Sample records for biochemical blood parameters

  1. Infrared spectra in monitoring biochemical parameters of human blood

    NASA Astrophysics Data System (ADS)

    Prabhakar, S.; Jain, N.; Singh, R. A.

    2012-05-01

    Infrared spectroscopy is gaining recognition as a promising method. The infrared spectra of selected regions (2000-400cm-1) of blood tissue samples are reported. Present study related to the role of spectral peak fitting in the study of human blood and quantitative interpretations of infrared spectra based on chemometrics. The spectral variations are interpreted in terms of the biochemical and pathological processes involved. The mean RNA/DNA ratio of fitted intensities and analytical area as calculated from the transmittance peaks at 1121cm-1/1020cm-1 is found to be 0.911A.U and 2.00A.U. respectively. The ratio of 1659cm-1/1544cm-1 (amide-I/amide-II) bands is found to shed light on the change in the DNA content. The ratio of amide-I/amide-II is almost unity (?1.054) for blood spectra. The deviation from unity is an indication of DNA absorption from the RBC cells. The total phosphate content has found to be 25.09A.U. The level for glycogen/phosphate ratio (areas under peaks 1030cm-1/1082cm-1) is found to be 0.286A.U. The ratio of unsaturated and saturated carbonyl compounds (C=O) in blood samples is in form of esters and the analytical areas under the spectral peaks at 1740cm-1 and 1731cm-1 for unsaturated esters and saturated esters respectively found to be 0.618A.U.

  2. Reference values for biochemical parameters in blood serum of young and adult alpacas (Vicugna pacos).

    PubMed

    Husakova, T; Pavlata, L; Pechova, A; Hauptmanova, K; Pitropovska, E; Tichy, L

    2014-09-01

    The aim of this study was to establish reference interval for biochemical parameters in blood of alpacas on the basis of large population of clinically healthy animals, and to determine the influence of sex, age and season on nitrogen and lipid metabolites, enzymes, electrolytes, vitamins and minerals in blood of alpacas. Blood samples were collected from 311 alpacas (61 males and 201 females >6 months of age and 49 crias (21 males and 28 females) ?6 months of age). Selected farms were located in Central Europe (Czech Republic and Germany). We determined 24 biochemical parameters from blood serum. We performed the comparison of results by the sex of animals and for the older group also the comparison of the results with regard to the season, respectively, to the feeding period. We found no highly significant difference (P<0.01) between males and females with the exception of ?-glutamyl transferase (GGT), alkaline phosphatase (ALP) and cholesterol. We found 15 significantly different parameters between the group of crias 6 months of age and the older alpacas. Based on our findings we suggest for most parameters to use different reference intervals (especially ALP, cholesterol, total protein, globulin, non-esterified fatty acids (NEFA), GGT and phosphorus) for the two above-mentioned age groups. Another important finding is the differences between some parameters in older group of alpacas in summer/winter feeding period. Animals in the summer feeding period have higher values of parameters related to fat mobilization (?-hydroxybutyrate, NEFA) and liver metabolism (bilirubin, alanine aminotransferase). The winter period with increased feeding of supplements with higher amount of fat, vitamins and minerals is characteristic by increased values of cholesterol, triglycerides, vitamins A and E, and some minerals (K, Ca, Mg and Cl) in blood serum. Clinical laboratory diagnosis of metabolic disturbances may be improved with use of age-based reference values and with consideration of seasonal differences. PMID:24852937

  3. The dynamics of blood biochemical parameters in cosmonauts during long-term space flights

    NASA Astrophysics Data System (ADS)

    Markin, Andrei; Strogonova, Lubov; Balashov, Oleg; Polyakov, Valery; Tigner, Timoty

    Most of the previously obtained data on cosmonauts' metabolic state concerned certain stages of the postflight period. In this connection, all conclusions, as to metabolism peculiarities during the space flight, were to a large extent probabilistic. The purpose of this work was study of metabolism characteristics in cosmonauts directly during long-term space flights. In the capillary blood samples taken from a finger, by "Reflotron IV" biochemical analyzer, "Boehringer Mannheim" GmbH, Germany, adapted to weightlessness environments, the activity of GOT, GPT, CK, gamma-GT, total and pancreatic amylase, as well as concentration of hemoglobin, glucose, total bilirubin, uric acid, urea, creatinine, total, HDL- and LDL cholesterol, triglycerides had been determined. HDL/LDL-cholesterol ratio also was computed. The crewmembers of 6 main missions to the "Mir" orbital station, a total of 17 cosmonauts, were examined. Biochemical tests were carryed out 30-60 days before lounch, and in the flights different stages between the 25-th and the 423-rd days of flights. In cosmonauts during space flight had been found tendency to increase, in compare with basal level, GOT, GPT, total amylase activity, glucose and total cholesterol concentration, and tendency to decrease of CK activity, hemoglobin, HDL-cholesterol concentration, and HDL/LDL — cholesterol ratio. Some definite trends in variations of other determined biochemical parameters had not been found. The same trends of mentioned biochemical parameters alterations observed in majority of tested cosmonauts, allows to suppose existence of connection between noted metabolic alterations with influence of space flight conditions upon cosmonaut's body. Variations of other studied blood biochemical parameters depends on, probably, pure individual causes.

  4. The effects of copper on blood and biochemical parameters of rainbow trout (Oncorhynchus mykiss)

    USGS Publications Warehouse

    Dethloff, G.M.; Schlenk, D.; Khan, S.; Bailey, H.C.

    1999-01-01

    Metals are released into aquatic systems from many sources, often at sublethal concentrations. The effects of sublethal concentrations of metals on fish are not entirely understood. The objective of this study was to determine the hematological and biochemical effects of a range of copper concentrations (6.4, 16.0, 26.9 ??g Cu/L) on rainbow trout (Oncorhynchus mykiss) over a prolonged period of time. Trout were exposed to copper, and, at intervals of 3, 7, 14, and 21 days, selected parameters were evaluated. Hemoglobin, hematocrit, plasma glucose, and plasma cortisol levels were elevated in trout exposed to 26.9 ??g Cu/L at day 3 and then returned to levels comparable to control fish. Plasma protein and lactate levels were not significantly altered in trout from any copper treatment. Hepatic copper concentration and hepatic metallothionein mRNA expression were consistently elevated in trout exposed to 26.9 ??g Cu/L. Both of these parameters stabilized by day 3, with only hepatic copper concentration showing a further increase at day 21. Hepatic copper concentration and hepatic metallothionein mRNA expression appear to be robust indicators of copper exposure. Most blood-based parameters evaluated appear to be associated with a transitory, nonspecific stress response. The return of elevated hematological and biochemical parameters to control levels after 3 days and thestabilization of hepatic metallothionein mRNA expression and copper concentration over a similar time period suggested acclimation to dissolved copper at 26.9 ??g/L. Further analysis of the data on blood-based parameters indicated that certain parameters (hemoglobin, hematocrit, plasma glucose, plasma cortisol) may be useful in field monitoring.

  5. The Effects of Ramadan Fasting and Physical Activity on Blood Hematological-Biochemical Parameters

    PubMed Central

    Attarzadeh Hosseini, Seyyed Reza; Hejazi, Keyvan

    2013-01-01

    Objective(s) : Fasting during the month of Ramadan is a religious obligation and belief for healthy adult Muslims. The aim of the present study was to determine the effects of Ramadan Fasting and physical activity on ‘Blood Hematological-Biochemical Parameters’. Materials and Methods: In this study, twenty-six healthy males in two experimental groups were compared in two different instances before and after the training period. The groups which were selected by convenience sampling method were divided into two non-active fasting (n=13) and active fasting (n=13) groups. For comparison purposes between groups, paired and independent sample t-test was performed, respectively, after ensuring their normality within a significance level of P?0.05. Results: HDL-C increased significantly in both active and non-active fasting groups, Moreover, amount of hematocrit (Hct), red blood cell count (RBC), TC, LDL, VLDL, LDL/HDL and TC/ HDL decreased significantly. Amount of hemoglobin (Hgb) and glucose reduced significantly in the active-fasting group. The variation of the means between the groups in the Hgb index and LDL/HDL were statistically significant. Conclusion: Fasting during the month of Ramadan by regular physical activity caused positive alterations in Hematological-Biochemical Index. These changes may be due to the alterations in diet, biology response of the body to the starving and physical activity during this month. PMID:23997915

  6. Effect of third generation ?-blockers on biochemical and rheological parameters of blood in rabbits stressed with electrical current

    Microsoft Academic Search

    Luisa Gabunia; Nino Tkhilava; Shalva Petriashvili; Ekaterina Ekaladze; Tamar Vardosanidze

    Have been studied effects of third generation ?-blockers - carvedilol and nebivalol on blood rheology and biochemical parameters in experimental rabbits on the background of electrical stress. Experiments have been carried out on adult rabbits (body weight 3-4 kg, n=22). They underwent stress after exposure to electric current. Presence and development of stress reaction was confirmed by the series of

  7. [Changes of biochemical blood parameters under conditions of treatment with vaulen and polysorb enterosorbents].

    PubMed

    Iaremenko, O B; Ter-Vartan'ian, S Kh; Lopushenko, L G; Mikhal'chuk, H A

    1994-01-01

    Alteration of the biochemical blood indices has been studied in patients with rheumatoid arthritis and lupus erythematosus that were treated with vaulen (V) and polysorb (P) enterosorbents. It was determined that alanine aminotransferase activity grew and albumin, urea, uric acid levels enhanced after the V treatment. Glucose level and alanine-, asparagine aminotransferases activities decreased after the P treatment. It is necessary to take into consideration these results when choosing an enterosorbent for treatment of patients with the immunocomplex rheumatic diseases accompanied by the liver parenchyma lesion and hyperuricemia. PMID:7754550

  8. Grape Seed Proanthocyanidin Extract Improved some of Biochemical Parameters and Antioxidant Disturbances of Red Blood Cells in Diabetic Rats

    PubMed Central

    Mansouri, Esrafil; Khorsandi, Layasadat; Zare Moaiedi, Maasoumeh

    2015-01-01

    Grape seed proanthocyanidin extract (GSPE) has a broad spectrum of biologic properties against oxidative stress. This study aimed to investigate the effects of GSPE on biochemical factors and antioxidant enzymes of erythrocyte in diabetic rats. Diabetes was induced through single injection of streptozotocin (50 mg.Kg-1, i.p). Forty Male Sprague-Dawley rats were divided into four Groups: Group 1, healthy control group; Group 2, healthy group treated with GSPE (200 mg.Kg-1); Group 3, diabetic control group; Group 4, diabetic group treated with GSPE (200 mg.Kg-1) for 4 weeks. At the end, the experimental animals were sacrificed and blood samples were collected and plasma parameters and erythrocytes antioxidant status were evaluated. The results show, treatment with GSPE significantly reduced (P<0.001) urine volume, proteinuria and biochemical factors such as blood urea nitrogen, creatinine, triglyceride, total cholesterol, low density lipoprotein and very low density lipoprotein as well as malondialdehyde. Also GSPE treatment significantly (P<0.005) increased high density lipoprotein, total protein and albumin. Moreover GSPE significantly increased antioxidant enzymes activity such as: superoxide dismutase, glutathione peroxidase and catalase. These results suggest that GSPE can ameliorate biochemical abnormalities and antioxidant system status in streptozotocin- induced diabetic rats probably by its potent antioxidant features. PMID:25561939

  9. Grape Seed Proanthocyanidin Extract Improved some of Biochemical Parameters and Antioxidant Disturbances of Red Blood Cells in Diabetic Rats.

    PubMed

    Mansouri, Esrafil; Khorsandi, Layasadat; Zare Moaiedi, Maasoumeh

    2015-01-01

    Grape seed proanthocyanidin extract (GSPE) has a broad spectrum of biologic properties against oxidative stress. This study aimed to investigate the effects of GSPE on biochemical factors and antioxidant enzymes of erythrocyte in diabetic rats. Diabetes was induced through single injection of streptozotocin (50 mg.Kg(-1), i.p). Forty Male Sprague-Dawley rats were divided into four Groups: Group 1, healthy control group; Group 2, healthy group treated with GSPE (200 mg.Kg(-1)); Group 3, diabetic control group; Group 4, diabetic group treated with GSPE (200 mg.Kg(-1)) for 4 weeks. At the end, the experimental animals were sacrificed and blood samples were collected and plasma parameters and erythrocytes antioxidant status were evaluated. The results show, treatment with GSPE significantly reduced (P<0.001) urine volume, proteinuria and biochemical factors such as blood urea nitrogen, creatinine, triglyceride, total cholesterol, low density lipoprotein and very low density lipoprotein as well as malondialdehyde. Also GSPE treatment significantly (P<0.005) increased high density lipoprotein, total protein and albumin. Moreover GSPE significantly increased antioxidant enzymes activity such as: superoxide dismutase, glutathione peroxidase and catalase. These results suggest that GSPE can ameliorate biochemical abnormalities and antioxidant system status in streptozotocin- induced diabetic rats probably by its potent antioxidant features. PMID:25561939

  10. Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii)

    USGS Publications Warehouse

    Feng, G.; Zhuang, P.; Zhang, L.; Kynard, B.; Shi, X.; Duan, M.; Liu, J.; Huang, X.

    2011-01-01

    The effects of MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii) were studied. MS-222 caused higher glucose (GLU) concentrations in anaesthetic test groups than for the control group. Triglyceride (TGL) concentrations of fish in the 140 and 160mgL-1 groups were also significantly higher than those of other groups. Alanine aminotransferase (ALT) activity in the 140mgL-1 group was significantly higher than the level in 80, 100 and 120mgL-1 groups. Aspartate aminotransferase (AST) activity in the 140mgL-1 group was significantly higher than those in the 100 and 120mgL-1 groups. Levels of total protein (TP), cholesterol (CHOL) and alkaline phosphatase (ALP) in anaesthetic test groups were not significantly influenced by MS-222. Clove oil did not have significant effects on levels of GLU, TP, CHOL, ALT and ALP. TGL concentration of fish exposed to 180mgL-1 clove oil was significantly higher than those of the rest anaesthetic groups. AST activities of fish exposed to 120, 150 and 180mgL-1 were significantly higher than those of 60 and 90mgL-1. Overall, TGL and AST could be potentially used as indicators of anaesthetic stress for juvenile Siberian sturgeon. Based on blood biochemical parameters, the appropriate anaesthetic concentrations of MS-222 and clove oil were 80-120mgL-1 and 60-90mgL-1, respectively. Clove oil was a promising alternative to MS-222. ?? 2011 Blackwell Verlag, Berlin.

  11. Vitamin D supplementation modulates blood and tissue zinc, liver glutathione and blood biochemical parameters in diabetic rats on a zinc-deficient diet.

    PubMed

    Kechrid, Zine; Hamdi, Malika; Naziro?lu, Mustafa; Flores-Arce, Manuel

    2012-09-01

    Previous studies suggest a protective effect of vitamin D3 on zinc deficiency-induced insulin secretion and on pancreas ?-cell function. The aim of this study was to investigate the effect of vitamin D on blood biochemical parameters, tissue zinc and liver glutathione in diabetic rats fed a zinc-deficient diet. For that purpose, Alloxan-induced diabetic rats were divided into four groups. The first group was fed a zinc-sufficient diet while the second group was fed a zinc-deficient diet. The third and fourth groups received zinc-sufficient or zinc-deficient diets plus oral vitamin D3 for 27 days. At the end of the experiment, blood, femur, pancreas, kidney and liver samples were taken from all rats. The serum, femur, pancreas, kidney and liver zinc concentrations, liver glutathione, serum alkaline phosphatase activity, daily body weight gain and food intake were lower in the zinc-deficient rats in comparison with those receiving adequate amounts of zinc. These values were increased in the zinc-deficient group that was supplemented with vitamin D3. The serum total cholesterol, triglycerides, total protein, urea, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and blood glucose values were higher in rats fed a zinc adequate diet, but their concentrations were decreased by vitamin D3 supplementation. The serum total protein levels were not changed by zinc deficiency and vitamin D3 supplementation. These results suggest that vitamin D3 modulates tissue zinc, liver glutathione and blood biochemical values in diabetic rats fed a zinc-deficient diet. PMID:22410949

  12. The dynamics of blood biochemical parameters in cosmonauts during long-term space flights

    Microsoft Academic Search

    Andrei Markin; Lubov Strogonova; Oleg Balashov; Valery Polyakov; Timoty Tigner

    1998-01-01

    Most of the previously obtained data on cosmonauts' metabolic state concerned certain stages of the postflight period. In this connection, all conclusions, as to metabolism peculiarities during the space flight, were to a large extent probabilistic.The purpose of this work was study of metabolism characteristics in cosmonauts directly during long-term space flights.In the capillary blood samples taken from a finger,

  13. Selected Haematological and Biochemical Parameters of Blood in Rats After Subchronic Administration of Vanadium and\\/or Magnesium in Drinking Water

    Microsoft Academic Search

    Agnieszka ?cibior; Halina Zaporowska; Jaros?aw Ostrowski

    2006-01-01

    The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological\\u000a and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as\\u000a a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg\\u000a V\\/mL; group

  14. Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance, blood biochemical parameters, and carcass characteristics.

    PubMed

    Zhou, Zhenming; Zhou, Bo; Ren, Liping; Meng, Qingxiang

    2014-01-01

    Fifty-one Simmental crossbred steers (357.0 ± 16.5 kg) were used to compare a standard total mix ration (TMR) with variants on animal performance, ruminal fermentation, blood biochemical parameters, and carcass characteristics. Corn grain and cotton seed meal were partially replaced by ensiled mulberry leaves (EML) or sun-dried mulberry fruit pomace (SMFP). Experimental diets had similar amounts of crude protein (CP), acid detergent fiber (ADF), and metabolizable energy (ME). Animals were divided into three groups: control group (CONT), 8% EML group, and 6.3% SMFP group. Performance, including average daily weight gain (ADG), and dry matter intake (DMI), was measured. Blood and rumen samples were collected at the end of the experiment (16 weeks). There were no differences in final body weight (P = 0.743), ADG (P = 0.425), DMI (P = 0.642), or ADG/DMI (P = 0.236) between the groups. There were no differences (P = 0.2024) in rumen pH values; ammonia N was lower (P = 0.0076) in SMFP than in the EML and CONT groups. There were differences in the concentrations of total and individual volatile fatty acids, while no differences were determined in blood biochemical parameters (i.e., plasma glucose, urea concentrations, triglycerides, total protein, insulin, IgG, alanine transaminase, and aspartate aminotransferase, P ? 0.098). No differences were observed in carcass characteristics (P ? 0.513), tenderness (P = 0.844), adipose and lean color values (P ? 0.149), and chemical composition (P ? 0.400); however, intramuscular fat was lower in the EML and SMFP groups compared to the CONT animals (P = 0.034). In conclusion, diets supplemented with these two mulberry products in an isocaloric and isonitrogenous manner have similar effects to corn grain and cotton seed meals on steer performance, blood biochemical parameters and carcass characteristics, with the exception of ruminal VFA concentrations and lower intramuscular fat content. PMID:24427304

  15. Changes in selected biochemical parameters in the kidney and blood of the fish, Tilapia mossambica (Peters), exposed to heptachlor

    Microsoft Academic Search

    V. Radhaiah; M. Girija; K. Jayantha Rao

    1987-01-01

    Pesticides used in pest control programs seem to produce many physiological and biochemical changes in fresh water organisms by influencing the activities of several enzymes. Alterations in the chemical composition of the natural aquatic environment usually effect behavioral and physiological systems of the inhabitants, particularly of the fish (0'Brlen 1967). Althongh some data are available on the effects of different

  16. Selected haematological and biochemical parameters of blood in rats after subchronic administration of vanadium and/or magnesium in drinking water.

    PubMed

    Scibior, Agnieszka; Zaporowska, Halina; Ostrowski, Jaros?aw

    2006-08-01

    The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg V/mL; group III, magnesium sulphate (MS) at a concentration of 0.06 mg Mg/mL; and group IV, SMV-MS solution at the same concentrations. The control group received at this time deionized water to drink. It was calculated that group II ingested with drinking water about 10.7 mg V/kg b. w./24 h, group III 6 mg Mg/kg b. w./24 h, and group IV about 9 mg V and 4.5 mg Mg/kg b. w./24 h. The exposure to vanadium alone (group II) led to a statistically significant decrease in body weight gain, food and fluid intakes. Moreover, in the same group of rats a statistically significant decrease in the RBC count, Hb concentration, MCV, and MCH values was demonstrated. Additionally, a statistically significant decrease in the plasma L-ascorbic acid concentration and a significant increase in MDA concentration in blood in this group were found. Instead, after the administration of magnesium alone (group III), a statistically significant decrease in the fluid intake and in the L-ascorbic acid concentration in plasma was noted. Furthermore, in the same group of rats a statistically significant increase in Hb level and in the plasma magnesium concentration was demonstrated. Two-way analysis of variance (ANOVA) did not reveal the interactions between V and Mg. PMID:16783625

  17. Bioimpedance spectroscopy as technique of hematological and biochemical analysis of blood

    NASA Astrophysics Data System (ADS)

    Malahov, M. V.; Smirnov, A. V.; Nikolaev, D. V.; Melnikov, A. A.; Vikulov, A. D.

    2010-04-01

    Bioimpedance spectroscopy may become a useful method for the express analysis and monitoring of blood parameters. The aim of this study was to identify biochemical and hematological parameters of blood that can be accurately predicted by means of bioimpedance technique. Hematological (red blood cell and white blood cell parameters) and biochemical (total proteins, albumins, fibrinogen, sodium, potassium, chloride ion concentrations in plasma) parameters were measured with a hematological analyzer and routine methods. Bioimpedance spectroscopy of the whole blood (1.5 ml) in frequency range 5-500 kHz (31 frequencies) was performed using BIA analyzer ABC-01 "Medass". Frequency relationships of resistance and reactance of the whole blood and the parameters of the Cole model were investigated. Close simple and multiple correlations of bioimpedance indices were observed only with erythrocyte parameters (Ht, Hb, RBC). Thus bioimpedance analysis of the whole blood can accurately predict red cell parameters but it is less effective for estimation of plasma biochemical and white cell parameters.

  18. Effect of Quinoa Seeds (Chenopodium quinoa) in Diet on some Biochemical Parameters and Essential Elements in Blood of High Fructose-Fed Rats

    PubMed Central

    Zagrodzki, Pawe?; Barto?, Henryk; Ch?opicka, Joanna; Gorinstein, Shela

    2010-01-01

    The effect of Chenopodium quinoa seeds on lipid profile, glucose level, protein metabolism and selected essential elements (Na, K, Ca, Mg) level was determined in high—fructose fed male Wistar rats. Fructose decreased significantly LDL [42%, p?blood of rats fed quinoa indicated, that these seeds effectively reduced serum total cholesterol [26%, p?

  19. Blood biochemical and cellular changes during decompression and simulated extravehicular activity

    NASA Technical Reports Server (NTRS)

    Jauchem, J. R.; Waligora, J. M.; Johnson, P. C. Jr

    1990-01-01

    Blood biochemical and cellular parameters were measured in human subjects before and after exposure to a decompression schedule involving 6 h of oxygen prebreathing. The exposure was designed to simulate extravehicular activity for 6 h (subjects performed exercise while exposed to 29.6 kPa). There were no significant differences between blood samples from subjects who were susceptible (n = 11) versus those who were resistant (n = 27) to formation of venous gas emboli. Although several statistically significant (P less than 0.05) changes in blood parameters were observed following the exposure (increases in white blood cell count, prothrombin time, and total bilirubin, and decreases in triglycerides, very-low-density lipoprotein cholesterol, and blood urea nitrogen), the changes were small in magnitude and blood factor levels remained within normal clinical ranges. Thus, the decompression schedule used in this study is not likely to result in blood changes that would pose a threat to astronauts during extravehicular activity.

  20. Blood Biochemical and Ruminal Liquor Profile in Buffaloes ( Bubalus bubalis ) Showing Omasal Impaction

    Microsoft Academic Search

    S. Turkar; S. K. Uppal

    2007-01-01

    The blood biochemical and ruminal fluid parameters of 5 buffaloes showing omasal impaction were studied, together with 10\\u000a healthy buffaloes as control. The diseased buffaloes had significantly higher serum aspartate aminotransferase, bilirubin,\\u000a glucose, blood urea nitrogen, creatinine, total protein, globulin and fibrinogen levels and significantly lower plasma calcium,\\u000a potassium and chloride concentrations than the controls. The ruminal liquor of the

  1. Haematological and Biochemical Values or the Blood and Blood Serum of Captive Northern White Rhinoceroses (Ceratotherium simum cottoni)

    Microsoft Academic Search

    J. Váhala; V. Kaše; O. A. Ryder

    1994-01-01

    Va h a I a 1., F. K d e and O. A. R Y d e r: Haematological and Biochemical Values of the Blood and Blood Serum of Captive Northern White Rhinoceroses (Ceratotherium simum cottoni). Acta vet. Brno, 63,1994: 99-102. Haematological and biochemical blood and blood serum values obtained from 7 northern white rhinoceroses (Ceratotherium simum cottoni) kept in

  2. The equation for prediction of blood viscosity from biochemical laboratory data

    NASA Astrophysics Data System (ADS)

    Sahin, B.; Yigitarslan, S.

    2015-03-01

    In most cases, the viscosity of blood is measured after adulteration with heparin or EDTA. The aim of the present research was to derive an equation that can be used for determination of viscosity from biochemical data. Blood samples taken from seven healthy people were analyzed in biochemical laboratory and their viscosities were measured by adding EDTA as anticoagulant. Ten parameters of biochemical laboratory including blood cells (erythrocytes, leukocytes, thrombocytes) and their functional products that directly affect the blood viscosity were chosen. Several equations relating viscosity to those parameters were derived by using a computer program. According to the regression analysis of the functions derived, the viscosity equation was obtained. This equation can be used for determination of blood viscosity from classical laboratory analysis. The advantages of using the derived equation are elimination of anticoagulant addition and elimination of Fahraeus_Lindquist effect. After proving that the equation is acceptable for numerous people, apparent viscosity changes can be followed during any disease and successfulness of anticoagulant drugs can be investigated.

  3. Biochemical and hormonal characteristics of peripheral blood in bulls in relation to genotype.

    PubMed

    G?owi?ska, Beata; Oler, Adam

    2013-01-01

    The study aimed at investigating differences in selected peripheral blood biochemical parameters and hormones in bulls of various genotype: Polish Holstein-Friesians (PHF; group I) and crossbreeds obtained from PHF dams sired by Limousin bulls (LMxPHF; group II). The blood for analysis was taken from the jugular vein twice. In blood serum the content of glucose, total protein, albumin, urea, total cholesterol, HDL, triglyceride, AST and ALT activity were determined. Insulin, triiodothyronine (T3) and thyroxine (T4) concentrations were also evaluated. The results showed that the insulin concentration and aspartate aminotransferase activity (AST) was significantly different (P<0.05) in both of the animal groups therefore it may be associated with bull genotype. Changes in triglyceride and urea levels were unclear thus long-term observation would be necessary to precisely interpret the relationships between blood serum parameters and bull genotype. PMID:23767296

  4. Blood biochemical and ruminal liquor profile in buffaloes (Bubalus bubalis) showing omasal impaction.

    PubMed

    Turkar, S; Uppal, S K

    2007-11-01

    The blood biochemical and ruminal fluid parameters of 5 buffaloes showing omasal impaction were studied, together with 10 healthy buffaloes as control. The diseased buffaloes had significantly higher serum aspartate aminotransferase, bilirubin, glucose, blood urea nitrogen, creatinine, total protein, globulin and fibrinogen levels and significantly lower plasma calcium, potassium and chloride concentrations than the controls. The ruminal liquor of the diseased buffaloes revealed characteristic physical, chemical and microbial changes and had significantly higher methylene blue reduction time and ammonia-nitrogen level. PMID:17294261

  5. Effect of Tamra Bhasma (Calcined Copper) on Ponderal and Biochemical Parameters

    PubMed Central

    Chaudhari, Swapnil Y.; Ruknuddin, Galib; Biswajyoti, J Patgiri; Kumar, Prajapati Pradeep

    2014-01-01

    Introduction: Tamra Bhasma (TB) and its forms like Somnathi Tamra Bhasma (STB), etc., are in vogue since centuries in Ayurveda. The present study is carried out to evaluate the effect of TB and STB in different dose levels on ponderal and biochemical parameters in wistar strain albino rats to provide scientific basis for its safety profile. Materials and Methods: TB and STB were prepared as per the classical guidelines and administered to wistar strain albino rats for 45 consecutive days. Blood was collected and rats were sacrificed on the 46th day. Ponderal and biochemical parameters were studied. Results: Results showed significant decrease in serum cholesterol, High Density Lipoprotein (HDL) cholesterol, triglycerides, total protein, and serum alkaline phosphatase levels. Comparatively, all the differences in between the groups are insignificant and no pathological changes at ponderal and biochemical levels were observed. Conclusion: Based on these observations, it can be said that these formulations can be safely used in cases of hyperlipidemia. PMID:25253924

  6. Longitudinal changes of biochemical parameters in muscle during critical illness

    Microsoft Academic Search

    Lena Gamrin; Kerstin Andersson; Eric Hultman; Eva Nilsson; Pia Essén; Jan Wernerman

    1997-01-01

    The study was undertaken to characterize the time course of biochemical parameters in skeletal muscle during critical illness to gain information for the design of a suitable protocol for interventional studies using metabolic or nutritional manipulation. Critically ill patients in our intensive care unit ([ICU] N = 9) were investigatedon two separate sampling occasions with percutaneous muscle biopsies for determination

  7. Parameter identifiability of power-law biochemical system models.

    PubMed

    Srinath, Sridharan; Gunawan, Rudiyanto

    2010-09-01

    Mathematical modeling has become an integral component in biotechnology, in which these models are frequently used to design and optimize bioprocesses. Canonical models, like power-laws within the Biochemical Systems Theory, offer numerous mathematical and numerical advantages, including built-in flexibility to simulate general nonlinear behavior. The construction of such models relies on the estimation of unknown case-specific model parameters by way of experimental data fitting, also known as inverse modeling. Despite the large number of publications on this topic, this task remains the bottleneck in canonical modeling of biochemical systems. The focus of this paper concerns with the question of identifiability of power-law models from dynamic data, that is, whether the parameter values can be uniquely and accurately identified from time-series data. Existing and newly developed parameter identifiability methods were applied to two power-law models of biochemical systems, and the results pointed to the lack of parametric identifiability as the root cause of the difficulty faced in the inverse modeling. Despite the focus on power-law models, the analyses and conclusions are extendable to other canonical models, and the issue of parameter identifiability is expected to be a common problem in biochemical system modeling. PMID:20197073

  8. Influence of dietary benzoic acid addition on nutrient digestibility and selected biochemical parameters in fattening rabbits

    Microsoft Academic Search

    G. Papadomichelakis; K. C. Mountzouris; E. Zoidis; K. Fegeros

    2011-01-01

    The effects of two inclusion levels of benzoic acid (5 and 20g\\/kg diet; B5 and B20, respectively) vs. a control (C) and an antibiotic (tiamulin) supplemented (A; 150mg\\/kg) diet on nutrient digestibility and selected biochemical parameters were investigated in 48 weaned (n=12 per treatment) rabbits (35 days old). Blood samples were obtained (at 45 and 85 days of age) to

  9. Identification of Streptococcus suis isolated from swine: proposal for biochemical parameters.

    PubMed Central

    Tarradas, C; Arenas, A; Maldonado, A; Luque, I; Miranda, A; Perea, A

    1994-01-01

    A study was made of the biochemical profiles of 59 strains serotyped as Streptococcus suis, isolated from diseased and clinically healthy pigs. The following parameters are proposed for the identification of the species: Voges-Proskauer negativity, hydrolysis of esculin positivity, trehalose positivity, negativity for growth in 6.5% NaCl, and absence of beta-hemolysis on sheep blood agar. S. suis serotype 2 is negative for hippurate, pyrrolidonylarylamidase, and mannose. PMID:8150982

  10. [Reference values of Russian cosmonauts blood biochemical indices].

    PubMed

    Markin, A A; Zhuravleva, O A; Morukov, B V; Kuzichkin, D S; Zabolotskaia, I V; Vostrikova, L V

    2013-01-01

    Within the period of 2000 to 2012 the values of 40 diagnostically significant biochemical indices in venous blood were being determined during the pre-flight clinical and physiological examination of 28 Russian cosmonauts aged 35 to 54, members of main and back-up crews of expeditions to the International Space Station. The above examination was conducted 45-30 days prior to the launch. Taking account of the fact that each of the most of the cosmonauts performed several flights over the mentioned period and they were repeatedly included in back-up crews over and over again, each of cosmonauts participated in pre-flight examinations 1 to 5 times. Reference values were calculated for each of the studied indices. It was found that reference interval boundaries for 15 indices were narrower as compared to the generally accepted ranges. The upper boundaries of activity reference ranges of a number of enzymes, as well as of concentration of some metabolites of energy and plasticity metabolism were raised relative to mean population values. Thus, it was found that Russian cosmonauts being a specific professional team featured inherent reference values which differed from mean population values in a number of blood biochemical indices. It is conditioned by peculiarities of selection, physical training and psychic and emotional status of crew members during a pre-flight period. PMID:23789387

  11. Biochemical parameters of plants as indicators of air pollution.

    PubMed

    Tripathi, A K; Gautam, Mukesh

    2007-01-01

    In the present study species like Mangifera indica, Linn., Cassia fistula, Linn., and Eucalyptus hybrid were exposed to different air pollution load for short duration (active biomonitoring). Variation in biochemical parameters like chlorophyll, protein, soluble sugar free amino acid, ascorbic acid, nitrate reductase, superoxide dismutase and peroxidase in the leaves were found to be pollution load dependent. These variations can be used as indicators of air pollution for early diagnosis of stress or as a marker for physiological damage to trees prior to the onset of visible injury symptoms. Just by analyzing these biochemical indicators air quality can also be assessed. PMID:17717999

  12. Blood-stimulatory and biochemical effects of intravenous laser irradiation of blood with low-active laser radiation in rabbits with peripheral nerve damage

    NASA Astrophysics Data System (ADS)

    Tanin, Leonid V.; Petrovsky, G. G.; Tanina, Raisa M.

    1996-12-01

    It is found that in rabbits with the injured sciatic nerve irradiation of blood with low-intensity laser radiation stimulates the mechanism of hemoglobin and thrombocyte formation. Normalization of the lymphocytic composition in blood of the animals with the injured nerve shows that it is possible to prevent development of the process of lymphocytopenia. Changes in the albumins/globulins ratio, the stimulating effect of same biochemical parameters and increase of the hemopoiesis in blood of the animals with the damaged peripheral nerve can probably be considered as evidence of the positive effect of intravenous irradiation of blood with low-intensity laser irradiation on living organisms.

  13. Biochemical parameters in Japanese quails Coturnix coturnix japonica infected with coccidia and treated with Toltrazuril.

    PubMed

    Sokól, R; Gesek, M; Ra?-Nory?ska, M; Michalczyk, M; Koziatek, S

    2015-01-01

    The activity of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase, total protein, albumin and cholesterol levels were determined in the blood serum of Japanese quails infected with coccidia and treated with Baycox (active ingredient: toltrazuril). Lower levels of AST and ALT activity were noted in treated birds regardless of the applied Baycox dose. The biochemical changes observed in the blood serum of Japanese quails point to coccidia-induced damage of digestive system tissues despite an absence of pronounced clinical symptoms. Significantly lower levels of AST activity and higher levels of LDH activity in treated birds indicate that coccidiosis treatment with toltrazuril contributed to the regeneration of digestive system tissues. An insignificant increase in cholesterol levels was noted, whereas the other serum biochemical parameters remained within the reference ranges. PMID:25928913

  14. Diazinon toxicity affects histophysiological and biochemical parameters in rabbits

    Microsoft Academic Search

    Mona A. H. Yehia; Sabah G. El-Banna; Aly B. Okab

    2007-01-01

    Diazinon is a widely used pesticide in agriculture. So, the current work aimed to investigate the effects of diazinon exposure on some physiological and biochemical parameters, as well as, histopathological changes and histochemical acetyl-cholinesterase activity (AChE). The red Baladi rabbits were dipped into water (Control Group), diazinon at low concentrations of 0.6mg diazinon low concentration (DLC) or high concentration of

  15. Effects of Housing Systems on Biochemical Indicators of Blood Plasma in Laying Hens

    Microsoft Academic Search

    A. Pavlík; M. Pokludová; D. Zapletal; P. Jelínek

    2007-01-01

    Pavlík A., M. Pokludová, D. Zapletal, P. Jelínek: Effects of Housing Systems on Biochemical Indicators of Blood Plasma in Laying Hens. Acta Vet. Brno 2007, 76: 339-347. The aim of this study was to compare biochemical indicators of blood plasma of laying hens housed in three different housing systems (conventional cage system, enriched cage system and deep litter system). In

  16. Age- and Sex-Based Hematological and Biochemical Parameters for Macaca fascicularis

    PubMed Central

    Zhou, Qinming; Wu, Qingyuan; Gong, Wei; Cheng, Ke; Li, Juan; Li, Leilei; Fang, Liang; Zhou, Linke; Xie, Peng

    2013-01-01

    Background The cynomolgus monkey (Macaca fascicularis) has been increasingly used in biomedical research, making knowledge of its blood-based parameters essential to support the selection of healthy subjects and its use in preclinical research. As age and sex affect these blood-based parameters, it is important to establish baseline indices for these parameters on an age and sex basis and determine the effects of age and sex on these indices. Methods A total of 917 cynomolgus monkeys (374 males and 543 females) were selected and segregated by age (five groups) and sex. A total of 30 hematological and 22 biochemical parameters were measured, and the effects of age and sex were analyzed. Results Baseline indices for hematological and biochemical parameters were separately established by age and sex. Significant effects by age, sex, and age-sex interaction were observed in a number of blood parameters. In the 49–60 months and 61–72 months age groups, red blood cell count, hemoglobulin, and hematocrit showed significantly lower values (P<0.01) in females than males. Serum alkaline phosphatase varied with age in both sexes (P<0.01) and was significantly higher in females than males (P<0.05) in the groups aged 13–24 months and 25–36 months; however, in the three groups aged over 25–36 months, serum alkaline phosphatase was significantly lower in females than males (P<0.01). Creatinine concentration increased with age (P<0.01) in all age groups; specifically in the groups aged 49–60 months and 61–72 months, creatinine was significantly higher (P<0.01) in males than females. Total protein and globulin both increased with age (P<0.01). Conclusion The baseline values of hematological and biochemical parameters reported herein establish reference indices of blood-based parameters in the cynomolgus monkey by age and sex, thereby aiding researchers in selecting healthy subjects and evaluating preclinical studies using this species. PMID:23762263

  17. Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea.

    PubMed

    Tomaszewska, Ewa; Winiarska-Mieczan, Anna; Dobrowolski, Piotr

    2015-01-01

    Rats were used to check whether regular consumption of black, red, or white tea would have a protective effect similar to the action of green tea on the intestine and liver in the case of exposure to Cd and Pb within the limits of human environmental exposure to these elements. Rats at the age of 6 weeks were divided into the control and four groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. Their diet (except the control) was mixed with 7mg Cd/kg and 50mg Pb/kg. The experiment lasted 12 weeks. The effects of administration of tea in Cd- and Pb-poisoned rats on plasma biochemical parameters and the jejunal epithelium and liver were determined. The highest body mass was found in the GT group. The highest hemoglobin and Fe concentrations were in the control and GT groups. The highest activity of AST was in groups poisoned with Cd and Pb independently on supplementation. The highest ALT activity was in BT and RT groups with lower content of polifenoles. Pb and Cd disturbed the liver leading to necrosis and fatty degenerative changes, and a loss of normal architecture of the hepatocytes. Rats from the GT group had the highest cell proliferation rate in intestinal glands and the largest absorptive surface. Black, red, and white tea exerted a varied impact on the histological structure and innervation of the small intestine wall as well as on the absorptive function of small intestine mucosa in rats poisoned with Pb and Cd than green tea. On the other hand, taking into account the number of apoptotic cells, the effect of the teas was the same. Moreover, it is clear that long term exposure to Cd and Pb contamination causes toxic effect in the liver. PMID:25837382

  18. Dynamics of blood plasma by spectropolarimetry and biochemical techniques

    NASA Astrophysics Data System (ADS)

    Voloshynska, Katerina; Ilashchuka, Tetjana; Prydij, Olexander; Gruia, Maria

    2014-08-01

    The aim of the study was to establish objective parameters of the field of laser and incoherent radiation of different spectral ranges (UV, visible, IR) as a non-invasive optical method of interaction with different samples of biological tissues and fluids of patients to determine the dynamics of metabolic syndrome and choosing the best personal treatment. As diagnostic methods have been used ultraviolet spectrometry samples of blood plasma in the liquid state, infrared spectroscopy middle range (2,5 - 25 microns) dry residue of plasma polarization and laser diagnostic technique of thin histological sections of biological tissues.

  19. Beneficial effects of mangiferin isolated from Salacia chinensis on biochemical and hematological parameters in rats with streptozotocin-induced diabetes.

    PubMed

    Sellamuthu, Periyar Selvam; Arulselvan, Palanisamy; Fakurazi, Sharida; Kandasamy, Murugesan

    2014-01-01

    Salacia chinensis L. is a traditional Southeast Asian herbal medicine and used in the treatment of diabetes. To investigate the antidiabetic properties of mangiferin from Salacia chinensis and its beneficial effect on toxicological and hematological parameters in streptozotocin induced diabetic rats. Mangiferin was orally treated with the dose of 40 mg/kg body weight/day for 30 days to diabetic rats. Biochemical (blood glucose, uric acid, urea and creatinine), toxicological (AST, ALT and ALP) and hematological parameters (red and white blood cells) and their functional indices were evaluated in diabetic treated groups with mangiferin and glibenclamide. Mangiferin treated diabetic rats significantly (p<0.05) lowered the level of blood glucose, in addition, altered the levels of biochemical parameters including urea, uric acid, and creatinine. Toxicological parameters including AST, ALT and ALP were also significantly reduced after treatment with mangiferin in diabetic rats. Similarly, the levels of red blood, white blood cells and their functional indices were significantly improved through the administration of mangiferin. Thus, our results indicate that mangiferin present in S. chinensis possesses antidiabetic properties and nontoxic nature against chemically induced diabetic rats. Further experimental investigations are warrant to make use of its relevant therapeutic effect to substantiate its ethno-medicinal usage. PMID:24374436

  20. Prognostic Significance of Various Biochemical Parameters in Acute Organophosphorus Poisoning

    PubMed Central

    Sumathi, M. E.; Kumar, S. Harish; Shashidhar, K. N.; Takkalaki, Nandini

    2014-01-01

    Background: Organophosphorus (OP) compounds are a heterogeneous group of insecticides widely used in agricultural industry. These OP compounds are likely to have more adverse effects in developing countries like India due to its easy availability and less awareness which results in high morbidity and mortality. Aims and objectives: 1. To estimate plasma cholinesterase, amylase, lipase and, creatine phosphokinase (CPK) in acute OP poisoning. 2. To correlate these biochemical parameters with plasma cholinesterase levels in OP poisoning. 3. To determine the use of a biochemical marker in predicting the severity of acute OP poisoning. Materials and Methods: A hospital based observational study was conducted on 53 subjects who have clinically diagnosed of acute OP poisoning and admitted in emergency unit of a tertiary care rural hospital. Subjects of either gender of all age-groups were included in the study. On admission, plasma cholinesterase, serum amylase, lipase and CPK were measured. Based on plasma cholinesterase activity at the time of admission, subjects were divided into three groups. Group I-having 20-50% of plasma cholinesterase activity; Group II-10-20% of plasma cholinesterase activity; and group III-<10% of plasma cholinesterase activity. Results: Serum amylase, lipase and CPK were negatively correlated with plasma cholinesterase levels. Serum amylase showed statistically significant negative correlation with plasma cholinesterase. Serum amylase showed the highest diagnostic accuracy for assessing severity of poisoning followed by CPK and Lipase. Conclusion: OP poisoning is associated with hyperamylasemia. Serum amylase, lipase and CPK can be used as an additional prognostic indicator with plasma cholinesterase levels. Serum amylase could be considered as a better predictor of severity followed by CPK and lipase. PMID:25253926

  1. Investigation of hematological and biochemical parameters in small ruminants naturally infected with Babesia ovis.

    PubMed

    Esmaeilnejad, Bijan; Tavassoli, Mousa; Asri-Rezaei, Siamak

    2012-01-01

    Babesia ovis plays an important role in causing anemia and kidney dysfunction in affected animals. There are few extensive studies about hematological and biochemical findings of small ruminants' babesiosis caused by B. ovis. The aim of this study was to evaluate the effect of babesiosis on some hematological and biochemical parameters in infected small ruminants with B. ovis. A total of 280 sheep and 122 goats from 40 herds were randomly examined for the presence of B. ovis in blood samples. Of 402 samples, 67 animals (16.7%) were positive for B. ovis of which 52 (18.5%) were sheep and 15 (12.2%) goats, respectively. The infected animals were divided into four subgroups according to parasitemia rates (<1%, 1%, 2%, and 3%). As a control group, 67 uninfected animals were also selected from the same farms. With increase in parasitemia rates, hemoglobin concentration (Hb), packed cell volume (PCV), red blood cells (RBCs), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) significantly decreased (P < 0.05), while, total leukocyte count, number of lymphocyte, monocyte, neutrophil and eosinophil showed a significant increase (P < 0.05). Infected animals presented a significant elevation (P < 0.05) of total proteins and significantly lower level (P < 0.05) of albumin compared to non-infected animals. Significant elevation (P < 0.05) of BUN, creatinine, cholestrol, triglyceride, HDL and LDL level were found with parasitemia progression. PMID:25653743

  2. Hematologic and serum biochemical parameters of apparently healthy rescued formosan pangolins (Manis pentadactyla pentadactyla).

    PubMed

    Chin, Shih-chien; Lien, Chen-yah; Chan, Yating; Chen, Chun-lin; Yang, Yi- ching; Yeh, Lih-seng

    2015-03-01

    Natural habitats of pangolins are rapidly deteriorating because of extensive farming, logging, and human construction activities. In addition, the illegal trading of pangolins substantially accelerated the decline of the pangolins' population in southeastern Asia. The maintenance of confiscated pangolins in rescue centers is currently a daunting task for veterinarians and conservation biologists. There is limited information in the literature about the reference values regarding the physiology of pangolins. The purpose of this study is to establish reliable hematologic and serum biochemical reference values for the Formosan pangolin (Manis pentadactyla pentadactyla). Blood samples were collected from 51 apparently healthy pangolins from a population of 117 rescued pangolins at the Taipei Zoo. Sex-related differences were observed in platelet count, alanine aminotransferase level, mean corpuscular hemoglobin concentration, and total protein level. Age-related differences were also noted; juveniles have significantly higher platelet counts and alkaline phosphatase levels than their adult counter parts. The hematologic and serum biochemical reference values for the Formosan pangolin presented in this study can be applied in the medical care of this important species during rescue attempts. It is the first systematic report of blood parameters of apparently healthy pangolins and provides a basis for future investigation of this species. The reference values reported in this study may also be applicable to other pangolin species in the genus Manis. PMID:25831578

  3. Investigation of hematological and biochemical parameters in small ruminants naturally infected with Babesia ovis

    PubMed Central

    Esmaeilnejad, Bijan; Tavassoli, Mousa; Asri-Rezaei, Siamak

    2012-01-01

    Babesia ovis plays an important role in causing anemia and kidney dysfunction in affected animals. There are few extensive studies about hematological and biochemical findings of small ruminants' babesiosis caused by B. ovis. The aim of this study was to evaluate the effect of babesiosis on some hematological and biochemical parameters in infected small ruminants with B. ovis. A total of 280 sheep and 122 goats from 40 herds were randomly examined for the presence of B. ovis in blood samples. Of 402 samples, 67 animals (16.7%) were positive for B. ovis of which 52 (18.5%) were sheep and 15 (12.2%) goats, respectively. The infected animals were divided into four subgroups according to parasitemia rates (<1%, 1%, 2%, and 3%). As a control group, 67 uninfected animals were also selected from the same farms. With increase in parasitemia rates, hemoglobin concentration (Hb), packed cell volume (PCV), red blood cells (RBCs), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) significantly decreased (P < 0.05), while, total leukocyte count, number of lymphocyte, monocyte, neutrophil and eosinophil showed a significant increase (P < 0.05). Infected animals presented a significant elevation (P < 0.05) of total proteins and significantly lower level (P < 0.05) of albumin compared to non-infected animals. Significant elevation (P < 0.05) of BUN, creatinine, cholestrol, triglyceride, HDL and LDL level were found with parasitemia progression. PMID:25653743

  4. Blood biochemical changes in pigs after infusion with acetate-buffered or lactate-buffered crystalloid solutions.

    PubMed

    Keibl, Claudia; Sipos, Wolfgang; Ponschab, Martin; Schlimp, Christoph J

    2015-06-19

    Perioperative fluid therapy is an important component of many medical procedures with animals. Buffered crystalloid solutions avoid inducing metabolic acidosis, but lactated solutions can elevate blood lactate concentrations and acetated solutions have not been thoroughly investigated using large animals. Here, the authors compare blood biochemical parameters in 20 juvenile pigs after perioperative fluid administration of an acetate-buffered solution (Elo-Mel isoton, EMI) or a lactate-buffered solution (lactated Ringer's solution, LRS). The authors measured blood lactate, glucose and electrolyte concentrations before and after administering the test fluid during surgery. Blood lactate concentration after administration was significantly higher in pigs that received LRS than in pigs that received EMI, but glucose and electrolyte concentrations did not differ significantly between treatment groups before or after administration. These findings suggest that EMI might be a preferable option for perioperative fluid therapy in pigs. PMID:26091132

  5. 2,4,5-Trichlororophenol and Its Derivatives Induce Biochemical and Morphological Changes in Human Peripheral Blood Lymphocytes In Vitro

    Microsoft Academic Search

    Jaromir Micha?owicz

    2010-01-01

    In this work, the investigation of the effects of 2,4,5-trichlorophenol (2,4,5-TCP), 4,6-dichloroguaiacol (4,6-DCG), and 4,5-dichlorocatechol\\u000a (4,5-DCC) on selected morphological and biochemical parameters in human peripheral blood lymphocytes were studied. All of\\u000a the investigated compounds (at concentrations from 25–600 ppm) increased the size and granularity of the lymphocytes. 2,4,5-TCP\\u000a induced the strongest and 4,5-DCC induced the weakest changes in these parameters. Moreover,

  6. VARIATION OF SOME BLOOD BIOCHEMICAL CHARACTERISTICS IN CATTLE, HORSES AND DOGS,

    E-print Network

    Paris-Sud XI, Université de

    VARIATION OF SOME BLOOD BIOCHEMICAL CHARACTERISTICS IN CATTLE, HORSES AND DOGS, AND CAUSES cattle. A few examples will also be given concerning horses and dogs. NORMAL RANGE In biology the concept the normal ranges for dairy cattle (Swedish Red and White Breed), horses (standardbred trotters) and dogs

  7. Propagation of Blood Clotting in the Complex Biochemical Network of Hemostasis Is Described by a Simple Mechanism

    E-print Network

    Ismagilov, Rustem F.

    Propagation of Blood Clotting in the Complex Biochemical Network of Hemostasis Is Described propagation of blood clotting from one vessel to another. The network of hemostasis controls blood clotting and localize blood clotting at sites of vascular injury. When this network fails to function correctly

  8. The effects of dietary chromium supplementation on some blood parameters in sheep

    Microsoft Academic Search

    Fatma Uyanik

    2001-01-01

    The effects of dietary inorganic chromium on some biochemical parameters were determined in lambs fed either a control diet\\u000a or a 200-ppb or 400-ppb chromium-supplemented diet. The live weight of the animals were measured and jugular blood samples\\u000a were collected prior to supplementation (d 0) and on d 20, 40, and 55. On d 55, three animals from each group

  9. Effects of Aqueous Bulb Extract of Allium sativum (Garlic) on Hematological and Biochemical Parameters in Rabbits: Experimental Trypanosoma brucei ssp. brucei Infection

    Microsoft Academic Search

    H. G. Mikail

    2009-01-01

    The influence of aqueous bulb extract of garlic, Allium sativum L., on hematological and biochemical parameters was investigated in rabbits infected with trypanosomosis. Blood samples collected 13 days after infection with trypanosomosis indicated that rabbits treated with the garlic extract had significantly higher packed cell volume (PCV) values compared with infected rabbits not treated with garlic. In addition, rabbits treated

  10. Gender-Specific Differences in Clinical Profile and Biochemical Parameters in Patients with Cushing's Disease: A Single Center Experience

    PubMed Central

    Zhu, Xiaoming; Zeng, Meifang; Zhuang, Yan; Zhou, Yiting; Zhang, Zhaoyun; Yang, Yehong; Wang, Yongfei; Ye, Hongying; Li, Yiming

    2015-01-01

    Cushing's disease (CD) is remarkably prevalent among females; however, more severe clinical presentation and adverse outcomes have been found in males. The purpose of this study was to investigate the overall clinical profile and biochemical parameters in patients with CD to identify the gender differences. Here we describe our series of CD patients referred to our medical center during 2012-2013. Among 73 cases, females presented a marked preponderance compared to males. Males had significantly higher ACTH, BMI, HbA1c, systolic blood pressure, and hemoglobin than females. For the first time, the incidence of fatty liver and hepatic function was also shown to be elevated in males. Multiple linear regression analysis was performed to further investigate the correlation of risk factors with hypokalemia, HbA1c, and systolic blood pressure. Gender and serum cortisol were associated with hypokalemia. Age, gender, and serum cortisol were significantly associated with HbA1c. Additionally, only gender was significantly associated with systolic blood pressure. Regarding clinical presentation, purple striae seemed to occur more frequently in males than in females. Thus, more severe clinical presentation, biochemical parameters, and complications were found in males than in females. Clinical professionals should pay more attention to the diagnosis and management of males with CD.

  11. Effects of therapeutic and toxic doses of levamisole on thyroid hormones and some biochemical parameters in sheep.

    PubMed

    Atessahin, Ahmet; Karahan, Izzet; Pirincci, Ibrahim

    2004-01-01

    This study was carried out to establish the effects of therapeutic and toxic doses of levamisole on thyroid hormone levels and some biochemical parameters in sheep. Twelve Akkaraman ewes were used. Levamisole was given orally at doses of 7.5 mg kg(-1) (group 1) and 40 mg kg(-1) (group 2) to the animals. Blood samples were taken from the jugular vein at 2, 4, 8, 24, 48, 96 and 144 h after the administrations. Serum thyroid hormones and some biochemical parameters were determined on these samples. When compared with the control levels, no significant changes were observed in triiodothyronine (T3) and thyroxin (T4) levels in group 1. Although levamisole was found to increase the levels of total T3, it decreased the levels of total T4 in group 2. On the other hand, free T3 and free T4 levels were not changed in either group. While serum alkaline phosphatase (ALP) activities were decreased, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and creatinine kinase (CK) activities were increased significantly by levamisole. However, it increased the serum albumin and cholesterol levels, but decreased the inorganic phosphate levels in groups 1 and 2. On the other hand, when compared with the control levels, no significant changes were detected in serum sodium, potassium and calcium levels. In conclusion, therapeutic and toxic doses of levamisole were determined to affect thyroid metabolism and some biochemical parameters in sheep. PMID:15338466

  12. Does Oral Coenzyme Q10 Plus NADH Supplementation Improve Fatigue and Biochemical Parameters in Chronic Fatigue Syndrome?

    PubMed Central

    Cordero, Mario D.; Segundo, María José; Sáez-Francàs, Naia; Calvo, Natalia; Román-Malo, Lourdes; Aliste, Luisa; Fernández de Sevilla, Tomás; Alegre, José

    2015-01-01

    Abstract Chronic fatigue syndrome (CFS) is a chronic and extremely debilitating illness characterized by prolonged fatigue and multiple symptoms with unknown cause, diagnostic test, or universally effective treatment. Inflammation, oxidative stress, mitochondrial dysfunction, and CoQ10 deficiency have been well documented in CFS. We conducted an 8-week, randomized, double-blind placebo-controlled trial to evaluate the benefits of oral CoQ10 (200?mg/day) plus NADH (20?mg/day) supplementation on fatigue and biochemical parameters in 73 Spanish CFS patients. This study was registered in ClinicalTrials.gov (NCT02063126). A significant improvement of fatigue showing a reduction in fatigue impact scale total score (p<0.05) was reported in treated group versus placebo. In addition, a recovery of the biochemical parameters was also reported. NAD+/NADH (p<0.001), CoQ10 (p<0.05), ATP (p<0.05), and citrate synthase (p<0.05) were significantly higher, and lipoperoxides (p<0.05) were significantly lower in blood mononuclear cells of the treated group. These observations lead to the hypothesis that the oral CoQ10 plus NADH supplementation could confer potential therapeutic benefits on fatigue and biochemical parameters in CFS. Larger sample trials are warranted to confirm these findings. Antioxid. Redox Signal. 22, 679–685. PMID:25386668

  13. Effect of fire smoke on some biochemical parameters in firefighters of Saudi Arabia

    Microsoft Academic Search

    Abdulrahman L Al-Malki; Ameen M Rezq; Mohamed H Al-Saedy

    2008-01-01

    BACKGROUND: Firefighters who are facing fires, are frequently exposed to hazardous materials including carbon monoxide, hydrogen cyanide, hydrogen chloride, benzene, sulphur dioxide, etc. This study aimed to evaluate some relevant serum biochemical and blood hematological changes in activity involved firefighters in comparison to normal subjects. SUBJECTS AND METHODS: Two groups of male firefighters volunteered to participate in the study. The

  14. Biochemical evaluation of naphthalene intoxication in the tropical acrid blood clam Anadara granosa

    Microsoft Academic Search

    B. Patel; J. T. Eapen

    1989-01-01

    Exposure of the tropical arcid blood clam Anadara granosa L., collected from Bombay waters in 1986, to sublethal concentrations of naphthalene (Nap) (5, 10 and 15µg Nap ml-1), induced various changes in vital biochemical systems. The condition index (CI) remained largely within the control range, although tissue water content after 96h exposure increased significantly (pppp«0.001) by more than 60% with

  15. Toxicity Assessment of Two Soils from Jales Mine (Portugal) Using Plants: Growth and Biochemical Parameters

    Microsoft Academic Search

    Susana Loureiro; Glória Pinto; Armando Costa; Marta Monteiro; António J. A. Nogueira; Amadeu M. V. M. Soares

    2006-01-01

    Contaminants in soils can enter food chains through primary producers. Bioavailable contaminants can induce growth, and reproductive\\u000a or biochemical changes in plants. To evaluate the bioavailability of heavy metals in two soils from Jales mine surroundings,\\u000a bioassays with the plants Brassica rapa (RCBr) and Avena sativa were performed. Biochemical parameters (protein and malondialdehyde [MDA] content, and catalase and peroxidase activities)

  16. Novel parameters in blood cell counters.

    PubMed

    Lecompte, Thomas Pierre; Bernimoulin, Michael Pierre

    2015-03-01

    Many modern automated cell counters offer novel parameters. Some of these, such as the CHr, reticulocyte hemoglobin equivalent, or immature platelet fraction, schistocytes have been accepted by many clinicians; the CHr has even become part of clinical practice guidelines for dialysis patients. Other parameters are still research-use only and lack well-defined reference ranges. One important albeit rare clinical problem is to characterize platelet volume in case of inherited thrombocytopenia. Most novel parameters are specific for certain instrument platforms, and results of even similar parameters from different manufacturers are often difficult to compare. PMID:25676381

  17. Effects of levothyroxine treatment on biochemical and hemostasis parameters in patients with hypothyroidism

    Microsoft Academic Search

    Sevim Gullu; Hasan Sav; Nuri Kamel

    2005-01-01

    Objective: The aims of the study were to evaluate the disturbances in the coagulation system in patients with overt hypothyroidism (OH), to assess the effects of levothyroxine (LT4) on the coagu- lation parameters, and to determine whether subclinical hypothyroidism (SH) affects concentrations of coagulation markers and several biochemical parameters, thereby supporting early substitution. Design: The study included 15 patients with

  18. Alterations in biochemical parameters during subacute toxicity of fluoride alone and in conjunction with aluminum sulfate in goats.

    PubMed

    Kant, Vinay; Srivastava, Anil Kumar; Verma, Pawan Kumar; Raina, Rajinder

    2009-07-01

    Fluoride toxicity is a serious health problem in many parts of the globe. In present study, sodium fluoride at 20 mg/kg alone and in conjunction with aluminum sulfate at 150 mg/kg was administered orally daily for 30 days in healthy goats of group 1 and 2, respectively, to access the alterations in the various biochemical parameters during subacute toxicity of fluoride alone and in conjunction with aluminum sulfate. In Group 1, significant alterations in plasma glucose, blood urea nitrogen (BUN), creatinine, total protein, albumin, globulin, albumin/globulin ratio, magnesium, and sodium were observed on different days of exposure from their pre-exposure values. However, no significant changes were observed in plasma calcium, phosphorus, and potassium on different days of exposure of sodium fluoride. Similar type of biochemical alterations were noticed in the goats of Group 2 except BUN, total protein magnesium, and sodium. On the basis of results, it could be concluded that sodium fluoride alone and in conjunction with aluminum sulfate produced significant alterations in the various biochemical parameters of the body. PMID:19148585

  19. Effect of storage on biochemical and microbiological parameters of edible truffle species.

    PubMed

    Saltarelli, Roberta; Ceccaroli, Paola; Cesari, Paola; Barbieri, Elena; Stocchi, Vilberto

    2008-07-01

    The effects of different storage treatments on the most common edible truffle species, such as Tuber magnatum and Tuber borchii (white truffles), Tuber melanosporum and Tuber aestivum (black truffles), were analysed. Biochemical and microbiological profiles were monitored, in order to evaluate possible alterations during truffle preservation. After harvesting, some fresh samples were kept at 4°C for 30days, other samples were frozen at -20°C for one month, thawed and preserved at 4°C; the remainder were autoclaved. The biochemical parameters studied were sugar and protein content, the activity of some enzymes involved in the central metabolism of the fungi and the electrophoretic pattern of soluble proteins. Total mesophilic bacteria were also counted. The results obtained showed that the storage at 4°C is the treatment that best preserves the biochemical and microbiological characteristics of fresh truffles. Black truffles were more resistant to biochemical spoilage than the white ones, while T. magnatum was the most resistant to microbial spoilage. PMID:26054259

  20. Blood biochemical and cellular changes during a decompression procedure involving eight hours of oxygen prebreathing

    NASA Technical Reports Server (NTRS)

    Jauchem, J. R.

    1989-01-01

    Chemical and cellular parameters were measured in human subjects before and after exposure to a decompression schedule involving 8 h of oxygen prebreathing. The exposure was designed to simulate space-flight extravehicular activity (EVA) for 6 h. Several statistically significant changes in blood parameters were observed following the exposure: increases in calcium, magnesium, osmolality, low-density lipoprotein cholesterol, monocytes, and prothrombin time, and decreases in chloride, creatine phosphokinase and eosinophils. The changes, however, were small in magnitude and blood factor levels remained within normal clinical ranges. Thus, the decompression profile used in this study is not likely to result in blood changes that would pose a threat to astronauts during EVA.

  1. Assessment of gallstone predictor: comparative analysis of ultrasonographic and biochemical parameters

    PubMed Central

    2013-01-01

    Background Gallstones represent a significant burden for health care systems worldwide and are one of the most common disorders presenting to emergency room. Ultrasonography, complete blood picture test and liver function tests are procedures of choice in suspected gallstones or biliary diseases. They are the most sensitive, specific, non-invasive and inexpensive tests for the detection of gallstones. Our main objective was to evaluate the relationship of ultrasonographic findings, hemolytic indices and liver function tests with gallstones. Methodology It was a prospective study carried out in Civil Hospital Karachi (DUHS) and Liaquat National Hospital, two largest tertiary care hospitals of Karachi, Pakistan. Duration of the study was from July 2011 to October 2012. The study was carried out on diagnosed, pre-operative and symptomatic patients of cholelithiases. Exclusion criteria were patients of gallbladder and pancreatic carcinoma, emergency operations, patients having age <12 years and non-cooperative patients, who refused to give written consent for participation in the study. Total two tests were performed on each patient after diagnosis by ultrasonography. These were complete blood count and liver function tests. All the demographic data, laboratory findings and ultrasonographic features were noted in a pre-structured Performa. Sample size was calculated by using open-epidemiological sample size calculator prevalence (p)?=?35%, d?=?5%, and confidence interval (CI) 95%?=?350. All the data was entered and analyzed through SPSS 19. Result There were 454 diagnosed and pre-operative cases of gallstones present in the study. There were 120(26.4%) males and 334(73.6%) females, with a mean age of 42.80?±?12.26 years. Most of the suspects had multiple stones 384 (84.5%) while few had single stones 70(15.4%). Fatty liver was found to be present in 144(31.7%) patients and 92(20.2%) had hepatomegaly. Splenomegaly was present in 16(3.5%) patients. Alkaline phosphatase was elevated in 186(41.0%) patients while SGPT was found to be raised in 160(35.2%). Blood urea nitrogen was found to be elevated in 186(41%) patients and serum creatinine was elevated in 46(10.1%) patients. Conclusion In the light of findings it is recommend that all patients should go through the process of ultrasonography and all the biochemical parameters should be analyzed before surgery. PMID:23618353

  2. Serum Biochemical Parameters in the Ring-Necked Pheasant (Phasianus colchicus) on Breeding Season

    Microsoft Academic Search

    Elizabeth Moreira dos Santos Schmidt; Antonio Carlos Paulillo; Rosangela Locatelli Dittrich; Elizabeth Santin; Olair Beltrame; Joelma Moura; Edson Goncalves de Oliveira

    2007-01-01

    5 Abstract: The influence of sex on serum biochemical parameters was investigated in adult (52 week-old) ring-necked pheasants on reproductive activity. Statistical comparisons were made for differences in values between male and female birds. Adult females had higher values for serum total protein and albumin. Significant variations were not observed in the analyses in relation to the sex of the

  3. Effect of Nigella Sativa oil on various clinical and biochemical parameters of metabolic syndrome

    Microsoft Academic Search

    A Najmi; SF Haque; M Naseeruddin; RA Khan

    Background: The seeds of Nigella sativa plant have been used to promote health and fight disease for centuries especially in the Middle East and Southeast Asia. This plant has been a great focus of research. This clinical study was undertaken to determine the adjuvant effect of Nigella sativa oil on various clinical and biochemical parameters of the metabolic syndrome. Methodology:

  4. Biological, biochemical, and molecular parameters of Helisoma duryi snails exposed to the pesticides Malathion and Deltamethrin

    Microsoft Academic Search

    Fayez A. Bakry; Wafaa S. Hasheesh; Salwa A. H. Hamdi

    2011-01-01

    Aquatic environments get contaminated with pesticides residues that result from the application of pesticides in agricultural practices. The present study aimed to evaluate the pesticides Malathion and Deltamethrin on biological and biochemical parameters of Helisoma duryi snails. The results showed that LC10 of the two pesticides caused considerable reduction in survival rates and egg production of treated snails. Glucose concentration

  5. Effect of Pregnancy on Some Biochemical Parameters in Sahel Goats in Semi-arid Zones

    Microsoft Academic Search

    U. K. Sandabe; A. R. Mustapha; E. Y. Sambo

    2004-01-01

    The effects of pregnancy one some biochemical parameters were studied using 20 sexually mature, cycling goats with weight range 20–25 kg. They were randomly separated into two groups of 10 animals each. In one group, oestrus was detected while going round with a buck in the morning and evening; a single buck on detection of oestrus mated the does and

  6. Biochemical and Hemato-immunological Parameters in Juvenile Beluga (Huso huso) Following the Diet Supplemented with Nettle (Urtica dioica).

    PubMed

    Binaii, Mohammad; Ghiasi, Maryam; Mohammad, Seyed; Farabi, Vahid; Pourgholam, Reza; Fazli, Hasan; Safari, Reza; Alavi, Seyed Eshagh; Taghavi, Mohammad Javad; Bankehsaz, Zahra

    2013-10-24

    The present study investigated the effects of different dietary nettle (Urtica dioica) levels on biochemical, hematological and immunological parameters in beluga (Huso huso). Fish were divided into 4 groups before being fed for 8 weeks with 0%, 3%, 6% and 12% of nettle. The blood samples were collected on week 4 and 8. The use of nettle were not significantly changed the mean cell volume , mean cell haemoglobin, lymphocytes, eosinophils, albumin, glucose, alanine aminotransferase , aspartate aminotransferase, alkaline phosphatase and lysozyme activity on week 4 and 8. After 4 weeks, the total red blood cell (RBC) and haematocrit (Ht) showed a significant increase in 12% nettle group compared to the 3% nettle and control groups but haemoglobin (Hb) had a significant change in 12% nettle compared to the control. At the same time was not found a significant change in the mean cell haemoglobin concentration (MCHC), total white blood cell (WBC), neutrophils, respiratory burst activity (RB), total immunoglobulin (Ig) and total protein (TP), triglyceride (Tri) and cholesterol (Chol). After 8 weeks, the fish treated with nettle exhibited significantly increase in neutrophil and Hb levels compared to the control and between treatment groups, 12% nettle group shown the highest Hb while RBC and Hct values significantly rose in fish fed by 12% compared to the control. Supplementing %6 and 12% nettle increased the WBC and MCHC compared the other groups. The group fed 12% showed a highly significant difference in RB, TP and Ig after 8 weeks. However, Tri and Chol were significantly decreased in the juvenile beluga fed by the %6 and 12% nettle diet compared the other groups. The results suggest that by using this herb there will be an improvement hemato- biochemical parameters and immune function of juvenile beluga. PMID:24513497

  7. Biochemical and hemato-immunological parameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica).

    PubMed

    Binaii, Mohammad; Ghiasi, Maryam; Farabi, Seyed Mohammad Vahid; Pourgholam, Reza; Fazli, Hasan; Safari, Reza; Alavi, Seyed Eshagh; Taghavi, Mohammad Javad; Bankehsaz, Zahra

    2014-01-01

    The present study investigated the effects of different dietary nettle (Urtica dioica) levels on biochemical, hematological and immunological parameters in beluga (Huso huso). Fish were divided into 4 groups before being fed for 8 weeks with 0%, 3%, 6% and 12% of nettle. The blood samples were collected on week 4 and 8. The use of nettle did not significantly change the mean cell volume, mean cell haemoglobin, lymphocytes, eosinophils, albumin, glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lysozyme activity on week 4 and 8. After 4 weeks, the total red blood cell (RBC) and hematocrit (Ht) showed a significant increase in 12% nettle group compared to the 3% nettle and control groups but haemoglobin (Hb) had a significant change in 12% nettle compared to the control. At the same time was not found a significant change in the mean cell haemoglobin concentration (MCHC), total white blood cell (WBC), neutrophils, respiratory burst activity (RB), total immunoglobulin (Ig) and total protein (TP), triglyceride (Tri) and cholesterol (Chol). After 8 weeks, the fish treated with nettle exhibited significantly increase in neutrophil and Hb levels compared to the control and between treatment groups, 12% nettle group shown the highest Hb while RBC and Hct values significantly rose in fish fed by 12% compared to the control. Supplementing 6% and 12% nettle increased the WBC and MCHC compared to the other groups. The group fed 12% showed a highly significant difference in RB, TP and Ig after 8 weeks. However, Tri and Chol were significantly decreased in the juvenile beluga fed by the 6% and 12% nettle diet compared to the other groups. The results suggest that by using this herb there will be an improvement in hemato-biochemical parameters and immune function of juvenile beluga. PMID:24516872

  8. Saline or plant-incorporated methylmercury effects on distribution, demethylation, and blood parameters in rats

    SciTech Connect

    Czuba, M.; Komsta-Szumska, E.; Mortimer, D.C.; Champagne, C.

    1987-03-01

    The influence of diet is recognized as a significant factor in the expression of toxicity. This applies particularly to toxins like methylmercury (MeHg) which are metabolically incorporated into growing food plants and biotransformed within the plant before ingestion. Methylmercury in this form may influence the early physiological and biochemical events which lead to development of toxicity. In a previous study, a single dose of plant-incorporated methylmercury (MeHg) had a different route of distribution and accumulation in rat organs after 48 h than an equivalent dose of saline MeHg with the greatest accumulation being in red blood cells. Creatine, an important storage form of high energy phosphate in muscles is a primary indicator of erythropoietic dynamics under hypoxia, a sensitive indicator of hemolytic disease, red blood cell aging and impaired marrow efficiency. Since changes in creatine levels occur sooner than changes in other blood parameters, itself being neither synthesized nor metabolized by red blood cells, it can be used as a sensitive indicator of toxicity. The present work investigates the difference in a longer term, multiple-dose regime of saline or bean-incorporated MeHg ingestion with special attention being given to various blood parameters.

  9. [The blood biochemical indicators as possible markers of destruction of kerato-hyaline tissue of nails in patients with onycholysis].

    PubMed

    Urazovskaia, E V; Mikashinovich, Z I

    2012-12-01

    It is established that the most informative blood biochemical indicators of destructive process in patients with onycholysis are to be considered concentration of oxiproline in blood. The changing of ratio of fractions oxiproline, balance between concentration of enzymes of antioxidant defense and metalloproteinase and concentration of hemoglobin in blood make it possible to diagnose the mixed type of hypoxemia which is a possible cause of destruction of kerato-hyaline tissue in patients with onycholysis. PMID:23479963

  10. The influence of administering "effective microorganisms" to pullets on chosen haematological and biochemical blood indexes.

    PubMed

    Sokó?, R; Michalczyk, M; Spodniewska, A; Barski, D

    2009-01-01

    "Effective Microorganisms" (EM)--a mixture of lactic acid bacteria, photosynthetic bacteria, yeasts and fungi are used mainly in agriculture and organic waste treatment. Recently, they have also been added to water and feed for animals, as well as to processing their excrements into compost and to eliminate the stench. The objective of the present study was to assess the influence of a 14-day administration of an EM solution in drinking water to layer hens on chosen haematological and biochemical indexes. The research was carried out on 120 hens divided into two equal groups. The birds in the experimental group were given drinking water with dissolved EM (5% solution), and those in the control group--water without the preparation. On the 64th day of the aviculture, the hens were weighted and their blood was taken from the wing vein for haematological and biochemical examinations. Administering EM with water to hens did not influence significantly their body weight nor chosen haematological and biochemical indexes. A significant increase was found only in the number of platelets, the level of albumins, the content of total cholesterol and the LDH activity, however, a decrease in the ALT activity was observed. PMID:20169927

  11. A Model Incorporating Some of the Mechanical and Biochemical Factors Underlying Clot Formation and Dissolution in Flowing Blood

    DOE PAGESBeta

    Anand, M.; Rajagopal, K.; Rajagopal, K. R.

    2003-01-01

    Multiple interacting mechanisms control the formation and dissolution of clots to maintain blood in a state of delicate balance. In addition to a myriad of biochemical reactions, rheological factors also play a crucial role in modulating the response of blood to external stimuli. To date, a comprehensive model for clot formation and dissolution, that takes into account the biochemical, medical and rheological factors, has not been put into place, the existing models emphasizing either one or the other of the factors. In this paper, after discussing the various biochemical, physiologic and rheological factors at some length, we develop a modelmore »for clot formation and dissolution that incorporates many of the relevant crucial factors that have a bearing on the problem. The model, though just a first step towards understanding a complex phenomenon, goes further than previous models in integrating the biochemical, physiologic and rheological factors that come into play.« less

  12. Influence of Calicophoron microbothrium amphistomosis on the biochemical and blood cell counts of cattle.

    PubMed

    Mavenyengwa, M; Mukaratirwa, S; Monrad, J

    2010-12-01

    Sixteen Tuli steers aged 1 year were subdivided into four equal groups (I-IV) and infected with Calicophoron microbothrium metacercariae. Group I received a low dose (LD) of 5000 metacercariae, group II a medium dose (MD) of 15,000 metacercariae, group III a high dose (HD) of 25,000 metacercariae while group IV was the non-infected control (C) group. The experimental animals were monitored daily for clinical signs while ethylene diamine tetraacetic acid (EDTA) blood and serum samples were collected every 7 days until day 28 post-infection, when sample collection was terminated. Samples were processed for full blood count, eosinophils and blood biochemical values for calcium, magnesium, phosphorus, total protein and albumin. Moderate to severe diarrhoea developed in the MD and HD groups at day 21 post-infection. The diarrhoea coincided with a significant decrease (P < 0.05) in total plasma protein, calcium and phosphorus levels, particularly in the MD group. Similarly, a significant decrease (P < 0.05) in the packed cell volume (PCV), the haemoglobin (Hb) and red blood cell (RBC) levels occurred in the MD and HD groups from day 21 post-infection, while a significant increase (P < 0.05) in the circulating eosinophils occurred between 7 and 21 days post-infection in the LD and the HD groups. PMID:20109245

  13. Stability of Intercellular Exchange of Biochemical Substances Affected by Variability of Environmental Parameters

    NASA Astrophysics Data System (ADS)

    Mihailovi?, Dragutin T.; Budin?evi?, Mirko; Balaž, Igor; Mihailovi?, Anja

    Communication between cells is realized by exchange of biochemical substances. Due to internal organization of living systems and variability of external parameters, the exchange is heavily influenced by perturbations of various parameters at almost all stages of the process. Since communication is one of essential processes for functioning of living systems it is of interest to investigate conditions for its stability. Using previously developed simplified model of bacterial communication in a form of coupled difference logistic equations we investigate stability of exchange of signaling molecules under variability of internal and external parameters.

  14. The influences of SE infection on layers’ production performance, egg quality and blood biochemical indicators

    PubMed Central

    2014-01-01

    Background Salmonella enterica serovar Enteritidis (SE), as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers’ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into 2 groups, 60 hens for experiment and others for control. The experiment group was fed with the dosage of 108 CFU SE per hen. The specific PCR was used to detect the deposition of SE. On the 8 d after SE infection, 10 hens from the control group and 30 hens from the experimental group were slaughtered to detect the SE colonization. The production performance, egg quality and blood biochemical indices were also analyzed. Results The results showed that the colonization rate of SE was highest in caecum contents (55.17%) and lowest in vagina (17.24%). For the eggs the detection rate of SE was highest on the eggshell (80.00%) and lowest in yolk (18.81%). SE infection had no significant influence on production performance and egg qualities (P > 0.05). The difference of laying rate between the experimental and control groups was less than 0.30%, and both were approximately equal to 82.00%. The blood analysis showed that the aspartic aminotransferase (AST) and alanine aminotransferase (ALT) of experimental group was significantly higher than those of control group (P < 0.05). For experimental and control groups AST values were 236.22 U/l and 211.84 U/l respectively, and ALT values were 32.19 U/l and 24.55 U/l. All of coefficients were less than 20%. The colonization of SE in organs increases the enzyme activities of AST and ALT in blood. Conclusions SE in feed could invade the oviduct and infect the forming eggs. It significantly increased the concentration of ALT and AST in blood. However,SE infection was hard to be observed from the appearances of layer and egg. It might be a dangerous risk to human health. PMID:24405886

  15. Effect of Chronic High Dose-Alcohol Consumption on the General Biochemical Parameters

    Microsoft Academic Search

    Ramazan Amanvermez; Seyit Ankarali; Özgür K. Tunçel; Leman Tomak; Muhlise Alvur

    2009-01-01

    Objectives: A chronic intake of high dose alcohol may cause oxidative stress, meta- bolic abnormalities and nutritional deficiencies in the body. The effect of long-term alcohol consumption on the biochemical parameters has not been explained well. Materials and Methods: Female and male rats were maintained for 90 days as follow: I. Control (n=7), II. Alcohol-treated (2.5 gr of 50% ethanol\\/kg

  16. Impact of industrial air pollutants on some biochemical parameters and yield in wheat and mustard plants

    Microsoft Academic Search

    Namita Joshi; Avnish Chauhan; P. C. Joshi

    2009-01-01

    The present study was carried out to determine the impacts of SO2, NO\\u000a x\\u000a , SPM and RSPM, the most common air pollutants, generated mainly due to industries and vehicles, on some biochemical parameters\\u000a and yield in wheat and mustard plants during 2006. The concentration of SO2, NO\\u000a x\\u000a , SPM and RSPM was determined at the polluted sites across

  17. Impact of environmental stress on biochemical parameters of bacteria reducing chromium

    PubMed Central

    Batool, Rida; Yrjälä, Kim; Hasnain, Shahida

    2014-01-01

    Chromium pollution is produced in connection with industrial processes like in tanneries. It has been suggested that bioremediation could be a good option for clean up. The stress effect of variable chromate levels, pHs and growth temperatures on biochemical parameters of two Cr(VI) reducing bacterial strains Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium Rb-2 was investigated. Transmission electrone microscopy (TEM) was performed to study the intracellular distribution of Cr(VI). It was observed that initial stress of 1000 ?gmL?1 caused significant enhancement of all studied biochemical parameters at pH 7.0 and growth temperature of 37 °C showing great bioremediation potential of the strains. Transmission electron microscopy revealed that the distribution of chromium precipitates was not uniform as they were distributed in the cytoplasm as well as found associated with the periplasm and outer membrane. Fourier transform infrared spectroscopy showed the possible involvement of carboxyl, amino, sulpohonate and hydroxyl groups present on the bacterial cell surface for the binding of Cr(VI) ions. Cr(VI) stress brought about changes in the distridution of these functional groups. It can be concluded that the investigated bacterial strains adjust well to Cr(VI) stress in terms of biochemical parameters and along that exhibited alteration in morphology. PMID:25242944

  18. Comparative aspects of biochemical polymorphism in the blood of Caprinae species and their hybrids.

    PubMed

    Tucker, E M; Clarke, S W

    1980-01-01

    Biochemical variation at 14 blood loci was reviewed, and specific features compared experimentally in sheep Ovis aries, mouflon Ovis musimon, goat Capra hircus, aoudad Ammotragus Iervia and in 2 stillborn aoudad X goat hybrids. Variation at 3 loci was also studied in dall sheep Ovis dalli, bighorn sheep Ovis canadensis and rocky mountain goat Oreamnos americanus. Haemoglobin C production in an anaemic Hb AB mouflon and in mouflon X sheep hybrids was examined. Mouflon differ from domestic sheep in that synthesis of both Hb beta A and Hb beta B chains is switched off during Hb C production. The mouflon X sheep hybrids switched off one or both chains depending on whether they had inherited sheep or mouflon Hb beta chain genes. In general aoudad showed a closer affinity to goats than to sheep. PMID:7458003

  19. Evaluation of the toxicity of cypermethrin pesticide on organs weight loss and some biochemical and histological parameters.

    PubMed

    Dahamna, S; Belguet, A; Bouamra, D; Guendouz, A; Mergham, M; Harzallah, D

    2011-01-01

    An increase in global food demand has resulted in a significant increase in the use of pesticides in agriculture. Synthetic pyrethroid pesticides account for over 30% of the global pesticide use; Pyrethroid pesticides were used preferably over organochlorines and organophosphates due to their high effectiveness, low toxicity to non-target organisms and easy biodegrability. It has widespread applications in agriculture through the world and as well in Algeria. Cypermethrin is one of the most insecticidal pyrethroids widely used in agriculture regions of Setif. to control wide range of insect pests in a variety of crops. The aim of this study is to investigate the effects of cypermethrin (Cyper-Ac 271 g/l from the active substance of the cypermethrin) on hematological, biochemical parameters, body weight loss, and histopathological study of some organs. Male mice weighing 30-40g were used, separated in 5 groups, n=6, two groups controls given vehicle (oil vegetable) and three experimental groups (Cypermetherin and vegetable oil). The animals were gavaged by 1/5 LD50 (LD50 = 485 mg/kg b/w) for 2 and 4 weeks respectively, and with 1/20 LD50 for 12 weeks, then the animals sacrificed at the end of the experiment.. Blood was collected. Enzyme activities were assayed in the plasma samples obtained. Glutamate oxaloacetate transaminase (GOT), Glutamate pyruvate transaminase (GPT), Alkaline phosphatase (ALPH) and Glucose. Red blood cells, (RBC), and white blood cells (WBC) were calculated too. The samples of liver and kidney were processed for histology. The results indicated a significant increase in transaminases GOT, GPT, and AlP. The decrease in Hb, RBC and WBC which are related to the immunity, this is probably due to cell lyses explain the effect of Cypermetherin on erythropoeisis. cypermethrin treatment exhibited severe histopathological changes, especially in the liver and kideney accompanied by weight loss of some organs. We conclude that cypermethrin induces oxidative stress and modifies biochemical parameters and histological aspects of liver and kidney. PMID:22702208

  20. Rosa canina L. Fruit Hydro-Alcoholic Extract Effects on Some Immunological and Biochemical Parameters in Rats

    PubMed Central

    Sadigh-Eteghad, Saeed; Tayefi-Nasrabadi, Hossein; Aghdam, Zahra; Zarredar, Habib; Shanehbandi, Dariush; Khayyat, Leila; Seyyed-Piran, Seyyed-Hamed

    2011-01-01

    Introduction This research investigates the possible potential of Rosa canina (RC) as an immunomodulator in rats and its effects on some biochemical parameters. Methods In this experiment, 45 male Wistar rats were obtained and divided into three groups (n = 15). These groups received normal saline (10 mg/kg), RC fruit extract (250 mg/kg) and RC fruit extract (500 mg/kg) as oral gavages every day for a period of four weeks, respective-ly. After obtaining blood samples (at the end of each week), differential white blood cell (WBC) counts, phagocyte activity (number), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphates (ALP) albumin and globulins levels of sam-ples were obtained. The malondialdehyde (MDA) and glutathione (GSH) levels in the se-rum were determined only in day 28 of study. The radical scavenger activity (RSA) of the RC extract was measured spectrophotometrically. Results the gamma globulin level, neu-trophil and monocyte counts and phagocyte activity increased significantly in comparison with the normal saline group. ALT, AST and ALP had not significantly differences in compared to control group. RC extract significantly increased thiobarbituric acid reactive substances (TBARS) and also decreased GSH levels in comparing to control group in day 28. Conclusion the data suggest that the RC extract has been used in traditional medicine might have immunomodulatory effects. PMID:23678431

  1. Hematological and Plasma Biochemical Parameters of Aquarium-Maintained Cownose Rays

    Microsoft Academic Search

    Claudia M. Ferreira; Cara L. Field; Allison D. Tuttle

    2010-01-01

    There is generally a dearth of information involving reference ranges of health variables for numerous elasmobranch species commonly housed in zoos and aquaria; thus, extrapolation from a few existing elasmobranch studies is commonly used to assess health parameters in these species. The primary objective of this study was to establish baseline complete blood cell count and plasma chemistry reference ranges

  2. Clinical and biochemical parameters in relation to serum leptin levels in South Indian children and adolescents

    Microsoft Academic Search

    Srinivasa Nageswara Rao; G. Prema Gurumurthy; Priya Gururajan; Sarasa Barathi Arumugam; Saibabu; V. Kirthivasan; K. M. Cherian

    2010-01-01

    Objective  To investigate the relationship between serum leptin and lipid profile in South Indian School children and adolescents, and\\u000a to evaluate the role of serum leptin in obese, overweight and congenital heart diseased children and adolescents; in South\\u000a Indian population and its correlation with anthropometric and biochemical parameters.\\u000a \\u000a \\u000a \\u000a \\u000a Methods  The study included 185 school going children and adolescents. (52 obese, 49 overweight,

  3. Effects of Lichenic Extracts (Hypogymnia physodes, Ramalina polymorpha and Usnea florida) on Human Blood Cells: Cytogenetic and Biochemical Study.

    PubMed

    Türkez, Hasan; Ayd?n, Elanur; Aslan, Ali

    2012-01-01

    Several lichen species have been used for medicinal purposes throughout the ages, and they were reported to be effective in the treatment of different disorders including tuberculosis, hemorrhoids, ulcer, dysentery and cancer. It is revealed that they may be easily accessible sources of natural drugs that could be used as a possible food supplement or in pharmaceutical industry after their safety evaluations. However, so far, the nature and/or biological roles of plenty of lichenes have not been elucidated exactly. The aim of this study was to investigate the genetic and oxidative effects of water extracts of three different lichen species; Hypogymnia physodes, Ramalina polymorpha and Usnea florida in cultured human blood cells (n = 5) for the first time. All lichen species were collected from the Erzurum and Artvin provinces (in Turkey) during August 2010. The lichen extracts were added into culture tubes at various concentrations (0 to 2000 mg/L). Chromosome aberrations (CA) and micronucleus (MN) tests were used for genotoxic influences estimation. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative stress (TOS)) were examined to determine oxidative effects. In our in-vitro test systems, it was observed that all tested lichen extracts had no mutagenic effects on human lymphocytes. Furthermore, these extracts exhibited antioxidant properties due to the type of lichen species added to the cultures. In conclusion, these lichens can be a new resource of therapeutics as recognized in this study with their non-mutagenic and antioxidant features. PMID:24250516

  4. Effects of Lichenic Extracts (Hypogymnia physodes, Ramalina polymorpha and Usnea florida) on Human Blood Cells: Cytogenetic and Biochemical Study

    PubMed Central

    Türkez, Hasan; Ayd?n, Elanur; Aslan, Ali

    2012-01-01

    Several lichen species have been used for medicinal purposes throughout the ages, and they were reported to be effective in the treatment of different disorders including tuberculosis, hemorrhoids, ulcer, dysentery and cancer. It is revealed that they may be easily accessible sources of natural drugs that could be used as a possible food supplement or in pharmaceutical industry after their safety evaluations. However, so far, the nature and/or biological roles of plenty of lichenes have not been elucidated exactly. The aim of this study was to investigate the genetic and oxidative effects of water extracts of three different lichen species; Hypogymnia physodes, Ramalina polymorpha and Usnea florida in cultured human blood cells (n = 5) for the first time. All lichen species were collected from the Erzurum and Artvin provinces (in Turkey) during August 2010. The lichen extracts were added into culture tubes at various concentrations (0 to 2000 mg/L). Chromosome aberrations (CA) and micronucleus (MN) tests were used for genotoxic influences estimation. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative stress (TOS)) were examined to determine oxidative effects. In our in-vitro test systems, it was observed that all tested lichen extracts had no mutagenic effects on human lymphocytes. Furthermore, these extracts exhibited antioxidant properties due to the type of lichen species added to the cultures. In conclusion, these lichens can be a new resource of therapeutics as recognized in this study with their non-mutagenic and antioxidant features. PMID:24250516

  5. Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab

    PubMed Central

    Zulfiqar, Samreen; Shahnawaz, Sadia; Ali, Muhammad; Bhutta, Arif Mahmood; Iqbal, Shahid; Hayat, Sikandar; Qadir, Shazia; Latif, Muhammad; Kiran, Nazia; Saeed, Ali; Ali, Muhammad; Iqbal, Furhan

    2012-01-01

    Objective To determine the presence of Babesia bovis (B. bovis) in large ruminants in southern Punjab and its effect on hematological and serum biochemical profile of host animals. Methods Blood samples were collected from 144 large ruminants, including 105 cattle and 39 buffaloes, from six districts in southern Punjab including Multan, Layyah, Muzaffar Garh, Bhakar, Bahawalnagar and Vehari. Data on the characteristics of animals and herds were collected through questionnaires. Different blood (hemoglobin, glucose) and serum (ALT, AST, LDH, cholesterol) parameters of calves and cattle were measured and compared between parasite positive and negative samples to demonstrate the effect of B. bovis on the blood and serological profile of infected animals. Results 27 out of 144 animals, from 5 out of 6 sampling districts, produced the 541-bp fragment specific for B. bovis. Age of animals (P=0.02), presence of ticks on animals (P=0.04) and presence of ticks on dogs associated with herds (P=0.5) were among the major risk factors involved in the spread of bovine babesiosis in the study area. ALT concentrations were the only serum biochemical values that significantly varied between parasite positive and negative cattle. Conclusions : This study has reported for the first time the presence of B. bovis in large ruminant and the results can lead to the prevention of babesiosis in the region to increase the livestock output. PMID:23569878

  6. Effects of temperature on cellular and biochemical parameters in the crab Carcinus aestuarii (Crustacea, Decapoda).

    PubMed

    Matozzo, Valerio; Gallo, Chiara; Marin, Maria Gabriella

    2011-06-01

    The effects of temperature on cellular and biochemical parameters of the crab Carcinus aestuarii were evaluated. Crabs were kept for 7 days at 4, 17 (reference value) and 30 °C (salinity of 35 psu), and total haemocyte count (THC), haemocyte volume, haemocyte proliferation, phenoloxidase (PO) activity in both haemocyte lysate (HL) and cell-free haemolymph (CFH), CFH total protein and glucose levels, superoxide dismutase (SOD) and catalase (CAT) activities in both gills and digestive gland were evaluated. The lowest and the highest temperature significantly decreased THC, whereas haemocyte volume and haemolymph glucose concentration did not differ significantly among experimental conditions. Haemolymph protein concentration significantly reduced in crabs maintained at 30 °C, when compared with that of animals kept at 4 and 17 °C. Haemocyte proliferation increased significantly in crabs kept at 4 and 30 °C, when compared with that of crabs held at 17 °C. Likewise, a significantly higher PO activity was recorded in CFH from crabs kept at 4 and 30 °C, than in control crabs. Conversely, PO activity did not vary significantly in HL. With regard to antioxidant enzyme activities, a significant decrease in CAT activity was observed in gills from crabs kept at 4 °C, when compared to that of crabs kept at 17 °C and 30 °C. Results obtained demonstrated that the highest and lowest temperature tested influenced crab biological responses, and indicated that C. aestuarii modulated its cellular and biochemical parameters (mainly haemocyte proliferation, CFH protein concentrations and CFH PO activity) in order to cope with temperature. PMID:21546077

  7. Identification of regulatory structure and kinetic parameters of biochemical networks via mixed-integer dynamic optimization

    PubMed Central

    2013-01-01

    Background Recovering the network topology and associated kinetic parameter values from time-series data are central topics in systems biology. Nevertheless, methods that simultaneously do both are few and lack generality. Results Here, we present a rigorous approach for simultaneously estimating the parameters and regulatory topology of biochemical networks from time-series data. The parameter estimation task is formulated as a mixed-integer dynamic optimization problem with: (i) binary variables, used to model the existence of regulatory interactions and kinetic effects of metabolites in the network processes; and (ii) continuous variables, denoting metabolites concentrations and kinetic parameters values. The approach simultaneously optimizes the Akaike criterion, which captures the trade-off between complexity (measured by the number of parameters), and accuracy of the fitting. This simultaneous optimization mitigates a possible overfitting that could result from addition of spurious regulatory interactions. Conclusion The capabilities of our approach were tested in one benchmark problem. Our algorithm is able to identify a set of plausible network topologies with their associated parameters. PMID:24176044

  8. Could selenium administration alleviate the disturbances of blood parameters caused by lithium administration in rats?

    PubMed

    Kie?czykowska, Ma?gorzata; Kocot, Joanna; Kurzepa, Jacek; Lewandowska, Anna; ?elazowska, Renata; Musik, Irena

    2014-06-01

    Lithium is widely used in medicine, but its administration can cause numerous side effects. The present study aimed at the evaluation of the possible application of selenium, an essential and antioxidant element, as a protective agent against lithium toxicity. The experiment was performed on four groups of Wistar rats: I (control)-treated with saline, II (Li)-treated with lithium (Li2CO3), III (Se)-treated with selenium (Na2SeO3) and IV (Li?+?Se)-treated with lithium and selenium (Li2CO3 and Na2SeO3) in the form of water solutions by stomach tube for 6 weeks. The following biochemical parameters were measured: concentrations of sodium, potassium, calcium, magnesium, phosphorus, iron, urea, creatinine, cholesterol, glucose, total protein and albumin and activities of alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase in serum as well as whole blood superoxide dismutase and glutathione peroxidase. Morphological parameters such as red blood cells, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelets, white blood cells, neutrophils as well as lymphocytes were determined. Lithium significantly increased serum calcium and glucose (2.65?±?0.17 vs. 2.43?±?0.11; 162?±?31 vs. 121?±?14, respectively), whereas magnesium and albumin were decreased (1.05?±?0.08 vs. 1.21?±?0.15; 3.85.?±?0.12 vs. 4.02?±?0.08, respectively). Selenium given with lithium restored these parameters to values similar to those observed in the control (Ca-2.49?±?0.08, glucose-113?±?26, Mg-1.28?±?0.09, albumin-4.07?±?0.11). Se alone or co-administered with Li significantly increased aspartate aminotransferase and glutathione peroxidase. The obtained outcomes let us suggest that the continuation of research on the application of selenium as an adjuvant in lithium therapy seems warranted. PMID:24676629

  9. Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice

    PubMed Central

    Kalliokoski, Otto; Sørensen, Dorte B; Hau, Jann; Abelson, Klas S P

    2014-01-01

    Facial vein (cheek blood) and caudal vein (tail blood) phlebotomy are two commonly used techniques for obtaining blood samples from laboratory mice, while automated blood sampling through a permanent catheter is a relatively new technique in mice. The present study compared physiological parameters, glucocorticoid dynamics as well as the behavior of mice sampled repeatedly for 24?h by cheek blood, tail blood or automated blood sampling from the carotid artery. Mice subjected to cheek blood sampling lost significantly more body weight, had elevated levels of plasma corticosterone, excreted more fecal corticosterone metabolites, and expressed more anxious behavior than did the mice of the other groups. Plasma corticosterone levels of mice subjected to tail blood sampling were also elevated, although less significantly. Mice subjected to automated blood sampling were less affected with regard to the parameters measured, and expressed less anxious behavior. We conclude that repeated blood sampling by automated blood sampling and from the tail vein is less stressful than cheek blood sampling. The choice between automated blood sampling and tail blood sampling should be based on the study requirements, the resources of the laboratory and skills of the staff. PMID:24958546

  10. The effect of the combination of acids and tannin in diet on the performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs

    PubMed Central

    2010-01-01

    Background The abolition of in-feed antibiotics or chemotherapeutics as growth promoters have stimulated the swine industry to look for alternatives such as organic acids, botanicals, probiotics and tannin. The objective of the present study was to compare the effects of a combination of acids and tannin with diet with organic acids and diet without growth promoters on the growth performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs. Tannin is more natural and cheaper but possibly with the same effectiveness as organic acids with regard to growth performance. Methods Thirty-six 7 week old grower pigs, divided into three equal groups, were used in a three week feeding trial. Group I was fed basal diet, group II basal diet with added organic acids and group III basal diet with added organic and inorganic acids and tannin. Pigs were weighed before and after feeding and observed daily. Blood was collected before and after the feeding trial for the determination of selected biochemical, haematological and antioxidant enzyme parameters. One-way ANOVA was used to assess any diet related changes of all the parameters. Paired t-test was used to evaluate changes of blood parameters individually in each group of growers before and after feeding. Results No clinical health problems related to diet were noted during the three week feeding trial. The average daily gain (ADG) and selected blood parameters were not affected by the addition to basal diet of either acids and tannin or of organic acids alone. Selected blood parameters remained within the reference range before and after the feeding trial, with the exception of total serum proteins that were below the lower value of reference range at both times. The significant changes (paired t-test) observed in individual groups before and after the feeding trial are related to the growth of pigs. Conclusion Diet with acids and tannin did not improve the growth performance of grower pigs but had no deleterious effects on selected blood parameters. The possibility of beneficial effects of adding acids and tannin in diets on growth performance over a longer period, however, could not be excluded. PMID:20205921

  11. Biochemical and Hematologic Parameters for Children in the Middle Belt of Ghana

    PubMed Central

    Dosoo, David K.; Asante, Kwaku P.; Kayan, Kingsley; Adu-Gyasi, Dennis; Osei-Kwakye, Kingsley; Mahama, Emmanuel; Danso, Samuel; Amenga-Etego, Stephen; Bilson, Philip; Koram, Kwadwo A.; Owusu-Agyei, Seth

    2014-01-01

    Reference values derived from developed countries are used in many countries in Africa for interpretation of laboratory results obtained during routine healthcare and clinical trials. Use of locally derived reference values has been recommended. The purpose of the study was to establish age- and sex-specific reference values for children in the middle belt of Ghana. Reference values were determined for 21 biochemical and 18 hematologic parameters by using Clinical and Laboratory Standards Institute C28-A3 guidelines in a sample of 1,442 healthy children. Hemoglobin, hematocrit, mean cell volume, erythrocytes, urea, and creatinine were lower when compared with values from northern countries but alanine aminotransferase, aspartate aminotransferase, and total bilirubin were higher. A panel of locally relevant age- and sex-specific reference values was established for commonly used biochemical and hematologic tests in children in the middle part of Ghana. This will help in interpretation of laboratory results for clinical management of patients, screening, and safety monitoring during clinical trials. PMID:24591437

  12. The Effect of Vitamin A Supplementation on Biochemical Parameters in Multiple Sclerosis Patients

    PubMed Central

    Jafarirad, Sima; Siassi, Fereydoon; Harirchian, Mohammad-Hossein; Amani, Reza; Bitarafan, Sama; Saboor-Yaraghi, Aliakbar

    2013-01-01

    Background Vitamin A has different functions in the body and after being converted to acid form; it can play many roles in immune system regulation. Therefore, this vitamin can be used as a supplement in the treatment of diseases, such as cancer and autoimmune diseases. Vitamin A is a fat-soluble compound and its long-term consumption in high doses can have some adverse effects. Objective The current study aimed to investigate the possible complications and find solutions to minimize the adverse effects. Patients and Methods This study was a double blind randomized clinical trial. In the main study, vitamin A (as retinyl palmitate) was given to 35 multiple sclerosis (MS) patients in order to regulate their immune system with a dose of 25000 IU/day for a period of six months. To investigate the possible biochemical complications, lipid profiles, fasting blood sugar (FBS), liver enzymes, and C-reactive protein (CRP) were tested. Results Vitamin A did not have a significant difference in lipid profiles, FBS and liver enzymes between the two groups receiving vitamin A and the placebo, but CRP increased in patients who were taking vitamin A, 1.65±0.43 (mg/L) and 2.88±0.67, (Mean±SEM), before and after the intervention respectively (P=0.029), and statistical analysis showed significant differences with the group receiving placebo (P=0.011) and CRP level in vitamin A group was 1.3 mg/L more than those of the placebo group after intervention (P=0.011). Conclusions Considering that no significant difference was found in the proven vitamin A side effects, due to the increase in CRP, frequent clinical and biochemical controls are required along with vitamin A supplementation. PMID:23983997

  13. Effect of yoga therapy on reaction time, biochemical parameters and wellness score of peri and post-menopausal diabetic patients

    PubMed Central

    Madanmohan; Bhavanani, Ananda Balayogi; Dayanidy, G; Sanjay, Zeena; Basavaraddi, Ishwar V

    2012-01-01

    Background: Yogic practices may aid in the prevention and management of diabetes mellitus (DM) and reduce cardiovascular complications in the population. The present study has been undertaken to evaluate the effect of yoga therapy on reaction time, biochemical parameters and wellness score of peri and post-menopausal diabetic patients. Materials and Methods: 15 peri and post-menopausal patients receiving standard medical treatment for type 2 DM were recruited and reaction time and biochemical investigations were done before and after a comprehensive yoga therapy program comprising of three times a week sessions for six weeks. A post-intervention, retrospective wellness questionnaire compiled by ACYTER was used to evaluate the comparative feelings of the patients after the therapy program. Results: Yoga training reduced auditory reaction time (ART) from right as well as left hand, the decrease being statistically significant (P<0.05) for ART from the right hand. There was a significant (P<0.01) decrease in fasting and postprandial blood glucose levels as well as low density lipoprotein. The decrease in total cholesterol, triglycerides, and very low density lipoprotein and increase in high density lipoprotein was also statistically significant (P<0.05). All the lipid ratios showed desirable improvement with a decrease (P<0.01) of TC/HDL and LDL/HDL ratios and increase (P<0.05) in the HDL/LDL ratio. Discussion or Conclusion: Shortening of RT implies an improvement in the information processing and reflexes and is the first such report in diabetic patients. This has clinical significance and is worth further exploration with wider, well controlled, randomized studies in the diabetic population. Changes in blood glucose levels may be due to improved insulin sensitivity, decline in insulin resistance and increased sensitivity of the pancreatic b cells to glucose signals. Yoga improved the ‘heart friendly’ status of lipid profile in our subjects and as our participants were peri and post-menopausal, the decrease in cardiovascular risk profile is of greater significance. A comprehensive yoga therapy program has the potential to enhance the beneficial effects of standard medical management of diabetes mellitus and can be used as an effective complementary or integrative therapy program. PMID:22346060

  14. Monitoring of various types of immunotherapy with gramineal pollens. I. Variation of clinical and biochemical parameters.

    PubMed

    Miguel Lozano, R; Guerra Pasadas, F; Arenas Vacas, A; Daza Muñoz, J C; Torres Murillo, P; Sánchez Guijo, P

    1992-01-01

    We assessed the clinical effectiveness and adverse effect of various types of immunotherapy on 42 pollinotic patients by monitoring their evolution on administration of Perennial lolium specific extract standardized in protein nitrogen units to 14 of them, the same extract but standardized in biological units (BUs) to another 13, and an extract containing four different pollens standardized in BUs to the remaining 15. For this purpose we measured clinical and medicinal scores, liver and kidney biochemical parameters, skin reactions and circulating immunocomplexes (CICs), both before immunotherapy was started (basal levels) and after 3, 6 and 12 month's treatment. We found no significant differences in clinical effectiveness or the occurrence of any adverse effects on administration of the different immunotherapies after one year's monitoring. We did found gradually decreased reactions to the skin tests, which thus provide a reliable means of monitoring allergen-specific immunotherapy. PMID:1296464

  15. Characterization of blood biochemical markers during aging in the Grey Mouse Lemur (Microcebus murinus): impact of gender and season

    PubMed Central

    2012-01-01

    Background Hematologic and biochemical data are needed to characterize the health status of animal populations over time to determine the habitat quality and captivity conditions. Blood components and the chemical entities that they transport change predominantly with sex and age. The aim of this study was to utilize blood chemistry monitoring to establish the reference levels in a small prosimian primate, the Grey Mouse Lemur (Microcebus murinus). Method In the captive colony, mouse lemurs may live 10–12 years, and three age groups for both males and females were studied: young (1–3 years), middle-aged (4–5 years) and old (6–10 years). Blood biochemical markers were measured using the VetScan Comprehensive Diagnostic Profile. Because many life history traits of this primate are highly dependent on the photoperiod (body mass and reproduction), the effect of season was also assessed. Results The main effect of age was observed in blood markers of renal functions such as creatinine, which was higher among females. Additionally, blood urea nitrogen significantly increased with age and is potentially linked to chronic renal insufficiency, which has been described in captive mouse lemurs. The results demonstrated significant effects related to season, especially in blood protein levels and glucose rates; these effects were observed regardless of gender or age and were likely due to seasonal variations in food intake, which is very marked in this species. Conclusion These results were highly similar with those obtained in other primate species and can serve as references for future research of the Grey Mouse Lemur. PMID:23131178

  16. Computing fluxes and chemical potential distributions in biochemical networks: energy balance analysis of the human red blood cell

    E-print Network

    De Martino, Daniele; De Martino, Andrea; Marinari, Enzo

    2011-01-01

    The analysis of non-equilibrium steady states of biochemical reaction networks relies on finding the configurations of fluxes and chemical potentials satisfying stoichiometric (mass balance) and thermodynamic (energy balance) constraints. Efficient methods to explore such states are crucial to predict reaction directionality, calculate physiologic ranges of variability, estimate correlations, and reconstruct the overall energy balance of the network from the underlying molecular processes. While different techniques for sampling the space generated by mass balance constraints are currently available, thermodynamics is generically harder to incorporate. Here we introduce a method to sample the free energy landscape of a reaction network at steady state. In its most general form, it allows to calculate distributions of fluxes and concentrations starting from trial functions that may contain prior biochemical information. We apply our method to the human red blood cell's metabolic network, whose space of mass-ba...

  17. Daily and seasonal variations of the biochemical blood indices in the lower apes

    Microsoft Academic Search

    Yu. P. Butnev

    1963-01-01

    Experiments were carried out on male and female hamadryl baboons daily throughout a 24-hour period. The blood sugar and blood ATP fell to a low level at 8–9:00 p.m. Concerning seasonal variation, we showed that the blood ATP and cholestrol blood contents increased during the cold season, whereas the sugar level dropped. The residual nitrogen, inorganic and acid-soluble phosphorus, and

  18. Effect of vitamin e supplementation on biochemical parameters in pesticides sprayers of grape gardens of Western maharashtra (India).

    PubMed

    Patil, Jyotsna A; Patil, Arun J; Sontakke, Ajit V; Govindwar, Sanjay P

    2012-04-01

    The aim of this study was to see the biochemical effects of pesticides on sprayers of grape gardens before and after 15 days of vitamin E supplementations in Western Maharashtra (India), who were occupationally exposed to various pesticides over a long period of time (about 5 to 15 years). Blood samples were collected from all study group subjects for biochemical parameters assays before and after 15 days of vitamin E supplementation. Sprayers of grape gardens were given 400 mg of vitamin E tablet/day for 15 days. After 15 days of vitamin E supplementation to sprayers of grape gardens, we observed significantly decreased aspartate transaminase (10.88 %, P < 0.05, r = 0.88), alanine transaminase (25.92 %, P < 0.01, r = 0.46) and total proteins (3.32 %, P < 0.01, r = 0.33), whereas, no statistically significant change was found in serum acetyl cholinesterase, C-reactive proteins, albumin (ALB), globulins and ALB/globulin ratio as compared to before vitamin E supplementation. Sprayers of grape gardens, who received vitamin E supplementation, showed significantly decreased serum lipid peroxide (LP) (18.75 %, P < 0.001, r = 0.63) and significantly increased RBC-superoxide dismutase (SOD) (12.88 %, P < 0.001, r = 0.85), RBC-Catalase (CAT) (24.49 %, P < 0.001, r = 0.70), plasma ceruloplasmin (CP) (4.6 %, P < 0.01, r = 0.80), serum zinc (4.57 %, P < 0.01, r = 0.83) and serum copper (4.37 %, P < 0.01, r = 0.79) as compared to values before vitamin E supplementation. These results showed that vitamin E supplementation has ameliorating effects on these transaminase enzymes, suggesting that it may have a protective effect on liver, from pesticides induced damage. In this study vitamin E supplementation might have decreased LP levels by breaking chain reaction of lipid peroxidation. Present results indicate that vitamin E plays a crucial role in restoring the antioxidant enzymes such as SOD, CAT and CP, in population exposed to pesticides. This helps to enhance its antioxidant ability. Therefore, it is suggested that farmers, pesticide applicators, workers in the pesticide industry and other pesticide users, who come in regular contact with pesticides, may be benefited by supplementation with vitamin E. PMID:23543683

  19. A simple and feasible questionnaire to estimate menstrual blood loss: relationship with hematological and gynecological parameters in young women

    PubMed Central

    2014-01-01

    Background Menstrual blood loss (MBL) has been shown to be an important determinant in iron status, work performance and well-being. Several methods have been developed to estimate MBL, the standard quantitative method however has limited application in clinical practice as it is expensive and requires women to collect, store and submit their sanitary products for analysis. We therefore aimed to develop a MBL-score based on a questionnaire, and to validate it by several hematological and biochemical parameters in women of childbearing age. Methods A total of 165 healthy young women were recruited. Hematological (hematocrit, hemoglobin, erythrocyte, leucocyte and platelet counts) and iron status (serum iron, serum ferritin, serum transferrin, and total iron binding capacity) parameters were analyzed at baseline. Women were asked to fulfill two gynecological questionnaires: a general questionnaire, to inform about the volunteer’s general menstrual characteristics; and a MBL questionnaire, to provide details of the duration of menstruation, number of heavy blood loss days, and number and type of pads and/or tampons used during the heaviest bleeding day, for all consecutive menstrual periods during 16 weeks. A MBL-score was calculated for each period and women, and its reliability determined by the Cronbach’s alpha coefficient. Pearson’s linear correlation tests were performed between blood parameters and the MBL-score. Two clusters were formed according the MBL-score (cluster 1: low MBL and cluster 2: high MBL). Results Significant higher MBL-score was observed in women who reported having a history of anemia (p?=?0.015), staining the bed at night during menstruation (p?blood loss (p?=?0.044), compared to those who did not. Women who used hormonal contraceptives presented lower MBL-scores than the others (p?=?0.004). The MBL-score was negatively associated with log-ferritin (p?=?0.006) and platelet count (p?=?0.011). Women in cluster 1 presented higher ferritin (p?=?0.043) than women in cluster 2. Conclusions We developed an easy and practical method for estimating menstrual blood loss based on a score calculated from a questionnaire in healthy women at childbearing age. The MBL-score is highly reliable and reflects menstrual blood loss validated by hematological and biochemical parameters. PMID:24886470

  20. Determining tumor blood flow parameters from dynamic image measurements

    NASA Astrophysics Data System (ADS)

    Libertini, Jessica M.

    2008-11-01

    Many recent cancer treatments focus on preventing angiogenesis, the process by which a tumor promotes the growth of large and efficient capillary beds for the increased nourishment required to support the tumor's rapid growth[l]. To measure the efficacy of these treatments in a timely fashion, there is an interest in using data from dynamic sequences of contrast-enhanced medical imaging, such as MRI and CT, to measure blood flow parameters such as perfusion, permeability-surface-area product, and the relative volumes of the plasma and extracellular-extravascular space. Starting with a two compartment model presented by the radiology community[2], this work challenges the application of a simplification to this problem, which was originally developed to model capillary reuptake[3]. While the primary result of this work is the demonstration of the inaccuracy of this simplification, the remainder of the paper is dedicated to presenting alternative methods for calculating the perfusion and plasma volume coefficients. These methods are applied to model data sets based on real patient data, and preliminary results are presented.

  1. Study of a number of biochemical indices of the blood and tissue of dogs after prolonged gamma-radiation

    NASA Technical Reports Server (NTRS)

    Alers, I.; Alersova, E.; Praslichka, T.; Mishurova, E.; Sedlakova, A.; Malatova, Z.; Akhunov, A. A.; Markelov, B. A.

    1974-01-01

    The glucose content in blood and the lipid content in serum and tissues of dogs exposed to chronic radiation for 3 and 5 years were studied. In tissues of these animals, the concentration of soluble DNA and DNA contained in DNP was studied in the spleen, lymph node (deep cervical node) and bone marrow of thigh bones. Results indicate that chronic gamma irradiation significantly changes concentrations of glucose in the blood, and that of several lipids in serum and tissues. A reduction in the concentration of DNP in tested organs reflects changes in the relative number of cells with various nuclear cytoplasmic ratios; most pronounced changes in biochemical indices occur in dogs exposed to chronic gamma radiation in doses of 125 rad per year.

  2. Correlation between biochemical parameters and susceptibility of freshwater fish to malathion.

    PubMed

    Li Shao-Non; Fan De-Fang

    1996-07-01

    Acute toxicity (96-h LC50) of malathion was tested using five species of freshwater fish, namely, topmouth gudgeon (Pseudorasbora parva), goldfish (Carassius auratus), nile tilapia (Tilapia nilotica), mosquitofish (Gambusia affinis), and rainbow trout (Salmo gairdneri). Correlation was found between susceptibility and biochemical parameters such as activity of brain acetylcholinesterase (AChE) and in vitro resistance of the enzyme to inhibition (IC50) of malaoxon (a major metabolite of malathion). The in vitro study also showed that malaoxon instead of malathion was the main inhibitor of AChE. Susceptibility to malathion was considerably changed as the fish was pretreated with piperonyl butoxide (PB, a P-450 inhibitor) and triphenyl phosphate (TPP, an inhibitor of carboxylesterase), respectively. Toxicity of malathion was significantly increased by TPP, but the responses of fish to PB were quite different among species. This suggested that both carboxylesterase and monooxygenase played an important role in susceptibility determination, and great variations existed among species in activity of monooxygenase. PMID:8691510

  3. Clinical, hematological and biochemical parameters of dairy cows experimentally infected with Vaccinia virus.

    PubMed

    Rehfeld, Izabelle S; Guedes, Maria Isabel M C; Matos, Ana Carolina D; de Oliveira, Tércia M L; Rivetti, Anselmo V; Moura, Ana Carolina J; Paes, Paulo Ricardo O; do Lago, Luiz Alberto; Kroon, Erna G; Lobato, Zélia Inês P

    2013-10-01

    Vaccinia virus (VACV) is the etiological agent of bovine vaccinia (BV), an important zoonosis that affects dairy cattle. There are many aspects of the disease that remain unknown, and aiming to answer some of these questions, the clinical, hematological, and biochemical parameters of VACV experimentally infected cows were evaluated. In the first part of the study, lactating cows were infected with VACV-GP2 strain. In the second part, animals previously infected with VACV-GP2 were divided into two treatment groups: Group 1, immunosuppressed cows; and Group 2, re-infected cows. In this study, BV could be experimentally reproduced, with similar lesions as observed in natural infections. Moreover, a short incubation period and local lymphadenopathy were also observed. VACV could be detected by PCR and isolated from scabs taken from teat lesions of all inoculated and re-inoculated animals. Lymphocytosis and neutrophilia were observed in all animals from the first part of the experiment, and lymphopenia and relative neutrophilia were observed in the immunosuppressed animals. Detection of viral DNA in oral mucosa lesions suggests that viral reactivation might occur in immunosuppressed animals. Moreover, clinical disease with teat lesions may occur in previously VACV-infected cows under the experimental conditions of the present study. PMID:23747141

  4. Effects of methanolic extract of Moringa oleifera leaves on semen and biochemical parameters in cryptorchid rats.

    PubMed

    Afolabi, Ayobami Oladele; Aderoju, Hameed Adeola; Alagbonsi, Isiaka Abdullateef

    2013-01-01

    While anti-oxidant effects of Moringa oleifera in much oxidative stress related diseases have been well reported, cryptorchidism on the other hand has been shown to cause oxidative stress. However, study is scanty on the likely role of Moringa oleifera in reducing cryptorchidism-induced oxidative stress in rats has not been studied. The present study looked into the effects of methanolic extract of Moringa oleifera leaves (MEMO) on semen and biochemical parameters in cryptorchid rats. Twenty male albino rats (200-250 g) were randomly divided into 4 groups (n=5 each). Groups A and B were sham-operated and treated with corn-oil and 200 mg/kg of MEMO respectively, while groups C and D were rendered cryptorchid and also treated with corn-oil and 200 mg/kg of MEMO respectively. Cryptorchid rats had lower testicular weight, sperm count, germ cell count, testicular superoxide dismutase (SOD) concentration, testicular total protein and higher testicular malondialdehyde (MDA) concentration compared to sham-operated rats. MEMO had no significant effect on testicular weight and MDA concentration, while it significantly increased sperm count, germ cell count, testicular SOD and total protein in the cryptorchid rats. The present study suggests that MEMO ameliorates cryptorchidism associated germ cell loss and oxidative stress. PMID:24311830

  5. Correlation Analysis between Complex Relative Permittivity and Biochemical Components for Blood of Dialysis Patients before and after Hemodialysis

    NASA Astrophysics Data System (ADS)

    Takeda, Akira; Takata, Kazuyuki; Nagao, Hirotomo; Wang, Jianqing; Fujiwara, Osamu

    We previously measured for healthy subjects and patients who require hemodialysis the complex relative permittivity (?r´-j?r´´) of whole blood, and found that ?r´-axis intercept ?rt´ of straight line approximation to the Cole-Cole plots at frequencies from 200 MHz to 1GHz is significantly different with a level of less than 1% among its averaged values for healthy subjects and patients before and after dialysis, though any correlations between ?rt´ and blood urea nitrogen (BUN) and creatinine being used as main indices for hemodialysis evaluation have not so far been clarified. In this study, to make correlation analyses between the intercept ?rt´ and blood biochemical components for nine patients before and after hemodialysis, we measured six kinds of their blood components including BUN and creatinine, and calculated their Pearson product-moment correlation coefficients for ?rt´ along with significant probability P based on a t-test. It should be noted that P is a reference probability to determine whether or not a null hypothesis can be rejected, and that the P value of 0.05 is commonly used as a significance level for statistical test. As a result, we found that a strong correlation with P < 0.05 is observed between ?rt´ and albumin, while there are not always significant correlations with P > 0.05 between ?rt´ and other blood components including BUN and creatinine. Although albumin is not used as an index for hemodialysis efficiency, it can reflect water amount in blood vessels and liver operation. This finding implies that ?rt´ could be used as an index for evaluating blood viscosity and liver function.

  6. Correlation of seizures and biochemical parameters of oxidative stress in experimentally induced inflammatory rat models.

    PubMed

    Rao, Ramya S; Medhi, Bikash; Khanduja, Krishan Lal; Pandhi, Promila

    2010-06-01

    The role of oxidative stress in the pathogenesis of various conditions including epilepsy, inflammatory bowel disease and rheumatoid arthritis is evolving. The aim of this study was to find out the correlation between various inflammatory models with seizures and antioxidant parameters. Fifty-four male rats were divided into three groups of colitis, adjuvant arthritis and cotton wool granuloma (CWG). Each group had three subgroups of control, model and treatment. Thalidomide was used as treatment in colitis and arthritis group, whereas etoricoxib was used in CWG group. In colitis and arthritis groups, thalidomide was administered for 3 and 17 days, respectively, whereas etoricoxib was administered for 7 days in CWG group. At the end of treatment protocols, a subconvulsive dose of pentylenetetrazole (PTZ) (40 mg/kg i.p.) was injected intraperitoneally to note seizure onset and score. After confirming the presence of inflammation by morphological and histological studies, plasma and brain biochemical parameters of oxidative stress were estimated. The models of colitis, arthritis and CWG were effectively produced as evidenced by morphological scores (P < 0.001). Thalidomide reduced the morphological score (P < 0.002) and seizure grade (P < 0.001), whereas increased seizure onset (P < 0.001) in the arthritis group. There was an increase in malondialdehyde levels in the brain of thalidomide-treated groups (P < 0.002) and a significant decrease in superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels. There was neither improvement in seizure nor any significant changes in lipid peroxidation and antioxidant enzyme levels in etoricoxib-treated group. Thalidomide was effective in reducing the extent of arthritis as well as reducing the seizure scoring and increasing seizure onset in the adjuvant arthritis group. As it increased lipid peroxidation and reduced SOD and GPx, further evaluation is necessary with respect to oxidative stress. PMID:20584211

  7. Duration of Voluntary Breath Holding and Blood Parameters

    Microsoft Academic Search

    L. I. Irzhak; P. V. Polyakov

    2002-01-01

    Stange's and Genci's functional tests performed at rest and during exercise and blood tests have shown that the duration of voluntary breath holding depends on appearance in the blood of hypoxia, hypercapnia, and acidosis signs only after the influence of fatigue of respiratory muscles and respiratory center has become insufficient.

  8. Dynamics of cerebral blood flow regulation explained using a lumped parameter model

    E-print Network

    Olufsen, Mette Sofie

    Dynamics of cerebral blood flow regulation explained using a lumped parameter model METTE S October 2001 Olufsen, Mette S., Ali Nadim, and Lewis A. Lipsitz. Dynamics of cerebral blood flow: R611­R622, 2002; 10.1152/ajpregu. 00285.2001.--The dynamic cerebral blood flow response to sudden

  9. Genetic and phenotypic parameters of egg production and some constituents of blood serum in Fayoumi layers

    E-print Network

    Boyer, Edmond

    Genetic and phenotypic parameters of egg production and some constituents of blood serum in Fayoumi (i) egg production beside the following blood serum constituents: (2) inorganic phosphorus, (3 the daughters of 40 sires and 241 dams. Blood serum analyses were done using the Sequential Multiple Auto

  10. Automatic retinal blood vessel parameter calculation in spectral domain optical coherence tomography

    Microsoft Academic Search

    Hassan Wehbe; Marco Ruggeri; Shuliang Jiao; Giovanni Gregori; Carmen A. Puliafito

    2007-01-01

    Measurement of retinal blood vessel parameters like the blood blow in the vessels may have significant impact on the study and diagnosis of glaucoma, a leading blinding disease worldwide. Optical coherence tomography (OCT) is a noninvasive imaging technique that can provide not only microscopic structural imaging of the retina but also functional information like the blood flow velocity in the

  11. An Enzyme Cascade in the Blood Clotting Mechanism, and its Function as a Biochemical Amplifier

    Microsoft Academic Search

    R. G. Macfarlane

    1964-01-01

    AFTER years of confusion, it seems that a relatively simple pattern is emerging from present theories of blood coagulation. Its recognition is assisted by the Roman numeral terminology of the International Committee on Blood Clotting Factors, which, by displacing a profusion of synonyms, allows the basis of factual agreement to be seen. Physiological clotting seems to be initiated by contact

  12. PREDICTING RODENT CARCINOGENICITY OF HALOGENATED HYDROCARBON BY IN VIVO BIOCHEMICAL PARAMETERS

    EPA Science Inventory

    Forty halogenated hydrocarbons of known rodent carcinogenicity (24 carcinogens, 16 noncarcinogens), including many promoters of carcinogenesis, nongenotoxic carcinogens and hepatocarcinogens were selected for study. he effects of these 40 chemicals on four biochemical assays (hep...

  13. Dietary Quality Indices and Biochemical Parameters Among Patients With Non Alcoholic Fatty Liver Disease (NAFLD)

    PubMed Central

    Hashemi kani, Ali; Alavian, Seyed Moayed; Esmaillzadeh, Ahmad; Adibi, Peyman; Azadbakht, Leila

    2013-01-01

    Background Dietary intake might have important role in non-alcoholic fatty liver diseases (NAFLD). Although, there are some reports on dietary intake and anthropometrics measurements, few studies have focused on the markers of assessing whole diet like dietary quality indices. Objectives Therefore, our aim was to determine the diet quality indices and biochemical parameters among patients with NAFLD and healthy individuals. Patients and Methods This case-control study was performed on 100 patients with NAFLD and 100 healthy subjects who were attending to Gastrointestinal Research Center, Baqiyatallah University, Tehran, Iran during the recent years. Usual dietary intake was assessed by three dietary records (one weekend and two week days). Healthy eating index (HEI), dietary diversity score (DDS), dietary energy density (DED), mean adequacy ratio of nutrients (MAR) were assessed according to the standard methods. Results Patients with NAFLD had higher body mass index, weight and waist circumference compared to the healthy group (P < 0.05). Serum levels of liver enzymes, triglyceride, LDL, BUN, and uric acid were higher in patients with NAFLD (P < 0.05). Although patients with NAFLD had higher energy, carbohydrate and fat intake, their values for antioxidant vitamins, calcium and vitamin D were lower than healthy subjects (P < 0.05). HEI and MAR were higher among healthy group, and DED was lower among them. Nutrient adequacy ratio for calcium, vitamin D, and antioxidant micronutrients were lower in patients with NAFLD (P < 0.05). Conclusions It seems that dietary quality indices may be associated with NAFLD. Calcium, vitamin D, and antioxidant micronutrients intake might be lower among patients with NAFLD based on this case-control study. Further prospective studies should be conducted in this regard. PMID:24065998

  14. [Effect of starfish (Asterias amurensis) intake on weight gain and blood biochemical values in rats].

    PubMed

    Hasegawa, Mayumi; Yamazaki, Kaoru; Baba, Osamu

    2013-01-01

    We observed the effect of starfish (Asterias amurensis) intake during 67 days, on rats, in terms of the activity of enzymes related to liver function and biochemical values related to weight gain, lipid metabolism and safety. 1. Starfish (Asterias amurensis) did not induce a significant difference of body weight change. 2. Starfish (Asterias amurensis) intake did not affect organ weight. 3. Starfish (Asterias amurensis) intake did not affect lipid metabolism, liver function, or protein nutrition in this experiment. PMID:24190291

  15. Identification of optical parameters of the whole blood depending on its oxygenation level

    Microsoft Academic Search

    Alexandre J. Douplik; Victor B. Loschenov

    1996-01-01

    This work is the optical study of interinfluence between different physiological parameters of the human blood such as oxygenation rate and erythrocyte aggregation level. The evaluation of such influence upon optical properties of the blood has been also carried out in vitro. Optical parameters such as total and diaphragm transmission and diffuse reflection have been investigated by in vitro set

  16. Alterations in Biochemical Parameters During Subacute Toxicity of Fluoride Alone and in Conjunction with Aluminum Sulfate in Goats

    Microsoft Academic Search

    Vinay Kant; Anil Kumar Srivastava; Pawan Kumar Verma; Rajinder Raina

    2009-01-01

    Fluoride toxicity is a serious health problem in many parts of the globe. In present study, sodium fluoride at 20 mg\\/kg alone\\u000a and in conjunction with aluminum sulfate at 150 mg\\/kg was administered orally daily for 30 days in healthy goats of group\\u000a 1 and 2, respectively, to access the alterations in the various biochemical parameters during subacute toxicity of fluoride\\u000a alone and

  17. Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41.

    PubMed

    Taechalertpaisarn, Tana; Crosnier, Cecile; Bartholdson, S Josefin; Hodder, Anthony N; Thompson, Jenny; Bustamante, Leyla Y; Wilson, Danny W; Sanders, Paul R; Wright, Gavin J; Rayner, Julian C; Cowman, Alan F; Gilson, Paul R; Crabb, Brendan S

    2012-01-01

    The genomes of Plasmodium parasites that cause malaria in humans, other primates, birds, and rodents all encode multiple 6-cys proteins. Distinct 6-cys protein family members reside on the surface at each extracellular life cycle stage and those on the surface of liver infective and sexual stages have been shown to play important roles in hepatocyte growth and fertilization respectively. However, 6-cys proteins associated with the blood-stage forms of the parasite have no known function. Here we investigate the biochemical nature and function of two blood-stage 6-cys proteins in Plasmodium falciparum, the most pathogenic species to afflict humans. We show that native P12 and P41 form a stable heterodimer on the infective merozoite surface and are secreted following invasion, but could find no evidence that this complex mediates erythrocyte-receptor binding. That P12 and P41 do not appear to have a major role as adhesins to erythrocyte receptors was supported by the observation that antisera to these proteins did not substantially inhibit erythrocyte invasion. To investigate other functional roles for these proteins their genes were successfully disrupted in P. falciparum, however P12 and P41 knockout parasites grew at normal rates in vitro and displayed no other obvious phenotypic changes. It now appears likely that these blood-stage 6-cys proteins operate as a pair and play redundant roles either in erythrocyte invasion or in host-immune interactions. PMID:22848665

  18. Biochemical characterization of blood plasma of coronary artery disease patients by in vitro high-resolution proton NMR spectroscopy.

    PubMed

    Malik, Anu; Sharma, Uma; Lakshmy, R; Narang, Rajiv; Jagannathan, Naranamanglam R

    2015-03-01

    This study aimed to investigate the biochemical profile of blood plasma of patients with coronary artery disease (CAD) and angiographically normal subjects (controls) to determine biomarkers for their differentiation. In this double blind study, 5 mL venous blood was drawn before angiography from CAD patients (n=60) and controls (n=13) comprising angiography normal individuals. In vitro high-resolution NMR spectroscopy of these blood plasma samples was carried out at 400 MHz, and intensity data were analysed with partial least square discriminant analysis. Categorization of subjects as controls or CAD patients and the patients further as single vessel disease (SVD), double vessel disease (DVD) and triple vessel disease (TVD) was done at the end of the study based on their angiography reports. Raised levels of lipids, alanine (Ala) and isoleucine/leucine/valine (Ile/Leu/Val) were observed in CAD patients compared with controls. Partial least square discriminant analysis showed separation between controls vs CAD patients. TVD patients showed increased levels of Ile/Leu/Val and Ala compared with controls and SVD. Alanine, Ile/Leu/Val, and LDL/VLDL appear as possible biomarkers for distinguishing between controls and patients with SVD and TVD. A metabolic adaptation of myocardium may play a role in raising the Ala level. PMID:25740139

  19. Action of Brazilian propolis on hematological and serum biochemical parameters of Blue-fronted Amazons (Amazona aestiva, Linnaeus, 1758) in captivity.

    PubMed

    Silva, Cínthia R B; Putarov, Thaila C; Fruhvald, Erika; Destro, Flavia C; Marques Filho, Wolff C; Thomazini, Camila M; Barbosa, Tatiana S; Orsi, Ricardo O; Siqueira, Edson R

    2014-07-01

    The present study aimed to evaluate the effect of propolis use on hematological and serum biochemical parameters in Blue-fronted Amazons (Amazona aestiva). For this, 12 adult birds were distributed randomly into individual cages, divided into treatments with different propolis levels (A = 0.0%; B = 0.5%; and C = 1.0%), in 3 distinct phases (I, II, and III), with 15-d duration for phases I and III and 30 d for phase II, totaling 60 d. In phases I and III, all birds received treatment A ration, and in phase II received A, B, or C (4 birds per treatment). At the end of each phase, blood was collected for biochemical and hematological evaluations. The variables were analyzed by ANOVA (P < 0.05). Results suggest that 0.5% propolis reduced lactate dehydrogenase levels, whereas treatment B augmented hemoglobin concentrations and eosinophil count. It is concluded that 0.5% propolis improves levels of lactate dehydrogenase, hemoglobin, and eosinophils. PMID:24864289

  20. A study of the noncompliance of blood banks on safety and quality parameters in blood donation camps in Bengaluru

    PubMed Central

    Agarwal, Rajat Kumar; Dhanya, Rakesh; Parmar, Lalith G.; Vaish, Arpit; Sedai, Amit; Periyavan, Sundar

    2015-01-01

    Aims: The compliance of safety and quality parameters laid out by national and international guidelines in outdoor blood donation camps has not been studied in India. Our study aimed at identifying, monitoring, analyzing, and developing preventive strategies for several key parameters associated with the quality and safety of outdoor voluntary blood donation camps (VBDC). Settings: The study covered a total of 424 VBDCs at various locations in Bengaluru, Karnataka (South India) from 2009 to 2013. Seven government hospitals based blood banks, three private hospitals based blood banks and two voluntary standalone blood banks participated in the VBDCs included in the study. Materials and Methods: At the onset, the quality and safety standards to be followed were discussed and agreed upon. During the study, noncompliance (NC) to the agreed upon standards were recorded and shared. Periodic trainings were also organized to help minimize NC. Results: One or more instances of NC in 73% of the VBDCs. Highest NC were observed associated with punctuality (34%), wearing gloves (16%), hemoglobin (Hb) estimation (11%) and donor screening and selection other than Hb check (8-9%). Conclusion: For all 16 parameters under study, significant NC was observed. As a whole private hospital based blood banks were more noncompliant. The high degree of NC to matters relating to quality and safety in VBDCs is high and warrants for urgent attention and further study. Our study also shows that regular monitoring and systematic and strategic intervention can decrease the rate of NC. PMID:25722568

  1. Evaluation of tropical water sources and mollusks in southern Brazil using microbiological, biochemical, and chemical parameters.

    PubMed

    Souza, Doris Sobral Marques; Ramos, Ana Paula Dores; Nunes, Fabrício Flores; Moresco, Vanessa; Taniguchi, Satie; Leal, Diego Averaldo Guiguet; Sasaki, Silvio Tarou; Bícego, Márcia Caruso; Montone, Rosalinda Carmela; Durigan, Maurício; Teixeira, Adriano Luiz; Pilotto, Mariana Rangel; Delfino, Nicésio; Franco, Regina Maura Bueno; Melo, Cláudio Manoel Rodrigues de; Bainy, Afonso Celso Dias; Barardi, Célia Regina Monte

    2012-02-01

    Florianópolis, a city located in the Santa Catarina State in southern Brazil, is the national leading producer of bivalve mollusks. The quality of bivalve mollusks is closely related to the sanitary conditions of surrounding waters where they are cultivated. Presently, cultivation areas receive large amounts of effluents derived mainly from treated and non-treated domestic, rural, and urban sewage. This contributes to the contamination of mollusks with trace metals, pesticides, other organic compounds, and human pathogens such as viruses, bacteria, and protozoan. The aim of this study was to perform a thorough diagnosis of the shellfish growing areas in Florianópolis, on the coast of Santa Catarina. The contamination levels of seawater, sediments, and oysters were evaluated for their microbiological, biochemical, and chemical parameters at five sea sites in Florianópolis, namely three regular oyster cultivation areas (Sites 1, 2, and oyster supplier), a polluted site (Site 3), and a heavily polluted site (Site 4). Samples were evaluated at day zero and after 14 days. Seawater and sediment samples were collected just once, at the end of the experiment. Antioxidant defenses, which may occur in contaminated environments in response to the increased production of reactive oxygen species (ROS) by organisms, were analyzed in oysters, as well as organic compounds (in oysters and sediment samples) and microbiological contamination (in oysters and seawater samples). The results showed the presence of the following contaminants: fecal coliforms in seawater samples (four sites), human adenovirus (all sites), human noroviruses GI and GII (two sites), Hepatitis A viruses (one site), JC Polyomavirus in an oyster sample from the oyster supplier, Giardia duodenalis cysts, and Cryptosporidium sp oocysts (one site). Among organochlorine pesticides, only DDT (dichlorodiphenyltrichloroethane) and HCH (hexachlorocyclohexane) were detected in some sediment and oysters samples in very low levels; site 4 had the highest concentrations of total aliphatic hydrocarbons, PAHs, and linear alkylbenzenes (LABs) found either in oysters or in sediment samples. The major concentration of fecal sterol coprostanol was found at site 4, followed by site 3. After 14 days of allocation in the four selected sites, there was a significant difference in the enzymes analyzed at the monitored spots. The detection of different contaminants in oysters, seawater, and sediment samples in the present study shows the impact untreated or inadequately treated effluents have on coastal areas. These results highlight the need for public investment in adequate wastewater treatment and adequate treatment of oysters, ensuring safe areas for shellfish production as well as healthier bivalve mollusks for consumption. PMID:22036209

  2. Effect of storage on biochemical and microbiological parameters of edible truffle species

    Microsoft Academic Search

    Roberta Saltarelli; Paola Ceccaroli; Paola Cesari; Elena Barbieri; Vilberto Stocchi

    2008-01-01

    The effects of different storage treatments on the most common edible truffle species, such as Tuber magnatum and Tuber borchii (white truffles), Tuber melanosporum and Tuber aestivum (black truffles), were analysed. Biochemical and microbiological profiles were monitored, in order to evaluate possible alterations during truffle preservation. After harvesting, some fresh samples were kept at 4°C for 30days, other samples were

  3. PREDICTING RODENT CARCINOGENICITY OF AMES TEST "FALSE POSITIVES" BY IN VIVO BIOCHEMICAL PARAMETERS

    EPA Science Inventory

    Twenty-eight chemicals known to be mutagenic in the Ames test but not carcinogenic in rodent bioassays were selected for study. The effects of these chemicals on four biochemical assays (hepatic DNA damage by alkaline elution (DD), hepatic ornithine decarboxylase activity (ODC), ...

  4. BIOCHEMICAL RESPONSES TO FIBROPAPILLOMA AND CAPTIVITY IN THE GREEN TURTLE

    Microsoft Academic Search

    J. Y. Swimmer

    2000-01-01

    Blood biochemical parameters were compared for green turtles (Chelonia mydas) with and without green turtle fibropapillomatosis (GTFP) from both captive and wild populations in Hawaii (USA) and from a captive population from California (USA), during the period between 1994 and 1996. Statistical analysis did not detect an influence of disease in any of the blood parameters for free-ranging turtles; however,

  5. BIOCHEMICAL CHANGES IN HUMANS UPON EXPOSURE TO NITROGEN DIOXIDE WHILE AT REST

    EPA Science Inventory

    The biochemical response to controlled exposures of nitrogen dioxide (NO2) was studied in 19 human subjects exposed to 0.2 ppm NO2 for 2 hr and compared to 15 control subjects exposed to filtered air for 2 hr. Seven biochemical blood parameters, including glutathione, red blood c...

  6. The effect of cigarette smoke exposure on vitamin D level and biochemical parameters of mothers and neonates

    PubMed Central

    2013-01-01

    Background Exposure to cigarette smoke during pregnancy leads to several adverse effects on mother and child. The purpose of this study was to evaluate the effect of being a passive smoker during pregnancy on vitamin D level and related biochemical indices including parathyroid hormone, calcium, phosphorus and alkaline phosphatase in mothers and newborns. Methods One hundred eight pregnant women and their newborns participated in a historical cohort study in two equal groups (n?=?54) with and without cigarette smoke exposure. Maternal blood and urine samples and blood samples of umbilical cord were obtained in the delivery room. Concentration of 25-hydroxy vitamin D and related biochemical indices in samples of maternal and cord blood were investigated. Exposure to cigarette smoke was evaluated through questionnaire and maternal urine and umbilical cord serum cotinine levels. Results The mean level of 25-hydroxyvitamin D in maternal serum was 9.28?±?5.19?ng/mlin exposed and 10.75?±?5.26?ng/ml in non-exposed group(p?>?0.05). The mean concentration of 25-hydroxy vitamin D in cord serum was 10.83?±?6.68?ng/ml in the exposed and 11.05?±?4.99?ng/ml in the non-exposed group(p?>?0.05). The exposed mothers had significantly higher parathyroid hormone level (p?=?0.013), lower serum calcium (p?=?0.024) and higher serum alkaline phosphatase (p?=?0.024). There was a significant correlation between maternal and umbilical cord serum 25-hydroxyvitamin D within both exposed and non-exposed groups (p?

  7. Effects of cadmium at sub-lethal concentration on growth and biochemical parameters in rainbow trout (Oncorhynchus mykiss)

    PubMed Central

    2013-01-01

    Cadmium (Cd), as one of heavy metals and an environmental stressor, may alter many physiological processes like growth and serum parameters in fish. The main objective of this study was to determine the effects of cadmium at sub-lethal concentrations (1 and 3 ?g/l) on growth and serum biochemical parameters including enzymes, i.e. alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), glucose, triglyceride, cholesterol and total protein in rainbow trout (Oncorhynchus mykiss). Trout were exposed to cadmium, and, at intervals of 1, 15, and 30 days, selected parameters were evaluated. Condition Factor (K), Specific Growth Rate (SGR) and Body Weight Gain (BWG) consistently decreased, while Food Conversion Ratio (FCR) increased at the end of experiment. Glucose was elevated in trout exposed to both Cd concentrations at day 15 and then returned to levels comparable to control fish. Triglyceride and cholesterol decreased transiently at day 15 and then increased at day 30. Total protein, AST, ALT and ALP increased linearly by time and Cd concentration. This investigation suggests that growth and serum biochemical parameters could be used as important and sensitive biomarkers in ecotoxicological studies concerning the effects of metal contamination and fish health. PMID:23782857

  8. Effects of dermal exposure to chlorpyrifos on liver and brain structures and biochemical parameters in rabbits

    Microsoft Academic Search

    Amirali Solati; Abbas Tavasoly; Mohammad Kazem Koohi; Seid Hosein Marjanmehr; Babak Rezvanjoo

    Chlorpyrifos, an organophosphate insecticide, inhibits the enzyme acetylcholinesterse resulting in the accumulation of acetylcholine\\u000a at the nerve endings. In the present research chlorpyrifos was administered via dermal contact to New Zealand white rabbits\\u000a to determine its effects on biochemical indices on the rabbits as well as the pathological changes in their liver and brain.\\u000a Twelve adult male rabbits were randomly

  9. Effects of subchronic exposure to meloxicam on some hematological, biochemical and liver histopathological parameters in rats

    Microsoft Academic Search

    F. M. K. Al-Rekabi; D. A. Abbas; N. R. Hadi

    This experiment was conducted to study the effects of sub chronic exposure of Meloxicam in male albino rats by measuring Hematological, Biochemical and Histopathological changes of Livers in (18) rats divided equally into two treatment groups, T1 which dosed with 0.2 mg\\/KG.BW as therapeutic dose and T2 which dosed with 0.6 mg\\/KG.BW as three fold dose, while the other (6)

  10. Comparing Transcription Rate and mRNA Abundance as Parameters for Biochemical Pathway and Network Analysis

    Microsoft Academic Search

    Brewster Hayles; Sailu Yellaboina; Degeng Wang

    2010-01-01

    The cells adapt to extra- and intra-cellular signals by dynamic orchestration of activities of pathways in the biochemical networks. Dynamic control of the gene expression process represents a major mechanism for pathway activity regulation. Gene expression has thus been routinely measured, most frequently at steady-state mRNA abundance level using micro-array technology. The results are widely used in statistical inference of

  11. Automatic retinal blood vessel parameter calculation in spectral domain optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Wehbe, Hassan; Ruggeri, Marco; Jiao, Shuliang; Gregori, Giovanni; Puliafito, Carmen A.

    2007-02-01

    Measurement of retinal blood vessel parameters like the blood blow in the vessels may have significant impact on the study and diagnosis of glaucoma, a leading blinding disease worldwide. Optical coherence tomography (OCT) is a noninvasive imaging technique that can provide not only microscopic structural imaging of the retina but also functional information like the blood flow velocity in the retina. The aim of this study is to automatically extract the parameters of retinal blood vessels like the 3D orientation, the vessel diameters, as well as the corresponding absolute blood flow velocity in the vessel. The parameters were extracted from circular OCT scans around the optic disc. By removing the surface reflection through simple segmentation of the circular OCT scans a blood vessel shadowgram can be generated. The lateral coordinates and the diameter of each blood vessel are extracted from the shadowgram through a series of signal processing. Upon determination of the lateral position and the vessel diameter, the coordinate in the depth direction of each blood vessel is calculated in combination with the Doppler information for the vessel. The extraction of the vessel coordinates and diameter makes it possible to calculate the orientation of the vessel in reference to the direction of the incident sample light, which in turn can be used to calculate the absolute blood flow velocity and the flow rate.

  12. Effect of hafnium and titanium coated implants on several blood biochemical markers after osteosynthesis in rabbits

    PubMed Central

    Yousef, Ashraf; Akhtyamov, Ildar; Shakirova, Faina; Zubairova, Lyaili; Gatina, Elmira; Aliev, ?lchin

    2014-01-01

    Purpose: An experimental study comparing the dynamics of several biochemical markers before and after osteosynthesis, utilizing implants coated with titanium and hafnium nitrides and non-coated implants on rabbits’ bones. Materials and methods: The Study has been conducted on 30 rabbits of both sexes, at the age of 6-7 months, weighing 2526.5±74.4 gm. Animals underwent open osteotomy of the tibia in the middle third of the diaphysis followed by the intramedullary nailing. The level of alkaline phosphatase, calcium, phosphorus, total protein, glucose, ALT and AST were monitored for 60 days. Results: the use of implants coated with titanium and hafnium nitrides, which have high strength, thermal and chemical stability, was not accompanied by the development of additional negative reactive changes compared to non-coated implants. Conclusion: Nanotechnology used in manufacturing bioinert coatings for implants for osteosynthesis, has made the post-operative period less complicated as reflected by less expressed changing in the markers of bone metabolism and hepatotoxicity. PMID:25419385

  13. Correlation between glucose concentrations in serum, plasma, and whole blood measured by a point-of-care glucometer and serum glucose concentration measured by an automated biochemical analyzer for canine and feline blood samples.

    PubMed

    Tauk, Barbara S; Drobatz, Kenneth J; Wallace, Koranda A; Hess, Rebecka S

    2015-06-15

    Objective-To investigate the correlation between glucose concentrations in serum, plasma, and whole blood measured by a point-of-care glucometer (POCG) and serum glucose concentration measured by a biochemical analyzer. Design-Prospective clinical study. Samples-96 blood samples from 80 dogs and 90 blood samples from 65 cats. Procedures-Serum, plasma, and whole blood were obtained from each blood sample. The glucose concentrations in serum, plasma, and whole blood measured by a POCG were compared with the serum glucose concentration measured by a biochemical analyzer by use of the Lin concordance correlation coefficient (?c) and Bland-Altman plots. Results-For both canine and feline samples, glucose concentrations in serum and plasma measured by the POCG were more strongly correlated with the serum glucose concentration measured by the biochemical analyzer (?c, 0.98 for both canine serum and plasma; ?c, 0.99 for both feline serum and plasma) than was that in whole blood (?c, 0.62 for canine samples; ?c, 0.90 for feline samples). The mean difference between the glucose concentrations determined by the biochemical analyzer and the POCG in serum, plasma, and whole blood was 0.4, 0.3, and 31 mg/dL, respectively, for canine samples and 7, 6, and 32 mg/dL, respectively, for feline samples. Conclusions and Clinical Relevance-Results indicated that use of a POCG to measure glucose concentrations in serum or plasma may increase the accuracy and reliability of diagnostic and treatment decisions associated with glucose homeostasis disorders in dogs and cats. (J Am Vet Med Assoc 2015;246:1327-1333). PMID:26043130

  14. Effects of selected enzyme inhibitors on blood and eggshell parameters in the laying hen 

    E-print Network

    Long, Jody Renee

    1987-01-01

    EFFECTS OF SELECTED ENZYME INHIBITORS ON BLOOD AND EGGSHELL PARAMETERS IN THE LAYING HEN A Thesis by JODY RENEE LONG Submitted to the Graduate College of Texas ASM University in partial fullfilment of the requirement for the degree... of Committee) @q ~ y. g~p( A. Naqi (Member) C. R. cger (Head of De rtment) C. R. cger (Hember) December 1987 ABSTRACT Effects of Selected Enzyme Inhibitors on Blood and Eggshell Parameters in the Laying Hen. (December, 1987) Jody R. Long, B. S...

  15. Changes in quality and biochemical parameters in 'Idared' apples during prolonged shelf life and 1-MCP treatment.

    PubMed

    Bizjak, Jan; Slatnar, Ana; Stampar, Franci; Veberic, Robert

    2012-12-01

    In this study, changes in quality and various biochemical parameters of 'Idared' apples during prolonged shelf life period after ultra-low oxygen (ULO) storage were investigated. Additionally, the impact of the postharvest application of 1-methylcyclopropene (1-MCP) on different parameters was evaluated. After the harvest, apples were stored in the ULO storage for 6 months and then exposed to room temperature. Fruit firmness, peel color, and changes in sugars, organic acids and phenolics were monitored during the 3 weeks of shelf life. Malic acid, sugars and firmness decreased at room temperature. However, the color of the apples remained unchanged. The level of citric and ascorbic acid remained constant. Levels of phenolics in the peel increased significantly, whereas remained constant in the pulp of apples. 1-MCP treatment resulted in higher amounts of fructose and glucose, malic acid and greater firmness of apples. However, 1-MCP did not influence the phenolic content, ascorbic acid or color. The results obtained indicate that the content of different health-promoting compounds of apples does not change dramatically at room temperature. At the same time these results suggest that 1-MCP could be useful for maintaining certain quality and biochemical parameters and might extend the shelf life of apples. PMID:23175783

  16. Effects of chitin and sepia ink hybrid hemostatic sponge on the blood parameters of mice.

    PubMed

    Zhang, Wei; Sun, Yu-Lin; Chen, Dao-Hai

    2014-04-01

    Chitin and sepia ink hybrid hemostatic sponge (CTSH sponge), a new biomedical material, was extensively studied for its bene?cial biological properties of hemostasis and stimulation of healing. However, studies examining the safety of CTSH sponge in the blood system are lacking. This experiment aimed to examine whether CTSH sponge has negative effect on blood systems of mice, which were treated with a dosage of CTSH sponge (135 mg/kg) through a laparotomy. CTSH sponge was implanted into the abdominal subcutaneous and a laparotomy was used for blood sampling from abdominal aortic. Several kinds of blood parameters were detected at different time points, which were reflected by coagulation parameters including thrombin time (TT), prothrombin time (PT), activated partial thromboplatin time (APTT), fibrinogen (FIB) and platelet factor 4 (PF4); anticoagulation parameter including antithrombin III (AT-III); fibrinolytic parameters including plasminogen (PLG), fibrin degradation product (FDP) and D-dimer; hemorheology parameters including blood viscosity (BV) and plasma viscosity (PV). Results showed that CTSH sponge has no significant effect on the blood parameters of mice. The data suggested that CTSH sponge can be applied in the ?eld of biomedical materials and has potential possibility to be developed into clinical drugs of hemostatic agents. PMID:24727395

  17. Biochemical parameters in the blood of Holstein calves given immunoglobulin Y-supplemented colostrums

    PubMed Central

    2014-01-01

    Background In any calf rearing system it is desirable to obtain healthy animals, and reduce morbidity, mortality, and economic losses. Bovine syndesmochorial placentation prevents the direct transfer of bovine immunoglobulins to the fetus, and calves are born hypogammaglobulinemic. These calves therefore require colostrum immediately after birth. Colostrum is rich in immunoglobulins (Ig) and its consumption results in the transfer of passive immunity to calves. The Ig absorption occurs within the first 12 h after birth. Immunoglobulin Y (IgY), derived from chicken egg yolk, has been used in the prevention and control of diseases affecting calves because it is very similar in structure and function to immunoglobulin G (IgG). In the current study, we sought to establish whether administration routes of colostrum supplemented with avian IgY affected passive immunity in calves. Results No significant differences were observed with respect to route of administration for colostrum. However, we did observe some differences in certain interactions between the various treatments. Calves fed colostrum containing egg yolk had higher levels of TP, ALB, and IgG, along with increased GGT activity. Conclusions Our results suggest that supplementing colostrum with egg yolk has a beneficial effect when given to calves, regardless of administration route. PMID:25022282

  18. Identification of optical parameters of the whole blood depending on its oxygenation level

    NASA Astrophysics Data System (ADS)

    Douplik, Alexandre Y.; Loschenov, Victor B.

    1996-05-01

    This work is the optical study of interinfluence between different physiological parameters of the human blood such as oxygenation rate and erythrocyte aggregation level. The evaluation of such influence upon optical properties of the blood has been also carried out in vitro. Optical parameters such as total and diaphragm transmission and diffuse reflection have been investigated by in vitro set up for the whole blood. Change of the blood oxygenation has been created by membrane oxygenator. According to the received data the coefficients of scattering and absorption and the mean cosine of scattering have been calculated for the whole blood by inverse Monte-Carlo simulations for following wavelengths: 633, 675, 820, 860 nm.

  19. Relationships between blood lactate parameters and endurance performance

    NASA Astrophysics Data System (ADS)

    Papadopoulos, Charilaos

    The purpose of this study was to determine if one lactate threshold definition, as determined from an incremental maximal exercise test, correlates the best with the average speed of running during two different distances (10 km and 21.1 km). Thirteen well-trained runners (age: 30.9 +/- 2.2 yrs; height: 179.9 +/- 1.2 cm; weight: 70.5 +/- 1.8 kg; body fat: 8.5 +/- 1.0%; V?O2max: 65.3 +/- 1.0 ml·kg -1·min-1) completed an incremental maximal exercise test to determine their individual lactate profile, one 10 km and one half marathon time trial (TT) on a treadmill. During these trials, oxygen consumption, heart rate, and blood responses were measured. Blood samples were collected through a venous catheter placed in an antecubital vein to determine lactate responses. Correlation coefficients between the running velocity at the different LT definitions and the average running speed during each time trial were calculated using Pearson's correlation coefficient. A dependent t-test was used to determine statistical difference for the mean lactate response between the two running distances and a two-way ANOVA was used to determine the difference between trials over time. All the lactate threshold definitions were significantly correlated (r ? 0.59; p < 0.05) with both distances. The LTlog was the definition with the highest correlation for both 10 km time trial (r = 0.926) and the half marathon (r = 0.906). The mean lactate concentration during the 10 km time trial (3.52 +/- 0.4 mmol/L) was significantly higher than the mean lactate concentration during the half marathon (1.86 +/- 0.3 mmol/L). These results indicate that any point on the lactate profile curve correlates with running performance with LTlog providing the highest correlation. However, the LT log was not the best indicator for each individual for each distance, indicating that there is a need to consider individual differences.

  20. Impact of iron overload on interleukin-10 levels, biochemical parameters and oxidative stress in patients with sickle cell anemia

    PubMed Central

    Barbosa, Maritza Cavalcante; dos Santos, Talyta Ellen Jesus; de Souza, Geane Félix; de Assis, Lívia Coêlho; Freitas, Max Victor Carioca; Gonçalves, Romélia Pinheiro

    2013-01-01

    Objective The aim of this study was to evaluate the impact of iron overload on the profile of interleukin-10 levels, biochemical parameters and oxidative stress in sickle cell anemia patients. Methods A cross-sectional study was performed of 30 patients with molecular diagnosis of sickle cell anemia. Patients were stratified into two groups, according to the presence of iron overload: Iron overload (n = 15) and Non-iron overload (n = 15). Biochemical analyses were performed utilizing the Wiener CM 200 automatic analyzer. The interleukin-10 level was measured by capture ELISA using the BD OptEIAT commercial kit. Oxidative stress parameters were determined by spectrophotometry. Statistical analysis was performed using GraphPad Prism software (version 5.0) and statistical significance was established for p-values < 0.05 in all analyses. Results Biochemical analysis revealed significant elevations in the levels of uric acid, triglycerides, very low-density lipoprotein (VLDL), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), urea and creatinine in the Iron overload Group compared to the Non-iron overload Group and significant decreases in the high-density lipoprotein (HDL) and low-density lipoprotein (LDL). Ferritin levels correlated positively with uric acid concentrations (p-value < 0.05). The Iron overload Group showed lower interleukin-10 levels and catalase activity and higher nitrite and malondialdehyde levels compared with the Non-iron overload Group. Conclusion The results of this study are important to develop further consistent studies that evaluate the effect of iron overload on the inflammatory profile and oxidative stress of patients with sickle cell anemia. PMID:23580881

  1. Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows

    PubMed Central

    Rasolomboahanginjatovo, Hasina Santatriniaina; Dupras, Raynald; Mills, Louis; Lefebvre, Réjean

    2014-01-01

    The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at day 7 of the estrous cycle to start the SOV protocol, as well as on the day of embryo recovery (DER). The SOV group produced 7.5 ± 6.7 oocytes/embryos, of which 3.4 ± 4.7 were TE. Serum urea and E2 and uterine Glu, CK, LDH, TP, P4 and PGFM in the control group and serum P4 and PGFM and uterine LDH and PGFM in the SOV group were significantly higher (p < 0.01) at DER than day 7. At DER, uterine urea, LDH, PGFM and TP and serum urea, LDH, PGFM, and P4 concentrations were higher (p < 0.01) in the SOV group than the control. There was no significant variation in uterine bacteriology or cytology. Overall, these results infer that SOV affects both serum profile and uterine secretions, and that these changes may influence the number of TE. PMID:24675828

  2. Weighted next reaction method and parameter selection for efficient simulation of rare events in biochemical reaction systems

    PubMed Central

    2011-01-01

    The weighted stochastic simulation algorithm (wSSA) recently developed by Kuwahara and Mura and the refined wSSA proposed by Gillespie et al. based on the importance sampling technique open the door for efficient estimation of the probability of rare events in biochemical reaction systems. In this paper, we first apply the importance sampling technique to the next reaction method (NRM) of the stochastic simulation algorithm and develop a weighted NRM (wNRM). We then develop a systematic method for selecting the values of importance sampling parameters, which can be applied to both the wSSA and the wNRM. Numerical results demonstrate that our parameter selection method can substantially improve the performance of the wSSA and the wNRM in terms of simulation efficiency and accuracy. PMID:21910924

  3. Effects of Dietary L-carnitine Supplementation on Growth Performance, Organ Weight, Biochemical Parameters and Ascites Susceptibility in Broilers Reared Under Low-temperature Environment

    PubMed Central

    Wang, Y. W.; Ning, D.; Peng, Y. Z.; Guo, Y. M.

    2013-01-01

    The objective of this study was to investigate the effects of L-carnitine on growth performance, organ weight, biochemical parameters of blood, heart and liver, and ascites susceptibility of broilers at different ages reared under a low-temperature environment. A total of 420 1-d-old male Ross 308 broilers were randomly assigned to two dietary treatments with fifteen replicates of fourteen broilers each. Treatment diets consisted of L-carnitine supplementation at levels of 0 and 100 mg/kg. At 11-d of age, low temperature stress was used to increase ascites susceptibility. Blood, heart and liver samples were collected at different ages for analysis of boichemical parameters. The results showed that, there was no significant difference in growth performance with L-carnitine supplementation, but the mortality due to ascites was significantly decreased. Dietary L-carnitine supplementation significantly reduced heart index (HI) and ascites heart index (AHI) on d 21, lung index (LUI) on d 35 and liver index (LI) on d 42. The broilers fed diets containing L-carnitine had significantly lower red blood cell counts (RBC), hemoglobin (HGB) concentration and hematocrit (HCT) on d 42. Dietary L-carnitine supplementation significantly reduced malondialdehyde (MDA) content of heart tissue on d 21 and 35, and significantly increased total superoxide dismutase (T-SOD) and Glutathione peroxidase (GSH-Px) activity of the heart on d 21 and 42. L-carnitine supplementation significantly reduced serum triglyceride (TG) content on d 28 and 35 and serum glucose (GLU) on d 35 and 42, and significantly increased serum total protein (TP) and globulin (GLO) content on d 42. L-carnitine supplementation significantly enhanced liver succinodehydrogenase (SDH), malic dehydrogenase (MDH) and Na+-K+-ATPase activity on d 28, and tended to reduce the lactic acid (LD) level of liver on d 35 (p = 0.06). L-carnitine supplementation significantly reduced serum uric acid (UA) content on d 28, 35 and 42. Based on the current results, it can be concluded that dietary L-carnitine supplementation reduced organ index, red blood cell counts and hematocrit, enhanced antioxidative capacity of the heart, enhanced liver enzymes activity involved in tricarboxylic acid cycle, and reduced serum glucose and triglyceride. Therefore, it is suggested that L-carnitine can potentially reduce susceptibility and mortality due to ascites. PMID:25049781

  4. Effects of depletion sampling by standard three-pass pulsed DC electrofishing on blood chemistry parameters of fishes from Appalachian streams

    USGS Publications Warehouse

    Densmore, Christine L.; Panek, Frank M.

    2013-01-01

    Adverse effects on fishes captured by electrofishing techniques have long been recognized, although the extent of associated physical injury and behavioral alterations are highly variable and dependent on a number of factors. We examined the effects of three-pass pulsed DC (PDC) electrofishing on two salmonid species (Rainbow Trout Oncorhynchus mykiss and Brook Trout Salvelinus fontinalis) and five other genera (Green Sunfish Lepomis cyanellus, Potomac Sculpin Cottus girardi, Fathead Minnow Pimephales promelas, Largemouth Bass Micropterus salmoides, and Channel Catfish Ictalurus punctatus) common to Appalachian streams. We examined the corresponding effects of PDC electroshock on the following physiological indicators of stress and trauma: blood glucose and serum lactate, as well as on other blood chemistry, namely, enzymes, electrolytes, minerals, and proteins. All species demonstrated physiological responses to PDC electroshock, indicated by the biochemical differences in blood parameters in unshocked and shocked groups of fish with or without gross evidence of hemorrhagic trauma. Serum lactate was the most consistent indicator of these effects. Significant differences in whole blood glucose levels were also noted in treatment groups in all species except Green Sunfish, although the patterns observed were not as consistent as for serum lactate. Elevations in the serum enzymes, aspartate aminotransferase and creatine kinase, in the electroshocked fish occurred only in the two salmonid species. In many instances, although blood parameters were elevated in electroshocked fish compared with the unshocked controls for a given species, there were no differences in those levels in electroshocked fish based on the presence of gross hemorrhagic trauma to axial musculature. While some of the blood parameters examined correlated with both the occurrence of electroshock and the resultant tissue injury, there was no apparent link between the altered blood chemistry and increased mortality 30 d after electrofishing.

  5. Early Surgical Decompression Restores Neurovascular Blood Flow and Ischemic Parameters in an in Vivo Animal Model of Nerve Compression Injury

    PubMed Central

    Jung, James; Hahn, Peter; Choi, Bernard; Mozaffar, Tahseen; Gupta, Ranjan

    2014-01-01

    Background: Chronic nerve compression neuropathies result in decreased blood flow at the site of compression. Surgical decompression of the nerve often has variable postoperative results. The current study examines whether the timing of surgical intervention is an important variable in reversing the compression-induced ischemia and associated changes in biochemical markers. Methods: An established model of chronic nerve compression injury was created in 100 C57BL/6 mice, and serial electrophysiological examinations were used to confirm the creation of a chronic nerve compression injury. Laser speckle imaging was used to measure neural blood flow. Nerves in the animals that did not undergo decompression were harvested at two, four, and six weeks after injury and analyzed for hypoxia-inducible factor 1? (HIF1?), catalase, superoxide dismutase (SOD), and matrix metalloproteinases (MMPs) 2 and 9. Surgical decompression in other animals was performed at either an early (two-week) or late (six-week) time point after injury, with specimens harvested at multiple time points after decompression. One-way analysis of variance with Bonferroni correction was performed. Results: Chronic nerve compression injury initially induced hyperemia (1.37 ± 0.50 times that in the contralateral, uninjured nerve) followed by a decline in neural blood flow by four weeks (0.66 ± 0.14, p = 0.0313). In parallel, HIF1?, catalase, and SOD were elevated early after compression, whereas extracellular matrix-altering proteins were elevated later in the disease. Although early decompression yielded a return of blood flow to a hyperemic state (1.35 ± 0.16, p = 0.0057), late decompression did not result in reversal of the abnormal neurovascular flow. With late decompression, an MMP9-mediated structural alteration of the extracellular matrix was seen, producing irreversible changes in blood flow parameters. Although nerve conduction velocity measurements returned to normal two weeks after decompression irrespective of the timing of the surgical intervention, distal latency returned to normal only after early decompression (0.97 ± 0.06 msec compared with 1.22 ± 0.06 msec for late decompression, p = 0.009). Conclusions: Chronic nerve compression injuries decreased neurovascular flow and induced ischemia by upregulating HIF1?, catalase, and MMP9. Early surgical intervention offered better return to normal electrophysiological parameters compared with late intervention. Clinical Relevance: These data present a clinical correlate to the variable functional outcomes seen following surgical release of chronic nerve compression injuries and provide early support for using distal latency as a predictor of outcomes following surgical release. PMID:24897737

  6. Early detection of crop injury from herbicide glyphosate by leaf biochemical parameter inversion

    NASA Astrophysics Data System (ADS)

    Zhao, Feng; Guo, Yiqing; Huang, Yanbo; Reddy, Krishna N.; Lee, Matthew A.; Fletcher, Reginald S.; Thomson, Steven J.

    2014-09-01

    Early detection of crop injury from herbicide glyphosate is of significant importance in crop management. In this paper, we attempt to detect glyphosate-induced crop injury by PROSPECT (leaf optical PROperty SPECTra model) inversion through leaf hyperspectral reflectance measurements for non-Glyphosate-Resistant (non-GR) soybean and non-GR cotton leaves. The PROSPECT model was inverted to retrieve chlorophyll content (Ca+b), equivalent water thickness (Cw), and leaf mass per area (Cm) from leaf hyperspectral reflectance spectra. The leaf stress conditions were then evaluated by examining the temporal variations of these biochemical constituents after glyphosate treatment. The approach was validated with greenhouse-measured datasets. Results indicated that the leaf injury caused by glyphosate treatments could be detected shortly after the spraying for both soybean and cotton by PROSPECT inversion, with Ca+b of the leaves treated with high dose solution decreasing more rapidly compared with leaves left untreated, whereas the Cw and Cm showed no obvious difference between treated and untreated leaves. For both non-GR soybean and non-GR cotton, the retrieved Ca+b values of the glyphosate treated plants from leaf hyperspectral data could be distinguished from that of the untreated plants within 48 h after the treatment, which could be employed as a useful indicator for glyphosate injury detection. These findings demonstrate the feasibility of applying the PROSPECT inversion technique for the early detection of leaf injury from glyphosate and its potential for agricultural plant status monitoring.

  7. [The influence of afobazole on biochemical and histo-patho-morphological parameters of endothelial function at experimental diabetes mellitis in rats].

    PubMed

    Dzugkoev, S G; Kozyrev, K M; gumanova, N G; Dzugkoeva, F S

    2012-01-01

    Oxidizing stress in rats with experimental diabetes mellitis is accompanied by endothelial dysfunction develops. Its biochemical markers are the increase of concentration of blood MDA, the impairments of cell antioxidant depence and a decrease in concentration of total metabolites of NO and expression of endothelial NO-synthetase (e-NOS). Biochemical changes are considered with histopathomorphologic impairments of aortic endothelium. Treatment with afobazole suppressed free-radical oxidation, increased activity of SOD and concentration of total metabolites of NO and a level of eNOS expression and also restored of a histologic pattern of aortic endothelium due to activation of nucleocytoplasmic regenerative processes. PMID:23413688

  8. Effects of gluten-free breads, with varying functional supplements, on the biochemical parameters and antioxidant status of rat serum.

    PubMed

    ?wieca, Micha?; Regu?a, Julita; Suliburska, Joanna; Z?otek, Urszula; Gawlik-Dziki, Urszula

    2015-09-01

    This paper examines the effects of gluten-free bread enriched with functional ingredients (milk powder, poppy, sunflower and pumpkin seeds, egg yolk, carum, hazel nuts and amaranth) on the morphological and biochemical parameters and antioxidant status of rats serum. Rats were provided test diets--gluten-free breads and water ad libitum. After 14 days, the animals were weighed and killed. A hazel nut-amaranth bread diet significantly increased the level of thrombocytes when compared to control bread. A mixed bread diet significantly decreased cholesterol levels in rats. All fortified breads decreased triglyceride levels and alanine transaminase activity and caused an increase in antiradical activity of the serum. In rats fed with poppy-milk bread, milk-seed bread and mixed bread, a marked decrease in superoxide dismutase activity was found. Enriched breads reduced the levels of triglyceride and improved the antiradical properties of serum, although the physiological relevance of this needs to be confirmed by human studies. PMID:25842337

  9. Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins

    PubMed Central

    Brito, Paula M.; Antunes, Fernando

    2014-01-01

    The lack of kinetic data concerning the biological effects of reactive oxygen species is slowing down the development of the field of redox signaling. Herein, we deduced and applied equations to estimate kinetic parameters from typical redox signaling experiments. H2O2-sensing mediated by the oxidation of a protein target and the switch-off of this sensor, by being converted back to its reduced form, are the two processes for which kinetic parameters are determined. The experimental data required to apply the equations deduced is the fraction of the H2O2 sensor protein in the reduced or in the oxidized state measured in intact cells or living tissues after exposure to either endogenous or added H2O2. Either non-linear fittings that do not need transformation of the experimental data or linearized plots in which deviations from the equations are easily observed can be used. The equations were shown to be valid by fitting to them virtual time courses simulated with a kinetic model. The good agreement between the kinetic parameters estimated in these fittings and those used to simulate the virtual time courses supported the accuracy of the kinetic equations deduced. Finally, equations were successfully tested with real data taken from published experiments that describe redox signaling mediated by the oxidation of two protein tyrosine phosphatases, PTP1B and SHP-2, which are two of the few H2O2-sensing proteins with known kinetic parameters. Whereas for PTP1B estimated kinetic parameters fitted in general the present knowledge, for SHP-2 results obtained suggest that reactivity toward H2O2 as well as the rate of SHP-2 regeneration back to its reduced form are higher than previously thought. In conclusion, valuable quantitative kinetic data can be estimated from typical redox signaling experiments, thus improving our understanding about the complex processes that underlie the interplay between oxidative stress and redox signaling responses. PMID:25325054

  10. Commercial formulation containing 2,4-D affects biochemical parameters and morphological indices of silver catfish exposed for 90 days.

    PubMed

    Menezes, Charlene; Fonseca, Milene B; Leitemperger, Jossiele; Pretto, Alexandra; Moraes, Bibiana S; Murussi, Camila R; Baldisserotto, Bernardo; Loro, Vania L

    2015-04-01

    The objective of this study was to verify whether a commercial formulation of 2,4 dichlorophenoxyacetic acid [2,4-D dimethylamine salt (DMA)] affects the growth and biochemical parameters of silver catfish (Rhamdia quelen) after 90 days of exposure. The fish exposed to 0.5 or 2.0 mg/L of DMA presented exhibited decreased growth parameters. Glucose was reduced in the mucus layer at both concentrations, and the total protein level was increased at the highest concentration tested. Fish exposed to DMA showed reduced liver and kidney glycogen at both concentrations tested, while in the muscle, glycogen was reduced only at 2.0 mg/L. Glucose was increased in the liver and decreased in the muscle and kidney at both concentrations and was not altered in the plasma. Lactate was increased in all the tissues and decreased in the plasma. Protein levels were reduced in the liver and plasma at both concentrations, while in the muscle, it was decreased at a concentration of 2.0 mg/L. Levels of thiobarbituric acid-reactive substances were reduced in the liver and increased in the muscle at both concentrations and did not change in the brain. DMA increased catalase activity in the liver at both concentrations tested. The present study demonstrates the effects of long-term exposure to DMA. Some parameters could be used as toxicity indicators to identify the presence of DMA in an aquatic environment. PMID:25213788

  11. Blood

    MedlinePLUS

    ... a mixture of blood cells and plasma. Continue Red Blood Cells Red blood cells (RBCs, and also ... conditions involving the blood include: Diseases of the Red Blood Cells The most common condition affecting the ...

  12. Comparison of pigeon guillemot, Cepphus columba, blood parameters from oiled and unoiled areas of Alaska eight years after the Exxon Valdez oil spill

    USGS Publications Warehouse

    Seiser, P.E.; Duffy, L.K.; McGuire, David A.; Roby, D.D.; Golet, G.H.; Litzow, M.A.

    2000-01-01

    In 1997, we compared the haematological and plasma biochemical profiles among populations of pigeon guillemots, Cepphus columba, in areas oiled and not oiled by the 1989 Exxon Valdez oil spill (EVOS) that occurred in Prince William Sound (PWS), Alaska. Pigeon guillemot populations in PWS were injured by EVOS and have not returned to pre-spill levels. If oil contamination is limiting recovery of pigeon guillemots in PWS, then we expected that blood parameters of pigeon guillemots would differ between oiled and unoiled areas and that these differences would be consistent with either toxic responses or lower fitness. We collected blood samples from chicks at approximately 20 and 30 days after hatching. Physiological changes associated with chick growth were noted in several blood parameters. We found that only calcium and mean cell volume were significantly different between the chicks in oiled and unoiled areas. Despite these differences, blood biomarkers provided little evidence of continuing oil injury to pigeon guillemot chicks, eight years after the EVOS. Preliminary data from adults indicated elevated aspartate aminotransferase activity in the adults from the oiled area, which is consistent with hepatocellular injury. Because adults have greater opportunities for exposure to residual oil than nestlings, we recommend studies that fully evaluate the health of adults residing in oiled areas. (C) 2000 Elsevier Science Ltd.

  13. Analysis of parameters affecting engraftment in children undergoing autologous peripheral blood stem cell transplants

    Microsoft Academic Search

    E Figuerres; PR Haut; M Olzewski; M Kletzel

    2000-01-01

    Eighty-three pediatric patients underwent autologous peripheral blood stem cell transplants at a single institution and were included in a study evaluating the correlations between five engraftment parameters and the time to both neutrophil and platelet recovery. The parameters included: the number of nucleated cells per kg (TNC\\/kg), the absolute CD34+ cell content per kg (CD34+\\/kg), the number of mononuclear cells

  14. Protective effect of royal jelly on fertility and biochemical parameters in bleomycin-?induced male rats

    PubMed Central

    Amirshahi, Tayebeh; Najafi, Gholamreza; Nejati, Vahid

    2014-01-01

    Background: Bleomycin (BL) is a glycopeptide antibiotic obtained from the bacterium Streptomyces verticillus which is routinely used for treatment of human cancers. Royal jelly (RJ) is a production from the hypo pharyngeal, mandibular and post cerebral glands of nurse bees. RJ consists of 66% water, 15% sugars, 5% lipids, and 13% proteins, essential amino acids and vitamins. Objective: The aim of present study was to evaluate protective effect of royal jelly on sperm parameters and malondialdehyde (MDA) production in rat. Materials and Methods: Forty adult male wistar rats (220±20gr) were randomly divided into 4 groups (n=10). Control group (CG) received normal saline 10 ml/kg twice a week with Intraperitoneal (I.P) for 48 days (0.3 ml/rat(. Royal Jelly group (RJG) received jelly (100 mg/kg daily) for 48 days orally. Bleomycin group (BLG) received BL (10 mg/kg twice a week) with I.P for 48 days. Royal Jelly+ Bleomycin group (RJ+BLG) received royal Jelly (100 mg/kg /day) orally concomitant with BL administration. Sperm count, motility, and viability were investigated and chromatin quality and DNA integrity were also analyzed. Serum testosterone and MDA concentrations were measured as well. Results: BL caused decline significantly (p<0.05) sperm count, sperm viability, motility as well as testosterone concentration compared to control group while significant (p<0.05) increases in immature sperm, sperm with damaged DNA and MDA concentration were announced in BL in comparison with CG and RJ+BLG. Royal jelly improved Bleomycin-induced toxicity on sperm parameters and testosterone and MDA concentrations. Conclusion: The present results support the idea that BL adversely affects sperm parameters and MDA and the RJ with antioxidant properties has positive effects on these parameters. This article extracted from M.Sc. thesis. (Tayebeh amirshahi) PMID:24799882

  15. Improvement of estimation parameter for frame-rate analysis of blood flow using laser speckle image sensing

    NASA Astrophysics Data System (ADS)

    Yokoi, Naomichi; Maeda, Takaaki; Shimatani, Yuichi; Funamizu, Hideki; Aizu, Yoshihisa

    2014-01-01

    Improved estimation parameters are proposed for frame-rate analysis of blood flow in skin tissue and a blood vessel using laser speckle image sensing. These parameters are introduced for visualizing a blood flow distribution with improving image quality and computation time or with reducing effects of a speckle contrast and random noises. Experiments were conducted for sample models using ground-glass plates and fluid flow, human finger, human wrist and an anesthetized rat to confirm the feasibility of the proposed parameters.

  16. A prototype of an integrated blood pressure and electrocardiogram device for multi-parameter physiologic monitoring

    Microsoft Academic Search

    S. Ahmad; S. Chen; K. Soueidan; I. Batkin; M. Bolic; H. Dajani; V. Groza

    2010-01-01

    We present a prototype of an integrated blood pressure (BP) and electrocardiogram (ECG) device for multi-parameter physiologic monitoring. A standard BP pressure cuff and an ordinary wristband have been modified to incorporate in them dry ECG electrodes made of thin conductive fabric. The modified BP cuff and wristband are coupled with commercially available hardware and software to harvest simultaneous arterial

  17. Effect of icodextrin on volume status, blood pressure and echocardiographic parameters: A randomized study

    Microsoft Academic Search

    Constantijn JAM Konings; Jeroen P Kooman; Marc Schonck; Ulrich Gladziwa; Joris Wirtz; A Warmold Van Den Wall Bake; Paul G Gerlag; Steven J Hoorntje; Johannes Wolters; Frank M Van Der Sande; Karel ML Leunissen

    2003-01-01

    Effect of icodextrin on volume status, blood pressure and echocardiographic parameters: A randomized study. Overhydration is a risk factor for hypertension and left ventricular hypertrophy in peritoneal dialysis patients. Recently, a high prevalence of subclinical overhydration was observed in peritoneal dialysis patients. Aim of the present open-label randomized study was to assess the effect of a icodextrin 7.5% solution on

  18. Effects of dietary onion (Allium cepa) powder on growth, innate immune response and hemato-biochemical parameters of beluga (Huso huso Linnaeus, 1754) juvenile.

    PubMed

    Akrami, Raza; Gharaei, Ahmad; Mansour, Majid Razeghi; Galeshi, Ali

    2015-08-01

    The present study was aimed at determining the effects of dietary onion powder on growth, innate immune response and hemato-biochemical parameters of beluga juvenile (Huso huso). Basal diets containing onion powder 0 (control), 0.5 and 1% of feed were fed to beluga juvenile. At the end of the experiment, the highest weight gain (WG%) and specific growth rate (SGR) was observed in group fed with 1% onion (P < 0.05). There were no significant difference (P > 0.05) about feed conversion ratio (FCR) in treatment groups that fed diets containing various levels of onion powder. After 8 weeks, serum lysozyme activity, superoxide dismutase activity (SOD), respiratory burst activity and serum total immunoglobulin (Ig) showed a significant increase in treatment group with 1% onion powder compared to other groups (P < 0.05). The group fed 1% onion showed a significantly increases in the number of erythrocytes (RBC), leucocyte (WBC), haematocrit (Hct) levels compared to the control group (P < 0.05). Haemoglobin, monocyte, lymphocyte and neutrophil had no significant change (P > 0.05) in treatment groups and control. The analysis of AST and LDH levels showed a significant decrease in 1% onion compared to the control and 0.5% onion diet (P < 0.05), while ALT and ALP levels were not influenced (P > 0.05). The blood glucose, total protein, triglyceride, cholesterol, albumin and globulin levels were lower in treated groups compared with the control (P < 0.05). The results of this study demonstrated that dietary onion powder could be an improvement in growth, hematological parameters and immune function of beluga juvenile. PMID:26067169

  19. Effect of Pesticide Exposure on Immunological, Hematological and Biochemical Parameters in Thai Orchid Farmers—A Cross-Sectional Study

    PubMed Central

    Aroonvilairat, Soraya; Kespichayawattana, Wannapa; Sornprachum, Thiwaree; Chaisuriya, Papada; Siwadune, Taweeratana; Ratanabanangkoon, Kavi

    2015-01-01

    Various studies have found that many Thai orchid farmers used excessive amounts of pesticides without proper protective gear, but no toxicological study has been made. This cross-sectional study aimed to evaluate the immunological, hematological and biochemical statuses of these farmers. Sixty four orchid farmers and 60 controls were studied. Plasma cholinesterase activity, the percentage and absolute number of B lymphocytes (CD19+) were significantly lower in the farmers group (3966.32 ± 1165.48 U/L, 11.61 ± 4.09% and 312.26 ± 164.83 cells/mm3, respectively) as compared to those of controls (5048.85 ± 1139.40 U/L, 14.32 ± 4.23%, 420.34 ± 195.18 cells/mm3, respectively). There was a statistically significant higher level of serum IgE among the orchid farmers (0.031 ± 0.011 mg/dL vs. 0.018 ± 0.007 mg/dL) but not IgG, IgA and IgM, levels. Serum lysozyme level, lymphocyte proliferative responses to mitogens, hematological parameters and kidney function test, were not significantly different between the two groups. The liver function profiles showed significantly lower levels of albumin and serum protein in the farmer group. Thus frequent pesticide exposure resulted in subtle changes of some biological parameters. These changes, though may not be clinically significant, strongly indicated that caution in handing pesticides by these farmers is warranted. PMID:26024358

  20. Unlimited access to low-energy diet causes acute malnutrition in dams and alters biometric and biochemical parameters in offspring.

    PubMed

    do Nascimento, E; de Santana Muniz, G; das Graças de Santana Muniz, M; de Souza Alexandre, L; da Rocha, L S; Leandro, C G; de Castro, R M; Bolaños-Jimenez, F

    2014-02-01

    Here we analyze the outcomes of unlimited access to a low-energy (LE) diet in dams and their offspring. At 3 weeks' gestation, pregnant Wistar rats were divided into two groups: (1) the control group received a normoenergetic diet; and (2) the experimental group received the LE diet. In dams, lactation outcomes, food intake, body weight, plasma IGF-1, prealbumin, transferrin and retinol-binding protein levels were evaluated; in offspring, biometric and biochemical parameters and food intake were evaluated. No differences were observed during pregnancy. However, after lactation, dams that received the LE diet demonstrated significant reductions in body weight (P<0.05), plasma IGF-1 (P=0.01), prealbumin and visceral fat (P<0.001). Pups born to dams that received the LE diet demonstrated reduced body length and weight at weaning (P<0.001) and were lighter than the control animals at the end of the experimental period. Pups also demonstrated reduced plasma, low-density lipoprotein (P=0.04), triglycerides (P=0.002) and glucose levels (P<0.05), and differences were noted in visceral fat. These results indicate that feeding dams with LE diet during the reproductive period induces acute malnutrition and impairs the growth and development of offspring, as well as certain metabolic parameters. PMID:24847690

  1. Effect of pesticide exposure on immunological, hematological and biochemical parameters in thai orchid farmers- a cross-sectional study.

    PubMed

    Aroonvilairat, Soraya; Kespichayawattana, Wannapa; Sornprachum, Thiwaree; Chaisuriya, Papada; Siwadune, Taweeratana; Ratanabanangkoon, Kavi

    2015-01-01

    Various studies have found that many Thai orchid farmers used excessive amounts of pesticides without proper protective gear, but no toxicological study has been made. This cross-sectional study aimed to evaluate the immunological, hematological and biochemical statuses of these farmers. Sixty four orchid farmers and 60 controls were studied. Plasma cholinesterase activity, the percentage and absolute number of B lymphocytes (CD19+) were significantly lower in the farmers group (3966.32 ± 1165.48 U/L, 11.61 ± 4.09% and 312.26 ± 164.83 cells/mm3, respectively) as compared to those of controls (5048.85 ± 1139.40 U/L, 14.32 ± 4.23%, 420.34 ± 195.18 cells/mm3, respectively). There was a statistically significant higher level of serum IgE among the orchid farmers (0.031 ± 0.011 mg/dL vs. 0.018 ± 0.007 mg/dL) but not IgG, IgA and IgM, levels. Serum lysozyme level, lymphocyte proliferative responses to mitogens, hematological parameters and kidney function test, were not significantly different between the two groups. The liver function profiles showed significantly lower levels of albumin and serum protein in the farmer group. Thus frequent pesticide exposure resulted in subtle changes of some biological parameters. These changes, though may not be clinically significant, strongly indicated that caution in handing pesticides by these farmers is warranted. PMID:26024358

  2. Effects of dopamine on retinal and choroidal blood flow parameters in humans

    PubMed Central

    Huemer, Karl?Heinz; Zawinka, Claudia; Garhöfer, Gerhard; Golestani, Elisabeth; Litschauer, Brigitte; Dorner, Guido T; Schmetterer, Leopold

    2007-01-01

    Aim To investigate the effect of dopamine on retinal and choroidal blood flow in humans. Methods We investigated the effect of two doses of intravenous dopamine (5 and 10?µg/kg/min) via a randomised double?masked crossover study in 12 healthy subjects chosen from a total of 16. Blood flow parameters in retina, optic nerve head and choroid were assessed with bi?directional laser Doppler velocimetry, laser Doppler flowmetry and laser interferometric measurement of fundus pulsation amplitude, respectively. Results Intravenous dopamine dose?dependently increased retinal blood cell velocity and fundus pulsation amplitude (p<0.001). At the highest administered dose red blood cell velocity in retinal vessels increased by 37% and fundus pulsation amplitude by 24%. By contrast, optic nerve head blood flow did not change with dopamine administration. Conclusions Our data indicate that dopamine has a pronounced enhancing effect on the retinal perfusion in humans. Further studies are required to establish the exact role of dopamine in the regulation of choroidal and optic nerve head blood flow. PMID:17383995

  3. EFFECTS OF ACUTE EXPOSURE TO CONCENTRATED AMBIENT PARTICULATES ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS HEALTHY AND MONOCROTALINE-TREATED SPRAGUE-DAWLEY RATS

    EPA Science Inventory

    EFFECTS OF ACUTE EXPOSURE TO CONCENTRATED AMBIENT PARTICULATES ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN HEALTHY AND MONOCROTALINE-TREATED SPRAGUE-DAWLEY RATS. LB Wichers1, JP Nolan2, DW Winsett2, UP Kodavanti2, MCJ Schladweiler2, DL Costa2, and WP ...

  4. Hemato-immunological and biochemical parameters of silver catfish Rhamdia quelen immunized with live theronts of Ichthyophthirius multifiliis.

    PubMed

    Tancredo, Karen R; Gonçalves, Eduardo L T; Brum, Aline; Acchile, Monyele; Hashimoto, Gabriela S O; Pereira, Scheila A; Martins, Maurício L

    2015-08-01

    This study investigated the immunization by intraperitoneal injection (i.p.) (assay I) and immersion bath (assay II) with live theronts of Ichthyophthirius multifillis in Rhamdia quelen and its influence on the hemato-immunological and biochemical parameters. Fish were divided in control (non immunized no challenged); non immunized and challenged with 12,000 theronts/fish; non immunized and challenged with 22,000 theronts/fish; immunized and challenged with 12,000 theronts/fish; immunized and challenged with 22,000 theronts/fish. Six days after challenge, either in the assay I or in the assay II the prevalence of I. multifillis in the gills was higher in non immunized fish (33.33% and 27.77%, respectively). In the assay I showed higher numbers of thrombocytes, leukocytes, lymphocytes, neutrophils and monocytes 20 days after injection and lower numbers after challenge. The immunoglobulin values were higher in fish non immunized. Fish immunized by immersion bath (assay II) showed greater values of catalase (CAT) in the liver (1245.49 U/mgprt) when compared to i.p. (198.79 U/mgprt). The levels of CAT in the liver of fish from the assay II were greater (1738.47 U/mgprt) 14 days after immunization than that observed 21 days after (1114.26 U/mgprt). The vaccination by i.p method showed influence on the hematological parameters. On the other hand, the immersion bath vaccination showed greater influence on the catalase activity in the liver. The results showed that new parameters like total protein, immunoglobulin and antioxidant enzymes could be considered in evaluating the host response to infection. PMID:26027759

  5. Effect of Aqueous Extract of Passiflora edulis on Biochemical and Hematological Parameters of Wistar Albino Rats

    PubMed Central

    Devaki, K.; Beulah, U.; Akila, G.; Gopalakrishnan, V. K.

    2012-01-01

    Passiflora edulis is traditionally used in folk lore medicine for the treatment of various ailments. To validate its use in traditional medicine, it is important to evaluate its toxicity in the animal system. Therefore, this study aimed to evaluate the toxicological effects of oral administration of aqueous leaf extract of P. edulis in Wistar albino rats. Acute toxicity tests were conducted by the oral administration of 200, 500, 1000 and 2000 mg/kg body weight of the animal. In subacute study, they were administered with various doses of aqueous extract of P. edulis (100, 200, 300, and 400 mg/kg body weight) to evaluate its toxicity for a period of 7 days. The effect of aqueous extract of P. edulis on organ weight, hematological, renal, and hepatic markers were analyzed. In acute toxicity study, no mortality was seen with in 24 h of the administration of P. edulis extract. No signs of neurological and behavioral changes were noticed with in 72 h. In the subacute study, the extract intake has not changed the hematological parameters such as RBC, WBC, and platelets and it was also found that the plasma level of amino transferases, ALP, urea, uric acid and, creatinine were also not altered by the administration of P. edulis extract throughout the study. The weight of organ was found to be unaltered in all the doses selected. The acute toxicity study reveals that the oral administration of the extract was found to be safe up to the dose level of 2000 mg/kg. The subacute study indicates that the extract is safe on the bone marrow function and it is neither hepatotoxic nor nephrotoxic. This supports the safety use of the aqueous extract of P. edulis in pharmacological studies. PMID:22736906

  6. Effect of Aqueous Extract of Passiflora edulis on Biochemical and Hematological Parameters of Wistar Albino Rats.

    PubMed

    Devaki, K; Beulah, U; Akila, G; Gopalakrishnan, V K

    2012-01-01

    Passiflora edulis is traditionally used in folk lore medicine for the treatment of various ailments. To validate its use in traditional medicine, it is important to evaluate its toxicity in the animal system. Therefore, this study aimed to evaluate the toxicological effects of oral administration of aqueous leaf extract of P. edulis in Wistar albino rats. Acute toxicity tests were conducted by the oral administration of 200, 500, 1000 and 2000 mg/kg body weight of the animal. In subacute study, they were administered with various doses of aqueous extract of P. edulis (100, 200, 300, and 400 mg/kg body weight) to evaluate its toxicity for a period of 7 days. The effect of aqueous extract of P. edulis on organ weight, hematological, renal, and hepatic markers were analyzed. In acute toxicity study, no mortality was seen with in 24 h of the administration of P. edulis extract. No signs of neurological and behavioral changes were noticed with in 72 h. In the subacute study, the extract intake has not changed the hematological parameters such as RBC, WBC, and platelets and it was also found that the plasma level of amino transferases, ALP, urea, uric acid and, creatinine were also not altered by the administration of P. edulis extract throughout the study. The weight of organ was found to be unaltered in all the doses selected. The acute toxicity study reveals that the oral administration of the extract was found to be safe up to the dose level of 2000 mg/kg. The subacute study indicates that the extract is safe on the bone marrow function and it is neither hepatotoxic nor nephrotoxic. This supports the safety use of the aqueous extract of P. edulis in pharmacological studies. PMID:22736906

  7. Effect of Replacing Wheat Straw with Almond Hull and Shell in Diets on Nutrient Digestibility and Blood Parameters of Goat

    Microsoft Academic Search

    A. Can; N. Denek; M. ?eker

    2007-01-01

    Can, A., Denek, N. and ?eker, M. 2007. Effect of replacing wheat straw with almond hull and shell in diets on nutrient digestibility and blood parameters of goat. J. Appl. Anim. Res., 32: 181–183.To determine the effect of replacing wheat straw with almond hulls and shell in diets on nutrient digestibility and blood parameters of goat, eight male Kilis goats

  8. Blood pressure parameters and carotid intraplaque hemorrhage as measured by magnetic resonance imaging: The Rotterdam Study.

    PubMed

    Selwaness, Mariana; van den Bouwhuijsen, Quirijn J A; Verwoert, Germaine C; Dehghan, Abbas; Mattace-Raso, Francesco U S; Vernooij, Meike; Franco, Oscar H; Hofman, Albert; van der Lugt, Aad; Wentzel, Jolanda J; Witteman, Jacqueline C M

    2013-01-01

    Intraplaque hemorrhage (IPH) is a characteristic of the vulnerable atherosclerotic plaque that has been associated with ischemic stroke. Not much is known about determinants of IPH. We studied whether blood pressure parameters are associated with presence of IPH. Within the framework of a prospective population-based cohort study, The Rotterdam Study, the carotid arteries of 1006 healthy participants ?45 years and with intima-media thickening (?2.5 mm) on ultrasound were imaged with a 1.5-T magnetic resonance imaging scanner. IPH was defined as a hyperintense signal on a 3D-T1w-GRE magnetic resonance sequence. Generalized estimation equation analysis, adjusted for age, sex, carotid wall thickness, and cardiovascular risk factors, was used to assess the association between blood pressure parameters and IPH. Magnetic resonance imaging of the carotid arteries revealed presence of IPH in 444 of 1860 plaques (24%). Systolic blood pressure and pulse pressure (PP) were significantly associated with IPH after adjustment for age and sex. In multivariate analysis, PP yielded the strongest association, with an odds ratio per SD increase in PP of 1.22 (95% CI, 1.07-1.40). The odds ratio per SD for systolic blood pressure was 1.13 (0.99-1.28). Only PP remained significant after additional adjustment for other blood pressure components. The combination of smoking and isolated systolic hypertension was associated with 2.5 times increased risk of IPH (1.2-5.2). In conclusion, PP was the strongest determinant of IPH independent of cardiovascular risk factors and other blood pressure components. The association between pulsatile flow and IPH may provide novel insights in the development of the vulnerable plaque. PMID:23213192

  9. Protective effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna subjected to hydrogen peroxide.

    PubMed

    Bownik, Adam; St?pniewska, Zofia

    2015-04-01

    Ectoine (ECT) is an osmoprotectant produced by halophilic microorganisms inducing protective effects against various stressful factors. However, little is known about its influence on aquatic invertebrates subjected to hydrogen peroxide (H2O2)-a commonly used oxidative disinfectant. Therefore, the aim of our study was to determine the effects of H2O2 alone (at 5 and 10 mg/L) and in the combination with various concentrations of ECT (5, 10 and 25 mg/L) on behavioral, physiological and biochemical parameters of Daphnia magna. The following endpoints were determined: mortality, heart rate, thoracic limb movement, total glutathione (GSH)/oxidized glutathione (GSSG) ratio, catalase (CAT) activity and nitric oxide (NOx) level. The study showed that daphnids exposed to the combination of H2O2+ECT showed decreased mortality, attenuated inhibition of heart rate and thoracic limb activity, less decreased GSH/GSSG ratio, lower stimulation of CAT activity and NOx level when compared to the crustaceans exposed to H2O2 alone. The most pronounced alleviation of toxic effects was observed in the combination of 5 mg/L H2O2+25 mg/L ECT. The results suggest that protective effects of ECT in D. magna subjected to H2O2 may be related to antioxidative properties of the osmoprotectant. PMID:25704915

  10. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation.

    PubMed

    Mishra, Arun K; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-09-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and ?-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue. PMID:23008814

  11. The effects of dietary boron compounds in supplemented diet on hormonal activity and some biochemical parameters in rats.

    PubMed

    Kucukkurt, Ismail; Akbel, Erten; Karabag, Funda; Ince, Sinan

    2015-03-01

    The aims of this study were to clarify the effects of dietary boric acid or borax, as a boron (B) source, on hormonal status (leptin, insulin, triiodothyronine (T3), and thyroxine) and some biochemical parameter levels as glucose, carnitine, nonesterified fatty acids, and betahydroxybutyric acid in rats. A total of 30 Sprague-Dawley male rats were divided into three equal groups: the animals in the first group (control) were fed with a standard rodent diet containing 6.4 mg B/kg, and the animals in the experimental group were fed with a standard rodent diet added with boric acid and borax (100 mg B/kg) throughout the experimental period of 28 days. The B compounds especially borax decreased leptin, insulin, and glucose levels, whereas increased T3 and carnitine levels in plasma. In addition, body weight of rats was found to be low in the boric acid group at the end of 4 weeks. Consequently, our results demonstrate that B supplementation (100 mg/kg) in diet decreases body weight, leptin, and insulin, whereas increases T3 levels in plasma, so enhances the metabolic activity of rats. Between the B compounds used in this study, it was found that borax had a greater effect on hormonal status than boric acid. PMID:23293135

  12. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation

    PubMed Central

    Mishra, Arun K.; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-01-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and ?-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue. PMID:23008814

  13. Toxicity of anthraquinones: differential effects of rumex seed extracts on rat organ weights and biochemical and haematological parameters.

    PubMed

    Islam, Rabigul; Mamat, Yultuz; Ismayil, Ilyar; Yan, Ming; Kadir, Mahsutjan; Abdugheny, Abdujilil; Rapkat, Haximjan; Niyaz, Mardan; Ali, Yusupjan; Abay, Sirapil

    2015-05-01

    The genus Rumex and related species such as Rheum and Polygonum are widely used as medicinal herbs and foods. They contain anthraquinones (AQ) such as emodin and chrysophanol as active ingredients, and there is concern about the toxicity of these compounds. This study evaluated the chronic effects of Rumex patientia seed aqueous and ethanolic extracts, in male and female rats separately, on organ weights and over 30 haematological, biochemical and histological parameters, immediately after 14-week administration and after a further period of 15?days without drug treatment. Adverse changes were associated with long-term AQ administration, and these focussed on the liver, lung and kidney, but after 15-day convalescence, most had reverted to normal. In general, male rats appeared to be more susceptible than female rats at similar doses. The water extract produced no irreversible changes, which may reflect the lower dose of the AQ constituents or the presence of different ancillary compounds, and supports the traditional method of extracting Rumex seeds with water. In conclusion, ethanolic extracts of R. patientia caused irreversible pathological changes at very high doses (4000mg/kg), but lower doses and aqueous extracts produced either non-significant or reversible changes. Long-term administration of high doses of AQ extracts over a long period of time should be avoided until further assurances can be given, and given other existing reports of reproductive toxicity, should be avoided altogether during pregnancy. Copyright © 2015 John Wiley & Sons, Ltd. PMID:25753342

  14. Lumped-parameter tissue temperature-blood perfusion model of a cold-stressed fingertip.

    PubMed

    Shitzer, A; Stroschein, L A; Gonzalez, R R; Pandolf, K B

    1996-05-01

    A lumped-parameter model of a fingertip is presented. The semispherical model includes the effects of heat storage, heat exchange with the environment, and heat transport by blood perfusion. The thermal insulation on the surface of the fingertip is represented by the overall heat transfer coefficient that is calculated by common engineering formulas. The model is solved analytically for the simple case of constant blood perfusion rate. The general case of variable blood perfusion rates is solved by an Euler finite difference technique. At this stage, the model does not include active control mechanisms of blood perfusion. Thus the effects of cold-induced vasodilatation have to be superimposed and are modeled by symmetrical triangular waveforms because these were found to best depict the behavior of fingers exposed to cold environments. Results of this model were compared with experimental data obtained in two separate studies. One included 60-min infrared thermograms of the dorsal surface of bare hands of sedentary subjects horizontally suspended on a fish net in a 0 degree C environment. Another study, on gloved finger temperatures, involved 0 and -6.7 degrees C environments. Fingertip (nail bed) temperatures of both these studies were compared with model predictions. Blood perfusion rates were assumed and adjusted within physiologically reasonable limits. Comparison of measured and computed temperature records showed very good conformity in both cases studied. PMID:8727573

  15. Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.

    PubMed

    Rao, Meng; Zhao, Xiao-Ling; Yang, Jing; Hu, Shi-Fu; Lei, Hui; Xia, Wei; Zhu, Chang-Hong

    2015-01-01

    In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively). The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031). We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress. PMID:25652627

  16. Pregnancy Dating in the Rat: Placental Morphology and Maternal Blood Parameters

    Microsoft Academic Search

    Eveline P. C. T. de Rijk; Eric van Esch; Gert Flik

    2002-01-01

    The rat is commonly used as a model in studies on embryology and reproduction toxicology. Surprisingly, the current literature does not provide a comprehensive reference data set on placental development in rat. Therefore, we have evaluated morphological changes of the placenta and maternal blood parameters duringpregnancyof theSprague-Dawleyrat. Themorphologicdatapresentedinthis studymaybe usefulasreferencematerial. This studyrevealedthatplacentaldevelopmentintheratisawell-deé nedprocess,characterizedby keysynchronizedmorphologicaleventsatspeciécpoints in time, convenient for laboratory practice

  17. Morin attenuates blood pressure and oxidative stress in deoxycorticosterone acetate-salt hypertensive rats: a biochemical and histopathological evaluation.

    PubMed

    Prahalathan, Pichavaram; Kumar, Subramanian; Raja, Boobalan

    2012-08-01

    The present study was designed to evaluate the antihypertensive and antioxidant effect of morin, a flavonoid against deoxycorticosterone acetate (DOCA)-salt induced hypertension in male Wistar rats. Hypertension was induced in uninephrectomized rats (UNX) by weekly twice subcutaneous injection of DOCA (25mg/kg) and 1% NaCl in the drinking water for six consecutive weeks. The DOCA-salt hypertensive rats showed significant (P < .05) increase in the systolic and diastolic blood pressure, heart rate, water intake and organ weights (kidney, heart, aorta and liver). DOCA-salt hypertensive rats also showed significant (P < .05) increase in the levels of thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes in plasma and tissues (kidney, heart, aorta and liver), and significant (P < .05) decrease in the body weight, nitrite and nitrate levels in plasma and heart. Furthermore, the activities of enzymic antioxidants such as superoxide dismutase, catalase and glutathione peroxidase in erythrocyte and tissues and the levels of non-enzymic antioxidants such as reduced glutathione, vitamin C and vitamin E in plasma and tissues were significantly (P < .05) decreased in DOCA-salt rats. Morin supplementation (50mg/kg) daily for six weeks brought back all the above parameters to near normal level. The above findings were confirmed by the histopathological examination. No significant (P < .05) effect was observed in UNX-rats treated with morin (50mg/kg). These results suggest that morin acts as an antihypertensive and antioxidant agent against DOCA-salt induced hypertension. PMID:22386933

  18. Multivariate Discriminant Function Based on Six Biochemical Markers in Blood Can Predict the Cirrhotic Evolution of Chronic Hepatitis

    Microsoft Academic Search

    Giuliana Fortunato; Giuseppe Castaldo; Giovannangelo Oriani; Raimondo Cerini; Mariano Intrieri; Eugenia Molinaro; Ivan Gentile; Guglielmo Borgia; Marcello Piazza; Francesco Salvatore; Lucia Sacchetti

    Background: Serologic markers have been proposed for monitoring hepatic fibrosis in chronic active liver dis- ease. Because none of these markers, when used singly, is totally satisfactory, we developed and evaluated a multivariate approach. Methods: We studied two cohorts of chronic hepatitis (54 patients) and cirrhosis patients (49 patients) to iden- tify a panel of biochemical markers that discriminates between

  19. Blood-stimulatory and biochemical effects of intravenous laser irradiation of blood with low-active laser radiation in rabbits with peripheral nerve damage

    Microsoft Academic Search

    Leonid V. Tanin; G. G. Petrovsky; Raisa M. Tanina

    1996-01-01

    It is found that in rabbits with the injured sciatic nerve irradiation of blood with low-intensity laser radiation stimulates the mechanism of hemoglobin and thrombocyte formation. Normalization of the lymphocytic composition in blood of the animals with the injured nerve shows that it is possible to prevent development of the process of lymphocytopenia. Changes in the albumins\\/globulins ratio, the stimulating

  20. Effects of Dietary Zinc and l Arginine Supplementation on Total Antioxidants Capacity, Lipid Peroxidation, Nitric Oxide, Egg Weight, and Blood Biochemical Values in Japanase Quails

    Microsoft Academic Search

    Onur Atakisi; Emine Atakisi; Asim Kart

    2009-01-01

    The aim of this study was to evaluate effects of dietary zinc and l-arginine supplementation on blood total antioxidant capacity (TAC), malondialdehyde (MDA), nitric oxide (NO), some blood\\u000a chemistry parameters, and egg weights of laying quails. Three groups of Japanese quails were fed with a diet containing l-arginine (5 mg\\/kg), zinc (60 mg\\/kg), and normal basal diet (control) for 30 days. TAC, lipid

  1. Noninvasive measurement of postocclusive parameters in human forearm blood by near infrared spectroscopy

    NASA Astrophysics Data System (ADS)

    Rao, K. Prahlad; Radhakrishnan, S.; Reddy, M. Ramasubba

    2005-04-01

    Near infrared (NIR) light in the wavelength range from 700 to 900 nm can pass through skin, bone and other tissues relatively easily. As a result, NIR techniques allow a noninvasive assessment of hemoglobin saturation for a wide range of applications, such as in the study of muscle metabolism, the diagnosis of vascular disorders, brain imaging, and breast cancer detection. Near infrared Spectroscopy (NIRS) is an effective tool to measure the hemoglobin concentration in the tissues, which can discriminate optically the oxy- and deoxy- hemoglobin species because of their different near-infrared absorption spectra. We have developed an NIRS probe consisting of a laser diode of 830 nm wavelength and a PIN photodiode in reflectance mode. We have selected a set of healthy volunteers (mean age 30, range 26-40 years) for the study. The probe is placed on forearm of each subject and the backscattered light intensity is measured by occluding the blood flow at 210, 110 and 85 mmHg pressures. Recovery time, peak time and time after 50% release of the cuff pressure are determined from the optical densities during the post occlusive state of forearm. These parameters are useful for determining the transient increase in blood flow after the release of blood occlusion. Clinically, the functional aspects of blood flow in the limbs could be evaluated noninvasively by NIRS.

  2. Effect of dental base metal alloys on IgE levels and some blood parameters.

    PubMed

    Kulak, Y; Arikan, A

    1997-10-01

    Despite the widespread use of nickel-based alloys, claims for safety of these alloys have not yet been accepted universally. The allergenic effects of nickel on dental patients and the potential toxic effects of nickel and beryllium on laboratory technicians continue to cause concern within the dental profession. The purpose of this study was to investigate immunoglobulin type E (IgE) values and some blood parameters of dental laboratory technicians who use dental base metal alloys. The following two groups were studied: 19 students who had been working with dental base metal alloys for two years; and 21 pre-clinical students who had never worked with dental base metal alloys. The latter group were used as a control. Blood specimens were taken from both groups and analysed using The Blood Counter. Total erythrocyte, thrombocyte, leukocyte, lymphocyte, granulocyte and monocyte counts were determined. Measurement of IgE was made with Coat-A-Count Total IgE IRMA. Blood and IgE measurements were repeated after 8 months. In the experimental group both erythrocyte and thrombocyte values were found to be statistically significantly decreased compared with the control group. No significant differences were found in lymphocyte and monocyte numbers between the initial and later measurements. There were no significance changes in IgE values for both groups. These results provide no evidence that dental base metal alloys (Ni, Cr, Be, Co) caused an increase in sensitization, during the period of the study. PMID:9372465

  3. Quantization and diagnosis of Shanghuo (Heatiness) in Chinese medicine using a diagnostic scoring scheme and salivary biochemical parameters

    PubMed Central

    2014-01-01

    Background This study aims to establish a diagnostic scoring scheme for Shanghuo (Heatiness) and to evaluate whether Shanghuo is associated with biochemical parameters of salivary lysozyme (LYZ), salivary secreted immunoglobulin (S-IgA), salivary amylase (AMS), and saliva flow rate (SFR). Methods We collected 121 Shanghuo patients at the Affiliated Hospitals of Guangzhou University of Traditional Chinese Medicine in Guangdong Province, 60 cases as a Shanghuo recovered group, and 60 healthy cases as a healthy control group. The diagnostic scoring scheme was established by probability theory and maximum likelihood discriminatory analysis on the basis of epidemiology with the design of self-controlled clinical trial. Subsequently, we used the same methods to collect 120 Shanghuo patients, 60 Shanghuo recovered cases, and 60 healthy cases in both Hunan Province and Henan Province. The levels of LYZ, S-IgA, AMS, and SFR were tested when the patients suffered from Shanghuo or recovered, respectively. Results The diagnostic score table for Shanghuo syndrome was established first. In the retrospective tests, the sensitivity, specificity, accuracy, and positive likelihood ratio of the diagnostic score table were 98.9%, 93.5%, 97.5%, and 14.34%, respectively. In the prospective tests, the corresponding values were 94.9%, 85.7%, 91.7%, and 6.64%, respectively. Shanghuo was classified into three degrees based on the diagnostic scores, common Shanghuo: 63–120; serious Shanghuo: 121–150; very serious Shanghuo: >150. A negative correlation was found between Shanghuo and S-IgA (R?=?-0.428; P?=?0.000). The level of S-IgA was also affected by seasonal and regional factors. No significant correlations were found between Shanghuo and the levels of LYZ, AMS, and SFR. Conclusions In this study, Shanghuo could be diagnosed by the combination of the diagnostic score table and S-lgA level. PMID:24386887

  4. Effects of dietary extra-virgin olive oil on behaviour and brain biochemical parameters in ageing rats.

    PubMed

    Pitozzi, Vanessa; Jacomelli, Michela; Zaid, Mohamed; Luceri, Cristina; Bigagli, Elisabetta; Lodovici, Maura; Ghelardini, Carla; Vivoli, Elisa; Norcini, Monica; Gianfriddo, Marco; Esposto, Sonia; Servili, Maurizio; Morozzi, Guido; Baldi, Elisabetta; Bucherelli, Corrado; Dolara, Piero; Giovannelli, Lisa

    2010-06-01

    The aim of the present study was to verify whether extra-virgin olive oil, a dietary component naturally containing phenolic antioxidants, has the potential to protect the brain from the deleterious effects of ageing. To accomplish this goal, we used male rats fed a high-energy diet containing either maize oil, or extra-virgin olive oil with high or low phenol content (720 or 10 mg total phenols/kg oil, corresponding to a daily dose of 4 or 0.05 mg total phenols/kg body weight, respectively) from age 12 months to senescence. The measured endpoints were biochemical parameters related to oxidative stress and functional tests to evaluate motor, cognitive and emotional behaviour. Olive oil phenols did not exert major protective actions on motor and cognitive function, as we observed only a tendency to improved motor coordination on the rotarod in the old animals treated with the oil rich in phenols (40 % average increase in the time to first fall; P = 0.18). However, an interesting finding of the present study was a reduced step-through latency in the light-dark box test, found in the older animals upon treatment with the oil rich in antioxidant phenols, possibly indicating an anxiety-lowering effect. This effect was associated with decreased glutathione reductase activity and expression in the brain, a phenomenon previously associated with decreased anxiety in rodents. These results indicate a previously undetected effect of a diet containing an olive oil rich in phenols. Further studies are warranted to verify whether specific food antioxidants might also have an effect on emotional behaviour. PMID:20070918

  5. Vibration white finger, digital blood pressure, and some biochemical findings on workers operating vibrating tools in the engine manufacturing industry.

    PubMed

    Bovenzi, M

    1988-01-01

    A clinical and laboratory investigation was carried out on 76 operators using pneumatic hand grinders and impact wrenches in the engine manufacturing industry. Twenty-two vibration-exposed workers (28.9%) had no symptoms in the hands (stage 0 of the Stockholm Workshop scale), 34 (44.7%) were affected with sensorineural disturbances in the fingers (stage SN), and 20 (26.3%) suffered from vibration white finger (VWF stages 1-2-3). In the vibration-exposed operators and in 30 comparable referents not exposed to vibration, finger systolic blood pressure (FSP) was measured on a test finger and on a control finger after digit cooling to 30 degrees C and 10 degrees C. The results of the cold provocation test were expressed as percent change of FSP by cooling the test finger from 30 degrees C to 10 degrees C (FSP%t, 10 degrees) and as digital/brachial pressure index during local cooling at 10 degrees C (DPIt, 10 degrees). After cold provocation the mean values of FSP%t, 10 degrees and DPIt 10 degrees were more significantly reduced in the vibration-exposed workers with VWF than in those without VWF and the referents (p less than 0.001). The cold provocation test was found to differentiate between VWF subjects with stages 1-2 and stage 3 (p less than 0.02). It is concluded that the measurement of FSP combined with finger cooling is a useful laboratory test to diagnose objectively Raynaud's phenomenon of occupational origin. The vibration-exposed workers and the referents were also tested for serum levels of immunoglobulins and complement and for daily excretion of urinary free catecholamines. Between the reference and vibration groups no differences in the mean values of the immunologic parameters and urinary catecholamines were found. The meaning of these findings is discussed. PMID:3228071

  6. Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization.

    PubMed

    Kosti?, Ivana T; Ili?, Vesna Lj; ?or?evi?, Verica B; Bukara, Katarina M; Mojsilovi?, Slavko B; Nedovi?, Viktor A; Bugarski, Diana S; Veljovi?, ?or?e N; Miši?, Danijela M; Bugarski, Branko M

    2014-10-01

    The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35mM hypotonic sodium-phosphate/NaCl, enabling >90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts. PMID:25051307

  7. Effect of zinc supplementation from inorganic and organic sources on growth and blood biochemical profi le in crossbred calves

    Microsoft Academic Search

    G. P. Mandal; R. S. Dass; A. K. Garg; V. P. Varshney; A. B. Mondal

    The effect of zinc supplementation from inorganic and organic sources on some physiological and biochemical profi le was investigated in 15 male crossbred calves (age 14-15 months, liveweight 226.0 ± 9.06 kg) randomly divided into three groups of fi ve animals in each. Animals in group I (control) were fed a basal diet comprised of wheat straw and concentrate mixture

  8. Study of the protective effect of ascorbic acid against the toxicity of stannous chloride on oxidative damage, antioxidant enzymes and biochemical parameters in rabbits

    Microsoft Academic Search

    M. I. Yousef; T. I. Awad; F. A. Elhag; F. A. Khaled

    2007-01-01

    Stannous chloride (SnCl2) is a reducing chemical agent used in several man-made products. SnCl2 can generate reactive oxygen species (ROS). Therefore, the present study has been carried out to investigate the antioxidant action of l-ascorbic acid (AA) in minimizing SnCl2 toxicity on lipid peroxidation, antioxidant enzyme, and biochemical parameters in male New Zealand white rabbits. Animals were assigned to one

  9. Evaluation of Serum Magnesium, Lipid Profile and Various Biochemical Parameters as Risk Factors of Cardiovascular Diseases in Patients with Rheumatoid Arthritis

    PubMed Central

    Ramavataram, DVSS; Patel, Payal A.; Rupani, Mihir P.

    2015-01-01

    Background Rheumatoid arthritis (RA) is chronic inflammatory disease, associated with increased risk of cardiovascular diseases (CVD) than the general population. Chronic inflammatory conditions are likely to alter magnesium level and various biochemical parameters. Objectives To study the probable changes in serum magnesium, lipid profile and various biochemical parameters and to assess risk factors of CVD in newly diagnosed RA patients compared to controls. Materials and Methods We studied 50 newly diagnosed RA adult patients and 50 healthy individuals as controls. Serum magnesium, calcium, lipid profile, uric acid and other biochemical parameters were measured in study subjects. Results were expressed as Mean ± SD and compared between RA subjects and controls by Independent sample t-test and Pearson correlation. Results We found decreased serum magnesium and calcium in RA subjects compared to the controls (p < 0.001). RA subjects had atherogenic lipid profile characterized by elevated total cholesterol (p = 0.054), LDL cholesterol (p = 0.008) and decreased HDL cholesterol (p <0.001). Serum uric acid was higher in RA cases compared to controls (p = 0.025). Serum magnesium was negatively correlated with total cholesterol, LDL cholesterol and positively correlated with HDL cholesterol in RA cases. Conclusion Decreased magnesium level, dyslipidemia and increased uric acid observed in our study together may be more potent risk factors for CVD in newly diagnosed RA subjects. We recommend that serum magnesium should be investigated as a part of cardiovascular risk management in RA. We suggest that decreased serum magnesium and increased serum uric acid may be considered as nontraditional risk factors of CVD in RA. Further prospective studies are needed to confirm the impact of inflammation on various biochemical parameters and cardiovascular outcomes in patients with RA. PMID:26023546

  10. Dosimetric parameters as predictive factors for biochemical control in patients with higher risk prostate cancer treated with Pd-103 and supplemental beam radiation

    SciTech Connect

    Orio, Peter [Department of Radiation Oncology, University of Washington, Seattle, WA (United States); Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States); Wallner, Kent [Department of Radiation Oncology, University of Washington, Seattle, WA (United States) and Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States) and Group Health Cooperative, Seattle, WA (United States)]. E-mail: kent.Wallner@med.va.gov; Merrick, Gregory [Schiffler Cancer Center, Wheeling, WV (United States); Herstein, Andrew [Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States); Mitsuyama, Paul [Department of Radiation Oncology, University of Washington, Seattle, WA (United States); Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States); Thornton, Ken [Varian Medical Systems, Charlottesville, VA (United States); Butler, Wayne [Schiffler Cancer Center, Wheeling, WV (United States); Sutlief, Steven [Department of Radiation Oncology, University of Washington, Seattle, WA (United States); Radiation Oncology, Puget Sound Health Care System, Department of Veterans Affairs, Seattle, WA (United States)

    2007-02-01

    Purpose: To analyze the role of dosimetric quality parameters in maximizing cancer eradication in higher risk prostate cancer patients treated with palladium (Pd)-103 and supplemental beam radiation. Methods: One-hundred-seventy-nine patients treated with Pd-103 and supplemental beam radiation, with minimum 2 years follow-up prostate-specific antigen (PSA) values and posttreatment computed tomography scans were analyzed. Dosimetric parameters included the V100 (percent of the postimplant volume covered by the prescription dose), the D90 (the minimum dose that covered 90% of the post implant volume), and the treatment margins (the radial distance between the prostatic edge and the prescription isodose). Treatment margins (TMs) were calculated using premarket software. Results: Freedom from biochemical failure was 79% at 3 years, with 92 of the 179 patients (51%) followed beyond 3 years. In comparing patients who did or did not achieve biochemical control, the most striking differences were in biologic factors of pretreatment PSA and Gleason score. The V100, D90, and average TM all showed nonsignificant trends to higher values in patients with biochemical control. In multivariate analysis of each of the three dosimetric parameters against PSA and Gleason score, TM showed the strongest correlation with biochemical control (p = 0.19). Conclusions: For patients with intermediate and high-risk prostate cancer treated with Pd-103 brachytherapy and external beam radiation, biologic factors (PSA and Gleason score) were the most important determinants of cancer eradication. However, there is a trend to better outcomes among patients with higher quality implant parameters, suggesting that attention to implant quality will maximize the likelihood of cure.

  11. Correlation between Blood Activin Levels and Clinical Parameters of Type 2 Diabetes

    PubMed Central

    Wu, Hui; Wu, Michael; Chen, Yi; Allan, Carolyn A.; Phillips, David J.; Hedger, Mark P.

    2012-01-01

    Aims. Activins A and B, and their binding protein, follistatin, regulate glucose metabolism and inflammation. Consequently, their role in type 2 diabetes (T2D) was examined. Methods. Blood was taken from fasted participants (34 males; 58 females; 50–75 years) with diabetes or during an oral glucose tolerance test (OGTT). Clinical parameters were assessed, and blood assayed for activins, follistatin, and C-reactive protein. Results. Serum levels of activin A (93.3 ± 27.0?pg/mL, mean ± SD), B (81.8 ± 30.8?pg/mL), or follistatin (6.52 ± 3.15?ng/mL) were not different (P > 0.05) between subjects with normal OGTT (n = 39), impaired glucose tolerance and/or fasting glucose (n = 17), or T2D (n = 36). However, activin A and/or activin B were positively correlated with parameters of insulin resistance and T2D, including fasting glucose (P < 0.001), fasting insulin (P = 0.02), glycated hemoglobin (P = 0.003), and homeostasis model assessment of insulin resistance (HOMA-IR; P < 0.001). Follistatin was positively correlated with HOMA-IR alone (P = 0.01). Conclusions. These data indicate that serum measurements of activin A, B, or follistatin cannot discriminate risk for T2D in individual patients, but the activins display a positive relationship with clinical parameters of the disease. PMID:23304117

  12. Protective effect of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    PubMed

    Aghaei, S; Nikzad, H; Taghizadeh, M; Tameh, A A; Taherian, A; Moravveji, A

    2014-10-01

    Cancer treatment with cyclophosphamide (CP) may result in reproductive toxicity as one of its side effects. The pumpkin seed is a rich natural source of antioxidant. We have assessed the possible protective efficacy of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology of CP-treated rats. Male adult Wistar rats were categorised into four groups. Group 1 served as control and received intraperitoneal (IP) injection of isotonic saline solution. Group 2 rats were treated with CP by IP injection in a single dose of 100 mg/kg body weight, only once. Group 3 and 4 received CP plus 300 and 600 mg/kg pumpkin seed extract respectively. Six weeks after treatment, sperm characteristics, biochemical parameters and histopathological changes were examined. Results showed that, sperm characteristics in CP-treated rats were significantly decreased. Biochemical analysis results showed that the co-administration of 300 mg pumpkin seed extract could increase the total antioxidant capacity (TAC) level significantly. In CP-treated rats, histopathological changes such as vacuolisation, disorganisation and separation of epididymal epithelium were observed as well. Interestingly, pumpkin seed extract could improve the above-mentioned parameters remarkably in CP-treated rats. Our findings indicated that pumpkin seed extract might be used as protective agent against CP-induced reproductive toxicity. PMID:24124763

  13. Evaluation of Performance Traits in Brahman Cattle: Blood Parameters, Calf Temperament, Residual Feed Intake, and Bull Reproductive Development 

    E-print Network

    Matheney, Kara J.

    2010-10-12

    The objectives of these studies were (1) evaluate the relationship between temperament, blood parameters, and performance in Brahman calves (n = 300); (2) evaluate the relationship between residual feed intake (RFI) and reproductive development...

  14. Evaluation of Performance Traits in Brahman Cattle: Blood Parameters, Calf Temperament, Residual Feed Intake, and Bull Reproductive Development

    E-print Network

    Matheney, Kara J.

    2010-10-12

    The objectives of these studies were (1) evaluate the relationship between temperament, blood parameters, and performance in Brahman calves (n = 300); (2) evaluate the relationship between residual feed intake (RFI) and reproductive development...

  15. Effects of Dietary Protein and Lipids on Blood Parameters and Superoxide Anion Production in the Grouper, Epinephelus coioides (Serranidae: Epinephelinae)

    Microsoft Academic Search

    Ann-Chang Cheng; Chia-Yung Chen; Chyng-Hwa Liou; Ching-Fong Chang

    2006-01-01

    Ann-Chang Cheng, Chia-Yung Chen, Chyng-Hwa Liou, and Ching-Fong Chang (2006) Effects of dietary protein and lipids on blood parameters and superoxide anion production in the grouper, Epinephelus coioides (Serranidae: Epinephelinae). Zoological Studies 45(4): 492-502. The objectives of this study were to investi- gate the effects of dietary lipid and protein levels on blood parameters, body composition, body indices, and the

  16. Impact of maternal and neonatal factors on parameters of hematopoietic potential in umbilical cord blood

    PubMed Central

    Al-Deghaither, Sara Y.

    2015-01-01

    Objectives: To determine characteristics of laboratory parameters of hematopoietic potential in umbilical cord blood and their association with maternal and neonatal factors. Methods: This prospective analysis was performed on 206 umbilical cord blood donations (50-200 ml) from King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia between January and September 2014. Samples were processed and analyzed for total nucleated cells (TNC’s), cluster designation (CD)45+, CD34+ counts, nucleated red blood cells (NRBCs) count, and viability testing. Results: Most of the study participants (63.6%) were on their first 3 deliveries and from women with age between 17 and 30 years (80.6%). The donated volume was 50.4-192.4 ml, TNCs ranged from 500.2×106 to 9430.3 ×106 cells, and CD34+ cells ranged from 1.25×106 to 12.82×106/unit. The volume was positively affected by bigger birth weight of the baby (p<0.0001), larger placenta (p=0.001), TNCs (p<0.0001), CD34+ (p<0.0001), NRBCs (p<0.0001), and viability (p=0.002). There were no statistically significant differences between baby boys and girls for laboratory variables. Conclusion: In the selection and identification of a possible donor of umbilical cord blood, several maternal and neonatal factors should be considered, as younger maternal age, neonatal birth weight >3300 grams, larger placental size, and first or second-born babies, were shown to be associated with higher TNCs, CD34+, CD45+, NRBCs, and viability. PMID:25987113

  17. Relating fibropapilloma tumor severity to blood parameters in green turtles Chelonia mydas.

    PubMed

    Hirama, Shigetomo; Ehrhart, Llewellyn M; Rea, Lorrie D; Kiltie, Richard A

    2014-08-21

    Fibropapillomatosis is a neoplastic disease that is commonly found in the green turtles Chelonia mydas in tropical and subtropical regions of the world. In the current project, juvenile green turtles were captured with large-mesh tangle nets in the Indian River Lagoon and on nearshore reefs of Indian River County, Florida, USA, in 1998 and 1999. The purpose of the study was to determine the relationship between the severity of the disease and the general health of green turtles as indicated by blood parameters. All turtles were measured and examined, and the overall severity of the disease was rated by the size, number, and location of external fibropapilloma tumors. Hematocrit, total protein, and hemoglobin concentration were measured and compared with tumor scores (tumor severity appraisal). As the tumor score increased, the blood parameters of turtles decreased; for instance, the percentage of decrease in hematocrit for mildly afflicted, moderately afflicted, and severely afflicted groups were 2.6, 18.3, and 45.5%, respectively. Severely afflicted turtles suffered from anemia, while individuals with mild affliction did not. PMID:25144118

  18. Use of mid-infrared and near-infrared diffuse reflectance spectroscopy for characterization of soil biochemical parameters

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The ability to rapidly characterize the quality of soils within agricultural landscapes is limited by the ability to rapidly measure soil biochemical properties. Diffuse reflectance spectroscopic analysis in the near-infrared (NIR, 400-2500 nm) and mid-infrared (MIR 2500-25000 nm) regions may provi...

  19. Changes in selected biochemical parameters in the brain of the fish, Anguilla anguilla (L.), exposed to lindane

    Microsoft Academic Search

    M. D. Ferrando; E. Andreu-Moliner

    1991-01-01

    Pesticides used in pest control programs have been shown to produce many physiological and biochemical changes in freshwater organisms by influencing the activities of several enzymes. Among these physiological changes, energy metabolism has a key role as the animal is forced to expend more energy to mitigate toxic stress. Based on this, an attempt was made to study the sublethal

  20. Interactive effects of photoperiod and light intensity on blood physiological and biochemical reactions of broilers grown to heavy weights

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effects of photoperiod, light intensity, and their interaction on blood acid-base balance, metabolites, and electrolytes in broiler chickens under environmentally controlled conditions were examined in 2 trials. The experiment was consisted of a factorial arrangement of treatments in a randomize...

  1. Sickle cell disease and TCD imaging: inter-hemispheric differences in blood flow Doppler parameters

    PubMed Central

    Krejza, Jaroslaw; Chen, Rong; Romanowicz, Grzegorz; Kwiatkowski, Janet L; Ichord, Rebecca; Arkuszewski, Michal; Zimmerman, Robert; Ohene-Frempong, Kwaku; Desiderio, Lisa; Melhem, Elias R

    2010-01-01

    Purpose To establish reference values of inter-hemispheric differences and ratios of blood flow Doppler parameters in the terminal internal carotid artery (tICA), middle (MCA) and anterior (ACA) cerebral arteries in children with sickle cell anemia. Subjects and Methods Fifty seven out of 74 recruited children (mean age 7.8±3.4 years, 3-14 years), who were free of neurological deficits and intracranial narrowings detectable by magnetic resonance angiography (MRA) and had flow velocities below 170 cm/s by conventional transcranial Doppler ultrasound underwent transcranial color-coded duplex ultrasonography. Reference limits of flow parameters corrected and uncorrected for the angle of insonation were estimated using tolerance intervals, within which are included with probability of 0.90 all possible data values from 95% of a population. Results Reference limits for left-to-right differences in cm/s in the mean angle-corrected and uncorrected flow velocities were: ?56 to 53 and ?72 to 75 for MCA; ?49 to 57 and ?81 to 91 for ACA and ?55 to 64 and ?73 to 78 for tICA, respectively. Respective reference limits for left-to-right velocity ratios were: 0.31 to 1.84 and 0.38 to 1.75 for MCA; 0.48 to 2.99 and 0.46 to 2.89 for ACA, and 0.61 to 2.56 and 0.56 to 2.23 for tICA. Conclusions The study provides reference limits of inter-hemispheric differences and ratios of blood flow Doppler parameters that may be helpful in identification of intracranial arterial narrowings in children with sickle cell disease undergoing ultrasound screening for stroke prevention. PMID:21088242

  2. Parameter estimation and actuator characteristics of hybrid magnetic bearings for axial flow blood pump applications.

    PubMed

    Lim, Tau Meng; Cheng, Shanbao; Chua, Leok Poh

    2009-07-01

    Axial flow blood pumps are generally smaller as compared to centrifugal pumps. This is very beneficial because they can provide better anatomical fit in the chest cavity, as well as lower the risk of infection. This article discusses the design, levitated responses, and parameter estimation of the dynamic characteristics of a compact hybrid magnetic bearing (HMB) system for axial flow blood pump applications. The rotor/impeller of the pump is driven by a three-phase permanent magnet brushless and sensorless motor. It is levitated by two HMBs at both ends in five degree of freedom with proportional-integral-derivative controllers, among which four radial directions are actively controlled and one axial direction is passively controlled. The frequency domain parameter estimation technique with statistical analysis is adopted to validate the stiffness and damping coefficients of the HMB system. A specially designed test rig facilitated the estimation of the bearing's coefficients in air-in both the radial and axial directions. Experimental estimation showed that the dynamic characteristics of the HMB system are dominated by the frequency-dependent stiffness coefficients. By injecting a multifrequency excitation force signal onto the rotor through the HMBs, it is noticed in the experimental results the maximum displacement linear operating range is 20% of the static eccentricity with respect to the rotor and stator gap clearance. The actuator gain was also successfully calibrated and may potentially extend the parameter estimation technique developed in the study of identification and monitoring of the pump's dynamic properties under normal operating conditions with fluid. PMID:19566728

  3. Comparative observations of fever and associated clinical hematological and blood biochemical changes after intravenous administration of staphylococcal enterotoxins B and F (toxic shock syndrome toxin-1) in goats.

    PubMed Central

    Van Miert, A S; Van Duin, C T; Schotman, A J

    1984-01-01

    The present investigation was undertaken to examine the characteristics of purified toxic shock syndrome toxin-1 (staphylococcal enterotoxin F) given intravenously to dwarf goats (dose, 0.02 to 20 micrograms kg-1). Rectal temperature, heart rate, rumen motility, plasma zinc and iron concentrations, and certain other blood biochemical and hematological values were studied and compared with the changes seen after intravenous administration of staphylococcal enterotoxin B (dose, 0.02 to 0.5 micrograms kg-1). Similar changes such as fever, tachycardia, inhibition of rumen contractions, drop in plasma zinc and iron concentrations, lymphopenia, and a decrease in serum alkaline phosphatase activity were observed. In contrast to the effects of toxic shock syndrome toxin-1, staphylococcal enterotoxin B induced colic, watery diarrhea with pseudomembranes, hemoconcentration, and a more pronounced increase in blood urea nitrogen. The results obtained demonstrate that (i) in the goat staphylococcal enterotoxin B is much more potent than toxic shock syndrome toxin-1 and (ii) the goat is a useful model to study the gastro-intestinal effects caused by staphylococcal enterotoxin B. The present finding that no clear relationship could be found between the temperature response and the alterations in zinc and iron levels in plasma support the theory that the febrile reactions and the changes in plasma trace metals are mediated by different polypeptides released by activated macrophages. PMID:6500695

  4. Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat

    PubMed Central

    Sarhan, Osama Mohamed M; Hussein, Rehab M

    2014-01-01

    Background The purpose of this study was to investigate the effect of acute dosing with silver nanoparticles (AgNPs) and identify potential ultrastructural alterations in the liver and kidney and their effect on blood parameters in the albino rat. Methods Twenty rats were used to assess the acute effects of AgNPs. Rats in the treatment group were injected intraperitoneally with 0.5 mL of distilled water containing AgNPs at a dose of 2,000 mg/kg body weight followed by a second injection after 48 hours. Control rats received two 0.5 mL doses of distilled water only. After 3 days, blood samples were collected, and the rat kidneys and livers were extracted and processed for electron microscopy to investigate for hematologic and histopathologic alterations. Results Renal tubules showed swollen epithelium with cytoplasmic vacuolization, thickening of the basement membrane, and destruction of some mitochondrial cristae. Podocytes showed elongation and swelling of their primary and secondary processes. The basement membrane of the capillary tufts became thicker. The hepatic tissue showed narrowing of the sinusoids, swollen hepatocytes with hypertrophied nucleoli, and accumulation of fat globules in the nucleoplasm and cytoplasm. The hepatic sinusoids showed hypertrophied endothelial and Kupffer. Destructed cristae of some mitochondria, endosomes, and larger lysosomes filled with Ag-NPs were also observed in the Kupffer cells. Significant increases were observed in white blood cell count, lymphocyte count, granulocytes, and hemoglobin. There was a significant increase in serum creatinine, urea, and aspartate and alanine aminotransferases. Conclusion To the best of the authors’ knowledge, the ultrastructural changes in renal and liver tissue observed in this study have not been described before. Our results suggest that injection of AgNPs could have severe cytotoxic effects on the structure and function of these organs. PMID:24711700

  5. Relationships between repeated sprint ability, mechanical parameters, and blood metabolites in professional soccer players.

    PubMed

    Morcillo, Jose A; Jiménez-Reyes, Pedro; Cuadrado-Peñafiel, Victor; Lozano, Emilio; Ortega-Becerra, Manuel; Párraga, Juan

    2015-06-01

    Morcillo, JA, Jiménez-Reyes, P, Cuadrado-Peñafiel, V, Lozano, E, Ortega-Becerra, M, and Párraga, J. Relationships between repeated sprint ability, mechanical parameters, and blood metabolites in professional soccer players. J Strength Cond Res 29(6): 1673-1682, 2015-This study analyzed the acute metabolic and mechanical responses to a specific repeated sprint ability (RSA) test. Eighteen male professional soccer players from a team of the First Division of Spanish National League participated. A 12 × 30-m RSA test with 30-second recovery together with countermovement jump test (CMJ) pre a post RSA test was performed. Mechanical responses (i.e., height performance in CMJ and speed loss) and metabolic responses (i.e., blood lactate and ammonia concentrations) were measured before and after exercise. A related sample t-test was used to analyze CMJ height pre-post changes as well as to compare pre- and post-exercise lactate and ammonia levels. Countermovement jump height loss pre-post session (8%) was significant, and fatigue, measured as CMJ height loss, was strongly correlated to lactate (r = 0.97; p < 0.001) and ammonia (r = 0.92; p < 0.001) for all players. The relationships between the variables studied were determined by calculating the Pearson correlation coefficients. The metabolic stress developed during the effort can be estimated by controlling CMJ because of the high correlation between CMJ and blood lactate and ammonia concentrations. The high correlations found between mechanical (speed and CMJ height losses) and metabolic (lactate and ammonia) measures of fatigue highlight the utility and validity of using CMJ to monitor training load and quantify objectively neuromuscular fatigue during RSA. PMID:25463691

  6. Digestibility and blood parameters in the preruminant calf fed a clotting or a nonclotting milk replacer.

    PubMed

    Petit, H V; Ivan, M; Brisson, G J

    1988-04-01

    Eight male Holstein calves 7 to 10 d of age were fed a milk replacer containing a skim milk powder subjected to low-temperature drying either with or without addition of an oxalate-NaOH buffer known to prevent curd formation. The calves were used in a completely randomized design to study the effect of milk clotting on digestibility and blood parameters. Plasma glucose and plasma insulin were similar (P greater than .05) for the clotting and the nonclotting milk replacers. For both treatments, concentrations of glucose and insulin reached a peak 2 h postfeeding (P less than .01). Plasma triglycerides were higher (P less than .01) postfeeding for the nonclotting than for the clotting milk replacer. Plasma essential amino acids and plasma urea were higher, whereas plasma calcium was lower, for the nonclotting milk (P less than .01). Digestibility of dry matter, protein and fat was similar (P greater than .05) between clotting and nonclotting milk. The dry matter content of feces was not affected by clotting (P greater than .05). The data are interpreted to indicate that clotting of the milk replacer modifies blood concentrations of triglycerides, essential amino acids and urea without changing the digestibility of the diet. PMID:3288602

  7. Infleuce of dietary levels of vitamin E and selenium on tissue and blood parameters in pigs.

    PubMed Central

    Young, L G; Lumsden, J H; Lun, A; Claxton, J; Edmeades, D E

    1976-01-01

    Eighteen barrows approximately three weeks of age were used in a 3 X 3 factorial arrangement to investigate the effect of level of supplemental vitamin E and selenium on tissue and blood parameters. Tissue selenium concentrations increased in a quadratic manner with increased selenium intake with kidney tissue containing considerably greater concentrations than liver, heart or muscle. Supplementation of the diet caused a three-fold increase in serum selenium within the first week with a slight tendency to further increases in subsequent weeks. Serum vitamin E of unsupplemented pigs declined by fifty percent during the experiment, whereas supplemental vitamin E resulted in increased serum vitamin E. There was a considerable viration in percent peroxide hemolysis. Correlation of -0.63 between percent peroxide hemolysis and vitamin E intake and -0.85 between percent peroxide hemolysis and serum vitamin E were observed. PMID:1000383

  8. Blood Chemistry of Free-Ranging and Captive White-tailed Deer (Odocoileus virginianus) in Texas 

    E-print Network

    Smith, Melanie Love

    2012-07-16

    Blood samples were collected from 602 white-tailed deer (WTD) (Odocoileus virginianus) between October 2008 – October 2009, from 15 different counties throughout Texas. White-tailed deer were evaluated for serum biochemical parameters (total...

  9. Complete blood count parameters may have a role in diagnosis of gestational trophoblastic disease

    PubMed Central

    Eskicioglu¹, Fatma; Ulkumen, Burcu Artunc; Calik, Esat

    2015-01-01

    Objective: The goal of this study was to investigate whether gestational trophoblastic disease (GTD) and healthy pregnancy differ with respect to complete blood count parameters and these parameters can be used both to explain the pathophysiologic mechanisms and differentiate the two conditions from each other. Methods: The data obtained from 37 women with GTD and 61 healthy pregnancies (control group) regarding platelet (PLT), mean PLT volume (MPV) and PLT distribution width (PDW), and white blood cell (WBC) levels were evaluated. Patients with GTD were further subdivided into two groups composed of 20 partial mole (PM) and 17 complete mole (CM) cases. Results: PDW and WBC were lower in the GTD than the control. There were no differences for PLT and MPV. WBC was lower in PM and both WBC and PDW were lower in CM compared with control. ROC curve analysis revealed an area under curve (AUC) 75.5% for WBC and AUC 69.3% for PDW. A cut-off value was determined 8.19 for WBC with 81.0% sensitivity and 54.1% specificity. While, 15.85 were accepted for PDW, with 87.9% sensitivity and 44.4% specificity. Conclusion: Lower WBC in GTD may suggest that molar pregnancy requires a lower inflammatory reaction facilitating trophoblastic invasion. Lower PDW as an indicator of platelet activation in CM may suggest that CM requires less PLT activation than healthy pregnancy that needs stronger trophoblast invasion for normal placental development. Decreased PDW levels especially < 15.85 and WBC levels < 8.19 may alert clinicians for risk of GTD.

  10. Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials

    Microsoft Academic Search

    Marta Bull; Deborah Lee; Jason Stucky; Ya-Lin Chiu; Abbe Rubin; Helen Horton; M. Juliana McElrath

    2007-01-01

    Interferon-gamma (IFN-?) ELISpot and intracellular cytokine staining (ICS) assays are routinely employed in clinical HIV vaccine trials to identify antigen-specific T cells in cryopreserved peripheral blood mononuclear cells (PBMC). Several parameters involved in blood collection, processing and shipping may influence immunological function of the resulting cells, including anticoagulant type, time from venipuncture to PBMC isolation\\/cryopreservation, method of PBMC isolation and

  11. Restrictive versus liberal blood transfusion policy for hepatectomies in cirrhotic patients

    Microsoft Academic Search

    Masatoshi Makuuchi; Tadatoshi Takayama; Peter Gunvén; Tomoo Kosuge; Susumu Yamazaki; Hiroshi Hasegawa

    1989-01-01

    To evaluate the worth of intra- and postoperative blood transfusion in cirrhotic patients undergoing resection for hepatocellular carcinoma, we compared 13 patients receiving transfusions and 14 matched contemporary patients who did not receive blood. Preoperative hematological and biochemical parameters, the type and extent of liver resection, and the mean blood loss (862 and 870 ml) were similar in the 2

  12. Wounding alters blood chemistry parameters and skin mineralocorticoid receptors in house sparrows (Passer domesticus).

    PubMed

    Lattin, Christine R; DuRant, Sarah E; Romero, L Michael

    2015-06-01

    Because skin is an important physical barrier against pathogens, the ability to quickly and effectively heal wounds directly impacts an animal's health. The hormone corticosterone (CORT) has many complex effects on immune function and can slow wound healing. It has been suggested that CORT's role during wound healing may be to act as a "brake" on inflammation and cell proliferation. This project aimed to clarify the role of CORT in the healing process by quantifying concentrations of its two intracellular receptors, glucocorticoid receptors (GR) and mineralocorticoid receptors (MR), in the skin of wounded (n?=?9) or unwounded (n?=?8) house sparrows (Passer domesticus) using radioligand binding assays. We also quantified GR and MR in liver, spleen and pectoralis muscle, as well as several blood chemistry parameters, hypothesizing that wounding would alter whole-body energy use. One day post-wounding, wounded birds had higher blood glucose and lower aspartate aminotransferase (a marker indicating muscle damage or catabolism) compared to controls, which may be related to animals' changing metabolic needs in response to lymphocyte and macrophage recruitment at the wound site. Birds had significantly decreased MR, but not GR, in the skin of wounded legs compared to the skin of unwounded legs. There was also a trend towards lower MR in wounded skin compared to unwounded birds. Receptors in the three other tissues did not differ between groups. This study suggests that decreasing the skin's sensitivity to CORT immediately after wounding may be a necessary part of the normal healing process in wild birds. J. Exp. Zool. 323A: 322-330, 2015. © 2015 Wiley Periodicals, Inc. PMID:25802121

  13. The rise of blood sugar as an additional parameter in traumatic shock

    Microsoft Academic Search

    D. Holzrichter; L. Meiss; S. Behrens; V. Mickley

    1987-01-01

    Besides the known cardiovascular effects of hemorrhagic shock, regular metabolic consequences can be demonstrated in the rabbit: 1. The rise in blood sugar in hemorrhagic shock increases with the amount of blood withdrawn per unit of time. 2. The characteristic rise in blood sugar lasts longer than the phase of the partial exsanguination. 3. In traumatic shock, the rise in

  14. Survey of selected pathogens and blood parameters of northern yellowstone elk: Wolf sanitation effect implications

    USGS Publications Warehouse

    Barber-Meyer, S. M.; White, P.J.; Mech, L.D.

    2007-01-01

    The restoration or conservation of predators could reduce seroprevalences of certain diseases in prey if predation selectively removes animals exhibiting clinical signs. We assessed disease seroprevalences and blood parameters of 115 adult female elk (Cervus elaphus) wintering on the northern range of Yellowstone National Park [YNP] during 2000-2005 and compared them to data collected prior to wolf (Canis lupus) restoration (WR) in 1995 and to two other herds in Montana to assess this prediction. Blood parameters were generally within two standard deviations of the means observed in other Montana herds (Gravelly-Snowcrest [GS] and Garnet Mountain [GM]), but Yellowstone elk had higher seroprevalences of parainfluenza-3 virus (95% CI YNP = 61.1-78.6, GS = 30.3-46.5) and bovine-virus-diarrhea virus type 1 (95% CI YNP = 15.9-31.9, GM = 0). In comparisons between pre-wolf restoration [pre-WR] (i.e., prior to 1995) seroprevalences with those post-wolf restoration [post-WR] in Yellowstone, we found lower seroprevalences for some disease-causing agents post-wolf restoration (e.g., bovine-virus-diarrhea virus type-1 [95% CI pre-WR = 73.1-86.3, post-WR = 15.9-31.9] and bovine-respiratory syncytial virus [95% CI pre-WR = 70.0-83.8, post-WR = 0]), but similar (e.g., Brucella abortus [95% CI pre-WR = 0-4.45, post-WR = 0-4.74] and epizootic hemorrhagic disease virus [95% CI pre-WR = 0, post-WR = 0]) or higher for others (e.g., Anaplasma marginale [95% CI pre-WR = 0, post-WR = 18.5-38.7] and Leptospira spp. [95% CI pre-WR = 0.5-6.5, post-WR = 9.5-23.5]). Though we did not detect an overall strong predation effect through reduced disease seroprevalence using retrospective comparisons with sparse data, our reference values will facilitate future assessments of this issue.

  15. Laser Doppler monitoring of alterations of blood-flow parameters in fish embryos in response to light irradiation: study of long-term and short-term reactions

    Microsoft Academic Search

    Natalia B. Savchenko; Alexander V. Priezzhev; Pavel V. Revutsky; Borislav A. Levenko

    1997-01-01

    In this paper new experimental results on monitoring of alterations of blood flow parameters in growing fish embryos under laser and non-laser light irradiation are discussed. The measurements were performed by means of laser Doppler technique with high temporal and spatial resolution. Two parameters of blood flows were mostly measured: average velocity and frequency of velocity pulsations. These parameters were

  16. Dissection of the cord blood stromal component reveals predictive parameters for culture outcome.

    PubMed

    Barilani, Mario; Lavazza, Cristiana; Viganò, Mariele; Montemurro, Tiziana; Boldrin, Valentina; Parazzi, Valentina; Montelatici, Elisa; Crosti, Mariacristina; Moro, Monica; Giordano, Rosaria; Lazzari, Lorenza

    2015-01-01

    In regenerative medicine, human cord blood-derived multipotent mesenchymal stromal cells (CBMSCs) stand out for their biological peculiarities demonstrated in in vitro and in vivo preclinical studies. Here, we present our 9-year experience for the consistent isolation of CBMSCs. Although nearly one CB unit out of two retains the potential to give rise to MSC colonies, only 46% of them can be cultured till low passages (P?4), but one-fourth of those reaches even higher passages (P?8). Subsequent characterization for morphological, clonal, differentiation, and proliferation properties revealed two divergent CBMSC behaviors. In particular, a cumulative population doublings cut-off (CPD=15) was identified that undoubtedly distinguishes two growth curves, and different degrees of commitment toward osteogenesis were observed. These data clearly show the existence of at least two distinct CBMSC subsets: one mainly short-living and less proliferative (SL-CBMSCs), the other long-living, with higher growth rate, and, very importantly, with significantly (P?0.01) longer telomere (LL-CBMSCs). Moreover, significant differences in the immunoprofile before seeding were found among CB units giving rise to LL-CBMSCs or SL-CBMSCs or showing no colony formation. Finally, all the aforementioned results provided a peculiar and useful set of parameters potentially predictive for CBMSC culture outcome. PMID:25046283

  17. Misleading biochemical laboratory test results

    PubMed Central

    Nanji, Amin A.

    1984-01-01

    This article reviews the general and specific factors that interfere with the performance of common biochemical laboratory tests and the interpretation of their results. The clinical status of the patient, drug interactions, and in-vivo and in-vitro biochemical interactions and changes may alter the results obtained from biochemical analysis of blood constituents. Failure to recognize invalid laboratory test results may lead to injudicious and dangerous management of patients. PMID:6375845

  18. Effects of different levels of date pits on performance, carcass characteristics and blood parameters of broiler chickens

    Microsoft Academic Search

    A. Masoudi; M. Chaji; M. Bojarpour; Kh. Mirzadeh

    2011-01-01

    The effect of date pits (DP) in diet of broiler chickens on performance, carcass characteristics and blood parameters was studied. A total of 192 day-old commercial broiler chickens were assigned randomly to four experimental diets containing 0, 10, 20, and 30% of DP kg dry matter. All diets were isonitrogenous and isocaloric, and formulated according to nutrient requirements of poultry.

  19. Correlating blood immune parameters and a CCT7 genetic variant with the shedding of Salmonella enterica serovar Typhimurium in swine

    Microsoft Academic Search

    J. J. Uthe; Y. Wang; L. Qu; D. Nettleton; C. K. Tuggle; S. M. D. Bearson

    2009-01-01

    The porcine response to Salmonella infection is critical for control of Salmonella fecal shedding and the establishment of Salmonella carrier status. In this study, 40 crossbred pigs were intranasally inoculated with Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) and monitored for Salmonella fecal shedding and blood immune parameters at 2, 7, 14 and 20 days post-inoculation (dpi). Using a multivariate permutation

  20. Performance, Blood Parameters, and Fecal Egg Counts When Meat Goats Were Finished on Alfalfa, Red Clover, or Orchardgrass Pastures

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the Appalachian Region of the USA, meat goat industries are growing rapidly on small farms to help produce meats for ethnic markets. This experiment was conducted to evaluate weight gain, blood parameters (measurements of nutrient use and anemia resulting from infection with the GI parasite Haem...

  1. Saline or plant-incorporated methylmercury effects on distribution, demethylation, and blood parameters in rats

    Microsoft Academic Search

    M. Czuba; E. Komsta-Szumska; D. C. Mortimer; C. Champagne

    1987-01-01

    The influence of diet is recognized as a significant factor in the expression of toxicity. This applies particularly to toxins like methylmercury (MeHg) which are metabolically incorporated into growing food plants and biotransformed within the plant before ingestion. Methylmercury in this form may influence the early physiological and biochemical events which lead to development of toxicity. In a previous study,

  2. Effect of the treatment with Achyrocline satureioides (free and nanocapsules essential oil) and diminazene aceturate on hematological and biochemical parameters in rats infected by Trypanosoma evansi.

    PubMed

    Do Carmo, Guilherme M; Baldissera, Matheus D; Vaucher, Rodrigo A; Rech, Virginia Cielo; Oliveira, Camila B; Sagrillo, Michele Rorato; Boligon, Aline A; Athayde, Margareth L; Alves, Marta P; França, Raqueli T; Lopes, Sonia T A; Schwertz, Claiton I; Mendes, Ricardo E; Monteiro, Silvia G; Da Silva, Aleksandro S

    2015-02-01

    This study aimed to verify the effect of the treatment with A.?satureioides essential oil (free and nanoencapsulated forms) and diminazene aceturate on hematological and biochemical variables in rats infected by Trypanosoma evansi. The 56 rats were divided into seven groups with eight rats each. Groups A, C and D were composed by uninfected animals, and groups B, E, F and G were formed by infected rats with T.?evansi. Rats from groups A and B were used as negative and positive control, respectively. Rats from the groups C and E were treated with A.?satureioides essential oil, and groups D and F were treated with A.?satureioides nanoencapsulated essential oil. Groups C, D, E and F received one dose of oil (1.5?mL?kg(-1)) during five consecutive days orally. Group G was treated with diminazene aceturate (D.A.) in therapeutic dose (3.5?mg?kg(-1)) in an only dose. The blood samples were collected on day 5 PI for analyses of hematological (erythrocytes and leukocytes count, hemoglobin concentration, hematocrit, mean corpuscular and mean corpuscular hemoglobin concentration) and biochemical (glucose, triglycerides, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin, urea and creatinine) variables. A.?satureioides administered was able to maintain low parasitemia, mainly the nanoencapsulated form, on 5 days post infection. On the infected animals with T.?evansi treated with A.?satureioides essential oil (free and nanocapsules) the number of total leucocytes, lymphocytes and monocytes present was similar to uninfected rats, and different from infected and not-treated animals (leukocytosis). Treatment with A.?satureioides in free form elevated levels of ALT and AST, demonstrating liver damage; however, treatment with nanoencapsulated form did not cause elevation of these enzymes. Finally, treatments inhibited the increase in creatinine levels caused by infection for T.?evansi. In summary, the nanoencapsulated form showed better activity on the trypanosome; it did not cause liver toxicity and prevented renal damage. PMID:25499512

  3. Spatial Distribution of Biochemical Parameters Indicating Biomass and Community Composition of Microbial Assemblies In Estuarine Mud Flat Sediments

    PubMed Central

    Federle, Thomas W.; Hullar, Meredith A.; Livingston, Robert J.; Meeter, Duane A.; White, David C.

    1983-01-01

    The spatial distribution of communities was examined in estuarine mud flat sediments by the biochemical analysis of the lipids and lipid components extracted from the sediments. Total phospholipid was used as a measure of total biomass, and fatty acids were used as indicators of community composition. Comparisons were made among 2- by 2-m (location) and 0.2- by 0.2-m (cluster) sampling plots by using a nested analysis of variance to design an optimal sampling strategy to define the microbial content of a large, relatively homogenous area. At two of the three stations, a 2- by 2-m plot was representative of the station, but 0.2- by 0.2-m areas were in no case representative of the station. The biomass measured by the extractable phospholipid and the total lipid palmitic acid showed excellent correlation with the fatty acid “signatures” characteristic of bacteria, but showed a lower correlation with the long-chain polyenoic fatty acids characteristic of the microfauna. PMID:16346181

  4. Genetic parameters and correlations of collar rot resistance with important biochemical and yield traits in opium poppy (Papaver somniferum L.).

    PubMed

    Trivedi, Mala; Tiwari, Rajesh K; Dhawan, Om P

    2006-01-01

    Collar rot, caused by Rhizoctonia solani Kühn, is one of the most severe fungal diseases of opium poppy. In this study, heritability, genetic advance and correlation for 10 agronomic, 1 physiological, 3 biochemical and 1 chemical traits with disease severity index (DSI) for collar rot were assessed in 35 accessions of opium poppy. Most of the economically important characters, like seed and capsule straw yield per plant, oil and protein content of seeds, peroxidase activity in leaves, morphine content of capsule straw and DSI for collar rot showed high heritability as well as genetic advance. Highly significant negative correlation between DSI and seed yield clearly shows that as the disease progresses in plants, seed yield declines, chiefly due to premature death of infected plants as well as low seed and capsule setting in the survived population of susceptible plants. Similarly, a highly significant negative correlation between peroxidase activity and DSI indicated that marker-assisted selection of disease-resistant plants based on high peroxidase activity would be effective and survived susceptible plants could be removed from the population to stop further spread. PMID:16424606

  5. Hematologic and plasma biochemical reference values in Indian peafowl (Pavo cristatus).

    PubMed

    Samour, Jaime; Naldo, Jesus; Rahman, Habeeb; Sakkir, Mohammed

    2010-06-01

    Blood samples were collected from captive, adult, clinically normal Indian peafowl (Pavo cristatus) for hematologic and plasma biochemical analyses. Hematologic parameters investigated were total red blood cell count, hemoglobin, packed cell volume, fibrinogen, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, total white blood cell count, differential white blood cell count, and thrombocyte count. Plasma biochemical parameters investigated were alanine aminotransferase, alkaline phosphatase, amylase, aspartate aminotransferase, bile acids, total bilirubin, blood urea nitrogen, calcium, cholesterol, creatinine, creatine kinase, gamma glutamyltransferase, lactate dehydrogenase, glucose, iron, phosphorus, and uric acid, as well as plasma protein electrophoresis. Results were compared with values from studies done in houbara bustards (Chlamydotis undulata), kori bustards (Ardeotis kori), stone curlews (Burhinus oedicnemus), and taxonomically related species, including ring-necked pheasants (Phasianus colchicus), red-legged partridges (Alectoris rufa), Kashmir native fowl (Kashmirfavorella), and Bangladesh native, Fayoumi, and Assil fowl (Gallus domesticus). PMID:20806654

  6. Gill Tissue Recovery after Copper Exposure and Blood Parameter Responses in the Tropical Fish Prochilodus scrofa

    Microsoft Academic Search

    Carla C. C. Cerqueira; Marisa N. Fernandes

    2002-01-01

    Changes in Prochilodus scrofa gill tissue and in blood responses were investigated after 96-h copper exposure and transference to clean water. Gill damage was characterized by epithelial lifting, cell swelling, pavement, chloride and mucous cell proliferation, and blood vessel anomalies. Restoration of gill structure was slow, with no tissue improvements in the first 2 days in clean water. From the

  7. EFFECTS OF LOW LEVEL CARBON MONOXIDE EXPOSURE. BLOOD LIPIDS AND COAGULATION PARAMETERS

    EPA Science Inventory

    The study examined the effects of carbon monoxide (CO) in 50 and 100 ppm doses on response to treadmill exercise, blood coagulation and blood lipids in normal men. Twenty-three men were exposed to CO or to air in a double-blind protocol. After exposure, each underwent a graded ex...

  8. Tomographic gated blood pool studies: The parameters for collection and reconstruction

    SciTech Connect

    Doherty, P.W.; King, M.A.; Schwinger, R.B.

    1984-01-01

    Because of the acquisition time and data processing load problems associated with the collection and analysis of multiple gated views required to obtain cine tomographic sections, the authors attempted to document the minimum data requirement for a high quality study. An idealized study (64 views over 360/sup 0/, 32 frames per R-R interval) with an ultra high resolution collimator, and a 20% asymmetric energy window was obtained and analyzed using multiple combinations of views, frames per view, and total count. Optimal image quality was maintained while using 16 views over 180/sup 0/ (RAO 45/sup 0/ to LPO 33.75/sup 0/), 16 frames per view, and an acquisition time of 2 minutes per view which with a 25 mCi dose of Tc-99m provided approximately 10/sup 6/ counts per view. These were the parameters utilized in subsequent patient studies. Using several different types of pre and post reconstruction filtering, optimum image quality was obtained with Metz spatial and low-pass temporal domain prefiltering and a ramp reconstruction filter. As an alternative to cine display, functional images of the transverse sections based on pixel by pixel time activity curves were generated. These included ejection fraction, phase, and maximum positive and negative first derivative image sets. The overall processing time for such studies is 20 minutes with the aid of an array processor, with a patient through-put time of 45 minutes. The authors conclude that good quality gated blood pool studies can be acquired in a clinically acceptable time frame. However, there persists a significant problem with data storage, handling and image interpretation.

  9. The clinical spectrum of renal osteodystrophy in 57 chronic hemodialysis patients: a correlation between biochemical parameters and bone pathology findings.

    PubMed

    Chazan, J A; Libbey, N P; London, M R; Pono, L; Abuelo, J G

    1991-02-01

    Fifty-nine chronic hemodialysis patients who had been on dialysis for an average of 77 months underwent bone biopsies and the pathologic findings were correlated with biochemical and demographic data. All but two had evidence of renal osteodystrophy, 23 with osteitis fibrosa (OF), 19 with osteomalacia and/or adynamic disease (OM/AD), and 15 with mixed osteodystrophy (MOD). Patients in each group were similar with regard to age, sex distribution, duration of dialysis, unstimulated serum aluminum, calcium and phosphorus. Patients with osteitis fibrosa (OF) had statistically higher DFO stimulated aluminum, alkaline phosphatase and PTHC levels than the other two groups although there was marked individual variation. The bone biopsies were also evaluated for the amount of aluminum deposited in the osteoid seam. All 23 of the patients with OF and 11 of the 15 patients with MOD had no, mild, or minimal aluminum deposition but 12 of the 19 patients with OM/AD had moderate to marked aluminum deposition. Patients with minimal to mild aluminum deposition were similar in age, duration of dialysis, sex distribution, unstimulated and DFO stimulated aluminum levels, calcium, phosphorus, alkaline phosphatase to those with moderate to marked deposition but had significantly higher parathormone levels. All patients had been treated in a similar fashion regarding diet, oral phosphate binders and vitamin D; therefore, the observed differences in bone pathology were not readily explicable. However, patients who were found to have osteitis fibrosa and those with minimal to mild aluminum deposition had significantly higher parathormone levels when compared with patients in the other groups at the inception of dialysis. PMID:2019018

  10. Bed bugs, their blood sources and life history parameters: a comparison of artificial and natural feeding.

    PubMed

    Aak, A; Rukke, B A

    2014-03-01

    A blood-feeding system that utilizes a small amount of whole heparinized human blood in parafilm bags is described in detail, and similarities and differences between artificially fed and naturally rodent-fed bed bugs (Hemiptera: Cimicidae) are discussed. Blood with high levels of heparin (10%) was unsuitable for artificial colony rearing, whereas bed bugs fed on 1% heparinized blood and those that naturally ingested rat blood completed their lifecycle with similar stage structures over time, with no significant differences in mortality. No differences in feeding efficiency or fertility were found in a direct comparison of bed bugs maintained under each of these two treatments, but analysis of the full lifecycle revealed that artificially fed bed bugs became significantly smaller and laid fewer eggs than rodent-fed bed bugs. The level of membrane stretching regulated the number of bed bugs that fed. When the membrane was stretched to twice its length and width, 96% of bed bugs successfully fed through the parafilm. Whole heparinized blood that was stored at 6?°C for ??14?days failed to retain its nutritional value and the amount of blood consumed and number of consecutive moults were significantly reduced. PMID:23692154

  11. Association of Renal Biochemical Parameters with Left Ventricular Diastolic Dysfunction in a Community-Based Elderly Population in China: A Cross-Sectional Study

    PubMed Central

    Zhou, Jun; Zhang, Hanying; Tang, Bixiao; Fu, Michael; Herlitz, Hans; Cui, Jie; Zhu, Hongmin; Sun, Aijun; Hu, Kai; Ge, Junbo

    2014-01-01

    Background Relationship of left ventricular diastolic dysfunction (LVDD) with parameters that could provide more information than hemodynamic renal indexes has not been clarified. We aimed to explore the association of comprehensive renal parameters with LVDD in a community-based elderly population. Methods 1,166 community residents (aged ? 65 years, 694 females) participating in the Shanghai Heart Health Study with complete data of renal parameters were investigated. Echocardiography was used to evaluate diastolic function with conventional and tissue Doppler imaging techniques. Serum urea, creatinine, urea-to-creatinine ratio, estimated glomerular filtration rate (eGFR) and urinary albumin-to-creatinine ratio (UACR) were analyzed on their associations with LVDD. Results The prevalence of LVDD increased in proportion to increasing serum urea, urea-to-creatinine ratio and UACR. These three renal parameters were found negatively correlated to peak early (E) to late (A) diastolic velocities ratio (E/A), and positively to left atrial volume index; UACR also positively correlated with E to peak early (E’) diastolic mitral annular velocity ratio (E/E’). Serum urea, urea-to-creatinine ratio and UACR correlated with LVDD in logistic univariate regression analysis, and urea-to-creatinine ratio remained independently correlated to LVDD [Odds ratio (OR) 2.82, 95% confidence interval (CI) 1.34–5.95] after adjustment. Serum urea (OR 1.18, 95%CI 1.03–1.34), creatinine (OR 6.53, 95%CI 1.70­–25.02), eGFR (OR 0.22, 95%CI 0.07–0.65) and UACR (OR 2.15, 95%CI 1.42–3.24) were revealed independent correlates of advanced (moderate and severe) LVDD. Conclusions Biochemical parameters of renal function were closely linked with LVDD. This finding described new cardio-renal relationship in the elderly population. PMID:24533126

  12. Effect of Coriolus versicolor polysaccharides on the hematological and biochemical parameters and protection against Aeromonas hydrophila in allogynogenetic crucian carp (Carassius auratus gibelio).

    PubMed

    Wu, Zhi-xin; Pang, Su-feng; Chen, Xiao-xuan; Yu, Yan-mei; Zhou, Jin-min; Chen, Xi; Pang, Li-jiao

    2013-04-01

    The effect of dietary intake of Coriolus versicolor Polysaccharides (CVP) on the hematological and biochemical indices of Allogynogenetic crucian carp (Carassius auratus gibelio) was investigated. Fish were fed CVP supplemented diets (0, 0.25, 0.5, 1.0, 2.0 or 4.0 g CVP kg(-1)) for 56 days. The RBC, WBC counts, hemoglobin content, ESR in blood and TP, ALT, AST, ALP, GLU, CHO, TG, and BUN in serum were measured on day 0, 14, 28, 42, and 56. After feeding of 56 days, fish were infected with Aeromonas hydrophila and mortalities were recorded. The results indicated that feeding crucian carp with suitable dose of CVP enhanced the RBC, WBC counts, hemoglobin and TP content, ALP activity, and decreased the ESR, ALT, AST, GLU, CHO, TG and BUN. There was no effect in fish at low dose (0.25 g kg(-1)). Unexpectedly, the higher CVP dose used here (2.0 and 4.0 g kg(-1)) has a negative effect in fish. The results of challenge experiment indicated that a moderate level of CVP in the diet (1.0 g kg(-1)) was the most effective to enhance the survival of fish after infected with A. hydrophila. In summary, the use of CVP, as dietary supplements, can improve the innate defense of crucian carp providing resistance to pathogens. PMID:22791194

  13. [Physical, biochemical and bacterioscopic parameters of mouth lavage in patients with periodontium diseases and working on ethanol production enterprises].

    PubMed

    Bandrivskaia, N N; Mrochko, O I; Bandrivski?, Iu L

    2014-01-01

    The parameters of oral bacterioscopy and homeostasis were studied in patients with periodontal diseases. The shift of pH towards the acid values, increased content of total protein were determined. Analysis of oral fluid showed increase in level of ionized calcium, nitrate anion, and decreased content of magnesium and ammonium cations. The increased overall number of bacteria in patients with periodontal diseases was seen, especially due to gram-negative bacteria. PMID:25335425

  14. Effect of Harsh Environmental Conditions on Nutrient Utilization and Blood Parameters of Awassi Sheep and Kilis Goat Fed Different Levels of Concentrate Feed

    Microsoft Academic Search

    A. Can; N. Denek; M. ?eker

    2008-01-01

    Can, A., Denek, N. and ?eker, M. 2008. Effect of harsh environmental conditions on nutrient utilization and blood parameters of Awassi sheep and Kilis goat fed different levels of concentrate feed. J. Appl. Anim. Res., 33: 39–43.To determine the season effect (summer vs winter) on nutrient utilization and blood, rumen parameters of Awassi ram lambs and male Kilis goats fed

  15. Biochemical, photosynthetic and productive parameters of Chinese cabbage grown under blue-red LED assembly designed for space agriculture

    NASA Astrophysics Data System (ADS)

    Avercheva, Olga; Berkovich, Yuliy A.; Smolyanina, Svetlana; Bassarskaya, Elizaveta; Pogosyan, Sergey; Ptushenko, Vasiliy; Erokhin, Alexei; Zhigalova, Tatiana

    2014-06-01

    Currently light emitting diodes (LEDs) are considered to be most preferable source for space plant growth facilities. We performed a complex study of growth and photosynthesis in Chinese cabbage plants (Brassica chinensis L.) grown with continuous LED lighting based on red (650 nm) and blue (470 nm) LEDs with a red to blue photon ratio of 7:1. Plants grown with high-pressure sodium (HPS) lamps were used as a control. PPF levels used were about 100 ?mol/(m2 s) (PPF 100) and nearly 400 ?mol/(m2 s) (PPF 400). One group of plants was grown with PPF 100 and transferred to PPF 400 at the age of 12 days. Plants were studied at the age of 15 and 28 days (harvest age); some plants were left to naturally end their life cycle. We studied a number of parameters reflecting different stages of photosynthesis: photosynthetic pigment content; chlorophyll fluorescence parameters (photosystem II quantum yield, photochemical and non-photochemical chlorophyll fluorescence quenching); electron transport rate, proton gradient on thylakoid membranes (?pH), and photophosphorylation rate in isolated chloroplasts. We also tested parameters reflecting plant growth and productivity: shoot and root fresh and dry weight, sugar content and ascorbic acid content in shoots. Our results had shown that at PPF 100, plants grown with LEDs did not differ from control plants in shoot fresh weight, but showed substantial differences in photophosphorylation rate and sugar content. Differences observed in plants grown with PPF 100 become more pronounced in plants grown with PPF 400. Most parameters characterizing the plant photosynthetic performance, such as photosynthetic pigment content, electron transport rate, and ?pH did not react strongly to light spectrum. Photophosphorylation rate differed strongly in plants grown with different spectrum and PPF level, but did not always reflect final plant yield. Results of the present work suggest that narrow-band LED lighting caused changes in Chinese cabbage plants on levels of the photosynthetic apparatus and the whole plant, concerning its development and adaptation to a varying PPF level.

  16. The Effect of Age on Hematological and Serum Biochemical Values on Juvenile Ring-Necked Pheasants (Phasianus colchicus)

    Microsoft Academic Search

    Elizabeth Moreira dos Santos Schmidt; Antonio Carlos Paulillo; Rosangela Locatelli Dittrich; Elizabeth Santin; Paula Cristina Linder da Silva; Olair Beltrame; Edson Goncalves de Oliveira

    2007-01-01

    1 5 Abstract: The influences of age on hematological and serum biochemical parameters were investigated in juvenile ring-necked pheasant. Statistical comparisons were made for differences in values among different ages. A variety of blood parameters showed significantly age related differences. Juvenile ring- necked pheasants of all ages had lymphocytes as the major circulating leukocyte. RBC values for 60 day-old pheasants

  17. A Clinical and Epidemiological Study of Psoriasis and its Association with Various Biochemical Parameters in Newly Diagnosed Cases

    PubMed Central

    M.G., Gopal; Talwar, Ankur; Kumar B.C., Sharath; M., Ramesh; A.S., Nandini; H.B., Meena

    2013-01-01

    Background: There has been a lot of recent search on consideration of psoriasis as a systemic disease, with researchers being of the view that dermatological manifestations represent only a part of the spectrum. Although, there have been plenty of studies from the west reporting an association of psoriasis with the metabolic syndrome, there are no large-scale Indian studies evaluating Asian patients. The present study is an endeavour in this regard. Aim: To investigate the prevalence of metabolic syndrome in Indian patients with newly diagnosed psoriasis at the onset of the disease. Methods: The study is a prospective clinical case control study, with 100 patients of psoriasis and 100 age-matched healthy controls. 5ml plain venous blood after overnight fasting was obtained by venepuncture. Plasma glucose was tested by glucose oxidase method. Serum cholesterol and triglycerides was estimated by enzymatic method. Metabolic syndrome was diagnosed by the presence of three or more criterion of the National Cholestrol Education Programme’s Adult Panel (ATP). The statistical software SAS 9.2 and SPSS 15.0 was used for the analysis of the data. Results: Metabolic syndrome was diagnosed in 8 out of 100 cases and 9 out of 100 controls (p-value: 0.811). We did not find any association of psoriasis with metabolic syndrome in our study. The age of onset of the disease, the duration of the disease and the severity of the disease activity were also not found to be associated with the likelihood of developing metabolic syndrome. Conclusion: Our study refuted any association of psoriasis with metabolic syndrome at the onset of disease activity in Indian patients. The plenty of reports from west approving such an association can be explained by increased risk factors like smoking, alcohol consumption, obesity and stress levels. Further, most such studies have been conducted with patients on treatment, while ours is the first study on newly diagnosed patients prior to the initiation of any therapy. PMID:24551670

  18. Effect of Ramadan Fasting on Body Weight, (BP) and Biochemical Parameters in Middle Aged Hypertensive Subjects: An Observational Trial

    PubMed Central

    M, Salahuddin; AH, Sayed Ashfak; SR, Syed; KM, Badaam

    2014-01-01

    Introduction: Ramadan fasting is a religious obligation which is practised by Muslim population all over the world. However, there is scarcity of scientific literature regarding its effects on health determinants in cardiovascular disturbances like hypertension. Objects:The present study was done to assess the (BP), body weight and serum cholesterol changes over the period of Ramadan fasting in patients with hypertension. Materails And Methods:This prospective observational trial was done on 15 hypertensive subjects who were in the age group of 35 to 65 years, who were determined to complete Ramadan fast. All subjects were on antihypertensive therapy. Outcome measures of (BP), body weight and serum cholesterol were assessed in all the subjects before and after Ramadan month. Results: Mean age of subjects was 44.6 ± 5.62 years. Systolic BP decreased from 148 ± 19.6 to 132.5 ± 17.9 mm of Hg. The decrease of 15.5 units (95% CI: 7.5 to 24.4) was statistically significant (p = 0.0009). Diastolic BP decreased from 90.4 ± 7.8 to 81.1 ± 6.3 mm of Hg. The decrease of 9.3 units (95% CI: 5.7 to 13) was statistically significant (p < 0.0001). There was statistically significant decrease in body weight from 66.6 ± 13 to 65.2 ± 12.7 kg (p < 0.0001). There was no significant difference in serum cholesterol from 187.3 ± 28.9 to 192.7 ± 31.3 mg% (p = 0.37). Conclusion: Hypertensive patients with continuation of their medicines showed a decrease in blood pressure and reduction in body weight at the end of Ramadan fasting duration. However there was no change found in serum cholesterol levels. PMID:24783068

  19. Association between umbilical blood gas parameters and neonatal morbidity and death in neonates with pathologic fetal acidemia

    Microsoft Academic Search

    R. L. Andres; G. Saade; L. C. Gilstrap; I. Wilkins; A. Witlin; F. Zlatnik; G. V. Hankins

    1999-01-01

    Objective: Our purpose was to correlate umbilical artery blood gas parameters with neonatal death and indicators of morbidity in neonates with pathologic fetal acidemia (pH <7.0). Study Design: We reviewed maternal and neonatal charts of 93 neonates with an umbilical artery pH <7.0 who were delivered at 2 university-based centers. The relationships between umbilical artery pH, PO2, PCO2, bicarbonate, base

  20. Rose hip inhibits chemotaxis and chemiluminescence of human peripheral blood neutrophils in vitro and reduces certain inflammatory parameters in vivo

    Microsoft Academic Search

    Arsalan Kharazmi; Kaj Winther

    1999-01-01

    Objective and Design  The objective of this study was to investigate the leucocyte-related antiinflammatory properties of rose hip. Materials and\\u000a Methods: The effect of rose hip on a number of inflammatory parameters was evaluated using the following models: (1) The effect\\u000a of rose hip extract on chemotaxis and chemiluminescence of peripheral blood polymorphonuclear leucocytes (PMNs) from healthy\\u000a subjects in vitro; (2)

  1. Effects of Supplemental Yeast (Saccharomyces cerevisiae) Culture on Rumen Development, Growth Characteristics, and Blood Parameters in Neonatal Dairy Calves

    Microsoft Academic Search

    K. E. Lesmeister; A. J. Heinrichs; M. T. Gabler

    2004-01-01

    Yeast (Saccharomyces cerevisiae) culture was added to a texturized calf starter at 0 (control), 1, or 2% of dry matter todetermine effects on intake,growth, blood parameters,and rumendevelopment. Seventy-fiveHol- stein calves (38 male; 37 female) were started on the experiment at 2 ± 1 d of age and were studied for 42 d. Starter intake was measured, and fecal scoring was

  2. Physiological and biochemical parameters for identification of QTLs controlling the winter triticale drought tolerance at the seedling stage.

    PubMed

    Hura, Tomasz; Hura, Katarzyna; Grzesiak, Stanis?aw

    2009-03-01

    The genetic map of the triticale is created on the basis of double-haploid (DH) lines, derived from F1 hybrids of a cross between the parental line Saka3006 and Modus. In order to localise drought resistance genes, it is necessary to find a phenotype feature which clearly differentiates between parental lines under drought stress conditions. With the future in mind, the aim of the presented studies was to analyse differences in the response to drought stress, between Saka3006 and Modus. Analyses of the water status of leaves, and the activity of the photosynthetic apparatus and protective mechanisms relating to the accumulation of phenolic compounds, were carried out. The studies were completed during the tillering phase. Statistically significant changes, between genotypes experiencing the drought period, were noticed for the osmotic potential, leaf water content, some parameters of chlorophyll fluorescence, and for phenolics and the ferulic acid content. On the basis of the studies, the Saka genotype can be considered drought resistant due to higher leaf water content caused, probably, by smaller hydraulic resistance relative to Modus. The activity of its photosynthetic apparatus during drought was higher than that for the Modus genotype. The high level of phenolic compounds, which can act as photoprotectors and free radical scavengers, was also maintained. All the mentioned parameters can represent the potential phenotype features, which allow the identification of resistance genes on the genetic map of the triticale, which is currently being created. PMID:19059788

  3. Effects of Different Levels of Coriander (Coriandrum sativum) Seed Powder and Extract on Serum Biochemical Parameters, Microbiota, and Immunity in Broiler Chicks

    PubMed Central

    Hosseinzadeh, Hesam; Alaw Qotbi, Ali Ahmad; Seidavi, Alireza; Norris, David; Brown, David

    2014-01-01

    The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28?d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds. PMID:25614892

  4. BIOCHEMICAL CHANGES IN HUMANS UPON EXPOSURE TO SULFURIC ACID AEROSOL AND EXERCISE

    EPA Science Inventory

    A total of 18 human subjects were exposed to ambient air for four hours on the first day of exposure and to four hours of 100 micrograms/cu. m. (0.033 micromole) sulfuric acid aerosol exposed to four hours of ambient air on both exposure days. Six biochemical blood parameters wer...

  5. BIOCHEMICAL EFFECTS OF SULFURIC ACID MIST INHALATION BY HUMAN SUBJECTS WHILE AT REST

    EPA Science Inventory

    The study evaluated the effect of sulfuric acid aerosol exposure for 2 consecutive days on seven human biochemical blood parameters. A total of 20 human subjects were exposed to 100 micrograms per cu. m. sulfuric acid aerosol for 4 hr/day for 2 consecutive days. A total of 17 hum...

  6. Variations of biochemical parameters along a transect in the Southern Ocean, with special emphasis on volatile halogenated organic compounds

    NASA Astrophysics Data System (ADS)

    Abrahamsson, K.; Bertilsson, S.; Chierici, M.; Fransson, A.; Froneman, P. W.; Lorén, A.; Pakhomov, E. A.

    2004-11-01

    A number of parameters of biogeochemical interest were monitored along a north-southerly transect (S 43-S 63°) in the Atlantic Sector of the Southern Ocean from the 8th to the 20th of December 1997. Changes in total dissolved inorganic carbon ( CT) and total alkalinity ( AT) were mostly dependent on temperature and salinity until the ice edge was reached. After this point only a weak correlation was seen between these. Highest mean values of CT and AT were observed in the Winter Ice Edge (WIE) (2195 and 2319 ?mol kg -1, respectively). Lowest mean AT (2277 ?mol kg -1) was observed in the Sub-Antarctic Front (SAF), whereas lowest mean CT concentration (2068 ?mol kg -1) was associated with the Sub-Tropical Front (STF). The pH in situ varied between 8.060 and 8.156 where the highest values were observed in the southern part of the Antarctic Polar Front (APF) and in the Summer Ice Edge (SIE) Region . These peaks were associated with areas of high chlorophyll a (chl a) and tribromomethane values. In the other areas the pH in situ was mainly dependent on hydrography. Bacterial abundance decreased more than one order of magnitude when going from north to south. The decrease appeared to be strongly related to water temperature and there were no elevated abundances at frontal zones. Microphytoplankton dominated in the SAF and APF, whereas the nano- and picoplankton dominated outside these regions. Volatile halogenated compounds were found to vary both with regions, and with daylight. For the iodinated compounds, the highest concentrations were found north of the STF. Brominated hydrocarbons had high concentrations in the STF, but elevated concentrations were also found in the APF and SIE regions. No obvious correlation could be found between the occurrence of individual halocarbons and chl a. On some occasions trichloroethene and tribromomethane related to the presence of nano- and microplankton, respectively.

  7. Assessment of clinical biochemical parameters in Roma minority residing in eastern Slovakia compared with the majority population.

    PubMed

    Hubková, Beáta; Maslanková, Jana; Stupák, Marek; Guzy, Juraj; Kovácová, Anna; Pella, Daniel; Jarcuska, Peter; Mareková, Mária

    2014-03-01

    Roma constitute the largest ethnic minority in Europe and the second largest minority in Slovakia. Their health problems originate mainly from their low socioeconomic status, certain cultural aspects and their health-threatening lifestyle as well as the psycho-social burden arising from poverty and frequent migration. Evaluation of glucose, albumin, triacylglycerol (TAG) and low density lipoprotein cholesterol (LDL-C) concentrations did not reveal any clue about the presumed deteriorated health of the Roma population. Higher proportions of subjects with elevated serum total cholesterol were found in Roma women as compared to both control groups of women (p = 0.027, p = 0.006) and in Roma men as compared to the male control group living in standard conditions. Only the low level of HDL-cholesterol gives a glimpse of their deteriorated health. Significantly lower levels of serum HDL-C were reported in Roma men and women compared to the respondents in both control groups with a p value of p < 0.001. Comparing the ratio of LDL-C/HDL-C yielded significant differences between the number of physiological values in Roma men and men from the control group 1 (p = 0.022) in favour of the control group. When comparing the number of people with physiological values of cholesterols and with worsening TAG parameters at the same time, the increased risk of Roma men compared with men from the control group 1 became evident, with a level of significance of p = 0.023. Evaluation of urine samples pointed to significantly higher concentrations of urinary protein in Roma women compared with women in the control group 1 (p = 0.012). PMID:24847608

  8. Comparison of Total Antioxidant Capacity Oxidative Stress and Blood Lipoprotein Parameters in Volleyball Players and Sedentary

    ERIC Educational Resources Information Center

    Gokhan, Ismail

    2013-01-01

    This study aims to measure, then compare sedentary blood lipoproteins, oxidant- antioxidant state and oxidative stress index in volleyball players. The experimental group of the research consists of regularly practising 20 boys between the ages of 12 and 17, and the control group comprises 32 children practising no particular sports branch, 12 of…

  9. Statistical Analysis of Blood Parameter Surveillance Data from Workers at an Organotin Production Facility

    Microsoft Academic Search

    Channing R. Meyer; C. Ralph Buncher; Richard Gioscia; Janice Dees

    1987-01-01

    Biological surveillance data was analyzed at a plant which produced organic and inorganic tin chemicals. No chronic health problems related to occupational exposures were detected by biological testing history or physical examination. A statistical evaluation of biological values was conducted in an attempt to detect any significant changes. The blood values of all workers were well within normal limits. Workers

  10. Evaluation of Blood Pressure Control using a New Arterial Stiffness Parameter, Cardio-ankle Vascular Index (CAVI)

    PubMed Central

    Shirai, Kohji; Utino, Junji; Saiki, Atsuhito; Endo, Kei; Ohira, Masahiro; Nagayama, Daiji; Tatsuno, Ichiro; Shimizu, Kazuhiro; Takahashi, Mao; Takahara, Akira

    2013-01-01

    Arterial stiffness has been known to be a surrogate marker of arteriosclerosis, and also of vascular function. Pulse wave velocity (PWV) had been the most popular index and was known to be a predictor of cardiovascular events. But, it depends on blood pressure at measuring time. To overcome this problem, cardio-ankle vascular index (CAVI) is developed. CAVI is derived from stiffness parameter ? by Hayashi, and the equation of Bramwell-Hill, and is independent from blood pressure at a measuring time. Then, CAVI might reflect the proper change of arterial wall by antihypertensive agents. CAVI shows high value with aging and in many arteriosclerotic diseases and is also high in persons with main coronary risk factors. Furthermore, CAVI is decreased by an administration of ?1 blocker, doxazosin for 2-4 hours, Those results suggested that CAVI reflected the arterial stiffness composed of organic components and of smooth muscle cell contracture. Angiotensin II receptor blocker, olmesartan decreased CAVI much more than that of calcium channel antagonist, amlodipine, even though the rates of decreased blood pressure were almost same. CAVI might differentiate the blood pressure-lowering agents from the point of the effects on proper arterial stiffness. This paper reviewed the principle and rationale of CAVI, and the possibilities of clinical applications, especially in the studies of hypertension. PMID:23807874

  11. Correlations between cerebral blood flow variations and clinical parameters in temporal lobe epilepsy: an interictal study.

    PubMed Central

    Valmier, J; Touchon, J; Daures, P; Zanca, M; Baldy-Moulinier, M

    1987-01-01

    Regional cerebral blood flow (rCBF) measurements were determined by the intravenous Xenon 133 technique in 80 patients suffering from temporal lobe epilepsy. All the patients had a normal CT scan. Three subgroups were differentiated, according to EEG and all-night polygraphic recordings: temporal lobe epilepsy with left (N = 25) or right (N = 25) EEG epileptic abnormalities and temporal lobe epilepsy with EEG abnormalities in both temporal regions with asynchronous occurrence (n = 30). In comparison with a control group (n = 20), there was (1) a marked reduction of blood flow in the temporal region corresponding to the site of the epileptic focus and (2) a reduction in blood flow in distant brain areas and the contralateral hemisphere. The rCBF decrease was highly correlated (p less than 0.001) with the disease severity (taking into account the complex partial seizure frequency and the number of secondary generalised seizures). Differences were found in the rCBF decrease between left and right temporal lobe epilepsy. PMID:3681308

  12. Non-invasive Studies on Age Related Parameters Using a Blood Volume Pulse Sensor

    Microsoft Academic Search

    V. K. Jayasree; T. V. Sandhya; P. Radhakrishnan

    2008-01-01

    A non-invasive technique is implemented to measure a parameter which is closely related to the distensibility of large arteries, using the second derivative of the infrared photoplethysmographic waveform. Thirty subjects within the age group of 20-61 years were involved in this pilot study. Two new parameters, namely the area of the photoplethysmographic waveform under the systolic peak, and the ratio

  13. Role of sudarshan kriya and pranayam on lipid profile and blood cell parameters during exam stress: A randomized controlled trial

    PubMed Central

    Subramanian, Swapna; Elango, Tamilselvi; Malligarjunan, Hemamalini; Kochupillai, Vinod; Dayalan, Haripriya

    2012-01-01

    Background: Yoga is a science practiced in India over thousands of years. It produces constituent physiological changes and has sound scientific basis. Aim: Since exam stress modifies lipid profile and hematological parameters, we conducted an investigation on the effect of sudarshan kriya (SK and P) program on these parameters. Materials and Methods: Blood samples of 43 engineering students were collected at four intervals namely baseline (BL), exam stress (ES), three and six weeks practice of SK and P during exam stress. Lipid profile and hematological parameters were measured at all four intervals. Results: ES elevated total cholesterol (TC), triglycerides (TGL) and very low density lipoprotein (VLDL) levels. Hematological parameters affected by ES included neutrophil, lymphocytes, platelet count, packed cell volume (PCV) and mean cell volume (MCV). Three and six weeks practice of SK and P reduced the elevated lipid profile, hematological parameters and improved lymphocyte levels. Conclusion: Our study indicates that SK and P practice has the potential to overcome ES by improving lipid profile and hematological parameters. PMID:22346062

  14. Impact of tannic acid on blood pressure, oxidative stress and urinary parameters in L-NNA-induced hypertensive rats.

    PubMed

    Turgut Co?an, Didem; Saydam, Faruk; Özbayer, Cansu; Do?aner, Fulya; Soyocak, Ahu; Güne?, Hasan Veysi; De?irmenci, ?rfan; Kurt, Hülyam; Üstüner, Mehmet Cengiz; Bal, Cengiz

    2015-01-01

    Hypertension is a major health problem with increasing prevalence around the world. Tannic acid is water-soluble polyphenol that is present in tea, green tea, coffee, red wine, nuts, fruits and many plant foods. It has been reported to serve as an antioxidant or a pro-oxidant depending on the type of cells and its concentration. The purpose of our study was to evaluate the effect of tannic acid on systolic blood pressure, oxidative stress and some urinary parameters in the rat model of essential hypertension. Blood pressures of all rats were measured using the tail-cuff method. The nitric oxide synthase inhibitor N (omega)-nitro-L-arginine was administered orally at a dose of 0.5 g/l/day for 15 days to rats in order to create an animal model of hypertension. Tannic acid was intraperitoneally injected at a dose of 50 mg/kg for 15 days. Superoxide dismutase, catalase activity and the concentration of malondialdehyde (MDA) were determined in blood plasma and homogenates of heart, liver and kidney. In order to evaluate renal functions, urine pH, urine volume, urine creatine, uric acid, and urea nitrogen values were measured. Compared with the hypertension group, a decrease in MDA concentrations of heart tissue (p < 0.01), urea nitrogen values (p < 0.01) and urine volumes (p < 0.001) were established in hypertension + tannic acid group. There was also a decrease in blood pressure values (20th and 30th days) of this group, but there was no a statistical difference according to hypertension group. The findings of our research show the effect of tannic acid in lowering blood pressure in hypertensive rats. PMID:24306272

  15. The association of a simple blood pressure-independent parameter derived from ambulatory blood pressure variability with short-term mortality

    Microsoft Academic Search

    Benjamin Gavish; Iddo Z Ben-Dov; Jeremy D Kark; Judith Mekler; Michael Bursztyn

    2009-01-01

    We explored the predictive ability of the blood pressure variability ratio (BPVR), defined as the ratio of 24-h ambulatory systolic blood pressure variability to diastolic variability, and evaluated its predictable relation with blood pressure and the Ambulatory Arterial Stiffness Index (AASI). A total of 3433 consecutive patients were followed up to 16 years for all-cause mortality. Blood pressure variability was

  16. The effect of leaf-level spatial variability in photosynthetic capacity on biochemical parameter estimates using the Farquhar model: a theoretical analysis.

    PubMed

    Chen, Charles P; Zhu, Xin-Guang; Long, Stephen P

    2008-10-01

    Application of the widely used Farquhar model of photosynthesis in interpretation of gas exchange data assumes that photosynthetic properties are homogeneous throughout the leaf. Previous studies showed that heterogeneity in stomatal conductance (g(s)) across a leaf could affect the shape of the measured leaf photosynthetic CO(2) uptake rate (A) versus intercellular CO(2) concentration (C(i)) response curve and, in turn, estimation of the critical biochemical parameters of this model. These are the maximum rates of carboxylation (V(c,max)), whole-chain electron transport (J(max)), and triose-P utilization (V(TPU)). The effects of spatial variation in V(c,max,) J(max), and V(TPU) on estimation of leaf averages of these parameters from A-C(i) curves measured on a whole leaf have not been investigated. A mathematical model incorporating defined degrees of spatial variability in V(c,max) and J(max) was constructed. One hundred and ten theoretical leaves were simulated, each with the same average V(c,max) and J(max), but different coefficients of variation of the mean (CV(VJ)) and varying correlation between V(c,max) and J(max) (Omega). Additionally, the interaction of variation in V(c,max) and J(max) with heterogeneity in V(TPU), g(s), and light gradients within the leaf was also investigated. Transition from V(c,max)- to J(max)-limited photosynthesis in the A-C(i) curve was smooth in the most heterogeneous leaves, in contrast to a distinct inflection in the absence of heterogeneity. Spatial variability had little effect on the accuracy of estimation of V(c,max) and J(max) from A-C(i) curves when the two varied in concert (Omega = 1.0), but resulted in underestimation of both parameters when they varied independently (up to 12.5% in V(c,max) and 17.7% in J(max) at CV(VJ) = 50%; Omega = 0.3). Heterogeneity in V(TPU) also significantly affected parameter estimates, but effects of heterogeneity in g(s) or light gradients were comparatively small. If V(c,max) and J(max) derived from such heterogeneous leaves are used in models to project leaf photosynthesis, actual A is overestimated by up to 12% at the transition between V(c,max)- and J(max)-limited photosynthesis. This could have implications for both crop production and Earth system models, including projections of the effects of atmospheric change. PMID:18715955

  17. Nonlinear mathematical analysis of the hemodynamic parameters during left ventricular assistance with oscillated blood flow.

    PubMed

    Yambe, T; Kobayashi, S; Sonobe, T; Naganuma, S; Nanka, S; Hashimoto, H; Yoshizawa, M; Tabayashi, K; Takayasu, H; Takeda, H; Nitta, S

    1997-07-01

    For the development of a totally implantable ventricular assist system (VAS), we have been developing the vibrating flow pump (VFP), which can generate oscillated blood flow with a relatively high frequency (10-50 Hz) for a totally implantable system. In this study, effects of left ventricular assistance with this unique oscillated blood flow were analyzed by nonlinear mathematics for evaluation as the entire circulatory regulatory system, not as a separate part of the system. Left heart bypasses using VFPs from the left atriums to the descending aortas were performed in chronic animal experiments using healthy adult goats. Electrocardiogram (ECG), arterial blood pressure, VFP pump flow, and flow of the descending aorta data taken while the goats were awake were recorded in the data recorder and analyzed in the personal computer system through the AD convertor. Using nonlinear mathematics, time series data were embedded into the phase space, and the Lyapunov numerical method, fractal dimension analysis, and power spectrum analysis were performed to evaluate the nonlinear dynamics. During left ventricular assistance with the VFP, Mayer wave fluctuations were decreased in the power spectrum, the fractal dimension of the hemodynamics was significantly decreased, and peripheral vascular resistance was significantly decreased. These results suggest that nonlinear dynamics, which mediate the cardiovascular dynamics, may be affected during LV bypass with oscillated flow. Decreased power of the Mayer wave in the spectrum caused the limit cycle attractor of the hemodynamics and decreased the peripheral resistance. Decreased sympathetic discharges may be the origin of the decreased Mayer wave and fractal dimension. These nonlinear dynamical analyses may be useful to design the optimal VAS control. PMID:9212928

  18. Evaluation of low level laser therapy irradiation parameters on rat muscle inflammation through systemic blood cytokines

    NASA Astrophysics Data System (ADS)

    Mantineo, Matias; Pinheiro, João. P.; Morgado, António M.

    2014-02-01

    Low level laser therapy (LLLT) has been used for inflammation treatment. Here, we evaluate the effect of different doses, using continuous (830 and 980 nm) and pulsed illumination (830 nm), in the treatment of inflammation induced in the gastrocnemius muscle of Wistar rats, through cytokines concentration in systemic blood and histological analysis of muscle tissue. Animals were randomly divided into five groups per wavelength (5 animals per group: 10, 20, 30, 40 and 50 mW) plus a control group. LLLT was applied during five days, with constant exposure time and irradiated area (3 minutes; 0.5026 cm2). Blood was collected on days 0, 3 and 6. TNF-?, IL-1?, IL-2 and IL-6 cytokines were quantified by ELISA. Rats were killed on day 6. Muscle inflammatory cells were counted using optical microscopy. Treatment effects occurred for all applied doses (largest effect at 40 mW: 7.2 J, 14 J/cm2 per irradiation), with reduction of proinflammatory TNF-?, IL-1? and IL-6 cytokines and lower number of inflammatory cells. Results were better for 830 nm. Identical methodology was used with pulsed illumination. Average power (40 mW) and duty cycle were kept constant (80%) at five frequencies (5, 25, 50, 100 and 200 Hz). Treatment effects were observed at higher frequencies, with no significant differences between them. However, the treatment effect was lower than for continuous illumination. LLLT effect on inflammation treatment can be monitored by measuring systemic blood cytokines. A larger treatment effect was observed with continuous illumination, where results seem to be compatible with a biphasic dose response.

  19. The distribution patterns of trace elements in the blood and organs in a rabbit experimental model of copper pollution and study of haematology and biochemistry parameters

    Microsoft Academic Search

    Shi-Gang Shen; Hui Li; Yan-Yan Zhao; Qiu-Yan Zhang; Han-Wen Sun

    2005-01-01

    In a rabbit model of five copper overfeeding, we investigated the distribution pattern of trace elements of copper [Cu], zinc [Zn], chromium [Cr], manganese [Mn], and selenium [Se] in blood serum, red cell, and whole blood and in the organs: brain, gallbladder, liver, intestines, heart, kidney, lung, and spleen. Furthermore, their haematology and biochemistry parameters as well as feed consumption

  20. Estimation of genetic parameters for resistance to gastro-intestinal nematodes in pure blood Arabian horses.

    PubMed

    Korna?, S?awomir; Sallé, Guillaume; Skalska, Marta; David, Ingrid; Ricard, Anne; Cabaret, Jacques

    2015-03-01

    Equine internal parasites, mostly cyathostomins, affect both horse welfare and performance. The appearance of anthelmintic-resistant parasites creates a pressing need for optimising drenching schemes. This optimization may be achieved by identifying genetic markers associated with host susceptibility to infection and then to drench carriers of these markers. The aim of our study was to characterise the genetics of horse resistance to strongyle infection by estimating heritability of this trait in an Arabian pure blood population. A population of 789 Arabian pure blood horses from the Micha?ów stud farm, Poland were measured for strongyle egg excretion twice a year, over 8 years. Low repeatability values were found for faecal egg counts. Our analyses showed that less than 10% of the observed variation for strongyle faecal egg counts in this population had a genetic origin. However, additional analyses highlighted an age-dependent increase in heritability which was 0.04 (±0.02) in young horses (up to 3 years of age) but 0.21 (±0.04) in older ones. These results suggest that a significant part of the inter-individual variation has a genetic origin. This paves the way to a genomic dissection of horse-nematode interactions which might provide predictive markers of susceptibility, allowing individualised drenching schemes. PMID:25592965

  1. Hematological and Biochemical Reference Values for the Endangered Kiso Horse

    PubMed Central

    TAKASU, Masaki; NAGATANI, Nana; TOZAKI, Teruaki; KAKOI, Hironaga; MAEDA, Masami; MURASE, Tetsuma; MUKOYAMA, Harutaka

    2013-01-01

    To establish blood and biochemical references for the endangered Kiso horse, blood samples were collected from 111 adult Kiso horses, 74.5% of the existing breed. The samples were analyzed for 23 hematological and biochemical parameters to determine their means and standard deviations (SD). We compared the mean ± 2SD with the reference values cited in one of the most commonly used veterinary textbooks in Japan. The hematology of Kiso horses is characterized by lower erythrocyte count and hematocrit and hemoglobin levels. In addition, their serum biochemistry showed lower levels of aspartate transaminase, alkaline phosphatase, and ?-glutamyl transferase. Whether these propensities are attributed to breed-specific factors or are acquired factors remains unclear. Nevertheless, this study provides useful diagnostic indices for the endangered Kiso horse. PMID:24834006

  2. Effects of supplemental yeast (Saccharomyces cerevisiae) culture on rumen development, growth characteristics, and blood parameters in neonatal dairy calves.

    PubMed

    Lesmeister, K E; Heinrichs, A J; Gabler, M T

    2004-06-01

    Yeast (Saccharomyces cerevisiae) culture was added to a texturized calf starter at 0 (control), 1, or 2% of dry matter to determine effects on intake, growth, blood parameters, and rumen development. Seventy-five Holstein calves (38 male; 37 female) were started on the experiment at 2 +/- 1 d of age and were studied for 42 d. Starter intake was measured, and fecal scoring was conducted daily. Growth and blood parameter measurements were recorded at weekly intervals. A subset of 6 male calves (2 per treatment) was euthanized at 5 wk of age, and rumen tissue was sampled for rumen epithelial growth measurements. An additional 6 male calves were euthanized at 6 wk of age for rumen epithelial growth measurements. Inclusion of yeast culture at 2% of the starter ration significantly increased starter and total dry matter intake, average daily gain, and daily hip width change when compared with the control treatment. Average daily gain was improved by 15.6% for the 2% yeast treatment. Daily change in hip height was also significantly greater for calves receiving 2% supplemental yeast culture than for calves receiving 1%. No significant treatment differences were observed for any other variables. These data suggest that the addition of yeast culture in a dairy calf starter at 2% enhances dry matter intake and growth and slightly improves rumen development in dairy calves. PMID:15453499

  3. Blind Deconvolution for Distributed Parameter Systems with Unbounded Input and Output and Determining Blood Alcohol Concentration from Transdermal Biosensor Data.

    PubMed

    Rosen, I G; Luczak, Susan E; Weiss, Jordan

    2014-03-15

    We develop a blind deconvolution scheme for input-output systems described by distributed parameter systems with boundary input and output. An abstract functional analytic theory based on results for the linear quadratic control of infinite dimensional systems with unbounded input and output operators is presented. The blind deconvolution problem is then reformulated as a series of constrained linear and nonlinear optimization problems involving infinite dimensional dynamical systems. A finite dimensional approximation and convergence theory is developed. The theory is applied to the problem of estimating blood or breath alcohol concentration (respectively, BAC or BrAC) from biosensor-measured transdermal alcohol concentration (TAC) in the field. A distributed parameter model with boundary input and output is proposed for the transdermal transport of ethanol from the blood through the skin to the sensor. The problem of estimating BAC or BrAC from the TAC data is formulated as a blind deconvolution problem. A scheme to identify distinct drinking episodes in TAC data based on a Hodrick Prescott filter is discussed. Numerical results involving actual patient data are presented. PMID:24707065

  4. Numerical investigation of the hydrodynamic parameters of blood flow through stenotic descending aorta.

    PubMed

    Pasha Zanous, Sina; Shafaghat, Rouzbeh; Esmaili, Qadir

    2015-07-01

    In this study, the blood flow passing through a three-dimensional geometrically realistic stenosis is investigated both experimentally and numerically. Although the blood flow in stenotic arteries has been extensively studied in the past few decades, not much work has been focused on irregularity of stenosis. Thus, a model of an irregular stenotic descending aorta is used in this work. Due to the irregularity of stenosis model, the governing differential equations for continuity and momentum are solved numerically using finite-volume/finite-difference techniques in the generalized body-fitted coordinates. In order to verify the numerical results, the experimental measured pressure drops are compared with the numerical result. In addition, an improved method for nearly orthogonal grid generation is presented in numerical study. The grid generating system is based on the solution of a set of partial differential equations with finite difference discretization. Numerical calculations are performed to examine the effect of 55% (ratio between cross-sectional area at upstream and stenosis) irregular stenosis on the hemodynamic characteristics such as flow separation zone, wall shear stress and pressure drop. The maximum calculated wall shear stress is related to the maximum velocity gradient due to minimum cross-sectional area at the neck of stenosis. In addition, the pressure is shown as an important characteristic that is effecting on the resistance against the flow in the artery. Based on our results, the 55% irregular constriction is considered critical unlike the studies that have believed the reduction, which is greater than 75% become significant. PMID:26008888

  5. Arterial blood gas and spirometry parameters affect the length of stay in hospitalized asthmatic patients

    PubMed Central

    Mousavi, Seyed Ali Javad; Fereshtehnejad, Seyed-Mohammad; Khalili, Neda; Naghavi, Malihe; Yahyazadeh, Hooman

    2014-01-01

    Background: Asthma is a common condition in general medical practice, and it accounts for about 1% of all ambulatory hospital visits. Nowadays, hospitalization rates for asthma have actually been increased in some demographic subgroups despite recent advances in treatment. Understanding the underlying factors that contribute to hospitalization and especially duration of the hospitalization of asthmatics could help elucidate the recent rise in morbidity and also reduce the high demand on health care systems of the disease. The aim of this study was to evaluate factors affecting the duration of hospitalization for Iranian patients with asthma. Methods: This study was conducted on 55 asthmatic patients (diagnosis of asthma was in accordance with the criteria of the American Thoracic Society). The study was performed on patients hospitalized in Rasoul-e-Akram hospital in Tehran, Iran during the period 2005-2006. During hospitalization, the patients’ most common complaints were recorded as the symptoms and signs of the medical condition, results of physical examinations, spirometry, arterial blood gas analysis (ABG), and ICU admission. Results: There were 18(32.7%) male and 37(67.3%) female patients with a mean age of 54.96 (SD=17.54) years. The mean duration of hospitalization was 8.31(SD=4.69) days that ranged between 2 and 23 days. The mean baseline arterial PH (p=0.039, RPearso = -0.362), baseline arterial [HCO3] (p=0.042, RPearson = 0.361), changes of FEV1 after bronchodilator (p=0.041, RPearson= -0.363) and patient's age (p=0.002, RPearson=0.0433) were determined as factors affecting duration of hospitalization. Conclusion: Our results showed that more attention needs to be given to the findings of arterial blood gas and spirometry which can potentially affect the duration of hospitalization of asthmatic patients. PMID:25250249

  6. Effect of environmental parameters on lysosomal marker enzymes in the tropical blood clam Anadara granosa

    Microsoft Academic Search

    S. Patel; B. Patel

    1985-01-01

    The lysosomal marker enzymes, arylsulfatase and acid phosphatase, in a tropical burrowing arcid clam Anadara granosa L. have been found to exhibit seasonal variations. The activity of both enzymes decreased with increase in ambient temperature and fell with increase in salinity. Lysosomal latency for these enzymes, however, was not significantly affected by environmental parameters, including salinity, temperature, nutritional status, breeding

  7. The effect of Trifolium, Raphanus, and Cistus pollen grains on some blood parameters and mesentery mast cells.

    PubMed

    Kolankaya, Dürdane; Sentürk, Hakan; Tüylü, Asli Ozkök; Hayretda?, Sibel; Selmano?lu, Güldeniz; Sorkun, Kadriye

    2006-01-01

    Three kinds of pollen taxa belonging to 3 families (Fabaceae--Trifolium spp., Brassicaceae--Raphanus spp. and Cistaceae--Cistus spp.) and commonly collected by honeybees were fed to mature male rats separately, in the form of 60 mg/animal/day for a 30-day period. The objective of this study was to investigate any positive effects or possible side effects of the use of pollen on the immune system. This was achieved through blood analysis and cell count on blood, hemoglobin, erythrocyte and immune system cells. The cell concentration of mast cells, degranulization and cell localization were investigated in prepared mesentery tissue samples. Histological investigations of the stomach and duedenum sections of pollen-fed rats were carried out to learn the reason for eosinophil gastroenteritis in the alimentary canal. The eosinophil and lymphocyte levels of rats fed with pollen of Trifolium spp., Raphanus spp., and Cistus spp. were observed to have increased blood cell counts, while neutrophil and monocyte levels decreased; different values were found in basophil leucocytes between the pollen groups. Differing reductions in mesentery mast cell concentration, degranulization and cell localization were found. Within the three separate pollens, the rats having been fed with Cistus spp. pollen were observed to have higher blood lymphocyte, eosinophil, hemoglobin and hematocrit values than those fed with the others, as well as low mesentery mast cell concentration. Hemoglobin values were determined to increase at a proportion of between 10.0-11.3%. No difference was found in other blood parameters. The fat proportion of the male rats fed with the three taxa was between 4.03-8.75%, while that for protein proportion was between 16.11-24.25%. Male rats receiving these taxa did not experience allergic reactions and it is possible to argue that the low protein and fat content of these pollens have a strengthening effect on the immune systems by the increase in lymphocyte content and the amount of hemoglobin leads to an increase of oxygen transport capacity in the tissues. PMID:16869502

  8. Milk production, intake, digestion, blood parameters, and ingestive behavior of cows supplemented with by-products from the biodiesel industry.

    PubMed

    Gonzaga Neto, Severino; Oliveira, Ronaldo Lopes; de Lima, Francisco Helton Sá; de Medeiros, Ariosvaldo Nunes; Bezerra, Leilson Rocha; Viégas, Júlio; do Nascimento, Nilton Guedes; de Freitas Neto, Marcondes Dias

    2015-01-01

    This study aimed to evaluate the intake, digestion, blood parameters, and feeding behavior of crossbred dairy cows (Holstein?×?Gir) managed on Panicum maximum Jacq. cv. Tanzania-1 and provided supplementation with groundnut cake, sunflower cake, or palm kernel cake (to replace soybean meal). Sixteen cows were randomly assigned in a Latin square design with four treatments and four experimental periods. The consumption of nutrients from the pasture did not vary between experimental treatments. Cows receiving the palm kernel cake supplement had a reduced crude protein, non-fibrous carbohydrate, and total digestible nutrient intake and an increase in the average consumption of ether extract. There was also a reduction in the digestibility of dry matter. The inclusion of supplements in the diet did not influence the average time spent eating, ruminating, or resting. The mean values of respiratory and heart rates showed thermal comfort during the trial period. There was a reduction in blood urea nitrogen with palm kernel cake supplementation, and the values of total protein, albumin, and glucose were also significantly different with this supplement. It is recommended that cakes of groundnut cake and sunflower cake seed be used for a total replacement of soybean meal supplements for lactating cows, but the use of palm kernel cake is not recommended. PMID:25319453

  9. Effect of pesticide residues on health and blood parameters of farm workers from rural Gadap, Karachi, Pakistan.

    PubMed

    Azmi, M Ahmed; Naqvi, S N H; Akhtar, Kehkashan; Moinuddin; Parveen, Shahida; Parveen, Rehana; Aslam, M

    2009-09-01

    The samples were collected from 83 persons on 1 day (reading-I), 7 days (reading-II) and 30 days (reading-III) engaged in pesticide spraying in fourteen different fruit and vegetable farm stations located in the orchards of Gadap (rural area), Karachi-Pakistan. In the present study 26 workers out of 83 who were exposed to different pesticides (ie., cypermethrin, deltamethrin, polytrin-C, diazninon, monocrotophos, DDT and DDE) were compared with 25 healthy control persons. Different blood parameters were determined in comparison to control subjects. The results indicate some alterations in blood indices in allthe pesticide exposed persons, and only two persons from two different stations were affected severely and therefore their Hb, MCV MCHC, TLC, monocyte and neutrophil counts increased significantly. High lymphocyte count was noted in almost all the exposed persons. Platelets count was also found high in few persons but Hb, MCV, MCH, TLC, RBC and neutrophil counts significantly decreased in such persons. Exposure of multiple pesticides for prolong period has also affected the health of exposed persons and produced dermatological, hepatic, nephritic, respiratory and other clinical disorders reflecting the toxic effects of pesticides. Our findings indicate that indiscriminate use of pesticides in farming environments must be regularly assessed and farm workers must be trained for safe use of pesticides. PMID:20136060

  10. Correlation of ultrasound contrast agent derived blood flow parameters with immunohistochemical angiogenesis markers in murine xenograft tumor models

    PubMed Central

    Eisenbrey, John R.; Wilson, Christian C.; Ro, Raymond J.; Fox, Traci B; Liu, Ji-Bin; Chiou, See-Ying; Forsberg, Flemming

    2013-01-01

    Purpose In this study we used temporal analysis of ultrasound contrast agent (UCA) wash-in to estimate blood flow dynamics and demonstrate their improved correlation to angiogenesis markers relative to previously reported, non-temporal fractional vascularity estimates. Materials and Methods Breast tumor (NMU) or glioma (C6) cells were implanted in either the abdomen or thigh of 144 rats. After 6, 8 or 10 days, rats received a bolus UCA injection of Optison (GE Healthcare, Princeton, NJ; 0.4ml/kg) during power Doppler imaging (PDI), harmonic imaging (HI), and microflow imaging (MFI) using an Aplio ultrasound scanner with 7.5 MHz linear array (Toshiba America Medical Systems, Tustin, CA). Time-intensity curves of contrast wash-in were constructed on a pixel-by-pixel basis and averaged to calculate maximum intensity, time to peak, perfusion, and time integrated intensity (TII). Tumors were then stained for 4 immunohistochemical markers (bFGF, CD31, COX-2, and VEGF). Correlations between temporal parameters and the angiogenesis markers were investigated for each imaging mode. Effects of tumor model and implant location on these correlations were also investigated. Results Significant correlation over the entire dataset was only observed between TII and VEGF for all three imaging modes (R=?0.35,?0.54,?0.32 for PDI, HI and MFI, respectively; p<0.0001). Tumor type and location affected these correlations, with the strongest correlation of TII to VEGF found to be with implanted C6 cells (R=?0.43,?0.54,?0.52 for PDI, HI and MFI, respectively; p<0.0002). Conclusions While UCA-derived temporal blood flow parameters were found to correlate strongly with VEGF expression, these correlations were also found to be influenced by both tumor type and implant location. PMID:23659876

  11. Rapid Analysis of Hemoglobins in Whole Blood by a Light Scattering Method

    NASA Astrophysics Data System (ADS)

    Lisenko, S. A.; Kugeiko, M. M.

    2013-07-01

    We have developed a method for fast determination of blood morphological and biochemical parameters (concentration of oxyhemoglobin, deoxyhemoglobin, methemoglobin, and sulfhemoglobin; the hematocrit; average concentration of total hemoglobin in erythrocytes; the erythrocyte parameter associated with their volume and shape) from the diffuse transmittance spectrum of light for a thin cuvet containing whole blood (thickness 200 ?m), illuminated by collimated light. The method is based on an analytical dependence of the spectral diffuse transmission coefficient of blood on the parameters of interest, taking into account multiple scattering of light by erythrocytes and possible variations in their shape. We have analyzed the uncertainties of the method when the analyte parameters are all variable.

  12. Effect of ?-hydroxy-?-methylbutyrate calcium on growth, blood parameters, and carcass qualities of broiler chickens.

    PubMed

    Qiao, X; Zhang, H J; Wu, S G; Yue, H Y; Zuo, J J; Feng, D Y; Qi, G H

    2013-03-01

    Beta-hydroxy-?-methylbutyrate (HMB), the metabolite of leucine, plays an important role in muscle protein metabolism. To investigate the effect of dietary HMB calcium (HMB-Ca) on growth performance, breast muscle development, and serum parameters in broiler chickens, a total of two hundred seventy 1-d-old Arbor Acres male broiler chicks were randomly allotted into 3 dietary treatments supplemented with 0, 0.05%, or 0.1% HMB-Ca during the starter (1 to 21 d) and grower (22 to 42 d) period. The results showed that broilers fed 0.1% HMB-Ca diet had higher ADG during the starter or the whole period, and gain 148 g more BW than the chicks fed the control diet at 42 d of age (P < 0.05). At 21 d of age, birds receiving 0.1% HMB-Ca had more breast muscle yield, less abdominal fat than the control, and more dressing percentage than birds fed the control or 0.05% HMB-Ca diet (P < 0.05). At 42 d of age, 0.1% HMB-Ca increased breast muscle yield than the control and decreased abdominal fat compared with the control or 0.05% HMB-Ca group (P < 0.05). In comparison with the control, feeding 0.1% HMB-Ca increased the triiodothyronine, thyroxine, triiodothyronine/thyroxine ratio and decreased the serum uric acid level at d 21 (P < 0.05). At 42 d of age, serum thyroxine level was elevated in the 0.05% HMB-Ca treatment, and the uric acid concentration was significantly decreased by the 0.1% HMB-Ca-supplemented diet (P < 0.05). Dietary HMB-Ca did not affect the growth hormone or insulin content. This study suggested that dietary supplementation of HMB-Ca improved growth performance, stimulated the breast muscle development, and decreased the abdominal fat deposition in broiler chickens, and the favorable effects were more pronounced in the starter phase. The growth promotion effect of HMB-Ca may be partly related to the increased serum thyroid hormones in broiler chickens. PMID:23436526

  13. Biochemical responses to fibropapilloma and captivity in the green turtle.

    PubMed

    Swimmer, J Y

    2000-01-01

    Blood biochemical parameters were compared for green turtles (Chelonia mydas) with and without green turtle fibropapillomatosis (GTFP) from both captive and wild populations in Hawaii (USA) and from a captive population from California (USA), during the period between 1994 and 1996. Statistical analysis did not detect an influence of disease in any of the blood parameters for free-ranging turtles; however, captive turtles in Hawaii with GTFP had significantly higher levels of alkaline phosphatase and significantly lower levels of lactate compared to non-tumored captive turtles. Multivariate analysis found that biochemical profiles could be used to accurately predict if turtles were healthy or afflicted with GTFP. Discriminant function analysis correctly classified turtles as being with or without GTFP in 89% of cases, suggesting that diseased animals had a distinct signature of plasma biochemistries. Measurements of blood parameters identified numerous differences between captive and wild green turtles in Hawaii. Levels of corticosterone, lactate, triglyceride, glucose, and calcium were significantly higher in wild green turtles as compared to captive turtles, while uric acid levels were significantly lower in wild turtles as compared to captive turtles. Additionally, turtles from Sea World of California (San Diego, California, USA), which had been in captivity the longest, had higher levels of alanine aminotransferase and triglycerides as compared to nearly all other groups. Differences in diet, sampling methods, environmental conditions, and turtle size, help to interpret these results. PMID:10682751

  14. Reducing the number of parameters in 1D arterial blood flow modeling: less is more for patient-specific simulations.

    PubMed

    Epstein, Sally; Willemet, Marie; Chowienczyk, Phil J; Alastruey, Jordi

    2015-07-01

    Patient-specific one-dimensional (1D) blood flow modeling requires estimating model parameters from available clinical data, ideally acquired noninvasively. The larger the number of arterial segments in a distributed 1D model, the greater the number of input parameters that need to be estimated. We investigated the effect of a reduction in the number of arterial segments in a given distributed 1D model on the shape of the simulated pressure and flow waveforms. This is achieved by systematically lumping peripheral 1D model branches into windkessel models that preserve the net resistance and total compliance of the original model. We applied our methodology to a model of the 55 larger systemic arteries in the human and to an extended 67-artery model that contains the digital arteries that perfuse the fingers. Results show good agreement in the shape of the aortic and digital waveforms between the original 55-artery (67-artery) and reduced 21-artery (37-artery) models. Reducing the number of segments also enables us to investigate the effect of arterial network topology (and hence reflection sites) on the shape of waveforms. Results show that wave reflections in the thoracic aorta and renal arteries play an important role in shaping the aortic pressure and flow waves and in generating the second peak of the digital pressure and flow waves. Our novel methodology is important to simplify the computational domain while maintaining the precision of the numerical predictions and to assess the effect of wave reflections. PMID:25888513

  15. Effects of physical activity on blood pressure monitoring and morphometric parameters of the left ventricle and of the abdominal aorta in healthy elderly subjects.

    PubMed

    Macchi, Claudio; Lova, Raffaele Molino; Giannelli, Fabrizio; Papucci, Mario; Conti, Andrea A; Paternostro, Ferdinando; Contini, Massimo; Miniati, Benedetta; Gulisano, Massimo; Catini, Claudio

    2003-01-01

    To investigate the effects of physical activity not only on morphometric left ventricular parameters, but also on the abdominal aorta diameter and on blood pressure, we enrolled 100 healthy subjects, 50 males and 50 females, aged between 63 and 91 years (mean 73.3 +/- s.d. 7.7), each divided into two subgroups, sedentary and non sedentary, come to our observation for a diagnostic screening. For each subject an Echography was performed, by using an Acuson 128XP10 apparatus, equipped with a 2 MHz phased array probe, both for the study of the heart and for the study of the abdominal aorta. Then, all the subjects underwent 24 hours blood pressure monitoring by using a P6 Delmar apparatus in order to get mean systolic and diastolic blood pressure. Diastolic blood pressure was significantly related to physical activity (sedentary > non sedentary, P < 0.01), while systolic blood pressure was not; left ventricle and abdominal aorta diameters were both significantly related to sex (males > females, P < 0.001), but left ventricle diameter was significantly related to diastolic blood pressure (P < 0.01), while abdominal aorta diameter was significantly related to systolic blood pressure (P < 0.001). Our data suggest that physical activity might be strongly recommended to control blood pressure. PMID:14974503

  16. Effects of modified electroconvulsive therapy on the cognitive function and blood parameters in female patients with schizophrenia

    PubMed Central

    Jiang, Yansheng; Zhang, Hongxing; Wang, Zifan; Zhao, Ling; Lv, Luxian

    2015-01-01

    Background: This study aimed to investigate the effects of modified electroconvulsive therapy (MECT) on cognitive function and blood parameters in female patients with schizophrenia. Materials and methods: Female patients with schizophrenia (n = 23) received MECT while maintaining antipsychotic therapy. 1) White blood cell (WBC), alanine aminotransferase (ALT), creatine kinase (CK) and creatine kinase MB (CKMB) were measured at 10 min before and after MECT. 2) The severity of symptoms was evaluated before and after MECT by using the Positive and Negative Symptoms Scale (PANSS) and then the therapeutic effects of MECT were assessed. 3) Single nerve psychology test was used to assess the cognitive function. Results: 1) There were no significant differences in WBC, ALT, CK and CKMB before and after MECT (P > 0.05). 2) WBC, ALT and CKMB remained stable at different time points after MECT treatment (P > 0.05). But CK had statistical differences at different times before or after MECT treatment (P < 0.05). CK decreased since the first MECT and thereafter increased after the 7th treatment (P < 0.05). 3) The total score of PANSS decreased significantly after MECT (P < 0.05). 4) Digit span test showed no statistically significant differences in different time points (P > 0.05); Digital sign test and verbal fluency test showed significant differences in different times (P < 0.05). Conclusion: The CK figure decreased from the first to sixth MECT treatment and increased in the 7th MECT treatment, and the CKMB also increased in the 7th treatment. MECT treatment had significant effects on female patients with schizophrenia and could obviously improve patient’s cognitive function. PMID:25785136

  17. The influence of the luteal and follicular phases on major pharmacokinetic parameters of blood and breath alcohol kinetics in women.

    PubMed

    Dettling, Andrea; Preiss, Astrid; Skopp, Gisela; Haffner, Hans-Thomas

    2010-06-01

    Drink tests involving 14 women were carried out to determine the effects of the menstrual cycle phases on the pharmacokinetics of ethanol. One experiment was carried out in the follicular phase of the cycle and another in the luteal phase, with the estradiol, progesterone, and testosterone levels being determined in both cases. The target concentration was a final blood alcohol concentration (BAC) of approximately 0.08g%. After drinking was completed, concurrent BAC and breath alcohol concentration (BrAC) measurements were carried out at intervals of 10-20min. The ethanol elimination rate was determined by calculating a linear function in the part of the slope that was clearly linear. In addition, the c(0) and Widmark factors r were calculated. In 10 of the volunteers, who had a normal increase in progesterone in the luteal phase, the average hourly elimination rate ss(60) in the follicular phase amounted to 0.0194+/-0.0020g%/h (BAC) and 0.0975+/-0.0068mg/L/h (BrAC), and in the luteal phase to 0.0193+/-0.0031g%/h (BAC) and 0.1026+/-0.0101mg/L/h (BrAC). There was no significant difference. Other pharmacokinetic parameters (c(0) concentrations, Widmark factors r, distribution volumes, maximal BAC, mean absorption rate, time until the peak concentrations were reached) also revealed no significant differences between the blood and breath alcohol levels of the luteal and follicular phases. In addition, no significant correlations were observed between the absolute progesterone level and the respective elimination rates ss(60). PMID:20570083

  18. Meat quality, oxidative stability and blood parameters from Graylag geese offered alternative fiber sources in growing period.

    PubMed

    He, L W; Meng, Q X; Li, D Y; Zhang, Y W; Ren, L P

    2015-04-01

    The effects of dietary fiber sources on the meat quality, oxidative stability, and blood parameters of growing Graylag geese (28-112d) were investigated. The birds were randomly allocated into 4 treatments, of which dietary fiber was mainly from corn straw silage ( CSS: ), steam-exploded corn straw ( SECS: ), steam-exploded wheat straw ( SEWS: ), and steam-exploded rice straw ( SERS: ). No influence (P > 0.05) on the basic chemical components, oxidative stability, or organoleptic traits of muscle were observed, except that birds fed SECS had a higher (P < 0.05) protein proportion than those fed CSS or SERS, and CSS increased (P < 0.01) the cholesterol content when compared to SEWS or SERS. Regarding fatty acid profile in meat, CSS and SECS increased (P < 0.01) the proportion of C18:2n6t and decreased that of C21:0 and C22:0 when compared to the others. The birds fed SERS had a higher (P < 0.05) proportion of C20:0 and C22:0 than the others, a higher proportion of C20:5n3, n-3 fatty acids, ?-9 desaturase (18) index compared to those fed CSS or SECS, and a lower (P < 0.01) proportion of C20:1n9 than those fed SECS or SEWS. Additionally, SEWS resulted in a higher (P < 0.01) proportion of C20:2 when compared to the others. In conclusion, these fibers affect just the protein proportion, cholesterol content, and fatty acid profile of breast muscle, along with the concentration of TG and MDA in blood, but not the other characteristics. No superior fiber source exists with respect to meat quality, suggesting that Graylag geese feeding should make the most economically of the convenient fiber source with appropriate pretreatment. PMID:25717090

  19. Variability of biochemical parameters and radiation resistance of the seed progeny of Descurainia sophia and Lepidium apetalum under exposure to various factors

    Microsoft Academic Search

    I. A. Prokop’ev; A. N. Zhuravskaya; G. V. Filippova

    2011-01-01

    The physiological and biochemical characteristics of seedlings of Descurainia sophia (Fisch.) and Lepidium apetalum Wild. have been used as examples to demonstrate that long-term exposure of plant populations to environmental stress factors\\u000a causes adaptive changes in antioxidant and genomic systems that may considerably affect the radioresistance of seed progeny.\\u000a The resistance to additional acute irradiation of seed progeny of wild

  20. BLOOD PROFILES FOR A WILD POPULATION OF GREEN TURTLES (CHELONIA MYDAS) IN THE SOUTHERN BAHAMAS: SIZE-SPECIFIC AND SEX-SPECIFIC RELATIONSHIPS

    Microsoft Academic Search

    Alan B. Bolten; Karen A. Bjomdal

    1992-01-01

    Blood biochemical profiles and packed cell volumes were determined for 100 juvenile green turtles, Chelonia mydas, from a wild population in the southern Bahamas. There was a significant correlation of body size to 13 of the 26 blood parameters measured. Only plasma uric acid and cholesterol were significantly different between male and female turtles. The relationship between total plasma proteins

  1. a statistical model based on serological parameters for predicting occult HBV infection: implications for organ/ blood donations.

    PubMed

    Coen, Sabrina; Angeletti, Claudio; Piselli, Pierluca; Tronchin, Michele; Vincenti, Donatella; Capobianchi, Maria Rosaria; Galli, Claudio; Menzo, Stefano

    2015-01-01

    The transmission of hepatitis B virus by donors with occult HBV infection (OBI) is a threat for blood transfusion and organ/tissue transplantation. The risk of carrying HBV DNA is currently not predictable by simple serologic markers, while HBV DNA testing is not universally deployed. This study evaluated an integrated serologic approach for assessing this risk. Anti-HBc positive subjects (461 HIV-negative, 262 HIV-positive) were selected for the study. Serology was analyzed by a commercial CMIA technique. HBV DNA was analyzed by both commercial and home-brew real-time amplification assays. A penalized maximum likelihood logistic approach was used to analyze the data. In HBsAg-negative subjects (HIV-negative), anti-HBc signal/cut off values, the presence of anti-HBc IgM, the absence of anti-HBsAg, and the absence of anti-HCV were correlated to the probability of finding circulating HBV DNA. A model for predicting HBV DNA presence by 4 serological parameters is therefore proposed. The predictive value of the logistic model based on simple serologic markers may represent a reasonable tool for the assessment of HBV transmission risk by transfusion or organ/tissue donation in the context of limited resources and where nucleic acid testing is not performed. In addition, it may be helpful for assessing the risk of reactivation in immunosuppressed OBI patients. PMID:25742146

  2. The Effects of Accelerated Growth Rates and Estrogen Implants in Prepubertal Holstein Heifers on Growth, Feed Efficiency, and Blood Parameters1

    Microsoft Academic Search

    B. P. Lammers; A. J. Heinrichs; R. S. Kensinger

    1999-01-01

    Sixty-eight Holstein heifers were used to determine the effects of accelerated growth rates by increased nutrient intake and estrogen implants on feed effi- ciency, structural growth, and blood parameters in heifers between 19 and 39 wk of age. At the beginning of the treatment period, the heifers were assigned to one of four treatment groups by using a randomized complete

  3. Effects of Adding Extra Molasses to a Texturized Calf Starter on Rumen Development, Growth Characteristics, and Blood Parameters in Neonatal Dairy Calves

    Microsoft Academic Search

    K. E. Lesmeister; A. J. Heinrichs

    2005-01-01

    A texturized calf starter containing 5 (control) or 12% molasses (on a dry matter (DM) basis) was fed to dairy calves to determine effects on intake, growth, blood parameters, and rumen development. Forty-six Hol- stein calves (26 male and 20 female) were started at 2 ± 1 d of age and studied for 42 d. Starter DM intake was measured

  4. Weight gains, blood parameters, and fecal egg counts when meat-goat kids were finished on alfalfa, red clover, or orchardgrass pastures

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This experiment was conducted in 2005-2007 to evaluate weight gain, blood parameters associated with forage nutrient-use and anemia from gastrointestinal (GI) parasite infection, and fecal egg counts (FEC) patterns of meat goat kids finished on alfalfa (Medicago sativa L; ALF); red clover (Trifolium...

  5. Effects of rumen-protected choline and dry propylene glycol on feed intake and blood parameters for Holstein dairy cows in early lactation

    Microsoft Academic Search

    Y.-H. Chung; N. E. Brown; C. M. Martinez; T. W. Cassidy; G. A. Varga

    2009-01-01

    A 6 × 6 Latin square design was used to test 3 sets of comparisons simultaneously to study response in dry matter intake, milk yield, and blood parameters to propylene glycol (PG) supplementation delivered by 2 methods (incorporating PG into the total mixed ration (TMR) vs. top dressing; comparison I); individual or combined dietary choline and PG supplementation as a

  6. [The changes in the biochemical indices of blood in cobalt intoxication on the background of the regulators of the expression of endothelial NO-synthase].

    PubMed

    Dzugkoev, S G; Mozhayeva, I V; Gigolaeva, L B; Tedtoeva, A I; Margieva, O I; Dzugkoeva, F S

    2014-01-01

    On the background of chronic cobalt intoxication in rats develops oxidative stress. This is accompanied by a decrease in the content of total metabolites of nitric oxide in the blood serum. The reasons for lower levels of the vasodilatation factor are the following: a deficiency of L-arginine and the increase in the content of the inhibitor of NOS-3. We found a decrease in the bioavailability of nitric oxide due to the increase in the content of oxidized low density lipids, total cholesterol and cholesterol-lowering lipids high density. PMID:25980229

  7. Effect of graded physical load on the state of the liver from morphometric data and biochemical blood indices of rats against a background of hypokinesia

    NASA Technical Reports Server (NTRS)

    Nikityuk, B. A.; Kogan, B. I.; Yermolyev, V. A.; Tindare, L. V.

    1980-01-01

    Tests were conducted on 100 sexually immature inbred August and Wistar male rats in order to determine the effects hypokinesia, physical load and phenamine on the liver. Weight and linear dimension fell in hypokinesia; total serum protein lowered and aldolase and cholesterol and beta-lipoprotein levels rose. Blood sugar content rose and liver glycogen fell. Interlinear differences of these indices are found. Rehabilitated physical loading against hypokinesia background diminished and at times completely prevented its negative effect. Extent of correction depended on animal species. Evidence of genotypical conditionality of organism adaptation to physical load in hypokinesia was found.

  8. Unification of some biochemical methods of research in the pre- and post-flight periods

    NASA Technical Reports Server (NTRS)

    Tigranyan, R. A.

    1980-01-01

    The biochemical methods for determination of various parameters and factors during pre- and post-flight periods, as used by American and Soviet teams dealing with space flight medicine are compared. The emphasis is on the exchange of information on the study of the blood and urine content of space travelers before and after space flight. A series of electrolytic, enzymatic, and hormonal factors is discussed.

  9. Sensitivity of compliance-based continuous non-invasive blood pressure monitoring to changes in viscoelastic parameters

    Microsoft Academic Search

    A. M. Dentinger; C. Jia

    2011-01-01

    The apparent arterial compliance observed from non- invasive measurements of arterial blood pressure and cross- sectional area does not directly correspond to the static compliance curve or dynamic pressure-area loop. The accuracy of continuous, non-invasive blood pressure estimates based on calibrated arterial compliance models to convert area to pressure is affected by both changes in the viscoelastic properties of the

  10. Selected chemical parameters in the blood and metals in the organs of the Nile crocodile, Crocodylus Niloticus, in the Kruger National Park.

    PubMed

    Swanepoel, D; Boomker, J; Kriek, N P

    2000-06-01

    Healthy and sick crocodiles of varying sizes were examined from the Olifants River in the central part of the Kruger National Park, the Sabi River in the southern part and the Shingwedzi River in the northern region. Blood was collected for the determination of certain parameters and samples of fat, muscle, kidney and liver tissue were collected and analyzed for their heavy metal content. The results of the blood analyses are within the range recorded in the literature, but the metal analyses were inconclusive as similar data are not available for comparison. The results of the metal analyses are presented here for use as baseline and reference data. PMID:11028751

  11. Dietary Echium Oil Increases Long-Chain n–3 PUFAs, Including Docosapentaenoic Acid, in Blood Fractions and Alters Biochemical Markers for Cardiovascular Disease Independently of Age, Sex, and Metabolic Syndrome12

    PubMed Central

    Kuhnt, Katrin; Fuhrmann, Claudia; Köhler, Melanie; Kiehntopf, Michael; Jahreis, Gerhard

    2014-01-01

    Dietary supplementation with echium oil (EO) containing stearidonic acid (SDA) is a plant-based strategy to improve long-chain (LC) n–3 (?-3) polyunsaturated fatty acid (PUFA) status in humans. We investigated the effect of EO on LC n–3 PUFA accumulation in blood and biochemical markers with respect to age, sex, and metabolic syndrome. This double-blind, parallel-arm, randomized controlled study started with a 2-wk run-in period, during which participants (n = 80) were administered 17 g/d run-in oil. Normal-weight individuals from 2 age groups (20–35 and 49–69 y) were allotted to EO or fish oil (FO; control) groups. During the 8-wk intervention, participants were administered either 17 g/d EO (2 g SDA; n = 59) or FO [1.9 g eicosapentaenoic acid (EPA); n = 19]. Overweight individuals with metabolic syndrome (n = 19) were recruited for EO treatment only. During the 10-wk study, the participants followed a dietary n–3 PUFA restriction, e.g., no fish. After the 8-wk EO treatment, increases in the LC n–3 metabolites EPA (168% and 79%) and docosapentaenoic acid [DPA (68% and 39%)] were observed, whereas docosahexaenoic acid (DHA) decreased (?5% and ?23%) in plasma and peripheral blood mononuclear cells, respectively. Compared with FO, the efficacy of EO to increase EPA and DPA in blood was significantly lower (?25% and ?50%, respectively). A higher body mass index (BMI) was associated with lower relative and net increases in EPA and DPA. Compared with baseline, EO significantly reduced serum cholesterol, LDL cholesterol, oxidized LDL, and triglyceride (TG), but also HDL cholesterol, regardless of age and BMI. In the FO group, only TG decreased. Overall, daily intake of 15–20 g EO increased EPA and DPA in blood but had no influence on DHA. EO lowered cardiovascular risk markers, e.g., serum TG, which is particularly relevant for individuals with metabolic syndrome. Natural EO could be a noteworthy source of n–3 PUFA in human nutrition. This trial was registered at clinicaltrials.gov as NCT01856179. PMID:24553695

  12. [Effect of excretion-secretion products of some fouling species on the biochemical parameters of blue mussel Mytilus edulis L. (Mollusca: Bivalvia) in the White Sea].

    PubMed

    Skidchenko, V S; Vysotskaia, R U; Krupnova, M Iu; Khalaman, V V

    2011-01-01

    The effect of excretion-secretion products (ESP) of five abundant fouling invertebrate species (bivalve mollusks Hiatella arctica and Mytilus edulis, solitary ascidia Styela rustica, sponge Halichondria panicea, and sea starAsterias rubens, inhabiting the White Sea) on the biochemical status of blue mussel M. edulis was assessed by the dynamics of lysosomal enzymes activity (nucleases, glycoside hydrolases, and cathepsins). ESP of conspecific species had no effect on the metabolism of the mollusks of this species. ESP of A. rubens, S. rustica, and H. panicea activated the same enzymes. First, acid RNase and glycoside hydrolases activity increased, but in different ways. The metabolites of H. arctica affected the activity of proteometabolism enzymes. PMID:22292287

  13. The effect of hormone therapy on biochemical and ultrasound parameters associated with atherosclerosis in 46,XY DSD individuals with female phenotype.

    PubMed

    Tsimaris, Pantelis; Deligeoroglou, Efthimios; Athanasopoulos, Nikolaos; Economou, Emmanuel; Stamatelopoulos, Kimon; Rizos, Demetrios; Papamichael, Christos; Lambrinoudaki, Irene; Mastorakos, George; Creatsas, George

    2014-10-01

    The aim of this study was to evaluate the effect of hormone therapy (HT) in the endothelial function of 46,XY disorders of sexual development (DSD) patients with female phenotype. Biochemical and ultrasound measurements were performed in 20 patients at initiation of oral 2?mg 17?-estradiol/1?mg norethisterone acetate, and after 6 months of therapy. Lipid profile, including total cholesterol (TC), LDL, HDL, triglycerides (TG) and Atherogenic Index of Plasma (AIP), as well as levels of VE-Cadherin, E-Selectin, Thrombomodulin and vWf were determined. Ultrasonographic examinations included evaluation of flow-mediated dilatation (FMD) and measurement of Carotid and Femoral Intima Media Thickness (IMT). HT raised HDL (35.4?mg/dl versus 40.1?mg/dl, p?=?0.019) while lowering TG (166?mg/dl versus 109?mg/dl, p?=?0.026) and AIP (0.24 versus 0.04, p?=?0.007). No changes were noted in TC and LDL (215.7?mg/dl versus 192.25?mg/dl and 87.46?mg/dl versus 76.35?mg/dl, respectively). There was significant reduction of VE-Cadherin (4.05?ng/ml versus 2.20?ng/ml, p?=?0.002) and E-selectin (73.98?ng/ml versus 56.73?ng/ml, p?=?0.004). No change was observed in Thrombomodulin and vWf (11.76?ng/ml versus 13.90?ng/ml and 80.75% versus 79.55%, respectively). FMD improved significantly (5.4% versus 8.15%, p?=?0.003), while only carotid bulb IMT decreased significantly (0.65?mm versus 0.60?mm, p?=?0.018). Overall, HT was found to improve biochemical and ultrasound markers of endothelial function in 46,XY DSD patients with female phenotype. PMID:24911331

  14. High Initial Blood Levels of Tacrolimus in Overweight Renal Transplant Recipients

    Microsoft Academic Search

    E. Rodrigo; M. A. de Cos; B. Sánchez; J. C. Ruiz; C. Piñera; G. Fernández-Fresnedo; R. Palomar; M. A. Pérez-Ceballos; J. G. Cotorruelo; J. A. Zubimendi; A. L. M. de Francisco; M. Arias

    2005-01-01

    For the purpose of both efficacy and safety, exposure to tacrolimus and other immunosuppressive drugs must be monitored, since initial levels influence the development of acute rejection episodes, nephrotoxicity, and posttransplantation diabetes mellitus. The aim of this study was to identify risk factors for developing high initial tacrolimus blood levels. We analyzed clinical and biochemical parameters of 85 renal transplant

  15. Whole Blood Transcriptomics and Urinary Metabolomics to Define Adaptive Biochemical Pathways of High-Intensity Exercise in 50-60 Year Old Masters Athletes

    PubMed Central

    Mukherjee, Kamalika; Edgett, Brittany A.; Burrows, Harrison W.; Castro, Cecilia; Griffin, Julian L.; Schwertani, Adel Giaid; Gurd, Brendon J.; Funk, Colin D.

    2014-01-01

    Exercise is beneficial for a variety of age-related disorders. However, the molecular mechanisms mediating the beneficial adaptations to exercise in older adults are not well understood. The aim of the current study was to utilize a dual approach to characterize the genetic and metabolic adaptive pathways altered by exercise in veteran athletes and age-matched untrained individuals. Two groups of 50–60 year old males: competitive cyclists (athletes, n?=?9; VO2peak 59.1±5.2 ml·kg?1·min?1; peak aerobic power 383±39 W) and untrained, minimally active individuals (controls, n?=?8; VO2peak 35.9±9.7 ml·kg?1·min?1; peak aerobic power 230±57 W) were examined. All participants completed an acute bout of submaximal endurance exercise, and blood and urine samples pre- and post-exercise were analyzed for gene expression and metabolic changes utilizing genome-wide DNA microarray analysis and NMR spectroscopy-based metabolomics, respectively. Our results indicate distinct differences in gene and metabolite expression involving energy metabolism, lipids, insulin signaling and cardiovascular function between the two groups. These findings may lead to new insights into beneficial signaling pathways of healthy aging and help identify surrogate markers for monitoring exercise and training load. PMID:24643011

  16. Blood feeding in juvenile Paragnathia formica (Isopoda: Gnathiidae): biochemical characterization of trypsin inhibitors, detection of anticoagulants, and molecular identification of fish hosts.

    PubMed

    Manship, B M; Walker, A J; Jones, L A; Davies, A J

    2012-05-01

    The 3 post-marsupial juvenile stages of the gnathiid isopod, Paragnathia formica, are haematophagous ectoparasites of fishes that may, in heavy infestations, cause host mortality. Protein digestion in fed stage 3 juveniles is accomplished by cysteine proteinases, but what bioactive compounds attenuate host haemostatic, inflammatory and immunological responses during feeding is unknown. Trypsin inhibitory activity and anticoagulant activity were detected in crude extracts of unfed P. formica stage 1 juveniles; fractionation of stage 1 crude extracts by ion exchange chromatography resulted in 3 preparations each displaying these bioactivities. Further characterization revealed anti-thrombin activity in 2 of these preparations, whilst the third displayed the strongest anticoagulant activity that targeted a factor of the intrinsic coagulation pathway. Three trypsin inhibitors (18 kDa, 21 kDa, and 22 kDa) were also detected using reverse zymography. In parallel, homogenates of fed stage 2 and 3 juveniles were used to identify their fish hosts by amplifying the 16S mitochondrial rDNA and 18S genomic rDNA vertebrate gene regions. Blood from at least 4 fish families had been ingested by separate individuals during feeding. This study demonstrates that trypsin inhibitors and anticoagulants are present in P. formica juveniles which could suppress host haemostatic, inflammatory and immunological responses during feeding, and that juveniles are not host specific. PMID:22309997

  17. Cadmium-induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and ?-carotene

    Microsoft Academic Search

    Fatma M El-Demerdash; Mokhtar I Yousef; Fatma S Kedwany; Hoda H Baghdadi

    2004-01-01

    Cadmium is a well-known human carcinogen and a potent nephrotoxin. Lipid peroxidation is involved in cadmium-related toxicity. Vitamin E and ?-carotene are effective antioxidants and free radical scavengers. Therefore, the present study was carried out to investigate the potential protective effects of vitamin E and ?-carotene alone or in combination against cadmium (Cd) toxicity. Cadmium chloride (CdCl2, 5 mg\\/kg BW,

  18. The rise of blood sugar as parameter for the degree of severity of hemorrhagic shock in the rabbit

    Microsoft Academic Search

    D. Holzrichter; A. Burk; U. Korn; R. Burk

    1983-01-01

    Summary  Besides the known cardiovascular effects of hemorrhagic shock, the following regular metabolic consequences can be demonstrated\\u000a in rabbits:\\u000a \\u000a \\u000a 1. \\u000a \\u000a The rise in blood sugar in hemorrhagic shock increases with the amount of blood withdrawn per unit time.\\u000a \\u000a \\u000a \\u000a \\u000a 2. \\u000a \\u000a The rise in blood sugar is independent of the state of consciousness or Nembutal anesthesia.\\u000a \\u000a \\u000a \\u000a \\u000a 3. \\u000a \\u000a The characteristic rise in blood sugar

  19. SLOPPINESS, MODELING, AND EVOLUTION IN BIOCHEMICAL NETWORKS

    E-print Network

    Sethna, James P.

    SLOPPINESS, MODELING, AND EVOLUTION IN BIOCHEMICAL NETWORKS A Dissertation Presented to the Faculty #12;SLOPPINESS, MODELING, AND EVOLUTION IN BIOCHEMICAL NETWORKS Ryan Nicholas Gutenkunst, Ph of such models to parameter changes are generically `sloppy', with eigenvalues roughly evenly spaced over many

  20. [Biochemical diagnostics of fatal opium intoxication].

    PubMed

    Papyshev, I P; Astashkina, O G; Tuchik, E S; Nikolaev, B S; Cherniaev, A L

    2013-01-01

    Biochemical diagnostics of fatal opium intoxication remains a topical problem in forensic medical science and practice. We investigated materials obtained in the course of forensic medical expertise of the cases of fatal opium intoxication. The study revealed significant differences between myoglobin levels in blood, urine, myocardium, and skeletal muscles. The proposed approach to biochemical diagnostics of fatal opium intoxication enhances the accuracy and the level of evidence of expert conclusions. PMID:23802303

  1. The Effect of Different Levels of Organic and Inorganic Chromium Supplementation on Production Performance, Carcass Traits and Some Blood Parameters of Broiler Chicken Under Heat Stress Condition

    Microsoft Academic Search

    Mohammad Mehdi Moeini; Akram Bahrami; Shahab Ghazi; Mohammad Reza Targhibi

    A total of 250 broilers in a completely randomized design selected to evaluate the effect of different levels of chromium\\u000a (Cr) supplementation on performance, carcass traits and some blood parameters of heat-stressed broiler chicks. All birds were\\u000a kept under heat stress temperature (33?±?3°C) and divided into five treatments groups. Each treatment consisted of five pens\\u000a with 10 birds in each

  2. Effect of Varying Levels of Turmeric Rhizome Powder on Some Blood Parameters of Broiler Chickens Fed Corn-Soybean Meal Based Diets

    Microsoft Academic Search

    M. Emadi; H. Kermanshahi; E. Maroufyan

    2007-01-01

    An experiment was conducted to study the effect of Turmeric Rhizome Powder (TRP) on some blood parameters in broiler chickens. A corn-soybean meal based diet containing levels of TRP (0.00, 0.25, 0.50 and 0.75%) in a completely randomized design with 4 treatments and 5 replicates of 10 birds each were fed to 200 day-old Ross male broiler chickens from 0-49

  3. The effect of heat load on nutrient utilization and blood parameters of Awassi ram lambs fed different types and levels of forages

    Microsoft Academic Search

    N. Denek; A. Can; S. Tufenk; K. Yazgan; H. Ipek; M. Iriadam

    2006-01-01

    This study was carried out to determine the season effect (heat load) on nutrient utilization and blood-rumen parameters of Awassi ram lambs fed different levels and types of forages. Total sixteen 2-year-old Awassi ram lambs (44.3±1.5kg) for each apparent digestion trials were used as experimental animals. Experiment was arranged in 2×2×2 factorial in which season (summer versus fall), forage type

  4. Effect of exposure and withdrawal of 900-MHz-electromagnetic waves on brain, kidney and liver oxidative stress and some biochemical parameters in male rats.

    PubMed

    Ragy, Merhan Mamdouh

    2014-04-01

    Abstract Increasing use of mobile phones in daily life with increasing adverse effects of electromagnetic radiation (EMR), emitted from mobile on some physiological processes, cause many concerns about their effects on human health. Therefore, this work was designed to study the effects of exposure to mobile phone emits 900-MHz EMR on the brain, liver and kidney of male albino rats. Thirty male adult rats were randomly divided into four groups (10 each) as follows: control group (rats without exposure to EMR), exposure group (exposed to 900-MHz EMR for 1?h/d for 60?d) and withdrawal group (exposed to 900-MHz electromagnetic wave for 1?h/d for 60?d then left for 30?d without exposure). EMR emitted from mobile phone led to a significant increase in malondialdehyde (MDA) levels and significant decrease total antioxidant capacity (TAC) levels in brain, liver and kidneys tissues. The sera activity of alanine transaminase (ALT), aspartate aminotransferase (AST), urea, creatinine and corticosterone were significantly increased (p?biochemicals changes and oxidative stress in brain, liver and renal tissue of albino rats. These alterations were corrected by withdrawal. PMID:24712749

  5. Effects of processing of starter diets on performance, nutrient digestibility, rumen biochemical parameters and body measurements of brown swiss dairy calves.

    PubMed

    Nejad, J Ghassemi; Torbatinejad, N; Naserian, A A; Kumar, S; Kim, J D; Song, Y H; Ra, C S; Sung, K I

    2012-07-01

    In order to investigate the effect of physical forms of starter diets on performance, weaning age, nutrient digestibility and rumen biochemical factors, 24 female of neonatal Brown Swiss calves (average body weight of 39.5±1.2 kg) were randomly assigned to three treatments. Dietary treatments were mashed (MS), pelleted (PS), and texturized (TS) starter using 8 calves from birth till 90 days of age in each treatment. Diets were formulated to be iso-nitrogenous with 21% crude protein. Based on the experimental results, calves that received PS and TS diets, had significant higher average daily gain (ADG) than those receiving MS (p<0.01). Dry matter intake in calves fed PS and TS was greater than calves fed MS (p<0.05), but there was no significant difference in feed efficiency. Treatments had no effect on initiation of rumination. Weaning age of calves in MS was longer than the other two treatments (p<0.05). Crude protein and organic matter digestibility in MS treated calves were lower than other treatments (p<0.05). No differences were observed in neutral detergent fiber (NDF) and ash digestibility among treatments (p>0.05). Ruminal pH was higher (p<0.01) in MS than the other groups, but ruminal ammonia (g/dl) concentration was not different among the treatments. Body measurements such as body length, pin width, hip width, pin to hip length, size of metacarpus and metatarsus bones, hip height, wither height, stomach size and heart girth were not significantly different among the treatments. Overall, it is concluded that starter diets in the form of pellet and texture can improve performance in neonatal Brown Swiss calves compared to the mashed form. PMID:25049653

  6. Effects of food processing and fibre content on the digestibility, energy intake and biochemical parameters of Blue-and-gold macaws (Ara ararauna L. - Aves, Psittacidae).

    PubMed

    Veloso, R R; Sakomura, N K; Kawauchi, I M; Malheiros, E B; Carciofi, A C

    2014-04-01

    Considering the increased incidence of obesity and metabolic diseases in caged psittacines, the effect of fibre and food processing was evaluated in the Blue-and-gold macaw. Four food formulations (0%, 7%, 14% and 21% of sugarcane fibre) processed by pelleting or extrusion were studied, resulting in eight diets. To study digestibility, 48 macaws housed in pairs in cages was used in a block design. Subsequently, diets containing 0% or 21% sugarcane fibre, pelleted or extrude was fed for 4 months to evaluate energy intake and blood metabolites. A 2 × 2 × 2 (two fibre levels, two food processing methods and two genders) factorial arrangement with subplots (beginning and end) was used. When differences were detected in anova's F test, data were submitted to polynomial contrasts in the first experiment and to orthogonal contrasts in the second experiment (p < 0.05). Fibre addition reduced protein, fat and energy (p < 0.001) digestibility in both food processing. Pelleted foods presented higher dry matter digestibility and food metabolisable energy (ME) than the extruded ones (p < 0.05). Fibre addition or the type of processing did not change ME ingestion (p > 0.05). The macaws gained body weight (p < 0.05) regardless of the diet (p > 0.05), but females fed with the high-fibre diets did not gain weight (p > 0.05), suggesting a low food ME (12.5 kJ/g).The substitution of the original diet (sunflower seeds, fruits and cooked maize) by the experimental foods decreased the basal (12-h fast) concentrations of glucose, cholesterol and triglycerides (p < 0.001). The consumption of pelleted diets reduced serum glucose and cholesterol (p < 0.05). Results suggest that the pelleted diets were more beneficial and can be used to reduce blood metabolites related to metabolic disorders that are commonly observed in macaws. PMID:23627662

  7. A longitudinal study to assess the impact of exercise on clinical, biochemical, and anthropometric parameters among the type 2 diabetes patients of South India

    PubMed Central

    Sukla, Pradeep; Shrivastava, Saurabh RamBihariLal; Shrivastava, Prateek Saurabh

    2015-01-01

    Aim: Aim of the study isto assess the scope of physical activity among type 2 diabetes mellitus (T2DM) patients residing in an urban area of SouthIndia. Materials and Methods: A longitudinal interventional study was conducted for a period of 8months (January 2014–August 2014) among patients of T2DM accessing the healthcare services at an Urban Health and Training Center (UHTC) of a Medical College located in Kancheepuram district, Tamil Nadu. Universal sampling method was employed and all diagnosed T2DM patients attending UHTC during the month of January were enrolled in the study provided they satisfied the inclusion and exclusion criteria. Overall 151 participants were considered for final analysis and all of them were subjected to indulge in moderate type of aerobic exercise in the form of brisk walking for 2.5 h spread over a period of 1 week, for the next 6months. Before and after the exercise regimen, cases were subjected to clinical evaluation, measurement of weight and waist circumference, and estimation of fasting and postprandial blood sugar; and the results were compared with the baseline estimates. The associations were tested using paired t-test for continuous (mean ± standard deviation ( SD)) variables. Results: Majority (86, 57%) of the diabetics were from the age group of 40–60 years with a definite female preponderance (82/151) in contrast to males (69/151). A significant reduction in both fasting and postprandial sugar was observed before and after the exercise. However, from the perspective of the weight and waist circumference, although a reduction was observed, the association was not statistically significant. Conclusion: The findings of the present longitudinal study suggest that compliance with moderate type of physical activity can significantly improve the fasting and postprandial blood sugar, apart from reduction in weight of T2DM patients. PMID:25625085

  8. Effects of Acoustic Parameters and Ultrasound Contrast Agent Dose on Focused-Ultrasound Induced Blood-Brain Barrier Disruption

    Microsoft Academic Search

    Nathan McDannold; Natalia Vykhodtseva; Kullervo Hynynen

    2008-01-01

    Previously, it was shown that low-intensity focused ultrasound pulses applied along with an ultrasound contrast agent results in temporary blood-brain barrier (BBB) disruption. This effect could be used for targeted drug delivery in the central nervous system. This study examined the effects of burst length, pulse repetition frequency (PRF), and ultrasound contrast agent dose on the resulting BBB disruption. One

  9. The influence of the luteal and follicular phases on major pharmacokinetic parameters of blood and breath alcohol kinetics in women

    Microsoft Academic Search

    Andrea Dettling; Astrid Preiss; Gisela Skopp; Hans-Thomas Haffner

    2010-01-01

    Drink tests involving 14 women were carried out to determine the effects of the menstrual cycle phases on the pharmacokinetics of ethanol. One experiment was carried out in the follicular phase of the cycle and another in the luteal phase, with the estradiol, progesterone, and testosterone levels being determined in both cases. The target concentration was a final blood alcohol

  10. Feeding Micronized and Extruded Flaxseed to Dairy Cows: Effects on Blood Parameters and Milk Fatty Acid Composition

    Microsoft Academic Search

    C. Gonthier; A. F. Mustafa; D. R. Ouellet; P. Y. Chouinard; R. Berthiaume; H. V. Petit

    2005-01-01

    Four lactating Holstein cows fitted with ruminal and duodenal cannulas were used in a 4 × 4 Latin square design to determine the effects of feeding micronized and extruded flaxseed on milk composition and blood profile in late lactation. Four diets were formulated: a control (C) diet with no flaxseed, a raw flaxseed (RF) diet, a micronized flaxseed (MF) diet,

  11. [Calculation of parameters and accurate control with real-time compensator in drive system of pulsatile blood pump].

    PubMed

    Cao, Yun; Pan, Gang; Zang, Wangfu; Bai, Jingfeng

    2011-03-01

    This article introduces a new method using the servo motor which is controlled by ARM microcontroller to provide power for a pulsatile blood pump to beat. This method is featured with straightforward structure, accurate control, excellent timeliness, stable performance and small noise. And it can adjust the rate of beat, the rate of flow and the compression ratio according to actual demand. PMID:21706790

  12. PM2.5 EXPOSURE CHANGES HEART RATE VARIABILITY (HRV) AND BLOOD PARAMETERS IN STATE HIGHWAY PATROL TROOPERS

    EPA Science Inventory

    Epidemiological studies show an association between ambient particulate matter PM and cardiovascular mortality. Panel and controlled exposure studies report PM-associated changes in HRV and blood factors involved in clotting and inflammation. We investigated the effects of in-veh...

  13. The Effect of Organic Solvents and Other Parameters on Trypsin-Catalyzed Hydrolysis of Na-Benzoyl-arginine-p-nitroanilide. A Project-Oriented Biochemical Experiment

    Microsoft Academic Search

    L. C. Correia; A. C. Bocewicz; S. A. Esteves; M. G. Pontes; L. M. Versieux; S. M. R. Teixeira; M. M. Santoro; M. P. Bemquerer

    2001-01-01

    The study of enzymatic catalysis is a classical biochemistry experiment for undergraduate classes. We propose the utilization of the serine protease trypsin to discuss several parameters affecting enzyme catalysis. Hydrolysis of the chromogenic substrate Na -benzoyl-arginine-p-nitroanilide (BApNA) was followed by spectrophotometric monitoring. The optimal pH and temperature values were found to be 8.0 and 40 °C, respectively. Km and Vmax

  14. [Evaluation of functional state of crayfish Pontastacus leptodactylus in normal and toxic environment by characteristics of their cardiac activity and hemolymph biochemical parameters].

    PubMed

    Kuznetsova, T V; Sladkova, G V; Kholodkevich, S V

    2010-01-01

    The work deals with study of basic characteristics of cardiac activity (HR and stress-index) and hemolymph protein parameters in the crayfish Pontastacus leptodactylus. The main criteria of the crayfish selection are developed for formation of the animal reference groups available for subsequent toxicologic experiments. The action of a model toxicant, hydroquinone (often present in waste water), at a concentration of 1 g/l on parameters of the crayfish cardiac activity at various exposition time, its effect on circadian cardiac activity and on the hemolymph total protein were studied. At its short-term exposure, hydroquinone produced a temporary increase of HR and stress-index, but had no marked effect on the total protein parameters in hemolymph. At long action (for one day), hydroquinone led to a considerable tachycardia, disturbance of the circadian cardioactivity and to a decrease by 40% of the hemolymph total protein. In 50% of cases the toxicant caused death of the animals either in the course of its action or at period of washout from the toxicant. Mechanisms of the toxic action of hydroquinone at various levels of organization are discussed. PMID:20583580

  15. Heart rate variability, hemostatic and acute inflammatory blood parameters in healthy adults after short-term exposure to welding fume

    Microsoft Academic Search

    E. Scharrer; H. Hessel; A. Kronseder; W. Guth; B. Rolinski; R. A. Jörres; K. Radon; R. Schierl; P. Angerer; D. Nowak

    2007-01-01

    The present study aimed to investigate, whether short-term experimental exposure to high levels of welding fumes would be\\u000a capable of exerting acute effects in healthy subjects. Specifically, we assessed cardiovascular function in terms of heart\\u000a rate variability (HRV) as well as the concentrations of inflammatory mediators and hemostatic proteins in blood as outcome\\u000a measures. Twenty subjects without a history of

  16. Honey ameliorates influence of hemorrhage and food restriction on renal and hepatic functions, and hematological and biochemical variables.

    PubMed

    Al-Waili, Noori S; Saloom, Khelod Y; Akmal, Mohammod; Al-Waili, Faiza; Al-Waili, Thia N; Al-Waili, Ali N; Ali, Amjed

    2006-01-01

    The objectives were to assess the effects of various diets, including total food restriction with 50% honey feeding, total food restriction with 50% dextrose feeding or adlibitum (control group) commercial regular diet, on the hematology and biochemical variables, and to assess the effects of the various diets on the influence of acute blood loss on the same parameters. Thirty Sprague-Dawley albino rats were divided into three groups, 10 rats each: group A, fed a commercial regular diet; group B, total food restriction with 50% dextrose feeding; and group C, total food restriction with 50% honey feeding. After 8 days of feeding, rats were subjected to acute blood loss (6 ml/kg) and blood investigations were performed. After acute blood loss, the same feedings were continued for a further 8 days and the blood tests were repeated at day 8 post-bleeding. Total food restriction with 50% dextrose feeding compared with commercial regular diet reduces hematological and biochemical variables. Total food restriction with 50% honey feeding compared with total food restriction with 50% dextrose feeding causes a greater reduction in fasting blood glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and triacylglycerol. Acute blood loss causes elevation of white blood cells, lymphocyte percentage, fasting blood sugar, blood urea nitrogen, alkaline phosphatase and triacylglycerol, and a reduction in serum albumen, protein, cholesterol, AST, serum creatinine and hemoglobin; the results are significant (P<0.05) concerning fasting blood glucose, AST, alkaline phosphatase, serum albumin and protein. A significant reduction in fasting blood glucose, white blood cells, BUN, AST, ALT, alkaline phosphatase and triacylglycerol, and a significant elevation of hemoglobin and serum albumin are obtained after acute blood loss in rats on total food restriction with 50% honey feeding as compared with the other two groups. Total food restriction with 50% honey feeding increases serum albumin, serum protein, fasting blood glucose, and causes lower reduction in hemoglobin as compared with the other groups. Conclusively, honey feeding during total food restriction significantly modifies and ameliorates biochemical and hematological changes observed after acute blood loss. This will pave the way to use honey as part of bleeding management and during a food restriction regimen. PMID:17135025

  17. Single and combined effects of vitamin C and oregano essential oil in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress condition.

    PubMed

    Ghazi, Shahab; Amjadian, Tahere; Norouzi, Shokufeh

    2014-10-22

    This study was conducted to evaluate the effects of adding vitamin C (VC), oregano essential oil (OR), or their combination in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress (HS) condition (38 °C). One-day-old 240 male broilers were randomly assigned to four treatment groups, six replicates of ten birds each. The birds were fed with either a basal diet or a basal diet supplemented with either 200 mg L-ascorbic acid/kg of diet, 250 mg of oregano essential oil/kg of diet, or 200 mg L-ascorbic acid plus 250 mg of oregano essential oil/kg of diet. Average daily feed intake (ADFI), average daily gain (ADG), and feed conversion ratio (FCR) were obtained for 42 days of age and at the end of the experiment (day 42); birds were bled to determine some blood parameters and weighted for final body weight (BW). Feeding birds with diets supplemented with oregano essential oil and vitamin C in a single or combined form increased ADG (P?>?0.05). Also BW increased and feed efficiency decreased (P??0.05). Supplemental oregano essential oil and vitamin C in a combined form decreased the serum concentration of corticosterone, triglycerides, glucose, and MDA (P?blood parameters of broiler chicks reared under heat stress condition. PMID:25336108

  18. Drugs Modifying Nitric Oxide Metabolism Affect Plasma Cholesterol Levels, Coagulation Parameters, Blood Pressure Values and the Appearance of Plasma Myocardial Necrosis Markers in Rabbits: Opposite Effects of L-NAME and Nitroglycerine

    Microsoft Academic Search

    Arnaldo Pinelli; Silvio Trivulzio; Livio Tomasoni; Boris Bertolini; Sergio Brenna; Edgardo Bonacina; Roberto Accinni

    2003-01-01

    Various experiments have shown that decreased nitric oxide values alter plasma lipid levels or coagulation parameters or blood pressure values or cause myocardial necrosis phenomena, but it is not clear whether these alterations are reciprocally connected, or whether nitric oxide changes are involved in the appearance of some coronary disease risk factors (lipid, coagulation, blood pressure alterations) and myocardial necrosis.

  19. The biochemical properties of the pulmonary circulation

    Microsoft Academic Search

    J. A. Pang; D. M. Geddes

    1981-01-01

    The biochemical properties of the pulmonary circulation are reviewed, with special reference to the fate of biologically active\\u000a substances in the blood. Possible physiological, pathological, pharmacological and practical implications of clinical relevance\\u000a are discussed.

  20. Effects of an aqueous extract of Aloe buettneri, Justicia insularis, Hibiscus macranthus, Dicliptera verticillata on some physiological and biochemical parameters of reproduction in immature female rats.

    PubMed

    Telefo, P B; Moundipa, P F; Tchana, A N; Tchouanguep Dzickotze, C; Mbiapo, F T

    1998-12-01

    Different doses of aqueous extracts from the leaves of Aloe buettneri, Justicia insularis, Hibiscus macranthus and Dicliptera verticillata, locally used to regulate the menstrual cycle and to treat dysmenorrhea or infertility in women, were given daily to 22 day old rats for 5, 10, 15, 20 and 25 days by gastric intubation. At the end of each experimental period, the weights of ovaries and uteri, levels of uterine and ovarian proteins, ovarian cholesterol and serum oestradiol were evaluated. The results showed a decrease in growth rate of animals treated with 94 mg/kg per day at the end of the experimental period. The ovarian and uterine weights were high in all treated groups especially within the pubertal period (36-41 days old) when compared to the respective controls. During the same period, the parameters analysed showed significant differences in treated rats when compared to the controls. This was seen with ovarian and uterine protein levels, as well as with serum oestradiol whose level was especially high in the groups given 49 or 94 mg/kg per day of the plant extracts (52 and 42%, respectively when compared with the respective controls). A concomitant decrease in ovarian cholesterol was observed in the same treated groups. These results suggested a possible presence of oestrogenic compounds in the plant extracts. PMID:10030723

  1. Reagent-free monitoring of multiple clinically relevant parameters in human blood plasma using a mid-infrared quantum cascade laser based sensor system.

    PubMed

    Brandstetter, Markus; Sumalowitsch, Tamara; Genner, Andreas; Posch, Andreas E; Herwig, Christoph; Drolz, Andreas; Fuhrmann, Valentin; Perkmann, Thomas; Lendl, Bernhard

    2013-07-21

    We present a semi-automated point-of-care (POC) sensor approach for the simultaneous and reagent-free determination of clinically relevant parameters in blood plasma. The portable sensor system performed direct mid-infrared (MIR) transmission measurements of blood plasma samples using a broadly tunable external-cavity quantum cascade laser source with high spectral power density. This enabled the use of a flow cell with a long path length (165 ?m) which resulted in high signal-to-noise ratios and a rugged system, insensitive to clogging. Multivariate calibration models were built using well established Partial-Least-Squares (PLS) regression analysis. Selection of spectral pre-processing procedures was optimized by an automated evaluation algorithm. Several analytes, including glucose, lactate, triglycerides, cholesterol, total protein as well as albumin, were successfully quantified in routinely taken blood plasma samples from 67 critically ill patients. Although relying on a spectral range from 1030 cm(-1) to 1230 cm(-1), which is optimal for glucose and lactate but rather unusual for protein analysis, it was possible to selectively determine the albumin and total protein concentrations with sufficient accuracy for POC application. PMID:23678484

  2. Effects of nickel-chrome dental alloys used in dentistry on saliva and serum nickel levels, peripheral T-lymphocytes and some other blood parameters.

    PubMed

    Arikan, A

    1992-07-01

    Epidemiological investigations have shown that nickel is a potent sensitivity producing agent. However, it is not known if intra-oral use of nickel-chrome alloys can cause any sensitization reaction. The purpose of this study was to determine if nickel-chrome alloys used in dentistry influence nickel levels in saliva and serum and whether there were associated changes of some blood parameters such as lymphocyte, T-lymphocyte, neutrophil, eosinophil, basophil and monocyte. The study was performed on 10 patients all of whom had no medical and dental history of using nickel containing drugs or restorations. Four unit fixed partial prostheses containing 22.97% nickel and 22.65% chrome were constructed and worn by the patients. Blood and unstimulated whole saliva samples were taken from the patients before insertion of the prostheses and then 1 month and 6 months post-insertion. Hematocrit determinations, total and differential leucocyte count, total T-lymphocyte counts and serum nickel concentrations were determined from the blood samples and salivary nickel concentrations from the saliva samples. It was found that there were no significant changes in circulating T-lymphocyte and monocyte population but a decrease was found in circulating eosinophil and an increase in circulating neutrophil and basophil populations. PMID:1432350

  3. Alterations in cell surface area and deformability of individual human red blood cells in stored blood

    E-print Network

    Park, HyunJoo; Lee, SangYun; Kim, Kyoohyun; Sohn, Yong-Hak; Jang, Seongsoo; Park, YongKeun

    2015-01-01

    The functionality and viability of stored human red blood cells (RBCs) is an important clinical issue in transfusion. To systematically investigate changes in stored whole blood, the hematological properties of individual RBCs were quantified in blood samples stored for various periods with and without a preservation solution called CPDA-1. With 3-D quantitative phase imaging techniques, the optical measurements of the 3-D refractive index (RI) distributions and membrane fluctuations were done at the individual cell level. From the optical measurements, the morphological (volume, surface area and sphericity), biochemical (hemoglobin content and concentration), and mechanical parameters (dynamic membrane fluctuation) were simultaneously quantified to investigate the functionalities and their progressive alterations in stored RBCs. Our results show that the stored RBCs without CPDA-1 had a dramatic morphological transformation from discocytes to spherocytes within 2 weeks which was accompanied with significant ...

  4. Genotoxic effects of heavy metal cadmium on growth, biochemical, cyto-physiological parameters and detection of DNA polymorphism by RAPD in Capsicum annuum L. – An important spice crop of India

    PubMed Central

    Aslam, Rumana; Ansari, M.Y.K.; Choudhary, Sana; Bhat, Towseef Mohsin; Jahan, Nusrat

    2014-01-01

    The present study was designed to investigate the effects of cadmium (Cd) on biochemical, physiological and cytological parameters of Capsicum annuum L. treated with five different concentrations (20, 40, 60, 80 and 100 ppm) of the metal. Shoot–root length, pigment and protein content showed a continuous decrease with increasing Cd concentrations and the maximal decline was observed at the higher concentration. Proline content was found to be increased upto 60 ppm while at higher concentrations it gradually decreased. MDA content and chromosomal aberrations increased as the concentration increased. Additionally Random amplified polymorphic DNA (RAPD) technique was used for the detection of genotoxicity induced by Cd. A total of 184 bands (62 polymorphic and 122 monomorphic) were generated in 5 different concentrations with 10 primers where primer OPA-02 generated the highest percentage of polymorphism (52.63%). Dendrogram showed that control, R1 and R2 showed similar cluster and R4 and R5 grouped with R3 into one cluster, which showed that plants from higher doses showed much difference than the plants selected at mild doses which resemble control at the DNA level. This investigation showed that RAPD marker is a useful tool for evaluation of genetic diversity and relationship among different metal concentrations. PMID:25313282

  5. Effects of Aqueous Extract of Triplochiton scleroxylon on Red Blood Cells and Associated Parameters in Alloxan - Induced Diabetic Rabbits

    Microsoft Academic Search

    2006-01-01

    2 Abstract: The effect of aqueous extract of Triplochiton scleroxylon on the body weight, plasma glucose concentration and some hematological parameters in alloxan-induc ed diabetic rabbits were investigated. Rabbits of the same strain (New Zealand) weighing between 1.20 and 1.68kg were used. At least 100ml of the aqueous extract of the plant was administered to a set of test rabbits

  6. A Method for Generate a Mouse Model of Stroke: Evaluation of Parameters for Blood Flow, Behavior, and Survival

    PubMed Central

    Park, Sin-Young; Marasini, Subash; Kim, Geu-Hee; Ku, Taeyun; Choi, Chulhee; Park, Min-Young; Kim, Eun-Hee; Lee, Young-Don; Suh-Kim, Haeyoung

    2014-01-01

    Stroke is one of the common causes of death and disability. Despite extensive efforts in stroke research, therapeutic options for improving the functional recovery remain limited in clinical practice. Experimental stroke models using genetically modified mice could aid in unraveling the complex pathophysiology triggered by ischemic brain injury. Here, we optimized the procedure for generating mouse stroke model using an intraluminal suture in the middle cerebral artery and verified the blockage of blood flow using indocyanine green coupled with near infra-red radiation. The first week after the ischemic injury was critical for survivability. The survival rate of 11% in mice without any treatment but increased to 60% on administering prophylactic antibiotics. During this period, mice showed severe functional impairment but recovered spontaneously starting from the second week onward. Among the various behavioral tests, the pole tests and neurological severity score tests remained reliable up to 4 weeks after ischemia, whereas the rotarod and corner tests became less sensitive for assessing the severity of ischemic injury with time. Further, loss of body weight was also observed for up 4 weeks after ischemia induction. In conclusion, we have developed an improved approach which allows us to investigate the role of the cell death-related genes in the disease progression using genetically modified mice and to evaluate the modes of action of candidate drugs. PMID:24737945

  7. Hematological and plasma biochemical reference ranges of Alaskan seabirds: Their ecological significance and clinical importance

    USGS Publications Warehouse

    Newman, S.H.; Piatt, J.F.; White, J.

    1997-01-01

    Blood was analyzed from 151 pelagic marine birds to establish reference ranges for hematological and plasma biochemical parameters from healthy, wild populations of Pacific seabirds. Of the 13 species examined, 9 were from the Family Alcidae (N = 122 individuals) and the remainder (N = 29) from the Families Phalacrocoracidae, Laridae, and Procellariidae. Three of 8 hematological parameters (total white blood cell count, lymphocyte count and eosinophil count) differed significantly among species, as did 9 of 13 plasma biochemical parameters (alkaline phosphatase, aspartate aminotransferase, creatine kinase, cholesterol, glucose, lactate dehydrogenase, total bilirubin, total protein and field total protein). There were no differences among species for packed cell volume, buffy coat, cell counts of heterophils, monoqtes and basophils, or for concentrations of alanine aminotransferase, triglycerides, uric acid and calcium. Plasma calcium concentration, triglyceride levels and field total protein varied significantly between sexes, with females having higher mean concentrations of all 3 parameters. However, no significant relationships between measures of breeding condition (brood patch size, subcutaneous and mesenteric fat deposits, or ovarian follicle size and ovary weight) and calcium or alkaline phosphatase concentrations in female birds could be identified. Alanine aminotransferase and uric acid were the only analytes which did not differ significantly between species or sexes.

  8. Investigation of biochemical and haematological side-effects of cefquinome in healthy dogs.

    PubMed

    Maden, M; Tra?, B; Ba?, A L; Elmas, M; Yazar, E; Birdane, F M

    2001-01-01

    In the present study, the effects of cefquinome, a 4th generation cephalosporin, on clinical, biochemical, haematological, and blood gas variables were investigated. Five healthy dogs were injected with cefquinome (1 mg/kg body weight, IM, daily) for 14 days. Negative effects of cefquinome on clinical, biochemical, and haematological variables were not observed, but it did change some blood gas variables. PMID:11205999

  9. Pharmacology: Investigation of biochemical and haematological side?effects of cefquinome in healthy dogs

    Microsoft Academic Search

    M. Maden; B. Tra?; A. L. Ba?; M. Elmas; E. Yazar; F. M. Birdane

    2001-01-01

    In the present study, the effects of cefquinome, a 4 generation cephalosporin, on clinical, biochemical, haemato?logical, and blood gas variables were investigated. Five healthy dogs were injected with cefquinome (1 mg\\/kg body weight, IM, daily) for 14 days. Negative effects of cefquinome on clinical, biochemical, and haematological variables were not observed, but it did change some blood gas variables.

  10. Effect of exposure to operant-controlled microwaves on certain blood and immunological parameters in the young chick

    SciTech Connect

    Braithwaite, L.A.; Morrison, W.D.; Bate, L.; Otten, L.; Hunter, B.; Pei, D.C. (Department of Animal and Poultry Science, University of Guelph, Ontario (Canada))

    1991-03-01

    Twenty-two 1-wk-old broiler chicks (Gallus domesticus) were housed at 16 C and operantly conditioned to activate either a 250-W infrared bulb (control) or a microwave generator delivering 13 mW/cm2 (treated). Plasma corticosterone concentration did not differ between groups (P greater than .05) at 4 wk of age. At that time the birds were killed, and post-mortem examination revealed no treatment differences in gross morphology of the chicks or in weights of spleen and bursa of Fabricius (P greater than .05). Histological study of comparable segments of spleen, bursa, adrenal, and thyroid tissue did not show differences in any of the chosen parameters (P greater than .05). Heterophil:lymphocyte ratios, packed cell volume, and total plasma protein content were similar between groups (P greater than .05). These results suggest that operant exposure to low density microwave radiation did not result in stress or immunological disturbances.

  11. Dietary nitrate modulates cerebral blood flow parameters and cognitive performance in humans: A double-blind, placebo-controlled, crossover investigation.

    PubMed

    Wightman, Emma L; Haskell-Ramsay, Crystal F; Thompson, Kevin G; Blackwell, Jamie R; Winyard, Paul G; Forster, Joanne; Jones, Andrew M; Kennedy, David O

    2015-10-01

    Nitrate derived from vegetables is consumed as part of a normal diet and is reduced endogenously via nitrite to nitric oxide. It has been shown to improve endothelial function, reduce blood pressure and the oxygen cost of sub-maximal exercise, and increase regional perfusion in the brain. The current study assessed the effects of dietary nitrate on cognitive performance and prefrontal cortex cerebral blood-flow (CBF) parameters in healthy adults. In this randomised, double-blind, placebo-controlled, parallel-groups study, 40 healthy adults received either placebo or 450ml beetroot juice (~5.5mmol nitrate). Following a 90minute drink/absorption period, participants performed a selection of cognitive tasks that activate the frontal cortex for 54min. Near-Infrared Spectroscopy (NIRS) was used to monitor CBF and hemodynamics, as indexed by concentration changes in oxygenated and deoxygenated-haemoglobin, in the frontal cortex throughout. The bioconversion of nitrate to nitrite was confirmed in plasma by ozone-based chemi-luminescence. Dietary nitrate modulated the hemodynamic response to task performance, with an initial increase in CBF at the start of the task period, followed by consistent reductions during the least demanding of the three tasks utilised. Cognitive performance was improved on the serial 3s subtraction task. These results show that single doses of dietary nitrate can modulate the CBF response to task performance and potentially improve cognitive performance, and suggest one possible mechanism by which vegetable consumption may have beneficial effects on brain function. PMID:26037632

  12. Effect of bioactive substances found in rapeseed, raspberry and strawberry seed oils on blood lipid profile and selected parameters of oxidative status in rats.

    PubMed

    Pieszka, Marek; Tombarkiewicz, Barbara; Roman, Adam; Migda?, W?adys?aw; Niedzió?ka, Jerzy

    2013-11-01

    Rapeseed, strawberry and raspberry seed oils are a rich source of polyunsaturated fatty acids and antioxidants such as tocols, bioflavonoids and phytosterols. The aim of the study was to determine changes in the blood lipid profile of rats fed with rapeseed, strawberry and raspberry seed oils and their effects on selected parameters of oxidative status. The experiment was carried out on male Wistar rats. The oils were administered by oral gavage for 5 weeks once daily at the dose of about 0.8 ml per rat. Blood samples were taken before and after supplementation period. The activity of superoxide dismutase (SOD) and glutathione peroxidase (cGPx) was assessed in erythrocytes and contents of triglycerides (TG), total cholesterol, low-density fraction of cholesterol (LDL) and high-density fraction of cholesterol (HDL) were assessed in plasma. The experiment shows that oils supplemented in the diet for 5 weeks had no significant effect on the level of triglyceride (TG), total cholesterol as well as HDL and LDL fractions. Reduced activity of cGPX and SOD in the group of rats receiving raspberry and strawberry seed oils suggests that these native oils may contribute to oxidative stability (improves antioxidant status). Thus, strawberry and raspberry seed oils can be considered as special biological oils, which constitute potential nutraceuticals reducing oxidative stress. PMID:24121557

  13. An experiment on the pulsatile flow at transitional Reynolds numbers--the fluid dynamical meaning of the blood flow parameters in the aorta.

    PubMed

    Nakamura, M; Sugiyama, W; Haruna, M

    1993-11-01

    An experiment on the fully developed sinusoidal pulsatile flow at transitional Reynolds numbers was performed to evaluate the basic characteristics of the wall shear stress. In this experiment, the wall shear stress was calculated from the measured section averaged axial velocity and the pressure gradient by using the section averaged Navier-Stokes equation. The experimental results showed that the ratio of the amplitude of the wall shear stress to the amplitude of the pressure gradient had the maximum value when the time averaged Reynolds number was about 4000 and the Womersley number was about 10. As this condition is close to the blood flow condition in the human aorta, it is suggested that the parameter of the aorta has an effect to increase the amplitude of the wall shear stress acting on the arterial wall. PMID:8309236

  14. Association between weight gain, blood parameters, and thyroid hormones and the development of ascites syndrome in broiler chickens.

    PubMed

    Luger, D; Shinder, D; Rzepakovsky, V; Rusal, M; Yahav, S

    2001-07-01

    The present study examined the association between thyroid hormones and the development of ascites on one hand and the ability to predict ascites from growth rate and hematocrit on the other hand. Ascites syndrome was induced in broiler chickens in two trials by exposing the chicks to low ambient temperature (Ta) and by supplying a pellet form of diet. Weight gain, hematocrit, hemoglobin, and plasma thyroxin (T4) and triiodothyronine (T3) concentrations were measured weekly for each bird, and comparisons were made between birds that eventually died from ascites and those that did not. Mortality from ascites amounted to 24.3 and 24.2% in Trials 1 and 2, respectively. Weight gain did not differ between ascitic and healthy chickens up to approximately 2 wk before death but was significantly lower in the ascitic broilers 1 to 2 wk before death. Hematocrit was significantly higher in broilers with ascites with the exception of ascitic broilers that died at the age of 7 wk (Trial 1). In ascitic broilers, T4 and T3 concentrations declined significantly during the week of death. The present findings raise the question of whether the association between low levels of thyroid hormones and the development of ascites is one of the physiological responses in the syndrome cascade, or whether the failure to maintain thyroid hormones concentration is one of the triggers of the syndrome initiation. This question requires further investigation. It can be concluded that a high rate of weight gain is not always a good predictor of ascites development. Hematocrit and thyroid hormones can provide a good indication but only during the last week of life, and not in all cases. None of these parameters, however, can predict the development of ascites at an early age. PMID:11469663

  15. Evaluation of clinicopathological parameters in cattle grazing timber milkvetch.

    PubMed

    Maricle, B; Tobey, J; Majak, W; Hall, J W

    1996-03-01

    The purpose of this study was to determine whether hematological and serum biochemical parameters could be used to diagnose poisoning in cattle grazing timber milkvetch (Astragalus miser var. serotinus), a nitropropanol-containing legume. Blood samples were obtained from 20 Hereford cattle on timber milkvetch range in southern British Columbia during 1992 and 1993. Relevant changes in blood parameters were not detected and the parameters could not be used to predict nitropropanol ingestion or intoxication. The same parameters were measured in dosing trials with cattle with rumen fistulas, but relevant changes were not detected. Succinate dehydrogenase is the key target in nitropropanol intoxication and an assay for this enzyme may provide a probe for diagnosis. PMID:8681281

  16. Evaluation of clinicopathological parameters in cattle grazing timber milkvetch.

    PubMed Central

    Maricle, B; Tobey, J; Majak, W; Hall, J W

    1996-01-01

    The purpose of this study was to determine whether hematological and serum biochemical parameters could be used to diagnose poisoning in cattle grazing timber milkvetch (Astragalus miser var. serotinus), a nitropropanol-containing legume. Blood samples were obtained from 20 Hereford cattle on timber milkvetch range in southern British Columbia during 1992 and 1993. Relevant changes in blood parameters were not detected and the parameters could not be used to predict nitropropanol ingestion or intoxication. The same parameters were measured in dosing trials with cattle with rumen fistulas, but relevant changes were not detected. Succinate dehydrogenase is the key target in nitropropanol intoxication and an assay for this enzyme may provide a probe for diagnosis. Images Figre 1. PMID:8681281

  17. Initial blood storage experiment

    NASA Technical Reports Server (NTRS)

    Surgenor, Douglas MACN.

    1988-01-01

    The possibility of conducting experiments with the formed elements of the blood under conditions of microgravity opens up important opportunities to improve the understanding of basic formed element physiology, as well as, contribution to improved preservation of the formed elements for use in transfusion. The physiological, biochemical, and physical changes of the membrane of the erythrocyte, platelet, and leukocyte was studied during storage under two specific conditions: standard blood bank conditions and microgravity, utilizing three FDA approved plastic bags. Storage lesions; red cell storage on Earth; platelet storage on Earth; and leukocyte storage Earth were examined. The interaction of biomaterials and blood cells was studied during storage.

  18. Biochemical transformation of coals

    DOEpatents

    Lin, M.S.; Premuzic, E.T.

    1999-03-23

    A method of biochemically transforming macromolecular compounds found in solid carbonaceous materials, such as coal is provided. The preparation of new microorganisms, metabolically weaned through challenge growth processes to biochemically transform solid carbonaceous materials at extreme temperatures, pressures, pH, salt and toxic metal concentrations is also disclosed. 7 figs.

  19. Biochemical transformation of coals

    DOEpatents

    Lin, Mow S. (Rocky Point, NY); Premuzic, Eugene T. (East Moriches, NY)

    1999-03-23

    A method of biochemically transforming macromolecular compounds found in solid carbonaceous materials, such as coal is provided. The preparation of new microorganisms, metabolically weaned through challenge growth processes to biochemically transform solid carbonaceous materials at extreme temperatures, pressures, pH, salt and toxic metal concentrations is also disclosed.

  20. Nickel-induced blood biochemistry alterations in hens after an experimental peroral administration

    Microsoft Academic Search

    Adriana Kolesarova; Marcela Capcarova; Henrieta Arpasova; Anna Kalafova; Peter Massanyi; Norbert Lukac; Jaroslav Kovacik; Monika Schneidgenova

    2008-01-01

    The purpose of this study was to determine certain blood biochemical parameters in hens of Isa Brown breed (n = 20) after nickel administration. Animals were divided into four groups (K, P1, P2, P3). Experimental hens (n = 5; in each group) received nickel (NiCl2) as peroral administration in drinking water in various doses (P1 – 0.02 g NiCl2\\/L; P2

  1. Formation of secondary messengers by blood-formed elements in low-power laser irradiation

    NASA Astrophysics Data System (ADS)

    Brill, Gregory E.; Proshina, Olga V.; Zhigalina, Valentina N.; Filimonovskaya, Lyudmila S.; Romanova, Tatyana P.; Petrisheva, Svetlana G.; Zolotarjova, Tamara M.

    1995-05-01

    Irradiation of heparinized rat blood by He-Ne laser light ((lambda) - 632.8 nm, power density - 5 mW/cm2) during 15 or 30 min was performed in vitro experiments. The complex of biochemical parameters of erythrocytes, plasma and cytochemical parameters of polymorphonuclear leucocytes was studied. Laser irradiation was stated to cause different metabolic changes in red blood cells and neutrophils depending on the dose. In both doses of irradiation glucose-6-phosphate dehydrogenase activity lowers in erythrocytes, succinate dehydrogenase activity and lysosomal cationic proteins content increase in neutrophils. Stimulation of oxygen active forms production in cellular membranes of blood formed elements results in plasma malonic dialdehyde level increase and in the change of the balance between primary and secondary lipid peroxidation products. Cooperative interaction between different blood cells in the process of realization of system response to laser exposure is supposed to exist.

  2. Effect of rare earth elements on beef cattle growth performance, blood clinical chemical parameters and mitogen stimulated proliferation of bovine peripheral blood mononuclear cells in vitro and ex vivo.

    PubMed

    Renner, Lydia; Schwabe, Annett; Döll, Susanne; Höltershinken, Martin; Dänicke, Sven

    2011-03-25

    Rare earth elements (REE) are possible performance enhancers in animal production, but little is known about their effects on ruminants. Therefore a feeding trial was conducted with 40 fattening bulls who received 0, 100, 200 or 300mg REE-citrate/kg dry matter (DM), containing 34.30% La, 58.09% Ce and 7.61% other REE. DM intake was measured daily and live weight weekly. Ex vivo ConcanavalinA (ConA)-stimulated cell proliferation of peripheral blood mononuclear cells (PBMC) was tested by MTT and alamar blue (AB) assay. Serum was analysed for clinical chemical parameters, ion (Mg, Ca and P) and REE concentrations. The effects of LaCl(3), CeCl(3), NdCl(3) and YCl(3) on ConA-stimulated proliferation of PBMC were tested in vitro, using MTT and AB assay. REE-citrate supplementation did affect DM intake, but not live weight gain, clinical chemical parameters, and ion concentrations significantly. In REE-300 group ex vivo proliferation of PBMC was significantly increased. In vitro ConA-stimulated proliferation decreased with rising REE-chloride concentrations. At least at the highest tested concentration (approximately 290?M) the inhibition reached significance. Proliferation of non-stimulated PBMC was not affected dose-dependently. REE affect the proliferation of PBMC, thus an effect on the bovine immune system is possible. However, the great differences in effective doses in vitro and ex vivo (serum REE concentrations) might explain the different results from the experiments. PMID:21256936

  3. Biochemical correlates of neurosensory changes in weightlessness

    NASA Technical Reports Server (NTRS)

    Leach, Carolyn S.; Reschke, Millard F.

    1989-01-01

    The possible existence of a relationship between space motion sickness and chemical and biochemical variables measured in body fluids is studied. Clinical chemistry and endocrine measurements from blood and urine samples taken before and after Space Shuttle flights were analyzed along with the occurrence of SMS during flight and provocative testing before flight. Significant positive correlations were observed with serum chloride and significant negative correlations with serum phosphate, serum uric acid, and plasma thyroid stimulating hormone.

  4. Potential biochemical markers for infantile autism

    Microsoft Academic Search

    Porn P. Israngkun; Howard A. I. Newman; Suman T. Patel; Valentine A. Duruibe; Hussein Abou-Issa

    1986-01-01

    Biochemical markers are crucial to the development of early diagnosis of infantile autism. The blood concentrations of neuroanalytes\\u000a epinephrine, norepinephrine, dopamine, and serotonin were elevated in autistic subjects (n = 13) as compared to normal controls (n = 10). Autistic subjects had peptide patterns (peaks I-V, Sephadex G-25) that were different from those of normal controls.\\u000a Methionine-enkephalin has been tentatively

  5. Effects of dietary selenium and vitamin E on immune response and biological blood parameters of broilers reared under thermoneutral or heat stress conditions

    NASA Astrophysics Data System (ADS)

    Habibian, Mahmood; Ghazi, Shahab; Moeini, Mohammad Mehdi; Abdolmohammadi, Alireza

    2014-07-01

    A study was conducted using 360 broiler chickens to evaluate the effects of dietary vitamin E (0, 125 and 250 mg/kg), selenium (Se, 0, 0.5 and 1 mg/kg), or their different combinations on immune response and blood biological parameters of broilers raised under either thermoneutral (TN, 23.9 °C constant) or heat stress (HS, 23.9 to 37 °C cycling) conditions. Humoral immunity was assessed by intravenous injection of 7 % sheep red blood cell (SRBC) followed by evaluation of serum for antibody titers in primary and secondary responses. Heterophil to lymphocyte (H/L) ratio also determined as an indicator of stress. Furthermore, at the end of the experiment, birds were bled for determination of some biological parameters. There was a significant reduction in body weight and feed intake, but the feed conversion ratio increased when the birds were exposed to HS ( P < 0.05). Body weight and feed intake were not influenced significantly by dietary vitamin E and Se ( P > 0.05), whereas feed conversion was improved significantly by 125 mg/kg vitamin E ( P < 0.05). The liver and lymphoid organ weights as well as IgM and IgG, antibody titers for primary and secondary antibody responses to SRBC were reduced significantly under HS ( P < 0.05). Heat stress also resulted in a significant increase in H/L ratio ( P < 0.05). Dietary vitamin E resulted in improvement of primary and secondary antibody responses both in TN and HS broilers ( P < 0.05). The HS birds also showed an improved antibody titer in secondary response with high concentration of Se ( P < 0.05). Vitamin E and Se had interactive effects on anti-SRBC titers; however, no consistent differences were found between dietary levels during the study. The H/L ratio decreased by feeding vitamin E at both levels either under HS or TN conditions ( P < 0.05). The serum concentrations of glucose, triglycerides, total cholesterol, and LDL-cholesterol were increased but serum HDL-cholesterol decreased in HS broilers ( P < 0.05).

  6. Red Cell Distribution Width and Other Red Blood Cell Parameters in Patients with Cancer: Association with Risk of Venous Thromboembolism and Mortality

    PubMed Central

    Riedl, Julia; Posch, Florian; Königsbrügge, Oliver; Lötsch, Felix; Reitter, Eva-Maria; Eigenbauer, Ernst; Marosi, Christine; Schwarzinger, Ilse; Zielinski, Christoph; Pabinger, Ingrid; Ay, Cihan

    2014-01-01

    Background Cancer patients are at high risk of developing venous thromboembolism (VTE). Red cell distribution width (RDW) has been reported to be associated with arterial and venous thrombosis and mortality in several diseases. Here, we analyzed the association between RDW and other red blood cell (RBC) parameters with risk of VTE and mortality in patients with cancer. Methods RBC parameters were measured in 1840 patients with cancers of the brain, breast, lung, stomach, colon, pancreas, prostate, kidney; lymphoma, multiple myeloma and other tumor sites, that were included in the Vienna Cancer and Thrombosis Study (CATS), which is an ongoing prospective, observational cohort study of patients with newly diagnosed or progressive cancer after remission. Primary study outcome is occurrence of symptomatic VTE and secondary outcome is death during a maximum follow-up of 2 years. Results During a median follow-up of 706 days, 131 (7.1%) patients developed VTE and 702 (38.2%) died. High RDW (>16%) was not associated with a higher risk of VTE in the total study cohort; in competing risk analysis accounting for death as competing variable the univariable subhazard ratio (SHR) was 1.34 (95% confidence interval [CI]: 0.80–2.23, p?=?0.269). There was also no significant association between other RBC parameters and risk of VTE. High RDW was associated with an increased risk of mortality in the total study population (hazard ratio [HR, 95% CI]: 1.72 [1.39–2.12], p<0.001), and this association prevailed after adjustment for age, sex, hemoglobin, leukocyte and platelet count (HR [95% CI]: 1.34 [1.06–1.70], p?=?0.016). Conclusions RDW and other RBC parameters were not independently associated with risk of VTE in patients with cancer and might therefore not be of added value for estimating risk of VTE in patients with cancer. We could confirm that high RDW is an independent predictor of poor overall survival in cancer. PMID:25347577

  7. Diagnosis of hyperandrogenism: biochemical criteria.

    PubMed

    Stanczyk, Frank Z

    2006-06-01

    Biochemical derangements in ovarian, adrenal, and peripheral androgen production and metabolism play an important role in underlying causes of hyperandrogenism. Specific diagnostic serum markers such as testosterone (total) and dehydroepiandrosterone sulfate (DHEAS), respectively, may be helpful in the diagnosis of ovarian and adrenal hyperandrogenism, respectively. Validated immunoassays or mass spectrometry assays should be used to quantify testosterone, DHEAS and other principal androgens. Free testosterone measurements, determined by equilibrium dialysis or the calculated method, are advocated for routine evaluation of more subtle forms of hyperandrogenism. The skin, with its pilosebaceous units (PSUs), is an important site of active androgen production. A key regulator in PSUs is 5alpha-reductase, which transforms testosterone or androstenedione to dihydrotestosterone (DHT). DHT in blood is not effective in indicating the presence of hyperandrogenism. However, distal metabolites of DHT have been shown to be good markers of clinical manifestations of hirsutism, acne and alopecia. Assays for these peripheral markers need improvement for routine clinical testing. PMID:16772150

  8. Focused-Ultrasound Disruption of the Blood-Brain Barrier Using Closely-Timed Short Pulses: Influence of Sonication Parameters and Injection Rate

    PubMed Central

    O’Reilly, Meaghan A.; Waspe, Adam C.; Ganguly, Milan; Hynynen, Kullervo

    2011-01-01

    Blood brain barrier disruption (BBBD) shows promise for drug delivery in the brain, however optimal parameters for disruption have yet to be firmly established. Previous work has shown that BBBD can be achieved using bursts comprised of microsecond length pulses at 50% duty cycle in order to eliminate standing waves and variability. The capabilities and limitations of this sort of pulse sequence comprising short bursts were examined. Ultrasound induced BBBD was performed in 28 rats using Definity contrast agent. The spacing between 3 ?s pulses at 1.18 MHz was either 6 ?s, 60 ?s, 300 ?s, 600 ?s during a 10 ms pulse, or 1 s for a single pulse burst. The rate of infusion of the microbubbles was also examined, as well as the burst PRF under infusion conditions. A semi-log relationship between enhancement mean and the number of cycles in a burst was discovered, with a 1 pulse burst (i.e. a 3 ?s burst at 1 Hz) still capable of disrupting the BBB. No increased efficacy or safety benefit over bursts containing more cycles was found, however. Microbubble infusions showed no improvement in T1w enhancement, but did improve consistency. Increased burst PRF combined with infusion improved T1w enhancement but without statistical significance, while a decrease in burst PRF showed a statistically significant decrease in enhancement. PMID:21376455

  9. Environmental Degradation at a Public Park in Southern Brazil as Revealed Through a Genotoxicity Test (MN) on Peripheral Blood Cells from Poecilia vivipara (Teleostei)

    Microsoft Academic Search

    Mônica L. Adam; Rodrigo A. Torres; Graziela Sponchiado; Thalita S. Motta; Cíntia M. R. Oliveira; Marco A. Carvalho-Filho; M. T. S. Correia

    2010-01-01

    The effects of anthropogenic activities on water, environment, and consequently quality of life can be evaluated using genetic,\\u000a biochemical, and microbiological parameters. Regarding genetic parameters, the micronucleus test is a fast, efficient, inexpensive\\u000a method for detecting alterations in genetic material induced by a variety of genotoxic agents. In the present study, blood\\u000a cells from Poecilia vivipara from the Belém River

  10. Quantifying robustness of biochemical network models

    Microsoft Academic Search

    Lan Ma; Pablo A. Iglesias

    2002-01-01

    Background: Robustness of mathematical models of biochemical networks is important for validation purposes and can be used as a means of selecting between different competing models. Tools for quantifying parametric robustness are needed. Results: Two techniques for describing quantitatively the robustness of an oscillatory model were presented and contrasted. Single-parameter bifurcation analysis was used to evaluate the stability robustness of

  11. Biochemical and toxicological studies of aqueous extract of Syzigium aromaticum (L.) Merr. & Perry (Myrtaceae) in rodents.

    PubMed

    Agbaje, E O; Adeneye, A A; Daramola, A O

    2009-01-01

    The effects of long-term administration of boiled aqueous extract of Syzigium aromaticum (SYZ), commonly known as clove (which has been locally employed for treating gastrointestinal tract diseases and also used as food spices), on some biochemical indices, such as body weight, liver functions and blood parameters were studied in adult albino rats of both sexes. Selected doses of 300 and 700 mg kg(-1) were given orally through cannular to groups of animals for a period of 90 days, while the control group received distilled water throughout the duration of study via the same route. Blood samples collected after therapy and assayed for activities of some liver enzymes recorded a significant (p<0.05) and prominent effect on ALP and AST. Measurement of haematological parameters also revealed significant effects (p<0.05; p<0.001) on Hb, RBC (p<0.05), PCV (p<0.001), platelets (p<0.001) and granulocytes (p<0.001). An insignificant reduction was recorded for total WBC. The histopathological study conducted was in consonance with the results of the biochemical investigations that the aqueous extract of SYZ even at moderate doses, significantly affects body organs, their enzymes as well as the various functions. LD(50) for both intraperitoneal and oral routes of SYZ were 263 and 2500 mg kg(-1) respectively. The present work has revealed the toxicity of sub chronic administration of SYZ, which suggests that its prolonged usage must be avoided. PMID:20448849

  12. The Concentrations of Blood Metabolites and the Relations between Blood Parameters, Fatty Acid Composition of Milk and Estimated ME-Balance in Dairy Cows Given Grass Silage ad libitum with Five Different Carbohydrate Supplements

    Microsoft Academic Search

    H. Miettinen; P. Huhtanen

    1989-01-01

    The effects of the carbohydrate composition of the concentrate supplements on certain blood metabolites and the relations between blood metabolites, fatty acid composition of milk and estimated ME-balance were studied in 5 dairy cows given grass silage ad libitum in the 5x5 Latin Square design. Substituting barley with unmolassed sugar beet pulp decreased the average plasma concentration of insulin and

  13. Effects of Acanthopanax senticosus Polysaccharide Supplementation on Growth Performance, Immunity, Blood Parameters and Expression of Pro-inflammatory Cytokines Genes in Challenged Weaned Piglets.

    PubMed

    Han, Jie; Bian, Lianquan; Liu, Xianjun; Zhang, Fei; Zhang, Yiran; Yu, Ning

    2014-07-01

    To investigate the effect of dietary Acanthopanax senticosus polysaccharide (ASPS) on growth performance, immunity, blood parameters and mRNA expression of pro-inflammatory cytokines in immunologically challenged piglets, an experiment employing 2×2 factorial arrangement concerning dietary ASPS treatment (0 or 800 mg/kg) and immunological challenge (lipopolysaccharide [LPS] or saline injection) was conducted with 64 crossbred piglets (weaned at 28 d of age, average initial body weight of 7.25±0.21 kg) assigned to two dietary ASPS treatments with 8 replicates of 4 pigs each. Half of the piglets of per dietary treatment were injected with LPS or saline on d 14. Blood samples were obtained at 3 h after immunological injection on d 14 and piglets were slaughtered to obtain spleen samples on d 21. Dietary ASPS did not affect average daily gain (ADG) (p = 0.634), average daily feed intake (ADFI) (p = 0.655), and gain:feed (p = 0.814) prior to LPS challenge. After LPS challenge, for LPS-challenged pigs those fed ASPS had higher ADG and ADFI than the non-supplemented group (p<0.05), and an interaction between LPS×ASPS was observed on the two indices (p<0.05). Dietary ASPS improved lymphocyte proliferation among saline-injected and LPS-injected pigs (p<0.05). Interaction between LPS×ASPS was also revealed on lymphocyte proliferation (p<0.05). Circulatory concentration of IgG was influenced neither by ASPS (p = 0.803) or LPS (p = 0.692), nor their interaction (p = 0.289). Plasma concentration and spleen mRNA expression of interleukin-1beta (IL-1?), interleukin-6 (IL-6), and tumor necrosis factor (TNF)-? were induced to increase (p<0.05) by LPS challenge, in contrast, these indices were decreased by dietary ASPS (p<0.05), and interactions were found on these cytokines (p<0.05). For LPS-challenged pigs, dietary ASPS also reduced the circulating concentration and spleen mRNA expression of IL-1?, IL-6 as well as TNF-? (p<0.05). The interaction between LPS×ASPS was also observed on the circulating concentration of insulin-like growth factor- I, ?-acid glycoprotein (?-AGP), nonesterified fatty acid, and glucose (p<0.05). The results of this study demonstrate that dietary ASPS can modulate the release of pro-inflammatory cytokines during immunological challenge, which might enable piglets to achieve better growth performance. PMID:25050047

  14. Blood Disorders

    MedlinePLUS

    ... blood cells, white blood cells and platelets. Blood disorders affect one or more parts of the blood ... They can be acute or chronic. Many blood disorders are inherited. Other causes include other diseases, side ...

  15. Blood Transfusions

    MedlinePLUS

    ... their blood . Donors give blood at local blood banks, at community centers during blood drives, or through ... in the world. Many organizations, including community blood banks and the federal government, work hard to ensure ...

  16. Blood Tests

    MedlinePLUS

    ... t have serious reactions to having blood drawn. Laboratory (lab) workers draw the blood and analyze it. They use either whole blood to count blood cells, or they separate the blood cells from the ...

  17. The influence of intravenous laser irradiation of blood on some metabolic and functional parameters in intact rabbits and experimental cerebral ischaemia

    Microsoft Academic Search

    N. Nechipurenko; L. Vasilevskaya; J. Musienko; G. Maslova

    2007-01-01

    It has been studied the intravenous laser irradiation of blood (ILIB) influence with helium-neon laser (HNL) of 630 nm wavelength on some of lipid peroxidation (LPO) and antioxidant system (AOS) findings, aside-base status (ABS) and blood oxygen transport (BOT), state of dermal microhaemodynamics (MGD) in the intact rabbits and after modeling of local ischemia of brain (LIB). Depending on conditions

  18. Postoperative long-term course of peripheral blood immune parameters and immunomodulating effects of keyhole limpet hemocyanin in patients with nonmetastatic renal cell carcinoma.

    PubMed

    Kleinknecht, S; Bichler, K H; Strohmaier, W L

    1992-01-01

    Therapeutic approaches to metastatic renal cell carcinoma (RCC) often focus on the application of immune modulators; the success rates, however, are not satisfactory. Up to the time of this study, no diagnostic tool has been available to select those patients who might profit from immunotherapy. Starting from this point, we have been assessing the immune status of patients suffering from RCC, intending to find markers that would characterize the more favorable prognosis. Our interest is focused not only on the metastatic but also on the nonmetastatic disease, i.e., the disease with the better prognosis. In the present study, we have assessed both the postoperative long-term course of several immune parameters of the peripheral blood and the reactivity of the immune system to immunostimulation with keyhole limpet hemocyanin (KLH) in patients with nonmetastatic RCC. In a prospectively randomized study, the verum group (n = 8) got 1 mg KLH per month up to 1 year while the control group (n = 9) got no immunostimulator after tumor nephrectomy. Both patient groups had stable or even increasing cell counts of lymphocyte subpopulations (T, B, natural killer, T4, T8 cells), and the humoral immunoactivation markers neopterin, beta 2-microglobulin and tumor necrosis factor increased considerably after tumor nephrectomy. An effect of KLH is evident 4-8 months postoperatively: here, the neopterin values in the KLH group are more than twice as high as in the control group. Thus, while patients with metastatic disease had turned out to be immunosuppressed (previous study), in patients with nonmetastatic RCC, both the long-term course indicating postoperative immunostimulation and the reactivity to KLH give evidence of immunocompetence. PMID:1459154

  19. The effects of naturally deoxynivalenol-contaminated oats on the clinical condition, blood parameters, performance and carcass composition of growing pigs

    Microsoft Academic Search

    B. Bergsjø; W. Langseth; I. Nafstad; J. Høgset Jansen; H. J. S. Larsen

    1993-01-01

    A feeding trial with naturally deoxynivalenol (DON)-contaminated oats included in feed mixtures at graded levels was conducted in growing pigs. The DON concentrations were 0, 0.7, 1.7, and 3.5 mg\\/kg of complete feed mixture givenad libitum to different groups. The data recorded were feed consumption, body weight gain, slaughter weight, biochemical and haematological data including serum immunoglobulin A, clinical condition

  20. Biological Biochemical Image Database

    NSDL National Science Digital Library

    The National Institute on Aging -- one of the National Institutes of Health -- provides the Biological Biochemical Image Database, "a searchable database of images of putative biological pathways, macromolecular structures, gene families, and cellular relationships." The database is intended for researchers "working with large sets of genes or proteins using cDNA arrays, functional genomics, or proteomics." The database may be searched by gene name, pathway, cell or tissue type, disease name, biological level, etc. Database users are invited to submit additional diagrams, suggestions, and comments The Web site also includes convenient lists of gene names and keywords, as well as links to biological/ biochemical pathway Web resources.

  1. Modulation of biochemical indices in common carp (Cyprinus carpio L.) under the influence of toxic cyanobacterial biomass in diet.

    PubMed

    Kopp, Radovan; Palíková, Miroslava; Navrátil, Stanislav; Mareš, Jan

    2014-12-01

    Cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. In the present study, we investigated the impact of cyanobacterial biomass on biochemical indices of common carp (Cyprinus carpio L., average weight of 246 ± 73 g) under laboratory conditions. The fish were fed a diet containing cyanobacterial biomass with microcystins in high concentration (0.4 mg/kg of fish weight and day) for 28 days. Statistical evaluation of the influence of the cyanobacterial biomass in food on the biochemical indices of the juvenile carp showed only minor differences. The activity of aspartate aminotransferase value and the urea concentration were significantly reduced compared to control group. The biochemical parameters of fish blood plasma significantly rose during the experiment in the control group as well as in the experimental group. This state was probably influenced by the environmental conditions and the fish diet. A significant rising value was established in calcium creatinine, total protein, phosphorus, lactate, urea and natrium. The present study demonstrates that the oral exposure of toxic cyanobacterial biomass has a minor influence on the biochemical indices of common carp and that the effect of other factors, e.g., nutrition is more visible. PMID:24972534

  2. Blood gases, biochemistry, and hematology of Galapagos green turtles (Chelonia mydas).

    PubMed

    Lewbart, Gregory A; Hirschfeld, Maximilian; Denkinger, Judith; Vasco, Karla; Guevara, Nataly; García, Juan; Muñoz, Juanpablo; Lohmann, Kenneth J

    2014-01-01

    The green turtle, Chelonia mydas, is an endangered marine chelonian with a circum-global distribution. Reference blood parameter intervals have been published for some chelonian species, but baseline hematology, biochemical, and blood gas values are lacking from the Galapagos sea turtles. Analyses were done on blood samples drawn from 28 green turtles captured in two foraging locations on San Cristóbal Island (14 from each site). Of these turtles, 20 were immature and of unknown sex; the other eight were males (five mature, three immature). A portable blood analyzer (iSTAT) was used to obtain near immediate field results for pH, lactate, pO2, pCO2, HCO3-, Hct, Hb, Na, K, iCa, and Glu. Parameter values affected by temperature were corrected in two ways: (1) with standard formulas; and (2) with auto-corrections made by the iSTAT. The two methods yielded clinically equivalent results. Standard laboratory hematology techniques were employed for the red and white blood cell counts and the hematocrit determination, which was also compared to the hematocrit values generated by the iSTAT. Of all blood analytes, only lactate concentrations were positively correlated with body size. All other values showed no significant difference between the two sample locations nor were they correlated with body size or internal temperature. For hematocrit count, the iSTAT blood analyzer yielded results indistinguishable from those obtained with high-speed centrifugation. The values reported in this study provide baseline data that may be useful in comparisons among populations and in detecting changes in health status among Galapagos sea turtles. The findings might also be helpful in future efforts to demonstrate associations between specific biochemical parameters and disease. PMID:24824065

  3. Biochemical upgrading of oils

    DOEpatents

    Premuzic, E.T.; Lin, M.S.

    1999-01-12

    A process for biochemical conversion of heavy crude oils is provided. The process includes contacting heavy crude oils with adapted biocatalysts. The resulting upgraded oil shows, a relative increase in saturated hydrocarbons, emulsions and oxygenates and a decrease in compounds containing organic sulfur, organic nitrogen and trace metals. Adapted microorganisms which have been modified under challenged growth processes are also disclosed. 121 figs.

  4. Nanoparticles as biochemical sensors

    PubMed Central

    El-Ansary, Afaf; Faddah, Layla M

    2010-01-01

    There is little doubt that nanoparticles offer real and new opportunities in many fields, such as biomedicine and materials science. Such particles are small enough to enter almost all areas of the body, including cells and organelles, potentially leading to new approaches in nanomedicine. Sensors for small molecules of biochemical interest are of critical importance. This review is an attempt to trace the use of nanomaterials in biochemical sensor design. The possibility of using nanoparticles functionalized with antibodies as markers for proteins will be elucidated. Moreover, capabilities and applications for nanoparticles based on gold, silver, magnetic, and semiconductor materials (quantum dots), used in optical (absorbance, luminescence, surface enhanced Raman spectroscopy, surface plasmon resonance), electrochemical, and mass-sensitive sensors will be highlighted. The unique ability of nanosensors to improve the analysis of biochemical fluids is discussed either through considering the use of nanoparticles for in vitro molecular diagnosis, or in the biological/biochemical analysis for in vivo interaction with the human body. PMID:24198472

  5. Biochemical upgrading of oils

    DOEpatents

    Premuzic, Eugene T. (East Moriches, NY); Lin, Mow S. (Rocky Point, NY)

    1999-01-12

    A process for biochemical conversion of heavy crude oils is provided. The process includes contacting heavy crude oils with adapted biocatalysts. The resulting upgraded oil shows, a relative increase in saturated hydrocarbons, emulsions and oxygenates and a decrease in compounds containing in organic sulfur, organic nitrogen and trace metals. Adapted microorganisms which have been modified under challenged growth processes are also disclosed.

  6. Blood culture

    MedlinePLUS

    Culture - blood ... A blood sample is needed. The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  7. Blood Transfusion

    MedlinePLUS

    ... from the NHLBI on Twitter. What Is a Blood Transfusion? A blood transfusion is a safe, common ... Very rarely, serious problems develop. Important Information About Blood The heart pumps blood through a network of ...

  8. Blood clotting

    MedlinePLUS Videos and Cool Tools

    ... the external bleeding stops. Clotting factors in the blood cause strands of blood-borne material, called fibrin, to stick together and ... the inside of the wound. Eventually, the cut blood vessel heals, and the blood clot dissolves after ...

  9. What's Blood?

    MedlinePLUS

    ... Let's find out more about each ingredient. Continue Red Blood Cells Red blood cells (also called erythrocytes, say: ih- rith - ... Most of the cells in the blood are red blood cells. They carry around an important chemical ...

  10. Biochemical Effects of Energy Drinks Alone or in Combination with Alcohol in Normal Albino Rats

    PubMed Central

    2014-01-01

    Purpose: To determine the biochemical effects of energy drink alone or in combination with alcohol in normal albino rats. Methods: Twenty male albino rats weighing 160-180g were assigned into groups A-E of four rats per group. Group A and B rats were given low and high doses of ED, respectively, groups C and D were administered low and high doses of EDmA, respectively while group E rats were given distilled water and served as control. The treatment lasted for 30 days after which the animals were killed and their blood collected for laboratory analyses using standard methods. Results: There were no significant differences in body weight, packed cell volume and haemoglobin concentration with either administration of ED or EDmA in comparison to the control. Energy drink alone or EDmA has significant effects on total white blood cell count, plasma potassium, calcium, renal functions, liver enzymes and plasma triglycerides, with EDmA having more effects than ED alone, except for body weight where the energy drink alone has higher effect. Conclusion: Consumption of energy drink alone or in combination with alcohol is associated with significant alterations in some biochemical parameters. Caution should be exercised while consuming either of them. Public health education is urgently needed to correct the wrong impression already formed by the unsuspecting consumers, especially the youths. PMID:24409412

  11. Hematologic and plasma biochemical values of hyacinth macaws (Anodorhynchus hyacinthinus).

    PubMed

    Kolesnikovas, Cristiane K M; Niemeyer, Claudia; Teixeira, Rodrigo H F; Nunes, Adauto L V; Rameh-de-Albuquerque, Luciana C; Sant'Anna, Sávio S; Catão-Dias, José L

    2012-09-01

    The hyacinth macaw (Anodorhyncus hyacinthinus), considered the largest psittacine bird species in the world, is an endangered species, with a remaining population of approximately 6500 birds in the wild. To establish hematologic and plasma biochemical reference ranges and to verify differences related to sex, samples from 29 hyacinth macaws (14 males, 15 females) were obtained from birds apprehended from illegal wildlife trade and subsequently housed at the Sorocaba Zoo, Brazil. No significant differences in hematologic or plasma biochemical values were found between females and males. Compared with published reference values, differences were found in mean concentrations of total red blood cell count, corpuscular volume, corpuscular hemoglobin level, total white blood cell count, aspartate aminotransferase level, creatine kinase concentration, alkaline phosphatase concentration, and phosphorus level. Baseline hematologic and plasma biochemical ranges were established, which may be useful as reference values for clinicians working with this endangered species in captivity or rehabilitation centers. PMID:23156973

  12. Biochemical characterization of microcystin toxicity in rainbow trout ( Oncorhynchus mykiss)

    Microsoft Academic Search

    Francesca Tencalla; Daniel Dietrich

    1997-01-01

    The kinetics and biochemical effects of microcystins in rainbow trout were studied with freeze-dried toxic cells of Microcystis aeruginosa, strain PCC 7806. Following in vivo exposure the changes in liver histology were observed over a 72 hr period and the absorption of microcystins from the gastrointestinal tract into the blood and liver, as well as the inhibition of hepatic protein

  13. Morphological and biochemical changes after intravenous injection of gold nanoparticles

    NASA Astrophysics Data System (ADS)

    Terentyuk, G. S.; Maslyakova, G. N.; Suleymanova, L. V.; Borodulin, V. B.; Dudakova, Yu. S.; Khlebtsov, N. G.; Khlebtsov, B. N.; Akchurin, G. G.; Maksimova, I. L.; Tuchin, V. V.

    2008-12-01

    Advances in nanotechnology applications in medicine, including enhanced cancer therapy cause necessity investigation of nanoparticles toxicity. Herein, we report results encompassing the histological examination of tissues and biochemical tests of blood plasma after intravenous injection of gold nanoparticles. Besides of this, we analyzed passive accumulation of nanoshells in the tumor.

  14. Changes in arterial P O 2 , physiological blood parameters and intracellular antioxidants in free-swimming Atlantic cod ( Gadus morhua ) exposed to varying levels of hyperoxia

    Microsoft Academic Search

    Anders Karlsson; Lene Sørlie Heier; Bjørn Olav Rosseland; Brit Salbu; Anders Kiessling

    2011-01-01

    Free-swimming Atlantic cod (Gadus morhua) were exposed to water oxygen pressures (P\\u000a wO2) ranging from 18.1 to 41.5 kPa and sampled for blood using an indwelling caudal artery cannula. Arterial blood oxygen pressure\\u000a (P\\u000a aO2) increased with increasing P\\u000a wO2, from 12.0 kPa in normoxia (18.1 kPa) to 34.2 kPa in the highest hyperoxic level tested (41.5 kPa). Blood CO2 pressure and plasma bicarbonate concentration

  15. Optical Assay of Erythrocyte Function in Banked Blood

    NASA Astrophysics Data System (ADS)

    Bhaduri, Basanta; Kandel, Mikhail; Brugnara, Carlo; Tangella, Krishna; Popescu, Gabriel

    2014-09-01

    Stored red blood cells undergo numerous biochemical, structural, and functional changes, commonly referred to as storage lesion. How much these changes impede the ability of erythrocytes to perform their function and, as result, impact clinical outcomes in transfusion patients is unknown. In this study we investigate the effect of the storage on the erythrocyte membrane deformability and morphology. Using optical interferometry we imaged red blood cell (RBC) topography with nanometer sensitivity. Our time-lapse imaging quantifies membrane fluctuations at the nanometer scale, which in turn report on cell stiffness. This property directly impacts the cell's ability to transport oxygen in microvasculature. Interestingly, we found that cells which apparently maintain their normal shape (discocyte) throughout the storage period, stiffen progressively with storage time. By contrast, static parameters, such as mean cell hemoglobin content and morphology do not change during the same period. We propose that our method can be used as an effective assay for monitoring erythrocyte functionality during storage time.

  16. Mango Supplementation Improves Blood Glucose in Obese Individuals

    PubMed Central

    Evans, Shirley F; Meister, Maureen; Mahmood, Maryam; Eldoumi, Heba; Peterson, Sandra; Perkins-Veazie, Penelope; Clarke, Stephen L; Payton, Mark; Smith, Brenda J; Lucas, Edralin A

    2014-01-01

    This pilot study examined the effects of freeze-dried mango (Mangifera indica L.) supplementation on anthropometrics, body composition, and biochemical parameters in obese individuals. Twenty obese adults (11 males and 9 females) ages 20- to 50-years old, received 10 g/day of ground freeze-dried mango pulp for 12 weeks. Anthropometrics, biochemical parameters, and body composition were assessed at baseline and final visits of the study. After 12 weeks, mango supplementation significantly reduced blood glucose in both male (?4.45 mg/dL, P = 0.018) and female (?3.56 mg/dL, P = 0.003) participants. In addition, hip circumference was reduced in male (?3.3 cm, P = 0.048) but not in female participants. However, there were no significant changes in body weight or composition in either gender. Our findings indicate that regular consumption of freeze-dried mango by obese individuals does not negatively impact body weight but provides a positive effect on fasting blood glucose. PMID:25210462

  17. Multiplexing oscillatory biochemical signals

    NASA Astrophysics Data System (ADS)

    de Ronde, Wiet; Rein ten Wolde, Pieter

    2014-04-01

    In recent years it has been increasingly recognized that biochemical signals are not necessarily constant in time and that the temporal dynamics of a signal can be the information carrier. Moreover, it is now well established that the protein signaling network of living cells has a bow-tie structure and that components are often shared between different signaling pathways. Here we show by mathematical modeling that living cells can multiplex a constant and an oscillatory signal: they can transmit these two signals simultaneously through a common signaling pathway, and yet respond to them specifically and reliably. We find that information transmission is reduced not only by noise arising from the intrinsic stochasticity of biochemical reactions, but also by crosstalk between the different channels. Yet, under biologically relevant conditions more than 2 bits of information can be transmitted per channel, even when the two signals are transmitted simultaneously. These observations suggest that oscillatory signals are ideal for multiplexing signals.

  18. Clinical and biochemical outcomes of cinacalcet treatment of familial hypocalciuric hypercalcemia: a case series

    PubMed Central

    2011-01-01

    Introduction Familial hypocalciuric hypercalcemia is a rare benign autosomal-dominant genetic disease with high penetrance. In most cases, patients with familial hypocalciuric hypercalcemia experience unspecific physical discomfort or asymptomatic disease. These patients are typically characterized by mild to moderately increased blood ionized calcium and a normal to slightly elevated serum parathyroid hormone. Case presentation Four female patients with familial hypocalciuric hypercalcemia with inactivating mutations in the CaSR gene were included in the treatment study. Three patients were related: two were siblings and one was the daughter of one of these. The ages of the related patients were 51 years, 57 years and 35 years. All three patients were carriers of the same mutation. The fourth patient, unrelated to the others, was 53 years old, and a carrier of a novel and previously unknown mutation leading to familial hypocalciuric hypercalcemia. All four patients were Caucasians of Danish nationality. Biochemically, all patients had elevated blood ionized calcium, serum parathyroid hormone, serum magnesium and total serum calcium, except one, whose serum parathyroid hormone was within the normal range prior to treatment. All patients were treated with cinacalcet in a dosage of 30 mg to 60 mg per day. Conclusion Three months after the initiation of cinacalcet treatment, all our patients experiencing clinical signs of hypercalcemia had improved in self -reported well-being and in biochemical parameters. None of our patients suffered adverse events to cinacalcet treatment. Biochemical markers of calcium homeostasis were improved and remained stable during the observation period of 12 months (two patients), 24 and 36 months, in both the symptomatic and the asymptomatic patients. PMID:22142470

  19. [Biochemical markers of iron status].

    PubMed

    Aimone-Gastin, Isabelle

    2006-11-01

    Traditionally, the standard biochemical markers of iron status are serum iron, transferrin, transferrin saturation, ferritin and, more recently, soluble transferrin receptor. Diagnosis of iron deficiency is usually associated with a low serum ferritin concentration. The diagnosis can be difficult in diseases in which there is an acute-phase response, because ferritin is an acute-phase reactant. In this case, measuring soluble transferrin receptor may be useful because an increased concentration is an indicator of iron deficiency. Iron stores are frequently diminished in patients on dialysis, as a result of increased blood loss and poor iron absorption. Demand for available iron is increased further by the use of erythropoietin and iron deficiency is one of the primary causes for decreased response to recombinant human erythropoeitin therapy (rHuEpo). Prevalence of inflammation in patients on dialysis is estimated to be high (as 50% patients). Because ferritin is an acute-phase reactant, levels may be elevated in cases of inflammation. The aim of recent guidelines is to better assess anaemia and iron stores. Serum ferritin and transferrin saturation are regarded as the most reliable indicators of iron status. A newer alternative laboratory measurement is the soluble transferrin receptor. Some authors suggest that the circulating soluble transferring receptor levels may be useful in monitoring iron status in patients on dialysis if rHuEpo doses are maintained constant. Prospective longitudinal studies are needed to evaluate this hypothesis. PMID:17373277

  20. Portable automatic blood analyzer

    NASA Technical Reports Server (NTRS)

    Coleman, R. L.

    1975-01-01

    Analyzer employs chemical-sensing electrodes for determination of blood, gas, and ion concentrations. It is rugged, easily serviced, and comparatively simple to operate. System can analyze up to eight parameters and can be modified to measure other blood constituents including nonionic species, such as urea, glucose, and oxygen.

  1. Effects of different fibrinogen concentrations on blood loss and coagulation parameters in a pig model of coagulopathy with blunt liver injury

    Microsoft Academic Search

    Oliver Grottke; Till Braunschweig; Dietrich Henzler; Mark Coburn; Rene Tolba; Rolf Rossaint

    2010-01-01

    INTRODUCTION: The early application of fibrinogen could potentially reverse haemodilution-induced coagulopathy, although the impact of varying concentrations of fibrinogen to reverse dilutional coagulopathy has not been studied in vivo. We postulated that fibrinogen concentration is correlated with blood loss in a pig model of coagulopathy with blunt liver injury. METHODS: Coagulopathy was induced in 18 anaesthetized pigs (32 ± 1.6

  2. Clinical biochemical and hormonal profiling in plasma: a promising strategy to predict growth hormone abuse in cattle.

    PubMed

    Doué, Mickael; Dervilly-Pinel, Gaud; Cesbron, Nora; Stefani, Annalisa; Moro, Letizia; Biancotto, Giancarlo; Le Bizec, Bruno

    2015-06-01

    Recombinant bovine somatotrophin (rbST) is widely used in some countries to increase milk production. Since 1994, both marketing and use of this substance have been prohibited within the European Union. In this context, the targeted plasma biochemical and hormonal profiling was assessed as a potential screening strategy to highlight rbST (ab)use in cattle. Twenty-one routinely measured clinical blood parameters, representative of main biological profiles (energetic, proteic, etc.), were measured in the plasma of six lactating cows before and after rbST treatment throughout a 23-day study period. Appropriate multivariate statistical analyses [principal component analysis (PCA) and orthogonal partial least square (OPLS)] enabled discriminating animal samples before and after treatment (days 0 vs. 2 to 9, P?=?2.10(-9)) and highlighted the five most relevant blood parameters in this discrimination. Based on each five-analyte contribution, a simple mathematically weighted equation was suggested to predict the status of samples. A suspicious threshold was proposed, and the model was further tested with the status prediction of the supplementary samples from untreated (n?=?20) and treated cows (n?=?22). The calculated false-positive (10 %) and false-negative (4.5 %) rates were in accordance with the EU requirements for screening methods. Although the model needs to be further validated with additional samples, such targeted plasma biochemical and hormonal profiling already appears as a potential promising screening strategy to highlight rbST (ab)use in cattle. PMID:25716468

  3. CELLS, PROTEINS, AND CERTAIN PHYSICAL-CHEMICAL PROPERTIES OF BROOK TROUT (SALVELINUS FONTINALIS) BLOOD

    EPA Science Inventory

    Laboratory brook trout were used to evaluate, refine, or develop biochemical procedures for the analysis of fish blood. Analytical values were obtained for the following blood properties: total and differential leucocytes and erythrocytes; erythrocyte and plasma proteins (by elec...

  4. Urea, sugar, nonesterified fatty acid and cholesterol content of the blood in prolonged weightlessness

    NASA Technical Reports Server (NTRS)

    Balakhovskiy, I. S.; Orlova, T. A.

    1975-01-01

    Biochemical blood composition studies on astronauts during weightlessness flight simulation tests and during actual space flights showed some disturbances of metabolic processes. Increases in blood sugar, fatty acid and cholesterol, and urea content are noted.

  5. [Effect of fish protein on blood pressure, glycemia and plasma and urinary parameters in spontaneously hypertensive rats (SHR) and diabetic SHR].

    PubMed

    Boukortt, F O; Girard, A; Prost, J; Bouchenak, M; Belleville, J

    2004-01-01

    Fish protein (FP) effect compared to casein (CAS) was studied on blood pressure (BP) evolution and glycemia in part, and on plasma lipid, angiotensine II and aldosterone concentrations and urinary aldosterone on the other hand, in SHR and in SHR with streptozotocin-induced diabetes (SHR-STZ). Two groups of rats (6 SHR and 6 SHR-STZ) were fed for 2 months diet containing 20% CAS or 20% FP. One month before sacrifice, diabetes was induced into one group of rats by a single intraperitonealy injection of streptozotocin (STZ). The results showed that FP diminished blood pressure (-9%) in SHR, compared with CAS. In contrast, FP enhanced significantly blood pressure in SHR-STZ vs SHR (p<0.01). There was no effect on glycemia with fish protein. FP compared to CAS involved a diminution (-41%) and (-17%) of total cholesterol and (-26%) and (-9%) of phospholipids in SHR and SHR-STZ, respectively. Moreover, a decrease of triacylglycerols (-21%) was noted in SHR-STZ with FP vs CAS. In SHR, plasma aldosterone and angiotensine II concentrations were reduced (-62%) and (-64%) and urinary aldosterone amounts were enhanced with FP compared to CAS (p<0.05). In SHR-STZ group, aldosterone value was fivefold lower in plasma and twofold higher in urine with FP compared to CAS. A significant enhancement of urinary aldosterone was noted in SHR-STZ vs SHR whatever diet-consumed. In conclusion, FP has a beneficial effect on blood pressure by modulating the hypertension markers i.e. plasma total cholesterol, angiotensine II and aldosterone, in SHR group, and on total cholesterol and triglycerids in SHR-STZ. FP reduces plasma aldosterone by its enhanced urinary excretion. It is necessary to specify the action mode of FP in order to propose it as nutritional complement in the degenerative diseases such as hypertension and diabetes. PMID:15506056

  6. Blood Clots

    MedlinePLUS

    ... masses of blood. Normally, blood flows freely through veins and arteries . Some blood clotting, or coagulation , is ... Venous clots are those that form in the veins. Venous clots typically form slowly over a period ...

  7. Blood Sugar

    MedlinePLUS

    Blood sugar, or glucose, is the main sugar found in your blood. It comes from the food you eat, and is your body's main source of energy. Your blood carries glucose to all of your body's cells to use ...

  8. Bilirubin - blood

    MedlinePLUS

    ... made by the liver. This article discusses the laboratory test that is done to measure bilirubin in the blood. A small amount of older red blood cells are replaced by new blood cells every day. ...

  9. Blood Components

    MedlinePLUS

    ... of volume) suspended in plasma (~55% of volume). Red cells Red cells, or erythrocytes , carry oxygen from the lungs ... frozen plasma. Transfusable Blood Components Summary Whole Blood Red Blood Cells Platelets Plasma Cryoprecipitated AHF COLOR OF ...

  10. Immunoelectrophoresis - blood

    MedlinePLUS

    IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum immunoglobulin electrophoresis ... A blood sample is needed. For information on how this is done, see: Venipuncture

  11. Comparison of Biochemical Values of Paired Serum and Plasma Samples from American Flamingos ( Phoenicopterus ruber), Indian Runner Ducks ( Anas platyrhynchos), and Hyacinth Macaws ( Anodorhynchus hyacinthinus)

    Microsoft Academic Search

    Keri H. Franco; John P. Hoover; Kay A. Backues; Mark E. Payton

    2010-01-01

    Single blood samples from 8 clinically normal adult Indian runner ducks (Anas platyrhynchos), 8 clinically normal American flamingos (Phoenicopterus ruber), and 3 blood samples from each of 4 clinically normal hyacinth macaws (Anodorhynchus hyacinthinus) were collected to compare the biochemical values of paired plasma samples from heparinized blood with those of serum samples from clotted blood. Small but significant differences

  12. Modeling the growth and dissolution of clots in flowing blood 

    E-print Network

    Mohan, Anand

    2006-10-30

    Multiple interacting mechanisms control the formation and dissolution of clots to maintain blood in a state of delicate balance. In addition to a myriad of biochemical reactions, rheological factors also play a crucial role in modulating...

  13. Short term and dosage influences of palm based medium- and long-chain triacylglycerols on body fat and blood parameters in C57BL/6J mice.

    PubMed

    Lee, Yee-Ying; Tang, Teck-Kim; Ab Karim, Nur Azwani; Alitheen, Noorjahan Banu Mohamed; Lai, Oi-Ming

    2014-01-01

    Structured lipid medium- and long-chain triacylglycerols (MLCT) are claimed to be able to manage obesity. The present study investigated the body fat influence of enzymatically interesterifed palm-based medium- and long-chain triacylglycerols (P-MLCT) on diet-induced obesity (DIO) C57BL/6J mice compared with commercial MLCT oil (C-MLCT) and a control, which was the non enzymatically modified palm kernel and palm oil blend (PKO-PO blend). It also investigated the low fat and high fat effects of P-MLCT. DIO C57BL/6J mice were fed ad libitum with low fat (7%) and high fat (30%) experimental diets for 8 weeks before being sacrificed to obtain blood serum for analysis. From the results, there is a trend that P-MLCT fed mice were found to have the lowest body weight, body weight gain, total fat pad accumulation (perirenal, retroperitoneal, epididymal and mesenteric), total triglyceride levels and efficiency in controlling blood glucose level, compared with C-MLCT and the PKO-PO blend in both low fat and high fat diets. Nevertheless, the PKO-PO blend and P-MLCT caused significantly (P < 0.05) higher total cholesterol levels compared to C-MLCT. P-MLCT present in low fat and high fat dosage were shown to be able to suppress body fat accumulation. This effect is more prominent with the low fat dosage. PMID:24247642

  14. Effects of different levels of hatchery wastes on the performance, carcass and tibia ash and some blood parameters in broiler chicks.

    PubMed

    Mehdipour, M; Shargh, M Shams; Dastar, B; Hassani, S

    2009-09-15

    This experiment was conducted to evaluate the effects of different levels of Hatchery Wastes (HW) on performance, tibia ash, blood calcium and phosphorus concentrations in broiler chickens. Birds were fed a corn-soybean meal diet for 7 days. The experimental treatments included a corn soybean meal diet and 3 other treatments containing 1.5, 3.0 and 4.5% HW. five replicate groups of 15 Ross 308 broiler chicks were allocated to each dietary treatment. Data were analyzed in a completely randomized design. Results indicated that there were no significant differences in weight gains among different dietary treatments. Feed intake in the 4.5% treatment was significantly higher (p < 0.05) and 3% treatment had lower feed intake than the other treatments. The results indicated that feed conversion in the 3% HW treatment was lower in most of the period (p > 0.05). Results of carcass analysis showed no significant differences between treatments. Also, there were no significant differences between blood calcium and phosphorous among treatments. The 4.5 and 3.0% hatchery wastes treatments had the highest tibia ash (p < 0.05). Based on the result of this experiment, the utilization of hatchery wastes as much as 3% can increase tibia strength without having adverse effect on broilers performance. PMID:20384281

  15. Blood phagocyte activity after race training sessions in Thoroughbred and Arabian horses.

    PubMed

    Cywinska, Anna; Szarska, Ewa; Degorski, Andrzej; Guzera, Maciej; Gorecka, Renata; Strzelec, Katarzyna; Kowalik, Sylwester; Schollenberger, Antoni; Winnicka, Anna

    2013-10-01

    Intensive exercise and exertion during competition promote many changes that may result in the impairment of immunity and increased susceptibility to infections. The aim of this study was to evaluate the activity of "the first line of defense": neutrophils and monocytes in racing Thoroughbred and Arabian horses after routine training sessions. Twenty-three (12 Thoroughbred and 11 Arabian) horses were examined. Routine haematological (number of red blood cells - RBC, haemoglobin concentration - HGB, haematocrit - HCT, total number of white blood cells - WBC), biochemical (creatine phosphokinase activity - CPK and total protein concentration - TP) parameters, cortisol concentration as well as phagocytic and oxidative burst activity of neutrophils and monocytes were determined. The values of basic parameters and the activity of phagocytes differed between breeds and distinct patterns of exercise-induced changes were observed. The training sessions did not produce the decrease in phagocyte activity that might lead to the suppression of immunity. PMID:23664016

  16. Hematologic and plasma biochemical values of Spix's macaws (Cyanopsitta spixii).

    PubMed

    Foldenauer, Ulrike; Borjal, Raffy Jim; Deb, Amrita; Arif, Abdi; Taha, Abid Sharif; Watson, Ryan William; Steinmetz, Hanspeter; Bürkle, Marcellus; Hammer, Sven

    2007-12-01

    The Spix's macaw (Cyanopsitta spixii) is considered the world's most endangered parrot, with the last wild bird disappearing in 2001 and only 74 birds in captivity. To establish hematologic and plasma biochemical reference ranges and to look for differences relative to sex, age, and season, we obtained blood samples from 46 captive Spix's macaws (23 male, 23 female) housed in aviaries at the Al Wabra Wildlife Preservation in the State of Qatar. No significant differences in hematologic or plasma biochemical values were found between females and males. Adult and juvenile birds differed in mean concentrations of glucose, total protein, amylase, cholesterol, and phosphorus; in percentages of heterophils and lymphocytes; and in the absolute lymphocyte count. Total protein, cholesterol, and phosphorus concentrations; hematocrit; and heterophil and lymphocyte counts differed significantly by season. Baseline hematologic and plasma biochemical ranges were established, which may be useful as reference values for clinicians working with this highly endangered species. PMID:18351006

  17. Optimizing 18F-FDG PET/CT Imaging of Vessel Wall Inflammation –The Impact of 18F-FDG Circulation Time, Injected Dose, Uptake Parameters, and Fasting Blood Glucose Levels

    PubMed Central

    Bucerius, Jan; Mani, Venkatesh; Moncrieff, Colin; Machac, Josef; Fuster, Valentin; Farkouh, Michael E.; Tawakol, Ahmed; Rudd, James H. F.; Fayad, Zahi A.

    2014-01-01

    Purpose 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) is increasingly used for imaging of vessel wall inflammation. However, limited data is available regarding the impact of methodological variables, i. e. patient’s pre-scan fasting glucose, the FDG circulation time, the injected FDG dose, and of different FDG uptake parameters, in vascular FDG-PET imaging. Methods 195 patients underwent vascular FDG-PET/CT of the aorta and the carotids. Arterial standard uptake values (meanSUVmax) as well as target-to-background-ratios (meanTBRmax) and the FDG blood pool activity in the superior vein cava (SVC) and the jugular veins (JV) were quantified. Vascular FDG uptake classified according to tertiles of patient’s pre-scan fasting glucose levels, the FDG circulation time, and the injected FDG dose was compared using ANOVA. Multivariate regression analyses were performed to identify the potential impact of all variables described on the arterial and blood pool FDG uptake. Results Tertile analyses revealed FDG circulation times of about 2.5 h and prescan glucose levels of less than 7.0 mmol/l showing favorable relations between the arterial and blood pool FDG uptake. FDG circulation times showed negative associations with the aortic meanSUVmax values as well as SVC- and JV FDG blood pool activity but a positive correlation with the aortic- and carotid meanTBRmax values. Pre-scan glucose was negatively associated with aortic- and carotid meanTBRmax and carotid meanSUVmax values, but correlated positively with the SVC blood pool uptake. Injected FDG dose failed to show any significant association with the vascular FDG uptake. Conclusion FDG circulation times and pre-scan blood glucose levels significantly impact FDG uptake within the aortic and carotid wall and may bias the results of image interpretation in patients undergoing vascular FDG-PET/CT. FDG dose injected was less critical. Therefore, circulation times of about 2.5 h and pre-scan glucose levels less than 7.0 mmol/l should be preferred in this setting. PMID:24271038

  18. Biochemical evaluation of an artificial anal sphincter made from shape memory alloys.

    PubMed

    Liu, Hongjian; Luo, Yun; Higa, Masaru; Zhang, Xiumin; Saijo, Yoshifumi; Shiraishi, Yasuyuki; Sekine, Kazumitsu; Yambe, Tomoyuki

    2007-01-01

    Severe anal incontinence is a socially incapacitating disorder and a major unresolved clinical problem that has a considerable negative impact on quality of life. In this study, we developed a new artificial anal sphincter using shape memory alloys (SMAs) in order to improve the quality of life of such patients and evaluated the influence of this sphincter on blood serum chemistry in animal experiments. The artificial anal sphincter was driven by two Ti-Ni SMA actuators sandwiching the intestine and was implanted in three female goats. Blood was collected from the jugular vein on days 1 and 4; at weeks 1 and 2; and at months 1, 2, and 3, postoperatively. Biochemical parameters including total protein, albumin, total bilirubin, aspartate amino-transferase, blood urea nitrogen, creatinine, and C-reactive protein were examined. The time courses of total bilirubin and aspartate amino transferase of the three goats were within the baseline levels after 1 week of implantation and remained normal, demonstrating no liver function complications. The blood urea nitrogen and creatinine levels remained within the normal range, indicating no renal function complications. The total protein and albumin fluctuated within the normal range throughout the duration of this study. In these goats, it was also found that the level of C-reactive protein did not increase and that there was no stricture of the intestine where the artificial sphincter was attached. Our findings indicate that the artificial sphincter SMA demonstrated no adverse influence on blood serum chemistry and exhibited an effective system performance. PMID:18071852

  19. GPU accelerated biochemical network simulation

    PubMed Central

    Zhou, Yanxiang; Liepe, Juliane; Sheng, Xia; Stumpf, Michael P. H.; Barnes, Chris

    2011-01-01

    Motivation: Mathematical modelling is central to systems and synthetic biology. Using simulations to calculate statistics or to explore parameter space is a common means for analysing these models and can be computationally intensive. However, in many cases, the simulations are easily parallelizable. Graphics processing units (GPUs) are capable of efficiently running highly parallel programs and outperform CPUs in terms of raw computing power. Despite their computational advantages, their adoption by the systems biology community is relatively slow, since differences in hardware architecture between GPUs and CPUs complicate the porting of existing code. Results: We present a Python package, cuda-sim, that provides highly parallelized algorithms for the repeated simulation of biochemical network models on NVIDIA CUDA GPUs. Algorithms are implemented for the three popular types of model formalisms: the LSODA algorithm for ODE integration, the Euler–Maruyama algorithm for SDE simulation and the Gillespie algorithm for MJP simulation. No knowledge of GPU computing is required from the user. Models can be specified in SBML format or provided as CUDA code. For running a large number of simulations in parallel, up to 360-fold decrease in simulation runtime is attained when compared to single CPU implementations. Availability: http://cuda-sim.sourceforge.net/ Contact: christopher.barnes@imperial.ac.uk; m.stumpf@imperial.ac.uk Supplementary information: Supplementary data are available at Bioinformatics online. PMID:21224286

  20. Single and combined effects of zinc and cinnamon essential oil in diet on productive performance, egg quality traits, and blood parameters of laying hens reared under cold stress condition.

    PubMed

    Torki, Mehran; Akbari, Mohsen; Kaviani, Keyomars

    2014-11-01

    This study was conducted to evaluate the effects of adding zinc (Zn), cinnamon essential oil (Ci), or their combination in diet on productive performance, egg quality, and blood parameters of laying hens reared under cold stress condition (8.8?±?3 °C). Feed intake (FI), feed conversion ratio (FCR), egg weight (EW), egg production (EP), and egg mass (EM) were evaluated during the 56-day trial period using 120 Lohmann LSL-Lite laying hens. Significant interactions between Ci and Zn on FCR, EW, EP, or EM were observed (P?blood parameters of hens reared under cold stress condition. PMID:25376631

  1. Haematological and serum biochemical responses of chickens to hydric stress.

    PubMed

    Chikumba, N; Swatson, H; Chimonyo, M

    2013-09-01

    Dehydration can be extremely damaging to the performance and welfare of indigenous chickens. The effect of water restriction on haematological and biochemical parameters was compared in Naked Neck (NNK) and Ovambo (OVB) chickens. A total of 54 8-week-old pullets each of NNK and OVB chickens with an initial average weight of 641 ± 10 g/bird were randomly assigned to three water intake treatments with three replications, each having six birds. The water restriction treatments were ad libitum, 70% and 40% of ad libitum intake. Nine experimental pens with a floor space of 3.3 m2 per strain were used. Feed was provided ad libitum. Packed cell volume (PCV), erythrocyte count (RBC), mean corpuscular volume (MCV) and total leucocyte count (WBC), and biochemical parameters (uric acid (UA)), creatinine (CREAT), total protein (TP), albumin (ALB), globulin (GLOB), triglyceride (TGA), total cholesterol (TC), high- (HDLC) and low- (LDLC) density lipoprotein cholesterol and activity of alanine transaminase (ALT), alkaline phosphatase (ALP) and aspartate transaminase (AST) were determined from blood collected after 60 days of water restriction. PCV was higher (P < 0.05) in NNK than OVB chickens offered water ad libitum, but similar in birds offered 70% and 40% of ad libitum. There were no differences in RBC and MCV values between strains, but MCV was higher in birds on 40% than 70% of ad libitum water intake, irrespective of strain. Naked neck chickens had higher (P < 0.05) WBC values than OVB at 40% restriction level, but lower WBC than OVB at 70% water restriction level. UA, CREAT, TGA, TC, LDLC, TP and GLOB increased (P < 0.05) with each increment in water restriction, but the increase in CREAT and TC was more pronounced in OVB than NNK chickens. The opposite was observed for UA. ALT activity indicated that liver function was not affected by water restriction. It was concluded that the two strains can withstand up to 40% of ad libitum water restriction, but NNK tolerated water stress better than OVB chickens. PMID:23764254

  2. Effect of artrofoon and SCENAR therapy on parameters of LPO and antioxidant system of the blood in patients with peritonitis in postoperative period.

    PubMed

    Varakanov, T A; Luspikayan, S Kh; Milyutina, N P; Rozhkov, A V

    2009-09-01

    Administration of artrofoon in combination with SCENAR therapy to patients with localized suppurative peritonitis in the postoperative period considerably reduced plasma MDA level, stabilized ceruloplasmin activity, and increased catalase activity in erythrocytes compared to the corresponding parameters in patients receiving standard treatment in combination with SCENAR therapy. PMID:20396722

  3. Genetic parameters for packed red blood cell volume and daily gain during grazing in Japanese Shorthorn calves facing a natural challenge from Japanese theileriosis

    Microsoft Academic Search

    Michiru Fukasawa; Tadashi Kikuchi; Hisashi Shinohara; Akira Nishida; Toshihiro Yamagishi

    2002-01-01

    Japanese theileriosis, which is caused by the intraerythrocytic protozoan Theileria orientalis sergenti, is one of the most harmful anemic diseases to grazing calves in Japan. In this paper, we estimated genetic and phenotypic parameters of packed cell volume (PCV) and productivity in 435 Japanese Shorthorn calves, and discussed the feasibility of the genetic improvement for higher anemia tolerance. The heritabilities

  4. BIOCHEMICAL STUDIES ON SHOCK

    PubMed Central

    Engel, Frank L.; Harrison, Helen C.; Long, C. N. H.

    1944-01-01

    1. In a series of rats subjected to hemorrhage and shock a high negative correlation was found between the portal and peripheral venous oxygen saturations and the arterial blood pressure on the one hand, and the blood amino nitrogen levels on the other, and a high positive correlation between the portal and the peripheral oxygen saturations and between each of these and the blood pressure. 2. In five cats subjected to hemorrhage and shock the rise in plasma amino nitrogen and the fall in peripheral and portal venous oxygen saturations were confirmed. Further it was shown that the hepatic vein oxygen saturation falls early in shock while the arterial oxygen saturation showed no alteration except terminally, when it may fall also. 3. Ligation of the hepatic artery in rats did not affect the liver's ability to deaminate amino acids. Hemorrhage in a series of hepatic artery ligated rats did not produce any greater rise in the blood amino nitrogen than a similar hemorrhage in normal rats. The hepatic artery probably cannot compensate to any degree for the decrease in portal blood flow in shock. 4. An operation was devised whereby the viscera and portal circulation of the rat were eliminated and the liver maintained only on its arterial circulation. The ability of such a liver to metabolize amino acids was found to be less than either the normal or the hepatic artery ligated liver and to have very little reserve. 5. On complete occlusion of the circulation to the rat liver this organ was found to resist anoxia up to 45 minutes. With further anoxia irreversible damage to this organ's ability to handle amino acids occurred. 6. It is concluded that the blood amino nitrogen rise during shock results from an increased breakdown of protein in the peripheral tissues, the products of which accumulate either because they do not circulate through the liver at a sufficiently rapid rate or because with continued anoxia intrinsic damage may occur to the hepatic parenchyma so that it cannot dispose of amino acids. PMID:19871356

  5. ORIGINAL ARTICLE Blood Transfus DOI 10.2450/2012.0099-11

    E-print Network

    Thomas, David D.

    1 ORIGINAL ARTICLE Blood Transfus DOI 10.2450/2012.0099-11 © SIMTI Servizi Srl Temporal sequence BS et al Blood Transfus DOI 10.2450/2012.0099-11 form of vesicles during cold storage8-12 , however of major biochemical events during Blood Bank storage of packed red blood cells Brad S. Karon1 , Camille M

  6. Immunological and biochemical indicators in turkeys fed diets with a different Methionine content.

    PubMed

    Kubi?ska, M; Tyka?owski, B; Jankowski, J; Koncicki, A

    2014-01-01

    The objective of this study was to determine the effect of different levels of dietary methionine on selected immune parameters of young turkeys. A total of 357 one-day-old female Hybrid Converter turkeys were randomly divided into three groups with low, medium and high level of dietary me- thionine (LM, MM, HM) of seven replicates each. Methionine was added to the basal diet (LM) at 0.16% (MM) and 0.32% (HM). At 17 days of age, seven birds per group were vaccinated against Ornithobacterium rhinotraheale (ORT) infection, with ORNITIN (ABIC) vaccine. At 28 days of age, blood was sampled and the birds were euthanized. The serum concentrations and activity of selected biochemical parameters, total IgY and IgM, and vaccine-induced antibody titers (IgY) against ORT were determined. The percentages of CD4+, CD8+, CD4+/CD8+ T cell subpopulations and IgM+ B cell subpopulation were determined in blood and organs by flow cytometry. Different supplementary levels of methionine had no significant effect on vaccine-induced anti- body titers against ORT or total serum IgM and IgY levels, as well as on the percentages of periph- eral blood T and B cell subsets. Increasing dietary methionine rates decreased the percentage of CD4+ T cell subpopulation, however it has increased the percentage of IgM+ B cell subpopulation in the spleen. Vaccination against ORT resulted in a significant decrease in the percentage of CD4+ T cell subset and an increase in the percentage of CD8+ T cell subset in the spleen. It could be concluded that MM turkeys have developed the most desirable values of immune parameters. PMID:25638983

  7. Hemato-biochemical responses to packing in donkeys administered ascorbic acid during the harmattan season

    PubMed Central

    OLAIFA, Folashade; AYO, Joseph Olusegun; AMBALI, Suleiman Folorunsho; REKWOT, Peter Ibrahim

    2012-01-01

    Experiments were performed to investigate the effect of ascorbic acid (AA) in reducing hemato-biochemical changes in pack donkeys during the cold-dry (harmattan) season. Six experimental donkeys administered orally AA (200 mg/kg) and six control donkeys not administered ascorbic acid were subjected to packing. Blood samples were collected from all donkeys for hematological and biochemical analyses. In the control donkeys, packed cell volume (PCV), erythrocyte count and hemoglobin concentration (Hb) decreased significantly (P<0.05) at the end of packing. In the experimental donkeys, there was no significant difference between the pre- and post-packing values of PCV, erythrocyte count and Hb. In the control donkeys, the neutrophil and neutrophil:lymphocyte ratio increased significantly (P<0.05) post packing, but in the experimental donkeys, the pre- and post-packing values were not significantly different. The eosinophil count increased significantly (P<0.05) in experimental and control donkeys post packing. In conclusion, packing exerted significant adverse effects on the hematological parameters ameliorated by AA administration. AA may modulate neutrophilia and induce a considerable alteration of erythroid markers in donkeys subjected to packing during the harmattan season. PMID:23154452

  8. Haematology and Serum Biochemistry Parameters and Variations in the Eurasian Beaver (Castor fiber)

    PubMed Central

    Girling, Simon J.; Campbell-Palmer, Roisin; Pizzi, Romain; Fraser, Mary A.; Cracknell, Jonathan; Arnemo, Jon; Rosell, Frank

    2015-01-01

    Haematology parameters (N = 24) and serum biochemistry parameters (N = 35) were determined for wild Eurasian beavers (Castor fiber), between 6 months – 12 years old. Of the population tested in this study, N = 18 Eurasian beavers were from Norway and N = 17 originating from Bavaria but now living extensively in a reserve in England. All blood samples were collected from beavers via the ventral tail vein. All beavers were chemically restrained using inhalant isoflurane in 100% oxygen prior to blood sampling. Results were determined for haematological and serum biochemical parameters for the species and were compared between the two different populations with differences in means estimated and significant differences being noted. Standard blood parameters for the Eurasian beaver were determined and their ranges characterised using percentiles. Whilst the majority of blood parameters between the two populations showed no significant variation, haemoglobin, packed cell volume, mean cell haemoglobin and white blood cell counts showed significantly greater values (p<0.01) in the Bavarian origin population than the Norwegian; neutrophil counts, alpha 2 globulins, cholesterol, sodium: potassium ratios and phosphorus levels showed significantly (p<0.05) greater values in Bavarian versus Norwegian; and potassium, bile acids, gamma globulins, urea, creatinine and total calcium values levels showed significantly (p<0.05) greater values in Norwegian versus Bavarian relict populations. No significant differences were noted between male and female beavers or between sexually immature (<3 years old) and sexually mature (?3 years old) beavers in the animals sampled. With Eurasian beaver reintroduction encouraged by legislation throughout Europe, knowledge of baseline blood values for the species and any variations therein is essential when assessing their health and welfare and the success or failure of any reintroduction program. This is the first study to produce base-line blood values and their variations for the Eurasian beaver. PMID:26066344

  9. Quantifying robustness of biochemical network models

    PubMed Central

    2002-01-01

    Background Robustness of mathematical models of biochemical networks is important for validation purposes and can be used as a means of selecting between different competing models. Tools for quantifying parametric robustness are needed. Results Two techniques for describing quantitatively the robustness of an oscillatory model were presented and contrasted. Single-parameter bifurcation analysis was used to evaluate the stability robustness of the limit cycle oscillation as well as the frequency and amplitude of oscillations. A tool from control engineering – the structural singular value (SSV) – was used to quantify robust stability of the limit cycle. Using SSV analysis, we find very poor robustness when the model's parameters are allowed to vary. Conclusion The results show the usefulness of incorporating SSV analysis to single parameter sensitivity analysis to quantify robustness. PMID:12482327

  10. Immediate and short-term effects of exercise on tendon structure: biochemical, biomechanical and imaging responses.

    PubMed

    Tardioli, Alex; Malliaras, Peter; Maffulli, Nicola

    2012-09-01

    Introduction Tendons are metabolically active structures, and their biochemical, biomechanical and structural properties adapt to chronic exercise. However, abnormal adaptations may lead to the development of tendinopathy and pain. Acute and subacute adaptations might contribute to tendon pathology. Sources of data A systematic search of peer-reviewed articles was performed using a wide range of electronic databases. A total of 61 publications were selected. Areas of agreement Exercise induces acute responses in collagen turnover, blood flow, glucose, lactate and other inflammatory products (e.g. prostaglandins and interleukins). Mechanical properties are influenced by activity duration and intensity. Acute bouts of exercise affect tendon structure, with some of the changes resembling those reported in pathological tendons. Areas of controversy Given the variation in study designs, measured parameters and outcomes, it remains debatable how acute exercise influences overall tendon properties. There is discrepancy regarding which investigation modality and settings provide optimal assessment of each parameter. Growing points There is a need for greater homogeneity between study designs, including subject consortium and age, exercise protocols and time frames for parameter assessing. Areas timely for developing research Innovative methods, measuring each parameter simultaneously, would allow a greater understanding of how and when changes occur. This methodology is key to revealing pathological processes and pathways that alter tendon properties according to various activities. Optimal tendon properties differ between activities: more compliant tendons are beneficial for slow stretch shortening cycle (SSC) activities such as countermovement jumps, whereas stiffer tendons are considered beneficial for fast SSC movements such as sprinting. PMID:22279080

  11. Effect of extracorporeal ultraviolet blood irradiation on blood cholesterol level

    NASA Astrophysics Data System (ADS)

    Zalesskaya, G. A.; Laskina, O. V.; Mitkovskaya, N. P.; Kirkovsky, V. V.

    2012-07-01

    We have studied the effect of extracorporeal ultraviolet blood irradiation on cholesterol metabolism in patients with cardiovascular diseases. We have carried out a comprehensive analysis of the spectral characteristics of blood and plasma, gas-exchange and oximetry parameters, and the results of a complete blood count and chemistry panel before and after UV blood irradiation. We have assessed the changes in concentrations of cholesterols (total cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides) in the blood of the patients in response to a five-day course of UV blood irradiation. The changes in the spectral characteristics of blood and plasma, the chemistry panel, the gas composition, and the fractional hemoglobin composition initiated by absorption of UV radiation are used to discuss the molecular mechanisms for the effect of therapeutic doses of UV radiation on blood cholesterols.

  12. Blood Basics

    MedlinePLUS

    ... Series Eight locations, one great program - the only official Highlights of ASH ® . Satellite Symposia ASH offers satellite ... Clinical Studies Published in Blood Search Blood , the official journal of ASH, for the results of the ...

  13. Blood Thinners

    MedlinePLUS

    ... it takes to form a blood clot. Antiplatelet drugs, such as aspirin, prevent blood cells called platelets ... that your healthcare provider knows all of the medicines and supplements you are using.

  14. Reduced mitogenic stimulation of peripheral blood mononuclear cells as a prognostic parameter for the course of breast cancer: a prospective longitudinal study.

    PubMed Central

    Wiltschke, C.; Krainer, M.; Budinsky, A. C.; Berger, A.; Müller, C.; Zeillinger, R.; Speiser, P.; Kubista, E.; Eibl, M.; Zielinski, C. C.

    1995-01-01

    Immunosuppression has been often associated with the course of malignant diseases. In the present study, the proliferation of peripheral blood mononuclear cells (PBMCs) in response to mitogenic stimulation with phytohaemagglutinin (PHA) was assessed prospectively in 90 patients with stage I-III breast cancer. Whereas PHA-induced proliferation of PBMCs derived from patients with breast cancer preoperatively was significantly decreased when compared with data obtained in healthy control individuals (P < 0.001), the degree of the defect in PHA-induced proliferation of PBMCs depended upon the tumour burden as manifested by tumour size and axillary lymph node involvement (P < 0.003 in each case). PHA-induced proliferation of PBMCs dropped significantly in patients who received adjuvant chemotherapy consisting of cyclophosphamide, methotrexate and fluorouracil (CMF) after an observation period of 6 months (P < 0.01), but not in patients under adjuvant treatment with tamoxifen only. After an additional 6 months (i.e. 12 months after surgery), PHA-induced proliferation of PBMCs was similar in patients after adjuvant chemotherapy with CMF and in those receiving continued adjuvant tamoxifen treatment (P > 0.1), but in all patients still significantly decreased as compared with healthy controls (P < 0.001). When data obtained preoperatively and after 12 months were compared, it was found that out of 23 patients whose PBMCs had experienced a drop in their PHA-induced proliferation, 14 (61%) had developed metastatic disease within the subsequent 24 months (i.e. 36 months after surgery). In contrast, out of 59 patients whose PBMCs showed an increase in their PHA-induced proliferation within the first 12 months after surgery, only one (2%) presented with disease progression. We thus conclude that PHA-induced proliferation of PBMCs derived from patients with breast cancer depends upon the tumour load and is a good clinical predictor for the further course of the disease. PMID:7779726

  15. Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens.

    PubMed

    Soria, Mario Alberto; Bonnet, María Agustina; Bueno, Dante Javier

    2015-06-15

    There are few studies about the blood serum of laying hens infected with Salmonella. The differential leukocyte count and blood chemistry values are an important aid in the diagnosis of human diseases, but blood parameters in the avian species are not well known. On the other hand, invasive forms of bacterial gastroenteritis, like Salmonella, often cause intestinal inflammation so this study was undertaken to find a biomarker of Salmonella infection and inflammatory intestinal response in the hematological or serum biochemical parameters in laying hens. Furthermore, we evaluated the association of some farm characteristics with Salmonella infection and fecal leukocytes (FL). A fecal sample with at least one fecal leukocyte per field was considered positive for inflammatory intestinal response. False positive serum reactions for Salmonella infection, by serum plate agglutination (SPA) test, were reduced by heating the sample to 56°C for 30min and then diluting it 5-fold. The range of hematological and biochemical parameter values was very wide, in addition, there was a poor agreement between the SPA and FL results. Comparison of the positive and negative samples in SPA and FL showed that 1.3% and 79.8% of the laying hens were positive and negative in both tests, respectively. Hens with a positive SPA result showed a higher percentage of monocytes than those with a negative SPA result. Hens with a positive FL test had a higher percentage of heterophils, ratio of heterophils to lymphocytes and aspartate aminotransferase values, while the percentage of lymphocytes was significantly lower (P<0.05) than those with a negative FL test. The risk of Salmonella infection increased when the age of laying hens and the number of hens per poultry house was greater than or equal to 18 months old and 10,000 laying hens, compared to less than 18 months old and 10,000 laying hens, respectively. On the other hand, the risk of inflammatory intestinal response was higher in laying hens ?18 months old than in hens <18 months old. Despite the fact that we did not find any specific biomarker of Salmonella infection, this is the first report about the change of Salmonella infection and inflammatory response in hematological/serum biochemical values for laying hens. PMID:25912484

  16. BLOOD FLOW

    NSDL National Science Digital Library

    ARNEL DELA CRUZ

    2012-06-28

    The lesson is design to describe the factors affecting blood flow through the cardiovascular system. The lesson uses the 5E model as an approach for students to become engaged, analytical and inquisitive in learning about the mechanism of blood flow and the importance of this in our body. In addition, the lesson engages the student to test variables that may affect blood flow.

  17. Vomiting blood

    MedlinePLUS

    ... suction to check for blood in the stomach X-rays If you have vomited a lot of blood, you may need emergency treatment. This may include: Administration of oxygen Blood transfusions EGD with application of laser or other modalities to stop the ...

  18. A Course in... Biochemical Engineering.

    ERIC Educational Resources Information Center

    Ng, Terry K-L.; And Others

    1988-01-01

    Describes a chemical engineering course for senior undergraduates and first year graduate students in biochemical engineering. Discusses five experiments used in the course: aseptic techniques, dissolved oxygen measurement, oxygen uptake by yeast, continuous sterilization, and cultivation of microorganisms. (MVL)

  19. Morphological and mineral characteristics of peripheral blood in female polar fox in relation to age.

    PubMed

    Piotrowska, Anna; Szymeczko, Roman; Ozgo, Ma?gorzata; Bogus?awska-Tryk, Monika; Burlikowska, Katarzyna

    2008-01-01

    The aim of the study was to determine the values of selected haematological and biochemical parameters in peripheral blood of female polar fox in relation to the age of the animals. The research involved 50 polar fox females three months after the lactation period (i.e. the non-mating period). Animals were divided into 5 age groups (n = 10), ranging from 1 to 5 years of age. In blood samples the following parameters were determined: RBC, Ht, Hb, WBC, PLT, red blood cell parameters (MCV, MCH, MCHC) and the percentage of respective kinds of white blood cells in the total number of leukocytes. The content of Ca, Pi, Na, K, Cl, Mg, Fe, Cu and Zn as well as the ALP and ACP activity was determined in blood serum. In comparison with one year-old females, in peripheral blood of females from the remaining age groups an increase in RBC, Ht and Hb content was observed as well as a significant (P < 0.05) decrease in WBC level together with a lower number of lymphocytes and an increase in the relative content of granulocytes and monocytes. No distinct relationship between the content of Ca, Na, Cl, Mg, Cu, Zn, the activity of ALP and ACP and the age of the animals was observed. The highest concentration of Pi and K was found in the blood serum of one year-old females. The content of Fe decreased with age and was lowest in 5 year-old animals (P < 0.05). PMID:19055055

  20. Original article Cardio-respiratory, haematological and biochemical

    E-print Network

    Paris-Sud XI, Université de

    period for their cardio-respiratory and metabolic adjustment to strenuous exercise, training and detrainOriginal article Cardio-respiratory, haematological and biochemical parameter adjustments to exercise: effect of a probiotic in horses during training T Art D Votion, K McEntee H Amory, A Linden, R

  1. The jasmonate biochemical pathway.

    PubMed

    Liechti, Robin; Farmer, Edward E

    2003-10-01

    Plants possess an interrelated and interacting family of potent fatty acid-derived regulators--the jasmonates. These compounds, which play roles in both defense and development, are derived from tri-unsaturated fatty acids [alpha-linolenic acid (18:3) or 7Z,10Z,13Z-hexadecatrienoic acid (16:3)]. The lipoxygenase-catalyzed addition of molecular oxygen to alpha-linolenic acid initiates jasmonate synthesis by providing a 13-hydroperoxide substrate for the formation of an unstable allene oxide that is then subject to enzyme-guided cyclization to produce 12-oxo-phytodienoic acid (OPDA). OPDA, a key regulatory lipid in the plant immune system, has several fates, including esterification into plastid lipids or transformation into the 12-carbon co-regulator jasmonic acid (JA). JA, the best-characterized member of the family, regulates both male and female fertility (depending on the plant species), and is an important mediator of defense gene expresssion. JA is itself a substrate for further diverse modifications. Genetic dissection of the pathway is revealing how the different jasmonates modulate different physiological processes. Each new family member that is discovered provides another key to understanding the fine control of gene expression in immune responses, in the initiation and maintenance of long-distance signal transfer in response to wounding, and in the regulation of fertility, among other processes. The Jasmonate Biochemical Pathway provides an overview of the growing jasmonate family, and new members will be included in future versions of the Connections Map. Science Viewpoint R. Liechti, E. E. Farmer, The jasmonate pathway. Science 296, 1649-1650 (2002). [Abstract] [Full Text] PMID:14534325

  2. Histomorphometric and biochemical studies on the liver of rainbow trout (Oncorhynchus mykiss) after exposure to sublethal concentrations of phenol.

    PubMed

    Monfared, Ali Louei; Salati, Amir Parviz

    2013-10-01

    Phenolic compounds are common water pollutants and include a wide variety of organic chemicals. The effects of three sublethal concentrations of phenol on liver histology and some biochemical parameters of Oncorhynchus mykiss after 8 weeks exposure were investigated in this study. A total of 80 rainbow trout (O. mykiss) were randomly divided into four groups (n = 20). Control group was kept in water without any add-on material, while experimental groups were exposed to the concentration of 0.6, 1.2 and 2.4 mg/L of phenol solution. At the end of the study period, the weight of liver, condition factor (CF) and hepatosomatic index (HSI) were measured. Tissue and blood samples were taken separately for histologic evaluation and serum biochemical parameters assay, respectively. In fish treated with phenol, significant reduction in liver weight, CF, HSI ratio, diameter of hepatocytes and hepatocytes nuclear diameter (p < 0.05) was recorded. Total protein and albumin decreased significantly in treated fish in comparison with control (p < 0.05), but levels of cholesterol, glucose, alkaline phosphatase, lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase in fish exposed to different concentrations of phenol showed a significant increase (p < 0.05). These findings showed that phenol could cause its harmful impacts even in very low concentrations. PMID:22740621

  3. Identifying optimal models to represent biochemical systems.

    PubMed

    Apri, Mochamad; de Gee, Maarten; van Mourik, Simon; Molenaar, Jaap

    2014-01-01

    Biochemical systems involving a high number of components with intricate interactions often lead to complex models containing a large number of parameters. Although a large model could describe in detail the mechanisms that underlie the system, its very large size may hinder us in understanding the key elements of the system. Also in terms of parameter identification, large models are often problematic. Therefore, a reduced model may be preferred to represent the system. Yet, in order to efficaciously replace the large model, the reduced model should have the same ability as the large model to produce reliable predictions for a broad set of testable experimental conditions. We present a novel method to extract an "optimal" reduced model from a large model to represent biochemical systems by combining a reduction method and a model discrimination method. The former assures that the reduced model contains only those components that are important to produce the dynamics observed in given experiments, whereas the latter ensures that the reduced model gives a good prediction for any feasible experimental conditions that are relevant to answer questions at hand. These two techniques are applied iteratively. The method reveals the biological core of a model mathematically, indicating the processes that are likely to be responsible for certain behavior. We demonstrate the algorithm on two realistic model examples. We show that in both cases the core is substantially smaller than the full model. PMID:24416170

  4. Selected clinical, biochemical, and electrolyte alterations in anesthetized captive tigers (Panthera tigris) and lions (Panthera leo).

    PubMed

    Reilly, Sabrina; Seddighi, M Reza; Steeil, James C; Sura, Patricia; Whittemore, Jacqueline C; Gompf, Rebecca E; Elliott, Sarah B; Ramsay, Edward C

    2014-06-01

    A prospective study to assess changes in selected plasma biochemistry and electrolyte values, plasma insulin and aldosterone concentrations, and electrocardiography (ECG) was performed on eight female captive tigers (Panthera tigris) and three lions (Panthera leo) undergoing general anesthesia for elective laparoscopic ovariectomy. Each animal was sedated with medetomidine (18-25 microg/kg) and midazolam (0.06-0.1 mg/kg) intramuscularly, and anesthesia was induced with ketamine (1.9-3.5 mg/kg) intramuscularly and maintained with isoflurane. Venous blood samples were collected and analyzed for plasma biochemistry parameters and insulin and aldosterone concentrations. An ECG was recorded at the time of each blood sample collection. Mean plasma potassium, glucose, phosphorus, and aldosterone concentrations increased during anesthesia (P < or = 0.05). One tiger developed hyperkalemia (6.5 mmol/L) 2.5 hr after anesthetic induction. Plasma insulin concentrations were initially below the low end of the domestic cat reference interval (72-583 pmol/L), but mean insulin concentration increased (P < or = 0.05) over time compared with the baseline values. Three tigers and two lions had ECG changes that were representative of myocardial hypoxemia. Based on these results, continuous monitoring of clinical and biochemical alterations during general anesthesia in large nondomestic felids is warranted, and consideration should be given to reversal of medetomidine in these animals should significant changes in electrolytes or ECG occur. PMID:25000694

  5. Original article Ruminal fermentative parameters

    E-print Network

    Paris-Sud XI, Université de

    Original article Ruminal fermentative parameters and blood acido-basic balance changes during (Received 18 April 2003; accepted 10 September 2003) Abstract -- Four ruminal cannulated Texel wethers were used to study the long-term effects of an acidotic diet on ruminal parameters and blood acid

  6. Biochemical and haematological assessment of toxic effects of the leaf ethanol extract of Petroselinum crispum (Mill) Nyman ex A.W. Hill (Parsley) in rats

    PubMed Central

    2013-01-01

    Background Petroselinum crispum, a bright green biennial shrub is widely used traditionally as a food additive and herbal remedies for many ailments. This study therefore aimed to assess the toxic effects of its leaf extract using some biochemical, haematological parameters. Methods The toxic effects were assessed by quantifying liver enzymes such as serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total serum protein and liver weight. Effects on haematological parameters were assessed by analysis of parked cell volume (PCV), red blood cell count (RBC), white blood cells (WBC) and haemoglobin (Hb) concentrations. Histopathological studies were done on the liver and kidneys. Results The extract caused significant increase in serum activity of alanine amino transferase and blood urea nitrogen (BUN) levels at the dose of 1000 mg/kg. Other biochemical and haematological parameters were not affected at lower doses. Conversely, the liver weight was not affected after eight weeks of treatment at the dose levels studied. The organs obtained for pathological study, were structurally unchanged under histopathological evaluation at lower doses but inflammatory and necrotic features were observed at doses???1000 mg/kg. Conclusion The results indicate that the leaf ethanol extract of Petroselinum crispum was hepatotoxic and nephrotoxic at continued oral doses equal to or more than 1000 mg/kg, but no obvious toxicity when used at lower doses. Therefore, there should be caution in its administration to avoid overdosing and known interaction with some medications. In addition, the plant should be kept away from pets and domestic animals and should not be cultivated on soil irrigated with waste water due to their ability to bio-accumulate toxic metals. PMID:23557241

  7. Blood Types

    MedlinePLUS

    ... Templates One-on-One Recruitment Solutions Flyers and Posters Community Outreach Solutions Thank You Note Templates Flyers and Posters Answering Donor Questions Blood Drive Sponsor Stories Sponsor ...

  8. Porphyrins - blood

    MedlinePLUS

    Protoporphyrin levels; Porphyrins - total; Coproporphyrin levels; PROTO test ... amounts in human blood. They are: Coproporphyrin Protoporphyrin (PROTO) Uroporphyrin Protoporphyrin is normally found in the highest ...

  9. Biochemically and histopathologically comparative review of thiamine’s and thiamine pyrophosphate’s oxidative stress effects generated with methotrexate in rat liver

    PubMed Central

    Demiryilmaz, Ismail; Sener, Ebru; Cetin, Nihal; Altuner, Durdu; Suleyman, Bahadir; Albayrak, Fatih; Akcay, Fatih; Suleyman, Halis

    2012-01-01

    Summary Background Oxidative liver injury occurring with methotrexate restricts its use in the desired dose. Therefore, whether or not thiamine and thiamine pyrophosphate, whose antioxidant activity is known, have protective effects on oxidative liver injury generated with methotrexate was comparatively researched in rats using biochemical and histopathological approaches. Material/Methods Thiamine pyrophosphate+methotrexate, thiamine+methotrexate, and methotrexate were injected intraperitoneally in rats for 7 days. After this period, all animals’ livers were excised, killing them with high-dose anesthesia, and histopathologic and biochemical investigations were made. Result Biochemical results demonstrated a significant elevation in level of oxidant parameters such as MDA and MPO, and a reduction in antioxidant parameters such as GSH and SOD in the liver tissue of the methotrexate group. Also, the quantity of 8-OHdG/dG, a DNA injury product, was higher in the methotrexate group with high oxidant levels and low antioxidant levels, and the quantity of 8-OHdG/dG was in the thiamine pyrophosphate group with low oxidant levels and high antioxidant levels. In the thiamine and control groups, the 8-OHdG/dG rate was 1.48±0.35 pmol/L (P>0.05) and 0.55±0.1 pmol/L (P<0.0001). Thiamine pyrophosphate significantly decreased blood AST, ALT and LDH, but methotrexate and thiamine did not decrease the blood levels of AST, ALT and LDH. Histopathologically, although centrilobular necrosis, apoptotic bodies and inflammation were monitored in the methotrexate group, the findings in the thiamine pyrophosphate group were almost the same as in the control group. Conclusions Thiamine pyrophosphate was found to be effective in methotrexate hepatotoxicity, but thiamine was ineffective. PMID:23197226

  10. Pattern selection by dynamical biochemical signals.

    PubMed

    Palau-Ortin, David; Formosa-Jordan, Pau; Sancho, José M; Ibañes, Marta

    2015-03-24

    The development of multicellular organisms involves cells to decide their fate upon the action of biochemical signals. This decision is often spatiotemporally coordinated such that a spatial pattern arises. The dynamics that drive pattern formation usually involve genetic nonlinear interactions and positive feedback loops. These complex dynamics may enable multiple stable patterns for the same conditions. Under these circumstances, pattern formation in a developing tissue involves a selection process: why is a certain pattern formed and not another stable one? Herein we computationally address this issue in the context of the Notch signaling pathway. We characterize a dynamical mechanism for developmental selection of a specific pattern through spatiotemporal changes of the control parameters of the dynamics, in contrast to commonly studied situations in which initial conditions and noise determine which pattern is selected among multiple stable ones. This mechanism can be understood as a path along the parameter space driven by a sequence of biochemical signals. We characterize the selection process for three different scenarios of this dynamical mechanism that can take place during development: the signal either 1) acts in all the cells at the same time, 2) acts only within a cluster of cells, or 3) propagates along the tissue. We found that key elements for pattern selection are the destabilization of the initial pattern, the subsequent exploration of other patterns determined by the spatiotemporal symmetry of the parameter changes, and the speeds of the path compared to the timescales of the pattern formation process itself. Each scenario enables the selection of different types of patterns and creates these elements in distinct ways, resulting in different features. Our approach extends the concept of selection involved in cellular decision-making, usually applied to cell-autonomous decisions, to systems that collectively make decisions through cell-to-cell interactions. PMID:25809268

  11. Speckle-based measurement of the light scattering by red blood cells in vivo

    NASA Astrophysics Data System (ADS)

    Fine, I.; Kaminsky, A.

    2011-03-01

    Optical spectroscopy approach, using non-coherent light sources, has become an important tool for non-invasive analysis in vivo. It is based on the assumption that biochemical characteristics of biological system can be determined through the optical coefficients of blood and tissue particles. Thus, in the framework of this approach, the major concern is to express the obtained optical signals in terms the optical coefficients of the single particle of blood or tissue. However, since the light propagation in tissue is dominated by the multiple-scattering component, a direct measurement of single scattering characteristics turns to be a very difficult task. Practically, only the relative changes of absorption and scattering coefficients are measured. We suggested to adopt the dynamic light scattering (DLS) or speckle technique for the determination of the light scattering coefficients of the red blood cells under stasis conditions in vivo. We assumed that under zero flow conditions the RBC movement is driven mostly by the Brownian motion. It was shown, that under appropriate measurement geometry, the measured optical signal can be decomposed into a few major components. The most dominant components are ascribed to the single backscattering and forward scattering coefficients of the red blood cells. In-vitro and in vivo experimental tests have shown a good correspondence between the theoretically estimated and experientially measured results. The obtained results indicate that the DLS technique can be adopted for the determination of blood particles scattering characteristics in addition to the movement and effective viscosity parameters measurement in vivo.

  12. The role of platelets in blood coagulation during thrombus formation in flow

    E-print Network

    The role of platelets in blood coagulation during thrombus formation in flow A. Tosenberger1,2 , F of aggregating platelets with biochemical reactions in plasma that participate in blood coagulation out the reactants participating in blood coagulation. Platelets covered by the fibrin network cannot

  13. Individual housing influences certain biochemical parameters in the rat

    Microsoft Academic Search

    C. Pérez; J. R. Canal; E. Domínguez; J. E. Campillo; M. Guillén; M. D. Torres

    1997-01-01

    Summary Individual housing has been reported to modify animal behaviour. The present study compares the plasma levels of glucose, triglycerides and total cholesterol, weight, and food and water intake in two groups of female rats. Group A: 10 rats who remained grouped in two cages for 21 days; and Group B: 10 rats housed in two cages for 7 days,

  14. Robustness Analysis of Stochastic Biochemical Systems

    PubMed Central

    ?eska, Milan; Šafránek, David; Dražan, Sven; Brim, Luboš

    2014-01-01

    We propose a new framework for rigorous robustness analysis of stochastic biochemical systems that is based on probabilistic model checking techniques. We adapt the general definition of robustness introduced by Kitano to the class of stochastic systems modelled as continuous time Markov Chains in order to extensively analyse and compare robustness of biological models with uncertain parameters. The framework utilises novel computational methods that enable to effectively evaluate the robustness of models with respect to quantitative temporal properties and parameters such as reaction rate constants and initial conditions. We have applied the framework to gene regulation as an example of a central biological mechanism where intrinsic and extrinsic stochasticity plays crucial role due to low numbers of DNA and RNA molecules. Using our methods we have obtained a comprehensive and precise analysis of stochastic dynamics under parameter uncertainty. Furthermore, we apply our framework to compare several variants of two-component signalling networks from the perspective of robustness with respect to intrinsic noise caused by low populations of signalling components. We have successfully extended previous studies performed on deterministic models (ODE) and showed that stochasticity may significantly affect obtained predictions. Our case studies demonstrate that the framework can provide deeper insight into the role of key parameters in maintaining the system functionality and thus it significantly contributes to formal methods in computational systems biology. PMID:24751941

  15. Blood Facts and Statistics

    MedlinePLUS

    ... About Blood > Blood Facts and Statistics Printable Version Blood Facts and Statistics Facts about blood needs Facts ... about American Red Cross Blood Services Facts about blood needs Every two seconds someone in the U.S. ...

  16. Biology of Blood

    MedlinePLUS

    ... Google+ LinkedIn Merck Manual Home Edition Blood Disorders Biology of Blood Overview of Blood Also of Interest ( ... to Consumer Version DOCTORS: Go to Professional Version Biology of Blood Overview of Blood Components of Blood ...

  17. Biochemical genetic markers in sugarcane

    Microsoft Academic Search

    J. C. Glaszmann; A. Fautret; J. L. Noyer; P. Feldmann; C. Lanaud

    1989-01-01

    Isozyme variation was used to identify biochemical markers of potential utility in sugarcane genetics and breeding. Electrophoretic polymorphism was surveyed for nine enzymes among 39 wild and noble sugarcane clones, belonging to the species most closely related to modern varieties. Up to 114 distinct bands showing presence versus absence type of variation were revealed and used for qualitative characterization of

  18. Solving the Differential Biochemical Jacobian from Metabolomics Covariance Data

    PubMed Central

    Nägele, Thomas; Mair, Andrea; Sun, Xiaoliang; Fragner, Lena; Teige, Markus; Weckwerth, Wolfram

    2014-01-01

    High-throughput molecular analysis has become an integral part in organismal systems biology. In contrast, due to a missing systematic linkage of the data with functional and predictive theoretical models of the underlying metabolic network the understanding of the resulting complex data sets is lacking far behind. Here, we present a biomathematical method addressing this problem by using metabolomics data for the inverse calculation of a biochemical Jacobian matrix, thereby linking computer-based genome-scale metabolic reconstruction and in vivo metabolic dynamics. The incongruity of metabolome coverage by typical metabolite profiling approaches and genome-scale metabolic reconstruction was solved by the design of superpathways to define a metabolic interaction matrix. A differential biochemical Jacobian was calculated using an approach which links this metabolic interaction matrix and the covariance of metabolomics data satisfying a Lyapunov equation. The predictions of the differential Jacobian from real metabolomic data were found to be correct by testing the corresponding enzymatic activities. Moreover it is demonstrated that the predictions of the biochemical Jacobian matrix allow for the design of parameter optimization strategies for ODE-based kinetic models of the system. The presented concept combines dynamic modelling strategies with large-scale steady state profiling approaches without the explicit knowledge of individual kinetic parameters. In summary, the presented strategy allows for the identification of regulatory key processes in the biochemical network directly from metabolomics data and is a fundamental achievement for the functional interpretation of metabolomics data. PMID:24695071

  19. Increased hippocampal blood volume and normal blood flow in schizophrenia.

    PubMed

    Talati, Pratik; Rane, Swati; Skinner, Jack; Gore, John; Heckers, Stephan

    2015-06-30

    Neuroimaging studies have provided compelling evidence for abnormal hippocampal activity in schizophrenia. Most studies made inferences about baseline hippocampal activity using a single hemodynamic parameter (e.g., blood volume or blood flow). Here we studied several hemodynamic measures in the same cohort to test the hypothesis of increased hippocampal activity in schizophrenia. We used dynamic susceptibility contrast- (DSC-) magnetic resonance imaging (MRI) to assess blood volume, blood flow, and mean transit time in the hippocampus of 15 patients with chronic schizophrenia and 15 healthy controls. Left and right hippocampal measurements were combined for absolute measures of cerebral blood volume (CBV), cerebral blood flow (CBF), and mean transit time (MTT). We found significantly increased hippocampal CBV, but normal CBF and MTT, in schizophrenia. The uncoupling of CBV and CBF could be due to several factors, including antipsychotic medication, loss of cerebral perfusion pressure, or angiogenesis. Further studies need to incorporate several complementary imaging modalities to better characterize hippocampal dysfunction in schizophrenia. PMID:25896442

  20. Relationship between the esterase paraoxonase-1 (PON1) and metal concentrations in the whole blood of Inuit in Canada.

    PubMed

    Laird, Brian D; Goncharov, Alexey B; Ayotte, Pierre; Chan, Hing Man

    2015-02-01

    The esterase paraoxonase (PON1), a major component of high-density lipoprotein (HDL), protects against the development of atherosclerosis in humans. Although variation in PON1 activity is primarily governed by PON1 genotype, there is growing evidence that environmental chemicals may also modulate its activity. This cross-sectional study aimed to determine whether environmental exposure to various metals is associated with PON1 activity in Inuit people routinely exposed to mercury (Hg), cadmium (Cd), lead (Pb), and (Se) selenium. PON1 activity and metal concentrations were measured in blood collected from 2172 healthy participants. Sociodemographic, anthropometric and lifestyle variables were also assessed. The associations between PON1 activity and blood metal concentrations, HDL, omega-3 fatty acid blood levels, age, sex, body mass index (BMI), and lifestyle habits (e.g. smoking and alcohol consumption) were explored via multiple linear regression. PON1 activity was positively associated with Se blood concentration (?=0.056, P=0.001) but was negatively associated with Cd blood concentration (?=-0.025, P<0.001). No association was observed between PON1 activity and Hg or Pb blood concentrations. Our results suggest that: PON1 activity is modulated by metal exposure, and Inuit traditional foods may confer health benefit by increasing PON1 activity via higher Se intakes. These findings underline that current environmental metal exposures among Inuit living in the Canadian Arctic are associated with paraoxonase activity, a toxicologically-relevant biochemical parameter. PMID:25260045

  1. Estimating wheat biochemical concentration from field reflectance spectra

    NASA Astrophysics Data System (ADS)

    Liu, Liangyun; Zhang, Bing; Zheng, Lanfen; Tong, Qingxi

    2003-06-01

    The shift of the reflectance red edge parameters is a known phenomenon documenting changes in the biological status and biochemical concentration of plants. In this paper, firstly, the inverted-gaussian (IG) reflectance model was analyzed, and the related reflectance red edge parameters, such as red edge position, ?p, absorption peak position ?o and absorption width ?, were derived. It is absolutely reliable to simulate wheat canopy reflectance red edge by IG model, because the coefficients of determination, R2, between the 403 reflectance red edges (sampled from winter wheat tillering stage to milking satage) and the fitted IG models are large than 0.98. Secondly, red-shifted was found with an early slow increase in the value of ?p, ?o in winter wheat"s tillering, jointing, booting and heading stages; Blue-shifted was found with a late decrease in the value of ?p, ?o in the seed forming and milking stage. Finally, strong correlations were observed between the reflectance red edge parameters and foliar biochemical contents, such as chlorophyll, total nitrogen, sugar, water, from field spectra. The results demonstrated the use of remotely sensed estimates of red edge parameters for estimating biochemical contents, and also indicated the potential of hyperspectral data for precision agriculture.

  2. Clinical application of a novel automatic algorithm for actigraphy-based activity and rest period identification to accurately determine awake and asleep ambulatory blood pressure parameters and cardiovascular risk.

    PubMed

    Crespo, Cristina; Fernández, José R; Aboy, Mateo; Mojón, Artemio

    2013-03-01

    This paper reports the results of a study designed to determine whether there are statistically significant differences between the values of ambulatory blood pressure monitoring (ABPM) parameters obtained using different methods-fixed schedule, diary, and automatic algorithm based on actigraphy-of defining the main activity and rest periods, and to determine the clinical relevance of such differences. We studied 233 patients (98 men/135 women), 61.29?±?.83 yrs of age (mean?±?SD). Statistical methods were used to measure agreement in the diagnosis and classification of subjects within the context of ABPM and cardiovascular disease risk assessment. The results show that there are statistically significant differences both at the group and individual levels. Those at the individual level have clinically significant implications, as they can result in a different classification, and, therefore, different diagnosis and treatment for individual subjects. The use of an automatic algorithm based on actigraphy can lead to better individual treatment by correcting the accuracy problems associated with the fixed schedule on patients whose actual activity/rest routine differs from the fixed schedule assumed, and it also overcomes the limitations and reliability issues associated with the use of diaries. PMID:23130607

  3. A longitudinal study of biochemical variables in women at risk of preeclampsia

    Microsoft Academic Search

    Lucy C. Chappell; Paul T. Seed; Annette Briley; Frank J. Kelly; Beverley J. Hunt; D. Stephen Charnock-Jones; Anthony I. Mallet; Lucilla Poston

    2002-01-01

    Objective: The purpose of this study was to characterize gestational profiles of biochemical markers that are associated with preeclampsia in the blood of pregnant women in whom preeclampsia developed later and to compare these markers with the markers of women who were delivered of small-for-gestational-age infants without preeclampsia and with women who were at low risk for the development of

  4. Physiological and biochemical responses in German Fawn kids subjected to cooling treatments under Mediterranean climate conditions

    Microsoft Academic Search

    Sezen OCAK; Nazan DARCAN; Soner ÇANKAYA; Tamer C. ?NAL

    This study was conducted to determine the effects of different physical modifications (sprinkler and fan) related to heat stress during summer on relevant blood biochemical measures and physiological responses, such as rectal temperature (RT), respiration rate (RR), heart rate (HR), testis temperature (TT), and head temperature (HT), in kids. In total, 32 male German Fawn × Hair kids at 6

  5. Amylase - blood

    MedlinePLUS

    Amylase is an enzyme that helps digest carbohydrates. It is produced in the pancreas and the glands ... saliva. When the pancreas is diseased or inflamed, amylase releases into the blood. A test can be ...

  6. Blood typing

    MedlinePLUS

    ... are slight but may include: Fainting or feeling light-headed Multiple punctures to locate veins Excessive bleeding Hematoma (blood accumulating under the skin) Infection (a slight risk any time the skin is broken)

  7. Donating Blood

    MedlinePLUS

    ... for Parents for Kids for Teens Teens Home Body Mind Sexual Health Food & Fitness Diseases & Conditions Infections Q&A School & Jobs Drugs & Alcohol Staying Safe Recipes En Español ... Image Donating Blood KidsHealth > Teens > Body > Health Basics > ...

  8. Donating Blood

    MedlinePLUS

    ... for Parents for Kids for Teens Teens Home Body Mind Sexual Health Food & Fitness Diseases & Conditions Infections Q&A School & Jobs Drugs & Alcohol Staying Safe Recipes En Español ... Body Image Donating Blood KidsHealth > Teens > School & Jobs > Jobs ...

  9. Blood Cell Palmitoleate-Palmitate Ratio Is an Independent Prognostic Factor for Amyotrophic Lateral Sclerosis

    PubMed Central

    Henriques, Alexandre; Blasco, Hélène; Fleury, Marie-Céline; Corcia, Philippe; Echaniz-Laguna, Andoni; Robelin, Laura; Rudolf, Gabrielle; Lequeu, Thiebault; Bergaentzle, Martine; Gachet, Christian; Pradat, Pierre-François; Marchioni, Eric; Andres, Christian R.; Tranchant, Christine; Gonzalez De Aguilar, Jose-Luis; Loeffler, Jean-Philippe

    2015-01-01

    Growing evidence supports a link between fatty acid metabolism and amyotrophic lateral sclerosis (ALS). Here we determined the fatty acid composition of blood lipids to identify markers of disease progression and survival. We enrolled 117 patients from two clinical centers and 48 of these were age and gender matched with healthy volunteers. We extracted total lipids from serum and blood cells, and separated fatty acid methyl esters by gas chromatography. We measured circulating biochemical parameters indicative of the metabolic status. Association between fatty acid composition and clinical readouts was studied, including ALS functional rating scale-revised (ALSFRS-R), survival, disease duration, site of onset and body mass index. Palmitoleate (16:1) and oleate (18:1) levels, and stearoyl-CoA desaturase indices (16:1/16:0 and 18:1/18:0) significantly increased in blood cells from ALS patients compared to healthy controls. Palmitoleate levels and 16:1/16:0 ratio in blood cells, but not body mass index or leptin concentrations, negatively correlated with ALSFRS-R decline over a six-month period (p<0.05). Multivariate Cox analysis, with age, body mass index, site of onset and ALSFRS-R as covariables, showed that blood cell 16:1/16:0 ratio was an independent prognostic factor for survival (hazard ratio=0.1 per unit of ratio, 95% confidence interval=0.01-0.57, p=0.009). In patients with high 16:1/16:0 ratio, survival at blood collection was extended by 10 months, as compared to patients with low ratio. The 16:1/16:0 index is an easy-to-handle parameter that predicts survival of ALS patients independently of body mass index. It therefore deserves further validation in larger cohorts for being used to assess disease outcome and effects of disease-modifying drugs. PMID:26147510

  10. Whey Protein Improves Exercise Performance and Biochemical Profiles in Trained Mice

    PubMed Central

    CHEN, WEN-CHYUAN; HUANG, WEN-CHING; CHIU, CHIEN-CHAO; CHANG, YU-KAI; HUANG, CHI-CHANG

    2014-01-01

    ABSTRACT Purpose The objective of this study is to verify the beneficial effects of whey protein (WP) supplementation on health promotion and enhance exercise performance in an aerobic-exercise training protocol. Methods In total, 40 male Institute of Cancer Research mice (4 wk old) were divided into four groups (n = 10 per group): sedentary control with vehicle (SC) or WP supplementation (4.1 g·kg?1, SC + WP), and exercise training with vehicle (ET) or WP supplementation (4.1 g·kg?1, ET + WP). Animals in the ET and ET + WP groups underwent swimming endurance training for 6 wk, 5 d·wk?1. Exercise performance was evaluated by forelimb grip strength and exhaustive swimming time as well as by changes in body composition and biochemical parameters at the end of the experiment. Results ET significantly decreased final body and muscle weight and levels of albumin, total protein, blood urea nitrogen, creatinine, total cholesterol, and triacylglycerol. ET significantly increased grip strength; relative weight (%) of liver, heart, and brown adipose tissue (BAT); and levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatine kinase, and total bilirubin. WP supplementation significantly decreased final body, muscle, liver, BAT, and kidney weight and relative weight (%) of muscle, liver, and BAT as well as levels of aspartate aminotransferase, lactate dehydrogenase, creatine kinase, and uric acid. In addition, WP supplementation slightly increased endurance time and significantly increased grip strength and levels of albumin and total protein. Conclusion WP supplementation improved exercise performance, body composition, and biochemical assessments in mice and may be an effective ergogenic aid in aerobic exercise training. PMID:24504433

  11. Atherogenic Dyslipidemia in Children: Evaluation of Clinical, Biochemical and Genetic Aspects

    PubMed Central

    Ceci, Fabrizio; Ferraguti, Giampiero; Ciociola, Ester; Maranghi, Marianna; Gianfagna, Francesco; Iacoviello, Licia; Strom, Roberto; Lucarelli, Marco; Arca, Marcello

    2015-01-01

    The precursors of atherogenic dyslipidemia (AD) are not well defined. Therefore, we investigated 62 non-obese, non-diabetic AD and 221 normolipemic children. Anthropometric parameters, blood pressure and biochemical measures were obtained in index children, their parents and all available siblings. The heritability (h2) of anthropometric and biochemical traits was estimated by SOLAR. Rare and common variants in APOA1 and LPL genes were screened by re-sequencing. Compared to normolipemic, AD children showed increased body mass index, waist circumference, plasma glucose, insulin, ApoB, HOMA-IR, hs-CRP and lower adiponectin (p<0.001 for all). Metabolic syndrome was present in 40% of AD while absent in controls. All traits (except adiponectin and hs-CRP) showed a strong familial aggregation, with plasma glucose having the highest heritability (89%). Overall, 4 LPL loss-of-function mutations were detected (p.Asp9Asn, p.Ser45Asn, p.Asn291Ser, p.Leu365Val) and their cumulative prevalence was higher in AD than in control children (0.073 vs. 0.026; P=0.038). The LPL p.S447* gain-of-function mutation, resulted to be less frequent in AD than in control children (0.064 vs. 0.126; P=0.082). No variant in the APOA1 gene was found. Our data indicate that AD is a rather common dyslipidemia in childhood; it associates with metabolic abnormalities typical of insulin resistant state and shows a strong familial aggregation. LPL variants may contribute to the development of AD phenotype. PMID:25897955

  12. Effects of training on biochemical and functional properties of rodent neonatal heart.

    PubMed

    MacIntosh, A M; Baldwin, K M; Herrick, R E; Mullin, W M

    1985-11-01

    This study was undertaken to determine biochemical and functional (in vivo) adaptations of the rodent neonatal heart in response to a training program of endurance running. Ten day-old rats were progressively trained on a treadmill (final intensity, 21 m/min, 30% grade, 1 h/day) until 75 days of age. The training program induced 14, 57, and 24% increases in relative heart mass, skeletal muscle citrate synthase activity, and whole-body maximal O2 uptake, respectively (P less than 0.05). Cardiac myosin (ATPase) and Ca2+-regulated myofibril ATPase were both reduced by approximately 15% in trained vs. sedentary animals (P less than 0.05). In the majority of trained hearts examined, the myosin isozyme profile reflected an estimated 14 +/- 3% shift toward the V3 or low ATPase isozyme. Left ventricular functional indices during submaximal exercise, derived from a fluid-filled indwelling cannula, indicated that the trained animals maintained similar left ventricular (LV) systolic pressure, LV + the time derivative of pressure, and systemic arterial mean blood pressure compared with their sedentary counterparts. These functional parameters were maintained even though the trained animals performed with lower submaximal exercise heart rate. These findings suggest that maximal exercise capacity can be enhanced in neonatal rats even though the biochemical potential for ATP degradation in the cardiac contractile system is lowered. We speculate that the trend to maintain the myosin isozyme pattern further in the direction of the V3 isozyme in the trained neonatal rat heart may reflect a means to economize cross-bridge cycling while maintaining normal levels of ventricle performance at a given submaximal work load. PMID:2933383

  13. Does haemosporidian infection affect hematological and biochemical profiles of the endangered Black-fronted piping-guan (Aburria jacutinga)?

    PubMed Central

    Ferreira Junior, Francisco Carlos; Andery, Danielle de Assis; Horta, Rodrigo Santos; Peixoto, Renata Barbosa; Lacorte, Gustavo Augusto; Moreira, Patrícia de Abreu; Paes Leme, Fabíola de Oliveira; Melo, Marília Martins; Martins, Nelson Rodrigo da Silva

    2013-01-01

    Infectious diseases can cause deleterious effects on bird species, leading to population decline and extinction. Haemosporidia can be recognized by their negative effects on host fitness, including reproductive success and immune responses. In captivity, outbreaks of haemosporidian infection have been observed in birds in zoos and aviaries. The endemic Brazilian Atlantic rainforest species Aburria jacutinga is one of the most endangered species in the Cracidae family, and wild populations of this species are currently found mainly in conservation areas in only two Brazilian states. In this study, we aimed to evaluate the effects of avian haemosporidia on hematological and biochemical parameters in two captive populations of A. jacutinga. Forty-two animals were assessed, and the haemosporidian prevalence was similar for males and females. The occurrence of haemosporidian infection in captive A. jacutinga observed in this study was similar to results found in other captive and wild birds in Brazil. We found three different lineages of haemosporidia. Two lineages were identified as Plasmodium sp., one of which was previously detected in Europe and Asia, and the other is a new lineage closely related to P. gallinaceum. A new third lineage was identified as Haemoproteus sp. We found no significant differences in hematological and biochemical values between infected and non-infected birds, and the haemosporidian lineage did not seem to have an impact on the clinical and physiological parameters of A. jacutinga. This is the first report on an evaluation of natural haemosporidian infections diagnosed by microscopic and molecular methods in A. jacutinga by hematology, blood biochemistry, and serum protein values. Determining physiological parameters, occurrence and an estimation of the impact of haemosporidia in endangered avian species may contribute to the management of species rehabilitation and conservation. PMID:23638382

  14. Blood characteristics effect on pulse wave velocity.

    PubMed

    Kim, Jong Youn; Yoon, Jihyun; Cho, Minhee; Lee, Byoung-Kwon; Karimi, Ali; Shin, Sehyun

    2013-01-01

    PWV, a surrogate marker for vascular stiffness, can be also expressed by the Bramwell-Hill equation. The effect of blood density to PWV has been ignored, because variation of blood density is assumed to be negligible. In some clinical situation, blood density could be changed, and blood density as a mechanical property of blood flow might affect to PWV. While the elastic property plays an important role in determining the wave propagation in an elastic tube, our assumption is that there might be some relation between blood flow and vascular wall, and that the characteristics of blood flow might influence PWV. This study was objected to investigate the role of mechanical and hemorheologic parameters on PWV in subjects with cardiovascular disease. We have measured and analyzed the PWV, hemorheologic parameters, and other clinical parameters in 814 patients with coronary arterial disease scheduled for coronary angiography. There is no commercial method for measuring whole blood density. So, we defined the density score, which is sum of hemoglobin and total protein. And the hemorheologic parameters were measured within 4 hours after sampling by automated microfluidic hemorheometer. And the effect of all the clinical and hemorheologic parameter on PWV was analyzed by multiple linear regression analysis. Many clinical parameters including age and blood pressure, high shear WBV and ESR as hemorheologic parameters, and density score were correlated well with ba-PWV. However, many clinical variables, high shear WBV and ESR lost the independent significance on multivariable regression analysis. Only age, SBP, and density score were independent variables (p < 0.001). In conclusion, density score as a mechanical property of blood might be suggested as an independent variable influencing PWV in addition to age and blood pressure, but hemorheologic parameters, such as RBC deformability, aggregation, and whole blood viscosity do not affect PWV independently. PMID:23445636

  15. Classic and contemporary approaches to modeling biochemical reactions

    PubMed Central

    Chen, William W.; Niepel, Mario; Sorger, Peter K.

    2010-01-01

    Recent interest in modeling biochemical networks raises questions about the relationship between often complex mathematical models and familiar arithmetic concepts from classical enzymology, and also about connections between modeling and experimental data. This review addresses both topics by familiarizing readers with key concepts (and terminology) in the construction, validation, and application of deterministic biochemical models, with particular emphasis on a simple enzyme-catalyzed reaction. Networks of coupled ordinary differential equations (ODEs) are the natural language for describing enzyme kinetics in a mass action approximation. We illustrate this point by showing how the familiar Briggs-Haldane formulation of Michaelis-Menten kinetics derives from the outer (or quasi-steady-state) solution of a dynamical system of ODEs describing a simple reaction under special conditions. We discuss how parameters in the Michaelis-Menten approximation and in the underlying ODE network can be estimated from experimental data, with a special emphasis on the origins of uncertainty. Finally, we extrapolate from a simple reaction to complex models of multiprotein biochemical networks. The concepts described in this review, hitherto of interest primarily to practitioners, are likely to become important for a much broader community of cellular and molecular biologists attempting to understand the promise and challenges of “systems biology” as applied to biochemical mechanisms. PMID:20810646

  16. Young Blood

    NSDL National Science Digital Library

    American Association for the Advancement of Science (; )

    2005-04-04

    Old muscles heal faster when they're exposed to young blood. That's the conclusion of a recent experiment in mice, led by Stanford University neurologist Thomas Rando. His team found that blood from younger mice stimulated stem cells in the muscles and livers of older mice. Those are the cells that generate new tissue. It's not clear what part of the young blood makes the difference, but Dr. Rando suspects it's a combination of factors. If scientists can solve the mystery, they may be able to develop new ways to help injuries and broken bones heal more quickly. This Science Update looks at the research, which leads to these findings and offers links to other resources for further inquiry. There are also links to Science Netlinks Lesson plans for use at the 6-8 grade level.

  17. Effects of Single Pill-Based Combination Therapy of Amlodipine and Atorvastatin on Within-Visit Blood Pressure Variability and Parameters of Renal and Vascular Function in Hypertensive Patients with Chronic Kidney Disease

    PubMed Central

    Azushima, Kengo; Uneda, Kazushi; Wakui, Hiromichi; Ohsawa, Masato; Kobayashi, Ryu; Dejima, Toru; Kanaoka, Tomohiko; Maeda, Akinobu; Toya, Yoshiyuki; Umemura, Satoshi

    2014-01-01

    Both strict blood pressure (BP) control and improvements in BP profile such as BP variability are important for suppression of renal deterioration and cardiovascular complication in hypertension and chronic kidney disease (CKD). In the present study, we examined the beneficial effects of the single pill-based combination therapy of amlodipine and atorvastatin on achievement of the target BP and clinic BP profile, as well as markers of vascular and renal damages in twenty hypertensive CKD patients. The combination therapy with amlodipine and atorvastatin for 16 weeks significantly decreased clinic BP, and achievement of target BP control was attained in an average of 45% after the combination therapy in spite of the presence of no achievement at baseline. In addition, the combination therapy significantly decreased the within-visit BP variability. With respect to the effects on renal damage markers, combination therapy with amlodipine and atorvastatin for 16 weeks significantly decreased albuminuria (urine albumin-to-creatinine ratio, 1034 ± 1480 versus 733 ± 1218?mg/g-Cr, P < 0.05) without decline in estimated glomerular filtration rate. Concerning parameters of vascular function, the combination therapy significantly improved both brachial-ankle pulse wave velocity (baPWV) and central systolic BP (cSBP) (baPWV, 1903 ± 353 versus 1786 ± 382?cm/s, P < 0.05; cSBP, 148 ± 19 versus 129 ± 23?mmHg, P < 0.01). Collectively, these results suggest that the combination therapy with amlodipine and atorvastatin may exert additional beneficial effects on renal and vascular damages as well as BP profile in addition to BP lowering in hypertension with CKD. PMID:24809050

  18. Effects of single pill-based combination therapy of amlodipine and atorvastatin on within-visit blood pressure variability and parameters of renal and vascular function in hypertensive patients with chronic kidney disease.

    PubMed

    Azushima, Kengo; Uneda, Kazushi; Tamura, Kouichi; Wakui, Hiromichi; Ohsawa, Masato; Kobayashi, Ryu; Dejima, Toru; Kanaoka, Tomohiko; Maeda, Akinobu; Toya, Yoshiyuki; Umemura, Satoshi

    2014-01-01

    Both strict blood pressure (BP) control and improvements in BP profile such as BP variability are important for suppression of renal deterioration and cardiovascular complication in hypertension and chronic kidney disease (CKD). In the present study, we examined the beneficial effects of the single pill-based combination therapy of amlodipine and atorvastatin on achievement of the target BP and clinic BP profile, as well as markers of vascular and renal damages in twenty hypertensive CKD patients. The combination therapy with amlodipine and atorvastatin for 16 weeks significantly decreased clinic BP, and achievement of target BP control was attained in an average of 45% after the combination therapy in spite of the presence of no achievement at baseline. In addition, the combination therapy significantly decreased the within-visit BP variability. With respect to the effects on renal damage markers, combination therapy with amlodipine and atorvastatin for 16 weeks significantly decreased albuminuria (urine albumin-to-creatinine ratio, 1034 ± 1480 versus 733 ± 1218 mg/g-Cr, P < 0.05) without decline in estimated glomerular filtration rate. Concerning parameters of vascular function, the combination therapy significantly improved both brachial-ankle pulse wave velocity (baPWV) and central systolic BP (cSBP) (baPWV, 1903 ± 353 versus 1786 ± 382 cm/s, P < 0.05; cSBP, 148 ± 19 versus 129 ± 23 mmHg, P < 0.01). Collectively, these results suggest that the combination therapy with amlodipine and atorvastatin may exert additional beneficial effects on renal and vascular damages as well as BP profile in addition to BP lowering in hypertension with CKD. PMID:24809050

  19. Understanding Blood Pressure Readings

    MedlinePLUS

    Understanding Blood Pressure Readings Updated:Apr 28,2015 Blood pressure is typically recorded as two numbers, written as a ... blood). What is the AHA recommendation for healthy blood pressure? This chart reflects blood pressure categories defined ...

  20. Blood and Diversity

    MedlinePLUS

    ... Learn About Blood > Blood and Diversity Printable Version Blood and Diversity People come in all different shapes, ... looking for a needle in a haystack. Rare Blood Types Red blood cells carry markers called antigens ...

  1. High Blood Pressure

    MedlinePLUS

    ... of this page please turn Javascript on. High Blood Pressure What Is High Blood Pressure? Click for more information Blood pressure is ... active. So it varies throughout the day. High Blood Pressure (Hypertension) A systolic blood pressure of 140 ...

  2. Blood pressure measurement

    MedlinePLUS

    Diastolic blood pressure; Systolic blood pressure; Blood pressure reading; Measuring blood pressure ... or your health care provider will wrap the blood pressure cuff snugly around your upper arm. The ...

  3. Blood (For Parents)

    MedlinePLUS

    ... a mixture of blood cells and plasma. Continue Red Blood Cells Red blood cells (RBCs, also called ... used in these ways. Back Continue Diseases of Red Blood Cells Most of the time, blood functions ...

  4. Short communication Corynebacterium pseudotuberculosis : biochemical

    E-print Network

    Paris-Sud XI, Université de

    growth for 72 h on TSAS and sheep blood agar plates at 37°C. All strains were also test- ed. For characterization, strains were thawed and cultured twice on Trypticase Soy Agar (Biomérieux, Marcy I'Etoile, France

  5. Blood Vessels

    NSDL National Science Digital Library

    Mrs. Hirschi

    2007-11-20

    Part of the circulatory system is composed of a series of tubes carries the vital elements and the wastes that keep us strong and healthy. Take a look at these amazing vessels and how they work together. Ever cut yourself on the toe? How about the finger? The ear? Ever get a bloody nose? How about a scrape on the knee? If these things have ever happened to you then you already know that blood vessels carry blood to EVERY part of the body. They start out ...

  6. Physiological, biochemical and histometric responses of Nile tilapia (Oreochromis niloticus L.) by dietary organic chromium (chromium picolinate) supplementation

    PubMed Central

    Mehrim, Ahmed I.

    2013-01-01

    Chromium has been recognized as a new and important micro-nutrient, essential for both human and animal nutrition. This study was conducted to evaluate the appropriateness and/or the use of safety level of dietary chromium picolinate (Cr-Pic), and its effects on the physiological responses, the histometric characteristics, and the chemical analysis of dorsal muscles of mono-sex Nile tilapia, Oreochromis niloticus. A total of 420 fingerlings (28.00 ± 0.96 g) were randomly distributed into 21 fiberglass tanks representing seven treatments at a rate of 20 fish m?3. The control fish group (T1) was fed a Cr-Pic free basal diet. Other fish groups were fed the basal diet supplemented with 200 (T2), 400 (T3), 600 (T4), 800 (T5), 1000 (T6) and 1200 ?g Cr-Pic kg?1 diet (T7). Diets were offered to fish at a feeding rate of 3% of life body weight for 12 weeks. Results revealed that blood hematological parameters (hemoglobin, red blood cells, packed cell volume, mean corpuscular hemoglobin concentration, blood platelets, and white blood cells lymphocytes); serum biochemical measurements (total testosterone, high density lipoprotein, total protein, albumin, and globulin); and the dry matter and crude protein of the fish dorsal muscles all have significantly increased (P ? 0.05) in the T3 treatment compared with the other treatments. Meanwhile, no significant differences were found among all treatments with regard to the histometric characteristics. It can be concluded that Cr-Pic at 400 ?g kg?1 diet (T3) seems to be the most appropriate level for O. niloticus fingerlings. PMID:25685497

  7. SyM-BBB: a microfluidic Blood Brain Barrier model.

    PubMed

    Prabhakarpandian, Balabhaskar; Shen, Ming-Che; Nichols, Joseph B; Mills, Ivy R; Sidoryk-Wegrzynowicz, Marta; Aschner, Michael; Pant, Kapil

    2013-03-21

    Current techniques for mimicking the Blood-Brain Barrier (BBB) largely use incubation chambers (Transwell) separated with a filter and matrix coating to represent and to study barrier permeability. These devices have several critical shortcomings: (a) they do not reproduce critical microenvironmental parameters, primarily anatomical size or hemodynamic shear stress, (b) they often do not provide real-time visualization capability, and (c) they require a large amount of consumables. To overcome these limitations, we have developed a microfluidics based Synthetic Microvasculature model of the Blood-Brain Barrier (SyM-BBB). The SyM-BBB platform is comprised of a plastic, disposable and optically clear microfluidic chip with a microcirculation sized two-compartment chamber. The chamber is designed in such a way as to permit the realization of side-by-side apical and basolateral compartments, thereby simplifying fabrication and facilitating integration with standard instrumentation. The individually addressable apical side is seeded with endothelial cells and the basolateral side can support neuronal cells or conditioned media. In the present study, an immortalized Rat Brain Endothelial cell line (RBE4) was cultured in SyM-BBB with a perfusate of Astrocyte Conditioned Media (ACM). Biochemical analysis showed upregulation of tight junction molecules while permeation studies showed an intact BBB. Finally, transporter assay was successfully demonstrated in SyM-BBB indicating a functional model. PMID:23344641

  8. Dietary antioxidants and the biochemical response to oxidant inhalation. II. Influence of dietary selenium on the biochemical effects of ozone exposure in mouse lung

    SciTech Connect

    Elsayed, N.M.; Hacker, A.D.; Kuehn, K.; Mustafa, M.G.; Schrauzer, G.N.

    1983-12-01

    The influence of dietary selenium (Se) on the pulmonary biochemical response to ozone (O/sub 3/) exposure was examined. For 11 weeks, weanling female strain A/St mice were fed a test diet containing Se either at 0 ppm (-Se) or 1 ppm (+Se). Each diet contained 55 ppm vitamin E (vit E). Mice from each dietary group were exposed to 0.8 +/- 0.05 ppm (1568 +/- 98 micrograms/m3) O/sub 3/ continuously for 5 days. After O/sub 3/ exposure, they were killed along with a matched number of unexposed controls, and their lungs were analyzed for various biochemical parameters. The Se contents of lung tissue and whole blood were determined, and the levels were seven- to eightfold higher in +Se mice than in -Se mice, reflecting the Se intake of the animals. In unexposed control mice, Se deficiency caused a decline in glutathione peroxidase (GP) activity relative to +Se group. After O/sub 3/ exposure, the GP activity in the -Se group was associated with a lack of stimulation of glutathione reductase (GR) activity and the pentose phosphate cycle (PPC) as assessed by measuring glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities. In contrast, the +Se group after O/sub 3/ exposure exhibited increases in all four enzyme activities. Other parameters, e.g., lung weight, total lung protein, DNA and nonprotein sulfhydryl contents, and O/sub 2/ consumption, were not affected by dietary Se in the presence or absence of O/sub 3/ exposure. The data indicate that dietary Se alters the GP activity, which in turn influences the GR and PPC activities in the lung evidently through a reduced demand for NADPH. The level of vit E in the lung was found to be twofold higher in the -Se group than in the +Se group, suggesting a compensatory relationship between Se and vit E in the lung. With O3 exposure, both Se and vit E contents further increased in the lungs of each dietary group.

  9. Biochemical and hemodynamic changes in normal subjects during acute and rigorous bed rest and ambulation

    NASA Astrophysics Data System (ADS)

    Zorbas, Yan G.; Kakurin, Vassily J.; Afonin, Victor B.; Yarullin, Vladimir L.

    2002-06-01

    Rigorous bed rest (RBR) induces significant biochemical and circulatory changes. However, little is known about acute rigorous bed rest (ARBR). Measuring biochemical and circulatory variables during ARBR and RBR the aim of this study was to establish the significance of ARBR effect. Studies were done during 3 days of a pre-bed rest (BR) period and during 7 days of ARBR and RBR period. Thirty normal male individuals aged, 24.1±6.3 years were chosen as subjects. They were divided equally into three groups: 10 subjects placed under active control conditions served as unrestricted ambulatory control subjects (UACS), 10 subjects submitted to an acute rigorous bed rest served as acute rigorous bed rested subjects (ARBRS) and 10 subjects submitted to a rigorous bed rest served as rigorous bed rested subjects (RBRS). The UACS were maintained under an average running distance of 9.7 km day -1. For the ARBR effect simulation, ARBRS were submitted abruptly to BR for 7 days. They did not have any prior knowledge of the exact date and time when they would be asked to confine to RBR. For the RBR effect simulation, RBRS were subjected to BR for 7 days on a predetermined date and time known to them right away from the start of the study. Plasma renin activity (PRA), plasma cortisol (PC), plasma aldosterone (PA), plasma and urinary sodium (Na) and potassium (K) levels, heart rate (HR), cardiac output (CO), and arterial blood pressure (ABP) increased significantly, and urinary aldosterone (UA), stroke volume (SV) and plasma volume (PV) decreased significantly ( p<0.05) in ARBRS and RBRS as compared with their pre-BR values and the values in UACS. Electrolyte, hormonal and hemodynamic responses were significantly ( p<0.05) greater and occurred significantly faster ( p<0.05) during ARBR than RBR. Parameters change insignificantly ( p>0.05) in UACS compared with pre-BR control values. It was concluded that, the more abruptly muscular activity is restricted in experimental subjects while they are very active, the greater hemodynamic and biochemical change there is and probably in individuals whose muscular activity is abruptly terminated after an accident or sudden illness.

  10. Blood gases

    MedlinePLUS

    ... the groin Brachial artery in the arm The health care provider may test circulation to the hand before taking a sample ... must remain constant for 20 minutes before the test. Tell your health care provider if you are taking any blood-thinning ...

  11. Blood Types

    E-print Network

    Hacker, Randi; Tsutsui, William

    2007-03-14

    Broadcast Transcript: According to the Japanese, you can tell a lot about a person by their blood type: Type A is the farmer, calm and responsible; Type B is the hunter, independent and creative; Type AB is humanistic, cool and sociable; and Type O...

  12. Biochemical Evaluation of Patients of Alcoholic Liver Disease and Non-alcoholic Liver Disease.

    PubMed

    Torkadi, Prasad P; Apte, I C; Bhute, A K

    2014-01-01

    Alcoholic liver disease (ALD) is due to excessive alcohol intake for long duration. Distinguishing ALD from non-ALD (non-alcoholic steatohepatitis, hepatitis of viral origin) is difficult as patient may deny alcohol abuse. Clinical examination, histology and serology may not differentiate these conditions. Accurate diagnosis is important as management of ALD differs from non-ALD patients. The aim of our study was (1) To evaluate the patients of ALD and non-ALD by biochemical parameters compared to controls, (2) To assess whether these parameters can differentiate ALD from non-ALD. Study was carried out on 50 patients of ALD in group I and 35 patients of NASH (non-alcoholic steatohepatitis) and acute viral hepatitis each in group II. Age matched healthy controls n = 50. Selection criteria-history of alcohol intake (amount and duration), clinical examination, sonography of abdomen, serum alanine transaminase (ALT) and bilirubin levels. Blood samples were analyzed for bilirubin, aspartate transaminase (AST), ALT, alkaline phosphatase (ALP), gamma glutamyl transferase (GGT) by kinetic method. Statistical analysis was done by Student unpaired 't' test. Patients of ALD have raised AST/ALT ratio (De Ritis ratio) (>2), ALP and GGT compared to controls (P < 0.01).There is significant difference in AST/ALT ratio, serum GGT and ALP in ALD group compared to that in NASH and acute viral hepatitis (P < 0.05). This study suggests that De Ritis ratio >2 in ALD patients may be due to alcohol induced hepatic mitochondrial injury and pyridoxine deficiency. High GGT and ALP values may indicate enzyme induction by alcohol and mild cholestasis. Thus ALD patients have severe hepatic damage. De Ritis ratio <1 and normal to mild elevation in GGT level in NASH and acute viral hepatitis suggest mild hepatic injury of non-alcoholic origin. Our study concludes that ALD patients can be differentiated from NASH and acute viral hepatitis with certainty by measuring serum AST/ALT ratio, GGT and ALP. These biochemical parameters may help clinicians to support the diagnosis of ALD and non-ALD. PMID:24478554

  13. Design Principles of Biochemical Oscillators

    PubMed Central

    Novak, Béla; Tyson, John J.

    2009-01-01

    Cellular rhythms are generated by complex interactions among genes, proteins and metabolites. They are used to control every aspect of cell physiology from signaling, motility and development to growth, division and death. By considering specific examples of oscillatory processes, we pick out three general requirements for biochemical oscillations: delayed negative feedback, sufficient ‘nonlinearity’ of the reaction kinetics, and proper balancing of the time-scales of opposing chemical reactions. Positive feedback is one mechanism to delay the negative feedback signal. Biological oscillators can be classified according to the topology of the positive and negative feedback loops in the underlying regulatory mechanism. PMID:18971947

  14. Rheological study on coagulation of blood with special reference to the triggering mechanism of venous thrombus formation

    Microsoft Academic Search

    Makoto Kaibara

    2009-01-01

    A markedly reduced blood flow, an elevation of hematocrit and an increased aggregability of erythrocytes (red blood cells (RBCs)) are risk factors for venous thrombus formation (intravascular blood coagulation). However, these risk factors alone seem to be insufficient to stimulate the coagulation cascade in the absence of a pri- mary triggering mechanism. In this paper, our rheological and biochemical studies

  15. Biochemical diagnosis of occupational exposure to lead toxicity

    SciTech Connect

    Somashekaraiah, B.V.; Venkaiah, B.; Prasad, A.R.K. (Sri Venkateswara Univ., Andhra Pradesh (India))

    1990-02-01

    Lead has been shown to interfere with the biosynthesis of heme in a number of in vitro systems and in experimental animals as well as in human beings. Several steps of the heme biosynthetic chain are subject to the toxic effects of lead. ALA- dehydratase and Ferrochelatase, in particular, are two enzymes which are strongly inhibited by lead, leading to decreased heme synthesis, a constituent of hemoglobin. The inhibition of ALA dehydratase in the red blood cells by lead is generally recognized as the most sensitive index of the individuals exposure to this environmental chemical. Earlier reports show that the determination of blood lead content (Pb-B), zinc protoporphyrin levels and erythrocyte Aminolevulinic acid dehydratase (ALA.D) are widely used as biological indicators for lead toxicity. Hence, the aim of the present study was to screen for occupational exposure to lead in the workers of three different occupations and correlate their blood lead levels with erythrocyte ALA.D and total blood porphyrin content as biochemical indicators of lead exposure.

  16. Three months of regular aerobic exercise in patients with obesity improve systemic subclinical inflammation without major influence on blood pressure and endocrine production of subcutaneous fat.

    PubMed

    Trachta, P; Drápalová, J; Kaválková, P; Toušková, V; Cinkajzlová, A; Lacinová, Z; Matoulek, M; Zelinka, T; Widimský, J; Mráz, M; Haluzík, M

    2014-01-01

    The aim of our study was to explore the effects of regular aerobic exercise on anthropometric, biochemical and hormonal parameters and mRNA expression of selected factors involved in metabolic regulations in subcutaneous adipose tissue of patients with obesity. Fifteen obese women with arterial hypertension underwent a three-month exercise program consisting of 30 min of aerobic exercise 3 times a week. Fifteen healthy lean women with no intervention served as a control group. Obese group underwent anthropometric measurements, blood sampling, subcutaneous adipose tissue (SCAT) biopsy and 24-h blood pressure monitoring at baseline and after three months of exercise, while control group was examined only once. At baseline, obese group had increased SCAT expression of proinflammatory cytokines and adipokines relative to control group. Three months of regular exercise improved anthropometric parameters, decreased CRP, blood glucose and HOMA-IR, while having no significant effect on lipid profile and blood pressure. Gene expressions in SCAT were not affected by physical activity with the exception of increased aquaporin-3 mRNA expression. We conclude that three months of regular exercise decrease systemic subclinical inflammation with only minor influence on the blood pressure and the endocrine function of subcutaneous fat. PMID:24908236

  17. Weighting schemes in metabolic graphs for identifying biochemical routes.

    PubMed

    Ghosh, S; Baloni, P; Vishveshwara, S; Chandra, N

    2014-03-01

    Metabolism forms an integral part of all cells and its study is important to understand the functioning of the system, to understand alterations that occur in disease state and hence for subsequent applications in drug discovery. Reconstruction of genome-scale metabolic graphs from genomics and other molecular or biochemical data is now feasible. Few methods have also been reported for inferring biochemical pathways from these networks. However, given the large scale and complex inter-connections in the networks, the problem of identifying biochemical routes is not trivial and some questions still remain open. In particular, how a given path is altered in perturbed conditions remains a difficult problem, warranting development of improved methods. Here we report a comparison of 6 different weighting schemes to derive node and edge weights for a metabolic graph, weights reflecting various kinetic, thermodynamic parameters as well as abundances inferred from transcriptome data. Using a network of 50 nodes and 107 edges of carbohydrate metabolism, we show that kinetic parameter derived weighting schemes [Formula: see text] fare best. However, these are limited by their extent of availability, highlighting the usefulness of omics data under such conditions. Interestingly, transcriptome derived weights yield paths with best scores, but are inadequate to discriminate the theoretical paths. The method is tested on a system of Escherichia coli stress response. The approach illustrated here is generic in nature and can be used in the analysis for metabolic network from any species and perhaps more importantly for comparing condition-specific networks. PMID:24592291

  18. Biochemical Markers of Neurotoxicity in Wildlife and Human Populations: Considerations for Method Development

    Microsoft Academic Search

    Christopher J. Stamler; Niladri Basu; Hing Man Chan

    2005-01-01

    Disruption of neurochemical parameters in blood and brain tissues can be used as early biomarkers of neurotoxicity in human and wildlife epidemiological studies. To investigate the feasibility of biomarker measurements in field samples obtained from remote locations, tissue storage limits were determined with human blood and mink cortex tissue using efficient and cost-effective microplate assays. Results show that isolated blood

  19. Biochemical abnormalities in Pearson syndrome.

    PubMed

    Crippa, Beatrice Letizia; Leon, Eyby; Calhoun, Amy; Lowichik, Amy; Pasquali, Marzia; Longo, Nicola

    2015-03-01

    Pearson marrow-pancreas syndrome is a multisystem mitochondrial disorder characterized by bone marrow failure and pancreatic insufficiency. Children who survive the severe bone marrow dysfunction in childhood develop Kearns-Sayre syndrome later in life. Here we report on four new cases with this condition and define their biochemical abnormalities. Three out of four patients presented with failure to thrive, with most of them having normal development and head size. All patients had evidence of bone marrow involvement that spontaneously improved in three out of four patients. Unique findings in our patients were acute pancreatitis (one out of four), renal Fanconi syndrome (present in all patients, but symptomatic only in one), and an unusual organic aciduria with 3-hydroxyisobutyric aciduria in one patient. Biochemical analysis indicated low levels of plasma citrulline and arginine, despite low-normal ammonia levels. Regression analysis indicated a significant correlation between each intermediate of the urea cycle and the next, except between ornithine and citrulline. This suggested that the reaction catalyzed by ornithine transcarbamylase (that converts ornithine to citrulline) might not be very efficient in patients with Pearson syndrome. In view of low-normal ammonia levels, we hypothesize that ammonia and carbamylphosphate could be diverted from the urea cycle to the synthesis of nucleotides in patients with Pearson syndrome and possibly other mitochondrial disorders. PMID:25691415

  20. Managing your blood sugar

    MedlinePLUS

    Hyperglycemia - control; Hypoglycemia - control; Diabetes - blood sugar control ... Know how to: Recognize and treat low blood sugar (hypoglycemia) Recognize and treat high blood sugar (hyperglycemia) ...

  1. Adipokines and biochemical changes in Egyptian obese subjects: possible variation with sex and degree of obesity.

    PubMed

    El-Haggar, Sahar Mohamed; Mostafa, Tarek Mohamed

    2015-04-01

    The purpose of this study was firstly to evaluate the adipokines and biochemical changes in obese subjects in relation to different grades of obesity and in relation to gender difference (males versus females) and secondly to evaluate the role of TNF-? in obesity. From January 2013 to February 2014, a total number of 120 non-diabetic subjects of both sexes were recruited and randomly selected from Dr. Abd-Elhamid Elsheikh center for physiotherapy and weight control, El-menofia-Egypt. Those subjects were classified according to their sex into two main groups; the female group and the male group. The female group (60 women) was distributed according to BMI into group 1 (15 lean women), group 2 (15 class I obese women), group 3 (15 class II obese women), and group 4 (15 class III obese women). The male group (60 men) was also distributed according to the BMI into group 1 (15 lean men), group 2 (15 class I obese men), group 3 (15 class II obese men), and group 4 (15 class III obese men). All individuals enrolled in the study were submitted to weight and height measurements with subsequent calculation of body mass index. Fasting blood samples were collected from all participants for quantitative determination of blood glucose, serum lipid, TNF-?, leptin, and adiponectin levels. One-way analysis of variance followed by LSD post hoc test was used for comparison of variables. In obese subjects of both sexes, it was found that circulating leptin and TNF-? levels were significantly high (P<0.05) and positively correlated to BMI. In contrast to leptin, adiponectin concentrations were significantly low (P<0.05) and inversely correlated to BMI. Regarding gender difference, although serum leptin and adiponectin levels were higher in women than men, men showed higher atherogenic parameters. We conclude that leptin, TNF-?, and adiponectin were related to both BMI and grades of obesity. Furthermore, TNF-? may play a role in obesity. PMID:25142747

  2. Clinical features of biochemical cholestasis in patients with recurrent hepatitis C after living-donor liver transplantation.

    PubMed

    Ueda, Y; Takada, Y; Marusawa, H; Haga, H; Sato, T; Tanaka, Y; Egawa, H; Uemoto, S; Chiba, T

    2010-07-01

    Recurrent hepatitis C after liver transplantation (HepC-LT) progresses faster than hepatitis C in non-transplant settings. Cholestasis has been suggested to be one characteristic of HepC-LT related to the rapid progression. We investigated the clinical features of biochemical cholestasis, which we defined as high serum concentrations of alkaline phosphatase and gamma-glutamyl transpeptidase, in patients with recurrent hepatitis C after living-donor liver transplantation. Eighty patients were diagnosed with post-transplant recurrent hepatitis C after exclusion of other aetiologies of cholestasis by liver biopsy and imaging. The clinical features of biochemical cholestasis in the patients with HepC-LT, including histological changes, the efficacy of interferon therapy and helper T-cell (Th) subsets in the peripheral blood, were analysed. Fifty-five of the 80 patients with HepC-LT (69%) had evidence of biochemical cholestasis. Progression of liver fibrosis to stage F3 or F4 was significantly accelerated in patients with biochemical cholestasis compared with patients without cholestasis. The biochemical cholestasis in patients with HepC-LT improved after interferon therapy in 22 of 39 patients (56%) who showed a virological response to the therapy, suggesting that hepatitis C virus (HCV) caused the biochemical cholestasis in these patients. Patients with biochemical cholestasis who had a biochemical response to interferon therapy showed an increased Th1 responses in peripheral blood. In conclusion, biochemical cholestasis is the characteristic feature of HepC-LT and is related to progression of liver fibrosis. An increased Th1 response is associated with cholestasis caused by HCV after liver transplantation. PMID:19804502

  3. Changes in Serum Biochemical Factors Associated with Opium Addiction after Addiction Desertion

    PubMed Central

    Afarinesh, Mohammad Reza; Haghpanah, Tahereh; Divsalar, Kouros; Dehyadegary, Elham; Shaikh-Aleslami, Azar; Mahmoodi, Majid

    2014-01-01

    Background The long time use of opium has some effects on serums biochemical factors, the determination of this variation is a new approach in understanding off addiction and relive of drug abuser health. Hence in this study, these indicators in person who were withdrawing of opium have been studied. Methods In this cross-sectional study bloods biochemical factors such as fasting blood sugar (FBS), sodium (Na), calcium (Ca), uric acid (UA), blood urea nitrogen (BUN), creatinine, cholesterol, total protein, and fibrinogen in three groups serum were studied: (1) Who had been permanent opium users more than 2 years (case). (2) Dependent person who has taken one month addiction withdrawal course (control). (3) A healthy group that had been demographically similar to the other groups. Findings According to these study findings, FBS serum level in the case group is lower than control group. Serum level of Na, creatinine, and blood triglyceride (TG) in case study are higher than group control. Concentration of potassium, Ca, UA, BUN, cholesterol, total serum protein, fibrinogen, and thrombin time in case study and group control showed no significant difference. Also, in withdrawing case serum level of Na, Ca, UA, BUN, creatinine, and TG significantly increase and thrombin time decrease. Conclusion According to this study not only the longtime use of opium but also opium with drawerin opium dependent people can change their serum biochemical factors. So recognition, treatment, and prevention of this change could be a new step in improving of health and condition of patients. PMID:25984281

  4. [Determination of maximum permissible concentrations of industrial toxicants using the integral biochemical index].

    PubMed

    Sidorov, V S; Nemova, N N; Vysotskaia, R U; Feklov, Iu A

    2002-01-01

    Effects of patented mixtures of substances, used as drilling fluids in petroleum industry, on the activity of enzymes (cathepsin D, EC 3.4.23.5; catalase, EC 1.11.1.6; and DNase, EC 3.1.4.6) and the content of analytes (malondialdehyde, fatty acids, free and collagen-associated hydroxyproline, bile acids, and total protein) in liver, gills, muscles, gonads, and bile have been studied under aquarium conditions in mature river flounder and one-year-old salmon for the purpose of determining maximum permissible concentrations. Measuring 25-30 independent biochemical parameters per organ is sufficient for establishing a direct relationship between the concentration of an industrial toxicant and the integral biochemical index, a new characteristic defined as the ratio of the number of biochemical parameters significantly deviating from control values to the total number of the parameters measured. PMID:12068591

  5. Lead levels - blood

    MedlinePLUS

    Blood lead levels ... A blood sample is needed. Most of the time blood is typically drawn from a vein located on the ... may be used to puncture the skin. The blood collects in a small glass tube called a ...

  6. High Blood Pressure (Hypertension)

    MedlinePLUS

    ... Page Text Size: A A A Listen High Blood Pressure (Hypertension) Nearly 1 in 3 American adults ... if your doctor prescribes it, medicine. What Is Blood Pressure? Blood pressure is the force of blood ...

  7. High Blood Pressure

    MedlinePLUS

    ... during light physical activity or exercise. What Is Blood Pressure? Blood pressure is the force of blood ... stated as 120/80. Do You Have High Blood Pressure? One reason to have regular visits to ...

  8. Blood Transfusion (For Parents)

    MedlinePLUS

    ... in the world. Many organizations, including community blood banks and the federal government, work hard to ensure ... blood is then sent to the hospital's blood bank lab, where technicians test it for blood type. ...

  9. What Is Blood?

    MedlinePLUS

    ... transports water and nutrients to your body’s tissues. Red blood cells carry oxygen. Red blood cells are ... the presence or absence of certain substances on red blood cells. Blood types are inherited. Another important ...

  10. Special Blood Donation Procedures

    MedlinePLUS

    ... whole blood, which takes about 10 minutes. Double red blood cell donation In the so-called double ... cells), and thrombocythemia (an excess of platelets). In red blood cell exchange, red blood cells are removed ...

  11. Biochemical transformation of solid carbonaceous material

    DOEpatents

    Lin, Mow S. (Rocky Point, NY); Premuzic, Eugene T. (East Moriches, NY)

    2001-09-25

    A method of biochemically transforming macromolecular compounds found in solid carbonaceous materials, such as coal is provided. The preparation of new microorganisms, metabolically weaned through challenge growth processes to biochemically transform solid carbonaceous materials at extreme temperatures, pressures, pH, salt and toxic metal concentrations is also disclosed.

  12. Toxic Effects of Silver Nanoparticles on Liver and Some Hematological Parameters in Male and Female Mice (Mus musculus).

    PubMed

    Heydrnejad, M Saeed; Samani, Roya Jafarzadeh; Aghaeivanda, Simin

    2015-06-01

    This research was carried out to evaluate toxic effects of nanosilver (Ag-NPs) on liver function and some blood parameters of male and female mice Mus musculus. A group of 54 BALB/c mice was randomly divided into three groups (each with two replications): Ag-NP (2) and control (1), each with nine mice. The experiment lasted for 14 days. In the treatment groups, two different doses of 20 and 50 ppm of Ag-NP solution were administered orally, while in the untreated (control) group, no Ag-NP solution but distilled water was used. At the end of the experiment, the serum was obtained by centrifugation of the whole blood at 3000 rpm for 15 min. The biochemical levels including alanine aminotransferase (ALT), aspartate amino transferase (AST), and blood cells were assayed by an automatic biochemical analyzer. Also, liver biopsy was performed and samples were stained using hematoxylin and eosin (H&E) staining. The values of red blood cells (RBC), hemoglobin (Hb), and hematocrit (Hct) did not vary significantly in the control and Ag-NP-treated animals. There were significant changes in the treatment and control groups in the levels of liver enzymes so that at both doses, there were significantly elevated levels of ALT and AST in mice treated with Ag-NPs compared with the control (p?blood chemistry and hepatotoxicity as indicated by increased serum activity levels of both AST and ALT and histological damages to the liver with no significant changes between male and female mice. PMID:25637567

  13. Effects of mulberry leaf extract rich in 1-deoxynojirimycin on blood lipid profiles in humans.

    PubMed

    Kojima, Yoshihiro; Kimura, Toshiyuki; Nakagawa, Kiyotaka; Asai, Akira; Hasumi, Keiji; Oikawa, Shinichi; Miyazawa, Teruo

    2010-09-01

    Mulberry leaves are rich in 1-deoxynojirimycin (DNJ), an inhibitor of ?-glucosidase. We previously showed that DNJ-rich mulberry leaf extract suppressed elevation of postprandial blood glucose in humans. The objective of this study was to evaluate the effects of DNJ-rich mulberry leaf extract on plasma lipid profiles in humans. An open-label, single-group study was conducted in 10 subjects with initial serum triglyceride (TG) level ?200 mg/dl. Subjects ingested capsules containing DNJ-rich mulberry leaf extract at 12 mg three times daily before meals for 12 weeks. Our findings showed a modest decrease in serum TG level and beneficial changes in the lipoprotein profile following 12-week administration of DNJ-rich mulberry leaf extract. No significant changes in hematological or biochemical parameters were observed during the study period; no adverse events associated with DNJ-rich mulberry leaf extract occurred. PMID:20838571

  14. Study of Eight Blood Protein Polymorphic Systems in Arabian Horses from Turkey

    Microsoft Academic Search

    M. Uzun; A. Karkhan; A. Kopar

    2001-01-01

    Analysis of the blood protein system was used to study the genetic composition of Arabian horses. Biochemical markers of eight polymorphic loci (Tf, Al, As, AlB, Gc, Hb, PGD, and PGM) were electrophoretically identified in blood samples. A total of 43 phenotypes were identified for these polymorphic systems. The Tf, Hb, and Esloci appeared to be more polymorphic than the

  15. Low blood selenium levels in alcoholics with and without advanced liver disease

    Microsoft Academic Search

    Brad Dworkin; William S. Rosenthal; Rita H. Jankowski; Gary G. Gordon; Daulat Haldea

    1985-01-01

    Selenium deficiency has been implicated as a cause of hepatic injury, possibly from accentuated lipoperoxidation due to decreased activity of the selenoenzyme, glutathione peroxidase. Because of possible clinical and biochemical links between selenium and alcohol, we performed nutritional assessment and assayed red blood cell, plasma, and whole blood selenium by spectrofluorometry in 27 normals (group I), 30 asymptomatic alcoholics on

  16. Blood gas monitoring using microflow colorimetry

    NASA Astrophysics Data System (ADS)

    Cooney, Christopher; Towe, Bruce C.

    1999-05-01

    Optical monitoring of blood gas concentrations is achieved using sub microliter quantities of a colorimetric indicator continuously perfused through microdialysis hollow fiber membranes in blood contact. The hollow fiber membranes, selected based on their permeabilities in blood, are silicone for both oxygen and carbon dioxide sensing, and cuproammonium rayon for pH sensing. A sweep fluid passed through the lumen of these fibers undergoes a diffusive equilibrium with the blood and then is continuously mixed with an indicator. A 450 nm gallium nitride LED is used to excite an oxygen-sensitive fluorophore, ruthenium tris-(2,2'-bipyridyl) II dichloride, which has a 620 nm emission peak that is analyzed with an orange coated photodetector. Gallium phosphide 555 nm LEDs are used to excite the 550 nm absorbance peak of phenol red for pH measurement and phenol red in a 35 mM bicarbonate buffer for CO2 measurement. Accurate measurement of small absorbance and fluorescence changes using small bore capillary tubes allows good resolution of biochemical concentrations. Continuous replenishment of the indicator by flow lends itself to a stable method of biochemical analysis that has potential for long term performance. In vitro buffer studies demonstrate a resolution of plus or minus 0.5 mmHg for pCO2, a plus or minus 1.5 mmHg for pO2, and a plus or minus 0.003 pH.

  17. Chemical and Biochemical Engineering Getting Computing Help at Chemical and

    E-print Network

    Chemical and Biochemical Engineering Getting Computing Help at Chemical and Biochemical Engineering Help for Departmental Systems. Users of computing systems at Chemical and Biochemical Engineering can get technical support for most issues by contacting the Systems Administrator at help

  18. Toxicological assessment of P-9801091 plant mixture extract after chronic administration in CBA/HZg mice--a biochemical and histological study.

    PubMed

    Petlevski, Roberta; Hadzija, Mirko; Slijepcevi?, Milivoj; Jureti?, Dubravka

    2008-06-01

    Acute, subchronic and chronic effects of the P-9801091 plant mixture extract at a dose of 20 mg/kg body mass were assessed in serum of healthy CBA/HZg mice at 24 hours, 7 days, 3 months and 6 months of treatment (experimental group), and compared with the values obtained in the control group of untreated healthy CBA/HZg mice. The P-9801091 plant mixture extract is an antihyperglycemic preparation containing Myrtilli folium (Vaccinium myrtillus L.), Taraxaci radix (Taraxacum officinale Web.), Cichorii radix (Cichorium intybus L.), Juniperi fructus (Juniperus communis L.), Centaurii herba (Centaurium umbellatum Gilib.), Phaseoli fructus sine semine (Phaseolus vulgaris L.), Millefolii herba (Achillea millefolium L.), Mori folium (Morus nigra L.), Valerianae radix (Valeriana officinalis L.) and Urticae herba et radix (Urtica dioica L). Toxic effect of the P-9801091 plant mixture extract was assessed by the following biochemical parameters: urea, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cholesterol. Also, histopathological examination of the kidneys, liver, spleen, pancreas, testes and lungs was performed. Results of biochemical testing performed at specified time points generally showed no statistically significant differences from control values, with the only exception of the catalytic concentration of AST in the experimental group measured on day 7, which was significantly increased as compared with the control group (p<0.05). Pathohistological examination including characteristic organ and tissue structure, and parenchyma relationship to the adjacent blood vessels and connective tissue in the examined organs revealed no major pathologic changes. PMID:18756913

  19. Biochemical Characterization of Hypothetical Proteins from Helicobacter pylori

    PubMed Central

    Choi, Han-Pil; Juarez, Silvia; Ciordia, Sergio; Fernandez, Marisol; Bargiela, Rafael; Albar, Juan P.; Mazumdar, Varun; Anton, Brian P.; Kasif, Simon; Ferrer, Manuel; Steffen, Martin

    2013-01-01

    The functional characterization of Open Reading Frames (ORFs) from sequenced genomes remains a bottleneck in our effort to understand microbial biology. In particular, the functional characterization of proteins with only remote sequence homology to known proteins can be challenging, as there may be few clues to guide initial experiments. Affinity enrichment of proteins from cell lysates, and a global perspective of protein function as provided by COMBREX, affords an approach to this problem. We present here the biochemical analysis of six proteins from Helicobacter pylori ATCC 26695, a focus organism in COMBREX. Initial hypotheses were based upon affinity capture of proteins from total cellular lysate using derivatized nano-particles, and subsequent identification by mass spectrometry. Candidate genes encoding these proteins were cloned and expressed in Escherichia coli, and the recombinant proteins were purified and characterized biochemically and their biochemical parameters compared with the native ones. These proteins include a guanosine triphosphate (GTP) cyclohydrolase (HP0959), an ATPase (HP1079), an adenosine deaminase (HP0267), a phosphodiesterase (HP1042), an aminopeptidase (HP1037), and new substrates were characterized for a peptidoglycan deacetylase (HP0310). Generally, characterized enzymes were active at acidic to neutral pH (4.0–7.5) with temperature optima ranging from 35 to 55°C, although some exhibited outstanding characteristics. PMID:23825549

  20. Embedded Programmable Invasive Blood Pressure Simulator

    Microsoft Academic Search

    J. Kijonka; M. Penhaker

    \\u000a This paper is dealing with design and realization of programmable invasive blood pressure simulator. Parameters of the simulator\\u000a match with blood pressure sensor. The simulator is able to generate programmable behavior of voltage signal witch correspond\\u000a to blood pressure curve. The pressure curves can be monitored on patient monitors. Control unit of the simulator is a microcontroller.\\u000a Generated behaviors of