These are representative sample records from Science.gov related to your search topic.
For comprehensive and current results, perform a real-time search at Science.gov.
1

Infrared spectra in monitoring biochemical parameters of human blood  

NASA Astrophysics Data System (ADS)

Infrared spectroscopy is gaining recognition as a promising method. The infrared spectra of selected regions (2000-400cm-1) of blood tissue samples are reported. Present study related to the role of spectral peak fitting in the study of human blood and quantitative interpretations of infrared spectra based on chemometrics. The spectral variations are interpreted in terms of the biochemical and pathological processes involved. The mean RNA/DNA ratio of fitted intensities and analytical area as calculated from the transmittance peaks at 1121cm-1/1020cm-1 is found to be 0.911A.U and 2.00A.U. respectively. The ratio of 1659cm-1/1544cm-1 (amide-I/amide-II) bands is found to shed light on the change in the DNA content. The ratio of amide-I/amide-II is almost unity (?1.054) for blood spectra. The deviation from unity is an indication of DNA absorption from the RBC cells. The total phosphate content has found to be 25.09A.U. The level for glycogen/phosphate ratio (areas under peaks 1030cm-1/1082cm-1) is found to be 0.286A.U. The ratio of unsaturated and saturated carbonyl compounds (C=O) in blood samples is in form of esters and the analytical areas under the spectral peaks at 1740cm-1 and 1731cm-1 for unsaturated esters and saturated esters respectively found to be 0.618A.U.

Prabhakar, S.; Jain, N.; Singh, R. A.

2012-05-01

2

Correlation between liver weight and blood serum biochemical parameters in force fed interspecific hybrid ducks  

E-print Network

Correlation between liver weight and blood serum biochemical parameters in force fed interspecific are also found between large-liver and small-liver animals and with high weight gain or low gain. The following correlations are : liver size and GOT, r = + 0.583 ; liver size and GPT, r = + 0.490 ; liver size

Paris-Sud XI, Université de

3

Modulation of specific biochemical blood parameters by helminth infection in laboratory Beagle dogs.  

PubMed

The objective of this study was to examine the independent effect of helminths infection on biochemical blood parameters in Beagles intended for laboratory use which may contribute to a change of experimental results. As a result of research, the authors confirmed the negative effect of helminth invasion on the metabolism of the liver and kidney in laboratory dogs. Stool samples from thirty Beagle puppies were examined for parasites before the puppies were moved to the animal facility, and all were dewormed with Vetminth paste on the day they were moved. Stool examination was performed three more times and animals were given Drontal Plus flavor (Bayer) and Baycox 5% (Bayer). A fourth parasitological examination revealed no intestinal parasites in the feces. Three blood biochemical tests were performed. Experimental results clearly indicate the significant impact of intestinal parasites in dogs used in experiments. PMID:22844719

Szweda, M; Szarek, J; Babi?ska, I; Sokó?, R; Ra?-Nory?ska, M; Ko?odziejska-Sawerska, A; Mecik-Kronenberg, T

2012-01-01

4

The dynamics of blood biochemical parameters in cosmonauts during long-term space flights  

NASA Astrophysics Data System (ADS)

Most of the previously obtained data on cosmonauts' metabolic state concerned certain stages of the postflight period. In this connection, all conclusions, as to metabolism peculiarities during the space flight, were to a large extent probabilistic. The purpose of this work was study of metabolism characteristics in cosmonauts directly during long-term space flights. In the capillary blood samples taken from a finger, by "Reflotron IV" biochemical analyzer, "Boehringer Mannheim" GmbH, Germany, adapted to weightlessness environments, the activity of GOT, GPT, CK, gamma-GT, total and pancreatic amylase, as well as concentration of hemoglobin, glucose, total bilirubin, uric acid, urea, creatinine, total, HDL- and LDL cholesterol, triglycerides had been determined. HDL/LDL-cholesterol ratio also was computed. The crewmembers of 6 main missions to the "Mir" orbital station, a total of 17 cosmonauts, were examined. Biochemical tests were carryed out 30-60 days before lounch, and in the flights different stages between the 25-th and the 423-rd days of flights. In cosmonauts during space flight had been found tendency to increase, in compare with basal level, GOT, GPT, total amylase activity, glucose and total cholesterol concentration, and tendency to decrease of CK activity, hemoglobin, HDL-cholesterol concentration, and HDL/LDL — cholesterol ratio. Some definite trends in variations of other determined biochemical parameters had not been found. The same trends of mentioned biochemical parameters alterations observed in majority of tested cosmonauts, allows to suppose existence of connection between noted metabolic alterations with influence of space flight conditions upon cosmonaut's body. Variations of other studied blood biochemical parameters depends on, probably, pure individual causes.

Markin, Andrei; Strogonova, Lubov; Balashov, Oleg; Polyakov, Valery; Tigner, Timoty

5

The effects of copper on blood and biochemical parameters of rainbow trout (Oncorhynchus mykiss)  

USGS Publications Warehouse

Metals are released into aquatic systems from many sources, often at sublethal concentrations. The effects of sublethal concentrations of metals on fish are not entirely understood. The objective of this study was to determine the hematological and biochemical effects of a range of copper concentrations (6.4, 16.0, 26.9 ??g Cu/L) on rainbow trout (Oncorhynchus mykiss) over a prolonged period of time. Trout were exposed to copper, and, at intervals of 3, 7, 14, and 21 days, selected parameters were evaluated. Hemoglobin, hematocrit, plasma glucose, and plasma cortisol levels were elevated in trout exposed to 26.9 ??g Cu/L at day 3 and then returned to levels comparable to control fish. Plasma protein and lactate levels were not significantly altered in trout from any copper treatment. Hepatic copper concentration and hepatic metallothionein mRNA expression were consistently elevated in trout exposed to 26.9 ??g Cu/L. Both of these parameters stabilized by day 3, with only hepatic copper concentration showing a further increase at day 21. Hepatic copper concentration and hepatic metallothionein mRNA expression appear to be robust indicators of copper exposure. Most blood-based parameters evaluated appear to be associated with a transitory, nonspecific stress response. The return of elevated hematological and biochemical parameters to control levels after 3 days and thestabilization of hepatic metallothionein mRNA expression and copper concentration over a similar time period suggested acclimation to dissolved copper at 26.9 ??g/L. Further analysis of the data on blood-based parameters indicated that certain parameters (hemoglobin, hematocrit, plasma glucose, plasma cortisol) may be useful in field monitoring.

Dethloff, G.M.; Schlenk, D.; Khan, S.; Bailey, H.C.

1999-01-01

6

[Human blood biochemical parameters under the conditions of an arid zone].  

PubMed

Seven participants of 23 day walking march in an arid environment have been examined. Biochemical analysis of blood serum made on Day 2 of marching revealed a decrease in the levels of glucose, triglycerides and an increase in the concentration of non-esterified fatty acids which the authors characterize as the state of moderate stress in an acute period. PMID:1296835

Za?tseva, L B; Vetrova, E G; Delenian, N V; Popova, I A

1992-01-01

7

[Changes of biochemical blood parameters under conditions of treatment with vaulen and polysorb enterosorbents].  

PubMed

Alteration of the biochemical blood indices has been studied in patients with rheumatoid arthritis and lupus erythematosus that were treated with vaulen (V) and polysorb (P) enterosorbents. It was determined that alanine aminotransferase activity grew and albumin, urea, uric acid levels enhanced after the V treatment. Glucose level and alanine-, asparagine aminotransferases activities decreased after the P treatment. It is necessary to take into consideration these results when choosing an enterosorbent for treatment of patients with the immunocomplex rheumatic diseases accompanied by the liver parenchyma lesion and hyperuricemia. PMID:7754550

Iaremenko, O B; Ter-Vartan'ian, S Kh; Lopushenko, L G; Mikhal'chuk, H A

1994-01-01

8

Following the goose liver development by means of cross-sectional digital imaging, liver histology and blood biochemical parameters.  

PubMed

A follow-up study was performed to describe characteristic physiological alterations by means of computer tomography, direct chemical analysis and histology of the liver, and blood biochemical parameters during conventional force-feeding of Landes geese. 30 birds were exposed to an 18-day long force-feeding. Sampling was performed at the start and during force-feeding (7th, 11th, 14th, 18th days). Computer tomographic data were plotted in 3D histograms, effectively indicating the volumetric development and the fat deposition of the liver. Applying the so-called fat index, a saturation process was found for the hepatic fat content. Histological sections indicated the appearance of microvesicular fat forms in the hepatocyte cytoplasm, which first turned to a total fatty infiltration, later changing to a macrovesicular form with progressing inflammation; membrane damage was not visualized. In blood metabolites triglyceride, total and HDL cholesterol and uric acid increased measurably, while creatinine concentration decreased. Alanine aminotransferase, aspartate aminotransferase activities increased strongly, while that of lactate dehydrogenase only slightly. Based on the results of macroscopic and microscopic imaging techniques and blood biochemical parameters, a comprehensive follow-up study was performed, elucidating still unknown processes during force-feeding of geese. PMID:17385542

Locsmándi, L; Hegedüs, G; Andrássy-Baka, G; Bogenfürst, F; Romvári, R

2007-03-01

9

Grape Seed Proanthocyanidin Extract Improved some of Biochemical Parameters and Antioxidant Disturbances of Red Blood Cells in Diabetic Rats.  

PubMed

Grape seed proanthocyanidin extract (GSPE) has a broad spectrum of biologic properties against oxidative stress. This study aimed to investigate the effects of GSPE on biochemical factors and antioxidant enzymes of erythrocyte in diabetic rats. Diabetes was induced through single injection of streptozotocin (50 mg.Kg(-1), i.p). Forty Male Sprague-Dawley rats were divided into four Groups: Group 1, healthy control group; Group 2, healthy group treated with GSPE (200 mg.Kg(-1)); Group 3, diabetic control group; Group 4, diabetic group treated with GSPE (200 mg.Kg(-1)) for 4 weeks. At the end, the experimental animals were sacrificed and blood samples were collected and plasma parameters and erythrocytes antioxidant status were evaluated. The results show, treatment with GSPE significantly reduced (P<0.001) urine volume, proteinuria and biochemical factors such as blood urea nitrogen, creatinine, triglyceride, total cholesterol, low density lipoprotein and very low density lipoprotein as well as malondialdehyde. Also GSPE treatment significantly (P<0.005) increased high density lipoprotein, total protein and albumin. Moreover GSPE significantly increased antioxidant enzymes activity such as: superoxide dismutase, glutathione peroxidase and catalase. These results suggest that GSPE can ameliorate biochemical abnormalities and antioxidant system status in streptozotocin- induced diabetic rats probably by its potent antioxidant features. PMID:25561939

Mansouri, Esrafil; Khorsandi, Layasadat; Zare Moaiedi, Maasoumeh

2015-01-01

10

Grape Seed Proanthocyanidin Extract Improved some of Biochemical Parameters and Antioxidant Disturbances of Red Blood Cells in Diabetic Rats  

PubMed Central

Grape seed proanthocyanidin extract (GSPE) has a broad spectrum of biologic properties against oxidative stress. This study aimed to investigate the effects of GSPE on biochemical factors and antioxidant enzymes of erythrocyte in diabetic rats. Diabetes was induced through single injection of streptozotocin (50 mg.Kg-1, i.p). Forty Male Sprague-Dawley rats were divided into four Groups: Group 1, healthy control group; Group 2, healthy group treated with GSPE (200 mg.Kg-1); Group 3, diabetic control group; Group 4, diabetic group treated with GSPE (200 mg.Kg-1) for 4 weeks. At the end, the experimental animals were sacrificed and blood samples were collected and plasma parameters and erythrocytes antioxidant status were evaluated. The results show, treatment with GSPE significantly reduced (P<0.001) urine volume, proteinuria and biochemical factors such as blood urea nitrogen, creatinine, triglyceride, total cholesterol, low density lipoprotein and very low density lipoprotein as well as malondialdehyde. Also GSPE treatment significantly (P<0.005) increased high density lipoprotein, total protein and albumin. Moreover GSPE significantly increased antioxidant enzymes activity such as: superoxide dismutase, glutathione peroxidase and catalase. These results suggest that GSPE can ameliorate biochemical abnormalities and antioxidant system status in streptozotocin- induced diabetic rats probably by its potent antioxidant features. PMID:25561939

Mansouri, Esrafil; Khorsandi, Layasadat; Zare Moaiedi, Maasoumeh

2015-01-01

11

Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii)  

USGS Publications Warehouse

The effects of MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii) were studied. MS-222 caused higher glucose (GLU) concentrations in anaesthetic test groups than for the control group. Triglyceride (TGL) concentrations of fish in the 140 and 160mgL-1 groups were also significantly higher than those of other groups. Alanine aminotransferase (ALT) activity in the 140mgL-1 group was significantly higher than the level in 80, 100 and 120mgL-1 groups. Aspartate aminotransferase (AST) activity in the 140mgL-1 group was significantly higher than those in the 100 and 120mgL-1 groups. Levels of total protein (TP), cholesterol (CHOL) and alkaline phosphatase (ALP) in anaesthetic test groups were not significantly influenced by MS-222. Clove oil did not have significant effects on levels of GLU, TP, CHOL, ALT and ALP. TGL concentration of fish exposed to 180mgL-1 clove oil was significantly higher than those of the rest anaesthetic groups. AST activities of fish exposed to 120, 150 and 180mgL-1 were significantly higher than those of 60 and 90mgL-1. Overall, TGL and AST could be potentially used as indicators of anaesthetic stress for juvenile Siberian sturgeon. Based on blood biochemical parameters, the appropriate anaesthetic concentrations of MS-222 and clove oil were 80-120mgL-1 and 60-90mgL-1, respectively. Clove oil was a promising alternative to MS-222. ?? 2011 Blackwell Verlag, Berlin.

Feng, G.; Zhuang, P.; Zhang, L.; Kynard, B.; Shi, X.; Duan, M.; Liu, J.; Huang, X.

2011-01-01

12

[The influence of various technologies of sports massage on biochemical parameters of the blood].  

PubMed

Different variants of sports massage were tested on 8 fencers (masters and candidate masters of sports) aged between 18 and 22 years. Variant 1 was stimulating massage with the predominant impact on the selected groups of muscles while variant II included relaxation massage with the selective impact on the connective-tissue structures. Blood glucose, cortisol, thyroxin, triiodo-thyronine levels and concentrations of lactic, uric, and non-esterified fatty acids were measured before and after the massage. The results of the study indicate that different technological regimens of sports massage may cause multidirectional changes in the metabolism of selected biologically important compounds. The stimulating massage improves the functional state of the neuromuscular apparatus and activates energy production (in the first place, through enhanced utilization of the substances required for maintaining this process) which suggests formation of the mechanisms necessary for "emergency" adaptation. The relaxation massage suppresses the functional activity of the neuromuscular apparatus and energy consumption which results in the transition of the organism into the state dominated by accumulation of selected substances characteristic of "long-term" adaptation. PMID:22165148

Bykov, A T; Iakimenko, S N; Khodasevich, L S; Poliakova, A V

2011-01-01

13

Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions.  

PubMed

A study was conducted using 240 female day-old broiler chicks to evaluate the effects of dietary chromium picolinate (CrPic), peppermint essential oil (P.mint), or their combination on growth performance and blood biochemical parameters of female broiler chicks raised under heat stress conditions (HS, 23.9 to 38 °C cycling). Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were obtained from 1 to 42 days of age. Furthermore, at the end of the experiment (day 42), birds were bled to determine some blood biochemical parameters and weighed for final body weight (BW). ADFI, ADG, and BW were not influenced significantly by dietary CrPic and P.mint (P>0.05). A significant interaction between dietary CrPic and P.mint on FCR (P=0.012) was detected. FCR significantly decreased in chicks fed the diet including both CrPic and P.mint compared with the CrPic group. Significant interaction between dietary P.mint and CrPic on serum concentrations of triglycerides, glucose, and albumin were observed (P<0.05), but the other measured blood biochemical parameters were not statistically affected by dietary treatments (P>0.05). The serum concentrations of glucose, triglycerides were decreased (P<0.05) in broilers fed the diet including both CrPic and P.mint. Plasma chromium (Cr) content increased significantly (P<0.05) in birds fed the CrPic-included diet compared with the control group (P<0.05). From the results of the present experiment it can be concluded that dietary supplementation with combined P.mint and CrPic could have beneficial effects on some blood biochemical parameters of female chicks reared under heat stress conditions. PMID:24096939

Akbari, Mohsen; Torki, Mehran

2014-08-01

14

Impact of diets with vegetable oils on the growth, histological structure of internal organs, biochemical blood parameters, and proximate composition of pikeperch Sander lucioperca (L.)  

Microsoft Academic Search

The aim of the study was to evaluate the impact feed supplemented with vegetable oils had on the growth, histological structure of the liver, intestines, and spleen, selected biochemical blood parameters, and proximate body composition of juvenile pikeperch (initial body weight 33g). The fish were fed isoenergetic (21.0MJkg?1 feed) and isoprotein feeds (450g protein kg?1 feed) supplemented with fish and

Agata Kowalska; Zdzis?aw Zak??; Barbara Jankowska; Andrzej Siwicki

2010-01-01

15

Effect of Ensiled Mulberry Leaves and Sun-Dried Mulberry Fruit Pomace on Finishing Steer Growth Performance, Blood Biochemical Parameters, and Carcass Characteristics  

PubMed Central

Fifty-one Simmental crossbred steers (357.0±16.5 kg) were used to compare a standard total mix ration (TMR) with variants on animal performance, ruminal fermentation, blood biochemical parameters, and carcass characteristics. Corn grain and cotton seed meal were partially replaced by ensiled mulberry leaves (EML) or sun-dried mulberry fruit pomace (SMFP). Experimental diets had similar amounts of crude protein (CP), acid detergent fiber (ADF), and metabolizable energy (ME). Animals were divided into three groups: control group (CONT), 8% EML group, and 6.3% SMFP group. Performance, including average daily weight gain (ADG), and dry matter intake (DMI), was measured. Blood and rumen samples were collected at the end of the experiment (16 weeks). There were no differences in final body weight (P?=?0.743), ADG (P?=?0.425), DMI (P?=?0.642), or ADG/DMI (P?=?0.236) between the groups. There were no differences (P?=?0.2024) in rumen pH values; ammonia N was lower (P?=?0.0076) in SMFP than in the EML and CONT groups. There were differences in the concentrations of total and individual volatile fatty acids, while no differences were determined in blood biochemical parameters (i.e., plasma glucose, urea concentrations, triglycerides, total protein, insulin, IgG, alanine transaminase, and aspartate aminotransferase, P ? 0.098). No differences were observed in carcass characteristics (P ? 0.513), tenderness (P?=?0.844), adipose and lean color values (P ? 0.149), and chemical composition (P ? 0.400); however, intramuscular fat was lower in the EML and SMFP groups compared to the CONT animals (P?=?0.034). In conclusion, diets supplemented with these two mulberry products in an isocaloric and isonitrogenous manner have similar effects to corn grain and cotton seed meals on steer performance, blood biochemical parameters and carcass characteristics, with the exception of ruminal VFA concentrations and lower intramuscular fat content. PMID:24427304

Zhou, Zhenming; Zhou, Bo; Ren, Liping; Meng, Qingxiang

2014-01-01

16

Bas.J.Vet.Res.Vol.12,No.1,2013. THE EFFECT OF BISMUTH CHLORIDE ON SOME BLOOD AND BIOCHEMICAL PARAMETERS IN MALE LABORATORY RATS (RATTUS-RATTUS)  

E-print Network

The present study amid to characterized the potential toxic effects of bismuth chloride through oral administration on blood and biochiemical parameters of laboratory rats.Solutions of bismuth chloride were chronically feed by stomach tube to rats in(2.5mg/kg and 5 mg/kg).Animals were anesthetized after two months and blood samples were obtained,blood and biochemical parameters were appreciated between control and experimental Groups.The investigation of blood parameters included red blood cells count (RBC) hemoglobin concentration,packed cell volume PCV and total white blood cell count(WBC)biochemical parameters included total serum cholesterol(TSCH),alanin aminotransferase (ALT) and aspartate aminotransferase(AST).The results showed significant decrease (p?0.05) of R.B.C count,Hb concentrations PCV value and total W.B.C count in contrast,there was significant increase(p?0.05) of the total serum cholesterol(,ALTand AST) activities.

Zainab A. Shehab; Assad Hassan; Essa Adel; Mousa Hassan

17

Meat quality, some hematological and biochemical parameters in the blood of finishing pigs assigned to different carcass conformation classes in the SEUROP system.  

PubMed

The results of laboratory analyses, including the proximate composition, physicochemical and sensory properties of meat from the studied pigs, point to its high processing suitability and eating quality. Hematological indicators in the blood of the analyzed animal groups were within the reference ranges, excluding MCHC levels which were somewhat elevated. Significant differences were observed in Hb, HCT, MCV, MCH, MCHC and WBC values, subject to the meat content of the carcass. Biochemical parameters such as glucose concentrations, ALT and AST activity levels varied considerably between groups, and average ALT values somewhat exceeded the norm for the species. These results, including significant variations in selected parameters, can probably be attributed to homeostatic changes in pigs during intensive growth. PMID:24195297

Smieci?ska, K; Sobotka, W; Daszkiewicz, T; Hnatyk, N; Fiedorowicz, E

2013-01-01

18

The effect of mycotoxins found in some herbal plants on biochemical parameters in blood of female albino mice.  

PubMed

In this study, twenty five samples ofwell-known herbs in Riyadh, Saudi Arabia were collected and analyzed for Total Fungi Count (TFC). Mycotoxins were extracted and screened using SMKY liquid medium. One hundred and thirty adult female albino mice were grouped into three wherein one group (n = 110) was fed with an aqueous extract from herbal plants. The second group (n = 15) was fed with an aqueous extract of the isolated fungal species. The third group comprised the control group which was given water only (n = 5). All mice were fed with mice breeding diet by Pillsbury, UK. After 5 weeks, mice were fasted and blood was withdrawn for biochemical analysis including alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyltransferase (GGT), serum creatinine and urea. Calligonum comosum with 2 x 10(5) cfu g(-1) fungus spore, grained mixed herbs (24 x 10(3) cfu g(-1)) and Salvia officinalis (23 x 10(3) cfu g(-1)) were the most contaminated samples. The genus Aspergillus was the most dominant genus recovered (142 isolates) followed by Penicillium (14 isolates) and these two genera were found in 85.0 and 11.0% of the samples analyzed. Aspergillus fumigatus, Aspergillus flavus and Aspergillus ochraceus were the most dominant and frequently isolated (47.3, 46.5 and 18.1%, respectively), followed by Aspergillus citrinum (11.0%). Aspergillus ochraceus had 21.7 microg kg(-1) of Aflatoxin B2 and 7.25 microg kg(-1) of ochratoxin A, whereas Aspergillus flavus had 7.45 microg kg(-1) of Aflatoxin B2 and Aspergillus fumigatus had 3.5 microg kg(-1) of Aflatoxin B2 and 3.8 microg kg(-1) of ochratoxin A. Mean creatinine, urea, ALT, AST and GGT were higher in mice fed or treated with herbal and fungal extracts group than the control group. This study confirms previous studies demonstrating the predominance of Aspergillus species in herbal and medicinal plants and its capability in the production of aflatoxin with induction of nephrotoxicity and hepatoxicity in animals and even in humans. PMID:19634489

Alwakeel, Suaad S

2009-04-15

19

Effects of Azinphosmethyl on Some Biochemical Parameters in Blood, Muscle, and Liver Tissues of Cyprinus carpio(L.)  

Microsoft Academic Search

In the present study, the effects of 0.20 and 0.35 ppm azinphosmethyl exposures occurring on days 1, 2, 3, 4, and 15 for on serum enzyme activities and protein and glucose levels in blood, muscle, and liver tissues of fish,Cyprinus carpio(L.), were photometrically investigated. Protein and glycogen contents in muscle and liver tissues were also determined. Azinphosmethyl did not affect

Elif Özcan Oruç; Nevin Üner

1998-01-01

20

The effects of ACTH, prednisolone and Escherichia coli endotoxin on some clinical haematological and blood biochemical parameters in dwarf goats.  

PubMed

ACTH (microgram kg-1 i.v.) and prednisolone (1 microgram-1 i.v.) caused a moderate but statistically significant inhibition of rumen contractions, whereas no effects on heart rate and body temperature were observed. Both hormones induced hyperglycaemia and leucocytosis, characterised by moderate lymphopenia and a profound increase in the number of circulating neutrophils. A significant decrease in plasma iron and increase in plasma zinc concentrations were observed. After 3 daily i.m. injections of ACTH (10 micrograms-1 day-1) decreases were seen in both serum Alkaline phosphatase (ALP) activity and plasma trace metal concentrations; heart rate was significantly higher. Intraveneous injection of E. coli endotoxin (0.1 microgram kg-1) caused shivering, fever, inhibition of rumen contractions, changes in heart rate, lymphopenia, neutropenia followed by neutrophilic leucocytosis, hypoferraemia, hypozincaemia, hypoglycaemia and a decline in serum ALP activity. ACTH, given i.m. for 3 days, reduced the febrile responses to E. coli endotoxin, modified the changes in heart rate, intensified the inhibition of rumen contractions, and induced a more marked decrease in the number of circulating neutrophils. ACTH pretreatment did not affect the endotoxin-induced decrease in blood glucose concentrations nor the drop in plasma zinc and iron values. These results suggest that glucocorticosteroids are not primarily involved in the fall in plasma iron and zinc concentrations during E. coli endotoxin-induced fever, the effects of endotoxin released glucocorticosteroids on white blood cells and blood glucose are masked by some other effect(s) of endotoxin, and in dwarf goats, ACTH has antipyretic properties without influencing normal body temperature. This effect is probably not dependent on adrenal cortical activity. PMID:3018991

van Miert, A S; van Duin, C T; Wensing, T

1986-07-01

21

Effects of propolis on blood biochemical and hematological parameters in nitric oxide synthase inhibited rats by N?-Nitro-L-arginine methyl ester.  

PubMed

This study showed the effects of propolis on biochemical and hematological parameters in chronic nitric oxide synthase inhibited rats by N?-Nitro-L-arginine methyl ester (L-NAME). Rats are given L-NAME for 15 days and the propolis for the last 5 days with L-NAME together. The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and gamma glutamyltransferase in the L-NAME group compared to control group have increased (P<0.05). The levels of these parameters in L-NAME+propolis group compared to the L-NAME group have decreased (P<0.05). L-NAME caused increase (P<0.05) in levels of glucose, albumin, globulin, creatinine, urea, triglyceride and cholesterol. Erythrocyte number, total leukocyte, hemoglobin, hematocrit, neutrophil and monocyte decreased (P<0.05), platelets and lymphocyte increased (P<0.05) in L-NAME+propolis group compared to the L-NAME group. The study concluded that homeostasis is modulated in L-NAME administrated rats by adding propolis which causes increasing generation of vascular nitric oxide. PMID:24035946

Talas, Zeliha Selamoglu; Gogebakan, Ayse; Orun, Ibrahim

2013-09-01

22

Bioimpedance spectroscopy as technique of hematological and biochemical analysis of blood  

NASA Astrophysics Data System (ADS)

Bioimpedance spectroscopy may become a useful method for the express analysis and monitoring of blood parameters. The aim of this study was to identify biochemical and hematological parameters of blood that can be accurately predicted by means of bioimpedance technique. Hematological (red blood cell and white blood cell parameters) and biochemical (total proteins, albumins, fibrinogen, sodium, potassium, chloride ion concentrations in plasma) parameters were measured with a hematological analyzer and routine methods. Bioimpedance spectroscopy of the whole blood (1.5 ml) in frequency range 5-500 kHz (31 frequencies) was performed using BIA analyzer ABC-01 "Medass". Frequency relationships of resistance and reactance of the whole blood and the parameters of the Cole model were investigated. Close simple and multiple correlations of bioimpedance indices were observed only with erythrocyte parameters (Ht, Hb, RBC). Thus bioimpedance analysis of the whole blood can accurately predict red cell parameters but it is less effective for estimation of plasma biochemical and white cell parameters.

Malahov, M. V.; Smirnov, A. V.; Nikolaev, D. V.; Melnikov, A. A.; Vikulov, A. D.

2010-04-01

23

Effect of Quinoa Seeds ( Chenopodium quinoa ) in Diet on some Biochemical Parameters and Essential Elements in Blood of High Fructose-Fed Rats  

Microsoft Academic Search

The effect of Chenopodium quinoa seeds on lipid profile, glucose level, protein metabolism and selected essential elements (Na, K, Ca, Mg) level was determined\\u000a in high—fructose fed male Wistar rats. Fructose decreased significantly LDL [42%, p?p?p?blood of rats fed quinoa indicated, that these seeds effectively reduced serum total cholesterol\\u000a [26%, p?p?p?p?p?p?

Pawe? Pa?ko; Pawe? Zagrodzki; Henryk Barto?; Joanna Ch?opicka; Shela Gorinstein

2010-01-01

24

Biochemical blood markers and sampling sites in forensic autopsy.  

PubMed

Forensic pathologists often hesitate to use biochemical blood markers due to the risk of large postmortem changes and deviations from healthy subjects. Biochemical analyses of postmortem blood, if possible, may help to evaluate pathological status and determining the cause of death in forensic diagnosis, for example, in sudden unexpected death without obvious cause, or young adults with no apparent cause of death or antemortem information. Even commercially available biochemical markers were re-evaluated in the blood samples of 164 forensic autopsy cases. Biochemical markers examined were HbA1c, fructosamine, blood nitrogen urea (BUN), creatinine, total protein, total bilirubin, gamma-glutamyl transpeptidase (gamma-GTP), triglyceride, total cholesterol, C-reactive protein (CRP) and pseudocholine esterase (pChE). We collected cardiac blood (left cardiac blood and right cardiac blood) and peripheral blood (femoral vein blood) to clarify the differences in measured values by sampling site. The measured values were analyzed in relation to postmortem interval, etiology of death and sampling sites. Of all eleven markers, HbA1c is the most useful and reliable because of its negligible postmortem changes and small deviation from healthy subjects. Total bilirubin, BUN, CRP and total cholesterol can be useful if we set appropriate limit ranges and pay attention to the interpretation. For the evaluation of changes due to postmortem intervals, none of the markers except for triglyceride showed significant changes up to three days postmortem. As for sampling sites, femoral vein blood is generally recommended considering postmortem changes, but left cardiac blood was suitable for creatinine, pChE, and total cholesterol. For clinical forensic diagnosis of biochemical blood markers, we must determine the "forensic abnormal value" after collecting more cases by known causes with more information about the population. PMID:18511006

Uemura, Koichi; Shintani-Ishida, Kaori; Saka, Kanju; Nakajima, Makoto; Ikegaya, Hiroshi; Kikuchi, Yousuke; Yoshida, Ken-ichi

2008-07-01

25

Effect of quinoa seeds (Chenopodium quinoa) in diet on some biochemical parameters and essential elements in blood of high fructose-fed rats.  

PubMed

The effect of Chenopodium quinoa seeds on lipid profile, glucose level, protein metabolism and selected essential elements (Na, K, Ca, Mg) level was determined in high-fructose fed male Wistar rats. Fructose decreased significantly LDL [42%, p<0.01] and activity of alkaline phosphatase [20%, p<0.05], and increased triglycerides level [86%, p<0.01]. The analysis of blood of rats fed quinoa indicated, that these seeds effectively reduced serum total cholesterol [26%, p<0.05], LDL [57%, p<0.008] and triglycerides [11%, p<0.05] when compared to the control group. Quinoa seeds also significantly reduced the level of glucose [10%, p<0.01] and plasma total protein level [16%, p<0.001]. Fructose significantly decreased HDL [15%, p<0.05] level in control group but when the quinoa seeds were added into the diet the decrease of HDL level was inhibited. Quinoa seeds did not prevent any adverse effect of increasing triglyceride level caused by fructose. It was shown in this study that quinoa seeds can reduce most of the adverse effects exerted by fructose on lipid profile and glucose level. PMID:21104320

Pa?ko, Pawe?; Zagrodzki, Pawe?; Barto?, Henryk; Ch?opicka, Joanna; Gorinstein, Shela

2010-12-01

26

Comparison of biochemical parameters in pleural effusion.  

PubMed

A comparison of various biochemical parameters used in differentiation of nature of pleural effusion in transudate and exudate was done in 84 patient of pleural effusion. Pleural fluid (P) cholesterol and bilirubin which have gained an importance in recent years were compared with pleural fluid protein, LDH, P/S protein, P/S LDH and Light's criteria. It was seen that Light's criteria is still the best in differentiation. Sensitivity of individual test was nearly same for Pl. protein (94.11%), P/S Protein (94.11%), Pl. LDH (95.5%), P/S LDH (92.75%). Pl cholesterol (88.3%) and P/S cholesterol (91.42%) had slightly lower sensitivity. Pl. Protein and P/S LDH had 100% specificity. Lights criteria had 100% specificity and sensitivity % of cases misclassified by various criteria were Pl Protein 5.95%, P/S protein 5.95%, PLDH 4.76%, P/S LDH 4.76% P. Cholesterol 13% and P/S Cholesterol 9.52% Measurement of Bilirubin did not provide any correlation in classifying the effusion and thus did not hold any value. PMID:9251372

Lakhotia, M; Shah, P K; Yadav, A; Gupta, A; Modi, R K; Sinha, H V

1996-09-01

27

Diazinon toxicity affects histophysiological and biochemical parameters in rabbits.  

PubMed

Diazinon is a widely used pesticide in agriculture. So, the current work aimed to investigate the effects of diazinon exposure on some physiological and biochemical parameters, as well as, histopathological changes and histochemical acetyl-cholinesterase activity (AChE). The red Baladi rabbits were dipped into water (Control Group), diazinon at low concentrations of 0.6 mg diazinon low concentration (DLC) or high concentration of 3mg diazinon high concentration (DHC) dissolved in 1l of water for 10s. Treatment was repeated after 10 days and animals were sacrificed between 0 and 21 days after the second treatment. Blood analysis revealed that Red blood cells (RBC's), hemoglobin (Hb) and plasma total protein (TP) were significantly decreased in both diazinon concentrations (P<0.01), (P<0.05), (P<0.01) respectively. Cholesterol and microsomal protein were increased (P<0.01), while, liver/ body weight and cytochrome P-450 were decreased in both concentrations (P<0.01). Also there was a highly significant effect of concentration X day interaction on all parameters (P<0.01). Histopathological changes of liver, kidney and brain were observed after DHC dipping. Glycogen content was decreased in liver and increased in kidney Bowman's capsule. Furthermore, AChE activity was inhibited in brain tissue, decreased in liver cells, but gradually increased in kidney glomerular cells. Therefore, kidney and brain were highly affected by diazinon exposure compared with the liver. Exposure of animals to diazinon caused extensive changes in physiological, biochemical, and histopathological parameters as well as histochemical AChE. So, contact exposure of diazinon leads to negative response on animal health. PMID:17933502

Yehia, Mona A H; El-Banna, Sabah G; Okab, Aly B

2007-11-01

28

Blood biochemical and cellular changes during decompression and simulated extravehicular activity  

NASA Technical Reports Server (NTRS)

Blood biochemical and cellular parameters were measured in human subjects before and after exposure to a decompression schedule involving 6 h of oxygen prebreathing. The exposure was designed to simulate extravehicular activity for 6 h (subjects performed exercise while exposed to 29.6 kPa). There were no significant differences between blood samples from subjects who were susceptible (n = 11) versus those who were resistant (n = 27) to formation of venous gas emboli. Although several statistically significant (P less than 0.05) changes in blood parameters were observed following the exposure (increases in white blood cell count, prothrombin time, and total bilirubin, and decreases in triglycerides, very-low-density lipoprotein cholesterol, and blood urea nitrogen), the changes were small in magnitude and blood factor levels remained within normal clinical ranges. Thus, the decompression schedule used in this study is not likely to result in blood changes that would pose a threat to astronauts during extravehicular activity.

Jauchem, J. R.; Waligora, J. M.; Johnson, P. C. Jr

1990-01-01

29

The rat adequately reflects human responses to exercise in blood biochemical profile: a comparative study.  

PubMed

Animal models are widely used in biology and the findings of animal research are traditionally projected to humans. However, recent publications have raised concerns with regard to what extent animals and humans respond similar to physiological stimuli. Original data on direct in vivo comparison between animals and humans are scarce and no study has addressed this issue after exercise. We aimed to compare side by side in the same experimental setup rat and human responses to an acute exercise bout of matched intensity and duration. Rats and humans ran on a treadmill at 86% of maximal velocity until exhaustion. Pre and post exercise we measured 30 blood chemistry parameters, which evaluate iron status, lipid profile, glucose regulation, protein metabolism, liver, and renal function. ANOVA indicated that almost all biochemical parameters followed a similar alteration pattern post exercise in rats and humans. In fact, there were only 2/30 significant species × exercise interactions (in testosterone and globulins), indicating different responses to exercise between rats and humans. On the contrary, the main effect of exercise was significant in 15/30 parameters and marginally nonsignificant in other two parameters (copper, P = 0.060 and apolipoprotein B, P = 0.058). Our major finding is that the rat adequately mimics human responses to exercise in those basic blood biochemical parameters reported here. The physiological resemblance of rat and human blood responses after exercise to exhaustion on a treadmill indicates that the use of blood chemistry in rats for exercise physiology research is justified. PMID:25677548

Goutianos, Georgios; Tzioura, Aikaterini; Kyparos, Antonios; Paschalis, Vassilis; Margaritelis, Nikos V; Veskoukis, Aristidis S; Zafeiridis, Andreas; Dipla, Konstantina; Nikolaidis, Michalis G; Vrabas, Ioannis S

2015-02-01

30

Biochemical Parameters of Orienteers Competing in a Long Distance Race.  

ERIC Educational Resources Information Center

Measured important biochemical parameters in a group of orienteers two hours before beginning and immediately after an orienteering marathon. Found levels of dehydration. Suggests a drinking regimen which is designed for orienteering races. Concludes that no runner having kidney or liver abnormalities or changes in the urine should be allowed to…

Mikan, Vladimir; And Others

1992-01-01

31

[Reference values of Russian cosmonauts blood biochemical indices].  

PubMed

Within the period of 2000 to 2012 the values of 40 diagnostically significant biochemical indices in venous blood were being determined during the pre-flight clinical and physiological examination of 28 Russian cosmonauts aged 35 to 54, members of main and back-up crews of expeditions to the International Space Station. The above examination was conducted 45-30 days prior to the launch. Taking account of the fact that each of the most of the cosmonauts performed several flights over the mentioned period and they were repeatedly included in back-up crews over and over again, each of cosmonauts participated in pre-flight examinations 1 to 5 times. Reference values were calculated for each of the studied indices. It was found that reference interval boundaries for 15 indices were narrower as compared to the generally accepted ranges. The upper boundaries of activity reference ranges of a number of enzymes, as well as of concentration of some metabolites of energy and plasticity metabolism were raised relative to mean population values. Thus, it was found that Russian cosmonauts being a specific professional team featured inherent reference values which differed from mean population values in a number of blood biochemical indices. It is conditioned by peculiarities of selection, physical training and psychic and emotional status of crew members during a pre-flight period. PMID:23789387

Markin, A A; Zhuravleva, O A; Morukov, B V; Kuzichkin, D S; Zabolotskaia, I V; Vostrikova, L V

2013-01-01

32

Temporal sequence of major biochemical events during Blood Bank storage of packed red blood cells  

PubMed Central

Background. We used sensitive spectroscopic techniques to measure changes in Band 3 oligomeric state during storage of packed red blood cells (RBC); these changes were compared to metabolic changes, RBC morphology, cholesterol and membrane protein loss, phospholipid reorganisation of the RBC membrane, and peroxidation of membrane lipid. The aim of the study was to temporally sequence major biochemical events occurring during cold storage, in order to determine which changes may underlie the structural defects in stored RBC. Materials and methods. Fifteen RBC units were collected from normal volunteers and stored under standard blood bank conditions; both metabolic changes and lipid parameters were measured by multiple novel assays including a new mass spectrometric measurement of isoprostane (lipid peroxidation) and flow cytometric assessment of CD47 expression. Band 3 oligomeric state was assessed by time-resolved phosphorescence anisotropy, and RBC morphology by microscopy of glutaraldehyde-fixed RBC. Results. Extracellular pH decreased and extracellular potassium increased rapidly during cold storage. Band 3 on the RBC membrane aggregated into large oligomers early in the storage period and coincident with changes in RBC morphology. Membrane lipid changes, including loss of unesterified cholesterol, lipid peroxidation and expression of CD47, also changed early during the storage period. In contrast loss of acetylcholinesterase activity and haemolysis of RBC occurred late during storage. Discussion. Our results demonstrate that changes in the macromolecular organisation of membrane proteins on the RBC occur early in storage and suggest that lipid peroxidation and/or oxidative damage to the membrane are responsible for irreversible morphological changes and loss of function during red cell storage. PMID:22507860

Karon, Brad S.; van Buskirk, Camille M.; Jaben, Elizabeth A.; Hoyer, James D.; Thomas, David D.

2012-01-01

33

Similar hematological and biochemical parameters among periodontitis and control group subjects  

PubMed Central

Objective There are conflicting reports in the past literature documenting the tendency of anemia in patients with periodontitis. Hence, this study was undertaken to assess whether periodontitis may cause an anemic state, by evaluating and comparing the red blood cell count, levels of hemoglobin, hematocrit, erythrocyte sedimentation rate (ESR), serum iron and serum ferritin between subjects with and without periodontitis. Methods: In this cross-sectional study, 140 systemically healthy subjects of both sexes (mean age 46 years) were recruited as control group (50 subjects without periodontitis) and study groups comprising 30 patients each with mild, moderate and severe chronic generalized periodontitis. Periodontal parameters and orthopantamographs were taken for all the groups and then 5 mL venous blood samples were sent for complete blood count and biochemical analysis. Inter-group and intra-group comparisons were performed for all the assessed parameters. Results: The periodontal parameters were significantly higher (P?.05) in periodontitis patients. Except for the ESR, which was significantly higher (P=.03) in the mild periodontitis group than the control group, hematological and biochemical parameters were not significantly different (P>.05) among the study groups or between the control and study groups. This difference was not evident even among the male and female subjects of both control and study groups (P>.05). Conclusions: Within the limits of this cross-sectional study, it can be concluded that the presence and severity of periodontitis may not affect the hematological and biochemical parameters of an individual. Further long term studies are however encouraged to validate these findings. PMID:22904657

Prakash, Shobha; Dhingra, Kunaal; Priya, Shanmuga

2012-01-01

34

Effects of various propolis concentrations on biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss).  

PubMed

Biochemical and hematological parameters in blood of rainbow trout treated to various concentrations of propolis for 96 h were determined. Total leukocyte count and granulocytes values increased (p<0.05) in 0.02 and 0.03 g/L propolis groups. There was a decrease in agranulocytes (p<0.05) erythrocytes, hemoglobin and hematocrit values for fish exposed to 0.02 and 0.03 g/L propolis. MCV and MCH values (p<0.05) were significantly increased; 0.02 and 0.03 g/L propolis caused an increase (p<0.05) in the levels of glucose, blood urea nitrogen, triglyceride, total cholesterol, lactate dehydrogenase, amylase and gamma glutamyltransferase. There was a decrease in the levels of aspartate aminotransferase and alkaline phosphatase. Hematological and biochemical protective effects of 0.01 g/L propolis were investigated. Dose-dependent effects of propolis on blood of fish can be favorable, opening new perspectives of investigation on their biological properties and utilization. PMID:19428108

Talas, Zeliha Selamoglu; Gulhan, Mehmet Fuat

2009-10-01

35

Modulation of DMBA-induced biochemical changes by organoselenium compounds in blood of rats.  

PubMed

The protective role of two synthetic organoselenium compounds 1-isopropyl-3-methylbenzimidazole-2-selenone (SeI) and 1, 3-di-p-methoxybenzylpyrimidine-2-selenone (Sell) was examined against the 7,12-dimethylbenz[a]anthracene (DMBA)-induced changes in biochemical parameters in blood of rats. Albino Winstar rats (150-200 g body wt) were treated with single dose of DMBA (50 mg/kg body wt) and organoselenium compounds (25 micromol/kg) for 4 weeks at two days internal. Blood was taken from the anaesthetized rats ventricle from their hearts for biochemical analysis. Administration of DMBA resulted in elevation of urea, uric acid and creatinine levels as well as AST, ALT and LDH activities and decrease in levels of total proteins, albumin and globulin. SeI and SeII caused a significant (p<0.05) decrease in urea, uric acid and creatinine levels and alanine aminotransferase (ALT); aspartate aminotransferase; (AST) and lactate dehydrogenase (LDH) activities and significantly increased the levels of total protein and albumin (p<0.05). These organoselenium compounds are likely to be beneficial in human health. PMID:17970285

Ozdemir, I; Selamoglu, Z; Ates, B; Gok, Y; Yilmaz, I

2007-08-01

36

Serum Biochemical and Hematological Parameters of Sinclair(S-1) Miniature Sows During Gestation and Lactation  

PubMed Central

Venous blood samples were collected from 29 Sinclair(S-1) miniature sows at 14, ten, six and two weeks prior to parturition and two, four and six weeks postpartum to determine the effect of pregnancy and lactation upon 19 serum biochemical and 12 hematological parameters. During gestation, the levels of serum cholesterol, blood urea nitrogen and alpha1-globulin, as well as packed cell volume and hemoglobin concentration, decreased; whereas, the level of serum beta-globulin increased. During lactation, the concentrations of serum glucose, total protein, albumin, beta-globulin, calcium, sodium and hemoglobin, as well as packed cell volume, decreased; whereas, the concentration of serum cholesterol and the activity of serum alkaline phosphatase increased. PMID:4249094

Tumbleson, M. E.; Burks, Marlyn F.; Spate, Marilyn P.; Hutcheson, D. P.; Middleton, C. C.

1970-01-01

37

[Biochemical plasma parameters in rats during intravenous injection of magnetite nanopowder].  

PubMed

Nanotechnology is now recognized as a priority area of researches. Despite its rapid development, the literature contains a paucity of evidence for the effect of nanoparticles on the biochemical plasma parameters of experimental animals. This paper determines the biochemical parameters reflecting the functional state of the rat vital organs. Intravenous administration of a standardized Fe3O4 nanopowder solution has shown reversible changes in the biochemical parameter of plasma. PMID:19198256

Mil'to, I V; Kalugina, E F; Magaeva, A A

2008-01-01

38

Blood Storage Duration and Biochemical Recurrence of Cancer After Radical Prostatectomy  

PubMed Central

OBJECTIVE: To test the hypothesis that perioperative transfusion of allogeneic and autologous red blood cells (RBCs) stored for a prolonged period speeds biochemical recurrence of prostate cancer after prostatectomy. PATIENTS AND METHODS: We evaluated biochemical prostate cancer recurrence in men who had undergone radical prostatectomy and perioperative blood transfusions from July 6, 1998, through December 27, 2007. Those who received allogeneic blood transfusions were assigned to nonoverlapping “younger,” “middle,” and “older” RBC storage duration groups. Those who received autologous RBC transfusions were analyzed using the maximum storage duration as the primary exposure. We evaluated the association between RBC storage duration and biochemical recurrence using multivariable Cox proportional hazards regression. RESULTS: A total of 405 patients received allogeneic transfusions. At 5 years, the biochemical recurrence–free survival rate was 74%, 71%, and 76% for patients who received younger, middle, and older RBCs, respectively; our Cox model indicated no significant differences in biochemical recurrence rates between the groups (P=.82; Wald test). Among patients who received autologous transfusions (n=350), maximum RBC age was not significantly associated with biochemical cancer recurrence (P=.95). At 5 years, the biochemical recurrence–free survival rate was 85% and 81% for patients who received younger and older than 21-day-old RBCs, respectively. CONCLUSION: In patients undergoing radical prostatectomy who require RBC transfusion, recurrence risk does not appear to be independently associated with blood storage duration. PMID:21282486

Cata, Juan P.; Klein, Eric A.; Hoeltge, Gerald A.; Dalton, Jarrod E.; Mascha, Edward; O'Hara, Jerome; Russell, Amanda; Kurz, Andrea; Ben-Elihayhu, Shamgar; Sessler, Daniel I.

2011-01-01

39

Investigation of hematological and biochemical parameters in small ruminants naturally infected with Babesia ovis  

PubMed Central

Babesia ovis plays an important role in causing anemia and kidney dysfunction in affected animals. There are few extensive studies about hematological and biochemical findings of small ruminants' babesiosis caused by B. ovis. The aim of this study was to evaluate the effect of babesiosis on some hematological and biochemical parameters in infected small ruminants with B. ovis. A total of 280 sheep and 122 goats from 40 herds were randomly examined for the presence of B. ovis in blood samples. Of 402 samples, 67 animals (16.7%) were positive for B. ovis of which 52 (18.5%) were sheep and 15 (12.2%) goats, respectively. The infected animals were divided into four subgroups according to parasitemia rates (<1%, 1%, 2%, and 3%). As a control group, 67 uninfected animals were also selected from the same farms. With increase in parasitemia rates, hemoglobin concentration (Hb), packed cell volume (PCV), red blood cells (RBCs), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) significantly decreased (P < 0.05), while, total leukocyte count, number of lymphocyte, monocyte, neutrophil and eosinophil showed a significant increase (P < 0.05). Infected animals presented a significant elevation (P < 0.05) of total proteins and significantly lower level (P < 0.05) of albumin compared to non-infected animals. Significant elevation (P < 0.05) of BUN, creatinine, cholestrol, triglyceride, HDL and LDL level were found with parasitemia progression.

Esmaeilnejad, Bijan; Tavassoli, Mousa; Asri-Rezaei, Siamak

2012-01-01

40

Berberis Fruit Extract and Biochemical Parameters in Patients With Type II Diabetes  

PubMed Central

Background: Diabetes mellitus is a common medical problem. There is in fact a growing body of literature on plants used for the treatment of diabetes. Plant materials attracted considerable interest of scientists. In this respect, in the past few years, attempts were made to use natural plant products for the treatment of patients with diabetes. Objectives: The aim of this study was to investigate the effect of Berberis fruit extract to achieve glycemic control in patients with Type II diabetes. Materials and Methods: This study was performed between July 2010 and April 2013. Thirty patients of type II diabetes admitted to Ayatollah Rohhani hospital were recruited. Patients’ sera were collected for the assessment of glucose and HbA1c values. Biochemical analyses were performed before and after treatment by Berberis fruit extract. Biochemical parameters were measured by spectrophotometric method (Jenway uv/vis, 6505 model, Dunmow, UK). Glucose level was measured by glucose oxidase method kit (Pars Azmoon, Tehran, IR Iran). Serum total cholesterol and triglycerides were measured using standard biochemical kits (Pars Azmoon, Tehran, Iran). Blood glycated hemoglobin level was measured by using Elisa kit (Bioassay technology laboratory, Elisa kit). Experiments were performed in triplicate in at least three separate experiments. Results: Our findings demonstrated that patients with type 2 diabetes who received barberry fruit had significant reduction in serum glucose to 136.15 ± 32.8 mg/dL and decreased HbA1c levels to 7.07 ± 1.21 mg/dL, during the 8 weeks of study. Conclusions: This investigation revealed that Berberis fruit extract has beneficial metabolic effects in patients with type II diabetes. Barberry may improve glucose catabolism via glycolysis pathway, stimulate insulin secretion or improve insulin function and finally decrease glucose uptake. Our results indicated that Berberis fruit regulates glucose metabolism in patients with type 2 diabetes. PMID:24872938

Moazezi, Zolikha; Qujeq, Durdi

2014-01-01

41

VARIATION OF SOME BLOOD BIOCHEMICAL CHARACTERISTICS IN CATTLE, HORSES AND DOGS,  

E-print Network

VARIATION OF SOME BLOOD BIOCHEMICAL CHARACTERISTICS IN CATTLE, HORSES AND DOGS, AND CAUSES cattle. A few examples will also be given concerning horses and dogs. NORMAL RANGE In biology the concept the normal ranges for dairy cattle (Swedish Red and White Breed), horses (standardbred trotters) and dogs

Paris-Sud XI, Université de

42

Propagation of Blood Clotting in the Complex Biochemical Network of Hemostasis Is Described by a Simple Mechanism  

E-print Network

Propagation of Blood Clotting in the Complex Biochemical Network of Hemostasis Is Described propagation of blood clotting from one vessel to another. The network of hemostasis controls blood clotting and localize blood clotting at sites of vascular injury. When this network fails to function correctly

Ismagilov, Rustem F.

43

The clinical utility of screening of biochemical parameters in elite athletes: analysis of 100 cases  

Microsoft Academic Search

Objective:To determine the clinical utility of screening for biochemical parameters in elite athletes.Design:A prospective sequential case series.Setting:The Department of Sports Medicine at the Australian Institute of Sport.Participants:100 elite athletes from 11 sports (56 male and 44 female athletes, mean age 19 years, range 16–27), undergoing routine medical screening.Intervention:Initial and follow-up assessment of the following biochemical parameters in association with clinical

K E Fallon

2008-01-01

44

Cadmium chloride toxicity revisited: effect on certain andrological, endocrinological and biochemical parameters of adult male rabbits.  

PubMed

The present study was devised to assess the effects of cadmium chloride (CdCl(2)) administration on certain andrological, endocrinological and biochemical alterations in adult male rabbits (n=24). The animals were assigned to control (n=8) and experimental (n=16) group. Experimental group was orally administered with 1.5 mg/kg body weight of CdCl(2). The trials were carried out for a total of 5 weeks and blood sampling was carried out on weekly basis. A gradual decrease was noticed for body weight in the experimental group from week 1 to 5, being significantly lower in week 4 and 5 (P<0.05). A similar decremented trend was noticed for serum testosterone level being significantly lower in experimental group in week 4 and 5 (P<0.001). Significantly lower values were noticed for prolactin in experimental group in week 4 and 5 (P<0.05), than in the control. On the contrary, serum cortisol level showed a gradual increase in experimental group, from week 1 to 5, being significantly higher in week 4 and 5 (P<0.05). Regarding the biochemical attributes, all the parameters under study revealed a gradually ascending trend. Statistical significance was, however, achieved in varying weeks and at varying levels. The total protein and albumin were significantly higher in week 4 and 5 (P<0.01); alanine aminotransferase in week 2 (P<0.01), 3 (P<0.001), 4 (P<0.01) and 5 (P<0.001); aspartate aminotransferase in week 1, 2, 3, 4 and 5 (P<0.01); and alkaline phosphatase in week 1, 2 (P<0.01), 3, 4 and 5 (P<0.0001), respectively. Overall mortality rate in experimental group was 68.75 (11/16). In a nutshell, Cd exposure results in adverse effects on all physiological parameters of body and may lead to lethal consequences. PMID:24702494

Sajjad, S; Malik, H; Farooq, U; Rashid, F; Nasim, H; Tariq, S; Rehman, S

2014-09-01

45

Changes of hepatic biochemical parameters and proteomics in broilers with cold-induced ascites  

PubMed Central

Ascites syndrome is still a problem for chicken industry in various parts of the world. Despite the intensive investigations of this syndrome for many years, its pathogenesis remains unclear. The objective of this study was to analyze the difference in hepatic proteomics between ascites and healthy broilers by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Changes of biochemical parameters of liver and blood were also determined. The results indicated that red blood cell counts (RBC), hematocrit (HCT) and haemoglobin (HGB) of ascites broilers were significantly greater than healthy broilers. Hepatic malondialdehyde (MDA) level of ascites broilers was significantly increased, and the activity of total superoxide dismutase (T-SOD) was significantly decreased. Hepatic lactic acid (LD) level of ascitic broilers were significantly lower than healthy ones. Serum glucose and cholesterol level of ascites broilers were significantly increased, and serum globulin level was significantly decreased in ascites broilers. There was no significant difference in triglyceride (TG) and blood urea nitrogen (BUN) level. The activity of liver hexokinase (HK) and succinodehydrogenase (SDH) in ascites broilers was significantly decreased, and there was no significant difference in the activity of liver pyruvate kinase (PK) and Na+-K+-ATPase. The hepatic proteomics analysis showed that 18 proteins expression difference were identified between ascites and healthy broilers. These proteins were mainly involved in: 1) cytoskeleton; 2) glucose, lipids and amino acid metabolism; 3) cell secretion; 4) cell apoptosis; 5) signal transduction; 6) immune and inflammatory response; and 7) cellular redox homeostasis. Mitochondrial isoform phosphoenolpyruvate carboxykinase (M-PEPCK) mainly participates in gluconeogenesis of chicken liver. In conclusion, liver oxidative damage was significantly aggravated, but antioxidant capacity was decreased in cold-induced ascites broilers. Serum glucose level was significantly increased, with liver M-PEPCK expression higher in ascites broilers, which implied that some potential regulatory reagents may reduce ascites susceptibility and mortality under cold temperature by increasing liver gluconeogenesis level. PMID:23232037

2012-01-01

46

Changes of hepatic biochemical parameters and proteomics in broilers with cold-induced ascites.  

PubMed

Ascites syndrome is still a problem for chicken industry in various parts of the world. Despite the intensive investigations of this syndrome for many years, its pathogenesis remains unclear. The objective of this study was to analyze the difference in hepatic proteomics between ascites and healthy broilers by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Changes of biochemical parameters of liver and blood were also determined. The results indicated that red blood cell counts (RBC), hematocrit (HCT) and haemoglobin (HGB) of ascites broilers were significantly greater than healthy broilers. Hepatic malondialdehyde (MDA) level of ascites broilers was significantly increased, and the activity of total superoxide dismutase (T-SOD) was significantly decreased. Hepatic lactic acid (LD) level of ascitic broilers were significantly lower than healthy ones. Serum glucose and cholesterol level of ascites broilers were significantly increased, and serum globulin level was significantly decreased in ascites broilers. There was no significant difference in triglyceride (TG) and blood urea nitrogen (BUN) level. The activity of liver hexokinase (HK) and succinodehydrogenase (SDH) in ascites broilers was significantly decreased, and there was no significant difference in the activity of liver pyruvate kinase (PK) and Na+-K+-ATPase. The hepatic proteomics analysis showed that 18 proteins expression difference were identified between ascites and healthy broilers. These proteins were mainly involved in: 1) cytoskeleton; 2) glucose, lipids and amino acid metabolism; 3) cell secretion; 4) cell apoptosis; 5) signal transduction; 6) immune and inflammatory response; and 7) cellular redox homeostasis. Mitochondrial isoform phosphoenolpyruvate carboxykinase (M-PEPCK) mainly participates in gluconeogenesis of chicken liver. In conclusion, liver oxidative damage was significantly aggravated, but antioxidant capacity was decreased in cold-induced ascites broilers. Serum glucose level was significantly increased, with liver M-PEPCK expression higher in ascites broilers, which implied that some potential regulatory reagents may reduce ascites susceptibility and mortality under cold temperature by increasing liver gluconeogenesis level. PMID:23232037

Wang, Yongwei; Guo, Yuming; Ning, Dong; Peng, Yunzhi; Cai, Hong; Tan, Jianzhuang; Yang, Ying; Liu, Dan

2012-01-01

47

[Effect of hypoxia on biochemical parameters of Scorpaena erythrocytes].  

PubMed

Hypoxia effect on the nuclear of the Scorpaena porcus (L.) in vivo was studied. It was shown, that existence of the fishes in environmental with low oxygen concentration-1.3-1.4 mg.1-1 (15% initial saturation) resulted in reducing in activities of Na+, K(+)-ATPase, hexokinase and glucose-6-phosphate dehydrogenase in the erythrocyte for 50.0 (p < 0.001), 26.5% (p < 0.01) and 53.7% (p < 0.05) accordingly. ATP concentration in cells and membrane gradient of Na+, K+ concentrations between blood serum and intracellular environment did not change. A conclusion was made about a decrease of cells membrane penetration and oppression of intracellular metabolism. These changes proceeded on a background of the blood serum dehydration and decrease of the mean volume of erythrocytes. The part of aldosteron and vasopressin in membrane penetration of nuclear erythrocytes is discussed. PMID:7747338

Soldatov, A A; Rusinova, O S; Trusevich, V V; Zvesdina, T F

1994-01-01

48

Contaminant concentrations, biochemical and hematological biomarkers in blood of West Indian manatees Trichechus manatus from Brazil.  

PubMed

The West Indian manatee Trichechus manatus is threatened with extinction in Brazil, and this study focused on nondestructive blood samples analyzed for metals, polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), as well as biochemical and hematological biomarkers. Studied manatees were kept at Projeto Peixe-Boi headquarters in Pernambuco State, and at two natural areas in estuaries where they are released to the wild. Manatees kept at the natural estuary in Paraiba State have blood concentrations of Al, Pb, Cd, Sn that are 11, 7, 8 and 23 times greater, respectively, than the concentrations found in blood of animals from the same species in Florida, USA. An inhibition of butyrylcholinesterase in manatees kept at the two reintroduction sites in Alagoas and Paraiba States indicated possible exposure of the animals to cholinesterase inhibitor insecticides. PCBs and OCPs were not detected. Results from this study will help delineate conservation efforts in the region. PMID:22626623

Anzolin, D G; Sarkis, J E S; Diaz, E; Soares, D G; Serrano, I L; Borges, J C G; Souto, A S; Taniguchi, S; Montone, R C; Bainy, A C D; Carvalho, P S M

2012-07-01

49

Pattern recognition methods in evaluation of the structure of the laboratory data biominerals, antioxidant enzymes, selected biochemical parameters, and pulmonary function of welders.  

PubMed

A wide set of biochemical and spirometrical parameters was applied to a group of 41 welders. The obtained parameters were analyzed by the pattern recognition method: principal-components analysis (PCA) and mutual contribution analysis (MCA). The aim of the investigation was to find out which of 23 parameters had an influence on the pulmonological indices of COPD. It was found that PCA gave a substantial reduction of dimensionality. The most relevant parameters were Fe, Zn, Cu (all elements in blood, not in hair), FEV1, MEF50, bilirubin, TAS, and SOD. Only the relationships of MEF50, Cu, and TAS were statistically valid. PMID:12835488

Stepniewski, Marek; Kolarzyk, Emilia; Zagrodzki, Pawel; Solarska, Katarzyna; Pietrzycka, Agata; Kitlinski, Mariusz; Schlegel-Zawadzka, Malgorzata; Batko, Bogdan

2003-01-01

50

Biochemical parameters as prognostic factors in prostatic adenocarcinoma.  

PubMed

The serum levels of creatinine (CR), alkaline phosphatase (ALP), acid phosphatase (ACP) and tartrate inhibitable acid phosphatase (TIAP) were related to Gleason score, TM-category, disease progression and survival in 325 prostatic adenocarcinoma patients followed up for over 12 years. Elevated serum levels of CR, ALP, ACP and TIAP were related to invasive and metastatic disease as well as with a high Gleason score. Elevated serum levels of CR, ALP, ACP and TIAP, all significantly predicted prognosis in a univariate analysis. In the M0 tumours, ACP and TIAP and TIAP had prognostic value, as they did in the T1-2M0 tumours respectively. Cox's multivariate analysis showed that serum creatinine level at diagnosis had independent prognostic value additional to the TM-classification, Gleason score and patient age. In the M0 tumours, ALP had independent prognostic significance additional to the T-category, Gleason score and patient age. In the T1-2M0 tumours, TIAP had independent prognostic value supplementary to the Gleason score, T-category and patient age, whereas in the T1M0 tumours, the gleason score was an independent prognostic parameter. The results indicate that these simple laboratory tests give important prognostic information in prostatic adenocarcinoma. PMID:7865237

Vesalainen, S; Lipponen, P; Talja, M; Syrjänen, K

1995-01-01

51

Biochemical bases for difference in oxygen affinity of maternal and fetal red blood cells of rattlesnakes.  

PubMed

Pregnancy in Crotalus viridis oreganus is associated with an increase in the nucleoside triphosphate (NTP) concentration and a concomitant decrease in the oxygen affinity of the adult red blood cell. However, although the red blood cells of non-pregnant adults and fetuses have indistinguishable NTP concentrations, they have different oxygen affinities. Therefore, red blood cell NTP concentrations alone cannot account for the oxygen-affinity difference between fetal and maternal red blood cells. Hemoglobins from adult and fetal snakes had similar intrinsic oxygen affinities; however, adult hemoglobin was more responsive to organic phosphate modulation compared with fetal hemoglobin. Structural differences, indicated by native gel electrophoresis and electrophoresis of the globins under denaturing conditions at high pH, corroborated functional differences of hemoglobins from fetus and adult. Therefore, the biochemical basis for the oxygen-affinity difference between maternal and fetal red blood cells in this rattlesnake appears to be unique. It appears to be caused by a functionally distinct fetal hemoglobin and the pregnancy-associated rise in red blood cell NTP levels in the mother. PMID:8457000

Ragsdale, F R; Ingermann, R L

1993-03-01

52

Assessment of gallstone predictor: comparative analysis of ultrasonographic and biochemical parameters  

PubMed Central

Background Gallstones represent a significant burden for health care systems worldwide and are one of the most common disorders presenting to emergency room. Ultrasonography, complete blood picture test and liver function tests are procedures of choice in suspected gallstones or biliary diseases. They are the most sensitive, specific, non-invasive and inexpensive tests for the detection of gallstones. Our main objective was to evaluate the relationship of ultrasonographic findings, hemolytic indices and liver function tests with gallstones. Methodology It was a prospective study carried out in Civil Hospital Karachi (DUHS) and Liaquat National Hospital, two largest tertiary care hospitals of Karachi, Pakistan. Duration of the study was from July 2011 to October 2012. The study was carried out on diagnosed, pre-operative and symptomatic patients of cholelithiases. Exclusion criteria were patients of gallbladder and pancreatic carcinoma, emergency operations, patients having age <12 years and non-cooperative patients, who refused to give written consent for participation in the study. Total two tests were performed on each patient after diagnosis by ultrasonography. These were complete blood count and liver function tests. All the demographic data, laboratory findings and ultrasonographic features were noted in a pre-structured Performa. Sample size was calculated by using open-epidemiological sample size calculator prevalence (p)?=?35%, d?=?5%, and confidence interval (CI) 95%?=?350. All the data was entered and analyzed through SPSS 19. Result There were 454 diagnosed and pre-operative cases of gallstones present in the study. There were 120(26.4%) males and 334(73.6%) females, with a mean age of 42.80?±?12.26 years. Most of the suspects had multiple stones 384 (84.5%) while few had single stones 70(15.4%). Fatty liver was found to be present in 144(31.7%) patients and 92(20.2%) had hepatomegaly. Splenomegaly was present in 16(3.5%) patients. Alkaline phosphatase was elevated in 186(41.0%) patients while SGPT was found to be raised in 160(35.2%). Blood urea nitrogen was found to be elevated in 186(41%) patients and serum creatinine was elevated in 46(10.1%) patients. Conclusion In the light of findings it is recommend that all patients should go through the process of ultrasonography and all the biochemical parameters should be analyzed before surgery. PMID:23618353

2013-01-01

53

Effect of Yucca schidigera extract on blood pressure, antioxidant activity and some blood parameters in the L-name-induced hypertensive rats  

Microsoft Academic Search

Summary: The present study was aimed at investigating the effects of dietary supplementation of Yucca schidigera extract (YSE) on blood pressure, antioxidant activity and some biochemical parameters in the L-NAME-induced hypertensive rats. The study was performed on eighteen male Sprague-Dawley rats divided into 3 groups with 6 animals per group: 1) Control, fed standard chow ad libitum, 2) N-nitro-L-arginine methyl

Aziz BÜLBÜL; Abdullah ERYAVUZ; Gülcan AVCI; ?smail KÜÇÜKKURT; A. Fatih

54

Blood biochemical and cellular changes during a decompression procedure involving eight hours of oxygen prebreathing  

NASA Technical Reports Server (NTRS)

Chemical and cellular parameters were measured in human subjects before and after exposure to a decompression schedule involving 8 h of oxygen prebreathing. The exposure was designed to simulate space-flight extravehicular activity (EVA) for 6 h. Several statistically significant changes in blood parameters were observed following the exposure: increases in calcium, magnesium, osmolality, low-density lipoprotein cholesterol, monocytes, and prothrombin time, and decreases in chloride, creatine phosphokinase and eosinophils. The changes, however, were small in magnitude and blood factor levels remained within normal clinical ranges. Thus, the decompression profile used in this study is not likely to result in blood changes that would pose a threat to astronauts during EVA.

Jauchem, J. R.

1989-01-01

55

Serum Biochemical Parameters in the Ring-Necked Pheasant (Phasianus colchicus) on Breeding Season  

Microsoft Academic Search

5 Abstract: The influence of sex on serum biochemical parameters was investigated in adult (52 week-old) ring-necked pheasants on reproductive activity. Statistical comparisons were made for differences in values between male and female birds. Adult females had higher values for serum total protein and albumin. Significant variations were not observed in the analyses in relation to the sex of the

Elizabeth Moreira dos Santos Schmidt; Antonio Carlos Paulillo; Rosangela Locatelli Dittrich; Elizabeth Santin; Olair Beltrame; Joelma Moura; Edson Goncalves de Oliveira

2007-01-01

56

Effect of starvation on some morphological and biochemical parameters in white sturgeon, Acipenser transmontanus  

Microsoft Academic Search

A 10 week study was conducted to determine the effect of starvation on morphological and biochemical parameters in subyearling white sturgeon. The fish were implanted with passive integrated transponder tags so that body weight change of individual fish could be determined. Ten fish were sampled once every 2 weeks. Carcass:body weight percentage, viscerosomatic index, hepatosomatic index, chemical composition of carcass

Silas S. O. Hung; Wei Liu; Hongbin Li; Trond Storebakken; Yibo Cui

1997-01-01

57

Saline or plant-incorporated methylmercury effects on distribution, demethylation, and blood parameters in rats  

SciTech Connect

The influence of diet is recognized as a significant factor in the expression of toxicity. This applies particularly to toxins like methylmercury (MeHg) which are metabolically incorporated into growing food plants and biotransformed within the plant before ingestion. Methylmercury in this form may influence the early physiological and biochemical events which lead to development of toxicity. In a previous study, a single dose of plant-incorporated methylmercury (MeHg) had a different route of distribution and accumulation in rat organs after 48 h than an equivalent dose of saline MeHg with the greatest accumulation being in red blood cells. Creatine, an important storage form of high energy phosphate in muscles is a primary indicator of erythropoietic dynamics under hypoxia, a sensitive indicator of hemolytic disease, red blood cell aging and impaired marrow efficiency. Since changes in creatine levels occur sooner than changes in other blood parameters, itself being neither synthesized nor metabolized by red blood cells, it can be used as a sensitive indicator of toxicity. The present work investigates the difference in a longer term, multiple-dose regime of saline or bean-incorporated MeHg ingestion with special attention being given to various blood parameters.

Czuba, M.; Komsta-Szumska, E.; Mortimer, D.C.; Champagne, C.

1987-03-01

58

Changes in Serum Mineral Concentrations, Biochemical and Hematological Parameters in Horses with Pica  

Microsoft Academic Search

The aim of this study was to compare hematological, some biochemical parameters, and serum trace element concentrations in\\u000a horses with or without pica. Fifteen horses with pica (group I) and another 15 healthy horses without pica (group II) were\\u000a used. The hematological parameters were not changed between the two groups. In group I, hemoglobin values were lower than\\u000a those of

Ismail Aytekin; Ali Cesur Onmaz; Serap Unubol Aypak; Vehbi Gunes; Osman Kucuk

2011-01-01

59

Biochemical and Hemato-immunological Parameters in Juvenile Beluga (Huso huso) Following the Diet Supplemented with Nettle (Urtica dioica).  

PubMed

The present study investigated the effects of different dietary nettle (Urtica dioica) levels on biochemical, hematological and immunological parameters in beluga (Huso huso). Fish were divided into 4 groups before being fed for 8 weeks with 0%, 3%, 6% and 12% of nettle. The blood samples were collected on week 4 and 8. The use of nettle were not significantly changed the mean cell volume , mean cell haemoglobin, lymphocytes, eosinophils, albumin, glucose, alanine aminotransferase , aspartate aminotransferase, alkaline phosphatase and lysozyme activity on week 4 and 8. After 4 weeks, the total red blood cell (RBC) and haematocrit (Ht) showed a significant increase in 12% nettle group compared to the 3% nettle and control groups but haemoglobin (Hb) had a significant change in 12% nettle compared to the control. At the same time was not found a significant change in the mean cell haemoglobin concentration (MCHC), total white blood cell (WBC), neutrophils, respiratory burst activity (RB), total immunoglobulin (Ig) and total protein (TP), triglyceride (Tri) and cholesterol (Chol). After 8 weeks, the fish treated with nettle exhibited significantly increase in neutrophil and Hb levels compared to the control and between treatment groups, 12% nettle group shown the highest Hb while RBC and Hct values significantly rose in fish fed by 12% compared to the control. Supplementing %6 and 12% nettle increased the WBC and MCHC compared the other groups. The group fed 12% showed a highly significant difference in RB, TP and Ig after 8 weeks. However, Tri and Chol were significantly decreased in the juvenile beluga fed by the %6 and 12% nettle diet compared the other groups. The results suggest that by using this herb there will be an improvement hemato- biochemical parameters and immune function of juvenile beluga. PMID:24513497

Binaii, Mohammad; Ghiasi, Maryam; Mohammad, Seyed; Farabi, Vahid; Pourgholam, Reza; Fazli, Hasan; Safari, Reza; Alavi, Seyed Eshagh; Taghavi, Mohammad Javad; Bankehsaz, Zahra

2013-10-24

60

Biochemical and hemato-immunological parameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica).  

PubMed

The present study investigated the effects of different dietary nettle (Urtica dioica) levels on biochemical, hematological and immunological parameters in beluga (Huso huso). Fish were divided into 4 groups before being fed for 8 weeks with 0%, 3%, 6% and 12% of nettle. The blood samples were collected on week 4 and 8. The use of nettle did not significantly change the mean cell volume, mean cell haemoglobin, lymphocytes, eosinophils, albumin, glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lysozyme activity on week 4 and 8. After 4 weeks, the total red blood cell (RBC) and hematocrit (Ht) showed a significant increase in 12% nettle group compared to the 3% nettle and control groups but haemoglobin (Hb) had a significant change in 12% nettle compared to the control. At the same time was not found a significant change in the mean cell haemoglobin concentration (MCHC), total white blood cell (WBC), neutrophils, respiratory burst activity (RB), total immunoglobulin (Ig) and total protein (TP), triglyceride (Tri) and cholesterol (Chol). After 8 weeks, the fish treated with nettle exhibited significantly increase in neutrophil and Hb levels compared to the control and between treatment groups, 12% nettle group shown the highest Hb while RBC and Hct values significantly rose in fish fed by 12% compared to the control. Supplementing 6% and 12% nettle increased the WBC and MCHC compared to the other groups. The group fed 12% showed a highly significant difference in RB, TP and Ig after 8 weeks. However, Tri and Chol were significantly decreased in the juvenile beluga fed by the 6% and 12% nettle diet compared to the other groups. The results suggest that by using this herb there will be an improvement in hemato-biochemical parameters and immune function of juvenile beluga. PMID:24516872

Binaii, Mohammad; Ghiasi, Maryam; Farabi, Seyed Mohammad Vahid; Pourgholam, Reza; Fazli, Hasan; Safari, Reza; Alavi, Seyed Eshagh; Taghavi, Mohammad Javad; Bankehsaz, Zahra

2014-01-01

61

Hematological and biochemical parameters in apparently healthy Indian population: defining reference intervals.  

PubMed

Clinical reference intervals among Indian population are poorly defined. Therefore, there is an urgent need to establish local clinical laboratory reference intervals for healthy Indian population. The present study aimed to identify the 95 % reference interval for hematological and biochemical parameters in apparently healthy Indian population. We undertook a multicentric cross-sectional study conducted at Apollo Hospitals Educational and Research Foundation across India. Of which 10,665 reference individuals identified as healthy by physicians. The 95 % of the reference distribution was estimated using 2.5th and 97.5th percentile reference limits. The 95 % reference intervals for hemoglobin (Males: 12.3-17 g/dL; Females: 9.9-14.3 g/dL), platelet count (Males: 1.3-3.8; Females: 1.3-4.2 Lakhs/µL), erythrocyte sedimentation rate (Males: 2-22; Females: 4-55 mm/h), serum uric acid in males: 3.5-8.2 mg/dL, gamma glutamyl transferase (Males: 13-61 U/L), fasting blood glucose (Males: 78-110 mg/dL), total cholesterol (Males: 115-254 mg/dL), low density lipoprotein (Males: 60-176 mg/dL) and triglycerides (Males: 55-267 mg/dL, Females: 52-207 mg/dL) were different from currently used reference values. Additionally need for gender based partitioning were observed for triglycerides and gamma glutamyl transferase. The observed findings are of clinical significance and it needs to be validated with additional community based studies. PMID:24966476

Sairam, Shrilekha; Domalapalli, Suhasini; Muthu, Sundaram; Swaminathan, Jayanthi; Ramesh, Vivek A; Sekhar, Lalitha; Pandeya, Palak; Balasubramaniam, Udhaya

2014-07-01

62

Long-term effects of propolis on serum biochemical parameters of rainbow trout (Oncorhynchus mykiss).  

PubMed

Long-term effects of propolis administration on serum biochemical parameters of rainbow trout (Oncorhynchus mykiss) were investigated. To determine the possible toxicity and side effects of propolis, fish were fed on diets containing 0, 0.5, 1.5, 4.5 and 9 g propolis/kg diet for 8 weeks. At the end of the experiment, various seric biochemical parameters were determined. Our results showed that all dosages induced no significant alterations in growth parameters and the seric levels of total protein, albumin, globulin, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol and triglycerides and activities of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, alkaline phosphatase and lactate dehydrogenase, when compared to the control group. On the basis of our findings, propolis is a non-toxic substance for rainbow trout and its long-term administration might not have any side effects. PMID:21030087

Beyraghdar Kashkooli, Omid; Ebrahimi Dorcheh, Eisa; Mahboobi-Soofiani, Nasrollah; Samie, Abdolhossein

2011-03-01

63

The influence of administering "effective microorganisms" to pullets on chosen haematological and biochemical blood indexes.  

PubMed

"Effective Microorganisms" (EM)--a mixture of lactic acid bacteria, photosynthetic bacteria, yeasts and fungi are used mainly in agriculture and organic waste treatment. Recently, they have also been added to water and feed for animals, as well as to processing their excrements into compost and to eliminate the stench. The objective of the present study was to assess the influence of a 14-day administration of an EM solution in drinking water to layer hens on chosen haematological and biochemical indexes. The research was carried out on 120 hens divided into two equal groups. The birds in the experimental group were given drinking water with dissolved EM (5% solution), and those in the control group--water without the preparation. On the 64th day of the aviculture, the hens were weighted and their blood was taken from the wing vein for haematological and biochemical examinations. Administering EM with water to hens did not influence significantly their body weight nor chosen haematological and biochemical indexes. A significant increase was found only in the number of platelets, the level of albumins, the content of total cholesterol and the LDH activity, however, a decrease in the ALT activity was observed. PMID:20169927

Sokó?, R; Michalczyk, M; Spodniewska, A; Barski, D

2009-01-01

64

Effect of a glyphosate-based herbicide in Cyprinus carpio: assessment of acetylcholinesterase activity, hematological responses and serum biochemical parameters.  

PubMed

The objective of this study was to investigate the toxicity effects of acute and sublethal of Roundup® as a glyphosate-based herbicide on acetylcholinesterase (AChE) activity and several hematological and biochemical parameters of Cyprinus carpio. The LC??-96 h of Roundup® to C. carpio was found to be 22.19 ppm. Common carp was subjected to Roundup® at 0 (control), 3.5, 7 and 14 ppm for 16 days, and the AChE activity is verified in tissues of gill, muscle, brain and liver. After 5 days, a significant decrease was observed in the AChE activity of muscle, brain and liver tissues. Besides, a time- and dose-dependent increase in mean cell hemoglobin (MCH) and mean cell volume (MCV) was observed. In contrast, a significant decrease was found in the quantities of hemoglobin (Hb), hematocrit (HCT) and, red (RBC) and white (WBC) blood cell count. Also, the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) in Roundup® treated groups were significantly higher than the controlled group at experimental periods. However, the level of alkaline phosphatase (ALP) had a significant reduction behavior during the sampling days. It seems that the changes in hematological and biochemical parameters as well as AChE activity could be used as efficient biomarkers in order to determine Roundup® toxicity in aquatic environment. PMID:24075644

Gholami-Seyedkolaei, Seyed Jalil; Mirvaghefi, Alireza; Farahmand, Hamid; Kosari, Ali Asghar

2013-12-01

65

Trigonella foenum-graecum protection against deltamethrin-induced toxic effects on haematological, biochemical, and oxidative stress parameters in rats.  

PubMed

Trigonella foenum-graecum L. is enriched with many active ingredients. TFG oil was evaluated for its protective effect against deltamethrin toxicity in rats. Rats of the control group were administered saline. The 2nd group was administered deltamethrin (DLM) orally at a concentration of 15 mg/kg body mass. The 3rd and 4th groups were administered DLM at a concentration of 15 mg/kg body mass and were fed diets containing 2.5% and 5% TFG oil, respectively. DLM intoxication reduced red blood cell and platelet counts, hemoglobin concentration, and hematocrit value while it induced leucocytosis. Furthermore, it increased serum levels of lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, ?-glutamyltransferase, triglycerides, cholesterol, uric acid, urea, and creatinine; increased hepatic, renal, and brain lipid peroxidation; decreased serum acetylcholine esterase level; and decreased hepatic, renal, and brain antioxidant markers' activities. However, TFG oil kept the studied hematological and biochemical parameters within normal ranges. In addition, it prevented lipid peroxidation and oxidative stress induced by DLM intoxication in a dose-dependent manner. Therefore, these results indicated that TFG oil inhibited the toxic effects of DLM on hematological and biochemical parameters as well as oxidative status by its free radical scavenging and potent antioxidant activities, and it appeared to be a promising protective agent against DLM-induced toxicity. PMID:25029214

Abdel-Daim, Mohamed M; Abd Eldaim, Mabrouk A; Mahmoud, Mohamed M

2014-08-01

66

[The blood biochemical indicators as possible markers of destruction of kerato-hyaline tissue of nails in patients with onycholysis].  

PubMed

It is established that the most informative blood biochemical indicators of destructive process in patients with onycholysis are to be considered concentration of oxiproline in blood. The changing of ratio of fractions oxiproline, balance between concentration of enzymes of antioxidant defense and metalloproteinase and concentration of hemoglobin in blood make it possible to diagnose the mixed type of hypoxemia which is a possible cause of destruction of kerato-hyaline tissue in patients with onycholysis. PMID:23479963

Urazovskaia, E V; Mikashinovich, Z I

2012-12-01

67

Blood, urine and vitreous isopropyl alcohol as biochemical markers in forensic investigations.  

PubMed

Isopropyl alcohol (IPA) is widely used as an industrial solvent and cleaning fluid. After ingestion or absorption, IPA is converted into acetone by alcohol dehydrogenase. However, in ketosis, acetone can be reduced to IPA. The aim of this study was to investigate blood IPA and acetone concentrations in a series of 400 medico-legal autopsies, including cases of diabetic ketoacidosis, hypothermia and alcohol misuse-related deaths, to illustrate the extent of ketosis at the time of death. Vitreous glucose, blood 3-?-hydroxybutyrate (3HB) and acetoacetate (AcAc) concentrations were also determined systematically. Additionally, vitreous and urine IPA, acetone, 3HB and AcAc concentrations as well as other biochemical markers, including glycated hemoglobin and carbohydrate-deficient transferrin (CDT) were also determined in selected cases. The results of this study indicate that ketosis is characterized by the presence of IPA resulting from the acetone metabolism and that IPA can be detected in several substrates. These findings confirm the importance of the systematic determination of IPA and acetone levels that is used to quantify biochemical disturbances and the importance of ketosis at the time of death. PMID:22177827

Palmiere, Cristian; Sporkert, Frank; Werner, Dominique; Bardy, Daniel; Augsburger, Marc; Mangin, Patrice

2012-01-01

68

Stability of Intercellular Exchange of Biochemical Substances Affected by Variability of Environmental Parameters  

NASA Astrophysics Data System (ADS)

Communication between cells is realized by exchange of biochemical substances. Due to internal organization of living systems and variability of external parameters, the exchange is heavily influenced by perturbations of various parameters at almost all stages of the process. Since communication is one of essential processes for functioning of living systems it is of interest to investigate conditions for its stability. Using previously developed simplified model of bacterial communication in a form of coupled difference logistic equations we investigate stability of exchange of signaling molecules under variability of internal and external parameters.

Mihailovi?, Dragutin T.; Budin?evi?, Mirko; Balaž, Igor; Mihailovi?, Anja

69

Effects of some plants of the spurge family on haematological and biochemical parameters in rats  

Microsoft Academic Search

AdEdApO, A. A., M. O. AbA t An, O. O. OlOrunsOgO: Effects of some plants of the spurge family on haematological and biochemical parameters in rats. Vet. arhiv 77, 29-38, 2007. AbstrAct The effects of five suspected poisonous plants of the spurge family ( euphorbiaceae ) i.e. alchornea cordifolia Schum and Thorn, Cnidoscolus acontifolius Mill, Phyllanthus amarus Schum and Thorn,

Adeolu Alex Adedapo; Matthew Oluwole Abatan; Olufunso Olabode Olorunsogo

70

Effects of drinking diesel-contaminated water on the hematological and serum biochemical parameters of mice  

Microsoft Academic Search

Pollution of the environment by petroleum products is a common feature in oil producing nations, especially in the developing\\u000a countries where the incidence of pipe line leakage and valdalization is very common. This study investigated the effects of\\u000a exposure to water contaminated with very low concentration of diesel on some hematological and serum biochemical parameters\\u000a of mice. A total of

Samuel Chukwuneke Udem; Isaac A. M. Lekwuwa; Ngozi D. Udem

2011-01-01

71

The influences of SE infection on layers’ production performance, egg quality and blood biochemical indicators  

PubMed Central

Background Salmonella enterica serovar Enteritidis (SE), as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers’ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into 2 groups, 60 hens for experiment and others for control. The experiment group was fed with the dosage of 108 CFU SE per hen. The specific PCR was used to detect the deposition of SE. On the 8 d after SE infection, 10 hens from the control group and 30 hens from the experimental group were slaughtered to detect the SE colonization. The production performance, egg quality and blood biochemical indices were also analyzed. Results The results showed that the colonization rate of SE was highest in caecum contents (55.17%) and lowest in vagina (17.24%). For the eggs the detection rate of SE was highest on the eggshell (80.00%) and lowest in yolk (18.81%). SE infection had no significant influence on production performance and egg qualities (P > 0.05). The difference of laying rate between the experimental and control groups was less than 0.30%, and both were approximately equal to 82.00%. The blood analysis showed that the aspartic aminotransferase (AST) and alanine aminotransferase (ALT) of experimental group was significantly higher than those of control group (P < 0.05). For experimental and control groups AST values were 236.22 U/l and 211.84 U/l respectively, and ALT values were 32.19 U/l and 24.55 U/l. All of coefficients were less than 20%. The colonization of SE in organs increases the enzyme activities of AST and ALT in blood. Conclusions SE in feed could invade the oviduct and infect the forming eggs. It significantly increased the concentration of ALT and AST in blood. However,SE infection was hard to be observed from the appearances of layer and egg. It might be a dangerous risk to human health. PMID:24405886

2014-01-01

72

Comparative aspects of biochemical polymorphism in the blood of Caprinae species and their hybrids.  

PubMed

Biochemical variation at 14 blood loci was reviewed, and specific features compared experimentally in sheep Ovis aries, mouflon Ovis musimon, goat Capra hircus, aoudad Ammotragus Iervia and in 2 stillborn aoudad X goat hybrids. Variation at 3 loci was also studied in dall sheep Ovis dalli, bighorn sheep Ovis canadensis and rocky mountain goat Oreamnos americanus. Haemoglobin C production in an anaemic Hb AB mouflon and in mouflon X sheep hybrids was examined. Mouflon differ from domestic sheep in that synthesis of both Hb beta A and Hb beta B chains is switched off during Hb C production. The mouflon X sheep hybrids switched off one or both chains depending on whether they had inherited sheep or mouflon Hb beta chain genes. In general aoudad showed a closer affinity to goats than to sheep. PMID:7458003

Tucker, E M; Clarke, S W

1980-01-01

73

Effect of gender, biochemical parameters & parathyroid surgery on gastrointestinal manifestations of symptomatic primary hyperparathyroidism  

PubMed Central

Background & objectives: Information on gastrointestinal manifestations and then response after curative parathyroid surgery is scarce in symptomatic primary hyperparathyroidism (PHPT). This study was carried out to analyse gastrointestinal manifestations in patients with PHPT and their associations with biochemical parameters. Methods: This retrospective study included 153 patients with symptomatic primary hyperparathyroidism (PHPT). The signs and symptoms pertaining to gastrointestinal system were analyzed. The difference of symptoms between men and women and difference in biochemical parameters in presence of different symptoms were evaluated. The relationship between serum calcium, phosphate and parathyroid hormone (PTH) levels with presence of gallstone and pancreatitis was also studied. Result: Of the 153 patients, 46 (30%) were men. The mean age was 39.2 ± 13.9 yr. Nearly 80 per cent of PHPT patients had at least one symptom/ sign related to gastrointestinal system. The most common gastrointestinal manifestations were abdominal pain 66 (43%), constipation 55 (36%), and nausea/or vomiting 46 (30%). Nearly one-fourth 34 (22%) of patients had a history of either gallstone disease or cholecystectomy or both. The prevalence of gallstone disease was higher in women (P<0.05). Imaging and biochemical evidence of pancreatitis was found in 27 (18%) patients. Pancreatitis was more common in men compared to women (P<0.05) despite the higher prevalence of gallstones in women. Serum calcium, phosphate or PTH levels were not associated with high risk for gallstone disease, however, serum calcium (P<0.05) was associated with 1.3 times higher risk of developing pancreatitis. In majority of patients, gastrointestinal manifestations resolved within three months of curative parathyroidectomy. Except two patients, none had recurrence of pancreatitis. Interpretation & conclusions: The study revealed that the gastrointestinal symptoms were common in patients with symptomatic PHPT. There was not much gender difference in gastrointestinal symptoms except higher occurrence of gallstones in women and pancreatitis in men. There was no difference in biochemical profile between those who had and did not have gastrointestinal symptoms. PMID:24718404

Shah, Viral N.; Bhadada, Sanjay Kumar; Bhansali, Anil; Behera, A.; Bhattacharya, Anish; Nahar, Uma; Bhasin, Deepak; Vadera, Bhavin

2014-01-01

74

Heat tolerance in Brazilian sheep: physiological and blood parameters.  

PubMed

Thirty Santa Ines adult, non-lactating, non-pregnant ewes, ten with a brown coat, ten black coated and ten white coated, as well as ten Bergamasca and ten of mixed breed were used to evaluate the effect of climate on physiological and blood parameters in sheep. Two sample collections were taken (6 AM: and 2 PM: ) on six days. Sweating rate (SR), heart (HR) and breathing rates (BR), complete hemogram, rectal (RT) and skin temperatures (ST) were measured. Variance analyses were carried out using SAS(R). In general, there were significant differences between animals due to skin type, time and day of collection. White coated animals showed lower HR, BR and RT, with afternoon parameters higher than morning. Correlations between HR, BR, RT, SR and ST were medium and positive. Correlations between physiological and blood traits were in general negative and medium. Packed Cell Volume (PCV), total plasma proteins, red blood cell count and hemoglobin concentration had high positive correlations between each other. The first two autovectors explained 49% of variation between traits. White coated Santa Ines animals were shown to be better adapted to climatic conditions in Central Brazil and wool sheep more affected by heat. PMID:19052907

McManus, Concepta; Paludo, Giane Regina; Louvandini, Helder; Gugel, Rosilene; Sasaki, Luiz Cláudio Bastos; Paiva, Samuel Rezende

2009-01-01

75

Spent mushroom substrate influences elk (cervus elaphus canadensis) hematological and serum biochemical parameters.  

PubMed

The objective of this study was to evaluate the effect of spent mushroom substrate (SMS) derived from Pleurotus eryngii on the hematological and biochemical blood properties of elk. A total of 18, two and three-year-old elk were fed three different levels of SMS (0, 15 and 20%) in a corn-wheat bran diet for 80 days. The results indicated significantly high levels of blood monocytes, hemoglobin (Hb), and hematocrit (HCT) in elk fed 15% or 20% SMS (p<0.05) compared to control animals. Serum blood urea nitrogen (BUN) and glucose concentrations were also significantly elevated in elk fed both 15% and 20% SMS. The inclusion of SMS in the elk diet did not affect serum total cholesterol, triglyceride, or low density lipoprotein (LDL)-cholesterol concentrations; however, high density lipoprotein (HDL)-cholesterol concentration was significantly increased in SMS-fed groups. In addition, 20% SMS in the diet increased serum iron and testosterone concentrations in elk. These results indicate that adding SMS to the diet of elk can increase their Hgb, serum BUN, glucose, and HDL-cholesterol concentration; therefore, diets containing SMS may enhance the physiologic condition of elk during growth. PMID:25049569

Park, Jae Hong; Kim, Sang Woo; Do, Yoon Jung; Kim, Hyun; Ko, Yeoung Gyu; Yang, Boh Suk; Shin, Daekeun; Cho, Young Moo

2012-03-01

76

Spent Mushroom Substrate Influences Elk (Cervus Elaphus Canadensis) Hematological and Serum Biochemical Parameters  

PubMed Central

The objective of this study was to evaluate the effect of spent mushroom substrate (SMS) derived from Pleurotus eryngii on the hematological and biochemical blood properties of elk. A total of 18, two and three-year-old elk were fed three different levels of SMS (0, 15 and 20%) in a corn-wheat bran diet for 80 days. The results indicated significantly high levels of blood monocytes, hemoglobin (Hb), and hematocrit (HCT) in elk fed 15% or 20% SMS (p<0.05) compared to control animals. Serum blood urea nitrogen (BUN) and glucose concentrations were also significantly elevated in elk fed both 15% and 20% SMS. The inclusion of SMS in the elk diet did not affect serum total cholesterol, triglyceride, or low density lipoprotein (LDL)-cholesterol concentrations; however, high density lipoprotein (HDL)-cholesterol concentration was significantly increased in SMS-fed groups. In addition, 20% SMS in the diet increased serum iron and testosterone concentrations in elk. These results indicate that adding SMS to the diet of elk can increase their Hgb, serum BUN, glucose, and HDL-cholesterol concentration; therefore, diets containing SMS may enhance the physiologic condition of elk during growth. PMID:25049569

Park, Jae Hong; Kim, Sang Woo; Do, Yoon Jung; Kim, Hyun; Ko, Yeoung Gyu; Yang, Boh Suk; Shin, Daekeun; Cho, Young Moo

2012-01-01

77

Cardio-respiratory, haematological and biochemical parameter adjustments to exercise: effect of a probiotic in horses during training.  

PubMed

Two randomly distributed groups of thoroughbred horses were compared during a 12-week period for their cardio-respiratory and metabolic adjustment to strenuous exercise, training and detraining. The horses were trained following the same standardized schedule and were regularly investigated using standardized treadmill exercise tests (SET) of increasing speed. After the first SET and during the whole experimental period, a group of 6 horses received a probiotic (Bioracing) once a day while a group of 5 horses received a placebo. All other conditions were similar for both groups. During each SET, the oxygen uptake, carbon dioxide output, tidal volume (inspired volume), respiratory rate and expired minute volume were obtained using 2 ultrasonic pneumotachographs and a mass spectrometer. All the parameters were the mean of the values calculated during the last 20 s of the SET. Heart rate was continuously measured with a polar horse tester. Venous blood was sampled before and after the test and analyzed for various biochemical parameters. In both groups, training induced significant modification in most of the cardio-respiratory parameters, ie peak oxygen uptake, peak carbon dioxide output, respiratory exchange ratio, ventilation/min to oxygen-uptake ratio and oxygen-uptake to heart-rate ratio. After the 3-week detraining period, most of the values were again similar to the pre-training values in both groups. However, the training-induced modifications of most of the cardio-respiratory parameters occurred earlier and were proportionally greater in the probiotic-treated group than in the control. The respiratory coefficient decreased in the control but not in the treated group. All other parameters changed similarly in both groups. This suggests that Bioracing could modify the physiological effects of training by improving some aerobic metabolic capacities for carbohydrate utilization, but that this effect occurs only during training and not during periods of physical inactivity. PMID:8087145

Art, T; Votion, D; McEntee, K; Amory, H; Linden, A; Close, R; Lekeux, P

1994-01-01

78

Gut microbiota composition is associated with body weight, weight gain and biochemical parameters in pregnant women.  

PubMed

Obesity is associated with complications during pregnancy and increased health risks in the newborn. The objective of the present study was to establish possible relationships between gut microbiota, body weight, weight gain and biochemical parameters in pregnant women. Fifty pregnant women were classified according to their BMI in normal-weight (n 34) and overweight (n 16) groups. Gut microbiota composition was analysed by quantitative real-time PCR in faeces and biochemical parameters in plasma at 24 weeks of pregnancy. Reduced numbers of Bifidobacterium and Bacteroides and increased numbers of Staphylococcus, Enterobacteriaceae and Escherichia coli were detected in overweight compared with normal-weight pregnant women. E. coli numbers were higher in women with excessive weight gain than in women with normal weight gain during pregnancy, while Bifidobacterium and Akkermansia muciniphila showed an opposite trend. In the whole population, increased total bacteria and Staphylococcus numbers were related to increased plasma cholesterol levels. Increased Bacteroides numbers were related to increased HDL-cholesterol and folic acid levels, and reduced TAG levels. Increased Bifidobacterium numbers were related to increased folic acid levels. Increased Enterobacteriaceae and E. coli numbers were related to increased ferritin and reduced transferrin, while Bifidobacterium levels showed the opposite trend. Therefore, gut microbiota composition is related to body weight, weight gain and metabolic biomarkers during pregnancy, which might be of relevance to the management of the health of women and infants. PMID:20205964

Santacruz, A; Collado, M C; García-Valdés, L; Segura, M T; Martín-Lagos, J A; Anjos, T; Martí-Romero, M; Lopez, R M; Florido, J; Campoy, C; Sanz, Y

2010-07-01

79

Effects of the surfactant polyoxyethylene amine (POEA) on genotoxic, biochemical and physiological parameters of the freshwater teleost Prochilodus lineatus.  

PubMed

The surfactant polyoxyethylene amine (POEA) is added to several formulations of glyphosate herbicides that are widely used in agriculture and can contaminate aquatic ecosystems. In the present study, an integrated approach examining genotoxic, biochemical and physiological parameters was employed to evaluate acute effects of POEA on the Neotropical fish Prochilodus lineatus. Juvenile fish were exposed to 0.15 mg·L(-1) (POEA 1), 0.75 mg·L(-1) (POEA 2) and 1.5 mg·L(-1) (POEA 3) of POEA or only water (CTR), and after 24h exposure samples of blood and liver were taken. Compared with CTR, liver of fish exposed to POEA 2 and POEA 3 showed increased activity of 7 ethoxyresorufin-O-deethylase and increased content of glutathione, whereas the activity of glutathione-S-transferase was diminished. On the other hand, fish of the group POEA 1 showed an increase in the activity of superoxide dismutase and in the occurrence of lipid peroxidation. Fish exposed to POEA 3 presented increased hepatic activity of glutathione peroxidase and reduced plasma cortisol. The exposure to POEA at all concentrations tested caused an increase in plasma lactate and a decrease in the hepatic activity of catalase, in the number of red blood cells and in hemoglobin content. The comet assay used for analyzing DNA damage in blood cells indicated the genotoxicity of the surfactant at all concentrations tested. Taken together these results show that POEA can cause effects at various levels, such as hemolysis, DNA damage and lipid peroxidation, which are directly related to an imbalance in the redox state of the fish. PMID:24955954

Navarro, Claudia D C; Martinez, Claudia B R

2014-09-01

80

Effects of Lichenic Extracts (Hypogymnia physodes, Ramalina polymorpha and Usnea florida) on Human Blood Cells: Cytogenetic and Biochemical Study  

PubMed Central

Several lichen species have been used for medicinal purposes throughout the ages, and they were reported to be effective in the treatment of different disorders including tuberculosis, hemorrhoids, ulcer, dysentery and cancer. It is revealed that they may be easily accessible sources of natural drugs that could be used as a possible food supplement or in pharmaceutical industry after their safety evaluations. However, so far, the nature and/or biological roles of plenty of lichenes have not been elucidated exactly. The aim of this study was to investigate the genetic and oxidative effects of water extracts of three different lichen species; Hypogymnia physodes, Ramalina polymorpha and Usnea florida in cultured human blood cells (n = 5) for the first time. All lichen species were collected from the Erzurum and Artvin provinces (in Turkey) during August 2010. The lichen extracts were added into culture tubes at various concentrations (0 to 2000 mg/L). Chromosome aberrations (CA) and micronucleus (MN) tests were used for genotoxic influences estimation. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative stress (TOS)) were examined to determine oxidative effects. In our in-vitro test systems, it was observed that all tested lichen extracts had no mutagenic effects on human lymphocytes. Furthermore, these extracts exhibited antioxidant properties due to the type of lichen species added to the cultures. In conclusion, these lichens can be a new resource of therapeutics as recognized in this study with their non-mutagenic and antioxidant features. PMID:24250516

Türkez, Hasan; Ayd?n, Elanur; Aslan, Ali

2012-01-01

81

Could selenium administration alleviate the disturbances of blood parameters caused by lithium administration in rats?  

PubMed

Lithium is widely used in medicine, but its administration can cause numerous side effects. The present study aimed at the evaluation of the possible application of selenium, an essential and antioxidant element, as a protective agent against lithium toxicity. The experiment was performed on four groups of Wistar rats: I (control)-treated with saline, II (Li)-treated with lithium (Li2CO3), III (Se)-treated with selenium (Na2SeO3) and IV (Li?+?Se)-treated with lithium and selenium (Li2CO3 and Na2SeO3) in the form of water solutions by stomach tube for 6 weeks. The following biochemical parameters were measured: concentrations of sodium, potassium, calcium, magnesium, phosphorus, iron, urea, creatinine, cholesterol, glucose, total protein and albumin and activities of alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase in serum as well as whole blood superoxide dismutase and glutathione peroxidase. Morphological parameters such as red blood cells, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelets, white blood cells, neutrophils as well as lymphocytes were determined. Lithium significantly increased serum calcium and glucose (2.65?±?0.17 vs. 2.43?±?0.11; 162?±?31 vs. 121?±?14, respectively), whereas magnesium and albumin were decreased (1.05?±?0.08 vs. 1.21?±?0.15; 3.85.?±?0.12 vs. 4.02?±?0.08, respectively). Selenium given with lithium restored these parameters to values similar to those observed in the control (Ca-2.49?±?0.08, glucose-113?±?26, Mg-1.28?±?0.09, albumin-4.07?±?0.11). Se alone or co-administered with Li significantly increased aspartate aminotransferase and glutathione peroxidase. The obtained outcomes let us suggest that the continuation of research on the application of selenium as an adjuvant in lithium therapy seems warranted. PMID:24676629

Kie?czykowska, Ma?gorzata; Kocot, Joanna; Kurzepa, Jacek; Lewandowska, Anna; ?elazowska, Renata; Musik, Irena

2014-06-01

82

Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice  

PubMed Central

Facial vein (cheek blood) and caudal vein (tail blood) phlebotomy are two commonly used techniques for obtaining blood samples from laboratory mice, while automated blood sampling through a permanent catheter is a relatively new technique in mice. The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24?h by cheek blood, tail blood or automated blood sampling from the carotid artery. Mice subjected to cheek blood sampling lost significantly more body weight, had elevated levels of plasma corticosterone, excreted more fecal corticosterone metabolites, and expressed more anxious behavior than did the mice of the other groups. Plasma corticosterone levels of mice subjected to tail blood sampling were also elevated, although less significantly. Mice subjected to automated blood sampling were less affected with regard to the parameters measured, and expressed less anxious behavior. We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling. The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff. PMID:24958546

Kalliokoski, Otto; Sørensen, Dorte B; Hau, Jann; Abelson, Klas S P

2014-01-01

83

Meta-analysis of the relationship of mycotoxins with biochemical and hematological parameters in broilers.  

PubMed

A meta-analysis was carried out to study the association of mycotoxins with hematological and biochemical profiles in broilers. Ninety-eight articles published between 1980 and 2009 were used in the database, totaling 37,371 broilers. The information was selected from the Materials and Methods and Results sections in the selected articles and then tabulated in a database. Meta-analysis followed 3 sequential analyses: graphic, correlation, and variance-covariance. Mycotoxins reduced (P < 0.05) the hematocrit (-5%), hemoglobin (-15%), leukocytes (-25%), heterophils (-2%), lymphocytes (-2%), uric acid (-31%), creatine kinase (-27%), creatinine (-23%), triglycerides (-39%), albumin (-17%), globulin (-1%), total cholesterol (-14%), calcium (-5%), and inorganic phosphorus (-12%). Mycotoxins also altered (P < 0.05) the concentrations of alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. A quadratic effect was observed on the relationship between the concentration of aflatoxin in diets and the serum concentration of alkaline phosphatase, ?-glutamyl transferase, alanine aminotransferase, and aspartate aminotransferase. The total protein concentration in blood was 18% lower (P < 0.05) in broilers challenged by aflatoxins compared with that of the unchallenged ones. The inclusion of antimycotoxin additives in diets with aflatoxins altered (P < 0.05) some variables (uric acid, creatinine, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and ?-glutamyl transferase) in relation to the group that received diets with the mycotoxin and without the additive. The meta-analysis performed in this study allowed us to address and quantify systematically the relationship of mycotoxins with alterations in hematologic and biochemical profiles in broilers. PMID:22252351

Andretta, I; Kipper, M; Lehnen, C R; Lovatto, P A

2012-02-01

84

Blood flow quantification using 1D CFD parameter identification  

NASA Astrophysics Data System (ADS)

Patient-specific measurements of cerebral blood flow provide valuable diagnostic information concerning cerebrovascular diseases rather than visually driven qualitative evaluation. In this paper, we present a quantitative method to estimate blood flow parameters with high temporal resolution from digital subtraction angiography (DSA) image sequences. Using a 3D DSA dataset and a 2D+t DSA sequence, the proposed algorithm employs a 1D Computational Fluid Dynamics (CFD) model for estimation of time-dependent flow values along a cerebral vessel, combined with an additional Advection Diffusion Equation (ADE) for contrast agent propagation. The CFD system, followed by the ADE, is solved with a finite volume approximation, which ensures the conservation of mass. Instead of defining a new imaging protocol to obtain relevant data, our cost function optimizes the bolus arrival time (BAT) of the contrast agent in 2D+t DSA sequences. The visual determination of BAT is common clinical practice and can be easily derived from and be compared to values, generated by a 1D-CFD simulation. Using this strategy, we ensure that our proposed method fits best to clinical practice and does not require any changes to the medical work flow. Synthetic experiments show that the recovered flow estimates match the ground truth values with less than 12% error in the mean flow rates.

Brosig, Richard; Kowarschik, Markus; Maday, Peter; Katouzian, Amin; Demirci, Stefanie; Navab, Nassir

2014-03-01

85

The Clinical and Biochemical Parameters in Relation to the Serum Neopterin Levels in Indian Children and Adolescents  

PubMed Central

Introduction: An adverse pattern of blood lipids and cardiovascular abnormalities starts in obese children during childhood and neopterin serves as a marker for cardiovascular disease. Unfortunately, the data for children and adolescents, particularly, in the Indian population, are scarce. The present study aimed at evaluating the levels of serum neopterin in obese and overweight children and adolescents of the Indian population. Methods: The study groups included 296 school going children and adolescents. (96 obese and 97 overweight subjects were compared with 103 normal controls who were aged between 10-17 years). The anthropometric variables, the lipid profile, the fasting serum glucose which was analyzed by using an autoanalyzer and the serum neopterin levels were assayed by HPLC (Shimazdu) by using the method of Palfrey et al., 1993. The serum insulin levels were measured by using ELISA kits. Results: The serum neopterin levels (nmol/l) were elevated significantly in the obese (7.4±1.4) and overweight (6.4±0.8) (p<0.001) children and adolescents than in the controls (4.9±0.9). The serum neopterin levels showed a positive correlation with the BMI (r=0.79), WHR (r=0.5), systolic (r=0.44) and diastolic blood pressures (r= 0.25), insulin (r=0.57), HOMAIR (r=0.55), total cholesterol (r=0.35), triglycerides (r=0.20) and LDL-C (r=0.27) and they showed a negative correlation with HDL-C (r=-0.15) and fasting glucose (r= -0.3). Conclusion: This study revealed a good relationship between serum neopterin and the anthropometric and biochemical parameters. We, therefore, aim to conduct regular camps at schools to counsel and advise the identified overweight and obese children to go for physical exercise and a balanced diet. The implementation of preventive measures from early childhood will have far reaching benefits, as even the prevalence of other obesity related disorders could decline. PMID:23730631

Nageswara Rao G., Srinivasa; Gurumurthy, Prema; E., Sruti; Cherian, K.M.

2013-01-01

86

Changes in growth, photosynthetic activities, biochemical parameters and amino acid profile of Thompson Seedless grapes (Vitis vinifera L.).  

PubMed

The study on photosynthetic activity and biochemical parameters in Thompson Seedless grapes grafted on Dog Ridge rootstock and its impact on growth, yield and amino acid profile at various stages of berry development was conducted during the year 2012-2013. Leaf and berry samples from ten year old vines of Thompson Seedless were collected at different growth and berry developmental stages. The analysis showed difference in photosynthetic activity, biochemical parameters and amino acid status with the changes in berry development stage. Higher photosynthetic rate of 17.39 umol cm(-2) s(-1) was recorded during 3-4mm berry size and the lowest (10.08 umol cm(-2) s(-1)) was recorded during the veraison stage. The photosynthetic activity showed gradual decrease with the onset of harvest while the different biochemical parameters showed increase and decrease from one stage to another in both berry and leaves. Changes in photosynthetic activity and biochemical parameters thereby affected the growth, yield and amino acid content of the berry. Positive correlation of leaf area and photosynthetic rate was recorded during the period of study. Reducing sugar (352.25 mg g(-1)) and total carbohydrate (132.52 mg g(-1)) was more in berries as compared to leaf. Amino acid profile showed variations in different stages of berry development. Marked variations in photosynthetic as well as biochemical and amino acid content at various berry development stages was recorded and thereby its cumulative effect on the development of fruit quality. PMID:25522520

Somkuwar, R G; Bahetwar, Anita; Khan, I; Satisha, J; Ramteke, S D; Itroutwar, Prerna; Bhongale, Aarti; Oulkar, Dashrath

2014-11-01

87

[Experimental paroxysmal hemoglobinuria in calves and selected biochemical indicators in the blood and urine].  

PubMed

When examining diseased calves, sporadically pronounced haemoglobinuria with dark red urine can be observed. In serious cases the clinical picture may be manifold but peculiar; in easy cases, however, when there are no distinct clinical symptoms, a larger scale of examinations is needed to aid differential diagnosis. Eight roughage-fed bulls aged two months, weighing 55-71 kg were used in this experiment. Selected biochemical indices of the mineral, enzymatic, hepatic, energetic and urinary profile were determined in the blood serum and urine of the animals. After the administration of cold water at an amount representing 12% of the animal's body weight, ionogram values were determined. In all indices a positive correlation with hydraemia and a decrease in Na, Cl, Ca, Mg and P levels were observed. Correction of the above levels occurred within 24 hours, with the exception of Na and P concentrations that did not reach starting values. As to the enzymatic profile (AST, ALT, GGT), no pronounced disturbances could be observed. The most profound changes were seen in AST activity that increased in the 5th hour of the experiment. A slight tendency towards hypoproteinaemia was observed to continue even in 24 hours. Hypoglobulinaemia reached its starting value in the 24th hour while simultaneously albumin levels slightly increased. The increasing bilirubin levels reached their maximum in the 5th and 6th hour; correction of the former occurred within 24 hours. The urinary profile revealed polyuria, aciduria, aquaeous urine and haemoglobinuria, the latter reaching its peak between hours 1 and 3 following water administration.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:8236629

Sedovic, M; Nagy, O; Slanina, L

1993-01-01

88

The relationship between stroke mortality and red blood cell parameters  

PubMed Central

Background: Several factors influence on the outcome of ischemic stroke. The aim of this study was determination the relationship between stroke mortality and red blood cell parameters. Methods: This cross-sectional study was conducted from 2011 July to June 2012. For all patients with ischemic stroke in middle cerebral artery (MCA) territory, the cell blood count test was performed. We recorded their mortality on the 1st week and the 1st month after ischemic stroke. Data analysis was performed using t-test, ?2, Mann–Whitney U-test, logistic regression and receiver operating characteristic curve in SPSS for Windows 19.0. Results: A total of 98 subjects (45.9% men and 54.1% women) with the mean age of 71.0 ± 13.9 years were assessed, while 67.3% of them were anemic. The prevalence of 1st week mortality among anemic and non-anemic patients was 40.9% and 34.4% (P = 0.534). The prevalence of mortality after 1st week till 1st month was 19.6% and 21.0% respectively (P = 0.636). In univariant analysis, only 1st month mortality had a significant relationship with red blood cell (RBC) count (P = 0.022). However, the result of logistic regression model showed that RBC (P = 0.012) and mean corpuscular volume (MCV) (P = 0.021) remained as predictors of the 1st week and the 1st month mortality (P = 0.011 and P = 0.090 respectively). The best cutoff point of RBC for the prediction of the 1st week mortality with 44.7% specificity and 69.5% sensitivity was estimated 4.07 million/?l and for the 1st month mortality with 46.6% specificity and 72.2% sensitivity was estimated 4.16 million/?l. Conclusion: The RBC count and MCV are independent predictors of ischemic stroke short-term mortality.

Hatamian, Hamidreza; Saberi, Alia; Pourghasem, Matin

2014-01-01

89

Field-effect-based multifunctional hybrid sensor module for the determination of both (bio-)chemical and physical parameters  

NASA Astrophysics Data System (ADS)

Sensor systems for multi-parameter detection in fluidics usually combine different sensors, which are designed to detect either a physical or (bio-)chemical parameter. Therefore, such systems include a more complicated fabrication technology and measuring set-up. In this work, an ISFET (ion-sensitive field-effect transistor), which is well known as a (bio-)chemical sensor, is utilized as transducer for the detection of both (bio-)chemical and physical parameters. A multifunctional hybrid module for the determination of two (bio-)chemical parameters (pH, penicillin concentration) and three physical parameters (temperature, flow velocity and flow direction) using only two sensor structures, an ion generator and a reference electrode, is realized and its performance has been investigated. Here, a multifunctionality of the sensor system is achieved by means of different sensor arrangements and/or different operation modes. A Ta2O5-gate ISFET was used as transducer for all sensors. A novel time-of-flight type ISFET-based flow-velocity (flow rate) and flow-direction sensor using in-situ electrochemical generation of chemical tracers is presented. Due to the fast response of the ISFET (usually in the millisecond range), an ISFET-based flow sensor is suitable for the measurement of the flow velocity in a wide range. With regard to practical applications, pH measurements with this ISFET were performed in rain droplets.

Schoening, Michael J.; Poghossian, Arshak; Schultze, J. Walter; Lueth, Hans

2002-02-01

90

Characterization of blood biochemical markers during aging in the Grey Mouse Lemur (Microcebus murinus): impact of gender and season  

PubMed Central

Background Hematologic and biochemical data are needed to characterize the health status of animal populations over time to determine the habitat quality and captivity conditions. Blood components and the chemical entities that they transport change predominantly with sex and age. The aim of this study was to utilize blood chemistry monitoring to establish the reference levels in a small prosimian primate, the Grey Mouse Lemur (Microcebus murinus). Method In the captive colony, mouse lemurs may live 10–12 years, and three age groups for both males and females were studied: young (1–3 years), middle-aged (4–5 years) and old (6–10 years). Blood biochemical markers were measured using the VetScan Comprehensive Diagnostic Profile. Because many life history traits of this primate are highly dependent on the photoperiod (body mass and reproduction), the effect of season was also assessed. Results The main effect of age was observed in blood markers of renal functions such as creatinine, which was higher among females. Additionally, blood urea nitrogen significantly increased with age and is potentially linked to chronic renal insufficiency, which has been described in captive mouse lemurs. The results demonstrated significant effects related to season, especially in blood protein levels and glucose rates; these effects were observed regardless of gender or age and were likely due to seasonal variations in food intake, which is very marked in this species. Conclusion These results were highly similar with those obtained in other primate species and can serve as references for future research of the Grey Mouse Lemur. PMID:23131178

2012-01-01

91

Effect of Withania somnifera Extracts on Some Selective Biochemical, Hematological, and Immunological Parameters in Guinea Pigs Experimental Infected with E. coli  

PubMed Central

Fifty 1-2-month-old Guinea pigs were divided into 5 equal groups, 10 each. Control (Gp1) did receive neither viable bacteria nor treatment. Each animal from the other groups (Gp2–5) was challenged with (1-2 × 108) viable E. coli in 200??L normal saline (0.9%) through IP route. GP2 infected group was treated with 200??L saline IP and kept as positive control group. Gp3-4 are infected and treated with Withania somnifera (ethanol root extract) with doses 50 and 100?mg/kg. BW, respectively. Gp5 infected treated group was treated with cefoperazone antibiotic at dose 35?mg/Kg BW. The treatment by drug or the extracted medicinal plant was started 72?h after infection for 7 successive days. Serum and whole blood sample were collected from all groups 14 days after treatment to evaluate some hematological and biochemical changes as well as immunomodulatory cytokine tumor necrosis factor-alpha (TNF-?). Oral treatment of the plant extract caused significant benefit results in infected Guinea pig appeared in the correction of some hematological and biochemical parameters also try to suppressed inflammatory cytokine response represent in TNF-?. It could be concluded that W. somnifera extract has potent antibacterial activity, and this appears in the correction with hematological, biochemical, and immunological results. PMID:23738137

El-Boshy, Mohamed El-Sayed; Abdalla, Osama Mohamed; Risha, Angy; Moustafa, Fatma

2013-01-01

92

Determining tumor blood flow parameters from dynamic image measurements  

NASA Astrophysics Data System (ADS)

Many recent cancer treatments focus on preventing angiogenesis, the process by which a tumor promotes the growth of large and efficient capillary beds for the increased nourishment required to support the tumor's rapid growth[l]. To measure the efficacy of these treatments in a timely fashion, there is an interest in using data from dynamic sequences of contrast-enhanced medical imaging, such as MRI and CT, to measure blood flow parameters such as perfusion, permeability-surface-area product, and the relative volumes of the plasma and extracellular-extravascular space. Starting with a two compartment model presented by the radiology community[2], this work challenges the application of a simplification to this problem, which was originally developed to model capillary reuptake[3]. While the primary result of this work is the demonstration of the inaccuracy of this simplification, the remainder of the paper is dedicated to presenting alternative methods for calculating the perfusion and plasma volume coefficients. These methods are applied to model data sets based on real patient data, and preliminary results are presented.

Libertini, Jessica M.

2008-11-01

93

Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats  

PubMed Central

Background Since ancient times, honey has been used for medicinal purposes in many cultures; it is one of the oldest and most enduring substances used in wound management. Scientific evidence for its efficacy is widely studied, but systemic safety studies are still lacking. It is essential to study the impact of consumption of honey on the health and proper development of the consumer. Therefore, the present study was designed to observe the effects of acute administration (14 days) of Gelam honey (GH), a wild harvesting honey and Acacia honey (AH), a beekeeping honey, on male and female Sprague Dawley (SD) rats. Methods An acute oral study was performed following OECD test guideline 423, with minor modifications. In the study, GH, AH and sucrose (S) were administered at 2000 mg/kg body weight. Animals were observed for the next 14 days. Gross pathology was performed at the end of the study. Animals were observed for mortality, morbidity, body weight changes, feed and water intake. Clinical biochemistry, gross pathology, relative organ weight and histopathological examination were performed. Results Rats fed with honey did not exhibit any abnormal signs or deaths. Results showed a decrease in weight gain and energy efficiency, but significantly increased in total food intake and total calories in female rats fed with GH, compared to control (p?biochemical parameters but in line with OECD regulation. Gelam honey may have potential in controlling weight gain and triglyceride levels in female rats compared to Acacia honey. SD rats have some effect on biochemical parameters, an exploration of which would make for intriguing analysis. PMID:24885010

2014-01-01

94

EFFECTS OF INHALATION OF SOLUBLE METALLIC CONSTITUENTS OF PARTICULATE MATTER ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN GUINEA PIGS  

EPA Science Inventory

EFFECTS OF INHALATION OF SOLUBLE METALLIC CONSTITUENTS OF PARTICULATE MATTER ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN GUINEA PIGS. JP Nolan1, LB Wichers2, J Stanek3, UP Kodavanti1, MCJ Schladweiler1, PA Evansky1, ER Lappi1, DL Costa1, and WP Watkinson1...

95

EFFECTS OF ACUTE EXPOSURE TO CONCENTRATED AMBIENT PARTICULATES ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN OLD SPONTANEOUSLY HYPERTENSIVE RATS  

EPA Science Inventory

EFFECTS OF ACUTE EXPOSURE TO CONCENTRATED AMBIENT PARTICULATES ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN OLD SPONTANEOUSLY HYPERTENSIVE RATS. JP Nolan1, LB Wichers2, DW Winsett1, UP Kodavanti1, MCJ Schladweiler1, DL Costa1, and WP Watkinson1. 1US E...

96

Long-term changes in microbial and biochemical parameters in the Central Indian Basin  

NASA Astrophysics Data System (ADS)

Natural kilometer-scale spatial variability and seasonal variability in deep-sea sediments at ˜5000 m depth was studied at two reference sites in the Central Indian Basin. Biochemical parameters such as the labile organic matter (LOM) (consisting of carbohydrates, protein and lipids) and total organic matter (TOM) and biological parameters such as total counts of bacteria and adenosine triphosphate (ATP) were estimated after an interval of 54 months. These were compared with nine locations (12-17 km away) where an artificial sediment disturbance was created by a hydraulic benthic disturber. Besides the above-mentioned parameters, extracellular enzymes, alkaline phosphatase, aminopeptidase and lipase were also monitored at these nine locations. Post-disturbance (PoD1) observations were carried out immediately after the benthic disturbance experiment and compared with the pre-disturbance (PreD) pattern. The restoration process in the test site was studied after a period of 44 months (PoD2). There was a drastic reduction in total counts of bacteria, ATP, carbohydrates and lipids accompanied by a two- to four-fold increase in protein content at the undisturbed reference sites after 54 months. A similar reduction in these parameters was observed in the PoD1 compared to the PreD stage at the test site. An initial increase in TOM content and in the activities of three extracellular enzymes in the PoD1 stage was followed by a reduction in TOM, ATP and organic P content and activities of the extracellular enzymes in the PoD2 phase (i.e. after 44 months). Reduction in ATP levels suggests a negative impact of resedimentation on living biomass in the sediments. Although LOM, protein concentrations and the protein/carbohydrate ratio increased in the PoD2 phase relative to the PoD1 phase, the bacterial numbers were below the concentrations in the PreD stage, indicating slow restoration of benthic conditions. Long-term analyses of the indicator parameters thus showed mixed effects of sediment disturbance. A similar pattern at the undisturbed reference location suggests that natural (seasonal) variability outweighed artificial variability caused by the benthic disturbance in the Central Indian Basin and the former is responsible for bringing about changes in deep-sea benthic ecosystem on long-time scales.

Raghukumar, Chandralata; Nagender Nath, B.; Sharma, R.; Loka Bharathi, P. A.; Dalal, S. G.

2006-10-01

97

A simple and feasible questionnaire to estimate menstrual blood loss: relationship with hematological and gynecological parameters in young women  

PubMed Central

Background Menstrual blood loss (MBL) has been shown to be an important determinant in iron status, work performance and well-being. Several methods have been developed to estimate MBL, the standard quantitative method however has limited application in clinical practice as it is expensive and requires women to collect, store and submit their sanitary products for analysis. We therefore aimed to develop a MBL-score based on a questionnaire, and to validate it by several hematological and biochemical parameters in women of childbearing age. Methods A total of 165 healthy young women were recruited. Hematological (hematocrit, hemoglobin, erythrocyte, leucocyte and platelet counts) and iron status (serum iron, serum ferritin, serum transferrin, and total iron binding capacity) parameters were analyzed at baseline. Women were asked to fulfill two gynecological questionnaires: a general questionnaire, to inform about the volunteer’s general menstrual characteristics; and a MBL questionnaire, to provide details of the duration of menstruation, number of heavy blood loss days, and number and type of pads and/or tampons used during the heaviest bleeding day, for all consecutive menstrual periods during 16 weeks. A MBL-score was calculated for each period and women, and its reliability determined by the Cronbach’s alpha coefficient. Pearson’s linear correlation tests were performed between blood parameters and the MBL-score. Two clusters were formed according the MBL-score (cluster 1: low MBL and cluster 2: high MBL). Results Significant higher MBL-score was observed in women who reported having a history of anemia (p?=?0.015), staining the bed at night during menstruation (p?blood loss (p?=?0.044), compared to those who did not. Women who used hormonal contraceptives presented lower MBL-scores than the others (p?=?0.004). The MBL-score was negatively associated with log-ferritin (p?=?0.006) and platelet count (p?=?0.011). Women in cluster 1 presented higher ferritin (p?=?0.043) than women in cluster 2. Conclusions We developed an easy and practical method for estimating menstrual blood loss based on a score calculated from a questionnaire in healthy women at childbearing age. The MBL-score is highly reliable and reflects menstrual blood loss validated by hematological and biochemical parameters. PMID:24886470

2014-01-01

98

Propolis alleviates aluminium-induced lipid peroxidation and biochemical parameters in male rats.  

PubMed

Aluminium is present in many manufactured foods and medicines and is also added to drinking water during purification purposes. Therefore, the present experiment was undertaken to determine the effectiveness of propolis in alleviating the toxicity of aluminium chloride (AlCl3) on biochemical parameters, antioxidant enzymes and lipid peroxidation of male Wistar Albino rats. Animals were assigned to 1 of 4 groups: control; 34 mg AlCl3/kg bw; 50 mg propolis/kg bw; AlCl3 (34 mg/kg bw) plus propolis (50 mg/kg bw), respectively. Rats were orally administered their respective doses daily for 70 days. The levels of thiobarbituric acid reactive substances (TBARS) was increased, and the activities of glutathione S-transferase, superoxide dismutase, catalase and glutathione peroxidase were decreased in liver, kidney and brain of rats treated with AlCl3. While, TBARS was decreased and the antioxidant enzymes were increased in rats treated with propolis alone. Plasma transaminases, lactate dehydrogenase, glucose, urea, creatinine, bilirubin, total lipid, cholesterol, triglyceride and LDL-c were increased, while total protein, albumin and high HDL-c were decreased due to AlCl3 administration. The presence of propolis with AlCl3 alleviated its toxic effects in rats treated with AlCl3. It can be concluded that propolis has beneficial influences and could be able to antagonize AlCl3 toxicity. PMID:19425229

Newairy, Al-Sayeda A; Salama, Afrah F; Hussien, Hend M; Yousef, Mokhtar I

2009-06-01

99

Effect of cytisine on some brain and hepatic biochemical parameters in spontaneously hypertensive rats  

PubMed Central

Tobacco smoking is a risk factor for variety of cardio-vascular diseases, such as hypertension, myocardial infarction, stroke and many others. It is of great importance for hypertensive patients to stop smoking. One of the medicines widely used for smoking cessation in Bulgaria is the original Bulgarian product Tabex®, which is developed on the basis of natural plant alkaloid cytisine. The aim of the following study was to ivestigate the effects of cytisine on some brain and hepatic biochemical parameters in spontaneously hypertensive rats (SHR), an widely used rodent model for human essential hypertension, and to compare the obtained results with their age-matched normotensive controls Wistar Kyoto (WKY). Multiple cytisine administration did not affect the activity of ethylmorphine-N-demethylase (EMND) and anylinehydroxylase (AH), as well as the quantity of cytochrome P 450, nor in WKY neither in SHR In the liver cytisine increased the MDA quantity both in SHR and in WKY, by 25% (p<0.05) and by 29% (p<0.05) respectively, while the GSH level was not significantly changed by the compound in both strains. In contrast, on the brain level, cytisine administration to SHR caused more prominent toxicity, resulted in GSH depletion and increased MDA quantity, while in WKY strain did not exert any toxicity. Cytisine did not significantly affect ALAT and ASAT activity in both strains. In conclusion, the results of our study suggest higher brain toxicity of cytisine in spontaneously hypertensive rats, that might be due to their pathophysiological characteristics. PMID:21217867

Simeonova, Rumyana; Vitcheva, Vessela; Mitcheva, Mitka

2010-01-01

100

Effect of cytisine on some brain and hepatic biochemical parameters in spontaneously hypertensive rats.  

PubMed

Tobacco smoking is a risk factor for variety of cardio-vascular diseases, such as hypertension, myocardial infarction, stroke and many others. It is of great importance for hypertensive patients to stop smoking. One of the medicines widely used for smoking cessation in Bulgaria is the original Bulgarian product Tabex®, which is developed on the basis of natural plant alkaloid cytisine. The aim of the following study was to ivestigate the effects of cytisine on some brain and hepatic biochemical parameters in spontaneously hypertensive rats (SHR), an widely used rodent model for human essential hypertension, and to compare the obtained results with their age-matched normotensive controls Wistar Kyoto (WKY). Multiple cytisine administration did not affect the activity of ethylmorphine-N-demethylase (EMND) and anylinehydroxylase (AH), as well as the quantity of cytochrome P 450, nor in WKY neither in SHR In the liver cytisine increased the MDA quantity both in SHR and in WKY, by 25% (p<0.05) and by 29% (p<0.05) respectively, while the GSH level was not significantly changed by the compound in both strains. In contrast, on the brain level, cytisine administration to SHR caused more prominent toxicity, resulted in GSH depletion and increased MDA quantity, while in WKY strain did not exert any toxicity. Cytisine did not significantly affect ALAT and ASAT activity in both strains. In conclusion, the results of our study suggest higher brain toxicity of cytisine in spontaneously hypertensive rats, that might be due to their pathophysiological characteristics. PMID:21217867

Simeonova, Rumyana; Vitcheva, Vessela; Mitcheva, Mitka

2010-03-01

101

Changes in biochemical, strength, flexibility, and aerobic capacity parameters after a 1700 km ultraendurance cycling race.  

PubMed

The purpose of the present research was to study the organic response after ultraendurance cycling race. Selected biochemical, leg strength, flexibility, and aerobic capacity parameters were analyzed in 6 subjects 5 days before and 5 days after completing a 1700?km ultraendurance cycling race. After the race, participants presented a significant decrease in Hb (167.8 ± 9.5 versus 141.6 ± 15.7?mg/dL), strength (29.4 ± 2.7 versus 25.5 ± 3.7?cm in a countermovement jump), and oxygen uptake and heart rate at ventilatory threshold (1957.0 ± 458.4 versus 1755.2 ± 281.5?mL/kg/min and 140.0 ± 9.7 versus 130.8 ± 8.3?bpm, resp.). Testosterone presented a decrease tendency (4.2 ± 2.5 versus 3.9 ± 2.6?ng/L) in opposition to the increase tendency of cortisol and ammonium parameters. Transferrin and iron levels presented high values related to an overstimulation of the liver, a normal renal function, a tendency to decrease flexibility, and an increase in aerobic capacity, finding a tendency to increase the absolute maximal oxygen uptake (37.2 ±2.4 versus 38.7 ± 1.8?mL/min) in contrast to previous studies conducted with subjects with similar age. These results can be used to program training interventions, recovery times between probes, and nutritional and/or ergonomic strategies in ultraendurance events. PMID:25180188

Clemente-Suarez, Vicente Javier

2014-01-01

102

Correlation Analysis between Complex Relative Permittivity and Biochemical Components for Blood of Dialysis Patients before and after Hemodialysis  

NASA Astrophysics Data System (ADS)

We previously measured for healthy subjects and patients who require hemodialysis the complex relative permittivity (?r´-j?r´´) of whole blood, and found that ?r´-axis intercept ?rt´ of straight line approximation to the Cole-Cole plots at frequencies from 200 MHz to 1GHz is significantly different with a level of less than 1% among its averaged values for healthy subjects and patients before and after dialysis, though any correlations between ?rt´ and blood urea nitrogen (BUN) and creatinine being used as main indices for hemodialysis evaluation have not so far been clarified. In this study, to make correlation analyses between the intercept ?rt´ and blood biochemical components for nine patients before and after hemodialysis, we measured six kinds of their blood components including BUN and creatinine, and calculated their Pearson product-moment correlation coefficients for ?rt´ along with significant probability P based on a t-test. It should be noted that P is a reference probability to determine whether or not a null hypothesis can be rejected, and that the P value of 0.05 is commonly used as a significance level for statistical test. As a result, we found that a strong correlation with P < 0.05 is observed between ?rt´ and albumin, while there are not always significant correlations with P > 0.05 between ?rt´ and other blood components including BUN and creatinine. Although albumin is not used as an index for hemodialysis efficiency, it can reflect water amount in blood vessels and liver operation. This finding implies that ?rt´ could be used as an index for evaluating blood viscosity and liver function.

Takeda, Akira; Takata, Kazuyuki; Nagao, Hirotomo; Wang, Jianqing; Fujiwara, Osamu

103

Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna.  

PubMed

Ectoine (ECT) is a compatible solute produced by soil, marine and freshwater bacteria in response to stressful factors. The purpose of our study was to determine the possible toxic influence of ECT on Daphnia magna. We determined the following endpoints: survival rate during exposure and recovery, swimming performance, heart rate, thoracic limb movement determined by image analysis, haemoglobin level by ELISA assay, catalase and nitric oxide species (NOx) by spectrophotometric methods. The results showed 80% survival of daphnids exposed to 50mg/L of ECT after 24h and 10% after 90h, however lower concentrations of ECT were well tolerated. A concentration-dependent reduction of swimming velocity was noted at 24 and 48h of the exposure. ECT (at 2.5 and 4mg/L) induced an increase of heart rate and thoracic limb movement (at 2.5, 4 and 20mg/L) after 24h. After 10h of the exposure to ECT daphnids showed a concentration-dependent increase of haemoglobin level synthesized and accumulated in the epipodite epithelia. After 24h we noted a concentration-dependent decrease of haemoglobin level and its lowest value was found after 48h of the exposure. ECT at a concentration of 20 and 25mg/L slightly stimulated catalase activity after 24h. NOx level was also increased after 10h of the exposure to 20 and 25mg/L of ECT reaching maximal activity after 24h. Our results suggest that ECT possesses some modulatory potential on the behaviour, physiology and biochemical parameters in daphnids. PMID:25460046

Bownik, Adam; St?pniewska, Zofia; Skowro?ski, Tadeusz

2015-02-01

104

An Enzyme Cascade in the Blood Clotting Mechanism, and its Function as a Biochemical Amplifier  

Microsoft Academic Search

AFTER years of confusion, it seems that a relatively simple pattern is emerging from present theories of blood coagulation. Its recognition is assisted by the Roman numeral terminology of the International Committee on Blood Clotting Factors, which, by displacing a profusion of synonyms, allows the basis of factual agreement to be seen. Physiological clotting seems to be initiated by contact

R. G. Macfarlane

1964-01-01

105

Studies of the biochemical regulation of blood cell formation. Final report, January 1-September 30, 1980  

SciTech Connect

This final report summarizes our most recent experimentation on the mechanism by which the erythropoietin interaction with cell surface receptors initiates nuclear RNA synthesis. It also describes the biological and biochemical effects of endotoxin-free erythropoietin, the finding that maximal hemoglobin synthesis in semi-solid medium probably requires cell-cell interactions and the initial clinical testing of erythropoietin in patients with anemia due to chronic renal disease.

Goldwasser, E.

1980-09-01

106

Interacting effects of diet and environmental temperature on biochemical parameters in the liver of Leuciscus idus melanotus (Cyprinidae: Teleostei)  

Microsoft Academic Search

One year old golden ide (Leuciscus idus melanotus) were starved for 2 weeks at 20°C (time zero) and then they were fed either freeze-dried mosquito larvae (natural diet) or a commercial fish chow (artificial diet) at an ambient temperature of 14°C and 20°C, respectively. Growth parameters and biochemical data in the liver were measured at time zero and after 3

Johannes Rafael I; Thomas Braunbeck

1988-01-01

107

Effect of Ocimum sanctum (Tulsi) and vitamin E on biochemical parameters and retinopathy in streptozotocin induced diabetic rats  

Microsoft Academic Search

This study was carried out to see the effect of the aqueous extract ofOcitum sanctum Linn (Tulsi) with Vitamin E on biochemical parameters and retinopathy in the streptozotocin-induced diabetic albino male\\u000a rats. Adult albino male rats weighing 150–200 gm were made diabetic by intraperitoneal injection of streptozotocin in the\\u000a dose 60 mg\\/kg in citrate buffer (pH 6.3). The diabetic animals

Eshrat M. Halim; A. K. Mukhopadhyay

2006-01-01

108

Effects of rearing temperature on hematological and biochemical parameters of great sturgeon ( Huso huso Linnaeus, 1758) juvenile  

Microsoft Academic Search

The effect of environmental temperature changes on hematological and biochemical parameters of Huso huso juveniles was studied. Six-month-old juveniles with mean body weight of 69.2?±?4.1 g were subjected to different temperatures\\u000a (9–14°C, 15–20°C, and 21–26°C, respectively). The hematological parameters, ion Ca2+, glucose, and the cortisol concentrations\\u000a were assessed after a period of 21 days rearing at these temperatures. The results show that

Asad Mohammadi Zarejabad; Mohammad Sudagar; Somayeh Pouralimotlagh; Kazem Darvish Bastami

2010-01-01

109

High-Throughput Immunoassay for the Biochemical Diagnosis of Friedreich Ataxia in Dried Blood Spots and Whole Blood  

PubMed Central

BACKGROUND Friedreich ataxia (FRDA) is caused by reduced frataxin (FXN) concentrations. A clinical diagnosis is typically confirmed by DNA-based assays for GAA-repeat expansions or mutations in the FXN (frataxin) gene; however, these assays are not applicable to therapeutic monitoring and population screening. To facilitate the diagnosis and monitoring of FRDA patients, we developed an immunoassay for measuring FXN. METHODS Antibody pairs were used to capture FXN and an internal control protein, ceruloplasmin (CP), in 15 ?L of whole blood (WB) or one 3-mm punch of a dried blood spot (DBS). Samples were assayed on a Luminex LX200 analyzer and validated according to standard criteria. RESULTS The mean recovery of FXN from WB and DBS samples was 99%. Intraassay and interassay imprecision (CV) values were 4.9%–13% and 9.8%–16%, respectively. The FXN limit of detection was 0.07 ng/mL, and the reportable range of concentrations was 2–200 ng/mL. Reference adult and pediatric FXN concentrations ranged from 15 to 82 ng/mL (median, 33 ng/mL) for DBS and WB. The FXN concentration range was 12–22 ng/mL (median, 15 ng/mL) for FRDA carriers and 1–26 ng/mL (median 5 ng/mL) for FRDA patients. Measurement of the FXN/CP ratio increased the ability to distinguish between patients, carriers, and the reference population. CONCLUSIONS This assay is applicable to the diagnosis and therapeutic monitoring of FRDA. This assay can measure FXN and the control protein CP in both WB and DBS specimens with minimal sample requirements, creating the potential for high-throughput population screening of FRDA. PMID:23838345

Oglesbee, Devin; Kroll, Charles; Gakh, Oleksandr; Deutsch, Eric C.; Lynch, David R.; Gavrilova, Ralitza; Tortorelli, Silvia; Raymond, Kimiyo; Gavrilov, Dimitar; Rinaldo, Piero; Matern, Dietrich; Isaya, Grazia

2014-01-01

110

A method for assessment of blood volume parameters in pregnant sheep using fluorescein-labelled dextran.  

PubMed

The assessment of blood volume parameters in clinical and research settings has been limited by methods that involve radioactivity, complex assays or are unreliable. We aimed to design a method for measuring blood volume parameters that was non-radioactive, simple, cheap and reliable. We have used a commercially available fluorescein-labelled 250kDa dextran, a large inert molecule, and have measured dilution of this through the intravascular space of pregnant ewes. From this estimation of plasma volume and measured hematocrit, we have calculated blood volume and red cell volume. The blood volume results are 6% lower than those obtained using radiolabelled red cells, but there is no significant difference in red cell volume between methods. The coefficient of variation for repeated measurements of plasma volume measurements is 3.8%. This is a simple, reliable, cheap and non-radioactive method for estimating blood volume parameters in pregnant sheep, and may prove useful in other settings. PMID:17953987

Rumball, C W H; Van Zijl, P; Rutland, M D; Bloomfield, F H; Harding, J E

2008-01-01

111

Effects of cadmium at sub-lethal concentration on growth and biochemical parameters in rainbow trout (Oncorhynchus mykiss)  

PubMed Central

Cadmium (Cd), as one of heavy metals and an environmental stressor, may alter many physiological processes like growth and serum parameters in fish. The main objective of this study was to determine the effects of cadmium at sub-lethal concentrations (1 and 3 ?g/l) on growth and serum biochemical parameters including enzymes, i.e. alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), glucose, triglyceride, cholesterol and total protein in rainbow trout (Oncorhynchus mykiss). Trout were exposed to cadmium, and, at intervals of 1, 15, and 30 days, selected parameters were evaluated. Condition Factor (K), Specific Growth Rate (SGR) and Body Weight Gain (BWG) consistently decreased, while Food Conversion Ratio (FCR) increased at the end of experiment. Glucose was elevated in trout exposed to both Cd concentrations at day 15 and then returned to levels comparable to control fish. Triglyceride and cholesterol decreased transiently at day 15 and then increased at day 30. Total protein, AST, ALT and ALP increased linearly by time and Cd concentration. This investigation suggests that growth and serum biochemical parameters could be used as important and sensitive biomarkers in ecotoxicological studies concerning the effects of metal contamination and fish health. PMID:23782857

2013-01-01

112

Biochemical and Molecular Aspects of Vascular Adrenergic Regulation of Blood Pressure in the Elderly  

PubMed Central

Hypertension, orthostatic hypotension, arterial insufficiency, and atherosclerosis are common disorders in the elderly that lead to significant morbidity and mortality. One common factor to these conditions is an age-related decline in vascular beta-adrenergic receptor-mediated function and subsequent cAMP generation. Presently, there is no single cellular factor that can explain this age-related decline, and thus, the primary cause of this homeostatic imbalance is yet to be identified. However, the etiology is clearly associated with an age-related change in the ability of beta-adrenergic receptor to respond to agonist at the cellular level in the vasculature. This paper will review what is presently understood regarding the molecular and biochemical basis of age-impaired beta-adrenergic receptor-mediated signaling. A fundamental understanding of why ?-AR-mediated vasorelaxation is impaired with age will provide new insights and innovative strategies for the management of multiple clinical disorders. PMID:21961055

Schutzer, William E.; Mader, Scott L.

2012-01-01

113

Neonatal Meningitis: What Is the Correlation Among Cerebrospinal Fluid Cultures, Blood Cultures, and Cerebrospinal Fluid Parameters?  

Microsoft Academic Search

BACKGROUND.Meningitis is a substantial cause of morbidity and mortality in neo- nates. Clinicians frequently use the presence of positive blood cultures to deter- mine whether neonates should undergo lumbar puncture. Abnormal cerebrospi- nal fluid (CSF) parameters are often used to predict neonatal meningitis and determine length and type of antibiotic therapy in neonates with a positive blood culture and negative

Harmony P. Garges; M. Anthony Moody; C. Michael Cotten; P. Brian Smith; Kenneth F. Tiffany; Robert Lenfestey; Jennifer S. Li; Vance G. Fowler

2010-01-01

114

Copper bioavailability, blood parameters, and nutrient balance in mink.  

PubMed

A 3 × 3 + 1 factorial experiment was conducted based on a completely randomized design to evaluate the effects of different sources of copper on plasma metabolites, nutrient digestibility, relative copper bioavailability, and retention of some minerals in male mink. Animals in the control group were fed a basal diet, which mainly consisted of corn, fish meal, meat and bone meal, and soybean oil, with no copper supplementation. Mink in the other 9 treatments were fed the basal diet supplemented with Cu from reagent-grade copper sulfate (CuSO4), tribasic copper chloride (TBCC), or copper methionine (CuMet). Copper concentrations of the experimental diets were 50, 100, and 150 mg Cu/kg DM. Blood samples were collected via the toe clip at the end of study (d 42) to determine blood hematology and blood metabolites. A metabolism trial of 4 d was conducted during the last week of experimental feeding. There was a linear (P < 0.01) effect of dose of Cu on plasma Cu concentrations, ceruloplasmin concentration, and Cu-Zn superoxide dismutase activity. A linear response to Cu dose was noted for fat (P < 0.05) digestibility. Supplemental dose of Cu linearly increased (P < 0.05) liver Cu and decreased (P < 0.05) liver Zn level but did not alter liver Fe. The concentration of liver Cu of the mink fed with TBCC and CuMet diets was greater (P < 0.05) than that fed CuSO4. Compared with CuSO4 (100%), relative bioavailability values of TBCC were 104 and 104%, based on serum ceruloplasmin and liver copper, respectively, and relative bioavailability values of CuMet were 130 and 111%. CuMet and TBCC are more bioavailable than CuSO4. In conclusion, the relative bioavailability of CuMet obtained in this study was greater than that of CuSO4 and TBCC. Dose of Cu had an important effect on the regulating ceruloplasmin concentration, Cu-Zn superoxide dismutase activity, and the digestion of dietary fat in mink. PMID:25568366

Wu, X Z; Zhang, T T; Guo, J G; Liu, Z; Yang, F H; Gao, X H

2015-01-01

115

Influence of different dietary levels of zinc on performance, vitamin B12, and blood parameters in lambs.  

PubMed

This experiment was conducted to investigate the influence of different dietary levels of zinc (Zn) on performance, vitamin B12, and blood parameters in lambs. Thirty six cannulated Poll Dorset x Small-tailed Han wether lambs were assigned randomly to four treatment groups: The control group, which was supplemented with 0.30 mg Co/kg dry matter (DM) to the basal diet; and the low-, medium- and high-Zn supplemented groups, supplementation of 50, 100, and 150 mg Zn/kg DM to the control diet, respectively. Lambs were housed in individual pens and the experiment lasted for 70 days. There was no significant difference in body weight gain and feed/gain between different treatment groups. The high-Zn supplemented lambs showed lower vitamin B12 concentrations in both ruminal fluid and plasma, and higher methylmalonic acid and homocysteine concentrations in plasma compared with the control and low-Zn supplemented groups (p < 0.05). No significant difference was observed in these biochemical values between the control, the low-, and the medium-Zn supplemented groups (p > 0.05). Plasma folate concentration, glucose, and heme-dependent blood parameters were not influenced by dietary zinc level. It was concluded that the higher level of zinc supplementation had a negative effect on vitamin B12 in lambs. Higher inclusion level of dietary zinc could inhibit vitamin B12 synthesis in the rumen of lambs. PMID:17607954

Wang, Runlian; Zhu, Xiaoping; Guo, Fucun; Zhang, Wei; Jia, Zhihai

2006-11-01

116

A Method for Assessment of Blood Volume Parameters in Pregnant Sheep using Fluorescein-labelled Dextran  

Microsoft Academic Search

The assessment of blood volume parameters in clinical and research settings has been limited by methods that involve radioactivity, complex assays or are unreliable. We aimed to design a method for measuring blood volume parameters that was non-radioactive, simple, cheap and reliable. We have used a commercially available fluorescein-labelled 250kDa dextran, a large inert molecule, and have measured dilution of

C. W. H. Rumball; P. Van Zijl; M. D. Rutland; F. H. Bloomfield; J. E. Harding

2008-01-01

117

Effects of chitin and sepia ink hybrid hemostatic sponge on the blood parameters of mice.  

PubMed

Chitin and sepia ink hybrid hemostatic sponge (CTSH sponge), a new biomedical material, was extensively studied for its bene?cial biological properties of hemostasis and stimulation of healing. However, studies examining the safety of CTSH sponge in the blood system are lacking. This experiment aimed to examine whether CTSH sponge has negative effect on blood systems of mice, which were treated with a dosage of CTSH sponge (135 mg/kg) through a laparotomy. CTSH sponge was implanted into the abdominal subcutaneous and a laparotomy was used for blood sampling from abdominal aortic. Several kinds of blood parameters were detected at different time points, which were reflected by coagulation parameters including thrombin time (TT), prothrombin time (PT), activated partial thromboplatin time (APTT), fibrinogen (FIB) and platelet factor 4 (PF4); anticoagulation parameter including antithrombin III (AT-III); fibrinolytic parameters including plasminogen (PLG), fibrin degradation product (FDP) and D-dimer; hemorheology parameters including blood viscosity (BV) and plasma viscosity (PV). Results showed that CTSH sponge has no significant effect on the blood parameters of mice. The data suggested that CTSH sponge can be applied in the ?eld of biomedical materials and has potential possibility to be developed into clinical drugs of hemostatic agents. PMID:24727395

Zhang, Wei; Sun, Yu-Lin; Chen, Dao-Hai

2014-04-01

118

Effects of Chitin and Sepia Ink Hybrid Hemostatic Sponge on the Blood Parameters of Mice  

PubMed Central

Chitin and sepia ink hybrid hemostatic sponge (CTSH sponge), a new biomedical material, was extensively studied for its bene?cial biological properties of hemostasis and stimulation of healing. However, studies examining the safety of CTSH sponge in the blood system are lacking. This experiment aimed to examine whether CTSH sponge has negative effect on blood systems of mice, which were treated with a dosage of CTSH sponge (135 mg/kg) through a laparotomy. CTSH sponge was implanted into the abdominal subcutaneous and a laparotomy was used for blood sampling from abdominal aortic. Several kinds of blood parameters were detected at different time points, which were reflected by coagulation parameters including thrombin time (TT), prothrombin time (PT), activated partial thromboplatin time (APTT), fibrinogen (FIB) and platelet factor 4 (PF4); anticoagulation parameter including antithrombin III (AT-III); fibrinolytic parameters including plasminogen (PLG), fibrin degradation product (FDP) and D-dimer; hemorheology parameters including blood viscosity (BV) and plasma viscosity (PV). Results showed that CTSH sponge has no significant effect on the blood parameters of mice. The data suggested that CTSH sponge can be applied in the ?eld of biomedical materials and has potential possibility to be developed into clinical drugs of hemostatic agents. PMID:24727395

Zhang, Wei; Sun, Yu-Lin; Chen, Dao-Hai

2014-01-01

119

Changes in quality and biochemical parameters in 'Idared' apples during prolonged shelf life and 1-MCP treatment.  

PubMed

In this study, changes in quality and various biochemical parameters of 'Idared' apples during prolonged shelf life period after ultra-low oxygen (ULO) storage were investigated. Additionally, the impact of the postharvest application of 1-methylcyclopropene (1-MCP) on different parameters was evaluated. After the harvest, apples were stored in the ULO storage for 6 months and then exposed to room temperature. Fruit firmness, peel color, and changes in sugars, organic acids and phenolics were monitored during the 3 weeks of shelf life. Malic acid, sugars and firmness decreased at room temperature. However, the color of the apples remained unchanged. The level of citric and ascorbic acid remained constant. Levels of phenolics in the peel increased significantly, whereas remained constant in the pulp of apples. 1-MCP treatment resulted in higher amounts of fructose and glucose, malic acid and greater firmness of apples. However, 1-MCP did not influence the phenolic content, ascorbic acid or color. The results obtained indicate that the content of different health-promoting compounds of apples does not change dramatically at room temperature. At the same time these results suggest that 1-MCP could be useful for maintaining certain quality and biochemical parameters and might extend the shelf life of apples. PMID:23175783

Bizjak, Jan; Slatnar, Ana; Stampar, Franci; Veberic, Robert

2012-12-01

120

[Evaluation of dietary therapy efficiency, based on clinical and biochemical parameters in patients with cardiovascular disease and osteopenia].  

PubMed

Aim of investigation was to analysis of dietary intake in patients with cardiovascular diseases (CVD) and osteopenia. We studied 13 patients with CDV and osteopenia and 117 control subjects with CDV. Analysis of dietary intake in all patients showed high level consumption of fat, excessive consumption of phosphorus and deficiency in consumption of vitamins A, B1, B2. It was shown that diet-therapy has promoted the most positive changes of the clinical, biochemical and immunological parameters of patients had osteopenia less then control group. PMID:18030809

Gapparova, K M; Pogozheva, A V; Baturin, A K; Ogloblin, N A; Derbeneva, S A; Mal'tsev, G Iu; Trushina, E N; Mustafina, O K

2007-01-01

121

Effect of hafnium and titanium coated implants on several blood biochemical markers after osteosynthesis in rabbits  

PubMed Central

Purpose: An experimental study comparing the dynamics of several biochemical markers before and after osteosynthesis, utilizing implants coated with titanium and hafnium nitrides and non-coated implants on rabbits’ bones. Materials and methods: The Study has been conducted on 30 rabbits of both sexes, at the age of 6-7 months, weighing 2526.5±74.4 gm. Animals underwent open osteotomy of the tibia in the middle third of the diaphysis followed by the intramedullary nailing. The level of alkaline phosphatase, calcium, phosphorus, total protein, glucose, ALT and AST were monitored for 60 days. Results: the use of implants coated with titanium and hafnium nitrides, which have high strength, thermal and chemical stability, was not accompanied by the development of additional negative reactive changes compared to non-coated implants. Conclusion: Nanotechnology used in manufacturing bioinert coatings for implants for osteosynthesis, has made the post-operative period less complicated as reflected by less expressed changing in the markers of bone metabolism and hepatotoxicity. PMID:25419385

Yousef, Ashraf; Akhtyamov, Ildar; Shakirova, Faina; Zubairova, Lyaili; Gatina, Elmira; Aliev, ?lchin

2014-01-01

122

Interaction of lipophorin with Rhodnius prolixus oocytes: biochemical properties and the importance of blood feeding  

PubMed Central

Lipophorin (Lp) is the main haemolymphatic lipoprotein in insects and transports lipids between different organs. In adult females, lipophorin delivers lipids to growing oocytes. In this study, the interaction of this lipoprotein with the ovaries of Rhodnius prolixus was characterised using an oocyte membrane preparation and purified radiolabelled Lp (125I-Lp). Lp-specific binding to the oocyte membrane reached equilibrium after 40-60 min and when 125I-Lp was incubated with increasing amounts of membrane protein, corresponding increases in Lp binding were observed. The specific binding of Lp to the membrane preparation was a saturable process, with a Kdof 7.1 ± 0.9 x 10-8M and a maximal binding capacity of 430 ± 40 ng 125I-Lp/µg of membrane protein. The binding was calcium independent and pH sensitive, reaching its maximum at pH 5.2-5.7. Suramin inhibited the binding interaction between Lp and the oocyte membranes, which was completely abolished at 0.5 mM suramin. The oocyte membrane preparation from R. prolixus also showed binding to Lp from Manduca sexta. When Lp was fluorescently labelled and injected into vitellogenic females, the level of Lp-oocyte binding was much higher in females that were fed whole blood than in those fed blood plasma. PMID:24037104

Entringer, Petter Franco; Grillo, Luciano Aparecido Meireles; Pontes, Emerson Guedes; Machado, Ednildo Alcântara; Gondim, Katia Calp

2013-01-01

123

BIOCHEMICAL CHANGES IN HUMANS UPON EXPOSURE TO NITROGEN DIOXIDE WHILE AT REST  

EPA Science Inventory

The biochemical response to controlled exposures of nitrogen dioxide (NO2) was studied in 19 human subjects exposed to 0.2 ppm NO2 for 2 hr and compared to 15 control subjects exposed to filtered air for 2 hr. Seven biochemical blood parameters, including glutathione, red blood c...

124

Hematologic and biochemical reference intervals for Spanish ibex.  

PubMed

We studied blood samples from 529 Spanish ibexes (Capra pyrenaica) from different Andalusian mountain ranges in southern Spain, primarily from Sierra Nevada. For each sample, 13 hematologic and 32 biochemical parameters were analyzed. Within this database, we selected values obtained from live, free-ranging, physically restrained, clinically normal animals to determine reference intervals for these parameters. Distribution of values within each parameter was determined and differences in values between sex and age classes also were determined. We found significant differences in eight biochemical parameters among male and female ibexes. Significant differences in values for 20 hematologic and biochemical parameters between age classes also were found. PMID:12685085

Pérez, Jesús M; González, Francisco J; Granados, José E; Pérez, M Carmen; Fandos, Paulino; Soriguer, Ramón C; Serrano, Emmanuel

2003-01-01

125

Biochemical and functional disturbances in red blood cells of herring gulls ingesting Prudhoe Bay crude oil.  

PubMed

Heinz body hemolytic anemia developed in Herring Gull (Larus argentatus) nestlings given oral doses of 10 ml of Prudhoe Bay crude oil per kilogram of body weight per day for 5 days. Associated disturbances in red blood cells were increased amounts of reduced glutathione (GSH), peroxidation of membrane lipids, an increase in membrane permeability, and a decrease in the oxygen-carrying capacity of cyanomethemoglobin-convertible hemoglobin. Among groups of gulls given different cumulative doses of oil over a 6-day period, significant covariance with dose and dependence on dose was demonstrated for packed cell volume, hemoglobin, and red cell GSH. Rapid defecation of oil by gulls indicated that the effective dose was substantially less than the administered dose. Pronounced damage to red cells occurred in some birds administered oil for only 2 days. These data imply that the toxic effects of ingested oil may contribute significantly to the morbidity and mortality of oil-contaminated birds. PMID:4049418

Leighton, F A; Lee, Y Z; Rahimtula, A D; O'Brien, P J; Peakall, D B

1985-10-01

126

Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows  

PubMed Central

The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at day 7 of the estrous cycle to start the SOV protocol, as well as on the day of embryo recovery (DER). The SOV group produced 7.5 ± 6.7 oocytes/embryos, of which 3.4 ± 4.7 were TE. Serum urea and E2 and uterine Glu, CK, LDH, TP, P4 and PGFM in the control group and serum P4 and PGFM and uterine LDH and PGFM in the SOV group were significantly higher (p < 0.01) at DER than day 7. At DER, uterine urea, LDH, PGFM and TP and serum urea, LDH, PGFM, and P4 concentrations were higher (p < 0.01) in the SOV group than the control. There was no significant variation in uterine bacteriology or cytology. Overall, these results infer that SOV affects both serum profile and uterine secretions, and that these changes may influence the number of TE. PMID:24675828

Rasolomboahanginjatovo, Hasina Santatriniaina; Dupras, Raynald; Mills, Louis; Lefebvre, Réjean

2014-01-01

127

Genetic and phenotypic relationships between blood gas parameters and ascites-related traits in broilers.  

PubMed

Ascites, also called pulmonary hypertension syndrome, is a metabolic disorder in chickens that have an insufficient pulmonary vascular capacity. The tendency of broilers to develop ascites is heritable, and successful selection against this susceptibility would benefit from good and easy-to-measure indicator traits. Blood gas parameters have been suggested as indicator traits for ascites susceptibility. Therefore, the aim of the present study was to estimate the heritability of blood gas parameters and the genetic and phenotypic correlations between blood gas parameters, heart ratio (postmortem indicator for ascites), and BW at 2 different ages. For this purpose, blood gas parameters, including the partial pressure of carbon dioxide in venous blood (pvCO(2)), the partial pressure of oxygen in venous blood (pvO(2)), and blood oxygen saturation, were measured at an average age of 22 d in nearly 3,000 broilers. To challenge the resistance of the birds to ascites, they were kept under cold conditions. Heritability for heart ratio was 0.43, and the heritability estimates were low: 0.02 for pvCO(2), 0.03 for pvO(2), and 0.07 for blood oxygen saturation. The estimated heritability for pH was 0.15, for bicarbonate was 0.19, and for total carbon dioxide content was 0.19. The genetic correlations between heart ratio and total carbon dioxide content (0.31 +/- 0.15) and between heart ratio and bicarbonate (0.31 +/- 0.15) were moderate and positive. For pvO(2), the genetic correlation with heart ratio was stronger and negative (-0.62 +/- 0.21); however, this correlation could not be estimated accurately because of the low heritability of pvO(2). For pvCO(2), the genetic correlation with the heart ratio was close to zero (-0.04 +/- 0.45). Phenotypic correlations between traits were, in general, similar to the genetic correlations. Heritabilities for blood gas parameters and the genetic correlations between blood gas parameters and the heart ratio estimated in the present study do not support the suggestion that blood gas parameters measured during wk 3 or 4 are useful traits to select against the susceptibility for ascites. PMID:19211516

Closter, A M; van As, P; Groenen, M A M; Vereijken, A L J; van Arendonk, J A M; Bovenhuis, H

2009-03-01

128

Dynamics of microbial communities related to biochemical parameters during vermicomposting and maturation of agroindustrial lignocellulose wastes.  

PubMed

Scarce information is available on the changes in abundance of microbial taxa during vermicomposting. Quantitative PCR and DGGE analysis were used to monitor variations in the microbial structure, relative abundance of four bacterial classes and fungi over the vermicomposting and maturation period of wet olive cake (O) and vine shoots (W). Multivariate correlation analysis between microbial structure and abundance, earthworm biomass and enzyme activities revealed similar and divergent interactions in both processes. Although Eisenia fetida development was different, significant correlations were found with ?-glucosidase activity and with bacterial and fungal structure. In the vermicomposting period of O and W, a decline was found in bacteria (94% and 77%), fungi (93% and 94%), and Gammaproteobacteria (56% and 71%) but an increase in Betaproteobacteria and Actinobacteria (62-79%). Alphaproteobacteria increased only in O (26%). Despite the different initial lignocellulose wastes, the mature vermicomposts were similar in microbial and biochemical properties. PMID:23948272

Castillo, Jean Manuel; Romero, Esperanza; Nogales, Rogelio

2013-10-01

129

The effect of Antithrombin-III on routine hematological and biochemical parameters in an experimental animal model of skeletal muscle ischemia-reperfusion injury  

PubMed Central

Background: Antithrombin III (AT-III) has been shown to attenuate the local and systemic harmful effects of skeletal muscle ischemia-reperfusion (I-R) injury. The aim of the present study was to monitor the fluctuation of routine hematological and biochemical parameters in an experimental animal model of tourniquet-induced skeletal muscle I-R injury and to investigate how these are influenced by the protective administration of AT-III. Methods: Sixty male Wistar rats were submitted to a 6-hour, tourniquet-induced, complete ischemia of the right hind-limb. Animals were divided into those receiving AT-III (dose, 250 IU/kg) 30 minutes before the reperfusion (group A, n=30) and those receiving placebo (group B, n =30). Another 10 animals were sham-operated (group C). White blood cell (WBC) and platelet (PLT) count, aspartate and alanine aminotransferases (AST and ALT), alkaline phosphatase (ALP), and ?-glutamyl transferase (?-GT) were estimated in blood samples taken from the inferior vena cava at 3 different time points post-reperfusion (at baseline, at 30 minutes and at 4 hours) and groups A and B were compared using the Mann-Whitney U test. Results: There were no statistically significant differences between the AT-III and the placebo groups at 0, 30 minutes and 4 hours with regard to the WBC, ALT and ?-GT levels, however, there was a significant decrease of AST levels 4 hours post-reperfusion in the AT-III group compared to the placebo group (p=0.002). An increased PLT count and ALP levels 30 minutes post-reperfusion were also noted in the AT-III group compared to placebo (p<0.001; and p=0.001, respectively). Conclusions: Of the routine hematological and biochemical parameters tested, AST was found to be significantly suppressed at 4 hours in the AT-III-treated animals, suggesting a possible beneficial effect of AT-III in mouse skeletal muscle I-R injury. The effect of AT-III on PLTs and ALP levels merits further investigation. Hippokratia 2014; 18 (3): 234-239. PMID:25694758

Karamanos, DG; Karkos, CD; Kambaroudis, AG; Kritsepi, M; Papadopoulos, S; Spyridis, CT; Gerassimidis, TS

2014-01-01

130

Effects of Dietary L-carnitine Supplementation on Growth Performance, Organ Weight, Biochemical Parameters and Ascites Susceptibility in Broilers Reared Under Low-temperature Environment  

PubMed Central

The objective of this study was to investigate the effects of L-carnitine on growth performance, organ weight, biochemical parameters of blood, heart and liver, and ascites susceptibility of broilers at different ages reared under a low-temperature environment. A total of 420 1-d-old male Ross 308 broilers were randomly assigned to two dietary treatments with fifteen replicates of fourteen broilers each. Treatment diets consisted of L-carnitine supplementation at levels of 0 and 100 mg/kg. At 11-d of age, low temperature stress was used to increase ascites susceptibility. Blood, heart and liver samples were collected at different ages for analysis of boichemical parameters. The results showed that, there was no significant difference in growth performance with L-carnitine supplementation, but the mortality due to ascites was significantly decreased. Dietary L-carnitine supplementation significantly reduced heart index (HI) and ascites heart index (AHI) on d 21, lung index (LUI) on d 35 and liver index (LI) on d 42. The broilers fed diets containing L-carnitine had significantly lower red blood cell counts (RBC), hemoglobin (HGB) concentration and hematocrit (HCT) on d 42. Dietary L-carnitine supplementation significantly reduced malondialdehyde (MDA) content of heart tissue on d 21 and 35, and significantly increased total superoxide dismutase (T-SOD) and Glutathione peroxidase (GSH-Px) activity of the heart on d 21 and 42. L-carnitine supplementation significantly reduced serum triglyceride (TG) content on d 28 and 35 and serum glucose (GLU) on d 35 and 42, and significantly increased serum total protein (TP) and globulin (GLO) content on d 42. L-carnitine supplementation significantly enhanced liver succinodehydrogenase (SDH), malic dehydrogenase (MDH) and Na+-K+-ATPase activity on d 28, and tended to reduce the lactic acid (LD) level of liver on d 35 (p = 0.06). L-carnitine supplementation significantly reduced serum uric acid (UA) content on d 28, 35 and 42. Based on the current results, it can be concluded that dietary L-carnitine supplementation reduced organ index, red blood cell counts and hematocrit, enhanced antioxidative capacity of the heart, enhanced liver enzymes activity involved in tricarboxylic acid cycle, and reduced serum glucose and triglyceride. Therefore, it is suggested that L-carnitine can potentially reduce susceptibility and mortality due to ascites. PMID:25049781

Wang, Y. W.; Ning, D.; Peng, Y. Z.; Guo, Y. M.

2013-01-01

131

Training-level induced changes in blood parameters response to on-water rowing races  

Microsoft Academic Search

The study investigated blood markers allowing discriminating physiological responses to on-water rowing races, notably re- garding training volume of athletes and race duration. College (COL) and national (NAT) rowers performed a 1000- or 2000-m race. Capillary blood samples obtained before and post-race allowed an analysis of a wide range of serum parameters. COL rowers had a lower rowing experience and

François Denis Desgorces; Marc Testa; Cyril Petibois

132

Disturbances of morphological parameters in blood of rats orally exposed to aluminum chloride  

Microsoft Academic Search

The aim of this work was to assess changes of morphological parameters in the blood of rats after oral (po) administration\\u000a of aluminum (Al), in relation to the time and the administered dose. The experiment was performed on female Wistar rats. The\\u000a animals were administered aluminum chloride (100 mg Al\\/kg) daily for 21 d. Morphological assays: red blood cells (RBC),

Jadwiga Chmielnicka; Marzenna Nasiadek; Roman Pínkowski; Marek Paradowski

1994-01-01

133

Significant Biochemical, Biophysical and Metabolic Diversity in Circulating Human Cord Blood Reticulocytes  

PubMed Central

Background The transition from enucleated reticulocytes to mature normocytes is marked by substantial remodeling of the erythrocytic cytoplasm and membrane. Despite conspicuous changes, most studies describe the maturing reticulocyte as a homogenous erythropoietic cell type. While reticulocyte staging based on fluorescent RNA stains such as thiazole orange have been useful in a clinical setting; these ‘sub-vital’ stains may confound delicate studies on reticulocyte biology and may preclude their use in heamoparasite invasion studies. Design and Methods Here we use highly purified populations of reticulocytes isolated from cord blood, sorted by flow cytometry into four sequential subpopulations based on transferrin receptor (CD71) expression: CD71high, CD71medium, CD71low and CD71negative. Each of these subgroups was phenotyped in terms of their, morphology, membrane antigens, biomechanical properties and metabolomic profile. Results Superficially CD71high and CD71medium reticulocytes share a similar gross morphology (large and multilobular) when compared to the smaller, smooth and increasingly concave reticulocytes as seen in the in the CD71low and CD71negativesamples. However, between each of the four sample sets we observe significant decreases in shear modulus, cytoadhesive capacity, erythroid receptor expression (CD44, CD55, CD147, CD235R, and CD242) and metabolite concentrations. Interestingly increasing amounts of boric acid was found in the mature reticulocytes. Conclusions Reticulocyte maturation is a dynamic and continuous process, confounding efforts to rigidly classify them. Certainly this study does not offer an alternative classification strategy; instead we used a nondestructive sampling method to examine key phenotypic changes of in reticulocytes. Our study emphasizes a need to focus greater attention on reticulocyte biology. PMID:24116088

Malleret, Benoît; Xu, Fenggao; Mohandas, Narla; Suwanarusk, Rossarin; Chu, Cindy; Leite, Juliana A.; Low, Kayen; Turner, Claudia; Sriprawat, Kanlaya; Zhang, Rou; Bertrand, Olivier; Colin, Yves; Costa, Fabio T. M.; Ong, Choon Nam; Ng, Mah Lee; Lim, Chwee Teck; Nosten, Francois; Rénia, Laurent; Russell, Bruce

2013-01-01

134

Isolation and biochemical characterization of LEAP-2, a novel blood peptide expressed in the liver  

PubMed Central

The human genome contains numerous genes whose protein products are unknown in terms of structure, interaction partner, expression, and function. To unravel the function of these orphan genes, it is of particular value to isolate native forms of protein and peptide products derived from these genes. From human blood ultrafiltrate, we characterized a novel gene-encoded, cysteine-rich, and cationic peptide that we termed liver-expressed antimicrobial peptide 2 (LEAP-2). We identified several circulating forms of LEAP-2 differing in their amino-terminal length, all containing a core structure with two disulfide bonds formed by cysteine residues in relative 1–3 and 2–4 positions. Molecular cloning of the cDNA showed that LEAP-2 is synthesized as a 77-residue precursor, which is predominantly expressed in the liver and highly conserved among mammals. This makes it a unique peptide that does not exhibit similarity with any known human peptide regarding its primary structure, disulfide motif, and expression. Analysis of the LEAP-2 gene resulted in the identification of an alternative promoter and at least four different splicing variants, with the two dominating transcripts being tissue-specifically expressed. The largest native LEAP-2 form of 40 amino acid residues is generated from the precursor at a putative cleavage site for a furin-like endoprotease. In contrast to smaller LEAP-2 variants, this peptide exhibited dose-dependent antimicrobial activity against selected microbial model organisms. LEAP-2 shares some characteristic properties with classic peptide hormones and it is expected that the isolation of this novel peptide will help to unravel its physiological role. PMID:12493837

Krause, Alexander; Sillard, Rannar; Kleemeier, Burkhard; Klüver, Enno; Maronde, Erik; Conejo-García, José Ramon; Forssmann, Wolf Georg; Schulz-Knappe, Peter; Nehls, Michael C.; Wattler, Frank; Wattler, Sigrid; Adermann, Knut

2003-01-01

135

Evaluation of selected biochemical parameters in the saliva of young males using mobile phones.  

PubMed

Abstract The biochemical status in the saliva of 12 males before/after using mobile phone has been evaluated. Radio frequency signals of 1800 MHz (continuous wave transmission, 217?Hz modulate and Global System for Mobile Communications [GSM - non-DTX]) with 1.09 w/kg specific absorption rate (SAR) value were used for 15 and 30?min. Cell phone radiation induced a significant increase of superoxide dismutase (SOD); there was a statistically significant effect of talking time on the levels of SOD, F(2, 33)?=?8.084, p?

Abu Khadra, Khalid M; Khalil, Ahmad M; Abu Samak, Mahmoud; Aljaberi, Ahmad

2015-03-01

136

Effects of depletion sampling by standard three-pass pulsed DC electrofishing on blood chemistry parameters of fishes from Appalachian streams  

USGS Publications Warehouse

Adverse effects on fishes captured by electrofishing techniques have long been recognized, although the extent of associated physical injury and behavioral alterations are highly variable and dependent on a number of factors. We examined the effects of three-pass pulsed DC (PDC) electrofishing on two salmonid species (Rainbow Trout Oncorhynchus mykiss and Brook Trout Salvelinus fontinalis) and five other genera (Green Sunfish Lepomis cyanellus, Potomac Sculpin Cottus girardi, Fathead Minnow Pimephales promelas, Largemouth Bass Micropterus salmoides, and Channel Catfish Ictalurus punctatus) common to Appalachian streams. We examined the corresponding effects of PDC electroshock on the following physiological indicators of stress and trauma: blood glucose and serum lactate, as well as on other blood chemistry, namely, enzymes, electrolytes, minerals, and proteins. All species demonstrated physiological responses to PDC electroshock, indicated by the biochemical differences in blood parameters in unshocked and shocked groups of fish with or without gross evidence of hemorrhagic trauma. Serum lactate was the most consistent indicator of these effects. Significant differences in whole blood glucose levels were also noted in treatment groups in all species except Green Sunfish, although the patterns observed were not as consistent as for serum lactate. Elevations in the serum enzymes, aspartate aminotransferase and creatine kinase, in the electroshocked fish occurred only in the two salmonid species. In many instances, although blood parameters were elevated in electroshocked fish compared with the unshocked controls for a given species, there were no differences in those levels in electroshocked fish based on the presence of gross hemorrhagic trauma to axial musculature. While some of the blood parameters examined correlated with both the occurrence of electroshock and the resultant tissue injury, there was no apparent link between the altered blood chemistry and increased mortality 30 d after electrofishing.

Densmore, Christine L.; Panek, Frank M.

2013-01-01

137

Effect of Aqueous Stem Bark Extract of Khaya senegalensis on Some Biochemical, Haematological, and Histopathological Parameters of Rats  

PubMed Central

The subchronic effect of aqueous stem bark extract of Khaya senegalensis on some biochemical, haematological, and histopathological parameters of rats was investigated. The rats were divided into six groups of five rats per group. Groups I to VI were administered graded doses of 0, 400, 800, 1200, 1600, and 2000?mg/kg?bw, respectively. The result of study revealed that administration of the Khaya senegalensis for twenty-eight days at the experimental dose resulted in significant (P < 0.05) increase in urea, electrolytes (Na+, K+), and creatinine levels. The extract also significantly (P < 0.05) increased serum activity of ALT, AST, and ALP. The levels of protein, albumin, and bilirubin were significantly changed when compared to their control values, but they were not dose dependent. The hematological indices assayed in this study were not significantly affected at the experimental dose when compared to the control values. Histological studies of the liver showed cellular degeneration and necrosis and bile duct hyperplasia and fibrosis with lymphocytic infiltration of the hepatocyte, providing supportive evidence for discussing the biochemical findings, indicative of functional derangement. The histological architecture of the kidney and that of the heart were however preserved. The result of this study indicates that the aqueous stem bark extract of K. senegalensis may affect the cellular integrity of vital organs of the body. PMID:24348549

Onu, A.; Saidu, Y.; Ladan, M. J.; Bilbis, L. S.; Aliero, A. A.; Sahabi, S. M.

2013-01-01

138

Impact through time of different sized titanium dioxide particles on biochemical and histopathological parameters.  

PubMed

Due to corrosion, a titanium implant surface can be a potential source for the release of micro (MPs) and nano-sized particles (NPs) into the biological environment. This work sought to evaluate the biokinetics of different sized titanium dioxide particles (TiO2 ) and their potential to cause cell damage. Wistar rats were intraperitoneally injected with 150 nm, 10 nm, or 5nm TiO2 particles. The presence of TiO2 particles was evaluated in histologic sections of the liver, lung, and kidney and in blood cells at 3 and 12 months. Ultrastructural analysis of liver and lung tissue was performed by TEM, deposit concentration in tissues was determined spectroscopically, and oxidative metabolism was assessed by determining oxidative membrane damage, generation of superoxide anion (O2(-)), and enzymatic and non-enzymatic antioxidants. TiO2 particles were observed inside mononuclear blood cells and in organ parenchyma at 3 and 12 months. TiO2 deposits were consistently larger in liver than in lung tissue. Alveolar macrophage O2(-) generation and average particle size correlated negatively (p < 0.05). NPs were more reactive and biopersistent in lung tissue than MPs. Antioxidant activity, particularly in the case of 5 nm particles, failed to compensate for membrane damage in liver cells; the damage was consistent with histological evidence of necrosis. PMID:23775874

Bruno, Marcos E; Tasat, Deborah R; Ramos, Emilio; Paparella, María L; Evelson, Pablo; Rebagliati, Raúl Jiménez; Cabrini, Rómulo L; Guglielmotti, María B; Olmedo, Daniel G

2014-05-01

139

The effects of intellan and cytacon on hematological and biochemical parameter in rabbits: a comparative study.  

PubMed

Several plants have been selected based on their use in traditional systems of medicine, and research has identified a number of natural compounds that could act as Nootropicagents. In this study a herbal product Intellan containing Centella asiatica, Bacopa monniera, Coriandum sativum, Amomum subulatum, Emblica officinalis and another product Cytacon (Cyanocobalamine) were selected The study was designed on animal models to explore the effects on different parameters. For this the animals were given chronic dosing for 6-8 weeks during and after which the parameters were observed to determine their effects. The purpose of focusing on such formulations is to do hematological screening in long-term use. The hematological parameter included hemoglobin/HCT, total leucocyte count, platelets. The lymphocytes and the monocytes counts were increased significantly by intellan, while cyanocobalamine increases RBC counts, platelet counts, monocyte counts, hematocrit etc significantly. The SGPT, SGOT were found increased in both of these drugs. PMID:25176357

Anser, Humera; Najam, Raheela; Khan, Saira Saeed; Riaz, Bushra; Sarfaraz, Sana

2014-09-01

140

Bayesian parameter inference for stochastic biochemical network models using particle Markov chain Monte Carlo  

PubMed Central

Computational systems biology is concerned with the development of detailed mechanistic models of biological processes. Such models are often stochastic and analytically intractable, containing uncertain parameters that must be estimated from time course data. In this article, we consider the task of inferring the parameters of a stochastic kinetic model defined as a Markov (jump) process. Inference for the parameters of complex nonlinear multivariate stochastic process models is a challenging problem, but we find here that algorithms based on particle Markov chain Monte Carlo turn out to be a very effective computationally intensive approach to the problem. Approximations to the inferential model based on stochastic differential equations (SDEs) are considered, as well as improvements to the inference scheme that exploit the SDE structure. We apply the methodology to a Lotka–Volterra system and a prokaryotic auto-regulatory network. PMID:23226583

Golightly, Andrew; Wilkinson, Darren J.

2011-01-01

141

Effect of atrazine, glyphosate and quinclorac on biochemical parameters, lipid peroxidation and survival in bullfrog tadpoles (Lithobates catesbeianus).  

PubMed

Increased use of pesticides worldwide has led to damage not only to natural ecosystems but also to nontarget species. This study assessed the effects of different concentrations of the herbicides atrazine, glyphosate, and quinclorac on biochemical parameters, lipid peroxidation, and survival in tadpoles of Lithobates catesbeianus (bullfrog). Two hundred eighty-eight tadpoles were acquired from a frog farm in the south of Brazil. All animals were kept in aquariums under controlled laboratory conditions for 7 days and exposed to commercial formulations of atrazine (5, 10, and 20 ?g/L), glyphosate (36, 72, and 144 ?g/L), and quinclorac (0.05, 0.10, and 0.20 ?g/L) for 7 days thereafter. The concentrations used in this study are similar to the levels of these herbicides found in natural water bodies. After exposure, gill, liver, and muscle samples were removed from each animal for quantitation of glycogen, total lipids, triglycerides, cholesterol, total proteins, and lipid peroxidation. Atrazine, glyphosate, and quinclorac exposure induced a significant decrease in levels of glycogen and total lipids in gill, liver, and muscle. Triglycerides levels in the gill increased after exposure to glyphosate, and decreased after exposure to atrazine and quinclorac; their levels in liver and muscle decreased on exposure to all herbicides. Cholesterol and total protein levels decreased in liver and muscle for all three herbicides. All tissues exhibited increased lipid peroxidation after exposure to all herbicides. In conclusion, exposure to the herbicides tested in this study induced significant changes in biochemical parameters and increased lipid peroxidation levels in tadpoles of L. catesbeianus. PMID:24276472

Dornelles, Michele Flores; Oliveira, Guendalina Turcato

2014-04-01

142

Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins  

PubMed Central

The lack of kinetic data concerning the biological effects of reactive oxygen species is slowing down the development of the field of redox signaling. Herein, we deduced and applied equations to estimate kinetic parameters from typical redox signaling experiments. H2O2-sensing mediated by the oxidation of a protein target and the switch-off of this sensor, by being converted back to its reduced form, are the two processes for which kinetic parameters are determined. The experimental data required to apply the equations deduced is the fraction of the H2O2 sensor protein in the reduced or in the oxidized state measured in intact cells or living tissues after exposure to either endogenous or added H2O2. Either non-linear fittings that do not need transformation of the experimental data or linearized plots in which deviations from the equations are easily observed can be used. The equations were shown to be valid by fitting to them virtual time courses simulated with a kinetic model. The good agreement between the kinetic parameters estimated in these fittings and those used to simulate the virtual time courses supported the accuracy of the kinetic equations deduced. Finally, equations were successfully tested with real data taken from published experiments that describe redox signaling mediated by the oxidation of two protein tyrosine phosphatases, PTP1B and SHP-2, which are two of the few H2O2-sensing proteins with known kinetic parameters. Whereas for PTP1B estimated kinetic parameters fitted in general the present knowledge, for SHP-2 results obtained suggest that reactivity toward H2O2 as well as the rate of SHP-2 regeneration back to its reduced form are higher than previously thought. In conclusion, valuable quantitative kinetic data can be estimated from typical redox signaling experiments, thus improving our understanding about the complex processes that underlie the interplay between oxidative stress and redox signaling responses. PMID:25325054

Brito, Paula M.; Antunes, Fernando

2014-01-01

143

Blood pressure parameters as determinants of small artery structure in human essential hypertension.  

PubMed

1. Adaptive changes in small arteries may be more closely correlated with pulse pressure than with systolic, diastolic or mean blood pressures in human essential hypertension. 2. An analysis was performed on the structure of small arteries, age and blood pressure measurements obtained from 56 patients with untreated essential hypertension and 56 matched normotensive volunteers to examine the association between age, blood pressure and small artery structural parameters. 3. Essential hypertension was associated with an increase in media thickness and a decrease in lumen diameter, resulting in an increase in media/lumen ratio. 4. There was a significant correlation between age and media/lumen ratio in normotensive volunteers but not in patients with essential hypertension. 5. There was no correlation between any blood pressure and structural parameter in normotensive volunteers. 6. Both diastolic and mean blood pressures in essential hypertension correlated with media/lumen ratio (P < 0.01); systolic blood pressure correlated less well (P < 0.02). However, pulse pressure did not correlate with media/lumen ratio, suggesting that it is not a significant determinant of small artery structure in untreated essential hypertension. PMID:9205414

Cooper, A; Heagerty, A M

1997-06-01

144

Evaluation of nicotine, cotinine, thiocyanate, carboxyhemoglobin, and expired carbon monoxide as biochemical tobacco smoke uptake parameters  

Microsoft Academic Search

In a cross-sectional study on 236 individuals in Japan (174 males, 62 females; 149 smokers, 87 non-smokers) plasma nicotine (pnic), cotinine (pcot) and thiocyanate (pSCN), urinary creatinine ratios of nicotine (unic), cotinine (ucot) and thiocyanate (uSCN) as well as carboxyhemoglobin (COHb) and expired carbon monoxide (COex) were determined. All tobacco smoke uptake parameters (TSUP) were significantly elevated in smokers as

Hideo Muranakalt; Eigo Higashi; Shunro Itani; Yoshiharu Shimizu

1988-01-01

145

Protective effect of royal jelly on fertility and biochemical parameters in bleomycin-?induced male rats  

PubMed Central

Background: Bleomycin (BL) is a glycopeptide antibiotic obtained from the bacterium Streptomyces verticillus which is routinely used for treatment of human cancers. Royal jelly (RJ) is a production from the hypo pharyngeal, mandibular and post cerebral glands of nurse bees. RJ consists of 66% water, 15% sugars, 5% lipids, and 13% proteins, essential amino acids and vitamins. Objective: The aim of present study was to evaluate protective effect of royal jelly on sperm parameters and malondialdehyde (MDA) production in rat. Materials and Methods: Forty adult male wistar rats (220±20gr) were randomly divided into 4 groups (n=10). Control group (CG) received normal saline 10 ml/kg twice a week with Intraperitoneal (I.P) for 48 days (0.3 ml/rat(. Royal Jelly group (RJG) received jelly (100 mg/kg daily) for 48 days orally. Bleomycin group (BLG) received BL (10 mg/kg twice a week) with I.P for 48 days. Royal Jelly+ Bleomycin group (RJ+BLG) received royal Jelly (100 mg/kg /day) orally concomitant with BL administration. Sperm count, motility, and viability were investigated and chromatin quality and DNA integrity were also analyzed. Serum testosterone and MDA concentrations were measured as well. Results: BL caused decline significantly (p<0.05) sperm count, sperm viability, motility as well as testosterone concentration compared to control group while significant (p<0.05) increases in immature sperm, sperm with damaged DNA and MDA concentration were announced in BL in comparison with CG and RJ+BLG. Royal jelly improved Bleomycin-induced toxicity on sperm parameters and testosterone and MDA concentrations. Conclusion: The present results support the idea that BL adversely affects sperm parameters and MDA and the RJ with antioxidant properties has positive effects on these parameters. This article extracted from M.Sc. thesis. (Tayebeh amirshahi) PMID:24799882

Amirshahi, Tayebeh; Najafi, Gholamreza; Nejati, Vahid

2014-01-01

146

Relationship between depression, clinical and biochemical parameters in patients undergoing haemodialysis.  

PubMed

In this paper, we investigated the incidence of depression and its relation to clinical, laboratory parameters and sleep disorders in 45 haemodialysis (HD) patients. They were divided into two groups. Group A (n = 29) had no depression, whereas Group B (n = 16) had clinically assessed depression. Subjects were compared in terms of socioeconomic, clinical, laboratory parameters and presence of sleep disorders. Groups were matched for age, sex, family status, education, self-esteem, coffee and alcohol consumption, psychiatric history, time on HD and laboratory (serum urea, creatinine, electrolytes, iron, albumin and lipids) parameters. Group B demonstrated significantly lower haemoglobin levels (11.13 ± 1.69 and 12.23 ± 1.31 g/dl, respectively; p < 0.01) and higher C-Reactive Protein (CRP) levels (1.82 ± 1.73 and 0.83 ± 0.6 mg/dl, respectively; p < 0.005) compared to Group A. Additionally, strong correlation was observed when Hamilton Depression Scale scores were related to haemoglobin (r =-0.30, p < 0.05), CRP (r = 0.38, p < 0.001) and AIS scores (r = 0.54, p < 0.0001). In conclusion, depression seems to be related to high CRP, low haemoglobin levels and sleep disorders. PMID:22429274

Bornivelli, Christina; Aperis, Georgios; Giannikouris, Ioannis; Paliouras, Christos; Alivanis, Polichronis

2012-06-01

147

Propionate induced effects on feed intake and blood parameters in sheep  

E-print Network

), and the jugular vein (JV). The animals were fed grass pellet diets (- 22.7 % CP). Feed was offered for 90-minPropionate induced effects on feed intake and blood parameters in sheep HGD Leuvenink EJB Bleumer, P Kruys, LJGM Bongers Department of Human and Animal Physiology, Wageningen Institute of Animal

Paris-Sud XI, Université de

148

Effect of dietary sources of roasted oilseeds on blood parameters and milk fatty acid composition  

Microsoft Academic Search

The aim of this experiment was to investigate the effect of supplementing the basal diet with oilseeds on blood parameters and composition of milk fatty acids, especially conjugated linoleic acid (CLA). Forty-eight lactating Holstein cows in early lactation were used in a randomized block design. The cows in each group were fed the control basal diet (the control diet) or

Z. L. Liu; D. P. Yang; P. Chen; S. B. Lin; X. Y. Jiang; W. S. Zhao; J. M. Li; W. X. Dong

2008-01-01

149

Survey of Selected Pathogens and Blood Parameters of Northern Yellowstone Elk: Wolf Sanitation Effect Implications  

Microsoft Academic Search

The restoration or conservation of predators could reduce seroprevalences of certain diseases in prey if predation selectively removes animals exhibiting clinical signs. We assessed disease seroprevalences and blood parameters of 115 adult female elk (Cervus elaphus) wintering on the northern range of Yellowstone National Park (YNP) during 2000- 2005 and compared them to data collected prior to wolf (Canis lupus)

SHANNON M. BARBER-MEYER; P. J. WHITE; L. DAVID MECH

2007-01-01

150

Kinetic Parameters for the Noncatalyzed and Enzyme-Catalyzed Mutarotation of Glucose Using a Blood Glucometer  

ERIC Educational Resources Information Center

The kinetic parameters for the conversion of alpha-D-glucose to beta-D-glucose were measured using a blood glucometer. The reaction order, rate constant, and Arrhenius activation energy are reported for the noncatalyzed reaction and turnover number and Michaelis constant are reported for the reaction catalyzed by porcine kidney mutarotase. The…

Hardee, John R.; Delgado, Bryan; Jones, Wray

2011-01-01

151

Genetic and phenotypic relationships between blood gas parameters and ascites-related traits in broilers  

Microsoft Academic Search

Ascites, also called pulmonary hypertension syndrome, is a metabolic disorder in chickens that have an insufficient pulmonary vascular capacity. The tendency of broilers to develop ascites is heritable, and successful selection against this susceptibility would benefit from good and easy-to-measure indicator traits. Blood gas parameters have been suggested as indicator traits for ascites susceptibility. Therefore, the aim of the present

A. M. Closter; P. van As; M. A. M. Groenen; A. L. J. Vereijken; Arendonk van J. A. M; H. Bovenhuis

2009-01-01

152

Effect of bypass fat supplementation on productive performance and blood biochemical profile in lactating Murrah (Bubalus bubalis) buffaloes.  

PubMed

The study investigated the effect of dietary supplementation of bypass fat on productive performance and blood biochemical profile of lactating Murrah buffaloes (Bubalus bubalis). Fifteen multiparous buffaloes (2-4 lactation) of early to mid lactation were divided in three homogenous groups T(1) (control), T(2), and T(3) of five each. The animals in T(1) were fed with a basal diet consisting of a concentrate mixture, green sorghum, and wheat straw as per requirements, while the animals in group T(2) and T(3) were fed with same ration supplemented with 0.7 % (100 g/day) and 1.4 % (200 g/day) bypass fat (on dry matter intake (DMI) basis), respectively. The feed intake, milk yield, and milk composition were not influenced by supplemental bypass fat. However, fat-corrected milk (6.5 %) yield was higher (P < 0.05) in T(3) (14.21) than that of T(1) (9.83) and similar with T(2) (11.05). Feed efficiency (milk yield/kg DMI) was higher (P < 0.05) in group T(3) (0.51) than that of T(1) (0.38) and T(2) (0.41) indicating that buffaloes fed with bypass fat which is 1.4 % (200 g/day) of the diet were economically more efficient. The serum cholesterol level was higher (P < 0.01) in bypass fat-supplemented group (T(2) and T(3)) of animals. Serum high density lipoprotein (HDL) cholesterol (good cholesterol) level was more (P < 0.05) than LDL cholesterol (bad cholesterol) level with higher dose of bypass fat in T(3) than T(2). It was concluded that bypass fat supplementation with 1.4 % of the diet (200 g/day) increased the fat-corrected milk production and feed efficiency along with serum HDL cholesterol level in lactating Murrah buffaloes. PMID:22373930

Ranjan, Amit; Sahoo, Biswanath; Singh, Vijay Kumar; Srivastava, Susant; Singh, Suresh Pratap; Pattanaik, Ashok Kumar

2012-10-01

153

The use of blood gas parameters to predict ascites susceptibility in juvenile broilers.  

PubMed

Ascites syndrome is a metabolic disorder found in modern broilers that have insufficient pulmonary vascular capacity. Commercial breeding programs have heavily focused on high growth rate, which led to fast-growing chickens, but as a negative consequence, the incidence of ascites syndrome increased. However, not all birds with a high growth rate will suffer from ascites syndrome, which might indicate a genetic susceptibility to ascites. Information on blood gas parameters measured early in life and their relation to ascites susceptibility is expected to contribute to identification on the cause of ascites syndrome. In this study, several physiological parameters, such as blood gas parameters [pH, partial pressure of CO(2) in venous blood (pvCO(2)), and partial pressure of O(2) in venous blood], hematocrit, electrolytes (Na(+), Ca(2+), and K(+)), metabolites (lactate and glucose), were measured at d 11 to 12 of age from 100 female and 100 male broilers. From d 14 onward, the birds were challenged to provoke the development of ascites syndrome. Our results showed that high pvCO(2) values together with low pH values (males) or high pH values (females) in the venous blood of juvenile broilers coincided with ascites. Therefore, blood pvCO(2) and pH in both juvenile male and female broilers seem to be critical factors in ascites pathophysiology and can be used as phenotypic traits to predict ascites susceptibility in juvenile broilers at d 11 to 12. A prediction model was built on a subpopulation of the broilers without any loss in sensitivity (0.52) and specificity (0.78) when applied to the validation population. The parameter sex was included in the prediction model because levels of pvCO(2) and pH that associated with ascites susceptibility are different between males and females. Commercial breeders can include these phenotypic traits in their genetic selection programs to reduce the incidence of ascites syndrome. PMID:20634524

van As, P; Elferink, M G; Closter, A M; Vereijken, A; Bovenhuis, H; Crooijmans, R P M A; Decuypere, E; Groenen, M A M

2010-08-01

154

Comparison of pigeon guillemot, Cepphus columba, blood parameters from oiled and unoiled areas of Alaska eight years after the Exxon Valdez oil spill  

USGS Publications Warehouse

In 1997, we compared the haematological and plasma biochemical profiles among populations of pigeon guillemots, Cepphus columba, in areas oiled and not oiled by the 1989 Exxon Valdez oil spill (EVOS) that occurred in Prince William Sound (PWS), Alaska. Pigeon guillemot populations in PWS were injured by EVOS and have not returned to pre-spill levels. If oil contamination is limiting recovery of pigeon guillemots in PWS, then we expected that blood parameters of pigeon guillemots would differ between oiled and unoiled areas and that these differences would be consistent with either toxic responses or lower fitness. We collected blood samples from chicks at approximately 20 and 30 days after hatching. Physiological changes associated with chick growth were noted in several blood parameters. We found that only calcium and mean cell volume were significantly different between the chicks in oiled and unoiled areas. Despite these differences, blood biomarkers provided little evidence of continuing oil injury to pigeon guillemot chicks, eight years after the EVOS. Preliminary data from adults indicated elevated aspartate aminotransferase activity in the adults from the oiled area, which is consistent with hepatocellular injury. Because adults have greater opportunities for exposure to residual oil than nestlings, we recommend studies that fully evaluate the health of adults residing in oiled areas. (C) 2000 Elsevier Science Ltd.

Seiser, P.E.; Duffy, L.K.; McGuire, David A.; Roby, D.D.; Golet, G.H.; Litzow, M.A.

2000-01-01

155

Blood  

MedlinePLUS

... mysterious, life-sustaining fluid called blood. What Is Blood and What Does It Do? Two types of ... mixture of blood cells and plasma. Continue Red Blood Cells Red blood cells (RBCs, and also called ...

156

Blood  

MedlinePLUS

... solid part of your blood contains red blood cells, white blood cells, and platelets. Red blood cells deliver oxygen from your lungs to your tissues and organs. White blood cells fight infection and are part of your body's ...

157

The Effect of Ramadan Fasting on Biochemical and Performance Parameters in Collegiate Wrestlers  

PubMed Central

Objective(s) This study was designed to evaluate the effect of Ramadan fasting on body composition, aerobic and anaerobic power, strength, plasma lipids profile and serum glucose among collegiate wrestlers. Materials and Methods Fourteen male collegiate wrestlers (age, 20.12±2.5 yrs) volunteered as subjects for the study. Subjects were tested one week before the beginning of Ramadan, the last two days of Ramadan and the last two days of the 4th week after the end of Ramadan. The paired sample t-test was used to assess the differences in pre and post-performance tests and repeated measures ANOVA and Bonferroni post hoc test to determine differences between three blood samplings. Results The results showed that except for anaerobic power and strength, body weight, body fat percentage and aerobic power at 4th week of Ramadan were significantly lower than pre-Ramadan values (P=0.05). Total cholesterol and low density lipoprotein levels decreased after Ramadan compared to pre-Ramadan (P= 0.011, P=0.001), however, a month after Ramadan, it reached to higher levels compared to pre-Ramadan period, which were not statistically significant. Similarly, significant decrease and increase were consequently observed in glucose and high density lipoprotein (P=0.001, P=0.045). Triacylglycerol and VLDL increased at the end of Ramadan compared to the period of time before Ramadan, and after Ramadan, it reached the lower levels compared to concentrations before Ramadan (P=0.133, P=0.133). Conclusion This study also indicated that Ramadan fasting appears to have significant effect on body composition, aerobic power and lipid profile. PMID:23653853

Mirzaei, Bahman; Rahmani-Nia, Farhad; Moghadam, Mahdi Ghahremani; Ziyaolhagh, Seyed Javad; Rezaei, Abolfazl

2012-01-01

158

Prognostic Judgment at Post-Surgery by Biochemical Parameters in Beef Cattle with Left Displaced Abomasum  

PubMed Central

ABSTRACT We examined whether the postoperative prognosis of beef cattle with left displaced abomasum (LDA) can be estimated from changes in laboratory parameters. Preoperatively, beef cattle with LDA showed increases in plasma glucose with decreased serum insulin in the glucose tolerance test compared to non-LDA cattle. Postoperatively, the cattle with LDA were retrospectively divided into two groups, good and bad prognoses. Although plasma glucose concentrations significantly increased either pre- or postoperatively, no difference was noted between the good and bad prognosis groups. Serum insulin concentrations in the bad prognosis group significantly decreased, compared to those in the good prognosis group. These findings suggest that beef cattle with LDA elicit disturbed glucose metabolite pre- and postoperatively, and serum insulin levels may predict their prognoses after surgery. PMID:24998331

ICHIJO, Toshihiro; SATOH, Hiroshi; YOSHIDA, Yuki; MURAYAMA, Isao; TAGUCHI, Kiyoshi; SATO, Shigeru

2014-01-01

159

Prognostic judgment at post-surgery by biochemical parameters in beef cattle with left displaced abomasum.  

PubMed

We examined whether the postoperative prognosis of beef cattle with left displaced abomasum (LDA) can be estimated from changes in laboratory parameters. Preoperatively, beef cattle with LDA showed increases in plasma glucose with decreased serum insulin in the glucose tolerance test compared to non-LDA cattle. Postoperatively, the cattle with LDA were retrospectively divided into two groups, good and bad prognoses. Although plasma glucose concentrations significantly increased either pre- or postoperatively, no difference was noted between the good and bad prognosis groups. Serum insulin concentrations in the bad prognosis group significantly decreased, compared to those in the good prognosis group. These findings suggest that beef cattle with LDA elicit disturbed glucose metabolite pre- and postoperatively, and serum insulin levels may predict their prognoses after surgery. PMID:24998331

Ichijo, Toshihiro; Satoh, Hiroshi; Yoshida, Yuki; Murayama, Isao; Taguchi, Kiyoshi; Sato, Shigeru

2014-10-01

160

Attenuation of Biochemical Parameters in Streptozotocin-induced Diabetic Rats by Oral Administration of Extracts and Fractions of Cephalotaxus sinensis  

PubMed Central

Cephalotaxus sinensis (C. sinensis) large size, evergreen tree common in China and utilized for numerous effective pharmacological applications in Chinese traditional medicine. The hepato-renal effects of C. sinensis were evaluated in vivo using Streptozotocin (STZ)-induced diabetic rats as an tentative model. Animals were orally treated with 80% EtOH extract (aq.EE), H2O extract (WtE) and ethylacetate (EaF)/butanol fractions (BtF) of C. sinensis (200 mg/kg, b.w.) for 28 days whereas control received vehicle merely. The degree of fortification was measured by using biochemical parameters like serum transaminases (ALT and AST), alkaline phosphatase (ALP), creatinine, urea and urine sugar. Meanwhile, the histopathological studies were conducted out to support the above parameters. Administration of C. sinensis aq.EE/BtF (p<0.05) and EaF (p<0.01) patently prevented STZ-induced elevation levels of serum ALT, AST, ALP, creatinine, urea, urine sugar and increase body weight respectively, which were comparable with the standard drug tolbutamide, while WtE did not show any significant effect (p>0.05). Phytochemical studies revealed the presence of saponins, terpenes, sterols and flavonoids in C. sinensis which could be responsible for the possible hepato-renal protective action. The results sustain the fact that the extract/fractions of C. sinensis have an immense potential to be developed further into a phytomedicine. PMID:18231626

Saeed, Muhammad K.; Deng, Yulin; Dai, Rongji

2008-01-01

161

Assessment of some blood parameters as potential markers of hepatic copper accumulation in cattle.  

PubMed

The increasing frequency of copper (Cu) toxicosis episodes in cattle in recent years, mainly associated with excess Cu supplementation, underscores the need to identify animals in the clinically silent phase of hepatic Cu accumulation. The aim of the study reported here was to evaluate the suitability of various blood parameters as potential early markers of hepatic Cu accumulation in cattle. Paired liver and blood samples from 70 calves aged 6 to 10 months were obtained at slaughter in a region in northwestern Spain where animals usually have hepatic Cu concentration higher than safe values. Neither serum Cu concentration nor ceruloplasmin (CP) concentration, the 2 parameters most commonly used for diagnosis of Cu deficiency, were significantly associated with hepatic Cu concentration. However, whole-blood Cu concentration had a slight but significant correlation with hepatic Cu concentration (r = 0.269, P = 0.026). The use of calculated blood parameters, such as the serum or whole-blood non-CP Cu fraction, or the CP-to-serum Cu ratio, increased the correlation with the hepatic Cu concentration (r = 0.393, P = 0.001, in the case of whole-blood, non-CP Cu concentration), but the strength of the association remained insufficient for accurate prediction of hepatic Cu values. Likewise, hepatic enzyme (aspartate transaminase and gamma-glutamyltransferase) activities were significantly or nearly correlated significantly with hepatic Cu concentration, but the strength of the association remained too low for accurate prediction. It is concluded that direct analysis of Cu concentration in liver biopsy specimens--preferably postmortem liver samples obtained at slaughter--is the best technique currently available for detecting chronic subclinical Cu accumulation in cattle at risk for supraoptimal Cu exposure. PMID:16566259

López-Alonso, Marta; Crespo, Antonio; Miranda, Marta; Castillo, Cristina; Hernández, Joaquín; Benedito, José Luis

2006-01-01

162

Contamination of the River Ganga and its toxic implication in the blood parameters of the major carp Labeo rohita (Ham).  

PubMed

A field study was conducted to examine different physicochemical properties of water and various haematological and biochemical parametres of the fish Labeo rohita collected from the Ganga River (National river of India) at Varanasi district, India. The water was found to be greatly contaminated with a number of dissolved metals (Fe, Cr, Zn, Cu, Mn, Ni and Pb) whose concentrations were above the safe limits suggested by Bureau of Indian Standard (BIS 1991) for drinking water (Fe, 1,353.33 %; Cr, 456 %; Mn, 553.33 %; Ni, 4,490 % and Pb, 1,410 %). The metal accumulation in the fish blood was very high (Fe, 2,408 %; Cr, 956.57 %; Zn, 464.90 %; Cu, 310.57 %; Mn, 1,115.48 %) in comparison to the control fish maintained under strict quality control. Lower values of the various haematological parameters (total erythrocytes count, haemoglobin, haematocrit, mean corpuscular volume and O2-carrying capacity) in the river fish in comparison to the control indicate toxic manifestation exerted by the contaminated river water on the fish. The higher level of total leucocytes count further illustrates stressed condition of the river fish. The toxic impact of the Ganga water is also expressed in the fish by the presence of higher levels of cholesterol, glucose, elevated activities of the enzymes aspartate amino transferase and alanine amino transferase, and lowered protein concentration. PMID:23456946

Vaseem, Huma; Banerjee, T K

2013-08-01

163

Physiological response of dromedary camels to road transportation in relation to circulating levels of cortisol, thyroid hormones and some serum biochemical parameters.  

PubMed

Transportation is often considered as one of the main causes of stress raising considerable interest, both in economic and animal welfare terms. The objective of the current study was to determine physiological response of dromedary camels to road transportation in relation to circulating levels of cortisol, thyroid hormones and some serum biochemical factors during summer conditions. Ten Iranian dromedary camels, five males and five females, were selected for the study. The study was conducted on three consecutive days in August 2008. At first day, blood samples were collected at 08:30 A.M., 09:30 A.M. and 01:30 P.M. to determine any possible variation in individual measurements due to diurnal changes or as a result of food and water deprivation for 5 h. Travel commenced on day 2 at 08:30 A.M. for 5 h, with a total of about 300 km traveled. At second day, blood samples were collected immediately before loading, at 08:30 a.m., after 1 h transport, at 09:30 A.M., and on the end of transportation, after unloading, at 01:30 P.M. Final blood sample was taken 24 h after arrival. In the current study no significant difference was observed in any parameter between sexes at each sampling time. The data related to day before transport had no significant differences between different times except for values obtained for cortisol that at 01:30 P.M. showed a significant decrease in comparison with data at 08:30 and 09:30. Circulating cortisol, T(4), T(3) and fT(4) levels was significantly higher after transportation compared with pre-transport values and returned to basal values within 24 h after transport. Transportation had effects on metabolism as demonstrated by increase in serum concentrations of glucose, NEFA, and urea nitrogen. Serum concentrations of glucose, NEFA, and urea nitrogen returned to basal values in final bleeding at 24 h after transport termination. In the current study transportation had no significant effects on serum concentrations of fT(3), triglycerides, cholesterol, beta-hydroxybutyrate, albumin and total protein. Taken together, the results obtained for short road transportation of dromedary camels showed a strong physiological response and provide some biomarkers for stress detection in this species. Further research to validate these potential biomarkers is necessary. PMID:19544085

Saeb, M; Baghshani, H; Nazifi, S; Saeb, S

2010-01-01

164

Effect of Aqueous Extract of Passiflora edulis on Biochemical and Hematological Parameters of Wistar Albino Rats  

PubMed Central

Passiflora edulis is traditionally used in folk lore medicine for the treatment of various ailments. To validate its use in traditional medicine, it is important to evaluate its toxicity in the animal system. Therefore, this study aimed to evaluate the toxicological effects of oral administration of aqueous leaf extract of P. edulis in Wistar albino rats. Acute toxicity tests were conducted by the oral administration of 200, 500, 1000 and 2000 mg/kg body weight of the animal. In subacute study, they were administered with various doses of aqueous extract of P. edulis (100, 200, 300, and 400 mg/kg body weight) to evaluate its toxicity for a period of 7 days. The effect of aqueous extract of P. edulis on organ weight, hematological, renal, and hepatic markers were analyzed. In acute toxicity study, no mortality was seen with in 24 h of the administration of P. edulis extract. No signs of neurological and behavioral changes were noticed with in 72 h. In the subacute study, the extract intake has not changed the hematological parameters such as RBC, WBC, and platelets and it was also found that the plasma level of amino transferases, ALP, urea, uric acid and, creatinine were also not altered by the administration of P. edulis extract throughout the study. The weight of organ was found to be unaltered in all the doses selected. The acute toxicity study reveals that the oral administration of the extract was found to be safe up to the dose level of 2000 mg/kg. The subacute study indicates that the extract is safe on the bone marrow function and it is neither hepatotoxic nor nephrotoxic. This supports the safety use of the aqueous extract of P. edulis in pharmacological studies. PMID:22736906

Devaki, K.; Beulah, U.; Akila, G.; Gopalakrishnan, V. K.

2012-01-01

165

Effect of soaked Moringa oleifera seeds on growth rates and the levels of some biochemical parameters in albino rats.  

PubMed

Moringa oleifera is a multipurpose tree, cultivated in the tropics and sub-tropics for its nutritional and therapeutic properties. The raw matured seeds which have been reported to be used as food and clarifying agent of turbid water caused growth retardation in albino rats and this might have been due to its antinutritional contents. Owing to these adverse factors, the effect of soaking the seeds for 30 min and then compounding it as feeds was done. Its effect on growth rate and the level of some biochemical parameters on rats were investigated. The Wistar albino rats were fed for 21 days and their weights measured at 2 days interval. Aspartate and Alanine transaminases, Alkaline phosphatase and total bilirubin levels were assayed using Automated Vitros 350 Chemistry Analyzer. The growth rates of rats fed with the commercial rat pellets, Casilan diet and the processed Moringa seed diet had a range of 80.06 +/- 3.54 to 100.98 +/- 5.37, 66.70 +/- 7.54 to 55.23 +/- 7.47 and 52.99 +/- 4.15 to 35.47 +/- 2.26, respectively. The parameters assayed for the group that received the processed Moringa seed diet are 144.00 +/- 16.80 (AST), 41.00 +/- 7.05 (ALT), 66.50 +/- 8.80 (ALP) and 12.45 +/- 1.18 (Total Bilirubin). The one-way ANOVA statistical analysis indicated that there was no significant change in the parameters of all the groups at 95% significance level (p < 0.05). Hence, the soaked Moringa oleifera seed did not support growth in the animal subjects and also did not pose a threat to the liver. However, it is better to develop more suitable processing methods to improve the seed's nutritional capabilities. PMID:24199486

Igwilo, I O; Ogoke, T J; Ogbu, D O; Igwilo, S N; Obi, E; Abdulsalami, M S

2013-01-01

166

EFFECTS OF ACUTE EXPOSURE TO CONCENTRATED AMBIENT PARTICULATES ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS HEALTHY AND MONOCROTALINE-TREATED SPRAGUE-DAWLEY RATS  

EPA Science Inventory

EFFECTS OF ACUTE EXPOSURE TO CONCENTRATED AMBIENT PARTICULATES ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN HEALTHY AND MONOCROTALINE-TREATED SPRAGUE-DAWLEY RATS. LB Wichers1, JP Nolan2, DW Winsett2, UP Kodavanti2, MCJ Schladweiler2, DL Costa2, and WP ...

167

Baseline blood trihalomethanes, semen parameters and serum total testosterone: a cross-sectional study in China.  

PubMed

Toxicological studies showed that trihalomethanes (THMs), the most abundant classes of disinfection by-products (DBPs) in drinking water, impaired male reproductive health, but epidemiological evidence is limited and inconsistent. This study aimed to examine the associations of baseline blood THMs with semen parameters and serum total testosterone in a Chinese population. We recruited 401 men seeking semen examination from the Reproductive Center of Tongji Hospital in Wuhan, China between April 2011 and May 2012. Baseline blood concentrations of THMs, including chloroform (TCM), bromodichloromethane (BDCM), dibromochloromethane (DBCM), and bromoform (TBM) were measured using SPME-GC/ECD method. Semen quality and serum total testosterone were analyzed. Multivariable linear regressions were used to assess the associations of baseline blood THM concentrations with semen parameters and serum total testosterone levels. We found that baseline blood THM concentrations were not associated with decrements in sperm motility, sperm straight-line and curvilinear velocity. However, moderate levels of BDCM (?=-0.13 million; 95% CI: -0.22, -0.03) and DBCM (?=-4.74%; 95% CI: -8.07, -1.42) were associated with decreased sperm count and declined sperm linearity compared with low levels, respectively. Suggestive dose-response relationships were also observed between elevated blood TCM or ? THMs (sum of TCM, BDCM, DBCM and TBM) concentration and decreased sperm concentration (both p for trend=0.07), and between elevated blood DBCM concentration and decreased serum total testosterone (p for trend=0.07). Our results indicate that elevated THM exposure may lead to decreased sperm concentration and serum total testosterone. However, the effects of THM exposure on male reproductive health still warrant further studies in humans. PMID:23454109

Zeng, Qiang; Li, Min; Xie, Shao-Hua; Gu, Long-Jie; Yue, Jing; Cao, Wen-Cheng; Zheng, Dan; Liu, Ai-Lin; Li, Yu-Feng; Lu, Wen-Qing

2013-04-01

168

Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation  

PubMed Central

Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and ?-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue. PMID:23008814

Mishra, Arun K.; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

2012-01-01

169

Physiological and biochemical parameters in response to electroejaculation in adult and yearling anesthetized pampas deer (Ozotoceros bezoarticus) males.  

PubMed

The aim of this study was to characterize changes in physiological and biochemical parameters during electroejaculation (EE) under general anaesthesia in adult and yearling pampas deer males (Ozotoceros bezoarticus). The relation between heart rate, pulse rate, respiratory rate and oximetry with EE voltages was studied. The changes in cortisol, alkaline phosphatase, creatine kinase, aspartate aminotransferase and rectal temperature were determined before and after electroejaculation (BEE and AEE). Heart rate and pulse rate values increased during EE, with a greater increase in heart rate in adults (p < 0.01). Respiratory rate and SpO(2) were not affected by EE or category. The rectal temperature decreased in adults and yearlings during EE (p = 0.0001). Alkaline phosphatase, creatine kinase and aspartate aminotransferase concentrations increased AEE (p < 0.001, p < 0.01 and p?

Fumagalli, F; Villagrán, M; Damián, J P; Ungerfeld, R

2012-04-01

170

Effects of lycopene supplementation in both maternal and offspring diets on growth performance, antioxidant capacity and biochemical parameters in chicks.  

PubMed

This study investigated the effects of different supplementation ways of lycopene during pre-hatch (from the diet of hens) and post-hatch (from the diet of progeny) on production performance, antioxidant capacity and biochemical parameters in chicks. In total, 360 hens were fed diets supplemented with 0 (control group) or 40 mg lycopene/kg diet. From 28 to 34 days after the start of supplementation (30 weeks old), 650 qualified eggs were collected to artificial incubation. In this trial, 2 × 2 factorial designs were used. Male chicks hatched from hens fed with 0 or 40 mg lycopene/kg diet were fed a diet containing either 0 or 40 mg lycopene/kg diet. The results showed that, relative to control, in ovo-deposited lycopene significantly increased chick birth body weight, improved liver total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) and glutathione to oxidized glutathione ratio (GSH: GSSG), and significantly declined liver malondialdehyde (MDA) level and increased liver lycopene content during 0-14 days after hatching. On days 14 after hatching, dietary lycopene in diet began to take over gradually. Both supplementation ways of lycopene increased immune organ index, serum high-density lipoprotein (HDL) cholesterol, villus length and villus/crypt in duodenum, jejunum and ileum. Data in this study suggested lycopene supplementation could improve antioxidant capacity and immune function, and regulate lipid metabolism in chicks. PMID:24773606

Sun, B; Chen, C; Wang, W; Ma, J; Xie, Q; Gao, Y; Chen, F; Zhang, X; Bi, Y

2015-02-01

171

The effects of sage extract feed supplementation on biochemical parameters, weight of internal organs and Salmonella counts in chickens.  

PubMed

We investigated the effects of dietary addition of sage extract on the biochemical parameters, weight of some body organs and changes in the counts of Salmonella Enteritidis PT4 (SE) in experimentally infected chickens. The following diets were used: basal diet, basal diet with addition of an extract of Salvia officinalis L. (S), basal diet and SE, and basal diet and S and SE (SSE). Compared to the SE group, sage extract in the SSE group decreased activities of ALP and ALT and concentrations of glucose and bilirubin on the 4th day post inoculation (p.i.). However, on the 18th day p.i., lower levels of bilirubin and ALT activity only were detected. Addition of sage extract to the diets decreased the counts of Salmonella in the liver, spleen and caecum at both sampling times, along with lower production of mucus in the chickens' intestines. Our results suggest that the addition of sage extract to the diet could be effective in protecting SE-infected chickens. PMID:22587934

Piesova, Elena; Makova, Zuzana; Levkut, Martin; Faixova, Zita; Pistl, Juraj; Marcin, Andrej; Levkut, Mikulas

2012-12-01

172

Quality evaluation of the edible blue-green alga Nostoc flagelliforme using a chlorophyll fluorescence parameter and several biochemical markers.  

PubMed

Nostoc flagelliforme is an edible blue-green alga with herbal and dietary values. Due to the diminishing supply of natural N. flagelliforme and the large investment on the development of its cultivation technology, it is anticipated that artificially cultured N. flagelliforme will soon sustain the market supply. Once this change occurs, the storage-associated quality problem will become the focus of attention for future trade. In this paper, we used a chlorophyll fluorescence parameter, maximum quantum efficiency of Photosystem II (Fv/Fm), and several biomarkers to evaluate the quality of several N. flagelliforme samples. It was found that longer storage times resulted in darker coloured solutions (released pigments) and decreased amounts of chlorophyll a (Chl a) and water-soluble sugars (WSS). Additionally, a higher Fv/Fm value suggests better physiological recovery and quality. In actual application, determination of Fv/Fm would be the first step for evaluating the quality of N. flagelliforme, and the biochemical indexes would serve as good secondary markers. PMID:24054244

Gao, Xiang; Yang, Yiwen; Ai, Yufeng; Luo, Hongyi; Qiu, Baosheng

2014-01-15

173

Changes in immunity parameters and blood cytokine concentrations after chronic nitrile acrylate intoxication.  

PubMed

Experiments on outbred albino rats showed that chronic nitrile acrylate intoxication (60 days, 0.05 LD50 per day subcutaneously) led to reduction of T-dependent humoral immune response (T-independent humoral immune response was less affected); parameters cell immunity were suppressed to a greater extent than parameters of humoral immune reactions. Equal attenuation of the functions of Th1 and Th2 lymphocytes, decrease of the blood levels of immunoregulatory, pro- and anti-inflammatory cytokines (IFN-?, IL-2, IL-4, IL-6, IL-10, IL-13), and decrease of acetyl cholinesterase activity in thymic and splenic T lymphocytes were observed. PMID:25435468

Zabrodskii, P F; Gromov, M S; Maslyakov, V V

2014-12-01

174

Photoacoustic and photothermal cytometry for monitoring multiple blood rheology parameters in vivo  

PubMed Central

Alterations of blood rheology (hemorheology) are important for the early diagnosis, prognosis, and prevention of many diseases, including myocardial infarction, stroke, sickle cell anemia, thromboembolism, trauma, inflammation, and malignancy. However, real-time in vivo monitoring of hemorheological status using multiple parameters over long periods of time has not been reported. Here we describe the capability of label-free photoacoustic (PA) and photothermal (PT) flow cytometry in detection and imaging modes for dynamic monitoring of rheological parameters in circulating blood. We show that this integrated platform can simultaneously measure the main rheological parameters and may improve their diagnostic value. Using phenomenological approaches, we analyze correlations of PT and PA signal characteristics in the dynamic modes with red blood cell (RBC) aggregation, deformability, shape (e.g., as in sickle cells), intracellular hemoglobin distribution, individual cell velocity, flux of RBCs, and likely shear rate. Proof of concept is demonstrated in ex vivo and in vivo tests, including high-speed PT imaging of RBC shape in pathological conditions and identification of sickle cells in a mouse model of human sickle cell disease. These studies revealed the potential of this new platform integrating PT, PA, and conventional optical techniques for translation to use in humans using safe, portable, laser-based medical devices for point-of-care screening of disease progression and therapy efficiency. PMID:21948731

Galanzha, Ekaterina I.; Zharov, Vladimir P.

2012-01-01

175

Impact of feeding on growth performance, blood biochemical and mineral profiles of pre-pubescent camels under pastoral management in arid western Rajasthan.  

PubMed

The experiment was undertaken on 20 pre-pubescent dromedary camels of 300?±?7.0 kg average bodyweight (BW) and 1.5-2 years of age to determine change in body weight, blood biochemical and mineral profiles and morphometric parameters on rangeland grazing (GR) and supplementary stall feeding (GS) at arid northwestern part of Rajasthan over a period of 80 days. GR camels were maintained only on grazing, while GS camels were fed semi-intensively ad-lib groundnut haulms along with formulated concentrate and mineral mixture to meet maintenance requirement. Intake of young camels was determined using chromium(III) oxide as internal marker. The biomass yield of rangeland estimated was 0.54 t DM/ha. Dry matter intake (kg/day) by GR camels on rangeland grazing was lower than that by GS camels fed on supplementary feeding. The nutrient digestibility of dry matter and organic matter (P?parameters, body length and body height showed more significant increase in GS compared to GR camels. Intake of digestible crude protein (DCP) by GR group was 43% low compared to the requirement for growing camel of 300 kg body weight. Despite this, GR camels were able to maintain body weight due to better recycling of urea which was significantly higher in comparison to GS camels. The findings of significantly (P?

Saini, Nirmala; Kiradoo, Baldev Dass; Bohra, Dau Lal

2014-08-01

176

Morin attenuates blood pressure and oxidative stress in deoxycorticosterone acetate-salt hypertensive rats: a biochemical and histopathological evaluation.  

PubMed

The present study was designed to evaluate the antihypertensive and antioxidant effect of morin, a flavonoid against deoxycorticosterone acetate (DOCA)-salt induced hypertension in male Wistar rats. Hypertension was induced in uninephrectomized rats (UNX) by weekly twice subcutaneous injection of DOCA (25mg/kg) and 1% NaCl in the drinking water for six consecutive weeks. The DOCA-salt hypertensive rats showed significant (P < .05) increase in the systolic and diastolic blood pressure, heart rate, water intake and organ weights (kidney, heart, aorta and liver). DOCA-salt hypertensive rats also showed significant (P < .05) increase in the levels of thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes in plasma and tissues (kidney, heart, aorta and liver), and significant (P < .05) decrease in the body weight, nitrite and nitrate levels in plasma and heart. Furthermore, the activities of enzymic antioxidants such as superoxide dismutase, catalase and glutathione peroxidase in erythrocyte and tissues and the levels of non-enzymic antioxidants such as reduced glutathione, vitamin C and vitamin E in plasma and tissues were significantly (P < .05) decreased in DOCA-salt rats. Morin supplementation (50mg/kg) daily for six weeks brought back all the above parameters to near normal level. The above findings were confirmed by the histopathological examination. No significant (P < .05) effect was observed in UNX-rats treated with morin (50mg/kg). These results suggest that morin acts as an antihypertensive and antioxidant agent against DOCA-salt induced hypertension. PMID:22386933

Prahalathan, Pichavaram; Kumar, Subramanian; Raja, Boobalan

2012-08-01

177

Key parameters in blood-surface interactions of 3D bioinspired ceramic materials.  

PubMed

Direct contact of materials with blood components may trigger numerous processes which ultimately lead to hemolysis, clot formation and recruitment of inflammatory cells. In this study, the blood-surface interactions for two inert bioinspired ceramic scaffolds obtained from natural resources; biomorphic carbon and silicon carbides (bioSiC) from different origins have been studied. The response of the blood in contact with carbon is well known, however little has been identified on the influence of their 3D porous structure. Moreover, to our knowledge, there is no reference in the literature about the hemocompatibility of biomorphic silicon carbide as a porous scaffold. The experimental results showed the surface energy to be crucial to evaluate the hemocompatibility of a material however the surface topography and material porosity are also parameters to be considered. Surface roughness modifies clot formation whereas for protein adsorption total sample porosity seems to be the key parameter to be considered for hydrophilic materials (biomorphic silicon carbides), while the size of the pores determines the hemolytic response. PMID:24907756

Díaz-Rodríguez, P; González, P; Serra, J; Landin, M

2014-08-01

178

Effects of Salinity on Leaf Spectral Reflectance and Biochemical Parameters of Nitrogen Fixing Soybean Plants (Glycine max L.)  

NASA Astrophysics Data System (ADS)

Measurements of physiology and hyperspectral leaf reflectance were used to detect salinity stress in nitrogen fixing soybean plants. Seedlings were inoculated with suspension of Bradyrhizobium japonicum strain 273. Salinity was performed at the stage of 2nd-4th trifoliate expanded leaves by adding of NaCl in the nutrient solution of Helrigel in concentrations 40 mM and 80 mM. A comparative analysis was performed between the changes in the biochemical parameters - stress markers (phenols, proline, malondialdehyde, thiol groups), chlorophyll a and b, hydrogen peroxide, and leaf spectral reflectance in the spectral range 450-850 nm. The spectral measurements were carried out by an USB2000 spectrometer. The reflectance data of the control and treated plants in the red, green, red-edge and the near infrared ranges of the spectrum were subjected to statistical analysis. Statistically significant differences were found through the Student's t-criterion at the two NaCl concentrations in all of the ranges examined with the exception of the near infrared range at 40 mM NaCl concentration. Similar results were obtained through linear discriminant analysis. The tents of the phenols, malondialdehyde and chlorophyll a and b were found to decrease at both salinity treatments. In the spectral data this effect is manifested by decrease of the reflectance values in the green and red ranges. The contents of proline, hydrogen peroxide and thiol groups rose with the NaCl concentration increase. At 80 mM NaCl concentration the values of these markers showed a considerable increase giving evidence that the soybean plants were stressed in comparison with the control. This finding is in agreement with the results from the spectral reflectance analysis.

Krezhova, Dora D.; Kirova, Elisaveta B.; Yanev, Tony K.; Iliev, Ilko Ts.

2010-01-01

179

Laser reflectance oximetry and Doppler flowmetry in assessment of complex physiological parameters of cutaneous blood microcirculation  

NASA Astrophysics Data System (ADS)

The integration of multiple optical techniques within a single diagnostic device is used to address the difficulties in standardising measurement of cutaneous blood micro-dynamics caused by high variability. We demonstrate the benefits of simultaneous assessment of blood relative volume (Vb), microcirculation index (Im) and tissue oxygen saturation (StO2), during long-term examination of healthy volunteers. Consequently, five rhythmic components: endothelial, neurogenic, myogenic, breath and heart pulses were established showing high variability up to 30 - 50% as well as in initial parameters around 16%. All rhythmic components were synchronous with some latency between Im and StO2 in the myogenic component supports the hypothesis of strong correlation between peripheral hemodynamics and oxygen utilisation in tissues.

Dunaev, Andrey V.; Sidorov, Victor V.; Stewart, Neil A.; Sokolovski, Sergei G.; Rafailov, Edik U.

2013-03-01

180

Biochemical and steroid concentrations in follicular fluid and blood plasma in different follicular waves of the estrous cycle from normal and superovulated beef cows.  

PubMed

The objectives of the current study were to (i) define the changes in size and number of follicles populations, (ii) determine the follicular fluid (FF) biochemical and steroid concentrations collected from different-sized follicles (5-9 and ? 10 mm) and (iii) compare between biochemical and hormonal concentrations of FF with those in blood plasma in relation to the first two follicular waves of the estrous cycle (days 4 and 13) from normal and cows primed for superovulation. After estrus, cows (n=20) were assigned randomly to each of four treatment groups. Group 1: ovariectomy on day 4 (day 0 = ovulation). Group 2: FSH treatment and ovariectomy on day 4. Group 3: dominant follicle ablation (DFA) on day 8 and ovariectomy on day 13. Group 4: DFA on day 8, FSH treatment and ovariectomy on day 13. Blood samples were collected and FF was aspirated and pooled per follicle class within cow to determine glucose, urea, triglycerides, cholesterol, total protein, albumin, lactate dehydrogenase, alkaline phosphatase, gamma-glutamyl transpeptidase, aspartate aminotransferase, creatin phosphokinase, estradiol-17? and progesterone concentrations. Follicular class×follicular wave interaction was detected for albumin and lactate dehydrogenase. Results showed that FF concentrations of cholesterol increased from medium to large follicles and decreased for urea and aspartate aminotransferase. Tryglycerides and total protein were greater in the second than in the first follicular wave. FSH treatment decreased FF alkaline phosphatase, E2 and P4 concentrations. Quantitative differences between these fluids are discussed with respect to follicular development. PMID:24139762

Aller, J F; Callejas, S S; Alberio, R H

2013-11-30

181

Biochemical markers of fatal hypothermia.  

PubMed

The aim of this study was to investigate the usefulness of postmortem biochemical investigations in the diagnosis of fatal hypothermia. 10 cases of fatal hypothermia and 30 control cases were selected. A series of biochemical parameters, such as glucose, acetone, 3-beta-hydroxybutyrate, isopropyl alcohol, free fatty acids, adrenaline, growth hormone, adrenocorticotropic hormone, thyroid-stimulating hormone, cortisol, calcium, magnesium, C-reactive protein, procalcitonin as well as markers of renal and cardiac functions were measured in blood, postmortem serum from femoral blood, urine, vitreous and pericardial fluid. The results suggested that deaths due to hypothermia, especially in free-ethanol cases, are characterized by increased ketone levels in blood and other biological fluids, increased adrenaline concentrations in urine, increased cortisol levels in postmortem serum from femoral blood and increased free cortisol values in urine. Increased or decreased levels of other biological parameters are either the result of terminal metabolic changes or the expression of preexisting diseases and may provide information to elucidate the death process on a case-by-case basis. PMID:23313602

Palmiere, Cristian; Bardy, Daniel; Letovanec, Igor; Mangin, Patrice; Augsburger, Marc; Ventura, Francesco; Iglesias, Katia; Werner, Dominique

2013-03-10

182

The prevention of ischemia/reperfusion induced oxidative damage by venous blood in rabbit kidneys monitored with biochemical, histopatological and immunohistochemical analysis.  

PubMed

The purpose of this study was to investigate the biochemical, histopathological and immunohistochemical effects of venous blood on ischemia/reperfusion-induced oxidative DNA damage and mutation in rabbit kidneys in comparison to melatonin treatment, which has a known protective effect against ischemia/reperfusion (IR) injury. The rabbits were divided into five groups: renal ischemia (RI), renal ischemia-reperfusion (RIR), renal ischemia-venous blood-reperfusion (RIVR), melatonin + renal ischemia-reperfusion (MRIR), and the healthy sham control group (HG). Melatonin (2.5 mg/kg delivered intraperitoneally) was administered one hour prior to ischemia. In the RIVR group, 1 ml of venous blood was administered 5 minutes before the reperfusion. The xanthine oxidase activity in the kidney tissue was determined as 53.50 ± 1.72, 31.00 ± 6.39, 45.66 ± 9.20, 28.66 ± 6.05 and 14.33 ± 1.28 U/g protein; the MDA levels were 6.32 ± 0.02, 19.50 ± 1.33, 7.00 ± 0.96, 7.50 ± 0.76 and 4.75 ± 0.34 mmol/g protein; and the GSH levels were 4.50 ± 1.08, 2.76 ± 0.13, 5.48 ± 0.22, 4.93 ± 0.55 and 6.98 ± 0.33 nmol/g protein in the RI, RIR, RIVR, MRIR and HG groups, respectively. Blood, blood urea nitrogen (BUN) and creatinine levels were classified as high only in the RIR group. The MRIR and RIVR groups, in which oxidative stress was best suppressed, had much milder histopathological and immunohistochemical findings compared to the RIR group. This study has revealed that it is useful to initiate reperfusion of the ischemic tissue with venous blood. PMID:24930510

Cetin, N; Suleyman, H; Sener, E; Demirci, E; Gundogdu, C; Akcay, F

2014-06-01

183

The Effect of Dietary Selenium and Vitamin E on Biochemical Parameters and Survival of Young Among White-Tailed Deer (Odocoileus virginianus)1  

Microsoft Academic Search

Thirty-two adult female white-tailed deer were assigned to four complete pelleted diets (±45ppm vitamin E; ±0.2ppm selenium). Selenium and vitamin E concentration in the unsupplemented diet was 0.04 and 5.5 ppm, respectively. Biochemical parameters of the erythrocyte ( RBC ) glutathione peroxidase system and survival of off-spring to wean ing were followed for 2 years. At the end of the

PAUL S. BRADY; LINDA J. BRADY

184

Dosimetric parameters as predictive factors for biochemical control in patients with higher risk prostate cancer treated with Pd-103 and supplemental beam radiation  

SciTech Connect

Purpose: To analyze the role of dosimetric quality parameters in maximizing cancer eradication in higher risk prostate cancer patients treated with palladium (Pd)-103 and supplemental beam radiation. Methods: One-hundred-seventy-nine patients treated with Pd-103 and supplemental beam radiation, with minimum 2 years follow-up prostate-specific antigen (PSA) values and posttreatment computed tomography scans were analyzed. Dosimetric parameters included the V100 (percent of the postimplant volume covered by the prescription dose), the D90 (the minimum dose that covered 90% of the post implant volume), and the treatment margins (the radial distance between the prostatic edge and the prescription isodose). Treatment margins (TMs) were calculated using premarket software. Results: Freedom from biochemical failure was 79% at 3 years, with 92 of the 179 patients (51%) followed beyond 3 years. In comparing patients who did or did not achieve biochemical control, the most striking differences were in biologic factors of pretreatment PSA and Gleason score. The V100, D90, and average TM all showed nonsignificant trends to higher values in patients with biochemical control. In multivariate analysis of each of the three dosimetric parameters against PSA and Gleason score, TM showed the strongest correlation with biochemical control (p = 0.19). Conclusions: For patients with intermediate and high-risk prostate cancer treated with Pd-103 brachytherapy and external beam radiation, biologic factors (PSA and Gleason score) were the most important determinants of cancer eradication. However, there is a trend to better outcomes among patients with higher quality implant parameters, suggesting that attention to implant quality will maximize the likelihood of cure.

Orio, Peter [Department of Radiation Oncology, University of Washington, Seattle, WA (United States); Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States); Wallner, Kent [Department of Radiation Oncology, University of Washington, Seattle, WA (United States) and Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States) and Group Health Cooperative, Seattle, WA (United States)]. E-mail: kent.Wallner@med.va.gov; Merrick, Gregory [Schiffler Cancer Center, Wheeling, WV (United States); Herstein, Andrew [Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States); Mitsuyama, Paul [Department of Radiation Oncology, University of Washington, Seattle, WA (United States); Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States); Thornton, Ken [Varian Medical Systems, Charlottesville, VA (United States); Butler, Wayne [Schiffler Cancer Center, Wheeling, WV (United States); Sutlief, Steven [Department of Radiation Oncology, University of Washington, Seattle, WA (United States); Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States)

2007-02-01

185

Investigation of MTHFR C677T gene polymorphism, biochemical and clinical parameters in Turkish migraine patients: association with allodynia and fatigue.  

PubMed

We investigated whether there is any relationship between biochemical and clinical parameters of migraine and methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism, associated with the migraine subtypes, symptoms, and gender. A total of 150 migraine patients with and without aura (MA and MO) and 107 non-sufferers were included in the study. Biochemical and clinical parameters were measured and genetic analysis was performed. The MTFHR C677T genotype was significantly higher in the migraine group (p = 0.000). The CT genotype frequency of individuals with a family history of migraine was significantly higher (p = 0.025). This genotype frequency was higher in patients who suffer from compression, allodynia, fatigue, and sleeplessness (p = 0.027, 0.023, 0.006, and 0.05, respectively). Homocysteine and total cholesterol levels were significantly higher in the migraine group than the control group (p = 0.007 and 0.010, respectively). However, the other biochemical and clinical parameters did not differ from each other (p > 0.05), with only attack frequency being significantly higher in the MO group (p = 0.005). While the folate and HDL levels were significantly higher in females (p = 0.001 and 0.000, respectively), the homocysteine and triglyceride levels were significantly higher in males (p = 0.000 for each one). BMIs were significantly lower in the control than the migraine group (p = 0.021); however, an association between the C677T variant and BMI was not found (p = 0.787) in the migraine group. An association between the MTHFR C667T polymorphism and migraine susceptibility was found. Additional studies including genetic, clinic, and biochemical parameters should be conducted to better understand the disease. PMID:23975093

Bahadir, Anzel; Eroz, Recep; Dikici, Suber

2013-11-01

186

Hematological parameters and red blood cell indices in healthy Thai children: a revision for 2005.  

PubMed

In order to provide a reference range for hematological parameters and red blood cells indices in Thai children, we analyzed data from 395 healthy non-anemic Thai children age from 1-16 years old, who all had normal pattern of hemoglobin typing (Hb A and Hb A2 less than 3.5%). Hematological analysis was performed using an automated cell counter and the hemoglobin studies were carried out by electrophoresis and liquid chromatography. Owing to a high frequency of a thalassemia in Thailand, cases with MCV < 75 fL has been excluded from the study since these cases were likely to be heterozygotes for alpha0 thalassemia. These criterions were applied to select so-called 'normal' controls for our analysis. Relatively mild microcytosis and hypochromia were observed, in particular in the first three years of age, suggesting an intrinsic immature nature of erythropiesis in the children. Age-dependent differences in the reference values for white blood cell (WBC) count and differential and platelet count were observed. Herein the hematological data and red blood cell indices were summarized according to ages and these will be of clinically useful for the future reference. PMID:16858855

Viprakasit, Vip; Suwanthol, Lurlugn; Sangpraypan, Tuangrath; Glomglao, Waraporn; Utto, Wiyakan; Veerakul, Gavivann

2005-11-01

187

Iron storage disease in captive Egyptian fruit bats (Rousettus aegyptiacus): relationship of blood iron parameters to hepatic iron concentrations and hepatic histopathology.  

PubMed

This study evaluated the relationship between blood iron parameters and hepatic iron concentrations, and correlation of histologic findings with hepatic iron concentrations in a captive population of Egyptian fruit bats (Rousettus aegyptiacus) and island flying foxes (Pteropus hypomelanus). Blood samples were collected for complete blood counts, plasma biochemical profiles, serum iron concentrations, total iron-binding capacity, whole-blood lead concentrations, and plasma ferritin assays. Liver samples obtained by laparotomy were divided, with one half processed for histologic examination and the other half frozen and submitted for tissue mineral analysis. The histologic sections were scored by two blinded observers for iron deposition, necrosis, and fibrosis. The Egyptian fruit bats had significantly higher liver iron (mean = 3,669 +/- 1,823 ppm) and lead (mean = 8.9 +/- 5.8 ppm) concentrations than the island flying foxes (mean [Fe] = 174 +/- 173 ppm, mean [Pb] = 1.9 +/- 0.5 ppm). Hepatic iron concentrations significantly correlated with tissue lead concentrations, histologic grading for iron and necrosis, serum iron, transferrin saturation, and plasma ferritin (P < 0.001). Blood lead concentrations negatively correlated with tissue lead concentrations (P < 0.001). When the product of transferrin saturation and serum iron was greater than 51, an individual animal had a high probability of having iron overload. When the product of these two variables was greater than 90, there was a high probability that the animal had hemochromatosis. On the basis of this study, it appears that evaluation of serum iron, transferrin saturation, and plasma ferritin are useful and noninvasive methods for diagnosis of hemochromatosis in Egyptian fruit bats. PMID:17323561

Farina, Lisa L; Heard, Darryl J; LeBlanc, Dana M; Hall, Jeffery O; Stevens, Gary; Wellehan, James F X; Detrisac, Carol J

2005-06-01

188

[Effect of anticoagulants on the change of blood parameters in diestric gilts].  

PubMed

The above problem was studied by furnishing four groups of six gilts each with ear catheters for the following intravenous catheter treatment: 2,500 IU heparin per die over ten days, supported by two daily oral applications of 1 g Falithrom, or 2,500 IU heparin plus two daily applications of 1 g Falithrom, all intravenously. The last group remained untreated for control. One day of the dioestrus was chosen for catheter bleeding of all animals at 6 a.m., 8 a.m., noon, and 4 p.m. and subsequent determination from the plasma of free fatty acids, copper, inorganic phosphorus, total protein, albumin, globulin, chloride, urea, cholesterol, alkaline phosphatase, blood sugar, and beta-lipoproteides. Significant differences regarding these parameters between the various groups were not even established, if alpha = 0.25. The anticoagulants used in the study may be used without any reservation for catheter rinsing and clearing and will not cause any significant change in the levels of the blood parameters. PMID:564172

Götze, M; Brüssow, K P

1977-01-01

189

Serum proteins and some biochemical parameters in broiler chickens fed with raw and treated bitter vetch (Vicia ervilia) seeds.  

PubMed

This study carried out to evaluate the effect of bitter vetch seeds on serum proteins and biochemical parameters in broiler chickens. A total of 1320 one-day-old broiler chicks of a commercial breed were placed in 64 pens. Treatments were included raw and four different processed bitter vetch seeds in three levels (150, 300 and 450 g kg(-1)) and a corn-soybean based diet as control. Each treatment group consisted of four replicates. Processing methods were included soaked in water for 12 h, autoclaved, then dried at room temperature (SAD); ground, soaked in water for 24 h, autoclaved and dried (GSAD); ground, soaked in water for 47 h with exchange water every 12 h, cooked and dried (GSCD) and ground, soaked at 1% acetic acid solution for 24 h at 60 degrees C (AA). Feeding raw, AA and GSAD seeds decreased serum albumin significantly (p<0.05) in 21-days-old chicks. Chickens that fed with raw and treated bitter vetch seed had lower alpha 1 and gamma globulins than control (p<0.05). Increasing raw and treated bitter vetch seeds from 15 to 30 and 45% decreased albumin, alpha 1 and gamma globulins and increased alpha 2 and beta globulins significantly (p<0.05). In 14-days-old chicks feeding raw and treated biter vetch had no effect on serum urea, but uric acid concentration decreased significantly (p<0.05). Feeding SAD seeds increased serum urea significantly (p<0.05), but uric acid concentration did not change with feeding raw and treated bitter vetch seeds in 42-day-old chicks. Adding raw and treated bitter vetch seeds to diet increased T4 and decreased T3 concentrations in all ages. At 28-days-old chicks, feeding raw and treated biter vetch seeds decreased alkaline phosphatase concentration significantly than control. Results showed that raw bitter vetch seeds have some toxic effects on metabolism in broiler chickens and GSCD and SAD treatments were more effective to detoxification of this seed. PMID:19069902

Sadeghi, Gh; Pourreza, J

2007-03-15

190

Effect of concentrate level on feeding behavior and rumen and blood parameters in dairy goats: relationships between behavioral and physiological parameters and effect of between-animal variability.  

PubMed

This work aimed first to compare 2 diets differing in their percentage of concentrate [low (LO): 30% concentrate vs. High (HI): 60% concentrate] by measuring simultaneously feeding behavior, rumen parameters, blood and plasma parameters, and milk yield and composition in 8 mid-lactation goats. The second aim was to study the interrelationships between these variables and to analyze the between-animal variability to better understand the between-animal differences in acidosis susceptibility. All of the animals received the 2 diets ad libitum as total mixed ration according to a crossover design of two 4-wk periods. Mean daily DMI was similar between the 2 diets but the variability was higher for the HI than for the LO diet. Goats produced more milk when fed the HI diet compared with the LO diet but with a lower fat:protein ratio (0.81 vs. 0.99). They ate more rapidly the HI than the LO diet but stopped eating sooner after the afternoon feed allowance, and spent less time chewing. The increase in concentrate percentage modified rumen parameters: the pH and acetate:propionate ratio decreased and total VFA, ammonia, and soluble carbohydrate concentrations increased. Hematocrit, plasma NEFA, and blood K and Ca concentrations decreased but glycemia and uremia increased. Other parameters were not modified: milk fat content, blood pH, and bicarbonate and Na concentrations. A large between-animal variability was detected for all the measured parameters, especially for feeding behavior, with important consequences on rumen and blood parameters. This work confirmed the effects of a high percentage of concentrate on feeding behavior, rumen and blood parameters, and milk production, and some known relationships such as the positive link between rumen pH and chewing index. It also pointed out other relationships between parameters seldom measured at the same time, such as rumen redox potential or blood pH and chewing index, or the negative link between blood and rumen pH. When the animals spent a lot of time chewing, they probably produced a lot of saliva that buffered the rumen pH and prevented them from suffering from subacute ruminal acidosis. However, they used part of their blood bicarbonates reserve, which might have induced metabolic acidosis, as rumen and blood pH were inversely related. This could explain why some animals suffer from acidosis and others do not in a herd receiving the same diet, and why some animals seem to suffer more from subacute ruminal acidosis and others from metabolic acidosis. PMID:24952476

Giger-Reverdin, S; Rigalma, K; Desnoyers, M; Sauvant, D; Duvaux-Ponter, C

2014-07-01

191

Detecting different levels of drought stress in apple trees ( Malus domestica Borkh.) with selected biochemical and physiological parameters  

Microsoft Academic Search

Rational irrigation scheduling based on sensing drought stress directly in plants is becoming more important due to increasing worldwide scarcity of fresh water supplies. In order to evaluate a set of potential biochemical and physiological stress indicators and select the best drought stress markers in apple trees, two experiments with potted trees and an experiment with intensive orchard grown apple

Helena Šircelj; Michael Tausz; Dieter Grill; Franc Bati?

2007-01-01

192

Biochemical differentiation of the porphyrias  

Microsoft Academic Search

Objectives: To differentiate the porphyrias by clinical and biochemical methods.Design and methods: We describe levels of blood, urine, and fecal porphyrins and their precursors in the porphyrias and present an algorithm for their biochemical differentiation. Diagnoses were established using clinical and biochemical data. Porphyrin analyses were performed by high performance liquid chromatography.Results and conclusions: Plasma and urine porphyrin patterns were

J. Thomas Hindmarsh; Linda Oliveras; Donald C. Greenway

1999-01-01

193

Biochemical and histopathological studies to assess chronic toxicity of triazophos in blood, liver and brain tissue of rats  

Microsoft Academic Search

Triazophos, O,O-diethyl-1-H-1,2,4-triazol-3-yl phosphorothioate, (TZ) is an organophosphorus pesticide which is extensively used in agriculture for controlling insect pests. Except a FAO\\/WHO report no study has investigated its short-term toxicity with respect to its potential to cause biochemical and histopathological alterations. The present study was designed to identify the effect of TZ at different doses (1.64, 3.2 and 8.2mg\\/kg) on the

Smita Jain; Rafat S. Ahmed; Vinod Kumar Arora; Basu Dev Banerjee

2011-01-01

194

Serial blood T cell repertoire alterations in multiple sclerosis patients; correlation with clinical and MRI parameters.  

PubMed

A significant skewing of the peripheral T cell repertoire has been shown in relapsing-remitting multiple sclerosis (MS). Most of the studies already performed in this field are cross-sectional and therefore, little is known of the T cell repertoire evolution over time in MS and the correlation of T cell repertoire variation with clinical and MRI parameters. This study was performed on serially harvested frozen PBMC from nine untreated MS patients (27 samples) and 14 healthy individuals. The blood T cell repertoire of each patient was analysed at the complementarity determining region 3 (CDR3) level and compared with a monthly MRI scan performed over a six month period with assessment of T2 lesion load and gadolinium enhancing lesions. A highly significant blood T cell repertoire skewing was observed in MS patients as compared with healthy controls (p<0.01). In addition, the number of altered Vbeta families correlated significantly with both the T2 lesion volume and the number of gadolinium enhancing lesions as assessed by MRI (Spearman correlation tests, r=0.51 and r=0.44, p<0.01 and p<0.05 respectively). Furthermore, the variation of the number of altered Vbeta families over time also correlated with the appearance of new gadolinium enhancing lesions (r=0.36, p=0.05). These findings which need confirmation on larger serial cohorts, suggest an association between the magnitude of TCRBV CDR3 length distribution alterations in the peripheral blood of MS patients and the disease process. PMID:16806500

Laplaud, David-Axel; Berthelot, Laureline; Miqueu, Patrick; Bourcier, Kasia; Moynard, Julien; Oudinet, Yannick; Guillet, Marina; Ruiz, Catherine; Oden, Neal; Brouard, Sophie; Guttmann, Charles R G; Weiner, Howard L; Khoury, Samia J; Soulillou, Jean-Paul

2006-08-01

195

Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat  

PubMed Central

Background The purpose of this study was to investigate the effect of acute dosing with silver nanoparticles (AgNPs) and identify potential ultrastructural alterations in the liver and kidney and their effect on blood parameters in the albino rat. Methods Twenty rats were used to assess the acute effects of AgNPs. Rats in the treatment group were injected intraperitoneally with 0.5 mL of distilled water containing AgNPs at a dose of 2,000 mg/kg body weight followed by a second injection after 48 hours. Control rats received two 0.5 mL doses of distilled water only. After 3 days, blood samples were collected, and the rat kidneys and livers were extracted and processed for electron microscopy to investigate for hematologic and histopathologic alterations. Results Renal tubules showed swollen epithelium with cytoplasmic vacuolization, thickening of the basement membrane, and destruction of some mitochondrial cristae. Podocytes showed elongation and swelling of their primary and secondary processes. The basement membrane of the capillary tufts became thicker. The hepatic tissue showed narrowing of the sinusoids, swollen hepatocytes with hypertrophied nucleoli, and accumulation of fat globules in the nucleoplasm and cytoplasm. The hepatic sinusoids showed hypertrophied endothelial and Kupffer. Destructed cristae of some mitochondria, endosomes, and larger lysosomes filled with Ag-NPs were also observed in the Kupffer cells. Significant increases were observed in white blood cell count, lymphocyte count, granulocytes, and hemoglobin. There was a significant increase in serum creatinine, urea, and aspartate and alanine aminotransferases. Conclusion To the best of the authors’ knowledge, the ultrastructural changes in renal and liver tissue observed in this study have not been described before. Our results suggest that injection of AgNPs could have severe cytotoxic effects on the structure and function of these organs. PMID:24711700

Sarhan, Osama Mohamed M; Hussein, Rehab M

2014-01-01

196

An experimental soft-release of oil-spill rehabilitated American coots (Fulica americana): II. Effects on health and blood parameters.  

PubMed

The Unocal-Metrolink oil spill of 21 February 1995 resulted in approximately 7800 barrels of San Joaquin crude oil being deposited into the San Gabriel River in Huntington Beach, CA, USA. In order to determine long-term pathological effects of oil exposure and rehabilitation, hematological and serum biochemical parameters for both rehabilitated (RHB) American coots (Fulica americana) and reference (REF) coots were examined every 3-4 weeks (56, 81, 108 and 140 days post oil exposure) after birds were cleaned, rehabilitated and soft-released. Most significant differences in monthly comparisons between RHB and REF birds occurred 56 days following oil exposure. Total white blood cell (WBC) count, albumin:globulin (A:G) ratio and calcium concentration were higher in RHB birds compared to REF birds 56 days post oil exposure. In addition, mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase and creatine kinase activities, and creatinine, total protein (TP) and globulin concentrations were lower in RHB birds. Blood results from 56 days post oil exposure for RHB coots which subsequently died were compared to blood results from days 108 and 140 for REF coots which survived. Oiled and rehabilitated birds which died had significantly higher WBCs, packed cell volume, TP and globulin concentrations, and lower A:G ratio, MCH, MCHC, glucose and sodium concentrations compared to REF birds which survived. Blood result differences detected at 3-4-week intervals between RHB and REF survivors, and differences detected between RHB coots which died and REF coots which survived, suggested that RHB coots developed an inflammatory response (infectious or non-septic) and, concurrently, may have experienced decreased immune responsiveness. Additionally, RHB coots experienced either an iron (Fe) utilization or Fe metabolism problem. These pathophysiological mechanisms were consistent with increased hemosiderin (stored Fe) present in the liver, spleen and kidney of necropsied RHB birds, and may have contributed to RHB coot mortality. When blood parameter differences were examined for their impact on survival time, it was determined that RHB coots had shorter survival times if they had very high cholesterol (> or =449 mg/dl) or chloride (> or =110 MEQ/l) concentrations on day 56 post oil exposure. Interestingly, the lack of differences between RHB and REF coots from day 81 through day 140 suggested that, from a hematologic and clinical chemistry perspective, coots which were oiled, rehabilitated, released and survived at least 3.5 months could not be differentiated from wild (REF) coots. From these findings it appears that blood analysis, coupled with post-release survival data, may help discern reasons for increased mortality of oiled and rehabilitated birds, compared to non-oiled reference birds. PMID:15092975

Newman, S H; Anderson, D W; Ziccardi, M H; Trupkiewicz, J G; Tseng, F S; Christopher, M M; Zinkl, J G

2000-03-01

197

Hemodynamic and Arterial Blood Gas Parameters during Cemented Hip Hemiarthroplasty in Elderly Patients  

PubMed Central

Background: Patients undergoing cemented hip hemiarthroplasty may develop bone cement implantation syndrome (BCIS) which is a leading cause of intraoperative complications. The purpose of this study was to evaluate cardiovascular changes during cemented hip hemiarthroplasty in elderly patients. Methods: Cemented hip hemiarthroplasty was performed on 72 patients with femoral neck fracture. All patients were catheterized with a radial artery catheter to assess mean arterial pressure (MAP) and arterial blood gas (ABG) in these time points: just before cementation, just after cementation (0th), 5 min (5th) and 10 min (10th) after cementation, and at the end of surgery (END). Also, systolic and diastolic blood pressure (SBP & DBP), heart rate and any arrhythmia or cardiac arrest was evaluated. Results: Seventy-two patients (33 females, 39 males; mean age: 66.8±7 years) were evaluated. All parameters changed during cementation with a significant drop in MAP, SBP, and DBP immediately after cementation and pH and base excess decreased significantly (P<0.001) with no changes in O2 saturation. Mean heart rate rose until the 5th and then decreased dramatically with no bradycardia presentation. During cementation, 12 patients showed arrhythmia, but no cardiac-arrest was observed. Conclusions: Under strict observation of a anesthesiology care team, hemiarthroplasty can be a safe method for femoral neck fracture in elderly osteoporotic patients without severe cardiopulmonary compromise. PMID:25386576

Soleimanha, Mehran; Sedighinejad, Abbas; Haghighi, Mohammad; Nabi, Bahram Naderi; Mirbolook, Ahmad Reza; Mardani-Kivi, Mohsen

2014-01-01

198

Interactive effects of photoperiod and light intensity on blood physiological and biochemical reactions of broilers grown to heavy weights  

Technology Transfer Automated Retrieval System (TEKTRAN)

The effects of photoperiod, light intensity, and their interaction on blood acid-base balance, metabolites, and electrolytes in broiler chickens under environmentally controlled conditions were examined in 2 trials. The experiment was consisted of a factorial arrangement of treatments in a randomize...

199

Influence of gene variants related to calcium homeostasis on biochemical parameters of women with polycystic ovary syndrome  

Microsoft Academic Search

Purpose  The purpose of this study was to evaluate the associations between polymorphisms in vitamin D receptor (VDR), parathyroid\\u000a hormone (PTH), calcium sensing receptor (CASR), insulin receptor (INSR), and adiponectin (ADIPOQ) genes and biochemical characteristics\\u000a of women with polycystic ovary syndrome (PCOS).\\u000a \\u000a \\u000a \\u000a \\u000a Methods  Serum levels of LH, FSH, estradiol, testosterone, prolactin, SHBG, glucose, IGF-1, IGFBP-1, calcium, phosphorus, PTH, 25(OH)D,\\u000a and 1,25(OH)2 D

Fariba Ranjzad; Aidin Mahban; Atena Irani Shemirani; Touraj Mahmoudi; Mohsen Vahedi; Abdolrahim Nikzamir; Mohammad Reza Zali

2011-01-01

200

[The effects of grape seed and coriander oil on biochemical parameters of oral fluid in patients with periodontitis].  

PubMed

Biochemical changes that are taking place in patients with periodontitis 1 and 2 the quality of the individuals place in a laqtatdegidrogenazis, alkaline phosphatase activity increase and reduced activity amilazais. Therefore we can conclude that, there is an increase in superoxide substances, which causes an increase in the oral cavity patobakteriebis and quality of periodontitis. The grape seed and coriander oil is of vegetable origin and antioxidant drugs. Their action causes a statistically significant increase in the amilazis, alkaline phosphatase and laqtatdegidrogenazis reduction, while the latter leads pH - rate of return to oral fluid. It should be noted that the positive effect of coriander oil, but less effective. PMID:25416221

Nikolaishvili, M; Gogua, M; Franchuki, Q; Tufinashvili, T; Zurabashvili, D

2014-10-01

201

High-Moisture Diet for Laboratory Rats: Complete Blood Counts, Serum Biochemical Values, and Intestinal Enzyme Activity  

NASA Technical Reports Server (NTRS)

Rats were fed an irradiated high-moisture diet (KSC-25) with or without access to a water bottle. Physiologic values were compared between these two groups and a group of rats fed a purified diet. Hematologic and serum biochemical values, urine specific gravity, and intestinal enzyme activities were determined from samples collected from the three groups of rats. Sprague Dawley rats (n=32) fed the irradiated high-moisture diet with or without a water bottle were the test animals. Rats (n=16) fed an irradiated purified diet and water provided via a water bottle were the control group. The purified diet formulation, modified AIN-76A, is a commonly used purified diet for laboratory rodents. All rats remained alert and healthy throughout the study. A comparison of the physiologic values of rats in this study with reported normal values indicated that all of the rats in the study were in good health. Significant differences (P less than 0.05) of the physiologic values from each rat group are reported.

Battles, August H.; Knapka, Joseph T.; Stevens, Bruce R.; Lewis, Laura; Lang, Marie T.; Gruendel, Douglas J.

1991-01-01

202

Metabolism and biochemical/physiological roles of chondroitin sulfates: analysis of endogenous and supplemental chondroitin sulfates in blood circulation.  

PubMed

Chondroitin sulfate (CS) is a linear heteropolysaccharide consisting of repeating disaccharide units of glucuronic acid and galactosamine, which is commonly sulfated at C-4 and/or C-6 of galactosamine. The administration of CS as a supplement or a drug for the treatment of osteoarthrosis, the prevention of subsequent coronary events, treatment of psoriasis and ophthalmic diseases has been suggested. Much debate on the metabolism of CS and therefore the effectiveness of these treatments, especially after oral administration, has arisen due to the macromolecular nature of CS. Difficulties in analysing CS in blood due to the low endogenous concentrations and the covalent and anionic complexes with proteins have hampered the resolution of these issues. In this review, the information on the pharmacokinetics of CS obtained from studies in experimental animals and in humans is presented. Emphasis has been given to the analytical methods used for the determination of glycosaminoglycans, intact CS and CS-derived disaccharides in blood serum and plasma. PMID:16779785

Lamari, Fotini N; Theocharis, Achilleas D; Asimakopoulou, Athanasia P; Malavaki, Christina J; Karamanos, Nikos K

2006-01-01

203

Survey of selected pathogens and blood parameters of northern yellowstone elk: Wolf sanitation effect implications  

USGS Publications Warehouse

The restoration or conservation of predators could reduce seroprevalences of certain diseases in prey if predation selectively removes animals exhibiting clinical signs. We assessed disease seroprevalences and blood parameters of 115 adult female elk (Cervus elaphus) wintering on the northern range of Yellowstone National Park [YNP] during 2000-2005 and compared them to data collected prior to wolf (Canis lupus) restoration (WR) in 1995 and to two other herds in Montana to assess this prediction. Blood parameters were generally within two standard deviations of the means observed in other Montana herds (Gravelly-Snowcrest [GS] and Garnet Mountain [GM]), but Yellowstone elk had higher seroprevalences of parainfluenza-3 virus (95% CI YNP = 61.1-78.6, GS = 30.3-46.5) and bovine-virus-diarrhea virus type 1 (95% CI YNP = 15.9-31.9, GM = 0). In comparisons between pre-wolf restoration [pre-WR] (i.e., prior to 1995) seroprevalences with those post-wolf restoration [post-WR] in Yellowstone, we found lower seroprevalences for some disease-causing agents post-wolf restoration (e.g., bovine-virus-diarrhea virus type-1 [95% CI pre-WR = 73.1-86.3, post-WR = 15.9-31.9] and bovine-respiratory syncytial virus [95% CI pre-WR = 70.0-83.8, post-WR = 0]), but similar (e.g., Brucella abortus [95% CI pre-WR = 0-4.45, post-WR = 0-4.74] and epizootic hemorrhagic disease virus [95% CI pre-WR = 0, post-WR = 0]) or higher for others (e.g., Anaplasma marginale [95% CI pre-WR = 0, post-WR = 18.5-38.7] and Leptospira spp. [95% CI pre-WR = 0.5-6.5, post-WR = 9.5-23.5]). Though we did not detect an overall strong predation effect through reduced disease seroprevalence using retrospective comparisons with sparse data, our reference values will facilitate future assessments of this issue.

Barber-Meyer, S. M.; White, P.J.; Mech, L.D.

2007-01-01

204

Figure Legend Biochemical parameters in BHcB mice. Blood was collected after a 4h fast  

E-print Network

.001 female 0.15 (0.04) 0.27 (0.03) * calculated by 2-way ANOVA #12;Table S3: Serum Insulin and Glucagon Insulin (±SEM) p-value Glucagon (±SEM) p-value (pmol/L) (ng/L) C3H 117 (24) 47.1 (3.6) HcB-19 148 (29) 0.6) bTKO 63 (8) 0.672 59.6 (2.1) 0.749 Serum insulin and glucagon concentrations were measured after a 4

Attie, Alan D.

205

Effects of acute exposure to metribuzin on some hematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.).  

PubMed

The aim of the study was to evaluate acute toxic effects of the preparation Sencor 70 WG (metribuzin 70% W/V) on hematological, biochemical indices and histology of the common carp (Cyprinus carpio L.). In carp exposed for 96 h to Sencor 70 WG in the concentration of 250.2 mg/L, showed significantly lower (p<0.01) values of plasma total proteins, albumins, total globulins, triacylglycerols, lactate dehydrogenase, lactate, inorganic phosphate, hematocrit, hemoglobin concentration, mean erythrocyte volume, the leucocrite value, lymphocyte, and significantly higher (p<0.01) values of glucose, ammonia, calcium, monocytes, neutrophile granulocytes, developmental forms myeloid sequence and basophiles compared to the control group. Histopathological examination revealed hyaline degeneration of the epithelial cells of renal tubules of the caudal kidney. This alteration of kidney resulted in hypoproteinemia, followed by generation of transudate in body cavity. PMID:19159050

Velisek, J; Svobodova, Z; Piackova, V; Sudova, E

2009-04-01

206

Dissection of the cord blood stromal component reveals predictive parameters for culture outcome.  

PubMed

In regenerative medicine, human cord blood-derived multipotent mesenchymal stromal cells (CBMSCs) stand out for their biological peculiarities demonstrated in in vitro and in vivo preclinical studies. Here, we present our 9-year experience for the consistent isolation of CBMSCs. Although nearly one CB unit out of two retains the potential to give rise to MSC colonies, only 46% of them can be cultured till low passages (P?4), but one-fourth of those reaches even higher passages (P?8). Subsequent characterization for morphological, clonal, differentiation, and proliferation properties revealed two divergent CBMSC behaviors. In particular, a cumulative population doublings cut-off (CPD=15) was identified that undoubtedly distinguishes two growth curves, and different degrees of commitment toward osteogenesis were observed. These data clearly show the existence of at least two distinct CBMSC subsets: one mainly short-living and less proliferative (SL-CBMSCs), the other long-living, with higher growth rate, and, very importantly, with significantly (P?0.01) longer telomere (LL-CBMSCs). Moreover, significant differences in the immunoprofile before seeding were found among CB units giving rise to LL-CBMSCs or SL-CBMSCs or showing no colony formation. Finally, all the aforementioned results provided a peculiar and useful set of parameters potentially predictive for CBMSC culture outcome. PMID:25046283

Barilani, Mario; Lavazza, Cristiana; Viganò, Mariele; Montemurro, Tiziana; Boldrin, Valentina; Parazzi, Valentina; Montelatici, Elisa; Crosti, Mariacristina; Moro, Monica; Giordano, Rosaria; Lazzari, Lorenza

2015-01-01

207

Influence of repeated subcutaneous G-CSF injections on selected blood parameters relevant for monitoring programmes in sports drug testing.  

PubMed

The use of growth factors in sports is restricted under the terms of the World Anti-Doping Code (WADC). While the beneficial effects of erythropoietin (EPO) on erythropoiesis and therefore its performance-enhancing properties have been well documented and established for decades, the aim of this study was to elucidate the relevance of the cytokine G-CSF in a doping control context, particularly concerning its influence on selected blood parameters representing central aspects of the Athlete Biological Passport. For that purpose, the effect of repeated subcutaneous granulocyte colony-stimulating factor (G-CSF) injections in therapeutic dosages (10 µg/kg/d) on white blood cells, erythrocytes, hemoglobin, hematocrit and percent reticulocytes was analyzed by using commonly employed fluorescence flow cytometry-based approaches. A total of 20 people were tested (14 male, 6 female) and both white blood cell count and reticulocyte percentages were found to significantly increase following a 5-day treatment with G-CSF. Simultaneously, all other volume-dependent parameters (red blood cell count, hemoglobin, hematocrit) slightly but significantly decreased. Due to the relevance of these measurands for the validity of blood tests for doping controls and the anecdotal evidence of G-CSF being potentially misused by elite athletes, G-CSF analyses might be indicated in case of unusually altered blood profiles. PMID:23047806

Walpurgis, Katja; Slijepcevic, Mirjana; Wenzel, Folker; Thomas, Andreas; Geyer, Hans; Franz, Stefan; Schänzer, Wilhelm; Thevis, Mario

2012-10-01

208

Effect of feeding of calcium hydroxide-treated or vitamin E-supplemented cottonseed meal on plasma gossypol levels, blood parameters, and performance of Bikaneri lambs.  

PubMed

To study the effect of feeding calcium hydroxide-treated or vitamin E-supplemented cottonseed meal (CSM) incorporated diets on plasma gossypol, blood parameters and animal performance, 24 male Bikaneri lambs of 6-7 months of age and of uniform body weight were divided into four groups of six animals each in a completely randomized design and respectively fed isonitrogenous and isocaloric concentrate mixtures containing 20% soybean meal (CON) or 40% raw CSM (RCSM), 40% raw CSM supplemented with 500 IU of vitamin E per head per day (ERCSM), and 40%, 1.5% calcium hydroxide-treated CSM (CaCSM) along with ad libitum wheat straw throughout 510 days of experimental feeding. The lambs on all the diets grew linearly throughout the experimental period. The total weight gain, in turn the average daily gain (ADG), was not affected by dietary variations. The daily intake of dry matter, crude protein (CP), digestible crude protein (DCP) and total digestible nutrients (TDN) were found comparable among lambs of all the groups. Though total gossypol intake was similar in RCSM, ECSM and CaCSM groups, however, free gossypol intake was significantly higher (P < 0.01) in RCSM, ECSM groups as compared to CaCSM group. Serum iron and blood hemoglobin levels were significantly (P < 0.05) lower in RCSM group as compared to CaCSM and CON groups, and ALT activity was significantly (P < 0.05) higher on RCSM group as compared to other groups. Plasma gossypol and osmotic fragility of erythrocytes were significantly (P < 0.05) increased in RCSM group as compared to CaCSM and ERCSM groups. However, there was no significant difference in the concentration of other blood/serum biochemical constituents among the lambs on different groups. Based on the results, it may be concluded that feeding of 40% CSM in the concentrate mixture of the diet in Bikaneri lambs did not have any major adverse effect in blood parameters and animal performance. Either calcium hydroxide treatment or vitamin E supplementation did not produce any major additional benefits. PMID:23345064

Kannan, Alagarsamy; Sastry, Vadali Rama Bhadra; Agrawal, Dinesh Kumar; Kumar, Avneesh

2013-08-01

209

Influence of Dietary Glutamine Supplementation on Growth Performance, Small Intestinal Morphology, Immune Response and Some Blood Parameters of Broiler Chickens  

Microsoft Academic Search

The objective of this experiment was to evaluate the influence of glutamine (Gln) supplementation in the diet of broiler chickens on the growth performance, immune response as well as some blood parameters. Two hundred fifty one day old broiler chickens were allotted into five equal groups (50 chicks per each) of mixed sex. Five experimental diets were formulated to be

M. A. Soltan

2009-01-01

210

Performance, Blood Parameters, and Fecal Egg Counts When Meat Goats Were Finished on Alfalfa, Red Clover, or Orchardgrass Pastures  

Technology Transfer Automated Retrieval System (TEKTRAN)

In the Appalachian Region of the USA, meat goat industries are growing rapidly on small farms to help produce meats for ethnic markets. This experiment was conducted to evaluate weight gain, blood parameters (measurements of nutrient use and anemia resulting from infection with the GI parasite Haem...

211

Effect of Dietary Anise Seeds Supplementation on Growth Performance, Immune Response, Carcass Traits and Some Blood Parameters of Broiler Chickens  

Microsoft Academic Search

The objective of the present study was to investigate the effect of dietary anise seeds supplementation on growth performance, immune response, some blood parameters and carcass traits of broiler chickens. Two hundred and seventeen Arbor Acre one day old broiler chicks were randomly allotted into 7 groups (31 per each) of mixed sex. Anise seed was supplemented to the basal

M. A. Soltan; R. S. Shewita; M. I. El-Katcha

2008-01-01

212

Restrictive versus liberal blood transfusion policy for hepatectomies in cirrhotic patients  

Microsoft Academic Search

To evaluate the worth of intra- and postoperative blood transfusion in cirrhotic patients undergoing resection for hepatocellular carcinoma, we compared 13 patients receiving transfusions and 14 matched contemporary patients who did not receive blood. Preoperative hematological and biochemical parameters, the type and extent of liver resection, and the mean blood loss (862 and 870 ml) were similar in the 2

Masatoshi Makuuchi; Tadatoshi Takayama; Peter Gunvén; Tomoo Kosuge; Susumu Yamazaki; Hiroshi Hasegawa

1989-01-01

213

Identification of ticks and detection of blood protozoa in friesian cattle by polmerase chain reacton test and estimation of blood parameters in district Kasur, Pakistan.  

PubMed

The study was carried out to detect Theileria annulata, the causative agent of theileriosis, and Babesia bovis, the causative agent for babesiosis, in Friesian cattle by PCR and conventional blood smear examination. One hundred blood samples obtained from diseased Friesian cattle kept on private livestock farms at Pattoki, District Kasur, Pakistan were collected in addition to 20 blood samples obtained from non-diseased animals. The disease manifestations observed clinically included high fever, swelling of sub mandibular and sub scapular lymph nodes, weakness, increased respiration and pulse, anorexia, loss of condition and rough hair coat. Neurologic sign of in coordination was also seen in weak animals. Signs of lacrimation, pale conjunctiva, diarrhoea, dyspnea and frothy nasal discharge were observed in only one animal. Clinically nine animals showed signs of haemoglobinuria. Diagnosis of bovine theileria and babesia species was based on finding many intraerythrocytic piroplasms of both blood protozoa with clinical signs associated with anaemia, lymph node hyperplasia and haemoglobinuria. One hundred samples of ticks were also collected for identification of vector. Results showed that the prevalence of Hyalomma tick was highest (15%) followed by Boophilus (12%), Haemaphysalis (5%) and Rhipicephalus (3%). The blood smear examination showed 21% (21/100) samples positive for blood parasites out of which 66.6% (14/ 21) samples were positive for theileriosis while 42.8% (9/21) were positive for babesiosis. It was also recorded that 66.66% (6/9) samples were positive for B.bigemina while 33.33% (3/9) were positive for B.bovis. The results showed that 60% (60/100) samples were positive for blood parasites by PCR test. Out of these 60% (36/60) were positive for T.annulata while 33.33% (20/60) were positive for babesia. The specificity and sensitivity of PCR test was higher than blood smear examination. The blood parameters in haemoparasites infection were also analyzed and the results showed significant decrease in total erythrocyte count and haemoglobin while MCV, MCH values increased and MCHC was slightly less than normal indicating macrocytic hypochromic anaemia. PMID:18575972

Durrani, A Z; Kamal, N

2008-08-01

214

Blood Flow Parameters of the Central Retinal and Internal Carotid Arteries in Asymmetric Diabetic Retinopathy  

PubMed Central

Purpose To investigate the correlation between diabetic retinopathy severity and blood flow parameters in the central retinal artery (CRA) and internal carotid artery (ICA). Methods This comparative study included 40 eyes of 20 patients with asymmetric diabetic retinopathy. Twelve subjects had asymmetric diabetic macular edema while eight patients had proliferative diabetic retinopathy (PDR) in one eye and non-proliferative diabetic retinopathy (NPDR) in the fellow eye. Bilateral color Doppler imaging (CDI) of the CRA and ICA was performed to determine resistance index (RI) and peak systolic velocity (PSV). RI and PSV values were compared between eyes with higher grades of macular edema (> 2 grades of difference with the fellow eye) and fellow eyes with less severe macular edema, as well as between eyes with PDR and fellow eyes with NPDR. Results Mean RI of the CRA in eyes with higher grades of macular edema was 0.78±0.11 as compared to 0.69±0.08 in fellow eyes with less severe macular edema (P=0.012), while PSV of the CRA was 58.83±18.93 cm/s in eyes with higher grades of macular edema versus 59.75±11.83 cm/s in fellow eyes with less severe macular edema (P=1.00). Mean PSV of the ICA was 55±23.94 cm/s in eyes with PDR and 69.25±34.30 cm/s in eyes with NPDR (P=0.008) while mean RI of the ICA was 0.81±0.13 in eyes with PDR and 0.76±0.12 in eyes with NPDR (P=1.00). Conclusion Evaluation of RI in the CRA and PSV in the ICA by CDI in diabetic patients may identify eyes at risk of more severe macular edema and PDR. PMID:23503081

Mehdizadeh, Morteza; Lotfi, Mehrzad; Ghoddusi Johari, Hamed; Ghassemifar, Vahid; Afarid, Mehrdad

2012-01-01

215

EFFECTS OF LOW LEVEL CARBON MONOXIDE EXPOSURE. BLOOD LIPIDS AND COAGULATION PARAMETERS  

EPA Science Inventory

The study examined the effects of carbon monoxide (CO) in 50 and 100 ppm doses on response to treadmill exercise, blood coagulation and blood lipids in normal men. Twenty-three men were exposed to CO or to air in a double-blind protocol. After exposure, each underwent a graded ex...

216

Effect of the treatment with Achyrocline satureioides (free and nanocapsules essential oil) and diminazene aceturate on hematological and biochemical parameters in rats infected by Trypanosoma evansi.  

PubMed

This study aimed to verify the effect of the treatment with A.?satureioides essential oil (free and nanoencapsulated forms) and diminazene aceturate on hematological and biochemical variables in rats infected by Trypanosoma evansi. The 56 rats were divided into seven groups with eight rats each. Groups A, C and D were composed by uninfected animals, and groups B, E, F and G were formed by infected rats with T.?evansi. Rats from groups A and B were used as negative and positive control, respectively. Rats from the groups C and E were treated with A.?satureioides essential oil, and groups D and F were treated with A.?satureioides nanoencapsulated essential oil. Groups C, D, E and F received one dose of oil (1.5?mL?kg(-1)) during five consecutive days orally. Group G was treated with diminazene aceturate (D.A.) in therapeutic dose (3.5?mg?kg(-1)) in an only dose. The blood samples were collected on day 5 PI for analyses of hematological (erythrocytes and leukocytes count, hemoglobin concentration, hematocrit, mean corpuscular and mean corpuscular hemoglobin concentration) and biochemical (glucose, triglycerides, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin, urea and creatinine) variables. A.?satureioides administered was able to maintain low parasitemia, mainly the nanoencapsulated form, on 5 days post infection. On the infected animals with T.?evansi treated with A.?satureioides essential oil (free and nanocapsules) the number of total leucocytes, lymphocytes and monocytes present was similar to uninfected rats, and different from infected and not-treated animals (leukocytosis). Treatment with A.?satureioides in free form elevated levels of ALT and AST, demonstrating liver damage; however, treatment with nanoencapsulated form did not cause elevation of these enzymes. Finally, treatments inhibited the increase in creatinine levels caused by infection for T.?evansi. In summary, the nanoencapsulated form showed better activity on the trypanosome; it did not cause liver toxicity and prevented renal damage. PMID:25499512

Do Carmo, Guilherme M; Baldissera, Matheus D; Vaucher, Rodrigo A; Rech, Virginia Cielo; Oliveira, Camila B; Sagrillo, Michele Rorato; Boligon, Aline A; Athayde, Margareth L; Alves, Marta P; França, Raqueli T; Lopes, Sonia T A; Schwertz, Claiton I; Mendes, Ricardo E; Monteiro, Silvia G; Da Silva, Aleksandro S

2015-02-01

217

The clinical spectrum of renal osteodystrophy in 57 chronic hemodialysis patients: a correlation between biochemical parameters and bone pathology findings.  

PubMed

Fifty-nine chronic hemodialysis patients who had been on dialysis for an average of 77 months underwent bone biopsies and the pathologic findings were correlated with biochemical and demographic data. All but two had evidence of renal osteodystrophy, 23 with osteitis fibrosa (OF), 19 with osteomalacia and/or adynamic disease (OM/AD), and 15 with mixed osteodystrophy (MOD). Patients in each group were similar with regard to age, sex distribution, duration of dialysis, unstimulated serum aluminum, calcium and phosphorus. Patients with osteitis fibrosa (OF) had statistically higher DFO stimulated aluminum, alkaline phosphatase and PTHC levels than the other two groups although there was marked individual variation. The bone biopsies were also evaluated for the amount of aluminum deposited in the osteoid seam. All 23 of the patients with OF and 11 of the 15 patients with MOD had no, mild, or minimal aluminum deposition but 12 of the 19 patients with OM/AD had moderate to marked aluminum deposition. Patients with minimal to mild aluminum deposition were similar in age, duration of dialysis, sex distribution, unstimulated and DFO stimulated aluminum levels, calcium, phosphorus, alkaline phosphatase to those with moderate to marked deposition but had significantly higher parathormone levels. All patients had been treated in a similar fashion regarding diet, oral phosphate binders and vitamin D; therefore, the observed differences in bone pathology were not readily explicable. However, patients who were found to have osteitis fibrosa and those with minimal to mild aluminum deposition had significantly higher parathormone levels when compared with patients in the other groups at the inception of dialysis. PMID:2019018

Chazan, J A; Libbey, N P; London, M R; Pono, L; Abuelo, J G

1991-02-01

218

Comparison of the effects of lanthanum, cerium and praseodymium on rumen fermentation, nutrient digestion and plasma biochemical parameters in beef cattle.  

PubMed

The objectives of the trial were to compare the effects of supplementing rare earth elements (REE) lanthanum (La), cerium (Ce) and praseodymium (Pr) on rumen fermentation, nutrient digestion, methane (CH4) production, nitrogen (N) balance and plasma biochemical parameters in beef cattle. Four Simmental male cattle, aged 12 months, with initial average liveweight of 333 ± 9 kg and fitted with rumen cannulas, were fed with a basal ration composed of concentrate mixture and maize silage. Animals received a basal ration without adding REE (Control) or three treatments, i.e. supplementing LaCl3, CeCl3 or PrCl3 at 204 mg/kg DM to the basal ration, respectively, which were allocated in a 4 × 4 Latin square design. Each experimental period lasted 15 d, consisting of 12 d for pre-treatment and three subsequent days for sampling. Results showed that all tested levels of REE tended to increase neutral detergent fibre digestibility (p = 0.064) and tended to decrease rumen CH4 production (p = 0.056). Supplementing LaCl3 and CeCl3 decreased total N excretion and urinary N excretion, increased N retention (p < 0.05), tended to increase total urinary purine derivatives (PD) (p = 0.053) and microbial N flow (p = 0.095), whereas supplementing PrCl3 did not affect N retention, urinary PD and microbial N flow. No differences were found in the effects of nutrient digestibility, CH4 production and plasma biochemical parameters among LaCl3, CeCl3 and PrCl3. Further trials using graded levels of LaCl3, CeCl3 and PrCl3 in a wide range are needed to obtain more pronounced results for comparing effects of La, Ce and Pr on rumen fermentation and nutrient digestion in beef cattle. PMID:25575216

Lin, Shixin; Wei, Chen; Zhao, Guangyong; Zhang, Tingting; Yang, Kai

2015-02-01

219

A therapy parameter-based model for predicting blood glucose concentrations in patients with type 1 diabetes.  

PubMed

In this paper, the problem of predicting blood glucose concentrations (BG) for the treatment of patients with type 1 diabetes, is addressed. Predicting BG is of very high importance as most treatments, which consist in exogenous insulin injections, rely on the availability of BG predictions. Many models that can be used for predicting BG are available in the literature. However, it is widely admitted that it is almost impossible to perfectly model blood glucose dynamics while still being able to identify model parameters using only blood glucose measurements. The main contribution of this work is to propose a simple and identifiable linear dynamical model, which is based on the static prediction model of standard therapy. It is shown that the model parameters are intrinsically correlated with physician-set therapy parameters and that the reduction of the number of model parameters to identify leads to inferior data fits but to equivalent or slightly improved prediction capabilities compared to state-of-the-art models: a sign of an appropriate model structure and superior reliability. The validation of the proposed dynamic model is performed using data from the UVa simulator and real clinical data, and potential uses of the proposed model for state estimation and BG control are discussed. PMID:25577673

Bock, Alain; François, Grégory; Gillet, Denis

2015-02-01

220

Comparative toxicity of physiological and biochemical parameters in Euglena gracilis to short-term exposure to potassium sorbate.  

PubMed

Potassium sorbate is the potassium salt of sorbic acid, is a widespread and efficient antioxidant that has multiple functions in plants, traditionally associated with the reactions of photosynthesis; however, it has moderate toxicity to various species including rat, fish, bacteria and human health. The effects of potassium sorbate on the movement and photosynthetic parameters of Euglena gracilis were studied during short-term exposure. Potassium sorbate showed acute toxicity to the green flagellate E. gracilis affecting different physiological parameters used as endpoints in an automatic bioassay such as motility, precision of gravitational orientation (r-value), upward movement and alignment, with mean EC50 values of 2867.2 mg L(-1). The concentrations above 625 mg L(-1) of potassium sorbate induce an inhibition of the photosynthetic efficiency and electron transport rate and, in concentrations more than 2500.0 mg L(-1), the Euglena cells undergo a complete inhibition of photosynthesis even at low light irradiation. PMID:25314908

Engel, Fernanda; Pinto, Luciano Henrique; Del Ciampo, Lineu Fernando; Lorenzi, Luciano; Heyder, Carmen Diamantina Teixeira; Häder, Donat Peter; Erzinger, Gilmar Sidnei

2015-01-01

221

Acute effects of therapeutic ultrasound delivered at varying parameters on the blood flow velocity in a muscular distribution artery.  

PubMed

Therapeutic modalities that alter hemodynamic parameters may have a dramatic impact on the viability of living tissues. The purpose of the current study was to investigate the response of blood flow velocity to various treatment parameters of therapeutic ultrasound. Twenty healthy volunteers attended six randomly selected, 15-minute treatment sessions of the following parameters: Tx-1 = 1.0 MHz at 1.5 W/cm2, Tx-2 = 1.0 MHz at 1.0 W/cm2; Tx-3 = 3.0 MHz at 1.2 W/cm2; Tx-4 = 3.0 MHz at 1.0 W/cm2, Tx-5 = sham; and Tx-6 = control. Ultrasound was applied to a circular area over the triceps surae muscle mass. Blood flow velocity in the popliteal artery was assessed after 5, 10, and 15 minutes of ultrasound and at two posttreatment intervals via a dual frequency, bidirectional ultrasound Doppler. A two-factor analysis of variance (p < or = 0.05) with repeated measures for treatment and time was performed on the data. Groups Tx-1 and Tx-2 showed significant increases in blood flow velocity when compared with the control and all other groups. The sham group showed significant increases in blood flow velocity when compared with the control group. Group Tx-3 and Tx-4 showed no significant change when compared with the sham condition. The results of the current study indicate that 1.0 MHz ultrasound delivered at 1.0 and 1.5 W/cm2 to the triceps surae musculature as described in the present study can increase the blood flow velocity in the popliteal artery. PMID:8902681

Fabrizio, P A; Schmidt, J A; Clemente, F R; Lankiewicz, L A; Levine, Z A

1996-11-01

222

Leaf photosynthesis and respiration of three bioenergy crops in relation to temperature and leaf nitrogen: how conserved are biochemical model parameters among crop species?  

PubMed Central

Given the need for parallel increases in food and energy production from crops in the context of global change, crop simulation models and data sets to feed these models with photosynthesis and respiration parameters are increasingly important. This study provides information on photosynthesis and respiration for three energy crops (sunflower, kenaf, and cynara), reviews relevant information for five other crops (wheat, barley, cotton, tobacco, and grape), and assesses how conserved photosynthesis parameters are among crops. Using large data sets and optimization techniques, the C3 leaf photosynthesis model of Farquhar, von Caemmerer, and Berry (FvCB) and an empirical night respiration model for tested energy crops accounting for effects of temperature and leaf nitrogen were parameterized. Instead of the common approach of using information on net photosynthesis response to CO2 at the stomatal cavity (An–Ci), the model was parameterized by analysing the photosynthesis response to incident light intensity (An–Iinc). Convincing evidence is provided that the maximum Rubisco carboxylation rate or the maximum electron transport rate was very similar whether derived from An–Ci or from An–Iinc data sets. Parameters characterizing Rubisco limitation, electron transport limitation, the degree to which light inhibits leaf respiration, night respiration, and the minimum leaf nitrogen required for photosynthesis were then determined. Model predictions were validated against independent sets. Only a few FvCB parameters were conserved among crop species, thus species-specific FvCB model parameters are needed for crop modelling. Therefore, information from readily available but underexplored An–Iinc data should be re-analysed, thereby expanding the potential of combining classical photosynthetic data and the biochemical model. PMID:22021569

Archontoulis, S. V.; Yin, X.; Vos, J.; Danalatos, N. G.; Struik, P. C.

2012-01-01

223

Association of Renal Biochemical Parameters with Left Ventricular Diastolic Dysfunction in a Community-Based Elderly Population in China: A Cross-Sectional Study  

PubMed Central

Background Relationship of left ventricular diastolic dysfunction (LVDD) with parameters that could provide more information than hemodynamic renal indexes has not been clarified. We aimed to explore the association of comprehensive renal parameters with LVDD in a community-based elderly population. Methods 1,166 community residents (aged ? 65 years, 694 females) participating in the Shanghai Heart Health Study with complete data of renal parameters were investigated. Echocardiography was used to evaluate diastolic function with conventional and tissue Doppler imaging techniques. Serum urea, creatinine, urea-to-creatinine ratio, estimated glomerular filtration rate (eGFR) and urinary albumin-to-creatinine ratio (UACR) were analyzed on their associations with LVDD. Results The prevalence of LVDD increased in proportion to increasing serum urea, urea-to-creatinine ratio and UACR. These three renal parameters were found negatively correlated to peak early (E) to late (A) diastolic velocities ratio (E/A), and positively to left atrial volume index; UACR also positively correlated with E to peak early (E’) diastolic mitral annular velocity ratio (E/E’). Serum urea, urea-to-creatinine ratio and UACR correlated with LVDD in logistic univariate regression analysis, and urea-to-creatinine ratio remained independently correlated to LVDD [Odds ratio (OR) 2.82, 95% confidence interval (CI) 1.34–5.95] after adjustment. Serum urea (OR 1.18, 95%CI 1.03–1.34), creatinine (OR 6.53, 95%CI 1.70­–25.02), eGFR (OR 0.22, 95%CI 0.07–0.65) and UACR (OR 2.15, 95%CI 1.42–3.24) were revealed independent correlates of advanced (moderate and severe) LVDD. Conclusions Biochemical parameters of renal function were closely linked with LVDD. This finding described new cardio-renal relationship in the elderly population. PMID:24533126

Zhou, Jun; Zhang, Hanying; Tang, Bixiao; Fu, Michael; Herlitz, Hans; Cui, Jie; Zhu, Hongmin; Sun, Aijun; Hu, Kai; Ge, Junbo

2014-01-01

224

Biochemical, photosynthetic and productive parameters of Chinese cabbage grown under blue-red LED assembly designed for space agriculture  

NASA Astrophysics Data System (ADS)

Currently light emitting diodes (LEDs) are considered to be most preferable source for space plant growth facilities. We performed a complex study of growth and photosynthesis in Chinese cabbage plants (Brassica chinensis L.) grown with continuous LED lighting based on red (650 nm) and blue (470 nm) LEDs with a red to blue photon ratio of 7:1. Plants grown with high-pressure sodium (HPS) lamps were used as a control. PPF levels used were about 100 ?mol/(m2 s) (PPF 100) and nearly 400 ?mol/(m2 s) (PPF 400). One group of plants was grown with PPF 100 and transferred to PPF 400 at the age of 12 days. Plants were studied at the age of 15 and 28 days (harvest age); some plants were left to naturally end their life cycle. We studied a number of parameters reflecting different stages of photosynthesis: photosynthetic pigment content; chlorophyll fluorescence parameters (photosystem II quantum yield, photochemical and non-photochemical chlorophyll fluorescence quenching); electron transport rate, proton gradient on thylakoid membranes (?pH), and photophosphorylation rate in isolated chloroplasts. We also tested parameters reflecting plant growth and productivity: shoot and root fresh and dry weight, sugar content and ascorbic acid content in shoots. Our results had shown that at PPF 100, plants grown with LEDs did not differ from control plants in shoot fresh weight, but showed substantial differences in photophosphorylation rate and sugar content. Differences observed in plants grown with PPF 100 become more pronounced in plants grown with PPF 400. Most parameters characterizing the plant photosynthetic performance, such as photosynthetic pigment content, electron transport rate, and ?pH did not react strongly to light spectrum. Photophosphorylation rate differed strongly in plants grown with different spectrum and PPF level, but did not always reflect final plant yield. Results of the present work suggest that narrow-band LED lighting caused changes in Chinese cabbage plants on levels of the photosynthetic apparatus and the whole plant, concerning its development and adaptation to a varying PPF level.

Avercheva, Olga; Berkovich, Yuliy A.; Smolyanina, Svetlana; Bassarskaya, Elizaveta; Pogosyan, Sergey; Ptushenko, Vasiliy; Erokhin, Alexei; Zhigalova, Tatiana

2014-06-01

225

Concentration of blood and seminal plasma elements and their relationships with semen parameters in men with spinal cord injury.  

PubMed

The relationship of calcium, magnesium, zinc (Zn) and copper (Cu) concentrations in blood and seminal plasma on sperm parameters of spinal cord-injured (SCI) men and of normal controls was determined. The study included 93 SCI men and 145 neurologically intact men. Colorimetric and flame atomic absorption spectrophotometers were used. Seminal Zc and Cu had a relationship with sperm motility in SCI men. PMID:19143726

Salsabili, N; Mehrsai, A R; Jalaie, S

2009-02-01

226

Hematologic and plasma biochemical reference values in Indian peafowl (Pavo cristatus).  

PubMed

Blood samples were collected from captive, adult, clinically normal Indian peafowl (Pavo cristatus) for hematologic and plasma biochemical analyses. Hematologic parameters investigated were total red blood cell count, hemoglobin, packed cell volume, fibrinogen, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, total white blood cell count, differential white blood cell count, and thrombocyte count. Plasma biochemical parameters investigated were alanine aminotransferase, alkaline phosphatase, amylase, aspartate aminotransferase, bile acids, total bilirubin, blood urea nitrogen, calcium, cholesterol, creatinine, creatine kinase, gamma glutamyltransferase, lactate dehydrogenase, glucose, iron, phosphorus, and uric acid, as well as plasma protein electrophoresis. Results were compared with values from studies done in houbara bustards (Chlamydotis undulata), kori bustards (Ardeotis kori), stone curlews (Burhinus oedicnemus), and taxonomically related species, including ring-necked pheasants (Phasianus colchicus), red-legged partridges (Alectoris rufa), Kashmir native fowl (Kashmirfavorella), and Bangladesh native, Fayoumi, and Assil fowl (Gallus domesticus). PMID:20806654

Samour, Jaime; Naldo, Jesus; Rahman, Habeeb; Sakkir, Mohammed

2010-06-01

227

Physiological and biochemical parameters for identification of QTLs controlling the winter triticale drought tolerance at the seedling stage.  

PubMed

The genetic map of the triticale is created on the basis of double-haploid (DH) lines, derived from F1 hybrids of a cross between the parental line Saka3006 and Modus. In order to localise drought resistance genes, it is necessary to find a phenotype feature which clearly differentiates between parental lines under drought stress conditions. With the future in mind, the aim of the presented studies was to analyse differences in the response to drought stress, between Saka3006 and Modus. Analyses of the water status of leaves, and the activity of the photosynthetic apparatus and protective mechanisms relating to the accumulation of phenolic compounds, were carried out. The studies were completed during the tillering phase. Statistically significant changes, between genotypes experiencing the drought period, were noticed for the osmotic potential, leaf water content, some parameters of chlorophyll fluorescence, and for phenolics and the ferulic acid content. On the basis of the studies, the Saka genotype can be considered drought resistant due to higher leaf water content caused, probably, by smaller hydraulic resistance relative to Modus. The activity of its photosynthetic apparatus during drought was higher than that for the Modus genotype. The high level of phenolic compounds, which can act as photoprotectors and free radical scavengers, was also maintained. All the mentioned parameters can represent the potential phenotype features, which allow the identification of resistance genes on the genetic map of the triticale, which is currently being created. PMID:19059788

Hura, Tomasz; Hura, Katarzyna; Grzesiak, Stanis?aw

2009-03-01

228

Organohalogen contaminants and Blood plasma clinical-chemical parameters in three colonies of North Atlantic Great skua (Stercorarius skua).  

PubMed

The present study compares blood plasma clinical-chemical parameters (BCCPs) in birds from three geographically distinct North Atlantic Great skua (Stercorarius skua) colonies. Birds from these sites bioaccumulate different POP (persistent organic pollutant) concentrations and that enabled us to compare Great skua BCCPs in different exposure scenarios. Persistent organic pollutants (organochlorines: PCB, DDT, chlordanes, HCB, HCH, mirex and brominated flame retardants: PBDEs) and nineteen BCCPs were analysed in 114 adult Great skuas sampled during summer 2009 in North Atlantic colonies at Bjørnøya (n=42), Iceland (n=57) and Shetland (n=15). Specimens from Bjørnøya had the highest blood plasma concentrations of all contaminant groups followed by Iceland and Shetland birds, respectively (ANOVA: p<0.05). Most of the 19 BCCP parameters followed the pattern of colony differences found for contaminants, with Bjørnøya having the highest concentrations. However seven BCCPs, the three liver enzymes ALKP, ALAT and GGT as well as bile acids, cholesterol, sodium and potassium, did not differ between colonies (ANOVA: p>0.05). Therefore correlation analyses of these seven BCCPs vs. POPs were done on the combined colony data while the analyses of the remaining 12 BCCPs were carried out for each colony separately. The analyses of combined colony data showed that the blood plasma concentration of liver enzymes ALAT and GGT increased with increasing concentrations of ?PBDE and ?HCH, HCB and ?CHL, respectively (all Pearson's p<0.05). In Great skuas from Shetland, the important osmotic transport protein albumin increased with increasing concentrations of ?PCB and ?DDT, while total blood plasma protein increased with ?PCB, ?DDT, ?HCH and HCB concentrations (all Pearson's p<0.05). In both Bjørnøya and Iceland skuas, blood plasma pancreatic enzyme amylase decreased with increasing ?HCH concentrations while the erythrocyte waste product total bilirubin in blood plasma increased with increasing ?HCH and ?PBDE concentrations in Iceland Great skuas (all Pearson's p<0.05). In Bjørnøya birds, blood plasma urea from protein metabolism (reflects kidney function) increased with increasing ?PBDE concentrations (Pearson's p<0.05). Furthermore, a redundancy analysis showed that 10.6% of the variations in BCCPs could be explained by the variations in POP concentrations. Based on these results we suggest that liver and renal functions could be negatively affected by different POP compounds. It is, however, uncertain if the colony BCCP differences and their relationship to POP concentrations reflect health effects that could have an overall impact on the populations via reduced survival and reproduction parameters. PMID:23537726

Sonne, Christian; Rigét, Frank F; Leat, Eliza H K; Bourgeon, Sophie; Borgå, Katrine; Strøm, Hallvard; Hanssen, Sveinn A; Gabrielsen, Geir W; Petersen, Aevar; Olafsdottir, Kristin; Magnusdottir, Ellen; Bustnes, Jan O; Furness, Robert W; Kjelgaard-Hansen, Mads

2013-06-01

229

Integrated assessment of water quality of the Costa da Morte (Galicia, NW Spain) by means of mussel chemical, biochemical and physiological parameters.  

PubMed

The aim of this study was to assess environmental quality at some of the sites most severely affected by the Prestige oil spill off 2 years after the spillage (April and November 2004). For this purpose analyses of polycyclic aromatic hydrocarbons (PAH) and several biochemical (antioxidant enzymes catalase, glutathione reductase, glutathione peroxidase, superoxide dismutase and DT-diaphorase and lipid peroxidation) and physiological [scope for growth (SFG)] biomarkers were determined on wild mussel populations (Mytilus galloprovincialis) collected at four points along the Costa da Morte and compared with those of a reference site not affected by the oil spill. Results showed that PAH contents had markedly decreased 17 months after the accident, although they were higher in April than in November, when they showed values similar to background levels reported for this area. Nevertheless, the predominance of chrysene on PAH profiles, similarly to findings obtained immediately after the spill, indicated the Prestige as their main source. In spite of the low PAH levels recorded, antioxidant activity levels (explained through the integrated antioxidant response-IAR) were higher in the Costa da Morte than at the reference site either in April and November. In April IAR seems to be related to PAH levels found 3 months after the accident (February 2003), suggesting the persistence in the environment of oxidative stress-producing components from the spill. However, evidence of oxidative stress was not reflected at physiological level by scope for growth, with only very slight differences being observed between values from the reference site and those from Costa da Morte sites. In conclusion, although 2 years after the spill PAHs bioaccumulated by mussels from the Costa da Morte had decreased to background levels, biochemical parameters showed signals of oxidative stress in mussels from this area. However, SFG reflected a good health status for the mussel populations studied and did not reveal evidence of physiological disturbance either 17 or 24 months after the Prestige spill. PMID:20033486

Fernández, Beatriz; Albentosa, Marina; Viñas, Lucía; Franco, Angeles; González, Juan J; Campillo, Juan A

2010-04-01

230

EFFECTS OF HEMORRHAGIC STRESS ON SEVERAL BLOOD PARAMETERS IN ADULT RAINBOW TROUT ('SALMO GAIRDNERI')  

EPA Science Inventory

Blood was removed from ten adult rainbow trout (Salmo gairdneri) on a sequential (7 days) weekly (4 weeks) and monthly (1 month) schedule and analyzed for hematocrit, plasma protein, acid phosphatase (AP), lactic dehydrogenase (LDH) and creatine phosphokinase (CPK). Of the parame...

231

Comparison of Total Antioxidant Capacity Oxidative Stress and Blood Lipoprotein Parameters in Volleyball Players and Sedentary  

ERIC Educational Resources Information Center

This study aims to measure, then compare sedentary blood lipoproteins, oxidant- antioxidant state and oxidative stress index in volleyball players. The experimental group of the research consists of regularly practising 20 boys between the ages of 12 and 17, and the control group comprises 32 children practising no particular sports branch, 12 of…

Gokhan, Ismail

2013-01-01

232

Seasonal baseline levels of physiological and biochemical parameters in polar cod (Boreogadus saida): Implications for environmental monitoring.  

PubMed

Seasonality of biomarker baseline levels were studied in polar cod (Boreogadus saida), caught in Kongsfjorden, Svalbard, in April, July, September and December, 2006-2007. Physiological parameters (condition factor, gonado- and hepato-somatic indexes, energy reserves, potential metabolic activity and antifreeze activity) in polar cod were used to interpret the seasonality of potential biomarkers. The highest levels of ethoxyresorufin-O-deethylase (EROD) activity occurred concomitantly with the highest potential metabolic activity in July due to e.g. intense feeding. During pre-spawning, EROD showed significant inhibition and gender differences. Hence, its potential use in environmental monitoring should imply gender differentiation at least during this period. Glutathione S-transferase and catalase activities were stable from April to September, but changed in December suggesting a link to low biological activity. Knowledge of the biomarker baseline levels and their seasonal trends in polar cod is essential for a trustworthy interpretation of forthcoming toxicity data and environmental monitoring in the Arctic. PMID:20385393

Nahrgang, Jasmine; Camus, Lionel; Broms, Fredrik; Christiansen, Jørgen S; Hop, Haakon

2010-08-01

233

Effects of Different Levels of Coriander (Coriandrum sativum) Seed Powder and Extract on Serum Biochemical Parameters, Microbiota, and Immunity in Broiler Chicks  

PubMed Central

The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28?d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

Hosseinzadeh, Hesam; Alaw Qotbi, Ali Ahmad; Seidavi, Alireza; Norris, David; Brown, David

2014-01-01

234

Assessment of clinical biochemical parameters in Roma minority residing in eastern Slovakia compared with the majority population.  

PubMed

Roma constitute the largest ethnic minority in Europe and the second largest minority in Slovakia. Their health problems originate mainly from their low socioeconomic status, certain cultural aspects and their health-threatening lifestyle as well as the psycho-social burden arising from poverty and frequent migration. Evaluation of glucose, albumin, triacylglycerol (TAG) and low density lipoprotein cholesterol (LDL-C) concentrations did not reveal any clue about the presumed deteriorated health of the Roma population. Higher proportions of subjects with elevated serum total cholesterol were found in Roma women as compared to both control groups of women (p = 0.027, p = 0.006) and in Roma men as compared to the male control group living in standard conditions. Only the low level of HDL-cholesterol gives a glimpse of their deteriorated health. Significantly lower levels of serum HDL-C were reported in Roma men and women compared to the respondents in both control groups with a p value of p < 0.001. Comparing the ratio of LDL-C/HDL-C yielded significant differences between the number of physiological values in Roma men and men from the control group 1 (p = 0.022) in favour of the control group. When comparing the number of people with physiological values of cholesterols and with worsening TAG parameters at the same time, the increased risk of Roma men compared with men from the control group 1 became evident, with a level of significance of p = 0.023. Evaluation of urine samples pointed to significantly higher concentrations of urinary protein in Roma women compared with women in the control group 1 (p = 0.012). PMID:24847608

Hubková, Beáta; Maslanková, Jana; Stupák, Marek; Guzy, Juraj; Kovácová, Anna; Pella, Daniel; Jarcuska, Peter; Mareková, Mária

2014-03-01

235

Evaluation of blood pressure control using a new arterial stiffness parameter, cardio-ankle vascular index (CAVI).  

PubMed

Arterial stiffness has been known to be a surrogate marker of arteriosclerosis, and also of vascular function. Pulse wave velocity (PWV) had been the most popular index and was known to be a predictor of cardiovascular events. But, it depends on blood pressure at measuring time. To overcome this problem, cardio-ankle vascular index (CAVI) is developed. CAVI is derived from stiffness parameter ? by Hayashi, and the equation of Bramwell-Hill, and is independent from blood pressure at a measuring time. Then, CAVI might reflect the proper change of arterial wall by antihypertensive agents. CAVI shows high value with aging and in many arteriosclerotic diseases and is also high in persons with main coronary risk factors. Furthermore, CAVI is decreased by an administration of ?1 blocker, doxazosin for 2-4 hours, Those results suggested that CAVI reflected the arterial stiffness composed of organic components and of smooth muscle cell contracture. Angiotensin II receptor blocker, olmesartan decreased CAVI much more than that of calcium channel antagonist, amlodipine, even though the rates of decreased blood pressure were almost same. CAVI might differentiate the blood pressure-lowering agents from the point of the effects on proper arterial stiffness. This paper reviewed the principle and rationale of CAVI, and the possibilities of clinical applications, especially in the studies of hypertension. PMID:23807874

Shirai, Kohji; Utino, Junji; Saiki, Atsuhito; Endo, Kei; Ohira, Masahiro; Nagayama, Daiji; Tatsuno, Ichiro; Shimizu, Kazuhiro; Takahashi, Mao; Takahara, Akira

2013-02-01

236

An experimental soft-release of oil-spill rehabilitated American coots ( Fulica americana): II. Effects on health and blood parameters  

Microsoft Academic Search

The Unocal–Metrolink oil spill of 21 February 1995 resulted in approximately 7800 barrels of San Joaquin crude oil being deposited into the San Gabriel River in Huntington Beach, CA, USA. In order to determine long-term pathological effects of oil exposure and rehabilitation, hematological and serum biochemical parameters for both rehabilitated (RHB) American coots (Fulica americana) and reference (REF) coots were

S. H. Newman; D. W. Anderson; M. H. Ziccardi; J. G. Trupkiewicz; F. S. Tseng; M. M. Christopher; J. G. Zinkl

2000-01-01

237

Temperature correction of arterial blood-gas parameters: A comparative review of methodology.  

PubMed

The need for accurate clinical diagnosis and appropriate intervention requires that a modern blood-gas laboratory have the means to correct for significant discrepancies between patient temperature and the temperature at which in vitro blood samples are analyzed. Recent advances in mini- and microcomputer technology permit application of any or all of the correction formulas above at modest cost and minimal inconvenience (See the Appendix). An expanded program for a TI-59 desk-top calculator and P-100C printer which gives labeled hard-copy readout of temperature-corrected pH, PCO2, PO2, and hemoglobin saturation values, as well as bicarbonate concentration and in vivo base excess is in daily clinical use in our operating room and is available from the authors upon request. PMID:6791530

Andritsch, R F; Muravchick, S; Gold, M I

1981-09-01

238

The Effect of Age on Hematological and Serum Biochemical Values on Juvenile Ring-Necked Pheasants (Phasianus colchicus)  

Microsoft Academic Search

1 5 Abstract: The influences of age on hematological and serum biochemical parameters were investigated in juvenile ring-necked pheasant. Statistical comparisons were made for differences in values among different ages. A variety of blood parameters showed significantly age related differences. Juvenile ring- necked pheasants of all ages had lymphocytes as the major circulating leukocyte. RBC values for 60 day-old pheasants

Elizabeth Moreira dos Santos Schmidt; Antonio Carlos Paulillo; Rosangela Locatelli Dittrich; Elizabeth Santin; Paula Cristina Linder da Silva; Olair Beltrame; Edson Goncalves de Oliveira

2007-01-01

239

Role of sudarshan kriya and pranayam on lipid profile and blood cell parameters during exam stress: A randomized controlled trial  

PubMed Central

Background: Yoga is a science practiced in India over thousands of years. It produces constituent physiological changes and has sound scientific basis. Aim: Since exam stress modifies lipid profile and hematological parameters, we conducted an investigation on the effect of sudarshan kriya (SK and P) program on these parameters. Materials and Methods: Blood samples of 43 engineering students were collected at four intervals namely baseline (BL), exam stress (ES), three and six weeks practice of SK and P during exam stress. Lipid profile and hematological parameters were measured at all four intervals. Results: ES elevated total cholesterol (TC), triglycerides (TGL) and very low density lipoprotein (VLDL) levels. Hematological parameters affected by ES included neutrophil, lymphocytes, platelet count, packed cell volume (PCV) and mean cell volume (MCV). Three and six weeks practice of SK and P reduced the elevated lipid profile, hematological parameters and improved lymphocyte levels. Conclusion: Our study indicates that SK and P practice has the potential to overcome ES by improving lipid profile and hematological parameters. PMID:22346062

Subramanian, Swapna; Elango, Tamilselvi; Malligarjunan, Hemamalini; Kochupillai, Vinod; Dayalan, Haripriya

2012-01-01

240

Hypolipidemic and antioxidative effects of curcumin on blood parameters, humoral immunity, and jejunum histology in Hy-line hens  

PubMed Central

Objective: Turmeric (Curcuma Longa Linn) is a medicinal plant that contains curcumin. There is a growing interest in using curcumin powder (CP) as feed additives for antioxidative and antimicrobial properties to improve human health. This study was conducted to determine the appropriate levels of CP on blood parameters, immunity, and histology of jejunum in hens. Materials and Methods: A total of 200, 58-wk-old Hy-line hens were randomly distributed into 4 treatments (0%, 0.5%, 1.5%, and 2.5% CP or 0, 5, 15, and 25 g/kg feed, respectively) with 5 replicates (10 birds each) for 8 weeks using the completely randomized design. Blood samples were taken from 2 birds per replicate at weeks 61 and 65 to evaluate blood parameters. On weeks 63 and 65, two birds from each replicate received 0.5 ml SRBC (25%) injection in breast muscle and 7 days later, blood samples were collected to evaluate total Ig, IgG, and IgM titers in serum. Two hens were sacrificed at week 65 for the histological study of jejunum. Results: Curcumin reduced triglycerides at 1.5% and 2.5% and cholesterol and LDL at 2.5% (p<0.05). Improvement of total Ig and IgG titers after the 1st and 2nd injections were observed. Muscle thickness in jejunum increased (p<0.05) at 1.5% curcumin and the length and surface area of villus were enhanced as well. Conclusion: Our results demonstrate that curcumin can be used as an antioxidant at 1.5% and antilipidemic agent at 2.5% in diet. PMID:25050272

Arshami, Javad; Pilevar, Mohammad; Aami Azghadi, Mohammad; Raji, Ahmad Reza

2013-01-01

241

Effects of oral L-carnitine, L-lysine administration and exercise on body composition and histological and biochemical parameters in pigeons.  

PubMed

The purpose of this study was to examine whether L-carnitine and its precursor L-lysine could have any beneficial effect in racing pigeons, and if so, whether this effect is influenced by the extent of exercise (short-distance flight: 135 km vs. long-distance flight: 580 km). Birds were divided into seven groups of animals. Group 1: negative control, no flight, no treatment, Group 2: positive control, placebo treatment before the short-distance flight, Group 3: 200 mg/day L-carnitine treatment before the short-distance flight, Group 4: 400 mg/day L-lysine treatment before the short-distance flight, Group 5: positive control, placebo treatment before the long-distance flight, Group 6: 200 mg/day L-carnitine treatment before the long-distance flight, Group 7: 400 mg/day L-lysine treatment before the long-distance flight. L-carnitine, L-lysine and distilled water (placebo) were orally administered (tube feeding) for 7 days before flight. Just after returning home, blood samples were collected and analyzed for glucose, fructosamine, cholesterol, triglycerides and thiobarbituric acid reactive substances. Pigeons were euthanized using carbon dioxide as an inhalation agent, and the whole body was subjected to proximate analysis. The status at arrival was referred to as a basis for comparison. Sex did not affect the measured parameters. As a result of the L-carnitine and L-lysine administrations, the body fat mobilization was higher during the 580 km flight, whereas no changes were noted during the 135 km flight. The main changes in the measured blood parameters were caused by the extent of exercise. This experiment considered the extent of exercise as a factor potentially modulating L-carnitine supplementation effects. In conclusion, flight distance affected several parameters but the supplements of L-carnitine and L-lysine were not effective in the tested situations. PMID:18477325

Hullár, I; Fekete, S G; Mézes, M; Glávits, R; Gáspárdy, A; Fébel, H

2008-06-01

242

Allometric Growth of Testes in Relation to Age, Body Weight and Selected Blood Parameters in Male Japanese Quail (Coturnix japonica).  

PubMed

The Japanese quail is a very valuable animal model for research in a variety of biological disciplines. The purpose of this study was to characterize and interrelate age-dependent testicular parameters with various blood constituents: blood glucose, plasma proteins and packed cell volume that are developing concurrently in the growing bird. Another objective of the study was to identify selective physioanatomical markers for predicting the testicular growth and the onset of sexual maturity. Male Japanese quail hatchlings were raised in temperature controlled brooders for up to 3 weeks of age under a constant light and then shifted to hanging cages in an air conditioned room set at ~73° F under a 14L: 10D lighting system and ad libitum access to feed and water. Starting d8, a group of 8-10 birds of uniform size and weight were selected randomly at 4-day intervals up to d52 of age for the project. The birds were weighed and blood sampled using the brachial vein and Blood Glucose (BGL), Total Plasma Proteins (PP) and Packed Cell Volume (PCV) levels were measured prior to euthanization. The testes were removed and measured for weight, length, width and Volume (VOL). All the testicular measurements were then correlated with age and body weight. The left testes were larger than the right testes and their differences were evident at d36 of age. Testicular measurements also reflected two distinct growth surges at d28, d32 and d36 of age. Combined Testes Weight (CTW) and Combined Testes Volume (CTV) revealed a strong positive correlation with PCV and PP and a negative correlation with Blood Glucose Level (BGL). Accordingly, these measurements could serve as reliable markers of growth rate and sexual maturation in male Japanese quail. PMID:25243007

Vatsalya, Vatsalya; Arora, Kashmiri L

2012-01-01

243

Artificial blood  

PubMed Central

Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone. PMID:19742251

Sarkar, Suman

2008-01-01

244

Effects of chromium propionate on egg production, egg quality, plasma biochemical parameters, and egg chromium deposition in late-phase laying hens.  

PubMed

This study was conducted to investigate the effects of chromium propionate on egg production, egg quality, plasma biochemical parameters and egg chromium deposition in late-phase laying hens. Four hundred thirty-two 60-weeks old laying hens were divided into four groups of 108 birds per group according to egg production. The dietary treatments consisted of the basal diet adding with 0, 200, 400, and 600 ?g/kg chromium as chromium propionate. All laying hens were given feed and water ad libitum for 8 weeks. The addition of 400 ?g/kg Cr as chromium propionate increased egg production (P??0.05). These data indicated that feeding of late-phase laying hens with chromium propionate could improve egg production, increase eggshell thickness, but do not result in abnormal levels of chromium deposition in eggs. PMID:24338491

Ma, Wenqiang; Gu, Ying; Lu, Jingyu; Yuan, Lvfeng; Zhao, Ruqian

2014-02-01

245

The Effects of UV-B Radiation Intensity on Biochemical Parameters and Active Ingredients in Flowers of Qi Chrysanthemum and Huai Chrysanthemum.  

PubMed

The article studied UV-B effects on biochemical parameters and active ingredients in flowers of Qi chrysanthemum and Huai chrysanthemum during the bud stage. The experiment included four UV-B radiation levels (CK, ambient UV-B; T1, T2 and T3 indicated a 5%, 10% and 15% increase in ambient UV-BBE , respectively) to determine the optimal UV-B radiation intensity in regulating active ingredients level in flowers of two chrysanthemum varieties. Flower dry weight of two cultivars was not affected by UV-B radiation under experimental conditions reported here. UV-B treatments significantly increased the rate of superoxide radical production, hydrogen peroxide (H2 O2 ) (except for T1) and malondialdehyde concentration in flowers of Huai chrysanthemum and H2 O2 concentration in flowers of Qi chrysanthemum. T2 and T3 treatments induced a significant increase in phenylalanine ammonia lyase enzyme (PAL) activity, anthocyanins, proline, ascorbic acid, chlorogenic acid and flavone content in flowers of two chrysanthemum varieties, and there were no significant differences in PAL activity, ascorbic acid, flavone and chlorogenic acid content between the two treatments. These results indicated that appropriate UV-B radiation intensity did not result in the decrease in flower yield, and could regulate PAL activity and increase active ingredients content in flowers of two chrysanthemum varieties. PMID:25112378

Yao, Xiao-Qin; Chu, Jian-Zhou; He, Xue-Li; Si, Chao

2014-11-01

246

A Multiple Parameters Biodosimetry Tool with Various Blood Cell Counts - the Hemodose Approach  

NASA Technical Reports Server (NTRS)

There continue to be important concerns about the possibility of the occurrence of acute radiation syndromes following nuclear and radiological terrorism or accidents that may result in mass casualties in densely populated areas. To guide medical personnel in their clinical decisions for effective medical management and treatment of the exposed individuals, biological markers are usually applied to examine radiation induced biological changes to assess the severity of radiation injury to sensitive organ systems. Among these the peripheral blood cell counts are widely used to assess the extent of radiation induced bone marrow injury. This is due to the fact that the hematopoietic system is the most vulnerable part of the human body to radiation damage. Particularly, the lymphocyte, granulocyte, and platelet cells are the most radiosensitive of the blood elements, and monitoring their changes after exposure is regarded as a practical and recommended laboratory test to estimate radiation dose and injury. Based upon years of physiological and pathophysiological investigation of mammalian hematopoietic systems, and rigorous coarse-grained bio-mathematical modeling and validation on species from mouse, to dog, monkey, and human, we have developed a set of software tools Hemodose, which can use single or serial granulocyte, lymphocyte, leukocyte, or platelet counts after exposure to estimate absorbed doses of adult victims very rapidly and accurately. Some patient data from historical accidents are utilized as examples to demonstrate the capabilities of these tools as a rapid point-of-care diagnostic or centralized high-throughput assay system in a large-scale radiological disaster scenario. Most significant to the improvement of national and local preparedness of a potential nuclear/radiological disaster, this HemoDose approach establishes robust correlations between the absorbed doses and victim's various types of blood cell counts not only in the early time window (1 or 2 days), but also in the very late phase (up to 4 weeks) after exposure.

Hu, Shaowen

2014-01-01

247

Relationships between repeated sprint ability, mechanical parameters and blood metabolites in professional soccer players.  

PubMed

This study analysed the acute metabolic and mechanical responses to a specific Repeated Sprint Ability (RSA) test. Eighteen male professional soccer players from a team of the First Division of Spanish National League participated. A 12x30m RSA test with 30 s recovery together with countermovement jump test (CMJ) pre a post RSA test was performed. Mechanical responses (i.e. height performance in CMJ and speed loss) and metabolic responses (i.e. blood lactate, and ammonia concentrations) were measured pre-exercise and post-exercise. A related samples t-test was used to analyse CMJ height pre-post changes as well as to compare pre- and post-exercise lactate and ammonia levels. CMJ height loss pre-post session (8%) was significant, and fatigue, measured as CMJ height loss, was strongly correlated to lactate (r = 0.97; p<0.001) and ammonia (r = 0.92; p<0.001) for all players. The relationships between the variables studied were determined by calculating the Pearson correlation coefficients. The metabolic stress developed during the effort can be estimated by controlling CMJ because of the high correlation between CMJ and blood lactate and ammonia concentrations. The high correlations found between mechanical (speed and CMJ height losses) and metabolic (lactate and ammonia) measures of fatigue highlight the utility and validity of using CMJ to monitor training load and quantify objectively neuromuscular fatigue during RSA. PMID:25463691

Morcillo, Jose A; Cuadrado, Victor; Jiménez-Reyes, Pedro; Ortega-Becerra, Manuel; Lozano, Emilio; Párraga, Juan

2014-12-01

248

BIOCHEMICAL CHANGES IN HUMANS UPON EXPOSURE TO SULFURIC ACID AEROSOL AND EXERCISE  

EPA Science Inventory

A total of 18 human subjects were exposed to ambient air for four hours on the first day of exposure and to four hours of 100 micrograms/cu. m. (0.033 micromole) sulfuric acid aerosol exposed to four hours of ambient air on both exposure days. Six biochemical blood parameters wer...

249

Normal Hematologic and Serum Biochemical Values of Cotton-Top Tamarins (Saguinus oedipus)  

PubMed Central

We obtained whole-blood hematologic and serum biochemical values from 38 captive-bred cotton-top tamarins (Saguinus oedipus). Data were analyzed to determine the effect of sex on blood parameters. Significant differences between either the means or medians of male and female tamarins were found for creatinine, hematocrit, hemoglobin, RBC count, and PCV. These results establish baseline hematologic and serum biochemical values and provide a useful resource not previously available in the peer-reviewed literature for the clinical care of cotton-top tamarins, a critically endangered New World primate, in a captive setting. PMID:22776113

Shukan, Evan T; Boe, Carla Y; Hasenfus, Aimee V; Pieper, Bridget A; Snowdon, Charles T

2012-01-01

250

Evaluation of adrenal function, growth, carcass characteristics, blood metabolites, hematological and immune parameters in Angus, Brahman, Bonsmara X Angus and Bonsmara beef steers  

E-print Network

Adrenal function, blood metabolites, hematological parameters, growth, and carcass characteristics were compared in tropically-adapted (Brahman,) intermediate (Bonsmara and Bonsmara X Angus crossbred,) and temperate (Angus; n=10 each) beef steers...

Jacobs Hollenbeck, Regina

2006-10-30

251

Towards noninvasive method for the detection of pathological tissue variations by mapping different blood parameters  

NASA Astrophysics Data System (ADS)

This paper describes the development of an early detection method for probing pathological tissue variations. The method could be used for classifying various tissue alteration namely tumors tissue or skin disorders. The used approach is based on light scattering and absorption spectroscopy. Spectral content of the scattered light provides diagnostic information about the tissue contents. The importance of this method is using a safe light that has less power than the used in the imaging methods that will enable the frequent examination of tissue, while the exiting modalities have drawbacks like ionization, high cost, time-consuming, and agents' usage. A modality for mapping the oxygen saturation distribution in tissues noninvasively is new in this area of research, since this study focuses on the oxygen molecule in the tissue which supposed to be homogenously distributed through the tissues. Cancers may cause greater vascularization and greater oxygen consumption than in normal tissue. Therefore, oxygen existence and homogeneity will be alternated depending on the tissue state. In the proposed system, the signal was extracted after illuminating the tissue by light emitting diodes (LED's) that emits light in two wavelengths, red (660 nm) and infrared (880 nm). The absorption in these wavelengths is mainly due to oxyhemoglobin (HbO2) and deoxyhemoglobin (Hb) while other blood and tissue contents nearly have low effect on the signal. The backscattered signal which is received by a photodiodes array (128 PDs) was measured and processed using LabVIEW. Photoplethysmogram (PPG) signals have been measured at different locations. These signals will be used to differentiate between the normal and the pathological tissues. Variations in hemoglobin concentration and blood perfusion will also be used as an important indication feature for this purpose.

Abdallah, Omar; Qananwah, Qasem; Abo Alam, Kawther; Bolz, Armin

2010-04-01

252

Influence of different amounts and sources of selenium supplementation on performance, some blood parameters, and nutrient digestibility in lambs.  

PubMed

Two trials were conducted in a 2?×?2?+?1 factorial arrangement based on a completely randomized design to evaluate the effects of different sources of selenium (Se) on performance, blood metabolites, and nutrient digestibility in male lambs on a barley-based diet. The first trial lasted for 70 days and consisted of 30 lambs (35.6?±?2.6 kg mean body weight, about 4-5 months of age) which were randomly allotted to five treatments including: (1) basal diet (containing 0.06 mg Se/kg DM; control) without supplementary Se, (2) basal diet?+?0.20 mg/kg Se as sodium selenite (SeS 0.20), (3) basal diet?+?0.40 mg/kg Se as sodium selenite (SeS 0.40), (4) basal diet?+?0.20 mg/kg Se as selenium yeast (SeY 0.20), and (5) basal diet?+?0.40 mg/kg Se as selenium yeast (SeY 0.40). For the second trial, four lambs from each group of experiment 1 were randomly allocated to individual metabolic cages for 14 days to measure the effects of dietary Se on nutrient digestibility. The results revealed that there were no significant differences for average daily gain, average daily feed intake, feed/gain ratio, hematological parameters (packed cell volume, red blood cell, white blood cell, and hemoglobin values), serum total protein, albumin, globulin, aspartate amino transferase, alkaline phosphatase, and creatine phosphokinase due to supplementation of different amounts and sources of Se in lambs. Dietary Se supplementation significantly improved (P?blood. Furthermore, at the end of the trial, serum tri-iodothyronine (T3) amount also increased (P?blood hematology, but increased blood glutathione peroxidase activity and serum T3 amount and decreased serum T4 amount as compared to non-supplemented control lambs. Furthermore, Se yeast improved nutrient digestibility in lambs. PMID:23677850

Alimohamady, Reza; Aliarabi, Hassan; Bahari, Aliasghar; Dezfoulian, Amir Hossein

2013-07-01

253

Blind Deconvolution for Distributed Parameter Systems with Unbounded Input and Output and Determining Blood Alcohol Concentration from Transdermal Biosensor Data.  

PubMed

We develop a blind deconvolution scheme for input-output systems described by distributed parameter systems with boundary input and output. An abstract functional analytic theory based on results for the linear quadratic control of infinite dimensional systems with unbounded input and output operators is presented. The blind deconvolution problem is then reformulated as a series of constrained linear and nonlinear optimization problems involving infinite dimensional dynamical systems. A finite dimensional approximation and convergence theory is developed. The theory is applied to the problem of estimating blood or breath alcohol concentration (respectively, BAC or BrAC) from biosensor-measured transdermal alcohol concentration (TAC) in the field. A distributed parameter model with boundary input and output is proposed for the transdermal transport of ethanol from the blood through the skin to the sensor. The problem of estimating BAC or BrAC from the TAC data is formulated as a blind deconvolution problem. A scheme to identify distinct drinking episodes in TAC data based on a Hodrick Prescott filter is discussed. Numerical results involving actual patient data are presented. PMID:24707065

Rosen, I G; Luczak, Susan E; Weiss, Jordan

2014-03-15

254

Neonatal Candida meningitis: significance of cerebrospinal fluid parameters and blood cultures  

Microsoft Academic Search

Objective:The purpose of this study was to examine the frequency of normal cerebrospinal fluid (CSF) parameters in Candida meningitis and the proportion of candidemia associated with Candida meningitis.Study design:We evaluated the initial lumbar puncture results from infants discharged from 150 Neonatal Intensive Care Units between 1997 and 2004. Candida meningitis was diagnosed by a positive CSF culture or positive Gram

M Cohen-Wolkowiez; P B Smith; B Mangum; W J Steinbach; B D Alexander; C M Cotten; R H Clark; T J Walsh; D K Benjamin

2007-01-01

255

Parasitological and biochemical parameters in Schistosoma mansoni-infected mice treated with methanol extract from the plants Chenopodium ambrosioides, Conyza dioscorides and Sesbania sesban.  

PubMed

This study aims to detect the antischistosomal properties of the plants' Chenopodium ambrosioides, Conyza dioscorides and Sesbania sesban methanol extract against Schistosoma mansoni in infected mice, including determination of total protein and albumin levels and the activities of alanine and aspartate transaminases (AlT, AsT) and acid and alkaline phosphatases (AcP and AkP) enzymes in the serum of infected treated mice. Male Swiss albino mice were infected with S. mansoni and orally treated with methanol extract of the plants C. ambrosioides (1250 mg/kg/day), C. dioscorides and S. sesban (1000 mg/kg/day from each) for 2 consecutive days 7 weeks post infection (PI). In addition, treatment of mice with the tested dose of each plant extract was successively done (i.e. the 1st extract followed by the 2nd and 3rd one with an hour interval). Parasitological and biochemical parameters were assessed. Nine weeks PI, the reduction rates of worm load/mouse treated with either C. dioscorides (1000 mg/kg), C. ambrosioides (1250 mg/kg) or S. sesban (1000 mg/kg) were 40.9%, 53.7% and 54.4%, respectively. Successive treatment raised the reduction rates of worm load/mouse to 66.3% and the ova/g tissue in liver to 76.9%. Moreover, serum total protein and albumin levels and activities of AlT, Ast, AcP and AkP enzymes of infected treated mice were improved in comparison with those of infected untreated ones. It is concluded that administration of C. dioscorides, C. ambrosioides and S. sesban methanol extract to infected mice exhibited a moderate antischistosomal effect. Successive treatment improved the antischistosomal properties of these plant species, hence ameliorated the liver functions of treated mice that may suggest degenerations of liver granulomas and regenerative changes. PMID:21740980

Kamel, E G; El-Emam, M A; Mahmoud, S S M; Fouda, F M; Bayaumy, F E

2011-12-01

256

Dopamine-?-Hydroxylase Activity and Levels of Its Cofactors and Other Biochemical Parameters in the Serum of Arsenicosis Patients of Bangladesh  

PubMed Central

Dopamine-?-hydroxylase (DBH) is a neurotransmitter (catecholamine)-mediating enzyme, which catalyzes the formation of norepinephrine from dopamine. The levels of DBH activity, its coenzyme (ascorbic acid) and cofactor (Cu++) and other biochemical parameters were measured in the serum of 32 arsenicosis patients of Bangladesh at three different age groups, namely, group 1 (10–18 years, 9 patients), group 2 (19–40 years, 14 patients) and group 3 (41–70 years, 9 patients) of the locality of Stadium Para of Meherpur district of Bangladesh. The values were compared with the same number of age-matched normal healthy individuals of the respective group. DBH activity was markedly decreased in the patients of group 1 as compared to that of the normal healthy people. The activities of DBH were decreased to lesser extents for the other two age groups. The total protein contents in the serum of arsenicosis patients were also significantly low as compared to that in the age-matched control groups. The levels of ascorbic acid and copper were found to be decreased in the serum of arsenicosis patients. The serum glucose levels were elevated in arsenicosis patients, as compared to that of the respective healthy controls. Other parameters, such as zinc and vitamin A levels were also decreased in the serum of arsenicosis patients. It was evident from the results of drinking of the arsenic contaminated water of shallow tube wells that the levels of DBH activity decreased significantly as compared to the control healthy persons. The levels of proteins, ascorbic acid, copper, zinc and vitamin A were decreased in the serum of people drinking the arsenic contaminated tube wells water as compared to that in the control healthy people with the exception that the levels of glucose were elevated in the serum of these patients. The pathophysiological significance of the results could be correlated with the decreased in proteins and that in DBH activities as DBH deficiency is characterized by lack of sympathetic noradrenergic function.The general physiologic findings of autonomic function indicate that complete DBH deficiency include minimal or absent plasma norepinephrine and epinephrine. PMID:24711750

Rahman, M. Khalilur; Choudhary, M. Iqbal; Arif, M.; Morshed, M. Monzur

2014-01-01

257

Various Blood Glucose Parameters that Indicate Hyperglycemia after Intravenous Thrombolysis in Acute Ischemic Stroke Could Predict Worse Outcome  

PubMed Central

Background Hyperglycemia is common after stroke, and it is well known to worsen its outcome. However, it is important to consider that blood glucose (BG) levels can undergo dynamic changes during the acute stage of ischemic stroke. We sought to investigate the clinical significance of various glucose parameters within first 24 hours in acute ischemic stroke (AIS). The study focused on hyperacute stage patients who underwent IVT and investigated which parameters of glucose demonstrated to be helpful for predicting outcome. Methods This was a retrospective study of consecutive patients with AIS at a single stroke center. Patients were consecutively enrolled if they were treated with IV-tPA within 3 hours of symptom onset. BG was measured immediately upon arrival in ER, after IVT and every 6–8 hours during the first 24 hours after IVT. The various parameters of BG were the following: BG before IVT, BG after IVT, mean BG (mBG), maximal BG (max BG), standard deviation of BG (sdBG), and standard deviation of mean BG (sdmBG). Results 207 patients (127 men and 80 women) were included in this study. Seventy seven of 207 patients had favorable outcomes at 3 months. High BG after IVT, mBG and max BG were independently associated with mRS>2 at 3 months (adjusted by age, NIHSS, and atrial fibrillation). Several parameters of BG were also independently associated with early mortality within 3 months (BG after IVT, mBG, and max BG). BG after IVT and mBG over 180 mg/dL were independently associated with early mortality within 3 months. Conclusion Serial measurements of BG might be a better predictor of clinical outcome in patients with AIS treated with IVT than single BG measurements before IVT. Therefore, these results suggest that variable parameters of BG could be important for the prediction of clinical outcome in AIS treated with IVT. PMID:24747428

Yoo, Deok-Sang; Chang, Jane; Kim, Joon-Tae; Choi, Min-Ji; Choi, Jina; Choi, Kang-Ho; Park, Man-Seok; Cho, Ki-Hyun

2014-01-01

258

Melatonin treatment improves blood pressure, lipid profile, and parameters of oxidative stress in patients with metabolic syndrome.  

PubMed

Experimental studies have proven that melatonin has many beneficial pleiotropic actions. The aim of this study was to assess melatonin efficacy in patients with metabolic syndrome (MS). The study included 33 healthy volunteers (who were not treated with melatonin) and 30 patients with MS, who did not respond to 3-month lifestyle modification. Patients with MS were treated with melatonin (5 mg/day, 2 hr before bedtime) for 2 months. The following parameters were studied: systolic and diastolic blood pressure (SBP, DBP), levels of glucose, serum lipids, C-reactive protein, fibrinogen, activities of antioxidative enzymes: catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), thiobarbituric acid reactive substrates (TBARS). After 2-month therapy in comparison with baseline, the following significant changes were measured: systolic blood pressure (132.8±9.8 versus 120.5±11.0 mmHg, P<0.001), DBP (81.7±8.8 versus 75±7.4 mmHg, P<0.01), low-density lipoprotein cholesterol (LDL-C) (149.7±26.4 versus 139.9±30.2 mg/dL, P<0.05), TBARS (0.5±0.2 versus 0.4±0.1 ?m/gHb, P<0.01), and CAT (245.9±46.9 versus 276.8±39.4 U/gHb). Melatonin administered for 2 months significantly improved antioxidative defense (increase in CAT activity, decrease in TBARS level) and lipid profile (decrease in LDL-C), and lowered blood pressure. We conclude that melatonin therapy may be of benefit for patients with MS, particularly with arterial hypertension. Further studies with higher doses of melatonin or prolonged supplementation are awaited. PMID:21138476

Koziróg, Marzena; Poliwczak, Adam Rafa?; Duchnowicz, Piotr; Koter-Michalak, Maria; Sikora, Joanna; Broncel, Marlena

2011-04-01

259

Milk production, intake, digestion, blood parameters, and ingestive behavior of cows supplemented with by-products from the biodiesel industry.  

PubMed

This study aimed to evaluate the intake, digestion, blood parameters, and feeding behavior of crossbred dairy cows (Holstein?×?Gir) managed on Panicum maximum Jacq. cv. Tanzania-1 and provided supplementation with groundnut cake, sunflower cake, or palm kernel cake (to replace soybean meal). Sixteen cows were randomly assigned in a Latin square design with four treatments and four experimental periods. The consumption of nutrients from the pasture did not vary between experimental treatments. Cows receiving the palm kernel cake supplement had a reduced crude protein, non-fibrous carbohydrate, and total digestible nutrient intake and an increase in the average consumption of ether extract. There was also a reduction in the digestibility of dry matter. The inclusion of supplements in the diet did not influence the average time spent eating, ruminating, or resting. The mean values of respiratory and heart rates showed thermal comfort during the trial period. There was a reduction in blood urea nitrogen with palm kernel cake supplementation, and the values of total protein, albumin, and glucose were also significantly different with this supplement. It is recommended that cakes of groundnut cake and sunflower cake seed be used for a total replacement of soybean meal supplements for lactating cows, but the use of palm kernel cake is not recommended. PMID:25319453

Neto, Severino Gonzaga; Oliveira, Ronaldo Lopes; de Lima, Francisco Helton Sá; de Medeiros, Ariosvaldo Nunes; Bezerra, Leilson Rocha; Viégas, Júlio; do Nascimento, Nilton Guedes; de Freitas Neto, Marcondes Dias

2015-01-01

260

Effect of pesticide residues on health and blood parameters of farm workers from rural Gadap, Karachi, Pakistan.  

PubMed

The samples were collected from 83 persons on 1 day (reading-I), 7 days (reading-II) and 30 days (reading-III) engaged in pesticide spraying in fourteen different fruit and vegetable farm stations located in the orchards of Gadap (rural area), Karachi-Pakistan. In the present study 26 workers out of 83 who were exposed to different pesticides (ie., cypermethrin, deltamethrin, polytrin-C, diazninon, monocrotophos, DDT and DDE) were compared with 25 healthy control persons. Different blood parameters were determined in comparison to control subjects. The results indicate some alterations in blood indices in allthe pesticide exposed persons, and only two persons from two different stations were affected severely and therefore their Hb, MCV MCHC, TLC, monocyte and neutrophil counts increased significantly. High lymphocyte count was noted in almost all the exposed persons. Platelets count was also found high in few persons but Hb, MCV, MCH, TLC, RBC and neutrophil counts significantly decreased in such persons. Exposure of multiple pesticides for prolong period has also affected the health of exposed persons and produced dermatological, hepatic, nephritic, respiratory and other clinical disorders reflecting the toxic effects of pesticides. Our findings indicate that indiscriminate use of pesticides in farming environments must be regularly assessed and farm workers must be trained for safe use of pesticides. PMID:20136060

Azmi, M Ahmed; Naqvi, S N H; Akhtar, Kehkashan; Moinuddin; Parveen, Shahida; Parveen, Rehana; Aslam, M

2009-09-01

261

The effect of serial in vitro haemodilution with maternal cerebrospinal fluid and crystalloid on thromboelastographic (TEG(®) ) blood coagulation parameters, and the implications for epidural blood patching.  

PubMed

Epidural blood patches may be used to treat post-dural puncture headache following accidental dural puncture in parturients. Their mode of action and the optimum volume of blood for injection remain controversial, with the interaction between injected blood and cerebrospinal fluid unknown. We aimed to establish the effects of serial haemodilution of whole blood with cerebrospinal fluid from 34 pregnant patients compared with serial haemodilution with Hartmann's solution, using the thromboelastogram. Haemodilution with either cerebrospinal fluid or Hartmann's solution had significant procoagulant and clot destabilising effects, enhanced with progressive haemodilution up to 30%. The effect of cerebrospinal fluid was greater compared with Hartmann's solution (p < 0.001). Cerebrospinal fluid led to a mean (95% CI) decrease in r-time by 2.4 (1.6-3.2) min, a decrease in k-time by 0.6 (0.4-0.8) min, an increase in alpha angle by 7.3 (5.5-9.0)°, and a decrease in maximum amplitude by 2.0 (0.6-3.4) mm. This may have implications for epidural blood patch, as success may be reduced near the time of dural puncture when cerebrospinal fluid leak is at its greatest, and large volumes of blood may be required to reduce haemodilution and clot destabilisation by cerebrospinal fluid. In addition, blood patching should be performed at the level of the dural puncture in order to ensure that the maximum volume of blood comes into contact with the cerebrospinal fluid. PMID:25428777

Armstrong, S; Fernando, R; Tamilselvan, P; Stewart, A; Columb, M

2015-02-01

262

Does blood transfusion affect pituitary gonadal axis and sperm parameters in young males with sickle cell disease?  

PubMed Central

Objective: We evaluated the effect of packed red cell transfusion (PCTx) on serum concentrations of gonadotropins luteinizing hormone and follicle-stimulating hormone (LH and FSH) and testosterone (T) levels and measured sperm parameters in young adults with sickle cell disease (SCD) on top-up transfusion (TTx) and those on exchange transfusion (ETx) regimen. Materials and Methods: Basal serum concentrations of FSH, LH, and T and semen parameters were evaluated before and 7 days after PCTx in 18 young adults with transfusion-dependent SCD, aged 20.7 ± 2.88 years. They had full pubertal development (Tanner's stage 5), and capacity to ejaculate. They were regularly transfused since early childhood. Chelation therapy was started early during the first 2 years of life using desferrioxamine and was replaced by deferasirox for the last 4-5 years. Ten patients were on TTx and eight were on ETx regimen. Results: PCTx significantly increased hemoglobin (Hb) from 8.5 ± 1.17 g/dl to 10.5 ± 0.4 g/dl, T from 12.3 ± 1.24 nmol/L to 14.23 ± 1.22 nmol/L and gonadotropins’ concentrations. Sperm parameters improved significantly after PCTx including: total sperm count from 87.4 ± 24.6 million/ml to 146.2 ± 51.25 million/ml, total progressive sperm motility (TPM) from 40.8 ± 11.1 million/ml to 93.4 ± 38.3 million/ml, rapid progressive sperm motility (RPM) progressive motility from 29.26 ± 8.75 million/ml to 67.4 ± 29 million/ml. After PCTx the total sperm count, TPM and RPM were significantly better in the ETx group versus the TTx group. Before and after PCTx, T concentrations were correlated significantly with sperm total count, volume, TPM and RPM (r = 0.53, 0.55, 0.42, and 0.38, respectively, P < 0.01). Hb concentrations were correlated significantly with sperm count, TPM, RPM, and % of sperms with normal morphology (r = 0.60, 0.69, 0.66, and 0.86, respectively, P < 0.001). Conclusion: Our study suggests that in males with SCD blood transfusion is associated with significant acute enhancement of sperm parameters and with increased concentrations of serum T, LH, and FSH. Improvement of sperm parameters were significantly better in the ETx group verses the TTx group. These “acute” effects on spermiogenesis are reached with an unknown mechanism/s and suggest a number of pathways that need further human and/or experimental studies. PMID:24381868

Soliman, Ashraf T.; Yasin, Mohamed; El-Awwa, Ahmed; Abdelrahman, Mohamed O.; De Sanctis, Vincenzo

2013-01-01

263

Effects of L-proline on the Growth Performance, and Blood Parameters in Weaned Lipopolysaccharide (LPS)-challenged Pigs  

PubMed Central

This trail was conducted to study the effect of L-proline on the growth performance, and blood parameter in the weaned lipopolysaccharide (LPS)-challenged pigs. Thirty six pigs (9.13±0.85 kg) were assigned randomly to dietary treatments in a 2×3 factorial arrangement in a 20-d growth assay. Factors were intraperitoneal injection with saline or LPS, and three dietary L-proline supplement levels (0%, 0.5%, or 1.0%). On d 10, blood samples were collected at 3 h after LPS (100 ?g LPS/kg body weight [BW]) or saline injection. On d 20 of the trial, all pigs were orally administrated D-xylose (0.1 g/kg BW) at 2 h, and blood samples were collected at 3 h after LPS or saline injection. As a result, dietary supplementation with 0.5% proline had a tendency to increase average daily gain (ADG) in piglets during d 10 to 20 (p = 0.088). Without LPS challenge, dietary supplementation with 1.0% proline had no effect on growth hormone (GH) concentrations on d 10 (p>0.05), but decreased it after LPS challenge (p<0.05). There was LPS challenge×proline interaction for GH concentrations on d 10 (p<0.05). Dietary supplementation with 1.0% proline decreased glucagon concentration on d 10 after LPS challenge (p<0.05). In addition, dietary supplementation with proline increased superoxide dismutase (SOD) activity significantly on d 10 and 20 (p<0.05), and 1.0% proline increased heat shock proteins-70 concentration on d 10 (p<0.05). Moreover, proline supplementation increased diamine oxidase (DAO) concentrations after LPS challenge (p<0.05). There was LPS challenge×proline interaction for DAO (p<0.05). Furthermore, dietary supplementation with 1.0% proline increased the D-xylose level when no LPS challenge (p<0.05). These results indicate that proline supplementation could improve growth performance, increase SOD activities, and has a positive effect on the gastrointestinal tract digestibility in early weaned pigs. PMID:25083109

Kang, Ping; Zhang, Lili; Hou, Yongqing; Ding, Binying; Yi, Dan; Wang, Lei; Zhu, Huiling; Liu, Yulan; Yin, Yulong; Wu, Guoyao

2014-01-01

264

Effects of L-proline on the Growth Performance, and Blood Parameters in Weaned Lipopolysaccharide (LPS)-challenged Pigs.  

PubMed

This trail was conducted to study the effect of L-proline on the growth performance, and blood parameter in the weaned lipopolysaccharide (LPS)-challenged pigs. Thirty six pigs (9.13±0.85 kg) were assigned randomly to dietary treatments in a 2×3 factorial arrangement in a 20-d growth assay. Factors were intraperitoneal injection with saline or LPS, and three dietary L-proline supplement levels (0%, 0.5%, or 1.0%). On d 10, blood samples were collected at 3 h after LPS (100 ?g LPS/kg body weight [BW]) or saline injection. On d 20 of the trial, all pigs were orally administrated D-xylose (0.1 g/kg BW) at 2 h, and blood samples were collected at 3 h after LPS or saline injection. As a result, dietary supplementation with 0.5% proline had a tendency to increase average daily gain (ADG) in piglets during d 10 to 20 (p = 0.088). Without LPS challenge, dietary supplementation with 1.0% proline had no effect on growth hormone (GH) concentrations on d 10 (p>0.05), but decreased it after LPS challenge (p<0.05). There was LPS challenge×proline interaction for GH concentrations on d 10 (p<0.05). Dietary supplementation with 1.0% proline decreased glucagon concentration on d 10 after LPS challenge (p<0.05). In addition, dietary supplementation with proline increased superoxide dismutase (SOD) activity significantly on d 10 and 20 (p<0.05), and 1.0% proline increased heat shock proteins-70 concentration on d 10 (p<0.05). Moreover, proline supplementation increased diamine oxidase (DAO) concentrations after LPS challenge (p<0.05). There was LPS challenge×proline interaction for DAO (p<0.05). Furthermore, dietary supplementation with 1.0% proline increased the D-xylose level when no LPS challenge (p<0.05). These results indicate that proline supplementation could improve growth performance, increase SOD activities, and has a positive effect on the gastrointestinal tract digestibility in early weaned pigs. PMID:25083109

Kang, Ping; Zhang, Lili; Hou, Yongqing; Ding, Binying; Yi, Dan; Wang, Lei; Zhu, Huiling; Liu, Yulan; Yin, Yulong; Wu, Guoyao

2014-08-01

265

Platelet procoagulant surface as an essential parameter for the in vitro evaluation of the blood compatibility of polymers  

Microsoft Academic Search

Thrombus formation at an artificial surface in contact with blood is the result of the interplay of two tightly linked biological systems, namely blood platelets and blood coagulation. While initiation of the overall process is thought to originate from proenzyme-enzyme conversions at the artificial surface, propagation of the process is only possible when a suitable phospholipid surface is available. The

T. Lindhout; R. Blezer; C. Maassen; V. Heijnen; C. P. M. Reutelingsperger

1995-01-01

266

Biochemical effects of Citrullus colocynthis in normal and diabetic rats.  

PubMed

Diabetes mellitus is one of the most common endocrine diseases. In UAE many traditional plants such as the Citrullus colocynthis (Handal) are used as antidiabetic remedies. The aim of this study was to examine the effect of the aqueous extract of the seed of C. colocynthis on the biochemical parameters of normal and streptozotocin (STZ)-induced diabetic rats. Diabetes mellitus was induced by a single intraperitoneal (60 mg/kg body wt1) injection of STZ. Normal and diabetic rats were fed with the plant extract daily by oral intubation for 2 weeks. Blood sample were collected at the beginning and end of the experiment for the measurement of biochemical parameters. The plasma level of alanine aminotranferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), lactic dehydrogenase (LDH) increased significantly after the onset of diabetes. Oral administration of the plant extract reduced the plasma level of AST and LDH significantly. However, the plant extract failed to reduce the increased blood level of GGT and ALP in diabetic rats. Blood urea nitrogen (BUN) increased significantly after the onset of diabetes. No significant difference was observed in the blood creatinine, K+, Na+, Ca2+ and P levels of normal and diabetic rats. The plant extract did not have any effect on BUN level, however, it caused an increase in the level of K+, Na+ in diabetic rats. In conclusion, oral administration of the aqueous extract of the C. colocynthis can ameliorate some of the toxic effects of streptozotocin. PMID:15362497

Al-Ghaithi, Fatma; El-Ridi, Mamdouh R; Adeghate, Ernest; Amiri, Mohamed H

2004-06-01

267

Morphological features and blood parameters of Weddell seal (Leptonychotes weddellii) mothers and pups during the breeding season.  

PubMed

We investigated the blood status of 9 Weddell seal mothers and 9 pups during the breeding season in a field study conducted from November to December 2004 at a breeding colony in Antarctica. The blood glucose and total cholesterol concentrations were higher in the pups than in the mothers. On the other hand, the blood urea nitrogen concentration was lower in the pups than in the mothers. Growth-associated depletion of blood triglyceride was observed in the pups and may have been due to the post-weaning fast. The results characterize the blood status of Weddell seals in relation to physiological adaptations for breeding. PMID:19346704

Sakamoto, Kentaro Q; Sato, Katsufumi; Naito, Yasuhiko; Habara, Yoshiaki; Ishizuka, Mayumi; Fujita, Shoichi

2009-03-01

268

Variability of biochemical parameters and radiation resistance of the seed progeny of Descurainia sophia and Lepidium apetalum under exposure to various factors  

Microsoft Academic Search

The physiological and biochemical characteristics of seedlings of Descurainia sophia (Fisch.) and Lepidium apetalum Wild. have been used as examples to demonstrate that long-term exposure of plant populations to environmental stress factors\\u000a causes adaptive changes in antioxidant and genomic systems that may considerably affect the radioresistance of seed progeny.\\u000a The resistance to additional acute irradiation of seed progeny of wild

I. A. Prokop’ev; A. N. Zhuravskaya; G. V. Filippova

2011-01-01

269

Weight gains, blood parameters, and fecal egg counts when meat-goat kids were finished on alfalfa, red clover, or orchardgrass pastures  

Technology Transfer Automated Retrieval System (TEKTRAN)

This experiment was conducted in 2005-2007 to evaluate weight gain, blood parameters associated with forage nutrient-use and anemia from gastrointestinal (GI) parasite infection, and fecal egg counts (FEC) patterns of meat goat kids finished on alfalfa (Medicago sativa L; ALF); red clover (Trifolium...

270

Arterial pressure variations as parameters of brain perfusion in response to central blood volume depletion and repletion  

PubMed Central

Rationale: A critical reduction in central blood volume (CBV) is often characterized by hemodynamic instability. Restoration of a volume deficit may be established by goal-directed fluid therapy guided by respiration-related variation in systolic- and pulse pressure (SPV and PPV). Stroke volume index (SVI) serves as a surrogate end-point of a fluid challenge but tissue perfusion itself has not been addressed. Objective: To delineate the relationship between arterial pressure variations, SVI and regional brain perfusion during CBV depletion and repletion in spontaneously breathing volunteers. Methods: This study quantified in 14 healthy subjects (11 male) the effects of CBV depletion [by 30 and 70 degrees passive head-up tilt (HUT)] and a fluid challenge (by tilt back) on CBV (thoracic admittance), mean middle cerebral artery (MCA) blood flow velocity (Vmean), SVI, cardiac index (CI), PPV, and SPV. Results: PPV (103 ± 89%, p < 0.05) and SPV (136 ± 117%, p < 0.05) increased with progression of central hypovolemia manifested by a reduction in thoracic admittance (11 ± 5%, p < 0.001), SVI (28 ± 6%, p < 0.001), CI (6 ± 8%, p < 0.001), and MCAVmean (17 ± 7%, p < 0.05) but not in arterial pressure. The reduction in MCAVmean correlated to the fall in SVI (R2 = 0.52, p < 0.0001) and inversely to PPV and SPV [R2 = 0.46 (p < 0.0001) and R2 = 0.45 (p < 0.0001), respectively]. PPV and SPV predicted a ?15% reduction in MCAVmean and SVI with comparable sensitivity (67/67% vs. 63/68%, respectively) and specificity (89/94 vs. 89/94%, respectively). A rapid fluid challenge by tilt-back restored all parameters to baseline values within 1 min. Conclusion: In spontaneously breathing subjects, a reduction in MCAVmean was related to an increase in PPV and SPV during graded CBV depletion and repletion. Specifically, PPV and SPV predicted changes in both SVI and MCAVmean with comparable sensitivity and specificity, however the predictive value is limited in spontaneously breathing subjects. PMID:24795652

Bronzwaer, Anne-Sophie G. T.; Stok, Wim J.; Westerhof, Berend E.; van Lieshout, Johannes J.

2014-01-01

271

Effect of graded physical load on the state of the liver from morphometric data and biochemical blood indices of rats against a background of hypokinesia  

NASA Technical Reports Server (NTRS)

Tests were conducted on 100 sexually immature inbred August and Wistar male rats in order to determine the effects hypokinesia, physical load and phenamine on the liver. Weight and linear dimension fell in hypokinesia; total serum protein lowered and aldolase and cholesterol and beta-lipoprotein levels rose. Blood sugar content rose and liver glycogen fell. Interlinear differences of these indices are found. Rehabilitated physical loading against hypokinesia background diminished and at times completely prevented its negative effect. Extent of correction depended on animal species. Evidence of genotypical conditionality of organism adaptation to physical load in hypokinesia was found.

Nikityuk, B. A.; Kogan, B. I.; Yermolyev, V. A.; Tindare, L. V.

1980-01-01

272

The Potential Biochemical Diagnosis Criteria and Therapeutic Effect Indexes: Sex Hormone Binding Globulin Levels and Free Androgen Index in Blood of Patients With Polycystic Ovary Syndrome  

ClinicalTrials.gov

The Investigators Collected 534 PCOS Patients as the Case Group,and 580 Infertile Women With Normal Ovulatory Cycle of the Control Group;; At the Same Time, the Investigators Continuedly Collect Cases to October 2012, and Totally Collected 579 Patients With PCOS Altogether;; 534 Patients in the Cases Group and 580 Women in the Control Group Received no Measures, While 579 Patients Received Drugs;; The Investigators Monitored Basic Indexes in Blood of All the Subjects in This Suvey,and Also Monitored Indexes of 579 Patients After Treatment.

2013-02-02

273

Cadmium, lead and mercury concentrations and their influence on morphological parameters in blood donors from different age groups from southern Poland.  

PubMed

Due to industrial development, environmental contamination with metals increases which leads to higher human exposure via air, water and food. In order to evaluate the level of the present exposition, the concentrations of metals can be measured in such biological materials as human blood. In this study, we assessed the concentrations of cadmium (Cd), mercury (Hg) and lead (Pb) in blood samples from male blood donors from southern Poland (Europe) born in 1994 (n=30) and between 1947 and 1955 (n=30). Higher levels of Pb were seen in the group of older men (4.48 vs 2.48?g/L), whereas the Hg levels were lower (1.78 vs 4.28?g/L). Cd concentrations did not differ between age groups (0.56?g/L). The levels of Cd and Pb in older donors were significantly correlated (Spearman R 0.5135). We also observed a positive correlation between the number of red blood cells (RBC) and Hg concentrations in the older group (Spearman R 0.4271). Additionally, we noted numerous correlations among morphological parameters. Based on our results, we can state that metals influence the blood morphology and their concentrations in blood vary among age groups. PMID:25457282

Janicka, Monika; Binkowski, Lukasz J; B?aszczyk, Martyna; Paluch, Joanna; Wojta?, W?odzimierz; Massanyi, Peter; Stawarz, Robert

2015-01-01

274

Activation of human eosinophils by monokines and lymphokines: source and biochemical characteristics of the eosinophil cytotoxicity-enhancing activity produced by blood mononuclear cells.  

PubMed

We and others have previously reported that human blood mononuclear cells release in culture certain substances that enhance the capacity of purified human blood eosinophils to kill the antibody-coated larvae of Schistosoma mansoni. The present study shows that this eosinophil cytotoxicity-enhancing activity (ECEA) is released by monocytes and T lymphocytes. Monocytes produce ECEA in resting and in LPS-stimulated cultures; T lymphocytes release such activity when stimulated by mitogens such as concanavalin A. Furthermore, the human monocytic line U-937 also releases ECEA-like activity when stimulated by LPS. The enhancing activity produced by monocytes has been partially characterized: it is sensitive to proteolysis by trypsin, relatively heat stable, and associated with molecules that have an apparent molecular weight of 14,000 to 65,000 daltons and isoelectric points of 3.8-3.9, 4.2, 4.5, 4.8-4.9. This shows that while ECEA produced by monocytes is heterogeneous in size and charge, it is probably different from interleukin 1. PMID:3875417

Lenzi, H L; Mednis, A D; Dessein, A J

1985-09-01

275

Comparative cardiovascular safety of risperidone and olanzapine, based on electrocardiographic parameters and blood pressure: A prospective open label observational study  

PubMed Central

Objective: To assess the cardiovascular safety of two commonly prescribed atypical antipsychotics risperidone (RSP) and olanzapine (OZP) in schizophrenic patients, using electrocardiography (ECG) and Blood Pressure (BP). Materials and Methods: This was a 10-week prospective open label, observational study, carried out in a newly diagnosed 64 schizophrenic patients receiving either RSP or OZP. RSP (n = 32) was started with dose of 2 mg/day and increased to 4 mg/day after 2 weeks, whereas OZP (n = 32) was started at a dose of 5 mg/day and was increased to 10 mg/day after 2 weeks. Heart rate (HR), ECG parameters (PR, RR, QRS, QT intervals and QTc and QTd) and BP (systolic and diastolic in supine and standing position) were recorded at baseline (before drug therapy)) and during follow-up visits at 2(I), 6(II) and 10(III) weeks. Results: In the RSP group, at II and III follow-ups, a significant increase in the HR (P = 0.018, P = 0.011 respectively) as well as in QTc (P = 0.025, P = 0.015, respectively) was observed when compared to the basal values. In the OZP group, diastolic BP was significantly decreased in standing position at II and III follow-ups (P = 0.045 and P = 0.024, respectively) compared to the basal values. When the two groups were compared with each other, no significant differences were observed in the changes of HR, PR, QRS, QT, RR, QT, QTd and SBP (supine and standing position); and DBP (supine position). However, DBP in standing position showed a significant decrease in the OZP group at II and III follow-up (P = 0.036 and P = 0.016, respectively) compared to the RSP group. Conclusions: Patients treated with OZP are at higher risk to develop postural hypotension as compared with RSP; hence RSP could be better tolerated by patients taking antihypertensive drugs as compared with OZP whereas OZP would have a safer cardiac profile. PMID:25298577

Choure, Balwant Kisanrao; Gosavi, Devesh; Nanotkar, Sanjay

2014-01-01

276

Effect of hempseed (Cannabis sativa L.) on performance, egg traits and blood biochemical parameters and antioxidant activity in laying Japanese Quail (Coturnix coturnix japonica).  

PubMed

1. This study was conducted to determine the effects of hempseed (HS) on performance, egg traits, serum lipid profile and antioxidant activity in Japanese quail (Coturnix coturnix japonica). 2. A total of 120 eight-week-old laying quail were divided into 4 experimental groups with 10 replicates. The treatments were as follows: (1) control diet (C, no HS in the diet); (2) 5% HS in the diet (HS5); (3) 10% HS in the diet (HS10); and (4) 20% HS in the diet (HS20). The quail were fed the experimental diets from 8 to 14 weeks of age. 3. There was no significant difference in body weight, feed intake and feed conversion ratio of the birds overall in the experiment. The egg production was not influenced by the HS contents in the diet; however the HS10 diet increased egg weight and egg-specific gravity. The carcass traits were not affected by the HS contents. 4. The serum triglyceride, cholesterol and the high density lipoprotein were not significantly altered; however, low density lipoprotein (LDL) concentration in HS-supplemented groups were lower than that of the C group. 5. The malondialdehyde, superoxide dismutase, catalase and nitrogen oxide concentrations were not significantly affected, but in the HS10 and HS20, glutathione peroxidase (GSH-Px) concentration was higher than in the C and HS5 groups. 6. The omega-3 fatty acid content of eggs increased linearly with increasing dietary HS content in the diet. 7. In conclusion, HS could be a potential feed and health benefit as a natural antioxidant in relation to decreasing serum LDL and increasing GSH-Px concentration in the liver of laying quail. PMID:25333932

Konca, Y; Yalcin, H; Karabacak, M; Kaliber, M; Durmuscelebi, F Z

2014-01-01

277

Unification of some biochemical methods of research in the pre- and post-flight periods  

NASA Technical Reports Server (NTRS)

The biochemical methods for determination of various parameters and factors during pre- and post-flight periods, as used by American and Soviet teams dealing with space flight medicine are compared. The emphasis is on the exchange of information on the study of the blood and urine content of space travelers before and after space flight. A series of electrolytic, enzymatic, and hormonal factors is discussed.

Tigranyan, R. A.

1980-01-01

278

Influence of bitches sex pheromones on the heart rate and other chosen parameters of blood flow in stud dogs (Canis familiaris).  

PubMed

The aim of this study was to verify the usefulness of the measurement of chosen cardiovascular activity parameters for examination of sex pheromones effects in male dogs. We evaluated the influence of the bitches' sexual pheromones (BSP) on heart rate (HR) with the use of a Holter monitor, and chosen parameters of blood flow in vessels of penis, testes and kidney, with color-coded and pulsed Doppler ultrasonography. We found that the BSP increased HR in all examined males even without showing any other signs of arousal. There were no changes in the RI (resistant index) and PI (pulsative index) in any trials during the pheromone presentation. The increase in blood flow intensity was noted in penile vessels but not in the testes and kidney. We concluded that measurement of flow intensity in the penis as well as the changes in heart rate as the male's reaction to the BSP can be useful in research concerning sex pheromones in dogs. PMID:22401977

Dzi?cio?, Micha?; Sta?czyk, Ewa; Noszczyk-Nowak, Agnieszka; Ni?a?ski, Wojciech; Ochota, Ma?gorzata; Kozdrowski, Roland

2012-12-01

279

Whole Blood Transcriptomics and Urinary Metabolomics to Define Adaptive Biochemical Pathways of High-Intensity Exercise in 50-60 Year Old Masters Athletes  

PubMed Central

Exercise is beneficial for a variety of age-related disorders. However, the molecular mechanisms mediating the beneficial adaptations to exercise in older adults are not well understood. The aim of the current study was to utilize a dual approach to characterize the genetic and metabolic adaptive pathways altered by exercise in veteran athletes and age-matched untrained individuals. Two groups of 50–60 year old males: competitive cyclists (athletes, n?=?9; VO2peak 59.1±5.2 ml·kg?1·min?1; peak aerobic power 383±39 W) and untrained, minimally active individuals (controls, n?=?8; VO2peak 35.9±9.7 ml·kg?1·min?1; peak aerobic power 230±57 W) were examined. All participants completed an acute bout of submaximal endurance exercise, and blood and urine samples pre- and post-exercise were analyzed for gene expression and metabolic changes utilizing genome-wide DNA microarray analysis and NMR spectroscopy-based metabolomics, respectively. Our results indicate distinct differences in gene and metabolite expression involving energy metabolism, lipids, insulin signaling and cardiovascular function between the two groups. These findings may lead to new insights into beneficial signaling pathways of healthy aging and help identify surrogate markers for monitoring exercise and training load. PMID:24643011

Mukherjee, Kamalika; Edgett, Brittany A.; Burrows, Harrison W.; Castro, Cecilia; Griffin, Julian L.; Schwertani, Adel Giaid; Gurd, Brendon J.; Funk, Colin D.

2014-01-01

280

Effect of Dietary Dried Berberis Vulgaris Fruit and Enzyme on Some Blood Parameters of Laying Hens Fed Wheat-Soybean Based Diets  

Microsoft Academic Search

2 Abstract: A study was conducted to evaluate dried berberry fruit (DBF) and enzyme on some blood parameters of laying hens. In a 5 * 2 completely randomized block design with factorial arrangement and 4 blocks as replicate, 5 levels of DBF (0.0, 0.5, 1.0, 1.5, and 2%) and 2 levels of NSP-degrading enzyme (0.0, and 0.05%) in wheat-soybean based

2006-01-01

281

Relationship between mean arterial pressure and parameters derived from blood flow velocity waveforms in the systemic circulation of fetal sheep.  

PubMed

In 8 fetal sheep under anesthesia, we examined the relationship between the fetal aorta blood flow velocity waveform and fetal mean arterial pressure after administration of norepinephrine and angiotensin II. It was hypothesized that the pulsatility index (PI) of the waveform would change during fetal hypertension. Measurements were performed using a 20-MHz Doppler transducer on the aorta directly beneath the diaphragm and a catheter inserted via the femoral artery into the abdominal aorta. Further instrumentation included a Doppler transducer and an electromagnetic flowmeter on the common umbilical artery and a catheter in the inferior vena cava. Fetal hypertension was induced by bolus administration of either norepinephrine or angiotensin II or by infusion of norepinephrine. With the Doppler transducer on the common umbilical artery, it was possible to study external iliac artery blood velocity waveforms in 4 fetal lambs, whereas aorta blood velocity waveforms were recorded in 7 fetal lambs. The measurements of external iliac artery PI and mean arterial pressure were characterized by a linear regression with a correlation coefficient of 0.72, whereas the measurements of aorta PI and mean arterial blood pressure were characterized by a linear regression with a correlation coefficient of 0.40. However, notable changes were observed in the aorta blood velocity waveform, which were not expressed by a substantial increase in the aorta PI. These changes can be described as a narrowing of the systolic peak and an earlier occurrence of the first dicrotic notch.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:8349170

Muijsers, G J; van Huisseling, H; Hasaart, T H

1993-01-01

282

Effect of exposure and withdrawal of 900-MHz-electromagnetic waves on brain, kidney and liver oxidative stress and some biochemical parameters in male rats.  

PubMed

Abstract Increasing use of mobile phones in daily life with increasing adverse effects of electromagnetic radiation (EMR), emitted from mobile on some physiological processes, cause many concerns about their effects on human health. Therefore, this work was designed to study the effects of exposure to mobile phone emits 900-MHz EMR on the brain, liver and kidney of male albino rats. Thirty male adult rats were randomly divided into four groups (10 each) as follows: control group (rats without exposure to EMR), exposure group (exposed to 900-MHz EMR for 1?h/d for 60?d) and withdrawal group (exposed to 900-MHz electromagnetic wave for 1?h/d for 60?d then left for 30?d without exposure). EMR emitted from mobile phone led to a significant increase in malondialdehyde (MDA) levels and significant decrease total antioxidant capacity (TAC) levels in brain, liver and kidneys tissues. The sera activity of alanine transaminase (ALT), aspartate aminotransferase (AST), urea, creatinine and corticosterone were significantly increased (p?biochemicals changes and oxidative stress in brain, liver and renal tissue of albino rats. These alterations were corrected by withdrawal. PMID:24712749

Ragy, Merhan Mamdouh

2014-04-01

283

Establishing blood group marker genes and comparing gross carcass parameters in normal and dystrophic New Hampshire chickens  

E-print Network

characteristic of all blood group loci in non-inbred populations. Using reagents specific for antigens of the B blood group system, Briles et al. (1957) tested a minimum of nine birds from each of seventy-three closed populations. At least seventy...- tern analysis, intra-line artificial inseminations were made using a single male p r line. This single male for each line was selected because of his unique cross-reaction pattern. Resulting progeny were then tested with the original refer- ence...

Hopkins, William Joseph

1980-01-01

284

A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level  

PubMed Central

Background In animal experiments, use of molecular hydrogen ( H2) has been regarded as quite safe and effective, showing benefits in multiple pathological conditions such as ischemia-reperfusion injury of the brain, heart, kidney and transplanted tissues, traumatic and surgical injury of the brain and spinal cord, inflammation of intestine and lung , degenerative striatonigral tissue and also in many other situations. However, since cerebral ischemia patients are in old age group, the safety information needs to be confirmed. For the feasibility of H2 treatment in these patients, delivery of H2 by inhalation method needs to be checked for consistency. Methods Hydrogen concentration (HC) in the arterial and venous blood was measured by gas chromatography on 3 patients, before, during and after 4% (case 1) and 3% (case2,3) H2 gas inhalation with simultaneous monitoring of physiological parameters. For a consistency study, HC in the venous blood of 10 patients were obtained on multiple occasions at the end of 30-min H2 inhalation treatment. Results The HC gradually reached a plateau level in 20 min after H2 inhalation in the blood, which was equivalent to the level reported by animal experiments. The HC rapidly decreased to 10% of the plateau level in about 6 min and 18 min in arterial and venous blood, respectively after H2 inhalation was discontinued. Physiological parameters on these 3 patients were essentially unchanged by use of hydrogen. The consistency study of 10 patients showed the HC at the end of 30-min inhalation treatment was quite variable but the inconsistency improved with more attention and encouragement. Conclusion H2 inhalation of at least 3% concentration for 30 min delivered enough HC, equivalent to the animal experiment levels, in the blood without compromising the safety. However, the consistency of H2 delivery by inhalation needs to be improved. PMID:22916706

2012-01-01

285

Effects of Processing of Starter Diets on Performance, Nutrient Digestibility, Rumen Biochemical Parameters and Body Measurements of Brown Swiss Dairy Calves  

PubMed Central

In order to investigate the effect of physical forms of starter diets on performance, weaning age, nutrient digestibility and rumen biochemical factors, 24 female of neonatal Brown Swiss calves (average body weight of 39.5±1.2 kg) were randomly assigned to three treatments. Dietary treatments were mashed (MS), pelleted (PS), and texturized (TS) starter using 8 calves from birth till 90 days of age in each treatment. Diets were formulated to be iso-nitrogenous with 21% crude protein. Based on the experimental results, calves that received PS and TS diets, had significant higher average daily gain (ADG) than those receiving MS (p<0.01). Dry matter intake in calves fed PS and TS was greater than calves fed MS (p<0.05), but there was no significant difference in feed efficiency. Treatments had no effect on initiation of rumination. Weaning age of calves in MS was longer than the other two treatments (p<0.05). Crude protein and organic matter digestibility in MS treated calves were lower than other treatments (p<0.05). No differences were observed in neutral detergent fiber (NDF) and ash digestibility among treatments (p>0.05). Ruminal pH was higher (p<0.01) in MS than the other groups, but ruminal ammonia (g/dl) concentration was not different among the treatments. Body measurements such as body length, pin width, hip width, pin to hip length, size of metacarpus and metatarsus bones, hip height, wither height, stomach size and heart girth were not significantly different among the treatments. Overall, it is concluded that starter diets in the form of pellet and texture can improve performance in neonatal Brown Swiss calves compared to the mashed form. PMID:25049653

Nejad, J. Ghassemi; Torbatinejad, N.; Naserian, A. A.; Kumar, S.; Kim, J. D.; Song, Y. H.; Ra, C. S.; Sung, K. I.

2012-01-01

286

Ergovaline in tall fescue and its effect on health, milk quality, biochemical parameters, oxidative status, and drug metabolizing enzymes of lactating ewes.  

PubMed

Ergovaline (EV) produced by symbiotic association of Epichloë coenophiala with tall fescue (Lolium arundinaceum) causes toxicoses in livestock. In this study, 16 lactating ewes (BW 76.0 ± 0.6 kg) were used to determine the effects of feeding endophyte-infected (FE+) or endophyte free (FE-) tall fescue hay on animal health and performances and to investigate the putative mechanisms of action of EV. The mean EV concentrations in FE+ and FE- diets were 497 ± 52 and <5 µg/kg DM, respectively. Decreased hay consumption and BW were observed in the FE+ group. Prolactin (PRL) concentrations decreased (P < 0.02) in the FE+ group from d 3 to 28 of the study compared to the FE- group, but no consequences were observed on milk quantity or quality. Skin temperature and the thermocirculation index were lower (P < 0.05) in the FE+ than in the FE- group from d 3 to 7, but this effect disappeared from d 14 to 28. Hematocrit, mineral and biochemical, and enzymatic analyses of plasma revealed no differences between the 2 groups. Measurement of oxidative damage and antioxidant enzyme activities revealed a decrease in the activities of plasma catalase (P < 0.05), kidney glutathione reductase and peroxidase and in kidney total glutathione and malondialdehyde contents (P < 0.02) in ewes fed FE+. Hepatic flavin monooxygenase enzyme activities decreased (P < 0.01) in ewes fed FE+, except for a marked increase in the demethylation of erythromycin. This activity is linked to cytochrome P4503A content and is known to be involved in ergot alkaloid metabolism. Glutathione S-transferase activity in the kidneys decreased (P < 0.02) in the FE+ group, whereas no difference was observed in uridine diphosphate-glucuronosyltransferase activity in the liver or kidneys. The reversibility of the effect of FE+ hay on skin temperature and the increase in erythromycin N-demethylase activity may contribute to the relative resistance of ewes to EV toxicity. PMID:25253811

Zbib, N; Repussard, C; Tardieu, D; Priymenko, N; Domange, C; Guerre, P

2014-11-01

287

Use of Audible and Chart-recorded Ultrasonography to Monitor Fetal Heart Rate and Uterine Blood Flow Parameters in Cattle  

Technology Transfer Automated Retrieval System (TEKTRAN)

The objective of the present study was to evaluate the use of audible chart-recorded doppler ultrasonography (DUS) to monitor both uterine blood flow and fetal heart rate (FHR) during pregnancy in dairy cattle. Possible applications of DUS include the monitoring of fetal distress when a pregnancy be...

288

PM2.5 EXPOSURE CHANGES HEART RATE VARIABILITY (HRV) AND BLOOD PARAMETERS IN STATE HIGHWAY PATROL TROOPERS  

EPA Science Inventory

Epidemiological studies show an association between ambient particulate matter PM and cardiovascular mortality. Panel and controlled exposure studies report PM-associated changes in HRV and blood factors involved in clotting and inflammation. We investigated the effects of in-veh...

289

Solidifying agent and processing of blood used for the larval diet affect screwworm (Diptera: Calliphoridae) life-history parameters  

Technology Transfer Automated Retrieval System (TEKTRAN)

The current artificial diet for mass rearing screwworm, Cochliomyia hominivorax, (Coquerel) (Diptera: Calliphoridae) larvae is a semi-solid medium consisting of dry whole bovine blood, poultry egg powder and a milk substitute mixed with a bulking and solidifying agent and water. To reduce the mass r...

290

Bio-efficacy comparison of herbal-methionine and DL-methionine based on performance and blood parameters of broiler chickens  

PubMed Central

This study was conducted to compare the bio-efficacy of herbal methionine (H-Met) relative to DL-methionine (DL-Met) on 160 “Ross 308” broiler chickens. DL-Met and H-Met were added to the basal diet in eight experimental treatments with three and four concentrations respectively in starter, grower and finisher period. Blood parameters which were measured at 24 and 42 days of age consisted of: serum proteins (total protein, albumin and globulin), serum uric acid, serum fats (low density lipoprotein, high density lipoprotein, triglyceride and cholesterol) and serum enzymes (alanine amino transaminase and aspartate amino transaminase). Completely randomized design, multi-exponential and multilinear regressions were used to determine bio-efficacy of H-Met in terms of performance and blood parameters of broilers. The results showed that supplemented methionine (Met) sources had no significant effect on blood parameters at 24 day of age. At 42 day of age the amounts of globulin and serum high density lipoprotein (HDL) increased with supplemented Met, (p < 0.05). Regression analysis revealed that H-Met was 55.00, 71.00, 78.00, 47.00, 58.00 and 73.00% as efficacious as DL-Met for body weight gain, feed intake, feed conversion ratio, albumin, globulin and high density lipoprotein criteria, respectively. The average of bio-efficacy of H-Met compared to DL-Met was 67.00% and 59.00% on average across performance criteria and blood criteria respectively and was 63.00% across these two criteria tested. The results of the present study indicated that H-Met can be administered as a new and a natural source of Met in poultry industry. PMID:25568699

Hadinia, Sheila; Shivazad, Mahmood; Moravej, Hossein; Alahyari-Shahrasb, Majid; Nabi, Mohammad Mehdi

2014-01-01

291

Effects of food processing and fibre content on the digestibility, energy intake and biochemical parameters of Blue-and-gold macaws (Ara ararauna L. - Aves, Psittacidae).  

PubMed

Considering the increased incidence of obesity and metabolic diseases in caged psittacines, the effect of fibre and food processing was evaluated in the Blue-and-gold macaw. Four food formulations (0%, 7%, 14% and 21% of sugarcane fibre) processed by pelleting or extrusion were studied, resulting in eight diets. To study digestibility, 48 macaws housed in pairs in cages was used in a block design. Subsequently, diets containing 0% or 21% sugarcane fibre, pelleted or extrude was fed for 4 months to evaluate energy intake and blood metabolites. A 2 × 2 × 2 (two fibre levels, two food processing methods and two genders) factorial arrangement with subplots (beginning and end) was used. When differences were detected in anova's F test, data were submitted to polynomial contrasts in the first experiment and to orthogonal contrasts in the second experiment (p < 0.05). Fibre addition reduced protein, fat and energy (p < 0.001) digestibility in both food processing. Pelleted foods presented higher dry matter digestibility and food metabolisable energy (ME) than the extruded ones (p < 0.05). Fibre addition or the type of processing did not change ME ingestion (p > 0.05). The macaws gained body weight (p < 0.05) regardless of the diet (p > 0.05), but females fed with the high-fibre diets did not gain weight (p > 0.05), suggesting a low food ME (12.5 kJ/g).The substitution of the original diet (sunflower seeds, fruits and cooked maize) by the experimental foods decreased the basal (12-h fast) concentrations of glucose, cholesterol and triglycerides (p < 0.001). The consumption of pelleted diets reduced serum glucose and cholesterol (p < 0.05). Results suggest that the pelleted diets were more beneficial and can be used to reduce blood metabolites related to metabolic disorders that are commonly observed in macaws. PMID:23627662

Veloso, R R; Sakomura, N K; Kawauchi, I M; Malheiros, E B; Carciofi, A C

2014-04-01

292

A longitudinal study to assess the impact of exercise on clinical, biochemical, and anthropometric parameters among the type 2 diabetes patients of South India  

PubMed Central

Aim: Aim of the study isto assess the scope of physical activity among type 2 diabetes mellitus (T2DM) patients residing in an urban area of SouthIndia. Materials and Methods: A longitudinal interventional study was conducted for a period of 8months (January 2014–August 2014) among patients of T2DM accessing the healthcare services at an Urban Health and Training Center (UHTC) of a Medical College located in Kancheepuram district, Tamil Nadu. Universal sampling method was employed and all diagnosed T2DM patients attending UHTC during the month of January were enrolled in the study provided they satisfied the inclusion and exclusion criteria. Overall 151 participants were considered for final analysis and all of them were subjected to indulge in moderate type of aerobic exercise in the form of brisk walking for 2.5 h spread over a period of 1 week, for the next 6months. Before and after the exercise regimen, cases were subjected to clinical evaluation, measurement of weight and waist circumference, and estimation of fasting and postprandial blood sugar; and the results were compared with the baseline estimates. The associations were tested using paired t-test for continuous (mean ± standard deviation ( SD)) variables. Results: Majority (86, 57%) of the diabetics were from the age group of 40–60 years with a definite female preponderance (82/151) in contrast to males (69/151). A significant reduction in both fasting and postprandial sugar was observed before and after the exercise. However, from the perspective of the weight and waist circumference, although a reduction was observed, the association was not statistically significant. Conclusion: The findings of the present longitudinal study suggest that compliance with moderate type of physical activity can significantly improve the fasting and postprandial blood sugar, apart from reduction in weight of T2DM patients. PMID:25625085

Sukla, Pradeep; Shrivastava, Saurabh RamBihariLal; Shrivastava, Prateek Saurabh

2015-01-01

293

[Biochemical diagnostics of fatal opium intoxication].  

PubMed

Biochemical diagnostics of fatal opium intoxication remains a topical problem in forensic medical science and practice. We investigated materials obtained in the course of forensic medical expertise of the cases of fatal opium intoxication. The study revealed significant differences between myoglobin levels in blood, urine, myocardium, and skeletal muscles. The proposed approach to biochemical diagnostics of fatal opium intoxication enhances the accuracy and the level of evidence of expert conclusions. PMID:23802303

Papyshev, I P; Astashkina, O G; Tuchik, E S; Nikolaev, B S; Cherniaev, A L

2013-01-01

294

EFFECTS OF CAPTURE ON BIOLOGICAL PARAMETERS IN FREE-RANGING BIGHORN SHEEP (OVIS CANADENSIS): EVALUATION OF NORMAL, STRESSED AND MORTALITY OUTCOMES AND DOCUMENTATION OF POSTCAPTURE SURVIVAL  

Microsoft Academic Search

Blood samples and physiological data were collected from 634 bighorn sheep (Otis canadensis) captured by four different methods between 1980 and 1986 in the western United States. These parameters were evaluated for selected physiological, biochemical and hematological values. Postcapture biological parameters were compared among bighorn sheep according to four different outcomes; normal, stressed or compromised, capture myopathy (CM) mortality, and

Michael D. Kock; Richard K. Clark; Charles E. Franti; David A. Jessup; John D. Wehausen

295

The Effect of Organic Solvents and Other Parameters on Trypsin-Catalyzed Hydrolysis of Na-Benzoyl-arginine-p-nitroanilide. A Project-Oriented Biochemical Experiment  

NASA Astrophysics Data System (ADS)

The study of enzymatic catalysis is a classical biochemistry experiment for undergraduate classes. We propose the utilization of the serine protease trypsin to discuss several parameters affecting enzyme catalysis. Hydrolysis of the chromogenic substrate Na -benzoyl-arginine-p-nitroanilide (BApNA) was followed by spectrophotometric monitoring. The optimal pH and temperature values were found to be 8.0 and 40 °C, respectively. Km and Vmax values were obtained by adjustment to Michaelis-Menten, Lineweaver-Burke, and Hanes equations. We then investigated the effect of organic solvents (a series of alcohols) on the hydrolysis of the chromogenic substrate. The reaction rate was reduced in the presence of methanol and further reduced by ethanol, 1-propanol, and 2-propanol, when compared to the data obtained with buffer. Finally the students were asked to measure the molar absorptivity of p-nitrophenol in the presence of the alcohols employed for the kinetic experiments. Thus they could learn that the value of this parameter varies with the solvent. These experiments were designed as a project-oriented approach to teach biochemistry methodologies and theoretical aspects of enzyme kinetics. They took about four months with four to six hours per week spent in the laboratory.

Correia, L. C.; Bocewicz, A. C.; Esteves, S. A.; Pontes, M. G.; Versieux, L. M.; Teixeira, S. M. R.; Santoro, M. M.; Bemquerer, M. P.

2001-11-01

296

Single and combined effects of vitamin C and oregano essential oil in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress condition.  

PubMed

This study was conducted to evaluate the effects of adding vitamin C (VC), oregano essential oil (OR), or their combination in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress (HS) condition (38 °C). One-day-old 240 male broilers were randomly assigned to four treatment groups, six replicates of ten birds each. The birds were fed with either a basal diet or a basal diet supplemented with either 200 mg L-ascorbic acid/kg of diet, 250 mg of oregano essential oil/kg of diet, or 200 mg L-ascorbic acid plus 250 mg of oregano essential oil/kg of diet. Average daily feed intake (ADFI), average daily gain (ADG), and feed conversion ratio (FCR) were obtained for 42 days of age and at the end of the experiment (day 42); birds were bled to determine some blood parameters and weighted for final body weight (BW). Feeding birds with diets supplemented with oregano essential oil and vitamin C in a single or combined form increased ADG (P?>?0.05). Also BW increased and feed efficiency decreased (P??0.05). Supplemental oregano essential oil and vitamin C in a combined form decreased the serum concentration of corticosterone, triglycerides, glucose, and MDA (P?blood parameters of broiler chicks reared under heat stress condition. PMID:25336108

Ghazi, Shahab; Amjadian, Tahere; Norouzi, Shokufeh

2014-10-22

297

Macro- and microrheological parameters of blood in patients with cerebral and peripheral atherosclerosis: the molecular change mechanisms after pentoxifylline treatment.  

PubMed

This study was designed to evaluate hemorheological changes in patients with cerebrovascular disease (CVD) and peripheral arterial disease (PAD) after 4 weeks of pentoxifylline therapy as well as to study red blood cell microrheological variables after the cell incubation with pentoxifylline and some phosphodiesterase (PDE) activity inhibitors. The patients with CVD (n = 50) and PAD (n = 33) were treated with pentoxifylline (400 mg, thrice a day) for 4 weeks. Before and after drug therapy the hemorheological measurements including plasma and whole blood viscosity, red blood cell aggregation (RBCA) and deformability (RBCD) were completed. In vitro study RBCs were incubated with: 1) Vinpocetine--inhibitor PDE-1, 10 ?M; 2) Rolipram--PDE-4, 10 ?M; 3) Isobutyl-methylxanthine (IBMX)--nonselective PDE inhibitor, 100 ?M and with pentoxifylline, 10 ?M The cell incubation was performed at 37 °C for 15 min. There were the positive changes of hemorheological profile after 4 weeks of the pentoxifylline therapy both in CVD and PAD patients. The marked RBCD changes were observed after the in vitro cell pentoxifylline treatment as well. Perhaps it is connected with the inhibition of the phosphodiesterase activity in RBCs. An application of drugs and chemicals that can inhibit the PDE activity resulted in RBCD rise and RBCA decrease. The experiments with the use of selective PDE inhibitors have revealed the similar red cell deformability changes. Vinpocetine increased RBCD significantly (p < 0.05). PDE-4 inhibitor--Rolipram stimulated RBCD by 15% (p < 0.05). Some more effective was IBMX. After cell incubation with it a significant rise of the deformability (by 27%; p < 0.05) was found. All drugs, having PDE activity decreased RBCA, but the most pronounced effect had Vinpocetine (50%; p < 0.05). Thus, administered pentoxifylline, daily (1200 mg), during four weeks improves hemorheological profile and especially its microrheological part as well as the blood transport capacity in subjects with cerebral and peripheral vascular disorders. It is most probably red cell microrheological control mechanisms may be associated with the phosphodiesterase activity alterations. PMID:22214714

Muravyov, Alexei V; Bulaeva, Svetlana V; Tikhomirova, Irina A; Zamishlayev, Andrey V; Uzikova, Ekaterina V; Miloradov, Mikhail Ju

2011-01-01

298

Effects of add-on nebivolol on blood pressure and glucose parameters in hypertensive patients with prediabetes.  

PubMed

In this multicenter trial, the effects of nebivolol added to an angiotensin-converting enzyme (ACE) inhibitor or angiotensin II receptor blocker (ARB) were assessed in patients with hypertension (diastolic blood pressure [DBP] 80-110 mm Hg) and prediabetes (fasting blood glucose 100-125 mg/dL and/or 2-hour oral glucose tolerance test [OGTT] 140-199 mg/dL). After a 4-week run-in period (in which lisinopril [10 mg/d] or losartan [50 mg/d] treatment was initiated), patients with DBP 90-110 mm Hg were randomized (2:2:1) to 12-week, double-blind treatment with nebivolol (n=223; 5-40 mg/d), hydrochlorothiazide (HCTZ; n=212; 12.5-25 mg/d), or placebo (n=102), titrated to achievement of 130/80 mm Hg. The primary outcome measure was DBP (last observation carried forward, intent to treat population); secondary measures included systolic blood pressure (SBP) and glucose levels. At baseline, overall mean values for body mass index, triglycerides, and high-density lipoprotein cholesterol were 32.3 kg/m(2) , 1.7 mmol/L, and 1.3 mmol/L, respectively. At week 12, nebivolol and placebo groups demonstrated a decrease of -9.4 and -5.0 mm Hg, respectively (P<.001) for DBP and -10.4 and -7.8 mm Hg for SBP (P=.147). The mean changes in area under the curve OGTT were 0.0 mg/dL (nebivolol), 6.9 mg/dL (HCTZ; P=.024 vs nebivolol), and -1.0 mg/dL (placebo). Adverse event-related discontinuation rates were 10.3%, 6.6%, and 2.0%, respectively. Nebivolol, added to an ACE inhibitor or ARB, provides additional blood pressure reduction with little or no effect on glucose metabolism in hypertensive patients with prediabetes. PMID:23551727

Deedwania, Prakash; Shea, John; Chen, Wei; Brener, Lillian

2013-04-01

299

A review of PAH exposure from the combustion of biomass fuel and their less surveyed effect on the blood parameters.  

PubMed

Many epidemiological studies from all over the world have reported that populations of rural and urban environments differ in their health issues due to the differences in the countrywide pollution pattern. In developing countries, various occupational cohorts and subsections of the population in urban and rural areas are routinely exposed to several environmentally widespread contaminants. Polycyclic aromatic hydrocarbons (PAHs) are a group of over hundred different compounds and have ubiquitous presence in rural and urban environments. Smoke from the combustion of biomass fuel contains a high concentration of carcinogenic PAHs, which are related with several human morbidities. The sources and types of biomass fuel are diverse and wide in distribution. Limited numbers of literature reports have focused the significant impact of PAHs on several components of blood, both in human and wildlife. The toxicity of PAHs to rapidly dividing cells (e.g., bone marrow cells) and other tissues is largely attributed to their reactive oxygenated metabolites, potential of causing oxidative stress, and the adducts of their metabolites with DNA. This review aims to encompass the blood-related effects of PAHs and associated human health risks-an aspect that needs further research-on the population of developing countries of the world in particular. PMID:25410307

Kamal, Atif; Cincinelli, Alessandra; Martellini, Tania; Malik, Riffat Naseem

2014-11-21

300

Continuous and noninvasive measurement of systolic and diastolic blood pressure by one mathematical model with the same model parameters and two separate pulse wave velocities.  

PubMed

There is keen interest in continuous and noninvasive blood pressure (BP) measurement. However, many technologies have a shortcoming of complex mechanical structure. In our study, two arterial pulses are acquired by photoplethysmography (PPG) at ear and toe in order to explore a new method of measuring BP by pulse wave velocity (PWV). We previously validated and reported a BP-PWV mathematical model with measurements from humans with no evidence of cardiovascular disease, but were only able to determine PWV related to diastolic blood pressure (DBP). In this paper, we propose methods of identifying pulse transmit time (PTT) in low, normal and high systolic blood pressure (SBP) conditions. By averaging the PTT's of incident wave and reflected wave for non-systematic error reduction, we obtain a PWV that is suitable for estimating SBP. SBP and DBP are estimated by two separate PWV's based on the previously calibrated models. Experimental measurements are conducted on 26 subjects (age 19 ± 1 and 60 ± 1) with no evidence of cardiovascular disease. The measurement errors (Mean Deviation = 2.16 mmHg (SBP) and 1.49 mmHg (DBP); Standard Deviation = 6.23 mmHg (SBP) and 6.51 mmHg (DBP)) satisfy the accuracy criteria of Association for the Advancement of Medical Instrumentation. The results verify that SBP and DBP can be estimated by one mathematical model with the same model parameters and two separate PWV's. PMID:22101758

Chen, Yan; Wen, Changyun; Tao, Guocai; Bi, Min

2012-04-01

301

Effect of Resveratrol on Hematological and Biochemical Alterations in Rats Exposed to Fluoride  

PubMed Central

We investigated the protective effects of resveratrol on hematological and biochemical changes induced by fluoride in rats. A total of 28 rats were divided into 4 groups: control, resveratrol, fluoride, and fluoride/resveratrol (n = 7 each), for a total of 21 days of treatment. Blood samples were taken and hematological and biochemical parameters were measured. Compared to the control group, the fluoride-treated group showed significant differences in several hematological parameters, including decreases in WBC, RBC, and PLT counts and neutrophil ratio. The group that received resveratrol alone showed a decrease in WBC count compared to the control group. Furthermore, in comparison to the control group, the fluoride group showed significantly increased ALT enzyme activity and decreased inorganic phosphorus level. The hematological and biochemical parameters in the fluoride?+?resveratrol treated group were similar to control group. In the fluoride?+?resveratrol group, resveratrol restored the changes observed following fluoride treatment, including decreased counts of WBC, RBC, and PLT, decreased neutrophil ratio and inorganic phosphorus levels, and elevated ALT enzyme activity. The present study showed that fluoride caused adverse effects in rats and that resveratrol reduced hematological and biochemical alterations produced by fluoride exposure. PMID:24995323

Atmaca, Nurgül; Y?ld?r?m, Ebru; Güner, Bayram; Kabakç?, Ruhi; Bilmen, Fatih Sultan

2014-01-01

302

Optimization of recovery patterns in common carp exposed to roundup using response surface methodology: evaluation of neurotoxicity and genotoxicity effects and biochemical parameters.  

PubMed

The present study is the first report on optimization of recovery conditions of fishes exposed to pesticides using response surface methodology-central composite rotatable design (RSM-CCRD). The sub-lethal toxicity bioassay of Roundup® (2 ppm ~10 percent LC??, 96 h) in common carp (1, 4, 9, 16, 25, 35 and 40 day) was investigated. After exposure for 16 days to Roundup®, some the fishes were introduced to herbicide-free water. The effects of four recovery parameters including time (5-25 d), temperature (18-26 °C), water exchange rate (WER, 10-30), and salinity (0-8 ppt) on the levels of biomarkers of genotoxicity (DNA damage), neurotoxicity (acetylcholinesterase activity (AChE)), and the serum alanine (ALT) and aspartate (AST) aminotransferase in plasma were studied. The polynomial equations were significantly fitted for all response variables with high R² values (>0.95), which revealed no indication of lack of fit. The optimum conditions for the maximum AChE activity (37.14 nmol/min/mg protein) and the minimum levels of DNA damage (8.00 percent tail DNA), ALT (27.0 IU/L) and AST (91.0 IU/L) were time of 20 d, temperature of 20 °C, WER of 25 and water salinity of 6 ppt. Thus, a promising improvement for the recovery trend of fishes exposed to Roundup® stress was obtained under the optimized conditions using RSM-CCRD. PMID:24094415

Gholami-Seyedkolaei, Seyed Jalil; Mirvaghefi, Alireza; Farahmand, Hamid; Kosari, Ali Asghar; Gholami-Seyedkolaei, Seyed Jalal; Gholami-Seyedkolaei, Seyed Jamal

2013-12-01

303

Safety evaluation of phytosterols in laying hens: effects on laying performance, clinical blood parameters, and organ development.  

PubMed

Phytosterols are intended for use as a novel food ingredient with plasma cholesterol-lowering activity. Although phytosterols are naturally present in the normal diet, daily consumption is insufficient to ensure plasma cholesterol-lowering levels. Therefore, phytosterols may be added to the diets to achieve the desired cholesterol-lowering activity. A subchronic laying hen safety study was conducted to examine if high-dose phytosterols could affect the safety of hens. Three hundred sixty 21-wk-old Hy-Line Brown laying hens were randomly assigned to 5 groups with 6 replicates of 12 birds each; after 3 wk, birds were fed diets supplemented with 0, 20, 80, 400, and 800 mg/kg of phytosterols for 12 wk. Throughout the study, clinical observations and laying performance were measured. At the end of the study, birds were subjected to a full postmortem examination: blood samples were taken for clinical pathology, selected organs were weighed, and specified tissues were taken for subsequent histological examination. No treatment-related changes that were considered to be of toxicological significance were observed. Therefore, a nominal phytosterol concentration of 800 mg/kg was considered to be the no-observed-adverse-effect level. PMID:24604846

Shi, S R; Shen, Y R; Chang, L L; Zhou, C J; Bo, Z; Wang, Z Y; Tong, H B; Zou, J M

2014-03-01

304

Effect of macroalgae enriched with microelements on egg quality parameters and mineral content of eggs, eggshell, blood, feathers and droppings.  

PubMed

This study presents results obtained from feeding experiment on laying hens, which were fed with the diet supplemented with two marine macroalgae: Enteromorpha prolifera and Cladophora sp., enriched with microelements [Cu(II), Zn(II), Co(II), Mn(II), Cr(III)]. The applicability of the preparation was tested on five experimental groups of laying hens and one control group. In the control group, microelements were supplemented in the inorganic form, whereas in experimental groups, Cu, Zn, Co, Mn and Cr were replaced by macroalgae enriched with a given microelement ion. During feeding experiment, weight of laying hens, weight of eggs, eggshell thickness and mineral content of blood, feathers, droppings, eggs content (separately yolk and egg white) and eggshell were measured. Also egg number was counted and microclimate (temperature and relative humidity) was monitored. Supplementing bio-metallic feed additives to the diet of laying hens resulted in higher microelement transfer to eggs and enhanced the colour of yolk. It was also found that the presence of Enteromorpha prolifera and Cladophora sp. in laying hens diet influenced advantageously eggs weight, eggshell thickness as well as body weight of hens. On the basis of these results, it could be concluded that Enteromorpha prolifera and Cladophora sp. enriched with microelement ions could be potentially used as mineral feed additives in laying hens feeding. PMID:21039928

Michalak, I; Chojnacka, K; Dobrza?ski, Z; Górecki, H; Zieli?ska, A; Korczy?ski, M; Opali?ski, S

2011-06-01

305

A method for generating a mouse model of stroke: evaluation of parameters for blood flow, behavior, and survival [corrected].  

PubMed

Stroke is one of the common causes of death and disability. Despite extensive efforts in stroke research, therapeutic options for improving the functional recovery remain limited in clinical practice. Experimental stroke models using genetically modified mice could aid in unraveling the complex pathophysiology triggered by ischemic brain injury. Here, we optimized the procedure for generating mouse stroke model using an intraluminal suture in the middle cerebral artery and verified the blockage of blood flow using indocyanine green coupled with near infra-red radiation. The first week after the ischemic injury was critical for survivability. The survival rate of 11% in mice without any treatment but increased to 60% on administering prophylactic antibiotics. During this period, mice showed severe functional impairment but recovered spontaneously starting from the second week onward. Among the various behavioral tests, the pole tests and neurological severity score tests remained reliable up to 4 weeks after ischemia, whereas the rotarod and corner tests became less sensitive for assessing the severity of ischemic injury with time. Further, loss of body weight was also observed for up 4 weeks after ischemia induction. In conclusion, we have developed an improved approach which allows us to investigate the role of the cell death-related genes in the disease progression using genetically modified mice and to evaluate the modes of action of candidate drugs. PMID:24737945

Park, Sin-Young; Marasini, Subash; Kim, Geu-Hee; Ku, Taeyun; Choi, Chulhee; Park, Min-Young; Kim, Eun-Hee; Lee, Young-Don; Suh-Kim, Haeyoung; Kim, Sung-Soo

2014-03-01

306

A Method for Generate a Mouse Model of Stroke: Evaluation of Parameters for Blood Flow, Behavior, and Survival  

PubMed Central

Stroke is one of the common causes of death and disability. Despite extensive efforts in stroke research, therapeutic options for improving the functional recovery remain limited in clinical practice. Experimental stroke models using genetically modified mice could aid in unraveling the complex pathophysiology triggered by ischemic brain injury. Here, we optimized the procedure for generating mouse stroke model using an intraluminal suture in the middle cerebral artery and verified the blockage of blood flow using indocyanine green coupled with near infra-red radiation. The first week after the ischemic injury was critical for survivability. The survival rate of 11% in mice without any treatment but increased to 60% on administering prophylactic antibiotics. During this period, mice showed severe functional impairment but recovered spontaneously starting from the second week onward. Among the various behavioral tests, the pole tests and neurological severity score tests remained reliable up to 4 weeks after ischemia, whereas the rotarod and corner tests became less sensitive for assessing the severity of ischemic injury with time. Further, loss of body weight was also observed for up 4 weeks after ischemia induction. In conclusion, we have developed an improved approach which allows us to investigate the role of the cell death-related genes in the disease progression using genetically modified mice and to evaluate the modes of action of candidate drugs. PMID:24737945

Park, Sin-Young; Marasini, Subash; Kim, Geu-Hee; Ku, Taeyun; Choi, Chulhee; Park, Min-Young; Kim, Eun-Hee; Lee, Young-Don; Suh-Kim, Haeyoung

2014-01-01

307

Genotoxic effects of heavy metal cadmium on growth, biochemical, cyto-physiological parameters and detection of DNA polymorphism by RAPD in Capsicum annuum L. – An important spice crop of India  

PubMed Central

The present study was designed to investigate the effects of cadmium (Cd) on biochemical, physiological and cytological parameters of Capsicum annuum L. treated with five different concentrations (20, 40, 60, 80 and 100 ppm) of the metal. Shoot–root length, pigment and protein content showed a continuous decrease with increasing Cd concentrations and the maximal decline was observed at the higher concentration. Proline content was found to be increased upto 60 ppm while at higher concentrations it gradually decreased. MDA content and chromosomal aberrations increased as the concentration increased. Additionally Random amplified polymorphic DNA (RAPD) technique was used for the detection of genotoxicity induced by Cd. A total of 184 bands (62 polymorphic and 122 monomorphic) were generated in 5 different concentrations with 10 primers where primer OPA-02 generated the highest percentage of polymorphism (52.63%). Dendrogram showed that control, R1 and R2 showed similar cluster and R4 and R5 grouped with R3 into one cluster, which showed that plants from higher doses showed much difference than the plants selected at mild doses which resemble control at the DNA level. This investigation showed that RAPD marker is a useful tool for evaluation of genetic diversity and relationship among different metal concentrations. PMID:25313282

Aslam, Rumana; Ansari, M.Y.K.; Choudhary, Sana; Bhat, Towseef Mohsin; Jahan, Nusrat

2014-01-01

308

Genotoxic effects of heavy metal cadmium on growth, biochemical, cyto-physiological parameters and detection of DNA polymorphism by RAPD in Capsicum annuum L. - An important spice crop of India.  

PubMed

The present study was designed to investigate the effects of cadmium (Cd) on biochemical, physiological and cytological parameters of Capsicum annuum L. treated with five different concentrations (20, 40, 60, 80 and 100 ppm) of the metal. Shoot-root length, pigment and protein content showed a continuous decrease with increasing Cd concentrations and the maximal decline was observed at the higher concentration. Proline content was found to be increased upto 60 ppm while at higher concentrations it gradually decreased. MDA content and chromosomal aberrations increased as the concentration increased. Additionally Random amplified polymorphic DNA (RAPD) technique was used for the detection of genotoxicity induced by Cd. A total of 184 bands (62 polymorphic and 122 monomorphic) were generated in 5 different concentrations with 10 primers where primer OPA-02 generated the highest percentage of polymorphism (52.63%). Dendrogram showed that control, R1 and R2 showed similar cluster and R4 and R5 grouped with R3 into one cluster, which showed that plants from higher doses showed much difference than the plants selected at mild doses which resemble control at the DNA level. This investigation showed that RAPD marker is a useful tool for evaluation of genetic diversity and relationship among different metal concentrations. PMID:25313282

Aslam, Rumana; Ansari, M Y K; Choudhary, Sana; Bhat, Towseef Mohsin; Jahan, Nusrat

2014-11-01

309

Acid-base, blood gas, and physiologic parameters during laparoscopy in the head-down position in white-tailed deer (Odocoileus virginianus).  

PubMed

The objective of this project was to evaluate the acid-base, blood gas, and physiologic parameters of white-tailed deer (Odocoileus virginianus) during laparoscopy in the head-down position. Eleven white-tailed does were captured and then immobilized with xylazine (6 mg/kg i.m.) and ketamine (7 mg/kg i.m.). The deer were intubated orotracheally and maintained with isoflurane in oxygen. The deer were positioned in dorsal recumbency and positive pressure ventilated. Heart rate (HR), arterial blood pressure, end-tidal carbon dioxide concentration (FE/CO2), and CO2 insufflation pressure were recorded every 5 min. Respiratory parameters, plasma electrolytes, and peak inspiratory pressure were measured immediately before tilting deer in the head-down position (45-55 degrees), 5 min after tilting, and immediately before the end of the procedure (while tilted). Butorphanol (0.05 mg/kg i.m.) was administered at the end of the procedure and yohimbine (0.2 mg/kg i.v.) administered before release. The deer weighed 52 kg (28-70 kg) [median (minimum-maximum)]. The peak inspiratory pressure in dorsal recumbency while still horizontal was 25 cm H2O (16-28 cm H2O), which increased to 29 cm H2O (18-46 cm H2O) after tilting (P = 0.02). PaO2, PaCO2, FE/CO2, and pH did not change after tilting in the head-down position or after insufflation. HR did not change during the anesthetic period. Mean arterial pressure did not change after tilting or abdominal insufflation, but decreased by the end of the anesthetic period (approximately 1 hr). Time from intubation to extubation was 117 min (72-170 min) (n = 5) and surgery time was 31 min (17-60 min; n = 10). We conclude that captured white-tailed deer have minimal derangements to acid-base, blood gas, or physiologic parameters during laparoscopy in the head-down position with abdominal insufflation, and thus this procedure may be performed safely in ventilated white-tailed deer. PMID:17312721

Posner, Lysa P; Woodie, J Brett; Curtis, Paul D; Erb, Hollis N; Gilbert, Robert; Adams, Wendy A; Gleed, Robin D

2005-12-01

310

Testicular, semen and blood parameters in adapted and nonadapted Bos taurus bulls in the semi-arid tropics.  

PubMed

Two experiments were conducted to evaluate differences in testicular, seminal and hematological characteristics in adapted (Senepol) and nonadapted (Holstein) Bos taurus bulls under the semi-arid environmental conditions of St. Croix, Virgin Islands (17 degrees N, 64 degrees W). In Experiment 1 mature, sexually-rested Senepol (n=10) and Holstein (n=9) bulls of similar age (37 months) and body weight (715 kg) and grazing on adjacent native pastures, were tested on the same day in July (28.8 degrees C mean ambient temperature, 81.5% humidity). Senepol bulls had lower (P<0.01) rectal temperatures (39.3 vs 40.0 degrees C) and higher (P<0.01) packed cell volumes (41.4 vs 35.2%) than Holstein bulls. Scrotal circumference was 3 cm larger (P<0.1) and testis tone firmer (P<0.001) in Senepol compared to Holstein bulls. Ejaculates, obtained by electroejaculation, contained 3.2x10(9) more spermatozoa with fewer abnormal tails and detached acrosomes in Senepol than in Holstein bulls (P<0.05). In Experiment 2, Senepol (n=42) and Holstein (n=30) bulls, representing 3 beef and 5 dairy farms, were evaluated during the summer (August/September) and winter (February/March). Again, scrotal circumference was larger (P<0.05) and testis tone firmer (P<0.001) in Senepol than in Holstein bulls, with no effect of season. Seminal fructose was higher (P<0.01) in Senepol than in Holstein bulls and decreased (P<0.01) during the winter collection. Blood plasma urea nitrogen and glucose were similar between breeds, but urea nitrogen was lower (P<0.01) during the summer. Significant (P<0.01) breed-by-age interactions were observed for the frequency of spermatozoa with protoplasmic droplets, decreasing in Holstein but not in Senepol bulls. The data point to differences between the adapted and nonadapted breed type in testicular and ejaculate characteristics, but also suggest that season has only a limited impact on bull reproductive function under the environmental conditions in St. Croix. PMID:16727320

Wildeus, S; Hammond, A C

1993-08-01

311

The safest parameters for FUS-induced blood-brain barrier opening without effects on the opening volume  

NASA Astrophysics Data System (ADS)

Acoustic cavitation has been identified as the main physical mechanism for the focused ultrasound (FUS) induced blood-brain barrier (BBB) opening. In this paper, the mechanism of stable cavitation (SC) and inertial cavitation (IC) responsible for BBB opening was investigated. Thirty-three (n=33) mice were intravenously injected with bubbles of 4-5 ?m in diameter. The right hippocampus was then sonicated using focused 1.5-MHz ultrasound and three different studies were carried out. First, pulse lengths (PLs) of 0.1, 0.5, 2, and 5 ms at 0.18- MPa peak rarefactional pressure with 5-Hz pulse repetition frequency (PRF) and 5-minute duration were used to identify the threshold of PL using SC. Second, the effects of the duty cycle and exposure time were investigated. Third, the BBB opening size was compared between the SC and the IC. In the case of IC-induced BBB opening, a burst sequence (3-cycles PL; 5-Hz burst repetition frequency (BRF); 30 s duration) at 0.45 MPa was applied. Passive cavitation detection was performed with each sonication to detect whether a broadband response was obtained, i.e., if IC occurred, during BBB opening. The properties of BBB opening were measured through MRI. The threshold of PL for BBB opening was identified between 0.1 and 0.5 ms using SC, but the BBB can be opened in few cycles using IC. The BBB opening volume and normalized intensity increased with the PL, but reached saturation when the PL was above 2 ms. Once the PL threshold was reached, the same exposure time induced a similar BBB opening volume, but longer sonication duration induced higher MR intensity. The duty cycle was found not to play an important role on the BBB opening. Comparable BBB opening volume (20-25 mm3) could be reached between long PL (7500 cycles, i.e., 5 ms) at 0.18 MPa and 3 cycles at 0.45 MPa. 3-kDa fluorescently tagged dextran may be able to diffuse to the parenchyma after IC-induced BBB opening at 0.45 MPa but not after SC-induced BBB opening at 0.18 MPa.

Tung, Yao-Sheng; Olumolade, Yemi; Wang, Shutao; Wu, Shih-Ying; Konofagou, Elisa E.

2012-11-01

312

Diagnostic value of biochemical biomarkers in malignant and non-malignant pericardial effusion.  

PubMed

The aim of this study was to examine the biochemical composition of pericardial effusions of different etiology and to evaluate the diagnostic utility of biochemical parameters and tumor markers to discriminate malignant from benign effusion. Pericardial and serum levels of biochemical parameters and tumor markers were compared in 105 patients who underwent pericardiocentesis and pericardioscopy with targeted epicardial biopsy. Etiologic diagnosis was based on pericardial fluid and epicardial biopsy analysis by cytology, histology, immunohistochemistry, microbiology and polymerase chain reaction. The total of 105 patients comprised 29 patients with malignant and 76 patients with non-malignant pericardial effusions (40 autoreactive, 28 viral, 5 postcardiotomy syndromes and 3 associated with systemic diseases). Malignant pericardial effusions had significantly higher pericardial fluid levels of the tumor markers CEA, CA 19-9, CA 72-4, SCC and NSE (p < 0.001, p = 0.002, p < 0.001, p = 0.004 and p < 0.001, respectively) as well as higher pericardial fluid hemoglobin (p < 0.001), pericardial fluid white blood cells (p = 0.003), pericardial fluid LDH (p < 0.001) and ratio of pericardial to serum LDH levels compared to benign effusions. None of the biochemical or cell-count parameters tested proved to be accurate enough for distinguishing malignant from benign effusions. However, measurement of pericardial CA 72-4 levels offered a high diagnostic accuracy for malignancy, particularly in bloody pericardial effusions. None of the biochemical parameters tested was useful for the discrimination of malignant from benign effusions. However, measurement of pericardial CA 72-4 levels in bloody pericardial effusions yielded a high diagnostic accuracy and thus offers the potential as a diagnostic tool to distinguish between malignant and benign effusions. PMID:22638889

Karatolios, Konstantinos; Pankuweit, Sabine; Maisch, Bernhard

2013-05-01

313

A Method for Choosing the Smoothing Parameter in a Semi-parametric Model for Detecting Change-points in Blood Flow  

PubMed Central

In a smoothing spline model with unknown change-points, the choice of the smoothing parameter strongly influences the estimation of the change-point locations, and the function at the change-points. In a tumor biology example, where change-points in blood flow in response to treatment were of interest, choosing the smoothing parameter based on minimizing generalized cross validation, GCV, gave unsatisfactory estimates of the change-points. We propose a new method, aGCV, that re-weights the residual sum of squares and generalized degrees of freedom terms from GCV. The weight is chosen to maximize the decrease in the generalized degrees of freedom as a function of the weight value, while simultaneously minimizing aGCV as a function of the smoothing parameter and the change-points. Compared to GCV, simulation studies suggest that the aGCV method yields improved estimates of the change-point and the value of the function at the change-point. PMID:24459325

Han, Sung Wan; Mesquita, Rickson C.; Busch, Theresa M.; Putt, Mary E.

2013-01-01

314

Effect of bioactive substances found in rapeseed, raspberry and strawberry seed oils on blood lipid profile and selected parameters of oxidative status in rats.  

PubMed

Rapeseed, strawberry and raspberry seed oils are a rich source of polyunsaturated fatty acids and antioxidants such as tocols, bioflavonoids and phytosterols. The aim of the study was to determine changes in the blood lipid profile of rats fed with rapeseed, strawberry and raspberry seed oils and their effects on selected parameters of oxidative status. The experiment was carried out on male Wistar rats. The oils were administered by oral gavage for 5 weeks once daily at the dose of about 0.8 ml per rat. Blood samples were taken before and after supplementation period. The activity of superoxide dismutase (SOD) and glutathione peroxidase (cGPx) was assessed in erythrocytes and contents of triglycerides (TG), total cholesterol, low-density fraction of cholesterol (LDL) and high-density fraction of cholesterol (HDL) were assessed in plasma. The experiment shows that oils supplemented in the diet for 5 weeks had no significant effect on the level of triglyceride (TG), total cholesterol as well as HDL and LDL fractions. Reduced activity of cGPX and SOD in the group of rats receiving raspberry and strawberry seed oils suggests that these native oils may contribute to oxidative stability (improves antioxidant status). Thus, strawberry and raspberry seed oils can be considered as special biological oils, which constitute potential nutraceuticals reducing oxidative stress. PMID:24121557

Pieszka, Marek; Tombarkiewicz, Barbara; Roman, Adam; Migda?, W?adys?aw; Niedzió?ka, Jerzy

2013-11-01

315

Effects of a carotid covered stent with a novel membrane design on the blood flow regime and hemodynamic parameters distribution at the carotid artery bifurcation.  

PubMed

We have recently developed a novel membrane design for carotid covered stents that prevents emboli while preserving the external carotid artery (ECA) branch flow. Our earlier in vitro studies have shown that this novel design can maintain more than 83 % of the original ECA flow and has the potential to considerably reduce the chance of emboli release as compared to bare metal stents. In the present study, utilizing computational fluid dynamics simulations and fluid-structure interaction analyses, we further investigated the influence of this novel covered stent on the blood flow regime and distribution of hemodynamic parameters at the carotid artery bifurcation and within the branches. Simulation results of the effect of the covered stent on the flow division at the carotid bifurcation were comparable with the earlier experimental results and further verified that this covered stent can considerably preserve the ECA flow. The results also showed that this covered stent may affect the flow regime and the distribution of hemodynamic parameters at the opening of the ECA branch and at the apex of the divider wall. These altered local hemodynamic characteristics may promote the post-stenting patency of the ECA branch. Evaluation of shear-induced platelet activation suggested that activation of platelets due to the blood flow through this membrane is unlikely. However, some slow-flow regions near the stent membrane around the ECA opening may induce platelet aggregation and thrombus formation. This study further demonstrated the potential of this novel covered-stent design for the treatment of carotid atherosclerotic stenosis. Future in vivo investigations of the biological effects and mechanical performance of this covered-stent design (e.g., its thrombogenicity potential and biocompatibility) are warranted. PMID:25370154

Kabinejadian, Foad; Cui, Fangsen; Su, Boyang; Danpinid, Asawinee; Ho, Pei; Leo, Hwa Liang

2015-02-01

316

Understanding red blood cell parameters in the context of the frailty phenotype: interpretations of the FIBRA (Frailty in Brazilian Seniors) study.  

PubMed

Frailty and anemia in the elderly appear to share a common pathophysiology associated with chronic inflammatory processes. This study uses an analytical, cross-sectional, population-based methodology to investigate the probable relationships between frailty, red blood cell parameters and inflammatory markers in 255 community-dwelling elders aged 65 years or older. The frailty phenotype was assessed by non-intentional weight loss, fatigue, low grip strength, low energy expenditure and reduced gait speed. Blood sample analyses were performed to determine hemoglobin level, hematocrit and reticulocyte count, as well as the inflammatory variables IL-6, IL-1ra and hsCRP. In the first multivariate analysis (model I), considering only the erythroid parameters, Hb concentration was a significant variable for both general frailty status and weight loss: a 1.0g/dL drop in serum Hb concentration represented a 2.02-fold increase (CI 1.12-3.63) in an individual's chance of being frail. In the second analysis (model II), which also included inflammatory cytokine levels, hsCRP was independently selected as a significant variable. Each additional year of age represented a 1.21-fold increase in the chance of being frail, and each 1-unit increase in serum hsCRP represented a 3.64-fold increase in the chance of having the frailty phenotype. In model II reticulocyte counts were associated with weight loss and reduced metabolic expenditure criteria. Our findings suggest that reduced Hb concentration, reduced RetAbs count and elevated serum hsCRP levels should be considered components of frailty, which in turn is correlated with sarcopenia, as evidenced by weight loss. PMID:25236441

Silva, João Carlos; de Moraes, Zélia Vieira; Silva, Conceição; de Barros Mazon, Silvia; Guariento, Maria Elena; Neri, Anita Liberalesso; Fattori, André

2014-01-01

317

Effect of sex and dietary organic zinc on growth performance, carcass traits, tissue mineral content, and blood parameters of broiler chickens.  

PubMed

Zinc (Zn) is an essential mineral for animal development and function. A study was carried out to evaluate the effect of sex and dietary organic zinc (OZ) on growth performance, carcass traits, tissue mineral content, and blood parameters of broiler chickens. A total of 240 1-day-old male and 240 female broiler chicks (Cobb × Cobb) were assigned to two dietary levels of OZ (2 × 2 factorial) with six replicates per treatment (20 birds/replicate pen). The OZ supplementation levels were 0 and 25 ppm. Results showed that OZ supplementation did not affect the growth performance of male and female broilers, but the males showed significantly better (P < 0.05) growth performance than females did. Similarly, OZ supplementation did not affect the thickness of both the back and thigh skin of male and female broilers; however, males had thicker skin than females. Dietary OZ supplementation did not affect collagen contents in the skin and meat samples. Male broilers had higher skin collagen contents than females, but no sex difference was found in meat collagen contents. OZ supplementation did not affect the shear force values of skin and meat samples. Male broilers had higher shear force values of back skin than females, but not in the meat samples. Dietary OZ supplementation increased (P < 0.05) the thigh meat Zn content in both sexes. The plasma Ca content was significantly (P < 0.05) increased by dietary OZ supplementation; however, other blood parameters were not affected by dietary OZ supplementation. Males had higher plasma glucose and cholesterol content than females. It is concluded that dietary OZ supplementation at the level of 25 ppm does not affect the growth performance and skin quality of broiler chickens but increases the Zn content in thigh meat and Ca content in plasma of broiler chickens. Male broilers had better growth performance and skin quality than females. PMID:22167309

Salim, H M; Lee, H R; Jo, C; Lee, S K; Lee, Bong Duk

2012-06-01

318

Cholinesterase activities and biochemical determinations in patients with prostate cancer: influence of Gleason score, treatment and bone metastasis.  

PubMed

Prostate cancer (PCa) is the sixth most common type of cancer worldwide. Cholinesterase is well known as having non-cholinergic functions such as cellular proliferation and differentiation, suggesting a possible influence of cholinesterase in tumorogenesis. Thus, the aim of this study was to investigate the whole blood acetylcholinesterase (AChE) and plasma butyrylcholinesterase (BChE) activities and some biochemical parameters in PCa patients. This study was performed in 66 PCa patients and 40 control subjects. AChE and BChE activities were determined in PCa patients and the influence of the Gleason score; bone metastasis and treatment in the enzyme activities were also verified. Furthermore, we also analyzed possible biochemical alterations in these patients. AChE and BChE activities decreased in PCa patients in relation to the control group and various biochemical changes were observed in these patients. Moreover, Gleason score, metastasis and treatment influenced cholinesterase activities and biochemical determinations. Our results suggest that cholinesterases activities and biochemical parameters are altered in PCa. These facts support the idea that the drop in the cholinesterase activity and the consequent increased amount of acetylcholine could lead to a cholinergic overstimulation and increase the cell proliferation in PCa. PMID:22560633

Battisti, Vanessa; Bagatini, Margarete D; Maders, Liési D K; Chiesa, Juarez; Santos, Karen F; Gonçalves, Jamile F; Abdalla, Fátima H; Battisti, Iara E; Schetinger, Maria R C; Morsch, Vera M

2012-06-01

319

Association between weight gain, blood parameters, and thyroid hormones and the development of ascites syndrome in broiler chickens.  

PubMed

The present study examined the association between thyroid hormones and the development of ascites on one hand and the ability to predict ascites from growth rate and hematocrit on the other hand. Ascites syndrome was induced in broiler chickens in two trials by exposing the chicks to low ambient temperature (Ta) and by supplying a pellet form of diet. Weight gain, hematocrit, hemoglobin, and plasma thyroxin (T4) and triiodothyronine (T3) concentrations were measured weekly for each bird, and comparisons were made between birds that eventually died from ascites and those that did not. Mortality from ascites amounted to 24.3 and 24.2% in Trials 1 and 2, respectively. Weight gain did not differ between ascitic and healthy chickens up to approximately 2 wk before death but was significantly lower in the ascitic broilers 1 to 2 wk before death. Hematocrit was significantly higher in broilers with ascites with the exception of ascitic broilers that died at the age of 7 wk (Trial 1). In ascitic broilers, T4 and T3 concentrations declined significantly during the week of death. The present findings raise the question of whether the association between low levels of thyroid hormones and the development of ascites is one of the physiological responses in the syndrome cascade, or whether the failure to maintain thyroid hormones concentration is one of the triggers of the syndrome initiation. This question requires further investigation. It can be concluded that a high rate of weight gain is not always a good predictor of ascites development. Hematocrit and thyroid hormones can provide a good indication but only during the last week of life, and not in all cases. None of these parameters, however, can predict the development of ascites at an early age. PMID:11469663

Luger, D; Shinder, D; Rzepakovsky, V; Rusal, M; Yahav, S

2001-07-01

320

Raised serum albumin in hip osteoarthrosis: a comparative study in women of some blood chemical parameters in aging and in cases of femoral neck fractures, osteoporotic vertebral crush fractures, and hip osteoarthrosis  

Microsoft Academic Search

Twelve blood parameters were studied in five groups of women totalling 120 subjects--group I: 26 blood donors (average age 45.2 years, range 23-66); group II: 18 patients with various cerebral, cardiovascular, or infectious illnesses (average age 79.9 years, range 66-92); group III: 28 patients with femoral neck fractures (average age 79.4 years, range 56-95); group IV: 12 patients with hip

C H Rapin; R Lagier

1988-01-01

321

Blood Components  

MedlinePLUS

Home > Learn About Blood > Blood Components Printable Version Blood Components How can one donation help multiple people? ... blood. The main transfusable blood components include: Whole Blood Whole blood contains red cells, white cells, and ...

322

Ameliorative Potential of Psidium guajava on Hemato-biochemical Alterations in Arsenic-exposed Wistar Rats.  

PubMed

The present study attempts to investigate the effects of Psidium guajava (P. guajava) when administered in combination with sodium arsenite @ 20 ppm in drinking water with the aim of achieving normalization of altered biochemical, hematological parameters suggestive of hepatic damage and depletion of inorganic arsenic following chronic arsenic exposure. Thirty adult Wistar rats were given 20 ppm arsenic for eight weeks along with hydro alcoholic leaf extract of P. guajava at a dose of 100 mg/kg body weight wt. (orally) (once daily for eight weeks). Arsenic exposure led to significant depletion of hemoglobin, red blood cells (RBC) and packed cell volume (PCV) but elevated leucocyte count (TLC). There was a significant increase (P<0.01/P<0.05) in serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphotase (ALP), acid phosphotase (ACP) and blood glucose whereas decrease in total protein level in arsenic-exposed untreated animals. The changes were accompanied by a significant elevation in blood and soft-tissue arsenic concentration. Co-administration of P. guajava was most effective not only in reducing arsenic-induced hematological and biochemical alterations but also in depleting arsenic from blood and soft tissues following arsenic exposure. We thus recommend combined leaf extract of P. guajava for achieving optimum effects of chelation therapy. PMID:22778508

Tandan, Neeraj; Roy, Manju; Roy, Sushovan

2012-05-01

323

Investigation of biochemical and haematological side-effects of cefquinome in healthy dogs.  

PubMed

In the present study, the effects of cefquinome, a 4th generation cephalosporin, on clinical, biochemical, haematological, and blood gas variables were investigated. Five healthy dogs were injected with cefquinome (1 mg/kg body weight, IM, daily) for 14 days. Negative effects of cefquinome on clinical, biochemical, and haematological variables were not observed, but it did change some blood gas variables. PMID:11205999

Maden, M; Tra?, B; Ba?, A L; Elmas, M; Yazar, E; Birdane, F M

2001-01-01

324

Evaluation of clinicopathological parameters in cattle grazing timber milkvetch.  

PubMed

The purpose of this study was to determine whether hematological and serum biochemical parameters could be used to diagnose poisoning in cattle grazing timber milkvetch (Astragalus miser var. serotinus), a nitropropanol-containing legume. Blood samples were obtained from 20 Hereford cattle on timber milkvetch range in southern British Columbia during 1992 and 1993. Relevant changes in blood parameters were not detected and the parameters could not be used to predict nitropropanol ingestion or intoxication. The same parameters were measured in dosing trials with cattle with rumen fistulas, but relevant changes were not detected. Succinate dehydrogenase is the key target in nitropropanol intoxication and an assay for this enzyme may provide a probe for diagnosis. PMID:8681281

Maricle, B; Tobey, J; Majak, W; Hall, J W

1996-03-01

325

Evaluation of clinicopathological parameters in cattle grazing timber milkvetch.  

PubMed Central

The purpose of this study was to determine whether hematological and serum biochemical parameters could be used to diagnose poisoning in cattle grazing timber milkvetch (Astragalus miser var. serotinus), a nitropropanol-containing legume. Blood samples were obtained from 20 Hereford cattle on timber milkvetch range in southern British Columbia during 1992 and 1993. Relevant changes in blood parameters were not detected and the parameters could not be used to predict nitropropanol ingestion or intoxication. The same parameters were measured in dosing trials with cattle with rumen fistulas, but relevant changes were not detected. Succinate dehydrogenase is the key target in nitropropanol intoxication and an assay for this enzyme may provide a probe for diagnosis. Images Figre 1. PMID:8681281

Maricle, B; Tobey, J; Majak, W; Hall, J W

1996-01-01

326

Life style and biochemical adaptation in Antarctic fishes  

Microsoft Academic Search

Respiration and metabolism are under investigation in Antarctic fish, in an effort to understand the interplay between ecology and biochemical and physiological processes. Fish of the dominant suborder Notothenioidei are red-blooded, except Channichthyidae (the most phyletically derived family), whose genomes retain transcriptionally inactive DNA sequences closely related to the ?-globin gene of red-blooded notothenioids and have lost the ?-globin locus.

Guido di Prisco

2000-01-01

327

Tissue Residues, Hematological and Biochemical Effects of Tilmicosin in Broiler Chicken  

PubMed Central

The aim of this study was to determine the blood and tissue concentrations profile and effect of tilmicosin on some hematological and biochemical parameters in broiler chicken. Fifty clinically healthy Hubbard chickens were orally administered 25?mg/kg BW of tilmicosin once daily for 5 consecutive days. Tissue residues of tilmicosin in slaughtered healthy chicken could not be detected by microbiological assay in all tested tissues except in lung (at 96 hours) and liver and kidneys (at 72 hours) after last administration. Tilmicosin caused temporary decrease in the RBCs and WBCs counts and has no effect on hemoglobin (Hb) and packed cell volume concentration (PCV). Also, the effect of tilmicosin on some biochemical parameters was as follows: the concentrations of creatinine, uric acid, electrolytes (sodium, potassium, and calcium), glucose, AST, ALT, ALP, and HDL-cholesterol in the serum of treated chicken did not change in response to the repeated oral administration of tilmicosin. There were only a temporary significant decrease in total protein and albumin concentrations and a significant increase in cholesterol and triglycerides concentrations. Chicken must not be slaughtered before 4 days from the stopping of tilmicosin administration. Tilmicosin makes temporary changes on hematological and biochemical parameters in broiler chicken. PMID:24808972

Elsayed, Mossad; Elkomy, Ashraf; Morad, Mohamed

2014-01-01

328

Serum Biochemical Response to Inhalant Anesthetics in New Zealand White Rabbits  

PubMed Central

Anesthetics can affect biochemical parameters, complicating the interpretation of laboratory results and perhaps leading to erroneous diagnoses. The present study was performed to characterize variations in selected rabbit biochemical parameters after inhalant anesthetics. Twenty New Zealand White rabbits were allocated to 2 treatment groups (n = 10 animals each), which received either halothane or isoflurane. Anesthesia was induced by using a face-mask, and rabbits were intubated for maintenance of anesthesia for 30 min. Blood samples were obtained before induction and at 1, 10, 30, 60, and 120 min and 24, 48, and 72 h after intubation. Serum cholesterol, triglycerides, albumin, total proteins, total bilirubin, sodium, potassium, chloride, calcium, and phosphorus concentrations were measured by using an autoanalyzer. Administration of halothane significantly increased serum triglyceride levels and decreased serum cholesterol, albumin, total protein, and potassium levels. Isoflurane administration increased serum triglyceride, phosphorus, and chloride concentrations and decreased serum calcium and potassium levels. Caution is required in interpreting data on serum biochemical parameters from rabbits anesthetized with halothane or isoflurane. PMID:20122317

Gil, Alfredo González; Silván, Gema; Villa, Alberto; Millán, Pilar; Martínez-Fernández, Leticia; Illera, Juan C

2010-01-01

329

Serum biochemical response to inhalant anesthetics in New Zealand white rabbits.  

PubMed

Anesthetics can affect biochemical parameters, complicating the interpretation of laboratory results and perhaps leading to erroneous diagnoses. The present study was performed to characterize variations in selected rabbit biochemical parameters after inhalant anesthetics. Twenty New Zealand White rabbits were allocated to 2 treatment groups (n = 10 animals each), which received either halothane or isoflurane. Anesthesia was induced by using a face-mask, and rabbits were intubated for maintenance of anesthesia for 30 min. Blood samples were obtained before induction and at 1, 10, 30, 60, and 120 min and 24, 48, and 72 h after intubation. Serum cholesterol, triglycerides, albumin, total proteins, total bilirubin, sodium, potassium, chloride, calcium, and phosphorus concentrations were measured by using an autoanalyzer. Administration of halothane significantly increased serum triglyceride levels and decreased serum cholesterol, albumin, total protein, and potassium levels. Isoflurane administration increased serum triglyceride, phosphorus, and chloride concentrations and decreased serum calcium and potassium levels. Caution is required in interpreting data on serum biochemical parameters from rabbits anesthetized with halothane or isoflurane. PMID:20122317

Gil, Alfredo González; Silván, Gema; Villa, Alberto; Millán, Pilar; Martínez-Fernández, Leticia; Illera, Juan C

2010-01-01

330

Effects of dietary selenium and vitamin E on immune response and biological blood parameters of broilers reared under thermoneutral or heat stress conditions.  

PubMed

A study was conducted using 360 broiler chickens to evaluate the effects of dietary vitamin E (0, 125 and 250 mg/kg), selenium (Se, 0, 0.5 and 1 mg/kg), or their different combinations on immune response and blood biological parameters of broilers raised under either thermoneutral (TN, 23.9 °C constant) or heat stress (HS, 23.9 to 37 °C cycling) conditions. Humoral immunity was assessed by intravenous injection of 7% sheep red blood cell (SRBC) followed by evaluation of serum for antibody titers in primary and secondary responses. Heterophil to lymphocyte (H/L) ratio also determined as an indicator of stress. Furthermore, at the end of the experiment, birds were bled for determination of some biological parameters. There was a significant reduction in body weight and feed intake, but the feed conversion ratio increased when the birds were exposed to HS (P<0.05). Body weight and feed intake were not influenced significantly by dietary vitamin E and Se (P>0.05), whereas feed conversion was improved significantly by 125 mg/kg vitamin E (P<0.05). The liver and lymphoid organ weights as well as IgM and IgG, antibody titers for primary and secondary antibody responses to SRBC were reduced significantly under HS (P<0.05). Heat stress also resulted in a significant increase in H/L ratio (P<0.05). Dietary vitamin E resulted in improvement of primary and secondary antibody responses both in TN and HS broilers (P<0.05). The HS birds also showed an improved antibody titer in secondary response with high concentration of Se (P<0.05). Vitamin E and Se had interactive effects on anti-SRBC titers; however, no consistent differences were found between dietary levels during the study. The H/L ratio decreased by feeding vitamin E at both levels either under HS or TN conditions (P?

Habibian, Mahmood; Ghazi, Shahab; Moeini, Mohammad Mehdi; Abdolmohammadi, Alireza

2014-07-01

331

Effects of dietary selenium and vitamin E on immune response and biological blood parameters of broilers reared under thermoneutral or heat stress conditions  

NASA Astrophysics Data System (ADS)

A study was conducted using 360 broiler chickens to evaluate the effects of dietary vitamin E (0, 125 and 250 mg/kg), selenium (Se, 0, 0.5 and 1 mg/kg), or their different combinations on immune response and blood biological parameters of broilers raised under either thermoneutral (TN, 23.9 °C constant) or heat stress (HS, 23.9 to 37 °C cycling) conditions. Humoral immunity was assessed by intravenous injection of 7 % sheep red blood cell (SRBC) followed by evaluation of serum for antibody titers in primary and secondary responses. Heterophil to lymphocyte (H/L) ratio also determined as an indicator of stress. Furthermore, at the end of the experiment, birds were bled for determination of some biological parameters. There was a significant reduction in body weight and feed intake, but the feed conversion ratio increased when the birds were exposed to HS ( P < 0.05). Body weight and feed intake were not influenced significantly by dietary vitamin E and Se ( P > 0.05), whereas feed conversion was improved significantly by 125 mg/kg vitamin E ( P < 0.05). The liver and lymphoid organ weights as well as IgM and IgG, antibody titers for primary and secondary antibody responses to SRBC were reduced significantly under HS ( P < 0.05). Heat stress also resulted in a significant increase in H/L ratio ( P < 0.05). Dietary vitamin E resulted in improvement of primary and secondary antibody responses both in TN and HS broilers ( P < 0.05). The HS birds also showed an improved antibody titer in secondary response with high concentration of Se ( P < 0.05). Vitamin E and Se had interactive effects on anti-SRBC titers; however, no consistent differences were found between dietary levels during the study. The H/L ratio decreased by feeding vitamin E at both levels either under HS or TN conditions ( P < 0.05). The serum concentrations of glucose, triglycerides, total cholesterol, and LDL-cholesterol were increased but serum HDL-cholesterol decreased in HS broilers ( P < 0.05).

Habibian, Mahmood; Ghazi, Shahab; Moeini, Mohammad Mehdi; Abdolmohammadi, Alireza

2014-07-01

332

Red Cell Distribution Width and Other Red Blood Cell Parameters in Patients with Cancer: Association with Risk of Venous Thromboembolism and Mortality  

PubMed Central

Background Cancer patients are at high risk of developing venous thromboembolism (VTE). Red cell distribution width (RDW) has been reported to be associated with arterial and venous thrombosis and mortality in several diseases. Here, we analyzed the association between RDW and other red blood cell (RBC) parameters with risk of VTE and mortality in patients with cancer. Methods RBC parameters were measured in 1840 patients with cancers of the brain, breast, lung, stomach, colon, pancreas, prostate, kidney; lymphoma, multiple myeloma and other tumor sites, that were included in the Vienna Cancer and Thrombosis Study (CATS), which is an ongoing prospective, observational cohort study of patients with newly diagnosed or progressive cancer after remission. Primary study outcome is occurrence of symptomatic VTE and secondary outcome is death during a maximum follow-up of 2 years. Results During a median follow-up of 706 days, 131 (7.1%) patients developed VTE and 702 (38.2%) died. High RDW (>16%) was not associated with a higher risk of VTE in the total study cohort; in competing risk analysis accounting for death as competing variable the univariable subhazard ratio (SHR) was 1.34 (95% confidence interval [CI]: 0.80–2.23, p?=?0.269). There was also no significant association between other RBC parameters and risk of VTE. High RDW was associated with an increased risk of mortality in the total study population (hazard ratio [HR, 95% CI]: 1.72 [1.39–2.12], p<0.001), and this association prevailed after adjustment for age, sex, hemoglobin, leukocyte and platelet count (HR [95% CI]: 1.34 [1.06–1.70], p?=?0.016). Conclusions RDW and other RBC parameters were not independently associated with risk of VTE in patients with cancer and might therefore not be of added value for estimating risk of VTE in patients with cancer. We could confirm that high RDW is an independent predictor of poor overall survival in cancer. PMID:25347577

Riedl, Julia; Posch, Florian; Königsbrügge, Oliver; Lötsch, Felix; Reitter, Eva-Maria; Eigenbauer, Ernst; Marosi, Christine; Schwarzinger, Ilse; Zielinski, Christoph; Pabinger, Ingrid; Ay, Cihan

2014-01-01

333

Initial blood storage experiment  

NASA Technical Reports Server (NTRS)

The possibility of conducting experiments with the formed elements of the blood under conditions of microgravity opens up important opportunities to improve the understanding of basic formed element physiology, as well as, contribution to improved preservation of the formed elements for use in transfusion. The physiological, biochemical, and physical changes of the membrane of the erythrocyte, platelet, and leukocyte was studied during storage under two specific conditions: standard blood bank conditions and microgravity, utilizing three FDA approved plastic bags. Storage lesions; red cell storage on Earth; platelet storage on Earth; and leukocyte storage Earth were examined. The interaction of biomaterials and blood cells was studied during storage.

Surgenor, Douglas MACN.

1988-01-01

334

Biochemical transformation of coals  

DOEpatents

A method of biochemically transforming macromolecular compounds found in solid carbonaceous materials, such as coal is provided. The preparation of new microorganisms, metabolically weaned through challenge growth processes to biochemically transform solid carbonaceous materials at extreme temperatures, pressures, pH, salt and toxic metal concentrations is also disclosed. 7 figs.

Lin, M.S.; Premuzic, E.T.

1999-03-23

335

Biochemical transformation of coals  

DOEpatents

A method of biochemically transforming macromolecular compounds found in solid carbonaceous materials, such as coal is provided. The preparation of new microorganisms, metabolically weaned through challenge growth processes to biochemically transform solid carbonaceous materials at extreme temperatures, pressures, pH, salt and toxic metal concentrations is also disclosed.

Lin, Mow S. (Rocky Point, NY); Premuzic, Eugene T. (East Moriches, NY)

1999-03-23

336

Saturated, omega-6 and omega-3 dietary fatty acid effects on the characteristics of fresh, frozen-thawed semen and blood parameters in rams.  

PubMed

The aim of this study was to investigate the effects of several dietary fatty acids (FAs) on semen quality and blood parameters in rams. We gave diet-supplemented treatments (35 g day(-1)  ram(-1) ) by C16:0 (palm oil), C18:2 [sunflower oil (SO)] and an n-3 source [fish oil (FO)] to 12 rams, who were fed for 15 weeks during their breeding season. Semen was collected once per week. Semen samples were extended with Tris-based cryoprotective diluents, then cooled to 5 °C and stored in liquid nitrogen. Positive responses were seen with FO after 4 weeks. The mean prefreezing semen characteristics improved with the intake of FO (P < 0.05). Interestingly, maximum sperm output in FO was achieved 7.5 × 10(9) when compared to palm oil 5.3 × 10(9) . Rams that received FO had the highest total testosterone concentrations (11.3 ng ml(-1) for FO, 10.8 ng ml(-1) for SO and 10.2 ng ml(-1) for palm oil) during the experiment (P < 0.05). FO also improved the rams' sperm characteristics after thawing (P < 0.05). Although C16:0 is a major saturated FA in ram sperm and all rams have been fed isoenergetic rations, the unique FAs of FO improved fresh semen quality and freezing ability compared to other oils. PMID:23157190

Esmaeili, V; Shahverdi, A H; Alizadeh, A R; Alipour, H; Chehrazi, M

2012-11-16

337

Formation of secondary messengers by blood-formed elements in low-power laser irradiation  

NASA Astrophysics Data System (ADS)

Irradiation of heparinized rat blood by He-Ne laser light ((lambda) - 632.8 nm, power density - 5 mW/cm2) during 15 or 30 min was performed in vitro experiments. The complex of biochemical parameters of erythrocytes, plasma and cytochemical parameters of polymorphonuclear leucocytes was studied. Laser irradiation was stated to cause different metabolic changes in red blood cells and neutrophils depending on the dose. In both doses of irradiation glucose-6-phosphate dehydrogenase activity lowers in erythrocytes, succinate dehydrogenase activity and lysosomal cationic proteins content increase in neutrophils. Stimulation of oxygen active forms production in cellular membranes of blood formed elements results in plasma malonic dialdehyde level increase and in the change of the balance between primary and secondary lipid peroxidation products. Cooperative interaction between different blood cells in the process of realization of system response to laser exposure is supposed to exist.

Brill, Gregory E.; Proshina, Olga V.; Zhigalina, Valentina N.; Filimonovskaya, Lyudmila S.; Romanova, Tatyana P.; Petrisheva, Svetlana G.; Zolotarjova, Tamara M.

1995-05-01

338

BIOCHEMICAL ADAPTATION KENNETH B. STOREY  

E-print Network

to perturbation by multiple environmental parameters--for example, temperature, pressure, pH, ionic strength organisms are ectotherms (cold- blooded) and, hence, a change in environmental tempera- ture means a change- ity of membranes. Living within our mammalian bodies, humans do not often fully appreciate

Storey, Kenneth B.

339

Potential biochemical markers for infantile autism  

Microsoft Academic Search

Biochemical markers are crucial to the development of early diagnosis of infantile autism. The blood concentrations of neuroanalytes\\u000a epinephrine, norepinephrine, dopamine, and serotonin were elevated in autistic subjects (n = 13) as compared to normal controls (n = 10). Autistic subjects had peptide patterns (peaks I-V, Sephadex G-25) that were different from those of normal controls.\\u000a Methionine-enkephalin has been tentatively

Porn P. Israngkun; Howard A. I. Newman; Suman T. Patel; Valentine A. Duruibe; Hussein Abou-Issa

1986-01-01

340

Hematologic and biochemical reference intervals for specific pathogen free 6-week-old Hampshire-Yorkshire crossbred pigs  

PubMed Central

Background Hematologic and biochemical reference intervals depend on many factors, including age. A review of the literature highlights the lack of reference intervals for 6-wk-old specific pathogen free (SPF) Hampshire-Yorkshire crossbred pigs. For translational research, 6-wk-old pigs represent an important animal model for both human juvenile colitis and diabetes mellitus type 2 given the similarities between the porcine and human gastrointestinal maturation process. The aim of this study was to determine reference intervals for hematological and biochemical parameters in healthy 6-wk-old crossbred pigs. Blood samples were collected from 66 clinically healthy Hampshire-Yorkshire pigs. The pigs were 6 wks old, represented both sexes, and were housed in a SPF facility. Automated hematological and biochemical analysis were performed using an ADVIA 120 Hematology System and a Cobas 6000 C501 Clinical Chemistry Analyzer. Results Reference intervals were calculated using both parametric and nonparametric methods. The mean, median, minimum, and maximum values were calculated. Conclusion As pigs are used more frequently as medical models of human disease, having reference intervals for commonly measured hematological and biochemical parameters in 6-wk-old pigs will be useful. The reference intervals calculated in this study will aid in the diagnosis and monitoring of both naturally occurring and experimentally induced disease. In comparison to published reference intervals for older non SPF pigs, notable differences in leukocyte populations, and in levels of sodium, potassium, glucose, protein, and alkaline phosphatase were observed. PMID:24410946

2014-01-01

341

Treatment of rats with the Pelargonium sidoides extract EPs 7630 has no effect on blood coagulation parameters or on the pharmacokinetics of warfarin.  

PubMed

Umckaloabo is a herbal drug for the treatment of respiratory tract infections. It contains an aqueous ethanolic extract from roots of Pelargonium sidoides DC (EPs) 7630) as the active ingredient. Polymeric polyphenols and coumarins have been identified as the principal ingredients of EPs 7630. In view of the coumarin content, it has been suggested that the administration of Umckaloabo could possibly be associated with an increased risk of bleeding. This study, therefore, investigated whether a change in blood coagulation parameters or an interaction with coumarin-type anticoagulants occurred after administration of EPs 7630 to rats. No effect on thromboplastin time (TPT), partial TPT (PTPT) or thrombin time (TT) was observed after oral administration of EPs 7630 (10, 75, 500 mg/kg) for 2 weeks, while treatment with warfarin (0.05 mg/kg) for the same period resulted in significant changes in TPT and PTPT. If EPs 7630 (500 mg/kg) and warfarin (0.05 mg/kg) were given concomitantly, the anticoagulant action of warfarin was not influenced. Similarly, the pharmacokinetics of warfarin were unchanged after pre-treatment with EPs 7630 for 2 weeks. Coumarin-type anticoagulants inhibit the synthesis of vitamin K-dependent coagulation factors via an identical mechanism in rat and man, and have a similar pattern of metabolism in both species. Moreover, as the coumarins so far identified in EPs 7630 do not posses the structural characteristics needed for anticoagulant activity, it appears unlikely that an increased tendency to haemorrhage arises in patients after intake of Umckaloabo. PMID:17188479

Koch, E; Biber, A

2007-01-01

342

Perioperative blood management  

PubMed Central

Perioperative anaemia and allogenic blood transfusion (ABT) are known to increase the risk of adverse clinical outcomes. The quality, cost and availability of blood components are also major limitations with regard to ABT. Perioperative patient blood management (PBM) strategies should be aimed at minimizing and improving utilization of blood components. The goals of PBM are adequate preoperative evaluation and optimization of haemoglobin and bleeding parameters, techniques to minimize blood loss, blood conservation technologies and use of transfusion guidelines with targeted therapy. Attention to these details can help in cost reduction and improved patient outcome.

Manjuladevi, M; Vasudeva Upadhyaya, KS

2014-01-01

343

Blood pressure  

MedlinePLUS Videos and Cool Tools

Normal blood pressure is important for proper blood flow to the body’s organs and tissues. The force of the blood on the walls of the arteries is called blood pressure. Blood pressure is measured both as the heart ...

344

Development and examination of blood substitute media for application in simulated blood circulation  

E-print Network

IAbstract Development and examination of blood substitute media for application in simulated blood and evaluates the applicability of potentially possible blood substitutes and their influence parameters behaviour of bio-degradable polymers. Furthermore, information about used blood substitutes, influencing

Berlin,Technische Universität

345

Evaluation of growth rate, body weight, heart rate, and blood parameters as potential indicators for selection against susceptibility to the ascites syndrome in young broilers.  

PubMed

The continuous selection for rapid growth has been accompanied by an increasing occurrence of ascites syndrome (AS), which develops in broilers failing to supply the increasing demand for O(2) in their bodies. Moderate heritability has been reported for AS in broiler populations, suggesting that selection against AS is feasible. However, direct selection based on AS mortality requires exposure of candidate birds to AS-inducing conditions (AIC), which hinder selection for performance traits. Noninvasive indicators of AS, expressed under standard husbandry, may facilitate the integration of selection against AS into breeding programs. This study was designed to look for differences in heart rate, hematocrit, O(2) saturation of hemoglobin in arterial blood (SaO(2)), BW, and weight gain, all measured at early ages under standard brooding conditions, between birds that later developed AS and those that remained healthy under AIC, and to estimate the heritability of these AS-related parameters and their genetic correlation with the tendency of broilers to develop AS. The experimental population was derived from a broiler dam line. Male progeny of 34 half-sib sire families were reared under standard brooding conditions to 19 d of age, then under an AIC protocol consisting of housing in individual cages, cool air high-speed ventilation, and growth enhancement using high-energy pelleted feed and 23 h/d of light. Birds were necropsied upon mortality or at the end of the trials and were recorded as being susceptible, with manifestations of AS (SUS), or resistant and healthy (RES). About 44% developed AS, confirming the efficacy of the novel AIC protocol. The SUS and RES chicks did not differ in BW and weight gain up to 19 d of age, suggesting that there was no association between AS susceptibility and rapid early growth. The SUS chicks exhibited lower SaO(2) and heart rate than the RES chicks. Moderate heritability was estimated for all traits, but only SaO(2) exhibited consistently significant genetic correlation (-0.5) with AS, suggesting that it may serve as an early indicator for selection against AS, albeit with a limited efficacy. PMID:17369531

Druyan, S; Shlosberg, A; Cahaner, A

2007-04-01

346

Plasma volume and biochemical changes in athletes during bed rest chronic hyperhydration  

NASA Astrophysics Data System (ADS)

Daily fluid and salt supplements (FSS) may be used to reduce plasma biochemical changes during bed rest (BR). The aim of this study was to evaluate the effect of a daily intake of FSS on plasma volume (PV) and biochemical changes during BR. Studies were done during a pre BR period of 15 days and during a BR period of 30 days. Thirty male athletes aged 22-26 years were chosen as subjects. They were divided into three groups: unsupplemented ambulatory control subjects (UACS), unsupplemented bed rested subjects (UBRS) and supplemented bed rested subjects (SBRS). The UBRS and SBRS were kept under a rigorous bed rest regime for 30 days. The SBRS took 26 ml water/kg body weight and 0.1 g sodium chloride/kg body weight daily. PV, protein, albumin, sodium (Na), Chloride (Cl), potassium (K), osmolality, creatinine, glucose, and whole blood haematocrit (Hct) and haemoglobin (Hb) concentrations were measured. PV increased significantly ( P?0.01) while plasma protein, albumin, Na, Cl, K, glucose, creatinine, osmolality, and whole blood Hb and Hct concentration decreased significantly ( P?0.01) in the SBRS group when compared with the UBRS group. By contrast, PV decreased significantly ( P?0.01), while plasma protein, albumin, Na, Cl, K, glucose, creatinine, osmolality and whole blood Hct and Hb concentration increased significantly ( P?0.01) in the UBRS group when compared with the SBRS and UACS groups. The measured parameters did not change significantly in the UACS group when compared with the baseline control values. It was concluded that a daily intake of FSS may be used to attenuate PV losses and biochemical changes in endurance trained athletes during bed rest.

Zorbas, Yan G.; Yarullin, Vladimir L.; Denogradov, Sergei D.; Afonin, Victor B.

347

Safety evaluation of allogeneic umbilical cord blood mononuclear cell therapy for degenerative conditions  

PubMed Central

Background The current paradigm for cord blood transplantation is that HLA matching and immune suppression are strictly required to prevent graft versus host disease (GVHD). Immunological arguments and historical examples have been made that the use of cord blood for non-hematopoietic activities such as growth factor production, stimulation of angiogenesis, and immune modulation may not require matching or immune suppression. Methods 114 patients suffering from non-hematopoietic degenerative conditions were treated with non-matched, allogeneic cord blood. Doses of 1-3 × 107 cord blood mononuclear cells per treatment, with 4-5 treatments both intrathecal and intravenously were performed. Adverse events and hematological, immunological, and biochemical parameters were analyzed for safety evaluation. Results No serious adverse effects were reported. Hematological, immunological, and biochemical parameters did not deviate from normal ranges as a result of therapy. Conclusion The current hematology-based paradigm of need for matching and immune suppression needs to be revisited when cord blood is used for non-hematopoietic regenerative purposes in immune competent recipients. PMID:20682053

2010-01-01

348

Is the formula of Traub still up to date in antemortem blood glucose level estimation?  

PubMed

According to the hypothesis of Traub, also known as the 'formula of Traub', postmortem values of glucose and lactate found in the cerebrospinal fluid or vitreous humor are considered indicators of antemortem blood glucose levels. However, because the lactate concentration increases in the vitreous and cerebrospinal fluid after death, some authors postulated that using the sum value to estimate antemortem blood glucose levels could lead to an overestimation of the cases of glucose metabolic disorders with fatal outcomes, such as diabetic ketoacidosis. The aim of our study, performed on 470 consecutive forensic cases, was to ascertain the advantages of the sum value to estimate antemortem blood glucose concentrations and, consequently, to rule out fatal diabetic ketoacidosis as the cause of death. Other biochemical parameters, such as blood 3-beta-hydroxybutyrate, acetoacetate, acetone, glycated haemoglobin and urine glucose levels, were also determined. In addition, postmortem native CT scan, autopsy, histology, neuropathology and toxicology were performed to confirm diabetic ketoacidosis as the cause of death. According to our results, the sum value does not add any further information for the estimation of antemortem blood glucose concentration. The vitreous glucose concentration appears to be the most reliable marker to estimate antemortem hyperglycaemia and, along with the determination of other biochemical markers (such as blood acetone and 3-beta-hydroxybutyrate, urine glucose and glycated haemoglobin), to confirm diabetic ketoacidosis as the cause of death. PMID:22327538

Palmiere, Cristian; Sporkert, Frank; Vaucher, Paul; Werner, Dominique; Bardy, Daniel; Rey, François; Lardi, Christelle; Brunel, Christophe; Augsburger, Marc; Mangin, Patrice

2012-05-01

349

Spatial heterogeneity of blood flow in the dog heart. I. Glucose uptake, free adenosine and oxidative\\/glycolytic enzyme activity  

Microsoft Academic Search

The spatial heterogeneity of myocardial perfusion and metabolism was studied in 11 anaesthetized dogs under resting conditions.\\u000a In each heart local myocardial blood flow was assessed using the tracer microsphere technique in 256 samples (mean mass: 83.1\\u000a mg) taken from the left anterior ventricular wall. In the same samples, the following biochemical parameters were determined:\\u000a accumulation of [3H]-deoxyglucose (a measure

Andreas Deussen; Jan Schultz; Robert Loncar; Jürgen Schrader; Waldemar Hort

1996-01-01

350

Autologous cord blood transfusion.  

PubMed

Newborn piglets were exsanguinated (60% of blood volume) and retransfused 1 h later. One test group received adult pig red blood cells, the other piglet cord blood cells; controls were infused with plasma. While all controls died, satisfactory results were achieved in piglets transfused with either adult or foetal blood. The feasibility of collecting human cord blood for transfusion was assessed in 100 samples of human cord blood. Blood was collected aseptically and aerobic and anaerobic cultures set up. All samples of cord blood were sterile, and all were Mycoplasma negative. Coagulation parameters were analysed in eight cord plasma samples stored at -20 degrees C for 45 days. No significant abnormalities were found immediately after birth or after storage. PMID:7949798

Ballin, A; Kenet, G; Gutman, R; Samra, Z; Zakut, H; Meytes, D

1994-07-01

351

Effect of pravastatin on echocardiographic circulation parameters in dogs.  

PubMed

The purpose of this study was to determine the effect of pravastatin (PS) on hemodynamic parameters in healthy dogs. Five beagle dogs were repeatedly used in each of the 4 groups. One group was not medicated (control). Dogs in other groups received 0.5, 1.0 or 2.0 mg/kg PS orally q24hr, for 4 weeks. Physical examination, blood biochemical tests, blood pressure measurements and Doppler echocardiography were performed before and 1, 2 and 4 weeks after PS administration in all dogs. PS significantly reduced the left atrial-to-aortic diameter ratio (LA/Ao), early diastolic transmitral flow (E) wave, E/early diastolic mitral annulus motion velocity (Em) ratio, left ventricular (LV) fractional shortening, LV ejection fraction, mid systolic myocardial velocity gradient, stroke volume (SV), cardiac output (CO), right and left ventricular Tei indices and elevated Em and early diastolic myocardial velocity gradient. Heart rate was not significantly altered during PS administration, but mean blood pressure decreased slightly. The hematological and blood biochemical values were within normal limits during PS administration. These results revealed that PS administration increases LV expansion capacity and decreases LV constriction and left atrial pressure. It has been suggested that PS may be effective in improving heart failures with LV diastolic dysfunction or elevated left atrial pressure in dogs. PMID:24317157

Arita, Shinji; Arita, Noboru; Hikasa, Yoshiaki

2014-04-01

352

Modulation of biochemical indices in common carp (Cyprinus carpio L.) under the influence of toxic cyanobacterial biomass in diet.  

PubMed

Cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. In the present study, we investigated the impact of cyanobacterial biomass on biochemical indices of common carp (Cyprinus carpio L., average weight of 246 ± 73 g) under laboratory conditions. The fish were fed a diet containing cyanobacterial biomass with microcystins in high concentration (0.4 mg/kg of fish weight and day) for 28 days. Statistical evaluation of the influence of the cyanobacterial biomass in food on the biochemical indices of the juvenile carp showed only minor differences. The activity of aspartate aminotransferase value and the urea concentration were significantly reduced compared to control group. The biochemical parameters of fish blood plasma significantly rose during the experiment in the control group as well as in the experimental group. This state was probably influenced by the environmental conditions and the fish diet. A significant rising value was established in calcium creatinine, total protein, phosphorus, lactate, urea and natrium. The present study demonstrates that the oral exposure of toxic cyanobacterial biomass has a minor influence on the biochemical indices of common carp and that the effect of other factors, e.g., nutrition is more visible. PMID:24972534

Kopp, Radovan; Palíková, Miroslava; Navrátil, Stanislav; Mareš, Jan

2014-12-01

353

Blood clotting  

MedlinePLUS Videos and Cool Tools

... the external bleeding stops. Clotting factors in the blood cause strands of blood-borne material, called fibrin, to stick together and ... the inside of the wound. Eventually, the cut blood vessel heals, and the blood clot dissolves after ...

354

Blood Transfusions  

MedlinePLUS

... United States get blood transfusions. A Bit About Blood Blood is like the body's transportation system, busy ... his or her body. Continue What Is a Blood Transfusion? A transfusion is a relatively simple medical ...

355

Blood Transfusion  

MedlinePLUS

... from the NHLBI on Twitter. What Is a Blood Transfusion? A blood transfusion is a safe, common ... Very rarely, serious problems develop. Important Information About Blood The heart pumps blood through a network of ...

356

Blood Thinners  

MedlinePLUS

If you have some kinds of heart or blood vessel disease, or if you have poor blood flow to your brain, your doctor may recommend that you take a blood thinner. Blood thinners reduce the risk of heart ...

357

Biochemical upgrading of oils  

DOEpatents

A process for biochemical conversion of heavy crude oils is provided. The process includes contacting heavy crude oils with adapted biocatalysts. The resulting upgraded oil shows, a relative increase in saturated hydrocarbons, emulsions and oxygenates and a decrease in compounds containing organic sulfur, organic nitrogen and trace metals. Adapted microorganisms which have been modified under challenged growth processes are also disclosed. 121 figs.

Premuzic, E.T.; Lin, M.S.

1999-01-12

358

Measures of Biochemical Sociology  

ERIC Educational Resources Information Center

In a previous article, the authors introduced a new sub field in sociology that we labeled "biochemical sociology." We introduced the definition of a sociology that encompasses sociological measures, psychological measures, and biological indicators Snell & Marsh (2003). In this article, we want to demonstrate a research strategy that would assess…

Snell, Joel; Marsh, Mitchell

2008-01-01

359

Biochemical upgrading of oils  

DOEpatents

A process for biochemical conversion of heavy crude oils is provided. The process includes contacting heavy crude oils with adapted biocatalysts. The resulting upgraded oil shows, a relative increase in saturated hydrocarbons, emulsions and oxygenates and a decrease in compounds containing in organic sulfur, organic nitrogen and trace metals. Adapted microorganisms which have been modified under challenged growth processes are also disclosed.

Premuzic, Eugene T. (East Moriches, NY); Lin, Mow S. (Rocky Point, NY)

1999-01-12

360

Probabilistic sensitivity analysis of biochemical reaction systems  

NASA Astrophysics Data System (ADS)

Sensitivity analysis is an indispensable tool for studying the robustness and fragility properties of biochemical reaction systems as well as for designing optimal approaches for selective perturbation and intervention. Deterministic sensitivity analysis techniques, using derivatives of the system response, have been extensively used in the literature. However, these techniques suffer from several drawbacks, which must be carefully considered before using them in problems of systems biology. We develop here a probabilistic approach to sensitivity analysis of biochemical reaction systems. The proposed technique employs a biophysically derived model for parameter fluctuations and, by using a recently suggested variance-based approach to sensitivity analysis [Saltelli et al., Chem. Rev. (Washington, D.C.) 105, 2811 (2005)], it leads to a powerful sensitivity analysis methodology for biochemical reaction systems. The approach presented in this paper addresses many problems associated with derivative-based sensitivity analysis techniques. Most importantly, it produces thermodynamically consistent sensitivity analysis results, can easily accommodate appreciable parameter variations, and allows for systematic investigation of high-order interaction effects. By employing a computational model of the mitogen-activated protein kinase signaling cascade, we demonstrate that our approach is well suited for sensitivity analysis of biochemical reaction systems and can produce a wealth of information about the sensitivity properties of such systems. The price to be paid, however, is a substantial increase in computational complexity over derivative-based techniques, which must be effectively addressed in order to make the proposed approach to sensitivity analysis more practical.

Zhang, Hong-Xuan; Dempsey, William P.; Goutsias, John

2009-09-01

361

Chemical Immobilization of Sloth Bears (Melursus ursinus) with Ketamine Hydrochloride and Xylazine Hydrochloride: Hematology and Serum Biochemical Values  

PubMed Central

The present study was conducted to define the physiological responses of captive sloth bears immobilized with ketamine hydrochloride and xylazine hydrochloride and to determine and compare the values of hematology and serum biochemical parameters between sexes. A total of 15 sloth bears were immobilized using combination of ketamine hydrochloride and xylazine hydrochloride drugs at the dose rate of 5.0?milligram (mg) per kg body weight and 2.0?mg per kg body weight, respectively. The use of combination of these drugs was found satisfactory for the chemical immobilization of captive sloth bears. There were no significant differences observed in induction time and recovery time and physiological parameters such as heart rate, respiratory rate, and rectal temperature between sexes. Health related parameters comprising hematological values like packed cell volume (PCV), hemoglobin (Hb), red blood cell count (RBC), erythrocyte indices, and so forth and biochemical values like total protein, blood urea nitrogen (BUN), creatinine, alkaline amino-transferase (ALT), aspartate amino-transferase (AST), and so forth were estimated in 11 (5 males and 6 females) apparently healthy bears. Comparison between sexes revealed significant difference in PCV (P < 0.05) and mean corpuscular hemoglobin concentration (MCHC) (P < 0.05). The study might help to evaluate health profiles of sloth bears for appropriate line treatment. PMID:24876990

Veeraselvam, M.; Sridhar, R.; Perumal, P.; Jayathangaraj, M. G.

2014-01-01

362

Chemical Immobilization of Sloth Bears (Melursus ursinus) with Ketamine Hydrochloride and Xylazine Hydrochloride: Hematology and Serum Biochemical Values.  

PubMed

The present study was conducted to define the physiological responses of captive sloth bears immobilized with ketamine hydrochloride and xylazine hydrochloride and to determine and compare the values of hematology and serum biochemical parameters between sexes. A total of 15 sloth bears were immobilized using combination of ketamine hydrochloride and xylazine hydrochloride drugs at the dose rate of 5.0?milligram (mg) per kg body weight and 2.0?mg per kg body weight, respectively. The use of combination of these drugs was found satisfactory for the chemical immobilization of captive sloth bears. There were no significant differences observed in induction time and recovery time and physiological parameters such as heart rate, respiratory rate, and rectal temperature between sexes. Health related parameters comprising hematological values like packed cell volume (PCV), hemoglobin (Hb), red blood cell count (RBC), erythrocyte indices, and so forth and biochemical values like total protein, blood urea nitrogen (BUN), creatinine, alkaline amino-transferase (ALT), aspartate amino-transferase (AST), and so forth were estimated in 11 (5 males and 6 females) apparently healthy bears. Comparison between sexes revealed significant difference in PCV (P < 0.05) and mean corpuscular hemoglobin concentration (MCHC) (P < 0.05). The study might help to evaluate health profiles of sloth bears for appropriate line treatment. PMID:24876990

Veeraselvam, M; Sridhar, R; Perumal, P; Jayathangaraj, M G

2014-01-01

363

Blood Gases, Biochemistry, and Hematology of Galapagos Green Turtles (Chelonia Mydas)  

PubMed Central

The green turtle, Chelonia mydas, is an endangered marine chelonian with a circum-global distribution. Reference blood parameter intervals have been published for some chelonian species, but baseline hematology, biochemical, and blood gas values are lacking from the Galapagos sea turtles. Analyses were done on blood samples drawn from 28 green turtles captured in two foraging locations on San Cristóbal Island (14 from each site). Of these turtles, 20 were immature and of unknown sex; the other eight were males (five mature, three immature). A portable blood analyzer (iSTAT) was used to obtain near immediate field results for pH, lactate, pO2, pCO2, HCO3?, Hct, Hb, Na, K, iCa, and Glu. Parameter values affected by temperature were corrected in two ways: (1) with standard formulas; and (2) with auto-corrections made by the iSTAT. The two methods yielded clinically equivalent results. Standard laboratory hematology techniques were employed for the red and white blood cell counts and the hematocrit determination, which was also compared to the hematocrit values generated by the iSTAT. Of all blood analytes, only lactate concentrations were positively correlated with body size. All other values showed no significant difference between the two sample locations nor were they correlated with body size or internal temperature. For hematocrit count, the iSTAT blood analyzer yielded results indistinguishable from those obtained with high-speed centrifugation. The values reported in this study provide baseline data that may be useful in comparisons among populations and in detecting changes in health status among Galapagos sea turtles. The findings might also be helpful in future efforts to demonstrate associations between specific biochemical parameters and disease. PMID:24824065

Lewbart, Gregory A.; Hirschfeld, Maximilian; Denkinger, Judith; Vasco, Karla; Guevara, Nataly; García, Juan; Muñoz, Juanpablo; Lohmann, Kenneth J.

2014-01-01

364

Effects of different fibrinogen concentrations on blood loss and coagulation parameters in a pig model of coagulopathy with blunt liver injury  

Microsoft Academic Search

INTRODUCTION: The early application of fibrinogen could potentially reverse haemodilution-induced coagulopathy, although the impact of varying concentrations of fibrinogen to reverse dilutional coagulopathy has not been studied in vivo. We postulated that fibrinogen concentration is correlated with blood loss in a pig model of coagulopathy with blunt liver injury. METHODS: Coagulopathy was induced in 18 anaesthetized pigs (32 ± 1.6

Oliver Grottke; Till Braunschweig; Dietrich Henzler; Mark Coburn; Rene Tolba; Rolf Rossaint

2010-01-01

365

Measurement of nicotine intake in pregnant women--associations to changes in blood cell count.  

PubMed

Self-reported information about smoking is imprecise and subject to bias, with accuracy varying according to circumstances. Biochemical assessment gives much clearer indications of the effects of tobacco intake on physiological parameters. As part of a randomized controlled trial, a new point-of-care test for smoking was used as a tool to reduce smoking in pregnancy. Measurements of nicotine metabolites in urine were related to a physiological effect of smoking, notably changes to blood parameters, assessed as a routine part of antenatal care. One hundred and eighty-seven pregnant women attending outpatient antenatal care were initially questioned by a midwife about smoking habits and later questioned by the investigators, during which the test was performed and the results relayed back to the patient. Self-reported smoking habit and cigarette consumption, either reported to midwives or the investigators, were shown to be poor indicators of the effects of smoking on blood parameters. The biochemical assessment of nicotine intake was significantly related to white blood count, haemoglobin concentration, haematocrit, mean cell volume, and mean cell haemoglobin. Red cell count, mean cell haemoglobin concentration, and platelet count were unrelated to nicotine metabolite measurements. We concluded that the new test was a reliable measure of nicotine intake in pregnancy and the results correlated with smoking-related changes to haematological parameters. PMID:11403725

Cope, G F; Nayyar, P; Holder, R

2001-05-01

366

Blood Disorders  

MedlinePLUS

... called plasma, is made of water, salts and protein. Over half of your blood is plasma. The solid part of your blood contains red blood cells, white blood cells and platelets. Blood disorders affect one or more parts of the blood and ...

367

Blood sugar test - blood  

MedlinePLUS

... between 70 and 100 milligrams per deciliter (mg/dL) is considered normal. If you had a random ... blood glucose level will be below 125 mg/dL. The examples above show the common measurements for ...

368

Biochemical Effects of Energy Drinks Alone or in Combination with Alcohol in Normal Albino Rats  

PubMed Central

Purpose: To determine the biochemical effects of energy drink alone or in combination with alcohol in normal albino rats. Methods: Twenty male albino rats weighing 160-180g were assigned into groups A-E of four rats per group. Group A and B rats were given low and high doses of ED, respectively, groups C and D were administered low and high doses of EDmA, respectively while group E rats were given distilled water and served as control. The treatment lasted for 30 days after which the animals were killed and their blood collected for laboratory analyses using standard methods. Results: There were no significant differences in body weight, packed cell volume and haemoglobin concentration with either administration of ED or EDmA in comparison to the control. Energy drink alone or EDmA has significant effects on total white blood cell count, plasma potassium, calcium, renal functions, liver enzymes and plasma triglycerides, with EDmA having more effects than ED alone, except for body weight where the energy drink alone has higher effect. Conclusion: Consumption of energy drink alone or in combination with alcohol is associated with significant alterations in some biochemical parameters. Caution should be exercised while consuming either of them. Public health education is urgently needed to correct the wrong impression already formed by the unsuspecting consumers, especially the youths. PMID:24409412

2014-01-01

369

Biochemical characterization of microcystin toxicity in rainbow trout ( Oncorhynchus mykiss)  

Microsoft Academic Search

The kinetics and biochemical effects of microcystins in rainbow trout were studied with freeze-dried toxic cells of Microcystis aeruginosa, strain PCC 7806. Following in vivo exposure the changes in liver histology were observed over a 72 hr period and the absorption of microcystins from the gastrointestinal tract into the blood and liver, as well as the inhibition of hepatic protein

Francesca Tencalla; Daniel Dietrich

1997-01-01

370

Morphological and biochemical changes after intravenous injection of gold nanoparticles  

Microsoft Academic Search

Advances in nanotechnology applications in medicine, including enhanced cancer therapy cause necessity investigation of nanoparticles toxicity. Herein, we report results encompassing the histological examination of tissues and biochemical tests of blood plasma after intravenous injection of gold nanoparticles. Besides of this, we analyzed passive accumulation of nanoshells in the tumor.

G. S. Terentyuk; G. N. Maslyakova; L. V. Suleymanova; V. B. Borodulin; Yu. S. Dudakova; N. G. Khlebtsov; B. N. Khlebtsov; G. G. Akchurin; I. L. Maksimova; V. V. Tuchin

2008-01-01

371

[About the biochemical criteria of heroin (narcotic) intoxication].  

PubMed

The article deals with the data of study of biochemical indicators and activity of particular proteolytic enzymes in blood serum of patients with heroin drug addiction. The results can be applied to detect the typical laboratory changes intrinsic to this kind of intoxication. PMID:24340942

Korshunov, G V; BYchkov, E N; Borodulin, V B; Arsent'eva, L A; Serkova, S A; Bel'skaia, N A

2013-06-01

372

Pituitary-adrenocortical axis, serum serotonin and biochemical response after halothane or isoflurane anaesthesia in rabbits.  

PubMed

To document the changes in serum serotonin, adrenocorticotrophic hormone (ACTH), corticosterone levels and select biochemical parameters in response to inhalant anaesthesia, 20 New Zealand White (NZW) rabbits were assigned to two treatment groups: halothane and isoflurane. Induction of anaesthesia was achieved using a face mask (3.5% halothane and 4.5% isoflurane in oxygen) followed by endotracheal intubation and maintenance of anaesthesia for 30 min (1.5% halothane and 2.5% isoflurane in oxygen). Blood samples were obtained before anaesthetic induction, and at 1, 10, 30, 60, 120 min and 24, 48 and 72 h after endotracheal intubation. Serum serotonin and corticosterone levels were measured by competitive enzyme immunoassay, ACTH by radioimmunoassay. Serum glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN) and creatinine levels were measured using an automated analyser. Significant increases in serum ACTH and corticosterone levels occurred after halothane administration while serum serotonin levels did not change. An increase in serum corticosterone and serotonin levels occurred in the isoflurane group but no changes in ACTH concentrations were detected. Administration of halothane significantly increased serum glucose, ALT, AST, BUN and creatinine levels. After isoflurane administration, there was a significant increase in serum glucose, AST, BUN and creatinine levels. Based on these results, halothane stimulates the hypothalamic-pituitary-adrenal axis to a greater extent than isoflurane, but isoflurane increases serum serotonin levels. Both anaesthetic agents alter select biochemical parameters. These results should be taken into account when blood samples are evaluated in treated isoflurane or halothane anaesthetized rabbits. PMID:17988436

Gil, Alfredo González; Silván, Gema; Illera, Juan C

2007-10-01

373

Optical assay of erythrocyte function in banked blood.  

PubMed

Stored red blood cells undergo numerous biochemical, structural, and functional changes, commonly referred to as storage lesion. How much these changes impede the ability of erythrocytes to perform their function and, as result, impact clinical outcomes in transfusion patients is unknown. In this study we investigate the effect of the storage on the erythrocyte membrane deformability and morphology. Using optical interferometry we imaged red blood cell (RBC) topography with nanometer sensitivity. Our time-lapse imaging quantifies membrane fluctuations at the nanometer scale, which in turn report on cell stiffness. This property directly impacts the cell's ability to transport oxygen in microvasculature. Interestingly, we found that cells which apparently maintain their normal shape (discocyte) throughout the storage period, stiffen progressively with storage time. By contrast, static parameters, such as mean cell hemoglobin content and morphology do not change during the same period. We propose that our method can be used as an effective assay for monitoring erythrocyte functionality during storage time. PMID:25189281

Bhaduri, Basanta; Kandel, Mikhail; Brugnara, Carlo; Tangella, Krishna; Popescu, Gabriel

2014-01-01

374

Biochemical assessment of acute myocardial ischaemia.  

PubMed Central

AIMS--To evaluate the efficacy of biochemical parameters in different fluids in the diagnosis of myocardial infarction of different causes, analysed after death. METHODS--The myoglobin concentration and total creatine kinase (CK) and creatine kinase MB isoenzyme (CK-MB) activities were measured in serum, pericardial fluid, and vitreous humour from seven diagnostic groups of cadavers classified according to the severity of myocardial ischaemia and cause of death. Lactate dehydrogenase (LDH) and myosin were measured only in serum and pericardial fluid, and cathepsin D only in pericardial fluid. Routine haematoxylin and eosin and acridine orange staining were used for microscopy studies of heart tissue. RESULTS--In pericardial fluid there were substantial differences between the different groups with respect to CK, CK-MB, and LDH activities and myosin concentrations. The highest values were found in cases with morphological evidence of myocardial ischaemia. CONCLUSIONS--Biochemical parameters, which reach the pericardial fluid via passive diffusion and ultrafiltration due to a pressure gradient, were thus detectable in this fluid earlier than in serum in cases with myocardial ischaemia. These biochemical parameters may be of use for ruling out myocardial ischaemia in those controversial cases in which reliable morphological findings are lacking. PMID:7745110

Perez-Cárceles, M D; Osuna, E; Vieira, D N; Martínez, A; Luna, A

1995-01-01

375

Serum biochemical and haematological reference intervals for water buffalo Bubalus bubalis heifers.  

PubMed

Based on a review of the literature, reference intervals for water buffalo (Bubalus bubalis) serum biochemistry and haematology have not previously been published. The current study was done to establish reference intervals for water buffalo heifers. The International Federation of Clinical Chemistry stated that at least 120 values are necessary to obtain reliable estimates for reference intervals. A total number of 127 clinically healthy buffalo heifers (1-2 years old) were included in the study. Animals were examined at buffalo farms that belong to Assiut Governorate, Egypt. Three types of samples were collected: serum samples for biochemical analysis, whole blood samples for haematological analysis and faecal samples for parasitological examination. Animals that fitted the inclusion criteria were included in the study. Biochemical analysis included serum total proteins, albumin, total globulins, alpha, beta and gamma globulin levels, and aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, creatine phosphokinase and lactate dehydrogenase activity. In addition to the above, serum creatinine, urea, total bilirubin, direct bilirubin, indirect bilirubin, sodium, potassium, chloride, magnesium, calcium, phosphorus, copper, zinc, iron, triglycerides, high density lipoprotein, low density lipoprotein, very low density lipoprotein, glucose levels and 20 haematological variables were measured. The 95.0% reference intervals were calculated by removing the upper and lower 2.5% of the interval for each serum biochemical constituent to give the 2.5 and 97.5 percentiles. Confidence intervals were calculated for each reference limit. Reference intervals from the current study were compared with established values for cows. The current study is as far as could be determined the first that establishes reference intervals for the serum biochemical and haematological parameters in water buffalo heifers. PMID:24831856

Abd Ellah, Mahmoud R; Hamed, Maha I; Ibrahim, Derar R; Rateb, Hassan Z

2014-01-01

376

Hematological and biochemical alterations in the fish Prochilodus lineatus caused by the herbicide clomazone.  

PubMed

The indiscriminate use of herbicides has led to the contamination of water bodies, possibly affecting the health of aquatic biota. Therefore, to evaluate the possible effects of the clomazone-based herbicide (Gamit(®) 500) on the fish Prochilodus lineatus, juveniles were exposed for 96h to three concentrations (1, 5 and 10mgL(-1)) of clomazone, and an analysis was made of their hematological parameters: hemoglobin (Hb); hematocrit (Hct); red blood cell (RBC) count; mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); mean corpuscular hemoglobin concentration (MCHC) and biochemical parameters: glutathione S-transferase (GST); catalase (CAT); glutathione peroxidase (GPx) and acetylcholinesterase (AChE). Hct presented a significant decrease at the concentration of 10mgL(-1), while the parameters Hb, HCM and MCHC presented a significant decrease at the two higher concentrations, indicating an anemic condition. The RBC increased significantly at the lowest concentration, possibly due to the release of new red blood cells into the bloodstream in response to splenic contraction, which may occur as an adaptive response to the stressor agent. P. lineatus presented activation of the biotransformation pathway, indicated by augmented hepatic activity of the enzyme GST and hepatic activation of the antioxidant enzyme CAT at the higher concentrations. Liver GPx was significantly inhibited at the higher concentrations, which may indicate the efficient action of CAT in the elimination of H2O2 or its competition with GST for the same substrate (GSH). AChE activity in brain and muscle was inhibited at the higher concentrations, indicating the neurotoxic effects of the herbicide in the fish. The hematological and biochemical alterations led to the conclusion that the herbicide clomazone has toxic effects on the species P. lineatus, and that its presence in the environment may jeopardize the health of these animals. PMID:23538322

Pereira, Lindalva; Fernandes, Marisa N; Martinez, Cláudia B R

2013-07-01

377

Pesticide exposure and genetic variation in xenobiotic-metabolizing enzymes interact to induce biochemical liver damage.  

PubMed

Metabolic activation of pesticides in the liver may result in highly reactive intermediates capable of impairing various cellular functions. Nevertheless, the knowledge about the effect of pesticide exposure on liver function is still limited. This study assessed whether exposure to pesticides elicits early biochemical changes in biomarkers of liver function and looked for potential gene-environmental interactions between pesticide exposure and polymorphisms of pesticide-metabolizing genes. A longitudinal study was conducted in farm-workers from Andalusia (South Spain), during two periods of the same crop season with different degree of pesticide exposure. Blood samples were taken for the measurement of serum and erythrocyte cholinesterase activities as well as for determining clinical chemistry parameters as biomarkers of liver function. Serum lipid levels were also measured as they may help to monitor the progress of toxic liver damage. A reduction in serum cholinesterase was associated with decreased levels of all clinical chemistry parameters studied except HDL-cholesterol. Conversely, a decreased erythrocyte cholinesterase (indicating long-term pesticide exposure) was associated with increased levels of aspartate aminotransferase and alkaline phosphatase and increased levels of triglycerides, total cholesterol and LDL-cholesterol, but reduced levels of HDL-cholesterol. Changes in liver biomarkers were particularly associated with the PON155M/192R haplotype. The obtained results therefore support the hypothesis that pesticide exposure results in subtle biochemical liver toxicity and highlight the role of genetic polymorphisms in pesticide-metabolizing enzymes as biomarkers of susceptibility for developing adverse health effects. PMID:23688862

Hernández, Antonio F; Gil, Fernando; Lacasaña, Marina; Rodríguez-Barranco, Miguel; Tsatsakis, Aristidis M; Requena, Mar; Parrón, Tesifón; Alarcón, Raquel

2013-11-01

378

On Better Estimating and Normalizing the Relationship between Clinical Parameters: Comparing Respiratory Modulations in the Photoplethysmogram and Blood Pressure Signal (DPOP versus PPV)  

PubMed Central

DPOP (?POP or Delta-POP) is a noninvasive parameter which measures the strength of respiratory modulations present in the pulse oximeter waveform. It has been proposed as a noninvasive alternative to pulse pressure variation (PPV) used in the prediction of the response to volume expansion in hypovolemic patients. We considered a number of simple techniques for better determining the underlying relationship between the two parameters. It was shown numerically that baseline-induced signal errors were asymmetric in nature, which corresponded to observation, and we proposed a method which combines a least-median-of-squares estimator with the requirement that the relationship passes through the origin (the LMSO method). We further developed a method of normalization of the parameters through rescaling DPOP using the inverse gradient of the linear fitted relationship. We propose that this normalization method (LMSO-N) is applicable to the matching of a wide range of clinical parameters. It is also generally applicable to the self-normalizing of parameters whose behaviour may change slightly due to algorithmic improvements.

Addison, Paul S.; Wang, Rui; Uribe, Alberto A.; Bergese, Sergio D.

2015-01-01

379

Immunoelectrophoresis - blood  

MedlinePLUS

IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum immunoglobulin electrophoresis ... A blood sample is needed. For information on how this is done, see: Venipuncture

380

Urea, sugar, nonesterified fatty acid and cholesterol content of the blood in prolonged weightlessness  

NASA Technical Reports Server (NTRS)

Biochemical blood composition studies on astronauts during weightlessness flight simulation tests and during actual space flights showed some disturbances of metabolic processes. Increases in blood sugar, fatty acid and cholesterol, and urea content are noted.

Balakhovskiy, I. S.; Orlova, T. A.

1975-01-01

381

Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet--A radiation on African catfish Clarias gariepinus (Burchell, 1822).  

PubMed

The present study aimed to elucidate the negative impacts of UVA on some biochemical and hematological variables of the economically important African catfish, Clarias gariepinus and investigates the putative role of quince (Cydonia oblonga Miller) leaf extract in protection and/or alleviation of such negative impacts. Changes in the hematological and blood biochemical values often reflect alteration of physiological state. Blood parameters can be useful for the measurement of physiological disturbances in stressed fish and thus provide information about the level of damage in the fish. We found a significant (P<0.05) decrease in the red blood cell counts, hemoglobin and hematocrit in the groups exposed to UVA compared to the control groups. Exposure to UVA induced marked red cell shrinkage (increased mean cell hemoglobin concentration) and showed an elevation in mean cell volume and mean cell hemoglobin in the blood of the exposed fish compared to the control. A significant (P<0.05) reduction in the total white blood cells was recorded in the exposed fish compared to the control. The biochemical parameters (blood glucose, total plasma protein, blood cholesterol, plasma creatinine, aspartic amino transferase and alanine amino transferase) exhibited a significant increase in the blood of fish exposed to UVA. Methanolic extract of quince leaf before ripening of the fruits was analyzed by GC/MS. To investigate the biological impact of this extract and its biologically active components, this extract was tested for its putative role in alleviation of UVA effect on catfish. Quince leaf extract had the ability to prevent hematotoxic stress induced by UVA and resulted in enhancement of the immune system of catfish represented by significant (P<0.05) increase in the number of white blood cells and lymphocytes of the catfish. Quince extract also protected the red blood cells from UVA damage. To our knowledge this is the first report of the effect of quince leaf extract on an aquatic organism. PMID:20206545

Osman, Alaa G M; Koutb, Mostafa; Sayed, Alaa El-Din H

2010-04-01

382

Causal network inference using biochemical kinetics  

PubMed Central

Motivation: Networks are widely used as structural summaries of biochemical systems. Statistical estimation of networks is usually based on linear or discrete models. However, the dynamics of biochemical systems are generally non-linear, suggesting that suitable non-linear formulations may offer gains with respect to causal network inference and aid in associated prediction problems. Results: We present a general framework for network inference and dynamical prediction using time course data that is rooted in non-linear biochemical kinetics. This is achieved by considering a dynamical system based on a chemical reaction graph with associated kinetic parameters. Both the graph and kinetic parameters are treated as unknown; inference is carried out within a Bayesian framework. This allows prediction of dynamical behavior even when the underlying reaction graph itself is unknown or uncertain. Results, based on (i) data simulated from a mechanistic model of mitogen-activated protein kinase signaling and (ii) phosphoproteomic data from cancer cell lines, demonstrate that non-linear formulations can yield gains in causal network inference and permit dynamical prediction and uncertainty quantification in the challenging setting where the reaction graph is unknown. Availability and implementation: MATLAB R2014a software is available to download from warwick.ac.uk/chrisoates. Contact: c.oates@warwick.ac.uk or sach@mrc-bsu.cam.ac.uk Supplementary information: Supplementary data are available at Bioinformatics online. PMID:25161235

Oates, Chris J.; Dondelinger, Frank; Bayani, Nora; Korkola, James; Gray, Joe W.; Mukherjee, Sach

2014-01-01

383

Prevention of cytogenetic, histochemical and biochemical alterations in Oreochromis niloticus by dietary supplement of sorbent materials.  

PubMed

The current study was conducted to evaluate the ability of Egyptian bentonite (EB) and montmorillonite (EM) for the prevention of genotoxicity, histochemical and biochemical changes induced by aflatoxin B(1) (AFB(1)) using the micronucleus (MN) assay, chromosomal aberrations and DNA fragmentation analysis in Tilapia fish. Six groups of fish were treated for 3 weeks and included the control group, AFB(1)-treated group and the groups treated with EB or EM alone or in combination with AFB(1). At the end of experiment period, blood samples were collected for MN, testosterone and biochemical assays. Chromosomal aberrations were determined in kidney tissues, DNA fragmentation test was determined in liver and testis, whereas histochemical study was carried out on liver, testis and gills. The results indicated that a significant decrease in total protein, albumin, globulin, testosterone and DNA content in liver, gills and testis accompanied with a significant increase in number of micronucleated erythrocytes (MnRBCs), total chromosomal aberrations in kidney and DNA fragmentation in testis and liver of fish received AFB(1) alone. Fish treated with EB or EM alone were comparable to the control regarding the biochemical parameters except testosterone in EB-treated group which was significantly decreased. Both clays did not induce any significant differences in number of MnRBCs, chromosomal aberrations in the kidney, DNA fragmentation in testis, but not in liver of EB-treated group. The combined treatment with AFB(1) and EB or EM succeeded to improve all the tested parameters towards the control values although it did not normalize them. Moreover, the improvement was pronounced in the group received EM plus AFB(1). It could be concluded that EB and EM have the ability to tightly bind AFB(1) in the gastrointestinal tract of fish resulting in decreasing its bioavailability. Moreover, the two tested clays were safe and can be used as potential aflatoxin binders in animal feed. PMID:20817254

Hassan, Aziza M; Kenawy, Amany M; Abbas, Wafaa T; Abdel-Wahhab, Mossad A

2010-11-01

384

Biochemical and hematological studies on perch, Perca fluviatilis, from the cadmium-contaminated river Eman  

SciTech Connect

In a field investigation, biochemical and hematological parameters were measured in perch, Perca fluviatilis, living in the cadmium-contaminated river Eman in the southeast of Sweden. The number of lymphocytes was 45-100% higher in perch from the contaminated area than in the reference perch, indicating a stimulated immune defense. In addition, the cadmium-loaded fish suffered from a slight anemia and a disturbed carbohydrate metabolism and blood plasma ion composition. The observed effects suggest that cadmium affects fish in the environment similar to what has been observed in laboratory studies. However, the weaker response may imply that the perch in the field stimulation have been adapted and thus acquired an increased resistance to cadmium.

Sjoebeck, M.L.; Haux, C.; Larsson, A.; Lithner, G.

1984-06-01

385

Biochemical effects of long-term exposure to cadmium and copper on rainbow trout (Salmo gairdneri): validation of water quality criteria  

SciTech Connect

Salmo gairdneri specimens were exposed for 4 months to 1-10 micrograms/liter of Cd and 30-100 micrograms/liter of Cu. At different times from the beginning of the experiment the activity of some metal-sensitive enzymes was assayed (blood catalase and carbonic anhydrase; liver aminolevulinate dehydrase and total leucylaminopeptidase ). Biochemical indices of mitochondrial (respiratory control ratio; O/sub 2/ consumption) and lysosomal function (percentage free leucylaminopeptidase activity), gill sialic acid content, and mucus lysozyme concentration were also detected. Cu (at both the levels tested) and Cd (only at the highest concentration) cause a remarkable alteration in various biochemical/physiological parameters under study. Considerations about the water quality criteria levels proposed by EIFAC /FAO for the two metals are reported.

Arillo, A.; Calamari, D.; Margiocco, C.; Melodia, F.; Mensi, P.

1984-04-01

386

ASSESSING WEIGHT-RELATED BIOCHEMICAL CARDIOVASCULAR RISK FACTORS IN AFRICAN-AMERICAN GIRLS.  

Technology Transfer Automated Retrieval System (TEKTRAN)

Hyperinsulinemia/insulin resistance is a risk factor for future type 2 diabetes. Fasting insulin and blood lipids serve as direct indicators of subsequent risk and as biochemical markers of metabolically significant adiposity. We examined the feasibility of obtaining fasting blood samples and report...

387

Influence of Nigella sativa fixed oil on some blood parameters and histopathology of skin in staphylococcal-infected BALB/c mice.  

PubMed

Nigella sativa has been used for a long time in Jordanian folk medicine to treat skin diseases like microbial infections and inflammation. Therefore, the present study was conducted to assess the healing efficacy of petroleum ether extract of Nigella sativa seeds (fixed oil) on staphylococcal-infected skin. Male BALB/c mice were infected with 100 microL of Staphylococcus aureus (ATCC 6538) suspension at a dose of 10(8) colony forming unit/mouse into shaved mild dorsal skin. Application of treatments for each group (100 microL sterile saline, 100 microL chloramphenicol (10 microg/mouse) and Nigella sativa fixed oil at a dose of 50, 100 or 150 microL/mouse) was performed at the site of infection twice a day for two consecutive days after 3 h of infection. At day 3 and 5 after infection, total White Blood Cells (WBCs) count; differential and absolute differential WBC counts and the number of viable bacteria present in the skin area were measured. At day 5 after infection, the animals were sacrificed and the histology of skin was examined. Results indicated that fixed oil of Nigella sativa seeds enhance healing of staphylococcal-infected skin by reducing total and absolute differential WBC counts, local infection and inflammation, bacterial expansion and tissue impairment. These effects provide scientific basis for the use of Nigella sativa in traditional medicine to treat skin infections and inflammations. PMID:22590837

Abu-Al-Basal, Mariam A

2011-12-01

388

Modeling the growth and dissolution of clots in flowing blood  

E-print Network

Multiple interacting mechanisms control the formation and dissolution of clots to maintain blood in a state of delicate balance. In addition to a myriad of biochemical reactions, rheological factors also play a crucial role in modulating...

Mohan, Anand

2006-10-30

389