Hygromycin-resistant calli were selected and transferred to regeneration medium for shoot formation. Polymerase chain reaction (PCR) analysis revealed regenerants containing the HPT gene. The RCC2 gene was detected in 65.5% of

Wataru Takahashi; Masahiro Fujimori; Yuichi Miura; Toshinori Komatsu; Yoko Nishizawa; Tadaaki Hibi; Tadashi Takamizo

2005-01-01

201

The C-terminal portion of the tail fiber protein of bacteriophage lambda is responsible for binding to LamB, its receptor at the surface of Escherichia coli K-12.  

PubMed

Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residues of J could bind to LamB trimers and inhibited recognition by anti-LamB antibodies. Electron microscopy further demonstrated that the fusion protein could also bind to LamB at the surface of intact cells. This interaction prevented lambda adsorption but affected only partially maltose uptake. PMID:10629200

Wang, J; Hofnung, M; Charbit, A

2000-01-01

202

The C-Terminal Portion of the Tail Fiber Protein of Bacteriophage Lambda Is Responsible for Binding to LamB, Its Receptor at the Surface of Escherichia coli K-12  

PubMed Central

Bacteriophage ? adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of ?, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residues of J could bind to LamB trimers and inhibited recognition by anti-LamB antibodies. Electron microscopy further demonstrated that the fusion protein could also bind to LamB at the surface of intact cells. This interaction prevented ? adsorption but affected only partially maltose uptake. PMID:10629200

Wang, Jiang; Hofnung, Maurice; Charbit, Alain

2000-01-01

203

A role for residue 151 of LamB in bacteriophage lambda adsorption: possible steric effect of amino acid substitutions.  

PubMed Central

LamB is the cell surface receptor for bacteriophage lambda. LamB missense mutations yielding resistance to lambda have been previously grouped in two classes. Class I mutants block growth of lambda with wild-type host range (lambda h+) but support growth of one-step extended-host-range mutants (lambda h). Class II mutants block lambda h but support growth of two-step extended host range mutants (lambda hh*). While Class I mutations occur at 11 different amino acid sites, in five distinct portions of LamB, all the Class II mutations analyzed previously correspond to the same G-to-D change at amino acid 151. We generated by in vitro mutagenesis four different new substitutions at site 151 (to S, V, R, and C). Two of the mutants (G-151-->V [G151V] and G151R) were of Class II, while the two others (G151S and G151C) were of Class I, demonstrating that not only the site but also the nature of the substitutions at residue 151 was critical for the phage sensitivity phenotypes. The introduction of a negatively charged, a positively charged, or an aliphatic nonpolar residue at site 151 of LamB prevented both lambda h+ and lambda h adsorption, indicating that the block is not due to a charge effect. In contrast to G151D, which was sensitive to all the lambda hh* phages, G151V and G151R conferred sensitivity to only four of the five lambda hh* phages. Thus, G151V and G151R represent a new subclass of Class II LamB mutations that is more restrictive with respect to the growth of lambda hh*. Our results agree with the hypothesis that residue 151 belongs to an accessibility gate controlling the access to the phage tight-binding site and that substitutions at this residue affect the access of the phage to the binding site in relation to the size of the substitute side chain (surface area): the most restrictive changes are G151V and G151R, followed to a lesser extent by G151D and they by G151S and G151C. Images PMID:8195074

Charbit, A; Werts, C; Michel, V; Klebba, P E; Quillardet, P; Hofnung, M

1994-01-01

204

A role for residue 151 of LamB in bacteriophage lambda adsorption: possible steric effect of amino acid substitutions.  

PubMed

LamB is the cell surface receptor for bacteriophage lambda. LamB missense mutations yielding resistance to lambda have been previously grouped in two classes. Class I mutants block growth of lambda with wild-type host range (lambda h+) but support growth of one-step extended-host-range mutants (lambda h). Class II mutants block lambda h but support growth of two-step extended host range mutants (lambda hh*). While Class I mutations occur at 11 different amino acid sites, in five distinct portions of LamB, all the Class II mutations analyzed previously correspond to the same G-to-D change at amino acid 151. We generated by in vitro mutagenesis four different new substitutions at site 151 (to S, V, R, and C). Two of the mutants (G-151-->V [G151V] and G151R) were of Class II, while the two others (G151S and G151C) were of Class I, demonstrating that not only the site but also the nature of the substitutions at residue 151 was critical for the phage sensitivity phenotypes. The introduction of a negatively charged, a positively charged, or an aliphatic nonpolar residue at site 151 of LamB prevented both lambda h+ and lambda h adsorption, indicating that the block is not due to a charge effect. In contrast to G151D, which was sensitive to all the lambda hh* phages, G151V and G151R conferred sensitivity to only four of the five lambda hh* phages. Thus, G151V and G151R represent a new subclass of Class II LamB mutations that is more restrictive with respect to the growth of lambda hh*. Our results agree with the hypothesis that residue 151 belongs to an accessibility gate controlling the access to the phage tight-binding site and that substitutions at this residue affect the access of the phage to the binding site in relation to the size of the substitute side chain (surface area): the most restrictive changes are G151V and G151R, followed to a lesser extent by G151D and they by G151S and G151C. PMID:8195074

Charbit, A; Werts, C; Michel, V; Klebba, P E; Quillardet, P; Hofnung, M

1994-06-01

205

Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione level.  

PubMed

Initiation of acetaminophen (APAP) toxicities is believed to be promoted by oxidative stress during the event of overdosage. The aim of the present study was to evaluate the hepatoprotective action of Moringa oleifera Lam (MO), an Asian plant of high medicinal value, against a single high dose of APAP. Groups of five male Sprague-Dawley rats were pre-administered with MO (200 and 800 mg/kg) prior to a single dose of APAP (3g/kg body weight; p.o). Silymarin was used as an established hepatoprotective drug against APAP induced liver injury. The hepatoprotective activity of MO extract was observed following significant histopathological analysis and reduction of the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in groups pretreated with MO compared to those treated with APAP alone. Meanwhile, the level of glutathione (GSH) was found to be restored in MO-treated animals compared to the groups treated with APAP alone. These observations were comparable to the group pretreated with silymarin prior to APAP administration. Group that was treated with APAP alone exhibited high level of transaminases and ALP activities besides reduction in the GSH level. The histological hepatocellular deterioration was also evidenced. The results from the present study suggested that the leaves of MO can prevent hepatic injuries from APAP induced through preventing the decline of glutathione level. PMID:18514995

Fakurazi, S; Hairuszah, I; Nanthini, U

2008-08-01

206

Disentangling the Origins of Cultivated Sweet Potato (Ipomoea batatas (L.) Lam.)  

PubMed Central

Sweet potato (Ipomoea batatas (L.) Lam., Convolvulaceae) counts among the most widely cultivated staple crops worldwide, yet the origins of its domestication remain unclear. This hexaploid species could have had either an autopolyploid origin, from the diploid I. trifida, or an allopolyploid origin, involving genomes of I. trifida and I. triloba. We generated molecular genetic data for a broad sample of cultivated sweet potatoes and its diploid and polyploid wild relatives, for noncoding chloroplast and nuclear ITS sequences, and nuclear SSRs. Our data did not support an allopolyploid origin for I. batatas, nor any contribution of I. triloba in the genome of domesticated sweet potato. I. trifida and I. batatas are closely related although they do not share haplotypes. Our data support an autopolyploid origin of sweet potato from the ancestor it shares with I. trifida, which might be similar to currently observed tetraploid wild Ipomoea accessions. Two I. batatas chloroplast lineages were identified. They show more divergence with each other than either does with I. trifida. We thus propose that cultivated I. batatas have multiple origins, and evolved from at least two distinct autopolyploidization events in polymorphic wild populations of a single progenitor species. Secondary contact between sweet potatoes domesticated in Central America and in South America, from differentiated wild I. batatas populations, would have led to the introgression of chloroplast haplotypes of each lineage into nuclear backgrounds of the other, and to a reduced divergence between nuclear gene pools as compared with chloroplast haplotypes. PMID:23723970

Roullier, Caroline; Duputié, Anne; Wennekes, Paul; Benoit, Laure; Fernández Bringas, Víctor Manuel; Rossel, Genoveva; Tay, David; McKey, Doyle; Lebot, Vincent

2013-01-01

207

Disentangling the origins of cultivated sweet potato (Ipomoea batatas (L.) Lam.).  

PubMed

Sweet potato (Ipomoea batatas (L.) Lam., Convolvulaceae) counts among the most widely cultivated staple crops worldwide, yet the origins of its domestication remain unclear. This hexaploid species could have had either an autopolyploid origin, from the diploid I. trifida, or an allopolyploid origin, involving genomes of I. trifida and I. triloba. We generated molecular genetic data for a broad sample of cultivated sweet potatoes and its diploid and polyploid wild relatives, for noncoding chloroplast and nuclear ITS sequences, and nuclear SSRs. Our data did not support an allopolyploid origin for I. batatas, nor any contribution of I. triloba in the genome of domesticated sweet potato. I. trifida and I. batatas are closely related although they do not share haplotypes. Our data support an autopolyploid origin of sweet potato from the ancestor it shares with I. trifida, which might be similar to currently observed tetraploid wild Ipomoea accessions. Two I. batatas chloroplast lineages were identified. They show more divergence with each other than either does with I. trifida. We thus propose that cultivated I. batatas have multiple origins, and evolved from at least two distinct autopolyploidization events in polymorphic wild populations of a single progenitor species. Secondary contact between sweet potatoes domesticated in Central America and in South America, from differentiated wild I. batatas populations, would have led to the introgression of chloroplast haplotypes of each lineage into nuclear backgrounds of the other, and to a reduced divergence between nuclear gene pools as compared with chloroplast haplotypes. PMID:23723970

Roullier, Caroline; Duputié, Anne; Wennekes, Paul; Benoit, Laure; Fernández Bringas, Víctor Manuel; Rossel, Genoveva; Tay, David; McKey, Doyle; Lebot, Vincent

2013-01-01

208

Achyrocline satureioides (Lam.) D.C. Hydroalcoholic Extract Inhibits Neutrophil Functions Related to Innate Host Defense  

PubMed Central

Achyrocline satureioides (Lam.) D.C. is a herb native to South America, and its inflorescences are popularly employed to treat inflammatory diseases. Here, the effects of the in vivo actions of the hydroalcoholic extract obtained from inflorescences of A. satureioides on neutrophil trafficking into inflamed tissue were investigated. Male Wistar rats were orally treated with A. satureioides extract, and inflammation was induced one hour later by lipopolysaccharide injection into the subcutaneous tissue. The number of leukocytes and the amount of chemotactic mediators were quantified in the inflammatory exudate, and adhesion molecule and toll-like receptor 4 (TLR-4) expressions and phorbol-myristate-acetate- (PMA-) stimulated oxidative burst were quantified in circulating neutrophils. Leukocyte-endothelial interactions were quantified in the mesentery tissue. Enzymes and tissue morphology of the liver and kidney were evaluated. Treatment with A. satureioides extract reduced neutrophil influx and secretion of leukotriene B4 and CINC-1 in the exudates, the number of rolling and adhered leukocytes in the mesentery postcapillary venules, neutrophil L-selectin, ?2-integrin and TLR-4 expression, and oxidative burst, but did not cause an alteration in the morphology and activities of liver and kidney. Together, the data show that A. satureioides extract inhibits neutrophil functions related to the innate response and does not cause systemic toxicity. PMID:23476704

Barioni, Eric Diego; Machado, Isabel Daufenback; Rodrigues, Stephen Fernandes de Paula; Ferraz-de-Paula, Viviane; Wagner, Theodoro Marcel; Cogliati, Bruno; Corrêa dos Santos, Matheus; Machado, Marina da Silva; de Andrade, Sérgio Faloni; Niero, Rivaldo; Farsky, Sandra Helena Poliselli

2013-01-01

209

Purification and structural characterization of an ?-glucosidase inhibitory polysaccharide from apricot (Armeniaca sibirica L. Lam.) pulp.  

PubMed

In this study, the crude polysaccharide (APPS) from the fruiting bodies of apricot (Armeniaca sibirica L. Lam.) was isolated and fractionated by ultrafiltration and Sephadex G-75 gel chromatography. The hypoglycemic activities of all fractions were determined by ?-glucosidase inhibitory activity in vitro. The fraction APPS1-2 showed the best activity with an IC50 of 6.06mg/mL. The properties and chemical compositions of this fraction were analyzed with high-performance liquid chromatography, gel permeation chromatography-eighteen angle laser light scattering instrument, UV spectroscopy, infrared spectroscopy, and NMR spectroscopy ((1)H). The results demonstrated that APPS1-2 was a neutral glycoconjugate with a molecular weight of 25.93kDa. It comprised rhamnose, glucose, mannose, and galactose, with a relative molar ratio of 1.34:2.01:0.48:0.35. The backbone of APPS1-2 may consist of rhamnose and glucose, but its branches may consist of mannose and galactose. The IR and UV spectrum of APPS1-2 revealed the typical characteristics of heteropolysaccharide. (1)H NMR spectrum showed that APPS1-2 contained ?-configurations. PMID:25659703

Cui, Jie; Gu, Xin; Wang, Fengjun; Ouyang, Jie; Wang, Jianzhong

2015-05-01

210

Simultaneous HPLC quantitative analysis of active compounds in leaves of Moringa oleifera Lam.  

PubMed

Moringa oleifera Lam. has been used as a traditional medicine for the treatment of numerous diseases. A simultaneous high-performance liquid chromatography (HPLC) analysis was developed and validated for the determination of the contents of crypto-chlorogenic acid, isoquercetin and astragalin, the primary antioxidative compounds, in M. oleifera leaves. HPLC analysis was successfully conducted by using a Hypersil BDS C18 column, eluted with a gradient of methanol-1% acetic acid with a flow rate of 1 mL/min, and detected at 334 nm. Parameters for the validation included linearity, precision, accuracy and limits of detection and quantitation. The developed HPLC method was precise, with relative standard deviation < 2%. The recovery values of crypto-chlorogenic acid, isoquercetin and astragalin in M. oleifera leaf extracts were 98.50, 98.47 and 98.59%, respectively. The average contents of these compounds in the dried ethanolic extracts of the leaves of M. oleifera collected from different regions of Thailand were 0.081, 0.120 and 0.153% (w/w), respectively. The developed HPLC method was appropriate and practical for the simultaneous analysis of crypto-chlorogenic acid, isoquercetin and astragalin in the leaf extract of M. oleifera. This work is valuable as guidance for the standardization of the leaf extracts and pharmaceutical products of M. oleifera. PMID:23828911

Vongsak, Boonyadist; Sithisarn, Pongtip; Gritsanapan, Wandee

2014-08-01

211

Gene Expression Rhythms in the Mussel Mytilus galloprovincialis (Lam.) across an Annual Cycle  

PubMed Central

Seasonal environmental changes may affect the physiology of Mytilus galloprovincialis (Lam.), an intertidal filter-feeder bivalve occurring commonly in Mediterranean and Atlantic coastal areas. We investigated seasonal variations in relative transcript abundance of the digestive gland and the mantle (gonads) of males and females. To identify gene expression trends – in terms of relative mRNA abundance- we used a medium-density cDNA microarray (1.7 K probes) in dual-color competitive hybridization analyses. Hierarchical clustering of digestive gland microarray data showed two main branches, distinguishing profiles associated with the “hot” months (May–August) from the other months. Genes involved in chitin metabolism, associated with mussel nutrition and digestion showed higher mRNA levels during summer. Moreover, we found different gene transcriptomic patterns in the digestive glands of males when compared to females, during the four stages of mussel gonadal development. Microarray data from gonadal transcripts also displayed clear patterns during the different developmental phases respect to the resting period (stage I) with peak relative mRNA abundance at the ripe phase (stage III) for both sexes. These data showed a clear temporal pattern in transcriptomic profiles of mussels sampled over an annual cycle. Physiological response to thermal variation, food availability, and reproductive status across months may contribute to variation in relative mRNA abundance. PMID:21573210

Banni, Mohamed; Negri, Alessandro; Mignone, Flavio; Boussetta, Hamadi; Viarengo, Aldo; Dondero, Francesco

2011-01-01

212

Hybridization and invasion: an experimental test with diffuse knapweed (Centaurea diffusa Lam.)  

PubMed Central

A number of studies have suggested a link between hybridization and invasion. In this study, we experimentally test the potential for hybridization to influence invasion through a greenhouse common garden study. Diffuse knapweed (DK) (Centaurea diffusa Lam.) was introduced to North America with admixture from spotted knapweed (SK) (Centaurea stoebe subsp. stoebe L.). Comparisons between North American DK (including hybrid phenotypes) and native (European) DK in a common garden did not reveal enhanced performance or increased phenotypic variance, suggesting that pre-introduction hybridization or, more generally, post-introduction evolutionary change has not significantly contributed to the invasion of DK. In contrast, early generation hybrids [artificially created Backcross 1 (BC1) plants] exhibited increased variance for eight of the examined traits, and greater leaf and reproductive shoot production when compared to North American DK. Individual BC1 lines differed for several traits, suggesting the importance of the cross for drawing conclusions from such comparisons. When compared to the parental species (DK and SK), the BC1 plants were not transgressive for any of the measured traits. Overall, these findings suggest that if diploid SK is introduced to North America, interspecific hybridization has the potential to result in even more aggressive invaders.

Blair, Amy C; Blumenthal, Dana; Hufbauer, Ruth A

2012-01-01

213

Removal of cadmium from aqueous system by shelled Moringa oleifera Lam. seed powder.  

PubMed

The present study explores the unexploited sorption properties of the plant Moringa oleifera Lam. for decontamination of Cd at laboratory scale. Sorption studies using standard practices were carried out in batch experiments as functions of biomass dosage, contact time, metal concentrations, particle size and pH. Percentage sorption in each case was computed on the basis of Cd estimation using a planar NaI (TI) detector coupled to a 4K MCA (Canberra Accuspec Card with PC-AT 386). The adsorption data accurately in a Freundlich isotherm. Sorption studies resulted in the standardization of optimum conditions for removal of Cd (85.10%) as follows: biomass dosage (4.0 g), metal concentration (25 microg/ml), contact time (40 min) and volume of the test solution (200 ml) at pH 6.5. Fourier transform infrared (FTIR) spectrometry highlighted amino acid-Cd interactions responsible for sorption phenomenon. The findings open up new avenues in the removal of toxic metals by shelled Moringa oleifera seeds (SMOS) from water bodies as low cost, domestic and environmentally friendly safe technology. PMID:15949938

Sharma, Parul; Kumari, Pushpa; Srivastava, M M; Srivastava, Shalini

2006-01-01

214

Gene expression rhythms in the mussel Mytilus galloprovincialis (Lam.) across an annual cycle.  

PubMed

Seasonal environmental changes may affect the physiology of Mytilus galloprovincialis (Lam.), an intertidal filter-feeder bivalve occurring commonly in Mediterranean and Atlantic coastal areas. We investigated seasonal variations in relative transcript abundance of the digestive gland and the mantle (gonads) of males and females. To identify gene expression trends - in terms of relative mRNA abundance- we used a medium-density cDNA microarray (1.7 K probes) in dual-color competitive hybridization analyses. Hierarchical clustering of digestive gland microarray data showed two main branches, distinguishing profiles associated with the "hot" months (May-August) from the other months. Genes involved in chitin metabolism, associated with mussel nutrition and digestion showed higher mRNA levels during summer. Moreover, we found different gene transcriptomic patterns in the digestive glands of males when compared to females, during the four stages of mussel gonadal development. Microarray data from gonadal transcripts also displayed clear patterns during the different developmental phases respect to the resting period (stage I) with peak relative mRNA abundance at the ripe phase (stage III) for both sexes. These data showed a clear temporal pattern in transcriptomic profiles of mussels sampled over an annual cycle. Physiological response to thermal variation, food availability, and reproductive status across months may contribute to variation in relative mRNA abundance. PMID:21573210

Banni, Mohamed; Negri, Alessandro; Mignone, Flavio; Boussetta, Hamadi; Viarengo, Aldo; Dondero, Francesco

2011-01-01

215

Phytochemical Analysis and Metal-chelation Activity of Achillea tenuifolia Lam.  

PubMed Central

Achillea tenuifolia Lam. (Asteraceae) afforded a dichloromethane fraction from which three known compounds ?-sitosterol (compound1), 5-hydroxy, 4',6,7– trimethoxy flavone (salvigenin compound 2), and methyl-gallate (compound 3) were isolated for the first time. The structure of isolated compounds was elucidated by different spectroscopic methods. Applying the molar-ratio method, the complexation of salvigenin with Fe (III), Cu(II) and Zn(II), the most abundant type of metal ions in the body, were then evaluated. It was determined that stoichiometric ratio of salvigenin with these cations were as Fe(Salvigenin)2 (H2O)2 and Cu(Salvigenin)2(H2O)2 in methanolic solution without pH control, while zinc ions didn`t form significant complexes. The results were confirmed more, by computational molecular modeling of the structure of proposed ligand-complexes by semi-imperical PM3 calculations, which determined negative heat of formation for the complexes Fe(III) and Cu(II) ions as -689.7 and -573.5, respectively and proposed chelating affinity of salvigenin in the following order: Fe(III) > Cu(II) >> Zn(II). PMID:24250440

Moradkhani, Shirin; Ayatollahi, Abdul Majid; Ghanadian, Mustafa; Moin, Mohammad Reza; Razavizadeh, Masoud; Shahlaei, Mohsen

2012-01-01

216

Anticancer, chemopreventive and radioprotective potential of black plum (Eugenia jambolana lam.).  

PubMed

Despite good understanding of the molecular basis of the disease and advances in treatment, globally cancer is still a major cause of death. Estimates are that it will surpass cardiovascular disease as the leading cause of death, with higher incidences in the developing countries that have minimal resources. Chemotherapy and radiotherapy, the two most commonly used treatment modalities, are associated with untoward side effects. This has necessitated the search for alternatives that are effective, non toxic and easily affordable for patients and traditional medicinal plants are an ideal source. Eugenia jambolana Lam., commonly known as black plum or 'jamun' is an important medicinal plant in various traditional systems of medicine. It is effective in the treatment of diabetes mellitus, inflammation, ulcers and diarrhea and preclinical studies have also shown it to possess antineoplastic, chemopreventive and radioprotective properties. Here, for the first time, the effects of jamun in treatment and prevention of cancer, and the mechanisms responsible for these effects are appraised. Additionally the drawbacks in existing knowledge are also stressed to emphasize the possible avenues that need to be investigated, so that maximum effects on both prevention and cure can be attained. PMID:21517226

Baliga, Manjeshwar Shrinath

2011-01-01

217

Investigation on the influence of foreign metal ions in crystal growth and characterization of L-Alaninium Maleate (LAM) single crystals.  

PubMed

A Nonlinear Optical, good quality, single crystals of doped and undoped l-Alaninium Maleate (LAM) were grown by slow evaporation solution growth technique at room temperature. The lattice parameters were analyzed by single crystal X-ray diffraction technique. The identification of Cadmium ion in the doped crystals was done using the EDAX spectrum. The presence of functional group of the dopant with LAM molecule was studied using FTIR spectra. The results of UV-Vis study is used to compare the transparencies of the doped and undoped LAM crystals. The optical band gap energy of the grown crystal was also calculated. The relative second harmonic generation (SHG) efficiency measurement with KDP reference is used to find the incorporation of metal to l-Alaninium Maleate crystals and the parent material. Also the thermal stability of the grown crystals was studied by TGA/DTA spectrum. The mechanical stability of the grown crystals was confirmed through Vickers micro hardness study. By parallel plate capacitor technique, the dielectric response was studied over a wide range of frequencies at different temperatures. The various studies showed the incorporation of the impurity Cd(2+) into LAM crystals and the investigations indicated that the impurity played an important role in the changes of the spectral and structural properties of LAM crystals. PMID:23892119

Ruby Nirmala, L; Thomas Joseph Prakash, J

2013-11-01

218

Electrokinetic detection for X-ray spectra of weakly interacting liquids: n-decane and n-Royce K. Lam, Orion Shih, Jacob W. Smith, Alex T. Sheardy, Anthony M. Rizzuto, David Prendergast, and  

E-print Network

Royce K. Lam, Orion Shih, Jacob W. Smith, Alex T. Sheardy, Anthony M. Rizzuto, David Prendergast-nonane Royce K. Lam,1,2 Orion Shih,1 Jacob W. Smith,1,2 Alex T. Sheardy,1,2 Anthony M. Rizzuto,1,2 David

Cohen, Ronald C.

219

Analgesic, neuropharmacological, anti-diarrheal, and cytotoxic activities of the extract of Solanum sisymbriifolium (Lam.) leaves  

PubMed Central

Objective: The present study was undertaken to evaluate the possible analgesic, neuropharmacological, anti-diarrheal, and cytotoxic activities of the ethanol extract of leaves of Solanum sisymbriifolium Lam. (Family: Solanaceae). Materials and Methods: The analgesic activity was measured by acetic acid-induced writhing inhibition test. The neuropharmacological activities were evaluated using hole cross, hole board, and elevated plus-maze test and the anti-diarrheal activity was assessed using castor oil-induced diarrhea inhibition method. Brine shrimp lethality bioassay was carried out for assessing the cytotoxicity of the ethanol extract of the leaves. Except cytotoxic activity, all the tests were conducted on mice. Results: The extract at oral doses of 200 and 400 mg/kg body weight showed highly significant (p<0.001) decrease in number of writhing, 52.1±0.66 and 4.4±0.64 compared with the control (78.6±0.29) with the percentage of inhibitions of writhing response were found to be 33.72% and 94.40%, respectively. Compare with the control, the extract at both doses showed significant sedative effect in hole cross test. In hole board test, the extract exhibited highly significant (p<0.001) anxiolytic activity at dose of (200 mg/kg), while the same activity was observed at dose of 400 mg/kg in elevated plus-maze test. The extract showed highly significant (p<0.001) anti-diarrheal activity in a dose-dependent manner. With the extract, significant lethality to brine shrimp was found with LC50 value of 61.66±0.9 ?g/ml, which was comparable with the positive control (LC50: 11.89±0.8 µg/ml). Conclusion: The results from the present studies support the traditional uses of this plant part and could form the basis of further investigation including compound isolation. PMID:25050287

Apu, Apurba Sarker; Bhuyan, Shakhawat Hossan; Matin, Maima; Hossain, Faruq; Khatun, Farjana; Taiab, Abu; Jamaluddin

2013-01-01

220

Chemical characteristics and fractionation of proteins from Moringa oleifera Lam. leaves.  

PubMed

Moringa oleifera Lam. is a leguminous plant, originally from Asia, which is cultivated in Brazil because of its low production cost. Although some people have used this plant as food, there is little information about its chemical and nutritional characteristics. The objective of this study was to characterise the leaves of M. oleifera in terms of their chemical composition, protein fractions obtained by solubility in different systems and also to assess their nutritional quality and presence of bioactive substances. The whole leaf flour contained 28.7% crude protein, 7.1% fat, 10.9% ashes, 44.4% carbohydrate and 3.0mg 100g(-1) calcium and 103.1mg 100g(-1) iron. The protein profile revealed levels of 3.1% albumin, 0.3% globulins, 2.2% prolamin, 3.5% glutelin and 70.1% insoluble proteins. The hydrolysis of the protein from leaf flour employing sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (ME) resulted in 39.5% and 29.5%, respectively. The total protein showed low in vitro digestibility (31.8%). The antinutritional substances tested were tannins (20.7 mg g(-1)), trypsin inhibitor (1.45TIU mg g(-1)), nitrate (17 mg g(-1)) and oxalic acid (10.5 mg g(-1)), besides the absence of cyanogenic compounds. ?-Carotene and lutein stood out as major carotenoids, with concentrations of 161.0 and 47.0 ?g g(-1) leaf, respectively. Although M. oleifera leaves contain considerable amount of crude protein, this is mostly insoluble and has low in vitro digestibility, even after heat treatment and chemical attack. In vivo studies are needed to better assess the use of this leaf as a protein source in human feed. PMID:24206684

Teixeira, Estelamar Maria Borges; Carvalho, Maria Regina Barbieri; Neves, Valdir Augusto; Silva, Maraíza Apareci; Arantes-Pereira, Lucas

2014-03-15

221

Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity  

PubMed Central

The present study was designed to investigate the antioxidant activity of aqueous and methanol extracts of Erythrina indica Lam leaves by in vitro methods viz. 1, 1-Diphenyl-2-Picrylhydrazyl, nitric oxide radical scavenging activity, and inhibition of lipid peroxidation by thiobarbituric acid reactive substances (TBARS) method on isolated rat liver tissues. Quantitative analysis of antioxidative components like total amount of phenolics, flavonoids, and flavonols were estimated using the spectrophotometric method. Linear regression analysis was used to calculate the IC50 value. Results showed that the aqueous and methanol extracts exhibited significant DPPH radicals scavenging activity with an IC50 value 342.59 ± 19.59, 283.24 ± 12.28 µg/mL respectively. Nitric oxide radicals were significantly scavenged by the aqueous and methanol extracts (IC50 = 250.12 ± 10.66; 328.29 ± 3.74 µg/mL). Lipid peroxidation induced by the Fe2+ was inhibited by the aqueous extract with low IC50 value (97.29 ± 2.05 µg/mL) as compared to methanol extract (IC50 = 283.74 ± 5.70 µg/mL). Both the extracts were exhibited similar quantities of total phenolics. Total flavonoids were found to be in higher quantities than total flavonols in aqueous extract as compared to methanol extract. From the results, it is concluded that the aqueous and methanol extracts of E. indica leaves possesses significant antioxidant activity that may be due to the presence of flavonoids and related polyphenolic compounds. PMID:21331194

Sakat, SS; Juvekar, AR

2010-01-01

222

Anti-nutritional factors in the roots of a local cultivar of Moringa oleifera (Lam).  

PubMed

The evergreen plant, Moringa oleifera (Lam) has been known to have both medicinal and nutritional properties, thus its wide use in traditional medicine in Africa and Asia. The roots, in particular, have been reported to possess antibiotic, anti-tumour and anti-oxidative activities. This study therefore seeks to determine the levels of the anti-nutritional factors and other proximate analyses in the roots of a local cultivar of Moringa oleifera which might be responsible for such activities. The concentrations of oxalates and phytates in the roots were determined using the methods of Munro and Bassir and Griffith and Thomas, respectively. The methods of Association of Analytical Chemists (AOAC) were used to estimate the amount of tannins, saponins and cyanogenic glycosides while the Technicon sequential Multi-sample amino acid analyzer (TSM) was used determine the amino acid concentration in the roots. Tannins (45 mg 100 g(-1)) and oxalates (17.08 mg 100 g(-1)) were present in the roots at higher levels while saponins (4.20mg 100 g(-1)), cyanogenic glycosides (2.72 mg 100 g(-1)) and phytates (0.07 mg 100 g(-1)) occurred at much lower levels. The roots contained (Mean +/- Standard Error of mean) %crude lipid (6.33 +/- 1.64), %crude proteins (5.02 +/- 1.52), %carbohydrates (76.75), %ash (4.97 +/- 0.53) and %moisture (6.93 +/- 0.58). The roots lacked the water-soluble vitamins pyridoxine, riboflavin and thiamine but contained ascorbic acid (48.13 mg 100 g(-1)) and niacin (5.83 mg 100 g(-1)). This study has shown that Moringa oleifera roots are rich in anti-nutritional factors and that is why they are widely used in traditional medicine in Africa, Asia and Americas for its medicinal importance. PMID:24783788

Igwilo, I O; Ezeonu, F C; Ezekwesili-Ofili, J O; Igwilo, S N; Nsofor, C I; Abdulsalami, M S; Obi, E

2014-01-01

223

Transcriptional Response of the Mussel Mytilus galloprovincialis (Lam.) following Exposure to Heat Stress and Copper  

PubMed Central

Global warming is a major factor that may affect biological organization, especially in marine ecosystems and in coastal areas that are particularly subject to anthropogenic pollution. We evaluated the effects of simultaneous changes in temperature and copper concentrations on lysosomal membrane stability (N-acetyl-hexosaminidase activity) and malondialdehyde accumulation (MDA) in the gill of the blue mussel Mytilus galloprovincialis (Lam.). Temperature and copper exerted additive effects on lysosomal membrane stability, exacerbating the toxic effects of metal cations present in non-physiological concentrations. Mussel lysosomal membrane stability is known to be positively related to scope for growth, indicating possible effects of increasing temperature on mussel populations in metal-polluted areas. To clarify the molecular response to environmental stressors, we used a cDNA microarray with 1,673 sequences to measure the relative transcript abundances in the gills of mussels exposed to copper (40 µg/L) and a temperature gradient (16°C, 20°C, and 24°C). In animals exposed only to heat stress, hierarchical clustering of the microarray data revealed three main clusters, which were largely dominated by down-regulation of translation-related differentially expressed genes, drastic up-regulation of protein folding related genes, and genes involved in chitin metabolism. The response of mussels exposed to copper at 24°C was characterized by an opposite pattern of the genes involved in translation, most of which were up-regulated, as well as the down-regulation of genes encoding heat shock proteins and “microtubule-based movement” proteins. Our data provide novel information on the transcriptomic modulations in mussels facing temperature increases and high copper concentrations; these data highlight the risk of marine life exposed to toxic chemicals in the presence of temperature increases due to climate change. PMID:23825565

Negri, Alessandro; Oliveri, Catherina; Sforzini, Susanna; Mignione, Flavio; Viarengo, Aldo; Banni, Mohamed

2013-01-01

224

In vitro antibacterial potency of Butea monosperma Lam. against 12 clinically isolated multidrug resistant bacteria  

PubMed Central

Objective To investigate the antibacterial activity, using cold and hot extraction procedures with five solvents, petroleum ether, acetone, ethanol, methanol and water to validate medicinal uses of Butea monosperma Lam (B. monosperma) in controlling infections; and to qualitatively estimate phytochemical constituents of leaf-extracts of the plant. Methods The antibacterial activity of leaf-extracts was evaluated by the agar-well diffusion method against clinically isolated 12 Gram-positive and -negative multidrug resistant (MDR) pathogenic bacteria in vitro. Values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of leaf-extracts against each bacterium were obtained in a 96-well micro-titre plate, by broth dilution micro-titre plate technique. Results The presence of tannins, flavonoids, starch, glycosides and carbohydrates in different leaf extracts was established. Pathogenic bacteria used were, Acinetobacter sp., Chromobacterium violaceum, Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Shigella sp., Enterococcus sp., Staphylococcus aureus (S. aureus), methicillin resistant S. aureus and vancomycin resistant S. aureus, along with standard bacterial strains. These MDR bacteria had been recorded to have significant inhibitions by leaf extracts, obtained by cold and hot extraction procedures with five solvents. In addition, the hot aqueous extract against Enterococcus sp. had the highest inhibition zone-size (21 mm). Ciprofloxacin 30 µg/disc was the positive/reference control and the diluting solvent, 10% dimethyl sulphoxide was the negative control. Recorded MIC values of different extracts ranged between 0.23 and 13.30 mg/mL, and MBC values were 0.52 to 30.00 mg/mL, for these bacteria. Conclusions Leaf-extracts with hot water and ethanol had shown significant antibacterial activity against all bacteria. B. monosperma leaf-extract could be used in treating infectious diseases, caused by the range of tested bacteria, as complementary and alternate medicine.

Sahu, Mahesh Chandra; Padhy, Rabindra Nath

2013-01-01

225

Oxygen consumption rate and Na+/K+-ATPase activity in early developmental stages of the sea urchin Paracentrotus lividus Lam.  

NASA Astrophysics Data System (ADS)

Changes in oxygen consumption rate and Na+/K+-ATPase activity during early development were studied in the sea urchin Paracentrotus lividus Lam. The oxygen consumption rate increased from 0.12 ?mol O2 mg protein-1 h-1 in unfertilized eggs to 0.38 ?mol O2 mg protein-1 h-1 25 min after fertilization. Specific activity of the Na+/K+-ATPase was significantly stimulated after fertilization, ranging up to 1.07 ?mol Pi h-1 mg protein-1 in the late blastula stage and slightly lower values in the early and late pluteus stages.

Tomši?, Sanja; Stankovi?, Suzana; Lucu, ?edomil

2011-09-01

226

Performance Comparison of a Matrix Solver on a Heterogeneous Network Using Two Implementations of MPI: MPICH and LAM  

NASA Technical Reports Server (NTRS)

Two of the current and most popular implementations of the Message-Passing Standard, Message Passing Interface (MPI), were contrasted: MPICH by Argonne National Laboratory, and LAM by the Ohio Supercomputer Center at Ohio State University. A parallel skyline matrix solver was adapted to be run in a heterogeneous environment using MPI. The Message-Passing Interface Forum was held in May 1994 which lead to a specification of library functions that implement the message-passing model of parallel communication. LAM, which creates it's own environment, is more robust in a highly heterogeneous network. MPICH uses the environment native to the machine architecture. While neither of these free-ware implementations provides the performance of native message-passing or vendor's implementations, MPICH begins to approach that performance on the SP-2. The machines used in this study were: IBM RS6000, 3 Sun4, SGI, and the IBM SP-2. Each machine is unique and a few machines required specific modifications during the installation. When installed correctly, both implementations worked well with only minor problems.

Phillips, Jennifer K.

1995-01-01

227

Target site mutation and reduced translocation are present in a glyphosate-resistant Lolium multiflorum Lam. biotype from Spain.  

PubMed

The resistance mechanism of a glyphosate-resistant Lolium multiflorum Lam. biotype collected in Córdoba (Southern Spain) was examined. Resistance Factor values at three different growth stages ranged between 4.77 and 4.91. At 96 hours after treatment (HAT) the S biotype had accumulated seven times more shikimic acid than the R biotype. There were significant differences in translocation of (14)C-glyphosate between biotypes, i.e. at 96 HAT, the R biotype accumulated in the treated leaf more than 70% of the absorbed herbicide, in comparison with 59.21% of the S biotype; the R biotype translocated only 14.79% of the absorbed (14)C-glyphosate to roots, while in the S population this value was 24.79%. Visualization of (14)C-glyphosate by phosphor imaging showed a reduced distribution in the R biotype compared with the S. Glyphosate metabolism was not involved in the resistance mechanism due to both biotypes showing similar values of glyphosate at 96 HAT. Comparison of the EPSPS gene sequences between biotypes indicated that the R biotype has a proline 182 to serine amino acid substitution. In short, the resistance mechanism of the L. multiflorum Lam. biotype is due to an impaired translocation of the herbicide and an altered target site. PMID:22771431

González-Torralva, Fidel; Gil-Humanes, Javier; Barro, Francisco; Brants, Ivo; De Prado, Rafael

2012-09-01

228

The ?-Glucanase ZgLamA from Zobellia galactanivorans Evolved a Bent Active Site Adapted for Efficient Degradation of Algal Laminarin*  

PubMed Central

Laminarinase is commonly used to describe ?-1,3-glucanases widespread throughout Archaea, bacteria, and several eukaryotic lineages. Some ?-1,3-glucanases have already been structurally and biochemically characterized, but very few from organisms that are in contact with genuine laminarin, the storage polysaccharide of brown algae. Here we report the heterologous expression and subsequent biochemical and structural characterization of ZgLamAGH16 from Zobellia galactanivorans, the first GH16 laminarinase from a marine bacterium associated with seaweeds. ZgLamAGH16 contains a unique additional loop, compared with other GH16 laminarinases, which is composed of 17 amino acids and gives a bent shape to the active site cleft of the enzyme. This particular topology is perfectly adapted to the U-shaped conformation of laminarin chains in solution and thus explains the predominant specificity of ZgLamAGH16 for this substrate. The three-dimensional structure of the enzyme and two enzyme-substrate complexes, one with laminaritetraose and the other with a trisaccharide of 1,3–1,4-?-d-glucan, have been determined at 1.5, 1.35, and 1.13 ? resolution, respectively. The structural comparison of substrate recognition pattern between these complexes allows the proposition that ZgLamAGH16 likely diverged from an ancestral broad specificity GH16 ?-glucanase and evolved toward a bent active site topology adapted to efficient degradation of algal laminarin. PMID:24337571

Labourel, Aurore; Jam, Murielle; Jeudy, Alexandra; Hehemann, Jan-Hendrik; Czjzek, Mirjam; Michel, Gurvan

2014-01-01

229

Do Ecological Niche Model Predictions Reflect the Adaptive Landscape of Species?: A Test Using Myristica malabarica Lam., an Endemic Tree in the Western Ghats, India  

E-print Network

Bioclim (DIVA GIS version 7.3) and Maxent (version 3.3.2) to predict the habitat suitability of Myristica malabarica Lam., an economically important tree occurring in the Western Ghats, India. We located populations of the trees naturally occurring...

Nagaraju, Shivaprakash K.; Gudasalamani, Ravikanth; Barve, Narayani; Ghazoul, Jaboury; Narayangowda, Ganeshaiah Kotiganahalli; Ramanan, Uma Shaanker

2013-11-29

230

CARACTERÍSTICAS DA CARCAÇA DE CORDEIROS MANTIDOS EM PASTO DE AZEVÉM COMUM (Lolium multiflorum Lam), EM PASTEJO CONTÍNUO, SUBMETIDOS A DIFERENTES NÍVEIS DE ADUBAÇÃO NITROGENADA 1  

Microsoft Academic Search

The experiment evaluated the effect of the levels of nitrogen fertilization, of italian ryegrass (Lolium multiflorum Lam.), on the characteristics of carcass of lambs kept in continuous stocking. There where four doses of nitrogen (N), using commercial urea (45-00-00), with only application: T1 - level 0 of N \\/hectare (ha); T2 - 75 kg of N \\/ha; T3 - 150

Luiz Giovani de Pellegrini; Alda Lúcia Gomes Monteiro; Mikael Neumann; Aníbal de Moraes; Amadeu Bona Filho; Luciane Rodrigues Cardoso

231

Mechanism of Supported Membrane Disruption by Antimicrobial Peptide Protegrin-1 Kin Lok H. Lam,,# Yuji Ishitsuka,,# Yishan Cheng, Karen Chien, Alan J. Waring,|,  

E-print Network

Mechanism of Supported Membrane Disruption by Antimicrobial Peptide Protegrin-1 Kin Lok H. Lam disruption by antimicrobial peptide protegrin-1 (PG-1) on dimyristoyl-sn-glycero- phosphocholine been suggested as an important step in the membrane disruption process induced by antimicrobial

Lee, Ka Yee C.

232

In vivo anticoccidial activity of berberine [18, 5,6-dihydro-9,10-dimethoxybenzo(g)-1,3-benzodioxolo(5,6-a) quinolizinium]--an isoquinoline alkaloid present in the root bark of Berberis lycium.  

PubMed

Coccidiosis, caused by various Eimeria species, is a major parasitic disease in chicken. However the increasing resistance of these parasites to currently used anticoccidial drugs has stimulated the search for new methods of control. As part of this effort we investigated the root bark of Berberis lycium (barberry) as a potential source of compounds with anticoccidial activity. In the present study anticoccidial activity of different solvent extracts of the root bark of B. lycium and berberine was evaluated in vivo using broiler chicken. Results of the study demonstrated equipotent efficacy of pure berberine in comparison to that of standard drug amprolium on the basis of reduction in coccidian oocyst output, body weight gain of chicken and feed conversion ratio. Among the extracts crude methanolic extract showed highest anticoccidial activity tested at 300 mg/kg body weight which could be due to the presence of alcohol-soluble active ingredients in root bark of B. lycium. Toxicological studies revealed that B. lycium extracts as well as berberine were not lethal up to dosage of 2000 mg/kg body weight. LD(50) was not determined as mortalities were not recorded in any of the five groups of chicken. From the present study it can be concluded that root bark of B. lycium has the immense potential to contribute to the control of coccidian parasites of chicken. Our results corroborate the use of berberine for treatment of severe diarrhoea, amoebiasis and intestinal infections and could justify its use in folk medicine for treatment of haemorrhagic dysentery. PMID:24411651

Malik, Tauseef Ahmad; Kamili, Azra N; Chishti, M Z; Tanveer, Syed; Ahad, Shazia; Johri, R K

2014-04-15

233

Genetic diversity and relationships in cultivars of Lolium multiflorum Lam. using sequence-related amplified polymorphism markers.  

PubMed

Sequence-related amplified polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multiflorum Lam.). Eighteen SRAP primer combinations generated 334 amplification bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1) to 0.5000 (me5 + em7), with an average of 0.4921. The genetic similarity coefficient ranged from 0.4304 to 0.8529, and coefficients between 0.65 and 0.90 accounted for 90.00%. The cluster analysis separated the accessions into five groups partly according to their germplasm resource origins. PMID:25501225

Huang, L K; Jiang, X Y; Huang, Q T; Xiao, Y F; Chen, Z H; Zhang, X Q; Miao, J M; Yan, H D

2014-01-01

234

Studies of Pb2+ adsorption by Moringa oleifera Lam. seeds from an aqueous medium in a batch system.  

PubMed

The efficiency of Moringa (Moringa oleifera Lam.) seeds for removing lead ions (Pb(2+)) from water was evaluated. Parameters such as solution pH, adsorbent mass, contact time between solution and adsorbent, isotherms, thermodynamic, kinetics, and desorption were evaluated. The maximum adsorption capacity of the biosorbent was found to be 12.24 mg g(-1). In order to verify the effectiveness of this material, comparative studies were performed with activated carbon under the same optimal conditions for the construction of isotherms and the desorption process. Average desorption rate values led to the assumption that a strong interaction took place between the adsorbents and the metal ions. Thus, it has been concluded that the biosorbent studied herein can be considered very effective and feasible for remediating Pb(2+)-contaminated solutions, since this material is itself an untreated and low-cost byproduct. PMID:24434983

Meneghel, Ana Paula; Gonçalves, Affonso Celso; Tarley, César Ricardo Teixeira; Stangarlin, José Renato; Rubio, Fernanda; Nacke, Herbert

2014-01-01

235

Biochemical and physiological alterations in female reproductive organs of cyclic rats treated with aqueous extract of Moringa oleifera Lam.  

PubMed

Biochemical and physiological alterations have been observed in the genital tract of female cyclic rats treated with aqueous extract of M. oleifera Lam. Its administration caused a significant increase in the glycogen contents, protein concentration, activity of acid and alkaline phosphatase and the level of total cholesterol in all the organs at initial days of treatment. However, at longer days of treatment the values revealed a significant depletion. Initially its administration stimulated the uterine structures, caused metaplastic changes in the cervical epithelium and provoked considerable cornification in the vaginal epithelium. At later durations significant inhibition in the histoarchitecture was observed. Biochemical observations supplemented with the histological findings have been correlated with the anti-implantation action of the aqueous extract in the light of its hormonal properties. PMID:3227756

Shukla, S; Mathur, R; Prakash, A O

1988-01-01

236

Suppressive effects of Moringa oleifera Lam pod against mouse colon carcinogenesis induced by azoxymethane and dextran sodium sulfate.  

PubMed

Moringa oleifera Lam (horseradish tree; tender pod or fruits) is a major ingredient in Thai cuisine and has some medicinal properties. Previous studies have shown potentially antioxidant, antitumor promoter, anticlastogen and anticarcinogen activities both in vitro and in vivo. The present study was conducted to investigate chemopreventive effects on azoxymethane (AOM)-initiated and dextran sodium sulfate (DSS)-promoted colon carcinogenesis in mice. Male ICR mice were divided into 8 groups: Group 1 served as a negative control; Group 2 received AOM/DSS as a positive control; Groups 3-5 were fed boiled freeze-dried M. oleifera (bMO) at 1.5%, 3.0% and 6.0%, respectively supplemented in basal diets for 5 weeks; Groups 6-8 were fed with bMO diets at the designed doses above for 2 weeks prior to AOM, during and 1 week after DSS administration. At the end of the study, colon samples were processed for histopathological examination. PCNA indices, and iNOS and COX-2 expression were assessed by immunohistochemistry. The results demonstrated the incidences and multiplicities of tumors in Groups 6-8 to be decreased when compared to Group 2 in a dose dependent manner, but this was significant only in Group 8. The PCNA index was also significantly decreased in Group 8 whereas iNOS and COX-2 protein expression were significantly decreased in Groups 7 and 8. The findings suggest that M. oleifera Lam pod exerts suppressive effects in a colitis-related colon carcinogenesis model induced by AOM/DSS and could serve as a chemopreventive agent. PMID:22471457

Budda, Sirintip; Butryee, Chaniphun; Tuntipopipat, Siriporn; Rungsipipat, Anudep; Wangnaithum, Supradit; Lee, Jeong-Sang; Kupradinun, Piengchai

2011-01-01

237

Adsorption of bacteriophage lambda on the LamB protein of Escherichia coli K-12: point mutations in gene J of lambda responsible for extended host range.  

PubMed Central

LamB is the cell surface receptor for bacteriophage lambda. LamB missense mutations yielding resistance to lambda group in two classes. Class I mutants block the growth of lambda with the wild-type host range (lambda h+) but support the growth of one-step host range mutants (lambda h). Class II mutants block lambda h but support the growth of two-step host range mutant (lambda hh*) phages. To identify amino acid residues in the J protein (the tail fiber of phage lambda) responsible for the extended host range phenotype of mutants of phage (lambda h+), we selected a series of one-step (lambda h) and two-step (lambda hh*) host range mutants and analyzed their corresponding J genes. Three different class I LamB missense mutants (mutations at sites 247, 245, and 148) were used to select 11 independent, new, one-step host range mutants (lambda h phages). DNA sequence analysis revealed a single-amino-acid change in each case. The 11 alterations affected only three residues in the distal part of J, corresponding to a Val-->Ala change at site 1077 in five cases, a Thr-->Met change at site 1040 in three cases, and a Leu-->Pro change at site 1127 in three cases. Recombination experiments confirmed that in the cases tested, the mutations identified were indeed responsible for the extended host range phenotype. The class II LamB mutant (Gly-->Asp at site 151) was used to select two-step extended host range mutants (lambda hh* phages) from three new lambda h phages, corresponding to different amino acid modifications in the J protein (at sites 1040, 1077, and 1127). The new lambda hh* phages analyzed corresponded to either double or triple point mutations located at the distal end of the J protein. In all, seven residues involved in the extended host range properties of lambda mutants were identified in the distal part of the J protein, suggesting that the last C-terminal portion of the J protein participates directly in the adsorption of the phage onto LamB. In agreement with the fact that the lambda h mutants (and the lambda hh* mutants) could grow on all of the lamB class I mutations tested, we found tha the nature of the J mutations did not depend on the LamB class I mutant used to select them. This is interpreted as meaning that the mutated residues in the J protein and in the LamB mutants are not involved in allele-specific protein-protein interactions. Rather, the LamB mutations would block a step in phage adsorption, and this block would be overcome by the mutations in the J protein. PMID:8106335

Werts, C; Michel, V; Hofnung, M; Charbit, A

1994-01-01

238

Adsorption of bacteriophage lambda on the LamB protein of Escherichia coli K-12: point mutations in gene J of lambda responsible for extended host range.  

PubMed

LamB is the cell surface receptor for bacteriophage lambda. LamB missense mutations yielding resistance to lambda group in two classes. Class I mutants block the growth of lambda with the wild-type host range (lambda h+) but support the growth of one-step host range mutants (lambda h). Class II mutants block lambda h but support the growth of two-step host range mutant (lambda hh*) phages. To identify amino acid residues in the J protein (the tail fiber of phage lambda) responsible for the extended host range phenotype of mutants of phage (lambda h+), we selected a series of one-step (lambda h) and two-step (lambda hh*) host range mutants and analyzed their corresponding J genes. Three different class I LamB missense mutants (mutations at sites 247, 245, and 148) were used to select 11 independent, new, one-step host range mutants (lambda h phages). DNA sequence analysis revealed a single-amino-acid change in each case. The 11 alterations affected only three residues in the distal part of J, corresponding to a Val-->Ala change at site 1077 in five cases, a Thr-->Met change at site 1040 in three cases, and a Leu-->Pro change at site 1127 in three cases. Recombination experiments confirmed that in the cases tested, the mutations identified were indeed responsible for the extended host range phenotype. The class II LamB mutant (Gly-->Asp at site 151) was used to select two-step extended host range mutants (lambda hh* phages) from three new lambda h phages, corresponding to different amino acid modifications in the J protein (at sites 1040, 1077, and 1127). The new lambda hh* phages analyzed corresponded to either double or triple point mutations located at the distal end of the J protein. In all, seven residues involved in the extended host range properties of lambda mutants were identified in the distal part of the J protein, suggesting that the last C-terminal portion of the J protein participates directly in the adsorption of the phage onto LamB. In agreement with the fact that the lambda h mutants (and the lambda hh* mutants) could grow on all of the lamB class I mutations tested, we found tha the nature of the J mutations did not depend on the LamB class I mutant used to select them. This is interpreted as meaning that the mutated residues in the J protein and in the LamB mutants are not involved in allele-specific protein-protein interactions. Rather, the LamB mutations would block a step in phage adsorption, and this block would be overcome by the mutations in the J protein. PMID:8106335

Werts, C; Michel, V; Hofnung, M; Charbit, A

1994-02-01

239

The Effects of Berberis vulgaris Fruit Extract on Serum Lipoproteins, apoB, apoA-I, Homocysteine, Glycemic Control and Total Antioxidant Capacity in Type 2 Diabetic Patients.  

PubMed

Type 2 diabetes is a well-known endocrine and metabolic disorder which has reached epidemic proportions worldwide and represents a serious public health concern. Hyperglycemia and dyslipidemia are two major abnormalities which are major cardiovascular risk factors. Berberine is a major alkaloid in Berberis vulgaris fruit extract (BVFE) which have important role in regulation of serum glucose and fat metabolism in-vivo and in-vitro but its role in type 2 diabetes have not been extensively examined. The aim of this study was the effect of BVFE on serum lipoproteins, apoB, apoA-I, homocysteine, glycemic control and total antioxidant capacity in type 2 diabetic patients. In a double-blind randomised clinical trial, 31 diabetic patients were randomly assigned to 3 g/d BVFE or placebo for 3 months. Serum glucose, lipoproteins, apoB, apoA-I, insulin, homocysteine and HbA1c were measured at the baseline and also at the end of the 3(rd) month. At the beginning and end of 1(st), 2(nd) and 3(rd) months, a 24-h dietary recall questionnaire about each patients was completed. Data were analyzed by SPSS version 16. There were significant decreases in serum TG, TC, LDL-c, apo B, glucose, and insulin and also a significant increase in TAC at the end of the study in BVFE group compared to the control group (p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.002, p = 0.01 and p = 0.0001 respectively). There were significant differences in serum TG (p = 0.0001), TC (p = 0.001), LDL-c (p = 0.001), apoB (p = 0.001), glucose (p = 0.002), insulin (p = 0.01), TAC (p = 0.005), and insulin resistance (p = 0.01) between the two groups at the end of the study; but homocysteine, HbA1c and HDL-c showed no significant changes between the two groups at the end of study. The intake of 3 g/d BVFE for 3 months may have benefical effects on lipoproteins, apoproteins, glycemic control and TAC in type 2 diabetic patients. PMID:24250489

Shidfar, Farzad; Ebrahimi, Shima Seyyed; Hosseini, Sharieh; Heydari, Iraj; Shidfar, Shahrzad; Hajhassani, Giti

2012-01-01

240

The Effects of Berberis vulgaris Fruit Extract on Serum Lipoproteins, apoB, apoA-I, Homocysteine, Glycemic Control and Total Antioxidant Capacity in Type 2 Diabetic Patients  

PubMed Central

Type 2 diabetes is a well-known endocrine and metabolic disorder which has reached epidemic proportions worldwide and represents a serious public health concern. Hyperglycemia and dyslipidemia are two major abnormalities which are major cardiovascular risk factors. Berberine is a major alkaloid in Berberis vulgaris fruit extract (BVFE) which have important role in regulation of serum glucose and fat metabolism in-vivo and in-vitro but its role in type 2 diabetes have not been extensively examined. The aim of this study was the effect of BVFE on serum lipoproteins, apoB, apoA-I, homocysteine, glycemic control and total antioxidant capacity in type 2 diabetic patients. In a double-blind randomised clinical trial, 31 diabetic patients were randomly assigned to 3 g/d BVFE or placebo for 3 months. Serum glucose, lipoproteins, apoB, apoA-I, insulin, homocysteine and HbA1c were measured at the baseline and also at the end of the 3rd month. At the beginning and end of 1st, 2nd and 3rd months, a 24-h dietary recall questionnaire about each patients was completed. Data were analyzed by SPSS version 16. There were significant decreases in serum TG, TC, LDL-c, apo B, glucose, and insulin and also a significant increase in TAC at the end of the study in BVFE group compared to the control group (p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.002, p = 0.01 and p = 0.0001 respectively). There were significant differences in serum TG (p = 0.0001), TC (p = 0.001), LDL-c (p = 0.001), apoB (p = 0.001), glucose (p = 0.002), insulin (p = 0.01), TAC (p = 0.005), and insulin resistance (p = 0.01) between the two groups at the end of the study; but homocysteine, HbA1c and HDL-c showed no significant changes between the two groups at the end of study. The intake of 3 g/d BVFE for 3 months may have benefical effects on lipoproteins, apoproteins, glycemic control and TAC in type 2 diabetic patients. PMID:24250489

Shidfar, Farzad; Ebrahimi, Shima Seyyed; Hosseini, Sharieh; Heydari, Iraj; Shidfar, Shahrzad; Hajhassani, Giti

2012-01-01

241

Inhibitory Effect of n-butanol Fraction of Moringa oleifera Lam. Seeds on Ovalbumin-Induced Airway Inflammation in a Guinea Pig Model of Asthma  

Microsoft Academic Search

Moringaceae, which belongs to the Moringa oleifera Lam. family, is a well-known herb used in Asian medicine as an antiallergic drug. In the present study, the efficacy of the n-butanol extract of the seeds of the plant (MONB) is examined against ovalbumin-induced airway inflammation in guinea pigs. The test drugs (MONB or dexamethasone) are administered orally prior to challenge with

Shailaja G. Mahajan; Aryamitra Banerjee; Bhupendrasinh F. Chauhan; Harish Padh; Manish Nivsarkar; Anita A. Mehta

2009-01-01

242

Comparison of minimum inhibitory concentration of water soluble extracts of eugenia jambolana lam. (Fam. Myrtaceae) barks of different ages on dysentery and diarrhoea forming micro - organisms.  

PubMed

A preliminary investigations was carried out to study the antibacterial activity of the water soluble extracts of five and ten years old barks of Eugenia Jambolana Lam. (fam. Myrtaceae) on dysentery and diarrhoea forming micro organisms. It was observed that the barks of young plants have a better inhibitory effect on micro - organisms like Salmonella viballerup, Shigella dysenteriae 10, Shigella boydii 5, Sgigella dysenteriae 2. PMID:22557509

Maiti, A P; Pal, S C; Chattopadhyay, D; De, S; Nandy, A

1985-10-01

243

Preface: Proceedings of the 13th Conference on Liquid and Amorphous Metals (LAM13) (Ekaterinburg, Russia, 8 14 July 2007)  

NASA Astrophysics Data System (ADS)

The most recent developments in the field of liquid and amorphous metals and alloys are regularly updated through two complementary international conferences: the liquid and amorphous metals conference (LAM) and the rapidly quenched materials (RQ) conference. The first series of conferences started as LM1 in 1966 at Brookhaven for the basic understanding of liquid metals. The subsequent LM conferences were held in Tokyo (1972) and Bristol (1976). The conference was renewed in Grenoble (1980) as a LAM conference including amorphous metals and continued in Los Angeles (1983), Garmisch-Partenkirchen (1986), Kyoto (1989), Vienna (1992), Chicago (1995), Dortmund (1998), Yokohama (2001) and Metz (2004). The conferences are mainly devoted to liquid and amorphous metals and alloys. However, communications on some non-metallic systems such as semiconductors, quasicrystals etc, are also accepted. The conference tradition strongly encourages participation from junior researchers and graduate students. The 13th conference of the LAM series was organized in Ekaterinburg, Russia, by the Institute of Metallurgy of the Ural Branch of the Russian Academy of Sciences (IMet UB RAS) and the Ural State Pedagogical University (USPU), and held from 8-14 July 2007 under the chairmanship of Professors Pjotr Popel (USPU) and Boris Gelchinskii (IMet UB RAS). Two hundred and forty two active participants and about 60 guest participants from 20 countries attended the conference. There were no parallel sessions and all oral reports were separated into three groups: invited talks (40 min), full-scale oral reports (25 min), and brief oral reports (15 min). The program included ten sessions, ranging from purely theoretical subjects to the technological application of molten and amorphous alloys. The following sessions took place: A: Electronic structure and transport, magnetic properties; B: Phase transitions; C: Structure; D: Atomic dynamics and transport; E: Thermodynamics; F: Modelling, simulation; G: Surface and interface; H: Mechanical properties and new materials; I: Quasicrystals; J: Industrial applications. The largest sessions were E, C, D and F with 53, 47, 43 and 40 reports, respectively. Posters were exhibited during the first (sessions A-E) or last (sessions F-J) three days of the conference. As usual, each oral session started with an invited talk. In accordance with contemporary tendencies the first invited talk of M Yao (Kyoto University) was devoted to interrelations between liquid metal studies and nanoscience. He pointed out that the physics of disordered matter, especially liquid metals, has provided nanoscience with basic ideas and theoretical tools such as ab initio molecular dynamics (MD) simulation. K Tamura (Kyoto University) reported experimental investigation results of expanded liquid rubidium, showing that observed structural features are originated from the instability of low-density electron gas. Modern theory has predicted that interacting electron gas suffers negative compressibility and the static dielectric function (DF) becomes negative when electron density is sufficiently reduced. Negative DF is of special interest when considering the possibility of new types of superconductors. In the next invited lecture, M I Mendelev and J R Morris (USA) described how MD simulations were performed to study phase transformations in supercooled liquid Al. The authors found that the widely used EAM potential for Al enables vitrification for cooling rates achievable in classical MD simulation, while other Al potentials enable crystallization under the same conditions. The reason for this difference was discussed, as well as features of vitrification in the EA Al. P Häussler (Chemnitz Univeristy) spoke about fundamental structure-forming processes in liquid and amorphous materials. He proposed the resonance model as an autonomous missing link between the microscopic description of atoms/molecules of Schrödinger's equation, and crystals, where global concepts such as planar resonances exist and Bloch's theorem is applied. D Holland-

Popel, Pjotr; Gelchinskii, Boris; Sidorov, Valeriy

2008-03-01

244

Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats.  

PubMed

The leaves of Moringa oleifera Lam (Moringaceae) are used by the Indians in their herbal medicine as a hypocholesterolemic agent in obese patients. The scientific basis for their use in hypercholesterolemia was therefore examined. It was found that administration of the crude leaf extract of Moringa oleifera along with high-fat diet decreased the high-fat diet-induced increases in serum, liver, and kidney cholesterol levels by 14.35% (115-103.2 mg/100 ml of serum), 6.40% (9.4-8.8 mg/g wet weight) and 11.09% (1.09-0.97 mg/g wet weight) respectively. The effect on the serum cholesterol was statistically significant. No significant effect on serum total protein was observed. However, the crude extract increased serum albumin by 15.22% (46-53 g/l). This value was also found to be statistically significant. It was concluded that the leaves of Moringa oleifera have definite hypocholesterolemic activity and that there is valid pharmacological basis for employing them for this purpose in India. PMID:10661880

Ghasi, S; Nwobodo, E; Ofili, J O

2000-01-01

245

Total Phenolics and Total Flavonoids Contents and Hypnotic Effect in Mice of Ziziphus mauritiana Lam. Seed Extract.  

PubMed

The seeds of Ziziphus mauritiana Lam. have been traditionally used for treatment of various complications including insomnia and anxiety. They are popularly used as sedative and hypnotic drugs in China, Korea, Myanmar, Vietnam, and other Asian countries. However, no scientific proof on hypnotic activity of Z. mauritiana seeds (ZMS) was reported. In this study, the hypnotic activity of 50% ethanolic extract from ZMS was observed on the loss of righting reflex in mice using pentobarbital-induced sleep mice method. The contents of total phenolics and total flavonoids in the extract were also determined. The results showed that the 50% ethanolic extract from ZMS contained total phenolics 27.62 ± 1.43?mg gallic acid equivalent (GAE)/g extract and total flavonoids 0.74 ± 0.03?mg quercetin equivalent (QE)/g extract. Oral administration of the extract at the dose of 200?mg/kg significantly increased the sleeping time in mice intraperitoneally administered with sodium pentobarbital (50?mg/kg body weight). These results supported the traditional use of ZMS for the treatment of insomnia. The seeds of Z. mauritiana should be further developed as an alternative sedative and/or hypnotic product. PMID:23861716

San, Aye Moh Moh; Thongpraditchote, Suchitra; Sithisarn, Pongtip; Gritsanapan, Wandee

2013-01-01

246

In vitro screening and evaluation of antivenom phytochemicals from Azima tetracantha Lam. leaves against Bungarus caeruleus and Vipera russelli  

PubMed Central

Background Snakebites are considered a neglected tropical disease that affects thousands of people worldwide. Although antivenom is the only treatment available, it is associated with several side effects. As an alternative, plants have been extensively studied in order to obtain an alternative treatment. In folk medicine, Azima tetracantha Lam. is usually used to treat snakebites. The present study aims to provide a scientific explanation for the use of this plant against snakebite. The extracts of shade dried leaves of A. tetracantha were tested for in vitro inhibitory activity on toxic venom enzymes like phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase etc. from Bungarus caeruleus and Vipera russelli venoms. Results The ethylacetate extract rendered a significant inhibitory effect on the phosphomonoesterase, phosphodiesterase, phospholipase A2 and acetylcholinesterase enzymes. Conclusions The present study suggests that ethylacetate extract of A. tetracantha leaves possesses compounds that inhibit the activity of toxic enzymes from Bungarus caeruleus and Vipera russelli venom. Further pharmacological and in vivo studies would provide evidence that this substance may lead to a potential treatment against these venoms. PMID:24690426

2014-01-01

247

An ethyl acetate fraction of Moringa oleifera Lam. Inhibits human macrophage cytokine production induced by cigarette smoke.  

PubMed

Moringa oleifera Lam. (MO) has been reported to harbor anti-oxidation and anti-inflammatory activity and useful in the treatment of inflammatory diseases. However, despite these findings there has been little work done on the effects of MO on immune cellular function. Since macrophages, TNF and related cytokines play an important pathophysiologic role in lung damage induced by cigarette smoke, we examined the effects of MO on cigarette smoke extract (CSE)-induced cytokine production by human macrophages. An ethyl acetate fraction of MO (MOEF) was prepared from fresh leaves extract of Moringa and shown to consist of high levels of phenolic and antioxidant activities. Human monocyte derived macrophages (MDM) pre-treated with varying concentrations of MOEF showed decreased production of TNF, IL-6 and IL-8 in response to both LPS and CSE. The decrease was evident at both cytokine protein and mRNA levels. Furthermore, the extract inhibited the expression of RelA, a gene implicated in the NF-?B p65 signaling in inflammation. The findings highlight the ability of MOEF to inhibit cytokines (IL-8) which promote the infiltration of neutrophils into the lungs and others (TNF, IL-6) which mediate tissue disease and damage. PMID:24553063

Kooltheat, Nateelak; Sranujit, Rungnapa Pankla; Chumark, Pilaipark; Potup, Pachuen; Laytragoon-Lewin, Nongnit; Usuwanthim, Kanchana

2014-01-01

248

Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes.  

PubMed

Toddalia asiatica (L.) Lam. (T. asiatica) has been utilized traditionally for medicinal purposes such as the treatment of diabetes. Currently, the extract is considered to be a good source of anti-diabetic agents, but the active compounds have yet to be identified. In this study, we investigated the effects of fractionated T. asiatica extracts on the differentiation of 3T3-L1 preadipocytes and identified aculeatin as a potential active agent. When 3T3-L1 preadipocytes were treated with aculeatin isolated from T. asiatica in the presence of insulin, aculeatin increased cellular triglyceride levels and glycerol-3-phosphate dehydrogenase activity. This indicated that aculeatin could enhance the differentiation of preadipocytes into adipocytes. Further analyses using a DNA microarray and real-time quantitative reverse-transcription PCR showed an increase in the expression of peroxisome proliferator-activated receptor-? target genes (Pparg, Ap2, Cd36, Glut4 and Adipoq) by aculeatin, suggesting that aculeatin enhances the differentiation of 3T3-L1 cells by modulating the expression of genes critical for adipogenesis. Interestingly, after treatment of differentiated adipocytes with aculeatin, glucose uptake and lipolysis were enhanced. Overall, our results suggested that aculeatin is an active compound in T. asiatica for enhancing both differentiation and lipolysis of adipocytes, which are useful for the treatment of lipid abnormalities as well as diabetes. PMID:25445590

Watanabe, Akio; Kato, Tsuyoshi; Ito, Yusuke; Yoshida, Izumi; Harada, Teppei; Mishima, Takashi; Fujita, Kazuhiro; Watai, Masatoshi; Nakagawa, Kiyotaka; Miyazawa, Teruo

2014-10-14

249

An Ethyl Acetate Fraction of Moringa oleifera Lam. Inhibits Human Macrophage Cytokine Production Induced by Cigarette Smoke  

PubMed Central

Moringa oleifera Lam. (MO) has been reported to harbor anti-oxidation and anti-inflammatory activity and useful in the treatment of inflammatory diseases. However, despite these findings there has been little work done on the effects of MO on immune cellular function. Since macrophages, TNF and related cytokines play an important pathophysiologic role in lung damage induced by cigarette smoke, we examined the effects of MO on cigarette smoke extract (CSE)—induced cytokine production by human macrophages. An ethyl acetate fraction of MO (MOEF) was prepared from fresh leaves extract of Moringa and shown to consist of high levels of phenolic and antioxidant activities. Human monocyte derived macrophages (MDM) pre-treated with varying concentrations of MOEF showed decreased production of TNF, IL-6 and IL-8 in response to both LPS and CSE. The decrease was evident at both cytokine protein and mRNA levels. Furthermore, the extract inhibited the expression of RelA, a gene implicated in the NF-?B p65 signaling in inflammation. The findings highlight the ability of MOEF to inhibit cytokines (IL-8) which promote the infiltration of neutrophils into the lungs and others (TNF, IL-6) which mediate tissue disease and damage. PMID:24553063

Kooltheat, Nateelak; Pankla Sranujit, Rungnapa; Chumark, Pilaipark; Potup, Pachuen; Laytragoon-Lewin, Nongnit; Usuwanthim, Kanchana

2014-01-01

250

Enhanced biofuel production from high-concentration bioethanol wastewater by a newly isolated heterotrophic microalga, Chlorella vulgaris LAM-Q.  

PubMed

Microalgal biofuel production from wastewater has economic and environmental advantages. This article investigates the lipid production from high chemical oxygen demand (COD) bioethanol wastewater without dilution or additional nutrients, using a newly isolated heterotrophic microalga, Chlorella vulgaris LAM-Q. To enhance lipid accumulation, the combined effects of important operational parameters were studied via response surface methodology. The optimal conditions were found to be temperature of 22.8?, initial pH of 6.7, and inoculum density of 1.2 × 10(8) cells/ml. Under these conditions, the lipid productivity reached 195.96 mg/l/d, which was markedly higher than previously reported values in similar systems. According to the fatty acid composition, the obtained lipids were suitable feedstock for biodiesel production. Meanwhile, 61.40% of COD, 51.24% of total nitrogen, and 58.76% of total phosphorus were removed from the bioethanol wastewater during microalgal growth. In addition, 19.17% of the energy contained in the wastewater was transferred to the microalgal biomass in the fermentation process. These findings suggest that C. vulgaris LAMQ can efficiently produce lipids from high-concentration bioethanol wastewater, and simultaneously performs wastewater treatment. PMID:23801252

Xie, Tonghui; Liu, Jing; Du, Kaifeng; Liang, Bin; Zhang, Yongkui

2013-10-28

251

Resurgence and the Nekrasov-Shatashvili Limit: Connecting Weak and Strong Coupling in the Mathieu and Lam'e Systems  

E-print Network

The Nekrasov-Shatashvili limit for the low-energy behavior of N=2 and N=2* supersymmetric SU(2) gauge theories is encoded in the spectrum of the Mathieu and Lam'e equations, respectively. This correspondence is usually expressed via an all-orders Bohr-Sommerfeld relation, but this neglects non-perturbative effects, the nature of which is very different in the electric, magnetic and dyonic regions. In the gauge theory dyonic region the spectral expansions are divergent, and indeed are not Borel-summable, so they are more properly described by resurgent trans-series in which perturbative and non-perturbative effects are deeply entwined. In the gauge theory electric region the spectral expansions are convergent, but nevertheless there are non-perturbative effects due to poles in the expansion coefficients, and which we associate with worldline instantons. This provides a concrete analog of a phenomenon found recently by Drukker, Marino and Putrov in the large N expansion of the ABJM matrix model, in which non-pe...

Basar, Gokce

2015-01-01

252

Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of Mycobacterium tuberculosis  

PubMed Central

Objectives Moxifloxacin (MXF) has been advocated for the treatment of extensively drug-resistant (XDR) tuberculosis despite resistance to older-generation fluoroquinolones. We investigated the relationship between the minimum inhibitory concentration (MIC) of MXF and mutations in the gyrA and gyrB genes in Mycobacterium tuberculosis (MTB) isolates from KwaZulu-Natal (KZN) Province of South Africa. Materials and methods MICs of 56 MTB isolates were compared to the mutations in the quinolone resistance-determining region known to confer fluoroquinolone resistance. Isolates were genotyped by IS6110 restriction fragment length polymorphism analysis. Results The circulating F15/LAM4/KZN XDR strain circulating in KZN Province harbored the A90V mutation and displayed high-level resistance with MICs of 8 mg/L for ciprofloxacin and ofloxacin and ?1 mg/L for MXF. Conclusion The inclusion of MXF in XDR-TB treatment regimens requires careful consideration in our setting, where clinical outcome data in MXF-containing regimens are unavailable. PMID:25187730

Dookie, Navisha; Sturm, A Willem; Moodley, Prashini

2014-01-01

253

(±)-catechin, a root exudate of the invasive centaurea stoebe lam. (Spotted knapweed) exhibits bacteriostatic activity against multiple soil bacterial populations.  

PubMed

Understanding the effects of allelopathic plant chemicals on soil microorganisms is critical to understanding their ecological roles and importance in exotic plant invasion. Centaurea stoebe Lam. (spotted knapweed), an aggressive invasive weed in North America, secretes a racemic mixture of (±)-catechin as a root exudate. This enantiomeric, polyphenolic compound has been reported to have allelopathic effects on surrounding flora and microflora. To better understand how catechin affects microbial communities in the root zone of spotted knapweed, we assessed its impact on the total culturable bacterial component and numerous individual bacterial populations from Romanian (native range) and Montana (invaded range) soils. Catechin suppressed total culturable count numbers from the bacterial community and inhibited growth of some, but not all, soil bacterial populations tested. The native soil bacterial community was significantly more resistant to inhibitory effects of catechin than either the invaded or non-invaded soils. We further show that the inhibitory effect of catechin on nine different soil bacterial strains from seven genera was reversible, demonstrating that it acts via a bacteriostatic rather than bactericidal mechanism. These findings suggest that catechin might affect bacterial community composition and activity in the root zone. PMID:21882071

Pollock, Jarrod L; Kogan, Lewis A; Thorpe, Andrea S; Holben, William E

2011-09-01

254

Immunogenicity of viral B-cell epitopes inserted into two surface loops of the Escherichia coli K12 LamB protein and expressed in an attenuated aroA strain of Salmonella typhimurium.  

PubMed

We previously developed a general procedure which allows the genetic coupling of a chosen foreign linear epitope in different 'permissive' sites of a carrier protein. By using the outer membrane protein LamB of Escherichia coli K12 as a carrier, we were able to express a number of different foreign epitopes at the bacterial surface. In the present work, taking advantage of the recent determination of the crystal structure of LamB, we inserted two model B-cell epitopes i.e.--the C3 epitope from poliovirus (residues 93 to 103 of VP1) and the preS2 epitope from hepatitis B virus, (residues 132 to 145)--at the tip of the most distal and largest surface exposed region of LamB (after residues 386, into loop L9). We also used two previously constructed LamB hybrids, corresponding to the insertion of the C3B or preSB epitope into permissive site 153 (lying in the middle of the fourth surface loop of LamB), to construct two LamB proteins corresponding to the simultaneous insertion of the two different epitopes (with one epitope per site). The LamB hybrids were placed under the control of the anaerobically inducible pnirB promoter and expressed in a LamB-negative derivative of the aroA attenuated strain of S. typhimurium, SL3261. In vitro, the recombinant proteins were expressed at a high level (up to 10% of whole cell proteins) and in vivo the recombinant plasmids were stably maintained. For both epitopes, genetic coupling at site 386 appeared to be more favorable for the induction of anti-epitope antibodies than coupling at site 153. Moreover, the LamB hybrid corresponding to the simultaneous insertion of the preSB epitope at site 153 and of the C3B epitope at site 386 allowed the induction of both anti-poliovirus and anti-hepatitis B antibodies. PMID:10078601

Wang, J; Michel, V; Leclerc, C; Hofnung, M; Charbit, A

1999-01-01

255

Improvement of the coagulation/flocculation process using a combination of Moringa oleifera lam with anionic polymer in water treatment.  

PubMed

The objective of this study is to investigate the impacts of anionic polymer as a flocculant aid on the coagulation/flocculation performance with a saline solution of Moringa oleifera as a coagulant to provide larger flocs and decrease the time sedimentation. For the tests, raw water was used from Pirapó River Basin (Maringá, Paraná, Brazil). Optimization of coagulation/flocculation tests was initially performed in a jar-test with a dosage of M. oleifera Lam (crude extract--MO, oil-extracted with ethanol--MO (et) and hexane--MO (hex) 1% m/v) as the coagulant that ranged from 10 to 60 mg L(-1) and of the anionic polymer 0.1% as a flocculant aid with a dosage that ranged from 0 to 0.4 mg L(-1). The parameters analysed were colour, turbidity and compounds with absorption in UV254nm. In view of the statistical analysis results, MO (hex) with a dosage of 30 mg L(-1) was chosen as a coagulant for the next tests of coagulation/flocculation. When anionic polymer was used alone (0.0 mg L(-1) of MO (hex)), parameters were not removed and there was no generation of heavy flocs as compared with the combination of MO (hex) with the anionic polymer. Statistical analysis showed that MO (hex) obtained the highest removals of the parameters analysed in lower dosages and no significant increase in parameters removal was observed when the polymer dosage was increased. The efficacy of the coagulant +/- anionic polymer was optimal when 30mg L(-1) of MO (hex) was used as a coagulant and 0.1 mg L(-1) of the anionic polymer was used as a flocculant aid, decreasing the time sedimentation from 1 h to 15 min. PMID:25145175

Bongiovani, Milene Carvalho; Camacho, Franciele Pereira; Nishi, Letícia; Coldebella, Priscila Ferri; Valverde, Karina Cardoso; Vieira, Angélica Marquetotti Salcedo; Bergamasco, Rosângela

2014-01-01

256

Identification of a Proteinaceous Component in the Leaf of Moringa Oleifera lam. with Effects on High Serum Creatinine.  

PubMed

Moringa oleifera Lam. has been an important plant in the history of mankind, both for its nutritional and medicinal uses. Apart from bactericidal effects, the parts of this plant have been effectively used in the treatment of circulatory, respiratory, endocrine, digestive as well as neural disorders. Till date, though, there has been no reported activity of the involvement of any proteinaceous extract from M. oleifera on high levels of serum creatinine. To address this issue, blood samples with high levels of serum creatinine (2 mg/dl and above) were treated with leaf extract from M. oleifera. The crude extract was partially purified initially and eventually purified to completion as well. All these proteinaceous fractions were used to treat samples with high levels of serum creatinine as mentioned above. While the treatment of serum sample having high creatinine with crude extract and partially purified protein fractions showed a decrease of approximately 20% in the levels of serum creatinine over a period of 24 h, the samples treated with purified protein fraction reduced the serum creatinine level by 50%. In light of the fact that increased level of serum creatinine levels have adverse downstream effects on the heart, lungs and other organs, this communication assumes significance because it suggests a way of reducing the level of serum creatinine as an emergency measure. Further, the identification and characterisation of this proteinaceous component and possible in vivo experiments would provide a major tool for the treatment of downstream complications associated with increased serum creatinine via a new sources, albeit a natural one. PMID:24799742

Sahoo, S; Raghavendra, K M; Biswas, S

2014-01-01

257

Anti-apoptosis effect of polysaccharide isolated from the seeds of Cuscuta chinensis Lam on cardiomyocytes in aging rats.  

PubMed

To investigate the mechanism of apoptosis in myocardial cells of aging rats induced by D-galactose and to study the effect of the Polysaccharide isolated from the seeds of Cuscuta chinensis Lam (PCCL) on apoptosis of cardiomyocytes and its corresponding machinasim in aging rat model. Fifty male SD rats were randomly divided into 5 groups. Normal control group (NC). D-galactose (100 mg · kg(-1)d(-1) for 56 day) indued aging group (MC), D-galactose plus 100 mg kg(-1) d(-1) PCCL group (ML), D-galactose plus 200 mg kg(-1) d(-1) PCCL group (MM), and D-galactose plus 400 mg kg(-1) d(-1) PCCL group (MH). Same volume of solution (water, or PCCL aqueous solution) was given by gavage for 56 days. Then the hearts were collected and apoptosis parameters were evaluated. Caspase-3 and Cyt c were determined by fluorescence spectrometer, the apoptosis rate was assessed by AnnexinV-FITC method by Flow-Cytometry, [Ca(2+)]i and [Ca(2+)]i overloaded by KCL were observed by laser scanning confocal microscopy (LSCM); Bcl-2 and Bax were examined by immunohistochemistry. The content of Cyt C, [Ca(2+)]i of cardiomyocytes, the activity of Caspase-3, Bax expression level in D-galactose induced aging group were higher than NC (p < 0.05). The ratio of Bcl-2/Bax was decreased in D-galactose induced aging group compared to NC. On the other hand, the content of Cyt C, [Ca(2+)]i of cardiomyocytes, the activity of Caspase-3 and apoptosis rate, as well as Bax expression level in all three PCCL groups were decreased compared to galactose induced group (p < 0.05). Bcl-2/Bax ratio was increased in all PCCL groups compared to galactose induced aging group. PCCL could decrease the apoptosis of cardiomyocytes by the mitochondria apoptosis pathway. PMID:24972571

Sun, Shou-Li; Guo, Li; Ren, Ya-Chao; Wang, Bing; Li, Rong-Hui; Qi, Yu-Shan; Yu, Hui; Chang, Nai-Dan; Li, Ming-Hui; Peng, Hai-Sheng

2014-09-01

258

A comparative study of proteomic differences between pencil and storage roots of sweetpotato (Ipomoea batatas (L.) Lam.).  

PubMed

Fibrous roots of sweetpotato (Ipomoea batatas (L.) Lam.) usually develop into both pencil and storage roots. To understand protein function in root development, a proteomic analysis was conducted on the pencil and storage roots of the light orange-fleshed sweetpotato cultivar, Yulmi. Two-dimensional gel electrophoresis showed that expression of 30 protein spots differed between pencil and storage roots: 15 proteins were up-regulated or expressed in pencil roots and 15 in storage roots. Differentially expressed proteins spots were investigated using matrix-assisted laser desorption/ionization time of flight mass spectrometry, and 10 proteins from pencil roots were identified as binding protein isoform A, catechol oxidase, peroxidases, ascorbate peroxidase, endochitinase, flavanone 3-hydroxylase and unknown proteins. Of the proteins up-regulated in, or restricted to, storage roots, 13 proteins were identified as protein disulfide isomerase, anionic peroxidase, putative ripening protein, sporamin B, sporamin A and sporamin A precursor. An analysis of enzyme activity revealed that catechol oxidase and peroxidase as the first and last enzymes of the lignin biosynthesis pathway, and ascorbate peroxidase had higher activities in pencil than in storage roots. The total concentration of phenolic compounds was also far higher in pencil than in storage roots, and lignin accumulated only in pencil roots. These results provide important insight into sweetpotato proteomics, and imply that lignin biosynthesis and stress-related proteins are up-regulated or uniquely expressed in pencil roots. The results indicate that the reduction of carbon flow toward phenylpropanoid biosynthesis and its delivery to carbohydrate metabolism is a major event in storage root formation. PMID:25562766

Lee, Jeung Joo; Kim, Yun-Hee; Kwak, Youn-Sig; An, Jae Young; Kim, Pil Joo; Lee, Byung Hyun; Kumar, Vikranth; Park, Kee Woong; Chang, Eun Sil; Jeong, Jae Cheol; Lee, Haeng-Soon; Kwak, Sang-Soo

2015-02-01

259

Altitudinal limits of Berberis L. in Tajikistan  

Microsoft Academic Search

The paper reports on distribution of 8 wild barberry species in phytogeographic regions of Tajikistan, specifies altitudinal\\u000a limits of the barberry species and vegetation types the species are components of.

S. Kh. Davlatov; E. V. Baikova

2011-01-01

260

Identification of a novel major locus for gray leaf spot resistance in Italian ryegrass ( Lolium multiflorum Lam.).  

PubMed

BackgroundGray leaf spot (GLS), caused by Magnaporthe oryzae (anamorph Pyricularia oryzae), in ryegrasses is a very serious problem. Heavily infected small seedlings die within a matter of days, and stands of the grasses are seriously damaged by the disease. Thus, the development of GLS-resistant cultivars has become a concern in ryegrass breeding.ResultsPhenotypic segregations in a single cross-derived F1 population of Italian ryegrass (Lolium multiflorum Lam.) indicated that the GLS resistance in the population was possibly controlled by one or two dominant genes with 66.5¿77.9% of broad-sense heritability. In bulked segregant analyses, two simple sequence repeat (SSR) markers, which have so far been reported to locate on linkage group (LG) 3 of Italian ryegrass, showed specific signals in the resistant parent and resistant bulk, indicating that the resistance gene locus was possibly in the LG 3. We thus constructed a genetic linkage map of the LG 3 covering 133.6 centimorgan with other SSR markers of the LG 3 of Italian ryegrass and grass anchor probes that have previously been assigned to LG 3 of ryegrasses, and with rice expressed sequence tag (EST)-derived markers selected from a rice EST map of chromosome (Chr) 1 since LG 3 of ryegrasses are syntenic to rice Chr 1. Quantitative trait locus (QTL) analysis with the genetic linkage map and phenotypic data of the F1 population detected a major locus for GLS resistance. Proportions of phenotypic variance explained by the QTL at the highest logarithm of odds scores were 61.0¿69.5%.ConclusionsA resistance locus was confirmed as novel for GLS resistance, because its genetic position was different from other known loci for GLS resistance. Broad-sense heritability and the proportion of phenotypic variance explained by the QTL were similar, suggesting that most of the genetic factors for the resistance phenotype against GLS in the F1 population can be explained by a function of the single resistance locus. We designated the putative gene for the novel resistance locus as LmPi2. LmPi2 will be useful for future development of GLS-resistant cultivars in combination with other resistance genes. PMID:25407403

Takahashi, Wataru; Miura, Yuichi; Sasaki, Tohru; Takamizo, Tadashi

2014-11-18

261

Correlations among attributes of senescence and antioxidative status of leaf discs during epiphyllous bud differentiation in Kalanchoe pinnata Lam. (Pers.).  

PubMed

Leaf detachment is a common signal that triggers both the differentiation of dormant epiphyllous buds as well as the onset of foliar senescence in Kalanchoe pinnata Lam. (Pers.). The present study looked for any probable correlations among selected attributes of foliar senescence, e.g. soluble proteins, chlorophylls a and b (Chl(a+b)), and membrane stability index (MSI), and the antioxidative status, e.g. phenolics, ferric reducing ability in plasma equivalence (FRAP(eq)), and membrane protection index (MPI), during epiphyllous bud differentiation. The experimental system comprised 0.75-cm leaf discs, with or without a dormant epiphyllous bud, cultured in vitro and exposed for ten days to continuous light or dark. A steady depletion of soluble proteins and Chl(a+b), and lowering of MSI in the leaf discs were observed, the decline being relatively faster and of higher magnitude in discs exposed to dark rather than to light. The pigment loss in discs with differentiating epiphyllous buds was greater and faster than in those lacking buds, a somewhat reverse situation was observed in case of soluble proteins. Simultaneously, a time-dependent decrease in the level of phenolics was also observed. Their content was found to be lower in discs exposed to dark as compared to light, pointing to a relationship with a higher rate of senescence-related degradative processes in the dark. The change in the content of Chl(a+b) was found to be significantly correlated with the variation in the level of phenolics. The average FRAP(eq) after ten days was one half that of the initial level, which could be correlated with the decreasing levels of phenolics (intra-correlation) and maximally correlated with variations in Chl(a+b) and protein contents (inter-correlation). Aqueous alcohol foliar extracts significantly (p < 0.05) protected membranes against peroxidative stress, although the pattern was not found to be in line with that of the phenolics content or FRAP(eq). The diminishing Chl(a+b) content was found to be maximally correlated with alterations in the membrane protection. PMID:23016282

Jaiswal, Sarita; Chawla, Raman; Sawhney, Sudhir

2012-01-01

262

Effect of Moringa oleifera Lam. seed extract on toluene diisocyanate-induced immune-mediated inflammatory responses in rats.  

PubMed

Moringa oleifera Lam. is a small tree cultivated throughout India. We have investigated the effect of ethanolic extract of seeds of Moringa oleifera (MOEE, an herbal remedy) on the potential prevention of immune-mediated inflammatory responses in toluene diisocyanate (TDI as antigen)-induced asthma in Wistar rats. Rats were divided into five different groups (n = 8/group): Group-I = unsensitized control; Group-II = TDI control/vehicle; Group-III = dexamethasone (DXM) 2.5 mg/kg; and, Groups IV and V = 100 mg/kg and 200 mg/kg body weight [BW] of MOEE, respectively. All rats (except unsensitized controls) were sensitized by intranasal application of 10% TDI to induce airway hypersensitivity. Animals in Groups II-V were given their respective drug treatment per os from 1 wk prior to initiation of sensitization until the day of final provocation with 5% TDI. After this last challenge, all rats were examined for hyperreactivity symptoms and then sacrificed to determine their total and differential leucocytes in blood and bronchoalveolar (BAL) fluid and levels of TNF proportional, variant, IL-4, and IL-6 in their BAL and serum. Homogenates of one lung lobe from each animal were utilized to assess oxidative stress; a separate lobe underwent histologic examination to assess airway inflammatory status. The results suggest that asthmatic symptoms were found in TDI control rats only, while both MOEE- and DXM-treated rats did not manifest any airway abnormality. In MOEE- and DXM-treated rats, neutrophil and eosinophil levels in the blood were decreased significantly; levels of total cells and each different cell in their BAL fluid were markedly decreased as compared to those in TDI controls. TNF alpha, IL-4, and IL-6 were predominant in serum as well as in BAL fluids in TDI controls, but these levels were reduced significantly by MOEE treatment. The antioxidant activity in relation to antiinflammatory activity of the extract and histopathological observations also reflected a protective effect. Based on the above findings and observations, it can be concluded that Moringa oleifera may possess some beneficial properties that act against chemically stimulated immune-mediated inflammatory responses that are characteristic of asthma in the rat. PMID:18958717

Mahajan, Shailaja G; Mali, Ravindra G; Mehta, Anita A

2007-04-01

263

Evaluation of Visual Inspection with Acetic Acid (VIA), Lugol's iodine (VILI), Cervical Cytology and HPV Testing as Cervical Screening Tools in Latin America. This Report Refers to Partial Results from the LAMS (Latin AMerican Screening) Study  

Microsoft Academic Search

Objectives: To assess the performance indicators of visual inspection with acetic acid (VIA) and visual inspection with Lugol's iodine (VILI) in four Latin American centres participating in the ongoing Latin AMerican Screening (LAMS) study, in settings with moderate incidence of cervical disease and with poorly to moderately well-organized cervical cancer screening. Setting: Three Brazilian centres (Sao Paulo, Campinas and Porto

LO Sarian; SF Derchain; P Naud; C Roteli-Martins; A Longatto-Filho; S Tatti; M Branca; M Erzen; L Serpa-Hammes; J Matos; R C Gontijo; JF Braganca; TP Lima; M Y S Maeda; A Lorincz; GB Dores; S Costa; S Syrjanen; K Syrjanen

2006-01-01

264

Determination of hyperin in seed of Cuscuta chinensis Lam. by enhanced chemiluminescence of CdTe quantum dots on calcein/K3Fe(CN)6 system.  

PubMed

In this paper, 3-mercaptocarboxylic acid (MPA) modified CdTe quantum dots (QDs) were used as sensitizers, to enhance the chemiluminescence (CL) of the calcein/K(3)Fe(CN)(6) system. A new CL system of CdTe/calcein/K(3)Fe(CN)(6) was developed. The effects of reactant concentrations and the particle sizes of CdTe QDs on the CL emission were investigated in detail. The possible enhancement mechanism of the CL was also further investigated based on the photoluminescence (PL) and CL spectra. Polyphenols such as chlorogenic acid, quercetin, hyperin, catechin and kaempferol, were observed to inhibit the CL signal of the CdTe/calcein/K(3)Fe(CN)(6) system and determined by the proposed method. The proposed method was applied to the determination of hyperin in seed of Cuscuta chinensis Lam. and the results obtained were satisfactory. PMID:23442699

Kang, Jing; Li, Xuwen; Geng, Jiayang; Han, Lu; Tang, Jieli; Jin, Yongri; Zhang, Yihua

2012-10-15

265

Symmetry-breaking 60°-spin order in the A-site-ordered perovskite LaM n3V4O12  

NASA Astrophysics Data System (ADS)

The magnetism of the A-site-ordered perovskite LaM n3V4O12 is studied comprehensively by means of neutron powder diffraction experiments and theoretical calculations. Magnetic neutron diffraction results show that a rhombohedral 60° spin structure emerges on the cubic lattice below a 44-K Néel transition. Ab initio electronic structure calculations confirm that high-spin Mn2 + moments are localized while V 3 d -band states are itinerant, and that the noncollinear 60° spin structure is more stable than collinear ferromagnetic or G-type antiferromagnetic alternatives. Effective Heisenberg model calculations reveal that the appearance of such a nontrivial spin structure can be attributed to significant next-nearest-neighbor and third-nearest-neighbor magnetic interactions.

Saito, Takashi; Toyoda, Masayuki; Ritter, Clemens; Zhang, Shoubao; Oguchi, Tamio; Attfield, J. Paul; Shimakawa, Yuichi

2014-12-01

266

Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts  

PubMed Central

Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro???-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36 ± 0.01 and 1.83 ± 0.03?mg/mL and IC90 values of 2.12 ± 0.04 and 2.62 ± 0.09?mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47 ± 0.006 and 0.71 ± 0.006?mg/mL, respectively. PMID:24558335

Mojarrab, Mahdi; Shiravand, Ali; Delazar, Abbas

2014-01-01

267

Evaluation of in vitro antimalarial activity of different extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and fractions of the most potent extracts.  

PubMed

Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro?? ? -hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36±0.01 and 1.83±0.03?mg/mL and IC90 values of 2.12±0.04 and 2.62±0.09?mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47±0.006 and 0.71±0.006?mg/mL, respectively. PMID:24558335

Mojarrab, Mahdi; Shiravand, Ali; Delazar, Abbas; Heshmati Afshar, Fariba

2014-01-01

268

Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves.  

PubMed

Water, aqueous methanol, and aqueous ethanol extracts of freeze-dried leaves of Moringa oleifera Lam. from different agroclimatic regions were examined for radical scavenging capacities and antioxidant activities. All leaf extracts were capable of scavenging peroxyl and superoxyl radicals. Similar scavenging activities for different solvent extracts of each collection were found for the stable 1,1-diphenyl 2-picrylhydrazyl (DPPH(*)) radical. Among the three different moringa samples, both methanol and ethanol extracts of Indian origins showed the highest antioxidant activities, 65.1 and 66.8%, respectively, in the beta-carotene-linoleic acid system. Nonetheless, increasing concentration of all the extracts had significantly (P < 0.05) increased reducing power, which may in part be responsible for their antioxidant activity. The major bioactive compounds of phenolics were found to be flavonoid groups such as quercetin and kaempferol. On the basis of the results obtained, moringa leaves are found to be a potential source of natural antioxidants due to their marked antioxidant activity. This is the first report on the antioxidant properties of the extracts from freeze-dried moringa leaves. Overall, both methanol (80%) and ethanol (70%) were found to be the best solvents for the extraction of antioxidant compounds from moringa leaves. PMID:12670148

Siddhuraju, Perumal; Becker, Klaus

2003-04-01

269

Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam.  

PubMed

Medicinal plants have been shown to have both chemopreventive and/or therapeutic effects on cancer and other diseases related to oxidative damage. Moringa oleifera Lam., known in the Hausa and Igala languages of Nigeria as "Zogale" and "Gergedi," respectively, and drumstick in English, is a plant that is used both as food and in folkloric medicine in Nigeria and elsewhere. Different parts of the plant were analyzed for polyphenol content as well as in vitro antioxidant potential. The methanol extract of the leaves of M. oleifera contained chlorogenic acid, rutin, quercetin glucoside, and kaempferol rhamnoglucoside, whereas in the root and stem barks, several procyanidin peaks were detected. With the xanthine oxidase model system, all the extracts exhibited strong in vitro antioxidant activity, with 50% inhibitory concentration (IC(50)) values of 16, 30, and 38 microL for the roots, leaves, and stem bark, respectively. Similarly, potent radical scavenging capacity was observed when extracts were evaluated with the 2-deoxyguanosine assay model system, with IC(50) values of 40, 58, and 72 microL for methanol extracts of the leaves, stem, and root barks, respectively. The high antioxidant/radical scavenging effects observed for different parts of M. oleifera appear to provide justification for their widespread therapeutic use in traditional medicine in different continents. The possibility that this high antioxidant/radical scavenging capacity may impact on the cancer chemopreventive potential of the plant must be considered. PMID:20521992

Atawodi, Sunday E; Atawodi, Joy C; Idakwo, Gabriel A; Pfundstein, Beate; Haubner, Roswitha; Wurtele, Gerd; Bartsch, Helmut; Owen, Robert W

2010-06-01

270

Eugenia jambolana Lam. Berry Extract Inhibits Growth and Induces Apoptosis of Human Breast Cancer but not Non-Tumorigenic Breast Cells  

PubMed Central

The ripe purple berries of the native Indian plant, Eugenia jambolana Lam., known as Jamun, are popularly consumed and available in the United States in Florida and Hawaii. Despite the growing body of data on the chemopreventive potential of edible berry extracts, there is paucity of such data for Jamun fruit. Therefore our laboratory initiated the current study with the following objectives:1) to prepare a standardized Jamun fruit extract (JFE) for biological studies and, 2) to investigate the anti-proliferative and pro-apoptotic effects of JFE in estrogen dependent/aromatase positive (MCF-7aro), and estrogen independent (MDA-MB-231) breast cancer cells, and in a normal/non-tumorigenic (MCF-10A) breast cell line. JFE was standardized to anthocyanin content using the pH differential method, and individual anthocyanins were identified by high performance liquid chromatography with ultraviolet (HPLC-UV) and tandem mass spectrometry (LC-MS/MS) methods. JFE contained 3.5% anthocyanins (as cyanidin-3-glucoside equivalents) which occur as diglucosides of five anthocyanidins/aglycons: delphinidin, cyanidin, petunidin, peonidin and malvidin. In the proliferation assay, JFE was most effective against MCF-7aro (IC50=27 µg/mL), followed by MDA-MB-231 (IC50=40 µg/mL) breast cancer cells. Importantly, JFE exhibited only mild antiproliferative effects against the normal MCF-10A (IC50>100 µg/mL) breast cells. Similarly, JFE (at 200 µg/mL) exhibited pro-apoptotic effects against the MCF-7aro (p?0.05) and the MDA-MB-231 (p?0.01) breast cancer cells, but not towards the normal MCF-10A breast cells. These studies suggest that JFE may have potential beneficial effects against breast cancer. PMID:19166352

Li, Liya; Adams, Lynn S.; Chen, Shiuan; Killian, Caroline; Ahmed, Aftab; Seeram, Navindra P.

2009-01-01

271

Effects of Elevated CO2 and N Addition on Growth and N2 Fixation of a Legume Subshrub (Caragana microphylla Lam.) in Temperate Grassland in China  

PubMed Central

It is well demonstrated that the responses of plants to elevated atmospheric CO2 concentration are species-specific and dependent on environmental conditions. We investigated the responses of a subshrub legume species, Caragana microphylla Lam., to elevated CO2 and nitrogen (N) addition using open-top chambers in a semiarid temperate grassland in northern China for three years. Measured variables include leaf photosynthetic rate, shoot biomass, root biomass, symbiotic nitrogenase activity, and leaf N content. Symbiotic nitrogenase activity was determined by the C2H2 reduction method. Elevated CO2 enhanced photosynthesis and shoot biomass by 83% and 25%, respectively, and the enhancement of shoot biomass was significant only at a high N concentration. In addition, the photosynthetic capacity of C. microphylla did not show down-regulation under elevated CO2. Elevated CO2 had no significant effect on root biomass, symbiotic nitrogenase activity and leaf N content. Under elevated CO2, N addition stimulated photosynthesis and shoot biomass. By contrast, N addition strongly inhibited symbiotic nitrogenase activity and slightly increased leaf N content of C. microphylla under both CO2 levels, and had no significant effect on root biomass. The effect of elevated CO2 and N addition on C. microphylla did not show interannual variation, except for the effect of N addition on leaf N content. These results indicate that shoot growth of C. microphylla is more sensitive to elevated CO2 than is root growth. The stimulation of shoot growth of C. microphylla under elevated CO2 or N addition is not associated with changes in N2-fixation. Additionally, elevated CO2 and N addition interacted to affect shoot growth of C. microphylla with a stimulatory effect occurring only under combination of these two factors. PMID:22046376

Zhang, Lin; Wu, Dongxiu; Shi, Huiqiu; Zhang, Canjuan; Zhan, Xiaoyun; Zhou, Shuangxi

2011-01-01

272

Phosphorus status and microbial community of paddy soil with the growth of annual ryegrass (Lolium multiflorum Lam.) under different phosphorus fertilizer treatments*  

PubMed Central

Annual ryegrass (Lolium multiflorum Lam.) was grown in paddy soil in pots under different phosphorus (P) fertilizer treatments to investigate changes of P fractions and microbial community of the soil. The treatments included Kunyang phosphate rock (KPR) applications at 50 mg P/kg (KPR50) and 250 mg P/kg (KPR250), mono-calcium phosphate (MCP) application at 50 mg P/kg (MCP50), and the control without P application. The results showed that KPR50, KPR250, and MCP50 applications significantly increased the dry weight of the ryegrass by 13%, 38%, and 55%, and increased P uptake by 19%, 135%, and 324%, respectively. Compared with MCP50, the relative effectiveness of KPR50 and KPR250 treatments in ryegrass production was about 23% and 68%, respectively. After one season of ryegrass growth, the KPR50, KPR250, and MCP50 applications increased soil-available P by 13.4%, 26.8%, and 55.2%, respectively. More than 80% of the applied KPR-P remained as HCl-P fraction in the soil. Phospholipid fatty acid (PLFA) analysis showed that the total and bacterial PLFAs were significantly higher in the soils with KPR250 and MCP50 treatments compared with KPR50 and control. The latter had no significant difference in the total or bacterial PLFAs. The KPR50, KPR250, and MCP50 treatments increased fungal PLFA by 69%, 103%, and 69%, respectively. Both the principal component analysis and the cluster analysis of the PLFA data suggest that P treatments altered the microbial community composition of the soils, and that P availability might be an important contributor to the changes in the microbial community structure during the ryegrass growth in the paddy soils. PMID:19817001

Guo, Hai-chao; Wang, Guang-huo

2009-01-01

273

Eugenia jambolana Lam. berry extract inhibits growth and induces apoptosis of human breast cancer but not non-tumorigenic breast cells.  

PubMed

The ripe purple berries of the native Indian plant Eugenia jambolana Lam., known as Jamun, are popularly consumed and available in the United States in Florida and Hawaii. Despite the growing body of data on the chemopreventive potential of edible berry extracts, there is paucity of such data for Jamun fruit. Therefore our laboratory initiated the current study with the following objectives: (1) to prepare a standardized Jamun fruit extract (JFE) for biological studies and (2) to investigate the antiproliferative and pro-apoptotic effects of JFE in estrogen dependent/aromatase positive (MCF-7aro), and estrogen independent (MDA-MB-231) breast cancer cells, and in a normal/nontumorigenic (MCF-10A) breast cell line. JFE was standardized to anthocyanin content using the pH differential method, and individual anthocyanins were identified by high performance liquid chromatography with ultraviolet (HPLC-UV) and tandem mass spectrometry (LC-MS/MS) methods. JFE contained 3.5% anthocyanins (as cyanidin-3-glucoside equivalents) which occur as diglucosides of five anthocyanidins/aglycons: delphinidin, cyanidin, petunidin, peonidin and malvidin. In the proliferation assay, JFE was most effective against MCF-7aro (IC(50) = 27 microg/mL), followed by MDA-MB-231 (IC(50) = 40 microg/mL) breast cancer cells. Importantly, JFE exhibited only mild antiproliferative effects against the normal MCF-10A (IC(50) > 100 microg/mL) breast cells. Similarly, JFE (at 200 microg/mL) exhibited pro-apoptotic effects against the MCF-7aro (p

Li, Liya; Adams, Lynn S; Chen, Shiuan; Killian, Caroline; Ahmed, Aftab; Seeram, Navindra P

2009-02-11

274

In vitro analysis on bactericidal screening and antioxidant potentiality of leaf and root extracts of Thottea siliquosa (Lam.) Ding Hou. An ethnobotanical plant  

PubMed Central

Objective Natural products of plant origin are potential source of novel antimicrobial and antioxidative agents. Thottea siliquosa (Lam.) Ding Hou. (T. siliquosa). A medicinal herb used by local tribals for treating various ailments. The present study aims at the phytochemical screening, GC-MS analysis, in vitro antibacterial activity and antioxidant potentiality of root and leaf extracts of T. siliquosa. Methods Hot continuous Soxhlet extraction, GC-MS analysis, antibacterial analysis by disc diffusion, microdilution assay and antioxidant potentialities by hydroxyl radical and nitric oxide radical scavenging. The data was statistically analyzed. Results Phytochemical screening of the ethyl acetate and methanolic extract of leaf and root revealed the presence of phenols, alkaloids, tannins and saponin. The extract revealed a pool of phytochemicals by comparison with authentic standards from spectral library. Both the extracts has shown their broad spectrum of inhibition against the selected bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia compared with standard antibiotic drug streptomycin. The extracts showed antioxidant activity by scavenging of free radicals such as hydroxyl and nitric oxide. The IC50 values of the ethyl acetate extracts leaf and root and standard in this assay were 167.5±0.67, 99.4±1.2, 192±2.5 µg/mL respectively. Similarly those methanolic extracts of leaf and root were 269.5±0.89 and 289.1±2.66 µg/mL respectively. Similarly, ethyl acetate and methanolic extracts also caused a moderate dose-dependent inhibition of nitric oxide with an IC50 range 65.5±1.55 to 148 ±3.09 µg/mL. The inhibitory activities were found to be dose dependent. Conclusion The present study provides evidence that ethyl acetate and methanol extract of leaf and root of T. siliquosa are potential source of natural antioxidants and bactericidal nature. It is essential that research should continue to isolate and purify the bio active components of this natural plant and use in drug discovery and development.

Nusaiba, Saiba Abdul Wahab; Murugan, Kumaraswamy

2013-01-01

275

Ethnobotanical studies on Berberis aristata DC. root extracts  

Microsoft Academic Search

-3 mg\\/ml and for Aspergillus species, it was 3 ? 10 -3 mg\\/ml. All three extracts also had antifungal activity against the fungal species tested, except Candida krusei. The extracts of B. aristata also demonstrated anti-inflammatory, analgesic, and antipyretic activities. Chemical analysis revealed the presence of alkaloids, amino acids, tannins, terpenes, resins, phenols and reducing sugars as major compounds. FTIR-spectral

M. Shahid; T. Rahim; A. Shahzad; A. Latif; T. Fatma; M. Rashid; Adil Raza; S. Mustafa

2009-01-01

276

Phylogenetic diversity of rhizobia associated with horsegram [Macrotyloma uniflorum (Lam.) Verdc.] grown in South India based on glnII, recA and 16S-23S intergenic sequence analyses.  

PubMed

Horsegram [Macrotyloma uniflorum (Lam.) Verdc.) is an important grain legume and fodder crop in India. Information on root nodule endosymbionts of this legume in India is limited. In the present study, 69 isolates from naturally occurring root nodules of horsegram collected from two agro-eco-climatic regions of South India was analyzed by generation rate, acid/alkali reaction on YMA medium, restriction fragment length polymorphism analysis of 16S-23S rDNA intergenic spacer region (IGS), and sequence analyses of IGS and housekeeping genes glnII and recA. Based on the rDNA IGS RFLP by means of three restriction enzymes rhizobia were grouped in five clusters (I-V). By sequence analysis of 16S-23S rDNA IGS identified genotypes of horsegram rhizobia were distributed into five divergent lineages of Bradyrhizobium genus which comprised (I) the IGS type IV rhizobia and valid species B. yuanmingense, (II) the strains of IGS type I and Bradyrhizobium sp. ORS 3257 isolated from Vigna sp., (III) the strains of the IGS type II and Bradyrhizobium sp. CIRADAc12 from Acacia sp., (IV) the IGS type V strains and Bradyrhizobium sp. genospecies IV, and (V) comprising genetically distinct IGS type III strains which probably represent an uncharacterized new genomic species. Nearly, 87% of indigenous horsegram isolates (IGS types I, II, III, and V) could not be related to any other species within the genus Bradyrhizobium. Phylogeny based on housekeeping glnII and recA genes confirmed those results found by the analysis of the IGS sequence. All the isolated rhizobia nodulated Macrotyloma sp. and Vigna spp., and only some of them formed nodules on Arachis hypogeae. The isolates within each IGS type varied in their ability to fix nitrogen. Selection for high symbiotic effective strains could reward horsegram production in poor soils of South India where this legume is largely cultivated. PMID:21188591

Appunu, Chinnaswamy; Ganesan, Govindan; Kalita, Micha?; Kaushik, Raghavan; Saranya, Balamurugan; Prabavathy, Vaiyapuri Ramalingam; Sudha, Nair

2011-04-01

277

Composition and antioxidant activity of the anthocyanins of the fruit of Berberis heteropoda Schrenk.  

PubMed

In present study, the anthocyanin composition and content of the fruit of B. heteropoda Schrenk were determined for the first time. The total anthocyanins were extracted from the fruit of B. heteropoda Schrenk using 0.5% HCl in 80% methanol and were then purified using an AB-8 macroporous resin column. The purified anthocyanin extract (PAE) was evaluated by high-performance liquid chromatography with a diode array detector (HPLC-DAD) and HPLC-high resolution-electrospray ionization-mass spectrometry (HPLC-HR-ESI-MS) under the same experimental conditions. The results revealed the presence of seven different anthocyanins. The major anthocyanins purified by preparative HPLC were confirmed to be delphinidin-3-O-glucopyranoside (30.3%), cyanidin-3-O-glucopyranoside (33.5%), petunidin-3-?-glucopyranoside (10.5%), peonidin-3-O-glucopyranoside (8.5%) and malvidin-3-O-glucopyranoside (13.8%) using HPLC-HR-ESI-MS and NMR spectroscopy. The total anthocyanin content was 2036.6 ± 2.2 mg/100 g of the fresh weight of B. heteropoda Schrenk fruit. In terms of its total reducing capacity assay, DPPH radical-scavenging activity assay, ferric-reducing antioxidant power (FRAP) assay and ABTS radical cation-scavenging activity assay, the PAE also showed potent antioxidant activity. The results are valuable for illuminating anthocyanins composition of B. heteropoda Schrenk and for further utilising them as a promising anthocyanin pigment source. This research enriched the chemical information of B. heteropoda Schrenk. PMID:25415473

Sun, Li-Li; Gao, Wan; Zhang, Meng-Meng; Li, Cheng; Wang, Ai-Guo; Su, Ya-Lun; Ji, Teng-Fei

2014-01-01

278

WINDOWS OF OPPORTUNITY: HISTORICAL AND ECOLOGICAL CONTROLS ON BERBERIS THUNBERGII INVASIONS  

Microsoft Academic Search

Attempts to determine characteristics that render habitats invasible to nonnative species have met with limited success. This may be because most studies focus on modern habitat conditions and do not consider invasibility in the context of a historically dynamic landscape in which both the abundance of a species and the invasibility of a site may change. We surveyed 159 currently

Brian G. DeGasperis; Glenn Motzkin

2007-01-01

279

Japanese Barberry (Berberis thunbergii) in Forest Understory: Leaf and Whole Plant Responses to Nitrogen Availability  

Microsoft Academic Search

We investigated whether Japanese barberry in forest understory responded to N availability through physiological adjustments at the leaf level or growth components at the plant level. Barberry increased foliar N, photosynthesis at ambient and saturating light and specific leaf area in proportion to N availability. Barberry relative production rate increased with foliar N, leaf biomass, specific leaf area and total

ROBIN A. HARRINGTON; JAMES H. FOWNES; TIMOTHY M. CASSIDY

2004-01-01

280

New Hampshire Department of Agriculture  

E-print Network

Acer platanoides Tree of Heaven Ailanthus altissima Garlic Mustard Alliaria petiolata Japanese Barberry Berberis thunbergii European Barberry Berberis vulgaris Oriental Bittersweet Celastrus orbiculatus Spotted

New Hampshire, University of

281

Phytotoxic compounds from roots of Centaurea diffusa Lam.  

PubMed Central

An extract of roots of Centaurea diffusa (diffuse knapweed) yielded caryophyllene oxide and linoleic acid which were shown to be phytotoxic. Also isolated were germacrene B, a previously-known phytotoxin as well as the inactive polyene aplotaxene. A combination of these compounds, if transferred to the soil, could be one factor in the invasive behavior of this weed. Contrary to a literature report, 8-hydroxyquinoline was not detected in root exudates of in vitro grown C. diffusa nor could it be identified in the root extract. However, a recent report from a different group maintains that 8-hydroxyquinoline can be released from roots of C. diffusa following a diurnal rhythm. PMID:19568334

Quintana, Naira; El Kassis, Elie G; Stermitz, Frank R

2009-01-01

282

Radical scavenging potential of phenolics from Bryophyllum pinnatum (LAM.) OKEN.  

PubMed

Optimization of the extraction process of phenolics from Bryophyllum pinnatum was carried out using response-surface methodology (RSM). The effect of different variables such as ratio of solvents, plant material/solvent ratio, extraction time, and temperature were investigated. An optimal phenolics yield of 7.952 mg/g gallic acid equivalence (GAE) was achieved at reduced levels of methanol/water ratio (1:1, v/v). During optimization, the product yield was enhanced by ?2-fold at reduced extraction solvent (methanol/water) up to 37%. Validation of the RSM model for extraction of total phenolic content (TPC) was confirmed by high-performance liquid chromatography (HPLC) analysis. The obtained experimental values were in good agreement with the predicted values, thereby indicating the appropriateness of the model generated. Phenolic extracts from B. pinnatum were further examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods for determining the radical scavenging activities. EC(50) values of B. pinnatum extracts (BPEs) obtained by these methods were in accordance with the amount of phenolics present in the extract. Significant correlation was found between total phenolic content and antioxidant activities (p < 0.05). PMID:21660869

Gupta, Suneel; Banerjee, Rintu

2011-01-01

283

Target Material PHOTORESIST Marvell Equipment Name LAM6  

E-print Network

no no no Endpoint yes yes no no yes no no no no yes yes yes yes yes no yes Base Pressure Pressure 250 mT 100 mT 4 mT 125 Torr 10 mT 80 mT 27 m clamp electrostatic electrostatic arm elec trostatic electrostatic electrostatic platen platen

Healy, Kevin Edward

284

What Are the Signs and Symptoms of LAM?  

MedlinePLUS

... Enlarged lymph nodes. These usually occur in the abdomen or the chest. Very rarely, enlarged lymph nodes may occur in locations where they can be felt, such as the neck or under the arms. Abdominal swelling, sometimes with pain. Swelling in the legs, ...

285

Ecology and Management of Diffuse Knapweed (Centaurea diffusa Lam.)  

Microsoft Academic Search

A close relative of spotted knapweed in the Asteraceae taxonomic family, diffuse knapweed is typically biennial, reproducing exclusively by seed. It forms a rosette with a central crown and tap root in the juvenile stage and a single upright stem one to three feet (0.3 to 0.9 m) tall with numerous spreading branches at maturity. White (occasionally purple) flowers are

Jim Jacobs; Sharlene Sing

286

Moringa oleifera Lam.: Protease activity against blood coagulation cascade  

PubMed Central

Background: The present study evaluated the protease activity of aqueous extracts of Moringa oleifera (Moringaceae) leaf (MOL) and root (MOR). Materials and Methods: Protease activity was assayed using casein, human plasma clot and human fibrinogen as substrates. Results: Caseinolytic activity of MOL was significantly higher (P ? 0.05) than that of MOR. Similar observations were found in case of human plasma clot hydrolyzing activity, wherein MOL caused significantly higher (P ? 0.05) plasma clot hydrolysis than MOR. Zymographic techniques were used to detect proteolytic enzymes following electrophoretic separation in gels. Further, both the extracts exhibited significant procoagulant activity as reflected by a significant decrease (P ? 0.05) in recalcification time, accompanied by fibrinogenolytic and fibrinolytic activities; clotting time was decreased from 180 ± 10 sec to 119 ± 8 sec and 143 ± 10 sec by MOL and MOR, respectively, at a concentration of 2.5 mg/mL. Fibrinogenolytic (human fibrinogen) and fibrinolytic activity (human plasma clot) was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), plate method and colorimetric method. Zymographic profile indicated that both the extracts exerted their procoagulant activity by selectively hydrolyzing A? and B? subunits of fibrinogen to form fibrin clot, thereby exhibiting fibrinogenolytic activity. However, prolonged incubation resulted in degradation of the formed fibrin clot, suggesting fibrinolytic like activity. Conclusions: These findings support the traditional usage of M. oleifera extracts for wound healing. PMID:22224061

Satish, A; Sairam, Sudha; Ahmed, Faiyaz; Urooj, Asna

2012-01-01

287

Hemostatic, antibacterial biopolymers from Acacia arabica (Lam.) Willd. and Moringa oleifera (Lam.) as potential wound dressing materials.  

PubMed

Acacia arabica and Moringa oleifera are credited with a number of medicinal properties. Traditionally gum of Acacia plant is used in the treatment of skin disorders to soothe skin rashes, soreness, inflammation and burns while Moringa seed extracts are known to have antibacterial activity. In the present study the potential of the polymeric component of aqueous extracts of gum acacia (GA) and the seeds of M. oleifera (MSP) in wound management was evaluated. The results revealed that both biopolymers were hemostatic and hasten blood coagulation. They showed shortening of activated partial thromboplastin time and prothrombin time and were non-cytotoxic in nature. Both showed antibacterial activity against organisms known to be involved in wound infections with MIC ranging from 500-600 microg mL(-1) for GA and 300-700 microg mL(-1) for MSP. They were biodegradable and exhibited water absorption capacity in the range of 415 to 935%. The hemostatic character coupled to these properties envisions their potential in preparation of dressings for bleeding and profusely exuding wounds. The biopolymers have been further analysed for their composition by Gas chromatography. PMID:24266104

Bhatnagar, Monica; Parwani, Laxmi; Sharma, Vinay; Ganguli, Jhuma; Bhatnagar, Ashish

2013-10-01

288

Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine.  

PubMed

In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, and Microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover, antileishmanial and cytotoxicity activity of B. vulgaris and berberine against promastigotes of Leishmania major and Leishmania tropica were evaluated by colorimetric MTT assay. The findings indicated that the various extracts of B. vulgaris particularly berberine showed high potential antidermatophytic against pathogenic dermatophytes tested with MIC values varying from 0.125 to >4?mg/mL. The results revealed that B. vulgaris extracts as well as berberine were effective in inhibiting L. major and L. tropica promastigotes growth in a dose-dependent manner with IC50 (50% inhibitory concentration) values varying from 2.1 to 26.6? ? g/mL. Moreover, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages with CC50 (cytotoxicity concentration for 50% of cells) values varying from 27.3 to 362.6? ? g/mL. Results of this investigation were the first step in the search for new antidermatophytic and antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in animal models as well as volunteer human subjects. PMID:24977052

Mahmoudvand, Hossein; Ayatollahi Mousavi, Seyyed Amin; Sepahvand, Asghar; Sharififar, Fariba; Ezatpour, Behrouz; Gorohi, Fatemeh; Saedi Dezaki, Ebrahim; Jahanbakhsh, Sareh

2014-01-01

289

Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids  

Microsoft Academic Search

Extracts obtained from the roots of Berberidaceae species have been used in Eastern and Bulgarian folk medicine in rheumatic and other chronic inflammatory disorders. The investigations of the chemical composition and immunological properties show that their activity is mainly due to the alkaloid constituents. In the present study the anti-inflammatory properties of total ethanol extract (TEE), three alkaloid fractions, a

Nina Ivanovska; Stefan Philipov

1996-01-01

290

Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine  

PubMed Central

In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, and Microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover, antileishmanial and cytotoxicity activity of B. vulgaris and berberine against promastigotes of Leishmania major and Leishmania tropica were evaluated by colorimetric MTT assay. The findings indicated that the various extracts of B. vulgaris particularly berberine showed high potential antidermatophytic against pathogenic dermatophytes tested with MIC values varying from 0.125 to >4?mg/mL. The results revealed that B. vulgaris extracts as well as berberine were effective in inhibiting L. major and L. tropica promastigotes growth in a dose-dependent manner with IC50 (50% inhibitory concentration) values varying from 2.1 to 26.6??g/mL. Moreover, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages with CC50 (cytotoxicity concentration for 50% of cells) values varying from 27.3 to 362.6??g/mL. Results of this investigation were the first step in the search for new antidermatophytic and antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in animal models as well as volunteer human subjects. PMID:24977052

Mahmoudvand, Hossein; Ayatollahi Mousavi, Seyyed Amin; Sepahvand, Asghar; Sharififar, Fariba; Ezatpour, Behrouz; Gorohi, Fatemeh; Saedi Dezaki, Ebrahim; Jahanbakhsh, Sareh

2014-01-01

291

Linking seed dispersal, germination and seedling recruitment in the invasive species Berberis darwinii (Darwin’s barberry)  

Microsoft Academic Search

Seedling recruitment is a multi-phased process involving seed production, dispersal, germination, seedling establishment and\\u000a subsequent survival. Understanding the factors that determine success at each stage of this process is of particular interest\\u000a to scientists and managers seeking to understand how invasive species spread and persist, and identify critical stages for\\u000a management. To understand the factors and processes influencing recruitment of

Kate G. McAlpine; Linley K. Jesson

2008-01-01

292

[The use of barberry berries in human nutrition].  

PubMed

The contents of carbohydrates, organic acids, some vitamin, poliphenolic compounds, pectin tannin, mineral elements, in berberis vulgaris are presented. Describle utilize berberis in traditional medicine and dietetica are also outlined. PMID:12968305

Pozniakovski?, V M; Golub, O V; Popova, D G; Kovalevskaia, I N

2003-01-01

293

Biological Invasions 1: 189201, 1999. 1999 Kluwer Academic Publishers. Printed in the Netherlands.  

E-print Network

of Japanese barberry was encouraged as an ornamental shrub replacing the common barberry (Berberis vulgaris: the common barberry (Berberis vulgaris L.) and Japanese barberry. These are closely related species in the Netherlands. The invasion ecology of Japanese barberry (Berberis thunbergii) in the New England landscape John

Silander Jr., John A.

294

New Phytologist (2002) 154: 717730 www.newphytologist.com 717 Blackwell Science, Ltd  

E-print Network

Insect-mediated reproduction of systemic infections by Puccinia arrhenatheri on Berberis vulgaris Andreas. 107, CH-8008 Zürich, Switzerland Summary · Witches' brooms on Berberis vulgaris are induced.naef@ipw.agrl.ethz.ch Received: 26 October 2001 Accepted: 21 January 2002 Introduction Berberis vulgaris L., or common Barberry

Oregon, University of

295

Effect of Dietary Dried Berberis Vulgaris Fruit and Enzyme on Some Blood Parameters of Laying Hens Fed Wheat-Soybean Based Diets  

Microsoft Academic Search

2 Abstract: A study was conducted to evaluate dried berberry fruit (DBF) and enzyme on some blood parameters of laying hens. In a 5 * 2 completely randomized block design with factorial arrangement and 4 blocks as replicate, 5 levels of DBF (0.0, 0.5, 1.0, 1.5, and 2%) and 2 levels of NSP-degrading enzyme (0.0, and 0.05%) in wheat-soybean based

2006-01-01

296

Clinical role of a fixed combination of standardized Berberis aristata and Silybum marianum extracts in diabetic and hypercholesterolemic patients intolerant to statins  

PubMed Central

Background Statin intolerance is a medical condition often leading patients to nonadherence to the prescribed therapy or to a relevant reduction of the statin dosage. Both situations determine a totally or partially uncontrolled lipid profile, and these conditions unquestionably increase the risk of cardiovascular events. Methods We enrolled hypercholesterolemic, type 2 diabetic patients complaining of intolerance to statins. Some of them had reduced the statin dose ‘until the disappearance of symptoms’; others had opted for treatment with ezetimibe; and yet others were not undergoing any treatment at all. All patients of the three groups were then given a fixed combination of berberine and silymarin (Berberol®), known from previous papers to be able to control both lipidic and glycemic profiles. Results The tested product both as a single therapy and as add-on therapy to low-dose statin or to ezetimibe reduced triglycerides, low-density lipoprotein cholesterol, fasting blood glucose, and glycosylated hemoglobin in a significant manner without inducing toxicity conditions that might be somehow ascribed to a statin-intolerant condition. Conclusion Our study demonstrates that use of Berberol®, administered as a single or add-on therapy in statin-intolerant subjects affected by diabetes and hypercholesterolemia is a safe and effective tool capable of improving the patients’ lipidic and glycemic profiles.

Di Pierro, Francesco; Bellone, Iaele; Rapacioli, Giuliana; Putignano, Pietro

2015-01-01

297

Seasonal variation of temperature response of respiration in invasive Berberis thunbergii (Japanese barberry) and two co-occurring native understory shrubs in a northeastern US deciduous forest  

Microsoft Academic Search

In the understory of a closed forest, plant growth is limited by light availability, and early leafing is proposed to be an\\u000a important mechanism of plant invasion by providing a spring C “subsidy” when high light is available. However, studies on\\u000a respiration, another important process determining plant net C gain, are rare in understory invasive plants. In this study,\\u000a leaf

Cheng-Yuan Xu; W. S. F. Schuster; Kevin L. Griffin

2007-01-01

298

Analysis of isoquinoline alkaloids in medicinal plants by capillary electrophoresis-mass spectrometry.  

PubMed

The technique of capillary electrophoresis - mass spectrometry (CE-MS) was applied for determination of isoquinoline alkaloids in crude methanolic extracts of medicinal plants. For the CE separations ammonium formate buffer solutions (70 or 100 mM, pH 3.0 or 4.0) containing 10% methanol or 20-60% acetonitrile as additives were used. The applied voltage was 25 kV, the thermostating temperature was kept constant at 25 degrees C. Coupling with the mass spectrometer was performed via an atmospherical pressure ionization (API) interface and the electrospray ionization technique (ESI). As sheath liquid 5 mM formic acid in acetonitrile at a flow rate of 3 microL/min was used. The spray voltage was 4.5 kV and the temperature of the heated capillary was chosen to be 200 degrees C. Detection in the positive ionization mode resulted in mass spectra showing either the molecular ions [M]+ or the protonated molecular ions [M+H]+. The presented method allows detection and identification of isoquinoline alkaloids in crude methanolic extracts of medicinal plants as Eschscholzia californica CHAM. (Papaveraceae), Hydrastis canadensis L. (Ranunculaceae), Berberis vulgaris L. (Berberidaceae), Jateorhiza palmata (LAM.) MIERS (Menispermaceae) and Chelidonium majus L. (Papaveraceae). PMID:9870408

Sturm, S; Stuppner, H

1998-11-01

299

In vitro callus induction and estimation of plumbagin content from Plumbago auriculata Lam.  

PubMed

The medicinal plant Plumbago contains a very potent secondary metabolite, plumbagin having many therapeutic properties. Callus culture was induced using explants, leaf, stem and shoot apex, from P. auriculata. Murashige and Skoog media fortified with various growth hormones like NAA, IAA, IBA and 2, 4-D individually and in various combinations were checked for callus induction. Among the growth hormones used, 1 mg/L 2, 4-D showed best callusing. The hormonal combinations of 1 mg/L IAA and 1.5 mg/L NAA in the media exhibited best callus induction using stem internode as an explant. Plumbagin content from root, stem, leaf and callus was analyzed by using thin layer chromatographic technique. The callus derived from stem showed comparable plumbagin content to the in vivo plant parts. Quantitative spectrophotometric analysis of plumbagin from plant samples and callus indicated that plumbagin content was maximum in roots which was followed by callus, stem and leaf samples respectively. Generation of in vitro sources for p!umbagin, for therapeutic applications will serve as a continuous supply and will contribute to preserve the natural plant recourses. PMID:25434108

Deshpande, Jyoti; Labade, Dinesh; Shankar, Kalaiselvi; Kata, Navin; Chaudhari, Manoj; Wani, Minal; Khetmalas, Madhukar

2014-11-01

300

Identification, distribution, and control of triasulfuron-resistant Italian ryegrass (Lolium multiflorum Lam.) in Central Texas  

E-print Network

et al. 1994). The first report of herbicide resistance was that of triazine resistant common groundsel (Senecio vulgaris L. ) in 1970 (Heap 1997; Holt and Lebaron 1990; Ryan 1970). Since that time, over 150 herbicide resistant weed species...

Tucker, Kevin Patrick

2002-01-01

301

Recognizing Faces Showing Expressions Yaser Yacoob, HeungMan Lam and Larry S. Davis  

E-print Network

expression. Some ap­ proaches are biased towards recognizing males over females, or particular race and skin and graph matching, attempt to determine the utility of these two popular approaches to face recognition approaches. The eigenface approach pro­ posed in [TUR91] and the feature­graph based approach [MAN91] have

Yacoob, Yaser

302

VizieR Online Data Catalog: Spectroscopy around lam Ori and sig Ori (Maxted+, 2008)  

NASA Astrophysics Data System (ADS)

Radial velocities and spectral indices for 218 stars and brown dwarfs around {sigma} and {lambda} Orionis. Observations were obtained with the FLAMES/GIRAFFE multi-object spectrograph on ESO's VLT UT2 (Kueyen) telescope (programme ID 076.C_145). The equivalent width of the NaI 8200{AA} and the strength of the TiO 8442{AA} spectral features were measured from the median average spectrum. Radial velocities were measured by cross-correlation against a template spectrum of the brown dwarf star USco CTIO 055 at multiple epochs. (3 data files).

Maxted, P. F. L.; Jeffries, R. D.; Oliveira, J. M.; Naylor, T.; Jackson, R. J.

2008-05-01

303

Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam  

Microsoft Academic Search

Moringa oleifera is an important multipurpose tree introduced to Africa from India at the turn of this century. Despite limited knowledge of the levels of genetic diversity and relatedness of introduced populations, their utilization as a source of seed for planting is widespread. In order to facilitate reasoned scientific decisions on its management and conservation and prepare for a selective

G. M. MULUVI; J. I. SPRENT; N. SORANZO; J. PROVAN; D. ODEE; G. FOLKARD; J. W. McNICOL; W. POWELL

1999-01-01

304

Removal of Zn(II) ions from aqueous solution using Moringa oleifera Lam. (horseradish tree) biomass  

Microsoft Academic Search

The removal of Zn(II) ions from aqueous solution using pure and chemically pretreated biomass of Moringa oleifera was investigated at 30±1°C in this study. The experimental results explored that the maximum pH (pHmax) for efficient sorption of Zn(II) was 7±0.1 at which evaluated biosorbent dosage and biosorbent particle size, were 0.5g\\/L, <0.255mm, respectively. The cellular Zn(II) concentration increased with the

Haq Nawaz Bhatti; Beenish Mumtaz; Muhammad Asif Hanif; Raziya Nadeem

2007-01-01

305

Medicinal potential of Morella serata (Lam.) Killick (Myricaceae) root extracts: biological and pharmacological activities  

PubMed Central

Background Morella serata is a South African medicinal plant used in the treatment of microbial infections and to enhance male sexual performance. There is dearth of information in scientific literature on its efficacy and safety. Methods In the present study, the root extracts were investigated for the phytochemicals that may be present the antibacterial, anticandida activity using 96 wells microtitre plate method and cytotoxicity using brine shrimp (Artemia salina) lethality assay. Results The qualitative phytochemical screening revealed the presence of tannins, saponins, flavonoids, terpenoids and steroids. All the extracts including water inhibited both Gram positive and Gram negative bacteria strains at minimum inhibitory concentrations (MIC) ranging from 0.09 – 6.25 mgmL-1. The best activity was observed in the acetone extract inhibiting all the bacteria tested at MIC range of 0.09 – 0.78 mgmL-1 except Shigella flexneri KZN that was inhibited at 1.56 mgmL-1. Similarly, all the extracts suppressed the growth of all Candida species and Trichophyton mucoides at MIC ranging from 0.13 – 3.13 mgmL-1. The cytotoxicity assay revealed potent cytotoxic potential of M. serata methanol and ethanol root extracts by displaying LC50 of 0.26 and 0.18 ?gmL-1 respectively. Conclusion The results obtained from the present study indicated broad spectrum antimicrobial activity and justifies the use of the plant in the treatment of infectious diseases. Also the species could be a good natural source of antitumor compounds considering its lethality against brine shrimp nauplii. PMID:23829770

2013-01-01

306

Light effects on leaf development and photosynthetic capacity of Hydrocotyle bonariensis Lam  

Microsoft Academic Search

Rates of net CO2 uptake were examined in developing leaves of Hydrocotyle bonariensis. Leaves that developed under high photosynthetically active radiation (48 mol m-2 day-1 PAR) were smaller, thicker, and reached maximum size sooner than did leaves that developed under low PAR (4.8 mol m-2 day-1). Maximum net CO2 uptake rates were reached after 5 to 6 days expansion for

David J. Longstreth; Terry L. Hartsock; Park S. Nobel

1981-01-01

307

In vitro toxicity, antiplatelet and acetylcholinesterase inhibition of Buddleja thyrsoides Lam. leaves.  

PubMed

Alzheimer's disease (AD) is a neurodegenerative disorder resulting in impaired memory and behaviour of remarkable socio-economic impact. A decrease in cholinergic activity is a key event in the biochemical of AD. Buddleja thyrsoides is a plant widely distributed in Southern parts of South America. In Brazilian traditional medicine, the infusion of its leaves and flowers is used for the treatment of bronchitis and cough. Crude ethanolic (70%) extract and fractions (dichloromethane, ethyl acetate and n-butanolic) were investigated regarding their toxicities in vitro and antiplatelet action. The enzyme acetylcholinesterase inhibition was evaluated to study the crude extract. The crude extract and fractions were evaluated by means of Brine Shrimp Lethality test and they showed low activities with LC(50) values 1698, 2818, 2187 and 3672?µg?mL(-1) for dichloromethane, ethyl acetate, n-butanolic fractions and crude extract, respectively. Buddleja thyrsoides presented great antiplatelet action. The IC(50) values obtained for crude extract and dichloromethane, ethyl acetate and n-butanolic fractions were 361.29, 354.23, 368.75 and 344.30, respectively, while the IC(50) for the standard AAS was 257.01?µg?mL(-1). The crude extract showed an inhibition of 22.8% of the acetylcholinesterase enzyme in 24?h. PMID:22181835

Mahlke, Janaína Dorneles; Boligon, Aline Augusti; Machado, Michel Mansur; Athayde, Margareth Linde

2012-01-01

308

Cytogenetic studies of Lolium multiflorum Lam., Festuca arundinacea Schreb., their hybrids and amphidiploids  

Microsoft Academic Search

Plant breeders intercross Lolium multiflorum and Festuca arundinacea with the purpose of obtaining hybrids which combine agronomically interesting characters of the parent species. The end result can be an amphidiploid, or the transfer of a limited number of genes from one species to the other. Especially in the first case, meiotic regularity often is a bottle neck. In the present

G. Kleijer

1982-01-01

309

Bioremediation of Turbid Surface Water Using Seed Extract from the Moringa oleifera Lam. (Drumstick) Tree.  

PubMed

An indigenous water treatment method uses Moringa oleifera seeds in the form of a crude water-soluble extract in suspension, resulting in an effective natural clarification agent for highly turbid and untreated pathogenic surface water. Efficient reduction (80.0% to 99.5%) of high turbidity produces an aesthetically clear supernatant, concurrently accompanied by 90.00% to 99.99% (1 to 4 log) bacterial reduction. Application of this low-cost Moringa oleifera protocol is recommended for water treatment where rural and peri-urban people living in extreme poverty are presently drinking highly turbid and microbiologically contaminated water. Curr. Protoc. Microbiol. 33:1G.1-1G.8. © 2014 by John Wiley & Sons, Inc. PMID:24789599

Lea, Michael

2014-01-01

310

Physio-anatomic aspects on the initial growth of Guazuma ulmifolia Lam. seedlings.  

PubMed

This paper aimed to evaluate the initial growth of "mutambo" seedlings in different conditions of light intensity and treatments with gibberellic acid (GA). The seedlings were kept under full sun and 50% of shading. Sixty days after the emergence, seedlings were sprayed with: 1) 100 mg L(-1) GA(3); 2) 200 mg L(-1) GA(3); 3) control. At the end of the appraisals, seedlings height under 50% of shading was compared to the height that were growing under full sun with 200 mg L(-1) GA. Stem diameter was lower under shading. Leaf area did not vary among the treatments, but the root system growth was higher under full sun and did not vary among GA levels. The number of stomata, trichomes and epidermal cells on adaxial and abaxial sides was higher under full sun. Total dry masses of leaf and root were highe runder full sun and with 200 mg L(-1) GA application. "Mutambo" seedlings presented a higher initial growth under full sun, although with a lower height, diameter, and lenght of the largest root and total dry masses of leaf and root were higher. A concentration with 200 mg L(-1) promoted a higher growth. PMID:21670888

Scalon, Silvana P Q; Pereira, Heloisa H G; Glaeser, Daniele F; Silva, Jocemar J; Betoni, Roseli; Mussury, Rosilda M

2011-06-01

311

Progress with extreme adaptive optics test bench for ELT at LAM  

Microsoft Academic Search

Direct detection of exo-planets from the ground will become a reality with the advent of a new class of extreme-adaptive optics instruments that will come on-line within the next few years on 8-10 meters class telescopes. One major technical challenge in reaching the requisite high contrast at small angles is the sensing and control of wave front errors which becomes

Maud Langlois; Celine Pasanau; Brice Leroux; Gil Moretto; Kacem El Hadi; Didier Rabaud

2008-01-01

312

Leaf growth, senescence and decomposition of Juncus maritimus Lam. in a coastal Mediterranean marsh  

Microsoft Academic Search

In this study, the growth, senescence, leaf loss and nutrient dynamics of Juncus maritimus were followed to examine litter decay in a Mediterranean coastal marsh. Decomposition was studied in dead leaves still attached to the plant and in leaves placed in litterbags (detached leaves\\/litter) on the sediment surface. The dynamics of fungi, meiofauna and epiphytes associated with detached litter were

Margarita Menéndez

2008-01-01

313

Analysis of genetic diversity in Chinese sweetpotato [Ipomoea batatas (L.) Lam.] germplasm using DNA amplification fingerprinting.  

PubMed

The genetic diversity and evolutionary relationships in a representative sample of Chinese sweetpotato collection were assessed using the DNA amplification fingerprinting approach. DNA fingerprint profiles were developed for all 42 accessions tested. There were 19-26 bands for each accession and an average of 20.7 such bands were polymorphic. Chinese sweetpotato germplasm tested exhibited a high degree of genetic diversity. Phenetic analysis revealed five major clusters with the following components: (1) landraces from Guangdong Province, (2) landraces from Fujian province, (3) Chinese cultivars, (4) those closely related to Japanese sweetpotato cultivars, and (5) those closely related to the US sweetpotato cv. Nancy Hall. The genetic association observed between accessions was largely consistent with the known pedigree records. The DNA amplification fingerprinting may provide reliable insights into the domestication history of the sweetpotato crop and may be useful in germplasm enhancement. PMID:12296360

Wang, J; He, G; Prakash, C S; Lu, S

1998-01-01

314

Integration and test activities for the SUMIRE prime focus spectrograph at LAM  

NASA Astrophysics Data System (ADS)

The Prime Focus Spectrograph (PFS) of the Subaru Measurement of Images and Redshifts (SuMIRe) project for Subaru telescope consists in four identical spectrographs feed by 600 fibers each. Each spectrograph is composed by an optical entrance unit that creates a collimated beam and distributes the light to three channels, two visible and one near infrared. We present here the integration process of the first spectrograph channel. The verification requirements, the specific integration requirements and the product tree are the main drivers from the top plan for the Assembly Integration and Test (AIT) development process. We then present the AIT flow-down, the details for the AIT processes as well as opto-mechanical alignment procedures and tests setup. In parallel, we are developing and validating dedicated tools to secure and facilitate the AIT activities, as we have to assemble eight visible cameras, integrate and align four fiber slits, integrate and align the components of four spectrographs.

Madec, F.; Jaquet, Marc; Pascal, Sandrine; Bozier, A.; Le Mignant, David; Vives, S.; Ferrand, D.; Pegot-Ogier, T.; Arthaud, G.; Golebiowski, M.; Sugai, H.; Tamura, N.; Gunn, J.; Smee, S.; Oliveira, L.

2014-07-01

315

Establishment of bermudagrass (Cynodon dactylon (L.) Pers.) seeded with annual ryegrass (Lolium multiflorum Lam.  

E-print Network

companion grass without some cultural management when a bermudagrass cover is desired. 40 LITERATURE CITED Ahspapanek, D. C. 1962, Ecological studies on plant inhibition by Solanum rostratum. (Unpublished) Ph. D. Thesis, Oklahoma University, Norman...

Bowmer, William Jackson

1967-01-01

316

Wound healing potential of ethanolic extract of Kalanchoe pinnata Lam. leaf--a preliminary study.  

PubMed

The extract of K. pinnata was evaluated for its wound healing activity by using excision wound model in rats. On day 11, animals treated with the ethanolic leaf extract exhibited 86.33% reduction in the wound area, compared to petroleum jelly treated control (69.36%) and the mupirocin treated standard (85.49%). The hydroxyproline content of extract treated animals was higher, as compared to control and the standard groups. Histological analysis was also consistent with the proposal that K. pinnata leaf extract exhibits significant wound healing potential. The increased rate of wound contraction and hydroxyproline content in the extract treated animals supports the claims made by traditional healers of the benefits obtained from the medicinal use of K. pinnata. PMID:20882759

Nayak, B Shivananda; Marshall, Julien R; Isitor, Godwin

2010-06-01

317

Towards the Generalization of Game-Based Learning: Integrating Educational Video Games in LAMS  

Microsoft Academic Search

In e-Learning the interest on the use of new kinds of highly-interactive content is increasing. In this regard video games are one of the media that is gaining momentum because of their high potential for enhancing both students' motivation and learning outcomes. Nevertheless, the actual adoption of games in the educational system is scarce. In this paper we identify different

Ángel del Blanco; Javier Torrente; Pablo Moreno-Ger; Baltasar Fernández-Manjón

2010-01-01

318

Evaluation of cytotoxicity of Moringa oleifera Lam. callus and leaf extracts on Hela cells  

PubMed Central

Background: There are considerable attempts worldwide on herbal and traditional compounds to validate their use as anti-cancer drugs. Plants from Moringaceae family including Moringa oleifera possess several activities such as antitumor effect on tumor cell lines. In this study we sought to determine if callus and leaf extracts of M. oleifera possess any cytotoxicity. Materials and Methods: Ethanol-water (70-30) extracts of callus and leaf of M. oleifera were prepared by maceration method. The amount of phenolic compounds of the extracts was determined by Folin Ciocalteu method. The cytotoxicity of the extracts against Hela tumor cells was carried out using MTT assay. Briefly, cells were seeded in microplates and different concentrations of the extract were added. Cells were incubated for 48 h and their viability was evaluated by addition of tetrazolium salt solution. After 3 h medium was aspirated, dimethyl sulfoxide was added and absorbance was determined at 540 nm with an ELISA plate reader. Cytotoxicity was considered when more than 50% reduction on cell survival was observed. Results: Callus and leaf extracts of M. oleifera significantly decreased the viability of Hela cells in a concentration-dependent manner. However, leaf extract of M. oleifera were more potent than that of callus extract. Conclusion: As the content of phenolic compounds of leaf extract was higher than that of callus extract, it can be concluded that phenolic compounds are involved in the cytotoxicity of M. oleifera. PMID:25337524

Jafarain, Abbas; Asghari, Gholamreza; Ghassami, Erfaneh

2014-01-01

319

Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds.  

PubMed

Seeds of the tropical tree Moringa oleifera contain small storage proteins able to flocculate particles in suspension in water. The cDNA encoding one of these flocculent proteins, MO(2.1), was cloned and the recombinant protein was expressed in Escherichia coli. The flocculent activity of the purified recombinant MO(2.1)was assayed on clays and bacteria using light and confocal microscopy and GFP-overexpressing bacteria. We show that MO(2.1)is able to aggregate montmorillonite clay particles as well as gram-positive and gram-negative bacteria. We discuss the use of recombinant proteins to study flocculating properties and improve water purification processes. PMID:12382051

Broin, M; Santaella, C; Cuine, S; Kokou, K; Peltier, G; Joët, T

2002-10-01

320

Antitrypanosomal Property of Some Extracts of Different Parts of Moringa oleifera, Lam  

Microsoft Academic Search

Different extracts of Moringa oleifera parts were evaluated for their anti-trypanosomal activity and broad phytochemical classes. Alkaloids, resins and saponins were detected. In vitro, the petroleum ether extract of the root bark, chloroform extract of the stem bark, methanol extracts of the stem and the aqueous extracts of all parts were active at 4 and 2 mg\\/ ml doses. The

Sunday E. Atawodi; Hassana Shehu

321

Antioxidant, antimicrobial and phytochemical variations in thirteen Moringa oleifera Lam. cultivars.  

PubMed

A study was undertaken to assess variation in antioxidant, antimicrobial and phytochemical properties of thirteen Moringa oleifera cultivars obtained from different locations across the globe. Standard antioxidant methods including the DPPH scavenging, ferric reducing power (FRAP) and ?-carotene-linoleic acid model were used to evaluate the activity. Variation in the antioxidant activity was observed, with TOT4951 from Thailand being the most active, with activity five times higher than that of ascorbic acid (reference compound). A different trend was observed for the activity in the FRAP and ?-carotene-linoleic acid assays. Antimicrobial activity was tested against Gram-positive (Staphylococcus aureus) and Gram-negative (Klebsiella pneumoniae) strains using the microdilution method. Acetone extracts of all cultivars exhibited good antibacterial activity against K. pneumoniae (MIC values of 0.78 mg/mL). The remaining extracts exhibited weak activity against the two microorganisms. For the antifungal activity, all the extracts exhibited low activity. Variations were observed in the total phenolic and flavonoid contents. Cultivars TOT5169 (Thailand) and SH (South Africa) exhibited highest amounts of total phenolic compounds while TOT5028 (Thailand) exhibited the lowest amounts of five times lower than the highest. The information offer an understanding on variations between cultivars from different geographical locations and is important in the search for antioxidant supplementation and anti-ageing products. PMID:25045889

Ndhlala, Ashwell R; Mulaudzi, Rofhiwa; Ncube, Bhekumthetho; Abdelgadir, Hafiz A; du Plooy, Christian P; Van Staden, Johannes

2014-01-01

322

Quality of cooked ground buffalo meat treated with the crude extracts of Moringa oleifera (Lam.) leaves  

Microsoft Academic Search

The study was conducted to evaluate the physico-chemical, microbial and organoleptic qualities of cooked ground buffalo meat\\u000a (GBM), treated with, 1, 1.5 and 2% levels of aqueous solution of crude extract of drumstick (Moringa oleifera) leaves. The\\u000a meat samples treated with 1.5% crude extract of drumstick leaves significantly (P?

Suchandra Hazra; Subhasish Biswas; Debasish Bhattacharyya; Sudip Kumar Das; Anupam Khan

323

Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds  

Microsoft Academic Search

Seeds of the tropical tree Moringa oleifera contain small storage proteins able to flocculate particles in suspension in water. The cDNA encoding one of these flocculent proteins, MO2.1, was cloned and the recombinant protein was expressed in Escherichia coli. The flocculent activity of the purified recombinant MO2.1 was assayed on clays and bacteria using light and confocal microscopy and GFP-overexpressing

M. Broin; C. Santaella; S. Cuine; K. Kokou; G. Peltier; T. Joët

2002-01-01

324

Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir  

Microsoft Academic Search

This report describes in vitro micropropagation of Boscia senegalensis, so-called famine foods, that helped the people in Darfur and Kordofan, Sudan survive during the 1984–1985 famine. Four types\\u000a of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1–5\\u000a mg\\/L 6-benzyladenine (BA). The highest number of shoots per explant (14.3±0.9) was achieved

Mutasim M. Khalafalla; Hussien M. Daffalla; Eltayb Abdellatef; Elsadig Agabna; Hany A. El-Shemy

2011-01-01

325

Gastroprotective effect of aqueous extract and mucilage from Bryophyllum pinnatum (Lam.) Kurz  

PubMed Central

Context: Bryophyllum pinnatum is used as traditional medicine in India, Africa, Tropical America and China for treatment of various diseases. B. pinnatum contains different groups of phytoconstituents viz., flavonoid, terpenoids, alkaloid, phenolic compounds. Aim: The present study was carried out to evaluate the gastroprotective activity of B. pinnatum whole plant aqueous extract, and mucilage (MUC) isolated from the whole plant against ethanol induced gastric ulcer. Materials and Methods: Pretreatment of rats with aqueous extract at dose level of 500 and 750 mg/kg b.w., MUC at 500 mg/kg dose level and standard drug Rabeprazole at dose level of 20 mg/kg b.w. where given for 7 days. Results: The aqueous whole plant extract of B. pinnatum at dose of 750 mg/kg p.o. and MUC at dose of 500 mg/kg p.o. markedly decrease the incidence of ulcers in ethanol induced ulcer rats. In ethanol induced ulcer rats, there was a decrease in the gastric volume, free and total acidity and ulcerative index as compared to the control group. Total carbohydrate content was found to be an increase as compare to control the group. The aqueous whole plant extract of B. pinnatum at dose of 750 mg/kg showed a significant reduction in the above parameters which was comparable to the standard drug rabeprazole (20 mg/kg). B. pinnatum extract and MUC showed protection index 72.69 and 69.65% respectively, whereas standard drug rabeprazole showed protection index 75.49%. Conclusions: Whole plant extracts of B. pinnatum and MUC has potent gastroprotective effect which can be further clinically studied for new drug development. PMID:25593406

Sharma, Abhishek L.; Bhot, Meeta A.; Chandra, Naresh

2014-01-01

326

Seeding rate and seed size as management techniques for ryegrass (Lolium Multiflorum, Lam) in winter wheat  

E-print Network

Higher seeding rates and larger seed sizes could enhance the competitiveness of wheat with ryegrass. Growth room and field research evaluated the effects of wheat seeding rates and seed size in competition with Italian ryegrass. Winter wheat seeds...

Cook, Casey Lee

2005-08-29

327

Longitudinal Assessment of Manic Symptoms (LAMS) Study: Background, Design and Initial Screening Results  

PubMed Central

Objective To describe the design of a longitudinal study of youth with elevated symptoms of mania (ESM) as well as the prevalence and correlates of manic symptoms. Bipolar disorder in youth is serious and is surrounded by controversy about its phenomenology, course and treatment. Yet, there are no longitudinal studies of youth selected only for ESM, the phenomenological hallmark. The study’s objective is to document the rate and sociodemographic correlates of ESM in children attending outpatient psychiatric clinics. Method Parents of 3329 6–12 year old children visiting 10 outpatient clinics were asked to complete the Parent General Behavior Inventory-10 Item Mania Scale (PGBI-10M). Children with PGBI-10M scores ? 12 (ESM+) and a matched sample of screen negatives (ESM?) were invited to enroll in the longitudinal study. Results Most (N=2622, 78.8%) participated. Nonparticipants were slightly younger (M=9.1 years, SD=2.0 versus 9.4 years, SD=2.0; t=4.42, df=3327, p<0.001). Nearly half (43%) were ESM+; these were more likely to be Latino (4.2% versus 2.5%, X2 =5.45, df=1, p=0.02), younger (M=9.3 years, SD=2.0 versus M=9.6 years, SD=1.9, t=3.8, df=2620, p<0.001) and insured by Medicaid (48.4% versus 35.4%, X2 =45.00, df=1, p<0.001). There were no sociodemographic differences between those who did versus did not agree to enroll in the longitudinal portion (ESM+ N=621, 55.2%; ESM? N=503, 44.8%). Four items best discriminated ESM+ from ESM?. These were not the most commonly endorsed but were indicative of behavioral extremes. Conclusions Data suggest ESM+ is not rare in 6 to 12 year olds. ESM+ children show behavioral extremes including rapid mood shifts compared to ESM? children. PMID:21034684

Horwitz, Sarah McCue; Demeter, Christine; Pagano, Maria E.; Youngstrom, Eric A.; Fristad, Mary A.; Arnold, L. Eugene; Birmaher, Boris; Gill, Mary Kay; Axelson, David; Kowatch, Robert A.; Frazier, Thomas W.; Findling, Robert L.

2011-01-01

328

A spatial light modulator for terahertz beams Wai Lam Chan,1  

E-print Network

small voltage levels, at room temperature, with low power consumption and reasonably high switching at their respective array positions. Traditional technologies for SLMs in the optical regime,3,8 which use liquid charge carriers upon voltage bias which in turn changes the loss at the capacitive split gaps

Mittleman, Daniel

329

Antimicrobial and Antioxidant Activities of Bauhinia racemosa Lam. and Chemical Content  

PubMed Central

Methanol 70% extract of Bauhinia racemosa aerial parts was tested for antimicrobial activity against different bacterial and fungal strains and for antioxidant activity and also total content of polyphenols with phytochemical analysis of the extract was determined. The results have showed that the extract has a significant antimicrobial activity, it inhibited the growth of Bacillus subtilis and also it was highly active against Candida albicans suggesting that it can be used in the treatment of fungal infections. The extract has a good antioxidant activity, it has shown high values of the trolox equivalent antioxidant capacity and polyphenol content while it has shown a lower value of oxygen radical absorbance capacity. Phytochemical analysis has shown that it has interesting phytochemical bioconstituents, include flavonoids, tannins and others, and the deep phytochemical study results in the isolation of methyl gallate, gallic, kaempferol, quercetin, querection 3–O–?–rhamnoside, kaempferol 3–O–?–glucoside, myricetin–3–O–?– glucoside, querection–3–O–rutinoside (Rutin). All compounds were identified by different spectroscopic analyses (UV, 1H–NMR, 13C–NMR) and Mass Spectrometry (MS). PMID:25276210

Rashed, Khaled; Butnariu, Monica

2014-01-01

330

Bioremediation of turbid surface water using seed extract from Moringa oleifera Lam. (drumstick) tree.  

PubMed

An indigenous water treatment method uses Moringa oleifera seeds in the form of a water-soluble extract in suspension, resulting in an effective natural clarification agent for highly turbid and untreated pathogenic surface water. Efficient reduction (80.0% to 99.5%) of high turbidity produces an aesthetically clear supernatant, concurrently accompanied by 90.00% to 99.99% (1 to 4 log) bacterial reduction. Application of this low-cost Moringa oleifera protocol is recommended for simplified, point-of-use, low-risk water treatment where rural and peri-urban people living in extreme poverty are presently drinking highly turbid and microbiologically contaminated water. PMID:20131221

Lea, Michael

2010-02-01

331

Triterpenes and Steroids from Euphorbia denticulata Lam. With Anti-Herpes Symplex Virus Activity  

PubMed Central

In this research, dried acetone:chloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3?,25-diol, cycloart-23E-ene-3?,25- diol, ergosta-8,24-dien-3-ol (obtusifoliol) and beta-sitosterol which were reported for the first time from this plant. The structure of these compounds was elucidated by NMR and mass spectroscopic methods. The MTS assay was used to determine the toxicity and antiviral activity of betulin and (3?,23E)-cycloarta-23-ene-3,25-diol. Betulin showed anti-HSV-1 activity with EC50 value of 84.37±0.02 ?g/mL, and toxicity on normal vero cells with CC50 value of 660.718±0.072 ?g/mL. (3?,23E)-Cycloarta-23-ene-3,25-diol showed antiviral effect with EC50 value of 86.63±0.03 ?g/mL, and toxicity with CC50 value of 1089.21±0.25 ?g/mL. The results revealed that these two compounds have the antiviral activity far below the CC50 value with selectivity index (CC50/EC50) values of 7.83, and 12.57, respectively. PMID:24523756

Shamsabadipour, Sara; Ghanadian, Mustafa; Saeedi, Hojjatollah; Rahimnejad, Mohammad Reza; Mohammadi-Kamalabadi, Marzieh; Ayatollahi, Seyed Majid; Salimzadeh, Loghman

2013-01-01

332

UBC Social Ecological Economic Development Studies (SEEDS) Student Report Kevin Petersen, On Tak Lam, Zhejian Zhang  

E-print Network

. With this consumption, there is the opportunity to remanufacture the used cartridges and reduce the carbon footprint figures in regards to the waste and the reduction the carbon footprint are not available due will on reduce the costs of obtaining cartridges from 10%-30% while also reducing the carbon footprint

333

Fruit of the jambolan tree (Eugenia jambolana Lam.) and experimental diabetes.  

PubMed

The fruit of Indian Eugenia jambolana have been shown to have therapeutic properties, but because the therapeutic potential of a plant is related to the geographic region in which the plant was grown and to the part of the plant used, we investigated Brazilian Eugenia jambolana fruit using the same preparation and experimental methods as have been used in India. The well-established metabolic cage model was used to evaluate the physiological and metabolic parameters associated with streptozotocin-induced diabetes in rats (n=10) which had been administered, by gavage, 50 mg per day of lyophilised Eugenia jambolana fruit-pulp extract for 41 days. We found that, compared to untreated controls, rats treated with the lyophilised fruit-pulp showed no observable difference in body weight, food or water intake, urine volume, glycaemia, urinary urea and glucose, hepatic glycogen, or on serum levels of total cholesterol, HDL cholesterol or triglycerides. No change was observed in the masses of epididymal or retroperitoneal adipose tissue or of soleus or extensor digitorum longus muscles. This lack of any apparent effect on the diabetes may be attributable to the regional ecosystem where the fruit was collected and/or to the severity of the induced diabetes. PMID:15588649

Pepato, M T; Mori, D M; Baviera, A M; Harami, J B; Vendramini, R C; Brunetti, I L

2005-01-01

334

Bacterial Source Tracking to Support the Development and Implementation of Watershed Protection Plans for the Lampasas and Leon Rivers: Lampasas River Watershed Final Report  

E-print Network

(mg/L) pH Conductance (?S/cm) Flow (CFS) LAM 1 15762 16.14 6.62 7.37 468 0.14 LAM 2 21013 16.96 9.17 7.25 529 1.96 LAM 3 15770 18.69 8.04 7.64 556 4.17 LAM 4 15763 14.92 8.85 7.48 527 3.24 LAM 5 21014 14.35 8.06 7.10 262 2.30 LAM 6 11872 20... from flowing water in the Lampasas River watershed. Station TCEQ Station ID # of Samples Geometric Mean (CFU/100mL) LAM 1 15762 3 162 LAM 2 21013 7 66 LAM 3 15770 6 158 LAM 4 15763 7 33 LAM 5 21014 2 3257 LAM 6 11872 12 64 LAM 7 15781 12...

Gregory, L.; Casarez, E.; Truesdale, J.; Di Giovanni, G.; Owen, T; Wolfe, J.

2013-04-25

335

Isolation and characterization of microsatellite markers in the invasive shrub Mahonia aquifolium (Berberidaceae) and their applicability in related species  

Microsoft Academic Search

Microsatellite loci were isolated from a Mahonia aquifolium cultivar. We describe the var- iability of 10 loci in invasive European and native North American M. aquifolium and their transspecies amplification in native Mahonia repens and Mahonia pinnata from North America and one species of the related genus Berberis ( Berberis vulgaris ), native to Europe. The markers should be useful

C. ROss; W. DURKA

2006-01-01

336

Molecular analysis of Iranian seedless barberries via SSR  

Microsoft Academic Search

Little is known about the genetic diversity of seedless barberry cultivars and its wild type relatives. Microsatellite markers next to the morphological traits were used to distinguish the relatedness of some 47 genotypes; forty-five Iranian genotypes including both seedless cultivars and wild types and two foreign genotypes: Berberis vulgaris and Berberis thunbergii. SSR primers were designed based on seven Mahonia

Mehdi Rezaei; Ali Ebadi; Stefanie Reim; Reza Fatahi; Ahmad Balandary; Naser Farrokhi; Magda-Viola Hanke

2011-01-01

337

OIKOS 42: 166-170. Copenhagen 1984 Selective pressures on fruit seediness: differential predation of  

E-print Network

OIKOS 42: 166-170. Copenhagen 1984 Selective pressures on fruit seediness: differential predation of fly larvae on the fruits of Berberis hispanica Carlos M. Herrera Herrera, C. M. 1984. Selective pressures on fruit seediness: differential predation of fly larvae on the fruits of Berberis hispanica

Herrera, Carlos M.

338

Effect of cutting type on the rooting process in selected species of evergreen barberries  

Microsoft Academic Search

The experiment was performed on two evergreen barberry species, Berberis julianae (wintergreen barberry) and Berberis verruculosa (warty barberry), both considered to be relati - vely winter-hardy and suitable for growing under the climate conditions of Poland. The objective of the study was to determine the effect of propagation method on rooting percentage and root system quality in the barberry. Stem

Urszula Puczel

339

Self-compatibility in Lolium temulentum L: its genetic control and transfer into L. perenne L. and L. multiflorum Lam  

Microsoft Academic Search

The segregation of self-compatibility from L. temulentum was examined in backcross generations derived from hybrids between this species and the self-incompatible species, L. perenne and L. multiflorum, the latter being used as recurrent parents. Segregation patterns for self-compatibility were determined by percentage self seed set and by in-vitro self-pollination scores in the resulting backcross generations. Self-compatibility in L. temulentum is

D Thorogood; M D Hayward

1992-01-01

340

LAM: A landscape matching algorithm for respiratory data alignment Liangyou Chen, Thomas McKenna, Andrei Gribok, Jaques Reifman*  

E-print Network

,4 to index cardiac parasympathetic control,5 and to estimate trauma severity.6 Moreover, patterns for the development of automated diagnostic and/or prognostic algorithms to assist in emergency care and combat, collected during transport from

341

VizieR Online Data Catalog: lam Ori and sig Ori low-mass stars spectroscopy (Maxted+, 2008)  

NASA Astrophysics Data System (ADS)

Radial velocities and spectral indices for 218 stars and brown dwarfs around {sigma} and {lambda} Orionis. Observations were obtained with the FLAMES/GIRAFFE multi-object spectrograph on ESO's VLT UT2 (Kueyen) telescope (programme ID 076.C_145). The equivalent width of the NaI 8200{AA} and the strength of the TiO 8442{AA} spectral features were measured from the median average spectrum. Radial velocities were measured by cross-correlation against a template spectrum of the brown dwarf star USco CTIO 055 at multiple epochs. (3 data files).

Maxted, P. F. L.; Jeffries, R. D.; Oliveira, J. M.; Naylor, T.; Jackson, R. J.

2008-05-01

342

Effect of heat–moisture treatment on digestibility of different cultivars of sweet potato (Ipomea batatas (L.) Lam) starch  

PubMed Central

Different heat–moisture levels were applied to native starches from different cultivars of sweet potatoes available in Sri Lanka (Wariyapola red, Wariyapola white, Pallepola variety, Malaysian variety and CARI 273) to study the digestibility level. Samples were treated with 20, 25, and 30% moisture at 85°C and 120°C for 6 h and in vitro starch digestibility was tested with porcine pancreatin enzyme. A range of 19.3–23.5% digestibility was shown by the native starches with no significant difference (P < 0.05). Significant changes were observed in the digestibility level of the hydrothermally modified starches and the moisture content showed a positive impact on the digestibility. Heat–moisture treatment at 85°C brought an overall increase in digestibility and temperature beyond 85°C had a negative impact. No significant difference (P < 0.05) in the digestibility was observed with 20% and 25% moisture at 85°C and increased level were seen at 85°C and 30% moisture. PMID:25473497

Senanayake, Suraji; Gunaratne, Anil; Ranaweera, K K D S; Bamunuarachchi, Arthur

2014-01-01

343

Desenvolvimento de metodologia analítica para extração e pré-concentração de Ag(I) utilizando a moringa oleifera Lam.  

E-print Network

??Processos industriais tem sido historicamente um importante fator de degradação ambiental. A disposição inadequada de resíduos industriais contendo metais tóxicos em fontes de águas representa… (more)

Cleide Sandra Tavares Araújo

2009-01-01

344

Contact-Printed Microelectromechanical Systems By Corinne E. Packard, Apoorva Murarka, Eric W. Lam, Martin A. Schmidt,  

E-print Network

, Martin A. Schmidt, and Vladimir Bulovic´* Standard photolithography-based methods for fabricating micro adaptive-texture display applications. The methods and tools used in the mature field of microelectronics fabricated within the existing silicon microelectronics-based framework also inherit the limitations

345

Quy v co the lam theo cac bc sau e cai tien chat lng khong kh trong nha  

E-print Network

neân duøng bình xòt coân truøng. Khi duøng trong khoâng gian chaät heïp nhö nhaø löu ñoäng FEMA, chaát Löôïng Khoâng Khí vaø Söùc Khoûe trong Nhaø Taïm Cö FEMA Cho cö daân nhaø löu ñoäng Trung Taâm Kieåm chaát löôïng khoâng khí vaø formandehit trong nhaø löu ñoäng cuûa FEMA. #12;CDC laøm gì ñeå giuùp quyù

346

Chemical composition and biological activity of the essential oil from leaves of Moringa oleifera Lam. cultivated in Mozambique.  

PubMed

The antioxidant capacity and antimicrobial activity of the essential oil of Moringa oleifera (Moringaceae) grown in Mozambique was investigated. The chemical composition was studied by means of GC and GC-MS analysis. Hexacosane (13.9%), pentacosane (13.3%) and heptacosane (11.4%) were the main components. Ultra High Performance Chromatography-DAD analysis detected the flavonoids quercetin (126 ?g/g) and luteolin (6.2 ?g/g). The essential oil exhibited a relatively low free radical scavenging capacity. The antimicrobial activity of the essential oil was assayed against two Gram-positive strains (Bacillus cereus, Staphylococcus aureus), two Gram-negative strains (Escherichia coli, Pseudomonas aeruginosa), and five fungal strains of agro-food interest (Penicillium aurantiogriseum, Penicillium expansum, Penicillium citrinum, Penicillium digitatum, and Aspergillus niger spp.). B. cereus and P. aeruginosa, as well as the fungal strains were sensitive to the essential oil. PMID:24022760

Marrufo, Tatiana; Nazzaro, Filomena; Mancini, Emilia; Fratianni, Florinda; Coppola, Raffaele; De Martino, Laura; Agostinho, Adelaide Bela; De Feo, Vincenzo

2013-01-01

347

On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity  

PubMed Central

New Guinea is considered the most important secondary centre of diversity for sweet potato (Ipomoea batatas). We analysed nuclear and chloroplast genetic diversity of 417 New Guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical America. The molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical America. Nuclear data confirm previous results, suggesting that New Guinea landraces are principally derived from the Northern neotropical genepool (Camote and Batata lines, from the Caribbean and Central America). However, chloroplast data suggest that South American clones (early Kumara line clones or, more probably, later reintroductions) were also introduced into New Guinea and then recombined with existing genotypes. The frequency distribution of pairwise distances between New Guinea landraces suggests that sexual reproduction, rather than somaclonal variation, has played a predominant role in the diversification of sweet potato. The frequent incorporation of plants issued from true seed by farmers, and the geographical and cultural barriers constraining crop diffusion in this topographically and linguistically heterogeneous island, has led to the accumulation of an impressive number of variants. As the diversification of sweet potato in New Guinea is primarily the result of farmers' management of the reproductive biology of their crop, we argue that on-farm conservation programmes that implement distribution of core samples (clones representing the useful diversity of the species) and promote on-farm selection of locally adapted variants may allow local communities to fashion relatively autonomous strategies for coping with ongoing global change. PMID:23531982

Roullier, C; Kambouo, R; Paofa, J; McKey, D; Lebot, V

2013-01-01

348

Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam.  

PubMed

Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines. PMID:25189525

Rathi Sre, P R; Reka, M; Poovazhagi, R; Arul Kumar, M; Murugesan, K

2015-01-25

349

Anti-acetylcholinesterase and antioxidant activities and HPLC-MS analysis of polyphenol from extracts of Nelsonia canescens (Lam.) Spreng.  

PubMed Central

Objective To investigate the anti-acetylcholinesterase and antioxidant activities and to evaluate the major polyphenolic compounds of Nelsonia canescens extracts. Methods The anti-acetylcholinesterase activity was assessed using a kinetic inhibition standard method. Two methods, ABTS and lipid peroxidation, were used to estimate the antioxidant capacity. Polyphenols profile of the plant extract has been determined with a HPLC-MS method. Results The results showed that butanol extract exhibited the best anti-acetylcholinesterase activity with inhibition percentage of (55.62±1.49)%. The best 3 ethylbenzothiazoline-6-sulphonate radical cation scavenging capacity was found for ethyl acetate extract with a value of (56.20±0.77) mg equivalent trolox/g while the crude extract showed the highest inhibition of the rat liver lipid peroxidation (52.57±1.20)%. Polyphenols profile revealed the presence of five phenol acids (p-coumaric acid, caffeic acid, chlorogenic acid, ferulic acid and gentisic acid) and three flavonoids (apigenin, luteolin, quercetin). Conclusions All the extracts of Nelsonia canescens exhibited antioxidant and AChE inhibition capacities. The active compounds identified and quantified in this species are mainly responsible for these in vitro biological activities and allow to justify its widely use in Burkina Faso traditional medicine.

Ouattara, Nabèrè; Meda, Roland Nâg-Tiero; Hilou, Adama; Guenné, Samson; Konaté, Kiessoum; Coulibaly, Ahmed Y; Kiendrébeogo, Martin; Millogo, Jeanne F; Nacoulma, Odile G

2013-01-01

350

Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam  

NASA Astrophysics Data System (ADS)

Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines.

Rathi Sre, P. R.; Reka, M.; Poovazhagi, R.; Arul Kumar, M.; Murugesan, K.

2015-01-01

351

Juice of Bryophyllum pinnatum (Lam.) inhibits oxytocin-induced increase of the intracellular calcium concentration in human myometrial cells.  

PubMed

The use of preparations from Bryophyllum pinnatum in tocolysis is supported by both clinical (retrospective comparative studies) and experimental (using uterus strips) evidence. We studied here the effect of B. pinnatum juice on the response of cultured human myometrial cells to stimulation by oxytocin, a hormone known to be involved in the control of uterine contractions by increasing the intracellular free calcium concentration ([Ca2+]i). In this work, [Ca2+]i was measured online during stimulation of human myometrial cells (hTERT-C3 and M11) with oxytocin, which had been pre-incubated in the absence or in the presence of B. pinnatum juice. Since no functional voltage-gated Ca2+ channels could be detected in these myometrial cells, the effect of B. pinnatum juice was as well studied in SH-SY5Y neuroblastoma cells, which are known to have such channels and can be depolarised with KCl. B. pinnatum juice prevented the oxytocin-induced increase in [Ca2+]i in hTERT-C3 human myometrial cells in a dose-dependent manner, achieving a ca. 80% inhibition at a 2% concentration. Comparable results were obtained with M11 human primary myometrial cells. In hTERT-C3 cells, prevention of the oxytocin-induced increase in [Ca2+]i was independent of the extracellular Ca2+ concentration and of voltage-dependent Ca2+-channels. B. pinnatum juice delayed, but did not prevent the depolarization-induced increase in [Ca2+]i in SH-SY5Y cells. Taken together, the data suggest a specific and concentration-dependent effect of B. pinnatum juice on the oxytocin signalling pathway, which seems to corroborate its use in tocolysis. Such a specific mechanism would explain the rare and minor side-effects in tocolysis with B. pinnatum as well as its high therapeutic index. PMID:20381326

Simões-Wüst, A P; Grãos, M; Duarte, C B; Brenneisen, R; Hamburger, M; Mennet, M; Ramos, M H; Schnelle, M; Wächter, R; Worel, A M; von Mandach, U

2010-10-01

352

The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves  

Microsoft Academic Search

Moringa oleifera is used in Thai traditional medicine as cardiotonic. Recent studies demonstrated its hypocholesterolaemic effect. However, to be clinically useful, more scientific data are needed. Aim of the Study: We investigated the antioxidant, hypolipidaemic and antiatherosclerotic activities of Moringa oleifera leaf extract. Materials and Methods: Scavenging activity of the extract on 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), and the inhibitory effect on

Pilaipark Chumark; Panya Khunawat; Yupin Sanvarinda; Srichan Phornchirasilp; Noppawan Phumala Morales; Laddawal Phivthong-ngam; Piyanee Ratanachamnong; Supath Srisawat; Klai-upsorn S. Pongrapeeporn

2008-01-01

353

Evaluation of seasonal chemical composition, antibacterial, antioxidant and anticholinesterase activity of essential oil from Eugenia brasiliensis Lam.  

PubMed

This study describes the seasonal composition and the antibacterial, antioxidant and anticholinesterase activity of the essential oil from Eugenia brasiliensis leaves. Analysis by using GC allowed the identification of 40 compounds. It was observed that the monoterpenes varied more (42%) than the sesquiterpenes (14%), and that the monoterpene hydrocarbons suffered the greatest variation throughout the year (64%). Major compounds were spathulenol in the spring (16.02 ± 0.44%) and summer (18.17 ± 0.41%), ?-cadinol in the autumn (12.83 ± 0.03%) and ?-pinene (15.94 ± 0.58%) in the winter. Essential oils were tested for their antibacterial activity, and the best result was obtained from the autumn oil, with MIC = 500 ?g mL(- 1) against Staphylococcus saprophyticus and Pseudomonas aeruginosa. Antioxidant activity was evaluated using DPPH, lipid peroxidation and iron-reducing power assays, as well as the anticholinesterase activity. Both tests showed a weak performance of the essential oils. PMID:25219800

Siebert, Diogo Alexandre; Tenfen, Adrielli; Yamanaka, Celina Noriko; de Cordova, Caio Maurício Mendes; Scharf, Dilamara Riva; Simionatto, Edésio Luiz; Alberton, Michele Debiasi

2015-02-01

354

Effect of heat-moisture treatment on digestibility of different cultivars of sweet potato (Ipomea batatas (L.) Lam) starch.  

PubMed

Different heat-moisture levels were applied to native starches from different cultivars of sweet potatoes available in Sri Lanka (Wariyapola red, Wariyapola white, Pallepola variety, Malaysian variety and CARI 273) to study the digestibility level. Samples were treated with 20, 25, and 30% moisture at 85°C and 120°C for 6 h and in vitro starch digestibility was tested with porcine pancreatin enzyme. A range of 19.3-23.5% digestibility was shown by the native starches with no significant difference (P < 0.05). Significant changes were observed in the digestibility level of the hydrothermally modified starches and the moisture content showed a positive impact on the digestibility. Heat-moisture treatment at 85°C brought an overall increase in digestibility and temperature beyond 85°C had a negative impact. No significant difference (P < 0.05) in the digestibility was observed with 20% and 25% moisture at 85°C and increased level were seen at 85°C and 30% moisture. PMID:25473497

Senanayake, Suraji; Gunaratne, Anil; Ranaweera, K K D S; Bamunuarachchi, Arthur

2014-07-01

355

Effects of baking and boiling on the nutritional and antioxidant properties of sweet potato [Ipomoea batatas (L.) Lam.] cultivars.  

PubMed

The effects of baking and boiling on the nutritional and antioxidant properties of three sweet potato cultivars (Beniazuma, Koganesengan, Kotobuki) cultivated in Turkey were investigated. The samples were analyzed for proximate composition, total phenolic content, ascorbic acid, ?-carotene, antiradical activity, and free sugars. The dry matter, protein, and starch contents of the sweet potatoes were significantly changed by the treatments while the ash and crude fiber contents did not differ as significantly. The ?-carotene contents of baked and boiled sweet potatoes were lower than those of fresh sweet potatoes; however, the total phenolic and ascorbic acid contents of the baked and boiled sweet potatoes were higher than those of the fresh samples. Generally, the antiradical activity of the sweet potatoes increased with the treatments. Sucrose, glucose, and fructose were quantified as free sugars in all fresh sweet potatoes; however, maltose was determined in the treated samples. In terms of the analyzed parameters, there were no explicit differences among the sweet potato cultivars. PMID:22101780

Dincer, Cuneyt; Karaoglan, Mert; Erden, Fidan; Tetik, Nedim; Topuz, Ayhan; Ozdemir, Feramuz

2011-11-01

356

Direct effects of Moringa oleifera Lam (Moringaceae) acetone leaf extract on broiler chickens naturally infected with Eimeria species.  

PubMed

Avian coccidiosis is one of the most important diseases of poultry and it is responsible for a large number of all broiler mortalities worldwide. The control of this disease relies mainly on the use of anticoccidial drugs. However, herbal preparations could be an alternative for the treatment against coccidiosis in chickens. The direct effects of Moringa oleifera acetone extracts on broiler chickens naturally infected with mixed Eimeria species was investigated to determine the relative efficacy of the extracts against coccidiosis in birds. The investigations were carried out in seven groups (ten chickens per group). The birds were given various doses (1.0, 2.0, 3.0, 4.0 and 5.0 g/kg body weight) of acetone extract of leaves of M. toltrazuril (positive control) and untreated (negative control). The extract was evaluated for anticoccidial activity by means of inhibition of oocyst output in faeces, faecal score, weight gain and mortality. Haematological indices were evaluated by standard methods. The group treated with 1.0 g/ kg body weight Moringa oleifera extract produced the least inhibitory effect on oocyst shed in the faeces (96.4%), while the groups treated with 2.0 g/kg, 3.0 g/kg, 4.0 g/kg and 5.0 g/kg body weight of the extract produced 97.4, 98.7, 99.1 and 99.8%, respectively. Body weight gains of infected chickens treated with the extract significantly improved (p < 0.05), and faecal scores were milder. Packed cell volume, haemoglobin concentration and red blood count of the treated birds were significantly (p < 0.05) higher than those of the infected untreated group. Moringa oleifera leaves could find application in the treatment of avian coccidiosis in veterinary practice. PMID:23440591

Ola-Fadunsin, Shola David; Ademola, Isaiah Oluwafemi

2013-08-01

357

Exchange-Correlation Energy Density from Virial Theorem Federico G. Cruz, Kin-Chung Lam, and Kieron Burke*  

E-print Network

be approximated as a functional of the spin densities. The local spin density (LSD) approximation Exc LSD has long of exact exchange with GGA exchange-correlation. Typical bond energy errors are about 30 kcal/mol in LSD, 8, some have spurious extrema, and overall, look worse than their LSD counterparts. So how do they produce

Burke, Kieron

358

Anatomy and ultrastructure alterations of Leucaena leucocephala (Lam.) inoculated with mycorrhizal fungi in response to arsenic-contaminated soil.  

PubMed

Many studies demonstrate the potential application of arbuscular mycorrhizal fungi (AMF) for remediation purposes, but little is known on AMF potential to enhance plant tolerance to arsenic (As) and the mechanisms involved in this process. We carried anatomical and ultrastructural studies to examine this symbiotic association and the characteristics of shoots and roots of Leucaena leucocephala in As-amended soils (35 and 75 mg As dm(-3)). The experiment used 3 AMF isolates from uncontaminated soils: Acaulospora morrowiae, Glomus clarum, and Gigaspora albida; a mixed inoculum derived from combining these 3 isolates (named Mix AMF); and, 3 AMF isolates from As-contaminated areas: A. morrowiae, G. clarum and Paraglomus occultum. Phytotoxicity symptoms due to arsenic contamination appeared during plant growth, especially in treatments without AMF application. Inoculation with G. clarum and the mixture of species (A. morrowiae, G. albida, and G. clarum) resulted in better growth of L. leucocephala in soils with high As concentrations, as well as significant As removal from the soil, showing a potential for using AMF in phytoextraction. Light microscopy (LS), transmission (TEM) and scanning electron microscopies (SEM) studies showed the colonization of the AMF in plant tissues and damage in all treatments, with ultrastructural changes being observed in leaves and roots of L. leucocephala, especially with the addition of 75 mg dm(-3) of As. PMID:22704769

Schneider, Jerusa; Labory, Claudia Regina Gontijo; Rangel, Wesley Melo; Alves, Eduardo; Guilherme, Luiz Roberto Guimarães

2013-11-15

359

Evaluation of a cost effective technique for treating aquaculture water discharge using Lolium perenne Lam as a biofilter  

Microsoft Academic Search

Wastewater stabilization ponds generate low cost by-products that are useful for agriculture. The utilization of these by-products for soil amendment and as a source of nutrients for plants requires a high level of sanitation and stabilization of the organic matter, to maintain acceptable levels of soil, water and air quality. In this study, two aquaculture wastewater treatment systems; recirculating system

André NDUWIMANA; Xiang-long YANG; Li-ren WANG

2007-01-01

360

From introduced American weed to Cape Verde Islands endemic: the case of Solanum rigidum Lam. (Solanaceae, Solanum subgenus Leptostemonum)  

PubMed Central

Abstract A Solanum species long considered an American introduction to the Cape Verde Islands off the west coast of Africa is identified as Solanum rigidum, a member of the Eggplant clade of Old World spiny solanums (Solanum subgenus Leptostemonum) and is probably endemic to the Cape Verde Islands. Collections of this species from the Caribbean are likely to have been introduced from the Cape Verde Islands on slave ships. We discuss the complex nomenclatural history of this plant and provide a detailed description, illustration and distribution map. The preliminary conservation status of Solanum rigidum is Least Concern, but needs to be reassessed in light of its endemic rather than introduced status. PMID:24198710

Knapp, Sandra; Vorontsova, Maria S.

2013-01-01

361

The Hf isotope composition of cratonic mantle: LAM-MC-ICPMS analysis of zircon megacrysts in kimberlites  

Microsoft Academic Search

Zircon megacrysts represent a late stage in the crystallisation of the magmas that produced the low-Cr megacryst suite (Ol+Opx+Cpx+Gnt+Ilm+Phl+Zir) found in many kimberlites, and may carry information on the sources of the parent magmas and the interaction of these magmas with the cratonic lithosphere. The isotopic composition of Hf has been measured in 124 mantle-derived zircon megacrysts from African, Siberian

W. L. Griffin; N. J. Pearson; E. Belousova; S. E. Jackson; E. van Achterbergh; Suzanne Y. O’Reilly; S. R. Shee

2000-01-01

362

Hilbert phase analysis of the dynamics of a semiconductor laser with optical feedback Wing-Shun Lam,1,2  

E-print Network

the interplay of high-dimensional dynamics and noise can be studied. Depending on the amount of feedback threshold is sub- ject to moderate feedback from a distant mirror, the output power drops to almost zero. In the experiment, a temperature controller is used to sta- bilize to better than 0.01 K a Fabry-Perot semiconductor

Rubloff, Gary W.

363

Bolboschoenus glaucus (Lam.) S.G. Smith, a new species in the flora of the ancient Near East  

Microsoft Academic Search

Taxonomic advancement in the genus Bolboschoenus (Cyperaceae, formerly included in the genus Scirpus) have resulted in the re-classification of the plant previously known as Bolboschoenus maritimus (synonym Scirpus maritimus) into several closely-related but distinct Bolboschoenus species This improved taxonomy is of importance for archaeobotanical investigations of ancient sites within the temperate\\u000a zones, where this genus frequently occurs, because it allows

Michèle M. Wollstonecroft; Zdenka Hroudová; Gordon C. Hillman; Dorian Q. Fuller

364

Constituents of Cajanus cajan (L.) Millsp., Moringa oleifera Lam., Heliotropium indicum L. and Bidens pilosa L. from Nigeria.  

PubMed

The essential oils of four plant species from Nigeria have been extracted by hydrodistillation and analyzed by GC and GC-MS. The oils of Cajanus cajan were comprised of sesquiterpenes (92.5%, 81.2% and 94.3% respectively in the leaves, stem and seeds). The major compounds identified were alpha-himachalene (9.0-11.5%), beta-himachalene (8.0-11.0%), gamma-himachalene (6.9-8.1%), alpha-humulene (7.1-8.7%) and alpha-copaene (4.5-5.6%). However, monoterpenoid compounds (81.8%) dominated the oil of Moringa oleifera with an abundance of alpha-phellandrene (25.2%) and p-cymene (24.9%). On the other hand, aldehydes (52.8%) occurred in the highest amount in Heliotropium indicum, represented by phenylacetaldehyde (22.2%), (E)-2-nonenal (8.3%) and (E, Z)-2-nonadienal (6.1%), with a significant quantity of hexahydrofarnesylacetone (8.4%). The leaf and stem oils of Bidens pilosa were dominated by sesquiterpenes (82.3% and 59.3%, respectively). The main compounds in the leaf oil were caryophyllene oxide (37.0%), beta-caryophyllene (10.5%) and humulene oxide (6.0%), while the stem oils had an abundance of hexahydrofarnesyl acetone (13.4%), delta-cadinene (12.0%) and caryophyllene oxide (11.0%). The observed chemical patterns differ considerably from previous investigations. PMID:19476009

Ogunbinu, Akinola O; Flamini, Guido; Cioni, Pier L; Adebayo, Muritala A; Ogunwande, Isiaka A

2009-04-01

365

Antiviral activity of crude extracts of Eugenia jambolana Lam. against highly pathogenic avian influenza (H5N1) virus.  

PubMed

Crude extracts of leaves and bark of E. jambolana were tested for antiviral activity against highly pathogenic avian influenza virus (H5N1) by CPE reduction assay in three different layouts to elucidate virucidal, post-exposure and preexposure antiviral activity of the extracts. The cold and hot aqueous extracts of bark and hot aqueous extract of leaves of E. jambolana showed significant virucidal activity (100% inhibition) which was further confirmed in virus yield reduction assay (-98 to 99% reduction) and by egg based in ovo assay. The selective index (CC50/EC50) of hot aqueous extract (248) and cold aqueous extract (43.5) of bark of E. jambolana showed their antiviral potential against H5N1 virus. The significant virucidal activity of leaves and bark of E. jambolana merits further investigation as it may provide alternative antiviral agent for managing avian influenza infections in poultry farms and potential avian-human transmission. PMID:22439432

Sood, Richa; Swarup, D; Bhatia, S; Kulkarni, D D; Dey, S; Saini, M; Dubey, S C

2012-03-01

366

Proceedings of the 17th Central Hardwood Forest Conference GTR-NRS-P-78 (2011) 650 Japanese barberry (Berberis thunbergii) is classified as invasive in 20 states and four Canadian provinces. It is  

E-print Network

the following summer will be needed for eradication. Control of Japanese barberry had an effect on earthworm biomass (F = 6.86, d.f. = 2, P Earthworm biomass averaged 197 pounds per acre where barberry was controlled and 535 pounds per acre in dense barberry infestations. By comparison, earthworm biomass was 304

367

Berberine.  

PubMed

Berberine is a plant alkaloid with a long history of medicinal use in both Ayurvedic and Chinese medicine. It is present in Hydrastis canadensis (goldenseal), Coptis chinensis (Coptis or goldenthread), Berberis aquifolium (Oregon grape), Berberis vulgaris (barberry), and Berberis aristata (tree turmeric). The berberine alkaloid can be found in the roots, rhizomes, and stem bark of the plants. Berberine extracts and decoctions have demonstrated significant antimicrobial activity against a variety of organisms including bacteria, viruses, fungi, protozoans, helminths, and chlamydia. Currently, the predominant clinical uses of berberine include bacterial diarrhea, intestinal parasite infections, and ocular trachoma infections. PMID:10767672

2000-04-01

368

Special Equipment Recharge Rates As of Dec 10, 2014, 5:00 AM  

E-print Network

Special Equipment Recharge Rates As of Dec 10, 2014, 5:00 AM Name Description Price Unit aln2 Tegal.70 minute lam7 Lam Research 9626 Aluminum Etch $0.70 minute lam8 9426 Lam Research TCP MOS Poly $0.70 minute:00 AM Name Description Price Unit oxford Oxford Plasmalab System 100 $0.70 minute oxford-icp Oxford

Healy, Kevin Edward

369

7 CFR 301.38-1 - Definitions.  

Code of Federal Regulations, 2010 CFR

...Administrator. Animal and Plant Health Inspection Service...APHIS). The Animal and Plant Health Inspection Service...asexually through cuttings, tissue culture, suckers, or crown...subpart, a Berberis plant will be considered...

2010-01-01

370

Alternative Species Taxonomic Name: Cornus mas  

E-print Network

and provide color in the landscape when it is typically lacking. #12;Fruit: Cornus mas, again like Berberis, has red oval fruit. Bark: The bark is exfoliating and produces mottled patches of tans and browns

Hayden, Nancy J.

371

Regulation of leukocyte adhesion molecule-1 (TQ1, Leu-8) expression and shedding by normal and malignant cells.  

PubMed

The human leukocyte adhesion molecule-1 (LAM-1, TQ1, Leu-8) is involved in the binding of human leukocytes to high endothelial venules (HEV) of peripheral lymph nodes (LN). The regulation of LAM-1 expression is unique in that leukocyte stimulation induces a rapid down-modulation of LAM-1 from the cell surface. In this study, the regulation and function of LAM-1 was studied in detail in normal lymphocytes and compared with the LAM-1 of malignant leukocytes. Modulation of LAM-1 from the cell surface occurred concomitantly with the appearance of LAM-1 in the culture medium indicating that LAM-1 is cleaved from the cell surface. Shedding of LAM-1 was decreased in the presence of protein kinase C (PKC) inhibitors. As with normal lymphocytes, cells transfected with the LAM-1 cDNA and chronic lymphocytic leukemia (CLL) cells also shed LAM-1 following phorbol myristate acetate (PMA) exposure. CLL cells expressed the same Mr LAM-1 protein as normal lymphocytes and LAM-1+ CLL cells were able to specifically bind to HEV. In addition, normal lymphocytes and LAM-1+ CLL cells were capable of binding polyphosphomonester core polysaccharide (PPME) derived from yeast cell wall, a carbohydrate which mimics an essential component of the natural ligand for LAM-1, and PPME and HEV binding was specifically blocked by a new monoclonal antibody (mAb) reactive with LAM-1. The expression of LAM-1 and other adhesion molecules was examined on cells of 118 hematopoietic malignancies. LAM-1 was most frequently expressed on CLL and follicular or diffuse small cleaved cell lymphomas, whereas most other malignancies were LAM-1-. Thus, most CLL cells and some non-Hodgkin's lymphoma cells express a functionally active LAM-1 molecule which may correlate with their capacity to migrate through the circulation and disseminate into peripheral LN. PMID:1709244

Spertini, O; Freedman, A S; Belvin, M P; Penta, A C; Griffin, J D; Tedder, T F

1991-04-01

372

Composition and allelopathic effect of essential oils of two thistles: Cirsium creticum (Lam.) D.'Urv. ssp. triumfetti (Lacaita) Werner and Carduus nutans L  

Microsoft Academic Search

Cirsium and Carduus are two of the main genera of true thistles. Different species in these genera share a quantity of secondary metabolites and have interesting ecological properties. The essential oils of two species, Cirsium creticum and Carduus nutans, were analysed, showing the presence as main compounds of 4-ethyl guaiacol (15%), hexadecanoic acid (10.6%), (E)-?-damascenone (7.8%), dihydroactinidiolide (6.0%) and 4-vinyl

Carmen Formisano; Daniela Rigano; Felice Senatore; Vincenzo De Feo; Maurizio Bruno; Sergio Rosselli

2007-01-01

373

Abstract --This paper presents an approach for modeling and model-checking a mobile agent system specified by LAM, which is a two-layer  

E-print Network

Predicate/Transition (PrT) nets. Based on the transformation of PrT nets into input programs of the model], to formally modeling logical agent mobility based on Predicate/Transition (PrT) nets. The semantics of a two USA (e-mail: {hex, deng}@cs.fiu.edu). defined by transforming it into a complete PrT net. System

374

A Nonhost Peptidase Inhibitor of ~14?kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera  

PubMed Central

Helicoverpa armigera is one of the major devastating pests of crop plants. In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H. armigera larvae. B. monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14?kDa with or without reduction. In vitro studies towards total gut proteolytic enzymes of H. armigera and bovine trypsin indicated measurable inhibitory activity. B. monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively. The IC50 of B. monosperma peptidase inhibitor against total H. armigera gut proteinases activity was 2.0?µg/mL. The larval feeding assays suggested B. monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H. armigera. Supplementing B. monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced. The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H. armigera suggest its usefulness in insect pest management of food crops. PMID:24860667

Pandey, Prabhash K.; Singh, Dushyant; Singh, Sangram; Khan, M. Y.; Jamal, Farrukh

2014-01-01

375

Evaluation of nutritional quality of moringa ( Moringa oleifera Lam.) leaves as an alternative protein source for Nile tilapia ( Oreochromis niloticus L.)  

Microsoft Academic Search

This study was conducted to evaluate the suitability of freeze-dried moringa leaf meal, Moringa oleifera, as an alternative protein source for Nile tilapia. Three experimental diets were formulated to contain moringa leaf meal at levels of 10%, 20% and 30% of the total dietary protein (Diets 2, 3 and 4, respectively) and one diet acting as a control (Diet 1)

Nahid Richter; Perumal Siddhuraju; Klaus Becker

2003-01-01

376

Atividades melanogÃnica, genotÃxica e antiproliferativa de extratos de Brosimum gaudichaudii TrÃcul e Dorstenia brasiliensis Lam induzidas por radiaÃÃo UVA.  

E-print Network

??As furanocumarinas lineares ou psoralenos sÃo fotossensibilizadores empregados na dermatologia associados a ultravioleta A no tratamento de doenÃas como o vitiligo. A fotoquimioterapia tem reaÃÃes… (more)

Adriana Elias Pires Quevedo

2011-01-01

377

The Antidiabetic Effect of Low Doses of Moringa oleifera Lam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats.  

PubMed

The antidiabetic activity of two low doses of Moringa seed powder (50 and 100?mg/kg body weight, in the diet) on streptozotocin (STZ) induced diabetes male rats was investigated. Forty rats were divided into four groups. The diabetic positive control (STZ treated) group showed increased lipid peroxide, increased IL-6, and decreased antioxidant enzyme in the serum and kidney tissue homogenate compared with that of the negative control group. Immunoglobulins (IgA, IgG), fasting blood sugar, and glycosylated hemoglobin (HbA1c) were also increased as a result of diabetes in G2 rats. Moreover albumin was decreased, and liver enzymes and ?-amylase were not affected. In addition, the renal functions and potassium and sodium levels in G2 were increased as a sign of diabetic nephropathy. Urine analysis showed also glucosuria and increased potassium, sodium, creatinine, uric acid, and albumin levels. Kidney and pancreas tissues showed also pathological alteration compared to the negative control group. Treating the diabetic rats with 50 or 100?mg Moringa seeds powder/kg body weight in G3 and G4, respectively, ameliorated the levels of all these parameters approaching the negative control values and restored the normal histology of both kidney and pancreas compared with that of the diabetic positive control group. PMID:25629046

Al-Malki, Abdulrahman L; El Rabey, Haddad A

2015-01-01

378

Facile Synthesis of a Tungsten Alkylidyne Catalyst for Alkyne Zachary J. Tonzetich, Yan Choi Lam, Peter Muller, and Richard R. Schrock*  

E-print Network

Facile Synthesis of a Tungsten Alkylidyne Catalyst for Alkyne Metathesis Zachary J. Tonzetich, Yan cis double bonds. Tungsten alkylidyne trialkoxide alkyne metathesis catalysts were discovered in 1981 of cleavage of a tungsten-tungsten triple bond upon reaction with an alkyne or nitrile.9 Recent advances

Müller, Peter

379

Chemical composition and general toxicity of essential oils extracted from the aerial parts of Artemisia armeniaca Lam. and A. incana (L.) Druce growing in Iran  

PubMed Central

The essential oils of the aerial parts of A. armeniaca and A. incana, collected from Arasbaran area (East Azarbaijan province, Iran) were extracted by hydrodistillation and analyzed by GC-MS. In total, 16 and 40 constituents were identified and quantified in the oils of A. armeniaca and A. incana representing 80.5% and 84.6% of the oils, respectively. The essential oil of A. armeniaca was mainly composed of non-terpene hydrocarbons (24.8%). The major components of the oil were ?–pinene (10.7%), nonadecane (10.0%), 6,10,14-trimethyl-2-pentadecanone (9.4%), spathulenol (7.8%) and Z-verbenol (5.8%). The essential oil of A. incana was dominated by oxygenated monoterpenes (41.6%), with camphor (20.4%), 1,8-cineol (10.3%), Z-verbenol (8.7%), ?-thujone (8.3%) and ?-thujone (5.6%), as major components. The essential oils were also subjected to general toxicity assay using brine shrimp lethality method. The toxicity profile of both oils indicated some degree of toxicity in comparison with podophyllotoxin. PMID:24459478

Mojarrab, M.; Delazar, A.; Esnaashari, S.; Afshar, F. Heshmati

2013-01-01

380

A Secure Image Transfer Application for the NHSnet Kerry Jean kjean@ee.ucl.ac.uk, Walter Eaves weaves@ee.ucl.ac.uk, John Lam  

E-print Network

A Secure Image Transfer Application for the NHSnet Kerry Jean kjean@ee.ucl.ac.uk, Walter Eaves of Electronic Engineering, University College London Abstract: This paper outlines the application of some of the security technologies investigated in the HARP (Harmonisation for the security of web technologies and a

Haddadi, Hamed

381

The Antidiabetic Effect of Low Doses of Moringa oleifera Lam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats  

PubMed Central

The antidiabetic activity of two low doses of Moringa seed powder (50 and 100?mg/kg body weight, in the diet) on streptozotocin (STZ) induced diabetes male rats was investigated. Forty rats were divided into four groups. The diabetic positive control (STZ treated) group showed increased lipid peroxide, increased IL-6, and decreased antioxidant enzyme in the serum and kidney tissue homogenate compared with that of the negative control group. Immunoglobulins (IgA, IgG), fasting blood sugar, and glycosylated hemoglobin (HbA1c) were also increased as a result of diabetes in G2 rats. Moreover albumin was decreased, and liver enzymes and ?-amylase were not affected. In addition, the renal functions and potassium and sodium levels in G2 were increased as a sign of diabetic nephropathy. Urine analysis showed also glucosuria and increased potassium, sodium, creatinine, uric acid, and albumin levels. Kidney and pancreas tissues showed also pathological alteration compared to the negative control group. Treating the diabetic rats with 50 or 100?mg Moringa seeds powder/kg body weight in G3 and G4, respectively, ameliorated the levels of all these parameters approaching the negative control values and restored the normal histology of both kidney and pancreas compared with that of the diabetic positive control group.

Al-Malki, Abdulrahman L.; El Rabey, Haddad A.

2015-01-01

382

Chemical composition and general toxicity of essential oils extracted from the aerial parts of Artemisia armeniaca Lam. and A. incana (L.) Druce growing in Iran.  

PubMed

The essential oils of the aerial parts of A. armeniaca and A. incana, collected from Arasbaran area (East Azarbaijan province, Iran) were extracted by hydrodistillation and analyzed by GC-MS. In total, 16 and 40 constituents were identified and quantified in the oils of A. armeniaca and A. incana representing 80.5% and 84.6% of the oils, respectively. The essential oil of A. armeniaca was mainly composed of non-terpene hydrocarbons (24.8%). The major components of the oil were ?-pinene (10.7%), nonadecane (10.0%), 6,10,14-trimethyl-2-pentadecanone (9.4%), spathulenol (7.8%) and Z-verbenol (5.8%). The essential oil of A. incana was dominated by oxygenated monoterpenes (41.6%), with camphor (20.4%), 1,8-cineol (10.3%), Z-verbenol (8.7%), ?-thujone (8.3%) and ?-thujone (5.6%), as major components. The essential oils were also subjected to general toxicity assay using brine shrimp lethality method. The toxicity profile of both oils indicated some degree of toxicity in comparison with podophyllotoxin. PMID:24459478

Mojarrab, M; Delazar, A; Esnaashari, S; Afshar, F Heshmati

2013-01-01

383

Perera Lam: An environmental justice assessment of the Mississippi River Industrial Corridor in Louisiana, U.S. APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 11(4): 681-697.  

E-print Network

report concluded that minority African American communities make up greater proportion of the population in Louisiana. Comparative Environmental Risk Indices (CERI) for poverty and minority computed for parishes Minority Ratios (SMIR) and Standard Poverty Ratios (SPR) provided stronger evidences for environmental

384

Using the Internet in Middle Schools: A Model for Success. A Collaborative Effort between Los Alamos National Laboratory (LANL) and Los Alamos Middle School (LAMS).  

ERIC Educational Resources Information Center

Los Alamos National Laboratory (LANL) developed a model for school networking using Los Alamos Middle School as a testbed. The project was a collaborative effort between the school and the laboratory. The school secured administrative funding for hardware and software; and LANL provided the network architecture, installation, consulting, and…

Addessio, Barbara K.; And Others

385

Factors regulating the growth rate of Lolium perenne L. cv. ‘Grasslands Ruanui’ and L. multiflorum Lam. cv. ‘Grasslands Tama’ a Tetraploid  

Microsoft Academic Search

When seedlings and young plants of these ryegrass varieties are glasshouse-grown in pots with adequate nutrient and light, Tama maintains a 14–16% higher mean relative growth rate (shoot) than Ruanui. A number of developmental, physiological, and biochemical assessments have been made in an attempt to explain some of the reasons for this difference in growth rate. Some discussion on the

A. O. Taylor; J. A. Rowley; N. M. Jepsen

1971-01-01

386

Effect of harvesting interval and defoliation on yield and chemical composition of leaves, stems and tubers of sweet potato ( Ipomoea batatas L. (Lam.)) plant parts  

Microsoft Academic Search

Fifteen sweet potato varieties were grown for 120 days, in two planting seasons in Hue, Vietnam. Leaf, stem and tuber dry matter (DM) yields were measured and chemically analysed. In Experiments 1 and 2, sweet potato vines were harvested at intervals of 15, 20 or 30 days. In Experiment 3, 25, 50, 75 or 100% of the total number of

Le Van An; Bodil E Frankow-Lindberg; Jan Erik Lindberg

2003-01-01

387

Arbuscular mycorrhizal fungi associated with Artemisia umbelliformis Lam, an endangered aromatic species in Southern French Alps, influence plant P and essential oil contents.  

PubMed

Root colonization by arbuscular mycorrhizal (AM) fungi of Artemisia umbelliformis, investigated in natural and cultivated sites in the Southern Alps of France, showed typical structures (arbuscules, vesicles, hyphae) as well as spores and mycelia in its rhizosphere. Several native AM fungi belonging to different Glomeromycota genera were identified as colonizers of A. umbelliformis roots, including Glomus tenue, Glomus intraradices, G. claroideum/etunicatum and a new Acaulospora species. The use of the highly mycorrhizal species Trifolium pratense as a companion plant impacted positively on mycorrhizal colonization of A. umbelliformis under greenhouse conditions. The symbiotic performance of an alpine microbial community including native AM fungi used as inoculum on A. umbelliformis was evaluated in greenhouse conditions by comparison with mycorrhizal responses of two other alpine Artemisia species, Artemisia glacialis and Artemisia genipi Weber. Contrary to A. genipi Weber, both A. umbelliformis and A. glacialis showed a significant increase of P concentration in shoots. Volatile components were analyzed by GC-MS in shoots of A. umbelliformis 6 months after inoculation. The alpine microbial inoculum increased significantly the percentage of E-?-ocimene and reduced those of E-2-decenal and (E,E)-2-4-decadienal indicating an influence of alpine microbial inoculum on essential oil production. This work provides practical indications for the use of native AM fungi for A. umbelliformis field culture. PMID:21243378

Binet, Marie-Noëlle; van Tuinen, Diederik; Deprêtre, Nicolas; Koszela, Nathalie; Chambon, Catherine; Gianinazzi, Silvio

2011-08-01

388

Herbicidal activity of Brassicaceae seed meal on wild oat (Avena fatua), Italian ryegrass (Lolium multiflorum Lam.), redroot pigweed (Amaranthus retroflexus L.) and prickly lettuce (Lactuca serriola)  

Technology Transfer Automated Retrieval System (TEKTRAN)

There is an on-going need for the development of sustainable methods of weed control in crop production systems. Studies were conducted to evaluate the efficacy of different Brassicaceae seed meals and application rates on the emergence of several weed species including wild oat, Italian rye grass, ...

389

Microbial growth and nitrogen immobilization in the root zone of barley ( Hordeum vulgare L.), Italian ryegrass ( Lolium multiflorum Lam.), and white clover ( Trifolium repens L.)  

Microsoft Academic Search

The effect of roots on microbial growth and N immobilization was investigated in a pot experiment with barley, Italian ryegrass, and white clover. We used a silty subsoil with a low soil organic matter content (0.16%C and 0.012%N), which allowed us to measure N immobilization as an increase in total soil organic N (planted versus unplanted). At sampling, the soil

T. A. Breland; L. R. Bakken

1991-01-01

390

The Effects of Drought Stress and Leaf Ageing on Leaf Photosynthesis and Electron Transport in Photosystem 2 in Sweet Potato ( Ipomoea batatas Lam.) Cultivars  

Microsoft Academic Search

Of the four tested sweet potato cultivars having different features in growth and yield, cv. Koganesengan (KOG) was sustainable\\u000a in photosynthetic activity through young to aged leaves under drought. One of the causes for this phenomenon may be stomatal\\u000a conductance (g\\u000a s) of this cultivar that was relatively high in both aged and drought-imposed leaves. In these leaves the non-photochemical

Haimeirong; F. Kubota

2003-01-01

391

A comparison of shoot-forming and non-shoot-forming somatic embryos of sweet potato [Ipomoea batatas (L.) Lam.] using computer vision and histological analyses  

Microsoft Academic Search

Diagnostic structural features for competence to form shoots were tested among sweet potato embryos by combining morphological\\u000a image capture (using a computer vision system) with anatomical analyses (using light microscopy). Five major morphological\\u000a variants (`perfect', `near perfect', `limited\\/no meristematic activity', `disrupted internal anatomy', and `proliferating')\\u000a were identified among torpedo- and cotyledonary-stage embryos. Among these, only the first two were found

K. Padmanabhan; D. J. Cantliffe; R. C. Harrell; D. B. McConnell

1998-01-01

392

Arbuscular mycorrhizal fungi associated with Artemisia umbelliformis Lam, an endangered aromatic species in Southern French Alps, influence plant P and essential oil contents  

Microsoft Academic Search

Root colonization by arbuscular mycorrhizal (AM) fungi of Artemisia umbelliformis, investigated in natural and cultivated sites in the Southern Alps of France, showed typical structures (arbuscules, vesicles,\\u000a hyphae) as well as spores and mycelia in its rhizosphere. Several native AM fungi belonging to different Glomeromycota genera\\u000a were identified as colonizers of A. umbelliformis roots, including Glomus tenue, Glomus intraradices, G.

Marie-Noëlle Binet; Diederik van Tuinen; Nicolas Deprêtre; Nathalie Koszela; Catherine Chambon; Silvio Gianinazzi

2011-01-01

393

Essential oil composition and antibacterial activity of Tanacetum argenteum (Lam.) Willd. ssp. argenteum and T. densum (Lab.) Schultz Bip. ssp. amani heywood from Turkey.  

PubMed

Water-distilled essential oils from aerial parts of Tanacetum argenteum ssp. argenteum and T. densum ssp. amani from Turkey were analyzed by GC and GC/MS. The essential oil of T. argenteum ssp. argenteum was characterized with alpha-pinene 36.7%, beta-pinene 27.5% and 1,8-cineole 9.8%. T. densum ssp. amani was characterized with beta-pinene 27.2%, 1,8-cineole 13.1%, alpha-pinene 9.7% and p-cymene 8.9%. Antibacterial activity of the oils were evaluated for five Gram-positive and five Gram-negative bacteria by using a broth microdilution assay. The highest inhibitory activity was observed against Bacillus cereus for T. argenteum ssp. argenteum oil (125 microg/mL) when compared with positive control chloramphenicol it showed the same inhibition potency. However, the same oil showed lower inhibitory activity against B. subtilis when compared. The oil of T. densum ssp. amani did not show significant activity against the tested microorganisms. DPPH radical scavenging activity of the T. argenteum ssp. argenteum oil was investigated for 15 and 10 mg/mL concentrations. However, the oil did not show significant activity when compared to positive control alpha-tocopherol. Both oils showed toxicity to Vibrio fischeri in the TLC-bioluminescence assay. PMID:20513969

Polato?lu, Kaan; Demirci, Fatih; Demirci, Betül; Gören, Nezhun; Ba?er, Kemal Hüsnü Can

2010-01-01

394

In vitro antimicrobial activity of the leaf extract of Harungana madagascariensis Lam. Ex Poir. (Hypericaceae) against strains causing otitis externa in dogs and cats.  

PubMed

Otitis externa in dogs and cats is always caused by a combination of yeasts and bacteria, among which the most important are Malassezia pachydermatis, Staphylococcus intermedius and Pseudomonas species. These organisms often develop resistance to classical antimicrobial agents. Therefore, the aim of this study was to investigate the antimicrobial activities of an ethyl acetate leaf extract of Harungana madagascariensis against the organisms cited, to carry out the phytochemical investigation of this extract and to determine its bioactive chemical class using dilution techniques, the bioautography method and the standard phytochemical method described by Harborne (1973). Phytochemical analysis revealed the presence of saponins, tannins, flavonoids, alkaloids and anthracenic derivatives. The bioassay showed that the antimicrobial properties may be attributed to astilbin, a flavanone derivative identified on the basis of its spectroscopic data. The results suggest that the extract could be used in an antimicrobial preparation effective against the whole range of organisms incriminated in otitis externa in dogs and cats, with a minimal inhibitory concentration (MIC) of 250 microg/ml. PMID:17385560

Moulari, B; Pellequer, Y; Chaumont, J P; Guillaume, Y C; Millet, J

2007-03-01

395

Using Haines Index coupled with fire weather model predicted from high resolution LAM forecasts to asses wildfire extreme behaviour in Southern Europe.  

NASA Astrophysics Data System (ADS)

Haines Index (HI) was developed by USDA Forest Service to measure the atmosphere's contribution to the growth potential of a wildfire. The Haines Index combines two atmospheric factors that are known to have an effect on wildfires: Stability and Dryness. As operational tools, HI proved its ability to predict plume dominated high intensity wildfires. However, since HI does not take into account the fuel continuity, composition and moisture conditions and the effects of wind and topography on fire behaviour, its use as forecasting tool should be carefully considered. In this work we propose the use of HI, predicted from HR Limited Area Model forecasts, coupled with a Fire Weather model (i.e., RISICO system) fully operational in Italy since 2003. RISICO is based on dynamic models able to represent in space and in time the effects that environment and vegetal physiology have on fuels and, in turn, on the potential behaviour of wildfires. The system automatically acquires from remote databases a thorough data-set of input information both of in situ and spatial nature. Meteorological observations, radar data, Limited Area Model weather forecasts, EO data, and fuel data are managed by a Unified Interface able to process a wide set of different data. Specific semi-physical models are used in the system to simulate the dynamics of the fuels (load and moisture contents of dead and live fuel) and the potential fire behaviour (rate of spread and linear intensity). A preliminary validation of this approach will be provided with reference to Sardinia and Corsica Islands, two major islands of the Mediterranean See frequently affected by extreme plume dominated wildfires. A time series of about 3000 wildfires burnt in Sardinia and Corsica in 2007 and 2008 will be used to evaluate the capability of HI coupled with the outputs of the Fire Weather model to forecast the actual risk in time and in space.

Gaetani, Francesco; Baptiste Filippi, Jean; Simeoni, Albert; D'Andrea, Mirko

2010-05-01

396

Larvicidal, ovicidal, and adulticidal efficacy of Erythrina indica (Lam.) (Family: Fabaceae) against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae).  

PubMed

Mosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis, and Japanese encephalitis. Mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. In view of the recently increased interest in developing plant origin insecticides as an alternative to chemical insecticide, this study was undertaken to assess the larvicidal, ovicidal, and adulticidal potential of the crude hexane, benzene, chloroform, ethyl acetate, and methanol solvent extracts from the medicinal plant Erythrina indica against the medically important mosquito vectors, Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae). The larval mortality was observed after 24 h of exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in methanol extract of leaf of E. indica against the larvae of A. stephensi, A. aegypti, and C. quinquefasciatus with the LC50 and LC90 values of 69.43, 75.13, and 91.41 ppm and 125.49, 134.31, and 167.14 ppm, respectively. The mean percent hatchability of the eggs was observed after 48 h post treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. All the five solvent extracts showed moderate ovicidal activity; however, the methanol extract showed the highest ovicidal activity. The methanol extract of E. indica against A. stephensi, A. aegypti, and C. quinquefasciatus exerted 100 % mortality (zero hatchability) at 150, 200, and 250 ppm, respectively. Control eggs showed above 99.3-100 % hatchability. The adult mortality was observed after 24 h recovery period. The plant crude extracts showed dose-dependent mortality. At higher concentrations, the adult showed restless movement for some times with abnormal wagging and then died. Among the extracts tested, the highest adulticidal activity was observed in methanol extract against A. stephensi followed by A. aegypti and C. quinquefasciatus with the LD50 and LD90 values of 88.76, 94.09, and 119.64 ppm and 160.83, 169.01, and 219.77 ppm, respectively. No mortality was recorded in the control. Our data suggest that the crude hexane, benzene, chloroform, ethyl acetate, and methanol solvent extracts of E. indica have the potential to be used as an eco-friendly approach for the control of the A. stephensi, A. aegypti, and C. quinquefasciatus. PMID:24322290

Govindarajan, Marimuthu; Sivakumar, Rajamohan

2014-02-01

397

Evaluation of anti-urolithiatic effect of aqueous extract of Bryophyllum pinnatum (Lam.) leaves using ethylene glycol-induced renal calculi  

PubMed Central

Materials and Methods : Thirty-six Wistar male rats were randomly divided into six equal groups. Group A animals received distilled water for 28 days. Group B to group F animals received 1% v/v ethylene glycol in distilled water for 28 days and group B served as ethylene glycol control. Groups C and D (preventive groups) received aqueous extract of leaves of B. pinnatum 50 and 100 mg/kg intraperitoneally, respectively for 28 days. Groups E and F (treatment groups) received aqueous extract of leaves of B. pinnatum 50 and 100 mg/kg intraperitoneally, respectively from 15th to 28th day. On days 0 and 28, 24 hrs urine samples were collected for urinary volume and urinary oxalate measurement. On day 28, blood was collected for serum creatinine and blood urea level monitoring. All animals were sacrificed and kidneys were removed, weighed, and histopathologically evaluated for calcium oxalate crystals deposition. Results: Administration of aqueous extract of leaves of B. pinnatum reduced urine oxalate level ?significantly, as compared with Group B (p<0.001). Serum creatinine and blood urea level were ?improved significantly in all aqueous extract of leaves of B. pinnatum-treated groups. Relative ?kidney weight and calcium oxalate depositions were found significantly reduced in animals ?received ABP as compared with Group B (p<0.001). ? Conclusions: B. pinnatum is effective in prevention and treatment of ethylene glycol-induced urolithiasis. PMID:25050313

Shukla, Apexa Bhanuprasad; Mandavia, Divyesh Rasikbhai; Barvaliya, Manish Jasmatbhai; Baxi, Seema Natvarlal; Tripathi, Chandrabhanu Rajkishore

2014-01-01

398

African eggplant (Solanum anguivi Lam.) fruit with bioactive polyphenolic compounds exerts in vitro antioxidant properties and inhibits Ca2+-induced mitochondrial swelling  

PubMed Central

Objective To evaluate the antioxidant and radical scavenging activities of Solanum anguivi fruit (SAG) and its possible effect on mitochondrial permeability transition pore as well as mitochondrial membrane potential (??m) isolated from rat liver. Methods Antioxidant activity of SAG was assayed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power, iron chelation and ability to inhibit lipid peroxidation in both liver and brain homogenate of rats. Also, the effect of SAG on mitochondrial membrane potential and mitochondrial swelling were determined. Identification and quantification of bioactive polyphenolics was done by HPLC-DAD. Results SAG exhibited potent and concentration dependent free radical-scavenging activity (IC50/DPPH=275.03±7.8 µg/mL). Reductive and iron chelation abilities also increase with increase in SAG concentration. SAG also inhibited peroxidation of cerebral and hepatic lipids subjected to iron oxidative assault. SAG protected against Ca2+ (110 µmol/L)-induced mitochondrial swelling and maintained the ??m. HPLC analysis revealed the presence of gallic acid [(17.54±0.04) mg/g], chlorogenic acid (21.90±0.02 mg/g), caffeic acid (16.64±0.01 mg/g), rutin [(14.71±0.03) mg/g] and quercetin [(7.39±0.05) mg/g]. Conclusions These effects could be attributed to the bioactive polyphenolic compounds present in the extract. Our results suggest that SAG extract is a potential source of natural antioxidants that may be used not only in pharmaceutical and food industry but also in the treatment of diseases associated with oxidative stress. PMID:24075339

Elekofehinti, Olusola Olalekan; Kamdem, Jean Paul; Bolingon, Aline Augusti; Athayde, Margareth Linde; Lopes, Seeger Rodrigo; Waczuk, Emily Pansera; Kade, Ige Joseph; Adanlawo, Isaac Gbadura; Rocha, Joao Batista Teixeira

2013-01-01

399

75 FR 69158 - Quarterly Publication of Individuals, Who Have Chosen To Expatriate, as Required by Section 6039G  

Federal Register 2010, 2011, 2012, 2013

...Lyndon ...................... Lam Salina Sai Lin Lam Jada Wing Yan Lang Walter Otto Lassen Lianne Marie Lau Isaac...Woehlbier Christian Herbert Woehlbier Anne Kristin Wong Jason Chong Wong Kun Wah Wong...

2010-11-10

400

25 years of NWP at INM E. Rodriguez CaminoE. Rodriguez Camino  

E-print Network

attempts (19671985).First attempts (19671985). ECMWF LAM + SMHI ANAL (19861995)ECMWF LAM + SMHI ANAL Anal (finite differences, 0.91ºx0.91 19L) + OI Anal (SMHI) (Hortal, DíazPabón, Jimeno) (SMHI) (Hortal

Vali, Gabor

401

75 FR 62462 - Additions to the List of Validated End-Users in the People's Republic of China: Hynix...  

Federal Register 2010, 2011, 2012, 2013

...Hi-Tech Development Zone, Wuhan City, Hubei Province, China 430074. Lam Research...Hi-Tech Development Zone, Wuhan City, Hubei Province, China 430223. Eligible Items...Hi-Tech Development Zone, Wuhan City, Hubei Province, China 430074. Lam...

2010-10-12

402

PharmGKB Update: II. CYP3A5, Cytochrome P450, Family 3, Subfamily A,  

E-print Network

, midazo- lam, prednisone, itraconazole, ketoconazole, fluconazole, etoposide, teniposide, vincristine, cimetidine, fluoxetine, fluvoxamine, itraconazole, keto- conazole, mibefradil, nefazodone, troleandomycin

Boguski, Mark S.

403

Ramularia pusilla Ung. and Ramulaspera holci-lanati (Cav.) Lind. in New Zealand  

Microsoft Academic Search

Ramularia pusilla Ung. was recorded on the following grasses in New Zealand:—Agrostis gigantea L., Agrostis stolonifera L., Agrostis tenuis Sibth., Anthoxanthum odoratum L., Bromus catharticus Vahl., Lolium multiflorum Lam., Lolium perenne L., Lolium perenne L. × Lolium multiflorum Lam., Lolium multiflorum Lam. × Festuca gigantea Vill., Lolium perenne L. × Festuca arundinacea Schreb., Festuca rubra L. subsp. commutata Gaud., and

G. C. M. Latch

1964-01-01

404

Laser Additive Manufacturing and Bionics: Redefining Lightweight Design  

Microsoft Academic Search

New layer wise manufacturing technologies such as Laser Additive Manufacturing (LAM) allow innovative approaches to product design. Especially for lightweight design in aircraft applications LAM offers new possibilities for load-adapted structures. However, to fully capture lightweight potential of LAM technologies new design guidelines and processes have to be developed. A novel approach to extreme lightweight design is realized by incorporating

C. Emmelmann; P. Sander; J. Kranz; E. Wycisk

2011-01-01

405

Menstrual cycle variation of retroperitoneal lymphangioleiomyomas in lymphangioleiomyomatosis.  

PubMed

Lymphangioleiomyomatosis (LAM) is a rare disease affecting women, classically involving the lungs. However, extrapulmonary manifestations also occur, including renal angiomyolipomas, retroperitoneal lymphangioleiomyomas and extrathoracic lymphadenopathy. The lung disease is hormone-responsive, but no information exists regarding sex hormone responsiveness of abdominal LAM. Here, we report two women with LAM whose abdominal lymphangioleiomyomas increased in size with hormonal changes of the menstrual cycle. This is the first description of abdominal lymphangioleiomyomas exhibiting hormone responsiveness in LAM. We describe these cases and summarize the literature on abdominal LAM. Menstrual cycle variation should be taken into account when assessing response to therapy, both clinically and in research studies. PMID:22151950

Sandrini, A; Silverstone, E; Yates, D H

2011-12-01

406

Molecular characterization of Capra hircus lysosomal ?-mannosidase and potential mutant site for the therapy of locoweed poisoning.  

PubMed

Lysosomal ?-Mannosidase (LAM) belongs to the glycoside hydrolyzing enzymes family 38 and is involved in the biosynthesis and turnover of N-linked glycoproteins process. Locoweeds, which contain swainsonine (SW) that inhibits LAM, are the main poisoning plants in many regions of the world, and thereby resulting in animal poisoning or even death. Based on regions of protein sequence conservation between LAM from Bos taurus and Homo sapiens, we cloned cDNA encoding Capra hircus LAM (chLAM). Expression of cDNA in Pichia pastoris resulted in the secretion of aLAM activity into the culture medium. The recombinant chLAM was activated 1.6 and 1.2-fold with Zn(2+) and Ca(2+), respectively. By homology modeling, molecular docking and mutant analysis, we obtained the probable binding modes of SW at the allosteric sites of chLAM, and the potential mutant sites for the resistance to SW. Prediction of SW sensitivity to A28 W/G, D58 Y/G mutant chLAM is lower than wild type chLAM. The obtained results lead to a better understanding of not only interactions between substrate/SW and chLAM, but also of a potential strategy for a novel therapy for locoweed poisoning. PMID:24660168

Xiangya, Kong; Jiangye, Zhang; Ying, Wu; Jianfei, Li; Qinfan, Li

2014-01-01

407

Study of $?- \\Lambdabar$ Oscillation in quantum coherent $?\\Lambdabar$ state by using J/?\\to ?\\bar?$ decay  

E-print Network

We discuss the possibility of searching for the $\\Lam - \\Lamb$ oscillations for coherent $\\Lam\\Lamb$ production in the J/$\\psi \\to \\Lam \\Lamb$ decay process. The sensitivity of measurement of $\\Lam -\\Lamb$ oscillation in the external field at BES-III experiment is considered. These considerations indicate an alternative way to probe the $\\Delta B =2$ amplitude in addition to neutron oscillation experiments. Both coherent and time-dependent information can be used to extract the $\\Lam-\\Lamb$ oscillation parameter. With one year's luminosity at BES-III, we can set an upper limit of $\\delta m_{\\Lam \\Lamb} < 10^{-15}$ MeV at 90% confidence level, corresponding to about $10^{-6}$ s of $\\Lam-\\Lamb$ oscillation time.

Xian-Wei Kang; Hai-Bo Li; Gong-Ru Lu

2009-06-01

408

Integrating the Lactational Amenorrhea Method into a family planning program in Ecuador.  

PubMed

This paper reports the results of a 12-month implementation study documenting the process of integrating the Lactational Amenorrhea Method (LAM) into a multiple-method family planning service-delivery organization, the Céntro Médico de Orientación y Planificación Familiar (CEMOPLAF), in Ecuador. LAM was introduced as a family planning option in four CEMOPLAF clinics. LAM was accepted by 133 breastfeeding women during the program's first five months, representing about one-third of postpartum clients. Seventy-three percent of LAM acceptors were new to any family planning method. Follow-up interviews with a systematic sample of 67 LAM users revealed that the method was generally used correctly. Three pregnancies were reported, none by women who were following LAM as recommended. Service providers' knowledge of LAM resulted in earlier IUD insertions among breastfeeding women. Relationships with other maternal and child health organizations and programs were also established. PMID:7940621

Wade, K B; Sevilla, F; Labbok, M H

1994-01-01

409

An agr-Like Two-Component Regulatory System in Lactobacillus plantarum Is Involved in Production of a Novel Cyclic Peptide and Regulation of Adherence  

PubMed Central

We have analyzed a locus on the annotated Lactobacillus plantarum WCFS1 genome that showed homology to the staphylococcal agr quorum-sensing system and designated it lam for Lactobacillus agr-like module. Production of the lamBDCA transcript was shown to be growth phase dependent. Analysis of a response regulator-defective mutant (?lamA) in an adherence assay showed that lam regulates adherence of L. plantarum to a glass surface. Global transcription analysis of the wild-type and ?lamA strains in early, mid-, and late log phase of growth was performed using a clone-based microarray. Remarkably, only a small set of genes showed significant differences in transcription profiles between the wild-type and lamA mutant strains. The microarray analysis confirmed that lamBDCA is autoregulatory and showed that lamA is involved in regulation of expression of genes encoding surface polysaccharides, cell membrane proteins, and sugar utilization proteins. The lamBD genes encoding the putative autoinducing peptide precursor (LamD) and its processing protein (LamB) were overexpressed using the nisin-controlled expression system, and culture supernatants were analyzed by liquid chromatography/mass spectrometry (LC/MS) to identify overproduced LamD-derived peptides. In this way, a cyclic thiolactone pentapeptide that possesses a ring structure similar to those of autoinducing peptides of the staphylococcal agr system was identified. The peptide was designated LamD558, and its sequence (CVGIW) matched the annotated precursor peptide sequence. Time course analysis of wild-type culture supernatants by LC/MS indicated that LamD558 production was increased markedly from mid-log to late log growth phase. This is the first example of an agr-like system in nonpathogenic bacteria that encodes a cyclic thiolactone autoinducing peptide and is involved in regulation of adherence. PMID:16030216

Sturme, Mark H. J.; Nakayama, Jiro; Molenaar, Douwe; Murakami, Yoshiko; Kunugi, Ryoko; Fujii, Toshio; Vaughan, Elaine E.; Kleerebezem, Michiel; de Vos, Willem M.

2005-01-01

410

YAMUK KES?TL? KANAL ?ÇER?S?NDE LAM?NER AKI?TA H?DROD?NAM?K VE ISIL OLARAK GEL??MEKTE OLAN ISI TRANSFER? PROBLEM?N?N SAYISAL OLARAK ?NCELENMES?  

Microsoft Academic Search

Laminar forced convection inside trapezoidal duct is of interest in design of thermal equipment. Heat transfer to both hydrodynamically and thermally developing three dimensional forced convection inside a duct having trapezoidal cross section was investigated numerically under steady-state and constant surface temperature conditions. Numerical study was carried out for Reynolds number range of 100?Re?800. Air (Pr?0.7) was used as working

Nevzat ONUR; Kamil ARSLAN; Feyza GÜNBEY

411

Successes in conserving the Barberry Carpet moth Pareulype berberata (D. & S.) (Geometridae) in England  

Microsoft Academic Search

Since the end of the 19th century the Barberry Carpet moth (Pareulype berberata) has declined from being widespread and fairly well distributed in England to highly localised and endangered, due mainly to large-scale removal of the sole larval foodplant Berberis vulgaris. In the 1980s the moth appeared to be restricted to a single site. Since 1987 the moth has been

Paul Waring

2004-01-01

412

Potential of fungi for the biological control of some New Zealand weeds  

Microsoft Academic Search

The potential for fungi to control 14 important weeds in New Zealand is reviewed. Information provided includes the fungi already known from these weeds in New Zealand, fungi recorded from the native ranges of the weeds, and past work on the control of these weeds using fungi, both in New Zealand and elsewhere. The 14 weeds are: Berberis spp.; Buddleja

Peter R. Johnston

1990-01-01

413

Environmentally Friendly Natural Dyeing of Organic Cotton  

Microsoft Academic Search

In this study, organic cotton fabrics were dyed with different natural dye sources (madder root, walnut shell, henna, horse chestnut, pomegranate peel, berberis vulgaris root, thyme, and sage tea). The dyeing was carried out with different mordants (copper sulphate, potassium aluminum sulphate, potassium tartrate, and citric acid), using pre-mordanting dyeing methods. The color of the fabrics was investigated in terms

Mustafa Tutak; N. Ebru Korkmaz

2012-01-01

414

Improved detection of alkaloids in crude extracts applying capillary electrophoresis with field amplified sample injection  

Microsoft Academic Search

A simple and effective method for the sensitive detection of alkaloids in crude plant extracts applying capillary electrophoresis with field amplified sample injection (FASI) is described. This method was compared with normal pressure injection for the determination of alkaloids in methanolic extracts from roots of Berberis vulgaris L. (Berberidaceae) and Hydrastis canadensis L. (Ranunculaceae) using a 1:1 mixture of 200

Matthias Unger; Joachim Stöckigt

1997-01-01

415

Fertilisation of the Barberry  

Microsoft Academic Search

C. K. SPRENGEL, in his Entdeckte Geheimniss der Natur im Bau und in der Befruchtung der Blumen, gives an excellent account of the structure of the Common Barberry, Berberis vulgaris, and points out how it is visited by insects, and how, upon the touch of an insect's limb or proboscis, the irritable filaments move inwards, and press the opened anthers

T. H. Farrer

1870-01-01

416

First enantioselective total synthesis of (-)-tejedine.  

PubMed

[structure: see text] The first enantioselective total synthesis of (-)-tejedine (1) is reported. Tejedine is a seco-bisbenzyltetrahydroisoquinoline isolated in 1998 as a minor component from Berberis vulgaris. The synthesis was achieved using a strategy employing four key steps, including a chiral auxiliary-assisted diastereoselective Bischler-Napieralski cyclization. PMID:12153207

Wang, You-Chu; Georghiou, Paris E

2002-08-01

417

EFFICACY OF BARBERRY AQUEOUS EXTRACTS DENTAL GEL ON CONTROL OF PLAQUE AND GINGIVITIS  

Microsoft Academic Search

Herbal extracts have been successfully used in dentistry as tooth cleaning and antimicrobial plaque agents. The aim of this study was to evaluate the clinical effects of a dental gel containing barberry extracts (from Berberis vulgaris) on gingivitis and microbial plaque control. A double blind clinical trial study was conducted in a dormitory on 45 boys aged 11-12 years having

A. Makarem; N. Khalili; R. Asodeh

418

Adaptation of wheat rusts to the wheat cultivars in former Czechoslovakia  

Microsoft Academic Search

In former Czechoslovakia virulence of rusts attacking wheat was studied since the sixties. Since the same time genes for resistance in the registered cultivars were identified. The role of Berberis and Thalictrum as alternate hosts for stem rust and leaf rust, respectively, was investigated as well. Determined changes of virulence in rust populations could only partially be ascribed to changes

Pavel Bartoš; Eva Stuchlíková; Renata Hanušová

1996-01-01

419

Prevalence and Distribution of Common Barberry, the Alternate Host of Puccinia graminis , in Minnesota  

Microsoft Academic Search

Peterson, P. D., Leonard, K. J., Miller, J. D., Laudon, R. J., and Sutton, T. B. 2005. Prevalence and distribution of common barberry, the alternate host of Puccinia graminis, in Minnesota. Plant Dis. 89:159-163. A federal and state program operated from 1918 until the 1980s to eradicate common barberry (Berberis vulgaris), the alternate host of Puccinia graminis, from the major

P. D. Peterson; K. J. Leonard; J. D. Miller; R. J. Laudon; T. B. Sutton

2005-01-01

420

Differences in Earthworm Densities and Nitrogen Dynamics in Soils Under Exotic and Native Plant Species  

Microsoft Academic Search

Previous studies of the invasion of two exotic plants – Berberis thunbergii and Microstegium vimineum – in hardwood forests of New Jersey have shown a significant increase of pH in soils under the invasive plants as compared with soils from under native shrubs (Vaccinium spp). We present a further investigation of soil properties under the exotic plants in question. We

P. S. Kourtev; W. Z. Huang; J. G. Ehrenfeld

1999-01-01

421

75 FR 29191 - Black Stem Rust; Additions of Rust-Resistant Varieties  

Federal Register 2010, 2011, 2012, 2013

...United States. The disease is caused by a fungus that reduces the quality and yield of...addition to infecting small grains, the fungus lives on a variety of alternate host plants...Berberis, Mahoberberis, and Mahonia. The fungus is spread from host to host by...

2010-05-25

422

78 FR 27855 - Black Stem Rust; Additions of Rust-Resistant Species and Varieties  

Federal Register 2010, 2011, 2012, 2013

...United States. The disease is caused by a fungus (Puccinia graminis) that reduces the...addition to infecting small grains, the fungus lives on a variety of alternate host plants...Berberis, Mahoberberis, and Mahonia. The fungus is spread from host to host by...

2013-05-13

423

76 FR 13970 - Notice of Request for Extension of Approval of an Information Collection; Black Stem Rust...  

Federal Register 2010, 2011, 2012, 2013

...United States. The disease is caused by a fungus that reduces the quality and yield of...addition to infecting small grains, the fungus lives on a variety of alternate host plants...Berberis, Mahoberberis, and Mahonia. The fungus is spread from host to host by...

2011-03-15

424

75 FR 54461 - Black Stem Rust; Additions of Rust-Resistant Varieties  

Federal Register 2010, 2011, 2012, 2013

...United States. The disease is caused by a fungus that reduces the quality and yield of...addition to infecting small grains, the fungus lives on a variety of alternate host plants...Berberis, Mahoberberis, and Mahonia. The fungus is spread from host to host by...

2010-09-08

425

IR-4 Ornamental Horticulture Program Research Report Form  

E-print Network

. The Berberis plants grew only slightly during the course of the trial. The control plants increased in height decreases in height which were not significantly different from the control increase. The decreases in width was noted for height, width or volume increase (Tables 2 and 6c, Figure 3c). Biathlon can be considered safe

Lieth, J. Heinrich

426

IR-4 Ornamental Horticulture Program Research Report Form  

E-print Network

, Figures 1 and 2b). At weeks 6, 7 and 8 these differences were significant at 10%. Some plants in all group and plants treated with Freehand (Tables 1 and 6c, Figure 2c). Plant Growth. No significant aquifolium (Berberis aquifolium) Phytotoxicity. There were no significant differences in phytotoxicity

Lieth, J. Heinrich

427

INVASIVE PLANTS AND THE GREEN INDUSTRY  

Microsoft Academic Search

There are many motivations for introducing plant species to areas outside their native range. Non-native plants can provide food, medicine, shelter, and ecosystem services, as well as aesthetic value. However, some species, such as Norway maple (Acer platanoides), Japanese barberry (Berberis thunbergii), and Oriental bittersweet (Celastrus orbiculatus), have escaped from cultivation, with severe ecological and economic consequences. The approval of

Robin A. Harrington; Ronald Kujawski; H. Dennis; P. Ryan

428

Carbon Dioxide Exchange by Several Streamside and Scrub Oak Community Species of Red Butte Canyon, Utah  

Microsoft Academic Search

ABSTRACT: Species of a xeric scrub oak community (scrub oak, Quercus gambelii; sagebrush, Artemisia tridentata; bitterbrush, Purshia have lower seasonal water potentials than species of a mesic streamside habitat (box elder, Acer negundo; bigtooth maple, Acer grandidentatum; creeping barberry, Berberis repens). The restriction of box elder to mesic sites is, in part, a function of its physiological responses to moisture

Stephen J. Dina; Lionel G. Klikoff

2007-01-01

429

2009 Virginia Polytechnic Institute and State University 2901-1050 Virginia Cooperative Extension programs and employment are open to all, regardless of race, color, national origin, sex, religion, age, disability, political beliefs, sexual orientation, o  

E-print Network

, Administrator, 1890 Extension Program, Virginia State, Petersburg. Japanese Barberry (Berberis thunbergii var barberry has thorns which may be an advantage (deer proof, pedestrian traffic control) or a liability later in season. #12;2 Related species that have not shown invasive tendencies: Wintergreen Barberry

Liskiewicz, Maciej

430

Four Cultivars of Japanese Barberry Demonstrate Differential Reproductive Potential under Landscape Conditions  

Microsoft Academic Search

While japanese barberry (Berberis thunbergii DC.) is an acknowledged inva- sive plant naturalized throughout the eastern and northern U.S., the danger posed by its popular horticultural forms is unknown and controversial. This work analyzed the reproductive potential and seedling growth of four ornamental genotypes important to the nursery industry. Fruit and seed production was quantified in 2001, 2002, and 2003

Jonathan M. Lehrer; Mark H. Brand; Jessica D. Lubell

2006-01-01

431

[Ability of typical greenery shrubs of Beijing to adsorb and arrest PM2.5 ].  

PubMed

Four typical types of green shrubs of Beijing (Euonymus japonicus, Buxus microphylla, Berberis thunbergii cv. atropurpurea, Taxus cuspidate cv. nana) were selected to study their capacities in adsorbing and arresting PM2.5 using both field observations and air chamber simulations. Concurrently, in order to analyze the pollution characteristics of Beijing in winter and spring, the PM2.5 concentrations of December 2012 to May 2013 were collected. Experimental results showed that: From the gas chamber experiments, the ability to adsorb and arrest PM2.5 was in the order of Berberis thunbergii cv. atropurpurea > Buxus microphylla > Taxus cuspidate cv. nana > Euonymus japonicus, mainly due to the differences in leaf characteristics; Outside measurement results showed that the ability to adsorb and arrest PM2.5 was ranked as Buxus microphylla > Berberis thunbergii cv. atropurpurea > Taxus cuspidate cv. nana > Euonymus japonicus. Chamber simulation and outdoor observation showed that Buxus microphylla and Berberis thunbergii cv. atropurpurea had strong ability to adsorb and arrest PM2.5; Meanwhile, the slight differences between the chamber simulation and outdoor observation results might be related to plant structure. Compared to tree species, the planting condition of shrub species was loose, and it greened quickly; By analyzing the Beijing PM2.5 concentration values in winter and spring, it was found that the PM2.5 concentration was particularly high in the winter of Beijing, and evergreen shrubs maintained the ability to adsorb and arrest PM2.5. PMID:25518685

Liang, Dan; Wang, Bin; Wang, Yun-qi; Zhang, Hui-lan; Yang, Song-nan; Li, Ang

2014-09-01

432

Heterologous expression of alkaloid biosynthetic genes — a review  

Microsoft Academic Search

Tetrahydrobenzylisoquinoline alkaloids comprise a diverse class of secondary metabolites with many pharmacologically active members. The biosynthesis at the enzyme level of at least two tetrahydrobenzylisoquinoline alkaloids, the benzophenanthridine alkaloid sanguinarine in the California poppy, Eschscholtzia californica, and the bisbenzylisoquinoline alkaloid berbamunine in barberry, Berberis stolonifera, has been elucidated in detail starting from the aromatic amino acid (aa) l-tyrosine. In an

Toni M. Kutchan

1996-01-01

433

A S E R I O U S T H R E A T Iowa's Woodlands  

E-print Network

A S E R I O U S T H R E A T To Iowa's Woodlands Japanese Barberry Berberis thunbergiiJan Feb March by changing the soil chemistry. What is Japanese Barberry? · A short deciduous shrub. · Native to Asia

US Army Corps of Engineers

434

INVASIVE SHRUBS AND SONGBIRD NESTING SUCCESS: EFFECTS OF CLIMATE VARIABILITY AND PREDATOR ABUNDANCE  

Microsoft Academic Search

Previous studies have demonstrated that songbirds often use exotic plants as nesting substrates and may suffer elevated predation rates relative to nests placed in native plants. Veeries (Catharus fuscescens) frequently build nests in an exotic shrub, Japanese barberry (Berberis thunbergii), in forests of southeastern New York State, USA. We monitored Veery nesting success over a six-year period and supplemented these

Kenneth A. Schmidt; Lisa C. Nelis; Nathan Briggs; Richard S. Ostfeld

2005-01-01

435

Possible involvement of l-arginine-nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) signaling pathway in the antidepressant activity of berberine chloride  

Microsoft Academic Search

Berberine is an isoquinoline alkaloid isolated from Berberis aristata, a major herb widely used in Indian and Chinese systems of medicine. Berberine possessed a wide range of biological activity including antidiarrheal, antimicrobial, anti-inflammatory effects and some central nervous system activity as well. The present study was designed to explore the antidepressant activity and its possible mechanism of action. Further, the

Shrinivas K. Kulkarni; Ashish Dhir

2007-01-01

436

Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents  

Microsoft Academic Search

Berberis aristata is an edible plant employed in the South Asian Traditional Medicine, particularly its fruits being used as a tonic remedy for liver and heart. In this investigation, berberine, a known compound from this plant, was studied for its possible antihepatotoxic action in rats. Pretreatment of animals with berberine (4 mg\\/kg; orally twice daily for 2 days) prevented the

K. H Janbaz; A. H Gilani

2000-01-01

437

Effect of Berberine Sulphate on Entamoeba histolytica  

Microsoft Academic Search

BERBERINE, an alkaloid derived from the plant Berberis aristata Linn., has been shown to be useful in the treatment of experimental cholera in the infant rabbit model1. It compares well with chloramphenicol in the chemotherapy of cholera and severe diarrhoea in humans2. Results of the investigations on Entamoeba histolytica reported here suggest that berberine and its salts, such as berberine

T. V. Subbaiah; A. H. Amin

1967-01-01

438

The maltoporin of Salmonella typhimurium: sequence and folding model.  

PubMed

The sequence of the lamB gene from Salmonella typhimurium was determined. It encodes the precursor to the LamB protein from S. typhimurium (pre-LamBS.t.; 452 residues) which presents extensive homologies with the pre-LamB protein from Escherichia coli (pre-LamBE.c.; 446 residues). The first third of pre-LamBS.t. is the most conserved, with 4% changes and strict identity between the signal peptides. The last two-third contains five "variable" segments where more than 50% of the residues are changed with respect to LamBE.c.. The three first variable segments are 8 to 14 residues long and contain only substitutions, while the two more distal ones are 24 and 29 residues long and also include insertions and deletions. It is remarkable that the variable segments correspond essentially to regions predicted to be extramembranous loops on our 2D folding model for LamBE.c.; they alternate with conserved predicted transmembranous segments. Four of the variable regions were predicted to be cell-surface-exposed loops on the basis of genetic and immunological data, while one of them (region II) was predicted to be periplasmic on the sole basis of folding rules. The LamB protein from S. typhimurium can substitute for the LamB protein from E. coli for maltodextrins binding and transport, but not for infection by any of the known E. coli phages using LamBE.c. for adsorption. A tetrapeptide, RGDS, assumed to be responsible for mammalian cell aggregation by LamBE.c. is conserved in LamBS.t., suggesting that it could have a functional role. The conservation of the binding and transport activity can be accounted for by the conservation of the regions known to be directly involved, namely the first third of the protein and a region corresponding to 352 to 374 of LamBS.t.. The phage resistance can be attributed to the variability of the four cell-surface-exposed loops previously identified as essential for phage adsorption. These results, together with those obtained with polyclonal and monoclonal antibodies directed against known LamB regions, strongly support the folding model presented for LamBE.c. and the idea that it can essentially be extended to LamBS.t., except perhaps for a region between residues 155 and 245. We propose that the existence of variable regions is due essentially, and perhaps only, to the local lack of structural constraints in the protein. The intergenic region between lamB and the following gene, malM, comprises conserved segments, including one palindromic unit. PMID:2092357

Francoz, E; Molla, A; Dassa, E; Saurin, W; Hofnung, M

1990-01-01

439

Sulfated modification and anti-tumor activity of laminarin  

PubMed Central

The aim of this study was to investigate the sulfated modification of laminarin and the changes in structure and antitumor activity. The chlorosulfonic acid-pyridine method was applied for sulfated modification. The molecular weights of laminarin and laminarin sulfate (LAMS) were measured by high-performance liquid chromatography (HPLC), and IR and NMR spectra were also recorded. The surface conformations of laminarin and LAMS were observed with a scanning electron microscope. The antitumor activities of the two polysaccharides were also evaluated using an MTT assay. LAMS with a sulfate content of 45.92% and a molecular weight of 16,000 was synthesized. The IR spectra of laminarin and LAMS showed the characteristic absorption peaks of a polysaccharide, and LAMS also had the characteristic absorption peaks of sulfate moieties. The NMR spectra showed that laminarin and LAMS had ?-(1?3) glycosidic bonds forming the main chain, and sulfate substitution was at the hydroxyl groups of C2 and C6. Under the scanning electron microscope, there were clear differences in surface conformation between laminarin and LAMS; laminarin was cloud-like and spongy, while LAMS was block-like and flaky. The MTT results showed that laminarin and LAMS had inhibitory effects on LoVo cell growth, and the antitumor activity of LAMS was higher than that of laminarin at the same concentration. This suggests that sulfated modification was able to change the laminarin structure and markedly enhance the antitumor activity. PMID:24223655

JI, CHEN-FENG; JI, YU-BIN; MENG, DE-YOU

2013-01-01

440

Lymphangioleiomyomatosis — a wolf in sheep’s clothing  

PubMed Central

Lymphangioleiomyomatosis (LAM) is a rare progressive lung disease of women. LAM is caused by mutations in the tuberous sclerosis genes, resulting in activation of the mTOR complex 1 signaling network. Over the past 11 years, there has been remarkable progress in the understanding of LAM and rapid translation of this knowledge to an effective therapy. LAM pathogenic mechanisms mirror those of many forms of human cancer, including mutation, metabolic reprogramming, inappropriate growth and survival, metastasis via blood and lymphatic circulation, infiltration/invasion, sex steroid sensitivity, and local and remote tissue destruction. However, the smooth muscle cell that metastasizes, infiltrates, and destroys the lung in LAM arises from an unknown source and has an innocent histological appearance, with little evidence of proliferation. Thus, LAM is as an elegant, monogenic model of neoplasia, defying categorization as either benign or malignant. PMID:23114603

Henske, Elizabeth P.; McCormack, Francis X.

2012-01-01

441

Chyloperitoneum, chylothorax and lower extremity lymphedema in woman with sporadic lymphangioleiomyomatosis successfully treated with sirolimus: a case report.  

PubMed

Lymphangioleiomyomatosis (LAM) is a rare disease characterized by diffuse thin-walled cysts throughout the lungs on computed tomography and diffuse proliferation of abnormal smooth muscle-like cells (LAM cells) on lung biopsy. LAM affects women almost exclusively, predominantly in their reproductive age. The most typical presenting symptoms include dyspnea, spontaneous pneumothorax, cough and chylothorax. Abdominal findings represent less common initial manifestations of the disease and may pose diagnostic difficulties. The treatment of LAM has not been fully established. Recent studies report effectiveness of sirolimus in LAM patients. We report the case of a 45-year-old woman with sporadic LAM, successfully treated with sirolimus, in whom the first manifestation of the disease was chyloperitoneum and after three and nine years, respectively, lymphedema of the left lower extremity and right sided chylothorax occurred. PMID:23057149

Chachaj, A; Drozdz, K; Chabowski, M; Dziegiel, P; Grzegorek, I; Wojnar, A; Jazwiec, P; Szuba, A

2012-06-01

442

Impact of nesting strategies in dynamical downscaling of reanalysis data  

Microsoft Academic Search

Coarse-grid global numerical weather simulations or analysis data have to be downscaled, e.g., with nested limited-area models (LAMs), for regional interpretation. Here, the impact of different one-way nesting strategies on precipitation simulations over the European Alps with the LAM ALADIN is studied. The LAM is forced by initial and lateral boundary data derived from ERA40 reanalyses with 120 km horizontal

A. Beck; B. Ahrens; K. Stadlbacher

2004-01-01

443

Impact of nesting strategies in dynamical downscaling of reanalysis data  

Microsoft Academic Search

Coarse–grid global numerical weather simulations or analysis data have to be downscaled, e.g., with nested limited–area models (LAMs), for regional interpretation. Here, the impact of different one–way nesting strategies on precipitation simulations over the European Alps with the LAM ALADIN is studied. The LAM is forced by initial and lateral boundary data derived from ERA40 reanalyses with 120 km horizontal

A. Beck; B. Ahrens; K. Stadlbacher

2004-01-01

444

1995-2011 | LS-LAMP IMPACT REPORT Louis stokes  

E-print Network

LA BAGAyoko Ls-LAmP Pi and Project Director Dr. LuriA younG Ls-LAmP co-Pi Dr. eLLA L. keLLy Ls-LAmP co-Pi #12........................................20 Ls-LaMP Partner highLights...................................21 DiLLarD universityLA BAGAyoko, Ls-LaMP Project Director Dear Readers, The Louis Stokes Louisiana Alliance for Minority

Kulp, Mark

445

Prior Exposure to Lamivudine Increases Entecavir Resistance Risk in Chronic Hepatitis B Patients without Detectable Lamivudine Resistance  

PubMed Central

The efficacy of entecavir (ETV) treatment in chronic hepatitis B (CHB) patients who were exposed to lamivudine (LAM) but had no detectable LAM resistance (LAM-R) is not well evaluated. In this study, we aimed to evaluate whether the probability of developing genotypic resistance to ETV in LAM-exposed patients with or without LAM-R is comparable to that in antiviral-naive patients. This retrospective cohort study included 500 consecutive patients with CHB who started ETV monotherapy at a single tertiary hospital in Korea. The patients were divided into three groups: nucleos(t)ide analogue (NA)-naive patients (group 1, n = 142), patients who were previously exposed to LAM and had no currently or previously detected LAM-R (group 2, n = 233), and patients with LAM-R when starting ETV (group 3, n = 125). The overall median ETV treatment duration was 48.7 months. The probabilities of virologic breakthrough were significantly increased not only in group 3 (hazard ratio [HR] = 14.4, P < 0.001) but also in group 2 (HR = 5.0, P < 0.001) compared to group 1. Genotypic ETV resistance (ETV-R) developed more frequently in group 2 (HR = 13.0, P = 0.013) as well as group 3 (HR = 43.9, P < 0.001) than in group 1: the probabilities of developing ETV-R in groups 1, 2, and 3 were <1.0%, 8.0%, and 28.2%, respectively, at month 48. The results of this study indicate that ETV-R occurred more frequently in LAM-exposed patients, even though they had no detectable LAM-R, than in NA-naive patients. Therefore, LAM-exposed CHB patients, regardless of the presence or absence of LAM-R, should be monitored more cautiously for the development of ETV-R during ETV monotherapy. PMID:24395227

Lee, Jeong-Hoon; Cho, Yuri; Lee, Dong Hyeon; Lee, Minjong; Yoo, Jeong-ju; Choi, Won-mook; Cho, Young Youn; Lee, Yun Bin; Yu, Su Jong; Yoon, Jung-Hwan; Lee, Hyo-Suk

2014-01-01

446

The neural crest lineage as a driver of disease heterogeneity in Tuberous Sclerosis Complex and Lymphangioleiomyomatosis  

PubMed Central

Lymphangioleiomyomatosis (LAM) is a rare neoplastic disease, best characterized by the formation of proliferative nodules that express smooth muscle and melanocytic antigens within the lung parenchyma, leading to progressive destruction of lung tissue and function. The pathological basis of LAM is associated with Tuberous Sclerosis Complex (TSC), a multi-system disorder marked by low-grade tumors in the brain, kidneys, heart, eyes, lung and skin, arising from inherited or spontaneous germ-line mutations in either of the TSC1 or TSC2 genes. LAM can develop either in a patient with TSC (TSC-LAM) or spontaneously (S-LAM), and it is clear that the majority of LAM lesions of both forms are characterized by an inactivating mutation in either TSC1 or TSC2, as in TSC. Despite this genetic commonality, there is considerable heterogeneity in the tumor spectrum of TSC and LAM patients, the basis for which is currently unknown. There is extensive clinical evidence to suggest that the cell of origin for LAM, as well as many of the TSC-associated tumors, is a neural crest cell, a highly migratory cell type with extensive multi-lineage potential. Here we explore the hypothesis that the types of tumors that develop and the tissues that are affected in TSC and LAM are dictated by the developmental timing of TSC gene mutations, which determines the identities of the affected cell types and the size of downstream populations that acquire a mutation. We further discuss the evidence to support a neural crest origin for LAM and TSC tumors, and propose approaches for generating humanized models of TSC and LAM that will allow cell of origin theories to be experimentally tested. Identifying the cell of origin and developing appropriate humanized models is necessary to truly understand LAM and TSC pathology and to establish effective and long-lasting therapeutic approaches for these patients. PMID:25505789

Delaney, Sean P.; Julian, Lisa M.; Stanford, William L.

2014-01-01

447

The laser additive manufacture of Ti6Al4V  

Microsoft Academic Search

Laser additive manufacturing (LAM) is a manufacturing technique with cost-reduction potential for titanium aerospace components.\\u000a The mechanical properties of LAM Ti-6Al-4V have been investigated extensively, but little work on microstructure evolution\\u000a has been performed to date. The results presented here provide a first look at the relationships between LAM processing parameters\\u000a and microstructure in as-deposited Ti-6Al-4V.

P. A. Kobryn; S. L. Semiatin

2001-01-01

448

The laser additive manufacture of Ti6Al4V  

Microsoft Academic Search

Laser additive manufacturing (LAM) is a manufacturing technique with cost-reduction potential for titanium aerospace components. The mechanical properties of LAM Ti-6Al-4V have been investigated extensively, but little work on microstructure evolution has been performed to date. The results presented here provide a first look at the relationships between LAM processing parameters and microstructure in as-deposited Ti-6Al-4V.

P. A. Kobryn; S. L. Semiatin

2001-01-01

449

Effusion cytology: an effective method for the diagnosis of pulmonary lymphangioleiomyomatosis.  

PubMed

Lymphangioleiomyomatosis (LAM) is a rare progressive lung disease. Chylous effusion is one of the most common clinical manifestations of LAM. Herein we present a 39-year-old female who presented progressive dyspnea on exertion and chylothorax. The chest computed tomography showed multiple thin-walled cysts in both lungs which suggesting LAM. The pleural effusion cytology plus with further immunocytochemistry confirmed the computed tomography diagnosis. Therefore, the LAM can be diagnosed by cytologic examination combined with conventional chest computed tomography and clinical manifestations, which can help some patients to avoid an invasive biopsy. PMID:24822126

Fan, Daming; Ding, Li; Liu, Hui; Wang, Jigang; Ran, Wenwen; Li, Yujun; Lin, Dongliang

2014-05-01

450

Effusion cytology: an effective method for the diagnosis of pulmonary lymphangioleiomyomatosis  

PubMed Central

Lymphangioleiomyomatosis (LAM) is a rare progressive lung disease. Chylous effusion is one of the most common clinical manifestations of LAM. Herein we present a 39-year-old female who presented progressive dyspnea on exertion and chylothorax. The chest computed tomography showed multiple thin-walled cysts in both lungs which suggesting LAM. The pleural effusion cytology plus with further immunocytochemistry confirmed the computed tomography diagnosis. Therefore, the LAM can be diagnosed by cytologic examination combined with conventional chest computed tomography and clinical manifestations, which can help some patients to avoid an invasive biopsy. PMID:24822126

Fan, Daming; Ding, Li; Liu, Hui; Wang, Jigang; Ran, Wenwen; Li, Yujun

2014-01-01

451

21 CFR 73.2190 - Henna.  

...color additive henna is the dried leaf and petiole of Lawsonia alba Lam. (Lawsonia inermis L.). It may be identified by...not contain more than 10 percent of plant material from Lawsonia alba Lam. (Lawsonia inermis L.) other than the leaf...

2014-04-01

452

21 CFR 73.2190 - Henna.  

Code of Federal Regulations, 2013 CFR

...color additive henna is the dried leaf and petiole of Lawsonia alba Lam. (Lawsonia inermis L.). It may be identified by...not contain more than 10 percent of plant material from Lawsonia alba Lam. (Lawsonia inermis L.) other than the leaf...

2013-04-01

453

21 CFR 73.2190 - Henna.  

Code of Federal Regulations, 2011 CFR

...color additive henna is the dried leaf and petiole of Lawsonia alba Lam. (Lawsonia inermis L.). It may be identified by...not contain more than 10 percent of plant material from Lawsonia alba Lam. (Lawsonia inermis L.) other than the leaf...

2011-04-01

454

21 CFR 73.2190 - Henna.  

Code of Federal Regulations, 2012 CFR

...color additive henna is the dried leaf and petiole of Lawsonia alba Lam. (Lawsonia inermis L.). It may be identified by...not contain more than 10 percent of plant material from Lawsonia alba Lam. (Lawsonia inermis L.) other than the leaf...

2012-04-01

455

21 CFR 73.2190 - Henna.  

Code of Federal Regulations, 2010 CFR

...color additive henna is the dried leaf and petiole of Lawsonia alba Lam. (Lawsonia inermis L.). It may be identified by...not contain more than 10 percent of plant material from Lawsonia alba Lam. (Lawsonia inermis L.) other than the leaf...

2010-04-01

456

Nonlinear parameter estimation by linear association: application to a five-parameter passive neuron model.  

PubMed

Linear associative memories (LAM) have been intensely used in the areas of pattern recognition and parallel processing for the past two decades. Application of LAM to nonlinear parameter estimation, however, has only been recently attempted. The process consists in converting the nonlinear function in the parameters into a set of linear algebraic equations. The nature of the linearized system and the factors influencing the accuracy of the parameter estimates have not yet been fully investigated. In this paper, LAM is applied to a nonlinear five-parameter model of the neuron. Ill-conditioning, which is often exhibited in LAM, is treated with the method of regularization as well as by the singular value decomposition (SVD). Simulation results indicate that the parameters estimated by LAM exhibit a remarkable robustness against additive white noise in comparison with the classical gradient optimization technique. Moreover, it is shown that regularization can be superior to SVD under certain conditions. Our results suggest that LAM can be used both as a noise reduction technique and as a stand-alone nonlinear parameter estimation algorithm. The comparison between LAM and a gradient technique show that, for this estimation problem, the LAM method can give more reliable estimates. Further improvements in estimation quality may still be achieved by the use of other forms of regularizing functions. PMID:8070806

Tawfik, B; Durand, D M

1994-05-01

457

Lymphatic Endothelial Differentiation in Pulmonary Lymphangioleiomyomatosis Cells  

PubMed Central

Pulmonary lymphangioleiomyomatosis (LAM) is a rare, low-grade neoplasm affecting almost exclusively women of childbearing age. LAM belongs to the family of perivascular epithelioid cell tumors, characterized by spindle and epithelioid cells with smooth muscle and melanocytic differentiation. LAM cells infiltrate the lungs, producing multiple, bilateral lesions rich in lymphatic channels and forming cysts, leading to respiratory insufficiency. Here we used antibodies against four lymphatic endothelial markers—podoplanin (detected by D2-40), prospero homeobox 1 (PROX1), vascular endothelial growth factor receptor 3 (VEGFR-3), and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1)—to determine whether LAM cells show lymphatic differentiation. Twelve of 12 diagnostic biopsy specimens (early-stage LAM) and 19 of 19 explants (late-stage LAM) showed immunopositivity for D2-40 in most neoplastic cells. PROX1, VEGFR-3, and LYVE1 immunoreactivity varied from scarce in the early stage to abundant in the late stage. Lymphatic endothelial, smooth muscle, and melanocytic markers were partially co-localized. These findings indicate that lymphatic endothelial differentiation is a feature of LAM and provide evidence of a previously unidentified third lineage of differentiation in this neoplasm. This study has implications for the histological diagnosis of LAM, the origin of the neoplastic cells, and potential future treatment with drugs targeting lymphangiogenesis. PMID:23609227

Davis, Jennifer M.; Hyjek, Elizabeth; Husain, Aliya N.; Shen, Le; Jones, Jennifer

2013-01-01

458

Application of scaleselective data assimilation to tropical cyclone track simulation  

E-print Network

zone" or interpolation when they are passed into the LAM. In this study, a dynamical downscaling in the LAM is thus determined by a dynamical equilibrium between the lateral boundary information and the internal model physics and dynamics [Giorgi, 2006]. However, this conventional nest- ingdown method

Liu, Paul

459

LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis  

PubMed Central

Mycobacterium tuberculosis employs various virulence strategies to subvert host immune responses in order to persist and cause disease. Interaction of M. tuberculosis with mannose receptor on macrophages via surface-exposed lipoarabinomannan (LAM) is believed to be critical for cell entry, inhibition of phagosome-lysosome fusion, and intracellular survival, but in vivo evidence is lacking. LprG, a cell envelope lipoprotein that is essential for virulence of M. tuberculosis, has been shown to bind to the acyl groups of lipoglycans but the role of LprG in LAM biosynthesis and localization remains unknown. Using an M. tuberculosis lprG mutant, we show that LprG is essential for normal surface expression of LAM and virulence of M. tuberculosis attributed to LAM. The lprG mutant had a normal quantity of LAM in the cell envelope, but its surface was altered and showed reduced expression of surface-exposed LAM. Functionally, the lprG mutant was defective for macrophage entry and inhibition of phagosome-lysosome fusion, was attenuated in macrophages, and was killed in the mouse lung with the onset of adaptive immunity. This study identifies the role of LprG in surface-exposed LAM expression and provides in vivo evidence for the essential role surface LAM plays in M. tuberculosis virulence. Findings have translational implications for therapy and vaccine development. PMID:25232742

Blumenthal, Antje; Bhamidi, Suresh; Gibbs, Sara; Jackson, Mary; Zare, Richard N.; Ehrt, Sabine; Ernst, Joel D.; Banaei, Niaz

2014-01-01

460

LprG-mediated surface expression of lipoarabinomannan is essential for virulence of Mycobacterium tuberculosis.  

PubMed

Mycobacterium tuberculosis employs various virulence strategies to subvert host immune responses in order to persist and cause disease. Interaction of M. tuberculosis with mannose receptor on macrophages via surface-exposed lipoarabinomannan (LAM) is believed to be critical for cell entry, inhibition of phagosome-lysosome fusion, and intracellular survival, but in vivo evidence is lacking. LprG, a cell envelope lipoprotein that is essential for virulence of M. tuberculosis, has been shown to bind to the acyl groups of lipoglycans but the role of LprG in LAM biosynthesis and localization remains unknown. Using an M. tuberculosis lprG mutant, we show that LprG is essential for normal surface expression of LAM and virulence of M. tuberculosis attributed to LAM. The lprG mutant had a normal quantity of LAM in the cell envelope, but its surface was altered and showed reduced expression of surface-exposed LAM. Functionally, the lprG mutant was defective for macrophage entry and inhibition of phagosome-lysosome fusion, was attenuated in macrophages, and was killed in the mouse lung with the onset of adaptive immunity. This study identifies the role of LprG in surface-exposed LAM expression and provides in vivo evidence for the essential role surface LAM plays in M. tuberculosis virulence. Findings have translational implications for therapy and vaccine development. PMID:25232742

Gaur, Rajiv L; Ren, Kangning; Blumenthal, Antje; Bhamidi, Suresh; Gibbs, Sara; Jackson, Mary; Zare, Richard N; Ehrt, Sabine; Ernst, Joel D; Banaei, Niaz

2014-09-01

461

Structural modifications of granular starch upon acylation with short-chain fatty acids  

Microsoft Academic Search

Low (LAMS) and high (HAMS) amylose maize starches acylated with acetic, propionic and butyric acids at three different degrees of substitution (DS) have been structurally characterized. SEM, XRD, SAXS and FT-IR were used to ascertain the effects of the molecular size of the esterified acid, the composition of the base maize starch and the DS on their structural properties. LAMS

Amparo Lopez-Rubio; Julie M. Clarke; Ben Scherer; David L. Topping; Elliot P. Gilbert

2009-01-01

462

Professional and Continuing Education in Hong Kong. Issues and Perspectives.  

ERIC Educational Resources Information Center

This book, which is based on several first-hand studies, provides an overview of professional and continuing education in Hong Kong and examines selected issues in the field. The following chapters are included: "Preface" (Lee Ngok, Agnes Lam); "Introduction" (Lee Ngok, Agnes Lam); "The Role of Government in Human Resource Development" (Lee Ngok,…

Ngok, Lee; Lam, Agnes

463

RICE UNIVERSITY Transport in Single Molecule Transistors  

E-print Network

RICE UNIVERSITY Transport in Single Molecule Transistors by Lam H. Yu A Thesis Submitted in Partial Houston, Texas January, 2006 #12;Abstract Transport in Single Molecule Transistors by Lam H. Yu molecule transistors (SMTs), nanometer-scale transistors in which charge transport occurs through

Natelson, Douglas

464

3, 30613097, 2006 Meteorological  

E-print Network

HESSD 3, 3061­3097, 2006 Meteorological analogues to account for LAM QPF uncertainty T. Diomede et System Sciences The use of meteorological analogues to account for LAM QPF uncertainty T. Diomede 1 , F. Nerozzi 1 , T. Paccagnella 1 , and E. Todini 2 1 Regional Hydro-Meteorological Service ARPA-SIM, Bologna

Paris-Sud XI, Université de

465

Where has the time gone? The role of time lags in models for longitudinal data  

E-print Network

of the existing solutions regarding the choice of lags for longitudinal models; 4) the introduction of an alternative strategy to addressing the lag issue: the lag as moderator (LAM) approach; and finally, 5) a demonstration of the potential of the LAM approach...

Selig, James Patrick

2009-01-01

466

Update on rescue therapies in patients with lamivudine-resistant chronic hepatitis B.  

PubMed

Chronic hepatitis B continues to be a global problem, with an estimated 240 million cases according to the World Health Organization. Chronic infection with the hepatitis B virus (HBV) is associated with cirrhosis, hepatic decompensation, and hepatocellular carcinoma. There are currently several US Food and Drug Administration-approved medications for treating chronic hepatitis B, with Lamivudine (LAM) being the first oral agent made available. The major problem with LAM is significantly decreased effectiveness over time due to the development of anti-HBV resistance that can lead to virologic and biochemical breakthrough as well as hepatitis B flare, progression of liver disease, and decompensation of pre-existing cirrhosis. Despite its high anti-HBV resistant rate, LAM remains widely used in underdeveloped countries due to its wide availability and low cost compared to other antiviral medications, including those that are more effective. Therefore, it is still clinically important to learn how to prevent and treat LAM resistant strains of HBV. Several regimens with the other available antiviral agents have been studied, including switching to monotherapy with either Adefovir, Entecavir, or Tenofovir, adding Adefovir to LAM, and switching to a combination of Adefovir and Entecavir. This review article will examine molecular mechanisms and diagnosis of LAM anti-HBV resistance, risks for and approaches to reduce LAM anti-HBV resistance, and currently available rescue therapy regimens for LAM resistance. PMID:23990707

Chao, Daniel C; Hu, Ke-Qin

2013-01-01

467

CT of Sclerotic Bone Lesions: Imaging Features Differentiating Tuberous Sclerosis Complex with Lymphangioleiomyomatosis from Sporadic Lymphangioleiomymatosis1  

PubMed Central

Purpose: To determine if sclerotic bone lesions evident at body computed tomography (CT) are of value as a diagnostic criterion of tuberous sclerosis complex (TSC) and in the differentiation of TSC with lymphangioleiomyomatosis (LAM) from sporadic LAM. Materials and Methods: Informed consent was signed by all patients in this HIPAA-compliant study approved by the institutional review board. Retrospective analysis was performed of the body CT studies of 472 patients: 365 with sporadic LAM, 82 with TSC/LAM, and 25 with TSC. The images were reviewed by using a picture archiving and communication system workstation with bone settings (window width, 1500 HU; window level, 300 HU) and fit-to-screen option. CT image characteristics assessed included shape, size, and distribution of sclerotic bone lesions with subsequent calculation of differences in the frequency of these lesions. Results: Most commonly the sclerotic bone lesions were round, measured 0.3 cm (range, 0.2–3.2), and were distributed throughout the spine. The frequencies differed among the three patient groups Four or more sclerotic bone lesions were detected in all 25 (100%) of those with TSC, with a sensitivity of .89 (72 of 82) and specificity of .97 (355 of 367) in the differentiation of sporadic LAM from TSC/LAM (P < .01). Conclusion: The number of sclerotic bone lesions at body CT is of potential value in the diagnosis of TSC and in the differentiation of patients with sporadic LAM from those with TSC/LAM. © RSNA, 2010 PMID:20177097

Dwyer, Andrew J.; Rabel, Antoinette; Darling, Thomas; Hong, Chien-Hui; Moss, Joel

2010-01-01

468

Lipoarabinomannan-Induced Cell Signaling Involves Ceramide and Mitogen-Activated Protein Kinase  

Microsoft Academic Search

Lipoarabinomannan (LAM) is a major cell wall-associated lipoglycan, produced in large amounts (15 mg\\/g of bacteria) in different species of mycobacteria. Our laboratory has previously reported that LAM from Mycobacterium smegmatis exerts its cytotoxic activity via inhibition of protein kinase C, a key signaling molecule inside the mononuclear cells (S. Ghosh, S. Pal, S. Das, S. K. Dasgupta, and S.

Madhumita Sirkar; Subrata Majumdar

2002-01-01